LOCUS       DAWKQB010000001        40971 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.001, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000001 DAWKQB010000000
VERSION     DAWKQB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40971
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.001"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            266..1198
                     /gene="pip"
                     /locus_tag="WJL14_000001"
     CDS             266..1198
                     /gene="pip"
                     /locus_tag="WJL14_000001"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ7655137.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNQLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEDVDEDAYASL
                     AIARLENHYFVNGGWLQGDKAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVQAVEDILPHLL"
     gene            complement(1255..2451)
                     /gene="bamC"
                     /locus_tag="WJL14_000002"
     CDS             complement(1255..2451)
                     /gene="bamC"
                     /locus_tag="WJL14_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ7655138.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKISQDSIRRLFDTVGLGGIYSTGERDKFIIRIEQGQNGVSDIFFAHK
                     AMKEVYGGKNKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKKPILPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAITE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYTEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2460..3335)
                     /locus_tag="WJL14_000003"
     CDS             complement(2460..3335)
                     /locus_tag="WJL14_000003"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ7655139.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLTEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQVDIALARELNNRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3642..5033
                     /locus_tag="WJL14_000004"
     CDS             3642..5033
                     /locus_tag="WJL14_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ7655140.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(5095..5847)
                     /gene="rlmB"
                     /locus_tag="WJL14_000005"
     CDS             complement(5095..5847)
                     /gene="rlmB"
                     /locus_tag="WJL14_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ7655141.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(5913..6401)
                     /locus_tag="WJL14_000006"
     CDS             complement(5913..6401)
                     /locus_tag="WJL14_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ7655142.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGL
                     ILGPFIGAAAGELIERRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6406..7125)
                     /locus_tag="WJL14_000007"
     CDS             complement(6406..7125)
                     /locus_tag="WJL14_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ7655143.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLNGTLPQEITQHDKTRLFAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(7129..8070)
                     /gene="miaA"
                     /locus_tag="WJL14_000008"
     CDS             complement(7129..8070)
                     /gene="miaA"
                     /locus_tag="WJL14_000008"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ7655144.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPTGSYSAARFVEDCTRLIGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQQVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSAHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFRQMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQTWEHLDGATDRQTFIEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            8250..8528
                     /locus_tag="WJL14_000009"
     CDS             8250..8528
                     /locus_tag="WJL14_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655145.1"
                     /translation="MSRNLLVRWLVVCLIPLATLAVFVANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIVLLVVYLFHYFGAF"
     gene            8547..9695
                     /gene="earP"
                     /locus_tag="WJL14_000010"
     CDS             8547..9695
                     /gene="earP"
                     /locus_tag="WJL14_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ7655146.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGGALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            9738..10298
                     /gene="efp"
                     /locus_tag="WJL14_000011"
     CDS             9738..10298
                     /gene="efp"
                     /locus_tag="WJL14_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ7655147.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(10348..11193)
                     /locus_tag="WJL14_000012"
     CDS             complement(10348..11193)
                     /locus_tag="WJL14_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ7655148.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(11310..11891)
                     /gene="metW"
                     /locus_tag="WJL14_000013"
     CDS             complement(11310..11891)
                     /gene="metW"
                     /locus_tag="WJL14_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ7655149.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            complement(11888..13027)
                     /locus_tag="WJL14_000014"
     CDS             complement(11888..13027)
                     /locus_tag="WJL14_000014"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ7655150.1"
                     /translation="MSQNASVGIVMPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWTISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            13170..13370
                     /locus_tag="WJL14_000015"
     CDS             13170..13370
                     /locus_tag="WJL14_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655151.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            complement(13478..13603)
                     /gene="ykgO"
                     /locus_tag="WJL14_000016"
     CDS             complement(13478..13603)
                     /gene="ykgO"
                     /locus_tag="WJL14_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ7655152.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(13603..13878)
                     /locus_tag="WJL14_000017"
     CDS             complement(13603..13878)
                     /locus_tag="WJL14_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ7655153.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            14079..14957
                     /gene="metF"
                     /locus_tag="WJL14_000018"
     CDS             14079..14957
                     /gene="metF"
                     /locus_tag="WJL14_000018"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ7655154.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15093..17369
                     /gene="metE"
                     /locus_tag="WJL14_000019"
     CDS             15093..17369
                     /gene="metE"
                     /locus_tag="WJL14_000019"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ7655155.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKNQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            17648..18385
                     /locus_tag="WJL14_000020"
     CDS             17648..18385
                     /locus_tag="WJL14_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ7655156.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            18634..20037
                     /locus_tag="WJL14_000021"
     CDS             18634..20037
                     /locus_tag="WJL14_000021"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7655157.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            20316..20693
                     /gene="sdhC"
                     /locus_tag="WJL14_000022"
     CDS             20316..20693
                     /gene="sdhC"
                     /locus_tag="WJL14_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ7655158.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            20687..21028
                     /gene="sdhD"
                     /locus_tag="WJL14_000023"
     CDS             20687..21028
                     /gene="sdhD"
                     /locus_tag="WJL14_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ7655159.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            21031..22794
                     /gene="sdhA"
                     /locus_tag="WJL14_000024"
     CDS             21031..22794
                     /gene="sdhA"
                     /locus_tag="WJL14_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ7655160.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREIMEIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            22914..23621
                     /locus_tag="WJL14_000025"
     CDS             22914..23621
                     /locus_tag="WJL14_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ7655161.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            23625..23873
                     /locus_tag="WJL14_000026"
     CDS             23625..23873
                     /locus_tag="WJL14_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ7655162.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            23985..25268
                     /gene="gltA"
                     /locus_tag="WJL14_000027"
     CDS             23985..25268
                     /gene="gltA"
                     /locus_tag="WJL14_000027"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ7655163.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            25473..28301
                     /locus_tag="WJL14_000028"
     CDS             25473..28301
                     /locus_tag="WJL14_000028"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ7655164.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMTLQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNASKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVLTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            28401..29582
                     /gene="odhB"
                     /locus_tag="WJL14_000029"
     CDS             28401..29582
                     /gene="odhB"
                     /locus_tag="WJL14_000029"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ7655165.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAETGVDVNALQGSGRDGRVLKEDVQNAAAKPAAATAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            29647..29838
                     /locus_tag="WJL14_000030"
     CDS             29647..29838
                     /locus_tag="WJL14_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655166.1"
                     /translation="MKLAALITLSFFVLTACDKEVIIVQEKSKAEKQADFEAQQKANC
                     AALSDKVRAKKSYCKKYEQ"
     gene            30098..31531
                     /gene="lpdA"
                     /locus_tag="WJL14_000031"
     CDS             30098..31531
                     /gene="lpdA"
                     /locus_tag="WJL14_000031"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7655167.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPTKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGEGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            31626..31913
                     /locus_tag="WJL14_000032"
     CDS             31626..31913
                     /locus_tag="WJL14_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655168.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            32012..33178
                     /gene="sucC"
                     /locus_tag="WJL14_000033"
     CDS             32012..33178
                     /gene="sucC"
                     /locus_tag="WJL14_000033"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ7655169.1"
                     /translation="MNLHEYQAKELLASYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVDNDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAGKIVAAVNA"
     gene            33189..34079
                     /gene="sucD"
                     /locus_tag="WJL14_000034"
     CDS             33189..34079
                     /gene="sucD"
                     /locus_tag="WJL14_000034"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ7655170.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(34250..34654)
                     /locus_tag="WJL14_000035"
     CDS             complement(34250..34654)
                     /locus_tag="WJL14_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7655171.1"
                     /translation="MDVQKQYSFETVAVSVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAHFPHAQNLSASKQRRHERGIWHRRFYEHTVRDETDLRRCANHIRSNPIKQKMSRA
                     ETETGTILQKIISVFSNAILIHSQTSNKACAT"
     gene            complement(35053..36123)
                     /locus_tag="WJL14_000036"
     CDS             complement(35053..36123)
                     /locus_tag="WJL14_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7655172.1"
                     /translation="MKEFVQEILIQYYPDNYSYIYNKSPLLQYLDSKMGAIYGNSKTR
                     RSLANIYAIYSLLHFYAESKFINKPEEYKKFYGFEYNKLFYFYRSLYGGDKLQNHALN
                     SRVNGEFKNKFNEELIIINNGKYLINIKYLYVDNFDLCQVSIDIIEKYIYLLKCKDSF
                     LVETIENLFESHDVSEKKRQISILLNEKSEARIFEIISYAILKNHYKNISVFFEYSRD
                     SLQEEFLKLYKTGRTNANDGGIDFVMKPLGRFFQVTEVNNFDKYLLDIDKVLHFPITF
                     VVKTETSKDQVNSELIEYINNKSNGMEVIINRYKNAIEEIITINELNDWLNELCNCDI
                     DKLLKDIDLYYKLELNLLANDE"
     gene            complement(36142..36999)
                     /gene="yhdJ"
                     /locus_tag="WJL14_000037"
     CDS             complement(36142..36999)
                     /gene="yhdJ"
                     /locus_tag="WJL14_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HEZ7655173.1"
                     /translation="MKKFEIQFNKNSTVILGDCLEVLKTLPSSSVDLIFADPPYGIGK
                     DFGNNKDFFADAYQYLDWCASWIDECMRVLKDNGTMYLMSSVQYMPILDRYVDEKYFI
                     INRIVWSYDSSGVQAKNKFGSTYEPILMFTHHKNSKYTFNSEDILIEAKTGAERKLID
                     YRKTPPQPYNAKKIPSNVWEFNRVRYKMDEYENHPTQKPEKLLERIILASSNKGDTIL
                     DPFSGSFTTSSVAVRLKRKAIGIEINLDYFKIGIRRTHISNEYNGEFLTKNKMRKTSN
                     KSKKDHSKT"
     gene            complement(37002..39854)
                     /gene="uvrA"
                     /locus_tag="WJL14_000038"
     CDS             complement(37002..39854)
                     /gene="uvrA"
                     /locus_tag="WJL14_000038"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ7655174.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPGRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIDRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIAVGTPEEVAEEKSSYTGKYLSMILKG"
     gene            40009..40806
                     /locus_tag="WJL14_000039"
     CDS             40009..40806
                     /locus_tag="WJL14_000039"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ7655175.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAVAAKIQAAVQDVLKD"
BASE COUNT         9715 a         9877 c        10926 g        10453 t
ORIGIN      
        1 ggttgctgtt atagtggatg aacaaaaacc agtacggcgt tgcctcgctt tagttcaaag
       61 agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta tctgtactgt
      121 ctgaggcttc gtcgccttgt cctgattttt gttaattcac tatatcgcga tttttcggca
      181 tttgcctttc ggggcggctt gtgtctcgtg cgtgatgttg cgtgtgggaa tgttcggatt
      241 gtcagaagca atatgggaga agatgatgta tgagataaaa cagccttttc atagcggata
      301 cttgcaggtg tctgaaattc atcaaattta ttgggaagag tcgggcaatc ccgacggtgt
      361 gccggtcatt tttttacatg gcgggccggg cgcgggggct tcgcctgaat gtcggggttt
      421 tttcaatccc gatgtgttcc gcatcgtcat catcgaccag cgcggttgcg gacgttcgcg
      481 cccgtatgct tgtgcggaag acaatacgac ttgggatttg gtggcggata ttgaaaaagt
      541 ccgtgaaatg ctgggtatcg ggaaatggct ggtgttcggc ggttcgtggg gcagtacttt
      601 gtcgctggct tatgcccaaa cccatccgga acgggtaaag ggattggtgt tgcgcgggat
      661 atttttgtgc agaccgtctg aaacggtgtg gctgaacgag gcgggcggtg tgagccggat
      721 ttatccggaa caatggcaaa aatttgtcgc gccgattgct gaaaatcggc ggaaccagct
      781 gattgaggcg tatcacggat tgctgtttca tcaagatgaa gaagtgtgcc tgtctgccgc
      841 gaaggcttgg gcggattggg aaagctatct gatccgtttc gagccggagg atgtggatga
      901 agatgcttat gcctcactgg cgattgcgcg tttggaaaac cattattttg tcaacggcgg
      961 ctggttgcag ggtgataagg cgattttgaa caatatcggc aaaatacggc atatcccgac
     1021 cattatcgtg caggggcggt atgatttgtg tacgccgatg cagagtgcgt gggagctgtc
     1081 gaaagccttt cccgaagcgg aattgagggt ggttcaggca gggcattgtg cgttcgatcc
     1141 gcctttggcg gatgcgttgg ttcaggcagt tgaggatatt ttgccccatt tgttgtaaaa
     1201 agttccgcat aaaaaagcag cttctgtttg gaagctgctt ttgttttgaa tggtttaacg
     1261 cagttcggaa tggagtttgc ccaataatgc ggatgcgtct ttgccggcat atgcgctgcc
     1321 gtctttgttg agcaggacga tgcgcgagcc gttggcgaca ggctcggtat agacaatcag
     1381 ttccggctgt tcggcaggtt tctccgcttt gcctttgccc agcaggcgtt tgaacaggcc
     1441 gggtttttgt tcggtaattg cattgctttc gttcggggct ttttgaacca ggaaggcgtg
     1501 gcgttcggtg ttttgaccga cgacggtcag cccgatgcgg tcgagggcga gcgcggtgcg
     1561 ccgccagttt ctgccgtagt cgccaaagac aatcaggctt ttgccttcga tacgcgccat
     1621 ttcgttggcg gcgggaagga tcggtttttt tgccgatgcg ttttccgcct gctgtccgtc
     1681 aacgcccaaa tattgcataa agcgcgtcag gaaagcggct tcgaggttgg gatcggacgg
     1741 ggagggctgc catacggtcg tgtctttgtt tttgccgccg tacacttctt tcatggcttt
     1801 gtgggcgaag aagatgtcgg aaacgccgtt ttgaccctgt tcgatgcgga tgatgaactt
     1861 gtcgcgttcg ccggtggagt agatgccgcc caagccgact gtgtcgaaga ggcggcggat
     1921 gctgtcttgc gagattttgg cgcggttttc cgcccactcg gtttccattt gtccgatggc
     1981 aggttcttcg gacttgatgt cgaagccgtt ttcctgccaa aaggctttca gaagcggcca
     2041 gatttcggca ggggatttgc cgtcaacgac aagccagcgt tggctgccgt cgcgctcgag
     2101 gcggacacct ttgacgcttt tcaatacctc ggcatcggca ggctgttgga cggcgggtgt
     2161 gcggcgtttt tccaaatcgc tggcgcggac ggcgcccgaa ccggcaggca ggcggtagag
     2221 gttgccttgg tcgggattgt tcaaatcggg cgggacttcg agtttgatca ggcggtgcga
     2281 ccggctttgg tagtcgagct tgggctgttc ggttttgctg ccggagcagg cggcaagccc
     2341 gatgagtgcg agcgcggcaa tgacgggttt gatatgggtc atcgtgtcat cctgtgtgat
     2401 ggatattaaa gtgtttgttg cgttatgccg tctgaacgct tcagacggca tggctatatt
     2461 taaagttgtc ctgaggcttt cagggcggcg cggacttttg cctgtccgcc ttccgtcagc
     2521 ggaacgagcg gcaggcggac gtgcggttcg catctgccca aggcggatac cgcccatttc
     2581 ggtgcggcgg ggctgggttc gcagaacatg gtgtcgtaaa tcggaatcag ccggttgttg
     2641 agttcgcgtg cgagggcaat gtcgacttgc agcgcggcgc ggcacatatc ggcaaagagc
     2701 ttgggcgcgg cgttggcggc tacggtaatc acgccgtgtc cgccgcacag catgaacggc
     2761 agggcggtat ggtcgtcgcc ggaaaggacg acgaagcctt cgggcgcgcg gttgatgagt
     2821 tcgatgttgc tgccgatgtt gccgctggct tctttcacgc cgacgatgtt ggggatttcg
     2881 gtaaggcgca ggatggtttc gttgttcatg ctgacgacgg ttctgccggg tacgttgtag
     2941 agtatcatcg gaatcgcggc ggcttcggca acggctttga aatggcggta catgccttct
     3001 tgcgaaggtt tgttgtaata ggggacgacg gagagggtgt agtccgcccc ggctttttcg
     3061 gcggcttggg aaagggcgat ggcttcgacg gtgttgtttg cccctgtgcc ggcgatgacg
     3121 gggacgcgtt tggcaacgtg tttaacgacg gcttcgatga cggcggtgtg ttcttcgacg
     3181 gagagggtgg cggattcgcc tgtcgtgccg acggcaacga tgccgtccgt gccgttttca
     3241 atgtgccagt cgattaagtc gcggagttgt tcgtaatgga tgctgccgtc ttgattcatc
     3301 ggggtaatca gggcaaccaa gctaccttgt aacatacaga accttttatc agttgtggtg
     3361 tagggggcgg taatgcttct gattgtagcc tactttaccg caggtgtgaa atccggcggg
     3421 tccgggatga gcggggcggc ttgacggcgg atgcgtcggg tttgtatttt tgtgtttgaa
     3481 atcaggttat tgcgttttgt aaacgatatt ccggcggcga cggttcggaa tctgtttgcc
     3541 ggacgggcgc gtatgccgtc cgaaatcgaa attgaagcgg tatggcggat tcgctacaat
     3601 ccgcgctttg gttttttcaa catttgaagc aaggaaacat tatgagcggt catatgggaa
     3661 aaggggcgga tgcgcctgat ttggtgtacg gtttggagga taggccgccg ttcggtaatg
     3721 cgctcttgag cgcggttacc catcttttgg cgatttttgt gccgatgatt acgcccgcgc
     3781 tgattgtggg cggcgcgctg gaattgccgg tggagatgac ggcgtatctc gtgtcgatgg
     3841 cgatggttgc gtcgggtgtc ggcacttatt tgcaggtcaa ccgcttcggg ccggtcggtt
     3901 cggggatgct gtccatccag tcggtgaatt tctcgttcgt taccgtcatg attgcgctgg
     3961 gcgcggggat gaaagagggc ggtttgacta aggatgcgat gatttcgacg ctcttgggcg
     4021 tatcgtttgt cggcgcgttt ttggtgtgtt tctcggcgtg gcttctgccg tatttgaaaa
     4081 aagtgattac gccgacggtc agcggtgtgg tggtgatgct gatcggcttg agtttggtac
     4141 acgtcggtat taccgatttc ggcggcggct tcggcgcaaa ggcggacggc acgttcggct
     4201 cgatggaaaa cttggggctg gcatcgctgg tgctgctgat tgtgctggtg ttcaattgca
     4261 tgaaaaaccc gctgctgcgg atgagcggca ttgcggtcgg tctgattgcc ggctatatcg
     4321 tcgcgctgtt tttgggcaag gtggattttt cggcactgca aaacctgccg ctggttacgc
     4381 tgcccgtacc gtttaaatat ggttttgctt ttgactggca cgcatttatt gtggcgggcg
     4441 cgattttctt gttgagcgtg tttgaggcgg tcggcgattt gaccgcaacg gcaatggtgt
     4501 ccgatcagcc gattgaaggc gaggaataca ccaaacgcct acgcggcggc gtgttggcag
     4561 acggcttggt gtcggtgatt gcgacggctt tgggttcgct gccgctgacg acgtttgcgc
     4621 aaaacaacgg cgtgattcag atgaccggcg tggcttcgcg ccatgtgggc aaatatattg
     4681 ccgtgatttt ggtgctgttg ggtctgttcc ccgttgtcgg acgcgcgttt acgacgattc
     4741 cgagtccggt gttgggcggc gcgatggttt tgatgttcgg cttaattgcg attgcgggcg
     4801 tgcggatttt ggtcagtcac ggcatccgca ggcgcgaagc ggtgattgcg gcaacgtcgg
     4861 tcggtttggg cttgggtgtc gcgtttgagc cggaagtgtt taaaaacctg cctgtcttgt
     4921 tccaaaactc tatttccgcc ggcggcatta cggcagtctt gctgaatttg gtcttgcccg
     4981 aagataaaac cgaggcggcg gtcaagtttg ataccgacca cttggaacac tgattttgaa
     5041 aatgaatgcc gtctgaaaca gaatccctgt ttcagacggc attgtttttg aggcttatac
     5101 tttttcgttt tttaatacgc gttgtcggcg tgtttcactt aataccattc cggcagacac
     5161 ggagacgttc atgctttcga ctgtgccgaa catgggtata gacaccagca tgtcgcaatg
     5221 ttcgcgcgtg aggcggcgca taccgtcgcc ttcgttgccc atcacccatg ccgcgctgtc
     5281 gggcagattg caatggtaaa ggtcggactc gccgctcata tcggtgccga tgatccaaat
     5341 gccgtattct ttcagctcgc gcagggtgcg ggcgaggttg gttacggtga tgtaggggac
     5401 ggtttccgcc gcgccgcagg cgactttgct gacggtggcg ttcagtcccg cgcttttgtc
     5461 tttcggtgcg atgacggcat gtacgcccat tgcgtcggcg gtacgcaggc acgcgccgag
     5521 gttgtgcgga tcggtgatgc cgtcgagtat cagcagcagc ggcggttcgc ttaagttttc
     5581 caatacgtct tcgaggtgga cgtggttttt ggaggcatcg ataaatccga ccacgccctg
     5641 atggcgcgcg cctttgctga tggcgttgag gcggtcggca tcggcaaaat atacgcggat
     5701 gttttcgttt gccgcctttt ccaacacttc gcgcgtgcgt gcgtcggatt tgccttcttg
     5761 gatgtagagt tcgacgatgg atttagggtt ttgccacaat cgggcgttga cggcgtgaaa
     5821 gccgtagatg ggtctttggt ttgccataat ggtgctttgt cggaaatttc agacggcatt
     5881 atagcaattt gccggtatgc cgtctgaaag ggttaaaaca ggtaggcgat gtatttcacc
     5941 aacaggataa acaagatgga tacggcgcag ccgattttga acgccgtgcc gacgacaagc
     6001 cccaacagcg tacccaagcc cgctttacct gcctgaagca tattgcgccg ttcgatcagt
     6061 tcgcctgccg ccgcgccgat aaagggaccg agtattagtc cgggaaggga gaaaaatatg
     6121 ccgatgatgc tgccggccaa tgcgccgcga acggcgagct tgcccgctcc ggtatatttt
     6181 gtcccccata tgcctgccac atagtccgcc agtatgccgg caaggctgat gagtccgacc
     6241 gtccacaaaa cgcccgcgcc gtagatttgg tagccgccgg cataggcaag cagccatgtt
     6301 ccggcaaaca tcaatgccaa tccgggcagg gcggggtaaa cgatgcccgc cgtgccgacg
     6361 gctatcaggg cgagggcgag gatgacggtc agtacggtca taggttcaac cttttctttt
     6421 gttttggaaa aaacggctta acacggcgcg gcattcttct tgcaggattc cgccccgtat
     6481 ggcggtgtgc gtattgaggc gtttgtcggc aaacaggttg acgatgctgc ctgccgcgcc
     6541 ggttttgggt tctgccgccc cgtagatcac gcgcctgatt cgtgcctgta tcagtgcgga
     6601 cgcgcacatg gcgcagggtt cgagggtgat atatatgtcg catccgtcaa ggcggtagtt
     6661 ttgcatttcg ctgcctgcct gtgccaaggc gttgatttcg gcgtgccggc tgacattgca
     6721 gtcggcaatg caggtgttgt gtgccgatgc gatgattttg ccgtctgaaa cgatgactgc
     6781 cccgacgggt atttcgccgt cggcggcgga ttgttctgct tggcgcagtg cttcgcgcat
     6841 gaagtgttcc atttcttcct gcggcggaaa ggcggcgacg ggcggatggt ttttcaattc
     6901 ggcaaaaagg cgcgttttgt cgtgttgcgt gatttcttgc gggagtgtgc cgttcagcag
     6961 cgactcaagc tgccacagcg tgcttttcgt gagggtcaaa cccgatgctt tgagcagcag
     7021 aaaggctttg accgaaccgt tttgccgcag ttcttcgagc gtacggatac cgagcctgtg
     7081 cagggcggcg acggttttgg gggcgagcgg cggtgtggtc agcatggttt atgcgccgaa
     7141 aaaccgtttt gccgcctcaa tcaggcgtgt gcatgaagtg ccgtctgaaa acgggtcggc
     7201 aacgcagtct aaaggtgttt tgcgcaacca agtcagttgg cgtttggcaa gttggcgggt
     7261 ggcggcaatg cctttctcga tgaaggtttg tctgtcggtt gcgccgtcga ggtgttccca
     7321 cgtctgacgg tagccgacac agcggatggc aggggagtcg gcggtcaggc cgggatagcg
     7381 gcggcgcagg ttttcgactt cgccgataaa gccctgttcg agcatttggc ggaaacgcag
     7441 ggcgatgttt tcgtgcagtc gggcgcggtt ttcgggaatc agggcggcgg tatgcaaatc
     7501 aaaagggagc gtatgggagg ttgggctgcc gaggtgcgca ctcatcggtt tgccggttaa
     7561 ataatagact tccaaagcgc gttcgatgcg ctggctgtcg ttcggtttca gacggcatgc
     7621 ggtttcggga tcgacctgtt gcagggtgcg atagaggaaa tccaagccgt acatttgttt
     7681 ttgttcgtcc aagtcggcac gcaggcaggc atcggcttcg ggcaaatcgt tcaaaccttg
     7741 ggtcagggcg cggaaataca tcattgtgcc gccgacaatc agcggacatt tcccacgcgc
     7801 cgtgatttcg ccgatcagac gcgtgcagtc ttcgacaaaa cgggcggcac tgtatgatcc
     7861 ggtaggcgta atgatgtcga taaggtggtg cgggacaaag gcgcgttcgg aggcggacgg
     7921 ttttgccgtg ccgatgtcca taccggtata gaccagcgcg gaatcgaggc tgatgatttc
     7981 gacaggcagg gtttcggcaa ttttgagggc gagcgcggtt ttgcctccgg cggtcggccc
     8041 gagcagggca aaggctttcg gggtcggcat aatgtttcag gtttggaaaa atgcggatta
     8101 tagcggaaag cgcgccgccc ttatattttg ctttgcggaa gcactgcgtt ttccggcggc
     8161 aaggcttcag acggcatctc cggcgcgtcc gttagacaag gcgtgcgctt ggggcgataa
     8221 tggcgttttg cttttttgaa agccttgcaa tgtcccgaaa cctgcttgtc cgctggcttg
     8281 tcgtctgcct catccctttg gcgacgcttg ccgttttcgt cgccaatccg cccgaagaca
     8341 aaccccagca tctgatcaac ggcatcatcc ttgcctgcga agcgacgttt ttgttcaaat
     8401 tcgtcctttt cgacaccatc aagcatcatt tgaaacaaga gtttgatttg aaacgtcaaa
     8461 ccatgttgct gtttattccg attgttttgc tggttgtgta tttgttccac tatttcggcg
     8521 cgttttagcc cgtttccgtt atttctatga atactcctcc ttttgtctgt tggatttttt
     8581 gcaaggtcat cgacaatttc ggcgacatcg gcgtttcgtg gcggctcgcc cgtgttttgc
     8641 accgcgaact cggttggcag gtgcatttgt ggacggacga tgtgtccgcc ttgcgtgcgc
     8701 tttgccctga tttgcccgat gttccctgcg ttcatcagga tattcatgtc cgcacttggc
     8761 attccgatgc ggcagatatt gataccgcgc ctgttcccga tgtcgtcatc gaaacttttg
     8821 cctgcgacct gcccgaaaat gtgctgcaca ttatccgccg acacaagccg ctttggctga
     8881 attgggaata tttgagcgcg gaggaaagca atgaaaggct gcacctgatg ccttcgccgc
     8941 aggaaggtgt tcaaaaatat ttttggttta tgggtttcag cgaaaaaagc ggcgggttga
     9001 tacgcgaacg cgattactgc gaagccgtcc gtttcgatag cgaagccctg cgtcagcggc
     9061 tgatgcttcc cgaaaaaaac gcccccgaat ggctgctttt cggctatcgg agcgatgttt
     9121 gggcaaagtg gctggaaatg tggcgacagg caggcagccc gatgacactg ttgctggcgg
     9181 ggacgcaaat catcgacagc ctcaaacaaa gcggcgttat tccgcaagat gccctgcaaa
     9241 acgacggcga tgtttttcag acggcatccg tccgcctcgt caaaatccct ttcgtgccgc
     9301 aacaggactt cgacaaactg ctgcacctcg ccgactgcgc cgtgatacgc ggcgaagaca
     9361 gtttcgtgcg cgcccagctt gccggcaaac ccttcttttg gcacatctac ccgcaagacg
     9421 agaatgtcca tctcgacaaa ctccacgcct tttgggataa ggcacacggt ttctacacgc
     9481 ccgaaaccgt gtcggcacac cgccgtcttt cggacgacct caacggcgga ggggctttat
     9541 ccgccacgca acgcctcgaa tgttggcaaa tcctgcaaca acatcaaaac ggctggcggc
     9601 aaggcgcgga ggattggagc cgttatcttt tcgggcagcc ttccgcatcc gaaaaactcg
     9661 ccgcctttgt ttcaaagcat caaaaaatac gctagaatag tgcgttttac gacaaccgat
     9721 ttgattggaa aatcacaatg aaaacagcac aagaactgcg cgccggcaat gtatttatgg
     9781 tcggcaacga tcctatggtc gttcaaaaaa ccgaatacat caaaggcggc cgctcttccg
     9841 ccaaagtcag catgaaactg aaaaacctgc tgaccggcgc ggcttccgaa accatttaca
     9901 aagccgacga caaattcgac gtggtcatcc tgtcccgcaa aaactgtacg tacagctact
     9961 ttgccgatcc gatgtacgtc tttatggacg aagaattcaa ccaatacgaa atcgaagccg
    10021 acaacatcgg cgacgcgttg aaattcatcg ttgacggcat ggaagaccaa tgcgaagtta
    10081 ccttctacga aggcaacccc atctccgtag aactgcccac catcatcgta cgcgaagtcg
    10141 agtataccga gcctgccgtc aaaggcgata cttccggcaa agtgatgaaa accgcgcgcc
    10201 tggtcggggg caccgaaatc caagtgatgt cttacatcga aaacggcgac aaaatcgaaa
    10261 tcgacacccg cactggcgaa ttccgcaaac gcgcttgatt tgttgcattg aaaaatgccg
    10321 tctgaaaacg tttcagacgg cattttttta tcttcgcccc aagtttggat tgaagtagat
    10381 gtttttttcg taaacgacaa tacgcgtgat tttgccattt tcgtcaaaat ggatgtctaa
    10441 aaacggtttg tcgggattgc gttcacggtt ggacagccgg aagagtgatt ggttttcaag
    10501 catttctccg tgtattttca gatagccggg caattggccg atgtattgga aattgccgcc
    10561 aagtccgttg ttgtgccggc tcgaataggc aggggttgcc gaaacttcaa aataacgcgt
    10621 gcgtttcggc tcctcgcctt gtccgcgggt ttcttgactg ccctgtatca gcaacagcgt
    10681 cgcttctcgg aggcggcgtt cctgttccat caaggcatct tgtgcctgcc ggctgatcgt
    10741 gcgctttccg taacgcaagt cttcaattgc cagtatgatt ttctgatggt agtcgggatc
    10801 ttcagggcgg atgtcgggaa acagcagccg ctgtatgttg tcatagccgc cgccgggtaa
    10861 gccgaaacgg gtttccagtt cgtgatggga aagggcaaac agacagtccg agtcaatgtg
    10921 ttcggcgatt tgctgcatga gatcgtcaat gccggtggcg gggtgcaggt cggtgcaatt
    10981 tgacggcggc acgctttctg cctgcttgac gttatcaggg gcggatgcgg ccggttgcaa
    11041 atcggtttgg ggcgcggaag acgaagcggc ggatgccgtg gacgaggcgg cggatgcgcc
    11101 gttttctttc gttttatctt ctttgctcgg ggaacacgcg gtcagcatca ttgcggcaag
    11161 ccataagaga agtgatgagg ttttgttttt cattctattg tttccagtat taaagaggcc
    11221 gtctgaaaac ctaccgtttc atttttcaga cggcctgttg ttaatagaac cgaagaacct
    11281 gttaatgccg acaaggttct caatctgtct tacccgacgc ggtaaaatgc aaggctgccc
    11341 aaaaggttgg ggaaatgttt gacctgtctg ttgcccgtca tcacggcgcg ctcaagcacg
    11401 cggatgttgt ttttggcgca caacaaatca aagtctttga gcgtgcacca atggatattg
    11461 ggcgtgtcgt accaatggta gggcatacgt tcggaaaccg gcatatgtcc gccgagtgcg
    11521 atttggacgc ggttgcgcca gtagccgaaa ttcgggaagc tgacgattgc ctgttttgcc
    11581 acgcgcatca ggcagcgcag gattttttcg gtattctgca tcgcttggat ggtctggctc
    11641 aacacaatca catcaaaact ttgatcgttg aatgcggtta aaccttcttc caaatcggct
    11701 tggataacat tcacgccgcg cgacatcgcg gcaatcacgc tgtcggtgtc gatttcgatg
    11761 ccgtagccgc tgcatttttt gtgttcgacc aaggcggcaa gcagttcgcc gtcgccgcag
    11821 cccaagtcca agacgcggct gccttcgggt atccggtcgt aaatcagttg caaatcatcg
    11881 cgcaggttca ttgctgacat tccttataaa cgttgttcat ataggcggcg accgcacgca
    11941 tataggcttc gtcttccatt aaaaaggcat cgtgcccgtg tgcggatttg acttcgatat
    12001 actgcacgga tttttgggcg gcaatcaggg ccttgaccag ttcgtgcgaa cgttcgggcg
    12061 cgaaacgcca atcggtgctg aagctggcga caaagaattt tgcctgaaca tcctgcacgg
    12121 cgcgggtcag gctgttgccg aaatccgccg ccggatcgaa atagtccaaa gccttggtca
    12181 tcagcaaata ggtgttggca tcaaaccgcc cgacgaattt gtcgccttga tagcgcagat
    12241 aggattctac ttcaaattca acgccatagc cgtattgata gccgttggaa cgcaaatcgc
    12301 gtccgaattt tttgcccaaa ccgtcttcgg caagataggt gatgtgtccc atcatacggg
    12361 caatccgtaa gccccgtgca ggaacggtat tatggctgcg gtaatgtcct tcgttgaaat
    12421 cggggtcggt caaaatcgcc tgacgcgcca catcgttaaa cgcgatattt tgcgtagaaa
    12481 gcttcggcgc agacgcaatc actaaagcat ggcgcacgcg ttcgggatag gaaatcgtcc
    12541 actgcaaagc ctgcataccg cccaagctgc cgccgacaac cgccgcccat tgcccgatgc
    12601 cgagataatc ggcaagcgcg gcttgggatt ttacccagtc cttcaccgta accagcggaa
    12661 aatccgcgcc gtattcccta cccgtttcag gattgatcga caaaggcccg ctgctgccgt
    12721 cgcagccgcc cagattattc aaaccgacca cgaaaaaacg ttccgtatca atcggtttgc
    12781 caggtccgac catattgtcc caccagcccg tatatttatc ttccgccgaa tgcctgcccg
    12841 caacatgatg gttgcccgac agcgcgtggc agattaaaac cgcattgttt ttttcagcat
    12901 tcagctcgcc gtaggtttca atcatcagat cgaaacgcgg caaagtttta ccgttttcca
    12961 aaaccagcgg cacctcaaac ggaatttttt ggggcattac aatgcccacc gaggcatttt
    13021 gactcatatc ctgttccaac aaatacggca aaaagcgtta ttatatcgca aacggcatga
    13081 ctttttgaca cggtcggaca agcagcctga cgcgctgaaa catccttccc catgccgccg
    13141 cgcccgaacc atacttttcg cttcccgaca tgataggcag acttttccgt atttttttct
    13201 ttttcgcact tgccgcgctg attatgaacc gcctcttcag ccgcaggcag aaacgcgccc
    13261 tgcgcgaagt tgccgaaatc agtgcccggg tgctgctcgg tgcggccgcc gcgacgctgt
    13321 tttggtatct gtttatgctg tatttcaaac acattccgga ttcgtattga cggaaaaaat
    13381 gccgcctgaa acgcattttt ctgtttcaga cggcatattt gatgaaaagg gcttgcggta
    13441 ggaggggatt tacaaccgaa gcaggaaggg cagggagtca gcgttggcgt gctttaaaac
    13501 gcggattgct tttgcagatg acgtaaactt tgcccctgcg cctgacgatt tggcagtcgc
    13561 ggtggcgttg tttggcggtt ttgagtgaag acagaacctg cattatttgt cctttctaaa
    13621 cgatgacatg acggattgga aacgttggtt gaatttgctg gcacggcctt cggtgttgac
    13681 gttgcgctgt ttgccagtat agacgggatg ggatgcggaa gaagtatcca gcgaaaacag
    13741 cggatattct ttgccgtctg tccaaaccat cgtttttccg tgtgtttcgg cgcaagaacg
    13801 aatcaaccag ccttcattgg cgctgctgtc gaaaaaaagg acggttcgat aattgtcagg
    13861 atgaatattc ggtttcatat atcgccttag ttgaattgtt ataacataac aaactttatc
    13921 ataaattaga aggcttgtac aaggaaattt aactattttt gtaatatatt agaaattttc
    13981 atgataaatc tgaaaatttt gaaattgact catgtttggc gcaactttat tatgttgcct
    14041 gaaaatatca tataaaagat aataaaaggt acgcagccat gaattacgca aaagaaatca
    14101 atgcgttaaa caacagcctt tccgatttga aaggcgacat taacgtttcg tttgaatttt
    14161 tcccgccgaa aaacgaacaa atggaaacca tgctgtggga ttctatccat cgcctgcaaa
    14221 ccttgcaccc gaaatttgtt tccgtaactt acggtgcaaa ctcaggcgag cgcgaccgca
    14281 cgcacggcat cgtcaaacgc attaagcaag aaaccggctt ggaagccgcg ccgcacctga
    14341 ccggcatcga cgcttctccc gacgaattgc gccaaatcgc caaagactat tgggacagcg
    14401 gcatccgccg cattgtcgcc ctgcgcggcg acgagccgcc cggttacgag aaaaaaccgt
    14461 tttatgccga agacttggtt aagctattac gctccgtcgc cgacttcgac atctctgtag
    14521 cagcatatcc cgaagtgcat cccgaagcga aatccgcaca agccgacctg attaatttga
    14581 aacgcaaaat cgatgcgggc gcgaaccacg tcatcaccca atttttcttt gacgtagaac
    14641 gctacctgcg cttccgcgac cgctgcgtga tgttgggtat cgatgtggaa atcgtccccg
    14701 gtattttgcc tgttaccaac ttcaagcagc tcggtaaaat ggctcaagta accaacgtca
    14761 aaatcccaag ctggctgtcg caaatgtatg aaggtttgga cgacgaccaa ggcacgcgca
    14821 atctggtggc ggcaagtatc gccatcgata tggtcaaagt cctgtcccgc gaaggcgtga
    14881 aagatttcca cttctatacg cttaaccgca gcgagctgac ttacgccatt tgccatattt
    14941 taggcgtgcg cccttaaagc tgaaaacggc ttcagacggc atccgaggcc gtttgaaaag
    15001 caaaacgccg ccccatccta gccattctga tttacaatac cggccgattc ggattgaacc
    15061 ggtccttaca aaatccaact ggagagttca acatgacaac attacatttc tcaggcttcc
    15121 cgcgtgtcgg cgccttccgc gaattgaaat tcgcacaaga aaaatactgg cgcaaagaaa
    15181 tcagcgagca agaattgctg gctgttgcta aagacttgcg cgagaaaaac tggaaacacc
    15241 aggccgctgc caacgccgat ttcgttgccg taggcgattt cactttctac gaccacatcc
    15301 tcgacctgca agtcgccacc ggcgcgattc ccgcccgctt cggtttcgac agccaaaacc
    15361 tgtctttgga acaattcttc caactggcgc gcggcaacaa agaccaattc gctatcgaaa
    15421 tgaccaaatg gttcgacacc aactaccact acttggtgcc tgaattccac gccgataccg
    15481 aattcaaagc caatgccaaa cactatgttc aacaactgca agaagcccaa gccctcggtc
    15541 tgaaagccaa accgaccgtt gtaggtccgt tgactttcct gtgggtgggt aaagaaaaag
    15601 gcacagtcga attcaaccgc ctgagcctgt tgcaaaaact gctgcctgtt tacgttgaaa
    15661 tcctaaacgc tttggttgaa gccggtgcag aatggattca aatcgacgag cctgctttga
    15721 ctgtcgatct gcctaaagaa tgggtagaag cctacaaaga cgtttacgct actttgagca
    15781 aagtaagtgc caaaatcctg ctgagcactt acttcggttc tgttgccgaa cacgccgcat
    15841 tgttgaaatc cctgcctgtt gacggcctgc acatcgactt ggtacgcgcg cccgagcaac
    15901 tggacgcatt tgccgactac gacaaagttc tgtctgcagg catcatcgac ggccgcaaca
    15961 tctggcgcgc caacctgaac aaagttttgg aaactgttga gcctctgcaa gccaaactgg
    16021 gcgaccgttt gtggatttcc agctcttgct ctctgctgca cactccattt gacttgtcag
    16081 ttgaagaaaa actgaaagcc aacaaacccg acctgtactc ttggttggca ttcaccctgc
    16141 aaaaaaacca agaattgcgc gttctgaaag ctgcattgaa cgaaggccgt gattctgttg
    16201 ccgaagaact cgccgccagc caagctgctg ccgactcccg tgccaacagc agcgaaatcc
    16261 atcgtgcaga cgttgccaaa cgcctggccg atttgcctgc caacgcagac caacgcaaat
    16321 ctccatttgc cgaccgtatc aaagcgcaac aagcatggtt gaacctacct ctgctgccga
    16381 cgaccaacat cggttctttc ccgcaaacca ccgaaatccg ccaggcacgc tcagccttca
    16441 aaaaaggcga actgtctgcc gccgattacg aagccgcgat gaaaaaagaa atcgccttgg
    16501 tggttgaaga gcaagaaaaa ctggacttgg acgtactggt acacggcgaa gccgagcgta
    16561 acgacatggt tgaatacttc ggcgaattgt tgagcggttt tgcattcact caatacggct
    16621 gggtacaaag ctacggctca cgctgcgtga aaccaccgat tatctttggc gacgtaagcc
    16681 gtcctgaagc catgaccgtg gcttggtcta cttacgcaca aagcctgacc aaacgcccga
    16741 tgaaaggtat gttgaccggc cctgtaacca ttctgcaatg gtctttcgtc cgcaacgaca
    16801 ttcctcgctc taccgtgtgc aaacaaatcg cactggctct gaacgacgaa gtattggatc
    16861 tggaaaaagc cggcatcaaa gtcatccaaa ttgacgaacc tgccatccgc gaaggcttgc
    16921 cgctgaaacg cgccgattgg gatgcctacc tgaactgggc gggcgaatcc ttccgcctgt
    16981 cctctgccgg ttgcgaagac agcacccaaa tccacactca tatgtgttac tccgagttca
    17041 acgacatcct acctgccatc gccgctatgg atgctgatgt catcaccatc gaaacttcac
    17101 gttccgatat ggaactcttg accgcgttcg gcgaattcaa atacccgaac gacatcggcc
    17161 cgggcgttta cgacatccac agcccgcgcg taccgactga agctgaagtt gagcatctgt
    17221 tgcgcaaagc catcgaggtt gtaccggttg aacgtctgtg ggttaaccca gactgcggcc
    17281 tgaaaacccg tggctggaaa gaaactctgg aacaactcca agtgatgatg aacgtaaccc
    17341 acaaactgcg tgccgaactg gcgaaataag cagggataac gtgatcggat gtcgtctgaa
    17401 tacttttcag acggtatttt gtcctgattt gcggcgcaag ggcgcagttg ccggaaaatc
    17461 ttttcattgc agcttgtttt tttctaattc ggctttatat gtgggaaaca ggcaaatcgg
    17521 agttgtgttt gatagtttta agtaatttat attatttgaa ctataaatta tacaaatcat
    17581 tttgcatggg gtagaatgcc cagcgattca caattatttc tcaaaccaat ctattaagga
    17641 gcttaaaatg gctttgcaag atcgtaccgg tcaaaaagta ccttccgtag tattccgcac
    17701 ccgcgtcggc gacacttgga aagatgtgtc taccgatgat ttgttcaaag gcaaaaaagt
    17761 agtcgtattc tccctgcccg gtgcatttac cccgacttgt tcttcttcac acctgccgcg
    17821 ttacaacgaa ttgttcggcg cgttcaaaga aaacggcgtt gacgcaatct actgcgtatc
    17881 tgtaaacgat acgttcgtaa tgaacgcttg ggctgccgaa gaagaatccg acaacatcta
    17941 catgattcct gacggcaacg gcgaatttac cgaaggtatg ggtatgctgg tcggtaaaga
    18001 agacttgggc ttcggtaaac gctcttggcg ttactccatg ctggttaacg acggcgtggt
    18061 tgaaaaaatg ttcatcgaac ctgaagaacc gggcgatcct ttcaaagtat ccgatgcaga
    18121 tactatgctg caattcgttg ctcccgattg gaaggctcaa gagtctgttg ccatctttac
    18181 caaacccggc tgccaattct gcgccaaagc caaacaagct ttgcaagaca aaggtttgtc
    18241 ttacgaagaa atcgtattgg gcaaagatgc aaccgttact tccgttcgcg ccattaccgg
    18301 caagatgact gctcctcaag tcttcatcgg cggcaaatac atcggcggca gcgaagattt
    18361 ggaagcttac ttggctaaaa actgatagct gtttgcttaa ggcggtttga ttaaactgtc
    18421 tgatataccg gatagagtta ttcgggcggt tctacactgc cgatccgaat aactctatat
    18481 ttataagaga atttggatat tgttgcactc aatcgaaatt ttgtttttat ttatctgaat
    18541 gatgtttttg attgggaaaa tatttaaatg ccgtctgaaa ccgatatgtt ctgtgtcggc
    18601 aatgtttcag acgaaaacgg aaggacaaag attatgaaaa aaattcaagc ggatgtcgtc
    18661 gtaatcggcg gcggtactgc cggtatgggt gcgtttcgca atgcccgttt acattcggat
    18721 aatgtttacc tgattgaaaa caatgtgttc ggcacgacct gcgcgcgcgt gggctgtatg
    18781 ccttccaaac tcttgattgc cgccgcagag gcgcgtcatc acgcattgca taccgacccg
    18841 ttcggcgtgc atttggacaa agacagcatc gtcgtcaacg gcgaagaggt catgcagcgc
    18901 gttaaatccg agcgtgaccg ttttgtcggc tttgtcgttg ccgatgtgga agagtggcct
    18961 gccgacaagc gcattatggg ttcggctaaa tttatcgacg agcataccgt ccaaatcgac
    19021 gagcatactc aaattacggc aaaaagtttc gtgattgcta ccggttcgcg tcccgtcatc
    19081 ctgccgcaat ggcagtcttt gggcaatcgt ttgattatca acgatgacgt tttctcatgg
    19141 gatacgctgc ctaagcgcgt tgccgtgttc gggccgggtg ttatcggttt ggaactgggt
    19201 caggcattgc accgtttggg cgtgaaagtt gaaattttcg gtttgggcgg aatcatcggc
    19261 ggcatttccg accccgtcgt ttcagacgag gcgaaagccg tgttcggcga agaattgaaa
    19321 ctgcatctgg atgctaaaac cgaggtcaaa ctagatgcag acggcaatgt agaagtccat
    19381 tgggagcagg acggcgaaaa aggcgtattt gttgccgaat atatgctggc agccgtgggc
    19441 cgccgtccga acgttgacaa tatcggtttg gaaaacatca atatcgaaaa agacgcgcgc
    19501 ggcgtacccg ttgccgaccc actgaccatg cagaccagca ttccgcatat ctttattgcg
    19561 ggcgacgcgt ccaaccaact gcctctgctg catgaagctg ccgaccaagg caagattgcc
    19621 ggcgataacg cgggccgcta cccgaatatc ggcggcggtt tgcggcgcag caccatcggc
    19681 gtggtgttta ccagtccgca aatcggcttt gtcggtctga aatacgcgca ggttgccgcg
    19741 caataccaag ccgacgaatt tgtcatcggc gaagtatcgt tcaaaaacca aggccgcagc
    19801 cgcgtgatgc tggtgaacaa aggccatatg cgcctgtatg ccgaaaaagc caccggccgc
    19861 tttatcggcg cggaaatcgt aggccctgcc gccgaacatt tggcgcactt gctggcatgg
    19921 gcgcatcaaa tgaagatgac cgttccgcaa atgctggata tgccgttcta ccatcccgtt
    19981 atcgaggaag gtctgcgtac cgcgttgcgc gatgccgatg cgaaattgaa agcctgaccg
    20041 atatggcaaa acaatgccgt ctgaaatttt ttcagacggc attttgtttt tggggatggg
    20101 gtcggatgct gataccgtgt cgggaagggg gcggcaaaac taaaaatctt tcttttaatc
    20161 tgctgtttcc acgcgtgttt gtcaaaatct atcagtttgt ttttaaaata cactgttcaa
    20221 aatgggataa aacaggtaaa ttaacgttta tgtaacccag tgtagcaatg ggtttacggt
    20281 ttttgagtcg atatataact acagaggaat tgactatgtc tgccaaaccg cgtcctgttt
    20341 atctagattt gccgaacatc cgtctgccga tacccgggat agtttccatc cttcaccgca
    20401 tcagtggggt cgggctgttt attatgctgc ctttcctgct gtatttcctg tccggtaccc
    20461 tgagtcaaga gtctgcattt gaaacttacc gtgccattgt ttcccatcct ttggtcaagc
    20521 tggttttaat cggtgtattg tgggcttatc tgcaccattc tctcgccggt atccgctttt
    20581 tatttttgga tgcacacaaa ggccttgagc tgaatactgc gcgcaatacc gctaaagccg
    20641 tatttgcttc tgcattggtt ttgactgtcg ttttgggagc gttgttatgg tagaacgtaa
    20701 attgaccggt gcccattacg gtttgcgcga ttgggcgatg caacgtgcga ctgcggttat
    20761 tatgttgatt tataccgttg cacttttagt ggttctattt tccctgccta aagaatattc
    20821 ggcatggcag gcatttttta gtcaaacttg ggtaaaagta tttacccaag tgagcttcat
    20881 cgccgtattc ttgcacgctt gggtgggtat ccgcgatttg tggatggact atatcaaacc
    20941 cttcggcgtg cgtttgtttt tgcaggttgc caccatcgtc tggctggtcg gctgcttggt
    21001 gtattcaatt aaagtaattt gggggtaagt atgggttttc ctgttcgcaa gtttgatgcc
    21061 gtgattgtcg gcggtggtgg tgcaggttta cgcgcagccc tccaattatc caaatccggt
    21121 ctgaattgtg ccgttttgtc taaagtgttc ccgacccgtt cgcataccgt agcggcgcag
    21181 ggcggtattt ccgcctctct gggtaatgtg caggaagacc gttgggactg gcacatgtac
    21241 gataccgtga aaggttccga ctggttgggc gaccaagatg cgattgagtt tatgtgccgc
    21301 gccgcgcctg aagccgtaat tgagttggaa cacatgggta tgccttttga ccgtgtggaa
    21361 agcggtaaaa tttatcagcg tcctttcggc ggccatactg ccgaacacgg taaacgcgcg
    21421 gtagaacgcg cctgtgcggt tgccgaccgt acaggtcatg cgatgctgca tactttgtac
    21481 caacaaaacg tccgtgccaa tacgcaattc tttgtggaat ggacggcaca agatttgatt
    21541 cgtgatgaaa acggcgatgt cgtcggcgta accgccatgg aaatggaaac cggcgaagtt
    21601 tatattttcc acgctaaagc tgtgatgttt gctaccggcg gcggcggccg tatttatgcg
    21661 tcttctacca atgcctatat gaataccggc gatggtttgg gtatttgtgc gcgtgcaggt
    21721 atcccgttgg aagacatgga attctggcaa ttccacccga ccggcgtggc aggtgcgggc
    21781 gtgttgatta ccgaaggcgt acgcggcgag ggcggtattc tgttgaatgc cgacggcgaa
    21841 cgctttatgg aacgctatgc gccgaccgta aaagacttgg cttctcgcga cgttgtttcc
    21901 cgcgcgatgg cgatggaaat ctacgaaggt cgcggttgcg gtaaaaacaa agaccatgtc
    21961 ttactgaaaa tcgaccatat cggcgcagaa aaaattatgg aaaaactgcc gggcatccgc
    22021 gagatttcca ttcagttcgc tggtatcgat ccgattaaag acccgattcc cgttgtgccg
    22081 actacccact atatgatggg cggcattccg accaattacc acggcgaagt cgttgtgccg
    22141 caaggagacg aatacgaagt gcctgtaaaa ggcctgtatg ccgcaggtga gtgcgcctgt
    22201 gcttccgtac acggtgcgaa ccgtttgggt acgaactccc tgctggactt ggtggtattc
    22261 ggtaaagctg ccggcgacag catgattaaa ttcatcaaag agcaaagcga ctggaaacct
    22321 ttgcctgcta atgctggtga gttgacccgc caacgtatcg agcgtttgga cagccaaacc
    22381 gatggtgaaa acgttgatgc attgcgccgc gaactgcaac gctctgtaca actgcacgcc
    22441 ggtgtgttcc gtaccgatga gattctgagc aaaggcgttc gagaaattat ggagattgcc
    22501 gagcgcgtga aacgtaccga aatcaaagac aagagcaaag tgtggaatac cgcgcgtatc
    22561 gaggctttgg aattggataa cctgattgaa gtggcgaaag cgactttggt gtctgccgaa
    22621 gcacgtaaag aatcacgcgg tgcgcacgct tcagacgacc atcctgagcg cgatgatgaa
    22681 aactggatga aacatacgct gtaccattca gataccaata ccttgtccta caaaccggtg
    22741 cacaccaagc ctttgagcgt ggaatacatc aaaccggcca agcgcgttta ttgatgcgtt
    22801 ttcagacggt cttcgcctca aaggtcgtct gaaatctaac catacccaca ttgaactgct
    22861 tgaatttata atacaaaatc attgggcagt ttatgagaaa aggaacactt ctcatggaaa
    22921 aaatgagttt tgaaatttac cgttacaacc cggacgttga tgccaaacct tatatgcagc
    22981 gttacgagtt ggaattggaa ccaaccgacg ttaaactttt ggacgcttta gtgcgcctga
    23041 aagcacaaga tgatacattg tctttccgcc gttcctgccg cgaagggatt tgcggttctg
    23101 acggtatgaa catcaacggc aaaaacggct tggcatgttt gaccgatctg cgtggcttga
    23161 aacagccggt taaaatccgt cctctgccag gtctgcctgt tatccgcgac ctgattgtgg
    23221 atatgaccca gttcttcaaa caataccatt ccgtcaaacc ttatgttgtc aacgataatc
    23281 cgattgatgc ggacaaagag cgtctgcaaa ctcaggaaga gcgtaaagag ttggacggtt
    23341 tgtacgagtg tattttgtgc gcctgctgtt cgactgcctg cccgtcattt tggtggaacc
    23401 ctgataaatt cgttggtccg tccggtttgc tgaatgctta ccgtttcatt gcggacagcc
    23461 gtgataccat cactaatgag cgtttggata atctgaacga cccataccgt ttgttccgtt
    23521 gccacaccat tatgaactgc gtagacgtat gtcctaaaca cttgaatccg acccgagcca
    23581 tcggtaagat taaagagatt atgttgaaac gggccgttta agaaatgatg gtttttgacg
    23641 atattgccaa acggaaaatc cgttttcaaa cccgccgggg attgttggag ttagatttaa
    23701 tcttcggcag gtttatggaa aaagaattcg agcatttgag cgataaagag ctgtccgagt
    23761 tttccgaaat ccttgaattt caagatcaag aattgcttgc cttgattaac gggcattcgg
    23821 aaacggacaa agggcacctt atcccgatgc ttgaaaaaat cagacgggca tgaggcatct
    23881 gaaatctgcc tgcaggcaga tttcaaaatg caaaagccgt ctgagggcaa agaacgtgct
    23941 gcggatgcag taatgtgggt tataacttgc aaaggagcaa taatatgtcc aaatcaatta
    24001 aactcaacgt accgggtcag gcaggtttgg agctgccggt attggaagcc agcatcgggc
    24061 acgatgtggt tgacattcgg gggctgacaa aaaatacagg tttgttttcc ttcgaccccg
    24121 gatttgtttc aaccgcaagc tgtgagtcta aaattactta catcgacggc gatcaaggct
    24181 tgctttatta tcgcggatac cccatcgagc agctggccga aaagtccgat tatttggaag
    24241 tctgctacct gttgatttac ggcgaactgc cgactcccga gcaaaaggca gaatttgaca
    24301 ataccgtccg ccgccacacg atggtgcatg aacagctgac ttggttcttc cgggggttcc
    24361 gccgcgacgc gcatccgatg gcgatgatgg tcggcgtggt cggcgcgctg tctgcgttct
    24421 accaagacag cttggacatt agcaatcccg aacaccgcaa aatcgcgatt taccgcctga
    24481 tttccaaaat cccgaccatt gcggcaatgt gctaccgcta ttcaaacggt ctgccgttca
    24541 attatccgaa gaataatctt tcttattccg aaaacttcct tcatatgatg ttcgccacgc
    24601 cgtgtgaaga ctacaaaccc aatcccgttt tggcacgcgc gctcgaccgc atctttattt
    24661 tgcatgccga ccacgagcaa aacgcctcaa cttcaaccgt ccgtctggca gggtcttcgg
    24721 gtgcgaatcc gtttgcctgt attgctgccg gtatcgcctg cctgtggggt gcttcgcacg
    24781 gcggtgcgaa cgaagccgtg ttgaaaatgt tggacgaaat cggcgatgtg tctaatgttg
    24841 ccgcatacat ggaaggtgtg aaacaacgca aataccgtct gatgggcttc ggtcaccgcg
    24901 tgtaccgcaa tatggatccg cgtgccagca ttatgcgcga aacctgctat gaagttttga
    24961 aggaattggg cttggaagac agtccgaaat tcaaactggc gatggaattg gaacagattg
    25021 cgctgaaaga cccgttcttt atcgaacgca agctgtatcc aaacgtcgat ttctattccg
    25081 gcatcgtcct gtccgcgctg ggcatcccga ccgaaatgtt taccgtcatc ttcgccctgt
    25141 cgcgcagcgt aggttggatt tcgcactggc acgagatgat cagcgatcct tcgctgaaaa
    25201 tcggccgccc gcgccagctt tataccggtt cggaacgccg cgattatgtg ccggcaggag
    25261 agaggtaaca tacattaata tattgtcaaa caggcaatat tagagaatcg gattgctttc
    25321 tgtatacatt tgattgtagt cggatgaaat caatacaggc aatccggttt aaaatagggt
    25381 agaataaaat gtcttttcag acggcatcag ttcagccgtc aggacgcgga cttctaccct
    25441 ttgtttatat tttaaagaaa agagcgcacg ccatgatgga cgaaaaactc aatttctctt
    25501 acctgttcgg ttcgaacgca ccttacattg aggaattgta cgaggctttt ttggaaaacc
    25561 ccgatgcggt tgatgaaaaa tggaagcagt atttcaccga tttgagcaaa cagccgggga
    25621 cggttgctgt cgatgtcgca cacacaccga ttcgcgaatc atttgttact ttggcgaaaa
    25681 agaaaattgc atctgccgtt gcgggcggtg cggatgaggc aatgctgaaa aagcaagtca
    25741 gcgttttacg gctgatttct gcctatcgta tccaaggcgt gggtgcagcc caacttgatc
    25801 cgctcaaacg tatccccccg cgcgatattg aagccctcga tccgaaattc cacggtctgt
    25861 cagatgccga tatgacgctt caattcaata tgggcgaggg cgattttgcc aatcgcggca
    25921 aactgccttt gtcccaaatc atcagcaacc tcaaacaaac ctactgcggc cacatcgcat
    25981 tggaatatat ctatattccc aataccgaag agcgccgctg ggtacgcaat tattttgaaa
    26041 gcgtattgtc cacaccgcat tacaatgccg atcaaaaacg ccgtatcttg aaagagatga
    26101 ctgctgccga aactttggaa cgttatctgc ataccaaata tgtcggtcag aaacgtttcg
    26161 gtgtcgaagg cggcgaaagc gcgattgccg gtttgaacta cctgattcaa aacgccagta
    26221 aagacggcgt ggaagaagtc atcatcggta tggcgcaccg tggccgtctg aatgttttgg
    26281 tgaacatttt gggcaaaaaa cccggcgatt tgtttgccga atttgaaggt cgtgccgaaa
    26341 tcaaactgcc cagcggcgac gtgaaatacc atatgggctt cagctccgat attgctaccc
    26401 cgcacggccc gatgcatgtt tctttggcgt tcaacccgtc tcacttagaa atcgtcaacc
    26461 cggtggtgga aggttctgcg cgcgccaaac aaaaacgttt gggcgaaaac ggccgcgaca
    26521 aagtcttgcc ggtattgatt cacggcgact ccgcatttat cggtctggga gtcaaccaag
    26581 cgacattcaa cctgtctaaa acgcgcggtt ataccaccgg cggtacggtt catatcgtca
    26641 tcaacaacca aatcggcttt accacttccg atatccgcga tacccgttca accgtacact
    26701 gtaccgatat cgcaaaaatg gtttccgccc cggttatcca tgtgaacggc gatgatcccg
    26761 aacgcgtttg ctttgctatc caagccgctt tggattaccg caaaaaattc cataaagaca
    26821 tcgtgattga cgttgtctgc taccgtaaat ggggtcacaa cgagggcgat gatccgacct
    26881 tgacccaacc gatgatgtac aaaaaagtat cgcaacaccc gggtgcgcgt gctttgtaca
    26941 ccgagcaact gattgccgaa ggcgtgctaa cccaagccga ggcagacggt tacatccaag
    27001 cctaccgtga tgctttggac aaaggcgagc acgtcgagca aacaacgttg agcaacttcc
    27061 aacgcacgca aatcgactgg agcaaatacc aaggcaaaga ttggcgcgaa cacatcgaaa
    27121 ccggtttgcc tgccgcagat attgaacgtc tcactgagaa gtttaccgcc gtaccggaag
    27181 gctttgccct gcatccgact gcaaaacgtg tgattgaagc gcgtaaagcc atggcatccg
    27241 gcaaacaggc catagattgg ggtatggccg aaaccttggc atacgccagc ctcgtaacca
    27301 aaggtcacgg cgtgcgtatt tccggtgagg actcgggacg cggtacgttc tcgcaccgcc
    27361 acgccgtatt gcacgatcaa aaacgcgaaa aatgggacga cggtacttat gttcccctgc
    27421 gccatatggg cgaaggaatg ggcgagttcc tggttatcga ctccatcttg aacgaagagg
    27481 ccgtgatggc gttcgagtac ggctttgcct gctccgctcc tgacaagctc accatttggg
    27541 aagctcaatt cggcgatttc gccaacggcg cgcaagtgac gattgatcaa ttcctgtctt
    27601 caggcgaaac caaatggggt cgcttgtgcg gtctgaccac catcctgccg cacggttacg
    27661 acggtcaagg ccctgagcac tcttctgcac gcgtagaacg ttggttgcaa ctgtgttctg
    27721 agaacaatat gcaagtcatc atgccatctg aagcgtcgca aatgttccac ctcttgcaac
    27781 gtcaagtctt aggttcatac cgcaaaccgc tggtgatttt catgtccaaa cgcctgttgc
    27841 gcttcaaagg tgcaatgagc ccgctggaaa acttcaccga aggttcgact ttccgtccgg
    27901 ttatcggtga taccgccgaa cgcgcaagca acgacagcgt gaaacgcgtg gtattgtgtg
    27961 ccggtcaggt ttactatgac ttggaagccg gtcgagccga acgtaaactg gaagatgatg
    28021 ttgcgattgt tcgcgttgag cagctatatc cgttcccata cgacgaggtt aaagctgaac
    28081 tggcgaaata tccgaacgca aaatctgtgg tttgggcaca agaagagccg aaaaaccaag
    28141 gcgcgttcta ccaaatccgc caccgcatcg aagacgttat cagcgaagag caaaaactgt
    28201 cttatgccgg tcgtccaagc agcgcatcgc ctgcagtggg ctactcaagc aaacacattg
    28261 ctcaattgaa acaattggtt gaagacgctt tggcgttgta agcctagtag cattccgtct
    28321 gagtttgctc aggcggaatg cccatatgca gaattaaaaa cacacaacag gccgtctgaa
    28381 agggccattg gagacacaaa atgattattg atgtaaaagt acctatgctg tctgaaagcg
    28441 tatctgaagg cacgctcttg gaatggaaga aaaaagttgg cgaagcagtt gcccgtgacg
    28501 aaatcctgat cgatatcgaa acggacaaag tggttttgga agtaccttct ccacaagccg
    28561 gcgtattggt tgaaatcgta gcgcaagacg gtgaaaccgt tgttgccgac caagttttgg
    28621 cacgcatcga tacagctgct actgccgctg ctgaagcacc tgcagccgct cctgccgaag
    28681 cagccccggc cgccgctcct gccgccgcgc aaaacaacgc cgctatgcct gccgccgcca
    28741 aactggctgc tgagaccggt gttgacgtga acgcattgca aggttccggc cgtgacggtc
    28801 gcgtattgaa agaagacgta caaaatgccg ctgccaaacc tgccgcagcc actgctcctg
    28861 ctgttgcact tcctgcaggc gcacgtcctg aagaacgcgt accgatgagc cgcctgcgtg
    28921 cccgtgttgc agaacgtctc ttggcttctc aacaagaaaa cgccattctg actacattca
    28981 acgaagtcaa catgaaacca atcatggact tgcgtgcgaa gtacaaagaa aaattcgaga
    29041 aagaacacgg cgtgaaattg ggctttatgt ccttcttcgt taaagctgct gttgccgccc
    29101 tgaaaaaata cccggttgtg aatgcttctg tggacggcaa agacatcgtg taccacggct
    29161 acttcgacat cggtatcgcg attggcagcc cacgcggttt ggttgtacca atcctgcgtg
    29221 atgccgacca aatgagcatt gccgacattg agcaagcaat tgttgattac gcgaaaaaag
    29281 ccaaagacgg caaaatcgct atcgaagatc tgaccggcgg tacattcagt attaccaacg
    29341 gcggtacttt cggttctatg atgtccaccc cgatcatcaa cccacctcaa tctgcgattt
    29401 tgggtatgca cgccactaaa gagcgcgctg tggttgaaaa cggccaagtt gttgtccgtc
    29461 caatgatgta tctggctctg tcttacgacc accgtatcat cgacggccgc gaagctgtat
    29521 tgactttggt agccattaaa gacgcgttgg aagacccagc tcgcctgttg ttggatctgt
    29581 aatcgtttca gacggccttt tatttgttaa tgaaaaggcc gtctgaattt ttattaggaa
    29641 ataaaaatga aattggcagc tttaataacc ctatcatttt ttgttttaac tgcgtgcgat
    29701 aaagaagtaa ttattgtgca agaaaaatca aaagctgaaa aacaagcgga ttttgaagcc
    29761 caacaaaagg ctaactgtgc cgcgttgagc gacaaagtac gggcaaaaaa atcttattgc
    29821 aaaaaatatg agcagtaatt aaataaggta atcgggcgtt taaactgctc gattacctga
    29881 ttcatattag ttagatgtag cgtaatgtag tatcgtgcta caataggctc aacaaacgat
    29941 tgagaccgtc tgaaacattt gattcgaatg aatcggctgg tatggacttt cagacggcct
    30001 tttcttaaaa ccatcaaaac gcagtcattc aaaataaaaa agaaacgaaa agtatcgttt
    30061 ttattttgag atactgccaa aagcaaagga tgacacaatg tctcaatatg atgtagtagt
    30121 gattggtgca ggcccgggcg gatacgttgc cgccatccgt gccgcgcaac tgggtttcaa
    30181 aaccgcttgt gttgacgcag gcgttaacaa agcaggcaat gcccctgcat tgggcggtac
    30241 ttgcttgaac gtaggctgta tcccttctaa agccctgttg caatccagcg aacatttcca
    30301 cgctgcgcaa cacgattttg ccgaacacgg tattactgtc ggcgacgtaa aattcgacgc
    30361 ggccaaaatg attgagcgca aagatgccat cgtgaccaaa ctgaccggcg gagtgaaatt
    30421 cctgttccaa aaaaacaaag taaccagcct gttcggtact gcctcctttg ccggtaaaaa
    30481 tggcgatgct taccaaatcg aagtcgataa caaaggcgag aaaaccgtta tcgaagccaa
    30541 acacgtcatc gtagccaccg gttccgtacc gcgtccgctg ccacaagtcg ctatcgacaa
    30601 cgtgaacgtt ttggacaacg aaggcgcatt gaacctgact gaagtaccta ccaaactcgg
    30661 cgtgatcggt tccggcgtga ttggtttgga aatgggttcc gtatggaacc gcgtgggtgc
    30721 ggaagttacc attcttgaag ccgcgccgac cttcctggct gccgccgacc aacaaatcgc
    30781 caaagaagcc ttcaaatact ttaccaaaga gcaaggcttg agcattgaat tgggcgtgaa
    30841 aatcggcgac atcaaatctg aaggcgaagg cgtttccgtt gcttacgaaa ctgctgccgg
    30901 cgaagccaaa accgaagtat tcgacaaact gatcgttgct atcggccgta ttccaaacac
    30961 caaaggcctg aacgccgaag ctgtaggctt ggaaaaagac gagcgcggct ttatcaaagt
    31021 agatggcgaa tgccgtacca acctgcctaa cgtatgggca atcggcgacg tggttcgcgg
    31081 cccgatgttg gcacacaaag ccagcgacga aggcgttgcc gttgctgagc gcattgccgg
    31141 tcaaaaaccg catatcgact tcaacaacgt accattcgtg atttacaccg atcctgaaat
    31201 cgcttgggtg ggtaaaaccg aagagcagct caaagccgaa ggcgtggagt acaaaaaagg
    31261 tacttcaggt ttcggtgcaa acggtcgcgc attggccatg ggcaaagcca aaggtacggt
    31321 taaagtgttg gcagatgcca aaactgaccg catcttgggc gtacacatga ttggcccggt
    31381 tgtcagcgaa ttggttaccg aaggcgtgac tgcgctcgaa ttctttgcca gcagcgaaga
    31441 catcgcccgc atcatccatg cccacccaac cttgtccgaa gtggttcacg aagctgcatt
    31501 ggcggccgat aaacgcgctt tgcacggttg atagacatta aggcctccga caaattgaat
    31561 gttccgagag cttcgttttc tgatttataa ttccgtcaga caaacaaaca gcatttacat
    31621 tcattatgaa caaagaaata gtcggtattt tctttatacc ggcgggcatc atcagcatgt
    31681 gtatggccgc attgtggcag atgtatgtga tgatgaccga aacttatacg ctcaaccgtt
    31741 tcaaagataa agaattggtt tggcgcgtgg cattgttgtt tatcagtttc agccttgccg
    31801 tttatctgct ctgtccgaat tcgcgtaaaa aaggcatcgt cttttttatt ctcgggggag
    31861 gcggtgcagc catgtatctg ctggcgcgga tgtggttgcc tttcagcaag tgaaacgacg
    31921 attttccgac cgccgaaagg tagtctgaaa cgcacgggct tgccatttgg aggcaaactc
    31981 ggggcattcc actaatctaa aggagaaatc catgaattta cacgagtatc aggctaaaga
    32041 actgctggct agctacggtt tgcccgtaca aggcggtatt ttggcacaca acggcgaaga
    32101 agccgctgca gcttacgaca aattgggcgg caaattcgct gttgtcaaag cacaagtaca
    32161 cgccggcggc cgcggtaaag cgggcggcgt aaaagtcgtt aaaagccgcg aagaagctaa
    32221 agaagtggct gaaagcctga ttggcaccaa cttggtaact taccaaacgg atgccaacgg
    32281 ccaacctgtc aacagcgttt tggtttgcga agacatgtat ccggttcaaa ccgagctgta
    32341 cttgggcgca gtggttgacc gttctacccg ccgcattaca ttcatggctt cgaccgaagg
    32401 cggcgtggaa atcgaaaaag ttgctgccga aactcctgaa aaaatcttca aagtaaccgt
    32461 tgatccgctg gttggcctgc aaccttgcca agctcgcgaa gttgcgttcc aactgggctt
    32521 gaaagacaaa caaatcaacg agttcgtcaa actgatgacc ggcgcgtaca aagcgtttgt
    32581 cgacaacgac ttcgcactgt ttgaagtaaa cccattggca gttcgcgaaa acggcgcact
    32641 ggcttgcgta gacggcaaaa tcggtatcga cagcaacgcg ctctaccgcc tgccaaaaat
    32701 cgctgaattg cgcgacaaat ctcaagaaaa cgaacgtgag ctgaaagctt ctgaattcga
    32761 cctgaactat gttgccctgg aaggcaacat cggctgtatg gttaacggtg ccggtttggc
    32821 gatggccact atggacatca tcaaactcaa aggcggccaa cctgccaact tcttggacgt
    32881 tggtggcggc gctaccaaag accgcgtggt tgaagcgttc aaactgattc tggaagacaa
    32941 atccgttaaa ggcgtattga tcaacatctt cggcggtatc gtacgttgtg acatgattgc
    33001 ggaagctatt gtggcagccg ttaaagaaat caacgtcgac gttcctgtcg ttgttcgttt
    33061 ggaaggtaac aacgccgaac tcggcgcgaa aatcctgaac gaatcaggcc tgaaactgac
    33121 ttctgcagac ggcctgaatg acgcagccgg aaaaattgtt gcagccgtaa acgcctaagg
    33181 agaaaagaat gagcgtattg attaataaag acaccaaagt attggttcaa ggtttcaccg
    33241 gtaaaaacgg tactttccac tccgaacaag ctctggctta cggcactaaa gttgtcggcg
    33301 gcgttacccc gggcaaaggc ggtcaaaccc acctgaacct gcctgtattc aacaccatga
    33361 aagaagctgt taaagaaacc ggcgcggatg catccgtaat ttacgttccc gctccgtttg
    33421 tgttggattc tatcgttgaa gccgttgatt caggcgtagg cttggtcgtt gtgattaccg
    33481 aaggcgtacc tactttggac atgctcaaag ccaaacgtta tttggaaacc aacggcaacg
    33541 gtacccgttt ggtcggccct aactgcccgg gcgtgattac tccgggcgag tgcaaaatcg
    33601 gtattatgcc gggccacatc cacactccgg gccgcatcgg catcatttcc cgttctggta
    33661 cattgactta cgaagccgtg gcacaaacca ccaaactggg cttgggtcaa tcaacctgta
    33721 tcggtatcgg cggcgacccg attcctggta tgaaccaaat cgatgcactg aaactcttcc
    33781 aagaagaccc agatactgac gccatcatca tgattggtga aatcggcggt actgcggaag
    33841 aagaagcagc cgaatacatc caatccaacg taaccaaacc tgttgtaggt tacattgctg
    33901 gtgttaccgc tcctaaaggc aaacgtatgg gccacgccgg tgcgattatc tctggtggta
    33961 aaggtactgc cgaagaaaaa ttcgccgctt tcgaaaaagc cggtatcgct tacacccgca
    34021 gccctgccga gttgggcacg acgatgctgg aagtgttgaa ggcaaaaggt ttggcataat
    34081 caggtttgac aactgataaa catcaaatgc cgtctgaaac cggaaatcgg gtttcagacg
    34141 gcattttgtt tgtcatttca aaaagaggca gcctcaacat acccacatta ttttttgccc
    34201 tttgaggcag tcagaatatt tatcggtcaa acaggagcaa acccgtaagt caggttgcac
    34261 acgccttgtt tgaagtctgg gaatggataa gtatcgcatt tgaaaagact gatattattt
    34321 tttgcaggat tgttccggtt tctgtttcgg ctcgcgacat tttttgtttg ataggattgg
    34381 aacggatatg gtttgcacaa cgccgcaaat ccgtttcatc gcgcacggtg tgttcgtaaa
    34441 aacgccgatg ccaaataccg cgttcgtgcc gccgctgttt gcttgcagac aggttttggg
    34501 catgagggaa atgtgcggag aatttggttt taatcagccg ccggagtagg gaataatccg
    34561 catcatcggg cggcagcgtc caaatggcgt gaatgtggtt cggcagcacg gatacggcga
    34621 cggtttcaaa ggaatattgt ttttgcacat ccatataagc cgcacgcgaa gccgatacgt
    34681 tcgacaagca ggtgtgattt tggatcggcg agtttgacgg tgaaaaagaa tgtgccgccg
    34741 gcaatgaagt ttctgcgata acgcgccata ggatttttgt ttgtggtttg gatgttggag
    34801 aaggaatgat ttgttgttta agtttttaac tttgttgaag ttgaggtttt cagacgatat
    34861 cagaggtcgt ctgaaagcgc tagcttcaac gcagttaaaa gttgaaacgg caaaccgtca
    34921 atcaagcagc aaccgcgtgc gtaccgcata taccctacac gttggttttg aagttccgca
    34981 taattcacag gtagggtgtg tggcgcagcc acgcacgcgg tcggttggat atgcaggcta
    35041 cggcttgctt aattattcat cattagctaa tagatttaac tcaagcttat agtataaatc
    35101 gatatcttta agtaatttat ctatatcgca attgcacaat tcatttaacc aatcatttaa
    35161 ctcgttaata gttataatct cttcaatcgc atttttatat ctattaataa ttacctccat
    35221 accatttgat ttattattaa tatattcgat taattccgaa ttaacttgat cctttgatgt
    35281 ttctgtttta acaacaaatg taataggaaa gtgcaatact ttatctatat ccaacaaata
    35341 cttatcaaaa ttatttactt ccgttacctg gaagaatcta cccaatggct tcattacaaa
    35401 atcaattcca ccatcgttag catttgttct accagtttta tataatttta aaaactcttc
    35461 ttgtaaacta tctctagaat attcaaagaa tacagagata tttttgtaat gattctttaa
    35521 aatcgcatac gaaataattt caaaaattct tgcttctgat ttttcattta ataaaattga
    35581 aatttgtctt ttcttttcac tgacatcatg agattcaaat aaattttcaa ttgtttcgac
    35641 taaaaaacta tctttacact ttaatagata aatatatttt tctataatat caatagaaac
    35701 ttggcacaag tcaaaattat caacatataa atatttaata ttaattaaat attttccatt
    35761 gttgataatt attaattctt cgttaaactt atttttaaac tctccattta cacgactatt
    35821 tagagcgtga ttctgtaatt tatctcctcc atataatgac cgatagaaat aaaacaattt
    35881 gttatattca aaaccgtaga actttttgta ttcttctggt ttattgataa atttactttc
    35941 agcatagaaa tgcagtaagg aatatatggc atatatatta gccaaacttc tacgggtctt
    36001 agaattccca tagattgccc ccatttttga atccaaatac tgtaataatg ggcttttgtt
    36061 gtagatataa gagtaattat cagggtaata ctgtattaaa atttcttgaa caaattcttt
    36121 cattttaaac ctctattaaa tttaagtttt actatgatct ttttttgatt tattgctagt
    36181 ttttctcatc ttgtttttag ttaaaaattc cccattatac tcattagaaa tatgagttct
    36241 tctaatacca atcttaaaat aatctagatt aatttctata cctattgcct tcctttttag
    36301 tcgtactgcg acagaagaag tagtaaatga gccgctaaat ggatctaata tggtatcccc
    36361 tttattgctt gaagctaaaa taattctctc taataacttc tcgggttttt gtgtagggtg
    36421 attttcgtat tcatccattt tgtaacgaac tctattaaat tcccatacat tactaggtat
    36481 cttttttgca ttatagggct gcggcggagt ttttcgataa tctatcagtt ttctttcagc
    36541 tcctgtcttt gcctctatta aaatatcttc ggaattaaat gtatatttgc tgtttttgtg
    36601 atgtgtaaac attagaattg gctcgtaggt agagccaaat ttatttttag cttgaacacc
    36661 tgatgaatcg taagaccaaa caattctatt tataatgaaa tatttttcat ctacatacct
    36721 atctaaaata ggcatatatt gtacagaact cattaaatac attgtgccgt tgtcttttaa
    36781 aactctcata cattcatcaa tccacgaggc acaccaatct aaatattgat aagcatctgc
    36841 aaaaaagtct ttattattcc caaaatcttt accaattccg taaggaggat ctgcaaaaat
    36901 taaatctaca cttgaacttg gtaatgtttt tagaacttct aaacaatctc caagaataac
    36961 agtagagttt ttattaaact gtatttcaaa ttttttcatt ttcagccttt cagaatcata
    37021 cttaaatact ttccagtata actacttttc tcttccgcca cttcttcagg cgtccccacc
    37081 gcaataatcc tccctccgcc gtcgccgcct tctggcccta agtccacaat ccaatcggcg
    37141 gttttaatca catccagatt atgctcgata atcactatcg agttgccttt gcctttcaga
    37201 cggtctatga cttccagcag cagggcgatg tcggcgaagt gtaggccggt ggtgggttcg
    37261 tcgaggatgt agagcgttct gccggtgtcg cgtttggaga gttccaaggc gagtttgacg
    37321 cgctgggctt cgccgccgga gagggtggtg gcggattggc cgagacggat gtagcctaag
    37381 cctacgtcca tcaaggtttg cagtttgcgc gatacggtgg ggacggcgtc gaagaattcg
    37441 cgggcttctt cgacggtcat gtcgaggact tgactgatgt ttttgccttt gtattggatt
    37501 tcgagggttt cgcggttgta gcgtttgccg tggcagactt cgcaggggac gtacacgtcg
    37561 ggcaggaagt gcatttcgac tttaatcacg ccgtcgcctt ggcaggcttc gcagcggccg
    37621 cctttgacgt tgaaggagaa tctgccgacg ttgtagccgc gttcgcgcga gaggggtacg
    37681 ccggcgaaga gttcgcggat gggggtgaac aggccggtgt aggtggcggg gttggagcgc
    37741 ggggtgcgac cgatggggga ttggtcgacg ttgatgactt tgtcgaggtg ttcgaggccg
    37801 cggatgtcgt cgtatggggc gggttcttct tgggcgcggt tgagttcgcg ggcggtgatt
    37861 ttggcgaggg tgtcgttaat cagggtggac ttgccgctgc cggatacgcc ggtgatgcag
    37921 gtaattaaac cgagcggcag ttcgagggta acgtttttga ggttgttgcc gcgcgcgcct
    37981 ttgaggacga gcattcggcc gggattgacg ggcgtgcgtt cagacggcac ggcaatggat
    38041 tttttgccgc tgaggtattg tccggtaacg gagttttcac atttggcgac gttttcgggc
    38101 gtgtcggcaa tcaatacgtt gccgccgtgt tcgcctgcgc cggggcccat atcgaccacg
    38161 aaatcggctt cgcggatggc gtcttcgtcg tgttcgacca caatcacgct gttgcccaaa
    38221 tcgcgcagtc gtttgagggt ggccagcagg cggtcattgt cacgctggtg caggccgatg
    38281 gagggttcgt ccagtacata catcacgccg gtcaggccgc tgccgatttg gctggcgagg
    38341 cggatgcgct gggcttcgcc gccggagagg gtttcggcgg agcggcttaa attcaggtaa
    38401 tccagcccga cgttaatcag gaagccgagg cgttcggtga tttctttgag gattttttcg
    38461 gcgatttgtt ttttgttgcc gtctaaatcc agtgtttcaa agaattggtg ggttttggtg
    38521 agcggccagg cggaaacttc gtgcaacggc tcaccgctga cgtaaacgta gcgggcttct
    38581 ttgcgcaaac gtgcgccgcc gcagctcggg caggcgcggt ggttttggta ttcgcgcagt
    38641 ttttcgcgca cggtttcgct gtcggtttcg cggtagcggc gttcgagatt ggggatgatg
    38701 ccttcgaagg cgtggctgcg gttgaaggtg gtgccgcgtt cggacaggta agtgaaatca
    38761 atgacttcct tgcccgagcc gtgcaacacg acttttttga tttttgcagg tagggtttcc
    38821 caagcagcct gcacgtcgaa accgtaatgc cgcgccagcg actgaatcat ttggaaatag
    38881 aactggttgc gcttgtccca gccgtcaatc gcgcccgaag ccagcgacaa ttcgggatgc
    38941 gcgaccactt tttcggagtc gaagaaattg gtgttgccca agccgtcgca agtcgggcag
    39001 gagcccatcg ggttgttgaa cgaaaagagg cgcggctcca attcgggcag gctgtacgaa
    39061 catacggggc aggcgaaacg cgcggaaaac caatgctctt cgccgctgtc catttccatc
    39121 gccagcgcgc gctcgttgcc gtggcgcagc gcggtttcaa aactttccgc cagccgctgc
    39181 ttgatgttcg ccttcacttt cacgcggtcg atgaccacgt cgatattgtg tttgatgttt
    39241 ttttccagct tcggcacttc gtcaagctga tagacctcgc cgtccacgcg cacacgggca
    39301 aagccttgcg cctgcaagtc agcaaagaag tcgacaaact cgcccttacg ctcacgaact
    39361 gccggcgcaa gaatcatcac gcgcgtgtct tccggcagtt ttaatacggc atcgaccatc
    39421 tgagatacgg tttggctgga tagcggcaga ttgtgttcgg ggcaatacgg cgtgccgacg
    39481 cgtgcgtaca aaaggcgcag gtaatcgtgg atttccgtaa ccgtgccgac ggtggaacgc
    39541 ggattgtggc tggtggattt ctgctcgatg gaaatcgcgg gcgacagacc ctcgatcaaa
    39601 tcgacatcgg gtttgtccat catctgcaaa aactgccgcg cataggcgga caggctctcg
    39661 acataacgcc gctgtccttc ggcatacagc gtgtcgaacg ccagcgatga tttgccgctg
    39721 cccgataagc ctgttaccac tacgagcttg tggcgcggaa tgtccaaatc gacgtttttc
    39781 aaattatgcg tgcgcgcgcc gcgtatgcgg atggtgtcgt tgtcgtgcga atgttgggga
    39841 tgatggttgc acataatgga tgccgcctga aaaataaagg aaaactggta ttgtagcact
    39901 ttctcggatg ccgtctgaag ccgcgttcag acggcatttg tcggcggagt acggcagatt
    39961 ccgctataat gtcggcaatt ttaacccgct tgaacaaaag gatgacaaat gaaccgtctt
    40021 tacccccacc cgattatcgc ccgtgagggc tggccgatta ttggcggcgg tttggctttg
    40081 agcctgctgg tgtcgatgtg ttgcggctgg tggtctttgc cgttttgggt gtttaccgta
    40141 tttgccctgc agtttttccg cgaccctgcg cgtgagattc cgcaaaatcc tgaagcagtg
    40201 ttgagtccgg ttgacggccg tatcgtggtg gtcgagcgcg cacgcgatcc gtatcgtgat
    40261 gtcgatgctt tgaaaatcag tatttttatg aacgtgttca acgtgcattc gcaaaaatcg
    40321 cctgccgatt gtacggtaac gaaagtggtc tataacaaag gcaaattcgt gaatgcggat
    40381 ttggacaaag ccagcacgga aaacgaacgt aatgcggtgt tggcgactac ggcttccggt
    40441 cgtgaaatta cttttgttca agtggccggt ttggtggcgc gccgtatttt gtgctacacc
    40501 caagcaggtg cgaaactgtc tcgcggcgaa cgctatggct ttatccgttt tggctcgcgc
    40561 gtggatatgt atctgcctgt cgatgcgcag gcgcaagtgg cgattggcga taaagtaaac
    40621 ggtgtcagca ctgtattggc gcgtttgccg ctgactgcgc cgcaaatcga atccgagcct
    40681 gaatctgagc ctgctttaca aactgctccg gttgaaacag cggcaaaccc atctgccgaa
    40741 caacggcaaa tcgaggcagt ggcggctaag attcaggcgg ctgtgcaaga tgtgttgaaa
    40801 gattaatttt gcgaactgaa atagaaaata tcagtatcat cattcacacg aatgaggaag
    40861 tttggttttt tgaatttttg ctaatgttca caccgttatg ttcacgaaaa tgggaatccg
    40921 ggaacttaac gttacagcga tttatcggaa acggctgaaa ccgaacgaat t
//
LOCUS       DAWKQB010000002        38627 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.002, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000002 DAWKQB010000000
VERSION     DAWKQB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.002"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(143..233)
                     /locus_tag="WJL14_000040"
     tRNA            complement(143..233)
                     /locus_tag="WJL14_000040"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ser,seq:gga)
     gene            complement(272..358)
                     /locus_tag="WJL14_000041"
     tRNA            complement(272..358)
                     /locus_tag="WJL14_000041"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Leu,seq:cag)
     gene            complement(394..480)
                     /locus_tag="WJL14_000042"
     tRNA            complement(394..480)
                     /locus_tag="WJL14_000042"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(444..446),aa:Leu,seq:cag)
     gene            569..2944
                     /gene="rnr"
                     /locus_tag="WJL14_000043"
     CDS             569..2944
                     /gene="rnr"
                     /locus_tag="WJL14_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ7655176.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIEALVRELSIKEEEYEFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPHVERLCVVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIEHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQL
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPVPIKVKKRKGKS"
     gene            complement(3283..4746)
                     /gene="guaB"
                     /locus_tag="WJL14_000044"
     CDS             complement(3283..4746)
                     /gene="guaB"
                     /locus_tag="WJL14_000044"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ7655177.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVRWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4947..5027)
                     /locus_tag="WJL14_000045"
                     /pseudo
     CDS             complement(4947..5027)
                     /locus_tag="WJL14_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(5073..5534)
                     /locus_tag="WJL14_000046"
     CDS             complement(5073..5534)
                     /locus_tag="WJL14_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655178.1"
                     /translation="MNIIMDSTRKFGILWEENSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNAVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELCNIFSIDTTYMSG
                     GGNCEIDCLVLEMGYSGEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(5625..8183)
                     /gene="glnD"
                     /locus_tag="WJL14_000047"
     CDS             complement(5625..8183)
                     /gene="glnD"
                     /locus_tag="WJL14_000047"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ7655179.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLSGILTPMLRSRVSSAPMRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILFKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETQSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQTLLEQLS"
     gene            complement(8232..8552)
                     /locus_tag="WJL14_000048"
     CDS             complement(8232..8552)
                     /locus_tag="WJL14_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7655180.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8876..9349)
                     /gene="bfr"
                     /locus_tag="WJL14_000049"
     CDS             complement(8876..9349)
                     /gene="bfr"
                     /locus_tag="WJL14_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7655181.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(9377..9841)
                     /gene="bfr"
                     /locus_tag="WJL14_000050"
     CDS             complement(9377..9841)
                     /gene="bfr"
                     /locus_tag="WJL14_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ7655182.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="WJL14_000051"
     CDS             complement(10305..11288)
                     /gene="lipA"
                     /locus_tag="WJL14_000051"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ7655183.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="WJL14_000052"
     CDS             complement(11281..11904)
                     /gene="lipB"
                     /locus_tag="WJL14_000052"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ7655184.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLVGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11906..12181)
                     /locus_tag="WJL14_000053"
     CDS             complement(11906..12181)
                     /locus_tag="WJL14_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ7655185.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            12366..13436
                     /locus_tag="WJL14_000054"
     CDS             12366..13436
                     /locus_tag="WJL14_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ7655186.1"
                     /translation="MYRRKGRGIKPWMDAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(13633..14580)
                     /locus_tag="WJL14_000055"
     CDS             complement(13633..14580)
                     /locus_tag="WJL14_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ7655187.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSIVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(14595..15002)
                     /locus_tag="WJL14_000056"
     CDS             complement(14595..15002)
                     /locus_tag="WJL14_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ7655188.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHVGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            15083..15742
                     /gene="ung"
                     /locus_tag="WJL14_000057"
     CDS             15083..15742
                     /gene="ung"
                     /locus_tag="WJL14_000057"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ7655189.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNYLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            15739..15948
                     /locus_tag="WJL14_000058"
     CDS             15739..15948
                     /locus_tag="WJL14_000058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7655190.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            15845..16492
                     /locus_tag="WJL14_000059"
     CDS             15845..16492
                     /locus_tag="WJL14_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7655191.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVIDVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            16565..17038
                     /locus_tag="WJL14_000060"
     CDS             16565..17038
                     /locus_tag="WJL14_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ7655192.1"
                     /translation="MKTTGLRPSILPLPIRLIPPEKNGDYAFLLHLLKSLKPSGKGAI
                     ILPHGVLFRGNAEARIRTELLNLDLIKGIIGLPANLFYGTGIPACIIVIDKEHAQTAQ
                     FAEEGTNQVISGGSVFMIDASRGFIKDGNKNRLREQDIHKIIDTSTNLVTAVWCI"
     gene            17026..17340
                     /locus_tag="WJL14_000061"
     CDS             17026..17340
                     /locus_tag="WJL14_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEZ7655193.1"
                     /translation="MVHLSEIAAQDYNLNLPRYIDSGEVEDLQNLAAYLYGGIPAHDM
                     DALEAYWQVLGRMKNELFAEHDGRFTTIQRNRLQIFPTLNSLKSCGIGVEFRANLVQP
                     QI"
     gene            17382..18326
                     /locus_tag="WJL14_000062"
     CDS             17382..18326
                     /locus_tag="WJL14_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7655194.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAAIQPGRLIRKWSESLLD
                     AFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKESDEDANLTV
                     VFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSELERLSQEL
                     ENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQTLMTQEKA
                     AKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLENSIQQLIS
                     RLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
     gene            18468..18602
                     /locus_tag="WJL14_000063"
     CDS             18468..18602
                     /locus_tag="WJL14_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ7655195.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(18892..19407)
                     /locus_tag="WJL14_000064"
     CDS             complement(18892..19407)
                     /locus_tag="WJL14_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655196.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(19873..21792)
                     /locus_tag="WJL14_000065"
     CDS             complement(19873..21792)
                     /locus_tag="WJL14_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7655197.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYNPEHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRAE
                     KEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQEE
                     LERIETEIEKQFAER"
     gene            complement(21869..22264)
                     /locus_tag="WJL14_000066"
     CDS             complement(21869..22264)
                     /locus_tag="WJL14_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HEZ7655198.1"
                     /translation="MMRRRGKKMLFEENPIDGQFAEYECGAGGIRLTGQSFHKPVLVH
                     KDSVCLSQCRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGIS
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            22437..23744
                     /locus_tag="WJL14_000067"
     CDS             22437..23744
                     /locus_tag="WJL14_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ7655199.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQTCPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEKQNVIVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            23737..24171
                     /locus_tag="WJL14_000068"
     CDS             23737..24171
                     /locus_tag="WJL14_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655200.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQISSFGKLGAQEQLDLLGGANAFETRDKQCVADLKSE"
     gene            complement(24230..24499)
                     /locus_tag="WJL14_000069"
     CDS             complement(24230..24499)
                     /locus_tag="WJL14_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7655201.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(24681..27143)
                     /gene="lon"
                     /locus_tag="WJL14_000070"
     CDS             complement(24681..27143)
                     /gene="lon"
                     /locus_tag="WJL14_000070"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ7655202.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEADIKKAGMTKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELLVEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLASDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFSKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNFKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAVAESASKPKPRSRATKH"
     gene            27228..27419
                     /locus_tag="WJL14_000071"
     CDS             27228..27419
                     /locus_tag="WJL14_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ7655203.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            27394..28497
                     /locus_tag="WJL14_000072"
     CDS             27394..28497
                     /locus_tag="WJL14_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7655204.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKKVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(28569..30314)
                     /gene="recD"
                     /locus_tag="WJL14_000073"
     CDS             complement(28569..30314)
                     /gene="recD"
                     /locus_tag="WJL14_000073"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ7655205.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYASSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGKDENLPNIALAAPTGKAAAHMARALHRAINGFDALEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQG"
     gene            complement(30382..31077)
                     /gene="lolD"
                     /locus_tag="WJL14_000074"
     CDS             complement(30382..31077)
                     /gene="lolD"
                     /locus_tag="WJL14_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ7655206.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVLVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(31070..32317)
                     /locus_tag="WJL14_000075"
     CDS             complement(31070..32317)
                     /locus_tag="WJL14_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ7655207.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQIRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTAKLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            32523..32801
                     /locus_tag="WJL14_000076"
     CDS             32523..32801
                     /locus_tag="WJL14_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655208.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(32857..33474)
                     /gene="recR"
                     /locus_tag="WJL14_000077"
     CDS             complement(32857..33474)
                     /gene="recR"
                     /locus_tag="WJL14_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ7655209.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVDHCARCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(33541..35079)
                     /locus_tag="WJL14_000078"
     CDS             complement(33541..35079)
                     /locus_tag="WJL14_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ7655210.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     ASVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(35133..35507)
                     /locus_tag="WJL14_000079"
     CDS             complement(35133..35507)
                     /locus_tag="WJL14_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ7655211.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YASFSEETYDGIFNRQAPCRQG"
     gene            35657..37285
                     /locus_tag="WJL14_000080"
     CDS             35657..37285
                     /locus_tag="WJL14_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ7655212.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNATMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            37357..38610
                     /locus_tag="WJL14_000081"
     CDS             37357..38610
                     /locus_tag="WJL14_000081"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ7655213.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYKFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVRKVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWLALLEAANQADSGKIAAECRSQGKAHLIAEQI
                     DRARLAQIAPLQKAFRAAQDKTEKH"
BASE COUNT         8783 a         9930 c        10320 g         9594 t
ORIGIN      
        1 taccacaaaa cataccataa aaaaacggta acggatagtt gtaagcggtt gatgacgatt
       61 atagacaata acgggttttc ccaatgaaat tattgtttga ataataaaaa atcccaaacc
      121 gtaaaagttt gggatttgta tttggcagag aggaagggat tcgaaccctc gatacgctat
      181 tcacgtatac acgctttcca ggcgtgcgac ttaaaccact catccacctc tctaatggcg
      241 gaaattatcc caatcgggat aatttattat ttggtgcccg ggagaagact cgaacttcca
      301 cacccgtgag gataccagca cctgaagctg gcgcgtctac caattccgcc acctgggcaa
      361 tctaaatatt gaaataaagc aaagcatttg atttggtgcc cgggagaaga ctcgaacttc
      421 cacacccgtg aggatactag cacctgaagc tagcgcgtct accaattccg ccacccgggc
      481 tttgctttat ttgctgtttt gcggtacagt gtcctgctgc aaagaagtcg ctattatata
      541 gttcataaag agaatgtcaa cagtccaaat gaataaaaat attaaatctt taaatttacg
      601 ggaaaaagac ccgtttttaa gtcgtgaaaa acagcgttat gaacatcctt tgcccagtcg
      661 ggaatggata atcgagctgc ttgaacgtaa aggcgtacca tccaagattg aagctttggt
      721 acgcgaattg tcgattaagg aagaagagta cgaatttttc gaacgtcgtc tgaaggcgat
      781 ggcgcgggac ggtcaggttt taatcaaccg tcggggcgcg gtttgcgcgg cggacaaatt
      841 ggatttggtc aaatgccgtg tcgaggcgca caaagacggc ttcggtttcg ccgtgccgct
      901 cacgcccgcc aaagacggtg attttgtttt gtacgaacgc cagatgcgcg gcattatgca
      961 cggcgatatt gtcactgttc gtcctgccgg catggaccgt aggggccgcc gcgaagggac
     1021 ggttctggat attgtcgaac gcgcgcaaag caaagtggtc ggccgtttct atatggatag
     1081 gggcgtggcg attttggagc cggaagacaa gcgtctgaac caaagcatcg tattggaacc
     1141 ggacggcgtg gcgcgtttca aacctgaatc cggtcaggtc atcgtcggcg aaattgaggt
     1201 ttatcctgag caaaaccggc cggcagtggc aaaaatcatc gaagttttgg gcgattatgc
     1261 cgacagcggc atggagattg aaattgccgt gcgcaagcat catttgccgc accaattcag
     1321 tgaagcgtgt gccaaagccg cgaaaaaaat tcccgaccat gtacgcaaaa gcgatttgaa
     1381 aggccgcgtc gatttgcgcg acctgccttt ggtaacgata gacggcgaaa cggctcgaga
     1441 ttttgacgat gcggtgtttg ccgagaaaat cggacgcaat taccgtctgg tcgtggcgat
     1501 tgccgatgtc agccattatg tccgccccga tgacgctatc gacacggacg ctcaggaacg
     1561 cagcaccagt gtttacttcc cgcgccgcgt gattcccatg ttgccggaaa acctgtccaa
     1621 cggcatctgc tcgctcaatc ctcatgtcga gcgtttgtgt gtggtgtgcg atatggttat
     1681 cacttacgcg ggcaatatca aagaataccg cttctacccc gccgtgatgc gctctcatgc
     1741 ccgcctgacc tacaaccaag tttggaaatg gctttcaggc ggcatcgagc atccgttcaa
     1801 aacccaaatc gacacgcttt acaaactctt caaaatcctt cagaaaaagc gtttcgaacg
     1861 cggggcggtg gagtttgaca gcatcgaaac ccaaatgctt ttcgacgaca acggtaaaat
     1921 tgaaaaaatc gtccccgttg tccgcaacga tgcccacaag ctgattgaag aatgtatgtt
     1981 ggcggcaaac gtttgcgcag cggattttct gttgaaaaac aagcataccg cattgttccg
     2041 caaccatttg gggcccacgc ccgaaaaact cgccgccttg cgcgagcagc tcggtctgtt
     2101 ggggcttcaa cttggcggcg gcgacaaccc gtcgccgaaa gactatgccg cgcttgccgg
     2161 acagttcaaa ggcaggccgg atgccgaatt gctgcaagtc atgatgttgc gctccatgca
     2221 acaggcggtt tacgaaccgc attgcgacgg acactttggt cttgcctacg aagcatacgc
     2281 ccacttcacc tcgcccatcc gccgctatcc cgacctgacc gtacaccgcg ccatcaaagc
     2341 cgtgttgaat cagcaaacct acacgccaaa aaaaagctgg caggctttgg gcgtgcatac
     2401 ctcgttctgt gagcgccgtg ccgacgacgc cagccgcgac gtggaaaact ggctgaaaac
     2461 ctattatatg cgcgataagg tcggcgaagt attcgaaggt aaaatctccg gcatgaccag
     2521 ttttggtatc tttgtaacac tggacggcat ccacattgac ggcttggtgc atatcagcga
     2581 tttgggcgaa gactatttca acttccgccc cgaaatcatg gcaatcgaag gcgaacgcag
     2641 cggcatccgt ttcaacatgg gggacagggt tgccgtccgg gtcgcccgtg ccgatttgga
     2701 tgacggaaaa atcgattttg tcctgattgc cggggggagc ggcagggggc ggaaagttaa
     2761 atcatccgcg tctgccaaac cggcagggac ggcggggaaa gggaagccga aaaccgccgc
     2821 cgagaaaaaa acagcccgag gcggcaaagt aaggggaagg ggcgcgtctg ccgccgcaga
     2881 atcgaggaaa aaggcaaaga aaccggttcc gattaaggta aaaaaacgga aaggcaaatc
     2941 ataatgctga cggggcggct tggggagggc ggggcatact ttcccgaagc cgccgcccga
     3001 ttttttgata tggcggccgg cgcatcattt caggccaatc gaaacgcccg tattgaaaga
     3061 ttgcgtttat ttccaccgcc gttttaaagg ccggcggtat tcggcagacg gggcgcaaac
     3121 ggcgttcaga cggcattttc attctttcgg cgtgtccgtc cgaattgctt tgcccgtccg
     3181 cgcaatcagc ccgtgcggct tgccttgaac ggacaaaaaa tgccgtctga aacccgaaaa
     3241 tcaggtttca gacggcattt ttccttgaaa aggctgttca aatcagcggt ggtagttcgg
     3301 tgcttcttta gtgatttgaa cgtcgtgaac gtgcgattcg ctcatacctg cggaagtgat
     3361 ttccacaaat tcggcttttt cgtgcatttc ggcaatattg gcgcaaccca agtaacccat
     3421 actggaacgc agaccgccgg tcagctggtg gatgatgttc acaatcgggc ctttgtaagg
     3481 aacgcggcct tcgatgcctt cggggacgta tttgtctgcg ctgtcggttt tgtcttggaa
     3541 gtagcggtcg gcagaacctt ggctcatcgc gcccaaggaa cccataccgc gataggattt
     3601 gtacgagcgg ccttggtaga gttcgatttc gcccggtgct tcttccgtgc cggcaaacat
     3661 accgccgagc atgacgctgt acgcgcctgc ggcgagggct ttggcgatgt cgccggagaa
     3721 gcggatgccg ccgtcggcaa tcagcggaac gcccgtgcct ttgagggctt cggcaacgtt
     3781 gtgaatggcg gtcagttgcg gcacgccgac acctgccacg atacgggtgg tgcaaatcga
     3841 tcccggaccg ataccgactt tgacggcatc cgcgccggcg gcgaccaaat ccaaagcggc
     3901 tttggcagtg gcgatgttgc cgccgatgac ttggatgtgc ggataggttt ctttgaccca
     3961 acgcacgcgg tcgatcacgc cttggctgtg cccgtgggcg gtatcgacga caatcacgtc
     4021 cacgccggcc tcaaccaagg ctttgacgcg ctcttcggtg tcgccgccgg tgccgactgc
     4081 cgcaccgacg cgcagacggc cttcggagtc tttgttggca ttgggaaact cggtggtttt
     4141 taaaatatct ttgacggtaa tcagaccttt gagttcgtct ttttcgttca gaaccaaaac
     4201 gcgctcgact ttgtgcgtgt gcatcagttc gcgcgcttcg tctatgcttg tgccttcggg
     4261 gacggtaacc agacgttcgc gcggggtcat aatggcggaa acgggcaaat cgacgcggtt
     4321 ttcaaaacgc aggtcgcggt tggttacgat gccgacgact ttgccgtttt caacgacggg
     4381 caggccggac attttgcgtt tgcgctgcgc acgcatttcc aagacttcgc ggatgagcgt
     4441 tgtcggtgca acggttacgg ggtctttgac cacgccgctt tcgtggcgtt tcactttgga
     4501 aacggcgcgc gcctgcattt cgggcggcat gtttttatgg atgatgccga tgccgccttc
     4561 ttgtgccatc gaaatggcga ggcgcgcctc ggtaacagtg tccatcgcgg cggaaagcag
     4621 ggggaggttg agtgtgattt cgcgggtgag cttggtttga agtttaacgt ctcgcggcag
     4681 cacggtcgaa tgtgcgggaa ccaacaaaac atcgtcgaaa gtataggctt tttctacgat
     4741 acgcataatg ctcggtcttt cagtttgtgc aagatgcacg gcattatagc acgttaccgg
     4801 cggcttgaca gtttatcagg tttaattttg gtcccctttg aatagcccgg ttttcctttg
     4861 ccgaccactg ttgctcccgt tctttcaatt tcaggaaaag cttttttcta atttttggta
     4921 agtggctcag ttattgaagc cctatatcgg gcggtaactt ccagcacaaa aaaacggagt
     4981 agttctttat ttattttttc ctttaatttt cagtatatta tcttaatatt tcgagggtaa
     5041 catatctgct aatctagtta cagccccata tattatagat tcaattgaaa aataacagat
     5101 tcaactgtac ctttcttata cctgatttct ttaaagtttt tcccattgtc aaaactataa
     5161 aaaagtctct cttcctcacc agaatatccc atttctaata ctaaacaatc tatttcacaa
     5221 ttaccccctc cactcatata tgtagtatca atagaaaata tattgcataa ttccagagaa
     5281 ttatatttat ctatatcaaa tagttgctct ccaaaatcta aaccattgtt tccacctgca
     5341 taatacttct cttcaaagga tgacttcaaa ctattaaaaa ctgcatttag cgtaaaatat
     5401 ccatatgggc atatcttagg atatatattc tctcctatta ttatctgaat ttttccataa
     5461 ataaaaccat tacattcaga gttttcttcc cataaaatcc caaattttct tgttgagtcc
     5521 ataataatat tcatataaat ccttatatta ataaattatt tacaatatcc cccgctttca
     5581 gacggcatac ggcgtggcgg cggaatgccg tctgaaggcg ggcgttatga taattgttcc
     5641 agcagcgtct gcttcaatga ggactggatt tttgggtttt tcaggtctag gctaaaaacg
     5701 gtaaaactgt cttcggcgcg ttcatccagc gtggaaattt tggcgtagcg caggctgacg
     5761 ttgtgggcga aaaagacttc cgccatatcg gcgagcagga aagggcggtt gacggcggtg
     5821 atttcgacgg agtaccagtc ggggtagtct tcttcggggg tgatggtgat gctcggtgca
     5881 atgggcatat agcggctgcg gcggctgatg cggcggctgt ggctttgggt ttcggcaacg
     5941 gtgtgtccgt ggataaagct gttgagttcg gcttcgagcg cgctttggat gtcggggtag
     6001 tcttcggggg cgtgctgcga gggaatttgc acgatgaagg tgtcgaggat gtagtcgtgt
     6061 tcggtgataa aggcgcgggc ggcgagaatg tcgaagccgt ggcggctgaa gatacggcag
     6121 agacgggcaa acaagcgcgg cccgttgggc atgaaaacca taacttgaaa gctgtcgctt
     6181 ttgaacagga tgcggctgcg gacgatgggg gtttcaaaat cgtggactag gttggcggcg
     6241 tgccacagga tttcgcggga ctggtggcgg gcgaagtagg cggaaccgag cgcgttccat
     6301 agttttttct gctgtttttc ggggacggcg gcgcgggtga gtaagtcggc ggcttcctgc
     6361 cggcggcggc cgaagagggt gtgcgggttg ccgccgttgc ctgtaaggta gcgtccggcg
     6421 gcatggaaga ggctttccag caggctggcg cgccatgcgt tccacagctt gggattggtg
     6481 ccgcgtatgt cggaaatggt cagaaggtag agcgcgctga ggcgttcgtg ggtttggacg
     6541 cgtttgcaga aagcatcgag tacgtcgggg tcttggatgt cttctttttg ggcgacggca
     6601 gacatcagca ggtggttttc gaccagccag gcgagcaggt cgctttcttc tccggtcagg
     6661 aagtggtcag cggcaaattg gcgcgcgtct gcgatgcctt gtatggcatg gtcgccgccg
     6721 cgtcctttgg cgatgtcatg gaaaaaggcg gcaaggtaga ggatgtcttg tttttcaaag
     6781 gactgcatca gtgcagaggc gtagggcagc tcgtggctgt gcatatccaa ggcaaggcgg
     6841 cggacgttgc ggacgacggt gaggatgtgg tcgtccacgg gatagatgtg gaacaggtcg
     6901 tgttggagca ggccgacgat tttttcccac gcgggcaggt agcgtcccaa tacgccatag
     6961 aggttgagga agcgcagggt ttgggtcagt ccgttgccgt tgcggaaaaa gccggcgaag
     7021 cggtggcggt tttcagaatt ttggtagaag ctgcggttga ttttgcgcgt cgcgccccac
     7081 catgcgcgca gggtttgcgg ttcgagcgcg gtaatatcgt tgcgctgctg catgatttcg
     7141 acgattttga aaatgtgttc gggccgtctg aaaaaaatat cggtgtgccg cgcggcgatt
     7201 tggttgttga cttggatgta gtcgtcgtca atccgcaggg taacgcgcat cggcgcggag
     7261 gaaacgcggc ttcgcagcat aggcgtgagg atgccgctca gttgtttgac ggttttaatc
     7321 gcgcggtaaa acacgcgcat cagttcttcg ctttggcggc ggaggttcaa gccttcataa
     7381 cccatgcttt cggcgacttg cggctgcaaa tcgaacagca ggcggtcttc ggcgcgcttg
     7441 gcgtttaaat gcagatggat gcggatgtgg gcgaggcggc ggtagccgtg cgaaagcata
     7501 ccggcttcgg cacgcgtcaa aatccgctgt ttgagcaggt cgggcaggtc ggtcgccaag
     7561 ccttgcgcct tcgctatcca aagcagggtg tggatatcgc gcagaccgcc cggacagctt
     7621 ttgatattcg gctccaatac tgcccccgaa ccttgcgatt tggcgtggcg gtgttccatc
     7681 tccaccagtt ttgcctcgac aaacgccgcc acattgcgct gcgcgttcat tttttccgct
     7741 agtttatccg ccgtttggcg gttgccaaac aaaaacctag cctctaaaaa cgctgtgtcc
     7801 cccgtaatat cattgcgcac gctttcacat agttcatcaa cgctgccgct ttttacagac
     7861 ggcatcagtt tgcagtccca cagggtttgg acaaaccggg caatctgttc ctgaatgccg
     7921 tctgaaagcg gggcagggga gacaaccgcc aaatccacat ccgaacaggg atacagttct
     7981 ccgcgtccga agccgcctac cgccatcagg cataacgcgc tgttttgaaa atactctgcc
     8041 cacaatgccg ccagtaaggt ttcgactgcc gccgtgtatt ctctgaaaaa taccgacacg
     8101 cggttggctt tcaaataatg cgcttcggcg gcatcgcgct gctgtttgaa ggtttccagt
     8161 gctgaagaca ggtttgcagg catttttatt ctttcgattg gcgggaaaaa gggaggcgga
     8221 tggttcggcg gtcaaatacc gctttcagac ggcatttgtc gggtatcggc ggaattggtc
     8281 atcagcttca accgttcctg cggcggcagc agcaactgat acgccgccac gcccaaagcc
     8341 gccgccattt tttcgatatt cgacagggcg atgttccagc gtttgcgctc gactgccgac
     8401 acataagtcc tgtccaaacc gcattgccgc gccaattctt cttgcgacca acccttgttc
     8461 acgcggaaaa gccgcatatt gtatgccaat accgcccgca aatcctgttc gtcaggcagt
     8521 tcggcaggca gagtcaattt gttgcccatc atttgcttcc agataaatgg ttaaagttaa
     8581 gcatttgctg ttacggattt tacttcacat aaaagccaaa aaaaaggggt ggcggcagcc
     8641 acacccaaac acacacacat caagaggaaa gagataaaat cagacagatt ggaaaacagc
     8701 aaacaagcac cctgaatttc atatcggtat tatcaaacaa aaacccttat caggtattga
     8761 ttcaaatcaa tttagatttt attcgcaaac cgaaaaagaa aaacggcata tccgtttata
     8821 cggatatgcc gtctgatggt gcgggggact gctgtatttg gcagtagttt gtgttttagt
     8881 cctcttgcgc cgctgtttgc aggtagttgg gcagaccgat tttgccgata agctcttgct
     8941 gggtctccag ccagtctata tgttcttcgt tggtgtcttt ttgtttttcc aacaaatcgc
     9001 ggctgacgta atcctgttgc gcttctgctg tggcgatggc ggcaagcagg gcttcgtgtt
     9061 tttcctgttc tttggtcaaa tcgcaggcga tgatttcttc ggtggactcg ccaatcagaa
     9121 gcttgcccag ttcttgcagg ttcggcagac cttcgaggaa cagaatgcgc tcgatcaaat
     9181 cgtcggcagc tttcatttca acgatggact gtttgaagaa atgttcgccc agttcttcaa
     9241 agccccagtt tttcaaaata cgggcgtgaa ggaaatattg gttgatggtt accagcagca
     9301 agcctaagtt tttgttcagc tcgcggataa ccaaacggtc gcctttcata cggactcctt
     9361 ttattccgaa tacggattac agttggcttt ggtagtagtt gccttcgcca atcagctcga
     9421 tcaggcgcag ctgctgttcc agccagtggg cgtggtcttc ttcggtatct ttcagttggg
     9481 cgaccatcaa atcgcggctg acatagtctt gcgcctcttc gcacagtttg atgccttttt
     9541 tcaaagcgtc gcgcacttcg tattcggttt gcaggtcggc tttgaggcag gaaaccacgt
     9601 ccgtgccgat attcagctcg gcgcgcgcca ttttcggcgt accgcccagc atcaggatgc
     9661 ggcggatgaa gtcttcggcg tgtgtggttt cttcttccat ctcgtgattg agacgttcaa
     9721 aaagtttggt gtagccccat tcggagtaga ggcgggagtg gataaagtat tggtcgcgtg
     9781 ccgccagctc gccagacagc aattcgttca tataatcaac aacagcttga ttgccttgca
     9841 taatatctct ctttccgtaa cttggttttc ggtatgtctg aacagcatct gtgtcgctat
     9901 tcgggatgcg tgcattgtat gcaaaagctg tcggcatgac aaattttcta tttaaaatac
     9961 aaacaattat caaaagaaat agggcgtatt ccccgatgcc ttccaaatca gtatgctgtt
    10021 tcttatcggt tttttgtgct gttaaaacta agaatccata tcatccataa agaacattta
    10081 cacttgttaa ccatagcgaa aaacaaatag agcacggttt tttatcaaaa tttataatga
    10141 atcgttctca ttaaccgaca gattcttttg agattatcgg attttgggaa taaattatgc
    10201 cgtctgaggg gctttcagac ggcatagaca attaaagctg attaaacaac gcggcagaca
    10261 gcagcgtagg aaattatttt ttacttcgga acggtatttt gggtttaatg atgcccgcaa
    10321 ccgccgtggc tttcgcgtaa ggcttcggca gcctgttcgt cggcgtggta gctggaacgt
    10381 accatcgcgc cgatggcggc attgctgaag cccagttcgt atgcttcttt ttcaaagatt
    10441 ttgaactgct cgggcgtaac gtagcgcagg acgggcaggt gtccgtctga aggctggagg
    10501 tactgtccga tggtaatcat ttcgatattg tgcgcccgca tatcgcgcat aatttcacgc
    10561 acgtcttcgt ctgtttcgcc caagccgacc atgatgccgg atttggtcgg gatgtgcggc
    10621 atcatttctt tataacgttt taataagtct aaagaatgtt gataattggc accgggacgg
    10681 gcttttctgt acaggctcgg atgggtttct aggttgtggt tcatcacgtc gggcggggtt
    10741 tcggcaagga ttttgagtgc gatgtccaag cgtcctcgga agtcggggac gaggatttcg
    10801 attttggtgt tcgggctggt ttcgcggatg gctttgatgc agtcggcgaa atgctgtgcg
    10861 ccgccgtcgc gcaggtcgtc gcggtcgacg gaggtgatga cgacgtaacg caggttcatg
    10921 gctttgacgg attcggcgag gtttttcggt tcgtcggggt cgagcatatt cgggcggccg
    10981 tgtcccacgt cgcagaacgg gcagcggcgg gtgcagatgt cgcccataat catgaaggtc
    11041 gccgtgcctt tgctgaagca ttcgccgatg ttggggcagg aggcttcctc gcaaacggtg
    11101 tgcatttttt gttcgcgcaa aatgtctttg atttcaaaga atttgcgcga tggaagtttg
    11161 gcgcgtatcc attcgggctt ttttaatttt tcctgaaggg ggacgacttt gatggggatg
    11221 cgggcggttt tgtccgcgcc tctgagtttg atgccgcgtt tggggtcgtc ggttttgatt
    11281 tcactcattg ttgtctgctt tcggtgtgaa ttgtgtttca aggtgtgcgg taagtttggc
    11341 ggcgacttcg tccggcgtgg ggcagggttg gacaaaatcc gcgatttgcg tcatttccat
    11401 accggcgtag ccgcaggggt tgatgtgggt aaacgggctt aaatccatat tgacgttgag
    11461 cgcaagcccg tgatagacgg agccgttttt gatacgcagc cccagtgagg cgattttgcg
    11521 ttctccgaca taaacgccgg ggcgtttggg gtctgccgcc gcttcgatgc cgtattctgc
    11581 caatgtggcg atgatgctgt tttcaagcgc ggaaacgatg ttgcggacgc tggttttgcg
    11641 ccgtttgaaa tcaatcatcg tataaacgac caattgcccg ggcccgtgat aggtaatctg
    11701 cccgccccgg tcgatttgga cgacgggaat gtcgtcgcga atcagcaggt gttcgggttt
    11761 tcccaccagt ccttgtgtga acacgggcgg gtgttcgacg acccacagtt cgtcttcggt
    11821 gtcggcattc cgtccggcat taaaggtttt catcgcttca aaagtcggca gatattcgac
    11881 caaacctttg tgtatgattt tcatctcaaa gtaccacttt gaccagttcg tgcgaagtca
    11941 gcgcacggta gatgttgtcc aattgttctt ggttttcaac ctttacctgt acggtggcgc
    12001 cagtatagtt gcctttgctg ctcggacgcg tggtgatgtg gtgcgcctgc gtgtcggggg
    12061 cgtggaggcg gacggtgtcc aataccgcct gctcgaactc gggatgcacc gcgcccatta
    12121 ctttcaatgg gaaggtgcag ggaaattcga tgagggatgt tttgtttttt tgttcggtca
    12181 tgatgtgctg ccttgtcgtg tacggtatgc cgtctgaagg cgggtttgcc tttcagacgg
    12241 catcggatgt gcgttatttt agcctaaacc gcgataacag gctatcggga aggcggaggc
    12301 ttttttgacg gcacggcggt tctgctatac tggcgcacaa tattattttc ggaagggtgg
    12361 tttttatgta tcggaggaaa gggcggggca tcaagccgtg gatggatgcc ggtgcggcgt
    12421 ttgccgcctt ggtctggctg gttttcgcgc tcggcgatac tttgactccg tttgcggttg
    12481 cggcggtgct ggcgtatgta ttggaccctt tggtcgaatg gttgcagaaa aagggtttga
    12541 accgtgcatc cgcttcgatg tctgtgatgg tgttttcctt gattttgttg ttggcattat
    12601 tgttgattat tgtccctatg ctggtcgggc agttcaacaa tttggcatcg cgcctgcccc
    12661 aattaatcgg ttttatgcag aacacgctgc tgccgtggtt gaaaaataca atcggcggat
    12721 atgtggaaat cgatcaggca tctattattg cgtggcttca ggcgcatacg ggcgagttga
    12781 gcaacgcgct taaggcgtgg tttcccgttt tgatgaggca gggcggcaat attgtcagca
    12841 gtatcggcaa cctgctgctg cttcccttgc tgctttacta tttcctgctg gattggcagc
    12901 ggtggtcgtg cggcattgcc aaactggttc cgaggcgttt tgccggtgct tatacgcgca
    12961 ttacaggcaa tttgaacgag gtattgggcg aatttttgcg cgggcagctt ctggtgatgc
    13021 tgattatggg tttggtttac ggcttggggt tggtgctggt cgggctggat tcggggtttg
    13081 caatcggtat ggttgccggc attttggttt ttgttcccta tttgggtgcg tttacgggat
    13141 tgctgctggc aaccgtcgcc gccttgctcc agttcggctc gtggaacggc atcctatcgg
    13201 tttgggcggt ttttgccgta ggacagtttc tcgaaagttt tttcattacg ccgaaaatcg
    13261 tgggagaccg tatcgggctg tcgccgtttt gggttatctt ttcgctgatg gcgttcgggc
    13321 agctgatggg ctttgtcgga atgttggccg gattgccttt ggccgccgta accttggtct
    13381 tgcttcgcga gggcgtgcag aaatattttg ccggcagttt ttaccggggc aggtaggcgg
    13441 ttccgaaaca tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
    13501 caaatagggc aacgccgtac tggtttttgt taatccacta tatttgaagc ggaatacaac
    13561 cttgcccggg tttaaataaa aatgccgtct gaaccccgaa aacagagctt cagacggcat
    13621 tttcatcacg gcttatttgg cggttttgct gctgtcgata attttcatac cggcagaaat
    13681 caggctgccg atgtcggcaa cattggcggg cataatcagc gtattgcttt ctttggcaag
    13741 attgttgaac gcggcgacgt attgttccgc aatcttcaga ttgaccgcat ccgcgccgcc
    13801 ttgggtttga agggcggcgg caatttgacg gatggcttcg gcattggctt cggcaacaag
    13861 gcgcagggat tccgcttcgc ctttggcgcg gttgatgcgg gcgattttct cggcatttga
    13921 cgcattgacc gcagcctgag cctcgccttc ggattgttgg atttcggctt cgcgctgacc
    13981 gctggcaagg ttgatttgtt cgattttacg gccttcggat tcggcgatac gggcgcgttt
    14041 ttcgcgttcg gcggtaattt gcgcctgcat tgagcgaagg atttcttgag gcggcaccaa
    14101 gtctttaatt tcataacgca aaaccttcac accccaagct ccggcggctt catcgagggc
    14161 ggagacgacg atactgttga tttcgtcgcg ttcttcaaac gttttgtcca actccatacg
    14221 cccgataacg gaacgcagcg tcgtttgggc aagctgggta atcgccataa tgtagttgct
    14281 cgaaccgtat gaggcgagtt tggggtcggt tacttggaaa tagatgatac cgtcaacagt
    14341 cagctgcgta ttgtcgcgcg tgatgcagac ctggctgggt acgtctaaag ggatttcttt
    14401 cagcgaatgg cggtaggcga cgcggtcgat aaagggaatc aaaatattca aaccggccgt
    14461 cagggcgcga tggaaacgcc cgagcctttc gacaacgtgg acttcctgtt gtgggatgac
    14521 aacaaaggat ttgaaaccga aaacggcgac ggctaccaac aagataatga aaaattccat
    14581 aattcctccg agtgttaagg gtgtgtgata ataagaaggt tgccttcctt gcggacaatg
    14641 agggcgcgcg ttcccggttc aagctcttct tgccccgtat tttgcgcctg ccagtgcgta
    14701 ccgcgataaa aaacttcgta acggttgccg cctacgtgcc ggaggatttc ggcatattgc
    14761 ccggcatcca aatcctgata tgaatccgtt tcaacttttc ccacggcggt tttggcgtgt
    14821 acgaaccaaa tacccagcgc ggaaagcaga gcggcggtca agacggcggc aggcgtgctg
    14881 ccggtcagcc cgtaagcaat gcccgaaccc gccaaagccg cgctgacaac caaaagataa
    14941 accgttcccg tcaataattc gatgattaag acggcaacag cggcaacaaa ccatacagtc
    15001 atacatttcc ccacaaagcg cgtcgtttga caaaataacg caatatcagc agtatagccg
    15061 aatttgaaag gatagggcag atatggacac ttggcacgat gcactcggcg gcgaaaaaca
    15121 gcagccgtat tttcaggaaa ttttaaatgc agtcaggcag gaacgtttgt cgggacaaat
    15181 catctatccg ccggcggcgg atgtgttcaa cgcattccgc ctgacagcgt tcgaccgggt
    15241 caaagccgtc attctcggac aagacccgta tcacggggca gggcaggcgc acggtttggc
    15301 attttccgtc cggcagggta tccgcatacc cccgtcttta ctcaatatct acaaagaatt
    15361 ggaaaccgac atcgaaggct tttccatacc cgcgcacggc tgtctgacag cgtgggcgga
    15421 gcagggtgta ttgcttttaa acaccgtttt gacggtacgc gcgggacagg cgcattcgca
    15481 cgccctttta ggctgggaac gctttaccga taccgtcatc aggcagcttg cgacacaccg
    15541 caagcacctt gtcttcatgt tgtggggcgg gtatgcacaa caaaaaagga aactgataga
    15601 cagtcaaaat tatttgatac tgaccgcacc gcatccgtct cctctgtcgg catatcgcgg
    15661 ttttttcggc tgccgccatt tttcacaggc aaacagctat ttgagccggc acggtatcga
    15721 tccgataaac tggaagctgt gaatgccgat atagccgttg ccgccggcgt gttaaaatcg
    15781 cgtttgattt gtaatttcca tttattaggc aaaaccttat ggcaatcaaa aaaaaatcag
    15841 ctttatgcat cactttgggc gggctgcgac gagttgcgcg gcggtatgga tgcgagcctt
    15901 tataaagaac tatgtgctta cgctattgtt tttaaaatat gtttctgata agcataagta
    15961 cggcggcggc atgattgagc tgcacgccgg tacgactttt gacgacatcg tcaaactcaa
    16021 aaacaccgcc gacatcggcg accgcctgaa taagattatc gcccaaattg ccgaagccaa
    16081 cgacttaaaa ggcgtgatcg acgttaccga cttcaacgac gaagacaaac tgggtaaagg
    16141 taaggagatg atcgaccgtt tgagcaggct tgtcggcatt tttaaaaagc tcaacctttc
    16201 ttccaaccaa gccgaagacg acgatttgtt aggtgatgcc tacgaatacc tgatgcgcca
    16261 ttttgcgacc gagtcaggca aatccaaagg gcagttttac acgcctgccg aagtctcccg
    16321 cattatggcg aagattatcg gaatcagcgc agattgccgt ccagcaccag cgtttatgac
    16381 ccgacctgcg gctcgggttc gctgttgctc aaagccgccg cccaagccgg cagccaaatc
    16441 agcctttacg gtcaggaaaa agatgtggca accgcgtccc ttgcccgtat gaatatgatt
    16501 ttgcacaaca acgaaaccgc cgaaatcaac accgggaaca ccttgtccga ttcgtctttc
    16561 cgtgatgaaa acgacgggct taagaccttc gattttgccg ttgccaatcc gccttatccc
    16621 gcccgaaaaa aacggcgatt acgccttttt gctgcatctg ctcaaaagcc tgaaaccaag
    16681 cggcaaaggt gcgattattc ttccgcacgg tgtgctgttt cgcggcaatg ccgaagcgcg
    16741 tattcgcacg gaattgctta accttgacct tattaaaggc attatcgggc tgcctgccaa
    16801 cctgttttac ggcacgggca ttcctgcctg catcatcgtc atcgacaaag aacacgccca
    16861 aaccgcccaa tttgccgaag agggaacaaa ccaagttatc agtggcggca gcgtgtttat
    16921 gattgacgca tcgcgcggct tcattaaaga cggcaacaaa aaccgtctgc gtgagcaaga
    16981 cattcacaaa atcatcgaca cttccacaaa cctcgttaca gccgtatggt gcatttaagc
    17041 gaaatcgcag cacaagatta caaccttaat ctgcctcgct atatcgacag cggagaagtt
    17101 gaagacctgc aaaatctcgc cgcctatctt tatggcggca tacctgcgca cgatatggac
    17161 gcattggaag cctattggca agttttaggc cgtatgaaaa acgagttgtt tgccgaacac
    17221 gatggccgct ttaccactat acaacggaat cgattgcaaa tctttcccac tctcaacagc
    17281 ttaaaatcct gcgggattgg tgtggaattt agggctaatc tagtacagcc ccaaatttaa
    17341 tccactataa aatcgaaagc agccaaatca aagcccatat attggcgcac cccgattacg
    17401 ccgccttcaa agccggacac ctggcaaagt ttgccgcgtg gcacactcaa aacgaccttg
    17461 ccgccatcca accgggcagg cttatccgga aatggagcga aagcctgctg gacgcgttca
    17521 aacccggcag cctgattgaa gaatacgatt tctaccaaat cctgacggac tactgggcgg
    17581 aaaccctgca agacgatgtt tatctcatcg cccaagatgg ctggaaggcg gttaaaaacc
    17641 tggccgaaat caccaaagaa agcgatgaag acgcgaacct gaccgtcgtc tttgaggaaa
    17701 ccgaaaccgg caaaaaaggc aaagccaaaa ccaagcgcat cagcaaaaaa taccgcagcg
    17761 aagtcatcgc ccccgagctg gttgcccgcc gctacttttc agacggcatc gccaagctgg
    17821 aagaaaaaca aagcgagctg gaacgcctaa gccaagaatt ggaaaaccac atagaagaac
    17881 acggcggtga agagggtgcg ctgaacgacg tattggatgc aaaaggcaaa ctttccgcca
    17941 aacttctgaa aaccgcattg gaagaaagcg gcatagaaga aggcgaacgg gctgttttac
    18001 aaaccaccca aacactgatg acgcaggaaa aagccgcgaa agacgcagtc aaaacccaaa
    18061 tcgaagccct gaaccttgcc gtattcaaac aattcggccg actttccgaa gccgaaatca
    18121 agcagcttgc cgttcaagac aaatggcttg ccgatttaca aagccgaatc gaaaatcgct
    18181 tggaaaacag tattcagcag cttatcagcc gcttgaacac gctggaagac cgctaccgca
    18241 gcccgatggc cgagcttgcc cgagaagtgg aaaagtggca aagcaaagtc aatgcccacc
    18301 ttgaaaatat gggttttgga ggctgaaatg gcggcacaga caggctataa ggcgagcggg
    18361 ttttgagacc tttgcaaaat tccccaaaat cccctaaatt cccaccaaga catttagggg
    18421 atcgcggttc gggtgtccgc accgcttaat acgtcgtcgt ccacgaactg acccatttgc
    18481 tcgaacgcca tcgcaacgcc cgttttatgg cgcatatgga caactttctc ccaaactggc
    18541 aaagcatcaa acaacagctt aatgccttgg agttatttgc acaaatatat aatttaacat
    18601 aatatatatt atgcgaacta tcggaaacag ttgtacgtgt ccctgtggtc tttccaagta
    18661 ggaaaattaa agtatggaca atgcggctga atgtatagcc cggagcatcc gcgtatccga
    18721 aggtactgaa cgatatgccc tgcatccctt tcgacgcttc catccagccg gtattgggaa
    18781 atacgttgac cttcaatacg atatacaggc agccgacaca caggatatgc cgctgctttt
    18841 tcatcatttc ttctgtcaaa tccttggaac agttgatttg aaaacacggc tttacatacg
    18901 tcccaattct ctttgcagtg cttggatatt ttgctgcctg tccaatacat tgctttgcaa
    18961 tgcgttgatt tcttgatggt tgatgttgcc gccttttgcc agacgtgctt gtgataacat
    19021 tttttgggct tcaaccaatg ctttgcgttc gttgctcaat tctgtttcga gaatggagcg
    19081 tctgctgttg tttgagggtg cttgttgcgg cggcggcgta ttggattttg ccggcttgga
    19141 tactgttttg accggggctt tatatttgac agcctgtccg ccgtttgacg gtgatggtgt
    19201 cggttcggat gtttggggcg ggatatagcg ttcgctgctg tagttgccga ttgggggcaa
    19261 atcggttgag aggcagcttt tggacggctt ggtggtgtaa acggtttctc cgttgattgt
    19321 gcaggtgtag attttggccg cattcgcacc caatgggctt gaaatcaggg aaaagttgat
    19381 taggattaag gggagttttg atttcataat gtgcttgatg ctcggataat tatttgattt
    19441 tttggtattt tttgtaatgc tttcttggcg ttttacgggc ttttcttttc ggttggttgc
    19501 ttttgatttt ctttcggggc tttggcagcc tgcttgttct ttttcccggc aggttttttg
    19561 tttttctttt tactttctat gtgtattttg gcttcttaac tgagtttttt aattttcagg
    19621 cggtatccgc ctccctgatg gctgctgatt ttaggtaaat ccgcatcggc gcacgatcct
    19681 gctggggctg atgtatatac gcttttgcca tttgagttgc aatggtatac ggaggcttgc
    19741 gccgccgcgc cagaaaggga caggagaccc aaggcggcaa aaagtctgat gtttataccg
    19801 gtaaaaggcg gtgataacgc ccgaattata ccgttattgg caggcaaaga taagcaccct
    19861 gcccgcgctt ctttatcgct cggcaaactg tttctcgatt tcagtttcaa tccgttccaa
    19921 ttcttcttga caagccagcc aaacctcttc gatttgggca agttgtgttt tgacttttgc
    19981 cagctgggat aaggtatttt gcaatttttc tttgtttgct tcaaggtaag cgtcttcttg
    20041 tgctaaaaat gcttcacatg ccgtctgaat ttcggaaagc tgcgccattt ctttttcggc
    20101 acggtctatt ttctgctgta tcggcttgcc gcgtcgggct ttttcctgac ggatttgcgc
    20161 ttcgatgcgc ttggtgtctt tgcggctttg gctttgtgcg gatgctgctg gtgcggcggc
    20221 gttttcctgt gccaaacgcc attggcggta atcgttcaaa tcgccgtcga agttcttcag
    20281 acggcctttg tcgatcagga ggaagctgtc ggtagtcgct tcaagcaggc tgcggtcgtg
    20341 cgatacggcg attaatgcgc cttggaaact ttgcaacgcg agcgttaagg cgtggcgcat
    20401 atccaagtcc aaatggttgg tcggttcgtc aagcagcaac aggttcggct tttgccagat
    20461 aatcatggca agggcaaggc gggctttttc tccgccggaa aaaggttcgg ttttctgtaa
    20521 cgccatatcg ccaacaaaat tgaagccgcc gaggaaattt cggatttctt gttcgcgtac
    20581 ttcgggagaa agctgctgaa tatgccaaac agggttttgg tcggcgcgga tggtatcgag
    20641 ttggtgttgg gcaaaatagc cgatattgag tttttcggaa cggacgatgc tgccggagag
    20701 caaatcgatt ttgcccgcca aggctttgat aaatgtagat ttaccgctgc cgttgacacc
    20761 caataaacca tagcgcgcgc cgctttccag cgacagggta atgtcgtgca aaacagtttt
    20821 gccttcgtaa cccaaatctg cgtgttccag cttcagcaaa ggattgggca gatgctcggg
    20881 attgtaaaac tcaaaggaaa actcgctgtc cagatgcgcg ggcgcaacgc gttctagctt
    20941 tgccaaagcc ttcatgcggc tttgcgcttg aacggctttg gtggctttgg ctttgaagcg
    21001 gtcgataaag gattgcaaat gtttgatttg cgcctgctgt ttgacatagg cagcttgttg
    21061 ttgcgcgaga cgctgcgcgc gttcgttttg gtaaaaatcg taattgccgc cgtattgcgt
    21121 gagtttttgc tgcgataatt caatggtttg ggtagttgcc gcgttgagaa aatcgcggtc
    21181 gtgggaaatg atgatttgcg tgcagggtaa agaagcaaga tggttttcca gccacaagac
    21241 ggtttccaaa tccaagtggt tggtcggttc gtccaaaagc agcaaatcgg cgcggcaaat
    21301 cagggcttgg gcgagattga ggcgcatacg ccagccgccg gaaaaggatt tgacggggcg
    21361 gctgtgttct tcttgtgaaa aacccagccc gttcaacaat tttgccgcgc gggccggcgc
    21421 ggtataagcg tcgatttctt ccaatttggc atgatattcc gcctgcttca tgccgtcatt
    21481 ttgcgcttcc gcctgcctca atgccgtctg aaaagcctgc aactcggcat cgccctgcaa
    21541 aacgtaatcc aaagcggaaa tatccaaatc gggcgtttct tgggaaacgg aagcgagccg
    21601 ccagtttttc ggaatcgaga catcgccgcc gtcctgagtg attttaccct tgattaaggc
    21661 aaacaggctc gatttgcccg ttccgttttt gccgatcaaa ccgacgcgct gaccgggatt
    21721 gacggtagcg ttggctttgt cgagcaggac tttcaaaccg cgttgcaggg tgaggttttt
    21781 gatttcaatc ataacggaaa catcgtcggg cggaaaaagc ccgtatttta cctgaaagtc
    21841 agtgccgatg ccgtctgaaa tgggaaattt acggctgaag ccaagcccaa gccctgcgcc
    21901 cttccgagtg caggaaaacc aatgtcctga atgccgaatc ggtattcatg cattccacgc
    21961 tgattccgat tcgggaaaaa tccgccatga ttttgggatg gataaactcc tgagccgcgc
    22021 ccgtcccgat aatcaatatt tccggatagt caacaggttt gatgtcggac aacaggtttt
    22081 ccggagtcag atcggacaag gttcggcatt gcgacaggca gaccgaatcc ttatgtacaa
    22141 gcacgggttt atggaaactt tgccccgtca gccggattcc gcccgcaccg cattcatatt
    22201 ccgcaaactg tccgtctatc ggattttctt caaacaacat ttttttaccc cgtcgccgca
    22261 tcatctacac cgaaaggggt gcaaaatcag gcgaattcat gtaggattgg cagatttcat
    22321 ctgacccgcc tgccgatttc agacggcatt tgattcaaag tgcggcacaa ttatatcgac
    22381 agcggatatt ttcgtctttc aatatttaca tttcagtcgg cttacaagga gacacaatga
    22441 agccagtaaa catcggtctt ttaggtttgg gtacggtcgg cggcggtacg gctgccgtgt
    22501 tgcgggacaa cgcggaggaa atttcccgtc gcttggggcg cgaaatccgt atttctgccg
    22561 tgtgcgattt gagtgaagaa aaagcccgac aaacctgccc gtccgcagcc tttgtcaaag
    22621 atccgttcga actggtcgca cgtgaagacg tcgatgtcgt cgtcgaattg ttcggcggta
    22681 ccggcattgc caaagatgcg gtgttgaaag ccattgaaaa cggcaaacac atcgttaccg
    22741 ccaacaaaaa actgctcgcc gaatacggca acgaaatctt cccgctggcg gaaaaacaaa
    22801 acgtcatcgt ccaatttgaa gcggcagtag cgggcggtat cccaatcatc aaagccctgc
    22861 gcgaaggttt ggcggcaaac aggattaaat ccatcgccgg cattattaac ggcaccagca
    22921 acttcatcct ctccgaaatg cgcgaaaaag gcagcgcgtt tgccgatgta ctgaaagaag
    22981 cgcaggcatt gggttatgcc gaagccgatc cgaccttcga catcgaaggc aacgatgcgg
    23041 gccataaaat caccatcatg agcgcactgg cattcggcac gccgatgaac ttttccgcct
    23101 gctacctcga aggcatcagc aaactcgaca gccgcgacat caaatacgcc gaagaacttg
    23161 gctatcgcat caaactgttg ggcattaccc gcaaaaccgg caaaggcatc gagctgcgcg
    23221 tccaccctac cctgattccc gaaagccgcc tcttggcaaa cgtcaacggc gtgatgaacg
    23281 ccgtgcgcgt caacgccgat atggttggcg aaaccttata ttacggcgcg ggcgcgggcg
    23341 cattgccgac cgcttccgcc gtggttgccg atatcatcga catcgcccgc ctggttgaag
    23401 ccgataccgc ccaccgcgta ccgcatctgg cgttccaacc cgcgcaagtc caagcgcaaa
    23461 ccatcctgcc tatggacgaa attaccagca gctactacct gcgcgtccaa gccaaagacg
    23521 aaccgggcac gctggggcaa atcgccgcgc tgttggcaca agaaaacgtg tccatcgaag
    23581 cactgattca aaaaggcgtg attgatcaga ccactgccga aatcgtgatt ctgacccaca
    23641 gcacggtcga aaaacacatc aagtcggcaa tcgcagccat cgaagcactg gattgcgtgg
    23701 aaaaaccgat taccatgatc cgcatggaaa gcctgcatga ctgagccgaa acacgaaatg
    23761 ccgacggaag agcaggttgc cgcgcgcaaa aaagcaaaag ccaaaatccg caccatccgc
    23821 atttgggcat gggtcatttt ggcgttgctc gcttcaaccg ccctgctctc ccaatgcgcg
    23881 atgtccaaac cgcaggcaaa acagaaaatt gtcgagtctt gcgtgaagaa tattccgttt
    23941 gccgaaaaat ggcaaaacga tttgcgggcc cgcggtttag attcaaacaa tacccgcctt
    24001 accgtcgact actgcaaatg tatgtgggag cagcctttgg acagattgag cgagaaacag
    24061 attagttcct tcggcaaact cggcgcacaa gaacagcttg acctgctcgg cggcgcaaat
    24121 gcctttgaaa cgcgagacaa gcagtgtgtt gccgatttga aatcagaata atgtggaccg
    24181 ataaaaaagc cgattcttta aagaatcggc ttttttcata aaaaacggct tacagtgcgt
    24241 ctttcaatgc tttacctgca cggaatttag gcgttttggc ggcggcaatg gtcagaggct
    24301 cgccggtttt ggggttgcgg ccttggcgtt ccgcacgttc gcccacgtag aaagtaccga
    24361 aaccgaccaa agtaacggtg tcgccttgtt tcagggcggt ggttactgca ttggtagtgg
    24421 catccaaagc tttttgtgcg gcggctttgg aaatgtcggc ttcttgagca atcgcttcga
    24481 tcaattcaga cttgttcaca atcagtccct tcctgtctta aaaaatgatg aaatgcccga
    24541 atactcgggg tttgtactgc ttgagcaact ttcgctttat agcaattctg aaattgccgt
    24601 gtcaagcaaa aaatacggaa tcaccctatt tgacaggctt tcaggacgaa accgcatttt
    24661 tacaacacat ttcctgcgtt tcaatgtttg gttgccctgc tgcggggttt tggttttgaa
    24721 gcggattccg ccaccgcttc cgcaccagaa ggttctgccc aaggctcagg ctggctttcc
    24781 aaacccagag ccaatacctc gtctatccat ttgaccggat ggatggtcag gccggttttc
    24841 acgttttcag ggatttcttc caagtctttg aagttgtctt ttggaattaa aacgtgtttg
    24901 atgccgccgc gaagcgcagc caacagtttt tccttcagac cgccaatcgg caggacttcg
    24961 ccgcgcaggg taatttcgcc cgtcatcgct acatcggcgc gtaccgggat tttggaaaat
    25021 gcagacacca tcgccaaagt catggcaatg cccgcactcg gaccgtcctt cggcgttgca
    25081 ccttcgggaa catggacatg gatgtctttt ttctcgtaaa aatcagaagc caagcccact
    25141 gattccgcgc gggagcggac aaccgaccac gctgcagaca cggattcctt catcacatcg
    25201 cccaactggc cggtgcactg aatcacgccc ttacccggca atgctgcggc ttcgacggtc
    25261 agcaattcgc cgccgacttc tgtccacgcc agaccggtta cttgtccgat acggttttcg
    25321 ctttcggcaa cgccgtaatc gaagcggcgc acacccaaat agtcgtgcag atttttctca
    25381 tttactttaa ccgctttagg tttggctttg ctggttttct tggtttcaga caacctcttc
    25441 ttatcttcgt ccaaggtaat ttgcatcacc accttgcggc agattttggc aatctcgcgg
    25501 tcgagcgaac gcacgcccgc ctcgcgggtg tagtaacgga taatatcgcg caccgcgctt
    25561 tcttcgacga gcaattcgcc ttctttcacg ccgttgcgct tcatttgctt cggcacgagg
    25621 tactgcatcg cgatattgat tttttcgtct tcggtgtagc cggacagacg gatgatttcc
    25681 atacggtcga gcagcggggt cgggatattc aggctgtttg aagtcgcaat aaacatcaca
    25741 tcgctcaaat cgtaatccac ttcggcataa tgatcggcga acttgttgtt ttgctcggga
    25801 tcaagcactt cgagcaacgc gctggcggga tcgccccgga agtcgctacc caatttgtcg
    25861 atttcgtcga gcaggaacaa tgggtttttt acgccggctt ttgccatatt ctgcaaaatc
    25921 ttaccgggca tggagccgat ataggtgcgg cggtgtcccc ggatttcgct ttcgtcgcgc
    25981 acgccgccca aagccatgcg gacatatttc cgccccgttg ctttggcgat ggattcgccc
    26041 aaagaggttt tgcccacgcc tggagggccg accaggcaca gaatcgggcc tttgagtttg
    26101 tccatacgtt tttggacggc gaggtattcc aaaatccgtt ctttgacttt ttccaggccg
    26161 tagtggtcgg tatccagcac caatccggct ttggcgatgt ctttgctgac gcgggatttt
    26221 ttcttccacg gcagttcgag cagggtgtcg atgtagttgc gtacgacggt ggattcggca
    26281 gacatcggcg gcatcatttt gagctttttc agttcggaca ggcatttttc ttccgcttct
    26341 ttggtcatac ccgccttttt gatatctgct tccaaggcat ccagttcgcc gttttcgtct
    26401 tcttcgccca gttctttgtg tatcgcttta atctgttcgt tcagataata ttcgcgctgg
    26461 gatttttcca tttggcgttt gacgcgtccg cgtatgcgtt tttcggcctg cataatgtcg
    26521 agttcggatt ccagctgtgc cagcaggaat tccatccgtt tgccgatttc gggaatttcc
    26581 aaaatctgtt ggcgttgcgc cagtttcaac tgcaaatgcg ctgcgaccgt atcggttagc
    26641 cggctgtttt cggcaatgcc gttaatgctg ccgataattt cggcggggat ttttttattg
    26701 agtttggcgt attgttcaaa ctgcgccaac agggtgcggc gcacggcttc gaggtcggta
    26761 ttgccgcccg tgtcttcttc cacgaccgcc tctatatggg aaacgaacag cccgcccgtg
    26821 tcttcaatgg tcagaacacg tccgcgatac agcccttcga ccaatacttt taccgtgccg
    26881 tcgggtagtt tcaacacttg caggacttgt gcgaccgtac cggtctgata caggtcggcg
    26941 gcaaccggtt cttctaccgc cgcatcggtt tgcgccaaca ggaaaaccgg ctcctcgcgg
    27001 gtaatggcgt tttccagtgc ggcgatggat ttcggcctgc cgacaaacag cggcagaacc
    27061 atatgcgggt aaacgacgac atcccgcaaa ggaagggttg ccaaggcggc atattcctca
    27121 aaatgctttt ctttttgtgt cataggtact ctcttgtgtc tgacagattg ccgattttcg
    27181 cgtacattgg ggttgaaggt attatttcaa gcatatgtgg tttatttatg gagtttgatg
    27241 cgatgccgtc tgaaacattc cggcttcaga cggcatgggc ttggaaagac aaggcgggaa
    27301 caaaaaactg ttctgtgttg ccgctccttg ctgtaccatc cgtatggttt gcggttctgc
    27361 cgccctattt tcaaaacatg acgcaggtat tccatgtctt cttttatttt tcccgataac
    27421 gggttcgaat ggcgcaacga aagtctgcaa aagccgccca aaggttggca ttatgtccgc
    27481 gaaagtggtg caaacggcat tttgaaggct gcctatcaaa atattgcaac tgtctggcag
    27541 ggcgatttcc acaatgccaa acaggtgctt tccgcaatga agaagaaggt ccgtaaacct
    27601 gccgccgtcc gttccgatgc ggatatcgcc gcccttttcc acgcccaccg tatgaagcag
    27661 gcgcagcaga gccgtattct gaatatgctt gccgttgaaa tccgccccgg ttttgtgttg
    27721 gacaacaaac gcgcgcccga tatacgcgcc gccctgctcg acgtgtacgg agaggcggac
    27781 ggcaaaccgt ttttcctgcc gctcaatctg ctgctggggt ttatgggtgc gcacgagtgg
    27841 cataagaaag gggttgccgt tccgcagctg ggcggcagca tacacgttcc tttcggcgta
    27901 ttctcgccgt tgcgcggcga atacctcgac ctgctcgccc atgcgccgtc aacgggtttt
    27961 cagacggcat tcgatatcgg gacgggctcg ggcgtgcttg ccgccatttt ggcgaaacag
    28021 ggcattcctt ccgtcatcgg cacggatacc aatccgaaag ccgtcgcctg cgcccgtgcc
    28081 aatattgctc gtttgggctt tgaaaaacag gttgagatac gggaaaccga tctgtttccc
    28141 gaagggtttg ccgatctgat tgtctgcaat ccgccctggc ttcccgccaa gccgacttcc
    28201 gccgtcgaat ccgcgttata cgaccccgaa tctgcgatgc tggctgcgtt tttgcgggat
    28261 gcgccgaaac atctgaatcc cgacggagaa atccgcctga tcatttccga tcttgccgaa
    28321 catctgcacc tgcgtccgtc cgattttctg gataaggcat ttgctcaggc gggtttgcgc
    28381 gttgcggatg ttttgaaaac caaaccggtt cacaaaaaag ctgccgatcc gaacaatccg
    28441 ctggcttttg cccgaaacag ggaaactact ttcctgtacc gtttgaaaaa ggcataaggg
    28501 gcggcggtgc gcttccggcg gcaggaatct gaacgcccgc gccgccggca cggcatcatt
    28561 cgggcggatt atccttgtga aaatacccgc tcaagcatac tgcccaatgc cgtctgacgc
    28621 gttttgacag tggcggcagc ttgccggaag gtttcttctc cgccgaagaa tacgaacttc
    28681 tccctcgcgc gggtaatggc ggtatataac agctccttac tcaatccgga caatgcatcg
    28741 tccccttcgt ccgaaggtgc ggcggaaggc ggcagcagcc agacttcccg atattccgaa
    28801 ccttggcttt tgtggacggt catggcgaat gcgggttcaa attcgggcag gcagcttacc
    28861 gctacctttt taaatccgtc cgcatcggca aaataggcgg caaggctgcc ctgccgtccg
    28921 acatcttcca taatcagtcc gatgtcgccg ttgaacagtt caagcgcgta gtcgttctgc
    28981 ctgatcataa tcggctctcc ggcaaaatat gccaaatgtt ccggtatgtt cattttgcgg
    29041 cgtacatggc ggcaataggc ttcgttgaag tcttccgcat cctgccgcca agctgccaga
    29101 accacgatat ccgaaatgcc cgcgtatgcg gcttcgatat tgccgtcttt taccgcctgc
    29161 caataggctt tgtgtgcccg gtacaacctt tcgactcgag cgttcggact gcattccgaa
    29221 tgttccagtt cgtccggaaa ccggtcaaac aatgcccacg ccccttcatc gcccgatacg
    29281 gcggcacggg caaggcagcc gatgccgctg ttgtcgccga agcggtggct gaacgacaga
    29341 tgggcggtgt tttgcgccaa cacgggtgga tttgcgctga cgctgaaacc gtgttccgga
    29401 aggaagccgg ccagcctttg gtgcgtttct ccgtccaaaa cggttttttg tgacaaaacg
    29461 gacagcaccg cccctattcc gacggacggg agctggtttt catcccccag cagaatcacg
    29521 cgcgcgccgg ttttgaccgc ttttaaaagt tgcagcatca atgccgtatc cagcatagag
    29581 gcttcatcga taaccaatac gtcaaacggc agcgggtaaa cagggttgaa cgccgcctgc
    29641 attttgggcg ggcgcagctt cagcagtcgg tggacggttt gcccttccag tttgagcaaa
    29701 tggcggcgga cggcctccag cgcgtcaaaa ccgttgattg cacggtgcag cgcgcgcgcc
    29761 atatgtgccg ccgctttgcc cgtcggtgcg gcaagcgcga tgttgggaag attttcgtct
    29821 ttaccgcaaa tcagcgccag cagtttggca accgttgtcg ttttgcccgt tcccggcccg
    29881 ccggtaatca ccataaaaga ctgcaacagt gccaaggcgg cggcatcgcg ctgcccttcg
    29941 ctgcccgtgc cttgaaacca ttttgcgagg ttttgcctcg cgcctgccgc gtcgggggcg
    30001 gacgtgtcgg ctgccgccaa gcgttttatc tcggcagcca aatcgtattc caactgccac
    30061 atcctgccca aaaacagcct tctgccttcc aaaatcaaag gcgcggcgga tgttccgaca
    30121 acgggtgcga gtgccgacag cgcgtcagcc tcgccaccgc tcaaacggat aaacgaatga
    30181 ccgttttgca atgcctgaaa caggcgttcg gtgcagtttg caagcacttc gtcgccggaa
    30241 ctcgcatagt gttccaaaaa acggattgcc gcccttgccg ccgcttgggc aaattcatct
    30301 gtctgccgtt ccattttatt ccttatccaa atgccgtctg aaggcgtggg gcttcagacg
    30361 gcgcggtgtt gtttcggctg tttaggcgtt tgcgccctgt ttggggtgca ggctgccgtc
    30421 tttcatgacc atcacgcgct cgaagcggcc ggcgagttcg tcgtcgtgcg ttacgaccac
    30481 cagccccgtt cccaattctg ttttcagttc cagcatcata tccaaaacat tcctggcgtt
    30541 cgcacgatcg agattgccgg tcggttcgtc ggcaagcagg catttgggtt gggtaaccaa
    30601 ggcgcgtgca atggcggcac gctgccgctc accgcctgaa agttcgcccg cgcggtgcgt
    30661 cgaacggtgt ttcagtccga ccttttccag catcgccatt gccgcctccg cagcctcttc
    30721 acggcttttt ttgccgatca gaagcggcat catcacattt tccagtgccg aaaattcagg
    30781 cagaagatgg tggaactggt acacgaaacc gagatggcgg ttacgtaaat cgcccaaacg
    30841 ccgctggttt aaggtacgca aatcctcgcc catcagcagc accctgcctt cagacggcat
    30901 atccagcccg cctagaatat gcagcagcgt cgattttccg ctgcccgaag aaccgatgat
    30961 gccggtgctt tcccccgcgc ggatttccaa atccaagccg tgcagcacca gaacgtccaa
    31021 accgccgtcc cgatagcgtt tgcccacgcc ttcgcatttc aaaatcaact cactcataac
    31081 gcaaagcctc cgccggttgg gtttttgacg cgcgccggct cgggtagagc gtcgccacga
    31141 aagacagccc caaggaaatg caggcaatca gggcaacgtc gcccatatcg acatcgctgg
    31201 gcaggtagtc gataaaataa acctgcgaat tgatgaggtg gacaccgagc aggttttcaa
    31261 aaaacgccac gaccctgccg acgttccaac ccaaaagcac gccgcagacc acacccgcca
    31321 acgtgccgaa aaagcctgaa aacgcgccct gcaccataaa aatcttcatc acgccgccag
    31381 gggaaagacc caaagtccgt aaaatcgcaa tgtccgcctg cttttccgta accgccatca
    31441 ccaaggaaga gacaaggttg aacgccgcca cggcgataat cagcgtcagg atgatgaaca
    31501 tcatccgttt ttccaactcg accgcctcaa aatagctgcg gttgctgaac gtccaatcgc
    31561 gtacccaaac cgtatccctt tgcgcctccg gaatcagctt tgccgtcaag gcgggggcgt
    31621 tttgcgggtc ggcgagtttc agccgcagcc ccgcaacttc cttatccaag cggtacagta
    31681 cgcgcgcgtc ttggatatgc gtcatcgcca atgagttgtc aacctcgtaa acacccgttt
    31741 taaccagacc gaccacggta aactgtttca acctcggtac gactccggcg ggcgtaacat
    31801 tgccctccgg cgtgatgacg gtaactttat tgccgacttc cgcccccaaa gcctccgcca
    31861 agccgacacc gaggataatg tcaaactcgc ccggaatcag gtcttcaaat ttgcctgccg
    31921 gcattttgtc gccgtattcc accactttgc gctcttcaga cggcaaaatg ccgcgtatct
    31981 gcacgcccct gatttcgccc gcattggcca gcaatgcctg attggaaaca tagggcgcgg
    32041 cagccaaaat acctttgcgg ttttcggtaa accgaagcag gttgcgccaa tccgtatccg
    32101 tattgtcgat atagccgatt tcggcgtgcg gcgcgacatt caggagctgc ccgcgtattt
    32161 ctttctgaaa gccgttcata accgacaaga cgacaatcag cgcggttacg cccaaggcga
    32221 ttccggcaat cgaaaccatc gtgataaacg acataaagcc gttgcgcttt ttcgccctga
    32281 gatacctcaa gcctatccaa gcctctagag aaaacataac gctaccttaa aaatgtctgc
    32341 aaacgtgccg cccccggacg gcggtttggg ggcgacggca aaagtttatt gtaccgtaaa
    32401 acccaggcag cgtccgaacg cccgagtgcg ggggacgggg cggcagagcg ggcggaaaac
    32461 ttgcacaacg cgtcaaactg ccctatcctt tccttcagaa aaaccgtttc ttgaggaaaa
    32521 caatgaatat ccgaactgct tttgctttgt gcgccatcgc cttatccgcc gctgcggctg
    32581 cctacgccaa agaaatcaaa atcgatgcca acaacacgcc ttattccgaa gccgacgcgc
    32641 aaaagctggc ggcaacggca gtcggtatgg gtgttaagga acctatcagc ctgaacggcg
    32701 gcagcggcag cattaccgtg tccggcagca gcgcgacgca gtgcgtgttc aaagtcggca
    32761 acggaggcgc gttgcagatt caagggctaa actgcaagta aaccgcccgg aaaaatgccg
    32821 tctgaaggct tcagacggca ttttgcattg gcggcgttat gccccgcctt ctttaatcag
    32881 gcggcgttcg tacaccgcct gcgccagcgt tcccgcatcg acatattcca attcgccgcc
    32941 caagggaatg ccctgcgaca gcctgctgac tttgtaaggc aggtttttaa aaaactcgga
    33001 caggacatac gccgtcgcat tgccttctgc ggtaaaagcg gttgcaataa tgatttcttc
    33061 gacttctccg ccgcccagcc gttgcgccag cctgtccaat gcgatggcgg atacgtccat
    33121 tcccaatgcc gtattgattt gccccatcag gacgaaatac agcccgtcgt ggcagtttgc
    33181 cgcttccata ttcgacacgt cggcaggcat atgcaccacc atcagccgtc gcccgtcgcg
    33241 tgtttcatcg gcacaaatat cgcacaatcc gccttcgcaa aacgtgttgc acctcgcgca
    33301 atgatcgacc tgcttcaatg ccgtctgcaa ggcatccacc agtttttcag cctctttgcg
    33361 cttgtgttgg agcaaatgat acgctatccg ctgtgccgat ttcggcccga cgttgggtaa
    33421 aaccttcagc gcgtcgatca atccttggaa ggcatcttgt tttttgtggc tcatcatatt
    33481 ccgccgtatg ggaaaacggc cggaatattc cgaccgttat tttgtcaaca aaagtgtcaa
    33541 ttactgaccg tcgccgttgt cgaccgattg cgctcctttg gtctgtttga ttttgccgtt
    33601 gaaataacgt atcaacaagt cgaaagtatt ggcagactgc tgttgcgcca aagcctgttt
    33661 tgcaagcgga agctgtgcgg cgatatcatc cggcggggtt acagcctgta cttcgacaat
    33721 cacggatgcc ggcagaccga tcagcctgac gtaggcgggt ttgccgtttg ccggttttgc
    33781 tttcagcagt tccgcataag cctcgggcgg catggactgc cttgcctgct gtgcgcccaa
    33841 aacggacact tccgaccatt tcacgtcaac agccttgccg ccgttcagtt gggtaagcac
    33901 gtcttttgcc ttgttttcgg caagtttggc ggcttcggta cggatataag cctgacgtac
    33961 cgcgtctttg gcttcggcaa acggcagggt tttctcttcg cggacttctt tggcgcggac
    34021 gacccacgcg gtttcgctgt tgatggtcag cacttcggaa ttgtgttttt tcttcaatac
    34081 gtcgtcgctg aatacggcat tgatcaggtt ttcgggcata ccggacattt gcgcatcctg
    34141 cctgctcagc caagtttctt gggtttcgac tttcaaaccg ctgtttttgg cggcttcggc
    34201 aagcgaggaa ggatggttga acgcgtcatc gcccagcttt tcttttgcct tattgaagtc
    34261 ggcaaccgcc tttttcattt tcaattcgtt ttcgacggcg gctttttcct gctcgaaaga
    34321 aggtttggct tcatttgccg gcaaacgcgc cacgcgctct tcaaacgcat ttttcacttc
    34381 tgtttcgctg acggtctgtt tgtctgcaaa gtctttcaga ttcaaggcga catattccaa
    34441 tttgaccgct ttgggaagca ggtagtcttt tttgtttgcg ttataaaact tctgcaaatc
    34501 ggcttcagac actttgactt gggcgatgaa ttcgtcgggg ttgaaagtgt gcgaacggat
    34561 ggtgcggttg acctgcgtca gcctgatcag ctgttccgcc tgcgcgtcgc cgaccaatac
    34621 gccgttttgg acgaggttta ccaaattctg caaggcaaac tgatcgcgga tttcttcgac
    34681 aaactggtct tcagacatat gacgttggga aaggtagcgg tttaaaagcg cgtggtcgaa
    34741 tttgccgttt gcgtcgtgga aattgggatc gtccacgata atctgcttga tttgttcgga
    34801 agaaaccgaa atgcccatca gcttcgcgcc ctgtttcagg taggcgcgtt gtagcaggga
    34861 ttggaacacc gcgtcgcgcg aagggccgcc gccgtccgcc tgttcgttct gtatggcgtt
    34921 gttgatggag tggtcgctga ttttttcgtc gcccacttgg acgatgtagt cggcacccgg
    34981 atgggatacc gtgctgaccc cgaagccgac gaaggttaat gcaatcaggc ccaaaaggac
    35041 ttgggcgggc gttctgtatt tttcgatgga atggaacata ttttaaatcg ggatatagaa
    35101 tgggaaattc aagtcgggta ttgtaacggt ttttatccct gtctgcacgg ggcttgccgg
    35161 ttgaagatgc cgtcgtaggt ttcttcgctg aagctggcgt aaaccttgcc gccgcactcc
    35221 aatacgggac gcttgatcag gctcggcatt tcggacatca gtttgacggc ctccgccgtc
    35281 gaggacagca ctttttgctg tgtttcggca tcgagtttgc gccagcttgt cccgcgtttg
    35341 ttgagcaggg ttgccaaagg cacttgttcc agccacgagc aaatttccgc ttcagacggc
    35401 ctctgttttt taaaatcccg aaattcaaac tccaagccgt atccggcaag ccggtttttg
    35461 gcttttttga ccgtatcgca atttgggatg ccgtgaagga ttatcatttg gaaacctttt
    35521 gtctgaaata ataaaacgga tattttacta taagtgtctg aaaatttgcc cgtctgtttc
    35581 agacggcggg gcggttatgt tacaatccga aaattcgaaa aatttaatct cttgttcaat
    35641 aaaggcttta ccaatcatga tttctaccaa cggcatcacc atgcagttcg gcgcgaagcc
    35701 gctgtttgaa aacgtatccg ttaaattcgg cgaaggcaac cgctacggtt tgatcggcgc
    35761 gaacggctca ggcaaatcca ccttcatgaa aatcctcggc ggcgatttgg aacagacagc
    35821 cggcgaagtg gcgattgaaa acggcgtgcg tttgggtaaa ttgcgccaag accagtttgc
    35881 ctacgaagat atgcgcgtgc tggacgtggt aatgatgggt cacaccgaaa tgtgggcggc
    35941 gatgaccgaa cgtgatgcga tttacgccaa tcccgaagcc accgaagacg actacatgaa
    36001 agccgcagaa ctggaagcca agttcgccga atacgacggc tacaccgccg aagcgcgtgc
    36061 cgccgagttg ttgagcggcg tgggcatttc cgaagatttg cacaatgcaa ccatggcaga
    36121 agttgccccg ggcttcaaac tgcgcgtatt gctggcgcaa gccctgttct ccaagccgga
    36181 tgtattgctc ttggacgaac cgaccaataa cttggacatt aataccatcc gttggttgga
    36241 aggcgtgttg aaccagtacg actccacgat gattatcatc tcgcacgacc gccacttctt
    36301 gaacgaagtc tgcacgcata tggcagattt ggattacaac accatcacca tctatccggg
    36361 caactacgac gactatatgc tcgcctccgc ccaatcgcgc gaacgcgccc tgaaagacaa
    36421 tgccaaggcg aaggaaaaat tgcaggaatt gcaagaattt gtggcgcggt tcagtgccaa
    36481 caaatccaaa gcccgtcagg caaccagccg tctgaaacag gcagacaaaa tcaaatcgga
    36541 gatggtcgag gtcaaaccct ctacccgtca aaacccgtat atccgttttg aagccgatga
    36601 aaaagccaag ctgcaccgtc aggctgtgga agttgaaaaa ctggcgaaac gctttgaaac
    36661 ccagttgttt aaaaacctga actttatcct tgaagcggga caacgcctcg ccatcatcgg
    36721 cccgaacggc gcgggcaaat ccaccctgct gaaactcttg gccggcgcgt acaaccccga
    36781 atattcagac ggtctgctgc cggacgaagg cagcatcaaa tgggcggaaa aagccagcgt
    36841 cggctactat ccgcaagatc acgaaaacga cttcgatgtc gatatggatt tgagcgaatg
    36901 gatgcgccaa tgggggcagg acggcgacga cgaacaagtc atccgcggca ctttggggcg
    36961 tttgctcttc ggcagtaacg atgtcgtgaa aaaagtgaag gttctctccg gtggtgaaaa
    37021 aggccgtatg ctttacggca aactgttgct gttgaaaccc aatgtcttag tcatggatga
    37081 accgaccaac cacatggaca tggaaagcat cgaatccttg aacatggcgc tggaaaaata
    37141 caacggcacg ctgattttcg tctcccacga ccgtcagttc gtttcttccc tggctaccca
    37201 aatcattgaa ttggacggca aaggcggata tgaacactac ttgggcgatt acgaaagcta
    37261 tctcgagaaa aaaggcgtag cataaccgcc ggttggaaca atgccgtctg aagcggcttc
    37321 agacggcatt gttgataact ttaaaatagg aagcatatgc agacttatct cgtcggcggt
    37381 gccgtccgcg attatctttt gggcttgccc gtcaaagacc gcgattgggt ggtcgtcggc
    37441 gcagacgcac aaaccatgct ggcgcaaggc ttccagccag tcggcaaaga ctttcccgtg
    37501 tttctccatc ccgaaacaca cgaagaatac gccctcgccc gcaccgagcg caaaaccgcc
    37561 aaaggttacg tcggtttcag tttccacgcc gacaaagacg ttacgctgga gcaggatttg
    37621 atgcgccgcg acctgaccat caacgcgatg gcgcaagatg cggacggcaa gattatcgac
    37681 cctttcggcg gacaacggga tttggcggca ggcattttgc gccacgtttc cccagccttt
    37741 gccgaagacc ccgtccgcat cctgcgtact gcccgctttg ccgcgcgtta caagtttgaa
    37801 atcgccgaag aaaccataaa gctgatgcgg cagatggtgg aaaacggcga agcggacgca
    37861 ttggttgccg aacgcgtctg gcaggagttt gcgaaaggtt tgatggaaaa aaatccgcgc
    37921 aaaatgattg aagtgttgcg cgaatgcggc gcgctcaaag tcttgctgcc cgaagtcaat
    37981 gccctcttcg gcgtgccgca acgcgccgac taccatcccg aaatcgacag cggcatccat
    38041 accctgatga cgctgcaacg cgccgccgat atgggtttga gcctgcccga acgctatgcc
    38101 gccctgctgc acgacttggg caaagccaaa acaccgtccg acatcctgcc gcgccaccac
    38161 ggacacgacc tcgccggtgt cgaacccgtg cgcaaagtca atcaaaggct gcgtgcgccg
    38221 aaacattgtg ccgagcttgc cgaattggtt tgccgttggc acattatttt ccaccaagtc
    38281 ggacagctta aaagccaaac cattctgaac gtattgaaaa aaaccgacgc tttcagacga
    38341 cccgaacgct ttcagacggc attgaacgtc tgcattgccg acacgcaagg ccgtctgaac
    38401 cgcgaacaca cgccctaccc gcaacgcgcg cactggctcg ccttactcga agccgccaat
    38461 caggcggatt cgggcaaaat cgccgccgaa tgccgctcgc agggaaaagc gcaccttatc
    38521 gccgaacaaa tcgaccgggc gcggctggca caaatcgccc cattgcaaaa agcgtttcga
    38581 gcggcgcaag acaaaacaga aaaacattaa aacgtccaat gcagcca
//
LOCUS       DAWKQB010000003        34934 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.003, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000003 DAWKQB010000000
VERSION     DAWKQB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.003"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(278..580)
                     /locus_tag="WJL14_000082"
     CDS             complement(278..580)
                     /locus_tag="WJL14_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ7655214.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(552..839)
                     /locus_tag="WJL14_000083"
     CDS             complement(552..839)
                     /locus_tag="WJL14_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ7655215.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVRFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            919..1524
                     /locus_tag="WJL14_000084"
     CDS             919..1524
                     /locus_tag="WJL14_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655216.1"
                     /translation="MTIYFKNGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            1503..1799
                     /locus_tag="WJL14_000085"
     CDS             1503..1799
                     /locus_tag="WJL14_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ7655217.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKILYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            1796..2005
                     /locus_tag="WJL14_000086"
     CDS             1796..2005
                     /locus_tag="WJL14_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655218.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(2115..3356)
                     /locus_tag="WJL14_000087"
     CDS             complement(2115..3356)
                     /locus_tag="WJL14_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ7655219.1"
                     /translation="MQKWQTELYSTPLWLLQTLLIVSAASAVILFLTRKTRFGREFAY
                     ILRLCLTPKSAVKVLLLITAMVTLLLTEVRLNVLSTFMSKGLYDSMQDLNASAFWMFA
                     AMNAGVVLIRAFNNVVNDFLDQGLAIKWSERLNEVLTARWLADKNYYRLQMRRHAPDN
                     IDQRIQQDAQEFIASTIEFVRGMVNSVVTSLEFAVVLRGLAGILTVFGFDIPHGIVWF
                     VYIFVILATFIAMWIGNPLIRYNYENEKLNGDYRYSLIRVRDHAESVAFYNGEKHEYD
                     QLSDRFKAIIRNRWRIARQSVCLSGFNGMFTNGIKLFPIILQAPRLFAGQIKIGDIQQ
                     TVQAFARLQNALSFFRMFYNKFTAYRARLERLYGFLLSTEEQHSARQPEISEVSDGIA
                     LECKAAAAFARIGGSFCTAEA"
     gene            complement(3644..4291)
                     /locus_tag="WJL14_000088"
     CDS             complement(3644..4291)
                     /locus_tag="WJL14_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ7655220.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWCVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(4288..4656)
                     /locus_tag="WJL14_000089"
     CDS             complement(4288..4656)
                     /locus_tag="WJL14_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ7655221.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(4666..5337)
                     /locus_tag="WJL14_000090"
     CDS             complement(4666..5337)
                     /locus_tag="WJL14_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ7655222.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(5502..6221)
                     /locus_tag="WJL14_000091"
     CDS             complement(5502..6221)
                     /locus_tag="WJL14_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7655223.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALLPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASAESKGRSGEIVYL"
     gene            complement(6304..7617)
                     /locus_tag="WJL14_000092"
     CDS             complement(6304..7617)
                     /locus_tag="WJL14_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ7655224.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSATVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRTLPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(7696..8568)
                     /gene="hpnD"
                     /locus_tag="WJL14_000093"
     CDS             complement(7696..8568)
                     /gene="hpnD"
                     /locus_tag="WJL14_000093"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ7655225.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQKKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGEMPEHPVNQALQQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDSKTLEYADKMGLALQLT
                     NIIRDVGEDARNGRIYLPMEEMRRFDVPASVILQCRPTENFTELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            8739..10601
                     /gene="hscA"
                     /locus_tag="WJL14_000094"
     CDS             8739..10601
                     /gene="hscA"
                     /locus_tag="WJL14_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ7655226.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
     gene            10683..11339
                     /locus_tag="WJL14_000095"
     CDS             10683..11339
                     /locus_tag="WJL14_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7655227.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKN
                     ETAESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSK
                     IRLFKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR
                     "
     gene            11378..11905
                     /locus_tag="WJL14_000096"
     CDS             11378..11905
                     /locus_tag="WJL14_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HEZ7655228.1"
                     /translation="MGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIPA
                     LDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNKPGELTNVEQGESICVP
                     VDDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEKR
                     TSRKSGRASDVPELY"
     gene            11886..12137
                     /locus_tag="WJL14_000097"
     CDS             11886..12137
                     /locus_tag="WJL14_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEZ7655229.1"
                     /translation="MCRNCIDDFRQQLSQNSDYLREQDEDGYTPLHHEAIAGNALMVQ
                     AMLEYGANPASTTSEGYTALDFACLTGWQNVADLLEPRH"
     gene            12192..12533
                     /gene="fdx"
                     /locus_tag="WJL14_000098"
     CDS             12192..12533
                     /gene="fdx"
                     /locus_tag="WJL14_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ7655230.1"
                     /translation="MPKITVLPHTTLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(12820..13407)
                     /locus_tag="WJL14_000099"
     CDS             complement(12820..13407)
                     /locus_tag="WJL14_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ7655231.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGQENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            13620..13817
                     /gene="iscX"
                     /locus_tag="WJL14_000100"
     CDS             13620..13817
                     /gene="iscX"
                     /locus_tag="WJL14_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ7655232.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(13885..14286)
                     /locus_tag="WJL14_000101"
     CDS             complement(13885..14286)
                     /locus_tag="WJL14_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7655233.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            14497..15456
                     /locus_tag="WJL14_000102"
     CDS             14497..15456
                     /locus_tag="WJL14_000102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ7655234.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15524..16834
                     /gene="tilS"
                     /locus_tag="WJL14_000103"
     CDS             15524..16834
                     /gene="tilS"
                     /locus_tag="WJL14_000103"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ7655235.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPTVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCEEGSFDTVRWQAFSPRRKTHILRYFLKENGIPMP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRLEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAVSDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            16908..17693
                     /locus_tag="WJL14_000104"
     CDS             16908..17693
                     /locus_tag="WJL14_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7655236.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGRKPVGVYIPEGKMPSEEVRRLRAVVPEGKVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGSADVWSPKVLRAGM
                     GAHFLLDIYSQADLEIWLASYKGRVFATALREEKQAVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            17703..17900
                     /locus_tag="WJL14_000105"
     CDS             17703..17900
                     /locus_tag="WJL14_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655237.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            18162..18515
                     /locus_tag="WJL14_000106"
     CDS             18162..18515
                     /locus_tag="WJL14_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655238.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSALKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            18527..18832
                     /locus_tag="WJL14_000107"
     CDS             18527..18832
                     /locus_tag="WJL14_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655239.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            18894..20270
                     /gene="mpl"
                     /locus_tag="WJL14_000108"
     CDS             18894..20270
                     /gene="mpl"
                     /locus_tag="WJL14_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ7655240.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVYEGFDTAQLDEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRVGGARILAVLEPRSNTMKLGTMKAALPASLKEADQ
                     VFCYAGGADWDVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            20477..21529
                     /gene="bioB"
                     /locus_tag="WJL14_000109"
     CDS             20477..21529
                     /gene="bioB"
                     /locus_tag="WJL14_000109"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ7655241.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(21607..22242)
                     /locus_tag="WJL14_000110"
     CDS             complement(21607..22242)
                     /locus_tag="WJL14_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7655242.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     KKLIAKTKQKAEIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPNGNGLDVSPDGNITRR"
     gene            22565..22825
                     /locus_tag="WJL14_000111"
     CDS             22565..22825
                     /locus_tag="WJL14_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ7655243.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(22888..24747)
                     /gene="ilvD"
                     /locus_tag="WJL14_000112"
     CDS             complement(22888..24747)
                     /gene="ilvD"
                     /locus_tag="WJL14_000112"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ7655244.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNISNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTHAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIRLVISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(24920..26689)
                     /gene="cysI"
                     /locus_tag="WJL14_000113"
     CDS             complement(24920..26689)
                     /gene="cysI"
                     /locus_tag="WJL14_000113"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ7655245.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVPEGGKARIEQLARSYGLIHDGVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(26716..28530)
                     /locus_tag="WJL14_000114"
     CDS             complement(26716..28530)
                     /locus_tag="WJL14_000114"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ7655246.1"
                     /translation="MSEHDMQNTNPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPTAEPFSVTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKD
                     YKAKNIAGERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDKRFEELGAKRLLERVDADLDFAAAADGWTDNIAALLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDQATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLFFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(28527..29813)
                     /locus_tag="WJL14_000115"
     CDS             complement(28527..29813)
                     /locus_tag="WJL14_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ7655247.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLDCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(29852..30775)
                     /gene="cysD"
                     /locus_tag="WJL14_000116"
     CDS             complement(29852..30775)
                     /gene="cysD"
                     /locus_tag="WJL14_000116"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ7655248.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(30813..31547)
                     /locus_tag="WJL14_000117"
     CDS             complement(30813..31547)
                     /locus_tag="WJL14_000117"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ7655249.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(31563..33023)
                     /gene="cobA"
                     /locus_tag="WJL14_000118"
     CDS             complement(31563..33023)
                     /gene="cobA"
                     /locus_tag="WJL14_000118"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7655250.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGG
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
     gene            complement(33320..33976)
                     /locus_tag="WJL14_000119"
     CDS             complement(33320..33976)
                     /locus_tag="WJL14_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ7655251.1"
                     /translation="MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            34041..34931
                     /locus_tag="WJL14_000120"
     CDS             34041..34931
                     /locus_tag="WJL14_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ7655252.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPHVFKARAEDSHKGTFGTLAVV
                     GGAVGMSGAPVLAASAAMYLGCGKVWAGFNQETLPFAVIAGFPEIMLDTADGLTKRQG
                     INAWTAGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT         7672 a         9132 c        10069 g         8061 t
ORIGIN      
        1 aagcgggaat ccagacccca aacgcggcag gaatctatcg gaaacggctg aaaccgaacg
       61 gactggattc ccgctttcgc gggaatgacg gcggcagggg tttcgggatt cccgccttcg
      121 cgggaatgac ggaaagtggc gggaataacg aaaggcggga atgaccgcgc aaaaagccgc
      181 tgcccccttc ggacggcacc ggcaacaaaa aaccgcacgg ccgaaaccgc gcgggaaagg
      241 atagtcgggc gcgcccgata agcagcggcc gcccccgtta tttcaattgg gcgacatatt
      301 ggcgcaaaac ctcgttgatg cgcgtctgcc agcccttgcc gccggcgcgg aatttttcga
      361 ccacatcggc ggacaggcgt atggtaacga gttgtttcgg ggttttgcct gtgtttcgtt
      421 tttgcattac gcccttttct tccaattgtt tttgatggga aaaaagcacc tgtgccaagt
      481 cttcgggcag ggcttcggca atggggcggg caagcgcaaa atcttcggcg gcaagttccc
      541 gcacttcgcc gtcagcgttt gttaaggatt gacgttgcat attttttaac ctctctttta
      601 ttcgctttgc gaaaactgat gacacggatg ccgtctttta tcggcgtaaa acagacaatg
      661 tgcaggcgtt gcgtatcgcc tagataagca gcggcaacat aacgcggttc ggggtaatca
      721 aagcggacat cgggcacaat aacggccgtt gtccagcgta tttgcccgac tgattcaaag
      781 ggcaaattcc gctcttcgat attgcgttga tttttttcgg agtcaaattc aatcttcatt
      841 gcagcttgca gcgtattttg tcgttacatt ataagcggca aaaaaccaaa atgtaaatac
      901 aaaaaaggaa accccgaaat gaccatctat ttcaaaaacg gcttttacga cgacacattg
      961 ggcggcatcc ccgaaggcgc ggttgccgtc cgcgccgaag aatacgccgc ccttttggca
     1021 ggacaggcgc agggagggca gattgccgca gattccgacg gtcgccccgt tttaaccccg
     1081 ccgcgcccgt ctgaatacca cgaatgggac ggcaagaaat gggaaatcgg cgaagccgct
     1141 gccgccgccc gtttcgccga acaaaaaacc gccacggcat tccgcctcgc ggcaaaggcg
     1201 gacgaactca aaaacagcct cttggcgggc tatccccaag tggaaatcga cagcttttac
     1261 aggcaggaaa aagaagccct cgcgcggcag gcggacaaca acgccccgac cccgatgctg
     1321 gcgcaaatcg ccgccacaag gggcgtggaa ttggacgttt tgattgaaaa agttatcgaa
     1381 aaatccgccc gcctggctgt tgccgccggc gcgattatcg gaaagcgtca gcagctcgaa
     1441 gacaaattga acaccatcga aaccgcgccc ggattggacg cgctggaaaa ggaaatcgaa
     1501 gaatggacgc taaacatcgg ctgaaaaaat acgtttacca cctgttggta gccatcgacc
     1561 aactgttcaa cgccctaacc ggcggcgcgg cggacgaaac cctctcaagc cgcacctatc
     1621 gcggcgcgcg gctcgcccaa aagcccaaaa cccgctggaa gattttatat accctgatta
     1681 acggcgtgtt tttcgaccgc caacactgtc ggcaggcgta tatcagcgaa ctgaaaggca
     1741 ggcagcacga cgcgcggttc aaccaaagcc gcgccgccgg ggaaaagggg acgcgatgaa
     1801 ctacttcggc atggtagagt ttctgcgcct gatggcaatg gtgcggccgc catttgtctt
     1861 cttctccggc acccgcagcg aactgcctgc ttatcttgac ctcgtggcag aactgcgcct
     1921 gaccggatgg gagcgttttg caggcagcca aaccttgact gtgagcagca catcaacagc
     1981 aattcaagct acgacgacca cctgatttat aagttctgac cgcaagtagc gtactacttt
     2041 taaaggcata agataatccc cgtttaacag accattaaac ggggataaat ttgtgcaaaa
     2101 gctaatacaa tttcctacgc ttcggcggtg caaaagctgc cgccaattcg tgcaaaagct
     2161 gccgccgcct tacattccag tgcaatgccg tctgaaactt cgctaatctc gggttgtcgc
     2221 gcgctgtgtt gttcttcggt actcagcaaa aagccgtaca gacgctccaa tcgggcgcgg
     2281 taggcggtga atttgttgta gaacattcga aagaaagaaa gcgcgttttg cagtcgcgcg
     2341 aaggcttgga cggtttgctg gatgtcgccg attttgattt gtccggcaaa caggcgcgga
     2401 gcttgcaaaa taatcgggaa gagtttgatg ccgttggtga acataccgtt aaagccgctt
     2461 aagcagacgc tttgccgcgc aatgcgccaa cggttgcgga taatggcttt aaagcggtcg
     2521 gaaagctggt cgtattcatg tttttcgccg ttgtaaaacg ccacgctttc ggcgtggtcg
     2581 cgcacgcgga tgagggaata acggtagtcg ccgttgagtt tttcgttttc ataattgtaa
     2641 cgaatcaaag ggttgcctat ccacatggcg ataaaggtcg ccaaaatcac aaatatatag
     2701 acaaaccaaa cgatgccgtg cggaatgtcg aagccgaaca cggtcaggat gccagccaag
     2761 ccccgcaaaa caacggcaaa ttccagagaa gtaacgaccg aattgaccat gccgcgcaca
     2821 aattcgatgg tcgaagcgat aaattcctgc gcgtcctgtt ggatacgctg gtcgatgttg
     2881 tctggcgcgt ggcggcgcat ttgcaggcgg tagtagtttt tgtcggcaag ccagcgtgct
     2941 gtcagcactt cgttgagccg ctccgaccat ttaatcgcca agccttgatc gaggaagtcg
     3001 ttgacgacgt tgttaaacgc ccgtatcagc accacgcccg cgttcattgc ggcaaacatc
     3061 caaaatgccg aagcattcaa atcctgcatc gagtcgtaaa gccctttgga cataaaggta
     3121 ctcaacacat tcagccgtac ttcggttaac agcagcgtaa ccattgccgt aatcagcagc
     3181 aatactttga ccgcgctttt cggtgtcaga caaaggcgca ggatataggc aaactcccgc
     3241 ccgaagcgcg ttttccgtgt caaaaacaga atcaccgctg aagcggcaga aactatcaag
     3301 agagtctgta aaagccaaag cggtgtggaa taaagctcgg tttgccattt ttgcatggga
     3361 aatctcttac ggtatcaatg ccgtctgaaa agacgggtac agttgatttt ttgatgaagt
     3421 ttggggaagt tttgcaggtc agggcatatt gcgtgttaat ttaccataca accacgccgg
     3481 aattaagttt aaatttgaat aaaaggttcg ggttctgcaa aatacagaac ccgaaccttg
     3541 ttcggatatt gaaaccggcc gcccgatttt gggcggtgcg gcttgcaagt atcaagattc
     3601 gcatatgccg tctgaagctc ggagaggttc agacggcata tgcttatttg ggctgctctt
     3661 cgacgaatct cggacctttc aagatgccgt tgtgagaata gggcgacagc aggttgtatg
     3721 cggcggtttt ggaaacctga taaccgcggt cggtcaggct gttggcaatc tgattgacca
     3781 ctgcgctgac caaagccccc aacaggccgc tgttgctgtt gttgctgcct tcgcggatgc
     3841 tggccgaacc cgaccacaac tcttttccgt tgcgggaatc gaccagccgt gctttggcgg
     3901 atacggtcgt cacgctgtct aaaatttgat atgaagtgcc gtattcggta accgtaatgt
     3961 acaaaaccgc atcattgccg aaaatctgat gcagtttttc cggccggacg gcgtgaatat
     4021 cggcggcatt ggtcaagccg ttttgtttga aggtttcctc cacgactgcg gcggggaaga
     4081 cgtaatagcc ggcttcggaa agcggcgcgg cggtcgaagc cagtacacac catgttccgt
     4141 tgacatcggg cgattcgttc agcggcggaa ccaccaaaat tgaagccggt ttgctttcct
     4201 tgaatgacgt gtagtcgaaa tcgggcgctt tttgaacttg gcaggcagac agcgccaaca
     4261 cggcggcaag ccctaaaatc aaaggtttca tcgcttgcct cctttaccgg ttttcatcag
     4321 gaagtccata aatacgcccg attcgggaaa cagccttttc tcttcttcaa actggcggaa
     4381 cgcgccctct ttgtctcccg aacgggaaag cagcagtccc agatgggcgt gcgcacccgg
     4441 ggcggcattc atttttttgt tgccggcttc cacaaagtat ttttccatct tttcggtctg
     4501 cttgcccaac gaagtgtcgt cgtttttcaa accttcatag acggtatcgg gatagccgcc
     4561 gtaataatac agggattttt gcccgttgcc gccgcaggcg gtcagagcca agaccgccgc
     4621 acacagcgac aaacggctca aggttttcgg attcatcatt tctccttaac ggttgggttg
     4681 ccatgcgccg ttgtcaacag cctgaaccag gctgttgacg gcttcgcgga ttgccaagtc
     4741 taaaactttg ccgttcaaag tcgcatcgta gccggaagtg ccgccgaaac cgatgatttc
     4801 acggttggaa agtgcgtatt cgcccgcgcc ctgtgcggaa tagacgattt cggaagtatt
     4861 gacgttgacg atattcagag ccacttttgc ataggcgatt tgcgatttgc cgcgacccaa
     4921 aatgccgaag agctgatgat cgccgacatc tctgcgtccg aattcggtta catcgccggt
     4981 aacgacataa tctgcgcctt tcaggttatg cgctttgccg gaaatgccgg attcctgttt
     5041 taatgcgttc aaattggtgc ggttcagtac gttgaagcgg ttggtctgtt gcaggtgcgt
     5101 tactagaatg gtttttgcct ggctgcccaa acggtcttcc ccgtcggaga aaatgccttt
     5161 ttggaagctg gagcggttgt cgaatgttcc gacggaaatc ggggtacgaa caccgtgata
     5221 ttgcgtattg taggaggcga cttttgctac ctcgagactg cgtgaggatt cggtcgcaca
     5281 gccggtcagt gaaacggcag cggcggcaag gacaacggcg gtggaaacgg ttttcataaa
     5341 atttacccta aggtcaagtt aaggaaataa cgggttgtca ttattgtcct tatgtaaatt
     5401 taagtcaagg tgtttgtctg tgcgggacgg atgcgcacgg aaggtacgga tatttttcaa
     5461 acggaatcgc ggcgcgggcg ggatgcctgc cttttccgga tttagaggta aacgatttcg
     5521 ccgctccgcc ctttgctttc ggcacttgcc caccagacaa atgcgggcag cacgtccccg
     5581 tagcttttgc gttcgctttt ggcttcgccc ggatgggatt tgatgcgttg cggggaattg
     5641 atgggtccgg ggacgaggac gttggcgcgc aggttgccga agcgttccca ttcgtcggcg
     5701 gcgactttgc acaggtagtt caacgcggct ttggacgcgc cgaagccgcc ccagtaggct
     5761 ttgggggttt ccccgtggct ttcgccgacg aagataacgg atgcgtccgg cgactgtttc
     5821 agcaggggaa ggagggcgcg ggtcagcccc atcggggcga cggtgttgat gcggtattgg
     5881 ttgacccatt cggcgacggt ttggaaatcc agcggcgaga gcgcgtaaaa atagccggcg
     5941 cagtggacga tgccgtccag tttgccttgc gtggcttcgg caatggtggc ggcgaaatgt
     6001 tcaaattctt tttcttccgc gctaataagg tcaaagcaga tggcgaatgg ttcggggtat
     6061 ccggcttcga caatcgcgtc atacactttt tccagttttt tctgatgacg ggcaaccaaa
     6121 atcacggttg cgcctgccgc cgcataggct ttggcgacct gttcgcccag accttgcgat
     6181 gcgccggtta ctaagatggt tttgtcggac agtgtcgcca tactttttcc tttttggtgg
     6241 tcggttaagg tattttagcg ttttgccgca ccttgtaaag cgtcccgata ccggccggcg
     6301 ttttcagacg gcatcgctca ggctttgcag gcaggcttcc gccgacgcga aacctgattg
     6361 tacggcggct tcgagcgtgg cggggtagtc tgggtggagg tagtcgccgg cggggaagat
     6421 gcggtgccgg tgcaaccacg acaaatccgg cggcggggca tcggctgcgg ttgtggcgcg
     6481 tttttcggtg atgacgcgca cggcttcggg ttcgcccaaa tgcggaagga tgcgtttgag
     6541 gtcggcgtga actttgtccg cccacgcccg gtttgcaaac gcgccgacgc ggtcggaaac
     6601 gctgatgacg gcggacactt cgttttcagg cagtccgagc ctgccccggc aaagcagcca
     6661 ttgcaccgtg ccgtcggcaa ggccggtcag cggggcgggc aggcggacgg gttcggcgta
     6721 gcgcagatag acggtggtga tggcgtggta gcgaaggttt tgatatgccg tctgaacgtg
     6781 ttcgggcgtg ccttcgggca ggagcgcggc ggcgtggtag ggcgcggtgg cggggacggc
     6841 ggcatcgaaa gcttcgccgt tgacgagcac tttcccgtcc gggagggtgt tcagacggca
     6901 tacgcgcgtt tcgaggcgga tgtccgcgcc gagccgttga agctccgcca aggcgggttc
     6961 ggcgacgatt gcgcccaaat cctgcttggg taggagatag tcgctgccgg attttttcgt
     7021 cagcacgccg tcggacaaaa cgttgcacaa cacgcgcagg cttgcggttt ccaaaggcgt
     7081 gttgagcgca ccccacacca gcggctgcca aaactgcatc acggcggcac gcggcacgtt
     7141 ccgctgtttc agccattgtg caactgtcgt gtcgggctgt ccgaggcgtg cggacttctg
     7201 caaatcggac atatcggcaa gcagtttggc tttgaatgcg gacggtacgc gccgggcaag
     7261 cagcacgccg cccaaaatat gcagcggcgc gggcaggggg agggtgcgga actgcaaacc
     7321 gccgtgcata tgccagtgca gcggtacgcg caaaaaggcg gcacgggggt ctgaaccgat
     7381 ggttttcatc aggcgcaaca cgccccggta tgcgccgagc aaaatgtgct gcccgttgtc
     7441 caaaaaaccg aaaccgtcgg tatttccggc cagtgcgcgc gccctgccgc ccgcctgccg
     7501 gccggcttca aacagggtaa cgtcggcgtg ccgcgccaag gtgacggtgg cggacagtcc
     7561 tgcccagcct gcgccgatga cggcgatttt cgggcgcgga tgcggcgtgt tcatcattta
     7621 ttcctccaat ggttgaaacc ccgtttttcg gggcggcggg ggttgggttt tcctgaaggg
     7681 cgggcggtat gcgccttatg cccgttccgg cgtgccgggg cgcggtttga atccgaataa
     7741 ccaggttttc agggcaatgc gttttttgcg cggcgaaggg agggcgattt tgtatttgag
     7801 gacgttttgc gcgccgtctc ggtcgatttc gtttaaaagc gcgtaataaa ccgccgccat
     7861 aaccagtccg actttttggg cttttttgtc ggcatcaggc agcagcgata cggcttcacg
     7921 gtaggtttca cgcgcacgct ttatttggaa tgccatcaat tcggtgaaat tttctgtcgg
     7981 ccggcactgc aaaatcacac ttgcggggac atcaaaccgc cgcatttcct ccatcggcag
     8041 gtaaatccgc ccattacggg cgtcttcgcc gacatcgcgg atgatgttgg tcagttgcag
     8101 cgcaagtccc attttgtcgg cgtattccag tgttttgctg tctgaaaacc ccaaaatccg
     8161 cgcaatcagg cagccgacta cgcctgcgac gcggtggcaa tacagtttca attcttcaaa
     8221 actgccgtaa cgggcttgaa ccaaatccat ctgcatcccg tcgattaagg cttccagttc
     8281 atatttcggc agcttgaagg tttccttaac ctgctgcaag gcctgattga cggggtgttc
     8341 cggcatctcg ccaccgaaca ccttgtccaa atcgccgcgc caccagttca atgttgcctg
     8401 tgcaacatcg gggttggaac attcgtcaac cacatcgtcc aattcgcggc aaaaagcata
     8461 taaaaccgtt accgcatccc gttttttctg agtcaggaaa cggaagcccg ataaaaaact
     8521 ggagcggctt tcttctgctt tttgacggca atagtcgagt cctttcacga tttatattcc
     8581 taatgatggg cgagaaagac ggattttatc ggcatttggc ggtagagggc aatttcggcg
     8641 gcacggccca atccttagcg gtttgctcaa ctatcggcgc aaattctgtt aaaatgccga
     8701 cttccttcct ttacacaccg caccgacagg cagaatttat ggctcttttg cagatttcag
     8761 aaccgggtat gtccgccgcc ccgcaccgac accgtttggc ggcaggcatc gatttgggta
     8821 cgaccaacag cttggttgcc accgtccgca gcggcagtgc cgcctgcctg cccgatgccg
     8881 aagggcgcgt taccctgcct tccgtcgtcc gctatctgga aaacggcggc attgaagtcg
     8941 gcaaaaccgc cctgtccgcc caaaaaaccg acccgctgaa caccgttagc tccgccaaac
     9001 gccttatcgg gcggacgctt gccgatctgc atcaaaatac gcattacctg ccttaccgtt
     9061 tcggcgacaa tcaacgcgtt atcgaactgc atacgcggca gggggtgaaa acgcctgtcg
     9121 aagtgtcggc ggaaatcctc aaaaccctta aatcgcgcgc cgaagaaacc ttgggcggcg
     9181 atttggtcgg cgtggtgatt accgtccctg cctatttcga tgatgcccaa cgccaagcca
     9241 ccaaagatgc cgcgcgtctg gcgggtttga acgtattgcg cctgctcaac gaacccaccg
     9301 ctgccgcaat cgcctacggg ctggacaacg cctcggaagg cacgtttgtc gtgtacgact
     9361 tagggggcgg cacattcgac gtatccgtat tgcaactgac caaaggactg tttgaagtca
     9421 aagccaccgg cggcaacagc gcgttgggcg gcgacgattt cgaccaccgc ctgttctgcc
     9481 gcctgctcga acaaaacgga ctctcccaac tcaacgaaca agacagccaa ctcctgctct
     9541 cgctcgtccg cgccgccaaa gaacaattaa ccacgcaaac cgaagcgcgc attcaggcga
     9601 cgctttcaga cggcatggca atcgacacaa gcatcagtcg cgccgagttc cacaacctga
     9661 cgcagcattt ggtgatgaaa acgctcgaac cggtcacaca ggcgttgaaa gatgccggtg
     9721 tcggtaaaaa cgaagtcaaa ggcgtgatta tggtcggcgg ttcgacccgt atgctgcacg
     9781 tccaacaggc agtcgccacc ttcttcggac aaaccccgct gaacaacctc aatcccgacg
     9841 aagtcgtcgc gctcggcgcc gccatacagg caaacgtcct cgcaggcaac aaaaccgacg
     9901 gcgaatggct gctgctggac gttacacctt tgtcgctcgg tttggaaacc tacggcggct
     9961 tggcggaaaa aatcatcccg cgcaattcca ccatccccac cgcgcgcgcg caggacttta
    10021 ccaccttcaa agacggtcaa accgcgatga cgatacacgt cgtgcaaggc gagcgcgagc
    10081 tcgtttctga ctgccgcagc cttgccaaat tcaccctgcg cggcattcca ccgatggcgg
    10141 cgggtgcggc gcgtatccgc gtaaccttcc aaatcgatgc ggacgggctg ctgtccgttt
    10201 ccgcccaaga acaaagcacc ggcgtacagg cgcaaatcga agtcaaacct tcctacggct
    10261 tggacgacgg cgcgattacc cgaatgctta aagacagtat ggacaacgcc gccgaagata
    10321 tggcggcacg cgcccgcgcc gaagccgtag tcgaagccga aagcctgacc gatgccgtca
    10381 acgccgccct cgagttggac agcgatttac tggatgccaa agaattgcaa cagatccggc
    10441 aaggcatcgc cgatttgcaa ggccgtctga aagacggaaa agccgaagac atccgctccg
    10501 ccgtcgccaa actcagccgc agcaccgaca acttcgccgc caaacgcatg aaccgcaata
    10561 tccaacgcgc gttgactggt caaagcgttg ataatatttg acggcaaatg gtttcagacg
    10621 gcattggata aagaggtcgt ctgaaaaaat atcatctcac agaaataaaa taaaaaacaa
    10681 caatggaaaa gtcaggcatt gtctatctga tgaaaaccgt catcaagggc gtgtataaaa
    10741 tcggcatttc ggatgtaagc aattttgaag gcagaatgcg ccatttggaa aacaacggtt
    10801 atgcgaacgt tgccgggttg gaacgcatcc tcgccgtcaa aaccgacaat tacaaagaaa
    10861 aagaaaacct gctccatgaa atttttagta aaagcaggat aggcgatacc gaattgttcg
    10921 ccgtggacga gaatcttgta aaacgtttgt ttttatcgct tcgcggcgaa atcgtgttcc
    10981 cgaaaaacga aacggcggaa tcggaatttg aaaaaagcgt ccacgaacgc aggcaggaag
    11041 ggaatgccgg atccggtcgc aaacaactgc ttgatttggt acggcgcgga caccgggaat
    11101 acccttacgc gctgccccgg cttttggcgg gcgcggcatc ctacaagccg aaaaaatcga
    11161 aaatccgcct ttttaaagaa gcatatttac gcaaaagcgg tacgaggctg accgacgaaa
    11221 ttgcagacgg catccatatt tacacctgtt tttcgcgggc ggatttggaa aaagcctatt
    11281 ccgaatattt ggaacttttc aaatccgaat cggatgccga aggcagaaaa ccacggtaag
    11341 gtgcaaacag ataccgtaca cgttgaggag cagtatgatg ggcgattccg tcatttatta
    11401 tgtagaacag gcagacgaac cggtaaaccg tgccggcgaa cgcgcccgta aaacattcaa
    11461 atacttttgg cgcgagcttt tttgggaacg ccgccgcatc attcccgcct tagattttgc
    11521 catggtcaaa gccccttttt tccaagacgg cgaagacggc gaaatttgcg aacatatgtg
    11581 gatcgacgat atttattttg acggtcttta catttacggt gtgctgaaca ataaacccgg
    11641 cgaactgact aatgtcgaac aaggcgaaag catttgcgtt ccggttgacg acatcagcga
    11701 ctggatgttc gtgtgcaacg gcattcccta cggcggcttt accgtacagg caatgcgcgg
    11761 gcagatgacg gaagaggagc gcaccgaaca cgatgccgca tggggaatcg atttcggcga
    11821 tcccgggcag atattgctgg tgtatgaaga aaaaagaaca tcccgaaaat ctggaagagc
    11881 atccgatgtg ccggaactgt attgacgatt ttcggcaaca gttgtcccaa aactcggatt
    11941 atctgcggga acaggacgaa gacggctata cgccgcttca tcatgaagcc atcgcaggaa
    12001 atgcacttat ggttcaagcc atgcttgaat acggcgcaaa tcctgcctca acgacatcgg
    12061 aaggctatac cgccctcgat tttgcctgcc tgacgggctg gcaaaatgtt gccgacctgc
    12121 tcgaaccgcg acattaggca gacagttttc cgaaaacgaa cacaaacact ttttacagaa
    12181 agacaataaa aatgcccaaa atcaccgtac ttccacacac gacattatgc cccgagggtg
    12241 cagtcatcga taacgcaccc gaaggtaaaa ccgtccttga cgtgctgctc gaccatgata
    12301 tcgaagtcga tcacgcctgc gaaaaatcct gcgcctgcac aacctgccac gtgattatcc
    12361 gcaaaggttt cgacagcctg gaagagccga ccgaattgga agaagacctg ctcgatcagg
    12421 cttggggttt ggaagccgat tcgcgcctga gttgtcaggc ggttgtcgcc ggcgaagatt
    12481 tgattgtgga aatccccaaa tacaccatca accacgcgcg cgaagaacac tgaaaacagg
    12541 ccgtctgaag ccggcacgct tcagacggca ttgttgcgcg gataaggcgc aatcgcccga
    12601 aaacaggcgt tcgtacaggc ggaactttcg attctatagc gaattaacaa aaatcaggac
    12661 aaggcggcga gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ctgtaccggt
    12721 ttaaatttaa ttcactatat attgattttt atcggttttc tgacggaacg gattggtgcg
    12781 gcatccgagg cggattactc gggcgcgatg cacgggtatt tatcggtttt gcagccggaa
    12841 aaaccgccgg cgggttactt tcagggcggc ggtcgggcgg tatgccggat agcgttccac
    12901 tttcgcttct atattgattt tgattggttt tctgacggaa tgacccgatg cggcatccgg
    12961 gacggcttgt gttttttcct gtccgcctgc cggattttcc tgtccttgcg tgaaaccgaa
    13021 agagacggcg gcgcggcgga caagctcgag atagcgtcct tcaagctccg ggcaggcggc
    13081 ggacacgctt tctgccgtaa ccgtgaagcc gccgcccgac ccggaaaggc gttccgcaat
    13141 ctgcgtgtag atacgccaac gttgggcgcg caacattgcc gcatcgcaac cggcagccgc
    13201 tttatccaag gcaatcaggc cgcgccggtt ttggtggtga aggcaaaccg ttactctgga
    13261 gaggatatgt tgcccgtcca atatttgata cagtttgcgt atcagcagaa tcaggcggtc
    13321 aaactcctcc atctccgaca gggaatcagg atggtcggaa gcggtataaa ccagtttgga
    13381 caattttgcg gcaaacatat tgttcatcaa tcttccttgt cggttgaaac cccgctcttc
    13441 ggggcggtag aatcagattt gtttgggagg gacaactcct cccagatcag gacgacacat
    13501 aggctggtgc ttgatgtgtt gtccggcgag ttgaaacatt cagccatcct caaggtgcgg
    13561 cagttttgcc gaaacatatt ctacacggct tcaatgccgg acgataaaag gaaattcata
    13621 tgaaatggac cgacacccgg cgtatcgccg aagaactcta tgacctgcac ggcgaaacca
    13681 tcgatcccag aaccgtgcgc tttacccaac tgcgcgacct gattatggca ttgcccgaat
    13741 ttgacgacga ccccgcccgt tgcggcgaac gcatcctcga agccgtgcag caggcatgga
    13801 tagacgaggc ggaataagtt tcgggaatgc cgtctgaaat gcggcggtac gcggttcgtg
    13861 cttctgtttg cagcgggaat ggttttacca gtctcctttt ttcagcctgt ccagttggcg
    13921 gcggtcgcgc ttggtcggtc tgccgtcggg ataggcggaa gtgatgcggc tgaattggtc
    13981 gagctgtttg cgctcttccc tcaatgttgc cgttttcgcg tcctcttcat acagaagccg
    14041 cgcctcggat gccgggcggc gttggtggtt caaaccttta accttgattt tatagggaag
    14101 ggaattgagc gtcaggtcga taatatcgcc gatgtctatg gttttactgt ttttgacctt
    14161 cgagccgttt acttgaaccc tacccagttc gatgtgcttt tgcgcaaggg aacgggtctt
    14221 gaaaaaacgt gccgcccaaa gccatttgtc cagccgcatg gcggaagaat cgtgcttgtc
    14281 tttcatacga ttttgtttga aataattgaa tttgtttcaa gtttagcata agatacgccg
    14341 cctataacta gtatatatgc actaatccac tgttttccat gctgtccgaa cacaaaaaga
    14401 gggtgtggaa aagccgtttt ggacaataaa ttaactgcgg aatatgcaca aatagcgtat
    14461 gatagcggca gaatctgttg atgagagctt cattctatga aacctgtttt tttggatttt
    14521 gaacaaccca tagccgaact gaccaacaaa atcgatgagc tgcgtttcgt ccaagacgag
    14581 tctgccgtcg atatttcgga cgaaatacac cgtttgcaga aaaaaagcaa cgacctgacc
    14641 aaatcgattt tcagcaaact tacgcccgcc caggtttcac aggtttcccg gcatccgcag
    14701 cgtccctata ctttggatta cattgaggca ctgtttactg attttgaaga gctgcacggc
    14761 gaccgccact ttgccgacga tcatgcgatt gtcggcggat tggcgcgttt caacggacaa
    14821 agcgtgatgg tcgtcgggca tcagaaaggg cgcgacacca aagaaaaaat ccgccgcaac
    14881 ttcggtatgc cgcgccccga gggctaccgc aaagccctgc gcctgatgaa aacggcagaa
    14941 aaattcggct tgcccgtcat gacctttatc gatacaccgg gcgcgtatcc cggcatcggc
    15001 gcggaagaac gcgggcagtc ggaagccatc ggcaaaaacc tgtacgaact gacacgcctg
    15061 cgcgttcctg ttttgtgtac cgtcatcggc gaaggcggtt cgggcggtgc gttggcggtc
    15121 gccgtgggcg attacgtcaa tatgctgcaa tactcgacct attccgttat ttcccccgaa
    15181 ggctgcgctt cgattttgtg gaaaaccgcc gaaaaggcgg cggatgcggc tcaggctttg
    15241 ggcattactg ctgaccgcct gcaaaagctg gacttggtcg ataccgtcat caaagaacca
    15301 ttgggcggcg cgcatcggga ttttgggcaa accatgaaaa acgtgaaagc cgttttggaa
    15361 aaacaactgc acgaagcgca aagcatcccg cttgccgatt tgctttcgcg ccgtttcgac
    15421 cgcattatgg cttacggcaa attttcggaa caataattca ggtagaacaa gcagcaagca
    15481 gtttgtctga aactgcttgc tttttcttta tcgggacgga accgtgctga ctttagatgc
    15541 gtttgagcaa tgcttgaagg attgttttcc tcaaggtctg aatggaaaaa aaacagcggt
    15601 ggcattaagc ggcggcttgg attccgtcgt tttgctgcat ctgcttgtcc gcgccggaaa
    15661 aaagggcggt tttattccgg atgcattgca tatccatcac ggcttgagtc cccgtgccga
    15721 cgattgggca gatttctgcc aaaactattg cgatatgctc ggggtggggc tggaaacggt
    15781 taaggtctgc gtggaaaaaa acggtttggg catcgaggcg gcggcgcggc aaaagcgtta
    15841 tgccgcgttt gccgaaaaag gctttgacgt tttggcgttg gcgcaccaca gggacgatca
    15901 aatcgaaacc tttatgctgg cagtcgctcg cggcggcggt ttgcgcgctt tggcggctat
    15961 gcccaccgtc cgcccttttg gggaaaaagg catcatctgg cggcctttgc tgcctttttc
    16021 gcgccaagac atatgggatt atgcccaaaa acacggtttg ccgaatatcg aggacgaaag
    16081 caatagcgat acggcttatt tgcgaaaccg cttccggcac cgtattttgc ccgaactttc
    16141 ggcgcagatt ccccatttcg ggcggcatgt actgaacaat gtccgcgctt tgcaggaaga
    16201 tttggctttg ttggacgaaa tcatcggtca ggactgccgt tgggtctgtg aggaaggcag
    16261 tttcgatacg gtgcgttggc aggcgttttc cccacgccgg aaaacccata ttttgcggta
    16321 ttttttgaag gaaaacggca tccccatgcc gaatcagaat gcccttgccg acattgcccg
    16381 ggttttgacg gaggcaaaaa ccggacgttg gaacttgcaa ggctttgaat tgcatcatta
    16441 tgcaggcagg ctgtttgtgt tccgactgga aaaaacggat aaactgcggt ttttgaaaga
    16501 caggcagata agcggaaatt taagggaaat attgacgggg cagggatttg ttttaaaacg
    16561 tcatccgttt gggcttcctg agcatctttt ggagcaggac ggaattttga ggacggtagc
    16621 ggtatcggat acgttggctg tcggcggcat ccataaggat gtgaaaaaaa tccttcaggg
    16681 gaaacgggtt ttgcctgtcc tgcgcccaat ttggccgctt gttgccgaca gcggaaaccg
    16741 tccattggcg ttggcaaact gttgtgcgga tttccaatac tcggtttcag acggcatttt
    16801 gcccgtccat cctgactttc ccattttatt ttgataatat cgcaaacaga tttcggcggc
    16861 gttcagacgg gtattgtccg gttgcatatt tctaaaaggc ttgtgaagtg aaacacatca
    16921 gttcgaccaa taatgaacac atccggcacc tgcaccgcct gttgtcgcaa ggaaagttca
    16981 gacggcaata cgcccaaacc gttttggagg gcgtgcacct gcttcaggtt ttcctgcaat
    17041 ccggcagaaa accggtcggg gtatatattc ccgagggcaa aatgccgtct gaagaagtcc
    17101 gcaggctgag ggcggttgtg ccggaaggca aggttttttc cgtttcagac ggcatattga
    17161 aaaaaatcag cagcttgact tgtgcggatg atgtgcttgc gctgattgat attccggatg
    17221 cgggtgcttt gcctgccggc ggcgactgcg tggttttgga cggcgtgcaa gacccgggca
    17281 atgtcggcac ggtgttgcgg agcgcggcgg cggcaggggt aggtacggtc gttttgggca
    17341 ggggttcagc ggatgtttgg tcgcccaagg tattgcgcgc ggggatgggc gcgcatttct
    17401 tgttggacat ttattcgcag gcggatttgg aaatatggtt ggcgagctat aaaggccgtg
    17461 tgtttgccac cgccttgcga gaggaaaagc aggcggtttt gtacggcgaa gatttgtgcg
    17521 aaccgacagc ctgggtgttt ggcaacgaag gcgcgggggt cggtaaagcg gttttggaca
    17581 gggcggacaa gtgtgtcagg attccgatgc acgatgcaac cgagtcttta aatgtcgcga
    17641 tggcggcgac aatctgcctg tttgaacaga tgcggcagcg gtcggcgtat tgaggaaaag
    17701 aaatgccgtc tgaaaaaatc tattacggcg tattgatttt cttatgtatc gcttctatgc
    17761 tgctgtcgcc gtttttttat gcgggtgctt tgaagcccaa gaaggcggca ttgcggaagg
    17821 acgggcagtg gaaactcatc ttattgtcca ataccgtggc ggcggcggtt ttgttttgga
    17881 tgtggtggaa atggttttga cagatattgc tcaaaattgg gttaatggaa tccgaacaaa
    17941 taaataattt ggtaaaaaat ttgttaaatc aatggattaa agtttttcgg gaaacaaaac
    18001 agctctaagc aaatagggcg tttgtcggta aatacggaag agttgcggca ttatcgggca
    18061 tctttaacaa gtagtgccgg cttggcaggc aattggtttt tatgggcagc ttgcaaaatc
    18121 gcggatataa aattgcgaat cggttaaagt gtggggacgc tatgaaaaat tgcgaatttt
    18181 tttatgaccc gacaagggca atctatgata gcggtgcaga ttatttaacc agagaaaaac
    18241 atagattagt cgtaattgca aatagtgctt gggggctatt gcttaattta tcttgttatt
    18301 atgacgaggt tttggaaaag cggaaaatac cgttcggcaa acaggaaatt gatgacgata
    18361 tggacaaagt gtccgccctt aagcggaagt ttaaagatat ttctgaaatc aaagtagggg
    18421 atggttggga atacccgttc aattatgagc agggaatgaa agaattagat gaagtgctat
    18481 tgaaatacat tccctttttt gaagaagaac gataaaggag gttgatatgc gcgtatctaa
    18541 aataattgga agtatgttgc ttgttacagc ggttcagacc gtattttcgg caaatgttta
    18601 cgcgtgccgc cataatggta aaaccagtta cagccaaact ccgggaaaac attgtaccaa
    18661 cgcgggtttg gggcgggacc tggtgtacag ttcggttaga ccggcagtaa aagacagggc
    18721 ggaagacgca ggggtcggcg attattcgga cacggtgagg gacgaacacg tccaaaatcc
    18781 gagagagaat acgcataagg atgcttcgaa taccggtgca aagacgcatt gattgaaacc
    18841 gaatcagcca tgacggcgtt gaataataga atttgaacga ttagggaact ctgatgaaac
    18901 acatccacat tatcggtatc ggcggcacgt ttatgggtgg gattgccgcc attgccaaag
    18961 aagcagggtt tgaagtcagc ggttgcgatg cgaagatgta tccgccgatg agcacccagc
    19021 tcgaagcctt gggcataggc gtgtatgaag gcttcgacac cgcgcagttg gacgaattta
    19081 aagccgacgt ttacgttatc ggcaatgtcg ccaagcgcgg gatggatgtg gttgaagcga
    19141 ttttgaaccg tgggctgcct tatatttccg gcccgcaatg gctggctgaa aacgtgctgc
    19201 accatcattg ggtactcggc gtggcgggga cgcacggcaa aacgaccacc gcgtctatgc
    19261 tcgcgtgggt tttggaatat gccggactcg caccgggctt ccttatcggc ggcgtaccgg
    19321 aaaacttcag cgtttccgcc cgcctgccgc aaacgccgcg ccaagacccg aacagccaat
    19381 cgccgttttt cgtcattgaa gccgacgaat acgacaccgc gtttttcgac aaacgctcca
    19441 aattcgtgca ttaccgtccg cgtaccgccg tgttgaacaa tctggaattc gaccacgccg
    19501 acatcttcgc cgatttgggc gcgatacaga cccagttcca ccacctcgtg cgtaccgtgc
    19561 cgtctgaagg cctcatcgtc tgcaacggac ggcagcaaag cctgcaagac actttggaca
    19621 aaggctgctg gacgccggtg gaaaaattcg gcacggaaca cggctggcag gccggcgaag
    19681 ccaatgccga tggctcgttc gacgtgttgc ttgacggcaa aaaagccgga cacgtcgctt
    19741 ggagtttgat gggcggacac aaccgcatga acgcgctcgc cgtcatcgcc gccgcgcgtc
    19801 atgccggagt cgacattcag acggcctgcg aagccttgag cacgtttaaa aacgtcaaac
    19861 gccgcatgga aatcaaaggc acggcaaacg gtatcaccgt ttacgacgac ttcgcccacc
    19921 atccgaccgc tatcgaaacc acgattcaag gtttgcgcca gcgcgtcggc ggcgcgcgca
    19981 tcctcgccgt cctcgaaccg cgttccaata cgatgaagct gggtacgatg aaagccgccc
    20041 tgcccgcaag cctcaaagaa gccgaccaag tgttctgcta cgccggcggc gcggactggg
    20101 acgttgccga agccctcgcg cctttgggcg gcaggctgca cgtcggcaaa gacttcgatg
    20161 ccttcgttgc cgaaatcgtg aaaaacgccg aagcaggcga ccatattttg gtgatgagca
    20221 acggcggttt cggcggaata cacaccaaac tgctggacgc tttgagatag cccgggcgat
    20281 gccgtctgaa agcccttcag acggcatcgc ccggctgcgc ggcacaaagg cggaaaaacc
    20341 gtttgccccg tattttcaaa cgcgttacac ttgccgccgc tgttttcagc catttgatta
    20401 cccgcaaccg ccgtcattgc gccggcggtc tgcacgtcag cgtcattgcg ccgctgtaaa
    20461 tacgaaagaa cacattatga ccgtatcccc cgtagccttg cgccgcaaga ccgagtgcaa
    20521 gccgcatccc accgcgcgct attggaaaaa atgcgatgtc gaagccctgt tcggacttcc
    20581 cttcctcgac ctcatttacc aagccgccga aatccaccgc caaaatttca acccgcgcga
    20641 aatccagctt tccacgctgt tgtccatcaa aaccggcggt tgtcccgaag attgcgccta
    20701 ctgtccgcaa tcggcgcacc acaacaccaa tctgggcaaa gagcagatga tggatgtgga
    20761 tgaaatcgtc gaaaaagcca aaatcgccaa atcgcgcggc gcaagccggt tttgtatggg
    20821 cgcggcatgg cgcggcccca aacccaaaga cgtggagacg gtttccgcaa tcatcaaagc
    20881 cgtcaagggc ttgggtatgg aaacctgcgg cacgttcggt atgctcgaag aaggtatggc
    20941 ggaagacttg aaagaggcgg gcttggatta ttacaaccac aacctcgaca ccgaccccga
    21001 ccgctacaac gacatcatcc atacacgccg gcatgaagac cgtatggaca ccttgggcaa
    21061 agtccgcaac gccggtttga aagtctgctg cggcggcatc gtcgggatga acgaaactcg
    21121 tgccgaacgc gccgggctga ttgccagcct cgccaacctc gacccgcagc ccgaaagcgt
    21181 gccgattaac cggttggtca aagtggaagg cacgccgctt gacgatgccg aagatttgga
    21241 ctggacggaa tttgttcgca ccatcgccgt ggcgcggatt acgatgccgc aaagttatgt
    21301 ccggctgtcg gcagggcgca gcaatatgcc ggaagcgatg caggcgatgt gctttatggc
    21361 gggcgcgaat tcgatttttt acggcgacaa gctgttgacc acaggcaatc cggacgaaga
    21421 cggcgacaga atcctgatgg aaaagcttaa cctgtatccc ttacagtttg agctggaagg
    21481 cgaggtcgcc gaagtggaaa aagcctcggg gattaaagtg gattattgac gattgcaaaa
    21541 tgccgtctga aacccggaaa aaggctttca gacggcattt gttcggacgg tatttccaat
    21601 atctttttac cggcgcgtga tgttgccgtc gggcgagaca tccagcccgt tcccgttggg
    21661 gaaatcgctg cggttcagat aaataatccg cccgataacg gttttctcgg ggtcgatttt
    21721 ggggatttta tcgtcggctt gggtaatagg gatgatagtt ccggaaataa atttaccctg
    21781 tttgtcggtg ataattttga aaatcggcgc aatgccgctg atgccggagg tgttgattgc
    21841 gccgtaggtg gcaaagttgc cgccgctgta ggagatgaag cggtcgcggt aaagttcgac
    21901 ggcgcgtgta acgtgcggcc cctgcccgaa tacgacatcc gcgccggaat cgacggcaag
    21961 ccgcgcaaac tcaacgacgt tgcccctgtt ttccccatag aagatttcgg tatcgaacgg
    22021 caggtgttcc gcctgtttcc cttctgcgcc gccgtggaac atcacaataa cgatctcggc
    22081 tttttgtttg gttttggcaa tcagtttttt gactttggca tagtcgttca gtttgacggc
    22141 ggcaaggttg ggggcgaagg agacgaagcc ggatcttacg ccgtttttct tcaggatggc
    22201 ggtttcaaac ctgttttcga tgcccgaata tttgatgttc aattcgtcaa ggttcgccct
    22261 ttatgccgtt tccgcaccgc aaaccgccgg ggtcaagccc tcggcgcatc ctgccggaac
    22321 ggaatccccg tgctgccgat tgacggttca aaccccgcgc ccgtttcaaa taccggcgat
    22381 gtggacggac aggatgcgcc tgacgaaaag acagccgata ccgtttccat tatcggcgtg
    22441 ggcgacatta tgctcggcag caattatccg gtcgattacc tgcccgatac caatattctg
    22501 aaaaacgtcg aatctgcctt gcaagacgcg gacattaccg tcggcaacct cgaaggcacg
    22561 ctgtttgacg aaggcggtac gccgaaaaaa tgtgcaaccc cccaaaatat gctatgcatt
    22621 ccgaacgccc tccgcatacg ggcaatacct tgccgacgcg ggattcgact acctcagctt
    22681 cgccaacaac cacagcaacg acttcggcgc gcaaggcatc acggcaacgg cggcgagcgg
    22741 cagctctttt acatactcga tcgcgctaaa gccgctgccg ataacgaggc caaaattgcg
    22801 gaaaataccg ccatcgccca gataaatttg tccatcatca gacctttact gttcagacga
    22861 gacagcattt gccgcacgtt ttggggctta tctttcgatt tgcgctacgt cgcgcaccgc
    22921 gcctttgtcg gcggaagtcg ccatcgcgcc gtaagctctt aatgcggcgg agacgtagcg
    22981 gtcgcggttt tccggtttcc acgctttgct gccgcgtgct tccatttcgg cacggcgggc
    23041 ggcgagttct tcgtcggaaa tgacaaggcg gatgctgcgg ttggggatgt cgatttcgac
    23101 ggtatcgcct tcgtgtacca aaccgatcgc gccaccttcc gccgcttcgg gcgaggcgtg
    23161 tccgatggac aaacctgatg tgccgccgga gaagcgtccg tcggttaaga gagcgcaggc
    23221 tttgccgagg cctttagatt tcaggtagga agtcggatac agcatttcct gcatgcccgg
    23281 gccgcctttc gggccttcgt agcggatgat gacgatgtcg ccggcgacga tttggttgcc
    23341 caaaatgcct tctactgcgt cttcttggct ttcaaacacg cgggcgcggc cggtgaattt
    23401 gaggatgctc tcgtccacgc ctgcggtttt taccacgcag ccgcgctcgg cgatgttgcc
    23461 gaacaagacc gccaaaccgc cgtcttgcga gtaggcgtgt gccacgtcgc ggatacagcc
    23521 tttttcgcgg tcgaggtcga gggttttcca catacggttt tgcgagaacg cttgggtggt
    23581 gcgtacgccg ccgggcgcgg ctttgaagcg ttcgatggcg tgggtgtttt cagggttggt
    23641 tacgtcccat ttttcaatcg cgtctttcag cgtcggcgcg tggatagtgt gcacgtcggt
    23701 gtgcagcttg cccgctttgt ccaattcttt caggatggcg aagatgccgc cggctcggtg
    23761 cacgtcttcc atatagtagt cgtggttgtt gggtgcggtt ttgcagatgc agggcacaac
    23821 gcggcttaag cggtcgatgt ctgccatttt gaaatccacg cccgcttcgt tggcaacggc
    23881 gagtaaatgc aaaatagtgt tggtgctgcc gcccatggca atgtccatgg tcatagcgtt
    23941 ttcaaacgct tttttggtgg caatgctgcg cggtaacacg gtttcatcgt tttgctcgta
    24001 atagcgtttg gtgatttcga caatcatacg gccggcttcg aggaacagtt ctttgcggcc
    24061 gacgtgggtg gcgaggtaag aaccgttgcc cggcagggac agcccgagtg cttcggtcag
    24121 gcagttcatc gagtttgccg taaacatacc cgaacacgag ccgcaggtcg ggcaggcgtt
    24181 ttgttcgact tcttcaactt gccggttgct gatattgtcg tccgccgatt caatcatcgc
    24241 gtcaatcaag tccaaacggc gttcgggctg gatgtttgcc acgccgataa ctttacctgc
    24301 ttccatcgga ccgccggaaa cgaagatggt cgggatgttc aggcgcatcg cggcaatcag
    24361 catgcccggg gtgattttgt cgcagttgga aatgcacacc agcgcgtcgg cgcagtgggc
    24421 attgaccata tattcgatgg agtcggcaat caaatcgcgg ctgggcaggg aatacagcat
    24481 accgctgtgt cccatagcga tgccgtcgtc gatggcgatg gtgttgaatt ctttggcgat
    24541 ggcgccggct ttttcgattt cgcgggcaac cagctggccc atattgtgca ggtggacatg
    24601 acctggcacg aattgggtga acgagttggc aacggcgata atcggtttgc cgaagtcggt
    24661 ttccatcacg ccggtggcgc gccacaacgc gcgcgcgccc gccatattgc gaccgtgggt
    24721 ggaggttttg gagcggtatt ctggcatagt gtgtttcctt gtgcctatac cgtctgaaag
    24781 acagggctgt ttcagacggt atcgggtacg gttttttaga gtgggaaaag agggtatttt
    24841 ataccaagta tcggaatttt gcgggattga aacggcgtgc ggcaaaaaag aaaatccccg
    24901 caggaatgcg gggacgggtt caggcgcggg caatcgcgac ggctttggat gcgtcccaaa
    24961 aatcaacggg tgcatttaat acgggtttga cgatgcccgt ccgtatggcg aaatcgccga
    25021 aaccttcgcc gatattgcgt tctgccgccc atttgccgat caggtcgtcc aattcggcaa
    25081 ggatttcggg cagggtgatg ttttctttgt aaagacgggg gatgcgtacg ccttcacggt
    25141 cgccgccgat atggaggttg tagcgtccga cggctttgcc gaccagtccg atttccgcca
    25201 acatcgcccg tccgcagccg ttcgggcagc cggtaatgcg ggtaacgatg tagtcgtccg
    25261 acgtgccgtg tttcgccata atcttatcca gctcgccgat gaagtccggc agcacgcgtt
    25321 cggcttccgc cattgccagc gggcaggtcg ggaaggaaac gcacgacatc gcattttcgc
    25381 gcagtttgct gacaccgtcg tgtatcagcc cgtatgagcg ggcgagctgt tcgatccggg
    25441 ctttgcctcc ttcgggtaca tttgccacga tgaggttttg gttggcggtg atgcggaagt
    25501 cgcctttgtg gattttggcg atttccaaca cgccggtcag aagctgtttc ccgccttcgt
    25561 caaccaaacg cccgctttcg atgaaaaggg tcaaatgcca gttgccgtct atgcctttca
    25621 cccagccgat gcggtcgccg cgcccggtaa atttgaacgg gcgtatgggt tcgaacggca
    25681 tacccatacg gcgttcgact tccgcgcgga agttgtccaa gcccatattt tgaatggtgt
    25741 agcgggtgcg ggcgtttttg cggtcgctgc ggttgccgaa gtcgcgctgt gtggttacca
    25801 ccgcttcggc ggccttcagc gcgtgttccg gaggcacgaa acccagttcc agtgaaatgt
    25861 tcggataggt tttggtgttg ccgtgttcca tcgaaagccc gccgcccgcc aaaacattga
    25921 atccggcaag ctgtccgttg ccgtctgaaa tggcgacaaa atccaagtcg ttgccgtagc
    25981 agtccacatc atttaagggc gggatgacga ctgcggtttt gaatttgcgc ggcagatagg
    26041 ttttgcccaa aatcggctcg tcttcttgaa ggaagtcgtc agaactttga acttttttgc
    26101 cgtccaccca cacatccaga taaccgcgcg tgcgcggcag caagtgttcg gaaatctttt
    26161 tcgcgtattc gtaagcctgc cggtgcagtt cggactcgat cgggttggac gtgcaaagca
    26221 cgttgcggtt catatccgcc gccgtggcga tggaatccaa acccagtttg tgcaagaggc
    26281 ggtgcatcgt ctgcaacttg gctttcggca cgccgtgaaa ttggaaggtt tgccggttgg
    26341 tcagccggat ggagcggtaa tgactgtttt cccgggcaaa tttgtccagt tctatccatt
    26401 gggacggttt gatgatcccg cccggcagcc ggcagcgcaa aagcataaat ttcaagggct
    26461 cgagttttgc ctcggcgcgt tcggcgcgga tgtcgcggtc gtcctgctca tacataccgt
    26521 ggaagcggat gagttggaag ttgtcgcctt tgaagccgcc cgtgagcggg tctttcaaat
    26581 cgtccaaaat cgtgccgcgt aaaaaattgc tttcggtttt cagacgttcg ttgtcggata
    26641 gcggtttttc ttgccacgcc aaaccttttg tcttggtctg tacggtcatt ttgtgttcct
    26701 cccgattata tttaatcaat aaacatcacg ctgatagcgt ttttcttcgc gcagcatatc
    26761 caaatattct tctgcgccct cttcgtccaa atgtcctgcc ccgataatca catccagcaa
    26821 ggcggcttcc acgtcttttg ccatttttgc cgcatcgccg cacacataga tatgcgcgcc
    26881 ttcctgcagc cattgccaaa gtccttccgc ctgttcgcgg attttgtcct gcacatagat
    26941 tttttcttcc tgatcgcgcg accaggcgaa atcgtatctg tgcaggaagc cgtctttggc
    27001 aaactgctgc cattcggttt gatagagaaa atcacgggca aaatgcggat tgccgaaaaa
    27061 cagccagttt ttgccttccg cattttctgc ggcacgttgt tggacgaaag cgcggaacgg
    27121 tgcgacgccg gtgcccgagc cgatcatcac aatcggcttg cggctgtctt cgggcagcct
    27181 gaagccgtcg ttgcgttcca caaacacgcg caccgtgccg tcctcttcca gccggtcggc
    27241 aaggaaaccc gatgcgccgc ccgccctggc gcgcccttcg tgttcgaaac gcaccgcgcc
    27301 gacggtcagg tgcacttcgt cccccacttc cgcctgcgac gaggaaatcg aatacaggcg
    27361 cggcgcaagc gggcgcagta ggccggcgaa ttgttccgct gtcagttttg ccgggaagcg
    27421 gtgcagcaca tcggcaatcg gcgtgctttg cacaaagcct tgcaaaacgg cgttgtcggc
    27481 agcaatacgg tcgagttcgt catcatcggc gaacggggca tagcctttga caaaggcggg
    27541 ggtgttttgc gtgagttcaa aatgggataa cagtgcggag gcaaccggca gggtttttcc
    27601 gcccgcctgt atttccgttg cctgatcgat gccgagcagg tctaggattt ccctgaccag
    27661 tgccggatcg ttgtcaaacc aaacgccgag cgcgtcgccc gggaggtagt gcaaatccga
    27721 accgctcaaa tcgatttcga tgtggcgcac gtctttatcg gattggcggg cggtgatttt
    27781 ctgattggcc agcagggcgg cgggaaaggg gtctgccttg cagtacctgc catccggtgc
    27841 cgtctgaagg ccggcggggg gcgttgtctg cggcgcgggc gttgcccggt tttttgcggc
    27901 ttcttctttt aagagtgcgg cgatattatc tgtccatccg tctgcggcgg cggcaaagtc
    27961 caaatccgca tcaacgcgtt cgagcaggcg ttttgcgccc aattcttcaa aacgtttgtc
    28021 gaaatctttg cccgcccggc agaaattcgg ataggagctg tcgcccaaac ccagtacggc
    28081 aaattggagt ttgtccaatt tcggggcttt tttgccgttc agcagtttgt gcagcacgac
    28141 ggcttcttcc ggcggttcgc cttcgccttg ggtggaggta accagcagca ggcggcgttc
    28201 gccggcgatg tttttcgcct tatagtcttt cagttcggcg cgactgactt ggatgccggc
    28261 ggcttccagg ctgtccgccg ctttgtcggc aacggatttc gcattgccgg tttgcgaggc
    28321 ggaaaggacg gttacggaaa aaggttctgc cgtcggcaat gccgtctgaa gcgcgggcag
    28381 tcctgcagat gccccgtttc ctgcttttgc ccaagcgtag ccggacagcc acgcccattg
    28441 tgccgcgtcc agccccgaca ggagctgcgt gatttcgggc ggcataggcg gtaatggcgg
    28501 atttgtgttc tgcatatcgt gttcactcat aaaatcatac ctgccgcaac agtgccgtat
    28561 gtcgcttcgt ctatcaggat aaacgaaccg gcggcggtgt tttccgcata aggcgttgcc
    28621 gtaacgggtt tttgaaggtt gatgcggact ttggcgatgt cgttcatctt caaggattcc
    28681 gcgccggcct cttgttccag cgtgcggaca tctaaaacgc tttcaatttc cccgactttt
    28741 gccggcacgg tttgcgtgcc gtgcttgagc aggtatttgc gcgcggtgtt gagcggacgt
    28801 tcgtcaaacc agcaaagcgt ggcttccaga tgtttttgcg gggcgagcgg ggaattttta
    28861 tcgacaaaaa ggtcgccgcg cgaaacatcg atgtcgcgat ccagccggag ggttgccgcc
    28921 tctccggcaa aagcctgcgc cacttcgcct ttgggtgtga tgatttcaga cacttcggcg
    28981 gtcagcccat tcggttcgat gcggacggtt tgtccgacgg cgaccgaacc gcgttcgatg
    29041 cgcccctgat agcctcggaa atcatcggcc ttgtcggcat cttggcggac gaccagttgc
    29101 acggggaaat aaaaatcgtc ggcggtgcgg ctgacttcgt ccgcccccgg cagggtttcc
    29161 aaaatggaca ataagggttc gcctttatac caaggcatat tgccgccggg gtaaacaatg
    29221 ttgtcgccca agagtgcgga caccggtacg aaatgcgcgt ctttcaaacc gagctgttcg
    29281 gcaagtcggc ggtatgcctc cacaatggcg ttgaatttgt cttcgctgta atccagcagg
    29341 tccattttgt tgaccgccac cacaatatgc gggcagtcga gttggcggag gatggcggaa
    29401 tggcgtttgg tctgcggcag aagctgcaag ggctgcgcgc cgaaatccag ttgggatgcg
    29461 tcaaccagca cgactgccgc cgaagcggtg cttgcgcccg taaccatatt gcgcgtgtat
    29521 tgttcgtgcc ccggcgtgtc ggcgatgatg aattttcgtt tcgccgtgga aaaatagcgg
    29581 tatgccacat cgatcgtaat gccctgttcg cgttcggctt ccagtccgtc ggtcaggatg
    29641 gagaagtcta tggtttcttt caaacctttg cttttgccgg attccaaggt tttgatttgg
    29701 tcggacagca gggctttgct gtcgtagagc agtcgtccga ttagggtgct tttgccgtca
    29761 tcgacgctgc cggcggtaat gaagcggagc ggggtttggt gttgtgccgt catattttct
    29821 tcctcatatc tgcttaaagg gtttttgaaa tttagaaata gccttctttt ttgcgttttt
    29881 ccattgccgc ctcgcttgcc tgatcgtcca gccgggtcgc gctgcgttcg gaaatgtcgg
    29941 caaccgctgt ttctctgata atctccgtcg gcgtggacgc ggtgctttct accgggcagg
    30001 tgcagctgat gtcgccgacg gtgcggaagc ggacatcaag gatttcggag gtttcagacg
    30061 gcattttcgg ggtgagcggc gttacgggga ccagcagccc cctgcgtctg accacttcgc
    30121 gcctgtggct gtaataaatc ggcggcagct cgaggttttc gcgggcgatg tattgccaga
    30181 tgtcgagttc cgtccagttg gaaatcggga agacgcgcat attttcgcct ttatgcagcc
    30241 tggtgttgta cagcgaccac agctcggggc gttgcgcctt cggatcccat tgtccgaact
    30301 cgtcgcggaa cgagaaaatc cgttctttgg cgcgggcttt ttcttcgtcg cgccgcgcgc
    30361 cgcccataag cgcgtcgaag ccgtttgcct cgatggtttc caacaaggta accgcctgtg
    30421 ccgcattgcg cgaatcggtt tctttgcgta agaccaccgt gcctttggca atggagtctt
    30481 ccacgcgccc cactatcagg cgggcattga gttttgccgc ctgcgcgtcg cggaaagcaa
    30541 tcacttcggg gtagttgtgt cccgtgtcga tatgcaccag cgggaagggc agtttcaccg
    30601 gccggctgcc cagccggaag gctttgcagg cgagggcgag caggaccacg gaatctttgc
    30661 cgccggaaaa gagcagggcg gggttttcgc attctgccgc cacttcgcgg ataatgtgga
    30721 tggattcgga ttccaaccag tcgagttgga cgttgttcgg ttcggttttc gtcataccat
    30781 attccttatt tcttctgttt gatccttatg aattatttgt gcagcccgca ttctttgctg
    30841 tttttatctt cccaccacca ccgcccggcg cggatgtctt cgccggcctt gacggggcgg
    30901 gtgcaggggt cgcagcctat actgggaaag ccttgccggt acaaatcgtt gtaaggcaca
    30961 ttgttggcaa ggatgtatgc ccacacgtcg tgttccgacc agtcgaaaat cgggttgtat
    31021 ttatcgatgc cgcgcccggc atcatattcg gcaaacggca gttccgtgcg tgtggcggac
    31081 tgttcgcggc gttgcccggt aagccaagca tccgccccgg caatggcgcg gtcgagcggt
    31141 tcggtttttc ggatgcggca gcattcgcgg cgcgcttcaa cgctgtcgta aaaggcaaac
    31201 ctgcctttgc tttccacata atgcagggca tcttcccgta tcggttggaa acgcttgatt
    31261 tgcatatgcg gataaacacg ttcaatccta tccaacaggt ttaaagtttc cgcgtgcagc
    31321 agccccgtat ccaaagtaaa aataccgata ttgaggtttt ctccggcgat aaggtcggta
    31381 attaccatat cttccgcagc aaggctgctg gcaaaccgtg catcccggtg gctgccggca
    31441 atccggtgca ggcgttgttt gagggtttcc gttttttccg ccaaagccgt ttcactgcca
    31501 ttttcaatcg gcggaatttt ccataaagca ggtttgaaaa ttttcatttt gtccgccttg
    31561 ctttatgcgg cttgttgttc ttgtccggtg cccagtccgt ccaatgcaaa atacgcgtgt
    31621 tcggccggat tgctttcttt tttggcgttt tcgccgaacc aagccagttt ttcgtgcagc
    31681 cccaccactt cgcctatgac aatcaatgcc ggattcggcg cggtttcggc gagttcggca
    31741 aggttggcga gcgtgccggt tgcggttttt tgagccggca gcgtgccttg gctgatgacg
    31801 gctgccggcg tgtcgggcga gcgtccgtgc tgttgcagcc gttcggcaat cagggcggct
    31861 ttgagcgcac ccatataaat caccaaggtc tggcggctgc gggcaagggt ctgccattcg
    31921 atgtcgggcg catccgcctt gcggtggccg gttacgaaaa ccgcactttg ggcataatcg
    31981 cggtgcgtga gcgggatgcc ggcataggcg gtcgcgccga cggcggcggt aatgccgggg
    32041 acgaccgaaa acggaatctg atggcgtgcc aaggtttcca attcttcgcc gccgcgtccg
    32101 aacacgaaag ggtctccgcc tttcaaacgc accacgcgcc tgccttcgcg ggccagcctg
    32161 accataagcg cattggtgtc ctcttgcggg gtgcggccgc cccgggcgcg cttgccgaca
    32221 aaaatccgtt ccgcatcgcg gcggacgagg gacagtatgc cgtctgaaac cagcgcgtcg
    32281 taaagcacca cgtctgcctg ctggatttcc tgcagccctt tgagcgtcag cagccccgca
    32341 tcgccgggac ccgcgccgac cagcgagacg gagccgcctt gatcattttg acgactttgt
    32401 tccaattggc ctgccaattc ccgttcggca agggtgtttt gccggttttt gacgagggcg
    32461 gcgaaacgtc cgttaaactg cttttcccaa aagcggcggc gttcggtaac ggatttcagt
    32521 ttgcccttga cggcatcgcg ccaccttcct gaaatttccg ccatatcgcc caaagacggc
    32581 ggcagcaggg cttccagcct ttcacgcagc agtcgggcga ggacgggcgc gctgccggag
    32641 ctggaaacgg caatctgaac cgggttgcgg tcgataaccg atgggaagat gaagctgcaa
    32701 tggtcgcggt catccaccac gttgaccggc ttttggcagc tttcggcaag atggaaaacg
    32761 cgccggttga gggcttggtc gctgcttgcc gcaatgatga ggaaaaccgt gcggatgtgt
    32821 tcggcacgaa attcttcggc aagccacagg attttgtttt ccgccgccaa cgcggagagt
    32881 tcggcattca ggtgttttgc ggcaaccctg acctctgcgc ccgccttcag cagcaggctg
    32941 attttgcgtg cggcgactgc gccgccgcct acgaccaata cggggcggcc ggcgaggttg
    33001 gcgaaaatgg ggaaataatt cactggctga ctcctttgct gtttgcccgc accttgttgc
    33061 cgatacggtg cgtcgcggca tttttgtcgg aatgcgggtc attttagaca aaaggatttt
    33121 cccggttaaa taataaaaag gcatttgtta gaagctgaaa gctatatggg ggcggctgcg
    33181 gatgcggcgg ttttccgttt tataacggtt tgggacggaa aaacggcctg aagccgtttc
    33241 gggcattcag accgtttgcg cggaggggga atgccgtccg aagggcgaaa agggcttcag
    33301 acggcattga tgtcgggttt caggacagga gcaggatggc ggctgcggca agcgcggcaa
    33361 ccgataatgc ggcggcaagc gcggctttgc cggcaaagcg gattgaggtt ttgccttcga
    33421 tgtatttgaa gccggttatc atcgggagga cgaggttttt ctttttgaac acgcggtatg
    33481 cggcgacggc ggcgatgtgg actgcggaaa aaacggcgag cagtttgaaa aagttgagat
    33541 ggattttccg cataaggctg cccgtatgtt cggaaaccaa atggttgagg tagccgttgg
    33601 tgctgaaggt gttttcatcg gcggcaaaaa gccccgtgcc gacttggaac gacacggcgg
    33661 ccaaaagcgc aacgaccatc agtgcgccca aggggttgtg tccgggttgg acgtgttcgg
    33721 gaataccgtt tttcatatac tctctgatac ccgaccatcc gcggacgaaa cgggagaaac
    33781 gggcggtatc gctgccccaa atgccccagc agaggcggaa tacgagcagg aaaaggataa
    33841 acagcccgac gcgcgtgtgc cattgcagca tatcgccgcc ggttttcgcg ctataccaca
    33901 taaagggtag ggatgcggca agcagccagt ggaaaaggcg ggtggggaag tcccagactt
    33961 tggttttgtt tttcataatc ggtttccggc ggtagaatcg gtttgttttc gagccttaat
    34021 tttaaccgat tggaggggaa atgtttcccg ttttccatct ttcaggcgaa agccgccgcc
    34081 ggatgcttca ggcggcattg cgttttcctc atgttttcaa agcccgtgcg gaagattcgc
    34141 acaaagggac tttcggcacg ctcgccgtag tcggcggcgc ggtcggcatg agcggcgcgc
    34201 ccgtattggc ggcatcggcg gcaatgtatc tcggctgcgg caaagtgtgg gcgggcttca
    34261 atcaggaaac gcttccgttt gccgtcattg ccggttttcc cgaaattatg ttggacacgg
    34321 cagacggttt gaccaagcgt caaggcataa acgcttggac ggcgggttgc ggcttgggta
    34381 cggataaggc ggcggtcgga acgcttgccg gaattttgac ggaacacacg gacaagcccg
    34441 tccttttgga tgcggacgcg ctgaacatat tatcaaccga tgccgaaacc cgaaatctgg
    34501 cacgcgggtg taaaaacctg attttaacgc cacaccccgc cgaagccgcg cgcctgcttg
    34561 gaacgacggt tgcacaggtt caggcggatc ggacggcggc agtgaggaag ataggggcaa
    34621 ttttcggcgc aaccgtggtt ttaaaggggc acaaaacatt ggttgccgcg tccgatacgg
    34681 aaatctatgt caacgaaagc ggcaacgcgg gattggcaac ggcgggcagt ggcgacgtat
    34741 tgggcggcat catcggcagt ctgctcgcac agggcgtgcc ggtttttgaa gccgcctgcg
    34801 cgggcgcgtg gctgcacggc gcggcggcgg atgtcataaa agaatcggca ggcattgcgg
    34861 cagggctgtt ggcaggggaa atcgccccgg cggcaaggtg gctgcgtaac cggataactg
    34921 aaagtatgta agaa
//
LOCUS       DAWKQB010000004        33689 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.004, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000004 DAWKQB010000000
VERSION     DAWKQB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.004"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..1034)
                     /locus_tag="WJL14_000121"
     CDS             complement(120..1034)
                     /locus_tag="WJL14_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7655253.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WJL14_000122"
     CDS             1290..2759
                     /locus_tag="WJL14_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ7655254.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVAAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAATVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FTGRVLIPVVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WJL14_000123"
     CDS             3011..3382
                     /locus_tag="WJL14_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655255.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVE
                     SQCRAELNKRSEWRLTALAMSAEKQAEWENKICACVAQEAPNQLTGNDVMQMLDPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WJL14_000124"
     CDS             3455..4864
                     /locus_tag="WJL14_000124"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ7655256.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLSYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPNGKMSAFQRAQMYSLQDENIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4911..5705
                     /locus_tag="WJL14_000125"
     CDS             4911..5705
                     /locus_tag="WJL14_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655257.1"
                     /translation="MTVPMQETHFSILLDELAAKQEAAIAPHLLADGTKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAAGIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5893..6669
                     /locus_tag="WJL14_000126"
     CDS             5893..6669
                     /locus_tag="WJL14_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7655258.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7429..9900)
                     /gene="cadA"
                     /locus_tag="WJL14_000127"
     CDS             complement(7429..9900)
                     /gene="cadA"
                     /locus_tag="WJL14_000127"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEZ7655259.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGSDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQTLETLAQTTD
                     IIFDKTGTLTQGNPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILKHRVSDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVWQLAGKNLTLHILSGDRETAVAETARALGITHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRQIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQ"
     gene            complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="WJL14_000128"
     CDS             complement(9897..11075)
                     /gene="hflX"
                     /locus_tag="WJL14_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ7655260.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11080..12360)
                     /locus_tag="WJL14_000129"
     CDS             complement(11080..12360)
                     /locus_tag="WJL14_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ7655261.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVGGMAGSSITLAPEDSPERGVTLYLQD"
     gene            complement(12513..13232)
                     /locus_tag="WJL14_000130"
     CDS             complement(12513..13232)
                     /locus_tag="WJL14_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ7655262.1"
                     /translation="MDTTRIVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAMLAS
                     GIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAKGM
                     EFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAAAP
                     YDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPIKL
                     GVLYYGDESTQYEPAPIRVDK"
     gene            complement(13303..13980)
                     /gene="radC"
                     /locus_tag="WJL14_000131"
     CDS             complement(13303..13980)
                     /gene="radC"
                     /locus_tag="WJL14_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ7655263.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14103..15452)
                     /gene="gshA"
                     /locus_tag="WJL14_000132"
     CDS             complement(14103..15452)
                     /gene="gshA"
                     /locus_tag="WJL14_000132"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ7655264.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15712..17121
                     /gene="leuC"
                     /locus_tag="WJL14_000133"
     CDS             15712..17121
                     /gene="leuC"
                     /locus_tag="WJL14_000133"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ7655265.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVEGRLKPGVTAKDVALYIIGQIGTAGGTSYAIEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKGKPFAPKGEAWDKAVEYWRTLVSDEGAVFD
                     KEYHFKAEDIEPQVTWGTSPEMVLNIGGKVPNPAEEADPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKGRKKAGNVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17218..17472
                     /locus_tag="WJL14_000134"
     CDS             17218..17472
                     /locus_tag="WJL14_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655266.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17534..18175
                     /gene="leuD"
                     /locus_tag="WJL14_000135"
     CDS             17534..18175
                     /gene="leuD"
                     /locus_tag="WJL14_000135"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ7655267.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETFAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18350..18610
                     /gene="dcm"
                     /locus_tag="WJL14_000136"
     CDS             18350..18610
                     /gene="dcm"
                     /locus_tag="WJL14_000136"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7655268.1"
                     /translation="MQQIKFIDLFSEMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFA"
     gene            18656..19222
                     /gene="dcm"
                     /locus_tag="WJL14_000137"
     CDS             18656..19222
                     /gene="dcm"
                     /locus_tag="WJL14_000137"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7655269.1"
                     /translation="MARILKAKKPKGFILENVEGLVTHDRKDSTQKIGRTLTVILETL
                     EALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSFETSPSPKLKNILESGLPT
                     ESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIELKGAVTEEEKQLLNILLKE
                     RRKKMGFRNRHRLDGWDAFDKSANFNFL"
     gene            19185..19619
                     /locus_tag="WJL14_000138"
     CDS             19185..19619
                     /locus_tag="WJL14_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7655270.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19635..19796
                     /locus_tag="WJL14_000139"
     CDS             19635..19796
                     /locus_tag="WJL14_000139"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7655271.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            19797..20366
                     /locus_tag="WJL14_000140"
     CDS             19797..20366
                     /locus_tag="WJL14_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ7655272.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20400..21470
                     /gene="leuB"
                     /locus_tag="WJL14_000141"
     CDS             20400..21470
                     /gene="leuB"
                     /locus_tag="WJL14_000141"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ7655273.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKEYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATVLSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTGD
                     IYEEGTKLVSCSEMGDAVLATL"
     gene            21633..22196
                     /locus_tag="WJL14_000142"
     CDS             21633..22196
                     /locus_tag="WJL14_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ7655274.1"
                     /translation="MKKIIIAALAAAAIGTASAATYKVDEYHANARFSIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMLKTEVCGGDFSTTIDRT
                     KWGMDYLVNVGMTKSVRIDIQIEAAKQ"
     gene            22657..24054
                     /gene="aspA"
                     /locus_tag="WJL14_000143"
     CDS             22657..24054
                     /gene="aspA"
                     /locus_tag="WJL14_000143"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ7655275.1"
                     /translation="MTVRIEHDLLGDREISAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPFKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(24264..25139)
                     /locus_tag="WJL14_000144"
     CDS             complement(24264..25139)
                     /locus_tag="WJL14_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7655276.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(25141..25863)
                     /locus_tag="WJL14_000145"
     CDS             complement(25141..25863)
                     /locus_tag="WJL14_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ7655277.1"
                     /translation="MDKDLYAVLGVSPQAGANEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYATHTEYLIKTFWRTFWLYLLGALTALLGIGVLIIIATNVWYF
                     YRIIVGFIRFNSGRAVAPEKWI"
     gene            complement(25885..26364)
                     /gene="ybaK"
                     /locus_tag="WJL14_000146"
     CDS             complement(25885..26364)
                     /gene="ybaK"
                     /locus_tag="WJL14_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ7655278.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLDIIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            26439..26801
                     /locus_tag="WJL14_000147"
     CDS             26439..26801
                     /locus_tag="WJL14_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7655279.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAMKKDAATA"
     gene            26836..27699
                     /locus_tag="WJL14_000148"
     CDS             26836..27699
                     /locus_tag="WJL14_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ7655280.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVDAEL
                     EASAEPLAAQEPNLAFAAQQDSDGICSIALPHGGILRIAPETALPPYDAAAESAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEAGILLTADGEEAGIVLDSVKDSENFTALAVIKFSAAQKTLSAPNGGSFKA
                     VHLFFKTENAE"
     gene            27792..28751
                     /gene="ttcA"
                     /locus_tag="WJL14_000149"
     CDS             27792..28751
                     /gene="ttcA"
                     /locus_tag="WJL14_000149"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ7655281.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(28845..31784)
                     /locus_tag="WJL14_000150"
     CDS             complement(28845..31784)
                     /locus_tag="WJL14_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ7655282.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFM
                     IVDEPHKFPTRDSAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSDGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRAEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENTAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMNYREFLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAV
                     GHSENGY"
     gene            complement(31797..33488)
                     /locus_tag="WJL14_000151"
     CDS             complement(31797..33488)
                     /locus_tag="WJL14_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7655283.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGSYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
BASE COUNT         8122 a         8907 c         8453 g         8207 t
ORIGIN      
        1 ggcatttcca aagggcattt taagctgtat ttgaaagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttctccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcagaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattattgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tgttgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggcggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttgtggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccac cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagcgcg caattaggtt
     2641 tcaccggacg cgtattgata cccgtagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggagca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgcc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccggg ggcggtaaca cattcggcag cttagacggc ggcacaggta tgggcggcag
     3121 catcgtcaaa atggcggtag aaagccaatg ccgtgcggaa ttgaacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccaacca gctgaccggc aacgatgtga tgcagatgct
     3301 ggatccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcccccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 gtcttatccc gaattggcgt ttgaaatcat gcgcctgttt gttacggata ttccggaaga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttatcca acggccccac
     3781 tttggcattc aaagacatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggcgt gaacgtattt atgttgtcgc ccaacggtaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgagaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggacattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttc tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac acatcgccaa
     4261 acagatgggt ttgcctatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggtg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtcg atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggtgaaac
     4681 ggtggtttgt ttggaaaccg cgttggcggc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgtc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcaacg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtatgggcgg cattccgtcc gaacttaaga ttgaaagaaa atgaccgtac
     4921 ctatgcagga aacccatttt tccatactgc tggacgaact ggcggcaaaa caggaggcag
     4981 ccatcgcccc ccacctgctt gccgacggaa ccaaggtatg gatacgcaaa gcaggcaggc
     5041 acaatgcgcg atggcgttac gcgctgctcg gtatggttgc ccgatatttg aaactggggg
     5101 tgttgaagcc ggttcccagc ctcggcggcg agcctgccat tgcaaccgaa tcaaaacgcc
     5161 tgtacgaatt gcgcgcggcg gggatagccg ttcccgaatt gctcgccgtc aggaaaaatg
     5221 cccttatgtt cggaaatttg gaaggtatcc cgctcgatac gcaaatccgc caagaagccg
     5281 aagccggaaa ggcggacgct tggcttgccg gtttggaagc cattgcacgc gtgcataaaa
     5341 aacagcagtt cctcagtcag gcgtttgcac gaaatatgat gtcggacgga aaaaacatca
     5401 gctttttgga ttttgaagac gacccttccg aggtcctgac catagcgcaa tgccaagccc
     5461 gcgattggct ctgctacatt cattcgaccg cgctgatatt gaaaaacggc ggactgctgg
     5521 aagcggctgc ggaaaaatgg ggcggcgtat tgtcagatca gcctgccgaa atacaaaagc
     5581 tgattgccgc caccgtcaaa ccgatcctcc cgatacgcag gctggaacac ccccgctggg
     5641 gacgggacgc gctcaggctg gcagcctcga tttccctgat ttcccttgcc gatatgccgc
     5701 cgtaagccgc cgcccgcccc acgatgccgt ctgaagccct tcagacggca tataaccaac
     5761 gcgcatcaag cattgtcgca accatccgtt tcgtttagaa ttttagcttt accccgaatc
     5821 gggcagacat tttcactctg ccgcacatcc cgaacaagca ctatccattt cttttactga
     5881 caggaaaccc aaatggcagc attcaatacc caaaaagtat tgtccgtaca ccactggaca
     5941 gacgcatatt ttacctttac ctgcacccgc gacgaatcgt tgcgctttga aaacggacag
     6001 ttcgttatgg tcggattgat ggtggacggc aagccgctga tgcgcgcata cagcgtcgcc
     6061 tccgccaact gggaagaaca cctcgaattt ttcagcatta aagtccaaga cggtccgctg
     6121 accagccgcc tgcaacacct caaagtcggc gacgacgtgt taatcagcaa aaaaccgacc
     6181 ggaaccctgg ttgccggcga cctgaatccg ggcaaacacc tttacttgtt gagcaccggt
     6241 accggcatcg ctcctttctt gagcatcacc aaagaccccg aaatttacga gcaatttgaa
     6301 aaaatcatcc tcgtacacgg cgtgcgctac aaaaaagatt tggcgtacta cgaccgcttt
     6361 accaaagaat tgcccgaaca cgaatacctc ggtgacttgg ttaaagaaaa actgatttac
     6421 tacccgattg tttcccgaga agaattcgaa caccacggcc gcctaaccga cctgatggtg
     6481 agcggcaaac tgtttgaaga catcggcctg cccaaaatca acccgcaaga cgaccgcgcg
     6541 atgctgtgcg gcagccccgc gatgctgaaa gacacctgca aagttttgga cgatttcggt
     6601 ctgaccgtct ccccgaaaac aggcgtgcgc ggcgactacc tgattgagcg tgcgtttgta
     6661 gatcaataag ctgtttgaaa atcccgtctg aacggcatca gcgataaaaa aacccgaatc
     6721 ctgccaatca ggattcgggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 tttccgcgca ggctaggatc caaacttatc cgtttcatta tagtggatta acaaaaacca
     6841 gtacagcggt gcctcgcctt gccgtactat ttgtactgtt tacggcttcg tcgccttgtc
     6901 ctgatttttg ttaatccact atagtatctg aaaataaaag agaaaccaaa catatagatt
     6961 cccgcttgcg cgggactgac gacggtgttc tgcgtagcca aagcagaaaa acatccttat
     7021 tgtcatttca tcatgatata acaataagga tgttaaaaat tagttatcat tatatgcatg
     7081 atatataaga aaaattgtcc caataagaaa tgttatgcag aaaataaaat gaaaaggatg
     7141 gttttcattg aaaatggttg ttctgccacc gtcatacaca gaaccggtat ttggaaaaaa
     7201 aataatccat attgacatca ttattccatt tatcgcataa atccactttt taattttttt
     7261 tcctcattaa tataatctct tatttaataa aatcaaatac ctagcaattc gatctcaaat
     7321 tttaaacgcc aacgtgtttg agcggtaaca atgcatactt taagtttgta atccttcatc
     7381 gtctcatttt tgttttaatc gactatacgt ctaaagcaac gccgtccgtc attgttcgga
     7441 cggcattttt tcagacggca ttttcccccg tttgtgaagg cgcaaggcat tgcccaaaac
     7501 cgccagcgaa ctgaaactca tacccagtgc ggctatccac ggttggacat agcccaaaac
     7561 ggcaagcggt acggcaatga tattgtacgc gcccgcccat atcaggtttt gccggataat
     7621 ctgccgggtg cgccgcgcct gatcgagcag gtgggcgacg gtacgcaaat cttcgttcaa
     7681 taacacaatg tccgcgccgt ccctcgcaat atccgtcccg cccgctgcgg cggcggatac
     7741 gtctgcctgc gccaaaacgg gcgcgtcgtt gatgccgtcg cctatcatca gtactttttt
     7801 cccttctttt tgcaaggctt tgacgtattc cagtttgtcc tcgggcatgg cttgggcgcg
     7861 gtagtgcgtg atacccaggg cgcgggcggt ttcggcaacg gcggtttcgc ggtcgccgct
     7921 gaggatgtgc agggtcaggt ttttgcctgc caactgccac accgcctccg ccgcgctgtc
     7981 tttcaagggg tcggtcaggt agaacacggc ttggaaaccg ctttgactgc cgaggtaaac
     8041 cgcgctgccg ccgccttctg tttgcggttc tttacctgaa atttcggcga cataggatgc
     8101 cctgcccaat gcccaaacct gtgtttcgcc gttgacggtc agttgcgcgc ccacgccttc
     8161 gccgatgcgg ttgaggcgtt gtttaatagc aatgtcgggg acgctgccgt ctgaaacacg
     8221 gtgtttgagg atggcgcggg caaggggatg ttcggactgt tgttctaaag cctgcgccac
     8281 cgcgagaaca aaggcttcgt ctgtgcctct caacaatgag atacggcgga cggcgggatt
     8341 gccttgggtc agcgtgccgg ttttgtcgaa aataatgtcg gtggtttggg cgagcgtttc
     8401 cagggtttgc ttaccgccga ttaaaatacc ttcacgcgcc agcgtaccgg tagaagctgc
     8461 cagcgcggtc ggcgtggcaa gcgataaggc gcaggggcag gtaatgacca gcagggcgac
     8521 ggtaatccac aatgcggtgt gcgcgtcggc gtacagcgtc cagccgatga agacggggac
     8581 ggcaagcagg agttcgccga atatgaaaga cgaggcgtat tgttccgcca actcggcagt
     8641 gcgcggtttt tgcgctaagg cgcggtcgag caggcggacg atgtgcgaca gtcgcgtgcc
     8701 accgccggtg cggtcggtgc gtataatcag ggggctttgc gtgttgagtg tgccggcggt
     8761 tactttttca gacggcattt tggcgacggg caggctctcg ccggtcagca tagattcgtt
     8821 gacggcactg cttccttcca gcaccgtgcc gtcaacgggg atggtttcgc ccggtttgac
     8881 cagcacgata tcgcccgcct tcaatttgac gacagctgcc tcgcaggttt cctgcgtatc
     8941 ggggtaatcg ggcatatgat ggcaaaacgc aggaatcagc ttcaccagcc tctcggcggc
     9001 atcgcctgcc ttacggcggg caatgtgttc cataaagcgt ccgcccagca ggaaaaacag
     9061 cagcatcgcg atggattcga aatacatccc ctgccccgca tttgtcgcaa ggctgtaaac
     9121 gccggcgata aaggtcatga tgatggcgac ggtaatcggc gtatccatgc cgacgcggcg
     9181 gtttttcaag tcgcgcagcg cgccttgata aaacgggacg gcgcaataga atacgacggg
     9241 cagcaccatt aaaaagccgc cccaatggag gatttgcagg aaatcgggtt cgatgtcgct
     9301 gccgtaaagg taggtcggca gcgcgaacat catcgtctgc atcatcccca gcccggcaac
     9361 ggcgaggcgg acgatgtatt gtttgcgttc tttttggttg gcggcttcga ttttttgcgc
     9421 gtcatagggt gcggcggtgt agcctatctg cctgattttc aacagaatgt cggaaaggcg
     9481 gattttgccg tcgtcccaga cgacgcggca gcggtgcgtg ctgtaattga ggtcgatgcg
     9541 gacgatgccg tctgtacgca aaagctgctg ttcgatcagc cagacgcagg cggcgcaggt
     9601 gatgccgccg agcattaaaa ccgcctcgcg cgtgccgccg tgggtttcca caaagtcgga
     9661 ctggacttcg ggcaggtcgt acaggcggat ttggtcgagg atttcttggg gcggcagctc
     9721 ggttttttgc gcgtcggcgg tgcgttgttt gtaataactg cccaagcccg cgtcaataat
     9781 gctttgtgcg accgcctgac agccggcgca gcaggtttcg cggtcttcgt tttcgtaacg
     9841 gacagtcagg tggaggtgtt cgggaacatc cagaccgcag tggaaacagg tttttttcat
     9901 ggcatttcgg tttcgtctgt gtttggtgcg gcggcacaag actcggcaat ggcttcgcgc
     9961 agtgcgtcta taccggtatt ttcagcaacg gaaatgcgga cggcggcaat ttttcccgca
    10021 gcgtcgcgcc atatgcccgt gttttgttct tcagacggca gcaggtcggt tttgttgtac
    10081 accttgatgc acggaatatc gccggcatgg atttcttgca gtacgttttc cacgtcttca
    10141 atctgctgtc cgctgttcgg agcggcggca tcgacgacgt gcagcagcac atcggcttgc
    10201 gcggtttctt ccagcgtggc ggaaaaggcg gaaatcagtt tgtgcggcag atcgctgacg
    10261 aatccgacgg tatcggtcag gatgatgctg cattcgggac tgatgtacag ccgccgcgcc
    10321 gtggtgtcga gtgtggcgaa aagctggtct ttcgcatata tgcccgactt ggtcagccgg
    10381 ttgaacaggc tggatttgcc gacattggta tagccgacca gcgcaaacgt tttgattgtg
    10441 cccgattcgc gggacttgcg gcgcagggcg cgctgttttt tgaggttggc aagctgtttt
    10501 ttcaaggcat tgatccgatg ggcgatcaat cggcggtcgg tttccagttt ggtttcgccg
    10561 gggcctttca tgccgatacc gccgcgctgg ctctgcagat ggccgtaacc gcgtatcaag
    10621 cgtcccgcca aatggctcaa ttgcgccaac tcgacttgca gcctgccttc ctgcgtgcgg
    10681 gcgcggcgag cgaaaatcgc cagaatcagc cctaccctgt ccaatacgcg gcatttcagt
    10741 tctttttcaa ggttgcgttc ctgcgtgggc gtgagttcgt ggttgaatac gaccaaatcg
    10801 atgccgtctg cggcaactgc ttctgacagc tccgccgcct tgcccgtgcc gacaaacagc
    10861 gcggtgtgcg gacggtcgcg tttggcagtc tccacgcgca cggaatcgcc gcccgccgct
    10921 ttgaccagct cgacagcttc cgccaatgcc gtctgaaaac cgttcagacg ggcggcacta
    10981 ctgcccgtac catctttgtc caacattacg cccaccagca tgacgcgttc gggttgcgct
    11041 tgggtactgc cgttgccgcc tgttctgcct gtcatgtatt caatcctgta aataaagggt
    11101 tacgccgcgt tccgggctgt cttccggtgc aagcgtgatg ctgctgcccg ccattccgcc
    11161 aacgctgaag acgatgccgc ccgcctcgtg ccttcctatg gacaggcggc tgccgggtcg
    11221 ctgccggtag gtataacggg tacacgcttc caaacaccag cgtccgcgcc agtggatgaa
    11281 atatttttcg ccttttctaa ccgcaagtgt ctcgccgccg tttacctcaa aataagggta
    11341 atcgggtttt cgggttctaa actgccattt cgcctgcgcg cgccgtccgt tgcggacata
    11401 gtcgaatacc gccgtataaa gcgtaccgta ttccaaaggc ttgagcggga aaagcgcgaa
    11461 ttggtacgcg gtcagcctgc cgttggggtc gttgccggcg gttaaaaccc tgacggggcg
    11521 gatttcgttt ttaccctgat acagcttgaa acttttcatc gtaattttgc ctgccgcctc
    11581 ggaaaaatca atgctggcag gattgcccgt gatttcatat tccggcacgg ggtctggacg
    11641 ctcgccgtgg aaataaggca gtgcgccgtt gccgacggga taggctgtat agagcagctc
    11701 ctgtgcgggc atggcttcgt cggtgtacac gaccgcaccg ttatggcagg cgttgcggta
    11761 atatttccgt cctgcttccg gctgatttct gccttgggcg caatgccgct caaacctgcc
    11821 gttgccctga ttgaatacga gaacggtttt accgttttcg cgcacaaatg ccgctcctgc
    11881 ctcatccgta tggcggtcaa gtagggaaag gcggtggtag attgcgctca tcaatccgtc
    11941 cacttggcgt tgctgcgtgc ggatgtcgct gtcggacgat tcggcggctt cctcttccgt
    12001 gctgatgttt tcatgcacgc cgttgtagag atacccggca aggcgtgtgc gttcggtcag
    12061 cttttgtgcg gtgtagtgcg gattgtcggg atggtgttcg ccgtgtccgt cttcgggatt
    12121 gagcgtgaga tagcgtgcgt gcctgcgggc ggaattttcc aaaaccggcg cgtgtgccag
    12181 cttgtgcaaa ccgatttggg cgcggatgcg gttgaggtag tgcagcgcat cgccgccgtc
    12241 cctgatttgc ggtgcggacg gatagacaag ttcgcccgcg ggcagggatt ggttttgggt
    12301 atgatagaaa acgcctgccg ccgaacccaa caggagcagc cgaataaaaa gggatttcat
    12361 aatgccgtcc tcccgacgcg gcaacggggt gttttatcaa aacggcaaac ttgccaatgc
    12421 ggtttccgat tgcagattgc caaaccggca aacctttccc cgtgccgatg ccgaacctga
    12481 tgccgtctga accttcagac ggcatcggat gtttatttgt ccacccggat gggtgcaggt
    12541 tcgtattgtg tcgattcgtc gccgtaatac agcacgccga gtttgatggg gattctgccc
    12601 tgtgatttgc ggtgggcatt ggaatccctc agggaatagg cacaaccgca atattcctgc
    12661 tggtagaagt tttcacgttt gctgatttca atcatgcgcg cgctgccgcc gcctttgcgc
    12721 cagttgaaat cccaatacac cacatcatcg taaggcgcgg cggcgcggtg tccgcagtcg
    12781 ttgatttgcg ccatattttt ccagcgtgaa atgcccagcg aactggtaaa gacggggaac
    12841 ccgtgttcat gcgcgtattg cgcggctttt tcaaaacgca tatcgaaaca catggtgcag
    12901 cggatgccgc gttccggctc aaactccatg cctttggctt tggcaaacca ttctttgcgg
    12961 tcgttttcgt agtcgtcgtc tttatcgatg aaaggaatgc cgaacttttc cgcaaagcgc
    13021 acgttttcct cttttcggag catatactct ttgtgcggat ggatattggg attgtaaaaa
    13081 taaatggtgt agtcgatgcc ggaggcaagc atcgcctcca tcacttcgcc gctgcacggg
    13141 gcgcaacagg aatgcagcaa gactttttta tgtccgccgg gcgggacgag tatggggcgg
    13201 tcgatgtcgg taacgatgcg tgttgtgtcc atagtttcaa atagatgttc tgtttggtgc
    13261 aaacggctat tttatcagag ccgccgcatg taaaacaggg ggtcagggca tcagcccgag
    13321 ctgcctgaac gaacggacgg tttgcgccgt tacgataaaa tggtcgagca gcgacacatc
    13381 gaccagcgac attgcctgtg ccagccgcct tgtgaacatg atgtcttcct gcgaaggttc
    13441 gggcgagccg cccggatggt tgtgcgcaat aatcaggctg tcggcatatt cgtccaatgc
    13501 cagtttgacg atttcgcgga tgtaaatcgt gttttccgca accgtaccgc gcgacagctc
    13561 tctgaccgca atcagttggt tttggcggtt cagcagcagc gcgacgctga cttcgacttt
    13621 ttcctgcccc aaatgaaagc gtaaataatc ggccaccgta tccggatcgg acaggacgat
    13681 gctctcctgc aattcttccg ccaatatccg ccgcccgatt tccctgacca cggcaaactg
    13741 tgtgaaactt gccgtcccca tccctttgta tgccgacagt ttgccgacct ccgcgctcat
    13801 cagcctcccc aaactgccga actcctgcag caaataacgc gccaaatcga ccgcactcat
    13861 tccgcgcgtg ccgacgcgca gcaggattgc caaaagttcg gcatcactca acgccgccgc
    13921 cccgcgttcc aacagctttt ccctgggcct ttccccctcc ggccattgct tgatgctcat
    13981 gttttccctt ttttaaatag tctgagatgc cgtctggaac aaatgttccc acacgccaaa
    14041 tatccgaaat gccgtctgaa ccggatttcc gcttcagacg gcatctgtat ttcaaaacag
    14101 tcttacgccg tttcttccaa ctcgatggac gccgccagaa gcgacaggcg tgccattacg
    14161 ccgtaaacgt agaggcggtt gtgttcgttg tccacgtcgc agtctttttc agagcgcggc
    14221 atacccagcg agtcccattg ttcgaatacg cgcttgcagg cttcgggcgc ttcttgggaa
    14281 ttatcgccgt taccggtagg aatgctgtta gacagcggaa caaacaccat accgccggcg
    14341 ttgaggtttt cgtccgcacc gcgcccttcg tgtacgcgga aaaagccgcc gatgacgaag
    14401 cggtccatca tatacacgac gggttcgcac accgcgccgt ttaaggtttc ataagtataa
    14461 atcccttctt ggacaatcac ttcgctgact tccaagcctt ctttgacttt cgccatttta
    14521 ttgcggtttt tacggttcaa tccgcgcact tcgtcggcgg atttgacgct catcacgccc
    14581 atgccgtaag tgccggcatc ggctttgacg atgacgaaag gtttgtcggt aatgcccgat
    14641 tcgtcgtatt tggcttgaat tttcgccagc acacgttcta ccgcttccgc caacgcgtct
    14701 tcgccttcac gcccttggaa gtccaaaccg ccgatttttt caaaataagg gttaatttgc
    14761 cattcgtcga tgccgattaa cttggcaaat tcggcggcaa cttggttgta cgcgccgaaa
    14821 tgatttgttt tgcggcgcgt cgtccaaccg ccgtgcaacg gcggcaaaac ggtttggctg
    14881 atgcctttga ggatgtcggg aatgccggcg gacaaatcgt tgttcaacaa aaccacgcaa
    14941 ggcgaaaagc cgtctgcaag atggacgcgg tcgcgggtac gcaataaagg ttccaacagg
    15001 attttgtcgc ccaatgcggt ttcaaattcg gtcggttcgg ttacttccgg attcaggctg
    15061 cccaagcgca cttcataccc tgccgaacac aaaatctcgc cgagggcgta aacgttttgc
    15121 aggtaaaacg tattgcgcgt gtggttttcg ggaataatca atacggattt cgccgtttca
    15181 caggcgcgtt gcaccgcatc ttgcgcggca accgccgcca gcgggataaa gttgggattc
    15241 agattattga agccgccggg gaacaaattc atatcgatag acgaaatttt gtaaccggca
    15301 ttgcgtatat cgaccgaacc gtaaaacggc gggcggtgta cgctccactg cgtgcggaac
    15361 cacgcctcga ttttggcgtg attggacagg atttttgcct caaacgcctg aagttgcgcc
    15421 gaatgttcgg gcgacatcac cggtaatttc atctttcgtc tgcctctgtg tgttttttga
    15481 caagatgcag atgataatgc ctttgaaaga atgacgacaa gtattctttc gtatcgaatc
    15541 atgctttaga tataactgtt tactgaaatg gacaatatgt ctaatttttt cgttaaaatt
    15601 gtttgcaaaa taacaccggc tcaaaaatta gacaaaattt gttgcgcggt ataaagaata
    15661 cgtctaaaat ccgcccaatc cgcattacca tttatccaaa agaacaacat catgacagca
    15721 caaaccctct acgacaaact ttggaacagc cacgtcgtcc gcgaagaaga agacggcacc
    15781 gtcctgctct acatcgaccg ccatttggtg cacgaagtta ccagccctca ggcatttgaa
    15841 ggcttgaaaa tggcggggcg caagctgtgg cgcatcgaca gcgtcgtctc cactgccgac
    15901 cacaacaccc cgaccggcga ttgggacaaa ggcatccaag acccgatttc caagctgcaa
    15961 gtcgatactt tggacaaaaa cattaaagag tttggcgcac tcgcctattt tccgtttatg
    16021 gacaaaggtc agggcatcgt acacgttatg ggccccgaac aaggcgcgac cctgcccggt
    16081 atgaccgtcg tctgcggcga ctcgcacact tccacccacg gcgcattcgg cgcactggcg
    16141 cacggcatcg gcacttccga agtcgagcac acgatggcga cccagtgcat taccgcgaaa
    16201 aaatccaaat ccatgctgat tgccgttgaa ggtcgtctga aacctggtgt taccgccaaa
    16261 gacgtggcac tttatatcat cggccaaatc ggtacggcag gcggtacgag ctacgccatt
    16321 gagtttggcg gcgaagccat ccgcagcctt tctatggaag gccgcatgac cttgtgcaat
    16381 atggcgattg aagcaggggc gcgctccggc atggttgccg tcgatcaaac caccatcgac
    16441 tatgtcaaag gcaaaccttt cgcacctaaa ggcgaagcgt gggacaaagc cgtcgaatac
    16501 tggcgcacgc tggtgtctga tgaaggcgcc gtattcgata aagaatacca cttcaaagcc
    16561 gaagacatcg agcctcaagt aacttggggc acgtcgcccg aaatggtttt aaacatcggc
    16621 ggcaaagtgc cgaaccctgc cgaagaagcc gatccggtca aacgcagcgg catggaacgc
    16681 gcactcgaat atatgggctt ggaagccggt acgccattaa atgaaatccc tgtcgacatc
    16741 gtattcatcg gttcttgcac caacagccgc atcgaagact tgcgcgaagc cgccgccatc
    16801 gccaaaggcc gtaaaaaagc cggcaacgta cagcgcgtgt tgatcgtccc cggctccggc
    16861 ttggttaaag aacaagccga aaaagaaggc ttggacaaaa ttttcatcga agccggcttt
    16921 gaatggcgcg aaccgggctg ctctatgtgc ctcgccatga acgccgaccg cctgactccg
    16981 ggacaacgct gcgcctccac ttccaaccgt aactttgaag gccgtcaagg caacggcgga
    17041 cgtacccacc tcgtcagccc cgctatggca gccgccgccg ccgttaccgg ccgctttacc
    17101 gacatccgca tgatggcgta agtattttca ggcggtcttt aagaatttga cgcggccgtc
    17161 tgaaatatgt cccgtaaggc agaaacacct ttaatattca aaccaaggag tttaaccatg
    17221 aacaaacttt tcattaccgc cctgtccgcc ctcgccttgt ccgcctgcgc cggcacttgg
    17281 gagggcgcga aacaagacac cgcccgcaac cttgacaaaa cacaggccgc cgccgaacgc
    17341 gccgccgaac aaacaggcaa cgccgtcgaa aaaggctggg acaaaaccaa agaagccgtc
    17401 aaaaaaggcg gcaatgccgt cggacgcggc atttcccatc tcggcggaaa aatcgaaaac
    17461 gccaccgaat aaccgattcg cgatgccgtc tgaaaccgct tcagacggca tcgtcagcac
    17521 aagacgcaaa cccatgaaag cctttaccaa aattaccgcc atcgtcgccc cgctcgaccg
    17581 cagcaacgtc gataccgatg ccatcatccc caaacaattt ttaaaatcca tcaaacgaag
    17641 cggcttcggc ccgaatgcct ttgacgaatg gcgttacctc gaccacggcg aaccgggtat
    17701 ggacaacagc aaacgcccgc tgaaccccga tttctccctg aaccagccac gctatcaagg
    17761 cgcacaaatc ctgttgacgc gtaaaaactt cggttgcggc tcttcacgcg aacacgcccc
    17821 ttgggcattg gacgactacg gcttccgcgc cgtcatcgcc cccagctttg ccgacatctt
    17881 cttcaacaac tgctacaaaa acggcctttt gcctatcgta ttgaccgaag aacaggttga
    17941 ccggcttttc aaagaagtcg aagccaacga aggctaccgg ctctccatcg accttgccgg
    18001 gcaaaccctg actacccccg gcggcgaaac attcgcattc gacattaccg aacaccgcaa
    18061 acactgcctc ttaaacggct tggacgaaat cggcctgacc ctgcaacacg ccgacgaaat
    18121 tcacgccttt gaagaaaaac gccgccaaag ccagccttgg ctgtttaacg gttaaaacct
    18181 atgccgtctg aaggccgttc agacggcatt cctgataaca tataattgtt gcggctaatg
    18241 taaacagagg ggcgataaat tgctttcatc ttttaagcgg gcttagcgac actctaaatt
    18301 aatgcaataa tgcaactctc catttcaaat aaatttttag atttttaata tgcaacaaat
    18361 taaatttatt gacttatttt ccgaaatgag tggcatcagg aaaggattcg aacaagcctg
    18421 tcggaaacag tcggttgctt gcgaatgtgt ttttacttca gaaatcaaac cggcagcttt
    18481 ggaagtattg aagcaaaact accctgatga agtgccgtat ggagatataa cgaaaattga
    18541 aacaggggat attcccgatt ttgacatcct gttggcaggc ttcccttgtc aggctttttc
    18601 tttcgcctga aaaagattag gctttgaaga tacacgggga acgcttttct ttgatgtggc
    18661 aaggatttta aaggcaaaaa aaccaaaagg ttttattttg gaaaatgtgg aaggattggt
    18721 gacacacgat agaaaagatt cgacacaaaa aatagggcgc accctgaccg ttattttgga
    18781 aaccttggaa gcattgggct actatgtttc ttggaaagtt ttaaatgcaa aagatttcgg
    18841 cattccccaa aacaggaagc gcatttatct gacaggcagt ttgaaatcca aacccgactt
    18901 atctttcgaa acaagcccaa gtccgaaatt aaaaaatatt ttggaatcgg gactgcctac
    18961 cgaaagcagc cctttcatca aaaaattgct aaaaaaattt cccccgtccg aactgtacgg
    19021 aaaatcagta aaagacaaac ggggagggaa aaacaatatt cacagttggg atattgaatt
    19081 aaaaggcgca gtaaccgaag aagagaagca attgttaaat atccttctaa aagaacggag
    19141 gaaaaaaatg ggcttcagaa atcggcatag attggatgga tgggatgcct ttgacaaaag
    19201 cgcaaatttc aactttctat aaacaccccg atctccaaaa tattttggac agcctgacag
    19261 ataaaggcta tttggtttta gagcatccga aacaaaaaat tggcgggcaa agaatcaaag
    19321 atgaatccct gcccaaaggc tacaatattg tttcaggtaa aaaatctttt gaaatcaata
    19381 aaatattaga tccaaacgat gttgcgccaa ccttggttgc aatggatatg gagcaccttt
    19441 tcgtcgttga caacggcggt ttgcgtacac ttaccggaaa agaagggttg cgcttattcg
    19501 gctatccgga cgattattcg tttgatattc ccaaaaaaga cagatgcgat ttattgggta
    19561 ataccgttgc cgtccctgtg attaaggcgg tatctgaaag acttctgcat actttataaa
    19621 ccgacaggga taaaatgata aaacttactg cacaacaaat atttgataag ttgctggatg
    19681 aagaaaaaat cttatcggcc aatggtcaaa ttagattttt cttaggcgat gtggatatta
    19741 tcgtcaaaca aaaagatgtt gtcggcaaca tcattcagga atggctcggc ggatgattga
    19801 gaaaaaggga aattgaattt gatgtttcaa ccaataccca aatgcctccc gatttctttt
    19861 taaataaaaa agaccgcagc agagaattgt tggaagtaaa ggcgttcaac cgaaatgccg
    19921 gcccgggttt tgatattgca gattttaaaa tgtattctga tgaaatcatt cataagccct
    19981 atatgctgga tgtagactat ttaatattcg gttacgatat ggacgacaac ggcaatgtaa
    20041 ccatcaagga tttatggctt aaaaaagtat ggcaaattac gagaagcatg gatgggtggg
    20101 caattaatct tcaagtcaaa aaaggcgtgg tgcataaaat ccgcccgggt gtttggtaca
    20161 gcataaataa aaagaatatg cctatgtttg aatgcttgga agattttgtt tccgcaattg
    20221 aagaaaccgt ttatcaaaac ccggcaacac ggcataacgc atccttatgg aaaaagaaat
    20281 ttgaggaagc ctataaaaaa cactacaacc gatcgatttc catacctcgt tggcacgaaa
    20341 ttgcacacaa atacaaaaag aaataaccct tcaaccatca acccataagg aaacttgaaa
    20401 tgaccaaaca tatcgccatc ctccgaggtg acggcatcgg cccagaaatc gtcgccgaaa
    20461 ccgtccgcgt actcgacaaa cttatcgccc aaggcttgga cgccggctac gaatacgcac
    20521 ctttgggcgg cgaagcctac gacgaatacg gtcatcccta tcccgaattc acgcaaaacc
    20581 tttgccgcaa agccgatgcc gtcctgcttg gtgccgtcgg ttctcctcaa tacgacaacc
    20641 tcgaccgtcc gttgcgccct gagcgcggcc tgttggcaat ccgtaaggat ttgaacctgt
    20701 ttgccaattt gcgtcctgcc gttttatatc ccgaattagc caacgcctcc acgctgaagc
    20761 ctgaaatcgt tgccggtttg gatattttga ttgtgcgcga actcaccggc gacatctatt
    20821 tcggcgaacc gcgcggcatc cgcgttctag aaaacggcga acgcgaaggc tacaacacca
    20881 tgaaatacag cgaaagcgaa atccgccgta ttgcccacgt cgccttccaa tccgcccaaa
    20941 aacgcagcaa aaaagtctgc tccgtaggca aagccaacgt tttggaaacc accgaattgt
    21001 ggcgcgaaat ctttgaagaa atcggcaaag aataccctga tgtcgagctt tcccatatgt
    21061 acgttgataa cgcagccatg cagttggtac gcgcgcccaa acaatttgac gtgattgcca
    21121 ccggcaacat cttcggcgac atcctctctg acgaagcgtc tatgctgacc ggctctatcg
    21181 gtatgttgcc ttctgcttct ttggatgaaa acggcaaagg cctgtacgaa ccttctcatg
    21241 gctccgctcc cgatattgcc ggtcaaaaca aagccaatcc gctggcgacc gtattgtctc
    21301 ttgccatgtt gctgcgttac agcctgaacg acgaagcacg cgcgcaacaa gttgaaaacg
    21361 ccgtccaaaa agtgctgcaa caaggcttgc gtaccggcga catttatgaa gaaggcacga
    21421 aactcgtttc ctgctccgaa atgggcgacg cggtactcgc caccttgtaa aaaaacaaag
    21481 cgcggatcac accgcgcttt ttcatctgcc cttttccgct cgccccgttc gtcaaccgcc
    21541 tgcaaaccca ccgtttaccg gcaggaaatg ccataacccc ccgcctatgt taggataaac
    21601 gcctttaccg gcccacacaa aggattatca tcatgaaaaa aatcatcatc gccgcgctcg
    21661 cagcagccgc catcggcact gcctccgccg ccacctacaa agtggacgaa tatcacgcca
    21721 acgcccgttt ctctatcgac catttcaaca ccagcaccaa cgtcggcggt ttttacggtc
    21781 tgaccggttc cgttgagttc gaccaagcaa aacgcgacgg taaaatcgac atcaccatcc
    21841 ccgttgccaa cctgcaaagc ggttcgcaac actttaccga ccacctgaaa tcagccgaca
    21901 tcttcgatgc cgcccaatat ccggacatcc gctttgtttc caccaaattc aacttcaacg
    21961 gcaaaaaact ggtttccgtt gacggcaacc tgaccatgca cggcaaaacc gcccccgtca
    22021 aactcaaagc cgaaaaattc aactgctacc aaagcccgat gttgaaaacc gaagtttgcg
    22081 gcggcgactt cagcaccacc atcgaccgca ccaaatgggg catggactac ctcgttaacg
    22141 ttggtatgac caaaagcgtc cgcatcgaca tccaaatcga ggcagccaaa caataaatct
    22201 tgatgcgaat cagcacaaat gccgtctgaa cgtccttcaa acggcatttg tgccgtcttc
    22261 atcaaaccgc cccgccaacg gtaagccgat atggcggagc acaatccgcc gaaagatatt
    22321 tcagcggcag gcagggcttg ccggaaagtc cggaaagtat agtggatcaa caaaaaccag
    22381 tacggcgttg gctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccgag
    22441 tgaatcggtt ccgtactgtt tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
    22501 taatccacta taacacccga aaacaacgtt tcccgttttc agacggcata cgatatggac
    22561 aatttcatcc ggccggtata gtgttcgact tcatgcgtta tccttccgat accgcatttt
    22621 ttcaatatca attatccgac ttacaaaggt aaaaacatga ctgtccgtat cgaacacgat
    22681 ttattgggcg accgcgagat ttccgccgaa gtatattggg gcatccacac cctgcgcgcc
    22741 attgaaaact ttaaaatttc cacacaaaaa atttccgacg taccgcaatt tgtccgcagt
    22801 atggtgatgg tcaaaaaggc gacggcacag gcaaacggcg aattgggtgc agtaaaaccc
    22861 gaaatcgccg ccgccatcga aaaagcctgc gacgaagttt tgttaaacaa ccgctgcctc
    22921 gaccaattcc cgtccgatgt gtatcagggc ggtgcgggta cttcggtcaa tatgaacacc
    22981 aacgaagtca tcgccaacct cgcattggaa gtcttgggat acgaaaaagg ccgctacgac
    23041 atcgtcaatc cgatggatca tgtcaacgcc agccaatcca ccaacgatgc ctaccctacc
    23101 ggcttccgcc ttgccgtgta ttacagcatc ggcgaattgc tcgacaaact ggctgtcctg
    23161 aaaaacgcct ttgccgccaa agccgaagaa tttaaagacg ttttgaaaat gggccgcacc
    23221 cagcttcaag atgccgtacc gatgacggca ggccaggaat tccaatcttt ccaagtgttg
    23281 ttggaagaag aaatcctcaa cctcgaccgt actcgccaac tgcttttgga agtcaacctc
    23341 ggcgccaccg ccatcggtac aggcgtgaac acgcctaaag gctacgccga actggtggtc
    23401 aaaaaactct ccgaagtcag cggcttgcct ttcaaactga ccgaaaacct gattgaggcg
    23461 acctccgact gcggcgcata tgtgatggta cacggcgcat tgaaacgcac ggcggtcaaa
    23521 ctctccaaaa tctgcaacga cttgcgcctg ctctcttccg gcccgcgtgc cggcttgaaa
    23581 gaaatcaacc tgcccgaatt acaggccggt tcttccatta tgcctgccaa agtcaatccc
    23641 gtaattcccg aagtggtcaa ccaagtctgc ttcaaagtca tcggcaacga caccaccatc
    23701 acttttgcag ccgaagcagg tcagttgcag ctgaacgtta tggagccggt catcgcccaa
    23761 tgtatgtttg aaaccatttc cctcttgggc aatgccgcag tcaacctgtc cgacaaatgc
    23821 gtcaaaggca ttacggtcaa ccgcgaaata tgcgaacgtt acgttttcaa ctccatcggt
    23881 ttggtgactt atctgaatcc gtacatcggc caccacaacg gcgatttggt cggcaaaatc
    23941 tgcgcccaaa ccggcaaagg cgtgcgcgag gttgtcttgg aacgcgggct gttgagcgaa
    24001 gaagaactca accgcatcct ctctcctgaa aacctgatga acccccatct gtaacgttgc
    24061 agacaaatgc cgtctgaagc agcatccctg cttcagacgg cattttcggc attcaaggct
    24121 gttttagccg ccgcccgatt ccggcggttt gtcggaaaaa gccggttatt tccgttcggg
    24181 caaaaatcta tcttattaat aaaaaacaat tatttaaaac cattccttaa agcgcgcccg
    24241 cgcggtatgc ggacacggcg ggattatatg ccgaacagtg ccttgacctc cgcccaataa
    24301 aacaggcagg cgatggaaca cagtgccaat acgattcccg ccgtattgat tgtgttgatt
    24361 ttttccttaa aaaacagtgc gcccgaaagc gtacccaaaa caatcacacc gatattcata
    24421 ccggcaaaaa ccaaggtcgg attgtccttc atcatttggt gcgcggtgat gtaggttacg
    24481 atattcataa aattcaaacc gcccaagagt atgccgccga ccacactctc tgcgcgccat
    24541 ctgaccgatt tggcaaacag gcaggcaaac atcagcacac ccgccagcac aaatgcaacc
    24601 agcaggttgc ccgcaaatgc cgttccgctt ttggcaagct gtttgaacag gatatcgata
    24661 atgccgtaac ctgcccacac gcccagcagc aatgccgcct gccgccacgc gcttcctgat
    24721 tttttgccac cgctgtgttt ccaaagcagg cagaacagtg cggcaaaggc gaggcacagc
    24781 ccgattagtt tgccttcgct gagtttttcg ccaaacagcg ttaaagcggc aacaatcggc
    24841 aaaaacagcg acaaacgctg cgccgcgtcg gatttgacga taccggcgga ttctacggat
    24901 ttgcccatta tcacgaatac ggacggcagc agcacgccta atgcggcaaa cagcggccac
    24961 gtcggcaaaa atgcgccgat attgccgaca tccggcttca ataccagcag ggtcagtatg
    25021 accgcgacca cataattgac ggcgaccgcc tgcgcgatgt cgattttttt cttccttgcc
    25081 attttcagca aaatggaaac cgacacgctg aacacgatgc tgattaacag gtaagccata
    25141 ctatatccat ttctcgggtg caaccgccct gccgctgttg aagcggataa agccgacgat
    25201 gatgcggtag aaataccaga cgttcgttgc aataataatc agcacgccga tacccaaaag
    25261 ggcagtcagc gcgcccaaaa gataaagcca aaatgtgcgc caaaaggttt taatcaggta
    25321 ttcggtatgt gtggcataga caatgctgtc caaactgttc cgtttcatat aggcgagaat
    25381 cacgccgact atgggcatat aggcgaaaat cacgccaact atgggcatga acagcatgat
    25441 tgcacccaaa ccgaacagga tgtaggcggc gaggacatag ttgcgcccgc ttccgccgcc
    25501 ggacggttcg taagcatggc gcgaacgtga ggcctgccgt tcaaacgcct gtctgaacgc
    25561 ctgttcgcgg cgcatctgtt cgcggtaaaa ctgctccctg cgcgcctgtt cgcggcggaa
    25621 tgcctcttcc tgccgcccgc gttcctcatg ccgtctgaag gacgcgtcgt attgcatccg
    25681 tttcgacagg tcggaaagcg tatcgtaagc ccgttggatt tctttgaact tttcttccgc
    25741 ctgcggattg cccggattgc ggtcgggatg gtatttcatc gccagcttgc ggtaggcgcg
    25801 tttgatttcg ttcgcacccg cctgcggcga cacgcccaat acggcataca aatctttgtc
    25861 catattgctc cttatacttc ccggtcaaac cgccgcctgt attgttttcg ggttcaaaat
    25921 atttaaatct ccgggacgga tgccgataat gaacccgcgt tttccgccgt tgatatagat
    25981 gatgtccaaa tccatcaccg actgttcgac ataaatatcc aattttgtcc ggatgccgaa
    26041 cggcgttgtg ccgccgacca gatagcccgt ccacttgttt gcctgtgctg gcgtggcggg
    26101 ttcgatgtgt ttcgcaccca aatgccgcgc cagattgcgg gttgaaatct gcttgtcgcc
    26161 gtgcatcaag acaatcagcc ccttaccgtt ttcatcttgc aaaacaatgg ttttaatgac
    26221 caagtgttcg tctttgccga atagtcgggc aaactgcgcc gtgccgccgt gttcctcata
    26281 ggcataggta aaaggttcaa attcgatgcc gttttcacgc aaaacgcgca cggcaggtgt
    26341 tatcggataa gtgtgtttac tcatttttcg tcatgcaaaa catggaaatc ggtcaagcgc
    26401 aatgttacca tatatcccct ttatcgaagg aaaccgatat ggatatccga tatttcggca
    26461 caacgccccg ctattccgaa gccgtcggcg caaacggctt gattttcctc tccggcatgg
    26521 ttcccgaaaa cggtgaaacg gctgccgaac agactgccga cgtgcttgcc caaatcgacc
    26581 gctggctggc ggaatgcggc tcggacaaag cgcacgtttt ggatgcggtc atctatctgc
    26641 gcgatatggg cgactatgcg gaaatgaacg gcgtgtggga cgcttgggtt gccgccggca
    26701 gaacgcccgc ccgcgcctgc gtggaagctc ggctcgcccg tccggagtgg cgcgtcgaaa
    26761 tcaaaataac cgccatgaag aaagatgcgg caaccgcata aacagatttc ctttatattt
    26821 cagagtacgc aaatgatgaa aaccttactg ccctttttcg gcgtggtgcg cgtcagcggc
    26881 gaagaccgcc aaaccttcct gcacggacag ctttccaacg acatcaataa tcttcagacc
    26941 gggcaggcgt gttatgcgac ttacaacacg cccaaaggcc gcgtcatcgc caatatgatt
    27001 gtcgtcaacc gaggcggcga tttgctgctg attatggcgc aagacctgct tgaagcaacc
    27061 gtcaaacgcc tgcggatgtt tgtgttgcgt gccaaagtcg tttttgaaat ccttgaagat
    27121 tacgccgtcg acgcggaatt ggaagcatcc gccgaaccgc ttgccgcgca agaacccaac
    27181 cttgcctttg ccgcacaaca agattcagac ggcatctgct ctatcgctct gcctcatggc
    27241 ggcatccttc gcatcgcacc cgaaaccgcc ctgccccctt atgatgccgc tgccgaaagc
    27301 gcgtggcggc tgcacgaaat ccgcagcggt tatccgtgga tatgcgcggc taccaaagaa
    27361 accgccgtcg cccaaatgct caaccagcac atcatcggcg gcgtacactt caaaaaaggc
    27421 tgctatccgg gacaggaaat cattgcccgt gcccagtatc gggggcaggt caaacgcggc
    27481 ttggcggtat tgtcgggaaa ttcggcagcc gaagcgggca tcctgctgac ggcggacggc
    27541 gaagaggccg gcatcgtact cgacagcgtt aaagattcgg aaaactttac cgcccttgcc
    27601 gtgattaaat tctccgccgc acaaaaaaca ctgtccgccc caaacggcgg cagcttcaaa
    27661 gcggttcatc tcttctttaa aaccgaaaat gcagaataag ccgccgatgc cgtctgaagc
    27721 ccttcagacg gcattgccgc cccgtcaatc ggttacaatg ccgcctttcc aattttcaaa
    27781 cccgaaccgt tatgtctaaa aaaaccaagc aagaactcga aaacaacaaa ctcagcaaac
    27841 gcctgcgcca cgccgtcggc gacgcgatta acgatttcaa catgatcgag ccgggcgaca
    27901 aaatcatggt ctgcctctcc ggcggcaaag acagctatgc cctcttggac atcctgcgtc
    27961 ggctccaagc cagcgcgccg attgatttcc aactggttgc cgtcaatctc gaccaaaagc
    28021 agccgggctt ccccgaagaa gtattgccga cctatctcga aagcatcggc gttccctaca
    28081 aaattgttga agaagacacc tactccaccg tcaaacgcgt attagacgaa ggcaaaacga
    28141 cttgttcgct gtgcagccgc ctgcgccgcg gcatcctcta ccgcactgca aaagaattgg
    28201 gctgcaccaa aatcgccttg ggacaccacc gcgacgacat cctcgccacc ttgtttttaa
    28261 atatgtttta cggcggcaaa ctcaaagcca tgccgcccaa gctggtgagc gacaacggcg
    28321 aacacatcgt catccgtccg ctggcgtatg taaaagaaaa agacttaatc aaatacgccg
    28381 aattaaaaca attccccatc atcccgtgca acctctgcgg ctcgcaaccc aacctgcaac
    28441 gccaagtcat cggcgatatg ctgcgcgatt gggacaaacg ctttcccggc cgcatcgaat
    28501 cgatgttctc cgccctgcaa aacgtcgtcc cttcccacct tgccgatacc gaactcttcg
    28561 actttgccgg attggaacgc ggtcaaaccc tgaaacacgg cggcgatttg gcgtttgaca
    28621 gcgaaaaaat gcccgaacgt ttttcagacg gcagcgaaga agacgaaagc gaaatcaaaa
    28681 tcgcgccgca aaaagccgaa cgcaaagtca tcaacattct ggcgaacaag ccgaaaacct
    28741 gcggcgcgta accccccgcc gtcaaaaatg ccgtctgaag cccgtttggc gtttcagacg
    28801 gaattttaaa agcccgaagc agacggctcc caaacatttg tttctcagta tccgttttca
    28861 gaatgcccga ctgcgggcat attttgcccg atcagttcgg ctatcctctc gccgtcaaac
    28921 tgcgttttga acaccacctt gatttctttg gaaatctcgg caaacagctt ttcggcatgt
    28981 ttgattttcc gctcttcgct ttttcgcaaa tcttccgccc cgtcagtccc tttggcttca
    29041 atcacaaagt tcagaatctc gccgcttttg gttttcacga tataggcaaa atcaggcgaa
    29101 tacgtgccgc cgccggcaac agggattttg atggagtttc tcggtatttt ggtgaatacg
    29161 gttacgcctt caatttggtt gttggcgaca ttttcatgtt ctatatccga atcgtagaaa
    29221 atctcgtcga agagatagcc ggcggcaggc cggtgctccg tatcttcaaa tctgcccaaa
    29281 tctgcttttt ttaccgcgcg cggtttgccg tctttatcgg taaatttggt cggatggatt
    29341 ttgctgccga caagccggta atccagttcg aatttatgga aggaatgatg aagcaaaaac
    29401 cggttgaagc cgtttttgat ttgggcgatg gtctgcatat tcaaaaaatc tccaatgttc
    29461 agttcgtcgc ggacgcggta aaacgcctga tgcaaagtct gcatgcggat ttttgccgtt
    29521 tgtgccagtt tttccagaaa ctctcggtaa ttcattgtgt tgaaacggat aaaatcttca
    29581 tcttcaaaac tgtctatgcg gcgggaaagc ataagcccgt tgttgatata agcttcgttt
    29641 accgccgtgc gtatgcctgc ctgcgggaat ttggcggtgt tttcacgcaa ataggcggta
    29701 aataaatcga caaattcggc ttcatccttg attttgtact gcaaaacggc tttatggtga
    29761 atcagctccc acaaggcttt gagttcttca tatttgcctt cgcgcatgat gatggtgtct
    29821 ttgccctcgt ctttggcgtt gctgactttg cctttgtcca aacctttggg gaaggcttcg
    29881 ggataggcgg cttttaattt gtcatagccg tcttcggcaa agttttcatt gtcgtcaatg
    29941 atgccgtctg aaaacagttg gtttaccaat accagcggtt tgatatcggg gtatttttgc
    30001 aatatttttt gtttcagctc ttcggtaaac tttttggaga tttcttcctg aaaagaattg
    30061 tcgttgattt cgccgacaag ctgcttcaca aagtcttttt cgctgctatc gacaaaataa
    30121 ttcagtttgt acggtacatc gcgcacccgc gccatcagct cgtttaccgg caggcgcagg
    30181 ccgcgtccga cttcttgcag cttggaagtc gtgctgccgc tggaacgcag tttgcaaatc
    30241 tggaaaacgt tgggattgtc ccagccttcg cgcagcgtcc atttggaaaa aataaagcgg
    30301 cgcgggttgt ccaaagacag cagtttttcc ttatcgtgca ggatttcatt gatttcctgc
    30361 tcgattctat cgtcgctgtc tgtattgtct ttggaaaaat agccgccgtg gcaggcggat
    30421 acatcgtcca atgtcttttg caggtaatcg cggtaaaacg ggtcgctttc cgttttcaga
    30481 cggcgtgccg cttccgcgcg aatccagctt tcaaatttat ctttcaggct gcctgaaagc
    30541 tcgttgccgc tgcggtagcc cgcgatatcg tcaataaaaa acagcgtcag cggcttgatt
    30601 ttgggctgtg gcgcgcgttc tgccaaaagc gcgcgttcca gcttgaaatg ttcggcaacc
    30661 gcccgctgca tcatcgcatc ctgcaccgtt tgcgaatagg aataagggtt gatgacggca
    30721 cccgttttca actccaagcc gttgcttaac accaccacgg ttttattcat tttgtcgatt
    30781 ttcaaatccg aaatagccgg atggatttgc gccaaatctt cgccttttgc cagtttgaac
    30841 gtctgctttt tgtccttttc gtttaattca aatttcgctt ctttgccgtc cgacgacacc
    30901 agttttaccg ccgcatccat gccgccctgc atttcttcct gaaacacgcg cacgcctttg
    30961 accagcccgt cgttaaacgc gtctactgcc gtcaaacggt aaagcaagtt gtaatattca
    31021 tcgttaaatg ttgcaccgta gcgcaaaata tattgcggtt ttaagcgttt gatattgccc
    31081 cacgttttcg cgctatctcg ggtcgggaat ttatgcggtt cgtccacaat cataaacggg
    31141 cgcacggcgg ccaatgcatc aacgggattg tcaaacaaat ccttcaatgc cttgtcgccc
    31201 gtatcgttca tggacgacga attaaccatg cccgcgttaa tcagcagcac atgaatttcc
    31261 tttttgtttt ccgctttgac aaattgctca atcgttatgg gcgcattgga ctttttgccc
    31321 ttattctttt tcgcgctttc caccacatag gttttcaggc gtacgccttc ataatcgccg
    31381 ccgaaatcct gttcaaaatg ctctgccaaa gccttgcttt gcaaaaactg ctgtgttccc
    31441 gccttaatgg acaaagtcgg cacgaccacg ataaatttga acacgcccag ccaacggtgc
    31501 agctcgaaca tggtttgtgt gtaggtatag gttttgcccg tgcccgtttc catggaaata
    31561 tcaaggatat tttggtcgtc cgaacggtcg gggaatcggc cgtctatacc gttttggctt
    31621 tgcactttca ggatattgtt tgcgtattgt tttgcagcaa acaaaagttc gggattttcg
    31681 tctgccgtcc gatatttggg cgttgccccg tcaaacacgc ccaaaaccgc cgaaaccgcc
    31741 cgcatttggt gcggctggtt tttctcgtaa ttaaaaccgc tcatgaattg cctccgtcaa
    31801 accctgataa ccacattcaa ctcaatctct tttttattgg cataaccgcg aaccgcctgg
    31861 tcaagttcgt gctgcatggc gcttgccata ttgctgccga atacaatcac gcggttggga
    31921 ttgaaagccg catcgtcgtc cagcttgcgg ataaacgcca acaaatcggc agaagtaaaa
    31981 ccggcattca tcagatacag ccgtttttcg cacagatacg ccgtataact gcccaaccgc
    32041 acaggctcaa ccggcgtggt cagtgccgcc ccgtcataca gcgtccaggt ggtcagaagc
    32101 gtttgcagct gttcttcgct taattcatcg ttaagcggca aatccggttg ttcgggcgaa
    32161 aaatccttgt ccggatgctg cctgaaattg tctgccgttt gaaagatttt gaagcccgaa
    32221 tcgcccgtgt aatcgggatg ttcgacgcgg attttggcgg cggctttttc tatgcgggct
    32281 ttggtgatgt cgaagatggt cgggtagcct gctttacggg cttcggattt ttcagcggtt
    32341 ttttcgggaa gctgtacaca gatatagcgg cggttaccgt tttgtccttc ggcgttaagc
    32401 tgcatcacgg cgtgggcggt tgtgccgctg cctgcgaaga agtctaggat taggtcagtt
    32461 tgatttccct tgaaagttgc agaaagaact aatcttttca ataaatctaa tggttttggt
    32521 gtttcaaaag gagaataacc ttttccagtt tcaaacaact cttctaattc acgaatacca
    32581 tctttagtaa gatttttact tatatttcca atatttaaga tgctatgaaa tttggaaaaa
    32641 tcatcggata cttttctaag ttttccaata attgttttcc cataagactt cggaattaag
    32701 atatattcat tattttcaat atctttatca attttgtttt taaactcaat tccttttatt
    32761 tcaataatat cttcgtaatg caagttccca tatttgccaa actgttcgcg aacggcatct
    32821 tcctgtatcc aatataattc atcattgatt acaatttttt tgccgcgtat atcttttggt
    32881 ctttttagat tgtcaaaatg agtaatatct ttcgcataaa ctaaaacagt ttcatgccca
    32941 tttacaacat attttgcatt accaccacct tctgatgtat taacaattaa atctttaata
    33001 aaattccctt ccccaaacac ttcatcacac aacaacttca actgcgccgc ttcgttatcg
    33061 tcaatcgaga taaaaatcac gccgtcgtcc tttaacagtt cgcgggcgat atacaggcgc
    33121 ggatacataa aggtcaacca tgcactgtgc gagtttgagc ctttgtcggt gaaatctaaa
    33181 atccgcgcgg cttcgtcttc gtcaatattg gctaagcagg caagttcggc tggtgtgaat
    33241 ttgcggtcgt cctgatagac aaagccgtct gatccggtgt tgtagggcgg gtcgatgtaa
    33301 atcatcttca cgctgtttgt gtaggcgttt tttaagtgtt tcaacacttc tagattgtcg
    33361 ccacgaatca gtaggttttg gctgcctgca ttttcgggtt tggcgttgtg cgtcttgtct
    33421 tcacttatca gggtttcggg cggcaggttg cgaagcaggc gagcatatga tttgcccagc
    33481 cagttcattt cgtaaaattc gcgtccgatg tcggtctgtg gcgcaatttc ggcttgtaat
    33541 ctgtcgataa ggaaatttcc gtctgcgtca aaacaggcgg gaaacagttt tttgagctgt
    33601 tcgagttggg tagagttggc ggtaatgccg tctgaagtgt agattgcctc ggtgttcacc
    33661 ccggctgtgt cgattagatc aggggcttg
//
LOCUS       DAWKQB010000005        33002 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.005, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000005 DAWKQB010000000
VERSION     DAWKQB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.005"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            242..861
                     /locus_tag="WJL14_000152"
                     /pseudo
     CDS             242..861
                     /locus_tag="WJL14_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
     gene            884..2335
                     /locus_tag="WJL14_000153"
     CDS             884..2335
                     /locus_tag="WJL14_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ7655284.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            2786..3142
                     /locus_tag="WJL14_000154"
     CDS             2786..3142
                     /locus_tag="WJL14_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ7655285.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            3133..3615
                     /locus_tag="WJL14_000155"
     CDS             3133..3615
                     /locus_tag="WJL14_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ7655286.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            3628..4221
                     /locus_tag="WJL14_000156"
     CDS             3628..4221
                     /locus_tag="WJL14_000156"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ7655287.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            4211..5467
                     /gene="nuoD"
                     /locus_tag="WJL14_000157"
     CDS             4211..5467
                     /gene="nuoD"
                     /locus_tag="WJL14_000157"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ7655288.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELDGEQIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            5467..5940
                     /gene="nuoE"
                     /locus_tag="WJL14_000158"
     CDS             5467..5940
                     /gene="nuoE"
                     /locus_tag="WJL14_000158"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ7655289.1"
                     /translation="MLSAESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     FGISYGETTPDGKFTLVEGECMGACGDAPVMLLNNHKMCSFMTEEAIEKKLAELE"
     gene            6330..7631
                     /gene="nuoF"
                     /locus_tag="WJL14_000159"
     CDS             6330..7631
                     /gene="nuoF"
                     /locus_tag="WJL14_000159"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ7655290.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSGNIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSIGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            7631..8068
                     /locus_tag="WJL14_000160"
     CDS             7631..8068
                     /locus_tag="WJL14_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655291.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            8312..8623
                     /locus_tag="WJL14_000161"
     CDS             8312..8623
                     /locus_tag="WJL14_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7655292.1"
                     /translation="MRNHPLTRQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYQFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
     gene            8905..11166
                     /locus_tag="WJL14_000162"
     CDS             8905..11166
                     /locus_tag="WJL14_000162"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ7655293.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAVQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTVAI
                     LKDALDAESLPSKLNNRAASTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            11169..12245
                     /gene="nuoH"
                     /locus_tag="WJL14_000163"
     CDS             11169..12245
                     /gene="nuoH"
                     /locus_tag="WJL14_000163"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ7655294.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPRLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGLWMISPLNLWK"
     gene            12325..12804
                     /gene="nuoI"
                     /locus_tag="WJL14_000164"
     CDS             12325..12804
                     /gene="nuoI"
                     /locus_tag="WJL14_000164"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ7655295.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            12835..13542
                     /locus_tag="WJL14_000165"
     CDS             12835..13542
                     /locus_tag="WJL14_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655296.1"
                     /translation="MEGLINALKYLAEHEPIDNFEEIRTRNSPIELPSGLSNFEQNIF
                     LKENLSPKLQNDDSLKTHYWIIREWGGIKSFKQSAENSQLIRQFLSELNSGKLSSGLL
                     KISSLSKLASFIDCERFAIYDSRAIFSLNWLLFKFTNADLFFQPQGRNRELEIRNMNV
                     LFHFSDIKPNYRKPDVSFHQYCGLLQDLAKQVYGKQAKPYHIEMLLFKIATTWICADM
                     DQLIKFDCLRNQDFQTA"
     gene            13578..14249
                     /locus_tag="WJL14_000166"
     CDS             13578..14249
                     /locus_tag="WJL14_000166"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ7655297.1"
                     /translation="MTFQLILFYIFAVIILYGAIKTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            14246..14551
                     /gene="nuoK"
                     /locus_tag="WJL14_000167"
     CDS             14246..14551
                     /gene="nuoK"
                     /locus_tag="WJL14_000167"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ7655298.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            14585..15160
                     /locus_tag="WJL14_000168"
     CDS             14585..15160
                     /locus_tag="WJL14_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ7655299.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            15284..15625
                     /locus_tag="WJL14_000169"
     CDS             15284..15625
                     /locus_tag="WJL14_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655300.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            15674..17698
                     /gene="nuoL"
                     /locus_tag="WJL14_000170"
     CDS             15674..17698
                     /gene="nuoL"
                     /locus_tag="WJL14_000170"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ7655301.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAVAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            17754..18074
                     /locus_tag="WJL14_000171"
     CDS             17754..18074
                     /locus_tag="WJL14_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ7655302.1"
                     /translation="MSNTQTIRSADFTTSRAWGALDIANMNGTTVRLHWTDQPYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIVRLKSGDIFYAGIGTEHVAHPRGETRILVIEK
                     EGSV"
     gene            18149..19864
                     /locus_tag="WJL14_000172"
     CDS             18149..19864
                     /locus_tag="WJL14_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ7655303.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            19901..21397
                     /locus_tag="WJL14_000173"
     CDS             19901..21397
                     /locus_tag="WJL14_000173"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ7655304.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLIMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVNGQLNDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNSHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            21407..22852
                     /gene="nuoN"
                     /locus_tag="WJL14_000174"
     CDS             21407..22852
                     /gene="nuoN"
                     /locus_tag="WJL14_000174"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ7655305.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            22964..23263
                     /locus_tag="WJL14_000175"
     CDS             22964..23263
                     /locus_tag="WJL14_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ7655306.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(23372..24268)
                     /locus_tag="WJL14_000176"
     CDS             complement(23372..24268)
                     /locus_tag="WJL14_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ7655307.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVGQAMAAIEMIHAYSLVHDDMPAMDNDSLRRGK
                     PTCHVKYGEATALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSMGMAGGQ
                     AIDLANVGKQMAQTDLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(24252..24476)
                     /locus_tag="WJL14_000177"
     CDS             complement(24252..24476)
                     /locus_tag="WJL14_000177"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ7655308.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(24537..24713)
                     /locus_tag="WJL14_000178"
     CDS             complement(24537..24713)
                     /locus_tag="WJL14_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ7655309.1"
                     /translation="MPSETGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            complement(24740..25663)
                     /gene="rsgA"
                     /locus_tag="WJL14_000179"
     CDS             complement(24740..25663)
                     /gene="rsgA"
                     /locus_tag="WJL14_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ7655310.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(25668..27524)
                     /locus_tag="WJL14_000180"
     CDS             complement(25668..27524)
                     /locus_tag="WJL14_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7655311.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPQALPLNVPQGAIKFEHVDFSYEAGKPLLNGFNLNIKPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVLIDGQDISSVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPDLSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLSEHVEWQHD"
     gene            complement(27550..27687)
                     /locus_tag="WJL14_000181"
     CDS             complement(27550..27687)
                     /locus_tag="WJL14_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655312.1"
                     /translation="MPPEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(27687..28271)
                     /gene="ruvA"
                     /locus_tag="WJL14_000182"
     CDS             complement(27687..28271)
                     /gene="ruvA"
                     /locus_tag="WJL14_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ7655313.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPEGTDVGEGVRLALKNLLK"
     gene            complement(28353..28679)
                     /locus_tag="WJL14_000183"
     CDS             complement(28353..28679)
                     /locus_tag="WJL14_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7655314.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(28733..29452)
                     /locus_tag="WJL14_000184"
     CDS             complement(28733..29452)
                     /locus_tag="WJL14_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ7655315.1"
                     /translation="MTLTRKTLFLLTAAFGIHSFQTASADAVVRAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(29580..30044)
                     /gene="trmL"
                     /locus_tag="WJL14_000185"
     CDS             complement(29580..30044)
                     /gene="trmL"
                     /locus_tag="WJL14_000185"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ7655316.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(30109..30606)
                     /locus_tag="WJL14_000186"
     CDS             complement(30109..30606)
                     /locus_tag="WJL14_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7655317.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            30668..31744
                     /gene="bioH"
                     /locus_tag="WJL14_000187"
     CDS             30668..31744
                     /gene="bioH"
                     /locus_tag="WJL14_000187"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ7655318.1"
                     /translation="MKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIVGA
                     SAMRRQQERKSMPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGDAPF
                     AQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARLTA
                     DEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARCGT
                     PSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRRLKGSRLVVM
                     EKAAHAPFLSHAEAFAALYRDFVEGVLDEPSGRTLAGLPPSCRTCRPTPDTRPQRAQT
                     YPACRGGCGHQPQPAGETLSAGGI"
     gene            32643..>33002
                     /locus_tag="WJL14_000188"
     CDS             32643..>33002
                     /locus_tag="WJL14_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655319.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT         7562 a         8044 c         8918 g         8478 t
ORIGIN      
        1 aatttccatt ttaaaacaat tagtaaaatc gagtttatct tagttgtcca ggacagcccc
       61 attattttta taacaccgtg aagccgcaca gcagtttgaa cagtgatacg ccgtttgcgg
      121 gcttacgagt ttattttccc ggcctgcagt ttgagcaata cggtgatttc ctacggttaa
      181 tacaaatgtt tacacattga tacatttcat ttatagttcc gcctatttga aaatagaaaa
      241 tatgaattcg accgcaagta aaaccctgaa aggattgtcg ctggtgtttt tcgcctctgg
      301 cttctgcgcc ctgatttacc aggtcagctg gcagaggctt ctattcagtc acataggtat
      361 cgatttgagt tcgattactg tcattatttc tgtatttatg gtcggcttgg gtgtaggtgc
      421 gtatttcggt ggacgcattg ctgaccgttt tccttcaagt atcatccccc tgttttgcat
      481 cgctgaagta tccatcggtc tgttcggttt ggtaagcagg agtctgattt ccggcttggg
      541 gcatctttta gttgaggctg atttgcccat catcgctgct gccaatttcc tcttattgct
      601 gcttcctacc tttatgatgg gcgcgacctt gcccttgctg acctgttttt taaccggaaa
      661 atacataatg ttggcgagtc tatcggtacc ttatattttt tcaacacttt gggtgcggca
      721 ctcggatcgc ttgccgccgc cgaatttttc tacgtctttt ttaccctctc ccaaaccatt
      781 gcgctgacag cctgctttaa ccttctgatt gctgcttcag tatggctgcg ttacagaaag
      841 gatggatata gtgaacacta aaccgaatac tagtttgatt tatatgcttt ctttccttag
      901 cggcttattg agcttgggta tagaagtctt gtgggtaagg atgttttcgt tcgcagcaca
      961 gtccgtgcct caggcatttt catttactct tgcctgtttt ctgaccggta tcgccgtcgg
     1021 cgcgtatttt ggcaaacgga tttgccgcag ccgctttgtt gatattccct ttatcgggca
     1081 gtgcttcttg tgggcgggta ttgccgactt tttgattttg ggtgctgcgt ggttgttgac
     1141 gggtttttcc ggcttcgtcc accacgccgg tatcttcatt accctgtctg ccgtcgtcag
     1201 agggttgatt ttcccgctcg tacaccatgt gggtacggat ggcaacaaat ccggacgaca
     1261 ggtttccaat gtttatttcg ccaacgttgc cggcagtgca ttgggtccgg tccttatcgg
     1321 ctttgtgata cttgatttct tgtccaccca acagatttac ctgctcatct gtttgatttc
     1381 tgctgctgtc cctttgtttt gtacactgtt ccaaaaaagt ctccgactga atgcagtgtc
     1441 ggtagcagtt tccctaatgt tcggcatcct catgttccta ctgccggatt ctgtctttca
     1501 aaatattgct gaccgtccgg atcggctgat tgaaaacaaa cacggcattg ttgcggttta
     1561 ccatagagat ggtgataagg ttgtttatgg ggcgaatgta tacgacggcg catacaatac
     1621 cgatgtattc aatagtgtca acggcatcga acgtgcctat ctgctaccct ccctgaagtc
     1681 tggcatacgc cgcattttcg tcgttggatt gagtacaggt tcgtgggcgc gcgtcttgtc
     1741 tgccattccg gaaatgcagt cgatgattgt tgcggaaatc aatccggcat accgtagcct
     1801 tatcgcggac gagccgcaaa tcgccccgct tttgcaggac aaacgtgttg aaattgtatt
     1861 ggatgacggt aggaaatggc tgcgtcgcca tcctgatgaa aaattcgacc tgattttgat
     1921 gaatacgact tggtactggc gtgcctattc caccaacctg ttgagtgcgg aatttttaaa
     1981 acaggtgcaa agccacctta ccccggatgg tattgtaatg tttaatacca cgcacagccc
     2041 gcatgctttt gctaccgccg tacacagtat tccctatgca taccgctatg ggcatatggt
     2101 agtcggctcg gcaaccccgg tagttttccc taataaagaa ctgctcaagc aacgtctctc
     2161 ccggttgatt tggccggaaa gcggcaggca cgtatttgac agcagcaccg tggatgctgc
     2221 agcacaaaag gttgtctctc gtatgctgat tcagatgacg gaaccttcgg ctggtgcgga
     2281 agtcattacc gacgataata tgattgtaga atacaaatac ggcagaggga tttaaccgtc
     2341 ttaaagggtt tcaggcaacg caggttttag gtaacgtcct gctagttcaa aaaaaccgca
     2401 tcacagcagt cgggacaaaa tggtttaaac attttgtccc gaattcttat tcctatatat
     2461 agtggattaa caaaaatcag gacaaagcga cgaagccgca gacagtacaa atagtacgga
     2521 accgatttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact
     2581 ggtttttgtt aatccactat accacgaatt acggtgtaaa aatttatatg accttataaa
     2641 atcaaataag aatcgttatc ataacatgat tgtatttatt gggttttttt gggcgttttg
     2701 ccgatattta ccttttaatg gtttttgaaa ttcgctaaaa tacgaaatta ttgtagaaat
     2761 tttgttaacg gatttgggtg taaccatgtt gtccgcttac tttcccgtct ttgtctttat
     2821 cctcgtcggc ctcgcggccg gcgtgctgtt tatcctgctc ggcacgattt taggcccgaa
     2881 acgccactac gccgaaaaag acgcgcctta cgaatgcggt tttgaagcct ttgaaaacgc
     2941 aaggatgaag ttcgacgtgc gctattacct cgtcgccatc ctcttcatcc tgtttgattt
     3001 ggaggtcgcg ttcatgttgc cgtgggcagt tgtgttcaaa gatttgggcg cgtacggctt
     3061 ctggtctatg ctggtgttta tcgtcgtcct gacggtaggc tttgtttacg aatggaaaaa
     3121 aggtgcgctg gaatgggaat agaaggcgtt ttgaaaaaag gtttcatcac caccagcgcg
     3181 gatacggtgt tgaactatat gcgtaccggt tcgctgtggc cggttacttt cggcttggcc
     3241 tgctgcgccg tggaaatgat gcacgcgggt atggcgcgtt atgaccttga ccgtttcggc
     3301 atcattttcc gtccgtctcc ccgtcaggcc gacctgatga ttgtggcagg tacactgacc
     3361 aacaaaatgg cgcccgccct gcgccgcgta tacgaccagc tcgccgagcc gcgctgggta
     3421 ttgtctatgg gctcgtgtgc caacggcggc ggctattatc actattctta ttccgttgtg
     3481 cgcggtgccg accgcgtcgt gccggtagac gtttatgtgc caggttgtcc gccgactgcg
     3541 gaagccctga tttatggcct gattcagctc caacaaaaaa tcaagcgcac ttccaccatc
     3601 gcgcgtgacg aataaggaga ggacgatatg gcaagcattc aaaacttata tgagaccgtc
     3661 gtcggcgtgc ttggcgatca ggcaagcaaa gtcatttcag ctttgggcga gattaccgtc
     3721 gagtgtctgc ccgaacacta tatttcagtc atgaccgcat tgcgcgatca tgaagagctg
     3781 catttcgagc ttctggtcga tttgtgcggt gtcgattaca gcacttacaa aaacgaagta
     3841 tggcagggca aacgctttgc cgtcgtcagt cagttgcttt ccgttaaaaa caatcaacgc
     3901 atccgcgtac gcgtctgggt ttcagacgac gacttccctg tagtcgaatc cgtagtcgat
     3961 atttacaaca gcgcggattg gtacgaacgc gaagccttcg atatgtacgg catcatgttc
     4021 aacaaccacc cggacttgcg ccgcatcctg accgattacg gctttgtcgg acatccgttc
     4081 cgcaaagact tcccgatttc cggctatgtg gaaatgcgtt acgacgaaga gcaaaaacgc
     4141 gtgatttacc aacctgttac cattgagccg cgcgagatca cgccgcgtat cgtccgtgag
     4201 gagaactacg gtggccaata aattaagaaa ctacaccatc aacttcggcc cgcaacaccc
     4261 tgcggcgcac ggcgtattgc gcatgatctt ggagctggac ggcgaacaaa tcgtccgtgc
     4321 cgacccgcat atcggcctcc tgcaccgagg tactgaaaaa ctggctgaaa ccaaaaccta
     4381 tctgcaagcc ctgccttata tggaccgttt ggactacgtt tccatgatgg tcaacgagca
     4441 ggcgtattgt ttggcggtag aaaagctcgc cggaattgac gtgcctatcc gcgcccagta
     4501 tatccgtgtg atgtttgccg aagtaacgcg catcctcaat cacttgatgg gcatcggttc
     4561 gcatgccttc gacatcggcg cgatgaccgc catcctctac gccttccgcg accgcgaaga
     4621 gctgatggac ttgtacgaag ccgtgtccgg cgcgcgtatg catgcagcct acttccgtcc
     4681 cggcggcgtt taccgcgacc tgcccgactt catgcctaaa tacgagagca gcaaattccg
     4741 caatgccaaa gtattgaagc agctcaacga atcccgcgaa ggcaccatgc tcgactttat
     4801 cgatgccttc tgcgaacgct tccccaaaaa tatcgacaca ctcgaaaccc tcctgaccga
     4861 caaccgcatc tggaaacagc gtaccgtcgg catcggcgtt gtctctcccg agcgtgccat
     4921 gcaaaaaggc tttaccggcg tgatgttgcg cggttcgggc gtggaatggg acgtgcgtaa
     4981 gacacagcct tacgaagtgt acgacaaaat ggatttcgac atccctgtcg gcgtgaacgg
     5041 cgactgctac gaccgctacc tctgccgtat ggaagaaatg cgtcaatccg tacgcatcat
     5101 caaacaatgt tccgagtggt tgcgtgtcaa tccgggtccg gtcattacca caaaccacaa
     5161 attcgctccg cccaaacgta ccgaaatgaa aacaggtatg gaagacctga ttcaccattt
     5221 caaactcttt accgagggta tgcacgttcc cgagggtgag acctacaccg ctgtcgaaca
     5281 tccgaaaggc gagttcggcg tttacatcat ttcagacggc gcaaataaac cctaccgcct
     5341 gaaaatccgc gcacccggct tcgcccacct gcaaggcatg gacgaaatgg caaaaggcca
     5401 catgctggcc gacgtcgttg ccatcattgg tacgcaggac atcgtattcg gggaggttga
     5461 ccgataatgt tatccgcaga atctttaaaa caaatcgaca tcgagttggc aaaatatcct
     5521 gccgaccaac gccgctccgc gattatgggc gcattgcgta tcgcccaaac cgaaaaaggc
     5581 tggctcgctc ccgagaccat cgcctttgtc gcagactata tcggcatctc gcctgcacaa
     5641 gcctacgaag tcgctacctt ctacaatatg tacgaccttg agcctgtcgg caaatacaaa
     5701 ctgaccgttt gtaccaacct gccctgcgcc ctgcgcggcg gtatggcaac cggcgaatac
     5761 ctcaaacaaa aattcggtat cagctacggc gaaaccacgc ctgacggcaa atttaccctt
     5821 gtcgaaggcg aatgcatggg ggcgtgcggc gatgctcctg ttatgttgtt gaataatcat
     5881 aaaatgtgca gctttatgac tgaagaggcg attgagaaga aactggcgga gttggagtag
     5941 gtcgtctgaa acgacgattt aaacgtaggt cggatacttg tatccgacag agtgggtaaa
     6001 aaggcaaaat gtcggattta agaatccgcc ctactgaaat accgaaatgc cgtcattccc
     6061 gcgtaggcgg gaatccaccg gtaagattcg gtttctgaat ttaataagac attgcttacc
     6121 attgaggatg gattcccgcc tgcgcgggaa tgacgacaga caagcaagtg gtcgagatcc
     6181 aacaaaaacg attaaaggtc gtctgaaaat atcgatttga taaactagat tttatttcag
     6241 acgacgttac aagccggtac acaaagacat cttaaggtcg tctgaaacag cggccgcaac
     6301 cgatacgaaa acaaacaggc acaccaaaaa tggctattta ccaatcaggc gtgatttttg
     6361 accaagtgga taccgccaat cccgactgct ggacattgga cgaatacgtc aaacgcggcg
     6421 gctataccgc cctgcgtaaa atcctgtccg gaaacatctc tcaaaccgat gtgattgacg
     6481 aagtcaaaac ctccggcttg cgcggtcgtg gcggtgcggg cttcccgacc ggtttgaaat
     6541 ggagctttat gccccgttcc tttccgggcg aaaaatacgt cgtttgcaat accgacgaag
     6601 gcgagccggg tacatttaaa gaccgcgaca tcatcatgtt caatccccat gccctgatcg
     6661 aaggcatgat tatcgccggt tacgcgatgg gcgcgaaagc cggttacaac tatatccacg
     6721 gcgaaatttt tgaaggctac caacgctttg aggccgcttt ggagcaggcg cgtgccgcag
     6781 gctttttggg taaaaatatt ttgggttcgg attttgaatt tgaactcttc gcccaccacg
     6841 gctacggcgc atatatttgc ggcgaggaaa ccgcattgct cgaatcgctg gaaggcaaaa
     6901 aaggccagcc gcgctttaag ccgccattcc ctgcttcgtt cggcctgtac ggcaaaccga
     6961 ctaccatcaa caatactgaa acgttctcct ccgttccatt cattatccgt gacggtggac
     7021 aggcatttgc cgataaaggt attccgaatg caggcggtac caaattattc tgtatttccg
     7081 gccatgtcga gcgtccgggc aactatgaag tgccgttggg tacgccgttt gccgaagtct
     7141 tgaaaatggc gggcggtatg cgcggcggta aaaaactcaa agccgtcatt cccggcggtt
     7201 cgtccgcgcc cgtattgcct gccgacatca tgatgcagac caatatggac tacgactcga
     7261 tctccaaagc aggctccatg ctcggttccg gcgcgattat cgtgatggat gaagacgtgt
     7321 gcatggtcaa agccctcgaa cgcttaagct acttttacta cgatgaatcc tgcggccaat
     7381 gcaccccgtg ccgcgaaggt acgggctggc tctaccgcat cgtccaccgc atcgtagaag
     7441 gcaaaggccg tatggaagac ttggatttgc tggattctat cggtaaccaa atggcaggcc
     7501 gcaccatctg cgctctcgcc gatgccgccg tcttccccgt ccgcagcttt accaagcatt
     7561 tccgtgatga gtttgtgcat tacatcgaac acggcggacc gatgaaagag cataagtggg
     7621 gagggtggta atggtggaag ctaaaatttt tattctatac ggtgcagcca acaaaggtaa
     7681 gagtacgaca ctcaatacgc tttttaatca gatttgttgg aaattttcta aatttctagt
     7741 cttttttgaa agacatggaa atggcttaga ttttgttgca gtatttgatc atgaaggtca
     7801 gagaattggt ttttattcat ctggtgataa tgaatacgag gttaggagaa atttatacaa
     7861 actttattcg cataattgtg attttatttt tggcacgtca aggacacggg gtggtagttg
     7921 cgatgcagta ggatgttatg cagagttatt gcatggcgat gtaaatataa ttaattggtg
     7981 tgaaaagttt gagcctacag atgaagacaa tgagcgtgct gttaaagagt tatttaagtc
     8041 atttaaaaat ataataaatg agttatagtt ttagttggtt ttatattggt taaaagcaaa
     8101 atgctaaaaa tttaactttg ccgtcattcc cgcgtaggcg ggaatccata gtggaatcta
     8161 aagaaaccga tatttgaaaa gcagttgccg aaattcaaaa aatggattcc cgcctacgcg
     8221 ggaatgacgg cgggagtagg cagatgtttt cagatgaaaa cggttgtaaa tgatattaaa
     8281 aaagttgttg tttatattgc aggaaaaatg aatacgaaac catccgctta ctagacaacc
     8341 tgccgtatat attttggcaa acggtaaaaa tggaacactc tatatcggtg ttaccatgaa
     8401 tttgccggaa agggtttggc agcacaaaaa ccatgtcaat attgatggct ttactgctcg
     8461 atatgatgtg catgatttag tttggtatca gttttttgag aatatgcctg aagcagttgc
     8521 caaagaaaaa acgatgaaaa aatggcgacg tgaatggaag attaaactga ttgaagaaca
     8581 aaatcctgaa tggttggact tgtcgggcgt gttgtttgtt tagttttatt tctggaactt
     8641 taaaaactgt cgttattcca gccccaccta cgcgcagaca ggctacggcg ggaatcaccg
     8701 caaaagttaa gaaaccaatg tttgaaaaca gttactgaaa acccaagaat ggattcccgc
     8761 ctgtgcggga atgacggcaa ggtggcagta aacgttttaa acagtattga ttgtcaatga
     8821 aactcaaaag gccgtctgaa acccattttt cagacgatct ccataaaaaa ttatttatta
     8881 aatacccgta actaggaacg aaccatgtta caaatcgaaa tcgacggcaa acaagtatct
     8941 gtggagcagg gcgcgacggt aattgaagcc gcgcacaagc tcggtactta tattccgcat
     9001 ttctgttacc acaaaaagct ctctattgcc gccaactgcc gtatgtgtct ggtggacgtg
     9061 gaaaaagccc ccaaacccct gcccgcctgt gccacgccgg ttacagacgg catgattgtg
     9121 cgtacgcatt cggcaaaagc ccgagaggcg caagaaggcg tgatggagtt cctgctcatc
     9181 aaccatccgc ttgattgtcc gacctgcgac cagggcggcg aatgccagtt gcaggatttg
     9241 gcggtgggct acggaaaaac caccagccgc tacaccgaag aaaaacgttc cgtcgtcggc
     9301 aaagacatgg ggcctttggt ttccgccgag gaaatgagcc gctgtatcca ctgcacccgc
     9361 tgcgttcgtt tcactgaaga aatcgccggt ttgcaagaaa ttgcgatggt gaatcgcggc
     9421 gaacactccg aaatcatgcc ctttatcggc aaaacggtgg aaaccgagct gtcgggcaac
     9481 gtcatcgatt tgtgtcctgt cggcgcattg accagcaaac cgttccgctt caacgcgcgt
     9541 acttgggaat tgaaccgccg caaatctgtt tccgcccacg atgctttggg cagcaacctg
     9601 attgtacaaa ccaaagacca taccgtccgc cgcgtgttgc cgctggaaaa cgaagcgatt
     9661 aacgaatgct ggctgtccga ccgcgaccgt tttgcctacg aaggcctgta tcacgaaagc
     9721 cgtctgaaaa acccgaaaat caaacagggc ggcgagtgga tggacgtgga ttggaaaacc
     9781 gcattggaat atgtccgcag cgcgattgaa tgtatcgcca aagacggcaa ccaaaaccaa
     9841 gtcggcattt gggcgaaccc gatgaatacg gttgaagagc tgtatctggc caaaaaactt
     9901 gccgacggct tgggtgttaa aaactttgca acccgtttgc gtcaacaaga caaacgtctt
     9961 tcagacggca ttaagggtgc gcaatggttg ggacaaagca ttgaatcttt ggctgacaac
    10021 gatgccgtat tggtagtcgg cgcgaacttg cgcaaagaac agccgctcct gaccgcccgc
    10081 ctgcgccgcg ccgccaaaga ccgtatggca ttgagcgtat tggccggcag caaggaagaa
    10141 ttgtttatgc cgcttctctc tcaggaagcc gcacatcccg acgagtgggc aggccgtctg
    10201 aaaaacttgt ctgccaatgc ggaacacgcc gttaccgcca gcctgaaaaa cgctgaaaaa
    10261 gcagcggtga ttttgggcgc ggaagtgcaa aaccatcctg attacgccgc gatttatgcc
    10321 gccgttcaag agctggcaga tgcgaccggc gcagtgctgg gtattttgcc gcaagccgcc
    10381 aacagcgttg gtgcggatgt cttgggcgtg aactcgggcg agagcgttgc cgaaatggca
    10441 aacgcgccga aacaggcagt cttgctgctc aacgtcgagc ctgaaatcga tacggttgac
    10501 ggtgcaaaag ccgtggctgc gttgaaacaa gcgaaaagcg tgatggcgtt tacgccgttt
    10561 gtcagcgaaa cgctgctgga cgtgtgcgac gtattgttgc cgattgcgcc gtttaccgaa
    10621 acctcgggca gcttcatcaa tatggaaggc cgtctgcaat ctttccacgg cgtggtacaa
    10681 ggcttcggcg attcgcgtcc gatgtggaaa gtgttgcgcg tattgggcaa cctgtttgac
    10741 ctgaaaggtt ttgaatacca cgataccgtc gcgattctga aagacgcgct ggatgcggaa
    10801 agcctgccgt ccaaactgaa caaccgcgcc gcttcgactc aaaaagattt tcagacggca
    10861 tcaagccgcc tcgtgcgcgt gggcggagtg ggcatctatc acaccgatgc tatcgtgcgc
    10921 cgttccgcac cgttgcaaga aaccagccat gccgccgtgc ctgctgcgcg tgtaaatcca
    10981 aatacattgg cacgcttggg cctgcaagac ggacaaaccg ctgtcgccaa acaaaacggc
    11041 gcaagcgtat cggttgccgt caaagccgat gccggtttgc ctgaaaacgt ggtgcatttg
    11101 ccgctgcata ccgaaaatgc cgcgctgggt gcgttgatgg acactattga actggcggga
    11161 gcttgatcat gcaagaatgg ttccaaaacc tctttgccgc aacgctcggt ctgggcgatt
    11221 tgggtattac tgtaggcttg gtggtatccg tcatcgtcaa aattgtgatt atcctgattc
    11281 cgctgattct gaccgtcgcc tacctgactt attttgaacg taaagtcatc ggcttcatgc
    11341 agcttcgcgt cggtccgaac gtaaccggtc cgtggggtct gattcagccg tttgccgacg
    11401 tgttcaaact cttgtttaaa gaagtaaccc gtccgaggct gtcaaacaaa gccctgttct
    11461 acattggtcc gattatgtcg ctcgccccgt cttttgcggc gtgggcggtg attccgttta
    11521 acgaagaatg ggtgctgacc aacatcaata tcggtctttt gtacatcctg atgattacct
    11581 cgctgtcggt ttacggcgtg atcatcgcgg gctgggcttc caactccaaa tattcgtttt
    11641 tgggcgcaat gcgtgcttcc gcacaaagca tttcctacga gattgccatg agtgccgcgc
    11701 tggtgtgtgt cgtgatggta tcgggcagca tgaacttctc cgacatcgtt gccgcacaag
    11761 ccaaaggtat tgccggcggt tcggtattct cttggaactg gctgccgctc ttccccatct
    11821 ttatcgtcta tctgatttcc gccgttgccg aaaccaaccg cgcgccgttt gacgtggcag
    11881 agggtgagtc tgaaatcgtt gccggacacc acgtcgaata ctccggcttc gcattcgcac
    11941 tgttcttcct tgctgaatac attttcatga ttctgatttc cgcgctgaca tcgttgatgt
    12001 tcctcggtgg ttggttgtct ccgttcccac aaagctgggg ctttatcggc acgccttccg
    12061 cattctggat gttcgtgaaa atggcggcgg tgttgtactg gtatctgtgg atccgcgcaa
    12121 ctttcccacg ctaccgttac gaccaaatca tgcgtttggg ctggaaagtg ctgattccga
    12181 tcggcttcgc ctacatcgtg attttgggtc tgtggatgat ttcaccgctg aatttgtgga
    12241 aataagtttc agacgacgta tcaaggtcgt ctgaaaacgc agttttgaat atttaacaga
    12301 atccctgttt tgagggaaca taatatggct aacttagtaa aaacctttct gcttggcgaa
    12361 ttggtaaaag gtatgggcgt aacgctcaaa aacttttttg cccgcaaaga cacgatttat
    12421 tttcccgaag agaaaacgcc gcaatccgtg cgtttccgcg gtctgcacgc gcaacgccgt
    12481 tatccaaacg gcgaggaacg ctgcatcgcg tgtaaattgt gtgaagccgt ttgccccgca
    12541 atggcgatca acatcgaatc ggaagaacgc gaagacggca cgcgccgcac caagcgttac
    12601 gacatcgacc tgaccaagtg catcttctgc ggtttctgcg aagaagcctg cccgaccgat
    12661 gcgattgtgg aaacccatat ttttgaatac cacggcgaga aaaaaggcga cttgcacatg
    12721 accaagccga ttcttttggc cattggcgac aaatacgaag ctgaaatcgc caaacgcaaa
    12781 gccgctgacg cgccgtatcg ttaatgcttt ggggcttctt ggaaggtttt aaatatggaa
    12841 ggactgatta atgcattgaa atatttagcc gaacatgagc caatagataa ttttgaagaa
    12901 attagaacta gaaatagtcc gattgagttg ccaagtggat taagtaattt tgaacaaaat
    12961 atttttttaa aagaaaattt atccccaaaa ttacaaaatg atgatagctt gaagacgcat
    13021 tattggatta tccgtgaatg gggtgggatt aaaagtttta aacaatctgc tgaaaatagc
    13081 cagcttattc gtcaattttt atcggaactt aattcgggaa aattgagtag tggtttgttg
    13141 aaaatttcat cattatctaa attggcttct tttatagatt gtgagcgatt cgccatttat
    13201 gattcacgcg ctattttttc gttgaattgg ttgttgttta aatttacaaa tgcagatttg
    13261 ttttttcagc cacaaggtag aaatagggaa ctagaaatcc gaaatatgaa cgtattgttt
    13321 catttttctg atatcaaacc gaattatcgg aaaccagacg tttcgtttca tcaatattgt
    13381 ggattgttac aagatttggc gaaacaagtt tatggtaaac aagcaaaacc gtatcacata
    13441 gaaatgttgt tattcaaaat tgcgacaacg tggatttgtg cggatatgga tcaactgatt
    13501 aagtttgatt gtttgcgtaa ccaggatttt cagactgctt gaaaccatat ttttgattaa
    13561 taaagaaagc atagactatg actttccaac tgattttatt ttatattttt gcagtgataa
    13621 ttctttatgg cgcgatcaaa accgtcaccg ctaaaaatcc tgttcacgcc gctttgcatc
    13681 tggtgttgac cttctgcgtg agcgcgatgc tttggatgct gatgcaggct gagtttttgg
    13741 gcgtgacgct ggtggtggtt tacgtcggcg ccgtgatggt gttgttcctg ttcgtcgtga
    13801 tgatgttgaa catcgacatt gaagaaatgc gtgccggttt ctggcggcac gcgcctgttg
    13861 ccggtgtggt cggcacattg ttggcagttg ccctgattct gattctggtc aatccgaaaa
    13921 ccgaccttgc cgcatttggg ctgatgaaag acattcccgc cgattacaac aatatccgcg
    13981 atttgggcag ccgtatttat accgattatc tgctgccgtt tgaattggcg gcggtattgc
    14041 tgctgttggg tatggtggcc gcgattgcgc tggttcaccg taaaacggtt aatccgaaac
    14101 gtatggatcc tgccgaccaa gttaaagtac gcgccgacca aggccgtatg cgcttggtga
    14161 aaatggaagc cgtgaaaccg caaaccgaat ctgccgaaga aagcgaagtt tcagacgacc
    14221 tcaagccgaa agaggagggc aaagcatgat taccttgacg cattatctgg tattgggtgc
    14281 gctcctgttc ggtatcagcg cgatgggtat ctttatgaac cgcaaaaacg tgctggtctt
    14341 gctgatgtcc atcgagctga tgcttttggc agtgaacttc aactttatcg ccttttcgca
    14401 acatttgggc gatactgccg gacaaatttt cgtatttttc gtactgaccg ttgctgctgc
    14461 cgaatctgcc atcggtttgg cgattatggt gctggtgtac cgcaaccgac aaacgattaa
    14521 cgttgccgat ttggacgagt tgaaagggta aaggtaggtt gggtcgagac ctgacaagac
    14581 accgatgccg tctgaaaacc cgataggaaa aacgatgaaa tccatagacg aacaaagcct
    14641 gcataatgcc cgccgcctgt ttgaaagcgg cgacatcgac cgtatcgaag tcggtaccac
    14701 cgcgggcctg caacagattc accgttacct gttcggcggc ttatatgatt ttgcgggtca
    14761 aatcagggaa gacaacattt ccaaaggcgg ttttcgtttt gccaacgcca tgtatttaaa
    14821 agaggctttg gttaaaatcg agcagatgcc cgagcggact tttgaagaaa tcatcgccaa
    14881 atatgttgaa atgaacattg cccatccgtt tttggagggt aatggcagaa gtacccgcat
    14941 ctggctggat ttggtgctga agaaaaacct gaaaaaagtc gtgaactggc aaaatgtaag
    15001 caaaaccctg tatttgcagg cgatggaacg cagccccgtc aacgatttag aactgcgctt
    15061 tttgttaaag gacaacctga ccgacgatgt ggacaaccgt gaaatcatct ttaaaggtat
    15121 cgagcagtcg tattattacg aagggtatga aaaaggctga aggtcgtctg aaaagcgatt
    15181 tcagactgtt tcagacgacc tgatttggta ggtgatcaga cgggagcgga tgagaaaaga
    15241 aattctgggt aagaataatc cggtctgaaa tattggaaga agaatgatgg ataaaaatca
    15301 gttagaacaa gaatttcata aagccatgtt aaatatttat caggaggctt tgaatttgcc
    15361 gcaaccttac aaggcgacac gatttttaca aattgtaaat gaatttggtg gtaaagaggc
    15421 ggcggataaa ttattaagta cgggggaaaa gaagactcag accggtttta cagagctgat
    15481 tttgagtggt ggcggagtcc acgccttgaa atacagtatg gaatatctgg tgttacaaaa
    15541 gccgtggtgt gatttattta ctgaagagca attagctgtg gcacgcaaac gattggagcg
    15601 tgttggattt gtttctccga agtaattttg tacgaaacaa acatagattt ttaaatcaat
    15661 cggattcaat caaatgaacg acatgacttt atatctcatc atcgccctcg tgcctttggc
    15721 aggctcgctg attgcgggtt tattcggcaa caaaatcgga cgtgccggcg cgcatacggt
    15781 tacgatactc ggtgtggcgg tgtccgccgt gctgtcggct tatgttctgt ggggattcct
    15841 caatggcagc cgtgccaagt ttgacgagaa cgtctatacc tggctgacga tgggcggttt
    15901 ggatttctcc gtcggcttct tggtcgatac gatgacggcg atgatgatgg tcgtggtaac
    15961 gggcgtatcg ttgatggtgc atatctacac catcggctat atgcacgatg aaaaagtcgg
    16021 ctaccaacgc ttcttcagct atatttcttt gtttactttc agcatgttga tgctgattat
    16081 gagcaacaac ttcatccagc tcttcttcgg ttgggaagct gtgggtttgg tgtcgtatct
    16141 cttgatcggt ttctatttca aacgcccgag cgcgacattt gccaacctga aagccttttt
    16201 gatcaaccgt gtcggcgact tcggcttttt gctcggtatc ggcttggtgc ttgcctattt
    16261 cggcggcagc ctgcgttatc aggacgtatt cgcctatctg cccaacgtgc aaaatgccac
    16321 catccagctt ttccccggtg tggaatggtc gttgattact gtaacctgtt tgctcttgtt
    16381 tgtcggtgca atgggtaaat cggcacaatt cccgctgcac gtctggctgc ccgattcgat
    16441 ggaaggcccg accccgattt ctgcattgat tcacgccgca accatggtta ccgccggttt
    16501 gttcatggta tcgcgtatgt cgccgattta tgagatgagt agcactgcgc tgtctgtcat
    16561 catggtgatt ggtgcgatta ccgccctgtt tatgggtttc ttgggcgtga ttcaaaacga
    16621 catcaaacgt gtggttgcgt attccaccct gtcgcaactg ggctatatga ccgtggctct
    16681 gggcgcgtct gcctattccg tggcgatgtt ccatgtgatg acccacgctt tctttaaagc
    16741 cttgctgttc ttggcggcgg gcagcgcgat tatcggtatg caccacgacc aagatatgcg
    16801 ccacatgggt aacctgaaaa aatacatgcc gattacttgg ctgactatgc tgatcggcaa
    16861 tttgtcgctg atcggtacgc cgttcttctc cggcttctac tccaaagatt cgattatcga
    16921 agcggcgaaa tacagcaccc tgccgggcag cggctttgcc tatttcgccg tcctcgccag
    16981 cgtgtttgtt accgcgtttt acgcgttccg ccaatacttt atggtgttcc acggcgaaga
    17041 gaaatggcgc agcctgcccg aacaccattc agacggccac ggcgaagaac atcacggttt
    17101 gggtaaaaac gacaatccgc acgaaagccc gttggtggtt accctgcctt tgattttgct
    17161 tgccgttccg tccgtcatca tcggctacat cgccatcgaa cccatgcttt acggcgattt
    17221 cttcaaagac gtgatttttg ttaatgccga tgcgcatccg accatgcaca ttatgaaaga
    17281 agagttccac ggcgcattgg cgatggtgtc gcacagcctg cattcgcccg ttctctatct
    17341 cgctatcgca ggcgtattga gcgcatggct cctatacgtc aaactgccgc acctgcctgc
    17401 gaaaatcgcg caggcgttcc gtccggttta cgttttgttt gaaaacaaat actaccttga
    17461 tgccctgtat ttcaacgttt tcgccaaagg cacacgcgca ttgggtacct tcttctggaa
    17521 agtcggcgat accgccatta tcgacaacgg tattgtcaac ggctctgcca gactggtcgg
    17581 cgcggttgcc gcgcaagtgc gtaaagtcca aacaggcttt atctacacct acgctgccgc
    17641 tatggtgttc ggcgtattgg tcttgctcgg tatgaccttc tggggattgt tcagataaaa
    17701 atgagatttt cagacgacgt ttaggtcgtc tgaaaaaaga aacttgagaa acaatgtcaa
    17761 acacacaaac catccgctct gccgacttca ccacttcccg cgcatggggc gcactcgaca
    17821 tcgccaatat gaacggcacc accgtgcgcc tgcactggac ggaccagccc tacaaatggc
    17881 acgtcaacga cggcgaggaa gtgtttgccg tgatggatgg cgaagtcgat atgcactacc
    17941 gagaaaacgg cgaagaacac atcgttcggt tgaaaagcgg cgacatcttc tacgcaggaa
    18001 tcggcacgga acacgtcgcc cacccgcgcg gcgaaacgcg gattttagtg attgagaaag
    18061 aaggcagtgt ttagggcgga tacgcataag tattatgtgc tttttcaggt atcctgaaaa
    18121 tcataaatct atcaacgaga ctttaatcat ggcaaatcta aatgtgagta gtaaaaaaat
    18181 tattaatctg ctaggtaatc aagatatgca ggaaaaatat tttatcattc ctgaatatca
    18241 acgcccctat cgctgggata ccgaaaaatg cgccgtatta tggtctgata ttgtggattt
    18301 tcgccagtcg tccacaggaa aaaatgaaga atattttctt ggtacaatcg ttgtttgccc
    18361 caatgaaaaa ggtaatttgg aagtaattga tgggcaacag cgtttaacat cattactgct
    18421 gttattgcgt gcgttttatc gagttttaga gcaaacggaa agtacccccg ataccgcagc
    18481 caaaattacc acacttaaaa gcaaaattgc cccctgtatt tgggatgttg atgttctaac
    18541 ggctgaaatt accgatagaa ccgcaatccg gctgcatacg caagttgcca cagaaacaga
    18601 taacgaaatt ttgcacgaga ttttggcaaa cggtgaaatc tcagaagagt gtcgttctaa
    18661 ttacgccgtt aattatttgt atttcctgaa acagtgccaa gaatacgcca gaaccaaccc
    18721 aatggattgg tatcatcttt gtttaactat tttgaatgac tgtattttac ttccgattga
    18781 aagtgacagt ttaaatagtg cattaaccat attttccaca ttaaacgata ggggcttgcc
    18841 tttgtcggat gcagatattt ttaaagccag gctttataag atggcgcagc agaaagataa
    18901 atttgttgaa caatggaagg aacttgttgc gttggcagag ggtgcatcta tttccgtaga
    18961 tgatttgttc cgctattaca cccatgtatt aagggcgaaa gaaaagatta cggataaaga
    19021 aattgggctt cgccgttttt atactgacaa ccgaaacaaa cagttggaaa acgaaaattt
    19081 gttcaatcac ttaattgagc tggctggatt ttggcaggat attaacaaca gcaaaactaa
    19141 ccaagacgtg gaaacttcga actttgtaaa tgacgaggcg gccaagtggc tgcaatgtct
    19201 gaatgtctat cctaatgagt tttggaaata cgccgcgagt gtgttcttca ttgcaaacaa
    19261 gggtgcgcaa tcctttaaag atgattttgc agcattatta aaacagctta ctgcattttt
    19321 gtatgctaaa tttttagttg cgccaaccgt aaccgcaatt aaagatgata tttataaatt
    19381 atgtgttgcg atttatcacg gtgaaaaaaa cgctttatca tatgttatgc ctgcacccat
    19441 acactatgaa caaaatacag gacgtataac taaagggttg ttaatgatta atgcttatct
    19501 atttgatgaa aagcagaaat tattgccaac taaaactgaa atcgaacata tctttccgaa
    19561 aaaatggcag gaaactaact ataatggttg gtcaatcata gatgcggaac aatatttaga
    19621 acaactaggt aataaaattg tttttgaaaa acggttaaat attcaggcag gtaacggcta
    19681 ttttggtaag aaaaaggagc aatatcaaaa atccgatatt gttgaagtgc gtgaattagc
    19741 aggtgaaaat gccaatgatt ggttaccgag tgatattcag aaacgccagc agcagattgt
    19801 ggatagattg aaacagtttt tcgataattg ttttgaagca gccagcatcc ccaaaaaaaa
    19861 ttaacttttc caataacctc caaaccaaac aggtaacgca atgttttcta accatcttct
    19921 cagcttggca atatggatac ccattgccgc aggtgtgctg gttttggcga ccggtaaaga
    19981 cagccgtgcg ccgctggcgc gtgtgcttgc cttcatgggt gcgcttgccg gtttcttggt
    20041 aacgctgccc ttgtttaccg gtttcgaccg tttgagcggc ggctatcagt ttaccgagtt
    20101 ccacgagtgg attccgcttc tgaaaatcaa ctacgcattg ggcgtggacg gtatttcagt
    20161 actctttatc atcttgaatg cgtttattac gctgttggtg gtattggccg gttgggaagt
    20221 cattcagaaa cgtccggcgc agtatatggc ggctttcctg attatgtcgg gtttgattaa
    20281 cggcgcgttt gccgcgcagg atgcgattct gttttatgtg ttcttcgagg gtatgctgat
    20341 tccgctgtac ctgattatcg gtgtatgggg cggtccgcgc cgcgtctatg cgtcggtcaa
    20401 gctgttcctc tacacgctga tgggttcgct cctgatgctg gttgccatgg tttaccttta
    20461 ttatcaaaca ggcagcttct ctattgtcga ttttcaaaac atcaaacaga ttccgttggg
    20521 cgtgcaacag cttttgtttg cggcgttctt cctgtcgttt gccgtaaaag tgccgatgtt
    20581 ccccgtacac acttggctgc cggatgccca cgttgaagcg ccgaccggcg gttcgatggt
    20641 gttggcggca attacgctga aactgggtgc gtatggtttc ttgcgcttta tcctgccgat
    20701 tatgccggac gcggctcgct attttgcccc tgtaatcatc gtattgagcc tgattgccgt
    20761 aatttatatc ggtatggtgg ctttggtgca aaccgatatg aaaaaattgg tggcgtactc
    20821 ttccatcagc catatgggtt ttgttacttt gggtatgttc ctgtttgtga acggccaact
    20881 gaatgattgg gcattgaaag gcgcaatcat tcaaatgatt tcccacggtt tcgtgtctgc
    20941 cgcgatgttt atgtgtatcg gcgtgatgta cgaccgcctg catacccgca atattgccga
    21001 ttatggcggc gtggtcaatg tgatgcctaa gtttgcggcg tttatgatgt tgttcggtat
    21061 ggccaatgcc ggtttgccgg cgacttccgg cttcgtgggc gagtttatgg tgattatggg
    21121 cgcggttaaa gtgaatttct gggtaggcgc gttggccgcc atgaccctga tttacggtgc
    21181 gtcttatacc ctgtggatgt acaaacgcgt tatttttggt gcgatccaca attcgcacgt
    21241 tgccgaaatg aaagacatca attgccgcga atttgcgatt ttggcggtgt tggcggttgc
    21301 tgttttgggt atgggcctgt atccgaacgc atttatcgaa gtggtgcatc aggcggcaaa
    21361 cgatttgatt gcccatgtgg cacaaagcaa gatgtgaggt gtgtaaatga actggtctga
    21421 tttgaattta atgcccgcca tgcccgaaat cgtgctgctt gcgctgctgg tgttattgtt
    21481 gctggcggac ttgtgggtca gtgatgacaa acgccgttgg acgcattacg gcgcgttggc
    21541 aacggtggcg gttacggctg tggtgcagtt ggcggtgtgg gaacagggca gcacgtcttc
    21601 gttcaacggg atgtatattg cagacggtat gtcgcgtttg gcaaaaatgg ttttatatgc
    21661 cttgaccttt gccctgtttg tttatgccag accctacaac caagtgcgcg gtattttcaa
    21721 aggcgagttt tacaccctgt cgctgtttgc cctcttgggt atgggcgtga tggtgagcgc
    21781 ggggcatttt ttaactgcct atatcggttt ggaactcttg tcgcttgccc tttacgccct
    21841 gattgccctg cgccgcgatt ccggctttgc cgccgaagcc gccttgaaat attttgttct
    21901 gggtgcgctg gcatccggcc tgctgctcta cggtatttct atggtttacg gcgcaaccgg
    21961 ttcgctggaa tttgccggcg tgcttgcctc ttccttcaat gaagaagcca acgaatggct
    22021 gttgaaactg ggtctggtgt ttatcgtcgt cgccgttgcg ttcaaactcg gcgcggtgcc
    22081 gttccatatg tgggtgcccg acgtgtatca cggcgcgccc acttctgtta ccgccctagt
    22141 cggcactgcc ccgaaaatcg ccgccgtcgt tttcgctttc cgcatcctcg ttaccgggct
    22201 gggaaccgtg catcatgact ggtctctgat gtttgccctg cttgccgccg cctcgctgct
    22261 ggtcggcaac cttgccgcca tcatgcagac caatatcaaa cgtatgctcg cctattccac
    22321 cgtatcgcat atgggtttca tcctgttggc gtttatggcg ggcgcggtcg gctttgcggc
    22381 gggcctctat tacgccatta cctacgcgct gatggcggcg gcagggttcg gcgtgttgat
    22441 ggtgttgtcg gacggggaca acgagtgcga aaacatcagc gatttggcag ggttgaacca
    22501 acaccgcgta tggcttgcct ttttgatgct gctggttatg ttctctatgg cgggtattcc
    22561 gccgctgatg ggcttttacg ccaaattcgg cgtgattatg gcactcttaa aacaaggcta
    22621 tgtttggttg tccgtatttg ccgtcgtgat gtcgctgatt ggcgcgttct actacctgcg
    22681 cgtggtcaaa gtcatgtatt ttgacgaatc cggccgcgcc cgtcccaccg tcggcggcaa
    22741 caatgccgca aaattccttt tgagcgtcaa cgcgctgctg ttggtgctgt ggggcattat
    22801 gccgcaaacc gttatcgact ggtgcgccaa ggcgttggaa aacacgctgt aagccgccgc
    22861 aacggcagcg gcgtcagagg ctgccgtttt tgttaagata tgccgttccg caatgcagtt
    22921 cagacggcat cgccgccgac aatgcccaaa cagaaagccc gccatgaccg catccatgta
    22981 catccttttg ctgcttgcct tgatttttgc caacgccccc ttcctcacga ccaagctgtt
    23041 cggcatcgta ccgctcaagc gcaaacattt cggacaccac ctgatcgagc tggcggcagg
    23101 tttcgcgctg accgccgttc ttgcctacat cctcgaatcc cgtgcgggag cggtacacga
    23161 tcagggttgg gagttttacg ccaccgtcgt ctgcctgtac ctgatttttg cgtttccctg
    23221 tttcgtgtgg cggtattttt ggcacacgcg caacagggaa taggcaggca taggaatgcc
    23281 gtctgaaacc ctttcagacg gcatttgttt cattcaagtg caggccggca tcgctgtgcc
    23341 ggcacgtttc agccggcgat atacgccggt tttaatattt gcgggcgact gcaaattctg
    23401 ccaactgccg caggcgcagg gctttgtcgc cgaagggttc gagcagcgcg accgcttcgg
    23461 caaccagttt gtgtgcgtat gagcgcgccg cttccaagcc catcagtttc acataagtcg
    23521 gcttgtcgtt gtcggcatct ttgcccgccg tcttgcccaa agtcgccgtg tccgcttcac
    23581 aatccaacac atcgtcaatg acttggaacg ccagccccag ttttgccgca taatcgtcca
    23641 ataccgccag actgtcttca gacagattcg ggcaagacat tgcccccaat aaaaccgcgg
    23701 cacggattag cgcacccgtt ttcaggctgt gcatctgttc caaatcggtt tgggccatct
    23761 gcttgccgac attcgccaaa tcgattgcct gaccgcccgc catacccatg cttccgcccg
    23821 cttttgccaa caccgacaac attgccaact ggcgtgcggc gggcagttct gtcggacggc
    23881 tcaatacgtc aaacgcctgc gtctgcaaag catcgccggt cagtagcgcg gtggcttcgc
    23941 catatttcac atggcaggtc ggtttgcccc ggcgcaggct gtcgttgtcc atcgccggca
    24001 tatcgtcgtg aaccagcgaa taggcgtgaa tcatctcgat tgccgccatt gcctgtccta
    24061 ctgcttcatg cacggcttcg cccaattccg aagccgccag aaccagcatc ggccgcaggc
    24121 gtttgccgcc gtccaaagcc gcataacgca tcgcttcgtg cagcgtgtgc gggatttcgt
    24181 tttcagacgg caaaaagcgt tccagcagca gctctgtctg tgcctgcgcc ctctgttgcc
    24241 acgctttcaa atcattcgtc ggattcaagg ttcaactcct tcagcccgtc cgcatccaaa
    24301 acctgtaatt tctgttcgac ttgcgccagc ttggtttggc agtatctgac cagctcgttg
    24361 ccttcctgat aggcggcaag cgcgtcttcc aagggcattt cgccctgcat agcttgtgtc
    24421 agcgattcaa ggcgcgacaa ggcttcttca aacgatttcg gggcgttttt cttcatcgtg
    24481 tttccttttc ggttgaaacc ccgccacttg gacatctgtc cttcggggcg gtagaatcag
    24541 actttatttg ggaggggtgt aagcccttcc aaatcaggac gacacatagg gcggtgcttt
    24601 aagtgtcgtc ctgcgtgttg gaacatagtt tcggatgttc cggtaaaaag cggattgtag
    24661 catttttgaa aaacggatgc cgtctgaaac ccgaatccgg tttcagacgg cattgttttg
    24721 tcctgcaaac ggcaaggcgt tacccgggca gttcgtcggt gatgccctgc aaaaaggcga
    24781 ggcgttcggg gcttgccgcc ctggtttcgg cggcggcttt gaaggcgcag ccgggttcgg
    24841 cgcggtgggt gcagttgtgg aagcggcatt gcccgacaag gtggcggaaa tcggggaaat
    24901 agcgcggcaa atcggcggct tggaggtggt gtaaaccaaa ttcttgcaaa cccggggagt
    24961 cgatgagttg ggtttcgccg ttcaaatcat aaagccgggc gtgggtggtg gtgtgttttc
    25021 ccgagtcgag tgcggcggaa atgtcgccgg tgcgggcggt ttggctgccc aaaagggcgt
    25081 tggtcagggt ggatttgccc ataccgctct gcccgagcag gatgttgctg tgcccttgca
    25141 gggcggggcg caggctgccg gcgttttcca gtgcgcgggt ttcgatgacg ggataaccca
    25201 gcgtttcgta gaatttgagt ttttcgcgcc aaagggcggt ttcgggcagg tcggctttgt
    25261 tcaggacgat gacggcgcgg atgccggcgg cttcggcggc aagcagggcg cgctgtagca
    25321 gccgcacgct cggactcggg acggcggcgg ttacgatgag gagttgggta acgttggcgg
    25381 caatgagttt ggttttccac gcgtcttggc ggtagagcag gctttggcgc ggtaaaaaat
    25441 cttcaatcac aacttgttcg gcgttgacgg ggctgatgcg gacgcggtca ccgcaggcga
    25501 aatcgacgcg ttttttgcgg gtgctggctt cgtaggttgt gccgtcgggc gtgcggacga
    25561 tgtagcggcg gccgtagctg gcggtaattt gggcggtgtc gttcatggtt tctttggggt
    25621 tgggtgtgga aatgccgtct gaaaacgggt gttcggacgg catcggttca gtcgtgctgc
    25681 cactcgacat gctcacttaa gaagccgccg ctctggtgcg cccagagttt ggcgtaaagc
    25741 ccgcgttttt cgagaagttc ggcgtgcgtg ccttcttcga tgatgcggcc tttgtcgagg
    25801 acgacgagcc tgtccattgc ggcgatggtg gagaggcggt gggcgatggc gatgacggtt
    25861 ttgccgtcca tcattttgtc gaggctttct tggatggcgg cttcgacttc ggaatcgagc
    25921 gcgctggtgg cttcgtctaa aagaagaatc ggtgcgtctt tgagcatcac gcgggcgatg
    25981 gcgatgcgct ggcgttgccc gccggagagt ttcacgccgc gttcgccgac gtgcgcgtcg
    26041 tagccgcgcc gccctttggc atcggaaagg tcggggatga agccggcggc ttcggcgcgt
    26101 tcggcggcag aaaccatttc ggcatcggtc gcgtcggggc ggccgtaaat aatgttgtcg
    26161 cgcacggaac ggtgcagcag cgaggtatct tgcgtgacca aaccgatttg ggcgcgtaaa
    26221 gattcttggg tgacgctgct tatgtcctgc ccgtcaatca aaaccgtgcc gctttgcggt
    26281 tcgtagaagc gcaaaagcag gttgacgatg gtggatttgc ccgcgccgct gcgtccgatc
    26341 aagccgactt tttcgccggg tttgatattc aggttaaagc cgttgagcag cggtttgccg
    26401 gcttcgtagg agaaatcgac gtgttcgaat ttgattgcgc cttgcggcac attcagcggc
    26461 agggcttggg gcttgtcgag gatggtgtgc ggtttggaca gggttgccat gccgtcgccg
    26521 acggtgccga tgttttcaaa cagccgcgcg gattcccaca taatgtattg cgacagcccg
    26581 ttgacgcgca acgccatggc ggtggctgta gcaaccgcgc ccacgccgac ctgcccgttg
    26641 tgccagagcc agatgcccag tgcggcggtg gagagggtca gggaggtgtt gacgatgaag
    26701 ctgcacgaat gcagcagcgt cgccagccgc atttgggcgc gcaccgtaac cataaattct
    26761 tccatcgact gcttggcata ggcggcttca cgcgcgccgt gggagaagag tttgacggtg
    26821 gcgatattgg aataggcatc ggtaatgcgg ccggtcatca gcgagcgggc atccgcctgc
    26881 catgcggcgg tttgccccaa tttgggaatc agcaggcgca tcaccgaagc gaaaccgaca
    26941 atccagccga taaagggcag cagcagccat gagtcgagcg aggcgagaat cacgccggag
    27001 gtaatgaaat acaccgacac ataaacgacc atatcggcaa ccgtcatcac cgcgtcgcgc
    27061 aacgccagcg cggtctgcat gactttggcg gacacgcgtc cggcaaattc gtcctgataa
    27121 aaaccgaggc tttggttcag catcaggcgg tggaagttcc agcgcaggcg catggggaac
    27181 acgccctgaa gggtttgcag gcgcacgttg gacgcggcaa acgcccacgc aaccgaaaat
    27241 accatcatcg ccgccattgc cgccagttcc caaccttttt cggcaaacag ttcggcgggc
    27301 gcgtatttgc cgagccactc cacgattttg cccataaatt gaaaaaccag ggcttccata
    27361 atgccgatgc cggcggtcag cgcagccagg gcggctatcc atttccgcac gccggccatg
    27421 ctgctccaga caaaccgcca caagcctttt tctggcgttt tcggggcggc ttcgggataa
    27481 gggtcgattc gggactcgaa ccaggaaaat attttgttca acattgtttt cgatttcggt
    27541 aaaacagttt cagacggcat caaacacaat gccgtctgaa aggaaggaca ataacgccat
    27601 tttacgggaa aagccgtcgg gaagacagcg cgaggcggaa acgcagggtt tcgtcagggc
    27661 aaacgccgcg ccgccttcag gcggcattat ttcagcaggt ttttcaaagc aaggcgcacg
    27721 ccttcgccca cgtccgtccc ctccggaacg cctttgaccg ccgcttttgc ttcgcgttcg
    27781 ctgtaaccca gcgcaagcag cgtgccgacg atgtcttccg tttcgtcggc ggcgggtgcg
    27841 gcggcaaaca gcccgtccgt taccgcatgc gcgaccagct tgccgcgcag ttccaaaacc
    27901 atgcgttcgg cggttttttt gccgattccc ggggcggagg agaggcgttt gacatcttct
    27961 tctgcaaccg cctgcgccag ctcgtctgcc gtcatcgccg acaaaatgcc caaagccgtt
    28021 ttcgcgccga tgccgccgac cttgatcagt tggcggaatg ttttgcgttc ttccgcagtg
    28081 gcaaaaccaa ataaaagatg tgcgtcttcc cgaatgataa gctgggtaaa cagttgtacg
    28141 ctttcaccca cgggcggcag gttgtagaag gtctgcatcg atacgtcggc ctcatagccg
    28201 acaccgttga catcgatgac gatttgcgga gggttttttt caaccagttt gccggtcagt
    28261 ctgctgatca tgtatgccga atcctgaagt gtcgggtgca aaatgccgtc tgaaaccggt
    28321 tcgggcttca gacggcacgg attgtatcaa attcagtcgt cgcggcggga ggaaatcacg
    28381 cggccggtac gggcatcgac aacgactttg tattcctgtc cgtttttgac gatttcgaca
    28441 tcatagtgcg gacggccgtt gtcgtgttcg agatcgatgt cggtgatttt gccgccgaca
    28501 cgcgccaacg ctgctttttc ggcttgggcg cggctgatga ttttgtcttg tttgctgtgt
    28561 tggtgtgcgg cgtgtccgtg gtcgtcatcg ccgtgtccgt cgtggtgggc gagtgcgggg
    28621 gcggaaatgc ccatcagtgc ggttgcggcg gcggtcaaga gaaggcgttt gaagttcatg
    28681 ttttgctcct gcgaagtatc tggtggtgtt tgaaggacgt aaaggcggga catcaaccgg
    28741 cggttaatac cgcccgaacc ataccgcgcg cctgagcttc ggcctcggcg gcgcgttcct
    28801 gcgaggcaaa cggtcccatt ttgacgacgt attcgtaacg gcgtttttca accgagaggt
    28861 tcgggtttga tgccgatgaa gccaggtttt gggcggcttg gttcagatag gcttgtgctt
    28921 cgtgttccgt accgaaagat ttcaagtcga tgaagatgtc tttgttttcg gcaaccggtg
    28981 cggattggcc cgggacgatt tgttcgattt tgacgtgcgc cgtcccttgg ttgacaaagc
    29041 ccaatttttg cgcggcggct ttggatacgt cgatgatgcg gttgccgtgg aaggggccgc
    29101 ggtcgttgac gcggacgatg acgcttttgc cgtttttggt attggttacg cgcacatagc
    29161 tggggatggg cagggttttg tgggcggcgg taaaggcgtt catatcgtat cgttctccgc
    29221 cggaagtttt gcgcccgtga aacctgccgc cgtaccacga ggcgttgccg gtttgcgtga
    29281 attcggcgac ttggtttttc ggcgtgtagc gttttccggc gactttgtag ctgcggttgg
    29341 cggaggcgtg cagtttttct gccctgacca ctgcgtcggc ggatgccgtc tgaaaggaat
    29401 gtatgccgaa tgcggcggtg aggaggaaaa gggtttttcg ggttaaagtc aaaacgtgtt
    29461 ccgttcttga gttgaagacg aatgggcatc atgccctacg gacacgttcc gaaccgctgc
    29521 acgctgcggc agcggttcgg aatgtatccg aacggttttc agaaggcatg aacttgcgtt
    29581 caaacgccgc ctgcgtaacc gtgttgccgc cacgcttcaa agagaatcac ggcaacggta
    29641 ttggaaaggt tcatactccg gctgccgggc tgcatgggca ggcggatttt ttgcgcggcg
    29701 ggcaggctgt cgaggatgtc ggcaggcagt ccgcgcgttt ccggcccgaa cagtaaaacg
    29761 tcgccttttt gaaacgcggt ttcatcgggg cgcgccgtgc ctttggtggt cagggcgaaa
    29821 atgcgcctgc ctgcgagtgc cttgaggcag tcgtcgaagt tttcgtacac cgtcaggctg
    29881 gcgaattcgt ggtagtcgag cccggcgcgt ttcattttgg cggaatccaa tgggaagccg
    29941 agcggtttga caaggtgcaa atccgcgccg gtattggcgc acaggcggat gatgttgccc
    30001 gtgttcggcg ggatttccgg ctggtataaa acgatggtaa acataaatat caatcactta
    30061 taggcgcgta accttgccac aaggcggatg gggtgtcaaa aaatttagtt attttttcat
    30121 tggcgtgcgt gccagcgtcc agcagcagat tcggtttgcg cccgattttt tcagcgtctt
    30181 tgccaattcg tccagcgtcg cgccggtggt aaagacatcg tcgattaaca gaatattaca
    30241 gttttccggt atcggtgtgc ggatttcaaa ggcgtttttg atgtttcgcc gccgttcgcc
    30301 gcctttgagc gtgctttgcg gcggacggtg gtgtcggaat acggtgtgtc ggggcagtat
    30361 ctgccagccg tagcgttgtg ccaacaaccc gacgatgctt tcactttggt tgaagccgcg
    30421 ttgcagcagc cgctccctgc ttagcggtac gggcaggacg aaatcgaaac attcgtctgc
    30481 aagccggtcg ggcggattct gcatcatcag gtctgccagc ggctgcacca tgctcaaatc
    30541 agccaagtgc ttcagcgcgt gtatcatatt gctgacgggc ggttcgtaat gcagcgaagc
    30601 ccacatccgg tcgaatgcgg gcggtttttt ctgacagccg ccgcacaccg cgccgccttg
    30661 aacgtgcatg aaacacaggg ggcagctgtt tgccgcgtcg gtgcggtatg ccgccaaatc
    30721 gtcgcggcag ccggcgcaga tgccgtctga aacgccggac gaaccgtggc ataatacgca
    30781 acgcctgata gtgggcgcgt ctgcgatgcg ccgccaacaa gagagaaaat ccatgcctga
    30841 tgccgtcaaa aaagtttacc tgatacacgg ttggggggcg aaccgccacg tgttcgacga
    30901 tttgatgccg cgcctgcctg caacgtggcc ggtgtccgcc gtcgatttgc ccggacacgg
    30961 cgacgcgccc ttcgcgcaac ccttcgatat tgaggcagcc gccgacgctg ttgccgcaca
    31021 aatcgatacg cccgccgaca ttctcggctg gtcgctcggc ggattggtcg cgctgtatct
    31081 ggcggcgcgc catcccgaca aagtccgttc gctctgcctg acggcgagtt tcgcacggct
    31141 gacggctgac gaagactatc ccgaagggct tgccgcgcct gcattgggca aaatggtcgg
    31201 cgcgttccga acggattatg ccaaacatat caaacagttt ctacaactcc agcttttgca
    31261 cacgcctgat gcggacggaa tcataggcag aatcctgccc gatttggcgc gctgcggcac
    31321 gccctccgcc ttacaggaag ctttggacgc ggcggaaagg gcggatgcgc ggcatttgtt
    31381 ggacaagata gatgttccgg tactgctggt gttcggcggc aaagacgcga ttacgccgcc
    31441 gcgcatgggc gagtatctgc accgccgttt gaagggcagc aggctggttg tgatggaaaa
    31501 ggcggcacac gcgccgtttt tgagccacgc ggaagcgttt gccgcgctgt accgcgactt
    31561 tgttgaaggg gttttagatg aaccatcagg acgcacgctg gcaggtttac cgccatcttg
    31621 ccgaacatgc cgaccaacgc ctgacactcg tccgcaacgc gcccaaacat atcctgcttg
    31681 ccggggcgga tgcggacatc agccgcagcc tgctggcgaa acgctatccg caggcggtat
    31741 ttgaagaata cgattcccgt gcggattttt tggcggcggc ggcagccgcg cgcaaaggcg
    31801 gtttttggca aaggtttgcg ggtaagggcg tggtgcagca ctgccaatcc ccgaccgcgc
    31861 cgctgcccga agcgtgtgcc gatatgttgt ggtcgaatct cggactgttg gcggcggaaa
    31921 gcatcgtccc cgtgctgcaa aattgggcgc gcgcgctgaa gacggacggg ctgctgtttt
    31981 tcacctgctt cgggcgcgat accttggcgg aactgaaatg ccgtctgaaa gaaaacggca
    32041 ttgaaagccg cagtgtgatg tttcccgata tgcacgactt gggcgatatg cttgctgaaa
    32101 acgggtttta cgaccccgtt accgatacgg cgaagctggt gttggattac aaaaaggcgg
    32161 aaacgttttg ggcggatatg gacacgctgg gcatctggcg ggcgatggcg tggaacgatg
    32221 aaaacgccgc gcgttcgtgt gtcgggacaa tatttgagcg ggaaggcggt ttgggcatta
    32281 cgctggaaac ggtgtacgga cacgccgtga aaaaactgat gctgccgcag ggggagaacg
    32341 tggtgcagtt ttttccgaag agatgatgtg cagatgccgt ctgaagctgt ttccaagttt
    32401 cagacggcat ctgtctgtga aaaccgatag aaataaagga aatgccgatg tatgccgatg
    32461 ccggagacgt atattgcgtc tataacttca gactgaagca gtacactgcc tgccaggtta
    32521 cccgagttga agaacacggt gggaaaaaag catatgcaac cctgctggct ttggactggc
    32581 agggcaacaa accgcttggg gcggcggagc tggcggattt gaaaccgctt tacaaagact
    32641 ttatgtattg ggaacgcggt ctgcacatgt ataaggcaag tgccgtcgtg ccgacgggat
    32701 atgtacgggt tgggaatacc gcgccgctgg tcggcgaaga cacgcaacgg tatgcctctt
    32761 tttggggcga cggctacgac gtgtaccgtc agttgagatg gcagcagata cccgaaaaac
    32821 agagaaaggc attcaaaaaa gccgccaaaa gcaaaaagac cgtgatgttt gccggacggg
    32881 aatacggcat atccaaacag aatttgagcg atgtttggga tgattttgaa gacgcgatgg
    32941 aactgaaggc gtttccctgc ctgtcttcgc tgtttctgac caaatggcat aaaaatctat
    33001 at
//
LOCUS       DAWKQB010000006        31929 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.006, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000006 DAWKQB010000000
VERSION     DAWKQB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.006"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            82..1047
                     /locus_tag="WJL14_000189"
     CDS             82..1047
                     /locus_tag="WJL14_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7655320.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQTESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKEQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1211..2179
                     /locus_tag="WJL14_000190"
     CDS             1211..2179
                     /locus_tag="WJL14_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7655321.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2169..3143
                     /locus_tag="WJL14_000191"
     CDS             2169..3143
                     /locus_tag="WJL14_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ7655322.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELVVMMGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSVAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3188..3814
                     /locus_tag="WJL14_000192"
     CDS             3188..3814
                     /locus_tag="WJL14_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655323.1"
                     /translation="MTQERLPEFFDQAPTLTVQDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGTRDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3909..4667
                     /locus_tag="WJL14_000193"
     CDS             3909..4667
                     /locus_tag="WJL14_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7655324.1"
                     /translation="MITIRNVSYRIGTHPILDNISLDIPEGGITALIGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILQKLTREHKCTTVAVLHDINQAAAYADHVVAMKNGKAVLTG
                     TPEEVFTVHNIKELFDMDVDVLDYEGKKLIVHHI"
     gene            4983..6200
                     /locus_tag="WJL14_000194"
     CDS             4983..6200
                     /locus_tag="WJL14_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ7655325.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDATTNALNKLGENITTFAEETKTN
                     IVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVD
                     AKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKDNIAKKANSADVY
                     TREESDSKFVRIDGLNATTEKLDTRLASAEKSIADHDTRLNGLDKTVSDLRKETRQGL
                     AEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTENFAAKAGVAVGTSSG
                     SSAAYHVGVNYEW"
     gene            complement(6266..6604)
                     /locus_tag="WJL14_000195"
     CDS             complement(6266..6604)
                     /locus_tag="WJL14_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ7655326.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            6754..10716
                     /gene="purL"
                     /locus_tag="WJL14_000196"
     CDS             6754..10716
                     /gene="purL"
                     /locus_tag="WJL14_000196"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ7655327.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALDLPEVKLSSEFWYFVG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSGVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNGRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVHLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFTDPNTLTLGVCNGCQMVSNLAEIIPGTAGWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQITQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            10811..11563
                     /gene="gloB"
                     /locus_tag="WJL14_000197"
     CDS             10811..11563
                     /gene="gloB"
                     /locus_tag="WJL14_000197"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ7655328.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLRFAAHIEPDNADIQTALKAAERTPTLPVTLAHERRVNPFLRTEIP
                     AVRQRAEALVGKTLNSGLEVFAALRELKNAYC"
     gene            11797..13251
                     /gene="mgtE"
                     /locus_tag="WJL14_000198"
     CDS             11797..13251
                     /gene="mgtE"
                     /locus_tag="WJL14_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ7655329.1"
                     /translation="MSIEPTPPNLENDGIENDVERVSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEETAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTDMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(13337..14245)
                     /gene="hslO"
                     /locus_tag="WJL14_000199"
     CDS             complement(13337..14245)
                     /gene="hslO"
                     /locus_tag="WJL14_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ7655330.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLVNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            14490..15245
                     /locus_tag="WJL14_000200"
     CDS             14490..15245
                     /locus_tag="WJL14_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7655331.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            15264..15482
                     /locus_tag="WJL14_000201"
     CDS             15264..15482
                     /locus_tag="WJL14_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655332.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            15627..15971
                     /locus_tag="WJL14_000202"
     CDS             15627..15971
                     /locus_tag="WJL14_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ7655333.1"
                     /translation="MNIIRALLIILGCLATGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            15971..16663
                     /locus_tag="WJL14_000203"
     CDS             15971..16663
                     /locus_tag="WJL14_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ7655334.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            16730..17950
                     /gene="argJ"
                     /locus_tag="WJL14_000204"
     CDS             16730..17950
                     /gene="argJ"
                     /locus_tag="WJL14_000204"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ7655335.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            18010..19419
                     /locus_tag="WJL14_000205"
     CDS             18010..19419
                     /locus_tag="WJL14_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ7655336.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLTAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            19608..20999
                     /gene="hrpA"
                     /locus_tag="WJL14_000206"
     CDS             19608..20999
                     /gene="hrpA"
                     /locus_tag="WJL14_000206"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ7655337.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            21100..22227
                     /locus_tag="WJL14_000207"
     CDS             21100..22227
                     /locus_tag="WJL14_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655338.1"
                     /translation="MKHLLIDFENVQPQNLDKLPTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGEIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            22405..23982
                     /gene="lpt3"
                     /locus_tag="WJL14_000208"
     CDS             22405..23982
                     /gene="lpt3"
                     /locus_tag="WJL14_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ7655339.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWITGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGVLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            24084..27173
                     /locus_tag="WJL14_000209"
     CDS             24084..27173
                     /locus_tag="WJL14_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ7655340.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILMIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     DFYTADAVSDGLRPANPQQTAPSYAREERREGKTVAAQTNFSATAANPLPNPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGRGEGKTVASQTNFSATTANPLPNPLPQEGE
                     QSAAASAVSNDPKPKKQPAPPKGRLKPLPLADIRTFQAWLKTAKRDNPRLLFLSRDDL
                     MQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTMTVPLTVLNRLHAPSLEW
                     LVPGMLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAVIIPQLAHFIAKSA
                     GDMRIFEQIDQDAWAAQELPEHCYLNLLIIDDGGQELAGGRKLHELQQQLGQAAAVTF
                     RDNTQEFERDNVTTWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTTEAA
                     EQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAF
                     IGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLL
                     RLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQM
                     WQEKNDGLVKQGLPVSDDLTAFKWMIEELRVSLFAQELKTPYPVSVKRLLKEWEDLN"
     gene            complement(27196..27618)
                     /locus_tag="WJL14_000210"
     CDS             complement(27196..27618)
                     /locus_tag="WJL14_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ7655341.1"
                     /translation="MTDIFTTSKRSFVMLKIHSKETKPEVLVRKFLFFQGFRYRKNDK
                     RYVGKPDIVLSKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAICRERLNRLVREIKDAV"
     gene            complement(27622..29268)
                     /locus_tag="WJL14_000211"
     CDS             complement(27622..29268)
                     /locus_tag="WJL14_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ7655342.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIRRSKGIYQPEFRKESVESNIESGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLNVDFIDNDDEDLIATDRTSLDWKHSKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIEDWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFEDKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(29282..30337)
                     /locus_tag="WJL14_000212"
     CDS             complement(29282..30337)
                     /locus_tag="WJL14_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ7655343.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            30627..30839
                     /locus_tag="WJL14_000213"
     CDS             30627..30839
                     /locus_tag="WJL14_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655344.1"
                     /translation="MPRIHPPHLFPPHIAERGLLYFQQGKVRDVRKTSAGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            31149..31304
                     /locus_tag="WJL14_000214"
     CDS             31149..31304
                     /locus_tag="WJL14_000214"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ7655345.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(31394..31843)
                     /locus_tag="WJL14_000215"
     CDS             complement(31394..31843)
                     /locus_tag="WJL14_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7655346.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINTVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         7923 a         9240 c         7576 g         7190 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacaccc tatgttgcgt ttgaccgcat tgaccctctg caccgtcctc
      121 gccttgggcg cgtgttcgcc gcaaaattcc gactctgccc cacaagccaa agaacaggcg
      181 gtttccgccg cacaaaccga aagcgcgtcc gttaccgtca aaaccgcgcg cggcgatgtt
      241 caaataccgc aaaaccccga acgtatcgcc gtttacgatt tgggtatgct cgacaccttg
      301 agcaaactgg gcgtgaaaac cggtttgtcc gtcgataaaa accgcctgcc gtatttagag
      361 gaatatttca aaacgacaaa acctgccgga actttgttcg agccggatta cgaagcctta
      421 aacgcctaca agccgcagct catcatcatc ggcagccgcg ccgccaaggc gtttgacaaa
      481 ttgaacgaaa tcgcgccgac catcgaaatg accgccgata ccgccaatct gaaagaaagt
      541 gccaaagagc gtatcgacgc gctggcgcaa atcttcggca aacaggcgga agccgacaag
      601 ctgaaggcgg aaatcgacgc gtcttttgaa gccgcgaaaa ctgccgcgca aggcaaaggc
      661 aaaggtttgg tgattttggt caacggcggc aagatgtccg ccttcggccc gtcttcacga
      721 ctgggcggct ggctgcacaa agacatcggc gttcccgctg tcgatgaatc aattaaagaa
      781 ggcagccacg gtcagcctat cagctttgaa tatctgaaag agaaaaatcc cgactggctg
      841 tttgtccttg accgcagcgc ggccatcggc gaagagggtc aggcggcgaa agacgtgttg
      901 aataatccgc tggttgccga aacaaccgct tggaaaaaag aacaggtggt ttacctcgtt
      961 cccgaaactt atttggcagc cggtggcgcg caagagctgc tgaatgcaag caaacaggtt
     1021 gcggatgctt ttaacgcggc aaaataatga aacggcggca ttcgatgccg tctgaaacac
     1081 ggatgcgaac cgcctcctgt gtttcagacg gcattgcccg atacggaggc ttcaaacaag
     1141 gctttccgct ccgacggttc ggactgcctt gtttgaatct tctacgcctt aacgcttttc
     1201 ccttctgttt atgactgcca aacctttttc cctcaacctg accaacctcc tgctgctggc
     1261 ggtattgttt gccgtcagcc tgtcggtggg cgttgccgat ttccgctggt cggatgtgtt
     1321 ttcgctatcc gacagccagc aggtcatgtt catcagccgc ctgccgcgca cgtttgcgat
     1381 tgtgctgacg ggcgcgtcta tggcggtggc gggcatgatt atgcagattc tgatgcgtaa
     1441 ccgttttgtc gagccttcta tggcgggcgc gggtcaaagc gcggctttgg gtttgcttct
     1501 gatgtccctg ctgctgcctg ccgcgccgct gccggtcaaa atgtcggttg ccgccgttgc
     1561 cgcgctgatc gggatgctgg tgtttatgct gctgatccgc cgtctgccgc cgacggcgca
     1621 attaatggtg ccgctggtgg ggattatttt cggcggcgtg gttgaggcag tggcgacgtt
     1681 tatcgcgtat gaaaacgaaa tgctgcaaat gctcggcgtg tggcagcagg gcgatttttc
     1741 gagcgtgctg ctggggcggt acgagctgct ttggattacg ggcggtttgg cggtgtttgc
     1801 ctatctgatt gccgaccggc tgacgatttt ggggctgggc gagacggtca gcgtgaattt
     1861 gggtttgaac cggacggcgg tgttgtggtc ggggctgatt attgtggctt tgattacgtc
     1921 gctggtcatt gtaacggtcg gcaatattcc gtttgtcggg ctggtcgtgc cgaacatcat
     1981 cagccgcctg ataggcgaca ggctgcgcca aagcctgcct gcggtggctt tgttgggcgc
     2041 gtctttggtt ttattgtgcg acattatcgg acgcgtgatt gtgtttccgt ttgaaattcc
     2101 ggtctctacg gtttttggtg tgttgggtac ggctttgttt ttgtggcttt tgttaaggaa
     2161 acctgctcat gccgtctgaa aaaaatatcg gttttatggc aggaagcagc cgtccgttgt
     2221 gggtcgcctt tgcgctgttg ctggtttcct gcatcctgtt tatgacgctc aacgtcaaag
     2281 gcgattggga ctttgtactg cacctgcgtc tgaccaaact tgccgcgctg ctgatggtcg
     2341 cctatgcggt cggcgtgtcc acgcaactct tccaaacgct gaccaacaat ccgattctga
     2401 ccccttcgat tttgggtttc gattcgctgt atgtgttttt gcagaccttg ctggtgttta
     2461 cgttcggcgg cgtgggctat gcttccctgc cgttgacggg caaattcggc tttgaactgg
     2521 tcgtcatgat gggcggctcg ctgctgctgt tctacacgct tatcaaacag ggcgggcgcg
     2581 atttgtcgcg catgatttta atcggcgtga ttttcgggat tttgttccgc agcctttcct
     2641 cgctgctttc gcgcatgatt gaccccgaag aatttaccgc ggcgcaggcg aatatgtttg
     2701 ccggattcaa taccgtccac agcgagcttt tgggcatagg cgcgctgatt ctgctcgtca
     2761 gcgtggcggt cgtttggcgc gaacgctacc gcttggacgt acaccttttg gggcgcgacc
     2821 aagccgtcaa tttgggcatc agctacacgc gcaacacctt gtggatactg ctttggattg
     2881 ccgcattggt ggcgacggcg accgccgtgg tcggcccggt aagctttttc gggcttctcg
     2941 ccgcctcgct tgccaaccac ttttccccgt ccgtgcgcca ttccgtccgc ctgccgatga
     3001 cggtttgtgt cggcggcatc ctcctggtcg gcggacagac cgtattcgaa cacttcttgg
     3061 gcatgaaggc ggtattaagc gtggtggtcg aatttgcggg cggactcgtt ttcctctatc
     3121 tcgttttaaa acacaaaaaa tgacggatgc cgtctgaacg gcagcccgac tcgaaaggac
     3181 aaaccatatg acgcaagaac gtttacccga atttttcgac caagccccga cgctgaccgt
     3241 acaagacccg cttgccgcat ttctcggcgc ggcgcaagac ggcatcctca cttaccgcta
     3301 cgccgatgcc gtgcgcctgt gcggacattc ctgcccgacc gtcgcgggcg cgtacctgat
     3361 ggttatcaaa ggtctgaaag ccctttacgg cgaagagctg cccgaacgcg gcggcatcga
     3421 agccgccatg cagggaacgc gcgacgaagg cacggtcggc gtaaccgcgt ccgtcgtcca
     3481 acttctcacc ggcgcagccc ccgaaaccgg cttcggaggc atcggaatgc agggacgctt
     3541 cgcccgccgc aacctcttat cctttggtgc aggcgaaatc aacggcacac tgaccctgcg
     3601 ccgcaaagac aacggcaaaa ccgtcgccgt cagcctcaac gccgccctgc aacccttcgc
     3661 acccgaaatg cgcgaactta tgcccaaaac cgtcggcggc agcgcaagcg cagaagaact
     3721 cgaacgcttc ggacaactct ggcaggcacg cgttaaagca tttttgaccg aatcggcgga
     3781 cgacccgcag ttcgtcatcg tccgcgaagt gtgagcgttc agacggcatt ccgaatttca
     3841 aatgccgtcc gaacccccgc caaacaacaa caaacctacg cccgacaagc atccgacaag
     3901 catccgccat gattaccatc cgcaacgtca gctaccgcat cggcacgcac cccatcctcg
     3961 acaacatcag cctcgacatc cccgaaggcg gcattaccgc gctaatcggc ccgaacggcg
     4021 caggcaaatc caccctgttt tcctttatgg cgcggctgcg cccgcttgaa agcggcagca
     4081 tcgcctaccg cggcaaaaac cttgccgaca cccccaccgc cgaactcgcc cgcacgctct
     4141 ccatcctcac ccaagaaaac agcatcatga gccgcatcac cgtgcgcgac ctgctgatgt
     4201 tcggccgtta cccctaccat caaggcagac cgactgccga atgccgccgt atcgttaacg
     4261 gtgcaatcga agaattccac ctgcaagacc tctccgaccg ctatctgacc gagctctccg
     4321 gcggccaacg ccaacgcgcc atgattgcga tggtgttctg ccaaagcacc gactacgtcc
     4381 ttttggacga accgctgaac aacctcgata tgtatcacgc ccgctcgctc atgcagattc
     4441 tgcaaaagct cacgcgcgaa cacaaatgca ccaccgtcgc cgtattgcac gacatcaacc
     4501 aggccgccgc ctacgccgac cacgtcgtcg ccatgaaaaa cggcaaagcc gtcctgaccg
     4561 gcacgcccga agaagtgttc accgtacaca acatcaaaga actgttcgac atggacgtgg
     4621 acgtactcga ctacgaaggc aaaaaactga ttgtccacca tatctaaatc cgacaaaaag
     4681 gccgtctgaa cattcagacg gcaacccata tcctgacaaa attaagacac gacaccggca
     4741 gaattgacat cagcataata tgcacatatt aacagatatt aatgccgaac tacctaactg
     4801 caagaattaa ataaataaat aaataaataa ataaataaat aaataaataa ataaattgcg
     4861 acaatgtatt gtatatatgc ctcctttcat atatacttta atatgtaaac aaacttggtg
     4921 gggataaaat acttacaaaa gatttccgcc ccatttttta tccactcaca aaggtaatga
     4981 gcatgaaaca ctttccatcc aaagtactga ccacagccat ccttgccact ttctgtagcg
     5041 gcgcactggc agccacaaac gacgacgatg ttaaaaaagc tgccactgtg gccattgctg
     5101 ctgcctacaa caatggccaa gaaatcaacg gtttcaaagc tggagagacc atctacgaca
     5161 ttgatgaaga cggcacaatt accaaaaaag acgcaactgc agccgatgtt gaagccgacg
     5221 actttaaagg tctgggtctg aaaaaagtcg tgactaacct gaccaaaacc gtcaatgaaa
     5281 acaaacaaaa cgtcgatgcc aaagtaaaag ctgcagaatc tgaaatagaa aagttaacaa
     5341 ccaagttagc agacactgat gccgctttag cagatactga tgccgctctg gatgcaacca
     5401 ccaacgcctt gaataaattg ggagaaaata taacgacatt tgctgaagag actaagacaa
     5461 atatcgtaaa aattgatgaa aaattagaag ccgtggctga taccgtcgac aagcatgccg
     5521 aagcattcaa cgatatcgcc gattcattgg atgaaaccaa cactaaggca gacgaagccg
     5581 tcaaaaccgc caatgaagcc aaacagacgg ccgaagaaac caaacaaaac gtcgatgcca
     5641 aagtaaaagc tgcagaaact gcagcaggca aagccgaagc tgccgctggc acagctaata
     5701 ctgcagccga caaggccgaa gctgtcgctg caaaagttac cgacatcaaa gctgatatcg
     5761 ctacgaacaa agataatatt gctaaaaaag caaacagtgc cgacgtgtac accagagaag
     5821 agtctgacag caaatttgtc agaattgatg gtctgaacgc tactaccgaa aaattggaca
     5881 cacgcttggc ttctgctgaa aaatccattg ccgatcacga tactcgcctg aacggtttgg
     5941 ataaaacagt gtcagacctg cgcaaagaaa cccgccaagg ccttgcagaa caagccgcgc
     6001 tctccggtct gttccaacct tacaacgtgg gtcggttcaa tgtaacggct gcagtcggcg
     6061 gctacaaatc cgaatcggca gtcgccatcg gtaccggctt ccgctttacc gaaaactttg
     6121 ccgccaaagc aggcgtggca gtcggcactt cgtccggttc ttccgcagcc taccatgtcg
     6181 gcgtcaatta cgagtggtaa gcagcatctc ccgataaaga aaccgcagcc ctgcaaggct
     6241 gcggttttta ttttctatcc ggccgtcaga ctgctgcgtc cgcacgttcg cccgtgcgga
     6301 tacggattgc ctcctcgacc ggcagcacaa aaatcttgcc gtcgccgatt tttcccgaac
     6361 gcgccacctc gacaatcacg tcaatcgcgc gttccacagc atcatccgcc aacacaagct
     6421 cgattttgac cttgggcagg aaatcgacag cgtattccgc gccccggtaa atctccgtat
     6481 gccccttctg cctgccgaac cctttgacct cgctgacggt catgcccgta atgccgattt
     6541 ccgtcaacgc ctcgcgcacg tcgtcgagtt tgaacggttt gacaatcgcc tcgatttttt
     6601 tcataaaatt tcctttgaac aaacaataca aacacatccg aaaaacggga acctcccgtc
     6661 agattgtcaa cattttaagc caaaataccc aagcaataca gccccgttgc gcgtataatg
     6721 acagattttc caaccgcatt tgagagccga attatgtctg tcgttttgcc cttgcgcggc
     6781 gttaccgccc tttccgattt ccgtgttgaa aaactcttgc aaaaagccgc cgcactcgat
     6841 ctgcccgaag tcaaattaag cagcgaattt tggtatttcg tcggcagcga gaaagcactt
     6901 gatgccgcga ctgtcgaaaa actgcaagcc ttgttggcgg cgcaaagcgt tgaacaaacg
     6961 cccaaagcgc gcgagggctt gcatttattt ttggtcacgc cccgtttggg tacgatttcg
     7021 ccttgggctt ccaaggcaac caatatcgcg gaaaactgcg gtttggcagg catcgagcgc
     7081 atcgagcgcg gcatggcggt gtggctggaa ggtgcgctta ccgatgaaca gaaacagcaa
     7141 tgggcggctt tgctgcacga ccgcatgact gaatccgtgc tgcccgattt tcagacggca
     7201 tccaaattat tccaccatct cgaatccgaa actttctccg gcgtcgatgt tttgggcggc
     7261 ggtaaagagg ctttggtcaa agccaatacc gaaatgggtt tggcactctc cgccgacgaa
     7321 atcgattatc tggttgaaaa ctatcaggct ttgcagcgca atccgtccga tgttgaattg
     7381 atgatgttcg cgcaggcaaa cagcgaacac tgccgccaca aaatcttcaa cgccgatttc
     7441 atcctcaacg gcgaaaaaca accgaaatcc ctgttcggca tgatacgcga cacgcacaac
     7501 gcgcatcccg aaggtacggt cgtcgcttat aaagacaatt cgtccgtaat cgaaggcgcg
     7561 aaaatcgagc gtttctatcc ggatgcggcg gaaaaccaag gctaccgttt ccacgaggaa
     7621 gacacgcata tcatcatgaa agtggaaacg cacaaccacc cgaccgccat cgcgccgttt
     7681 gcgggcgcgg cgacgggcgc gggcggcgaa atccgcgacg aaggtgcgac gggcaaaggt
     7741 tcgcgtccga aagcgggctt gaccggcttt accgtgtcca acctcaacat ccccggcctc
     7801 aaacagccgt gggaacaaga ttacggcaag ccggaacata tttcctcgcc gctggacatt
     7861 atgattgaag gccccatcgg cggcgcggcg ttcaacaacg aattcggccg ccccaacctc
     7921 ttgggctact tccgcacctt tgaagaaaaa tttgacggtc aggttcgcgg ctaccacaaa
     7981 ccgattatga ttgccggcgg tctgggcagc attcaggcgc agcagacgca taaagacgaa
     8041 atccccgaag gcgcattgct gattcaactg ggcggcccgg gcatgcttat cggcttgggc
     8101 ggcggcgcgg cttcttcgat ggataccggc acgaacgacg cgtctttaga cttcaattcc
     8161 gtacaacgcg gcaaccccga aatcgaacgc cgcgcgcagg aagtcatcga ccgctgctgg
     8221 cagctcggcg gcaaaaaccc gattatctcc atccacgacg tgggcgcggg cggtttgtcc
     8281 aacgccttcc ccgaactcgt caacgatgcc ggacgcggcg cggtattcaa gctgcgcgaa
     8341 gtgccgttgg aagaacacgg tttaacgcct ttgcaaatct ggtgtaacga atcgcaagag
     8401 cgttatgtgt tgtcgatttt ggaaaaagat ttggacacct tccgcgccat ctgcgaacgc
     8461 gaacgctgcc cgtttgccgt ggtcggcacg gcgaccgacg acggccattt gaaagtgcgc
     8521 gacgatttgt tcgccaacaa ccccgtcgat ttgccgctga acgtcttgct cggcaaaccg
     8581 cccaaaacca cgcgtaccga caaaacggtt acgccgtcca aaaaaccgtt tcacgccgac
     8641 gatatcgaca tcaccgaagc cgcctaccgc gttttgcgcc tgcctgccgt agccgccaaa
     8701 aacttcctga ttaccatcgg cgaccgcagc gtcggcggca tgacccaccg cgaccaaatg
     8761 gtcggcaaat accaaacccc cgtagccgac tgcgccgtta ccatgatggg cttcaatacc
     8821 tatcgcggcg aagcaatgtc tatgggcgaa aaaccgaccg ttgccctgtt tgacgcgccc
     8881 gcctcgggca gaatgtgcgt cggcgaagcc atcaccaaca tcgcggcggt caacatcggc
     8941 gacatcggca atatcaaact ctccgccaac tggatggcgg cgtgcggcaa cgaaggcgaa
     9001 gacgaaaaac tctaccgcac cgtcgaagcg gtttccaaag cctgtcaggc attggatttg
     9061 agcattcccg tgggcaaaga cagcctgtcg atgaaaaccg tatggcagga tggcgaagag
     9121 aaaaaatccg tcgtttcgcc gttaagcctg attatctccg cgttcgcgcc ggttcaagac
     9181 gtacgcaaaa ccgttacgcc cgaattgaaa aacgtcgagg acagcgtatt gctgtttgtc
     9241 gatttgggct tcggcaaagc gcgtatgggc ggctctgcat tcggtcaggt atacaacaac
     9301 atgagcggcg acgcgcccga tttggacgac acaggccgtc tgaaagcgtt ttacaacgtg
     9361 attcaacagc ttgtcgccga agacaaactc ttggcatacc acgaccgcag cgacggcggc
     9421 ttgtttgccg ttttggtaga aatggcgttt gcagggcggt gcggcttgga tatagattta
     9481 aatttattgc ttgcacaaac atttattacc aaccataccg ctctgtctca atcattgcgg
     9541 actgaagagg taaaagcgtt ggctgaatgg caagaaacca ttgcccgcac attatttaat
     9601 gaagagttgg gtgctgttat ccaagttaga aaacaagatg ttgccgatat tatcaattta
     9661 ttctatcaac aacagctgca tcataatgtc tttgaaatcg gtacgttaac tgatgagaac
     9721 acgttaatca tccgcgacgg gcaaacgcac cttatttctg acaacctaat caaactgcaa
     9781 caaacctggc aagaaaccag ccatcaaatc caacgcctgc gcgacaaccc tgcctgcgcc
     9841 gacagcgagt ttgccctgat tggcgacaac ggacgcagcg cattgtttgc caacctgaaa
     9901 ttcgacgtga acgaagacat cgccgcgccg tttatcaaca gcggcgcgaa acccaaaatc
     9961 gccatcctgc gcgaacaagg cgtaaacggg caaatcgaaa tggccgccgc cttcacccgt
    10021 gccggtttcg atgcctacga cgtgcatatg tccgacctga tggcaggccg cgtccacctt
    10081 gccgacttca aaatgctggc ggcgtgcggc ggcttcagct acggcgacgt actcggcgcg
    10141 ggcgaaggct gggcgaaatc catcctgttc caccccgcct tacgcgacca gttcgccgcc
    10201 ttcttcaccg acccgaacac gctgacattg ggcgtgtgca acggctgcca gatggtcagc
    10261 aacctcgccg aaatcatccc cggcacggca ggctggccga agttcaagcg caacctgagc
    10321 gaacagttcg aagcgcgcct gagcatggtt cacgtcccca aatcagcctc cctgattctg
    10381 aacgaaatgc aaggctccag cctgcccgtc gtcgtcagcc acggcgaagg ccgcgccgac
    10441 ttcgcgcttc acggcggcaa catttctgcc gatttgggca ttgcgttgca atatgtggac
    10501 ggacaaaacc aaattaccca aacctacccg ctcaacccca acggctcgcc gcaaggcatc
    10561 gccggcgtga ccaacgccga cggccgcgtt accatcatga tgccgcatcc tgaacgtgta
    10621 taccgcgccg cacaaatgag ctggaaaccg gaagactgga cggaattgtc cggctggtac
    10681 cgcctcttcg ccggcgcacg taaagccttg ggctaaccgc cctactcaaa ccaatgccgc
    10741 ctgaagaata tttcagacgg cgttccggca taccatcctt taaacggtat ccgtccaccg
    10801 aggaacactc atgaaaatca cacccgtcaa agccttgacc gacaactaca tctggatgat
    10861 acagcacggc aaccatgccg tctgcgtcga cccttccgaa ccctcgcccg tcttggaatt
    10921 cctcgtccgc aaccgcctca tgcttgccca aacatgggta acgcaccccc accccgacca
    10981 cgagggcggt gcggcggcac tctggcgcgg ctacatggaa tcgcccgttt acggcgaatc
    11041 cgacatcgaa gcagcaaccc acaccgtaac cgccggcacc caattcacct tcggcgacgg
    11101 acaggttacc gtttgggcaa cacccggcca cacagaccgc cacaccagct accttctcga
    11161 aacttcagac ggcatacacg tcttttgcgg cgacaccctt ttttccgccg gctgcggacg
    11221 cgtgtttacc ggcacaatcg aacagcttta cgacagcttc caacgcttca accgcctgcc
    11281 tgaaaacacc ctgttctacc cggcacacga atacaccgcc gccaacctgc gtttcgccgc
    11341 ccatatcgag ccggacaacg ccgacattca gacggcattg aaagcggcag aacgcacgcc
    11401 caccctgccc gttaccctcg cccacgaacg ccgcgtcaac ccgttcttac ggacagaaat
    11461 ccccgccgtc cgccaacgtg ccgaagccct ggtcggcaaa acgctgaaca gcggtttgga
    11521 agtattcgcc gccctgcgcg aactgaaaaa cgcctactgc tgacctgccc tccgaaaaat
    11581 gccgtctgaa acccgcgttt cagacggcat ttgcgttaaa aatagtaaac cgtttcaaaa
    11641 gggagtagaa tagtgccgtt tccaaccctg cgcctgtacc gtcaggcttt tattatggac
    11701 cttcccagtt cgtttttact gaacacccct tccgattcca aacgacaata accatcccgc
    11761 cggaatgcct ccccgcgcac ggcgggcgga gcatttatga gcatcgaacc aacccctccg
    11821 aaccttgaaa acgacggtat cgaaaacgat gtagaacgcg tttccgccga tttcgaccgt
    11881 gtccactccc tctgcgaaat cctcgaacct gcttttgaac aaatcgaaaa cggtacaccg
    11941 ctcgaagacg cgccgctgcg cgacaagctg accgagctga ccgtcctctt gagcgagctg
    12001 caccctgccg acgtggcggc ggtgttggaa tcgctgccgc cgcgcgaacg caatatcgtc
    12061 tggattttag tgaaacccga agacgacggc gaagtattgc tggaagtatc cgacgcggtg
    12121 cgcgaaacgc tgatcgagtc gatggacaaa gacgaattgt tggcggcggt cgatgatttg
    12181 gacgcggacg aattggcgga gctggcagac gatttgccgc accaagtggt ttatgaagcc
    12241 ttacagacgc gcgatgagga agaacgcgcc caagtcaagg cggcaatgtc ctacgaagac
    12301 aaccaagtcg gtgcgattat ggacttcgag ttggtcagca tccgcgccga tgtcgcctgc
    12361 gaagtggtgc tgcgttacct gcgccgcttc gacagcctgc ccgaccatac cgacaagatt
    12421 tttgtggtcg atgaaaacga tgtgttgcag ggcgtgctgc ccatccgcaa acttttggtc
    12481 gccgatcccg aagatttggt ggaaaacgtg atggcgaaag atgtcgtgcg tttccgcgcc
    12541 gaagacgacg tggaagaaac ggcgcaggca ttcgaacgct atgacttggt taccgcgccc
    12601 gtcgtcgatg aaaacaaaaa gctcatcggc agggtgaccg ttgacgaaat ggtggacgtg
    12661 atccgcgaag aatccgaagc ggatatgttg aatatggcgg gtttgcaaga agaggaagac
    12721 ttattcgccc ccgtcttgga ttccgtcaaa aaccgctgga tgtggctcgc cgtcaacctc
    12781 tgcaccgcct tcctcgccag ccgtgttatc ggcgcgtttg aaggcagcat cgaaaaaatc
    12841 gtcgcactcg ccgcgctgat gcccatcgtc gccggcatag gcggtaactc gggcaaccag
    12901 acgattacca tgattgtccg cgcgatggcg atggggcagc tgacggatat gcaggcgggg
    12961 cgtttgctga aaaaagaagt cggtgtcgcc ttggtcaacg gcatcatttg gggaacggtc
    13021 atgggcgcag tatcttggct gctttacggc agcctcggca tcgggctggt tatgattgcc
    13081 gcgatgacgc tcaacctcct gctggcggca accgtcggcg tattaattcc cgtggtaatg
    13141 gaaaagttcg gacgcgatcc cgcactgggc agctcggtgc tgattaccgc cgttaccgac
    13201 tccggcggct tcctgatttt cttggggctc gccaccctat tcctgcttta aatgccgtct
    13261 gaacccgcgc aaaaatgccg tctgaagcgg aagctgcttc agacggcatt tgactattta
    13321 tccttgttgc acaagattat tggacggtat gccggggcag ccctttggca acgccgacca
    13381 catcctcccc gaacagcgcg ttgacatcgg tttcgtcaaa cacatatttg ctgtggcaga
    13441 aatcgcaatc gacttcgatg ctgccttgtt ccaccaccac gccgccgact tcttccccgc
    13501 ccagcatcaa cagcatatcg ctgactttgc cgcgcgaaca ggtgcatgaa aattcaaacg
    13561 tttccggctc gaacacgcgc ggcggcgttt cgtggaacag gcggtataaa acgtgttgcg
    13621 cgtccagtcc tgccagctcc tccgccgtca gcgtgcgcgc cagcgtactg acgtgttccc
    13681 atgcctcttc atccaatacc tcttcaggca gacgctgcac cagcagaccg cccgccgctt
    13741 cgtcgcttgc agacaggacg atgtgcgtat caagctgttc ggaacgtttc atatagttca
    13801 ccaacatttg cgcgataccg tcgccttcca aaggcactac accctgccag ggttcgccgt
    13861 ctttaggctg cagcgtcagc acgaatacgc cgccctcgcc caaaagatcg ccgaggcttt
    13921 cgtcatcggc tatttctgcg gtttcgtccc accgcgcggt cgcacggacg gtacggtcgg
    13981 aagtcgcttc cgcaaccagc attttcagcc gcccctgccc ctgaacctgc acaatcagcg
    14041 tgccttcgtt tttgaggttg cccgacagca gaacaccggc agccaacagc tcgcccaagg
    14101 cgcggcggat ggcggcggga tagtttttct gttttacaat gtgctgccac acgttttcca
    14161 gacggacgtg cagcccgcgc acgggcatat cgtcgaagat aaagcgggta cgcacatcgg
    14221 cgcggttgat ggcggtttga ttcatgattt tctctgactg attgttcgga tggcggctat
    14281 atggttgcgg tcggcgcgaa aacaagacgg acggcggatg cacttcccaa attatcaata
    14341 aattatataa aaatcaacat attaactcaa tctaacaagc cgttttttgc caaacagccg
    14401 tttttttata tacaatcaac aagatatttt cgactgatac agcataacat cgcacggcgg
    14461 cacgatgcct cctgcgcgga aacaccgata tggattcttt tttcaaaccg gcagtttggg
    14521 cggttttgtg gctgatgttt gccgtccgcc ccgcccttgc cgacgagttg accaacctgc
    14581 tcagcagccg cgagcagatt ctcagacagt ttgccgaaga cgaacagccc gttttaccca
    14641 tcaaccgagc ccccgcccgg cgggcgggca atgccgacga actcatcggc agcgcgatgg
    14701 ggcttaacga acagcccgtt ttacccgtca accgagtccc cgcccggcgg gcgggcaatg
    14761 ccgacgaact catcggcaac gcgatggggc ttaacgaaca gcccgtttta cccgtcaacc
    14821 gagcccccgc ccggcgggcg ggcaatgccg acgaactcat cggcaacgcg atgggacttt
    14881 tgggtattgc ctaccgctac ggcggcacat cgatttctac cggttttgac tgcagcggct
    14941 tcatgcagca catcttcaaa cgcgccatgg gcatcaacct gccgcgcacg tcggcagaac
    15001 aggcgcggat gggtacgccg gttgcccgaa gcgaattgca gcccggggat atggtgtttt
    15061 tccgcacgct cggcggcagc cgcatttccc atgtcggact ttatatcggc aacaaccgct
    15121 tcatccacgc gccgcgcacg gggaaaaata tcgaaatcac cagcctgagc cacaaatatt
    15181 ggagcggcaa atacgcgttc gcccgccggg tcaagaaaaa cgacccgtcc cgctttctga
    15241 actgatttcc caaggaatac gcaatgagta tgcccgaaat gcccaaatgg tacgacgatg
    15301 acggacagat cgtgtcctgt accgaaaagg tcaaagtgat gtccgaaaat atggccgagc
    15361 tgtatcagac ggcacaagac gcgtttgaag acgcgctgct gatgggttgc ggcgaacgtc
    15421 agttgcgcga ttacctgctc gcgctgattg aaggtttgga aaatccctac cgcaaagtct
    15481 gaacacgccc cggttgctgc ggcacggttt atccgtgccg tttttgcgtt tgtgcgcggc
    15541 ttcggctttt cagacggcat atttgacgtt atgattaaac agttaacaag atttatcaca
    15601 acgccgtcaa aagacagaca cacaacatga acatcatccg cgcgctcctc atcatcctcg
    15661 gctgcctcgc caccggcgaa accgccgttt tcctagcagg catcaaactg cccggcagca
    15721 tcgtcggcat gggcgtactg tttgcgcttt tgcaggcggg ttgggtcaaa acgtcttggc
    15781 tgcaacagct taccgacgcg ctgatggcga atctgacgtt gtttctcgtg ccgccctgcg
    15841 tggcggtcat cagctatttg gatttgattg ccgacgattg gttttcgata ctggtttccg
    15901 cctccgccag cactttgtgc gtactgctgg ttacaggcaa ggttcaccgc tggatacgga
    15961 gcattatctg atgaacgaaa tcctcaggca gcccagcgtt ctgcttttcc tcacgcttgc
    16021 cgtgtacgcg cttgcgatta tcgtgcgcac gcgcacgggc aatatcttct gcaaccccgt
    16081 actcgtcagc actatcgtgc tgattgccta cctgaaaatc ctcgacatcg attatgcggt
    16141 gtaccacaac gccgcgcagt ttatcgattt ctggctcaag cccgccgtcg tcgtgcttgc
    16201 cgtgccgctc taccaaaacc gccgtaaaat cttcaaccaa tggctgcccg tcatcgtttc
    16261 acagcttgcg ggcagcgtta cgggcattgt tacggggatg tattttgcca aatggctggg
    16321 cgcggaacgc gaagtcgtcc tctcgctcgc gtccaaatct gttaccaacc ccatcgccat
    16381 tgaaattacc cgctccatcg gcggcattcc cgccattacc gccgccaccg tcatcattgc
    16441 cggtctggtc ggacagattg ccggttacaa aatgctgaag aacacagtcg tcatgccctc
    16501 gtccgtgggt atgtcgctcg gcacggcttc gcacgcgatg ggcattgccg cctcgctcga
    16561 acgcagccgc cgcatggcgg catacgcggg gctggggctg acgttcaacg gcgtactgac
    16621 cgcgctgatt gcgccgctgc ttatccccgt tttggggttc tgaacccgtt tcagacggca
    16681 tttcagccca tgctgtctga acgccgacac actcgcaagg agaaccgtta tggctgtcaa
    16741 cctgactgaa aaaaccgccg aacaactgcc cgacatcgac ggcattgccc tctacaccgc
    16801 ccaagcaggc gtgaagaagc ccgggcatac cgacctgaca ctgattgccg tagccgccgg
    16861 cagcaccgtc ggtgcagtct tcacgaccaa ccgtttctgt gccgcgcccg tccacatcgc
    16921 caaatcgcac cttttcgacg aagacggcgt gcgcgccctc atcatcaaca cgggcaacgc
    16981 caacgcgggt acgggcgcac agggcagaat cgatgctttg gcagtgtgtg ccgccgccgc
    17041 ccggcaaatc ggctgcaaac cgaaccaagt gctgcccttc tccaccggcg tgattctcga
    17101 accgctgccc gcagacaaaa tcatcgccgc gctgcccaaa atgcagcctg ccttctggaa
    17161 cgaagcggca cgcgccatca tgaccaccga caccgtgccc aaagccgcct cgcgcgaagg
    17221 caaggtcggc gacaaacaca ccgtccgcgc cacgggcatc gccaaaggct cgggcatgat
    17281 tcatcccaat atggcgacca tgctcagttt catcgccacc gatgccaaag tttcccaacc
    17341 cgtcctccaa ctgatgacgc aggaaatcgc cgacgaaacc ttcaacacca tcaccgttga
    17401 cggcgacacc agcaccaacg acagcttcgt catcatcgcc accggcaaaa acagccaaag
    17461 cgaaatcgac aacatcgccg acccgcgtta cgcccaactc aaagaattgt tgtgcagcct
    17521 cgcgctcgaa ctcgcccaag ccatcgtccg cgacggcgaa ggtgcgacca agttcatcac
    17581 cgtccgcgtc gaaaacgcca aaacccgcga cgaagcccgc caagccgcct acgccgtggc
    17641 acgttcgccg ctggtcaaaa ccgccttttt cgcctccgac cccaacctcg gcaggctgct
    17701 cgccgccatc ggttatgccg gcgttgccga cctcgatacc gacctcgtgg aaatgtatct
    17761 cgacgatatt ttggttgccg aacacggtgg acgcgccgca agctacaccg aagcacaagg
    17821 gcaggcggtg atgtcgaagg ccgaaatcac cgtccgcatc aagctgcatc gcggacaagc
    17881 cgccgccacc gtctatacct gcgacctgtc gcacggatac gtttccatca acgccgacta
    17941 ccgttcctga cccgacacgg cttcagacgg catacataaa atgccgtctg aaccgccgga
    18001 caacatacca tgacctccac attcccccgc cgcctcgccc gcaaaatccg ccaaacccgc
    18061 cgcctgtcgc gcaaaagcat cgcctttctg ttccttttgg caggttcggc actcgtcgcc
    18121 ctgaccgcgc tgttttttgc ccatcttgcc gattttgcgc tggaactgaa cgccaaactg
    18181 gttcaacaat acccgtggtt cgcgtgggtc gcgcttcctt tgggtttacc gcttattgcg
    18241 tggctcacac gcaaattcgc ccccttcacc gccggcagcg gcatcccgca ggtcatcgcc
    18301 tcactgtcgc tgccctacgg cgcacagaaa acgcggctga tccgcctcgg gcagacgctg
    18361 ctgaagattc cgctaacctt tttgggtatg ctgttcggcg cgtccatcgg acgcgaaggt
    18421 ccgtccgtgc aggtcggcgc ggcagtgatg ggcgcgtggg gcgcgtggtg caagaaacac
    18481 ggcttggcat tcaaagggat gcaggaaaac gatttgatgg cggcgggcgc ggcgggcggt
    18541 ttggcagccg cgttcaacgc gccgctggcg ggcgtgattt tcgccattga ggaactcggg
    18601 cgcggcatca tgttgcgctg ggaacggcag attctgctcg gcgtactcgc cgccggtttc
    18661 atacaggtcg ccattcaggg caacaacccg tatttttccg gcttcaacgg cggcgtattg
    18721 gaacatatct ttctgtgggt cgcactgtcc ggcctggttt gcggcgcggc gggcgggctg
    18781 ttcggacgtt tgctctatcg cggtgcggcg gcgtttgcac cgcgcaagat acgcggcttc
    18841 atccgcaacc gtccgctgct gctggcggca ctgatggggc tgctgctcgc cctgctcggc
    18901 acgttctacc aaggcaaaac ctacggcacc ggctaccacg aagccgccca agccctgcac
    18961 ggcatctacg aagccccctt cggactcacc gccgccaaat ggctcgccac cgtattcagc
    19021 tattgggcag gcattccggg cggcattttc actccctcgc tgaccatagg cgcggttttg
    19081 ggcgagcata tcgccgccat cgccgacata tcgcagggtg caaacatcat cgtcctcatc
    19141 tgcatggcgg catttctggc gggcgcgaca caatccccga ttacttccgc cgtcgtcgtc
    19201 atggaaatga cgggcggaca aagcctgctg ttttggatgc tgattgcctg cattttcgcc
    19261 tcgcaggttt cgcgccagtt ttcgccgcgt ccgttctacc acgcatcggg aatgcgcttc
    19321 cgccagcgcg tgcttcaaga aaccgccgcc caaaccggca atgcgcccgc aagaccgcaa
    19381 acagcaaaca gcaaaacggg aatgccgtct gaaaattaaa acgcccccga tcaaacgccg
    19441 gcagccgcct tgattcgaat accgttccgc cgccgcttga aatttcagca acaatgccgt
    19501 ctgaacgaca gaatgcggtt ttcagacggc atttccccat cccgatattg cctaaacaaa
    19561 accgaagcgt ttgctataat tgcacttttt accgcacacg cacgcccatg tttcccgatt
    19621 tctcccaaac cctctccaaa gaccgccact tcctgcaatc cgccttcaaa aatcccaaca
    19681 aatacggcgg tttgtccaaa gtcgaagaaa aataccgaaa atcgcacgaa atctttttga
    19741 ggcgtttggc cgcattgccc aaacccgaat tcgacaacac cctgcccgtt cacgaaaaac
    19801 tcgaagaaat caaaaaaacc gttgccgaac atcaggtaac gattatttgc ggcgaaaccg
    19861 gttcgggcaa gaccactcag ttgcccaaga tttgtttgga actcgggcgt ggggcggcgg
    19921 ggctgatcgg gcatacccag ccgcgccgtt tggccgcgcg ctccgtggca gagcggattg
    19981 ccgaagagct gaaatccgaa atcggcagcg cggtcggcta taaagtacgc ttcaccgacc
    20041 acacctcgcg cgatgcctgc gtcaagctga tgaccgacgg catcctgctg gcggaaaccc
    20101 agaccgaccg ttatctcgcc gcctacgaca cgattatcat cgacgaagcg cacgagcgca
    20161 gcctgaacat cgacttcctt ttgggctatt tgaaacaact cctgccgcgc cgccccgatt
    20221 tgaaagtcat catcacctcg gcaacgatag acgcagaacg cttttcccaa cacttcaacg
    20281 gcgcgcccgt tttagaagtg agcggacgga cgtatcccgt cgaaatcctc taccgaccgc
    20341 tgaccggcaa agacgaagac gacgcagaag tggagttgac cgacgcgatt gtcgatgcgg
    20401 cggacgaatt agcgcgacac ggcgaaggcg atattttggt attcctgccg ggcgagcgcg
    20461 aaatccgcga agccgccgaa gccctgcgca aatccacgct gcgccgcaac gacgaaatcc
    20521 tgcccctgtt cgcacgcctg tcgcacgccg agcagcacaa aatcttccac ccctcaggcg
    20581 cgaaacgccg catcgtattg gcaaccaacg tcgccgaaac ctcgcttacc gtgccgggca
    20641 tcaaatacgt catcgacacc ggcctcgcgc gcgtcaaacg ctattccgca cgggcaaagg
    20701 tggagcagct tcatgtcgaa aaaatctctc aagccgccgc ccgccaacgc tccggccgct
    20761 gcggacgcgt ctccgcaggc gtgtgtatca gactgttttc agaagaagat ttcaacagcc
    20821 gccccgaatt taccgaccca gaaatcgtcc gcagcaacct cgccgccgtc atcctgcgca
    20881 tggcatcgct gaacctgggc gatgtggcgg cattcccatt tttagaaatg cccgattcgc
    20941 ggtatatcaa tgacggtttt caggtattgc tggaattggg ggcggtagag gccgtctgaa
    21001 aacaagtagg atatacactt ctacctgaca aagaccaaca aacgataaaa ctacggcaca
    21061 tctagatgcc caacctacaa cattgcacac ggtaaaaaca tgaaacactt actcatcgat
    21121 tttgaaaacg tccagccgca aaacttagac aaattaccaa ccgaaaatac ccatatttgg
    21181 ctatttatag gtgtattaca caaaatgtta cctattagtc tggtgcaatc cctactacgt
    21241 ttcggcgaac gtgtccatct tgtccagtta caaaaaacgg ggaaaaacgc attggatttt
    21301 tacctgtcct attacctcgg acaaattacc gccacagacc ccaatgccca aatcggcata
    21361 ctctcgcgtg atggaggata cgatgttctg gtcgaacata ttttgaaaaa ccaccaggcg
    21421 aagggtatcg tgcgcctagc caatatagat gaagtacaac atcagaaaat tactaccgaa
    21481 ccgccgtcag cattgctgga aaacactcct cagcctgaaa ccaccctcaa accacagcaa
    21541 ccattaactt cctatttcca agcagcccta actgcactgc gccgccccga cgctttccgc
    21601 ccctgccgcc tgcataacct gcgacaaaat ctgcgtaagc atattttgag tgatttgttt
    21661 aaagaaaaaa ccgatgaaga atgcgaaata accactgcta acgttatcaa taaactcaaa
    21721 gcacaaaact tcatcagcat tgatgaacag gaaaccgttt cctaccatct cagtgataat
    21781 gatttgttac aaagaatcca acgccatatt ttaagccaac gtcccaaaac ctacgctgat
    21841 tttcaagccg tcgtgcaaaa ccgagcagat gcacttcact taacagtcgg taccaacgac
    21901 attcaatcct ttgcgcgaca tttgcgcgac caaaacctga tccgccaaaa caatggggaa
    21961 attgaatatg caccgtttac tgaacctaaa ccacagccaa cgcccaagca gcctaaaaaa
    22021 accgcatggg aacctgatga aattatttgg aaaaaagtga ttgccgcgtt atcgttaaag
    22081 aaccgtccta ataaaaccaa aactttacgc aatacaatcc aggcactcac aaaatccaat
    22141 gcacaagaaa ctgacaaact gctacaacat ttacaagata cccaagtcct acgtattgac
    22201 ggtactaaaa ttgtgtatat caagtaggat gggcatttat gcctgaccta caaaaccaaa
    22261 aacaacctac cacccttaat caactccaca aaccctcttc agacaacctc gttttttgaa
    22321 aaacaatctg taaacagata actgctgaag aataccgttg ccgagcccca aaacccgtac
    22381 tgcaactttt attgtgaact tcccattatg aaaaaatccc ttttcgttct ctttctgtat
    22441 tcgtccctac ttactgccag cgaaattgct tatcgctttg tattcggaat tgaaacctta
    22501 ccggctgcaa aaatggcaga aacgtttgcg ctgacatttg tgattgctgc gctgtatctg
    22561 tttgcgcgtt ataaggcaac gcgtttgttg attgcggtgt ttttcgcgtt cagcattatt
    22621 gccaacaatg tgcattacgc ggtttatcaa agctggataa cgggcattaa ttattggctg
    22681 atgctgaaag agattaccga agttggcggc gcaggggcgt cgatgttgga taagttgtgg
    22741 ctgcctgcgt tgtggggcgt gttggaagtc atgttgtttt gtagccttgc caagttccgc
    22801 cgtaagacgc atttttctgc cgatatactg tttgccttcc taatgctgat gattttcgtg
    22861 cgttcgttcg acacgaaaca agaacacggt atttcgccca aaccgacata cagccgcatc
    22921 aaagccaatt atttcagctt cggttatttt gtcggacgcg tgttgccgta tcagttgttt
    22981 gatttaagca agattcctgt gttcaaacag cctgctccaa gcagaatcgg gcaaggcagt
    23041 attcaaaata tcgtcctgat tatgggcgaa agcgaaagcg cggcgcattt gaaattgttt
    23101 ggctacgggc gcgaaacttc gccgtttttg acccagcttt cgcaagccga ttttaagccg
    23161 attgtgaaac aaagttattc cgcaggcttt atgacggcag tatccctgcc cagtttcttt
    23221 aacgtcatac cgcatgccaa cggcttggaa caaatcagcg gcggcgatac caatatgttc
    23281 cgcctcgcca aagagcaggg ctatgagacg tatttttaca gtgctcaggc tgaaaaccaa
    23341 atggcaattt tgaacttaat cggtaagaaa tggatagacc atctgattca gccgacgcag
    23401 cttggctacg gcaacggcga caatatgccc gatgagaagc tgctgccgct gttcgacaaa
    23461 atcaatttgc agcagggcag gcattttatc gtgttgcacc aacgcggttc gcacgcccca
    23521 tacggcgcat tgttgcagcc tcaagataaa gtattcggcg aagccgatat tgtggataag
    23581 tacgacaaca ccatccacaa aaccgaccaa atgattcaaa ccgtattcga gcagctgcaa
    23641 aagcagcctg acggcaactg gctgtttgcc tatacctccg atcatggcca gtatgttcgc
    23701 caagatatct acaatcaagg cacggtgcag cccgacagct atctcgtgcc gctggtgttg
    23761 tacagcccgg ataaggccgt gcaacaggct gccaaccagg cttttgcgcc ttgcgagatt
    23821 gccttccatc agcagctttc aacgttcctg attcacacgt tgggctacga tatgccggtt
    23881 tcaggttgtc gcgaaggctc ggtaacgggc aacctgatta cgggtgatgc aggcagcttg
    23941 aacattcgcg acggcaaggc ggaatatgtt tatccgcaat gagtggcgta aaaaccaata
    24001 aagacaaatt tagatgatgt cggggaagat gcccgaccga caagactatg caaaatatga
    24061 aaaaccaagt acgcggatca ggcatggatg cacgatccaa tccggccaat gtttcagacg
    24121 gcctgcaaaa cagttcgggt catatcggta ccaacacgcg ttaccgcctg accaaactcg
    24181 gcgaacaaat ggcgcgcctg cccatcgacc cgaaaatcgc gcgcattttg ttggcggcga
    24241 agaagcacga ctgcatggcg gaaatattga tgatcgcgtc cgcgctgtcg attcaagacc
    24301 cgcgcgagcg gccgctggaa gcgcgcgatg ccgccgccaa ggcgcacgag cgttttaccg
    24361 acaagcaatc tgactttctt gcctatctga acatttggga cagcttccag cgcgagcgcg
    24421 acaagggctt gtccaacaag cagttggtgc agtggtgccg tcaatatttc ctgtcgcacc
    24481 tgcggatgcg cgagtggcgc gagctgcacc accagcttgc ccaaaccgcg attgaaatgg
    24541 gtttgaccac caaggaagcg gctttcagac gactttccga aatcaagcag cttacgtcgt
    24601 ctgaaaacca aggcgatcaa gacttatccg ccaaacgcaa acaaaaacaa ctggataaga
    24661 aacaacaccg cgcccaaatc cgcgccgcca aagaagcggg ctacgaacaa atccaccgcg
    24721 ccctgctcac cggccttatc gccaacgtcg gcatgaaatc gcccgacggc aacgactata
    24781 ccggcgcgcg cggcagccgt ttccaccttt tccccgcctc cgccctgttc aaagccaaac
    24841 ccaaatgggt gatggcggca gaactcgttg aaacgacaaa actttatgcg cgcgacgtcg
    24901 ccgttatcca gcccgaatgg attgagcagg aagccccgca cctcgtccgc taccactatt
    24961 tcgagccgca ctgggaacaa aaacgcggcg aagtcgtcgc cagcgaacgc gtgacgcttt
    25021 acggcctgac cgtattgccg cgccgccccg tgccttacgg caaagttgcg cccgaagaag
    25081 cacgtgaaat ctttatccgc agcgcgttgg tggcgcagga atgcgatttg aaagcggatt
    25141 tttttgtcca caataaaaag ctgattaaag aaattaccga actcgaacac aaatcgcgca
    25201 agcaagacgt attggtcgac gacgaagccc tgtttgcgtt ttatcacgaa cggctgcccg
    25261 atttttatac ggcggatgcg gtttcagacg gtctgcgtcc tgcaaatccc cagcaaactg
    25321 ccccctccta cgcgagggaa gaacggagag agggcaaaac agttgccgca caaaccaact
    25381 tctccgcaac cgcagcaaac cctctcccta accctctccc acaggagagg gagcagagtg
    25441 ccgcagcttc agcggtttca aacgacctgc atcctgcaaa tccccaacaa accgccccct
    25501 cccccgtggg ggagggtcgg ggagagggca aaacagttgc ctctcaaacc aacttctccg
    25561 caaccacagc aaaccctctc cctaaccctc tcccacagga gggggaacag agtgccgcag
    25621 cttcagcggt ttcaaacgac cccaaaccca aaaagcagcc tgcaccccca aaaggccgtc
    25681 tgaaacccct ccccctcgcc gacatccgta ccttccaagc ctggctcaaa accgccaagc
    25741 gcgacaatcc gcgcctgctg ttcctcagcc gcgacgattt gatgcaacac gctgccgcgc
    25801 acattaccga agagcaattc cccaaattct ggcaaaccgc agacggtaag ttcaaacttt
    25861 cctaccgctt cgagccgcac catccgctcg acggtgtaac catgaccgtg ccgctgaccg
    25921 tcctcaaccg cctgcacgcg ccgtcgctcg aatggctggt gcccggtatg ttgcgcgaaa
    25981 aaatccagct gctgattaaa gccctaccga agcaaatccg ccgcatttgc gtgcccgtac
    26041 ccgatttcat caccaaattt ctcgaaagca atcccgaccg ccaagcggtc attattcccc
    26101 agttggcgca ctttatcgcc aaaagcgcag gcgatatgcg gattttcgag caaatcgacc
    26161 aagacgcatg ggcggcgcaa gaattgcccg aacactgcta tctgaatctg ctcatcatcg
    26221 acgacggcgg acaagagctt gccggcggcc gcaaactgca cgaattgcaa caacagctcg
    26281 gccaagccgc cgccgtcacc ttccgcgaca acacccaaga attcgagcgc gacaacgtta
    26341 ccacatggga tatcggtacc ctgcccgaat ccatcaaatt cgcccgcggc aaacaacagc
    26401 tcaccggcta cctcggtctg caaaaagaaa aagacggccg catcgccctg cgcctgtgtg
    26461 acaccaccga agctgccgaa caggcacacc gtcaaggcgt catcgaattg atgaagctgc
    26521 aattaaaaga gcaggtaaaa gacctgaaca aaggcatcca aggctttacc caagccgcca
    26581 tgctgctcaa acacatcaac gccgacaccc tgcgcgacga cctcacccaa gccgtttgtg
    26641 accgcgcctt tatcggcgaa gacgagctgc cgcgcaacga aaaagccttc aaagaacaaa
    26701 tcaaacgcgc ccgcagccgc ctgcccgccg tcaaagaagc cctcagccgc tacctgcagg
    26761 aaaccgccgc cgcctacgcc gaactgaacg gcaaactcgg caaacaccca ttgacccacc
    26821 ttctaagact acgcctgcaa accctgctcg ccgccggctt cgccacccgc accccgtggg
    26881 cacaatggcc gcgcctcccc atctacctca aagccatgac cctgcgcctc gaaaaataca
    26941 gcagcaaccc cgcccgcgac gcagcccgcg aagccgatat acaggaattg gaacaaatgt
    27001 ggcaggaaaa aaacgacggt ttggtgaaac aaggactacc cgtttcagac gacctcaccg
    27061 cgtttaaatg gatgatcgaa gaattgaggg tgtcgctgtt cgcgcaggaa ttgaagacac
    27121 cgtatccggt gtcggtgaag cggctgttga aagagtggga ggatcttaat taaaggaaga
    27181 tgccgtctaa ttttttcaga cggcatcttt tatttccctg accaaccgat ttaatcgctc
    27241 cctacaaata gctttatttt ttaattcgca ctcccaaacg acaatcactt tgaaaccgat
    27301 cttttccagc tcggtttcat ttttaatatc acgttcgcaa ttttttgtga ttttttccaa
    27361 ccaaaaatcc gtattgcttt tcgggatatg tcctttatta caagaatgcc cgtaccaaaa
    27421 acagccgtgt ataaacacga ctgttttata tttcgacaaa acgatatccg gtttgcctac
    27481 atagcgttta tcgtttttcc gataacggaa accttggaaa aaaagaaatt tccttaccaa
    27541 tacttccggt ttggtttctt tgctatggat ttttaacatc acgaaagaac gtttggatgt
    27601 agtgaaaata tcggtcataa attacttgga agataccacc ttcgatcgtt tttttgtctg
    27661 ttccactttt cttaacaaat acgaaaccaa gcttattaaa tccaaagcat ccttatcatt
    27721 aaaaatacta ggagataaat ttgctcttaa ctcatgttgt acaggatttc taaatccagt
    27781 ccatgttccg catgcaagct gttcaagacc atcttctaaa ttttgttccg ccttcgtttt
    27841 gttattcgtt aatagaagta tagaattttt actcccgaaa gcttgttcaa tcaattctct
    27901 tccgtctatg ctatgaagac ctgtttgctt ctgaacctta tcttcgaaaa ctttaactgc
    27961 ctcgattgca gcacttaaat aatccttctt ttcattgtaa aaatcattac aggcagtatg
    28021 caaatcctga tgcaaatgcc gccaatgata gtaaggatat tctggaataa tactatgtaa
    28081 ggcagagatt acttccgctt gctctttatt tgtaagctcc gaatcttcca aacgattcaa
    28141 aataacttct actggttccc tttccttttc tggcaatttg tttttccaat cttcgatatc
    28201 aactccgctt tcgcctttca cttcgtcatc tttctctttt tttcttcttt ttctccaatc
    28261 ttgcccgatg gctcggacga tctccccaag gaaaacacgt aattttgagg tttttgaatg
    28321 cttccagtct aatgatgtcc tgtcggtagc aattaaatct tcatcgtcat tatctataaa
    28381 gtccacattc agccatccag tcaaatatga atagaaatgg ctggattcgc tatcagtgaa
    28441 aaactcgggc atatttacca ttctgccgtt ggcaaacaag gtaatacctt tcatattatt
    28501 ctttaaaggt ttttccgttg taatgaactt accttgtatt aatccgctgt attcatactg
    28561 ttcgaatttt gaagataacg atgaaatatt ggttgctaaa tcctggtatt cccattcgaa
    28621 ttgtggggta acaagatcat attttagatt tccatcaatg attttaggtt cagagccgtt
    28681 caagcttact ttgattttaa aatcctgagc cggaaaatca aacaagcggg aaagatgctc
    28741 tacataatta tctaacggat atccttgctt tttcgtcagt tcggttaaag ttatggttgt
    28801 cccgctttcg atattggatt caacagattc ttttcgaaac tccggttgat aaataccctt
    28861 gcttcttcga atctctgcat aatccaaagt aaaagtaacc ctttcgtttc cctggatagt
    28921 agaaatttca attttgttgc caagcccgaa taatgccaat ttaccaaggc cttttttacc
    28981 cgttggaatt cttccgcacg gggaggcttg tttttcttcc cttctgttcc gaccgattct
    29041 caaataaaaa tcattgattt catcgaagct cattcctata ccattatctt taataacgat
    29101 tttatgctcc ggtttatcga ataacctaac ttccacttcc gtagcacatg catcgtaggc
    29161 attcgctatc aactcggcaa tcgcaggaac agtgtgcgaa tacatcttta cacccaaatg
    29221 ttcgattacg gttggctcga aactcatttt caatttttct tcagacatgt tttctccttt
    29281 tctaacattc gatattatca acaattgccc tgcctatttt ttccgcatac atcggaggaa
    29341 cggcattacc aatcaaacgg gctatcttgt ccctgctgcc cgctttaaag acatagttac
    29401 gagggaacga ttgcaatgtt gcaccttcgc gcagtgataa cgccctatct tcttcaggat
    29461 gggcaaacct cccgttggaa atactaaaga atttggtcgt tatggtaggt gcgggcttat
    29521 cccaccatag cctaccaaac gtgtctttaa aactattgtc ttttccgata aaacattcca
    29581 gctgtaattc gggaatatgc gcaaaagcca aacggtttcc tccgttttta ggtatcaaag
    29641 ccaatcgttt caagttgata tcagataatc ccgcacagct atgcataaaa tccgtttcgt
    29701 cttggtgtcc tgcaataatt ttgggaaagc cattttccat tcccaaaaca tcgcgtaccg
    29761 taagccgttt gcccgaatac ttgactggtt ccagcttttc tttggttatt ctgtttgcaa
    29821 ttaacgtaaa tcttttacgg ctttggggaa ttccgtaatc agccgtattg tgaataccga
    29881 aatggacggt aaatcctttt tcttccaata actttatgaa attatcaagc ctgctttctt
    29941 tcattctgct cagaattccc ggtacgttct ctacaacaac atatccggga ttgaaatatt
    30001 ctacaaaccg ctgaaactcc aaaagcaaac tcttggattt ttccgatttt cttttgtctg
    30061 tttgaataac actccaatat tgacaaggac tacaacctat aagaatcaaa tcatcatcat
    30121 tcttttttaa cccgagttct ttttccaagg tttcaggctg caattcaaaa acatcagcct
    30181 gtataaattt ggcatttata ttggcttcat atgtttcttt gcagctgggt tcgtaatcta
    30241 tcccggctaa aacctgaata ccggcggatt gcattccata gctcattcca cctccgccac
    30301 agaaaaaatc aaccgccttt aacttactgt ttttcatttc tatttaaata attagactag
    30361 tgagaactaa ctttatataa catataattt attgcttatc aaaaataatt tgagatttta
    30421 ttaaaattca tgcatctaaa taagcagaac ttaatttcct ccaaacgagc ggatgtcttc
    30481 aaatgcacac ttcacagaaa ggtcgtctga aacccatttt cagacgacct ttttccactt
    30541 cccaaaacaa gaattcccac cccctcccca aacgtcgccc gaaccagtaa actccccata
    30601 tttcaacaac ttcttggaag ccaaccatgc cccgtatcca ccctcctcac ctgttcccgc
    30661 cccatatcgc cgaacgcggc cttttgtatt ttcagcaggg caaggttcgc gatgtccgaa
    30721 aaacttccgc cgggcgttat cgggcggagg tatgcggttt ggaaaactat tgggtatggc
    30781 tgaagctgga tagtgatttg tatattaaag acgaaagctg cgattgccct tatgtctaaa
    30841 ggtgcaaaca taccttaaat tactatatta cataggcaaa atacaagcct ataacgaatt
    30901 ggaaacaaaa tgccgtctga aaacatcttc agacggcatt ataaaatctg ttcacctttt
    30961 cagatgagta atgtacaccc ctatacaatt tttgctacta taccccataa atccacggct
    31021 aaagatatcc ttattatgtc ctatgattta tcgaaacgac ttgtaatcgg cttagcatca
    31081 agtgccctat tcgacttatc cgaatcggat aatatattta gaatggaagg ggcagaaacc
    31141 tataggcaat atcagagaga aaaacaaaac catcccctaa aaaaggcgtt gtctttccat
    31201 ttattaaaaa acttctgtca atcaatgaaa taaacccaaa cgacccaacg attgggttta
    31261 ttcttttatc cagaaacaat ccagatacag attacgagtc ataactatag gcttaatatt
    31321 acacgatttt cattccatca aggcggaaaa ccgcacaaat actgaaacac tatcgatcga
    31381 tttgtaaaca agcctactta agtaacttgc agtccttatc atttccttta aaataatcca
    31441 gcccgtcact acacgaactg gcggacttct tgcaaataaa ggttactaga ttttcattca
    31501 tcttaataat aaaaggattt ttatctttat ctatggctac cgccttcaac atgaatttac
    31561 tgtctaaggc cccgcgcgcg attccattca aacggataca aaagccttct gcctctttaa
    31621 tcggcaaact tggccatttg gtagatgttt gtttaaatct cccattctgc agataaaact
    31681 tttccataaa atgtgcattt tctaacaagg ctgcccgcac tgtatttatc tttgctttct
    31741 caacataatt gcgatagctc ggataaacaa ttaaagcaag tacagacaat atcaagacca
    31801 ctgatattaa ttcaaccagc gtaaaccccc gattatcagt cattacttta cttccaataa
    31861 gaacagatta ttcaacatat ttctttgaac agacttacta tcccattcag cagtatgcat
    31921 atttcccac
//
LOCUS       DAWKQB010000007        29307 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.007, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000007 DAWKQB010000000
VERSION     DAWKQB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.007"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..297
                     /locus_tag="WJL14_000216"
     CDS             <1..297
                     /locus_tag="WJL14_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655347.1"
                     /translation="AGLQIGLQKANEQEPFIAAVAETLSRTMWGYVMRMDARFEVTFI
                     VDLGHVAFEHQFTAVKQALKEKFEVEADGDALTVESDRLPYGIAACRVVFGDVL"
     gene            294..575
                     /locus_tag="WJL14_000217"
     CDS             294..575
                     /locus_tag="WJL14_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655348.1"
                     /translation="MSGDTPITVEYVFGTLVSFLIALLWYWVKSISDGLKEARKDRDE
                     LLGRLHSVETSYQTKAEAKDNRNEILNLLREIKADLKEVGQKIERKADK"
     gene            575..790
                     /locus_tag="WJL14_000218"
     CDS             575..790
                     /locus_tag="WJL14_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655349.1"
                     /translation="MQNQDPILRALAEINGKQDKLLQNQERMDAEIKKIHADCRRTSA
                     TTGAAAGAISGGIVATGIAFARAKLGL"
     gene            793..1299
                     /locus_tag="WJL14_000219"
     CDS             793..1299
                     /locus_tag="WJL14_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7655350.1"
                     /translation="MAHPKATRDKLRALYCNGEQSLETAAALCGVSLGTARRWRDEAK
                     AQGDDWNKLRAAHTLAGGSIDEIARAMMTSFLVQYQATMTMLQDAEVEDLPPSKRVQL
                     LASLADAFTKTVAANKRVLPETSQLATALELLQFLMVFVQEKHPKHLAAFVEVLEPFG
                     AEVERKFG"
     gene            complement(1296..1490)
                     /locus_tag="WJL14_000220"
     CDS             complement(1296..1490)
                     /locus_tag="WJL14_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655351.1"
                     /translation="MKSIDLEISKLLDAGKYTPSEIQDLLEEQGFKISLKKLADHLDL
                     LVAIGVAGKHSDDTFTSRLN"
     gene            1502..3121
                     /gene="terL"
                     /locus_tag="WJL14_000221"
     CDS             1502..3121
                     /gene="terL"
                     /locus_tag="WJL14_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7655352.1"
                     /translation="MKNKDFLKSLSALAANLRQVIEAEVDGFDASPTAVAERRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSDLHRFLFRRLPEIVESPEGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGRKHYCVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFADVCGQGRV
                     WQAGTIVTANDIKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWKGIKFKAMLKWPDRMDLW
                     DRWEELYRNEGELVADAFYQTNKAEMERGAETSWAARGVLALMKIRARDGHAAFDSEY
                     QNDPVSGEDAPFAQAMQFWAELPADLVYFGALDPSLGKAGASRDPSAIIVGGYQRSSG
                     KLFIVEAQIKKRLPDLIIEDVIRLHAQYKCKLWFVETVQFQEFLKDELVKRSAAKGIP
                     VPARAVKPIADKLLRIETLQPHMANGLILLHANQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGATANSAPVEYMAVTKHGTDGGFGSGAW"
     gene            3200..4759
                     /locus_tag="WJL14_000222"
     CDS             3200..4759
                     /locus_tag="WJL14_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7655353.1"
                     /translation="MKNIFSSALSKILPGRYTPKSTPQTAEITQNTQTHEHPSKGLTP
                     QKLHGILEAAERGDMKAQSELFADIEEKDGHIFSEMSKRKRAVIGLDWRVMPPPNSTD
                     AERRLAEEVKGWIERLPDFEDMMFDLLDAVGHGFACVEIEWQQIGGLWLPKNFIHRPQ
                     GWFKVDGADNVRLAKQDNPDGEELWAFGWLVHKHRSRSGLLVRGGLMRTLVWPYLFKN
                     YSVRDLAEFLEIYGLPTRLGKYAVGADETDKTTLLRAVKEIGHNAAGIIPETMNIELL
                     NAANGSSEPFMAMIDWADKTSSKAILGGTLTSMADGKTSTNALGQVHNEVRHDLLVSD
                     AKQLAGTITQQLILPLLRLNKGNVDETCLPRFQFDTQLPEDMAVYAESLPRLVEMGMK
                     IPLAWAQEKLAIPLASDDEPVLALQTAESEGVKVAPLSYRRVALSRQGEILDMGQAAI
                     DNAGLGKIALPEHIEPFLRGLGQALAEGDSYEEVQERLLRAYPHLDSAEFQTALARVI
                     FISDLWGRLNG"
     gene            4752..6098
                     /locus_tag="WJL14_000223"
     CDS             4752..6098
                     /locus_tag="WJL14_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ7655354.1"
                     /translation="MADLGFAFGLEPEAAVKYFEGLGYHIPPDWDVKWNEAQTKARTI
                     AGIHRQDIVGEFHAAMYEAAKSGKSFEAWRDEVQGRLKAHDWHLLKDGDIVDGDTGEV
                     LGRGITKHRMETIFRTQMQSAYMAGHWQAFEEGRDDAPWLQYSAILDSRTRQSHAAAH
                     GAVYHIDDPFWDYFYPPNGFNCRCTVRALSDSDLKRRNLLPQKAQLEDTEVVVNRKGD
                     TRPAKAVKLPDGRRFYTDAGFQHNVGKSHLANLGQLQMQRAVELPPKLASVSIQTALK
                     QPDLMRAVSQQAAQMVRRVDAEKVARGKTLYVGALTLPVLDALAARKIYPQSAVIAIS
                     DERVLHALRDKKVKPLPVSFWEKIPELLQEPEQILLGKAGRNDDAKQFLAFVYPLSAG
                     KGKLVVTLDYDVKTRHPLTGKKEHLTLNMVNTGMIAETDKQVDSLLYGYETIWKKP"
     gene            6202..6699
                     /locus_tag="WJL14_000224"
     CDS             6202..6699
                     /locus_tag="WJL14_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7655355.1"
                     /translation="MIEIKINTDTLQNSLNAAARHTSHTKPLMTRLARIMRNAVLENF
                     AAGGRPAWAPRKYPTAREGSGLLQASGRLRNSITPSSTNDTAVVGTNVEYAAIHNFGG
                     KTSPHLIRPKKGKALKFGGRFAKQVNHPGSNIPARPFMTLQPEDEKALSDAVAEYLAQ
                     AIQGR"
     gene            6908..7972
                     /locus_tag="WJL14_000225"
     CDS             6908..7972
                     /locus_tag="WJL14_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7655356.1"
                     /translation="MRAMDTKTLLAALSAAHVGGSDGLIKIVPKGQFAPVDGRTDTGV
                     PHWTMSADLAQQIIAAFDAAQTDLVVDYEHATLKAAETGQQNPAAGWISKYVWDDERG
                     LMGEVKWTQRAKDMIDSGEYRYLSPVLEYDTLGNVRGLHSVALTNSPALDGMALAALS
                     RQNSINPKQETSMNKEALIKLLGLAADADDKAIEAALAEAQEKLGGKTLAEALAAPKE
                     EPQGSEGDKGDAGKPEDKPQGGNADDGEVAELKAQVAALSKKVIAMEVGGTSDGLIRA
                     ALSDGRLLPHQEASARQLAAKDPEAFKALIDGSLALAALSKTQTGGKGGADGTSALTA
                     EEAAVAAQLGISAEDYAKAK"
     gene            7994..8896
                     /locus_tag="WJL14_000226"
     CDS             7994..8896
                     /locus_tag="WJL14_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEZ7655357.1"
                     /translation="MIITPDTLKALFTGFKKNFQDGLKMAESQYKEIATVIPSSTASN
                     TYGWLGQWPAFREWVGDRVFQDMKAHGYAITNKHFESSVKVNRNDIEDDNVGIYAPMM
                     TEMGRASAVHPDELVFALLKNAHATLCYDGQNFFDTDHPVYEKVDGTGQASTVSNIFA
                     GSEAAWYLLDTTRALKPLIYQERKAKQFTAMTADTDEGVFMRNEYRYGVDGRCNVGLG
                     FWQMAAKSQEKLDAAGFEKAYNAMVSLKGDGGRPLAIRPNVLLVPPALENAAKELVEG
                     DRLANGAYNPNKGKAKVIVSPWLL"
     gene            8934..9407
                     /locus_tag="WJL14_000227"
     CDS             8934..9407
                     /locus_tag="WJL14_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655358.1"
                     /translation="MAKEKNGQEVGATVDVQPEEVTVTAALQAEIEALRAELDKANAE
                     IQVAQAELAAAQERNAKLERLLEAGLAPGGISDGETLTPEAEAAFYTGSGAPAADAEV
                     VAIKSKHGHAFFRAGYHVQPNWTFVRRADFEPADFERLIGDRMVEAREALPTDAS"
     gene            9404..9829
                     /locus_tag="WJL14_000228"
     CDS             9404..9829
                     /locus_tag="WJL14_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HEZ7655359.1"
                     /translation="MSYAAVADLVARFGEATITGLTDLSRKGAVDETVAQQALDDAAA
                     EIDGYLMNRYTLPLPKPLRILTVYCCDIAVYRLCTGKRQLTEDIVHRYEAAVKFLQLV
                     AAGKVGLGVTEPAGEKPAVQGNGVMFTTQEKVFGRDSVY"
     gene            9813..10481
                     /locus_tag="WJL14_000229"
     CDS             9813..10481
                     /locus_tag="WJL14_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7655360.1"
                     /translation="MIASIEQAIRQRLSDGLGQMVTGVFTYGGEFDGEGLAQVVNQFP
                     AVWVMFAGIKDTIRHDTRGSRFKAIGQFTVLVGDRASGSEADSRFGGLHRHDVGTYRL
                     MQTCRHLLTNQSLGLQIDRLQPGAAKSLFSRQMEQDAVSVFALEFETYWFEDALQDGD
                     WPRPAVSDGQQAKVYADVSEYQGRTEPEHPDFKGVNLELRIPPKTPDQPADMAATVET
                     KVKP"
     gene            10478..10666
                     /locus_tag="WJL14_000230"
     CDS             10478..10666
                     /locus_tag="WJL14_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7655361.1"
                     /translation="MNETIKVRAADGLQVPLAGKPHEYITDQETVTVPDAAYYRRCIE
                     YGDLVVVTEDTQPAKGSK"
     gene            10666..12093
                     /locus_tag="WJL14_000231"
     CDS             10666..12093
                     /locus_tag="WJL14_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7655362.1"
                     /translation="MASANINFEKIPASTRKPGVYAEWNTKLAVRNLPTNKQRVLIVA
                     QHNNPALGELTELENVFSAADAAAKYGAGSMAHLMVTAAIKAYAYADLSLITVADNKA
                     GVAAGGKITLSGTANTQGVLRVSIANADTLTIGIGAEDTAATVAAAVKAAIDAVPDLP
                     VTATVAEAVVTLTAKNKGTAGNAIRIKTSNTAEGITAAVTAMTGGDANPDIAAALNAV
                     VAEGHHIIACGINDETNLLKLRAHLDTVASPMEKRWAICVYGQTGTLAQATTLAGRLN
                     HGHIVSAWYRGTPSLPCELVAAFAAVMASEEDPARPLNTLALNSIGVCESKDKTMRTE
                     QENALYNGVTPIETSPAGTQAQIVRAITTYTKTANGTADESLLDVTTVRTLIYVSRAC
                     VDRIALRFPRDKLSDRTPPRVRSELIDVLMCCEELEILERVEENLPKLIVERDLQNTG
                     MLNCRIPSDVVNGLHVVGMVVDLYL"
     gene            12107..12481
                     /locus_tag="WJL14_000232"
     CDS             12107..12481
                     /locus_tag="WJL14_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7655363.1"
                     /translation="MSTEYVGSVILYLGANEVQVTKIDVKDNTGKKPVKTMNRTRRVK
                     GFTRGVGQYDITFTAVVPTDGTAVDWAKIDDAKISLVPDVEGARPTSYLGFCATEAGD
                     SYTVDNELVVDVTGFAIRKVLE"
     gene            12657..13043
                     /locus_tag="WJL14_000233"
     CDS             12657..13043
                     /locus_tag="WJL14_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7655364.1"
                     /translation="MGLTKFGAAVREARRQTKQTLQTMAATLGTSPAFLSAIETGRSK
                     VPMDFVKKVEDFFENLGQPIDGLKQKAMVSNENVSLSGLSLQQQMLVAGFASSDFSKE
                     QLDKFAELLRTIHHKDSQKEGDDAAN"
     gene            13030..13494
                     /locus_tag="WJL14_000234"
     CDS             13030..13494
                     /locus_tag="WJL14_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEZ7655365.1"
                     /translation="MQQTNYSMRGVRVGMLSEETIQFFANEAAKFLVVDKRTRKKMDA
                     FMEMLEEYGIVIDVVADSEWLVITNAMCHNGTILMPNSLYTRICNGEDEAIFIFFHEL
                     GHLLLGHKAMLHYSEVLPTKQEDSEWQADEFAKCILRNMGIKYVPEQLSLRF"
     gene            13676..13855
                     /locus_tag="WJL14_000235"
     CDS             13676..13855
                     /locus_tag="WJL14_000235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655366.1"
                     /translation="MMATIFTIGGAIMAMTKAPVTPDLVDDTGAYIFRKSFTTKSGKK
                     IVSKNGKPFKIPVKV"
     gene            13984..14373
                     /locus_tag="WJL14_000236"
     CDS             13984..14373
                     /locus_tag="WJL14_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655367.1"
                     /translation="MSEFSPELTRAIEDYELRVSPDLKTVAGRLKYGIGVIDGEMHHD
                     FSMHLLTVREDMAIDPQLEGQPRLVAAYAASLDKLGGLTAESLTPDLLLDEMAAADFD
                     ALYWAQELLQKKRLCPHPAPTVTDTPS"
     gene            14331..14579
                     /locus_tag="WJL14_000237"
     CDS             14331..14579
                     /locus_tag="WJL14_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655368.1"
                     /translation="MPAPRADRYRYAVLKLGRYGITPAQIDGMTQPELEGWLKQADLI
                     ERGRAAPVVMPWFIPANVKPSAATGGQTQTFISKRKKK"
     gene            14576..16625
                     /locus_tag="WJL14_000238"
                     /pseudo
     CDS             14576..16625
                     /locus_tag="WJL14_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            16625..17992
                     /locus_tag="WJL14_000239"
     CDS             16625..17992
                     /locus_tag="WJL14_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7655369.1"
                     /translation="MAWKDTLLDAGFKGVTFDVIDDTLRGTHALAEHEYPFVQGADIE
                     DTGVSAMDMALTAVLWGEDYEGRLQNLLNVLCETGAGELIHPIYGSVPDCVVADFEVA
                     HNEENPDYCTVRMTFKQSVKAAPFFDRDLPIALADEVDFLADLAAWQGFEVFQTALNK
                     IQKAQSRWNAFHATVLMVVGVLYGQVNGIFTGGLNLLNSPRVLVAELKSVFGALANMH
                     NVGKSGLDGWRDMVGGVSKAAATPWQVSRGTEGSVAAVDLIQRAKPEDVAAFTALTAT
                     VGACALAEQAADILAVQLNEPTLTPVEISRLLADTRAALQRALAAQRILAMMLADETK
                     ADKLAYCLLRLYQTPVDSADDVYQRIEVAGLLPQAPYLETAAALTESLRNTAHKLQKQ
                     AFTVINMKPPLVQKTVTCDIGLHLLAFEWYGDYSRFGELLRLNPQIRHPNFIEKGTVL
                     NAYAR"
     gene            17976..19115
                     /locus_tag="WJL14_000240"
     CDS             17976..19115
                     /locus_tag="WJL14_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7655370.1"
                     /translation="MPTPDNTVTLLINGKTHGQWTNYDIVSDLLTPADDFSVMLGRPV
                     DAKPDAVRAGDKVEVRVGGDTVLSGRIDRVQTVTEKGGKTLTIQGRDDAGVLLDCSAP
                     LFNAQDMDLNQIIEKIVKPLGLAKIRIDAAKTDKTHKVQIEPGSRAWDALLEYAEANG
                     LWPWLEPDGTLVVGGPDYTAAPVAELVLRTNGQNNNIKRLEVNRDMAARYSEVTVLAQ
                     SHSGKNNIKATAKDESVKLHRPLIVTEPDIDSQAQAQRKAKKRLADSRLEGLTITATV
                     QGHRTDDGTLWQPGQRINVLSEPDGIDAVYFLMARTFIGGRGQPTETVLTLKEDGAWV
                     LDADPPKKSGKTKRPSESRKANGHAAAKPKKRRQAKQAGQELQVI"
     gene            19116..19745
                     /locus_tag="WJL14_000241"
     CDS             19116..19745
                     /locus_tag="WJL14_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEZ7655371.1"
                     /translation="MDAKTIDNRIRRAFNGIRQAFRGKIARVKAAGGVQQIQVEGLEG
                     ETVQDLEHAENFGFTSNPPAGSDCVVVPLGGKTSHGIIVTTTNGAYRITGLAEGETAV
                     YNADGAKMVLKKGRIIEIDCETLNIKAPGGVNIDAPNVGCTAQITAEGQINGNGGMAV
                     KGGSGTSFTGNVQMVGDLDTTGKLTNNGKNVGSDHKHNGDSGGTTSDPL"
     gene            19806..20159
                     /locus_tag="WJL14_000242"
     CDS             19806..20159
                     /locus_tag="WJL14_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HEZ7655372.1"
                     /translation="MDALLNPQTGGYVVNQSAQSIENELYIRLVTPLGSYWADRTLGS
                     RLHELRRQKHLKRIEVLAKQYAEQALQPVIQSKRAQSIQVTASAPQHGWLKLHIEAVD
                     AAGDTVTLNHKVAVI"
     gene            20156..20641
                     /locus_tag="WJL14_000243"
     CDS             20156..20641
                     /locus_tag="WJL14_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7655373.1"
                     /translation="MTQALNLEQIRANYLRDLQNQNPAAHVHAGSDNHVRATAIAAVG
                     EGQYQHQEWILRQAFADTADSAYLEKHAAKYGIYRKTATFAGGKVRVRGAVGATVPVG
                     QQINVGDKVYLTAESAVISALGSAEIAVIATVAGSAQNQTAETAATLQSVPAGIDSSA
                     V"
     gene            20644..21219
                     /locus_tag="WJL14_000244"
     CDS             20644..21219
                     /locus_tag="WJL14_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ7655374.1"
                     /translation="MVGGTDAESDESLLARYEERLRRPAAGGNQYDFRNWCLEVPGVV
                     DAFIYPLRRGNGFVDAVILGENGIPSAETLAAVQAHVDAVRPVTRKNGFLALAPSIQT
                     VNVAVTITLSSGTDTDTATAAIKSAVNAYFDALKPGDPLIKSQLETLISEVYGVRDRV
                     LTTPVGNIKPQESAEDIYWLRPGSINVEYTT"
     gene            21216..21782
                     /locus_tag="WJL14_000245"
     CDS             21216..21782
                     /locus_tag="WJL14_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7655375.1"
                     /translation="MIHQTLLAAMRPPVSYDTVGETAEIKAEAGVFDIVADHAEGVKN
                     APFPDAENDYLYRWEELLAITPPAGANTQQRTDAVLAKLNALGGLSIAYFTAIAESAG
                     YTVTIYEEDQFRAGESCAGDCLNTEDAIWRWCVDIADGKATAYIFRAGQSRAGDRISV
                     YTDPIIETMFEELKPAWTYCRFEYEEEV"
     gene            21785..24067
                     /locus_tag="WJL14_000246"
     CDS             21785..24067
                     /locus_tag="WJL14_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEZ7655376.1"
                     /translation="MDLIQTPNKQFVDGDRRTPGTPVPAWWLNQLQGELYSILNAVGI
                     EPNKADHAQVLSAIKTLAADASQVASIDALRKYSGTGYVNVNAYHANTTVGGGVFVAD
                     KADKSTADNGCTVIVSTDGTRWKRVFSGMLNLHDFGYVASKNNALSTLNAAESAALDV
                     VVDCLGLSIDTGNTYPQKNKYTNGKFVINGKTVDVQYQPIRSGIGRFISGTGAAANLK
                     SNEWTGAGLIVIGEGAMEQMEKCVSSIAIGDRAQGFSKVSRDNIAIGADSLINVQAAT
                     EWYDQSRMEGTRNIGIGGNAGRGITSGYSNVSIGRNAGQGLGEGSSNIALGAGAMAGT
                     APVGFSGDIEVFWPSSTSRTIAIGEAVLQTYQGRAAQTAIGANAARNTKKAEKVTAIG
                     SAAMENLERNRAPNGGDVVWTGTEAGTYAQSGKNITLTFPNIRGAQATYWVGIRLTSG
                     TAQTLQNDVVPAQVVSVNGNTLIIQSSKELTATGAAELKYVYSVNSTATKNEELTIIG
                     ANAMNKALTAGYSTIIGVDAALLGDNYQKTTAIGASSLRTGSHISTTAIGYWVIPLAS
                     SEKCVAIGDSAGYRNVQGDFLTGKITNSIAIGYGARINGDNEIQIGTTGQTLYAPTAV
                     NIRSDGRDKADVKPLTNGLDFVMKLKPMTGYYDRRDSYVDELFKDLPADERADKVREW
                     WANPIKDGSHKEDRLRHWFIAQDIAALEDEYGRLPMVNKTNDTYTVEYETFIPVLTKA
                     IQEMAARIETLETEMKESKK"
     gene            24064..24687
                     /locus_tag="WJL14_000247"
     CDS             24064..24687
                     /locus_tag="WJL14_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655377.1"
                     /translation="MTRCVIDQDGLFVEEQYFDDGRQSIEAEMPDLAQYQAAQWDGQG
                     WKIIPDYRGCVVFAGGQEQVWDKLGDLPDGVSLTPPESVNIDGLKSVKLVALNAAAQA
                     FINKHAGIDSVPEFEFASWAIQAAEAKAWQEDKAAPTPVLDGIATARGMSADTLKAAA
                     LRKTLAYEQLAAHVAGQRQALQSKIEAAKTQAALDKIAVVFTLPEAV"
     gene            24688..25176
                     /locus_tag="WJL14_000248"
     CDS             24688..25176
                     /locus_tag="WJL14_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655378.1"
                     /translation="MVQVYLALYKGKAAINTPRDVVKRIADSVVRLATCSPYSHCEIA
                     VKHPRDGLFDCYSSSARDGGVRIKTMPLPADKWDLIPLPQSVAISASRLFHRTHGAGY
                     DWLGAIGVVLKSPHSKSRWFCSEWCAYVIGYTNPCRYSPQTLYAAVSTKDRPSEKMEE
                     TK"
     gene            25524..26363
                     /locus_tag="WJL14_000249"
     CDS             25524..26363
                     /locus_tag="WJL14_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ7655379.1"
                     /translation="MMQKYHSTAPLPFVGQKRYFIKHFTKVLSQIPADGKHWTIVDVF
                     GGSGLLAHVAKRIKPQARVIYNDYDNYSDRLRHIPDYNRLREQIAQIVGGIPKGSRLD
                     PERTRSVQQTITNFQGHIDVRVLSSWLLFSAKQANSLEQLLGFEFYNKVRQSPYSIAA
                     DYLDGLEITQQDYNLLMAEHQHNPNTLLVLDPPYVSTAQGAYAADKYFNMVSFLRMIQ
                     YMRPPFILFSSTRSEALDYFQFLQECEPDKYRRFSGYNIVSLDAKMGKGIEYQDNMIY
                     KID"
     gene            26623..27156
                     /locus_tag="WJL14_000250"
     CDS             26623..27156
                     /locus_tag="WJL14_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ7655380.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(27219..28406)
                     /locus_tag="WJL14_000251"
     CDS             complement(27219..28406)
                     /locus_tag="WJL14_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ7655381.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTAPASNNAPSAPSHPVA
                     KARTVYPEPPHHSNSADNLLRIVASSGFAEDINRVNTALTRLYNAGFTVTNQQAGSRR
                     FQRFAGTDAQRAADFQDVASGRVAIPKVLMGLRGGYGAARILPHIDFASLGARMRERG
                     TLFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTV
                     DVPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLN
                     TLYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHI
                     ADKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVAD
                     ISE"
     gene            complement(28446..28850)
                     /locus_tag="WJL14_000252"
     CDS             complement(28446..28850)
                     /locus_tag="WJL14_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ7655382.1"
                     /translation="MTAQPCPICTAQNEDVLLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         7457 a         7628 c         8202 g         6020 t
ORIGIN      
        1 gcgggtttgc aaatcgggct gcaaaaggcc aacgagcaag agccgttcat cgcggcggtg
       61 gccgaaactt taagccgcac gatgtggggc tatgtgatgc ggatggatgc gcggtttgaa
      121 gtaacgttta tcgtagattt gggccatgtg gccttcgagc atcaattcac tgcggtcaaa
      181 caggcgctga aggaaaaatt cgaggttgaa gccgatgggg atgcgctgac tgtggaatcc
      241 gaccgcctgc cctatggtat tgcggcctgc cgggtggtgt tcggagatgt gttatgagcg
      301 gagatacacc gattaccgtg gaatatgtgt tcggcacgtt ggtgtcgttt ctgattgctt
      361 tattgtggta ttgggtaaaa agcatttcag acggcctgaa agaagcccgc aaagatcgtg
      421 atgaactgct cggcaggttg cacagcgtag aaacatctta tcagacgaaa gcggaagcaa
      481 aagacaacag gaacgaaatc ttaaacttgt tgcgtgaaat caaggctgat ttgaaagaag
      541 tcggccagaa aatcgaacgg aaggcagaca aataatgcaa aaccaagacc ctattttaag
      601 agcgttggca gaaatcaacg gcaagcagga caaattgtta caaaatcagg aacgcatgga
      661 tgcggaaatc aagaaaatcc atgccgattg ccgccgcacg tcggcaacca ccggcgcggc
      721 cgcgggcgcg atttcgggcg gtattgtggc tacgggcatc gcatttgccc gcgccaagtt
      781 ggggctgtaa cgatggcgca tccgaaggcg acccgcgaca agctgcgggc actctactgc
      841 aacggcgagc agagccttga gacggcggcg gctttatgcg gcgtatcgct cggcaccgcc
      901 cgccgctggc gcgatgaggc caaggcgcag ggcgacgact ggaacaagct gcgcgcggcc
      961 catacgctgg ccggcggcag catagacgaa atcgcgcggg cgatgatgac gtcgtttctg
     1021 gtgcagtatc aggcgacgat gacgatgttg caggatgcgg aagtggaaga tttgccgccg
     1081 agcaaacggg tgcagctgct ggcgagtttg gccgatgcgt ttaccaaaac ggtggcggcg
     1141 aacaagcggg tgttgccgga aacctcgcaa ctggcgacgg ctttggagtt gttgcagttt
     1201 ttgatggtat tcgtgcaaga aaaacacccc aaacatttgg ctgcctttgt ggaagtgctg
     1261 gagccgttcg gggcggaggt ggagaggaag tttggttagt ttaatcgtga agtaaacgtg
     1321 tcatcagaat gtttaccggc aacaccaatt gcaaccaata gatctagatg atcggctaat
     1381 tttttcagtg aaattttaaa gccttgctcc tccaataaat cttgtatttc agacggcgtg
     1441 tatttgcccg catctaacag tttggatatt tctaaatcaa tcgatttcat tttttgccct
     1501 tatgaaaaat aaagatttcc tcaaatccct gtccgccctc gccgccaatc tgcgccaagt
     1561 catcgaggcc gaagtagacg gtttcgatgc ttcgccgacg gcggtggccg agcggcgggc
     1621 gaaggtattt gacccggtag gcggttacga gtatttcgtc aacacctact tcccgcatta
     1681 tatccgttcg cctgaaaaat ccgacctgca ccgcttctta ttcaggcgtt taccggaaat
     1741 cgtcgaatcc ccggaaggcg agaacgaagc ggttggcgcg ccgcgcggcg agggtaagtc
     1801 gacgcaggtg acgcagttgt ttactttgtg gtgcattgta acgggacgca agcattactg
     1861 cgtgattgtg atggacagca tagaccaggc atacccgatg cttgaggcca tcaaagcgga
     1921 attggagttt aatccccgcc tgaaaaccga ttttgcggat gtctgcggcc aaggccgcgt
     1981 gtggcaggca ggtaccatcg taacggccaa cgacatcaag gtgcaggtag cgggcagcgg
     2041 caaaaagctg cgcggattgc gccacggccc ttaccgcccc gacctgacga ttttggacga
     2101 tattgaaaac gacgaacaag tccgaaatcc cgaacagcgc gacaagctca atgcgtggct
     2161 gacgaaaacc gtgctgccgc tcggtggtgt ggggcaaaaa tacgatgtga tttatatcgg
     2221 cacgattttg cattacgaca gtgtgttgaa ccgaacgctc aataatccgt tttggaaagg
     2281 catcaagttt aaagccatgt tgaaatggcc cgaccgcatg gatttgtggg acagatggga
     2341 agagttgtac cgcaatgaag gtgagttggt ggccgatgcg ttctatcaaa cgaacaaagc
     2401 ggaaatggaa cgcggggcgg aaacttcttg ggcagcgcgc ggcgtgctgg ctttgatgaa
     2461 aatccgcgcc cgcgacggac acgcggcttt cgattcggaa tatcagaatg atccggtgag
     2521 cggtgaagat gcaccgtttg cccaagccat gcagttttgg gcggaactgc ccgccgattt
     2581 ggtgtatttc ggcgcgctcg acccttcgct cggcaaagcg ggggccagcc gcgacccgag
     2641 tgcgattatc gtgggcgggt atcaaagaag cagcggcaag ctgtttatcg tggaagcgca
     2701 aattaagaag cgtttgcccg atttgattat tgaagacgtt atccgcttgc atgcgcaata
     2761 taagtgcaag ctgtggtttg tggaaacggt gcagtttcag gagttcctga aagacgagct
     2821 ggtaaaacga agtgcggcaa aaggcattcc cgtgccggcg cgggcggtca aaccgattgc
     2881 cgacaagctg ctgcggattg agactttgca gccgcacatg gccaacgggt tgattttact
     2941 tcatgccaat cagcaaacct taatccaaca gtttcgccat tttcccaagg cagaccatga
     3001 cgacggcccc gatgcggtgc atatgttgtg gtcgggggca acggccaaca gtgcgccggt
     3061 ggaatatatg gcggtgacga aacatgggac ggacggcggt ttcggcagcg gcgcatggtg
     3121 aaactgacac cgggcagggc ttacgattaa tgcaggcagc agagaattgg ggcaagttta
     3181 acttgccctt tttttattta tgaaaaacat tttcagcagt gcattgagca aaattctgcc
     3241 gggccgctat acaccgaaat ctaccccgca aacggcggaa attacccaaa acacccagac
     3301 tcacgaacac ccgagcaaag gtttgacacc gcagaagctg cacggcattt tggaagctgc
     3361 cgagcgcggc gatatgaagg cgcagtcgga actctttgcc gacatcgagg agaaagacgg
     3421 ccatatcttt tccgagatga gcaagcgcaa gcgggcggtc atcgggctgg attggcgcgt
     3481 gatgccgccg ccgaacagca ccgacgccga acggcggctg gccgaagaag tcaagggatg
     3541 gattgagcgt ctgcccgatt tcgaagacat gatgttcgac cttttagatg cggtcgggca
     3601 cggctttgcc tgtgtggaaa tcgaatggca gcaaataggc ggcctgtggc tgccgaaaaa
     3661 ctttatccac cgtccgcaag gctggtttaa ggtggacggt gccgataatg tgcggttggc
     3721 caaacaggat aatccggatg gggaagagct gtgggcgttc ggctggctgg tacacaaaca
     3781 ccgcagccgt tcgggtttgc tggtacgcgg cgggctgatg cgcacgctgg tttggccgta
     3841 tctgtttaag aattattcgg tgcgcgattt ggccgagttt ctggaaatct acggcctgcc
     3901 gacgcgtttg ggcaaatatg cggtgggggc ggacgaaacc gataaaacca cgctgctgcg
     3961 ggcggtaaag gaaatcggac acaacgccgc gggcattatc cccgaaacca tgaatatcga
     4021 attgcttaat gccgccaacg gcagcagcga gccgtttatg gcgatgatcg actgggcgga
     4081 taaaacatcg tcgaaagcga ttctgggcgg tacgcttacc agcatggccg acggtaaaac
     4141 cagtaccaac gcgctgggtc aggtgcataa cgaggtgcgc catgatttgt tggtgtcgga
     4201 tgccaagcag cttgccggta cgataacgca gcaactgatt ctgcccctgc tgcggctgaa
     4261 taaaggcaac gtggatgaaa cctgcctgcc gcgtttccag tttgatacgc agctacccga
     4321 agatatggcg gtttacgccg aatctttgcc taggctggta gagatgggca tgaagattcc
     4381 gctggcgtgg gcgcaggaaa aactggcgat tccgctggct tcggacgacg agccggtatt
     4441 ggctttgcag acggctgaaa gcgaaggtgt caaagttgcg ccgttaagtt accgccgcgt
     4501 ggccttgagc aggcagggcg aaatcttgga tatggggcag gcggccatcg ataacgcagg
     4561 tttgggcaaa atcgccctgc ccgagcatat tgagccgttt ttgcgcgggt tgggtcaggc
     4621 tttggccgag ggcgacagct atgaagaggt gcaggagcgg ctgttgcgtg cttatccgca
     4681 tttagacagc gccgaatttc agacggcctt ggcgcgggtg attttcatct ccgacctgtg
     4741 ggggcggctc aatggctgat ttgggttttg ccttcggttt ggagccggaa gcggcggtca
     4801 aatattttga aggtttgggc taccatatcc cgcccgactg ggatgtgaaa tggaatgagg
     4861 cgcagaccaa ggcgcggacg attgcaggca tacacaggca ggatattgtc ggcgagtttc
     4921 acgcggcaat gtatgaggcg gcgaaatcgg gcaaatcgtt tgaggcttgg cgcgatgaag
     4981 tgcaaggccg tctgaaagcg catgactggc atctgctcaa agacggcgat attgtggacg
     5041 gagacaccgg cgaagtcctc ggacgcggta ttaccaaaca ccgcatggaa acgattttcc
     5101 gcactcagat gcaatcggct tacatggccg gtcattggca ggcgtttgaa gaaggtcggg
     5161 atgatgcgcc gtggctgcaa tattcggcca ttttggacag ccgcacccgc caaagccatg
     5221 cggcggcgca tggtgcggtg tatcacatcg acgacccgtt ttgggattac ttctacccgc
     5281 ccaacggctt caattgccgc tgtaccgtgc gggcgctttc agacagtgat ttgaaacggc
     5341 gcaatctgct gccgcaaaaa gcgcagcttg aagatacgga agtggtggtc aaccgcaagg
     5401 gcgatacccg ccccgccaaa gcggtaaagc tgccggacgg ccgccgcttt tataccgatg
     5461 cgggctttca gcacaatgtc ggtaaaagcc atttggccaa cttggggcag ttgcagatgc
     5521 agcgtgcagt ggaactgccg ccgaaactgg cgagcgtatc gattcagacg gccttaaaac
     5581 agcctgattt gatgcgggcg gtatcgcaac aggcggcgca aatggtacgg cgggtggatg
     5641 cggaaaaagt agcgcgcggg aaaacgctgt atgtgggcgc attgaccctg cccgtgttgg
     5701 acgcattggc ggcaaggaaa atttacccgc aatccgccgt gattgccata agcgacgagc
     5761 gggtattgca cgctttgcgc gacaagaagg tcaagccgct gccggtatcg ttttgggaga
     5821 agatacccga actactgcaa gagccggagc agattttatt gggcaaggcg ggacgaaatg
     5881 acgatgccaa acagtttttg gcatttgtgt atccgctgtc ggcaggcaag gggaagctgg
     5941 tggtaacgct ggactatgat gtgaaaaccc gacatccgtt gacaggtaag aaagaacatc
     6001 tgaccttgaa tatggtcaac accggcatga ttgcagaaac ggataaacag gtggatagct
     6061 tactgtacgg gtatgaaaca atatggaaaa agccataaaa ctctttgcct gattcgaaca
     6121 ggataatacg cgacacggta acgtggccgt aacctttcca gtaggaaacc cgagttttat
     6181 ggctgttgga accaatatac catgattgag ataaaaatca acacagacac cctgcaaaac
     6241 agcttaaacg ctgcggcgcg gcatacctcc cacaccaagc ctttgatgac gcggcttgcc
     6301 cgcatcatgc gcaacgccgt actggagaat ttcgccgccg gaggccgtcc cgcttgggcg
     6361 ccgcgcaaat atccgactgc gcgtgaaggt tcggggctgt tgcaagccag cggccgcctg
     6421 cgcaattcga ttacgccgag cagtaccaac gatacggcgg tggtcggcac caatgtggaa
     6481 tatgcggcta tccataactt cggtgggaaa acctcgccac acttgattag accgaaaaaa
     6541 ggcaaagcgt tgaaattcgg cggccgcttt gccaagcagg ttaaccaccc cggcagcaat
     6601 attcccgcac gcccgtttat gaccctgcaa ccggaggacg aaaaggccct atccgatgcg
     6661 gtggcggaat atctggcaca ggccattcag gggcggtaat catgcctgcc tgaaaaatgc
     6721 cgaaaaacag cccgaaaacg ccctaaccca tacttacccc tacccatcgc ctgaaaatca
     6781 atcctgcgcg cgtttgaaca ccttttgaac actatcccac gcgtatctct gcaagtacat
     6841 ttcccttccc tagaaattca tctgtctgac ataggccaag ctttgacgag ggcgcggctg
     6901 ccgcacaatg cgggctatgg acaccaaaac ccttcttgcc gccttatccg ccgcccatgt
     6961 cggcggttcg gacggcttaa tcaaaatcgt acccaaaggc caattcgcac cggttgacgg
     7021 ccgcaccgat acgggtgtgc cgcactggac gatgtctgcc gatttggcac agcaaatcat
     7081 tgccgccttt gacgccgcgc aaaccgacct tgtggtcgac tacgaacacg ccacgctgaa
     7141 agccgccgaa acggggcagc aaaaccctgc cgccggctgg atcagcaaat atgtatggga
     7201 tgacgagcgc ggcctgatgg gcgaagtgaa atggacgcaa cgcgccaaag acatgataga
     7261 cagcggcgaa taccgctatc tgtcgccggt actcgaatac gacacgctgg gcaatgtacg
     7321 cgggctgcac agtgtggcgt tgaccaattc gcccgcactg gacggcatgg ctctggctgc
     7381 attgagccgc caaaactcta tcaaccccaa acaggaaaca agtatgaaca aggaagcttt
     7441 aatcaagctc ttgggcttgg cggcggatgc cgacgacaaa gccatcgaag cggctttggc
     7501 cgaagcacag gaaaagctgg gcggtaaaac gcttgccgaa gcactggccg cacccaaaga
     7561 agaaccgcaa ggtagcgaag gcgataaagg cgatgccggc aaacccgaag acaagccgca
     7621 aggcggcaat gccgacgacg gcgaggtagc cgaactcaaa gcgcaagtgg ccgcgttgag
     7681 caaaaaagtg attgcaatgg aagtgggcgg tacttcagac ggcctgatcc gtgccgcgct
     7741 ttcagacggc cgcctgttgc cgcatcaaga agcatcggcg cgccaactgg ccgccaaaga
     7801 ccctgaagcg tttaaagccc tgattgacgg cagcttggca ctggccgctt tgagcaaaac
     7861 gcagaccggc ggcaaaggcg gcgcagacgg tacgtccgcg ctgaccgccg aagaagcagc
     7921 cgtcgccgcg caattgggca tctctgccga agattatgcg aaggccaagt aatccgataa
     7981 aggaaaagac aacatgatta tcacaccaga caccttaaaa gccctgttca ccggctttaa
     8041 aaagaatttc caagacggcc tgaaaatggc ggaaagccaa tacaaggaaa tcgccaccgt
     8101 cattccgtct tccactgcgt ccaatactta cggctggctc ggccaatggc ccgctttccg
     8161 cgaatgggtg ggcgaccgcg tattccaaga tatgaaggcg cacggctatg ccatcaccaa
     8221 caagcatttt gaaagctcgg tcaaggtcaa ccgcaacgac atcgaagacg acaacgtcgg
     8281 catttacgcg ccgatgatga ccgagatggg ccgtgcttcg gcggtgcatc ccgacgaatt
     8341 ggtgtttgcc ttgctgaaaa acgcacacgc cacgttgtgt tacgacggtc agaacttctt
     8401 tgataccgac catcccgtgt atgaaaaagt ggacggcacc ggtcaagcca gtaccgtatc
     8461 caatattttc gccggcagcg aagcggcctg gtatctgctg gacactaccc gcgccctgaa
     8521 accgttgatt taccaagagc gtaaagcgaa gcagtttacc gccatgaccg ccgataccga
     8581 cgaaggcgta ttcatgcgca acgaataccg ctacggcgtg gacggccgtt gcaacgtggg
     8641 cttgggcttc tggcagatgg cggcgaaatc gcaagagaaa ctggacgctg ccggtttcga
     8701 gaaagcctac aacgcgatgg tgagcctgaa aggcgacggc ggcagaccgc tggccatccg
     8761 cccgaatgtg ctgctggtac cgcctgcttt ggaaaatgcg gccaaagagc tggtggaagg
     8821 cgaccgcctg gccaacggcg cgtacaaccc gaacaaaggc aaggcgaaag tcatcgtatc
     8881 gccttggttg ctgtaacgaa caggcggaca ccgcccgccg gaagggatag aaaatggcaa
     8941 aagaaaaaaa cggacaggaa gttggcgcga ccgtcgatgt ccaaccggaa gaggtaaccg
     9001 taaccgctgc cttacaggcc gagattgaag ccttgagagc cgaattagac aaagccaatg
     9061 ccgaaattca ggtagcgcag gcagagttgg ccgccgcgca agagcgcaat gccaaattgg
     9121 aaagactgct tgaagccggc cttgcacccg gcggcatttc agacggcgaa actttgacac
     9181 ctgaagcgga agccgccttt tacaccggca gcggagcacc cgccgcagat gccgaagtgg
     9241 tggcgattaa gagcaaacac ggccatgcgt ttttccgcgc cggctaccat gtacagccga
     9301 actggacgtt tgtgcgccgt gccgacttcg aacccgccga ctttgagcgc ctgattggcg
     9361 accgtatggt tgaagcgcgc gaagccctgc cgacggacgc atcatgagct acgccgccgt
     9421 tgccgatttg gtggcgcgtt tcggcgaagc caccatcacc ggcctgaccg acttgtcccg
     9481 caagggcgcg gtggacgaaa ccgtcgcaca gcaggctttg gatgatgccg ccgccgaaat
     9541 cgacggctat ctgatgaacc gctataccct gcctctgccc aagccgctgc gcatcttaac
     9601 cgtgtattgc tgcgatattg cggtgtaccg cctgtgtacc ggcaaacggc agcttaccga
     9661 agacattgtg caccgctatg aagcggcagt gaaattcctg caactggtgg cagcggggaa
     9721 agtcggcttg ggcgttaccg aacccgcagg cgaaaagccc gccgtacaag gcaacggcgt
     9781 gatgtttacc acacaggaaa aggtgttcgg tcgtgatagc gtctattgaa caagccatca
     9841 ggcagcggct ttcagacggc ctcggccaaa tggtaacggg tgtctttact tacggcggcg
     9901 agttcgacgg cgaaggtttg gcgcaggtgg tcaaccagtt ccccgccgtg tgggtcatgt
     9961 ttgccggcat caaagacacc atccgccacg atacgcgcgg cagccgtttt aaagcgattg
    10021 gccaattcac ggtattggtg ggagaccgtg ccagcggcag cgaagcagac agccgtttcg
    10081 gcggcctgca ccgccatgat gtcggcacct accgcctgat gcagacctgc cgccacctgc
    10141 tgaccaatca atcgctgggc ttacagatag accgtctgca accgggggcg gccaaaagcc
    10201 tgttcagccg ccaaatggag caagacgccg ttagtgtgtt tgcgctggaa tttgaaacgt
    10261 attggtttga agacgcgctg caagacggcg attggccgcg gcctgcggtt tcagacggcc
    10321 aacaggcgaa agtatatgcc gacgtatccg aataccaagg ccgcaccgaa cccgaacatc
    10381 ccgactttaa aggcgtgaac cttgaattgc gtatcccgcc gaaaaccccc gaccaacccg
    10441 ccgatatggc ggccaccgtt gaaaccaagg tgaaaccatg aacgaaacca ttaaagtacg
    10501 cgccgccgac ggcctgcaag tgccgctggc cggtaaacca catgaataca ttaccgacca
    10561 agaaaccgtt accgtacccg atgccgccta ttaccgccgc tgtatcgaat acggcgacct
    10621 tgtggtcgta accgaagaca cccaacccgc gaaaggcagc aaataatggc atccgcaaac
    10681 atcaatttcg aaaaaatccc cgccagcacc cgcaagccgg gcgtgtacgc cgagtggaac
    10741 accaagctgg ccgtgcgcaa cctgcccacc aacaaacagc gcgtgttgat tgtggcgcag
    10801 cacaacaatc ccgctttggg tgagcttacc gagctggaaa acgtgttttc cgccgccgat
    10861 gcggccgcca aatacggcgc aggcagcatg gcgcacctaa tggttaccgc cgcgattaaa
    10921 gcctatgcct atgccgattt aagcctgatt accgtagccg acaacaaggc cggagtggcc
    10981 gccggcggta aaatcacctt aagcggtact gccaataccc aaggcgtgtt gcgtgtgagc
    11041 attgccaatg ctgacacttt aaccatcggc atcggcgccg aagacaccgc agccaccgtt
    11101 gccgccgccg tcaaagccgc catcgatgcc gtgcccgatt tgccggttac cgccaccgtt
    11161 gccgaagcag tggtgacgct taccgccaaa aacaaaggta cggccggcaa cgccatccgc
    11221 atcaaaacca gcaataccgc cgaaggcatt accgccgccg ttaccgccat gacgggcggc
    11281 gatgccaacc ccgatattgc cgccgcgctc aatgccgtag tcgccgaagg ccaccacatc
    11341 atcgcctgcg gtatcaacga cgaaaccaac ctgctgaaac tgcgtgccca tttggatacc
    11401 gtagccagcc cgatggaaaa acgctgggca atatgcgtat acggccaaac cggtacgctg
    11461 gcgcaagcca ccaccttggc aggccgtctg aaccacggcc atatcgtcag cgcgtggtat
    11521 cgcggcaccc ccagcctgcc ttgcgagctg gtcgccgcct ttgccgctgt gatggcgagc
    11581 gaagaagacc ccgcccgccc gttgaacacg ctggcattaa acagcatcgg cgtgtgcgaa
    11641 agcaaagaca aaaccatgcg caccgagcag gaaaacgccc tttataacgg tgttaccccg
    11701 attgaaacca gtccggccgg tacgcaggcg caaatcgtgc gcgccatcac cacctacacc
    11761 aaaaccgcca acggcacggc agacgaaagc ctgctggacg taaccaccgt gcgcaccctg
    11821 atttatgtaa gccgcgcctg cgtcgaccgc atcgccctaa gattcccgcg cgacaaattg
    11881 agcgaccgca ccccgccgcg cgtgcgcagc gaactgattg acgtattgat gtgctgcgaa
    11941 gagctggaaa ttttggagcg cgtggaagaa aacctgccga aactgattgt ggaacgcgac
    12001 ctgcaaaaca ccggcatgtt gaactgccgc attcccagcg atgtcgtcaa cggcctgcat
    12061 gtagtaggta tggttgtaga tttatatttg taagaaaggc tgaaacatga gtaccgaata
    12121 cgttggcagc gtcatcctct atctgggcgc gaacgaagtc caagtaacca aaatcgacgt
    12181 gaaagacaac accggcaaaa agcccgtaaa aaccatgaac cgcacccgcc gcgtcaaagg
    12241 ctttacccgc ggcgtcggcc aatacgacat tacctttacc gccgtggtgc cgaccgacgg
    12301 cacggcggtg gactgggcaa agatcgacga cgccaaaatc agcctagttc ccgacgtgga
    12361 aggcgcgcgc ccgacatcgt acctcggctt ctgcgccacc gaagcgggtg acagctatac
    12421 cgtagataat gagctggtgg tggatgtgac cggttttgcg attcggaagg tgttggagta
    12481 aagattttct tgttaacaga agccgctgaa gcggcttctg ttgtttttat tacactgttt
    12541 ttaataaaat tcaatttttc aagtaaaata cttgaatttt aaaaaagcct ttgatattat
    12601 ttagccacgt tgaatgtggc agtaatgctg caaacacatg aaaaggagct tgaacaatgg
    12661 gactgactaa attcggcgca gccgttcgtg aggctcgtcg tcaaactaaa caaactctgc
    12721 aaacgatggc tgctacatta ggtacttctc ctgctttttt gagtgctatt gaaactggtc
    12781 gtagtaaagt gccaatggat tttgttaaaa aggttgaaga tttttttgaa aacttaggcc
    12841 aaccaataga cggtttaaag caaaaagcga tggtttctaa tgagaatgta tcgttaagcg
    12901 gcttaagcct gcaacaacaa atgctggtgg ctggttttgc cagttcagac ttcagcaaag
    12961 agcaattgga caaatttgct gaattattaa gaaccattca tcataaagat tcacagaaag
    13021 aaggggatga tgcagcaaac taattattca atgcgtggtg tgcgtgtggg aatgttaagc
    13081 gaagaaacga ttcagttttt tgcaaacgaa gctgccaaat ttttggtagt agataagcgt
    13141 actcgtaaaa aaatggatgc atttatggaa atgctggaag agtatggcat tgtaattgat
    13201 gttgttgctg attctgaatg gctagtcatt accaacgcaa tgtgccataa cggaacgatt
    13261 ctgatgccta atagcctcta tactcgcatt tgcaatggcg aggacgaggc tatctttatt
    13321 ttctttcatg aattggggca tttgctttta gggcataaag ccatgttgca ttatagtgaa
    13381 gttcttccca cgaaacagga agattcagaa tggcaagctg atgagtttgc aaaatgtatt
    13441 ttaagaaaca tgggaattaa atatgtacca gaacagttaa gcttacgatt ttaaaaagag
    13501 aaatgacttg gaatgttggc gcatcccaag ccattattgt ggtgtactag catacttcca
    13561 atatgctgta caagtgtagt tctccatccg ctaactgcaa acagatttca aagagaaata
    13621 cttgaaaggg acttgagtat atatttttca gatttgtttg tccacgaaaa gcgaagtgat
    13681 ggccaccatt tttacaattg gaggtgccat tatggctatg actaaggctc ccgttacccc
    13741 cgacttggtg gacgatacag gtgcatacat ttttagaaag tcttttacca ccaaaagtgg
    13801 taagaaaatt gtttctaaaa atgggaagcc atttaaaatt cctgtgaaag tttaggaata
    13861 tataagcccc gctaagcggg gttttttcat tactgacgcg tgtttagtcg cccatctcat
    13921 ctccttaagt gacaattaag ccttgattta tacaaggttt aaccaacgtt taagggcttt
    13981 tttatgtctg aattttcccc cgaactcacc cgcgctatcg aagattacga attacgcgtt
    14041 tctcccgacc tgaaaaccgt tgccggccgt ctgaaatacg gcatcggtgt gattgatggc
    14101 gaaatgcacc atgatttttc catgcacctt ttaaccgtgc gcgaggacat ggcgattgat
    14161 ccgcaacttg aaggccagcc gcggctggtt gccgcctatg ccgcgtcact ggataaattg
    14221 ggtggtttaa cagccgaatc tttgacacct gatttgctgc ttgatgagat ggctgctgct
    14281 gactttgacg cgctgtactg ggcgcaagag ctgctgcaaa aaaagcggct ttgcccgcac
    14341 cccgcgccga ccgttaccga tacgccgtct taaaactcgg ccgatacggc atcacgcccg
    14401 cccaaatcga cggcatgacc caacccgaac tggaaggctg gttaaaacag gccgacttaa
    14461 tcgagcgcgg ccgtgccgcg ccggtagtga tgccgtggtt tattcctgcg aacgtcaaac
    14521 catctgccgc cacgggcggt cagacccaaa cctttatcag caagcgtaag aaaaaatgag
    14581 ccagcatagt gtagaactgg ttgctaagtt taaggacaac gccacgcccg gcctccgccg
    14641 tttggcggcg gaagcggaaa aaacaggcag ccggcagttg cagaaagccg cagcagtcaa
    14701 gctgaaacat caagagatgt acagcgctac cgcccgtttg ggcatccgta ctgaacacca
    14761 aatccgccgt gaaatccaac aaacgcaggc ggcttacaac cgtttggcca aaagcggcat
    14821 ggcttcgcaa cgcgaactgg cgagggctgc acagataacg cgcagccgtg tacgcgagct
    14881 gactgccgaa atcaacggcg gtgcaagccg tttgcaacgt atcggttcgg gagtccggac
    14941 aatcgggcgc ggtgtggccg gcgtggcggc gggtgcggcg gcaggagctt atgtattggc
    15001 gcagccggtc aaccggacga tggactttga cacatcattg cgtcatgccg ccaataccat
    15061 gtatgcaggc aagagcatgg ccgagaagcg cgcaggtatg gaggaaatca agaaatcggt
    15121 catggatgcc gcctatgtcg gcggaacgac gcgtgatgcc gctttggaag ccatgaatac
    15181 aatggtggcc agcggagcga tgagcgacga agccgtgaaa aaactgctgc cgaccgttat
    15241 gaaaaccgcc accgccgcca atacggaagg aaacgatatt gccaacatcg taaccaaggc
    15301 gttgcaggcg gggtttaaag aggcggatat tccgtcgctg cttgaccgtg cgttgcaatc
    15361 gggcgcggac ggcggttttg aattgaaaga catgtcgcgc tggctgccgc agcagttggc
    15421 tgcaatgaaa aacgccggta tgggcgcgac gctggataat ttttccagcc tgctcaatgc
    15481 caaccaattg tcgtttatga cggcgggcag taccgacgaa gccggcaaca acttagtcaa
    15541 cctgctggca aaaatcaaca gtcaggatat tgtgaccaaa gccaaaaaaa ttaccatcaa
    15601 cggcaaagaa ggtttcgatt tcaccgctag catgaacaaa cggcaggcag caggcatgaa
    15661 ctcgcttgat gcgctggtgg acattgtcgg agaaatcacg gccaaggata agaagtccgc
    15721 cgctctgatg aaacagatta atgccgcgca aggcgatgaa gccaagctgg ctttgctgga
    15781 aaaccaaaag gccttggtag acggcactgc catcgggaag ctggtttccg accgtcaggc
    15841 attgatggca ttgctggcat tggtcaacaa caaacaggaa ctcacccgcc tgcaagcagg
    15901 ccagacaaat gcggcgggtg cggtggacgg tgcatacgga ttcgtcgccg aaggttcggg
    15961 gtttaaaaaa tcccaattct ccctcgccaa atcagaagcc gaatacggcg catttgaaaa
    16021 attcagcgac cgcatcggcg gcggccggta caagctctat gtttgggggc ggcggcctgc
    16081 tttccaaact gttcggcgga agcagcagaa ccttacaggc gggccgtctg atgccctcgg
    16141 cctcctcgct aggccccgtc gcgctcggtg cgacaccgtt ggccgtaatg ggcggggcaa
    16201 cccatttggc ggggcagcgc gacaaatatg cggaatggtc aaaaccgttt gctaaattat
    16261 cgtcttggct ggaaagtatc ctgcctgatt tttcagcggg ggcgaaagcc gaatatctgc
    16321 gcaaacgtga agaactgggc ggcaataatg ccccgctcga tagtcctgtt ttaaaagaga
    16381 gtatggcgca gcttaaccaa tcggcacaaa ccaaccagca ggcaagccag cagtatgtgc
    16441 aggcggcggc ggagaatcag gcggcaacgg cgcagattac cggcgcggcg gcacagatga
    16501 cggctgctgc cgcacaaatg caggcggcgg caggcaagcc gattccgatt accgttactg
    16561 tgcaaaacgg caacattatg gcctatgtga atcaggcagt agaacgcaac agtaggaaaa
    16621 attaatggct tggaaagaca ctttactgga tgcgggcttc aagggcgtta cctttgacgt
    16681 catcgacgac acgctgcgcg gcacccacgc gctggcggaa cacgaatacc cgttcgtaca
    16741 gggcgcggac atcgaagata cgggtgtatc tgcgatggat atggccttga ccgccgtatt
    16801 gtggggtgaa gattacgaag gccgcctgca aaacctgctg aacgtattgt gcgaaacggg
    16861 cgcgggcgaa ttgatccacc cgatttacgg cagcgtgcca gattgcgtag tggcggattt
    16921 cgaggtcgcg cacaacgaag aaaaccccga ctactgcacc gtgcggatga cctttaagca
    16981 aagtgtcaaa gccgcgccgt ttttcgaccg cgacttgccg atagccttgg ccgacgaagt
    17041 ggatttcctt gccgacttgg ccgcatggca gggcttcgag gtgtttcaga cggccttaaa
    17101 caaaattcag aaagcgcaaa gccgctggaa tgcttttcat gccacggtat taatggtggt
    17161 gggcgtattg tatgggcagg taaacggcat cttcaccggc ggcctgaacc tgctgaacag
    17221 cccgcgcgta ttggtggcgg agctgaaatc tgtgtttggt gcgctggcca atatgcacaa
    17281 tgtcggtaaa agcgggctgg acggctggcg cgacatggtg ggcggcgtat ctaaagcggc
    17341 cgctacgccg tggcaggtca gccgcggaac cgaaggcagt gtggcggcgg tagatttaat
    17401 ccaacgcgcc aagcccgaag atgtggctgc ctttaccgcg cttaccgcca ccgttggcgc
    17461 gtgtgcgctg gccgaacaag ccgccgatat tttggccgta caactgaacg agccgacttt
    17521 gacgcctgtg gaaatctcgc gtctgctggc cgatacccgt gccgccttgc agcgtgcttt
    17581 ggccgcccag cgcatcttgg ccatgatgct ggcggatgaa accaaagccg acaagctggc
    17641 ctattgcctg ctgcggctgt atcaaacacc ggtagacagt gccgatgatg tgtaccaacg
    17701 catcgaagtg gccgggctgc tgccgcaagc cccttatctg gaaactgccg ccgcgcttac
    17761 cgaaagtctg cgcaatacgg cgcataaact gcaaaaacag gcgtttacag tcatcaatat
    17821 gaagccgccg ctggtacaga aaaccgtcac ctgcgatatc ggcctgcacc tgctggcctt
    17881 tgagtggtac ggcgactaca gccgctttgg cgaactcttg cgcctgaatc cgcaaatccg
    17941 ccacccgaat tttattgaga aaggcacggt actgaatgcc tacgccagat aacaccgtta
    18001 cgctgctgat taacggtaaa acacacggcc aatggacgaa ttacgacatt gtttccgacc
    18061 tgctcacccc cgccgatgac ttttcagtca tgctcggccg ccccgtcgat gccaaacccg
    18121 atgcggtgcg ggcgggcgac aaggtggaag tgcgtgtggg cggcgatacg gtattgagcg
    18181 gccgcataga ccgcgtgcag accgttaccg aaaaaggcgg caaaacctta accatacaag
    18241 gccgcgacga tgcgggcgtg ctattggact gctccgcccc gctgtttaac gcgcaagata
    18301 tggatttaaa ccaaatcatc gaaaaaatcg tcaagccttt gggtttggcc aaaatccgca
    18361 ttgatgccgc caaaaccgac aaaacccaca aagtgcagat cgagccgggc agccgcgcgt
    18421 gggacgccct gctggaatat gccgaggcaa acggtttgtg gccgtggctg gagccggacg
    18481 gcacgctggt agtcggcggt cccgattaca ccgccgcacc ggtggccgaa ctggttttac
    18541 ggaccaacgg ccaaaacaac aacatcaagc gtctggaagt aaaccgcgat atggcggcac
    18601 ggtacagcga agttaccgta ttggcgcaaa gccacagcgg caaaaacaac atcaaagcca
    18661 ccgccaaaga cgaatccgtc aaactgcacc gccccctaat cgtcaccgag ccggacatcg
    18721 acagtcaggc tcaagcgcag cgcaaggcga aaaagcggct ggccgacagc aggctggaag
    18781 gcttaacaat taccgccacc gtacaaggcc accgcactga cgacggtacc ttgtggcagc
    18841 ccggtcagcg catcaacgta ttgagcgaac cggacggcat cgacgcggtg tatttcctga
    18901 tggcgagaac cttcatcggc ggtcgcggcc aacccaccga aaccgtgctg acactgaaag
    18961 aggacggcgc atgggtattg gatgccgacc cgccgaagaa atcgggcaaa accaaaaggc
    19021 cgtctgaaag ccgcaaagcc aacggccacg cagccgccaa accgaaaaaa cgccgccaag
    19081 ccaagcaggc cggacaggaa ttgcaggtga tttaaatgga tgcaaaaacc atagacaacc
    19141 gtatccgacg ggcatttaac ggcatccgcc aagccttccg cggcaaaatc gcccgcgtca
    19201 aagcagccgg cggcgtgcag caaatccaag tggaaggttt ggaaggcgaa accgtgcagg
    19261 acttggaaca cgccgaaaac ttcggtttta cgtcgaaccc gcccgcaggc agcgattgcg
    19321 tcgtcgtacc gttgggcggc aaaaccagcc acggcatcat cgtcaccacc accaacggcg
    19381 cataccgcat taccggcctt gccgaaggcg aaacggcggt ttacaacgcc gacggtgcca
    19441 agatggtgtt aaagaaaggc cggattatcg aaattgactg cgaaacgctg aatatcaaag
    19501 caccgggcgg cgtgaatatt gacgcgccca atgtaggctg caccgcgcag attaccgccg
    19561 agggtcaaat caacggcaac ggcggcatgg cagtgaaagg cggcagcggc acatcattta
    19621 ccggcaatgt gcagatggtc ggcgatttgg acactaccgg caaactgacc aacaacggca
    19681 aaaatgtcgg ctccgaccac aaacacaacg gcgacagcgg cggcacgaca tccgacccgc
    19741 tgtaattcag acggcctgac atccatcaaa ccaaactcca agcgtcctta cctgaaaata
    19801 aaggtatgga cgctttactt aatccccaaa cgggcggcta tgtggtcaac caatccgccc
    19861 aatccatcga aaacgagttg tatatccgct tggtcacgcc cttgggcagt tactgggcag
    19921 accgcacgct cggcagccgc ctgcacgaat tgcgccgcca aaagcattta aagcgcatcg
    19981 aagtactggc caaacagtac gccgagcaag ccttgcagcc cgtgattcag tccaaacgcg
    20041 cccaatccat ccaagtaacc gcatccgccc cgcagcacgg ctggctgaag ctgcatattg
    20101 aagccgtcga tgccgcgggc gatacagtaa ccctgaacca caaggtagcc gtgatatgac
    20161 gcaggcactg aatttagaac aaatccgcgc caactatctg cgcgacctgc aaaaccaaaa
    20221 ccctgccgcc catgtacacg cgggcagcga caaccatgta cgcgccaccg ccattgccgc
    20281 agtgggcgaa ggccaatacc agcatcagga gtggattttg cgccaagcct ttgccgatac
    20341 cgcagacagc gcctacctag aaaaacatgc cgctaaatac ggcatctacc gaaaaaccgc
    20401 caccttcgcg ggcggcaagg tgcgtgttcg cggcgcggtc ggcgcaacgg tgccggttgg
    20461 ccaacagatc aatgtgggcg ataaggtgta tttaaccgcc gaatccgccg ttatcagtgc
    20521 gcttggaagt gccgaaatag ccgttatcgc cactgttgcg ggtagtgctc aaaatcaaac
    20581 cgccgaaacc gcggctacgc tgcaaagcgt acctgcgggg attgacagtt ccgccgtcta
    20641 acgatggtcg gcggtacaga tgccgaaagc gatgagagcc tgttggcacg atatgaagaa
    20701 cgtctgcgcc gacccgctgc gggcggtaat caatacgact tccgcaattg gtgcttggaa
    20761 gtaccgggtg tggttgatgc ctttatctac cctttgcgtc gcggcaacgg ctttgtcgat
    20821 gccgtcattt tgggtgaaaa cggtatcccc agcgcagaaa cactggccgc cgtacaagct
    20881 catgtcgatg cggtgcgacc tgttacccgt aaaaacggct ttctagccct tgcgcccagt
    20941 atccagaccg tgaatgtggc cgtcaccatt accttaagca gcggcacgga taccgatacg
    21001 gccaccgctg ccatcaaatc agctgtaaac gcctattttg atgccttaaa gcccggagac
    21061 cccctaatta aaagccagtt ggaaacctta atcagcgaag tgtacggcgt gcgtgaccgt
    21121 gtcttaacca cccctgtggg caatatcaag cctcaggaga gtgccgaaga catttactgg
    21181 ctgcgcccgg gcagtattaa cgtggagtac accacgtgat ccatcaaacc ttactcgccg
    21241 ccatgcgtcc gcccgtcagc tacgacaccg taggagaaac ggcagaaatc aaagccgaag
    21301 cgggtgtgtt tgatattgtg gccgaccatg cggaaggagt gaaaaatgcg ccgttccctg
    21361 atgcggaaaa cgattacctg taccgctggg aagagctgct cgccatcacc ccgcccgcag
    21421 gagccaatac ccaacaacgt actgatgccg tgctggccaa actcaatgcc ttgggcggtt
    21481 tgagtattgc ctactttacc gccatcgccg aatcggcagg ctacaccgtc accatttacg
    21541 aagaagacca attccgcgca ggtgaaagct gtgcgggtga ttgtttgaat accgaagacg
    21601 ccatctggcg ttggtgcgtc gacatcgccg acggcaaagc caccgcctat attttccgag
    21661 ccggtcaaag ccgtgcgggc gaccgcatca gtgtgtacac cgacccgatt atcgaaacga
    21721 tgtttgaaga attaaaaccg gcatggacgt attgccgctt tgaatatgaa gaagaggtat
    21781 aaaaatggac ttaatccaaa ccccgaataa gcaatttgtc gacggcgacc gccgcacgcc
    21841 cggtactccc gtacccgcat ggtggctgaa ccagttacaa ggcgagttgt acagcatttt
    21901 aaacgcggtt ggcattgagc ctaacaaagc cgaccatgcc caagtcttat cggccattaa
    21961 aacgttggcc gccgatgctt cgcaggttgc cagtatcgat gctctgcgta aatacagcgg
    22021 cacaggctat gtgaacgtca acgcctatca cgccaataca acagtgggcg gcggcgtgtt
    22081 tgtggcggat aaagccgata aatctaccgc tgataacggc tgtaccgtta ttgtttctac
    22141 cgacggcacg cgctggaagc gtgtgttttc agggatgctt aacctgcatg attttggata
    22201 tgtggccagc aaaaacaatg cactatccac tttgaatgcc gctgaatctg ccgcgcttga
    22261 cgtagttgtt gattgcttgg gtttgtcaat tgatacgggt aatacctacc cgcaaaaaaa
    22321 caaatacaca aacggcaagt ttgtgattaa cggcaaaact gtcgatgttc aataccagcc
    22381 tatcagaagc ggtatcggtc gattcatctc cggaactggt gcagcagcca acctcaaatc
    22441 gaatgaatgg actggcgcgg gtttaatcgt tattggcgaa ggcgcaatgg agcagatgga
    22501 gaaatgtgtt tcctcaatcg ctattggcga ccgtgcgcag ggcttttcta aagtaagcag
    22561 ggacaacatc gccattgggg ccgacagcct gattaatgtg caggccgcta ctgaatggta
    22621 cgaccagtca cgcatggaag gcacgcgcaa catcggtatt ggtggtaatg caggacgcgg
    22681 catcaccagc ggttactcta atgtgtcaat cgggcgcaat gccggacagg gattgggtga
    22741 aggctcgtca aatattgcac ttggcgcagg cgcgatggct ggtactgctc cagtcggttt
    22801 tagtggcgac attgaagttt tttggccgtc ttcgacctca agaacaatcg caatcggcga
    22861 ggctgtcttg caaacatatc agggccgcgc cgctcaaacc gcaattggtg ccaatgcggc
    22921 gcgaaataca aaaaaggccg aaaaagttac cgcaatcggt tctgccgcga tggagaatct
    22981 tgagcgaaac cgcgccccaa atggcggaga tgttgtctgg acgggaacgg aagcaggtac
    23041 ctacgcccaa tctggaaaaa acatcacgct tacatttccc aacattcgcg gtgcgcaagc
    23101 gacttattgg gtgggcatcc gccttacatc aggcacggcg caaaccttac aaaacgacgt
    23161 cgtaccggct caggtcgtat cagtgaatgg caatacatta atcatccaaa gctcaaaaga
    23221 gctgaccgcc accggcgcgg ccgaactgaa atacgtttat tctgtaaatt caaccgctac
    23281 taaaaacgaa gagttgacca tcatcggcgc gaacgccatg aataaggcat tgaccgcagg
    23341 atactcaact atcatcggcg tagatgccgc gttgttggga gacaattatc aaaaaacaac
    23401 cgcaatcggc gcatcatctt tacgaacagg tagtcatatt tccacaactg ctattgggta
    23461 ttgggtaatc cctttggcaa gtagtgagaa atgtgttgcc attggagata gtgcgggcta
    23521 tcggaacgtt caaggcgact ttttgactgg gaaaataaca aactccatcg ccatcggata
    23581 tggcgcaaga ataaacggcg ataacgaaat ccaaatcggt acgacagggc aaactttata
    23641 tgctccaacc gcggtgaaca tccgttctga cggccgcgac aaagcagatg ttaagccgtt
    23701 gacgaacggt ttagattttg taatgaagct caagccgatg actggctact acgaccgccg
    23761 ggattcctac gttgacgaat tattcaaaga cttgccggca gatgaacgag cggacaaagt
    23821 ccgcgaatgg tgggcgaatc caatcaagga cggcagtcat aaagaagatc ggttgcggca
    23881 ttggtttatt gcccaggaca ttgctgcgct ggaagatgaa tatggtcgat tgccgatggt
    23941 aaataaaaca aacgatacct acaccgtcga atacgaaacg ttcatccccg ttttgactaa
    24001 agccattcag gaaatggccg caagaattga aacattagaa accgaaatga aggaatcgaa
    24061 aaaatgacaa gatgtgtgat tgatcaagac ggcttgttcg tagaggaaca gtattttgat
    24121 gacggccgcc aaagcatcga agctgaaatg cctgatctcg cacaatatca ggccgcccag
    24181 tgggatggac aaggttggaa gataattccc gactatcgcg gatgcgtagt ttttgccggt
    24241 gggcaagagc aggtgtggga taagttaggt gatttgcccg atggcgtcag cctgaccccg
    24301 cctgaatcgg taaatattga cggcttaaaa tccgtaaaac tcgtcgcatt aaatgctgcc
    24361 gctcaggctt ttattaacaa gcacgccggt atcgacagcg tacctgaatt tgagtttgca
    24421 agctgggcaa ttcaagccgc tgaagcgaag gcttggcagg aagataaagc cgcgccaacg
    24481 ccagtgcttg acggcattgc caccgcccgc ggtatgtcag cagacacgct taaagcagcg
    24541 gctttgcgta aaacgctggc ttacgaacaa ctcgccgcac atgtggcagg tcaacgacaa
    24601 gcgctgcaaa gcaaaatcga agcagcgaaa acgcaggccg cgcttgataa gattgcagtc
    24661 gtattcacac tgccggaggc cgtctgaatg gttcaagtct atttggcact ctataaaggc
    24721 aaagccgcaa tcaacacccc gcgcgatgtg gttaaacgca ttgccgacag cgttgtacga
    24781 ttggcaacat gcagcccgta cagccattgt gaaatcgctg ttaagcaccc acgcgacggc
    24841 ctgtttgatt gttattcgtc tagcgcgaga gacggcgggg tgcgcattaa aaccatgccg
    24901 ctgcctgctg ataaatggga cttaatcccg ctgccgcaat ctgttgccat atcggccagc
    24961 cgcttgttcc accgtacaca cggtgcaggt tacgactggc taggtgcgat tggcgtggta
    25021 ctcaaatcac cacacagcaa aagccgctgg ttttgcagcg aatggtgcgc atatgtaata
    25081 ggctacacta acccgtgccg atacagcccg caaaccctgt atgccgcggt atcaactaaa
    25141 gataggccgt ctgagaaaat ggaggaaaca aagtaattta gaaagtttta aataaagagg
    25201 agcggcgacg tgtctgtgtt gcgagcaccg gcacgccagc caagcagatg taccctgcat
    25261 tgacttcaag gccgctttgc ctagctaggc ggcggtaatt ctaacctaaa cggagttaat
    25321 gcgacatggt ttattatcgt gaattacgtt gtgtctactg caaaaaaact attggccaaa
    25381 ggcagcggta acgtacaaat caagtgtaac cgttgtaaaa cggttaatac tttcagctag
    25441 acaaccatta ctaagaatgc cgtcgagcat cattttaaaa ctgatttcag aacaccagcg
    25501 agagtgtcgg agagtaagta aaaatgatgc aaaaatacca ctcaacggcc ccgctgccat
    25561 tcgtcggaca gaagcgatat ttcattaaac acttcactaa agtattgtcg caaattcccg
    25621 ctgacggcaa acattggaca attgtagacg tattcggcgg cagcggcctg ttggcgcacg
    25681 ttgcaaaacg tatcaaaccg caggcgcggg taatttacaa cgactatgac aactactcag
    25741 accgcctgcg gcacatccca gattacaacc gtttgcgtga acaaatcgcg cagatagttg
    25801 gcggcatccc caaaggctca aggctagacc ctgaacgtac ccgatcagtg caacaaacaa
    25861 tcactaattt ccaaggccac attgatgtgc gcgtactttc atcatggctt ttatttagtg
    25921 ccaaacaggc aaattcgctt gaacaattgc tggggtttga gttctacaat aaggtacgcc
    25981 aatccccata ctccatcgct gccgactatt tagacggcct cgaaatcacc cagcaagact
    26041 ataatctttt gatggctgag catcagcata accccaatac tctgctggta ttagacccgc
    26101 cttatgtgtc cactgctcag ggtgcgtatg ccgctgataa atactttaat atggttagct
    26161 tcctgcgtat gattcagtat atgcgcccac cgtttatcct atttagctcc acccgtagcg
    26221 aggcgctgga ctacttccaa tttttgcaag agtgcgaacc ggacaaatac cggcgcttca
    26281 gcggctacaa tatcgtttca ctagatgcca agatgggcaa aggaatcgag tatcaggaca
    26341 atatgattta taaaatagat taacccgcct aaacggcggg cggtatcaaa gttccacctt
    26401 atagcgcggc ctaactaggt gtcaaagatt ttccgaaaag gtgctaaagt tcgcgccgcc
    26461 ttataaactg cacccgtttt cgggtgcagt tttttattgc ggattgggac gggaatggaa
    26521 aaggatgtgg attttttatt ttaatcggct atacaattgc tgctttcatg ccgtctgaag
    26581 cacgtttcag atggcatcgg tttacatcag ataaggataa aaatgggtat ttacgatttt
    26641 caaatgaaag atgcagaagg caatgcggtt gatttgtcag gctatcgcgg caaggttctg
    26701 ctgattgtca acacggcaac gcgttgcggt ctgaccccgc aatacgaggc tttgcagaag
    26761 ctgtatgcac aatataccgc agaaggcttg gagattttgg attttccgtg caaccagttc
    26821 cgcgaacagg ctcccgaaag cagcggagaa attgcccaag tgtgtatgat gaagtttggc
    26881 acgaagttta agattttcga caaaatcgaa gtcaatggag caaataccgc gcccttgtat
    26941 gcctacctga aatccgtcaa accgcaagat aaaggcaatc atctgtttaa agatttcgtg
    27001 ttgaagctcg cagctttggg agagaagcgc gacgaaggcg acatcaaatg gaactttacc
    27061 aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt ttgcgcctag tgttacgccc
    27121 gaagaaatcg aagccgatat tcgggcattg ctgtaatctg ccgccgtctt gggaaatgcc
    27181 gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt attccgaaat atcggcaaca
    27241 ccgctttcgg ggaagatggg caaatccggc ggtgggagca gggtatccaa agtcagggcg
    27301 gacgcatcga gtgtggggta gccttcaaac gcgaccgaat aaccgctgtt tccgttcata
    27361 cggattcggg cgtgcgcgcc cagagggaaa gtgattttgt cggcaatgtg cccgaaggga
    27421 aagcccgtca gcacggggat tttcgccgtg cgcgaaacat ggttggcaac ggcggaaaaa
    27481 tcataagacg gatcatagac atctcgaatt ttttccatac ggaaattgcc gaacacgatg
    27541 gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat tgagcatacg ttcgatgcgg
    27601 tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc cgtcgatgtc gggcatataa
    27661 ggcgtgccgg cgagcgaggc gaggacgctt aagttgccgc cccacaatat gccttcggtt
    27721 tcgacatcgg cgcgttggat ataaggaaca tcaacggtca ggcggttttg ggttgcaccc
    27781 ttgataaagg catccatcgt aaacgcaccg ggggcgggtt tgccaaaatc gctataagcc
    27841 atcgggccgg caaaactcat catattgcct tttgccaaga gtgccagctg gacggcgcaa
    27901 acatcgctga atccgaaaaa cagcgtacca cgttcgcgca ttcttgcgcc gagtgaggca
    27961 aaatcaatat gcggcagaat gcgtgccgca ccataaccgc cgcgcaaacc catcagaact
    28021 ttcggtatgg caacgcgacc ggaagcgaca tcttggaaat cggcggcacg ttgtgcgtcc
    28081 gtaccggcaa accgttggaa gcggcggctg cccgcctgtt ggttggttac ggtaaaaccg
    28141 gcattgtaaa ggcgggtcaa tgcagtattg acgcggttga tgtcttcggc aaagcctgag
    28201 gaggcgacaa tacgcagaag attgtcggca gaattagaat ggtgtggtgg ttcggggtac
    28261 acagttcggg ctttcgcaac agggtgagag ggcgcggatg gggcattgtt tgaagcaggc
    28321 gcagtaccgc aagcctgaag cagtcccgcg cctgcggcgg cggtacaggt tttcagaaaa
    28381 cggcggcggg aggtcggttc ggtcataatt tccctttcat acggtttcag acggcattgg
    28441 cgggcttaaa gcagcttttt aagctgttcc gtccaatctt gcggcagggt cataccgtgt
    28501 ttccggacgg ggtttgccca aatcggcgcg gggaaggacg catcgttttc aaagcgggcg
    28561 ataatatgcc agtgcaggtg cggcacgaca ttgcccaagc tggcgaggtt gatttttgcc
    28621 ggccggaaca cttggcgcat agcggcttcg actttgtaca ccatttccat caattcgccg
    28681 cgttccgctg ccgaaaggtc ggtcatttcg gcaatatgct tacgccaaat gacgcggcag
    28741 aatgcaggcg aaccgctgtc gttatggacg gcgatgacgc gcagcttggg ggtttgcagc
    28801 aaaacgtctt cattttgtgc cgtgcagatg gggcagggtt gggcggtcat ttttccttat
    28861 tcctgaaagt gtttaaaaaa cggtagggta atttttaata tttcaatctg tctgtccgca
    28921 ttgccgttaa ttgtaccact tcatcgtgtt tccttttcgg ttggaacccc gccctttgtg
    28981 gcggcaggaa cagactttat ttgggagggg tgtaacacct tccaaatcag gacgacacat
    29041 agggcggtgc tttatgtgtc gtcctgtgtg ttgaaacata tcggacgcgt ttgaacagtt
    29101 gtgcggcaaa ctgttttagc ggcaggcaag agatgccgtc tgaagcccat tgtccgtcat
    29161 tcccacacag gcagttgcta cggttgctga agccccttag ccgtcgcgca ggcggttgct
    29221 acggttacta aaatcccgtc attcccgcgc aggcgggaat ccagtccgtt cggtttcagt
    29281 tgtttctgat aaatcttgca gcgttgg
//
LOCUS       DAWKQB010000008        28899 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.008, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000008 DAWKQB010000000
VERSION     DAWKQB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28899
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.008"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            60..740
                     /locus_tag="WJL14_000253"
     CDS             60..740
                     /locus_tag="WJL14_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ7655383.1"
                     /translation="MERYKNAVGKDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDGNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQHNQKWIFRELS
                     NINNFRNRLAHHEPICFKGAIKDTGYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            783..1559
                     /gene="lpxA"
                     /locus_tag="WJL14_000254"
     CDS             783..1559
                     /gene="lpxA"
                     /locus_tag="WJL14_000254"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ7655384.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            1917..3308
                     /locus_tag="WJL14_000255"
     CDS             1917..3308
                     /locus_tag="WJL14_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7655385.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPLMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFMPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMALA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAIVVLGGIKGIAKA
                     ASFIVPAMAVFYVVGGISIIVINSDALMPAVNLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            3387..4643
                     /locus_tag="WJL14_000256"
     CDS             3387..4643
                     /locus_tag="WJL14_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ7655386.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQHCTAARYQ
                     INKERMVRMSEYSREMFRRFEAQTGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQVDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFNNRIRVGGMAELSGYAIKLPEKRRETLAL
                     VVNDLFPEGGDLNQTLFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            4824..5633
                     /gene="zupT"
                     /locus_tag="WJL14_000257"
     CDS             4824..5633
                     /gene="zupT"
                     /locus_tag="WJL14_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ7655387.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            5730..8567
                     /locus_tag="WJL14_000258"
     CDS             5730..8567
                     /locus_tag="WJL14_000258"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ7655388.1"
                     /translation="MLDKYSPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEASRKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADSGEIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVATL
                     PESEDAPVAVCNGARLMLKVEIDKATETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(8938..9327)
                     /locus_tag="WJL14_000259"
     CDS             complement(8938..9327)
                     /locus_tag="WJL14_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7655389.1"
                     /translation="MRYTIKTFQTLADETFPSKNFKILSFNGVARPITIVCPTHGVQT
                     VSAGQAFIRSKHGCPACGLVLSTSVLASRGRSVSILDTATGETLSFPSVQAAAKALNT
                     SYGSIRTKLDGRSSPDNLVCNRYKVMP"
     gene            9713..9904
                     /locus_tag="WJL14_000260"
     CDS             9713..9904
                     /locus_tag="WJL14_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655390.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(9901..10821)
                     /gene="oxyR"
                     /locus_tag="WJL14_000261"
     CDS             complement(9901..10821)
                     /gene="oxyR"
                     /locus_tag="WJL14_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ7655391.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(10825..11088)
                     /gene="minE"
                     /locus_tag="WJL14_000262"
     CDS             complement(10825..11088)
                     /gene="minE"
                     /locus_tag="WJL14_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ7655392.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            complement(11092..11907)
                     /gene="minD"
                     /locus_tag="WJL14_000263"
     CDS             complement(11092..11907)
                     /gene="minD"
                     /locus_tag="WJL14_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ7655393.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            complement(11936..12649)
                     /gene="minC"
                     /locus_tag="WJL14_000264"
     CDS             complement(11936..12649)
                     /gene="minC"
                     /locus_tag="WJL14_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ7655394.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            complement(12796..13164)
                     /gene="rplQ"
                     /locus_tag="WJL14_000265"
     CDS             complement(12796..13164)
                     /gene="rplQ"
                     /locus_tag="WJL14_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ7655395.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            complement(13188..14174)
                     /gene="rpoA"
                     /locus_tag="WJL14_000266"
     CDS             complement(13188..14174)
                     /gene="rpoA"
                     /locus_tag="WJL14_000266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ7655396.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            complement(14200..14820)
                     /gene="rpsD"
                     /locus_tag="WJL14_000267"
     CDS             complement(14200..14820)
                     /gene="rpsD"
                     /locus_tag="WJL14_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ7655397.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            complement(14840..15235)
                     /gene="rpsK"
                     /locus_tag="WJL14_000268"
     CDS             complement(14840..15235)
                     /gene="rpsK"
                     /locus_tag="WJL14_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ7655398.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            complement(15255..15617)
                     /gene="rpsM"
                     /locus_tag="WJL14_000269"
     CDS             complement(15255..15617)
                     /gene="rpsM"
                     /locus_tag="WJL14_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ7655399.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            complement(15683..15796)
                     /gene="rpmJ"
                     /locus_tag="WJL14_000270"
     CDS             complement(15683..15796)
                     /gene="rpmJ"
                     /locus_tag="WJL14_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ7655400.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            complement(15817..16035)
                     /gene="infA"
                     /locus_tag="WJL14_000271"
     CDS             complement(15817..16035)
                     /gene="infA"
                     /locus_tag="WJL14_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ7655401.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            complement(16040..17350)
                     /gene="secY"
                     /locus_tag="WJL14_000272"
     CDS             complement(16040..17350)
                     /gene="secY"
                     /locus_tag="WJL14_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ7655402.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            complement(17362..17796)
                     /gene="rplO"
                     /locus_tag="WJL14_000273"
     CDS             complement(17362..17796)
                     /gene="rplO"
                     /locus_tag="WJL14_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ7655403.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            complement(17798..17983)
                     /gene="rpmD"
                     /locus_tag="WJL14_000274"
     CDS             complement(17798..17983)
                     /gene="rpmD"
                     /locus_tag="WJL14_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ7655404.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            complement(17976..18494)
                     /gene="rpsE"
                     /locus_tag="WJL14_000275"
     CDS             complement(17976..18494)
                     /gene="rpsE"
                     /locus_tag="WJL14_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ7655405.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            complement(18513..18866)
                     /gene="rplR"
                     /locus_tag="WJL14_000276"
     CDS             complement(18513..18866)
                     /gene="rplR"
                     /locus_tag="WJL14_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ7655406.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            complement(18880..19413)
                     /gene="rplF"
                     /locus_tag="WJL14_000277"
     CDS             complement(18880..19413)
                     /gene="rplF"
                     /locus_tag="WJL14_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ7655407.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLTFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            complement(19427..19819)
                     /gene="rpsH"
                     /locus_tag="WJL14_000278"
     CDS             complement(19427..19819)
                     /gene="rpsH"
                     /locus_tag="WJL14_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ7655408.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            complement(19835..20140)
                     /gene="rpsN"
                     /locus_tag="WJL14_000279"
     CDS             complement(19835..20140)
                     /gene="rpsN"
                     /locus_tag="WJL14_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ7655409.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            complement(20143..20682)
                     /gene="rplE"
                     /locus_tag="WJL14_000280"
     CDS             complement(20143..20682)
                     /gene="rplE"
                     /locus_tag="WJL14_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ7655410.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAI
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            complement(20692..21015)
                     /gene="rplX"
                     /locus_tag="WJL14_000281"
     CDS             complement(20692..21015)
                     /gene="rplX"
                     /locus_tag="WJL14_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ7655411.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            complement(21027..21395)
                     /gene="rplN"
                     /locus_tag="WJL14_000282"
     CDS             complement(21027..21395)
                     /gene="rplN"
                     /locus_tag="WJL14_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ7655412.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(21617..21880)
                     /gene="rpsQ"
                     /locus_tag="WJL14_000283"
     CDS             complement(21617..21880)
                     /gene="rpsQ"
                     /locus_tag="WJL14_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ7655413.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            complement(21880..22071)
                     /gene="rpmC"
                     /locus_tag="WJL14_000284"
     CDS             complement(21880..22071)
                     /gene="rpmC"
                     /locus_tag="WJL14_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ7655414.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            complement(22071..22487)
                     /gene="rplP"
                     /locus_tag="WJL14_000285"
     CDS             complement(22071..22487)
                     /gene="rplP"
                     /locus_tag="WJL14_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ7655415.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            complement(22471..23163)
                     /gene="rpsC"
                     /locus_tag="WJL14_000286"
     CDS             complement(22471..23163)
                     /gene="rpsC"
                     /locus_tag="WJL14_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ7655416.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            complement(23173..23502)
                     /gene="rplV"
                     /locus_tag="WJL14_000287"
     CDS             complement(23173..23502)
                     /gene="rplV"
                     /locus_tag="WJL14_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ7655417.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            complement(23511..23789)
                     /gene="rpsS"
                     /locus_tag="WJL14_000288"
     CDS             complement(23511..23789)
                     /gene="rpsS"
                     /locus_tag="WJL14_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ7655418.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            complement(23796..24629)
                     /gene="rplB"
                     /locus_tag="WJL14_000289"
     CDS             complement(23796..24629)
                     /gene="rplB"
                     /locus_tag="WJL14_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ7655419.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            complement(24635..24949)
                     /gene="rplW"
                     /locus_tag="WJL14_000290"
     CDS             complement(24635..24949)
                     /gene="rplW"
                     /locus_tag="WJL14_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ7655420.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            complement(24946..25566)
                     /gene="rplD"
                     /locus_tag="WJL14_000291"
     CDS             complement(24946..25566)
                     /gene="rplD"
                     /locus_tag="WJL14_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ7655421.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            complement(25566..26210)
                     /gene="rplC"
                     /locus_tag="WJL14_000292"
     CDS             complement(25566..26210)
                     /gene="rplC"
                     /locus_tag="WJL14_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ7655422.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            complement(26429..27112)
                     /locus_tag="WJL14_000293"
     CDS             complement(26429..27112)
                     /locus_tag="WJL14_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HEZ7655423.1"
                     /translation="MHRYRISEIGQKVTAAGFRHLDVLLVGATGVGKSSTLNALFGEQ
                     KAKVGTGVDPETQLVESYMLNDVLRFWDSAGLGDGKEADRAHRQKLIDVLSKTYTHSD
                     GQWGWIDLVFVILDGSSRDLGTAYDLLRDVILKMIDPDRVIVAINQADMAMKGRYWDK
                     VLHQPQPNLQQFLDEKAESVQKRILEATGLQISKPVYYSAYENYHLKEIMDAVINHIP
                     VCRRKMHTP"
     gene            complement(27121..28173)
                     /locus_tag="WJL14_000294"
     CDS             complement(27121..28173)
                     /locus_tag="WJL14_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HEZ7655424.1"
                     /translation="MFYSQVNKGKMMSLLNVFKDILNNKELNSEERHDLERAVKILQD
                     THVNILITGATGCGKSSTINALFSIDQATGKSDGLVKEVAKVGVGVDPETMDIAKYEL
                     ENMTIWDSPGLGDGEKADQKHRRNIINKLLEKDSNGNAVIDLVLVLLDGGSRDLGTSY
                     ELINNVIINTLKKSGEGRTDRLLVAINQCDMAMKGRNWDAEAGRPNAALENFLEEKVC
                     STRKRIQEATGVTVNPIYFSAGFKSEDEEQRPYNITRLLRYIITAMPSEKRVIVAEKI
                     NVEEIERDTSRRKQEERQEIDSSIGSALEKILGAAGGVVKDVVSTIASGVGTLIGGAA
                     KAIGGAISTVASFFGW"
     gene            complement(28463..28774)
                     /gene="rpsJ"
                     /locus_tag="WJL14_000295"
     CDS             complement(28463..28774)
                     /gene="rpsJ"
                     /locus_tag="WJL14_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ7655425.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(28792..>28899)
                     /locus_tag="WJL14_000296"
     CDS             complement(28792..>28899)
                     /locus_tag="WJL14_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7655426.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         7928 a         7367 c         6095 g         7509 t
ORIGIN      
        1 aggaatcagg tattagcgtg aattataagg aatgctgtag tattttttct gatttcagaa
       61 tggaacggta taaaaatgcc gttgggaaag acaaagcagc tgaattgtat ttattgaatt
      121 tatctttatc aagagaactt tttcatgttg tttcaatatt tgagattgtt ttaagaaaca
      181 aaattgacat ttgccttcag caggcgttta aagacggaaa ctggttatat aacagcatac
      241 agccacaaac gaatcctgca ttgaaatatc aaggttgctt tttaagaaat ggtacgaaag
      301 aatctgctga attaatcaag gttgcattat ctaaaataca aaataatagc ggaggaaagt
      361 ttgaccacaa ccaattggtc gcaggactag gattcggttt ttggcggtat ctgtttgcgg
      421 gagggaaaga tgcccaattt gatgctacag ggaaagtatt gatgaaagtt tttccaaaaa
      481 agcccaaatc tacaccaagt gtgcaacata accaaaaatg gatttttcga gaactttcca
      541 atattaataa tttccgtaat cgtttggcac atcacgagcc gatttgtttt aaaggtgcaa
      601 taaaagatac cggctacgct agaaatatcc accaatctat ttttgaactg cttaattaca
      661 tggatgtaga tactgcttca gtatttagcc attttagcga tcaagtgatt gcggtctgcg
      721 atgaaatcga caaactgtga ttttctatta tttgtcttaa caagcagcgg agtattaaaa
      781 acatgaccct catccacccg accgccgtca tcgaccccaa agccgaactc gactcaagcg
      841 tcaaagtcgg cgcgtacact gttatcggcc ccaacgtcca aatcggcgcg aacaccgaaa
      901 tcggtccgca cgccgtcatc aacggccaca ccagcatcgg cgaaaacaac cgcattttcc
      961 aatttgccag cctcggcgaa atcccgcagg acaaaaaata ccgcgacgag ccgaccaagc
     1021 tgattatcgg caacggcaac accatccgcg aattcaccac ctttaattta ggcacggtaa
     1081 ccggcatcgg cgaaacccgt atcggcgacg acaactggat tatggcgtac tgccacctcg
     1141 cgcacgactg cgtggtcggc aaccacacca tcttcgccaa caacgcctcg cttgccggac
     1201 acgttaccat cggcgactac gtcgttttgg gcggctacac gctggttttc caattctgcc
     1261 gcatcggcga ctacgccatg accgccttcg ccgcaggcgt acacaaagac gtaccgccct
     1321 actttatggc atcgggctac cgcgccgaac cggcggggct caacagcgaa ggcatgcgcc
     1381 gcaacggttt taccgcagag cagatttccg ccgtcaaaga cgtgtacaaa accctctacc
     1441 atcgcggcat tccgtttgaa gaagccaagg cagacattct ccgccacgcc gaaacccaag
     1501 ccgagcttgc cgtattccga gactttttcg cacaatcggc acgcggcatc atccgctgac
     1561 cgtacccttt tgatgccgtc tgaaaccccg acagcgtttc agacggcatc ggttttttta
     1621 aagcggtata ttgtttgatc gatgcatcat tttgtagatt tcagaagtat ggcagtcggc
     1681 agcattgatg atttctgccg cccgccacat ccccattcgg acatttcatt gtgacctccg
     1741 ccgctgcttt taaagaattg caaacaattt ccgcagcttg aaccggcctg ttttcggcga
     1801 aaaaaaccaa aagtattttt caaaagaaca tattgtctta ttggatattc tgtgctgaaa
     1861 ttactggaca ctacaaaagt ggtgttttaa aaataaaaca gacaaaggag aaagccatgc
     1921 aagtgttttt agacaatccc aaagcctttt ttgaaaccgt cagcggctgg gtttggggac
     1981 ctttgatgct gatgctgctg gtcggcacgg gtattttgct gactgtttta ttaaaaggtt
     2041 tgcagttcac gatgttgggt tatgcgctga aacaggcgtt tatgccgcca aagaagcata
     2101 aaagcggcga aggccacgaa ggcgatattt cccatttcgc ggcgttgatg accgcgctgt
     2161 ccgccaccat cggtacgggt aacatcgccg gcgtggcgac tgcggtggta accggcggcc
     2221 cgggcgcggt attttggatg tggatgaccg ccattttcgg catggccacc aaatacggcg
     2281 aaggcgtgtt ggcggtgaaa taccgcgtca acaattccaa aggcgaaatg tccggcggcc
     2341 cgatgtatta catcgaaaaa ggcttgggca aaaactggaa atggatggcg ttggcgttcg
     2401 ctttattcgg cacattcgct tccttcggta tcggcagctc ggtgcagtcc aactcggttg
     2461 cgcaggcagt gcaaaccagc ttcggtatcg aacctgccta taccggcatt acgttgaccg
     2521 ttctgactgc catcgttgtg ttgggcggta tcaaaggcat cgccaaagcc gcttctttca
     2581 tcgtgcctgc tatggcggtg ttttatgtgg tgggcggcat ctccattatc gtcattaact
     2641 ccgatgcgct gatgcctgcc gtcaacctga ttttctccga tgcgttcagc gcgcaggccg
     2701 tggcaggcgg cgcaatcggt acggtcatcc gctacggcgt ggcgcgcggc gtgttctcca
     2761 atgaggcggg tatgggttct gcgcctattg ccgccgcagc cgcgaaaacc gaccatcctg
     2821 tccgtcaggc cttggtttct atgaccggta cgtttttgga taccatcgtc gtttgctcga
     2881 ttaccggcat cgtgttggta atgggactgc tcggcgcggg cggcgagttt gtcaaacctg
     2941 aagtgagcgg cgcggcactg acaaccgtca cattccaaaa aatgctgccc ggcatcggcg
     3001 gctggattgt taccatcggt ctgattttct ttgcctactc aaccattttg ggctggtgct
     3061 attacggcga aaaatgcgcg gtgtatgttt tcggcgagaa atttgccggt ttgtaccgcg
     3121 tgggttatgt ttcttccgtt atgctgggta ccgtgttgag ccttgatttg gtgtggctgg
     3181 cttcggacac attcaacggc ttgatggcat tgcccaacct gattgcactt ttattgatgg
     3241 cgaaagtcat cgtcaatgaa acgcgcgact tcaaacaaaa aatcacaaac ggcgaattgc
     3301 cgcattaata tccgaggccg tctgaatccg aatattcaga cggcacggca tattaagtct
     3361 gttttgaaaa agtcggaggg agaaacatga aagtgcttgt tttaggtgct ggtgttgccg
     3421 gcgtatcttc cgcgtggtat ctggcagagg caggacatga agtaacggtc atcgaccgcg
     3481 ccgagggcgt ggcgatggaa accagttttg ccaacgcagg ccagctttct tacggctata
     3541 ccacgccttg ggctgcaccc ggtattccga ccaaagcact gaaatggctg tttaaaagcc
     3601 atccgccttt gctgtttcgc cccgacggca gcctgtatca aatcgaatgg ctgtggcaga
     3661 tgctgcaaca ctgcacggca gcgcgctatc aaatcaataa agagcgcatg gtcaggatgt
     3721 ccgaatacag ccgtgaaatg ttccgccgtt ttgaagcgca aaccggcatg aattttgagg
     3781 gacgcaaaaa agggacgttg cagattttcc gccaaaccaa agaagtcgaa gcggcaaaac
     3841 aagacattgc cgttttggaa cgctacggcg tgccgtaccg ccgtctgaag cccgaagaat
     3901 gcgcagaatt cgagcctgcg ctggcacgcg ttaccgccaa aattgccggc ggcctgcacc
     3961 tgcccgcaga cgcgaccggc gactgccgcc tcttcactga aaacctgtac aaattgtgtc
     4021 aggaaaaggg cgtacggttt catttcaacc aaaccatcag ccgcatcgac cacaacgggc
     4081 tgcgcatcaa aaccgttgaa accgaaacag ggcggtttga agcagatgcc gttgtctgcg
     4141 cgctcggctg cttcagcagg acggttttgg cgcaagtgga tctcaatctg ccgatttatc
     4201 ccgtcaaagg ctattccttg actctgccgg tcacaaattc cgacggcgca ccggtgtcca
     4261 ccgttttaga tgagagctac aaagtcgcca tcacgcgctt caacaacaga atccgcgtcg
     4321 gcggcatggc ggaattgtcc ggctacgcaa tcaaactgcc cgaaaaacgc cgcgaaacct
     4381 tggctttggt cgtcaacgac ttgttccccg aaggcggcga tttaaaccaa acattgttct
     4441 ggagcggact cagaccgatg acgcctgaca gcacgccgtt aatcgggcgc acccgctttg
     4501 acaacctgtt tctgaatacc ggccacggta ctttgggctg gaccatgtcg ctgggttcgg
     4561 caaaattaac cgccgatatc gtgagcggca aagacaccga aatccgaagc gacgatttga
     4621 gcctgtcgcg ctatcaggct taagctggaa agcgataagg tttagcgaca tttcaaattt
     4681 aaaggccgtc tgaaaatcat ttttcagacg gccttttgtt tcaaaattcg ggtatccaat
     4741 caataaaata aataataact attatcatac gcattaaaaa gtgttagtct gaacctgtcc
     4801 cagcttttag cgcaggtttt cccatgcccg atttttcgat gtccaatttg gccgttgcct
     4861 tttccattac gttggctgcc ggtttgttta ccgtattagg cagcggcttg gtgatgtttt
     4921 ccaaaacgcc caatccgcgc gtgttgtcgt ttggtttggc atttgccggc ggtgcgatgg
     4981 tgtatgtttc cctgacggag attttcagta agtccagcga ggcgttcgct gaaatttatg
     5041 ataaagacca cgcgtttgcg gcggcgacca tggcattttt ggcagggatg ggcggcattg
     5101 cgctgattga ccgtctggtg ccgaacccgc atgaaacttt agacgcgcaa gacccgtcgt
     5161 ttcaagaaag caaacgccgc catatcgcgc gagtcggcat gatggcggcg tttgcgatta
     5221 ctgcgcacaa tttccccgaa ggcttggcga cgttttttgc cacattggaa aatccagcag
     5281 tcgggatgcc tttggccttg gcgattgcca tccataatat tccggagggc atttccatcg
     5341 ccgcgccggt ttattttgcc acccgcagcc gtaagaaaac ggtgtgggcg tgtctgctat
     5401 ccggcttggc cgagccgttg ggggcggctt tgggctattt ggttttgcag ccgtttttgt
     5461 cgcctgccgt gtttggttcg gtattcggcg tgatagccgg tgtgatggtg tttttggcgt
     5521 tggacgagct gctgccggct gccaaacgct attcagacgg ccatgaaacc gtttacggcc
     5581 tgacaatggg catggcggtg attgccgtca gcctggtatt gttccatttt taaaacctgt
     5641 atgccaaagg ccgtctgaaa tgattcagac ggcctttttg cgttataatc cgcacatcaa
     5701 aaataacctt aatttcacaa agttccatca tgttagacaa atactccccc gccgaaatcg
     5761 aatccaaaca ttatcaaaac tgggaagagc agggctattt ccaacctgat atggatttga
     5821 ccaaaccgtc tttttccatc caactgccgc cgcccaatgt aaccggcacg ctgcacatgg
     5881 gtcatgcctt caaccaaacc attatggacg gcctgacccg ctactaccgt atgaaaggct
     5941 gcaacaccgc ctggattccc ggcaccgacc acgcgggcat cgccacgcaa atcgtggtcg
     6001 agcgtcagct tgccgcgcaa aacgtgtccc gtcatgactt gggtcgtgaa aaattcttgg
     6061 aaaaagtgtg ggaatggaaa gaagtttccg gcggcacgat tacgcagcag atgcgccgtg
     6121 tgggctgttc tgccgactgg acgcgcgagt atttcacaat ggacgatgta cgcgccgaaa
     6181 ccgtaaccga agtgttcgta cgcctgtatg aacagggctt gatttatcgc ggcaaacgct
     6241 tggtgaactg ggatccggta ttgggtaccg cagtgtctga tttggaagtg gaaagcgtgg
     6301 aagaacaagg ctctatgtgg cacatccgct atccgctggc ggacaatcct gccgaagccg
     6361 tcatcgtcgc caccacccgt cccgaaacgc tgttgggcga cgttgccgtt gccgtcaatc
     6421 ccgaagacga acgttatacc cacttaatcg gcaaagaatt aatcctgccg ctgaccggcc
     6481 gcaccattcc cgtgattgcc gacgaatacg ttgaaaaaga cttcggcacc ggctgcgtga
     6541 aaatcacgcc tgcgcacgac ttcaacgact acgaagtcgg caaacgccac gatacgcgct
     6601 tggttaatgt gttcgacttg gaagccaaag tattggcaaa cgccgaagtg ttcaacttca
     6661 aaggcgaagc gcaaccaagc tttgccctgc ctgaaaaata cgcaggcttg gaccgctttg
     6721 ccgcgcgcaa acaaatggtt gccgatttgc aggaacaagg tttcttggtc gaaatcaaag
     6781 cgcacacgct gatgacgccg aaaggcgacc gtaccggttc cgtgattgag ccgatgctga
     6841 ccagccaatg gtttgtcgcc atgtccgcca cgccaaacgg cggcgagcct gacagcgaat
     6901 tcaaaggctt aagccttgct gacaaagcca aaaaagccgt cgacagcggc gcggtgcgct
     6961 ttatccccga aaactgggtc aacacctaca accaatggat gaacaacatc caagactggt
     7021 gcatctcgcg ccaactgtgg tgggggcatc aaatccctgc atggtacgac aacgaaggca
     7081 atgtgtacgt tgcccgcaat caggaagaag ccgaaaaaca agccggcaaa acaggcttga
     7141 cccgagaaga agacgtattg gacacatggt tctcctccgc actcgtgccg ttctccacgc
     7201 tcggctggcc gtctgaaacc gacgaactca aagccttcct gccgtccaac gtcttggtca
     7261 ccggctacga aatcatcttc ttctgggtgg cgcgcatgat tatgatgacc acccacttta
     7321 ccggcaaagt accgtttaaa gacgtgtaca tccacggcat cgtgcgcgac cacgaaggca
     7381 aaaaaatgtc caaatccgaa ggcaacgtca ttgaccctgt ggatttgatc gacggcatcg
     7441 acttggaaaa actgctggtg aaacgtacca ccggtctgcg caaacccgaa accgcgccga
     7501 aagtggaaga agcctcgaga aaactcttcc ccgaaggcat tccgagtatg ggcgcggacg
     7561 ctttgcgctt caccatggcg agctacgcca gcttggggcg cagcgtgaac ttcgacttca
     7621 aacgcgccga aggctaccgc aacttctgca acaaaatctg gaacgccacc aacttcgtct
     7681 tgatgaacac cgaaaaccaa gactgcggct acggcgcaac cgccactgag ccgcgcggct
     7741 attccttccc cgatatgtgg attgtagacc gtctgaatca gaccatcgag caagtaacgc
     7801 aagcctacga aacctaccgc tttgatttgg cagcggaaac cctgtacagc ttcatgtgga
     7861 acgactattg cgactggtat ttagaacttg ccaaagtgca gcttcaaacc ggctgcgcca
     7921 gccgccaacg cgccacacgc cataccttat tgcgcgtact cgaagccgcc ctgcgcctgc
     7981 tgcacccgat tatcccgttc atcaccgaag aactgtggca aaccgtcgct cctatgtgcg
     8041 acgcgaaaac cgccgacagc atcatgctcg cccgcttccc cgaagccgac agcggcgaga
     8101 ttgttcagac ggcattcgag caaatgaccg tgttgcagga tttgattggc gcagtccgca
     8161 acctgcgcgg cgaaatgggc atccagccca acgtaaaagc cccgctgttt gtcgaaagca
     8221 cggacgactt ggcggactac ctcaaatacc tgccgatgat gacccgcctg accgaagcac
     8281 agcaagtcgc caccctgccc gaaagcgaag acgcgcccgt cgccgtctgc aacggcgcgc
     8341 gcctgatgct gaaagtcgaa atcgacaaag ccaccgaaac cgcccgtttg agcaaagaag
     8401 ccgagaagct gcaaaaagcc ttggacaaac tcaacgccaa actctccaaa cccggctaca
     8461 ccgaaaaagc cccggcgcat ttggtggaaa aagacaaagc cgatttggcg gaattggaag
     8521 acaaaatggc aaaagtgcaa actcagttgt cgaagttgaa agactgattt gattgaatca
     8581 acaaggccgt ctgaaaaatg tttttcagac ggcctctttt atagtggatt aacaaaaatc
     8641 aggacaaggc gacgaagccg cagacagtat aatcggtgta gaaatcaaca cctcctgaca
     8701 ggacgttcga cccttgagcc tgtggttaat ttgctgaaaa tgcacaccca aagcagtgcc
     8761 tgcggcagta acgctgggga acgcgagtgt ggagttgcaa cccgtgatga cggtaagcgg
     8821 gcgaatggaa accctagcat gggcaacctt gccaaaattc ttgatttcga catttaaatc
     8881 cataaaaaac aaactcctgc aaaagctagg agtttattat agcacgaagt ttcgagatta
     8941 cggcattact ttgtacctgt tacagacaag gttgtcgggg gacgaacgtc cgtcgagctt
     9001 ggttcgtata gagccgtagg aggtgtttag ggctttggcg gcagcttgta cgctggggaa
     9061 cgagagtgtt tcgcctgtgg cggtgtcgag gatggagact gaccgacccc tagaggccaa
     9121 aacggaggtt gataaaacta aaccacaggc aggacagccg tgtttggaac ggataaatgc
     9181 ttgccccgca gaaacagtct gcaccccgtg tgtagggcaa acaatggtta taggtcgtgc
     9241 caccccattg aacgaaagaa ttttgaagtt cttagagggg aaagtttcat cagcaagggt
     9301 ttgaaatgtt ttaatggtgt accgcataac tcactccgta taagtgcaaa agattttata
     9361 cgaaaacata aaaattttca aattttttga gacctacctg ataccctgcg caagcacaat
     9421 gcaaagaaaa aggctatacg tctttaccaa atctttacat aaattatcag aaaaccactt
     9481 gcacaaaaca acaaggaggc gcataataca ccctgtagtt agatagatag taaggagata
     9541 accaatgact tagcacctgc cccccgcgcc tgcgactagt gggcattact caatcttcac
     9601 acttaaatca cttgcagggc ttaacaaccc atgcagtagt acacacatca acaaatcagg
     9661 ctggtacgcc ttggcaatag tacggcaagg cgaggcaacg ccgtactggt ttttgttaat
     9721 ccactataca tggcattgca tcggcactga tgaaaacatt cacagtgatg caaaagtcga
     9781 atatcttcag gacggcagtt tccgtggcga tgctatactc aaaatcgatg atgacggcaa
     9841 tatcttggca taccgtgttg tcggctcagg gaaatggcgt tttgaaaaca atgcctgcgc
     9901 ctagtcgcgg ataaaactta ccccgtgaag ctgcgactgc ataatcgccg ccttcatcgc
     9961 cgacaacgcc ttcggacgga caaaattgcg gcggtacgcc aatacgaccc gccggcttgg
    10021 cggcgtacct tcaaacggaa taatgctgaa cagcatatga tcgttttcgg tcagcgcggt
    10081 tgccggtaac acgctgattg ccaaaccgct ggcaaccata tggcggattg tattaatcga
    10141 gctgccctgc aatgtattgg tcaacccctg tatacgttgt ttcgccgcca attcggaaca
    10201 gcttgagagt acctgatccc gcatacagtt gccttccgtc agcagcaaaa cctgctcctc
    10261 acccagcatc cgtggcgaaa cggcatccag ttcctcaaat gaatgccctt tcgggacaat
    10321 cacgaaaaac ggttcgtcat acaagggttc ggtaacaatg cccggctctt gaaacggttc
    10381 ggcaacgata atcgcgtcaa cgtccccgcg tttgagcgac tcggtcaaag tatgcgtgta
    10441 attctcttcc aacatcaaag gcattttcgg tgcagtacgg cgcaacgaga caatcagttt
    10501 cggcagcagg tacggcgcaa ccgtaaaaat cagcccgagt ttgaacgcac cctccagctc
    10561 gttttgttct tcatttgcca aatgcctgat aagctctgcc tcttccaaca ccttacgcgc
    10621 ctgtgcaacg atacgttccc ccgcctcggt cgtaataata tcgttgctgc tccggtcaaa
    10681 caaagagacg gcaagctctt cttccaattt cttaatggca atagacaaag tgggctgact
    10741 gacaaaacaa cgccgcgccg ccctgccgaa atgacgttct tgggcgactg cgacgatgta
    10801 ccgcaattcg gttaaggtca tgtcctatac ctttttctgt tccggcaaag taatgttcaa
    10861 ctcaagcaca tccataccat cctgtttttc ctgggaaata cggatattgt ctaatgaaac
    10921 attcacatat ttggacagga cttccatcaa ctctttacgt aaagtcggca ggtaatccgg
    10981 agcctgacct tcttgggcgc gctcttgggc aatgatgatt tgaaggcggt cgcgggcaac
    11041 ggttgccgtt ttctgctttc tgccgaataa aaattcgatt aatgacatac cttatcctcc
    11101 aaacagacgt ttgaagaagc tttttttctc agcttccaag aaacgcattt cacggttctc
    11161 gcccaaaaga cgggcaataa cgtccttata tgcctcggaa gccgccacgc tgtcctgatg
    11221 gatgaccggt tctccggaat tggatgcctg caagacgttt tgggattcag gaatcacacc
    11281 cagcaaagga atatgcagaa tatcgcaaat atcctgtaca gacagcattt cgccttttgc
    11341 cacacgttcg ggagaataac gcgtaatcaa cagatgttct ttaaccgaac cgccttgctc
    11401 tgccttatgg gatttgcttt gcaaaattcc caaaatcctg tcggagtcac gcacactgga
    11461 aacctcagga ttggtcgtta caatggcttc atcagcaaaa tacaacgcca tcaatgcacc
    11521 ctgctcaata ccggcaggag agtcgcaaat aatatactca aagcccattt tcttgccgga
    11581 cagctcctgc atcacttttt ctacgccctc gcgtgtcaaa gcgtctttat cccgagtttg
    11641 agaggcaggc aagataaaca gattctcaca attcttatct ttgattaaag cctggttgag
    11701 cgtcgcctca ccctgaatga cattgatcag gtcataaacg acacgacgct cacaacccat
    11761 aatgaggtcg aggttacgca agcccacatc aaaatcaatt accgcagttt tatatccgcg
    11821 taatgccaaa cctgtcgcaa tactggcact ggtagtcgtt ttaccgacac cgcccttacc
    11881 tgaagttact acaataattt ttgccacaat atttcctttt taaaatatca aacaattact
    11941 ctgagccaat tgcactgata accaatcggt tatcctgcaa caatatctgt accggctgct
    12001 tgtgcagatg gttcggcaaa tcctgttcaa aattacggta aatacccgcc acagaaacca
    12061 gttctgcctg catggagtgg ataaatatgc gggcagaagt atcacccttg gcaccggcca
    12121 aagcacgccc cctcatcggc gcataaatat gtatattgcc atccgcaatc aattccgccc
    12181 cctggctgac cgcccccgta acaatcaaat cgccatcttc ggcataaacc tgctgaccgg
    12241 tacggacagg ggatgtaatc aatactgttt tccttgcttt ctgaccatcc tccgtgttct
    12301 gcacctcaac ctgacccagt tctttaacat tttccgaatg agacagacaa aacagcaaat
    12361 gatacttcat agccgcagca gcccaacgtt cattagaatg cttcagaccc aaaatttgca
    12421 taccatgcct tgaaaacaac gaaaccaatg cagcaagatc caaagactcg ggataatcaa
    12481 actcttgaac atccagcaca aatggaacaa caccagactc ttgaaacttc ttgcccaatt
    12541 tgaccagcac atcttccaaa tcaaacaagt ctgacgtatg caaagatata gacaatacgt
    12601 ccatctttgt cgactttata tcgaaagcat tcattatata aaccatcata gaatttttta
    12661 atgatgtaag tttaccgcgc aaaccttagc tattcaatta atattttaaa catgatagcc
    12721 aataccctat tacctatcag atattgcaaa aataacgcca cctgcttttc ggcagatggc
    12781 gttatatgac taaaattact cagcagccgg tttgtcaacc aattcaacca gtgccagagg
    12841 tgcattatca cctttacgga atccgtattt caacacccga acataaccac cgttacgagc
    12901 agtaaaacga ggacccaaat cgccaaacag ttttactaca acatcacggt cgcgagtacg
    12961 gtcaaatgcc aaacggcggt ttgccaatga cggcttttta cccaatgtaa tcaacggctc
    13021 tactacacgg cgcaattcct tggccttagg cagagttgtt acaatagttt cgtgagtcaa
    13081 taatgaattc gccatattac gcagcattgc agcacgatga ctgctggtac ggtttaattt
    13141 gcgattgcca ttacgatgac gcatatcaat tatcctttaa ttcttcatta aggcttttcc
    13201 aagcctacag gtggccatgc ttccaactta gaacccagtg tcaaaccttt agatgccaat
    13261 acttccttaa tctcattcaa agatttacgt cccaaattcg gcgttttaag aagctcggtt
    13321 tcagtgcgtt gaatcaaatc gccaatataa taaatatcct cagctttcaa acaattagct
    13381 gaacgtactg tcaattccag atcatccacc ggacgcaaaa gaacagggtc gataggaggt
    13441 gctttttctt caacctcctc cacaggcgta ccctgcaaat cagcaaaaat agacatctga
    13501 tcaatcaaaa tacgtgccgc actgcgtaca gcttcctcag gatcaataga accgtcggtt
    13561 tcgatatcca aaaccaactt atcaagatcc gtccgctgct ctacacgtgc aggttcaacc
    13621 tcaaagctaa cacggctgat gggcgaaaag ctcgcatcca actggattgc accaatctga
    13681 cggttctcat cacgaactac ctgacgacct gaaacagatt gataaccacg accttgctct
    13741 actttaattt ccatctcaat ttgaccgtta tcagccaaat gacaaatgac atgaccagga
    13801 ttcagaattt ctacatcatg cggcaactca atatcaccgg cagatacgac acctgaacct
    13861 gatttcttca acacaagttg aacttggcta cgaccatgga gtttaaacac aataccttta
    13921 atattcagca aaatgtcaac aacatcttcc tgaataccat caacagtaga atattcgtgt
    13981 aatacaccgg caatagctac ttcagtagga gcaaaaccat tcatggatga cagtaagata
    14041 cggcgcaaag cattacctaa ggtatgaccg aaaccacgtt caaatggctg catagatact
    14101 tttgcacgag ttgcagaaaa agtatttaca tcaatttgac gaggtttcaa aaattcggtt
    14161 gtgctattct gcatttaact gtccctcact gagctagcat tatttagagt agaactctac
    14221 caccagctgt tcattaatat caccggtcaa ttccgagcga tccggcatgt ttttgaacac
    14281 accctcaagt ttatccgcat ctacagaaac ccaacccggc aagccgattt gagttgccaa
    14341 acccaatgct tcttgaatac gtacctgttt tttggctttt tcacgaactg agacaacatc
    14401 accagctttc acttggaaag aaggaatatt gacaacttgt ccattcacaa ctatcgcctt
    14461 atgagaaaca agctgtcttg cttcagctcg ggtagaaccg aaacccatac gataaacgac
    14521 attatccaaa cgagattcca gcaactgcaa cagcaactcg ccggtagagc ctttacgacg
    14581 atcagcttct gcgaaataac gacggaactg acgttctaat acgccataaa tacggcggat
    14641 tttttgtttt tcacgcaact gcaaaccata gtctgacaaa cgcggttttt ttgcaccatg
    14701 ctgaccggga gcggaatcaa ttttacattt agaatccaaa gagcggcgcg cactcttcaa
    14761 aaacaaatcc gtaccttcgc gacgtgccaa cttacattta gggccaatat aacgtgccat
    14821 gtttcaaatc actccaatat taaatacgac gttttttagg cggacggcaa ccgttatgag
    14881 gcaacggggt aacgtcagta atgctggtaa tcttgaaacc aagagcattc aaagcacgta
    14941 cagaggattc acgacctgga cctggacctt taatacgaac ctctaaattt ttaacgccat
    15001 actcttgggc aactttacca gctgcttctg ctgcaacttg tgctgcaaat ggtgtacttt
    15061 tacgagaacc tttaaaacca gcgccgccag aggtagccca agacaacgca ttgccttgac
    15121 ggtcagtgat tgtaatgatg gtattgttga aagatgcatg aacgtgcaca ataccctcac
    15181 tcacggtttt acgtactttt ttacgtacac gtgaagctgt gtttgcttta gccattaata
    15241 aaattcctta aaatttattt cttaccagca atcgctttac gcggaccttt gcgggtacgc
    15301 gcatttgtac gagtgcgttg accgcggcat ggtaagccgc gacgatgacg gaagccacga
    15361 tagcagccca tgtccatcaa tcgcttgata ctcatagtta cctcacgacg caaatcacct
    15421 tctacttcat acttggcaac ttggtcacgc aaagcatcta attgagtctc gtccaaatct
    15481 tttgctttag tatcaggcgc aatatttgca gcctcacaaa tcaatttagc acgagtagca
    15541 ccaataccgt aaatagcctg aagaccaatt acgatgtgtg cgttattagg gatatttacc
    15601 cctgcaatac gagccatatt ttttcctttt tagggcaaag tgtgtcacta tatcacagaa
    15661 tcacattaaa agaaatattc cattaacctt gacgctgttt gtgacggaga tcagtacaaa
    15721 ttacacgaac tacacgattt cgacgaataa tcttgcaatt tcggcaaatt ttcttaacag
    15781 atggttgtac acgcatttta ttttcctttt attggtttat cttgctctga aaacgattcg
    15841 agccctagtt agatcataag gtgtcagctc tactgtgacc ttatctcccg gagaaatacg
    15901 aatgtaatgc atccgcatct tcccagaaat atgacccaat acaatatggt cattctcaag
    15961 ttttacttta aatgttgcat taggtaaagt ttcaagaatt tcaccttgca tttggatagt
    16021 atcttctttc gccataattc tatttccgag aaaatgattt catttctgaa cgagtcatta
    16081 acttatcata ctgttgagta agcctatacg aatttatttg tgtactaaaa tccatcgtta
    16141 caacaactag aatcaacaaa gacgtgccac ccaaataaaa aggtacattt aaaaccgtag
    16201 ttaagaactc tggaattaaa caaatagttg taatataaag agctccaaac aatgtcaaac
    16261 gtaatacaac tttttctaaa tacctagagg tctgctcacc aggtctaatc ccaggaacaa
    16321 aagcaccact cttttttaaa ttctctgcca tttctttagg gctaaaaacc aaagccgtat
    16381 aaaaataaca aaagaaaata actgtcgctg caaataaagc catatacagc aattgaccgt
    16441 gttgtaacaa tccagctatt ttgtgcaaaa cactatttgt atcagccgaa ccaaaccaac
    16501 ctaaaagagt agatggaaat agaataatac tggaagcaaa aattggggga ataacaccag
    16561 ccatattcaa cttgaaaggc atatgcgtat tttgactacc cgccctacca ttaaactggc
    16621 gttttgcata atgaatagga atcttccgct gtgcactttc aaagtataca accaaataaa
    16681 ttaataataa ggcaccaaat acaataaaca acgccgtaag catgctcata gaaccttggt
    16741 tcgtcagtgt aaccagcttt gcaatacccg aaggaatacc tgaagcaata cctgccgtaa
    16801 tgattaaaga aataccgttc ccgatacccc tttcagtaat ttgctcccca agccacataa
    16861 gaaacatggt tcccgttacc aaagaaacta ccgtggaaac atgaaactca aatgaacttg
    16921 ttacaacaat tccttgctga aatacgaaag atgcaacacc tagactttga agaattgcta
    16981 acaaaacagt accataccta gtatatttcg taattacctt tctaccagcc tccccttctt
    17041 tttttaaagc cttcaatgat ggcaaaattt cagaagcgag ctgtacaata atagaagctg
    17101 aaatatatgg cataattcct attgcaaata tactaaagcg ctctaacgac ccaccggaaa
    17161 acatattcaa tattcccagg atgccgtttc cagcgctttc gtataattta gctaaagcaa
    17221 cagcatcaac tccaggtacg ggtatatggg caccaattcg aaaaacaatc aatgctccaa
    17281 atagaaacaa aagacgtttc ttaagatctc caaatttgga tgaacctgat gacgtttgtt
    17341 gattagccac aatattaaac cttacatttc aatcttacca ccaacagcct cgatagcagc
    17401 tctcgcacct ttggtaactt taataccctt caaagcaact gccttagaaa tttcaccaga
    17461 agcaataact ttaacattag agactgtaga tgcaatcaga cccgcttgct ttaagaccaa
    17521 aatatcaatc tcattaacag caattgattc cagttcactt aaacgaagct gtgcattagc
    17581 tgatgctgtt aaagatttaa aacctctttt agggaggcgt cgttgcaagg gcatttgacc
    17641 accctcgaaa cccaccttat gaaacccacc agaccggctc ttttgacctt tatgaccacg
    17701 accacccgtt ttgccaagac cactaccaat accgcgtcca acacgacgac cagcatgcgt
    17761 agcaccaaca gcaggttgaa ttgtattcaa aaacatatca agactccact ttcaacaagt
    17821 agctgatttt attaatcata ccacggtttt caggggtatc taaaacctct accgtatgct
    17881 cgcgacgacg caaacctaaa ccgcgtgcac atgcacgatg agattcaatt gtaccaatca
    17941 ggcttttaac caatgtaacc ctaatctttt tttgttcagc catggttaac tcccaaaatg
    18001 tcttccactg tcaagccacg tttggctgcg atatcagcag gagtatgcaa cttagacaaa
    18061 ccatctaatg ttgcacgtac gatattatat gggttagtag atccgtgcac tttggcggag
    18121 atattatgaa tgcccatagc atcaaaaacc aaacgcatag gtccaccggc ttttacgcca
    18181 ctaccctctt tagcaggctg cataaatact ttagtagcac catgtcgacc aataacctca
    18241 tgatgaatcg taccattttt taatggtacc ttaatcatag agcgtcgtgc ttgatccatc
    18301 gccttttgga ctgcaacagg cacttctttt gacttacctt tacccatacc aatacgacca
    18361 tctccatcac caacaacagt tagcgcagag aaagccataa tgcgaccacc tttaactact
    18421 ttggttacac ggttaactgc gaccatcttt tcaatcagac cgtcaccgcg ttcttcaatt
    18481 tcatgttttg ccatctgaaa gtctccaaat atttagaagc ttaaaccatt ttcacgagca
    18541 gcttcagcca aagccttcac acgaccgtga tattggaaac ctgaacgatc aaaagcaacc
    18601 ttttctacac ctgctgcttt agccttctca gcgatacgtt taccaactat tgcagctgct
    18661 tcaacattgc ttccagattt cagactaccg cgcacctcag cttccaatgt agaggcttga
    18721 gccaatactt tatcaccttc agcactaatt acttgagcat aaatatgatt attgcttcgg
    18781 aacacacata atcttaccat tttcaagtcc gcaatacgag cacgggtttt gcgtgcacga
    18841 cggagtcggg ttgtatgttt atccattagt gaacctcaat tattttttct tggcttcttt
    18901 cattaccact acttcgccta catagcgaac acctttaccc ttataaggct caggagcacg
    18961 gaacgcacga atctcagcag caacttgacc aacaacttgt ttatccgagc cggttaaaac
    19021 aatctctgtt tggctaggag tttgaacgga gacaccttca ggcatttcat atacgatcgg
    19081 atgagaaaaa cccaaagaca gattcaagat tttaccttgt gcttgagcac gataacccac
    19141 acctatcaat tgcaatcttt tctcaaaacc ttctgaaaca cctttaacca tattgctgac
    19201 taatgcgcga gcagtaccag acattgcatt tgcttgttta ctgctgttat tcgcaacaaa
    19261 agtcaatttg ccatcattaa attcaatggc tacatcagaa tgcaaaggaa aagacaattc
    19321 accgttctta cccttaataa ctaatgcctc tgttccaaat tttacttcta caccagcggg
    19381 aacagtcact gggttttttg cgacacgtga catttctttt cctccactag gctacaatgc
    19441 ataacaactc accaccaaca ccttgagaac gtgctttacg atcagtcatt acacctttag
    19501 aagtactaac aatagcaata cccaagccat tcataacact tggaatctca ctagacgctt
    19561 tataaatacg caaaccaggg cgagatacac gcttgatttg ttcaattaca ggacgacctg
    19621 catagtattt taattgaatt tccaatatag actttacgtc agatgaaact gcgaagtcct
    19681 caatatatcc ttcttctttc aatacctttg caatagcaca ctttaatttt gaagaaggca
    19741 ttgcaaccgc tgctttatta gcacgttgcg cattgcggat acgagtcaac atatcggaaa
    19801 taggatcatg catactcatt cttaattact cctattacca gctggcttta acaacacccg
    19861 gaatttcgcc acgcatggcg atttcacgga ttttaatacg acccaaacca aatttacgga
    19921 aagtaccacg agggcgacct gtcaaagcac aacgacgacg ttgacgcaca ggtgccgcat
    19981 tacgaggaat ggattgaaac ctcaaacgag cctcaaaacg ctcttcctca gttgcattcg
    20041 aatcattgat taccgcttta attgccgcgc gtttagccgc atatttttta gccaaagctt
    20101 gacgtttcag atcacgatta ataagtgctt tcttagccat gattatcctt tgaacggaaa
    20161 tttaaacaat gacaataaag ctttcgcttc ctcatcggtt tttgctgtag tagtaatagt
    20221 aatattcaaa ccacgcaaag catcaatttt atcgtattca atttccggaa aaataatttg
    20281 ctcacgaaca cccatattgt aattgccacg gccatcaaat gatttaccgc tcacaccacg
    20341 gaagtcacgt acgcgaggta atgcaatagt aatcaaacga tccaagaatt caaacatttg
    20401 atcacgacgc aatgttactt tgcaaccaac cggatagtta tcacggattt taaaacctgc
    20461 gatagattta cgggcaacag taacaaccgg tttttgaccg gcaattttct ctaaatcgga
    20521 aacagcatgt tccataactt ttttatcagc aatagcctca cccacaccca tattcaaggt
    20581 gattttttca atacgcggga cttccattac tgatttgtaa ccaaattgtt taaccaattc
    20641 aggaacaact gtctctttat aaaactctct caaccgagcc atgttatctc cttatgcccc
    20701 aatgatagag ccatttgatt tgaagaaacg aacgcgttta actttgcctt cattttcaat
    20761 cagcttaata ccaacacggt ccgctttatt agtttccgga ttcaggattg cgatattaga
    20821 aatatccaaa ggcatttctt tagtaataat accgccctca atgccacgca ttggattagg
    20881 tttttgatgg cgttttacaa cattaacgcc ctcaacaaca actttatcac ccaacactcg
    20941 aactacttga ccctgcttac ctttatcctt accagcaatt actacaaccc tatcgccttt
    21001 aatgatttta ttcatcgtgc cattccttat aatacttcag gtgccaatga aacgattttc
    21061 ataaatcgct cagtacgcaa ttcacgggtt accggaccaa agatacgagt acccaaaggt
    21121 tcaagtttat tattcagtaa cacggcggca ttgttatcga atttaattaa cgcaccatca
    21181 ggacgacgta cacccttagc agtacgaaca actaccgcat tatatacatc gccttttttg
    21241 acacggccac gcggagccgc atctttaact gccactttaa taatatcgcc aacagaagcg
    21301 tagcgacgct tagatccgcc caataccttg atacacatta cgcgacgcgc accagagtta
    21361 tcagccacat ctaagatggt ctgcatttga atcatgttat tacctttaaa ttaaccaact
    21421 taatttacca ctttcagata acttacagca aagccgctgc ttattaaatg aaaccttgcg
    21481 gttcaagtaa accagtcttg gaccccgaaa gggaagaaac ttccagaaga aactgaaaga
    21541 caggaaacga agtttacacg caaattaaat ttgctgcaat acttcgtttc ccattccaag
    21601 cacgttgctt taattcttaa atagaacgtg ctttctcaac cagctcactg acaacccaag
    21661 atttagtttt tgacaatgga cgggattccg atataacaac cacatcacca attccatatt
    21721 gattattttc atcatgggca tggattttag tagataaccg aataatctta ccatacagcg
    21781 gatgttttac tttacgctca accaatactg ttacggtttt atccattttg tcgcttacta
    21841 ctttgccttg caaagtacga acatttttag tttcgctcat tacttagcac ctttttcagt
    21901 taaaacggtt ttaatacgag caatatcgcg acgtacacgt ttcaattcac ttggtttgcc
    21961 taattgaccg gtagcgtttt gcatacgtaa gccaaactga gctttcaaca agtccaacaa
    22021 atctgcattc aactgctcaa cggatttgtc tttcaattca tttgctttca ttattgaccc
    22081 acctgtctta ctacaaaggt tgtaggaata ggcaatttgg cagcagccaa ctcgaatgct
    22141 tcacgagcca gttcctctgg aacgccatcc atttcataca acactttacc tggtttaatt
    22201 tcggcaatgt aatattccac gttaccttta ccgccaccca tacgaacttg aataggcttt
    22261 tcagtaatcg gtttatcagg gaatacacga atccaaatac gaccaccacg tttgatatga
    22321 cgggtcattg cacgacgagc agcttcgatt tgacgggcag tcaaacgacc acgacctacg
    22381 gctttcaagc cgaactcacc gaaacttacc ttattaccgc gagtagcgat gccagtattg
    22441 cgaccctttt gttgcttacg gtatttcagt ctagttggct gcagcattac gtctacctgc
    22501 ctttctttgt ttactctcat gttcaggttt ggaagattta atattgcctt ccgtataaac
    22561 ccaaactttc agacccaata caccatatgt ggtgtgcgct tcgctggttg cataatctac
    22621 atttgcacgt aaagtatgca gtggcacgcg accttcacga taccattcgc tacgggcaat
    22681 atccgcacca ttcagacggc ctgaagtcat aatcttaatg cctttagcac cagaacgcat
    22741 tgcattttgc attgctcgtt tcatagcacg acggaattga acgcgctttt ccaactgctg
    22801 ggcaataccg tcagcaataa tttgagcatc caactcagga cggcgaatct cttcaatatt
    22861 tacatgaaca ggtacaccca tcaagacttg caagtcacgt ttcaaaacct cgatatcctc
    22921 acctttttta cctataacca cacccggacg agcggagtga atggtaatgc gtgcagattt
    22981 tgcagggcgt tcaataacca ctcgaccaac cgaagcattg gccaattttt gacgcaaata
    23041 attgcgaaca tcgatatcct gcttcaaaac agtagaaaag tcggtgcttt tagcaaacca
    23101 ttttgaagcc cagtctttag ttaccgccag gcgaaagcct gtagggttaa tcttttgtcc
    23161 catagctttt ccttagttac ccactgtcac attgatatga caagtttgtt tttcgatgcg
    23221 gttaccgcga cctttggcgc gagcttgaaa acgtttcaag cttgggcctt tgtcaacaaa
    23281 gatagttacc actttcagtt catcaatgtc cgcaccgtta ttgtgctcgg cattagcaat
    23341 agctgactcc aatacttttt taatcagctc ggcacctttt ttaggactga aagccaaaat
    23401 attcaaagct tgggcaacgt ctttaccacg aatcaaatca gctaccaaac gagccttttg
    23461 agcagagata cgggcatttt tatgttgtgc atttactctc atgattcacc ttatttcttt
    23521 ttagcctttt tatcggccaa gtggccttta aaggtacggg tcaatgagaa ttcgcctaat
    23581 ttatgaccaa ccatattgtc gctgataaac acaggcacat gggtgcggcc gttgtgcaca
    23641 gcaatggtca gaccgataaa atcaggcaga atggtagaac gacgagacca ggttttaatc
    23701 gggcgtttgt cgttgcttgc gcgagcagca tctacttttt tcagcaaatg caggtctaca
    23761 tatgggcctt ttttcaatga acgagccata ctaaattaac ctttatttga gtaacggcga
    23821 cgaacaatca tgttatccgt gcgtttgtta ttacgagtgc ggtagccttt agcaggagta
    23881 ccccatgggc tgaccggttc gcgggcctcg cccgtacggc cttcaccacc accatgcggg
    23941 tgatcgacag gattcatgac aacaccgcgt acggtcggac gaataccgcg ccaacggttg
    24001 gcaccggctt taccgatttt tttcaggctt tgctcttcgt taccaacttc accaatggtc
    24061 gcacggcaat ttacgttgat tttacggact tcgccagagc gcagacggac ttgagcgtac
    24121 gcgccttctt tagccagcaa taccgcagaa gcaccggcag aacgtgcgat ttgagcacct
    24181 ttaccgggtt tcatttcgat acagtggata gtcgtaccaa cgggaatgtt gcgaatcggc
    24241 agggtgttgc ctactttgat ggcagcttca gcaccggaaa ccaatacagc accggcttga
    24301 ataccgcgag gagcaatgat gtaacgacgc tcgccgtctg catagcacaa cagtgcgatg
    24361 aaggcagtac ggttaggatc gtattcgata cgctctactt tggcaggaat gccgtctttg
    24421 ttacgtttaa agtctacaac acggtaatgg tgtttatgac cgccgccttt gtgacgggtg
    24481 gtgatatgac cattattgtt acggccggca gtagaatttt tcttttcaag caagggcgca
    24541 taaggtgcac ctttgtgcaa accttctgtt accacgcgaa ccatgccgcg acggcctgca
    24601 gaggtcggct tcattttaac gattgccatt ttgtttattc cttatctgca gctgcagcag
    24661 cggcttccaa atccaactct tgaccggcag ccaagcttac ataagctttt ttaacatcgc
    24721 tgcggcggcc caaagtacga ccaaaacgct tagttttgcc tttagttgta acggtagtta
    24781 cagaagcaac ttgaacacca aacagcagct caacagcagc tttgatttca ggtttggttg
    24841 catttgccaa gactttaaac gtcatttggt tgcgtttttc agccaatacg ttgctttttt
    24901 cagaaacgat aggtgccaaa atcacttgag tcaaacgttg ttgattcata cccattgctc
    24961 ctctaattgt gcaactgcat ctttagtgat gattactttt ttgtaacgca gcaagctgta
    25021 aggatcaact tgttgagctt ccaaaaccaa cacgtttggc aagttgcgtg aagccaagta
    25081 aacattctcg tcgagctgtt tggttacaaa caacacttgc tccagaccca gatttttcac
    25141 ttgttcagca aaaactttgg ttttaggagt ttcagcagtc aacgcctcaa tcgcaaacaa
    25201 acgctcgtca cgagtcaatt gggacagaat agtcgccata ccggcacggt acattttgcg
    25261 gtttactttt tgagtgaagt tttcgtcggg tttgttcggg aacgcgcgac cacctttacg
    25321 ccacagcgga gatgaagtca taccggaacg ggcgcggccg gtaccttttt gacgccatgg
    25381 ttttttggtt gagtgtttta cttcggcacg ggttttttga gcacggttac cagagcgggc
    25441 gtttgccaag taggcattta ccagctgatg aaccaacgct tcattgtatt cgcgggcgaa
    25501 caaagcatca gaaacagaca ggctgcctga aacttgtcct ttagcgtcaa ttactttcaa
    25561 ttccattacg cacctacttt cacgctggga cgaactacaa catcgctgtt gaccgcaccc
    25621 ggaacagcac ccttaaccaa cagcagttgg cgttctgcat caacacggac aacttccaat
    25681 ttttgaacag ttgctttggt gttgccgtat tggccggcca tgcgtttacc ggggaacacg
    25741 cgacccgggt cttgcgccat accgatagag cctggaacac ggtgagaacg ggagttaccg
    25801 tgggaagtac gttgggcacc gaagttatga cgtttgatcg tgccggagaa acctttacct
    25861 ttagaggtac cggttacatc gaccagttga ccgacttcaa acatagaaac ggtgatttcg
    25921 tcaccagctt tcaattcagc cagtttttct tcagtcaaag caaactcaat caaaccgcga
    25981 ccggcttcaa cacctgcttt tgcaaagtgc ccggcttcgg ctttgttgac acgattggct
    26041 tttttctgac caaaggtaac ttgaacggca gtatagccgt cagtatcttt ggattttact
    26101 tgtgtaacgc ggttggcaga catatccaaa acggttaccg gaacagaaac accctgttcg
    26161 tcgaacacgc gggtcatacc aactttgcgt ccaaccagac ctaaagtcat gattattttc
    26221 cttttaaagt aaagggacag gctacgattg gcctgtcttt cagacaaagt taaacttggt
    26281 acgaatgtac caagtcagac attatatctg acaagtcaaa gaaatatcaa caaaaatatc
    26341 aaaaatatct tcaatatgcc gtctgaagcg ttttgaatct tcagacggca tagggtcgga
    26401 tgcttttaaa ggagtcggta tccgaccatc acggcgtatg catctttctc cgacaaaccg
    26461 ggatatggtt gatgactgca tccattattt ctttgagatg gtagttttcg tatgccgaat
    26521 agtaaacggg tttggaaatc tgcaaaccgg ttgcttccag aatccttttt tgtaccgatt
    26581 cggccttctc gtctagaaac tgttgcaaat tcggctgcgg ctgatgcagc accttatccc
    26641 aataacgtcc tttcatcgcc atatccgcct gattgattgc aacaataacc ctatccggat
    26701 caatcatttt aaggataaca tccctcaaca aatcataggc cgtacccaaa tcgcgggagc
    26761 tgccgtcaag gataacgaac accaaatcta tccagcccca ctgcccatcc gaatgagtat
    26821 aggttttaga caggacatca atcagttttt gccgatgggc gcggtcggct tctttcccat
    26881 cgcccaaacc tgcgctgtcc caaaaccgca acacatcgtt cagcatatag ctttcgacca
    26941 attgcgtttc gggatccacg cctgtgccta ccttggcttt ttgttcgcca aacaaggcat
    27001 ttaaggttga ggacttgccg acacctgtcg caccgacaag caagacatcc aaatgacgga
    27061 aaccggctgc tgtgactttt tgcccgattt cagaaatacg gtaacgatgc atatgcgccc
    27121 ctaccagcca aaaaaagaag caaccgtgct aatcgcccct ccaatcgctt ttgcagcacc
    27181 gccgatcagc gtcccgacac cgcttgctat cgttgaaaca acatctttaa ccacgcctcc
    27241 cgcagccccc aatattttct ccagcgcact gccgatacta ctgtcgattt cttgccgctc
    27301 ctcctgtttg cggcggctgg tatctcgttc gatttcttcc acattaatct tctctgccac
    27361 aatcacccgt ttttcagacg gcatagccgt aatgatgtaa cgcaacaggc gggttatgtt
    27421 atagggacgc tgctcctcat cctcactctt aaagcctgct gaaaaataaa tcggattgac
    27481 cgtaacgccg gtagcttcct gaatgcgctt gcgtgtcgaa cacactttct cctccaaaaa
    27541 gttttccaag gcggcgttcg gacggccggc ttccgcatcc caattccgcc ccttcatcgc
    27601 catatcacac tgattaatcg caaccagcaa ccggtctgta cggccttccc cactcttctt
    27661 caatgtattg atgattacat tgttaatcag ttcgtatgat gtccccaagt ctctggagcc
    27721 gccgtcaagc agcaccaaaa ccaagtcgat gacggcattg ccgttactgt ctttctccag
    27781 cagcttattg atgatattgc gccggtgctt ttggtctgcc ttctctccgt cgcccaagcc
    27841 cggactatcc caaatcgtca tattctccaa ttcatatttg gcaatatcca tagtttcagg
    27901 atctacaccc acgcctactt ttgcgacttc ctttactagt ccatcactct ttcccgttgc
    27961 ctgatctatg ctgaacaagg cattgatggt ggatgatttt ccgcaaccgg tggctccggt
    28021 aatcaaaata ttgacatgcg tatcctgcaa gatcttaacc gcccgctcca aatcatgccg
    28081 ttcttccgaa ttcaattctt tgttgtttaa aatatcttta aagacattaa gtaagctcat
    28141 catctttcct ttatttactt gagaataaaa catcatccat ccttagaatc agatggatgc
    28201 ttctgcccaa accaagcccg attctacctt tcttcggggg gggcaagcct tcggaaggct
    28261 atggataaaa aatatcctgt tttgttcact aaaaaatgcc gtctgaaaat tcttgaggca
    28321 caaaataagc cgatttcgcc gaccgcactg ttttttgatt ttgttgtttt gccttaaatc
    28381 tgtttgccgc cgcatatttt cagacggcat aacataaaaa atccccaagc attaattgct
    28441 tggggatttt caacagccct aattattgga ctttgatttc tacatcaaca ccggccggca
    28501 aatccagctt catcagcgca tcggtagttt tgtcggtcca atccacgatg tccatcaggc
    28561 gcaagtgggt gcggatttcc aattgttcac gggaagtttt gttcacgtgc ggagaacgca
    28621 gaatgttgaa acgttcgatt ttggtcggca aaggaatcgg gccttttaca actgcaccgg
    28681 tacgttttgc agtttcaacg atttcttgtg cagaacggtc aatcagggcg taatcataag
    28741 ctttcaggcg gatacggatt ttttggtttg ccatttatca atatccttca attaagcgat
    28801 aacagaagaa accacgccgg cacccacggt acggccgcct tcgcgaatcg caaagcgcaa
    28861 accttcttcc atagcgatag gcgcaatcag ttctacggt
//
LOCUS       DAWKQB010000009        28762 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.009, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000009 DAWKQB010000000
VERSION     DAWKQB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28762
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.009"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(38..598)
                     /locus_tag="WJL14_000297"
     CDS             complement(38..598)
                     /locus_tag="WJL14_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655427.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(804..1196)
                     /locus_tag="WJL14_000298"
     CDS             complement(804..1196)
                     /locus_tag="WJL14_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7655428.1"
                     /translation="MAKSFNQAASELTDIFPNISLTDFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(1313..1723)
                     /locus_tag="WJL14_000299"
     CDS             complement(1313..1723)
                     /locus_tag="WJL14_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7655429.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(1737..2174)
                     /locus_tag="WJL14_000300"
     CDS             complement(1737..2174)
                     /locus_tag="WJL14_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7655430.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(2586..3884)
                     /locus_tag="WJL14_000301"
     CDS             complement(2586..3884)
                     /locus_tag="WJL14_000301"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ7655431.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVFAYYNVQLQHLHNAESVKAEDVMAVIEKVAPRITPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWSESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(3987..5138)
                     /locus_tag="WJL14_000302"
     CDS             complement(3987..5138)
                     /locus_tag="WJL14_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7655432.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAHEAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            5473..6852
                     /gene="norM"
                     /locus_tag="WJL14_000303"
     CDS             5473..6852
                     /gene="norM"
                     /locus_tag="WJL14_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ7655433.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVSGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            7030..8070
                     /gene="murB"
                     /locus_tag="WJL14_000304"
     CDS             7030..8070
                     /gene="murB"
                     /locus_tag="WJL14_000304"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ7655434.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSADRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            8082..8732
                     /locus_tag="WJL14_000305"
     CDS             8082..8732
                     /locus_tag="WJL14_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ7655435.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            8787..9563
                     /locus_tag="WJL14_000306"
     CDS             8787..9563
                     /locus_tag="WJL14_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7655436.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            9614..10180
                     /locus_tag="WJL14_000307"
     CDS             9614..10180
                     /locus_tag="WJL14_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ7655437.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            10200..11090
                     /locus_tag="WJL14_000308"
     CDS             10200..11090
                     /locus_tag="WJL14_000308"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ7655438.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            11115..11873
                     /locus_tag="WJL14_000309"
     CDS             11115..11873
                     /locus_tag="WJL14_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7655439.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(11928..12593)
                     /locus_tag="WJL14_000310"
     CDS             complement(11928..12593)
                     /locus_tag="WJL14_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7655440.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(12793..13689)
                     /locus_tag="WJL14_000311"
     CDS             complement(12793..13689)
                     /locus_tag="WJL14_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ7655441.1"
                     /translation="MQLLKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTIARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRKLTTEMVALLDSRAGKISVSFPFFRKKTAPVSGIRSLLD
                     YDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            13758..14915
                     /locus_tag="WJL14_000312"
     CDS             13758..14915
                     /locus_tag="WJL14_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ7655442.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKVLAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAKTQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHDVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(14978..15979)
                     /gene="hemB"
                     /locus_tag="WJL14_000313"
     CDS             complement(14978..15979)
                     /gene="hemB"
                     /locus_tag="WJL14_000313"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ7655443.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(16162..16446)
                     /gene="yhbY"
                     /locus_tag="WJL14_000314"
     CDS             complement(16162..16446)
                     /gene="yhbY"
                     /locus_tag="WJL14_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ7655444.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            16556..17176
                     /locus_tag="WJL14_000315"
     CDS             16556..17176
                     /locus_tag="WJL14_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ7655445.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            17240..19207
                     /gene="ftsH"
                     /locus_tag="WJL14_000316"
     CDS             17240..19207
                     /gene="ftsH"
                     /locus_tag="WJL14_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ7655446.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASSESEQRPENKA"
     gene            19429..19860
                     /locus_tag="WJL14_000317"
     CDS             19429..19860
                     /locus_tag="WJL14_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ7655447.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFSHLSATLVEAFVKEADRRYA"
     gene            19853..20131
                     /locus_tag="WJL14_000318"
     CDS             19853..20131
                     /locus_tag="WJL14_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ7655448.1"
                     /translation="MLEIEIVYGLPDRQVLKTMRLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            20184..20762
                     /gene="pth"
                     /locus_tag="WJL14_000319"
     CDS             20184..20762
                     /gene="pth"
                     /locus_tag="WJL14_000319"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ7655449.1"
                     /translation="MSNKIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            20823..21029
                     /locus_tag="WJL14_000320"
     CDS             20823..21029
                     /locus_tag="WJL14_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ7655450.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            21026..21388
                     /locus_tag="WJL14_000321"
     CDS             21026..21388
                     /locus_tag="WJL14_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ7655451.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGAALALFTARAACRPD"
     gene            21618..23033
                     /locus_tag="WJL14_000322"
     CDS             21618..23033
                     /locus_tag="WJL14_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEZ7655452.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            23152..23661
                     /locus_tag="WJL14_000323"
     CDS             23152..23661
                     /locus_tag="WJL14_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7655453.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSAGAQFFINT
                     ADNAFLNHRSKEMYGRTVVQDWGYAVFGKVVGGFDVVDAIEGVSTKRHGYHDDVPTEP
                     VIITKAEAV"
     gene            23824..25206
                     /locus_tag="WJL14_000324"
     CDS             23824..25206
                     /locus_tag="WJL14_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ7655454.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGLTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNSDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLNRNPKAK
                     VIFDVKSTRLLTPWIKEHGGEPVMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGMYAGARLLEILSAFDNPSEVLNKLPQNISTPELNIDLPEGSNGHKVIEELTAN
                     AKFEGATEVITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(25226..25981)
                     /locus_tag="WJL14_000325"
     CDS             complement(25226..25981)
                     /locus_tag="WJL14_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7655455.1"
                     /translation="MIKIRNIHKTFGKNTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(25991..26707)
                     /locus_tag="WJL14_000326"
     CDS             complement(25991..26707)
                     /locus_tag="WJL14_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7655456.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(26697..27524)
                     /locus_tag="WJL14_000327"
     CDS             complement(26697..27524)
                     /locus_tag="WJL14_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7655457.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            27809..28717
                     /locus_tag="WJL14_000328"
     CDS             27809..28717
                     /locus_tag="WJL14_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ7655458.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGHDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLLHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         6926 a         8292 c         7145 g         6399 t
ORIGIN      
        1 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atcccctata tcagataaaa
       61 cagcggtaaa attgccgtct gaaaccaact ctgcgccata cgggacacgc tctgcacgtc
      121 tatttgcctc cgatatatcc tccctactgt aggaacgtct gccgatttgc acgaaatcgg
      181 cacctctagc acccttcagg atggcggacg ttatccgttc ttgggattct gcaagacggt
      241 cgacggcatt atacagacgc tcggtctgat acccttccga agcaacctgc ttcaggtatt
      301 ccaaataacc gtaacccaac gcccctccga caagcagggt cgtcaacgct accagcgttg
      361 ccgaacggat tttagggggc atatcttgaa gcatatctct accgaaccga accaaagtct
      421 ctttaaagtc ggactctaca cgcgtagaac cctcggaaat cttgaagttc aactcatact
      481 gcttccgttt gtccgctgtc aaccgcttga ggttgtcatc cccgtattgg acggttgaca
      541 caaccctgta cagactgtcc tgtatttcga ccagcgtctg tgcaaagttg gcggtcataa
      601 ctggattccc aacccttgcc gccgattctg atactcaaaa cggtatccag gcgttccagc
      661 agggcttcgg gagacagcag tgcgtattct tcaatatttt ccataggttg attatagcag
      721 aaccttatac ctgttacaga caaggttgtc gggatttgaa cgtccgtcga gcttggttcg
      781 taggatttag ggtgttgtga accttaccta tcgtacccaa gcactttgta cctgtttgaa
      841 ataaggttgt cgggcgacgt tcgacccttg agcctgtgat tgatttgttg gaaatgtaca
      901 cctaaagcag cacctgcggc agtaacgctt gggaacgtga gtgtttcgtt ggtcgttgtg
      961 tcgaggatga ggaggggctt ccctacattt ggaggtgttt gggtttttga catcttagca
     1021 cactcaggac acccgtactt tgactttata agggcattaa atgtcgaata acgaacgttc
     1081 ccgtgcatag gacaattaac ggttactggg taattcacac cgtcaaagtc ggttagagag
     1141 atattaggga atatatcagt aagttcagaa gctgcttggt tgaaagattt ggccatttga
     1201 gactccaaaa ttgattgtca aatggattta atagcaaatt tataaaaatg tcaaattttt
     1261 cgagaagcac ctgatagtgg cgaggggtat acactgttaa aaagtgtata cgtcatttag
     1321 tgcagatgtg aaccttatac ctccctgcaa gtaaagtctc tgtgtgaacc cgacctttta
     1381 tacggtcagt gatagctgct gggtttatac ccaatgacct tgcagcagat gctcttgagg
     1441 ggaatgtaag tgtttcgcca gttacagtat cctccaatac tgtattttta gataatttat
     1501 ctattgtatc ttctggaatt ttgtgggaat tgacaccata agatgcacat tttggacacc
     1561 cacttttaga gtccaacata cttttgaatg tagagcaagt aaccctcccg tgtataggac
     1621 agacgattac cgtcgggtaa cgaacgccat tgaactccac aagattcaaa tgtggaaatt
     1681 tagaggttag cttagaaact gcttgagtaa atgtaagtgc catagtggtt gtgaacttac
     1741 tttaccaacc tataacgata cctaatcaat cggtcaaacg gagtgcgtcc gcttaaacgt
     1801 acagctaaag tgctgttgga gcaaccgata aatttactgg cagccagtgt acttgcaaac
     1861 tcgtagattt cccctgtctc tgtgtcctcc aacctaatgg caacggcgtt tggagagggc
     1921 ttttcgggat ttcgagtaca ttcaggacat cctgttgagg atttcatggc agcttgtaga
     1981 ttcccgtaaa caaccgaccc atgcaaaggg cattggattg ttaccggttc gtagttttta
     2041 gtgaacttca aaaccgtctt atcaggaaat ttactggaaa accttaactg aacatcttgt
     2101 agcgagagtg attttggcat tatagacctt tctaaaatga aagcattata caaccaaaat
     2161 atagaaattt tcaaattttc tctgaacgca ctggtatccg atggagctaa atgtaaagaa
     2221 aaatggtata cgtctttact aaatctttac acattttaac tgtatagtgt ttgacaaaca
     2281 acaggcatag gcgcataatg cacccatagt tagatagata gtaaggagat aaccaatgac
     2341 ttagcacctg cccccctctc tctcgcgcac cccgcccctc gcttgcggct aatgggcttt
     2401 gcgcgacata tacactttca tctcctgcat ggcttaactg cctatgcaat agtacacaca
     2461 tcaacaaatc aggctggcac gtcttggcat tggctgacgt accattgtag cccgaaatgt
     2521 tgatacccta ctatttatga ccaacggcgg attagggctt cagacggcaa ttttactgca
     2581 aaaccttatg cgaacggatg atgcagcaca atcgtttctt cgcggtcggg gccggtggag
     2641 acgatggcga ccggcgcacc gcagacttct tcaatccgtt tcaaatatgc tttggcattg
     2701 gcaggtaatg cgtcgtagct tttcacgccg aaagtggatt cgctccaacc gggcatggtt
     2761 tcgtaaatcg gcttgcaggc ttctaccgca tcagagccgc aaggcaggat gtcggttttg
     2821 ccgccgtcgg gcaattcgta gccgacgcag atattgatgg tttcaacgcc gtccattaca
     2881 tcgagtttgg taatacacat accggaaatg ccgttgattt ggatggagcg tttcagggcg
     2941 gcggcatcaa accagccgca gcggcgtgcg cgtccggtta ccgaaccgaa ttcgtgtccg
     3001 cgttctgcca aacctgcgcc gacttcgtcg aacaattcgg tcgggaacgg gcccgaaccg
     3061 acgcgcgtgg tataggcttt gacgatgccc aaaacataat ccagcatttg agggcctacg
     3121 cccgcgcctg ccgaagccgc gccggcgagg cagttggacg aagtaacgaa ggggtaagtg
     3181 ccgtagtcga tgtccaacaa cgcaccttgc gcgccttcaa acagcagttt ttcgccgttt
     3241 ttgttttttt cgttcaacac gcgggacacg tcggtaatca tcggcgtaat gcgcggcgcg
     3301 actttttcga taaccgccat cacgtcttcc gctttcaccg actcggcatt gtgcagatgt
     3361 tgcagttgga cgttgtaata agcaaagacg gcatccagtt tttcacgcag tttttcagga
     3421 tgcagcaaat cggcggcgcg aatggcgcgg cgtgccactt tgtcttcgta ggcagggccg
     3481 atgccgcggc cggtcgtgcc gattttgcct ttgccgcgcg atgcttctcg ggcttggtcg
     3541 agcgcgatgt gataaggcag gatcagcggg caggtcggcg cgattttcag acggccttcg
     3601 acgtttttca cgcctgccgc gttcaactcg tcgatttcgc ccaacagggc ttcgggggag
     3661 acgacaacgc ccgaaccgat gaagcagtcc agactttcat gcaggatgcc gctcggaatc
     3721 aggcgcaaaa tggttttttt gccgccgaca accaaggtat gacccgcatt gtggccgcct
     3781 tggaagcgca ccacgcctcc ggcttcttcc gccagccagt caacgatttt acctttaccc
     3841 tcgtcgcccc actgtgcgcc gattactaca acatttttag ccatagccat ataaccttcg
     3901 atattaaaaa ttattgcgga aatccccaaa ccggtgcggc tttgcgcccc gccacgattt
     3961 gcctttcatc cgccatgaac gggtatttag cgtttcacga cctgccaaac gccgtccgtc
     4021 tttttcagac ggcctgcaag ctcttccgaa acattgtgtc cgataccgta atcgattacg
     4081 acacactgcc cttgttcacg caaggcttcg accgcttcgt gcgccgcttc ggcatcttcc
     4141 gcatcgacca acacggcggg ctgccgttcg atggcgggca aacgcccgat aaagctgcgc
     4201 aagtcgaaac tgaatcccgt tgccgggcgc gccctgccga aatatccgcc caatccgtca
     4261 taacgcccgc cgcgcgcgac cgcgtcgtgg aaatcggcgg cataggcggc atacagcaag
     4321 cccgtgtggt aattgtcgac gcgcagctcg gacaagtcga tatggatttc acaatcgggg
     4381 aatgcgtcgc acaccgcctg caattcgccc aacgcgccgc cgaccgccga caaatccggc
     4441 aaccgtccgc gcgcgtcgga caacacttca cgcccgccgt acaggcgcgg cagcagcgag
     4501 aatgcttttg cccacatacc gtccagcttc caagccttga cctgcgcttc gaccgtctcg
     4561 gtatctttat cctgcatcaa ggcaagcagc gttgcagact gccccgcatc caaatgcgcc
     4621 gcatcggaca aggcgcgaaa tatgccgata tgccccagcg aaagcagcac tttgcccata
     4681 tcggcaattt tcatgctttt cagcatcagg tctatcagct cgatgtcgcc acggatgtcg
     4741 gcaaaaccgt acatttccgc ccctgcctgc aagggttcgc gcatattcag cagaccgtcg
     4801 gactgcgcgt gcaacaccgg accggcataa cacaaccggt taatcccttg gttggcggat
     4861 aaaagatggg catcgatacg cgccacctgc ggcgtgatgt cggcgcgtat gcccaactgc
     4921 ctgccgctga gcctgtccgt taccaaaatg gttttcaggg aaagccccgc atcgatatgc
     4981 gtcagcaggg aatgtgcgta ctccatcagc ggaggctgta ccagttcata accgtgtacg
     5041 cggaacagtg ccaacaactg ctccctcgcg ctttcaagct gccgcgcgtt cgtgggcagt
     5101 acgtcggcga tatgttccgg aagctgccat gtctgcatga gaatctgcct tctattttat
     5161 catgtcaata caaaaggctg cccgatccgc aatcagacgg catctttgcc gcaacgcatc
     5221 acgccgctgt caaaacggcg atgcttcctt ccgattcaga atccgaacag cccctgtccg
     5281 acggctcaat gccgtcaagt cgttaaaacc aaactttacc ataaaataca cacaatctga
     5341 atatactttt ataccctgta tccgccgtct gacggcacgg cattttttta ctatataatg
     5401 tgcgccatta tttctcatca cttcctgctg ccgttttctg cttcagacgg catttttgtt
     5461 gattgaacac ttatgctgct cgacctcaac cgcttttcct tttccgtctt cctgaaagaa
     5521 gtccgcctgc tgaccgctct tgccctgccc atgctgttgg cgcaggtcgc gcaggtgggc
     5581 atcggttttg tcgataccgt gatggcgggc ggtgcgggca aggaagactt ggcggcggtg
     5641 gctttgggca gcagcgcgtt tgccacggtt tatattacct ttatgggcat tatggcggcg
     5701 ctgaacccga tgattgccca gctttacggc gcgggtaaaa ccgacgaagt gggcgaaacg
     5761 ggacggcagg ggatttggtt cgggctgttt ttgggcgtgt tcggcatggt cttgatgtgg
     5821 gcggcgatta cgccgttccg caactggctg accttgagcg attatgtgga aggcacaatg
     5881 gcgcagtata tgctgttcac cagcttggcg atgccggcgg caatggtaca ccgcgcactg
     5941 cacgcctacg cctccagcct gaaccgcccg cgcctgatta tgttggtcag ctttgcggcg
     6001 tttgtgttga acgtgccgct gaactatatt ttcgtttacg gcaaattcgg tatgcccgct
     6061 ttgggcggcg caggctgcgg actggcgacg atggcggtgt tttggttcag cgcgctggca
     6121 ttgtggattt atatcgccaa ggaaaatttc ttccgcccat tcggactgac ggcgaaattc
     6181 ggcaaaccgg attgggcggt gttcaaacag atttggaaaa tcggcgcacc catcgggctg
     6241 tcttattttt tggaagccag cgcgttttcg tttatcgtgt ttttgattgc gcctttcggc
     6301 gaggattatg tggcggcgca gcaggtcggc atcagtttgt cggggattct ctatatgatt
     6361 ccgcaaagcg tcggctcggc ggggacggtg cgcatcggct tttcgcttgg gcggcgcgaa
     6421 ttttcgcggg cgcgttatat ttcgggcgtg tcactggtgt caggatggat gctcgccgtg
     6481 attaccgtgc tttccttggt attattccgt tcgccgctgg taagtatgta caacaatgat
     6541 ccggcggttt taagcatcgc cgccaccgtc ttactgttcg ccggcttgtt ccaaccggca
     6601 gacttcaccc aatgtatcgc ctcctacgcc ttgcgcggct acaaagttac aaaggtgccg
     6661 atgttcatcc acgccgccgc cttttggggc tgcggtctgc tgccgggcta cctgctcgcc
     6721 taccgtttcg atatgggcat ttacggcttc tggacggcat tgattgcctc gctcaccatc
     6781 gccgccatcg ccttggtgtg gtgcttggaa ttgtgcagta gggagatggt cagatcgcat
     6841 aaggctgtct gaaaactccg ccgattaaaa atatagtgga ttaacaaaaa ccagtacggc
     6901 gttacctcgc cttgccgtac tatttgtact gtctgcggct tcgttgcctt gtcctgattt
     6961 ttgttaatcc actatagaaa cgatgggctg aagcccgcca ctggcaatac tcattttaaa
     7021 gttcaaacca tgcaacccat ccgataccga accgacctta ccccctacaa cactttcggc
     7081 ctgcgcgccc aagcccgagc ctttattgcg cttgaacacg ccgacgggtt gcgcgacatc
     7141 gtccggctac ccgagttcaa ccgagatact gttttatggc tgggcggcgg cagcaatatc
     7201 cttttgatgg aggactacgc cggtctggtc gtacatatgg aaaacaaagg catacgcgaa
     7261 attgctcgtt cagacggcat ggttctgatt gaagcgcagg cgggcgaaat ctggcacgat
     7321 tttgtcctgc acgccgtcgc gctgggtttg aacggtttgg aaaacctgag cctgattccg
     7381 ggtacggtcg gcgcgtcgcc cgtgcagaac atcggcgcat acggcgtaga ggcgaaagat
     7441 gtgattcaca gcgtgcgctg cttcgatttg gatacggaaa cctttgtaac cctttccaat
     7501 gccgactgcc gcttcgccta ccgcgaaagc ctgttcaagc aggaaggtaa agggcgttat
     7561 gtgattgttt cggtcgtatt tgcattaaaa acgcattttg tgccgaactt gggttacggc
     7621 gatttggcgg ccgccgttgc cgaactgagc gcggacaggg tgccgacggc gaaagatgtt
     7681 tccgatgcag tgtgtgcaat ccgcaacagt aaacttccta atcctaacgt gcttggcaat
     7741 gtcggcagtt tctttaaaaa ccccgtcgtc agcgcagaaa aagccgccac cttgttgcag
     7801 cggcatcctg atatgccgcg ctatccgcag cccgacggtt cggtcaaact cgccgccggc
     7861 tggctgatcg accaatgtcg tctgaaaggc tttcaaatcg gcggcgcggc ggtacatgac
     7921 aggcaggctt tggtcttagt gaacaaaaac aatgcctcgg caaacgatgt ccggcagttg
     7981 gcgcaacaca tcaaatttac agtatttgct cggtttcagg tagaattgca cgccgaaccc
     8041 aattggctgc ctgcttcgtt cagcctgtaa atccaaggag tatgcccgtg acccgccccg
     8101 ccaaaatcaa tacttacaca cgcatcatcg acgccagcct tgcgcttttc aacgaggaag
     8161 gcgagcgcaa catcagcacc aaccatattg ccgcccactt gggcatcagt ccgggcaacc
     8221 tctattacca cttccgcaac aaagacgaaa tcatcgtcca actgttcaaa cgttacagcg
     8281 aagccctgct ggaatacctc aacgaagccg tgttgccgtc tgatgtggaa gactccatca
     8341 attatatggc gggcatttac gacgtgatgt gggaataccg cttcctattc agcgacgtga
     8401 acaccctgct tgcacgcagt gccgaactat tgggcgaaca caataccttc acccaagcca
     8461 aagtctcccc gctcttggtc aacctgctga cccagctcaa cggtctgaac atcatccaag
     8521 ccgaccaaac cgccatgaac gacctcgccg tcaatatgtg gatgatcacg aaatactggt
     8581 tcgacttcga cagctccctg cgcggccgca ccaagctgac cgaagactcc aaagcacgcg
     8641 gcatccgccg caccttaagc ctccttcgtc cttatctttt gcccgaacac cgcgagaaat
     8701 acgaccggaa aatcggcaac ggcaacccgt aaacccacag cgtccgaatc agccttcaga
     8761 cggcacttac ccgacggtat cagcgaatgc gcgcagtccc cccgccccat atctccatca
     8821 caggcctagg ttacctcggc ctgccgctgg cacaaaagtt ttaccaacac ggcagccgcg
     8881 tcgccgccat aaaacgcagc ctgacttcgg acgacatcaa cctgcccata catctcgaca
     8941 ccttcgacct cggcagcacc gatgcctttc agacggcatt gtggcggcac cacgccgaca
     9001 aacccgtctg gttcttcctg ctaccgccct cctcgctgac gcattacgcc gacaccgtca
     9061 aacaatgggc agaacttgcc cgggcgtgca acgtgcaaca cctgattttc acaagcagca
     9121 ccagcgttta cggcgataca gcgcgcgaat gcgacgaaac cgccacaccc gatccgcaaa
     9181 ccgagtccgc ccgccaaatc ctcgccgccg aacaacacct gctcgacagc ggcatctcca
     9241 acatcgacat cctgcggctg ggcgggcttt attgcgccga acgccatccc gtcagccgcc
     9301 ttgttcaaaa gcaaaacatc ccgggcggca accgccccat caacatcgtc caccgtaata
     9361 tcgccgtcga aagcctgttt cagacggcat ttaaccccgg cggcaggcgg ctgaaaaaca
     9421 ttatcgaacc gcgccacccg acacgacgcg aattctatac ggaagaagcc gccaaactcg
     9481 gcttgccggc tcctgatttc gcacccgacg acagcgtggg caaaatcatc cgtaccgttt
     9541 gcgataacgg cttaagcctg taaaataagc gtcaacagca acaaacaggc attctccgcc
     9601 ccgatcagaa aatatgtccg ccctcctccc catcatcaac cgcctgattc tgcaaagccc
     9661 ggacagccgc tcggaacttg ccgccttcgc aggcaaaaca ctgaccctga acattgccgg
     9721 gttgaaactg gcgggacgca tcacggaaga cggtttgctc tcggcgggaa acggctttgc
     9781 agacaccgaa atcaccttcc gcaacagcgc ggtacagaaa atcctccaag gcggcgaacc
     9841 cggggcgggc gacatcgggc tcgaaggcga cctcatcctc ggcatcgcgg tactgtccct
     9901 gctcggcagc ctgcgttccc gcgcatcgga cgaattggca cggattttcg gcacgcaggc
     9961 agacatcggc agccgtgccg ccgacatcgg acacggcatc aaacaaatcg gcaggaacat
    10021 cgccgaacaa atcggcagat tttcccgcga acccgagtcc gcaaacatcg gcaacgaagc
    10081 ccttgccgac tgcctcgacg aaataagcag actgcgcgac ggcgtggaac gcctcaacga
    10141 acgcctcgac cggctcgaac gcgacatttg gatagactaa ccttcagacg gcatccgaca
    10201 tgaacagccc ttttcacaac atcggcatcg taacccgccc caacacgccc gacatccaag
    10261 acaccgcaca cacgctgatt acctttttga agcagcacgg ctttaccgtc tatctcgacg
    10321 aagtcggcat aaaggaaggc tgcatctata cccaagacac cgtcggctgc catatcgtca
    10381 acaagaccga actggggcaa tactgcgacc tggtcgccgt tttaggcgga gacggcacct
    10441 ttctctccgt cgcccgcgaa atcgccctgc gcgccgttcc gattatcggc atcaaccaag
    10501 ggcatttggg cttcctgacc caaattcccc gcgaatatat gacggacaag ctattgcccg
    10561 ttttagaagg gaaatacctt gccgaagagc gcatcctgat tgaggccgca ctcatccgcg
    10621 aaggcaaaac cgccgaacgc gccatcgccc tcaacgatgc cgtcctctcc cgtggcggcg
    10681 ccggacagat gattgagttt gaagtcttcg tcaatcggga attcgtctat acccagcgtt
    10741 cggacgggct gattgtctcc acccccaccg gatcgaccgc ctattcgctt gccgccggcg
    10801 gccccatcat gcaggcagga ttgcacgcct tcacgctcgt ccccatctgc ccgcaatcca
    10861 tgaccaaccg ccccatcgcc attccagaca cgtccgaaat cgaaatcctc gttacccaag
    10921 gcggcgacgc gcgcgtccat ttcgacggtc aaacccatat cgacgtgcaa aacctcgacc
    10981 gcatcaccat ccgccgctac cgaaacccct tacgcatcct acatccgacc gactaccaat
    11041 atttcaaaac cctgcgccaa aaactgcact ggggcgaaca attggtctga accgaccctt
    11101 accggaaaac acctatgaat ccaaaaactc cccccgacca tacagaagat gccgtgcgcc
    11161 tctccaaacg catggcgcaa ctggggcttt gttcgcgccg cgaagccgac ggctacatcg
    11221 aacaaggctg ggtaacggtc aacggcaaaa ccgccgtact cggtcagaaa gtttcaccgg
    11281 ccgaccgtat cgaactaaac aagaaagccc acgaacagca ggcggcacgc attaccatcc
    11341 tgctgaacaa acctgtcggc tatgtcagcg cacaagcgga aaaaggctat aaatccgccg
    11401 ccgaactgat tacccctgaa aaccgctggg aaggcgacac cggacgcatc cgtttcgatc
    11461 cgaaacacaa aatcggcctc gcccccgccg gcaggctgga catcgactcg gtcggattgc
    11521 tggtattgac gcaggacggc cgtatcgcca agcagcttat cggcgaaaac agcggcagtg
    11581 aaaaagaata tttggtgcgc gtgcgcggca aattggacga aaaaggactt gccttactga
    11641 atcacggatt gagtttggac ggcgagaaac tgcgtcccgc ccaagtagaa tggcaaaacg
    11701 aagaccaact gcgcttcgtg ttgaaacagg gtaaaaagcg gcaaatccgc cgtatgtgcg
    11761 aactggtcgg actgcgcgtc gtcgggctga aacgcatccg catgggcaag gtcaaactcg
    11821 gcaggctgcc gcccggcaaa tggcgttatc tcgcccccgg tgaatcgttt taaataagca
    11881 ttaaaaaatg ccgtctgaaa atccttgcgt tttcagacgg cattgcctta tgcccaaata
    11941 acggtttctt ccaagggcct gcgcgccttc gtggcgattt cctgaacgcg gtaaccgaat
    12001 gtcgcggcga cggacacgcc ccattctgcc gcatcgaaca aaccgaactg cccggacaat
    12061 acgcgttcca tatcggcata gttgaaacct tccaccgggc aggaatcgat acctgccatc
    12121 gccgcacccg tcatcatgtt gcccaacgcg atataggtct ggcggcaaca ccagtcaaac
    12181 aaggcgcgcg aatcgtccaa aatcttgatg tcgtcggctt gaaacgcctg atacctcgcc
    12241 aaagattttg ccatggcatc cggttcggta acgccgcgcc gtttgaggct ttccaacata
    12301 aacgggctgt cgaagcgggc atttttcttt gccaaaaaca ccaccaaatg actggcggta
    12361 tccaaagcat ccgccatacc ccaagaaaac ggcttgattg cctgtcggat ttcggggttt
    12421 tgaaccacaa caaactgcca aggctccgaa ccgaccgaac tgggcgacaa acgcccgagt
    12481 tctaaaataa actgaaaatc ctcggcactg attttacgtg ccgcatcgta atgccggcat
    12541 gatttgcggt ttttaaatgc ggatagaacc tgctctctgt ctaataccgt catagtgcat
    12601 cctttcatta cttgtattgc aaattgattt caaaccgata cggcactgcc gcaccataca
    12661 atccgtcctc gccatcaagc ttcatccgga ctttacccat ctctctacgg ctgggaaaac
    12721 aaagcggcgg cagactcggg aataacgccc caatgccttt cagacggcat ttgggcatca
    12781 ttgcaaacgc gcctacgggt aggcgatata ggcataagcc gaatggttgt ggatagactc
    12841 gaaattctcg ctctcgacga cgaaactctt gatgcgtttg tcggcaatca gcgaagtagc
    12901 gacatcgcgc accatatctt ccacgaattt cgggttttcg taggcttttt cggtaacgta
    12961 tttttcatcg gggcgtttga gcaggccgta gagttggcag ctcgcctgcg cctccacata
    13021 atcgatgact tcctcgatac cgacttcggc atcggcagtc aggctgacgg taacgtgcga
    13081 acgctggttg tgcgcgccgt attgggaaat ttctttggaa cacgggcaaa gcgaggttac
    13141 gggaatcatg accttcatac tgtggccgta tgccccgtct ttgatttcgc ccgtgagaca
    13201 gacatcataa tccagtaagg accgaatacc ggaaaccggc gcggttttct tgcggaaaaa
    13261 cggaaaagaa acgctgattt tgccggcgcg ggaatctaaa agcgcgacca tttcggtagt
    13321 cagcttgcgc aattgtgcaa aatccaaggc ttcggcatgt tgctccatca atgcgacaaa
    13381 acgcgacata tgcgtcccct tctgctcagc aggcaggtaa accgtcatgg tcagacgggc
    13441 aatggtggac tggatgcctt ctgcagtttg cagggtaatc ggaaagcgca ggtctttgat
    13501 accgacctga ttgatcggca gattgcgcaa atctcggctg gattgcacgt ctgcaatagt
    13561 gttcatgagt tgtttttcct taatattcat gtaattgttg agatgtgtgc atggtatggc
    13621 gtttccgccg gggaatatac cgtcaatctg caaatgcgag attatatcac tcgcttttag
    13681 cagctgcatt gattgtttct atttttcaga tagtgataaa atcttcgatt attcacatct
    13741 acataggcag agaccaaatg aaattcgcca cgaaagccat tcattccagc tacgattgcg
    13801 acgaacacaa ccgcgcgctg atgccgccga tttatcaaaa cagtatgttt gcgttgcacg
    13861 agattggcga aaatgtgcct taccgttatt cgcgcctgag caacccgacc cgtcagattt
    13921 tagaagacac cgttgccgat ttggaacacg gtgcggcagg ttttgcgttt tccagcggta
    13981 tggcgggaat tgatgccgta tggcgcactt tcctgcgccc gggcgatacc attgtcgccg
    14041 tcgccgatat ttacggcggc gcttatgatt tattggtcga tgtttatcaa aaatgggggg
    14101 tgaacgttgt ttttgccgat ttaggcaatc cggataattt ggacgaactg cttaaagcgc
    14161 acaaggtcaa actggtttgg ctggaaacgc cgtccaatcc acttttacgc ttggtagaca
    14221 tcaaagtcct tgccgcaaaa gccaaagcag ccggtgcgct ggtcggtatc gacaacactt
    14281 ttgccacgcc gtatctgcaa cagccgttgg atatgggttg cgattttgta ttccattccg
    14341 ctaccaaata tttgtgcggc cattccgacg tgttgatggg catcgtcgtt gccaaaacca
    14401 aagaactggc gcagcctttg cacgatatga tggtgcatac cggcgcggtt gccggcccgc
    14461 tggactgctg gctggtgttg cgcggcatca aaacactggc tctgcgcatg aacgcccatt
    14521 gccaaaacgc actcgaaatc gcgcgccgtt tggaagccca tcctgccatt gaaaaagtgt
    14581 tccatcccgg cctgccgtct cacgaacatt acgaactggc gaaaacacaa atgcccaaag
    14641 gcatcggcgg cgtggttacg gtttatctca aaaacgacac gcgtgaagcg gcaaacagcg
    14701 tgattaaaaa catgaaactg gtcaaaatgg cttccagcct cggcggtgtg gaaagtttgg
    14761 tcaaccattg ctattcccag tcccacagcg gcgtaccgca tgatgtgaaa atggaaatgg
    14821 gcatcaaagt cggtctgctg cgtttctcca tcggcatcga agacgcggac gatatttgga
    14881 acgatatttc cgccgcactc gatacaactt tgtaaactgt aaaaatgccg tctgaaacca
    14941 tggtttcaga cggcatttca attaacccgg ccgaaaatca acgcttcaac atctttgccg
    15001 cctcaatagc gtaataggtc aaaatcccgt ccgcacccgc acgtttgaat gccagcaggc
    15061 tttccaaaac cactttgccg ccgtccagcc agccgttggc aatcgctgcc tgcaacatcg
    15121 cgtattctcc cgaaacctga taggcataag tcggcacacc gaactcgtcc tttacgcggc
    15181 ggacaacgtc caaatacggc aaaccgggct tgaccattac catatccgca ccttcctgaa
    15241 tgtccaacgc cacttcgtgc aacgcctcat cggtatttgc cggatccatc tggtaggtct
    15301 ttttatctgc cttgcccaaa ttgcccgaac tgcctaccgc atcacggaaa gggccgtaaa
    15361 atgcagaagc atatttggcg gaatacgcca taatccgcgt atggatatgc ccggcatcct
    15421 ccaacgcctc gcgaatcgca ccgatacgcc cgtccatcat atcggaaggg gcaaccacct
    15481 gcgcgcccgc ttcagcgtgg cacaaagcct gcttgaccaa aacctctacg gtttcatcgt
    15541 tcatcacata accgttttcg tccgtcagcc cgtcctgacc gtgaaccgta taaggatcga
    15601 gcgcgacatc cgtcataatg cccagttcgg gaaacctctc gcgcaaggcg cggacagttg
    15661 acggcacgag tccttcggga ttgtacgcct cctgcgcacg ctcggttttg tttgccgtaa
    15721 ccacggggaa cagtgccaac atcggaatac cgagctttac cgcctcttcc gccgtaaaca
    15781 gcagcctgtc caaactttga cgcttcacac ccggcataga aggcacatcc tcctcgcgcg
    15841 ccgacccctc caatacgaac accggataaa tcaaatcatc ggcggtcagc gtgtgttcgc
    15901 gcatcaggcg gcgtgaaaaa tcgtccctgc gcatacggcg catacgcgaa gccggaacat
    15961 tgcggtaagg aaactgcata agccctccaa tcatcctgtc aacaattcaa accatacgga
    16021 agccgccgca acttgcagac agcatcgggc ggcaatcaaa atccgttttc accggaaagg
    16081 gtttcggccc gccgaaatgc cgcgccgaag gacggcgtcc cgcccattaa agaacaatag
    16141 caaaacaaca gcttcaggct gtcagccttc gatattctta cgccacaata ccaaaagttt
    16201 cccgatatgc tgaacaggtt gcgcgtcaac cgcctcacat aaggcagtgc agatttcgat
    16261 acgttcggca cggtcgtcgc cgaatacgcg cactttaatc agctcatgcg ccgtcaatgc
    16321 cgcatcggtt tccttgatga ccgcgtccgt cagaccctgc tgaccgacca tcacaacagg
    16381 atggagatgg tgcgcgcgcg ctttcagttc caaaatttct ttggtgttca atttggtatc
    16441 cgtcattttc ctactgcttg aataaaaaga ataatgcggc attgtacgcg atttggcacg
    16501 acatcggcaa aattaacgta caatacgcgg tttccattcc gacccgcgaa ccactatggc
    16561 tgtacgttcc aaatcctcaa aagcgtggct gcacgaacac gtcaacgacc actacgtcca
    16621 tatggcgcaa aaagacggct accgcgcccg tgccgcatac aaacttttgg aaatcaacga
    16681 aaaagacaaa ttaatcaaac ccggcacggt acttgccgac ttgggcagcg caccgggaag
    16741 ctggtcgcag gttgccgcca agctgacggg tacttccgga gcagttttcg ccttggacat
    16801 cctgcctatg gaagccatag ggggcgtctc cttcattcag ggcgacttcc gcgagaacga
    16861 cgtactggca caattcgaaa ccttgttgga caaccgcccg ctcgaccttg taatttgcga
    16921 tatggcgccc aatatgtcgg gaaacgccgt aagcgatcag gcacgcagct tttatctgtg
    16981 cgaactggct ttagacttcg cctcgcaaca cctgaaaacc ggcggcagct ttttggtcaa
    17041 agtctttcag ggtgcaggct atcaggaata catggcagcc atgcgcgaaa ttttcggcac
    17101 ggtgcagacg cgcaaacccg aagcctcgcg caatcgctcc agtgagattt atttattggg
    17161 caaaaataaa cgctgacaat acagacggcg tgctttacaa tcatttccgt tttacacctc
    17221 aattatggag ccttgctaag tggggaacac ctttaaatca atccttgtct gggtcgcctt
    17281 gggtatcggc ctgatggctg cgttcaacgc tttagacggt aaaaaagaag acaacgggca
    17341 aatcgaatat tctcagttca tccaacaggt caacaacggc gaagtatccg gcgtcaacat
    17401 cgaaggatcc gtcgtcagcg gctacctgat taagggcgag cgcaccgaca aaagcacctt
    17461 cttcaccaac gcgcctttgg acgacaacct gattaaaaca ctgctcgaca aaaacgtccg
    17521 tgtaaaagta acgccggaag aaaaaccgag cgcgctggct gccctgtttt acagcctgct
    17581 gcccgtcctg ctgctgattg gcgcgtggtt ctactttatg cgtatgcaga cgggcggcgg
    17641 cggaaaaggc ggcgcattct cattcggcaa aagccgcgcc cgcctactgg acaaagatgc
    17701 caacaaagtt acctttgccg atgtcgccgg ctgcgacgaa gccaaagaag aagtgcagga
    17761 aatcgtcgat tacctcaaag cgccgaaccg ctatcaaagc ctgggcgggc gcgtgccgcg
    17821 cggcatcctg ctggcgggca gcccgggtac gggtaagacg cttttggcga aagcgattgc
    17881 aggcgaagcc ggcgtgccgt tcttcagcat ttcaggttcc gactttgtcg aaatgttcgt
    17941 cggtgtcggt gcaagccgcg tccgcgatat gttcgagcag gcgaagaaaa acgccccctg
    18001 catcatcttt atcgacgaga ttgacgcagt cggccgccaa cgcggcgcag gtttgggcgg
    18061 cggtaatgat gagcgcgagc aaacattaaa ccaattgttg gttgaaatgg acggttttga
    18121 gagcaatcag actgtaattg tgattgcggc aaccaaccgc cccgacgtac tcgatcctgc
    18181 gctgcaacgc cccggccgtt tcgaccgcca agtggttgtc cccctgccgg acatccgggg
    18241 gcgcgaacag attttgaacg tccactctaa aaaagtgcct ttggacaaat ctgtggattt
    18301 attgtccctc gcgcgcggca cgccgggttt ttccggcgcg gatttggcga acttggtcaa
    18361 cgaagccgcc ctgtttgccg gccgccgcaa taaagtcaaa gtcgatcaga gcgattttga
    18421 agacgccaaa gacaaaatct atatgggtcc ggaacgccgc agtatggtga tgcacgaaga
    18481 cgaaaaacgt gcgacggcgt atcacgaatc cggacacgcg attgttgccg aaagcctgcc
    18541 ctttaccgac cccgtccaca aagtaaccat tatgccgcgc ggacgtgcgc tgggtctgac
    18601 ttggcagctt ccggagcgcg accgcatcag tatgtataaa gatcagatgt tgagccagct
    18661 ctccatcctg ttcggcggac ggattgccga agacatcttc gtcggacgca tctccaccgg
    18721 cgcatcaaac gactttgaac gcgcaaccca aatggcgcgc gaaatggtaa cgcgctacgg
    18781 catgagcgac aaaatgggcg tgatggttta tgcggaaaac gaaggcgaag tcttcttggg
    18841 acgcagcgta acccgttcgc aaaacatttc cgagaaaacc cagcaggaca tcgacgcgga
    18901 aatccgccgg attttggacg agcaatatca ggttgcctac aaaatcctcg atgaaaaccg
    18961 cgacaagatg gaaacgatgt gcaaagccct gatggaatgg gaaaccatcg accgcgatca
    19021 ggtactggaa atcatggcgg gcaaacaacc cagcccgccc aaggattaca gccacaacct
    19081 gcgcgagaat gcggacgcgg cggaagataa cgcgccgcac gctccgactc gggaagaaac
    19141 cgaagcacct gccccggcag acaccgcttc gtcagagtcc gagcagcggc ctgaaaacaa
    19201 ggcttaactt cccgaacaaa cggcagcccg caagctgccg ttttcccatc ccgttttcag
    19261 acggcatttt ccactcaata ccgtctgaaa cgccaaagcc gtatcggttt ttacgaatct
    19321 gcgatatggc ggtttaaatt gaaatcagaa cggggcaagg ctgtaccggt ttaaaattaa
    19381 accgctataa taccgccctt cgaatacacg cccgcattgg tgatgcccgt gaaaaaagtt
    19441 gaaaaaaaca tcttggtgct gcacggcgcg gacaaaatgt tcgagctggt cgataaggtt
    19501 gaggattacc cgaactttct gccgtggtac agcaaaaccg aggtcatcgg gcgcaacggc
    19561 aacgaactga aggcgcggct gtttatggac tatatgcacg ttcgccaatc gtttgccacg
    19621 cacaaccgca acatccccgg cagggaaatc cgtatggaac tgctcgaagg cccgttcaaa
    19681 accttacgtg gaacgtggaa atttatcgat ttgggcgacg atatgtgcaa aatcgaattc
    19741 aaccttgaat acgatttttc taatgccgtc ttatccgcct taatttcccc cgtcttcagc
    19801 cacctttccg ccacgctggt cgaagcgttc gtcaaagagg cagaccgccg ttatgcttga
    19861 aattgagatt gtgtacggac tgcccgaccg acaggttttg aaaaccatgc ggcttgccga
    19921 gggaacaacc gtccgcgccg ccgcactgca aagcggtttg gacggcatat ttgaagattt
    19981 aaacctgcat tccgcgcctt tgggcatttt cggcaaggcc gtcaaagacg acacgcccct
    20041 gcgcgacggc gaccgcatcg aagtgtaccg cccgctgttg atcgacccca aagaagcgcg
    20101 ccgcaaacgc gttcaaaatc aagaagaata accatgccgt ctgaagcctt cagacagcat
    20161 tcgacagaaa cacggaaaat atcatgtcaa acaaaatcaa aatggttgtc ggcttgggca
    20221 accccggcaa agaatacgaa caaacccgac acaatgcggg tttttggttc ctcgacgaac
    20281 tggcgtggaa atggaaggct tcatttaaag aagaaaaaaa attcttcggc gaagtcgccc
    20341 gtgccgccct gcccgacggc gatgtctggc tgctcaagcc gaccacgttc atgaaccgtt
    20401 ccggacaggc agttgccgcg cttgcacagt tctacaaaat caaacccgaa gaaatcctcg
    20461 tcgtccacga cgaactcgac attccctgcg gacggatcaa attcaaactc ggcggcggca
    20521 acggcggaca caacggcttg aaagacattc aggcaaaact cggcacggca gactattacc
    20581 gcctgcgcct cggcatcggc cacccgggcg accgcaacct cgtcgtcggc tatgtcctga
    20641 acaaacccag tacggaacac cgccgacaga ttgacgatgc cgtcgccaaa tccctgcagg
    20701 ccatacccga catccttgcc ggcaaatggg aagaagcaac ccgcttcctg cacagcaaat
    20761 gacccgatgc cgtctgaagc cctttcagac ggcattttcc cgatttccat atccgaacag
    20821 tcatgaccga actcaagcag cttatccaaa ccgaatccat ccccgtcatc gaagaaaccc
    20881 tcgatttcct gctgtacgaa tgcagcatcg acgacgcacc gtccgccgaa gaagtggcac
    20941 aatggcgcga catacttgcc gcacgcggcg gcaaattcct gcgcctgtcc aaaatctgcc
    21001 aaacgtggct ggacgaggag gcggcatgaa gctgccgcgc aaccgcttca gcctgctttc
    21061 cgcattgtgg tttgccggcg gcatctattc gctgctcttc aaagctgccg acaccgcgcc
    21121 gccgccgttt ccgcatttcg acaaagcagc acaccttgcc ctgtttttcg cacaaatctg
    21181 gcttttgacc aaagcattca aaaccggaaa acttcccatc ccctaccgca gcctgatggt
    21241 ctttgccctc tgtttcgccc tcttcagcga atgcgcgcag gcatggttta ccgcaacgag
    21301 aaccggcagt ttgggcgatg tccttgccga cctgacgggc gcagcccttg ccctctttac
    21361 cgcgcgagct gcctgccgcc cggactaaat cggtttattt tccccaaaca ggatgcacct
    21421 cttcccgata tatcctattt tccccttaca tatcaatccc atctcataaa caaaaccgac
    21481 aaatcgttta caatatattt acactacatc agattacaaa tatactcgaa ccagttgcag
    21541 aagcggcgat tccacaaacc gtttcggatt gcacgacaca aaagaaaaac cgattttgtt
    21601 gccttaaagg agtattcatg aacctgcatg caaaggacaa aacccagcat cccgaaaacg
    21661 tcgagctgct cagtgcgcag aagccgatta ccgactttaa gggcctgctg accaccatta
    21721 tttccgccgt cgtctgtttc ggcatttacc acatcctgcc ttacagcccc gatgccaata
    21781 aaggtatcgc gctgctgatt ttcgttgccg cactttggtt taccgaggcc gtccacatta
    21841 ccgtaaccgc actgatggtg ccgattctcg ccgtcgtact cggtttcccc gacatggaca
    21901 tcaaaaaggc gatggctgat ttttccaacc cgattatcta catttttttc ggcggcttcg
    21961 cgcttgccac cgccctgcat atgcagcggc tggaccgtaa aatcgccgtc agcctgttgc
    22021 gcctgtcgcg cggcaatatg aaagtggcgg ttttgatgtt gttcctcgtt accgcctttc
    22081 tgtccatgtg gatcagcaac accgccaccg ccgcgatgat gctgcctcta gcaatgggta
    22141 tgctgagcca cctcgaccag gaaaaagaac acaaaaccta cgtcttcctc ctgctcggca
    22201 tcgcctattg cgccagcatc ggcggcttgg gcacgctcgt cggctcgccg cccaacctga
    22261 ttgccgccaa agccctaaat ctggacttcg tcggctggat gaagctcggc ctgccgatga
    22321 tgctgttgat tctgcccttg atgctgctct ccctgtacgt catcctcaaa cctaatttga
    22381 acgaacgcgt ggaaatcaaa gccgaatcca tcccttggac gctgcaccgc gtgatcgcgc
    22441 tgttgatttt ccttgccaca gccgccgcgt ggatattcag ctccaaaatc aaaaccgcct
    22501 tcggcatttc caatcccgac accgttatcg ccctgagtgc cgccgtcgcc gtcgtcgtct
    22561 tcggcgtggc gcaatggaag gaagtcgccc gcaataccga ctggggcgtg ttgatgctct
    22621 tcggcggcgg catcagcctg agcacgctgt tgaaaacatc cggcgcgtcc gaagccttgg
    22681 gacagcaggt tgccgccacc ttttccggcg cgcccgcatt tttggtgata ctcatcgtcg
    22741 ccgccttcat tatttttctg accgagttca ccagcaacac cgcctccgcc gcattgcttg
    22801 taccgatttt ctccggcatc gctatgcaga tggggctgcc cgaacaagtc ttggtattcg
    22861 tcatcggcat cggcgcatct tgtgccttca tgctgccggt tgccacaccg cctaacgcga
    22921 ttgtgttcgg cacgggctta atcaagcaac gcgaaatgat gaatgtcggc atactgctga
    22981 acatcctctg cgtagtattg gttgctctgt gggcttatgc tgtactgatg taaaccatcg
    23041 acctaaacaa caagaccgtc tgaaagaata tttttcagac gaccttgaag tttgtcgttc
    23101 agacacaatt tgtcgaatca ttcaaaaccg gattctaacg aaaggaaacc catgattatc
    23161 ctgcacacca acaaaggcga catcaaaatc gaactcgatt tcgacaaagc ccccgttacc
    23221 gccaaaaact tcgagcaata cgtcaaagac ggcttctacg acggcgtaat cttccaccgc
    23281 gtcatcaaag gcttcatgat tcaaggcggc ggcatggatg aaaacatgaa cgaaaaagaa
    23341 acccgcgatc cgattcaaaa cgaagcgtcc aacggcctgc ccaacgataa atacaccatc
    23401 gccatggcac gtacttccga cccccattcc gccggcgcgc aattcttcat caacaccgcc
    23461 gacaatgcct tcctcaacca ccgctccaaa gaaatgtacg gcagaaccgt cgtccaagac
    23521 tggggttatg ccgtattcgg caaggtagtc ggcggttttg acgtggtcga tgccatcgaa
    23581 ggcgtatcca ccaaacgcca cggctaccat gatgatgtgc cgacagaacc tgtcatcatc
    23641 actaaagccg aagcggtata aaccggcaat ccgaaagcag cctgcacaaa ggctgctttt
    23701 tttgtttcag gcggcataaa atccaagcac atatgccgtc tgaacacctc ccgcaagcct
    23761 tcggacaatg ttgcaacgtg ttaaaatagc ctcctttcct gaaaccacaa caaggaaacc
    23821 atcatggcaa atattgcccg cgacatcttc aaagcctacg acatccgagg cattgtcggc
    23881 aaaaccctga ctgacgatgc cgcctatctc atcggcaggg ccatcgccgc caaagccgcc
    23941 gaaaaaggca tgacccgcat cgcgctcgga cgcgacggac gcttgagcgg tcccgaactg
    24001 atggagcaca tccaacgcgg cctgaccgac agcggtatcg gcgtcctcaa tgtcggcatg
    24061 gttgccacgc ccatgctcta ttttgccgcc atcaacgaat gcggcggcag cggcgtgatg
    24121 attaccggca gccacaatcc gcctgattac aacggtttca aaatgatgct cggcggcgac
    24181 acgcttgccg gcgaagccat ccaagagctt ttgtccatca ttgaaaaaga cggttttgct
    24241 gccgccggca aacaaggcag cgtaaccgaa aaagacatct ccggcgaata ccacaaccac
    24301 atcgtcggac acgtcaaact caaacgcccg atgaacatcg ctatcgacgc gggcaacggc
    24361 gtgggcggcg catttgcagg caaactctac cgcgctttga ggtgcggcgt taccgaactt
    24421 ttctgcgacg tggacggcaa tttccccaac caccaccctg atccttccaa accgaaaaac
    24481 ctgcaagatt tgattgccgc gctgaaaaac agcgatgccg aaatcggctt ggcttttgac
    24541 ggcgatgccg accgtttggg cgtggttacc aaagacggca acatcatcta tcccgaccgc
    24601 caattaatgc tgttcgccca agacgttttg aaccgcaatc ctaaagcaaa agtgattttc
    24661 gatgtcaaat ccacccgcct gctgactccg tggattaaag aacacggcgg cgaacctgtg
    24721 atggaaaaaa ccggccacag cttcatcaaa tccgccatga aaaaaaccgg tgcgctggtt
    24781 gccggcgaaa tgagcggaca catcttcttt aaagaacgct ggttcggctt cgacgacggt
    24841 atgtatgccg gcgcgcgcct cttggaaatc ctgtctgcct tcgataatcc gtccgaagtg
    24901 ttgaacaagc tgccgcaaaa catttccacg cccgaactta acatcgacct gcccgaaggc
    24961 agcaacggcc acaaagtgat tgaagagctg actgccaatg ccaagtttga aggtgcgacc
    25021 gaagtcatca ccatcgacgg cttgcgtgtt gaattccctg acggcttcgg cctgatgcgc
    25081 gcttccaata ccacgccgat tttggtattg cgttttgagg cggatacgca agaagccatc
    25141 gagcggattc aaaaccaatt caaagccgtc atcgaaagca atccgaatct aatctggcct
    25201 ctgtaaacac aggaaaaatg cttaatcaaa tcttggtaga ttggatttgg cttaaaaatc
    25261 tccgcgtccg ttcgtgtttg gggtggtcga acaactcttt cgggctgccc tgctctacga
    25321 taacgccgcc gtccatcacg acaacggtcg tggcaacttc cagcgcgaac ttgatttcgt
    25381 gggtaacgac gaccatcgtc caaccttccc gcgccaattc cttcatggcg ttcaacacgt
    25441 cttgcaccaa ctcggggtca agcgcggaag tgggttcgtc aaacaacatc agctcgggct
    25501 gaatcgccaa tgcgcgggca atgccgacgc gctgctgctg accgccggaa agctggtagg
    25561 gatacaaatc cactttgtcg cccaagccga ctttttccag cagtttcaga gcctcttcgc
    25621 gcgcttgggc ggcaggcttg ccctgtacgg caaccggtcc ttccatcacg ttttccaagg
    25681 cggttttgtg cggaaagagg ttgtattgtt gaaacaccat gcctgatttg cggcgcagtg
    25741 ccaaaatatc gtgtttgctt ggttttttag aaaaatcgat tttcagcggt cgctcgttgt
    25801 cgaactcgat ttgtccgtct tcgggcattt ccaacgcgtt taggcatcgc agaaacgtcg
    25861 ttttgcctga gccggaaggc ccgaggatga cgaccacctg ccctttgcac acatccaaat
    25921 tgatgccgcg caaaatggta tttttgccga aggtcttatg gatattgcgg attttaatca
    25981 tgacaactcc ttatttggcg acgtagcggt cgaaacgttt ttccaaacgc gcctgaatca
    26041 ggaacagcac tttacaaaaa caccagtaaa ccaaagcggc ttcgatatag acgggcaaaa
    26101 agtcataagt gcggtttgcc gtttcctgcg cgacgcggaa taattccgtt accgtcacga
    26161 ctgccgcgag cgaggtgttt ttaaacaaac cgataaactc gttgctcaaa ggcggcacgg
    26221 caacgcgaaa tgcctgcggc gcgacgatgc ggcggaacgt ctgcatatag gtcatgccga
    26281 tggagaaacc tgcttcccat tggcctttcg gtacggacaa aattgccgcg cgtatggttt
    26341 cggaagcata tgcgccgaca ttgagcgaaa agccgatgat ggcggcagga atcgggtcga
    26401 tatagatgcc gacggaaggc agcccgtaaa acacaatcac aagctgaacc aacagcggcg
    26461 taccgcgaat gacggaaata taaaattcca ccaatttcag caggattttc cgcacgatgc
    26521 cgccggcggg cataatccgc accaaagcca cggctaccgc aatcatcata ccgataacga
    26581 aagaagctgc cgccaaaggc agagagaccg cgaagccggc tttgaccata ggcaaaaacg
    26641 cgctgacaat catatcggcg cgtgtttccg tcataaacgg cagcgaagca aggaaattat
    26701 tgaacactga tgtcttttcc gaagaattgt tcgcccagtt ttttcagcgt accgtcggct
    26761 ttcagctcgt tgattgccgt actgaatttc gccacggctt cgtcattgcc cttgttgaca
    26821 atcaggccgg aaccgacttt ttcatcggca ggtgcggacc aaacgatttt cacgcccgca
    26881 ttcgggtttt tcttcagata gtccaaaacc gccaattcgt cgttcagggt tgcatcggca
    26941 cgtttttgtt caatcagggt cagcgattgc gccaaaccgt ccactgccac caaatctgcg
    27001 cctgcagctt tggctttttc gccgtagttg ctggtcaggg attgtgcggt tttcacgcct
    27061 ttgatgtcgt cgatagattt gatgttgctg tcgttacggg caaccaatac ggcaccgctc
    27121 cagctgtaag gatcggattt gtcgaatgtc gcttggcgtt cggggctggt cagaccgact
    27181 tggtttgcca ccacgtcgaa acgccccgcc ttcaaacccg ccatcatcga atcccattgc
    27241 gtttctttaa actcgacttt cacgcccagt ttttccgcca cggcgcgggt tacttccaca
    27301 tcgtaaccgg tcagtttgcc gtccttgtcg tggtaggtaa acggtgcgta agtgccttcc
    27361 gtgccgacgg taaccgtgcc tttattgttg atgcgctcga ttaaagaacc ggaaactgcc
    27421 gattgtgcag gcgcggaaga tgctccgctg ccgctttccg aaccgccgca ggccgccaaa
    27481 accaatgcgg caatgccgcc gagtacgaat tttttcaaca tcattctctc ctgtaaaaag
    27541 tgtgcaagtg cggcattcta agtgaaattg cggcaaacac aaagaatact ttttttatat
    27601 ggttattcca aatcggaatg gaaaagatat gccgaagccg ccggaagcgg aaataaaggg
    27661 ggcgggaaag gcggcgggcg gcacgaaacc gaaaagcggc atcaggtatg caaacggcgg
    27721 attcacgcac gccgttccgg caaaaatccg cctgccgatt cggtgctata atccatccga
    27781 aacagatgac accaaaaagg aacatctcat gaacacgccc caatccgcca tcattcccga
    27841 ccacgcccaa gccggcatct ttatcgaagc cgacttcgcc gccaaccgcc tcaacgatat
    27901 taaagccgcc tgccgcgctt cgctcgacgc gttatccgcc ttgaaaaccc gctttccgga
    27961 cgatattttg ggtctgacca tcgccttcgg cagcaaagcc tgggcaacat tcggacatac
    28021 ggacgaaggc agtgaaatca aacccttctc cgaaatgggc aacgggcttg cgccgtccac
    28081 gcagcacgat atgtccatcc acatccaatc tttccgccaa aacgccgcct acgcgcttgc
    28141 ccaatccgtt ttgggcgcgt tcggcgacag catatgcgtc gcgtccgaag aacacggttt
    28201 gcgcctgtat caggatcgcg ggctggacgg tttcgtcgac ggcacggaaa acccgcaggg
    28261 cgatgaaaat gtccgcgaag tcgccatcat ccccgaagga aaacccgatg cgggcggcag
    28321 ctatgtcctg ctgcaaaaat acctgcacga tttgaaaaaa tgggatgccg tccccgtcgc
    28381 cgaacaggag gcctcggtcg gacgcggcaa ggaaaccgac gacgaattcg gccacgatgt
    28441 ccgcctgccc gattcgcacc tcggccgcgt caacctgaaa gaaaacggag tcggcctgaa
    28501 aatcgtccgc cgcagcctgc ccttcggcaa aatcagcggc gaacacggtt tgatgtttac
    28561 cgcatactgc cgcacgctgc acaatatcga agcgcaatta ttgagtatgt tcggcgacac
    28621 ggatggcaaa accgacctgc tgctcctgca cctctccgcc gccgtttcgg gcggatacta
    28681 ctacgcgccg tctgtcgaac gcctgcaaaa cctctaagcc agacgatgcc gtctgaaacc
    28741 atttttcaga cggcattttc cc
//
LOCUS       DAWKQB010000010        25941 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.010, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000010 DAWKQB010000000
VERSION     DAWKQB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.010"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            52..1005
                     /locus_tag="WJL14_000329"
     CDS             52..1005
                     /locus_tag="WJL14_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ7655459.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1110..1433)
                     /locus_tag="WJL14_000330"
     CDS             complement(1110..1433)
                     /locus_tag="WJL14_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655460.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1606..2967)
                     /locus_tag="WJL14_000331"
     CDS             complement(1606..2967)
                     /locus_tag="WJL14_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ7655461.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3268..4968)
                     /gene="recJ"
                     /locus_tag="WJL14_000332"
     CDS             complement(3268..4968)
                     /gene="recJ"
                     /locus_tag="WJL14_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ7655462.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5037..5750)
                     /locus_tag="WJL14_000333"
     CDS             complement(5037..5750)
                     /locus_tag="WJL14_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655463.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTVMMWGMNSPFSETTARKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKQFQMVEPNPR
                     FAYQALPVKLESPASQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCV
                     SAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVG
                     AVLALPVAALIAATNSSDK"
     gene            complement(5797..6372)
                     /locus_tag="WJL14_000334"
     CDS             complement(5797..6372)
                     /locus_tag="WJL14_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ7655464.1"
                     /translation="MPSERGGILLKCAIGKNSRTKMFEQEDEWISKTQMKKQMNGLQD
                     LGMELTKLSNDTLKKIGLDEDLYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIE
                     AFLAKLRGDDAAHNAFLQRVEQARVRLLADDGALTQFMSDFPHADAGKLRTLIRNTKK
                     EQEQNKPPKNFRALFQELKTVMESQGGTGEA"
     gene            6463..7794
                     /gene="pmbA"
                     /locus_tag="WJL14_000335"
     CDS             6463..7794
                     /gene="pmbA"
                     /locus_tag="WJL14_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ7655465.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKATGATAAEADFSESLGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTYGFAAHRQSTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGNYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8092..8295)
                     /locus_tag="WJL14_000336"
     CDS             complement(8092..8295)
                     /locus_tag="WJL14_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ7655466.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8538..8840
                     /gene="clpS"
                     /locus_tag="WJL14_000337"
     CDS             8538..8840
                     /gene="clpS"
                     /locus_tag="WJL14_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ7655467.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8842..11121
                     /gene="clpA"
                     /locus_tag="WJL14_000338"
     CDS             8842..11121
                     /gene="clpA"
                     /locus_tag="WJL14_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003746132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ7655468.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNTEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DYALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLGLPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSMGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPGLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIAKVVDKFLLRLEHRLLDKKVEAE
                     FTPALRKYLARKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSNGGFVRIDWDAAK
                     EEAVLKFKKSKVKPETETV"
     gene            complement(11168..13039)
                     /locus_tag="WJL14_000339"
     CDS             complement(11168..13039)
                     /locus_tag="WJL14_000339"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ7655469.1"
                     /translation="MPPLAIRRSAKNLKKKFKKFCQFGFTGTPIFPENALGAETTAGV
                     FGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALLHPE
                     RIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLHPLK
                     VATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTDSKA
                     FQNYYRDLAKRVKNQEVDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNR
                     IYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDDRTGEVR
                     RGYLDVAKELRERFPDPDKIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALRELQSV
                     DAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLRRQKAGEQ
                     KEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRASIG
                     HRAKESLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRREAAELIAAEDLNETAAKR
                     YLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGTDI"
     gene            complement(13000..13371)
                     /locus_tag="WJL14_000340"
                     /pseudo
     CDS             complement(13000..13371)
                     /locus_tag="WJL14_000340"
                     /inference="COORDINATES: protein motif:HMM:NF037510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(13390..14265)
                     /locus_tag="WJL14_000341"
     CDS             complement(13390..14265)
                     /locus_tag="WJL14_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7655470.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENQLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQIINQFEQTGTYANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLGVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSA"
     gene            complement(14344..14925)
                     /locus_tag="WJL14_000342"
     CDS             complement(14344..14925)
                     /locus_tag="WJL14_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ7655471.1"
                     /translation="MGEVFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENSRILDNS
                     LKHISKSAVKGGKLFPAKSIMMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALD
                     INFAFYYFFIIAEQSKKLINTSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTL
                     THSISEGLPHEIALRRKQYEYYREQLLAFPKAT"
     gene            complement(14966..15562)
                     /locus_tag="WJL14_000343"
     CDS             complement(14966..15562)
                     /locus_tag="WJL14_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ7655472.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGDS"
     gene            complement(15728..16738)
                     /locus_tag="WJL14_000344"
     CDS             complement(15728..16738)
                     /locus_tag="WJL14_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ7655473.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(16735..18279)
                     /locus_tag="WJL14_000345"
     CDS             complement(16735..18279)
                     /locus_tag="WJL14_000345"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEZ7655474.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNADIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(18352..19356)
                     /gene="rfaD"
                     /locus_tag="WJL14_000346"
     CDS             complement(18352..19356)
                     /gene="rfaD"
                     /locus_tag="WJL14_000346"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ7655475.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKERL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEEFIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKAGVERYVKWMLENLA
                     "
     gene            complement(19397..20515)
                     /locus_tag="WJL14_000347"
     CDS             complement(19397..20515)
                     /locus_tag="WJL14_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7655476.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(20517..21752)
                     /gene="dcm"
                     /locus_tag="WJL14_000348"
     CDS             complement(20517..21752)
                     /gene="dcm"
                     /locus_tag="WJL14_000348"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7655477.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(21789..22760)
                     /gene="hldA"
                     /locus_tag="WJL14_000349"
     CDS             complement(21789..22760)
                     /gene="hldA"
                     /locus_tag="WJL14_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ7655478.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(22815..23555)
                     /gene="pyrF"
                     /locus_tag="WJL14_000350"
     CDS             complement(22815..23555)
                     /gene="pyrF"
                     /locus_tag="WJL14_000350"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ7655479.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(24081..24728)
                     /gene="adk"
                     /locus_tag="WJL14_000351"
     CDS             complement(24081..24728)
                     /gene="adk"
                     /locus_tag="WJL14_000351"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ7655480.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            24943..25782
                     /gene="htpX"
                     /locus_tag="WJL14_000352"
     CDS             24943..25782
                     /gene="htpX"
                     /locus_tag="WJL14_000352"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ7655481.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         6055 a         6767 c         6354 g         6765 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cacagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt cgaccgtgtc
      661 accaagctga ttgaaaaagg tctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtttctt tatccgtcca accgatacat caatcgaatt ttctgcatcg gattgcctca
     1141 tcttcgcaaa acattgccga agtttcgcgg ttgcacgcct gacgcacttt ctgccgcgcc
     1201 acgccttcat tccgatcgct tgcctcaaac gtcttctgaa aaggcgtaac gctgcataca
     1261 tacatggtgg aatttgaacc gaaaaggtcg attttgacgg tattgctgcc gctttccaat
     1321 acggcgacac ggtgttccaa ctgcgccaga cgtgcttcca aatcgccgat tctgtcggca
     1381 aatgcaggca tggcggcaca cgcggtcaaa atgcccaaaa tacatttttt catcaatttt
     1441 tccttgtttc ttgaactctg cccacccgga catccttttt ccgagaggca gggttagact
     1501 ttatttacgg ggatgaatcc ccttacaaat caggataata catagaacgg cactttatgc
     1561 ttcatcccat gtgttgaaac ttcattgctc cttgtctgtt gacccttatt cccaatccgt
     1621 tcccacaacc ttcggcttgg gtttgcgcct gcgacggcgg cgttttttcg cctgtccttc
     1681 gtttttttcg tgtcgggcgg cggcttcgtt tttggtcatc tccgtccgtt gttcctgcga
     1741 tgccgtctga aacgccgtcc accactcggc aagggcgcgg tccgcattgc cggtttcggc
     1801 gcgcaagagc aggaaatcat aggcggcacg gaaacgcgcc tgtgcaaaca gtttgtgcgg
     1861 ccttgcgcct ttgcggtttt caaactgcgg ctggaacatc caaatttcgc gcatcgtggc
     1921 ggaaaagcgt tgcggtacgc cccaaccgcg ttcgacggtt tcgcgcatcg tattgattgc
     1981 atcggacagg gcgggcacgg gtttcaaacc ctgttgcaga ttgcttttcc aatggcgttc
     2041 caactcgggc cacatcaggg ccgccagtac gaaaccgacc gaaaccgatt tgtcggcacg
     2101 cagccgctca tcggtatttt tcagggcaag caccgtcatt tttccggcga tgccgtctga
     2161 aacgcgcaag gcattgagca gcggatggat gtcgtccggt atgtcaaatc cgttcaaacg
     2221 ttttagacac tcgcgcgcgt gccctgaaaa cagcagtttc ataatttcgt cgaacagcct
     2281 cgctaccggt tcgtgcttca gacggcatat cgattcggca atcggtgcgg cggtttcttc
     2341 cgacagctca aagcccaatt tgcccgacag gcggatggcg cgcaaaatcc tgacagggtc
     2401 ttcctgatag cgttcggcgg catcgccaat cataaccagc ctgcgggcgg caacatcggc
     2461 aatcccgttg tggaaatcca aaatctcttc tttttccgga tcgtaataca aggcattgca
     2521 ggtaaaatcg cgccgcatcg catcttcttc gatgctgccg taggtgttgt ccttcataat
     2581 cctgccgtgc gcgttctgat gaactttcgc accgccgcga aacgtcgtta cttcgataat
     2641 ctccgcaccg ttcatcacat ggacgatttg aaaacgcctg ccgacgatgc ggctgcggcg
     2701 gaagagtttg tgcacctgtt cgggcatggc atcggttgcg acatcgaaat ccttgggttc
     2761 gatgccgagc agcaggtccc tgatcgcgcc gccgaccaca taagcctgaa accccgcccc
     2821 tttcagacgg cgtatgacgt tttcggcggc aaagctcaac atttcggcac ggatgttgtg
     2881 tctttcggca ggaatgaccg ttttactttc cgctttttta ctgctccgac cggaaggcag
     2941 catcttattc aaccattttt tcagcataaa tattctaacc cgaagagcgg catctccacg
     3001 ggcaaccgtg ttcagacggc atcaggcggt acgattccgc aagatgccgt aaccataaac
     3061 aaaaggcgta gaaactacgc cgtccgaatc acgccgtcct cgcggcggca acgcgtcatt
     3121 ataacagatt gccctccaaa aaaatcagac cctgcttttg tggcagagtc tgaaatttga
     3181 tgcgccttct ccgattggga acattcccgt cgtacaaaca ggcaaatgaa ggaacagaaa
     3241 tcacattcaa acagtgttcc gccgcctcta cgcggcttcc cagtaatcga tatacagctg
     3301 caattcgaga ttgttgcgcc attcgttggc aacggggcgg taaaccgtgc ggatgtattc
     3361 gggaatgtct tcgctgcaac gccaaaacat cgcttcaaat tcgcagccgt ctttttgcag
     3421 ccagactttt ttgtgtttgc cctccgcgcc caaaggttgc tggcggacga cgtggaactc
     3481 gtcggtaaag ctcggcggcg cgaagccctg cccccaaacg tgacgggcaa ggttttgcgc
     3541 ctgttccaac gtgatgtcgc aggcgggcag gctgccgtcg gtgatgaagg tttgcgacaa
     3601 atcgtcttcg cacaccattt cgcgcacggc ttcttcaaag gtcgtctgaa acgcgggaat
     3661 gttgtgttca agtatgctca aacccgccgc catcgcgtgt ccgccgaatt tcaaaatcaa
     3721 atcgggatgg cgtttggaca ccaagtccaa agcatcgcgc aggtgcaaat tgggaatgga
     3781 acgtcccgaa ccgcgtactt cgccgttgtc ggcaggcgca aacacgatgg tcggacgata
     3841 aaaacggtct ttgaggcggc tggcaacgat gccgaccacg ccttgatgga agtcgtcgcg
     3901 atacgccacc aaagtcatct gacctgaagg cagggtttca ggaaaagcat tcagcgcgtc
     3961 ttgcagcata gactgctcga tttcgcgacg ctcgatattg aggttgttta actgagccgc
     4021 cagttcctga gcttcggaat catctcgcgc caacaggcag gcgatgccga ccgacatatc
     4081 gtccagccgt ccggcggcgt tgatgcgcgg gcccaacgca aagcccatat caaacggctg
     4141 ggccttgcgc caatcgcgcc gcgccacttc aaacaaggcg cggatgccgg ggcgcatttt
     4201 gcccgaacgc atccgtttca aaccttgcga cacgaggata cggttgttgt gatcgaggga
     4261 gacgacgtcg gcaacggtac cgagtgcgac caaatccaaa aggtcgccca gattcggctc
     4321 ttttaggccg tctgaaaaat aattgcggcg gcgcaattcg gcacgcaacg ccatcaatac
     4381 ataaaaaatc acgcccacgc ccgccaagct tttgcttgga aaaccgcagc ctttttggtt
     4441 cggattgacg atgatgcagt cgggcacggt ctcggccggc aagtggtggt cagtaacgat
     4501 gacatccaaa cccagagcct gcgcgcgcgc cacgcctgcg atgctggcaa tgccgttatc
     4561 gaccgtaatc agcaaatcca cgccttgcgc ggcggcgatt tcggccagct cgggcgttaa
     4621 accgtagccg tgttcaaagc ggttgggcac gaggaaatct acttttgccc ccatcgccgc
     4681 caaaccgtcc aaaccgacgg cacacgcggt cgcgccgtcg gcatcgtagt cggcgacaat
     4741 caagattttt tcctgacgct caaccgcatc cgccaaacgg cgggcggcgg cttcgcaatt
     4801 cgtcagcgtt tgataaggca ggagggaagc gagtttgtcg tccaattcgg caggactttg
     4861 cacaccgcgc gaggcacaaa gccgggcgat taaaggatcg gcgccggcgg tgagcaaatg
     4921 attgaaaacg tcggtattga cggatcgggt ttggattttg acggacatag cgtggaactt
     4981 tacagtgcgg atgtgtttga aatgccgtct gaaggctttt cagacggcat tgccgttcat
     5041 ttgtctgagg aattcgtggc tgcaatcaac gccgcgacag gcaaggccag caccgcgccc
     5101 accgcatcca gtatcaacgt ggtgggcgta tatgcaatat tttcaaacaa cttggatttg
     5161 tcggtatgtt ttttcgtaac cgtgtaataa atatcggcag gcacactttg ctcaaaatga
     5221 taatcggcgt tcagtttttg cggtgtggcg tagtatttgc ctttggcgga gacgcagcgc
     5281 gtgtaaatgg tccgattgtc gagttcgacc gcttcaaact caagctgttt cagcttggcg
     5341 atgtcggcag gtctgtcggt atcgtagcgc aggcaaaggc cttcggtact gaaattctgg
     5401 ctggcgggcg attcgagttt gaccggcagg gcttggtagg caaagcgcgg gttgggctca
     5461 accatttgaa actgcttgtc caacccggcc ttcaaaatgc ccgtcagctt cgccgaatct
     5521 tcaggattga cgacgaacca gtatttcccg cccatcatca ccaggctgcc cttttccaat
     5581 tgggcattgt cttcggcaac cacaccgaag gcgcggattt ggtccttgtc aacgtgtttg
     5641 cgggcggtcg tttcgctgaa cgggctgttc ataccccaca tcattaccgt acagccgttc
     5701 aacatcaagg ttgccgccaa aacggcggcg gtcgttttac ggaacaacat tttatcctcc
     5761 aatctaaata acggcggatg tcgtctgaaa acatgcctag gcttcccctg ttccgccttg
     5821 gctttccata acggttttca attcttgaaa cagggcgcgg aaatttttcg gcggtttgtt
     5881 ttgctcctgc tcttttttgg tgttgcggat caaggtacgc aatttaccgg cgtccgcatg
     5941 cggaaaatcc gacataaact gcgtcaacgc gccgtcgtct gccaacagcc gtacgcgcgc
     6001 ctgttccacg cgttgcaaaa aggcgttgtg cgccgcatcg tcgccgcgca gcttggcaag
     6061 aaacgcctcg atgggcgcgg gatccgtgtc gcgcatcagc ctgccgataa attgcgcctg
     6121 ccgcttgagc gcgccgttgg acgtaatttt tttataggta acgacggctt cgtacaaatc
     6181 ttcgtccaag ccgatttttt tcagcgtatc gtttgagagc ttggtcagtt ccatacccaa
     6241 atcctgcaaa ccgttcatct gctttttcat ttgggtttta ctgatccact cgtcttcttg
     6301 ttcaaacatc ttggttctcg aattttttcc aatagcgcat tttaacagaa tgccgccgcg
     6361 ttcagacggc attttgcacc gcacgccctg caagcggcgc gcttttgccc ctgccgcccg
     6421 aaacggttaa aatgccgcaa agcatcatca agaaagattt ctatgctgtt caaccacacc
     6481 gcttccgaac tcctcgacct ctgccgccgc acgctcgact tggcaaaagc gacgggcgca
     6541 accgccgccg aagccgattt cagcgaatca ttgggacaaa gcgtcagcgt gcggctgggc
     6601 gaaatcgaac aaatcgagtt tcagcaggac aagtcgctgg acattaccgt ttacgtcggc
     6661 aaacgcaaag gccgcgccgg caccgccgat ttctccgaac aagccctgcg cgacaccgtc
     6721 cgcgccgccg ccgacatcgc ccgccacacc gccgaagacg gttgcgccgg acttgccgac
     6781 cccgagttga tggcgcaaca catcggcgac cccgaccttt accacgaatg ggatttggat
     6841 acggaagccg ccgtcggctt ggcaaaacaa tgcgaacaag ccgccctgaa cgaggatgag
     6901 cgcatcgaaa actccgaagg cgcggcggtg caaaccggcc attaccaata cgtttacggc
     6961 aacacctacg gttttgccgc acaccggcaa agcacgcacc acagcatttc ctgcagcgtc
     7021 gttgccgccg acgaaaacgg catgcagcgc gactactggt acgattccgc ctgccgccat
     7081 ccggatatgg acagccccga aaccatcggt caaaccgccg cccgccgcac tttgcgccga
     7141 ctcggcagcc gcagcatccc gacaggcaac taccccgtcc tcttcgatac caccgtttcg
     7201 ggcggtctta tcggacacct cgtcggcgca ctctccggcg gagcgctcta ccgccaaagc
     7261 agtttcctga tcgacagcat cggcaaaaaa gtcctgcccg atttcctcaa cctgcgcgaa
     7321 gaaccgcaca tcccccgctc tttccgcagc agctattttg atgcggaagg cgttgccacc
     7381 gcaccgcgct tcgtgattca aaacggcatt gtcgaaggct atttcctcag cagttacagc
     7441 gcgcgcaaac tcggaatgca gaccacgggc aacgccggcg gcgcgcacaa cctttatttg
     7501 aaccatacgc acgaaacgca atccgacctg ctgaaagaaa tggggacggg attgttggta
     7561 accgaactga tgggacaggg cgcgaacacc attaccggtg actactcgcg cggcgcggcg
     7621 ggtttttggg tggaaaacgg cgtgattgcc taccccgtcc acgagattac cgtagccgga
     7681 cgcttgcagg atatgtaccg cgacattgtc ggcgtggcgg atgacgcttt gcggcgttcg
     7741 tccaacaaaa tcggctcgat tctgattgcg gggatgacgg ttgccggaag ctgaacgccc
     7801 tgccgatgcc gtctgaagac gaaggcggca cgttttgccc cgtgcctgcc cgtttgccgt
     7861 tttatgccgc accgcctttt gccgacaaca ttgcacattt tttcatcagc actgattttc
     7921 ccttatccgt caaaatacta agccacttgt ccacaacagg gtacacacaa gttcaggata
     7981 aggcgtgcga ggattggggg tttttccgcc gccggcaaaa cggcagacaa aaacggcggg
     8041 acattgcctg tcccgccgtt ttcaaatata catttgaccg tacatcagga attaagcagc
     8101 ctgaatgttg gcggcctgtt tgcctttagg gccggtggtt acgtcgaaag agacgcgttg
     8161 gccttctttc agggttttga aaccttccat attgattgct gagaagtgag cgaacaaatc
     8221 ttcgccgcct tcatcaggag tgatgaaacc aaaaccttta gcgtcgttaa accattttac
     8281 gataccggtt gccattagaa acttcctata ctcaaaaatt aacaaaatca gcaaaacaag
     8341 gcatacggca aaccgagccg tctggcgttc cttccagctt gatatactcg gcggatgccc
     8401 aatcctgctt aaccgtcttt ttacatttgt taagacaaaa cgtcaagcta tgttttcaaa
     8461 aaagtgggaa aataggcaaa agtaccgaaa atacaacaaa aatgccgtct gaacgcggct
     8521 ttcaaatggg acacactatg accgcgcaac accaatccga cacgctcttg caccgtctga
     8581 acaccctgcc gccgaaacgt tacggcgttt tcctattgaa cgacgattac accacgatgg
     8641 aatttgtcgt cgaaatcctg accgaaatct ttatgctcgg acaagaacag gcggtagcgg
     8701 tgatgctctt ggttcatcac gaaggcaaag gcctgtgcgg cacttacacg cgcgatattg
     8761 cccaaaccaa acaacaacaa gtcatgcagc gggcaaaagc cgaagggcat ccgctgcaat
     8821 gtattgtcga ggagatttaa tatgcttgca cccgaattgg aacagatttt gcagcagctt
     8881 taccgcgagg cgcgtaaggc tcattatgaa tttatcagcc tcgagcatct gcttttggta
     8941 ctcatcgaag aagatgcctc cgtgccgaac gtcttaaaac tatgcggcgc ggatttgaaa
     9001 gtggtgtccg aacagctcgc cgccagcgtt gccgaaaaca cccccctgat tcccgaacac
     9061 cttttagaca cggtcgaaac ccggcccacg ctcggcttcc aacgcgtgat gcaacgggcg
     9121 atggtgcata cccagtctgc cggaaaagcc gcagtcgaac cgttggacgt tttggtcgcg
     9181 ctgatgagcg aaaccgacag ccacaccgtc tatttcctca agctgcaatc ggttacgcgt
     9241 tttgaagttt tgcgctgtat tgcccatggc tcgcccgatg acgaggatgg caatgattca
     9301 gacggcctgg gacgcgaggg cgaagaagcg gaacaaaaaa ccggctccct ttccgactac
     9361 accgtcaacc tcaacaccga agtcaaagcc ggccgtatcg accctttgat tggtcgcaaa
     9421 cacgaaatgg aacggctggt gcaaatccta tgccgccgcc gcaaaaacaa tccgcttttg
     9481 gtcggcgaag caggcgtggg caaaaccgcg ctggcggaag gtttggcaca tcaaatcgtc
     9541 aacggcggca ttccagacgc gcttaaagat gccgaagtgt acgcgctgga tatgggctcg
     9601 ctgttggcgg gcacgaaata ccgtggcgac tttgaagcgc gggtcaaatc cgtcttgaaa
     9661 cagctcgaaa aaatcccgca cgccattttg tttatcgacg aaatccacac catcatcggc
     9721 gcaggcagca ccagcggcgg caccatggac gcgtccaacc tgctcaaacc cgcgctggca
     9781 aaaggttcgc tgcgctgcat cggcgcaacc acctacgacg aataccgcac cattttcgac
     9841 aaagactatg ccttaagccg ccgcttccaa aaaatcgacg tggtcgaacc caccgtttcc
     9901 gaaaccgtac aaatcctgcg cggcttgaag ccgatgtttg aagccttcca ccaagtccgc
     9961 tatacgcaag gcgcactcga agccgccgcc gaactctccg cacgctacat caacgagcgt
    10021 ttcctgcccg acaaagccat cgacgtgatg gacgaagcag gcgcggcgca acggattctg
    10081 cccaaatcca aacagaaaaa agtcatcggc aaagcgcaaa tcgaaaccgt catcgccaaa
    10141 gtcgcgcgga ttccagaaaa aaccgtgtcg cacgacgaca aacaggtgct gcaattcctc
    10201 ggccgcgatt tgaaaaacat ggtttacggt caagaaaacg ccatcgacgc gctggttgcc
    10261 gccgtcaaaa tgtcgcgttc gggcttggga ctgcccgaca aaccgatagg cagcttcctc
    10321 ttctccggcc cgaccggcgt gggcaaaacc gaagccgcca aacagcttgc ctactcgatg
    10381 ggcgtaccgc tgcaacgctt tgatatgtcc gaatacatgg aacgccatgc cgtatcgcgc
    10441 ctcatcggcg cgcctccggg ctacgtcggc ttcgaacaag gcggcctttt gaccgaagcc
    10501 gtcaacaaac agccgcattg cgtgttgctc ttggacgaaa tcgaaaaagc ccaccccgac
    10561 attttcaacg tcctcctgca agtcatggac gcaggcaaac tgaccgacaa caacggcaag
    10621 agtgccgatt tccgcaacgt catcctgatt atgaccacca acgcgggcgc ggaaagcctc
    10681 agccgccccg gcctcggctt taccgccaag cgcgaacgcg gcgacgaaat gcaggccatc
    10741 aacaaactct tcacgcccga gttccgcaac cgcttggacg cgattatccc gtttgcaccc
    10801 ctgtccgaac ccattatcgc caaagtcgtg gacaaattcc tgctccggct cgaacaccgg
    10861 ctcctcgaca agaaagtcga agccgaattc acaccggcat tgcgcaaata tctggcgaga
    10921 aaaggtttcg acccgcaaat gggcgcgcgc ccgatgcacc gcctgattca ggaaaaaatc
    10981 cgcaaaccgc tcgccgacga actcctgttc ggcaaactat ccaacggcgg cttcgtgcgg
    11041 atagactggg atgcggcaaa agaagaagcc gtgttgaaat ttaagaaaag caaggttaaa
    11101 cctgaaacag aaactgttta accgattgaa aaataaaatg ccgtctgaaa tttcagacgg
    11161 cattttttta aatatctgtc cctattccgg caaacttttc cacaaacgcc gcaatctttt
    11221 gaaaaacact ttgtttcttc gtcagatatt gcgggttgag cgggctcatt ttcggcaggg
    11281 tttcggtcag ttccgtgccg ttttcgctgg catagccgcg tttgagcgag ccggtcaaat
    11341 aacgtttggc ggcggtttcg ttcaggtctt cggcggcaat cagctctgct gcttcgcgcc
    11401 tcatcacctc ttgagcgtag gtgtaaaagg tttccagtat ggcaggaacg tcggggactt
    11461 tgtccaaatc cgtatcgttg atgaaatcca caatcaggct ctctttggcg cggtggccga
    11521 tgctggcgcg gatgatgcgg cggatttctt ccaccagctc cgctttgcct ttgatttttt
    11581 tgtggtgctc gaaaaccagt tgcaagatgt aatccagatt gatttcctgc gatttgagca
    11641 aatccacctc aaaaaccaca tcgtcccaat cgatttttga ttgttctttc tgttcgcctg
    11701 ctttttggcg gcgcagccag tcgcggatgt cgttataggc agaacggtag tcctgcactg
    11761 ccctttcaga cggcatcggc acttgccgca tttcctgcac gtcttcatcg ctcaggtagt
    11821 atttttcttg aaacgccttc atcgcatctt cgtccgccgc gtccacactc tgcaactcgc
    11881 gcagcgcggc aaattcatcg tagttctgca atacgttttc cgcccgcagg tattcgccga
    11941 agagtttggc aaaatctttt ttgtcttttt ccgtttcgat tttgtcggga tcggggaaac
    12001 gctcgcgcaa ttcttttgcc acatccaaat aaccgcgccg cacttcgccc gtccgatcat
    12061 cgatatagcc gttcatgtat tcttcgtagc ttttttccaa caccacgttt ttggtgtttt
    12121 tatcgccgaa cagggtaatc gcatcaatgg ttgcctgctc caaatcgcgg aagcagacaa
    12181 tattgccgaa ggttttggtg gcatcgtaaa tgcggttggt gcgcgaaaac gcctgcatca
    12241 ggccgtgata gcgcaggttt ttatcgacaa acagcgtgtt cagcgtcggc gcgtcaaaac
    12301 ccgtcaaaaa cataccgacc acaatcagca aatccacttc ctggtttttc acccgttttg
    12361 ccaaatcgcg gtagtagttc tgaaaggctt tgctgtccgt gccgaaattg gttttgaaac
    12421 aggcgttgta atcgttgatg gcagcctgca aaaattcttt cgcgctgctg tccatcgctt
    12481 ccggttcaaa ggtctcatca acaatttcac cgacggcgtt ctgctcttcg ttggccgcaa
    12541 aggaaaaaat ggtggccact ttcagcgggt gcaggctgcc tgcctgttgt gttttgaacg
    12601 cttcgtaata gcacttcgcc gcatccacgc tgctgaccgc aaacatggcg ttgaagcctt
    12661 taccgcccgc attcaggcgg tgcgttttct gcctgaactg gttcaggata tattgcgtga
    12721 tttcgcggat gcgttcgggg tgcagcaggg cttggcggtt ttcggcagca ctcagtttct
    12781 tctcatcctg ttccgcttcc acggctttga actgcgggcg cacatcgttg taatccactt
    12841 tgaatttcaa tactttttca tcgcggatgg catcggtaat cacataagaa tgcaactcac
    12901 gcccgaacac gcccgccgtg gtttccgcgc ccaaagcgtt ttcgggaaaa atcggcgtac
    12961 cggtaaaacc gaactggcag aatttcttaa atttcttttt caggtttttt gcgcttcgcc
    13021 gaattgcgag cggtggcatt cgtcgaaaat aaatacaacc tgctgatggt aaaccggcag
    13081 attatcttca ctcttcatca ggttgttcag cttttggatg gtggtaacga tgattttgtt
    13141 gtcgtctttt tccaaattgc gtttcaagcc cgccgtgctt tccgaaccat tcacgctgtc
    13201 gggcgaaaaa cgttggtatt ccttcatcgt ctgatagtcc aaatccttcc tgtccaccac
    13261 gaagaaaacc ttgtcgataa acgccgattc cgtcgccaga cgcgccgcct taaagctggt
    13321 cagcgttttg ccgctgcccg tggtatgcca aacatagccg ccgctttccg gtttgctcca
    13381 attctttacc tacgccgagc tgttgatttt ccacaaaatg cgttcggcgg cggcaatctg
    13441 atacggccgc ataatcagca gcgtatcatt cgcatcgaac acgctgtaat gcagcaatac
    13501 gcccagcaat acgcttttct gcaggaacgt ggcggtaaag tctttcaaat ccttaatcgg
    13561 atgattgtcc gaccgcgccc aattcatcgt gaaatcaaag ctgtttttgt cgcgcttggt
    13621 ggtgttggcg aaatagcgcg tgtccgtgcc gttggaaatc acgaagattt gcaggaattt
    13681 gaacagcgaa ttttcgctgt tgaagctctc tttgctgtaa cggtgcacct gattgaatgc
    13741 ttcgcgcacc gccacgccgc gctttttcaa ttcaatctgc accagcggca ggccgtttac
    13801 cagcacggta acgtcatagc ggtttgcata cgtgcccgtc tgctcaaact gattgataat
    13861 ctgcacatgg ttgcgggcaa ggtttttctt gtccagcaga taaatgtttt tcagacggcc
    13921 gtcatcaaaa gcgaaatcat aaatatggtc gtcatggatt ttgcgggttt tatcggtaat
    13981 gttttcagac ggcctgtcca gatattccgt caaaaaccgc gcccattcgc cgtctgaaaa
    14041 cgccacatcg ttcagccgct gcaactgcgc gcgcaggttt tccagcagcc tgctttggct
    14101 gttcaaatcc ttgcggtatt cgtaaccctg attctgcaaa tcggcgatta actccgcttc
    14161 caactggttt tccgattggt agctgcccga ctgttcgatt ttttcatatt ggtcgagcac
    14221 gatgaaattc ggcgtttcgg cgatgggttt ggtttcgagg ttcatgggtc gtcctttgca
    14281 gtatcgggtt ttaaacggtt tgatgttcgg attgggctgc ctgaaaatgt gcaggctgct
    14341 ttttcaggta gccttaggga aggcaagcag ctgttcgcgg taatattcgt attgtttgcg
    14401 gcgcagggcg atttcgtgcg gcaggccttc gctgatagag tgggtcaggg tatcgaattt
    14461 gtctaggatg gcggcaattt tttcctgttc ggggagtggt gggatgggga tttttaattt
    14521 ctttaattct ttcatgctaa taataggtaa acttgaagta ttaataagtt ttttgctctg
    14581 ctccgcaata atgaaaaaat aatagaaagc gaaatttata tctaaagcat ccttaaattc
    14641 atctttaatt gtaaaattgg tcagttgttg gttactaata taattaactt ttattaacgc
    14701 atgctctcct atggttgccg ttgtcgacat cattattgat ttagcaggaa acagcttgcc
    14761 gcccttaact gcgctttttg atatatgctt caatgaatta tctaaaatac ggctattttc
    14821 cctaatatct tccattctaa accaagggat tgagccattt tcccaatatt ctttatttga
    14881 tttggaaggg gtatagccat tcttcaagtc aaacacctcc cccaacgtct tccaaaccac
    14941 atctttcaga cggcctttat agccatcagc tatccccccc gatttgattg tcaaaatcta
    15001 aaagaaagtc gcggtaatac cggtattggc gtttgcgcag ggctaattca gcttccagct
    15061 cggtgaattt gtcaagtatt tttacaattt tttgttgggt ttccaggggt gggataggga
    15121 ttaataattt tttcaagtta ctggcattta gagcaggaat acctgtaaat gtacataagg
    15181 catgaatctc ttgttcaaat tcaagtaata tatgatagag aaaacgaaca tccaattctg
    15241 ttctattctt aatagtaact gcataattta agttgccacg aaaatatctt ccttcttgcc
    15301 acgttattga gccgactccc gctcctcttg ttgtgatgcc gatagggtca ttttcagtgt
    15361 tccattcatc aatatagcca agcggctctt tgccactgtt aataactgga taactaccaa
    15421 tattatttga gattttctgc ttacttacag cttgtcctgt ttttatgata gctatcccat
    15481 tttctccacc caacggcttc cactccaccg gtgccgtctg aatcatctca atcaattttt
    15541 tcgctttgct ttgcatatcc atggttgttt tccgtttctt tcccgctgtg gcgggatgtg
    15601 gggcggttgt ggggtgcttc ggctcactct gtccgtttgc cgttatttct gcgggctgaa
    15661 gtccgtcctg ctttcaggct gcctgcaatg gtgtgcaggc tgctttttca ggtagcctga
    15721 atggtagtta ttttgtgcgg tcttttttct gtttgatctg ttgcacggct gttttttcca
    15781 acgctttaac gctttccaga taggcttgct ccaccggcga caggatgcgc gcttcatagg
    15841 cgcggtattc gcgttcggct ttctgctctg cctgtttgcg gctgatgctg cctgcaccct
    15901 gtaatgtgcc ttgtccgtac agcccggaaa atttgtccaa ttcggctatc cagtcgcgca
    15961 tatacatggg gctttgctcc tgcgctttga tttccgccag gtcgaagaag gcggaaacca
    16021 gacggttcag gcggaacagt tcgtcttcgg tcagatagtt tttggcgatt ttggcttcat
    16081 tcagcgtggg gattgcgcct tgaaaggtgg tcagccccat aaagtctttg ctgctgtcgg
    16141 cacggctgta tatcagctca gctgcggttt gccggctggc ggcatagtgc agtttgtttt
    16201 gaacggcggc aaaaaaggtt tggctttcgc tgcttttggg gttgtagtct tggctggtgg
    16261 catataaatc aagcacttgc cggtataggg ctttttcgct gctgcggatg tcgcgaatgc
    16321 ggttgagcag ttctttccaa tagtcgccgc cgcctgtgcc tttcaggcgt tcgtcgtcta
    16381 tggcaaagcc tttggtcaga tattcgtcca gccgttcggt tgcccattgg cggaattgga
    16441 tgccccgcgc ggaacggacg cggtagccga cggcaataat catgggcagg gaataatggg
    16501 cgattttgcg gtttaccttg cgcccgtttt cattttgaac tgtcaactgg aagttgacag
    16561 ttgccttctc ttccaattct tgctctgcaa gaatggtttt aatgtgtttg ctgatatttt
    16621 gtttggtggt ttggtacagt tctgccatgt ccgcctgcgt cagccaaagc tgtccgccaa
    16681 attcctgcaa ggcaaattgg gcagtgccgt cgttggcggt gtataggatg atgctcatgc
    16741 ttcaatctct gcaatcactt cgtcaatttc acgccgcagc cgttcgattt tggcaacggt
    16801 ttcgctgatt tcggcgttaa gctgtttgat gtcgataatt tcgcgggtgt cttcgggttc
    16861 aacatagctg ctgacggcga ggttgtagcc gttgtctttg acggtttgct gggcggcgtt
    16921 ttgggcgata tgcggcacat cggctttatc ggcgaagagt ttgacgattt cggcaatgtg
    16981 ttcttcggtt aagacgttgt tgttggtttc ttttttaaag aagccgcctg cgtcgatgaa
    17041 ttggatgtcg gcattgtctt tgtgtttgga caaaaccagt atattgacgg cgatgccggt
    17101 gccgtaaaag agattgggcg caagggcgat gacggtttcc acgtagttgc cctccaccag
    17161 atattggcgg attttctgct ctgcgccgcc gcgatagaaa atgccgggga atgagacgat
    17221 ggcggcgcgg cctctgccgg aaaggtagtt cagtgcgtgc agaatgaagg caaaatcggc
    17281 tttggatttc ggggcgagta cgcctgcagg ggcaaagcgg tcgtcgttga ttaaggtggg
    17341 gtcgccgctg cctatccagt tgatggaata gggcggattg gaaacgacgg catcaaaggg
    17401 tttgctgtct ttgagtttgg gattggtcag tgtgtcgccc aattcgatgt ggaatttgtt
    17461 gtaattgacg ttgtgcagaa acatattcat gcgggcgagg ttgtaggtgg tgtggttgat
    17521 ttcctgcccg aagaagcctt cttcgatgat gtgctcgtca aactgttttt tcgcctgcaa
    17581 gagcaggctg cccgagccgc aagctgggtc gtagattttg tttactttct cctgcccgtg
    17641 caccgccagc cgcgcaatca gcttggatac gctttgcggg gtgaaaaatt cgccgccgga
    17701 tttgcctgcg ttggcagcgt agttggaaat caggtattcg taggcatcgc cgaaaaggtc
    17761 gatgtggtgg tcttcaaaac tgccgaaatc gagttccgcc acgcctttta ggacggcggc
    17821 aaggcgtttg ttcttgtcgg caacggtgct gccgagccgg ctgctggtgg tgtcgaagtc
    17881 gtcaaacagg cctttaatgt cttgttcgga cggatagccg gaggcggagc tttcaatcgc
    17941 ggtaaaaatt tctttcagct tggtgttgag ctcttcgttt tgatgggctt cggcggcaat
    18001 attgcaaaaa agctggccgg ggtagatgaa atagcctttg actttgacgg catcgtcttt
    18061 gatttcgggc gtgatgatgc tgtccggcat agcggcgtaa tcaatactgc tgtcgcctgc
    18121 ctgcatatag tcggtaaagt tttcgctgat aaagcggtag aaaagtgtgc cgagaacgta
    18181 ttgtttaaag tcccagccgt ccaccgcgcc gcgtacttcg tcggcaattt tccaaatttg
    18241 gcggtgcagt tgggcgcgtt gttgcatttc ggtcatcatc gaaatccata taaaagttaa
    18301 acaaatcgaa atcgcctgat attttcagac gattttttac gggcattcaa attaagccaa
    18361 attttccagc atccatttga cgtagcgctc gacgcctgct ttgacatcga aaaattcttc
    18421 cgtataacct gcctcgcgca atttggtgat gtcggcttgg gtgaagcttt ggtatttgcc
    18481 tttgagcgcg tcgggaaatg ggatgtagcg gataaattct tcttctacca actcttttaa
    18541 actcatttca ggtttgcctt cggcggcgcg gcaggcgttg acggtggcag cggcgagttc
    18601 gttaaactgt tggctgcgac ctgttcctag gttgtagatg ccggaaagtt cgggatggtc
    18661 gaagaagtag aggttgactt tggcgacgtc ttcgacgctg acgaagtcgc gggtttgttc
    18721 gccgttgccg tagccatcgt tgctgccgaa caggttgacg taaccgtgtt cgcggtattg
    18781 gtggaaatgg tggaaggcga cggatgccat gcgacctttg tgttgttcgt gttgtccgta
    18841 aacattgaag tagcggaagc cgacgacttg ggcggtcagc ctttctttca tgcggcggcg
    18901 caatacttgg tcaaacagga atttggaata gccgtacaca ttaagcggtt tttccagttc
    18961 gcgctcttcg cggaagattt cgcctttgcc gtaaaccgcc gcactggagg cataaaggaa
    19021 gggaatgcgt tcgtcctgac accagtccaa caaatccagc gtgtactggt agttgttttc
    19081 catcatatac aaaccgtcgt gattcatggt gtcggaacac gcgccttgat ggaaaacggc
    19141 ttcgatgttt tgataaggta aaatgtgttc cctcacttgg cggatgaatt catgtttgtc
    19201 gagataatgg gcgatttcgc actcggcaag gtttttgaat ttttcgcctt tgctcaaatt
    19261 atcgacggca acaatgtcag taataccgcg ttgattaagt gctttgacga tgttgctgcc
    19321 gataaagccg gccgcgcctg ttacgatgat ggtcatattc ggtttccttt gttttgagta
    19381 aaattaataa acttagttac aagaaatcat cgatattgtc ctgaaacaca aatttgcgat
    19441 gatattccaa atatttttct cttccttcgg gatatattct catcttattt tttgcatttg
    19501 gattgatatt aagtttttcc catgtgtaac ggcttagtcg agtaccgcaa atcaaacgac
    19561 catcatccaa gaaagtaata taaccttgat cgaacaacca atcataagtc ggcgttaaag
    19621 ctaacccatt taaataatct aatgcctctt tctcattttt ctcagtaata caaaccatat
    19681 aaggtttaat atgactagcc cttaacaaaa tctcatctgt aattaatgta aatggacatt
    19741 gcggcatatg atttataaca tccttgcgat atttctgctg cccttttcta cttttctgtt
    19801 taatgatttc ttgctctacg aattgattaa attctgaagc cggtatttct aatttttctc
    19861 ttgataagag ttgcgggtgc acgatagagc gaaattgata atccaaaaat attcggaaat
    19921 aaaataatgg ttctgaatct tctatatctt tgacaggcaa tagtttaaga attgataaat
    19981 aactaatttt gggcaataca atcttacgcc aaattgacca tatatcatcc tccgaacgga
    20041 tataacccct acggtaacct ttagaatttt ccaaatggtc tgatacatca tggattgaaa
    20101 agaaaatatt ttctgtaggc agattattaa tacttaaaac gtaatcatca taaaaccttg
    20161 aaatatcatg atgatatttc tgttcttgtt tgtgatattc aacttttgca tcaaccaaat
    20221 aacgctgtaa atttttctta ttaaaaaaac aatttttctg aatgagagga tattctaccc
    20281 tccttccatt ttttgtctca gtaatccccc aattttcata ctcaaaaaat gtacttagtt
    20341 ttcgaccact atcatcaata taactaccta catatttgcg tgcttcccca ttaccaccaa
    20401 aaacagataa cttgttattc ctgtgaataa aactatcttc cgcacgtaaa ttttgaatac
    20461 tatcgacaat aaaatactct atgttttcaa tagtaattct ttgaaaatcc ggcattttac
    20521 tctccataaa gcgtaatatc catttgtttt aatatagcga ttaatacatc aacaacaata
    20581 ctgtttcccg cttggcgata catttgagta tcactaacta aaattttaaa atcatctcta
    20641 aaacccatta gccgcaaaca ttccctaggt gttaatttac gaatacgtcc acgattatga
    20701 gtaacataat tatcaacccc ggcacgatgc attttatgca tagtttgcaa taatgggcga
    20761 gctacaggca aatcagtttc cgtactggtt ttaaaatttt ttgttcctcc tgccaaaaca
    20821 taatttttga ttttttcaga aagataatat ttttcctcaa cgttatttac atcaaaaata
    20881 aagtcatcaa attcagattc gcgggctgcc tgaaaaataa aatcaccatg ccaattaaat
    20941 tgttgatttg ctttttggca taaggcaatt tctccattaa tctgtgtata acgtttttgt
    21001 ctatttttag aactggtaac aaatttcaca cccttttcac gtaaaaaata tttgctatca
    21061 gtatagtcct ccaaaaaatc ttgcatagta tgttctaatt caatcttttc aggaaactga
    21121 aaaccattta taggaggggt atgaaagcca acaacaaaaa tacgctcacg atgttgagga
    21181 atcccataat ccttactatt cattatttgg aaatataagt cataaccaag tgaataaaaa
    21241 acacttttta caactttcca agtttttcca ttatcatgat tgagcaagcc ctttacattt
    21301 tcataaataa atatctttgg ttggacttca tccacaacgc gagcaaattc atagaataat
    21361 gttcctcgtg tatcttctaa tcctgcacgt ttgccaacca tggaaaatgc ttggcaagga
    21421 ctgcctccaa ctaaaatatc aacttgattt ctaaactttc tcgcatcaaa ttgagtaatg
    21481 tcgttatgcc aaaaatcttc atttaattta tagtttccaa gataactttt tttaacgtat
    21541 ggatcaatat ctcctgcaaa aacaatggta tggtttaaat ttaatcggtg gaatgcctgt
    21601 tcaaccgcac caatcccgct gaatacggtt gctaatctaa tatgtgaatc aggtttaaga
    21661 aaagttttag atttccaacc ttgttgactg ggaaagagca aagttttttg taatcgagta
    21721 tcgtgtgtct gtgccattgt cgaaatagtc atacttatat cgttctgttt atcttatcaa
    21781 tatgaaaact acattgttga ttgccctgac aatgccttga tcaattcggc aaacgagcaa
    21841 accgccgtac cgagtttcgc cacgacaacc ccggccgcag tattggcaag gtacatggct
    21901 tcgggcatgg tgcagcctgc cgccaaaccc aagcccattc cggcaatgac ggtgtcgccc
    21961 gcaccggata cgtcgtaaac ttcttgggcg cgggtgggct ggtaaatcgg ttcgccttcg
    22021 ctgaacaagg tcatgccttc ttcgcttcgg gtcagtaaaa cggcggtcag gtcgaggtgg
    22081 cggcgcaggt tttgcgcttt ttcggtcagc tcgctttcgt ttttccaact gccgaccact
    22141 tctttcaatt cggcgcggtt aggcgtaatc agagttgcac cgacatattt ttcgtaatcg
    22201 tcgcctttgg ggtcgattaa gacggttttg ccggcgtgtt tcgcccaatc gatcatatcg
    22261 gagatatgcg acaggccgcc tttgccgtag tctgaaaaaa tgattgcgtc gtattcgggc
    22321 aagatttcgc ggtatttctg cttgatttgt tccaacactt cgcagttggg atgttcttca
    22381 aaatcaagac ggataagctg ctggttgcgg gcgacgacgc gcagtttgac ggtggtggcg
    22441 atttgtttgt cgcgcatcag ataggaggcg acgccgtcct gcaccatcag cgcatcgagc
    22501 gcgtcggcgg cttcgtcgtt gccggttacg gacaacagcc ctgccctgcc gcccaacgaa
    22561 gcgatgttgc gcgcgacatt tgccgctccg cccgcgcgtt ggtcgattcg tccgattttc
    22621 gccaccggca cgggggcttc gggcgaaata cgggacacat cgccgaacca atagcggtcg
    22681 agcatcacgt cgccgacaac caggactttg gcttgcgcga aacgggattt gagggtttct
    22741 tgttggaact tggcggacat attgcctccg ttgtattttt tcagacggcc tcagcctgta
    22801 aggccgtctg aaaatcagtt tgcttcaagg tttgccacgc ggttcacttc gcgcaatacg
    22861 gctaccggat cggcagcttg ggttacagga cgacccatta ccaaataagt cgaaccggca
    22921 gccaaggctt cggccggtgt catgatgcgg cgctggtcat cattattgcc ggcaacgtcc
    22981 aagcggatgc cgggcgtgac caagacaaaa tcctgtccca attcgcggcg cagcggcgcg
    23041 gcttcttggg cggaacagac cacgccgtcc aagcccgaac tttgcgccag ttttgccaag
    23101 cggatgactt gttcttcagg ggcggtgttc aaaccgattt ccgccaaatc gctttgttcc
    23161 atgctggtca acacggttac gccgattaag agcggcttcg tgccgtatcc ggcaacggct
    23221 tctgctgcgg cttccatcat acggcggccg cccgatgcgt gcatatcgac catccaaacg
    23281 cccatatcgg cagcgacttt gcaggcttgc gcgacggtgt ggggaatatc gtggtatttc
    23341 aaatcgagga aaagtttgaa accttgattg attaagcttt ctgccaaatt gcgtcccgtc
    23401 gcggtaaaca gctctttgcc gattttgatt tgacacaatg tcgggtcaag gttgcggacg
    23461 aatccgagcg tgtctttttc gttggaaaaa tcaagggcga cgataacggg ggtgcgttgt
    23521 tgcggagttt ggaagtcgga gattaaagga ttcatggtgt tttcgtttcg ggttttcaga
    23581 cgacctttat cgtctttttc gcagtatagt ggattaactt taaaccggta cggcgttgtc
    23641 tcgccttagt tcaaagagaa cgcttctcta aggtgctgaa gcacaaagtg aatcggttcc
    23701 gtactatctg tactgcctgc ggcttcgtcg ccttgtcctg atttttgtta atccactata
    23761 acaacgtaaa ttcaaaccgt aggtcgggca ttgatgtccg acctacgcga actcaagcct
    23821 ccgacaaact gtttcggata aatttgaacc ggtacatcag ccgtcattcc cgcgcaggcg
    23881 ggaatccatt gataaaactc agttcatcag atttaaacag cgattgccca aattcagaaa
    23941 tggattcccg cctgcgcggg aatgacgcca aatgattatt tttcggttgc aacacgctgt
    24001 ttatcaaaaa caggtcgtct gaaaacctga ttttctgttt tcagagtgcg aagcctgccc
    24061 gtgggtacga ccttttcgat ttatttgccc aatgcgccca atacttcggc tttcacggct
    24121 tctaccggct gagtgccgtc aactttgata tatttaggcg cgtgttcgcc ttccagtttg
    24181 ctgtaaaaat cgaccaaaac ttcggtttgc tcgtggtaaa cggcaaggcg ttttttcacg
    24241 gtttcttctt tgtcgtcgtc gcgctgaatc aaatcttcgc cggttacgtc gtctttgcct
    24301 tcaactttgg gcgggttgta ggtaacgtgg taagtacggc cggaagccaa atgcacgcgg
    24361 cggccgctca tacggtcgac aatcacgctg tcaggcacgt cgatttcaac gactgcatcc
    24421 aaatccacgc ctgcttcaac catcgcttcg gcttgtgcca atgtgcgcgg gaaaccgtcg
    24481 aacaggaaac cgtttttgca gtcgtcttgc gcgatgcgtt ctttgaccat gccgataatg
    24541 atgtcgtcgc gcaccaagcc gccttcgtca atgatttttt tcgcttccaa acccaagggc
    24601 gtgccagcct taatcgcggc gcggagcatg tcgccggtag agatttgcgg aatgccgaac
    24661 gcggcggtga tgaattgcgc ctgagtgcct ttgcccgcgc ccggcgcgcc taaaagtaat
    24721 gctttcatga agtgatcctt tgaaatgtgt ttattggaat caaacagatg gtttacgatt
    24781 ctacacggct tggcacaata gttcaaaaac caactgcaca aactgccatt tgcaagcaaa
    24841 tgttttgcat acctttgccg agtctgatat tcacaagccg gcaacttttt gggacaatcc
    24901 ccaaaatttc aaaacatcac accctaccaa aaaggaacat ccgtgaaacg aatttttctg
    24961 tttttggcta ccaatatcgc tgttttggtc gtaatcaaca ttgttttggc ggttttgggc
    25021 atcaacagcc ggggcggcac gggcagcctg ttggcgtatt ccgccgtcgt cggcttcact
    25081 ggttcgatta tttcgctgct gatgtccaaa ttcatcgcca aacaatcggt cggcgcggaa
    25141 gttatcgaca cgccgcgcac cgaagaagaa gcctggcttt tgaacactgt cgaagcccaa
    25201 gcgcggcaat ggaacctgaa aacgcccgaa gtcgccatct accactcccc cgaacccaat
    25261 gcctttgcca cgggcgcatc gagaaacagc tccctgatcg ccgtcagcac cggtttgctc
    25321 gaccatatga cgcgcgacga agtggaagcc gtattggcgc acgaaatggc acacgtcggc
    25381 aacggcgata tggttacgct gacgctgatt caaggcgtgg tcaatacctt cgtcgtgttc
    25441 ctgtcgcgca ttattgccaa cctgattgcc cgaaacaacg acggcagcca gtcccaggga
    25501 acttatttcc tggtcagcat ggtattccaa atcctattcg gcttccttgc cagcttaatt
    25561 gtcatgtggt tcagccgaca acgcgaatac cgcgccgacg cgggcgcggc aaaactggtc
    25621 ggcgcaccga aaatgatttc cgccctgcaa aggcttaaag gcaacccggt cgatttgccc
    25681 gaagaaatga acgcaatggg catcgccgga gatacgcgcg actccctgct cagcacccac
    25741 ccttcgctgg acaaccgaat cgcccgcctc aaatcgctct gagccgattt gaaacaacaa
    25801 aaaaaccgta cgcgcaagca gtacggtttt ttgtatatgc tgtctgaaaa catcaggttt
    25861 ccataccaac accaaccgta tccatttccg caccggatac gccgcattga tataccctga
    25921 taaaccgtat ccggacagaa a
//
LOCUS       DAWKQB010000011        25863 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.011, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000011 DAWKQB010000000
VERSION     DAWKQB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25863
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.011"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(41..313)
                     /gene="rpmA"
                     /locus_tag="WJL14_000353"
     CDS             complement(41..313)
                     /gene="rpmA"
                     /locus_tag="WJL14_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ7655482.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            complement(338..646)
                     /gene="rplU"
                     /locus_tag="WJL14_000354"
     CDS             complement(338..646)
                     /gene="rplU"
                     /locus_tag="WJL14_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ7655483.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            666..854
                     /locus_tag="WJL14_000355"
     CDS             666..854
                     /locus_tag="WJL14_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655484.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            870..1844
                     /gene="ispB"
                     /locus_tag="WJL14_000356"
     CDS             870..1844
                     /gene="ispB"
                     /locus_tag="WJL14_000356"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ7655485.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1849..2295
                     /locus_tag="WJL14_000357"
     CDS             1849..2295
                     /locus_tag="WJL14_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ7655486.1"
                     /translation="MNFSFAPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            2315..2386
                     /locus_tag="WJL14_000358"
     tRNA            2315..2386
                     /locus_tag="WJL14_000358"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2348..2350,aa:Arg,seq:cct)
     gene            2584..2685
                     /locus_tag="WJL14_000359"
                     /pseudo
     CDS             2584..2685
                     /locus_tag="WJL14_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            2737..4413
                     /gene="pilB"
                     /locus_tag="WJL14_000360"
     CDS             2737..4413
                     /gene="pilB"
                     /locus_tag="WJL14_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ7655487.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQSLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(4498..4707)
                     /locus_tag="WJL14_000361"
     CDS             complement(4498..4707)
                     /locus_tag="WJL14_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ7655488.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(4700..5332)
                     /locus_tag="WJL14_000362"
     CDS             complement(4700..5332)
                     /locus_tag="WJL14_000362"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ7655489.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEIFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            complement(5334..6194)
                     /locus_tag="WJL14_000363"
     CDS             complement(5334..6194)
                     /locus_tag="WJL14_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ7655490.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(6268..7500)
                     /gene="pilG"
                     /locus_tag="WJL14_000364"
     CDS             complement(6268..7500)
                     /gene="pilG"
                     /locus_tag="WJL14_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ7655491.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            7857..9500
                     /gene="pgi"
                     /locus_tag="WJL14_000365"
     CDS             7857..9500
                     /gene="pgi"
                     /locus_tag="WJL14_000365"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7655492.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNNSEGRAALHTALRLPDGA
                     GAVYADGRDVLPEIRRELNRALKFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDEVLCRLNPETTVFCVASKSFKTPETLLNAEAV
                     KAWYRGAGFSESETGCHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAACKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLERLTPYNLGMLMAAYEHKTFVQGVIWDINPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            9667..10488
                     /gene="dapD"
                     /locus_tag="WJL14_000366"
     CDS             9667..10488
                     /gene="dapD"
                     /locus_tag="WJL14_000366"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ7655493.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            10643..11428
                     /gene="fabI"
                     /locus_tag="WJL14_000367"
     CDS             10643..11428
                     /gene="fabI"
                     /locus_tag="WJL14_000367"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ7655494.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(11613..12611)
                     /gene="ilvE"
                     /locus_tag="WJL14_000368"
     CDS             complement(11613..12611)
                     /gene="ilvE"
                     /locus_tag="WJL14_000368"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ7655495.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLVPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            12921..13235
                     /locus_tag="WJL14_000369"
     CDS             12921..13235
                     /locus_tag="WJL14_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ7655496.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            13246..14415
                     /gene="lapB"
                     /locus_tag="WJL14_000370"
     CDS             13246..14415
                     /gene="lapB"
                     /locus_tag="WJL14_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ7655497.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINMHQTLLD
                     SPDTTGAKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            14379..14855
                     /gene="gloA"
                     /locus_tag="WJL14_000371"
     CDS             14379..14855
                     /gene="gloA"
                     /locus_tag="WJL14_000371"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ7655498.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(15028..17568)
                     /locus_tag="WJL14_000372"
     CDS             complement(15028..17568)
                     /locus_tag="WJL14_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ7655499.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAEIVADDLSALLQPAEAPAVEENVTETVAETSD
                     FHTAADDLSALLQPAEAPAVEENVTKTVAEIPDFNATADDLSALLQPSEVPAVEENAA
                     EITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAP
                     SEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            17876..18406
                     /locus_tag="WJL14_000373"
     CDS             17876..18406
                     /locus_tag="WJL14_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ7655500.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            18409..18699
                     /locus_tag="WJL14_000374"
     CDS             18409..18699
                     /locus_tag="WJL14_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ7655501.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            18699..18977
                     /locus_tag="WJL14_000375"
     CDS             18699..18977
                     /locus_tag="WJL14_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7655502.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSDGIIHALGIRAAAPDEYFHTAD"
     gene            19026..19892
                     /locus_tag="WJL14_000376"
     CDS             19026..19892
                     /locus_tag="WJL14_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7655503.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            19963..20721
                     /locus_tag="WJL14_000377"
     CDS             19963..20721
                     /locus_tag="WJL14_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7655504.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            20795..21286
                     /locus_tag="WJL14_000378"
     CDS             20795..21286
                     /locus_tag="WJL14_000378"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ7655505.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDIEK
                     DARYVANKIAHLRVFEDGEGKLNLSLKDVGGSVLLVSQFTLYADAASGRRPSFSKAAP
                     AEQAKRLYLHTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            21317..22327
                     /gene="dusA"
                     /locus_tag="WJL14_000379"
     CDS             21317..22327
                     /gene="dusA"
                     /locus_tag="WJL14_000379"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ7655506.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(22397..23284)
                     /gene="gluQRS"
                     /locus_tag="WJL14_000380"
     CDS             complement(22397..23284)
                     /gene="gluQRS"
                     /locus_tag="WJL14_000380"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ7655507.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPAPQGKQPAWRIRIPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(23301..23495)
                     /locus_tag="WJL14_000381"
     CDS             complement(23301..23495)
                     /locus_tag="WJL14_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655508.1"
                     /translation="MKLVSRNASNERIADRAFDFFSSGLMDGSATEEQYNALYDLTLL
                     EEPGMELNKDEIMALINSLK"
     gene            complement(23746..24801)
                     /gene="tal"
                     /locus_tag="WJL14_000382"
     CDS             complement(23746..24801)
                     /gene="tal"
                     /locus_tag="WJL14_000382"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ7655509.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGADEAQAQLAEIAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            24898..>25863
                     /locus_tag="WJL14_000383"
     CDS             24898..>25863
                     /locus_tag="WJL14_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ7655510.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         5764 a         6540 c         7386 g         6173 t
ORIGIN      
        1 gccgcatcgt gaaaagatgc ggcttcaggt atcggttgga ttattcttca gaaccggtgt
       61 aaggacggat gctgacagtt ttacggttca gcgcgccttt ggttttgaat tcgacataac
      121 cgtcaacttt ggcgaacaaa gtgtggtctt tgcccatacc tacgttgtcg cctgcgtgga
      181 atttggtacc gcgttggcgt acgatgatgg aacctgcggg aatcagctcg ttgccgtagg
      241 ctttaacgcc caagcgtttg gcttctgaat cgcgaccgtt gcgggtgctg ccgcctgctt
      301 ttttacttgc catttgtaat gctcctaagt tttaaggtta ggcgattgcc acgatttcga
      361 tttgggtgaa attttggcgg tggccttggc gtttttggta gtgtttgcgg cggcgcattt
      421 tgaagatgcg gactttttcg ccacgaccgt gtgccactac tttagccgtt acttttgcac
      481 cttcgataaa gggtgcgcca acttttacag attcgccgtc agcaatcatc aaaacttcgg
      541 tcagttcgat ttggctgtcg agttcggctg gtatctgttc tactttcaat ttttcgccga
      601 cggaaacttt atactgtttg ccgccggttt ttacgaccgc gtacatactc aactccataa
      661 gggttatggt taatatccgc acaccattgt gcggaactcg gcattgtatt gttatttgcc
      721 tgttttgtca aagtttgcgc ggttcggata accatatgcc gtctgaaaag atgtaccctg
      781 atggctttgc tgatataatt gcccgctatt tgaatcagct ttcaagcggt atctgccgtt
      841 tgacggaaac gtaaacctga gagtctgcca tgctcgagaa tctgccctat ttccagcgac
      901 atctgcctga agaccttgcc aaagtcaatg aagtcatcaa ccgtgcggtg caatccgatg
      961 tcgcactgat ttcgcaaatc ggtacatata tcatcagcgc gggcggcaaa cgcctgcgtc
     1021 cgattatgac gattttggcg ggtaaggcgg tcggttatga tgacgagaaa ctgtattcgc
     1081 tggcggcgat ggtcgagttt atccacactt ccaccctcct gcacgacgat gtcgtcgatg
     1141 aaagcgattt gcgccgtggg cgggcaacgg caaacaatct gttcggcaat gcggcggctg
     1201 tgttggttgg cgacttttta tacacgcgcg cctttcaact gatggttgcc tcgggcagta
     1261 tgcgcgtttt ggaagtgatg gcggatgcaa ccaacattat tgccgagggc gaagtcatgc
     1321 agctgatgaa catcggcaat acggacatta ccgaagaaca atatatccaa gtcatccaat
     1381 ataaaacggc aaaattgttt gaagctgccg ctcaagtcgg cgcaattttg ggcaaggctt
     1441 cccccgaaca cgaacgggcg ttgaaagact acggtatgta tgtcggtacg gcattccaaa
     1501 ttattgacga tgtgctggac tattctggcg aaaccgaaga aaccggcaaa aacgtcggcg
     1561 acgatttggc ggaaggaaaa ccgactttgc ctttgattta tctgatgcgt cagggttccg
     1621 aacaggttgc gaacgatgtg cgtactgctt tggaaaatgc agatcgcagc tattttgaga
     1681 aaatccacga ttatgtcgtc cgttcggatg cgttggcata ttcgataggc gaggcgcgca
     1741 aagcagtcga ttgtgccgtt accgccttgg atgccctgcc cgacagcgaa gtgaaggatg
     1801 ccatgattca gctggcgaag gaatctttgg tcagggtgtc ttgaggcgat gaatttcagt
     1861 tttgctcccc tgtttctggt tacgctgatt ctgttggggg tggtcagcaa caacaattcg
     1921 attaccatct cggcaaccat attgctgctg atgcagcaga cggcattgat acagtttgtc
     1981 ccgttggtcg agaagcacgg gttgaatctc ggtatcattc ttttgaccat aggggttttg
     2041 agtccgttgg tttcaggaaa ggcgcaggtt cctcccgttg ccgaattttt gaattttaaa
     2101 atgatatccg ccgtttttat cgggattttc gtggcttggc tggcgggacg cggcgtgcct
     2161 ttgatgggac agcagcctgt tttaattaca gggctgttaa tcgggacggt tatcggggtg
     2221 gcatttatgg gcggtatccc tgtcgggccg ctgattgcgg ccggcatctt gtcttttgtc
     2281 gtcggaaagg gttaaaatct ccttttcatt tcggctcgcc atagttcaac ggatagaacg
     2341 tatgcctcct aagcgtaaaa tacaggttcg attcctgttg gcgaggtttg acgatttgca
     2401 tttgtctgtt tcccgtgttg cgggaagttt ccgatataag gcctttcagt gttggagggc
     2461 ttttttgcca tctgaaaact ttttcttcct gcttgaaaaa ccgaccttta ggacggtaga
     2521 atcatgaaat gattttcagg cttcgtaaaa gatgttccgg cttggaaatc tgttgtttta
     2581 tgatatagtg gattaaattt aaatcaggac aaggcgacga agccgcagac agtacagata
     2641 gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata aaagctgtac
     2701 aggtataaca atgaataaat ttggggataa ggtcgtatga gcgtaggttt gctgaggatt
     2761 ctggttcaaa accaggtggt tactgttgag caggccgagc attactacaa tgagtcgcag
     2821 gcgggtaagg aagtgttgcc gatgctgttt tcagacggtg tcatttcgcc caagtcgctt
     2881 gcggcattga ttgcgagggt gttcagttat tcgattcttg atttgcgtca ttatccgcgc
     2941 cacagggtgc tgatgggggt gttgacggag gagcagatgg tggagttcca ctgtgtgccg
     3001 gttttccgtc ggggcgacaa agtatttttt gcggtttccg atccgacaca gatgccgcaa
     3061 attcagaaaa ccgtttctgc cgcagggatt gaggttgagt tggtcattgt cgaggatgac
     3121 cagttggcgg gtttgctcga ttgggtgggt tcgcgttcga catcgctgct tcaggagctt
     3181 ggggaggggc aggaggaaga ggaaagccac accctgtata tcgacaacga ggaggcagaa
     3241 gacggccctg ttccgaggtt tatccataag actttgtcgg atgccttgcg cagcggggca
     3301 tcggacatcc atttcgagtt ttacgaacac aatgcccgta tccgtttccg tgtggacggg
     3361 cagctccgcg aggtggttca gccgcccatt gcggtaaggg ggcagcttgc ttcccggatt
     3421 aaggtaatgt cgcgtttgga catttccgaa aaacggatac cgcaggacgg caggatgcag
     3481 ctgacctttc aaaagggcgg caagcctgtc gatttccgtg tcagcacatt gccgacgctg
     3541 tttggcgaaa aggtcgtgat gcggattttg aattccgatg ccgcgtcttt gaacatcgac
     3601 cagctcggtt ttgagccgtt tcagaaaaaa ttgttgttgg aagcgattca ccgtccctac
     3661 gggatggtgc tggtaaccgg tccgacgggt tcgggtaaga cggtgtcgct ctatacctgt
     3721 ttgaatattt tgaatacgga gtcggtaaat attgcaacgg cggaagaccc tgccgagatt
     3781 aacctgccgg gcatcaatca ggttaacgtc aatgataagc agagtctgac ttttgccgct
     3841 gctttgaagt ctttcctgcg tcaggacccg gacatcatta tggtcggtga gattcgtgat
     3901 ttggaaactg ccgatattgc gattaaggcg gcacaaacag ggcatatggt gttttccacc
     3961 ctgcacacca ataatgcgcc ggcgacgttg tcgcgtatgc tgaatatggg tgtcgcgccg
     4021 tttaatattg ccagttcggt cagcctgatt atggcgcagc gtcttttacg caggctgtgt
     4081 tcgagctgca aacaggaagt ggaacgcccg tctgcctctg ctttgaagga agtcggcttc
     4141 accgatgagg accttgcaaa agattggaaa ctttaccgcg ccgtcggttg cgaccgttgc
     4201 cgggggcagg gttataaggg gcgtgcgggc gtgtatgagg ttatgcccat cagcgaagaa
     4261 atgcagcgtg tgattatgaa caacggtacg gaagtggata ttttggacgt tgcctataag
     4321 gagggtatgg tggatttgcg ccgggccggt attttgaaag ttatgcaggg cattacttca
     4381 ttggaagagg taacggcaaa taccaacgat taggtttgag aatgaaaatg ccgtctgaag
     4441 cgtgtttgtt tcagacggca tttgactttc agggtgtttg ccgggaaggc ggggcggtca
     4501 gcggtatgcc atgtcgggtt cggatatttc cggcaaactt tccgtttggc cggaaaccgt
     4561 atatttcccg tctgcccatc cgcccaagtc gatcagtttg cagcgttgcg aacagaaggg
     4621 gcggaatgcg ttttcgggtt cccatactac tgctgtttga caggtcggac atttgacttg
     4681 aaggcgtgtt tgccgcgatt cagccattgt gttttccttg tgttggtttt gaggcgaaaa
     4741 tccctgaata aaacgcgtgc aggcgcattg ttttctcacg caggcttttg aggctgccgt
     4801 cattgagcag cacatcgtct gcaagcagca ggcgttcgga ttcggatgcc tgatggctga
     4861 tgacggcagc cacctcgccg cgcgtcagcc cgctgcgggc catcaccctg ccgatacgtt
     4921 tttccaaagg tgcgcttatg gtcaggacac gccgtatcag gctgataaat tgacgctttt
     4981 ccgtcagcag cggaatttcg acaatgccgt aagccgcatc ggtaaaggtt tcttgctgtt
     5041 ttttgatttc tgagaaaatc agcggcaaca tcacggattc gagcaaggct tttcgcgatg
     5101 gggaggcaaa gatttcttta cgcaatatgt cgcgtcgcaa caaaccctgt gtgtcgaaaa
     5161 cggtgtcgcc gaacagccgc ctgatttccg gcagggcgat gccgtctgaa gccgtcagcg
     5221 agtgcgccgc cgcatccgca tcgatacgcg gtacgcccaa atcggcaaaa cattgcgcgg
     5281 ctgccgattt gccgctgccg attccgccgg tcagtccgac ccataccgtc atcttacagc
     5341 accggatggg tcagccacca gttgaccgcc cgccatacgg aatcgtttgc cgtaaaaatt
     5401 atccagcccg aaactgtcag tgcggggccg aaggcaaaat gctgcccctt ggcgacgcgc
     5461 ataacgattg ccgcgaccaa accgatcagc gaggaaacaa aaatcagcac gggcaatgcg
     5521 gatatgccga gccacgcgcc caatgcggca atcagtttga aatctccgtt gcccataccg
     5581 atttcgcctt taatcagttt atataccgcg cataaaagcc atatcgaacc atagccggca
     5641 accgcaccta aaacggcaga ctgcaaaggc acgaagccgc cgtccaaatt aaatatcagc
     5701 cccagccaaa tcaagggtaa ggtcatcgag tcgggcaggt attgggtgtc cgcatcgata
     5761 aaggtcaggg aaatcagaaa cgcggtcagt atcaaaccgc ccaatgtaat ccaagaccag
     5821 ccgtattgcc aggcaaccag cccgaacaat acgccggtca gcagctcgat taagggataa
     5881 cgtatgctga ttttggtttg gcaggaagcg catttgccgc gcaggagcag gtagctgaca
     5941 atcgggatgt tctgccacgc gcgtatcggc acgcgacatt tgggacagca ggaatccggt
     6001 ttcatcaggt tgaaggtacg gctttcctct tcggtcagcg gcaggtttaa atattctttg
     6061 gcaaataccg tccagccgcg ttccatcatg accggcacgc ggtaaatgac gacatttaag
     6121 aaacttccga ccagcagccc gaacaccgct gccaaaggca cggcaaacgg cgacaataca
     6181 gacaaatcag acatattttg ttctcaatgt attcaaaaca aaaacaaacc ggcgcagagc
     6241 gaatccgcgc cggatctgtg cggcaaatca ggcgaccacg ttgcccaaat taaacagcgg
     6301 cagatacatg gcgaccagaa gcgtgccgat gaccaagcct aaaatcacga taatgatcgg
     6361 ctccatcata gcggacagcc tgccgaccgc attgtccacc tcgtcttcgt aaaattcggc
     6421 ggctttgttg agcatatcgt ccaaagaacc cgattcctcg ccgatggaag acatctgcaa
     6481 catcatattg gggaacagtt ccgtcgcacg catccccgaa gtcatagaca aaccttggat
     6541 gacgcgcgta cggatttccc gggtggcttc ttcatagatt aaattgcccg ccgcgccggc
     6601 agtggagtcc aatacatcga ccaaaggcac gcctgccgca atcagcgtcg ccgtcgtcct
     6661 gccccagcgg gcaatcgtcc ctttgcggac aatgtctccg aaaatcggca tacgcagcag
     6721 tatggcatcc atacgccgtt ggattttaat cgaacgcgcc ttcaatttaa ggaagccgta
     6781 tatggcaaag cccagtgcga tcagcaccat ccagccgtat gagacgaaaa agtcggacat
     6841 atccatcact gtttgggtca gtgcgggaag ctccgcgccc atattggcgt aaacttcttt
     6901 aaaggcgggc agtacgaaaa tcatcatcac gaataccaaa ccgatggcga cggcgatgac
     6961 ggataccgga taggtcagcg cggtttttac ctttttgcgg atggcctggg ttttttcttt
     7021 gtaaattgcc aatttgtcca gcaggctttc caatacgccg cccgtttcgc ccgccgcaac
     7081 caggttgcag tagaagcggt cgaaatattt cgggtggttt gagaatgcgc ggctcaacga
     7141 gctgccctgt tccacttcgc ctcggatttc catcagcatt tccgtcatag acgggttgcc
     7201 gtgtccgcgc gctacgattt caaatgcctg catcagcggc aggcccgctt taatcatcgt
     7261 ggacagctgg cgggtgaaaa cggtgatgtc ttcttgtgtg attttgcgct tggagcttgt
     7321 tttcacacgg gtaatctgca acgggcggat gccgcgtttt gccagttttt tgcgcgcctc
     7381 ttcttcggta aacgcggata cttcgccgtt gaccagtttg tcggaggcgg aatgcctgcc
     7441 ttcaaagata aagcgttttt ctttctttgc gaacaaagaa aatcctccgt ttttagccat
     7501 attctagccc cgtaaagtaa ttggaataaa atgtaagaaa catcgtgaaa aaacagtacc
     7561 ggcgtgttcc cggtaagatg aaaaccgccg acatcccgcc tgcgggcggc aaacgggaca
     7621 gaatcggatg cgattatacc ttatttaggc ggctgtccgg catttatgcg tacacaataa
     7681 atcttgcagg atattgttgc gggtcaaatg ccggccggag ggcatttccg ccatatggaa
     7741 ataaggtgct attggacgcg gcgggcggtg ttccggagat tcgccaaagc cgctgccgtt
     7801 tgttaaacta cattctgcta cattttaatc cggttctgaa aaatcaagga aaacagatga
     7861 atgcttttac ccgtgcatgg tatgcgctcg aacgccatta tcaggatacg cgtcatgtcc
     7921 ttttgcgcga ccgctttgcc tgcgaacccg accgttttga gcgtatgcat gagcgtttgg
     7981 acgggatgtt gttcgattac agcaaaaacc gtttgggcga agatacgctg caactgctct
     8041 gccgtcttgc ggagacggcg gatttggaag ggaaaatgcg tgctttgcgg acgggtgcga
     8101 aggtcaacaa cagtgagggg cgtgccgcgc tgcatacggc tttgcgcctg cccgacggtg
     8161 cgggtgccgt ttatgcggac ggcagggacg tgttgcccga aatccgccgc gagttaaatc
     8221 gtgcgttgaa gtttgcacac agtttggacg acggttcgta tcaggggaca accggaaaac
     8281 ggattacgga ttttgtccac atcggcatag gcggatccga cctcgggccg gcaatgtgcg
     8341 tgcaggcact tgagccgttc agacggcata tcgccgtcca ttttgccgcc aacgccgatc
     8401 ctgcctgcct ggatgaggtc ttatgccgtc tgaaccccga aacgacggtg ttttgcgttg
     8461 ccagcaagtc cttcaaaacg cctgaaaccc tgctcaatgc agaggcggtc aaggcttggt
     8521 atcgtggtgc ggggttctcg gaatcggaaa cgggatgtca tttttgcgcg gtatctgccg
     8581 atacggaagc ggcgcaaagt ttcggcattg cggcggaacg cgtgtttgcg atgtacgact
     8641 gggtgggcgg acgctattcc gtctggtcgc ccgtcggttt gcccgtgatg gttgcggtcg
     8701 gcggggcgcg tttccgcgag ttgttggcgg gggcgcacgc gatggacagc cattttttcc
     8761 acacgccgcc gcgccgcaac attcctgttc tgatggcact gattgccgtg tggtataaca
     8821 atttccaaca cgcggacggg cagaccgccg ttccgtacag ccacaacctg cgcctgctgc
     8881 cggcgtggct gaaccagctc gatatggaga gtttgggcaa aagccgcgct tcagacggca
     8941 gtcccgccgc gtgcaaaacg ggcggcatcg tgttcggcgg tgaaggggtc aactgccagc
     9001 acgcctattt ccaactgctc caccaaggca cacgcctgat tccctgcgac tttatcgtcc
     9061 cgatgacggc gcagggcgtg gaggacggac gcagccgttt taccgttgcc aacgcctttg
     9121 cccaagcgga agccttgatg aagggcaaaa ccttggacga agcacgcgcc gaactggcag
     9181 atttgcccga agcggaacgc gaacgcctcg cgccgcacaa agagttcccc ggcaaccgcc
     9241 ccagcaacag catcctgctc gaacgcctca cgccctacaa tctgggtatg ctgatggcgg
     9301 cttacgaaca caaaaccttt gtgcagggtg taatttggga catcaatccc ttcgatcagt
     9361 ggggggtgga atacggcaaa cagttggcga aaaccatcat cggcgaactg gaaggcggca
     9421 cgtccgtaca cgatgcctcg accgaagggc tgatggcgtt ttaccgtgaa tgccgtctga
     9481 aaggcggcgg tgcggcataa aggtactgcc gcccgcccga ttccgaaacg ccaatgttcg
     9541 gcaaccgccc gcgtattgct gacgaatatg cgtttgcgtg gcacaatagc gcattcattt
     9601 caaatgaaca tactgcccga aaataccggc aagcgtccca cgaaacatct cacataagga
     9661 aacattatgt ctttgcaaaa catcatcgaa accgcctttg aaaaccgcgc ggacattacc
     9721 ccgaccaccg ttactcccga agtcaaagaa gccgtgttgg aaaccatccg ccaactcgat
     9781 tccggcaaac tgcgcgttgc cgaacgcttg ggcgtgggtg agtggaaagt caacgaatgg
     9841 gcgaaaaaag ccgtgttgct gtccttccgc atccaagaca acgaagtcct caacgacggc
     9901 gtgaacaaat acttcgacaa agtgccgacc aagtttgccg actggtctga agacgagttc
     9961 aaaaacgcag gcttccgcgc cgttccgggt gcggttgccc gacgcggcag ctttgtggcg
    10021 aaaaatgtcg tgctgatgcc gtcttacgtc aacatcggcg catacgtcga cgaaggcgcg
    10081 atggttgata cttgggcgac tgtcggctct tgcgcgcaaa tcggtaaaaa cgtgcacttg
    10141 agtggcggcg tcggtatcgg cggcgtactc gaacccctgc aagccagccc gaccattatt
    10201 gaagacaatt gcttcatcgg tgcgcgttcc gaaatcgttg agggcgtgat tgtcgaagaa
    10261 ggcagcgtga tttctatggg cgtgttcatc ggtcaatcca ccaaaatctt cgaccgcacc
    10321 accggcgaaa tctaccaagg ccgcgtaccg gccggttcgg tcgtcgtgtc cggcagtatg
    10381 ccttccaaag acggcagcca cagcctttac tgcgccgtca tcgtcaaacg cgtggacgcg
    10441 caaacccgtg cgaaaaccag cgtgaacgaa ttgttgcgcg gcatctgatg ccttaaaccg
    10501 tatttgaaac gtccgatgcc gtctgaaatc cgcttcggac ggcattgccg tttgcatgct
    10561 gcaacgtgaa aacacagaaa cagggacaat ttgctataat caacggttta gaatgaaccg
    10621 aacactattt gaaggataca aaatgggttt tctgcaaggc aaaaaaattc tgattaccgg
    10681 catgatttcc gagcgttcca tcgcttatgg catcgccaaa gcctgccgcg aacaaggcgc
    10741 ggaactggcg tttacctacg ttgtggacaa actggaagag cgcgtccgca aaatggcggc
    10801 ggaattgggt tccgaactcg tattccgctg cgatgtcgcc agcgacgacg aaatcaacca
    10861 agtgttcgcc gacttgggca aacattggga cggcttggac ggtttggtgc attccatcgg
    10921 cttcgcgccg aaagaagcct tgagcggcga cttcctcgac agcatcagcc gcgaagcgtt
    10981 caacaccgca cacgaaattt ccgcatacag cctgcccgca ttggcaaaag ccgcccgtcc
    11041 gatgatgcgc ggcagaaact ccgccatcgt tgccctgagc tacttgggcg cggtgcgcgc
    11101 gattccaaat tacaacgtga tgggtatggc aaaagccagc cttgaggcgg gcatccgttt
    11161 taccgccgcc tgtctgggca aagaaggcat ccgctgcaac ggcatttccg ccggcccgat
    11221 taaaacgctt gccgcctccg gtatcgccga tttcggcaaa cttttgggac acgtcgccgc
    11281 ccacaatccg ctgcgccgca acgttaccat tgaagaagtc ggcaataccg ccgccttcct
    11341 gctgtccgac ctgtcgtccg gcattaccgg cgaaatcact tacgttgacg gcggttacag
    11401 cattaatgcc ttgagcaccg agggataatc cgccgttttc aaatccgtgc gccgtccgta
    11461 ccgcgtatcg gtttcgggcg gcggtttgcc gtctgaagcg tatttctagg gaaatgcccg
    11521 acttgctgca ggtgcagcgg aaatggtccg aacgcgaaaa tgccgtctga aacgccaaac
    11581 gggtttcaga cggcattttt ttatttaaag catcagcaca cttcaaccag ccagccgtat
    11641 ttgtcttccg ccaaaccata ctggatgtcg gtaatcgcct tacggatggc atagccgcgt
    11701 tcttggcttt tcacttcgat ttctttgccg ccgatgacga aggaagtaac gggcgagatg
    11761 acggctgccg taccggtcag aatcgcctcc gcaccgtttt ccaccgcagc tttgagttcg
    11821 tcaaccgtga aattgcgttc gctgacggta tagcccaaat ctttggcaac cgtcagtacg
    11881 gaatcgcggg ttacgccgtg caaaaattcg tcggtcagcg gtttggtaat gatttcatcg
    11941 ccgttaatca ggataaagtt ggacgcgccg gtttcctgca cgtcgccgtt cgggcagaag
    12001 aggacttggt ttgcgccgta ttcggctttc gccttcagca cccagtgcat ggcggaagcg
    12061 tagttgccgc cgcatttgac gcggcccata tgcggggcgc agcggatgtg ttcggtttcc
    12121 actaaaattt tgacgggcga gccgactttg aaatagtcgc cgacggggga agccaaaata
    12181 tacagcaggg cggtttcgga aggagaaccg gccttgccga tgacgggatc ggtaccgatt
    12241 aaggtcgggc gcaggtacag ggcggcaggc gcatcgggaa tttcgtcggc ggcacgtttg
    12301 accagttcga ccaaagcatc gagataggct tgggtttcgg ggcggggcag gtgcaaaatg
    12361 tccgcacttt gccgcatacg cgcgatattg gcagtcggac ggaacagtac gattttaccg
    12421 tctgcctgac ggaaggcttt cagtccctcg aaacattcgc tgccgtagtg cagggcgtgc
    12481 gcacccggta cgagggagag gtcttgggaa gattgccatt cggtcggctg ccatttgcct
    12541 tcgcggtagg cgaggacggg catttgactg tgaaaaacgc tgccgaatac ggcgggtacg
    12601 ggtctgctca tgatgtaaag cctttcttat tctgatatgt ttcaatgaac ggtttgaatt
    12661 tgaagattgt aaagatacgc ctgcaaacag ggttttgaca agtgcgcggc gggtttttct
    12721 gtcgatgcgg tgtccaatcc gttatttttc aaatggaaag gaacggtgta tttggtaaaa
    12781 ttgtcggcaa tcgcatactc cgtatgtcgt ccgaacacgc tgccgcatcc tatccgaaac
    12841 cgtgcaaatc gtttaaacta gcgcaatctt ggttcagagt gcgaagctgt ccgggcggcg
    12901 tttttattta cggagcaaac atgaaactta tctataccgt catcaaaatc attatcctgc
    12961 tgctcttcct gctgcttgct gtcattaata cggatgccgt taccttttcc tacctgccgg
    13021 ggcaaaaatt cgatttgccg ctgattgtcg tattgttcgg cgcgtttgtc gtcggcatcg
    13081 tgttcggaat gtttgccttg ttcggacggt tgttgtcgtt acgtggcgag aacggcaggt
    13141 tgcgtgccga agtaaagaaa aatgcgcgtt tgacggggaa ggagctgacc gcaccaccgg
    13201 cgcaaaatgc gcccgaatct gccaaacagc cttgagaaag ccgatatgga caatgaattg
    13261 tggattatcc tgctgccgat tatccttttg cccgttttct tcgcgatggg ctggtttgcc
    13321 gcccgcgtgg atatgaagac tgtattaaag caggcaaaaa gcataccgtc gggattttat
    13381 aaaagtctgg atgccttggt tgaccgcaac agcgggcgcg cggcaaggga gttggcggaa
    13441 gtcgtcgacg gccggccgca atcgtatgat ttgaacctca ccctcggcaa actttaccgc
    13501 cagcgtggcg aaaacgacaa agccatcaat atgcaccaaa cattgcttga ctctcccgat
    13561 acaaccggag ccaagcgcgc gcgcgtcctg tttgaattgg cgcaaaacta ccaaagtgcg
    13621 gggttggtcg atcgtgccga acagattttt ttggggctgc aagacggtga aatggcgcgt
    13681 gaagccagac agcacctgct caatatctac caacaggaca gggattggga aaaagcggtt
    13741 gaaaccgccc ggctgctcag ccatgacgat cagacctatc agtttgaaat cgcccagttt
    13801 tattgcgaac ttgcccaagc cgcgctgttc aagtccaatt tcgatgccgc gcgtttcaat
    13861 gtcggcaagg cactcgaagc caacaaaaaa tgcacccgcg ccaacatgat tttgggcgac
    13921 atcgaacacc gacaaggcaa tttccctgcc gccgtcgaag cctatgccgc catcgagcag
    13981 caaaaccatg catacttgag tatggtcggc gagaagcttt acgaagccta tgccgcgcag
    14041 ggaaaacctg aagaaggctt gaaccgtctg acaggatata tgcagacgtt tcccgaactt
    14101 gacctgatca atgtcgtgta cgagaaatcc ctgctgctta agtgcgagaa agaagccgcg
    14161 caaaccgccg tcgagcttgt ccgccgcaag cccgacctca acggcgtgta ccgcctgctt
    14221 ggtttgaaac tcagcgattt ggatccggct tggaaagccg atgccgatat gatgcgttcg
    14281 gttatcggac ggcagctaca gcgcagcgtg atgtaccggt gccgaaactg ccacttcaaa
    14341 tcacaagtct ttttctggca ttgtcctgcc tgcaacaaat ggcagacgtt tacgccaaac
    14401 aaaatcgaag tttaaccact accgagagga acacaaaaat gcgcttactc catactatgc
    14461 tccgcgtggg caatctcgaa aaatccctcg atttctacca aaacgttttg ggtatgaaac
    14521 tgctccgccg aaaagattat cccgaaggca gatttaccct tgccttcgtc ggttacggcg
    14581 atgaaaccga cagcacggtt ttggaactga cgcacaactg ggatacggaa cgatacgact
    14641 tgggcaacgc ctacggacac atcgcggttg aagtggacga tgcctacgaa gcctgcgaac
    14701 gtgtgaagcg gcagggcgga aacgtcgtcc gcgaagccgg cccgatgaaa cacggcacaa
    14761 ccgtgatagc cttcgtcgaa gaccccgacg gatacaaaat cgagttcatt caaaagaaaa
    14821 gcggtgacga ttcggttgcc tatcaaactg cctgataccg ccgccgccaa tgccgtctga
    14881 agcctttagg ggtttcagac ggcattttgt tgccgtcgac ctgctgtttg agcctgtgcc
    14941 ggttcaaact ttatccgtta caccgataag gcaaaaaaag atgccgtctg aaacggcatc
    15001 cttgatctgc gaaagggcag ttgggaatca aatacccaat tcctgcgcca atgcttgggc
    15061 acgtttgagt acgtcgcctt ccgcttcttc cagcaatttc tgcactgtct cggcagcggc
    15121 atcgcggtcg ccgatttcga gatacatttc ggcaaggtcg tatttcgctt cggaaggcgc
    15181 gtcagaacct acagattccg aagggaaact ggtatctgca ttatttggga tattttcttc
    15241 cgagaggtag atgctccaat ctaccgtttc ctcctcgccg tctttcagga agtcgggcaa
    15301 agcgtctgcc tcagaggtgt tggaatcagg cgtttccaaa gtgatttccg ctgcattttc
    15361 ctcaacggcc ggtacttcag aaggttgaag taatgcggac aaatcgtctg cggtggcgtt
    15421 gaaatcaggt atttcggcaa cggttttcgt tacattttcc tcaacggccg gtgcttcagc
    15481 aggttgcaac agtgcggaca aatcgtctgc ggcggtgtgg aagtcggacg tttcggcaac
    15541 agtttccgtt acattttcct caacagccgg tgcttcagca ggttgcaaca gtgcggacaa
    15601 atcatcggca acgatttccg ctgcattttc ctcaacggca ggtacttcag aaggttgaag
    15661 taatgcggac aaatcgtctg cggtggcgtt gaagtcgggt gtttcggcaa cggtttccgt
    15721 tatattttcc tcaacggacg gtgcttcggc aggttgaagc aatgcggaca aatcgtctgc
    15781 ggcggcgttg aaatcgggcg tttcaggcgc agtttccgcg acggcatcgg tttcgtacac
    15841 tttcaggaaa tcgtgcaact cttccggtgt ttggacttcg gcaactgttt tttccaagat
    15901 ggtttcgggc gaggaagcct tcaggaagcc tgccagtccg gagggtgagg caggttttgc
    15961 ggaagctgtt tcttctgtgc cgatatggtt gtttgagggc aggttgtcgg agaaatcggt
    16021 atcgacggtt tccggtttgt tttcggcagt ttgggcgaca gattccggtt cgggcgtgtc
    16081 gatgacgatt tcgacagggt tgtacgggtt gaaggtctcg ggctcgtaca cgctgtctgt
    16141 ggattcgatg gcgttccaat cggcatccgc gcgtttttgg gtttcttcat cctgcgtaag
    16201 tgcgccggat aaaatgccgt tttgcgcggc tgccaggctg tcgaaatcca agtcgatgcg
    16261 gttggaaggc gtatcggttt cgacatcgaa cgtttgtttt gccgacaact cttcttcaga
    16321 ttccccatct aaggcaagtg tgtcgtttac atcgtttttc ggagcgggtt cgggcgttgc
    16381 cggagtttcg acttcggcaa aggtgatttc tatgccgtcg tctgccgcgt cgtcaaggtc
    16441 aggctcttcc tcagggacgg attcttcggt acggcgcgcg cgtttggatt gggcaaggcg
    16501 caaaagcagc agcagggcga tcaatgccgc gcctccgccg gcaagcagca aggtgtacga
    16561 accgccgaac agtccgcttt cggtttcttc ttcggcagaa acctgttcga caggttcgga
    16621 aacggcgtta ccggtttcgt cggtcggcgt gtcgatggca gaagcggcgg cttcttgggg
    16681 ggcggattcg gcagcggttt ccgatgcggc agtatttgca gcgggtacag gttcgggtcg
    16741 aacggccggt ttttccgctt ttgcttcggg cgcggcaact tttgcttcag gtttttcaac
    16801 cggtttctct accgttgcct gtttggacgg ttcggacggc atggatgcgg tttcggcttt
    16861 gggtttcgcc gtttgcggtt tgggttgttc cgctttgatc ctgttcagat tcggaatgtg
    16921 aagcacgctg cccgcacgca gtctgccgtg tgcggaaaca tttgggtttg ccttcagcag
    16981 cgcatcggca acctgttcga gcgtcaggtg tttcgggcgg atggcggcgg caatctgttt
    17041 gaccgtttcg cctttgcgga cggtatgggt tttgccgttg tacgccggtt tgacggctgc
    17101 gttcgcgctg tcttttttat cggttttgcg gagggctttg gcgttttgat tttcttggga
    17161 ctctgctgtc ggagcggttt tgcggtgtgt cttgccgtct gaaagtgcag atttggtttt
    17221 gggcgagtag ccgacaggat cgaggatggc ggtgtattcg cgtacctgtg cgcctgcgcc
    17281 gatgcggaat accaggacgg gatcgcggac tgcctgttcg gaagaaacgg caatgacggc
    17341 tttgtcgccc aacttgtgga ctttggcggt caggcctttt tcggaaacgg taacgctgcc
    17401 gccgcctagc agggctttgg cttcttcgcc ggttacggta atgctgccgg aaaagggttc
    17461 gtcaaggttg gactggatat tcagtccgcc cagtccagca tgtgcctgaa aggatgcggc
    17521 aactgcgacg gaggcggcaa tcagtttgat ttgtctgttg tttttcaaga tgtatcccct
    17581 gtgggttggc ggctgaatac ggtttgaccg cgtacagtct gtaaatttcg tcatcatcgg
    17641 gcatcggcgg ggcagtcggc cggcgggcat ttaatatgtg aatgtaccga ccgccgccac
    17701 attttaaacg gcaatcattc gccgttttta caaattatga catatctcca tcttttttca
    17761 aaaacatctg tgcatatttg catcaatcaa aacaaaattt gttggttttg caggtgcaaa
    17821 aacagggttc tgcctgtatg attagcgttt atttgatttg ctttctcatt tggatatgaa
    17881 attcgtcagc gaccttttgt ctgtcatctt gttttttgct acttataccg ttaccaaaaa
    17941 tatgattgcc gctacggcgg ttgccttggt tgccggtgtg gttcaggcgg cttttctgta
    18001 ttggaaatat aaaaagctgg atacgatgca gtgggtcgga ctggtgctga ttgtcgtatt
    18061 cggcggcgca accattgttt tgggcgacag ccgcttcatt atgtggaagc cgagcgtttt
    18121 gttttggctg ggcgcgctgt tcctgtgggg cagccacctc gccggtaaaa acggcttgaa
    18181 ggcgagtatc ggcagggaga ttcagcttcc ggatgccgta tgggcgaaat tgacgtatat
    18241 gtgggtcggt ttcctgattt ttatgggtat cgccaactgg tttgtgttta cccggttcga
    18301 gtcgcaatgg gtcaactata aaatgttcgg ctcgactgca ctgatgcttg ttttctttat
    18361 tattcagggt atttatctga gtacctgtct gaaaaaggag gattgactgt ggaatatttt
    18421 atgttgctgg caacagacgg ggagaatgtg tatgaagcgc gtatggcggc acgtcccgaa
    18481 cacctcaaac ggctggagac gctgaagtcg gaaggccggc tgttgacggc aggcccgaat
    18541 cctttgccgg aggactccaa ccgcgtttcg ggcagtttga ttgtggcgca gttcgagtct
    18601 ttggatgcgg cagaggcgtg ggcggaagac gatccctatg ttcatgcagg cgtgtacagc
    18661 gaagtgctga tcaagccgtt taaagcggtg ttcaaataat gccggccgtc gatttgatcc
    18721 gcgaacgcct gcagacgctc gatccgctgg tgttggaaat cggcgatgag agccatctgc
    18781 acaaaggaca cgcgggcaat accggcggcg gacattatgc cgttttggtc gtcagcggcc
    18841 gttttgaagg cgtaagccgc ctgaaccgcc agaaaacggt caaatcgctg ctcaaagatt
    18901 tgttttcaga cggcatcatc cacgcgctcg gcatccgggc ggctgcccct gacgagtatt
    18961 tccatacggc ggactgaatg aagtctgccc gaacatttca atttaaaatt taaagagaga
    19021 agattatgaa agcaaaaatc ctgacttccg ttgcactgct tgcctgttcc ggcagcctgt
    19081 ttgcccaaac gctggcaacc gtcaacggtc agaaaatcga cagttccgtc attgatgcgc
    19141 aggttgccgc attccgtgcg gaaaacagcc gtgccgaaga cacgccgcaa ctgcgccaat
    19201 ccctgctgga aaacgaagtg gtcaacaccg tggtcgcaca ggaagtgaaa cgcctgaaac
    19261 tcgaccggtc ggcagagttt aaaaatgcgc ttgccaaatt gcgtgccgaa gcgaaaaagt
    19321 cgggcgacga caagaaaccg tccttcaaaa ccgtttggca ggcggtaaaa tatggcttga
    19381 acggcgaggc atacgcgctg catatcgcca aaacccaacc ggtttccgag caggaagtaa
    19441 aagccgcata tgacaatatc agcggttttt acaaaggtac gcaggaagtc cagttgggcg
    19501 aaatcctgac cgacaaggaa gaaaatgcaa aaaaagcggt tgccgacttg aaggcgaaaa
    19561 aaggtttcga tgccgtcttg aaacaatatt ccctcaacga ccgtaccaaa cagaccggtg
    19621 cgccggtcgg atatgtgccg ctgaaagatt tggaacaggg tgttccgccg ctttatcagg
    19681 caattaagga cttgaaaaaa ggcgaattta cggcaacgcc gctgaaaaac ggcgatttct
    19741 acggcgttta ttatgtcaac gacagccgcg aggtaaaagt gccttctttt gatgaaatga
    19801 aaggacagat tgcgggcaac cttcaggcgg aacggattga ccgtgccgtc ggtgcactgt
    19861 tgggcaaggc aaacatcaaa cctgcaaaat aattctgaaa acgggatacg gcggcaagac
    19921 gttcagacag gcgttttgcc gccgcgcagg acagggaata ccatgaaaca gaaaaaaacc
    19981 gctgccgcag ttattgctgc aatgttggca ggttttgcgg cagccaaagc acccgaaatc
    20041 gacccggctt tggtggatac gctggtggcg cagatcatgc agcaggcaga ccggcatgcg
    20101 gagcagtccc aaaaaccgga cgggcaggca atccgaaacg atgccgtccg tcggctgcaa
    20161 actttggaag ttttgaaaaa cagggcattg aaggaaggtt tggataagga taaggatgtc
    20221 caaaaccgct ttaaaatcgc cgaagcgtct ttttatgccg aggagtacgt ccgttttctg
    20281 gaacgttcgg aaacggtttc cgaaagcgca ctgcgtcagt tttatgagcg gcaaatccgc
    20341 atgatcaaat tgcagcaggt cagcttcgca accgaagagg aggcgcgtca ggcgcagcag
    20401 ctcctgctca aagggctgtc ttttgaaggg ctgatgaagc gttatccgaa cgacgagcag
    20461 gcttttgacg gtttcattat ggcgcagcag cttcccgagc cgctggcttc gcagtttgca
    20521 gcgatgaatc ggggcgacgt tacccgcgat ccggtcaaat tgggcgaacg ctattatctg
    20581 ttcaaactca gcgaggtcgg gaaaaacccc gacgcgcagc ctttcgagtt ggtcagaaac
    20641 cagttggaac aaggtttgag acaggaaaaa gcccgcttga aaatcgatgc cattttggaa
    20701 gaaaacggtg tcaaaccgta atggcatttc cgataccgat gccgtctgaa gccttttcag
    20761 acggcattgc acgttcaggt aaggaggacg gcttatgcgt gcggtcatac agaaaacggt
    20821 aggtgcaaag gtggatgtcg tgtccgaaaa cggcacggaa acctgcggca aaatcgacgg
    20881 cggattcgtc gtactgctcg gtgtaacgca tagcgacata gaaaaagatg cacgctatgt
    20941 cgccaacaaa atcgcccatt tgcgcgtgtt tgaagacggt gagggcaagt tgaacctgtc
    21001 tttgaaggat gtcgggggat ccgtgctgct ggtgtcgcag tttacgcttt atgccgatgc
    21061 ggcaagcggc cggcggccgt cattttcgaa agccgcacct gcagaacagg caaaacggct
    21121 ttatctgcat acggcagaac tgttgcgcgg gcacgggatt catgtcgaaa cagggcgttt
    21181 ccgcacgcat atgcaggtgt cgctctgcaa cgacgggccg gtaactatac tgctggactc
    21241 tttcatgacg cggatttccc caaaaatgaa ggttgttccg gattgaaatt gaatccgcaa
    21301 tgatgaaata tcgacaatga acgacaatac acacaccctt cccccgcgcc gcctttccgt
    21361 cgccccgatg ctcgactgga cggacaggca ctaccgttat atggcgcgcc agattacccg
    21421 caatacttgg ctgtacagcg aaatggtcaa cgccggcgcg attgtttacg gcgacaaaga
    21481 ccgctttttg atgtttaacg aaggcgagca gcccgtcgcc ctgcaactgg gcggcagcga
    21541 cccgtccgat ttggcgaaag ccgccaaagc cgccgaagaa tacggctaca acgaggtcaa
    21601 cctcaactgc ggctgcccca gtccgcgcgt ccagaaaggc gcgttcggcg cgtgtctgat
    21661 gaacgaagtc gggctggttg ccgactgcct caacgccatg caggacgcgg tcggtattcc
    21721 cgttaccgtc aaacaccgca tcggtgtgga caggcagacc gaataccaaa ccgttgccga
    21781 tttcgtcggc acgctgcgcg acaaaaccgc ctgcaaaacc ttcatcgtcc acgcccgcaa
    21841 cgcttggctg gacggtcttt cccccaaaga aaaccgcgac gttcccccgt tgaaatacga
    21901 ttacgtttac cgcctcaagc aggagtttcc cgggctggaa atcatcatca acggcggcat
    21961 caccaccaac gaagcaatcg ccggacacct gcaacacgtt gacggcgtga tggtcgggcg
    22021 cgaggcgtac cacaacccga tggtgatgcg cgaatgggac agattgtttt acggcgacac
    22081 tgcgaacccg atcggatacg ccgatttggt gcaacgcctc tacacataca gccaagccca
    22141 aatccaagcc ggacgcggca caatcctgcg ccatatcgtc cgtcacagcc tcgggctgat
    22201 gcacggcttg aaaggcgcgc ggacttggcg gcgtatgctt tccgacgcaa cgctcttgaa
    22261 agacaacgac ggcagcctga ttctcgaagc gtggaaagag gtcgaacggg caaatacgcg
    22321 cgaatagggc ggggctgtat gtgtgaaatg ccgtctgaag gcttcagacg gcatttgtgc
    22381 gtttgtcggg cggtgtttag ggggtggtaa tggcgtgttt cggcactttg tccatatccc
    22441 agtgtgccac cgcccagtcg agcagttcgg cagggcggtc ggcttccggt gcttcgggca
    22501 ggttgaggta acggaacact tggcggagga gttgttcgcg gcggtttaaa tccaatgcgg
    22561 gtgcgagcgt ctgtttcgac catttctgcc cttgtgcgtt ggtcagcagc ggcaggtggg
    22621 catattgcgg tgtcggaacg tccaaacact gctgcaaata gatttggcgc ggcgtagaaa
    22681 cgagcaggtc ttgtccgcgg acgatgtggg taacgccctg ttcggcatcg tcggcaacga
    22741 cggcgagctg gtatgcccag taaccgtctg cacgaagcag gacgaaatcg ccgatgtcgc
    22801 gggcgaggtt ttgggcgtaa ccgccgacga tgccgtctga aaagccgatg tcgcggtcgg
    22861 ggatgcggat gcgccacgca ggctgtttgc cttgcggtgc agggcgttgc tgcggattgc
    22921 ggcaacgtcc gttatagacg aatccgtctg cgccgtggac tgccgcagcc tgccagtctt
    22981 tgcggctgca atggcaggga tagaccagtc cggcggtttt cagacggcat agggtttctt
    23041 catacagggc gtaacggcgg ctctgatagg cgacttctcc gtcccactcg aatccgaatg
    23101 cctcaagcgt gtgcaggata tggcttgccg cccccggcat ttcgcgcggc ggatcgaggt
    23161 cttccatgcg gaccagccat ttgccgccgt ttgagcgtgc atcggcatag gaagcggcgg
    23221 cggtcagcag cgagccgatg tggagcagcc cggtcgggct gggggcgaaa cgtcctgtgt
    23281 acatatccgg tacggcccct ttatttaaga ctattaatca aagccattat ctcatcttta
    23341 ttcagttcca tcccgggttc ttcaagtaag gttaaatcat atagggcatt atattgctct
    23401 tcggtagctg aaccatccat aaggccggac gagaaaaaat cgaaggctct atctgcgatt
    23461 ctctcattac ttgcatttct actaaccagt ttcatcaatt ctgtatatat tgtatgggga
    23521 ggtaccggat ccttaaaaaa tcagatctga ataaatttca ttgggtgaaa tgatttcgag
    23581 tgcttttgcc atgattctat ttccttttgt gttggtgggt aatgtcgtgc attaacttct
    23641 tgcccattaa tatttttagg gtgaatcctt gatatgccgc actgtgtccg gtcaaacggg
    23701 cgatgccgtc tgaaagcctt tcagacggca tcggggatga aaagtttaaa ccaacggtgc
    23761 gagcagtttt tcaaaggctt cttcaaattg tttcaaaccg tcttcctgca aacgcgccgc
    23821 caaggtttcg acatcgatgc cgagcgcggc aatttcggca agctgcgctt gcgcttcgtc
    23881 cgcgccttcg gtcagcgtgg ctttggctgt gccgtggtcg ataaaggctt tgagcgtggc
    23941 atcgggaacg gtgttgacgg tgtgcgcgcc gatcaggctg tcaacgtaga gcgtgtcggg
    24001 ataggctggg tttttcacgc cggtagatgc ccataaaagc tgcacgcggt ttgcgccttg
    24061 ggcttccagt gcggcaaatt cgggggcggt gaaatattgt tcccaatctt gataggcggc
    24121 tttggcaagg gcgatggcga ttttgccttt gagccggtcg ggcagtgttg tgtccagcgc
    24181 actgtccaca cgcgagatga agaagctggc gacgacgtgg atatgggcaa cgctttgtcc
    24241 ggctgccaag cgtttggcaa tgccgcgcgc gtaggcggcg taggctttga gggtttgggc
    24301 gcgtgagaac agcagggtca ggttcacgct gatgccgtct gaaacgaggg tttcgagcgc
    24361 atcgatgcct gcgtcggtgg caggcacttt aatcatcgcg tttttgcacc cgatggcggc
    24421 gtagaggcgg cgcgcttctt caaccgtgcc ttgcgcgtct ttggacaatt cgggcgaaac
    24481 ttcgaggctg acgaagccgg ttttgccgcc ggtggattcg tgttcggcaa gacagacgcc
    24541 gcaggcggca cgcacatcgg caaccgccat cgtttcgtag cgttgtttgg gggtcaggtc
    24601 ttgctgcttg agggcggcaa cctcgtcggc gtaaagcgca tcgccggcga aggctttttg
    24661 aaagatggcg ggattggaag ttacgccgca cacgccttgt ttcagcattt gcgccaattc
    24721 gccgctttgc acgagcgagc gggaaaggtt gtccagccag atttgttgtc ctaatgcttt
    24781 aacgtccgat aaaatagtca tttctgattc ctttggatgg atgtgtatgt ttcaaaggct
    24841 tatgctaccc cgattcggaa attttgggta gttttattac agcaaaggcg gatggcgatg
    24901 gcaggaaacg aaaaatatct tgattgggca cgcgaagtgt tgcacaccga agcggaaggc
    24961 ttgcgcgaaa ttgcggcgga tttggacgaa aacttcgccc ttgcggcgga cgcgttgttg
    25021 cactgcaaag gcagggtcgt tatcacgggc atgggcaagt cgggacatat cgggcgcaaa
    25081 atggcggcaa ccatggcctc gaccggcacg cccgcgtttt tcgtccaccc tgcggaagcg
    25141 gcacacggcg atttgggcat gattgtggac aacgacgtgg tcgtcgcgat ttccaattcc
    25201 ggtgaaagcg acgaaatcgc cgccatcatc cccgcgctca aacgcaaaga tatcacgctt
    25261 gtctgcatca ccgcccgccc cgattcaacc atggcgcgcc atgccgacat ccacatcacg
    25321 gcgtcggttt ccaaagaagc ctgcccgctg gggcttgccc cgaccaccag caccaccgcc
    25381 gttatggctt tgggcgatgc gttggcggtt gtcctgctgc gcgcccgcgc gttcacgccc
    25441 gacgacttcg ccttgagcca ccctgccggc agcctcggca aacgcctact tttgcgcgtt
    25501 gccgacatta tgcacaaagg cggcggcctg cctgccgtcc gactcggcac gcccttgaaa
    25561 gaagccatcg tcagcatgag tgaaaaaggg ctgggcatgt tggcggtaac ggacgggcaa
    25621 ggccgtctga aaggcgtatt caccgacggc gatttgcgcc gcctgtttca agaatgcgac
    25681 aattttaccg gtctttcgat agacgaagtc atgcatacgc atcctaaaac catctccgcc
    25741 gaacgtctcg ccaccgaagc cctgaaagtc atgcaggcaa accatgtgaa cgggcttctg
    25801 gttaccgatg cagatggcgt gctgatcggc gcgctgaata tgcacgacct tttggcggcg
    25861 cgg
//
LOCUS       DAWKQB010000012        24616 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.012, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000012 DAWKQB010000000
VERSION     DAWKQB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24616
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.012"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..194
                     /locus_tag="WJL14_000384"
     CDS             33..194
                     /locus_tag="WJL14_000384"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655511.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            244..528
                     /locus_tag="WJL14_000385"
     CDS             244..528
                     /locus_tag="WJL14_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655512.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWARGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(653..1369)
                     /gene="trmB"
                     /locus_tag="WJL14_000386"
     CDS             complement(653..1369)
                     /gene="trmB"
                     /locus_tag="WJL14_000386"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ7655513.1"
                     /translation="MTDTAENQTPNERQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDARFGSSRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIDENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1491..2894)
                     /locus_tag="WJL14_000387"
     CDS             complement(1491..2894)
                     /locus_tag="WJL14_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7655514.1"
                     /translation="MNNPDLPYRQALECLSQKQYNFTEVRRLLTEAFSAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLIPLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYRKAAAQGLAAAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDEAVHWFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDIYRYGLGMTSDKEKALHYYRQAAEAGSLAAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAERLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
     gene            complement(3167..5053)
                     /gene="uvrC"
                     /locus_tag="WJL14_000388"
     CDS             complement(3167..5053)
                     /gene="uvrC"
                     /locus_tag="WJL14_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ7655515.1"
                     /translation="MPSEAVFQIIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSNQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGIAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     EHLH"
     gene            complement(5108..7285)
                     /locus_tag="WJL14_000389"
     CDS             complement(5108..7285)
                     /locus_tag="WJL14_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ7655516.1"
                     /translation="MQQKVRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSVYMLFFSPHAAYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRDGEWRQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAI
                     MVGIGKAVKHGIWFKDAAAIEEAAHVDAVVLDKTGTLTEGSPQVAAVYCVPDSGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLDIPAAQNAQTVVGAGITAEVEGVGLVKA
                     GKAEFAELALPKFLDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLRNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            7424..8374
                     /gene="trxB"
                     /locus_tag="WJL14_000390"
     CDS             7424..8374
                     /gene="trxB"
                     /locus_tag="WJL14_000390"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ7655517.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGTELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            8530..8898
                     /gene="rpsF"
                     /locus_tag="WJL14_000391"
     CDS             8530..8898
                     /gene="rpsF"
                     /locus_tag="WJL14_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ7655518.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            8899..9201
                     /gene="priB"
                     /locus_tag="WJL14_000392"
     CDS             8899..9201
                     /gene="priB"
                     /locus_tag="WJL14_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ7655519.1"
                     /translation="MGFNNLVSLAVLIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            9208..9438
                     /locus_tag="WJL14_000393"
     CDS             9208..9438
                     /locus_tag="WJL14_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ7655520.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            9455..9907
                     /locus_tag="WJL14_000394"
     CDS             9455..9907
                     /locus_tag="WJL14_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ7655521.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTITVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(10695..11501)
                     /locus_tag="WJL14_000395"
     CDS             complement(10695..11501)
                     /locus_tag="WJL14_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7655522.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFTMMPGMVFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(11502..12248)
                     /gene="pssA"
                     /locus_tag="WJL14_000396"
     CDS             complement(11502..12248)
                     /gene="pssA"
                     /locus_tag="WJL14_000396"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ7655523.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            12831..13313
                     /locus_tag="WJL14_000397"
     CDS             12831..13313
                     /locus_tag="WJL14_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ7655524.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNANDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVYQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(13365..14576)
                     /locus_tag="WJL14_000398"
     CDS             complement(13365..14576)
                     /locus_tag="WJL14_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ7655525.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFSSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTEKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGIKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            14799..17237
                     /locus_tag="WJL14_000399"
     CDS             14799..17237
                     /locus_tag="WJL14_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ7655526.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLVISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHHVVAFVKEQAPANYVEGLLTGEAAQETAN
                     IVSPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPSD
                     LNGSRKILAHKDHL"
     gene            17445..18755
                     /locus_tag="WJL14_000400"
     CDS             17445..18755
                     /locus_tag="WJL14_000400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ7655527.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVKKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            18851..19465
                     /gene="clpP"
                     /locus_tag="WJL14_000401"
     CDS             18851..19465
                     /gene="clpP"
                     /locus_tag="WJL14_000401"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ7655528.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQVLENRASLQL"
     gene            complement(19518..20783)
                     /gene="ispG"
                     /locus_tag="WJL14_000402"
                     /gene_synonym="gcpE"
     CDS             complement(19518..20783)
                     /gene="ispG"
                     /locus_tag="WJL14_000402"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ7655529.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(20799..21560)
                     /gene="pilW"
                     /locus_tag="WJL14_000403"
     CDS             complement(20799..21560)
                     /gene="pilW"
                     /locus_tag="WJL14_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ7655530.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(21563..22657)
                     /gene="rlmN"
                     /locus_tag="WJL14_000404"
     CDS             complement(21563..22657)
                     /gene="rlmN"
                     /locus_tag="WJL14_000404"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ7655531.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(22801..23226)
                     /gene="ndk"
                     /locus_tag="WJL14_000405"
     CDS             complement(22801..23226)
                     /gene="ndk"
                     /locus_tag="WJL14_000405"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ7655532.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(23285..24436)
                     /locus_tag="WJL14_000406"
     CDS             complement(23285..24436)
                     /locus_tag="WJL14_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ7655533.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         5831 a         5967 c         6774 g         6044 t
ORIGIN      
        1 aagattttat acgggctacg gcttgctggc ttatgcgttg gcttaatttg aaaaacagca
       61 catcggttca taatacacaa ggtattagaa caaaagaagc agttgaacag ggattgcgtg
      121 gtaataatag acctgaaaaa ggaataggta atagatggaa ttatgatgga tttataaagg
      181 aattagcgaa gtaacaggta ttccaaatat ttctgataaa tataaagaac aaggagtaaa
      241 aaaatgaaag taatcactat tacattgttt actagctgca tactatctgg atgctatggg
      301 cttgaatatg gaatgagtgg cggcattcct catgatgact gggcaagagg tagccatgtt
      361 agtcaagcca aagcagatgc tgcgtggaaa gaatgttctg acaaatatat gagaaatggt
      421 ggttcaggat tagtagaaca atgtatgaat gataaaggat taatacgttt atctgaaata
      481 cgtcgtaggc aaaagagagc agaaacacaa agagcagtaa ttaagtaaca ataatgaaat
      541 actactgttc ccgaacaaag cggcatttac gcaggtgatg acggctatat tttgaaaaat
      601 gccgtctgta agcaaacgtg cttcagacgg cattttttta tccggcggtt tgttatccga
      661 tccgtttgaa taccaagtcc caaacgccgt gtccgaggcg tttgccgcgc gcttcgaatt
      721 tggtttcggg gcggtagtcc ggggtggggg cgtagtctgc cgccgtattt tgcaggctgt
      781 cgaagctgct gaggacttca agcatctgct gtgcatattc ttcccagtct gtcgccaggt
      841 ggatatagcc gccggttttg agtttgggca gcagtttggc gatgaagggg gcttgtatca
      901 gacggcgttt gtggtggcgt tttttgtgcc acgggtcggg gaagaatatg tggatgccgt
      961 cgagcgagcc gtcttgcagc atattttcga caacttctac ggcatcgtgc cgcatcacgc
     1021 ggatgttttc caaatggttt tcgtctatga gtttgagcag gttgcctacg ccgggaccgt
     1081 gtacgtcgat ggcgagaaag tcggtttcgg gcagcctgcg ggcaatttct gcggttgccg
     1141 tccccatacc gaagcctatt tcgaggattt tgggtcggct gcttccgaaa cgggcatcaa
     1201 gatcggccgg tgttgcttgg tagtcgatgc cgaagctgtc ccataaggta tcgatggcgc
     1261 gttgctgcgc ggcggtcata tggctttggc ggaggacgaa gctgcggatg ctgcgggggt
     1321 gtcctgcttg acgctcgttt ggtgtttggt tttcggcggt gtctgtcata atgctttcat
     1381 gcgcttgtgc aatcaggatg ccgtctgaat gtgaaatgtc tgttcggacg gcgggaaggg
     1441 aaaggggcga ttatacctgt ttcgtttcgg cagggtaggt gtaacgctgt tcagacggca
     1501 ttttgccact gttgcaacag ttcttgcagg acgctttttt gcccgtatcc gttgtttatg
     1561 gcttcttgca ggtagcggca ggctttttct ttgtccggcc cgacaccgtg tccgctgtaa
     1621 tggatgcagg cgaggttgta gaacgccata ctgtcttttt ttgcggcggc tttttcaaac
     1681 catttgcgtg cttcattgta gtcggcggtc ataccctgtc cgaagtaata catgctgccc
     1741 agattggttt gggctttgct gtgtccgagt tctgcggctt ctgtgtagag tttgagcgcg
     1801 gctgcgtatt cgggcgcgca ttgtaatccg taatgcaggg cttgggcttt ttgataaagc
     1861 cgttctgccc tttgccgcct gatggcggaa tctttaatgc cgccatattg gtcgggatgg
     1921 tttaacgcgc tgtcggatat gagtttctga tacgcggcaa ggctgccggc ttcggctgcc
     1981 tgccggtaat aatgcagggc tttttcttta tcggaagtca tgcccagtcc gtagcggtag
     2041 atgtcgccca gtatccgcaa accttcggga tggcgttcgg cggcggctgc ttcggcgtga
     2101 tgcagggcaa gtttgtagtt tctttccgga tgcttgcctt gcaggtagat gtcggcaagt
     2161 gcggcatggg catcggggtc attttcctgt gccgcctgtt gaaaccagtg aacggcttca
     2221 tcaggagccg taggtaggat gagctcggca agcggggtgt aggcgggaat gtatccttgt
     2281 tgtgcggcgg cgcgcaaatg attgacggcc tgtgccgtgt cgactttcgt cccttgtccg
     2341 aaacggtatt gcagcccgag ctgccagtgt gccgccgcca agccttgtgc cgccgctttg
     2401 cggtagagca ggtgcgccgt ttcaaaatcc ggcggcgtgc cgtaccggca gtattgggca
     2461 agcaggtatt gcccgtgggg gttgttgttt tttgcgcttg tttttgccca tttcagggct
     2521 tcttcaaaat gtcgggacgc atacagaagg tacatcaggc ggacttgtgc ttcaggcagt
     2581 ccttcctctg ccaacggtct gtaaagcggt atcagggttt ccgggggcgc gccttccaat
     2641 tcttggatat atgccagatt gtaacgcgca tagggatgcc cctgttcagc ggcgatgcgg
     2701 agcatttcca taccttgttc gcggtcttgg taggggctgt ccgcgtccat caggtgtttt
     2761 gccaactcga atgcggcggc gggatgacct gccgagaacg cttctgtcag cagtcggcgg
     2821 acttcggtaa agttatattg tttttgagac aggcattcta aggcctgccg atagggtaag
     2881 tcgggattgt tcatgtcaga tcgttttccg ttttgtcgaa tcatcgttat tgtgttatga
     2941 agttaggttt atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     3001 aaatagtacg aaaccgattc acttggtgct tgagcacctt agagaatcgt tctctttgag
     3061 ctaaggcgag gcaacgccgt actggtttaa atttaatcca ctatatatcg gtataccgac
     3121 ctgctgtatc atatttttta cagacggttt ttgcttgggc agcatactaa tgcagatgct
     3181 cgtaaatggt ttccgccaat gccttgctga tgccttccac tttttccaag tcctcgcggc
     3241 tggcggcaat cacgccgcgc agaccgccga agcgggtgag cagggcttgg cggcgtttgc
     3301 tgcctacgcc ggggatgtcg cttaaggagg aggtaacgcg ggctttgtcg cgttttttgc
     3361 ggtggccggt aatggcgaaa cggtgcgatt catcgcgtac ggtttgcaat agatgcaagg
     3421 ccgggctgtt gggcggcagg cggaagactt cgccggtaaa aggcagtatg agttcttcca
     3481 taccggcttt gcgttcgggg cctttggcga taccgaccaa aggaatgtgc agcccgagtt
     3541 cttcccatac cgatacggcg atgccgattt gccctttgcc gccgtcaatt aacacggcat
     3601 ccggccattt gacggtctcg ccgttggctt cggcttcctg cattttgccg taacggcgcg
     3661 tcaacacttc gcgcatggcg gcgtagtcgt cgccgggttt ggcggtcgtg atgttgtagc
     3721 ggcggtattg cgagggctgg atgttttgct catcgtacac gacgcaggag gcgatggtgg
     3781 cttcgccttg ggtgtggctg atgtcgaagc attcgaggcg gttgaggccg tctgaatcca
     3841 taccgaggat tttggcgagt tcgtcgatgc ggtgttgctg gttgctttgt tgcaggcggc
     3901 gttgtgcaat cgccatttgt gcgttttgtt ccgccatttt caaccaaact ttgcgttcgc
     3961 ctatggtttt ggttacgaac tgcatttgtt tgccgtgttc gccttccaaa gcctctttca
     4021 gcgcatcggg gacggggaag ttgttgatga taatgtcggg tttgcttttg cccagatagt
     4081 gttgggcgac gaaggcttcg gcatagtctt gcccgtttgg ctcgggatcg tttttggtgt
     4141 cggggaagaa acttttgtct ccgacgtgcc gcccaccgcg tatgctgacc cagtgtacgc
     4201 agacgatgcc gtctgaaacc gccagcgcga gcaaatcgat atcgttgggg ttgttcgggt
     4261 ttttgctgtc gatgaactgg ttgctctgca taatgccgag tgcttggatt tggtcgcggt
     4321 agcgggcggc ttcttcaaat tgcagattcg cggcggcagt ttgcattttg tgttgcaggg
     4381 tgcgggtcag ttcgtcggtt ttgccgttga gaaaagtggc ggcttgacgc acgctgtcgc
     4441 ggtagtcttc ttcgctgatg tgtccgacgc agggcgcggt gcagcgtttg atttgataca
     4501 gcaggcaagg gcggtcacga tgctcgaata cgctgtcttc gcaggtacgc agcataaaga
     4561 ctttttgcaa cacttgaatg ctgtcgcgca cggcgttgct gttgggatac gggccgaagt
     4621 attggtttgg ctttttcagc gtgccgcggt aatacgccat ctgcggatat tgatggccgc
     4681 tgagcatcaa ataaggatag cttttgtcgt cgcggaaaag gatattgtat ttcggcgaca
     4741 aggctttgat gaggttgttt tcgagaatca gggcttcggc ttcggaacgc gtgatggtgg
     4801 tttcgatatg atgaacctgt tttaccatca atgcgatgcg cggcgaatgg tcgttttttt
     4861 ggaaatagcc ggacacgcgc cgcttgaggt tgaccgcttt gccgacgtat aagactttgc
     4921 cgccttcgtc aaaaaaacgg tacacgcccg gcagcttggg caggtttttg aggaagagcg
     4981 ggatatcgaa ttgttccgga gaacggtttt ctgtattcat atcaattatt tgaaaaacgg
     5041 cttcagacgg catatcggca gcggcagcct tatccgttcg gaaaagctgt cgggtggtta
     5101 tatgcggtca atcgattttt acccgtttca ggcgcaaggc gttgctcaac acggaaaccg
     5161 agcttgccgc cattgccgcg cctgcgatga cagggtttaa aaaaccgagc gcggcaagcg
     5221 gaatgcccaa aatattgtag aagaaggcga aaaacaggtt ttgcttgatg tttctcaacg
     5281 tcgcccgcga taccgatagg gcatcggcga gttggttgac cgaatgttgc atcagcgtgg
     5341 cggatgcggt gtgttcggca acgtccgcgc cgcctttcat ggcgaagcta acgttggcgg
     5401 cggcgagcgc aggtgcgtcg ttgatgccgt ctccgaccat cgccacggtt ttgccggcgg
     5461 ctttgagttt ctgcacttcg gcggctttgt cgcgcggact catattaccg aaggcgtgtg
     5521 cgatgcccag ttgtttggcg acgtattcga ccgtgccttg gttgtcgccg ctcataatat
     5581 agacatcgat attgtgtttt ttcagacggc ctatggcttc ggcggtatcg gctttcaacg
     5641 cgtcggcaag tgcgaatgcg ccgatgggtt tgttatcgac tgagaccgca acaatgcttg
     5701 caatatccca aacgccgtct aaaaacttcg gcaaggccag ttcggcaaat tcggctttgc
     5761 ctgctttcac caaacccacg ccttccactt cggcggtaat gcctgcgccg acaacggttt
     5821 gtgcgttttg tgcggcggga atgtccaaac cgcgcgcttg ggcggcggag acgatggcac
     5881 gggcgagcgg atgggcggcg ttttgttcga cggcggcggc gatgcggtac aaagcgtctt
     5941 cgtcaaagcc gctgtcggga acgcaataaa cggcggcaac ctgcgggctg ccttcggtca
     6001 gcgtaccggt tttgtccaac acgacggcat cgacgtgggc ggcttcctct attgctgccg
     6061 cgtctttaaa ccaaataccg tgtttcaccg ctttgccaat gccgaccata atcgcggcgg
     6121 gtgtagccag accgagcgca cacgggcagg caatcaccaa aacggcgacg gcgtgcatca
     6181 gcgcaaccgt ccaatcgccc ttaatcagcc aagtaacaat aaaagtcaac aacgcgatgc
     6241 ccacgacggc aggcacgaac accgccgccg ccttgtccgc cacgcgcgca atcggtgctt
     6301 tactgccttg cgcttcagag agtgcgttca tcatgtcgcc gagcagggtt tggctgccga
     6361 gctgcgcggc gcggtacacc acgctgcctt cagtcatcag cgcgcccgcc aatactttgc
     6421 cgcctgcctt tttctcttcg ggattggatt cgccggtaag atggctttcg tccgcccagc
     6481 cgctgccgct ttctatgatg ccgtcggcgg caatgcgttc gccgtgattg gcgcggatta
     6541 ggtcgccgat ttgcacttgg tcgatgggta gctgccgcca ttcgccatcg cgttgcacgt
     6601 tgacttgggt tggcgtgagt ttgagcagca agcccaagct gttcaggctg gattttttgg
     6661 tgcggtgttc caaaaattta cccagtgaca caaaaccaat caccattatg cctacttcaa
     6721 aatacacatg cgccataccg tacgccgcgt gcgggctgaa aaacagcata tagacggaat
     6781 acaggtaaat cgagacagtg ccgatggtaa cgagtacgtc catattcgcc agcccgcctt
     6841 taatgctcgc ccacgcgctt ttgtaaaatg gcaccgccag ccaaagctgc accacactcg
     6901 ccaatgcaaa ctgcaacaag ggcgacaaca tccaatcatg ccgtgtccaa ttcagccctt
     6961 ttagcatcat ccctaccata ccgataagga acgggatatt gatggccagc aaaagccaca
     7021 acctccagcc gatatggtgt tctgcttcgg gttgcggcaa tgtatcttcc gttttttcct
     7081 tcgcgccgta accggttttc tcaatgattt tggcaatgtc ggctactgag gttttgctgt
     7141 cgtcaaacac tacctgcgcc tcttcgctgg cgaagtttac ccccgccgat tcgacaaaat
     7201 cttttttgtt caacactttt tcaatgcgcg aagcacatgc ctggcaggtc atgccttcga
     7261 tttggaaacg gactttttgt tgcataagcg tgattcctgc tttgatattc agacggcggt
     7321 caaggctgcg tgatgccgtc tgaacgccgg tatggtcgaa atttctcaaa cgcgccgata
     7381 attcgagtag aatccgaaaa cttgacacag caaaggaaaa acaatgtccc aacaccgcaa
     7441 actgattatt ttgggttccg gccccgccgg atacaccgcc gccgtctatg ccgcgcgtgc
     7501 caatttaaac cccgtcatta ttacaggtat cgcgcaaggc gggcaactga tgaccaccac
     7561 cgaagtggac aactggcctg ccgatgccga cggcgtgcaa gggacggaat tgatggcgcg
     7621 gtttctcgcc cacgccgaac gtttcggaac ggaaatcatt tttgaccaaa tcaacgccgt
     7681 cgatctgcaa aaacgcccgt ttacactcaa aggcgatatg ggcgagtaca cttgcgatgc
     7741 cctgattgtc gcaacaggcg cgtccgccaa atacctcggt ttgccgagtg aggaagcgtt
     7801 tgcaggaaaa ggcgtttccg cttgtgccac ctgcgacggt ttcttctaca aaaaccaaga
     7861 tgttgccgta gttggcggcg gcaatacggc agttgaagag gcactctacc ttgccaatat
     7921 cgccaaaacc gttaccctga tccaccgccg cagcgagttc cgtgccgaaa aaatcatgat
     7981 cgacaaactg atgaaacgcg tggaagaggg caaaatcatc ctcaagctgg aaagcaacct
     8041 gcaagaagta ctgggcgacg atcggggcgt aaacggcgca ttattaaaaa acaacgacgg
     8101 ttctgagcaa caaattgccg tcagcggcat ttttatcgcc atcgggcaca agccgaatac
     8161 cgatattttc aaaggtcagt tggaaatgga cgaagccggt tacctgaaaa ccaaaggcgg
     8221 cacggcggac aatgtcggcg caaccaatat cgaaggcgta tgggcggcgg gcgacgtaaa
     8281 agaccatacc taccgtcagg cgattaccag cgcggcttcc ggctgtcagg cggctttgga
     8341 cgcggaacgc tggctgggca gccaaaacat ttgaatgccg tctgaaaccg aaattttcgg
     8401 cggaatgcaa atttttcgca ccaaatcctt gattccgtcg gcaaacccca atataatgcc
     8461 tgctctttga accttgcctt ctgctttcgc atggcagaag gctgttttta ccatccataa
     8521 ggagataaca tgcgtcatta cgagatcgtg tttatcgttc atcctgatca aagcgagcaa
     8581 gtgcctgcta tggttgaacg ttacaaaacc atgattgctg aagcaaacgg taagattcac
     8641 cgtttggaag attggggtcg ccgccaactg gcttacccga ttaacaaaat ccataaagcc
     8701 cattacgttt tgatgaacat cgaaaccact cccgaagtgg ttgaagagct ggaaaccgca
     8761 ttccgcttca atgatgccgt attgcgccat ctaaccatca aaaccaaaca cgccgttacc
     8821 gaagcatccc ctatgttggg tggtgaaaag gctaagaacc tgttgagcgg cgcgtctgaa
     8881 gaagcggtcg cccaataatt gggattcaat aatcttgttt cgcttgccgt gttaattgaa
     8941 aaggttttcc ctattcgata tacgcctgcc ggaatccctg ttttagatat tattttaaag
     9001 cacgaatcgt ggcaggagga aaacgggcag caatgccttg tccaattgga aattccggca
     9061 cggattttag gcaggcaggc ggaagagtgg cagtatcggc aaggtgtata tgttcacgtc
     9121 gaaggttttt tagctcaaaa aagcagacgt tcccttatgc cgatgctcag gatacaaaat
     9181 attcaagaat ataaaggtta aacgacaatg gctcgtcaat cattcaaacg tagaaaattc
     9241 tgccgtttca cggctgaaaa aatccaagaa gtcgattaca aacaagttga tttgctgaaa
     9301 gactttatct ctgaaaacgg taaaatcatt cctgcacgca tcacaggaac gaaggcattc
     9361 taccaacgcc aattggctgt tgccgtaaaa cgcgcacgct tcctggctct gctgccttac
     9421 accgaccaac acaaataatt ttggagattg aatcatgcaa attattctgt tagaaaaaat
     9481 cggcggtctg ggcaacttgg gcgacatcgt aaccgttaaa aacggctacg cccgcaactt
     9541 tctaattccc gcaggtaagg caaaacgtgc gaccgaagcg aatatgaaag agtttgaagc
     9601 acgccgtgcc gaattggaag ccaaacaggc tgaaattttg gcagatgccc gagtccgtca
     9661 ggaaaaattg gacggtcaaa ccattaccgt tgcacaaaaa gccggtgtgg acggtcgcct
     9721 gttcggttcc gtcaccaatg ctgatattgc ggctgcaatc gttgctgccg gcatcgaagc
     9781 cgtgaaagca aatgtacgtc tgccgaacgg tcctctgaaa gccgttggcg agtacgaagt
     9841 ggaagtggct ttgcacactg atgccgttgc taaaattacc gttgccgtcg ttgccgcaac
     9901 cgagtaattg aaattatcag atgccgtctg aaaccctgcc gattgtcgga atgtggtttc
     9961 agacggtatt tgttttgttt ttcgtatatt ctgcaaaaat cgggatggtt gtttttatag
    10021 tggattaaat ttaaaccagt acagcgtacc tggcttcaat atactcaata ggataggtct
    10081 cttcctaacc gaaaaaataa ttgtcttttc ccttgcaaat aattgaaatc aacagattac
    10141 taaaacacag ccaaaatatt caattgacaa aaacttttct gattttgaga aatctgtgaa
    10201 aatcagggga ttatttattt tgaaaatggt atcgggcata aaatttggct gtgtttcaat
    10261 atggtgctga taacatactt tgtttcaaaa tgttcaatag gataacttcc tgattgaccg
    10321 aaaaataatg gccttttccc ttgcaagcag ttgaaatcaa cagattgtca aaatacatcc
    10381 taaaaattta ataacttata tatatgattg caaaggttac ggcttgtatg ccggtcatta
    10441 tggcggaaat accgtttggg aggcagccgc atcgcgtgga atcggcacgg caggcgatgc
    10501 gccgatgcct tcgggcgtga aggcggggca atcggcagtt tggaaaaagg caatggaatc
    10561 tgtgggcaga agaagttaag ggtaaaagtc gggcgcacgg tcaggttgtc tgtccttttg
    10621 tcggaacagg agactgggca gctttgtcct gattaattgc ttgtgcaaac ggtaaaaagg
    10681 ttttttcgtg tgttttaaag caggttgtac agcatttttc cggcaatcaa aagcaacata
    10741 atgccgaagg attttttgag tttggcagaa gaaagtttgt gggcggtttt gacaccgagc
    10801 ggggcaaagg caatggttgc cgcgctgagg acggcgacgg cgggcaggta aaggaagccc
    10861 agtgaccctt cgggcaatcc tgcaatattc aggccgttga gcagatacga tattgcgccg
    10921 gagagtgcaa tcggccaggc aaggccggat gatgtgccga tggctttatg ggcggggaag
    10981 ccgcagtgga ttaagaaggg gacggaaagt gaaccgccgc ctatgccgac ccagctcgac
    11041 attgtgccga acagtgtgga aaccgcagtc agtccgggca gtccgggcag cgggcgggat
    11101 gccgtctgag ggtcggtatg cagtgttttg aatgcgacgg cggttaaaaa caggatgaag
    11161 aaaatttgaa gcccgaacgc tgggatatat tttgcggaga gtgcgccagc gaatacgccg
    11221 aataccatac ccggcatcat cgtaaatacg gttttccagt cgaccgcctg ttttttgtgc
    11281 tgccccagca tactggaaaa ggcggtgaag accatgacgg cgaaggatgt gccgacggcg
    11341 aggtgttgcg cgtaaggatg ttgtgccaaa ccctgcaaat caagcaccca taaaacgaca
    11401 gggacaatca gcgtgccgcc gcctacgccg aacaggccgg caataaaacc tgccgcactg
    11461 cctacggcaa gcaggattaa gataatgtcc caatgccaca tttaatccgc ctttctgtat
    11521 tttttccaga atcggcgtgc cgccataatg tagccggaca ggctgtatcc gagaaagaac
    11581 aggaagagga cgagcgacgg ttcccaagtg accagaagca gcagtaagac ggcaagcagc
    11641 attccgacaa aggggacttg tctgcggatg ttgatttctt taaaactcca aaaagggatt
    11701 tggacaatca tcgacaggcc ggcaaacagt gtgatgccca atgcccacca gtggacggcg
    11761 gggaattttt cgacgctgtg gttgacccaa atcagcccga caatcagcgc ggcggcagtc
    11821 ggactgggca cgccgataaa ccagcgtttg tccaccttgc cgatgagcgt gttgaacagc
    11881 gcaaggcgca gggcggcgca ggcgcagtag atgaaggcga cggaataacc gattttgccg
    11941 aactgccaaa gctgccattt gtaggcaatc agagcgggag caacgccgaa gctgaccata
    12001 tcggcaaggc tgtcgagctg ctccccgaac gcgctttggc tgttggtcag ccgcgccacg
    12061 cgcccgtcca taccgtccag caacatagag atgaataccg cgatggcggc ggtttcataa
    12121 cgtccgtgca tggattgggt gattgcgtaa aatgcggaaa acagcgcggc gatggtaaag
    12181 gagttgggca gcaggtagat actgttttga cgcagggagc gttgcggggt gttggtcggg
    12241 gtttccatag ctgaagtgtc tcacggacgt ggagaatcga taggggatta taacccaaac
    12301 cattatcgaa caatacgccc tttaggatga agccctcgga ggcggtttgt ttttctgatg
    12361 gcggatgctt tgcggggcgg tttgactctg cctgccaccg ggtgtgttgt ttcggatttt
    12421 tgccggatgg atattgttgc cgggcatttc tgggcttgcc ggaaggtatc ggggggaggg
    12481 catcggaata tgcggcgcac cgttcaaacc ggggcggcgt ttccctattt tcagggggtt
    12541 gcaggtttca acaaattgaa agatattttt tgcatttccg accgtatgct gattttgaag
    12601 cgggataaat tataggacaa ggtttttcgc ccgcggcaag aattgcccgc aatgccgtct
    12661 gaaaacaacg gcttgccgct aaggcggcgg tatgcggatt tgcgctgccg aaatcggttt
    12721 gccatacccg gcggtgcaat ctgcggggag ggataagccg ataaggcggg acttatcctg
    12781 ttttttgttt ataatgcaac agaatttgga cgaaccgtcg ggaatttatc atgatgaaaa
    12841 agatactggc cgtatcggca ctatgcctga tgactgcggc ggcacgggct gccgatactt
    12901 acggctatct cgccgtttgg cagaatccgc agaatgcaaa cgatgttttg caggttaaaa
    12961 ccacaaaaga agattcgacg aaaagcgaag cgtttgccga gttggaagct ttctgcaaag
    13021 gtcaggacac gcttgcgggc attgccgaag acgagccgac cggatgccgg tcggtcgtgt
    13081 cgctgaacaa tacctgtgtc gcgctggcat acccgaaagc cttgggcgcg atgcgcgttg
    13141 aaaacgccgt tgtgattact tctccgcgtt ttacgagcgt ttatcaggtc gcactcaacc
    13201 agtgcatcaa aaaatacggc gcacagggac aatgcggctt ggaaacagtg tattgcacgt
    13261 cttcttctta ttacggggga actgtgcgct ctttgattca aaatctcaaa taaaacggga
    13321 aatgccgtct gaaagatgtt cagacggcat ttctatatcg acggtcagga ttctttcgga
    13381 tcgggcagca ggctgttcaa cataatggca agtacggcgc acaagcccac gccggcaaag
    13441 ctgaagctgc ccaatttgag cgtcatgccg ccgatgcctg tggtcagtac cgagctgacg
    13501 atgaccaggt ttttcggcag catcaaatcg actttggcat caatcagggt tttgatgcct
    13561 aaagaagcaa tcgtgccgaa cagcagcagc ataatgccgc ccattactgg catcggaatg
    13621 gaagccaaaa acgcattgaa tttgccgaaa aacgccatgc agacggcaaa aaccgccgcc
    13681 caagtcatga tgacggggtt gctgtttttg gtaatcatca ccgcacccgt tacttcgccg
    13741 taggtcgtaa ccggcgggcc gccgatcaga cccgcaacgc atacgcccaa accgtcgcct
    13801 gcaagggttt tgtccaagcc cgggtctttc gtatagtctt tctccgtcac attgccgatt
    13861 gccatgatgc cgccgatgtg ttcgatggcg ggggcgacgg caacgggcag cataaacagt
    13921 gcagcctgcc agttgatctg aggcgtttca aaatggggaa cggcgaacca gggcgcgtgt
    13981 gcaatgcttg ccgtgtccac cagtcccatc agcagtgcca aaacataacc cgaagcgaca
    14041 ccgatcaaga tgggaatcag cttcatcatc ctgctgctga aaaccgatac gatggcggta
    14101 acggcaaagg taaagccgga aagaatcagc gaatcggtat agtcgatgac ctgtttgccg
    14161 tccgcctgac ccattgccat gctgcttgcc gccacggcaa cagacagacc gatgaccatg
    14221 atgacggggc cgatgacgac cggaggcagc agtttgtgta ccgctgccag tccgcgccaa
    14281 cggatcagcg cggcaaacac aaaatacata aagccggcgg caaacagtcc gaacatggtg
    14341 gaaggcagcc cccattcgcc gacggagtag ataatcggtg cgataaaggc aaacgaagaa
    14401 ccaagaaaaa tcggcacttt gcgtttggtt gtgatttgga acagcagcgt tcccaagcct
    14461 gcgcccaaaa gcgcaagagc cggattcaga ccggtcagca ggggaaccag caccattgcg
    14521 ccgaatgcca caaataaaat ctgcgcgccg gaaacggcaa gtttcagttg gttcatacca
    14581 tcttctttca atcggttcaa acggcacgga ttatactggt ttgccgaggg tctgcgtaaa
    14641 aatacatatg gcgggcaaca gatatgccgt ctgaaaggca atgtctgtct gttgcaggat
    14701 gaaagcagga gaaatggagt ataatccgac actttccata ttcaatccgc aagccgtccg
    14761 gcttccttat ttcagacggc attttccggt aatacggcat gacagaaaaa tcccataaaa
    14821 aaacagcaaa aggcagggcg ggaagcccgt ccccaacgtc cgcacgcaac aagaaagccg
    14881 acaacggcgc acggggcaat aaagtttccg agcggctcaa ggcggtcaaa gagttgcaga
    14941 aaaccgaaac caaaaaggcg cgtcccgaac atgtcgtcaa ccttatcggc gacgcactgt
    15001 ggctgatggg tttggcggca accctgtatt tggtgatttc cctgatcagt ttcgatatgg
    15061 gcgatccgtc ttggtcgcac agttcgccgg ttgtggaaga tgtcgccaat tggggcggac
    15121 tgttcggcgc gtatgttgcc gatgtcggct attatctttt cggctggtcg ttctggtggt
    15181 ggatagcggc tgcctgcgtc atgctgtata aaaatttccg cctgcacgca aaacagacgg
    15241 aaaacgaggc atacaaccac aaaatcgctg ccgccgcgct gtttgtcctg acggtcttca
    15301 gccccgtctt ggagtatttt gtgctgggcg gaaaatatgc cgactccctg cctgtcggag
    15361 caggcggtat ggtcggcata cgcgtcggcg cagtgtttgc gtggctgctg gggaaatcgg
    15421 gcagcctgct gattatcttg gttgttctgc tgttgtcgtt gtccctactg gtgcagattt
    15481 catggctgga atttttgaac ggtgcgggca gggcggttca aaaccgcctg agtgccttat
    15541 ccggcaaggt catggcttta ggaaaacgcc ggccgaatac caaaacagac ggtgtcgata
    15601 cccaaaatac acggcgcatg gtaaaagaag ccaagaatat tacggccaaa cccgttgcct
    15661 tgcccgaagg cagcagcagc aaccgcaaat ccgtcgcggt ttccgtcgcg ccgccgccca
    15721 aaattcaggt ttctctgttt gaagatgacg aacctcggca ggcgggcgaa taccacaagc
    15781 ctacattgaa cctattgcgg attcctgaca gcgaacccgt cagcatcaat cccgccgaat
    15841 tggagcgcac tgccgaactg atcgaatcca aactggcaga attcggcatc ggcgtacaag
    15901 tcgtatccgc cacatccggc cccgtcatca cgcgctacga aatcgaaccc gcgcaaggtg
    15961 ttaaaggcag ccaaattgtt gccttgtcga aagatttggc acgctctatg tcgctgcaat
    16021 ccgtgcgtat cgtcgaaacc atcgcaggta aaaacacgat gggcatcgag ttgcccaacg
    16081 acaaacgcca agacgtgatg ttgagcgaaa tcttgtcctc gcccgtgttt gccgaagcca
    16141 aatccaagct gaccgtcgcg ctgggcaaag acattgccgg tacccccgtt gtcggcgact
    16201 tggcgaaaat gccgcacctt ttggtcgccg gtatgactgg ttcgggcaag tccgtcggcg
    16261 tgaacggcat gattatgtct atgcttttca aagctacgcc cgacgaagtc cgcttcatta
    16321 tgatagaccc gaaaatgctc gagttgagca tttacgacgg cattccgcac ctgctctgtc
    16381 ccgtcgtgac cgatatgcgc gaagcagggc aggcgttgaa ctggtgcgtc gccgaaatgg
    16441 aaaaacgcta ccgcctgctt tcccacgccg gtgtacgcaa tctggaaggc ttcaaccaaa
    16501 aagtcgaagc cgcaaaagcg gcaggcaagc cgctgctcaa tccgttcagt ctgaaccccg
    16561 acaaccccga gcctttggaa aaactgccga tgattgtggt cgttatcgac gaacttgccg
    16621 acctgatgat gaccgaacgc aaagccgtcg agcagcaaat cgcccgtctc gcccaaaaag
    16681 cgcgcgccgc cggtatccat atgattgtcg ccacccaacg tcccagtgtc gatgtcgtta
    16741 ccggcctgat taaagccaac atcccgacgc gtatggcgtt taccgtgcaa agcaaaatcg
    16801 acagccgtac catcctcgac caaatgggcg cggacgaact gctcaaatat ggcgattcgc
    16861 tgttccttca gcccggcagt gccgaaccga cccgcctgca aggcgcgttt gtttccgatg
    16921 atgaagtcca ccacgtcgtc gctttcgtca aggagcaggc accggcaaat tatgtagaag
    16981 ggctgcttac aggcgaagcg gcacaggaaa cggcaaatat cgtcagcccc aatgcagaca
    17041 gtgacgaatt gttcgatcag gctgtcgcct atgttttgga aagcaaaaaa acctccattt
    17101 cgtctttgca gcggcagttg cgtatcggct ataaccgcgc ggcaaacctg atggaggcac
    17161 tggaaaatgc aggtgtcgtt tctccctccg atcttaacgg cagccgtaaa attttggcgc
    17221 acaaggacca tttatagccc atatcgcaaa atgccgtctg aacggtggaa ttggcgtttc
    17281 agacggcata ttatgtttca ggcgaaacat tgtgatatac ttgccagcta aaatttcccc
    17341 tttgcggcaa tgcggttcaa atatcgtacc gttgcgctgt ttgcttcccc atgtagggaa
    17401 gaaagtttat cattttatca acacaacaaa tttaagggct tatgatgagc gtaactgttg
    17461 aaactttaga aaatctggaa cgcaaagtag tgttgtccct gccttggtcc gaaatcaacg
    17521 cagaaaccga taaaaaactg aaacaaaccc aacgccgtgc aaaaatcgac ggtttccgtc
    17581 cgggtaaagc acctttaaaa atgattgccc aaatgtacgg tgcgagcgca caaaacgacg
    17641 tgatcaacga gctggtgcaa cgccgcttcc acgatgttgc cgttgcccaa gagttgaaag
    17701 tggcaggctt cccccgtttt gaaggcgttg aagaacaaga cgataaagaa tctttcaaag
    17761 ttgccgccat ttttgaagtg ttccccgaag tcgttatcgg cgatttgtct gcacaagaag
    17821 ttgaaaaagt aaccgcttcc gtcggtgatg ccgaagtgga ccaaaccgta gaaatcctgc
    17881 gcaaacaacg cacccgcttc aaccatgtcg aacgcgaagc ccgaaacggc gaccgcgtca
    17941 tcattgactt tgaaggcaaa atcgacggcg aaccttttgc cggcggcgca tccaaaaact
    18001 acgccttcgt attgggcgca agtcaaatgc tgcctgaatt tgaagccggc gtagtcggca
    18061 tgaaggcggg cgaaagtaaa gacgttaccg tcaatttccc tgaagactac cacggtaaag
    18121 acgttgccgg taaaactgcc gtgttcacca ttacgctgaa caacgtttcc gaagcgactc
    18181 tgcctgaagt cgatgcagat tttgcaaaag ccttgggtat tgcggatggc gacgttgcca
    18241 aaatgcgcga agaagtgaag aaaaacgtaa gccgcgaagt ggaacgccgc gtaaacgaac
    18301 aaaccaaaga atccgtaatg aacgcgctgc tcaaagccgt agagctgaaa gcacctgttg
    18361 ctttggtcaa tgaagaagcc gcacgcttgg caaacgaaat gaaacaaaat tttgttaacc
    18421 aaggtatggc tgatgctgcc aacttggatc tgcctttgga tatgttcaaa gaacaagccg
    18481 aacgccgcgt gtctttgggt ctgattttgg ccaaactggt tgatgaaaac aaactggaac
    18541 cgactgagga gcaaatcaaa gccgttgtcg ccaacttcgc agaaagctac gaagatcctc
    18601 aagaagtaat cgactggtac tacgcagagc cttcccgcct gcaagccccg acttctttgg
    18661 cgatagaaag caacgtcgtt gatttcgttt tgggtaaagc caaagtaaac gaaaaagctt
    18721 tgtcttttga cgaagtgatg ggcgcgcaag cctgattatc ctgaaaatgc cgtcttaggg
    18781 cagtttttga aagcaccgaa gccgctttta aagcccgctt tggtgctttt acatcatgaa
    18841 aggagacgaa atgtcttttg ataactatct tgtccctacc gttatcgagc agagcggtcg
    18901 cggtgagcgt gcattcgata tctattcccg gcttttgaaa gagcgcatcg tattcttggt
    18961 cggcccggta accgacgagt ccgccaatct ggtggttgcc caactgttgt ttttggaaag
    19021 tgagaatccg gataaggata ttttcttcta tattaactcg ccgggcggtt cggtaacggc
    19081 cggtatgtcg atttacgaca ccatgaattt tatcaagccc gatgtatcga ctttgtgctt
    19141 ggggcaggca gcaagtatgg gcgcgttctt attgtcggca ggcgaaaaag gcaaacgttt
    19201 cgccctgccc aacagccgga ttatgattca ccagccttta atcagcggcg gcttgggcgg
    19261 tcaggcatcc gatattgaaa ttcacgcacg cgaactgttg aaaatcaaag aaaaactcaa
    19321 ccgcctgatg gcgaaacatt gcggccgcga tttggcagat ttggagcgcg acaccgaccg
    19381 tgataatttc atgtctgccg aagaagcaaa agaatatggt ttgatcgacc aagttttgga
    19441 aaaccgcgct tctttgcagc tttaaacctt gtgaagtaga caaatgccgt ctgaaaacag
    19501 ttcagacggc atttgtctta tagggactgt attgggatga ttttgttctg gttgcgtttg
    19561 gagccgtttt cgccgtagtt ggttttgaca tactcttcaa caatagccaa aaattccgtt
    19621 gcaatgttgt cgcctttcag cgttactttg cgttcaccat ctacataaac aggcgcgacg
    19681 ggtgtttccc ccgtaccggg caggctgatg ccgatgtcgg ccaatttgct ttctccgggc
    19741 ccattaacaa cgcagcccat tacggcaacg ttcagggatt caaccccagg ataaagggta
    19801 cgccatatag acattttttg gcgcaggtaa ttttgaacat cttgtgccag ctcttgaaat
    19861 acggtactgg tggtacgccc gcagccgggg caggcggtaa ccatcggcgt aaacgaacgc
    19921 aatcccatag tttgtaaaat ctcttgcccg acgacgacct cctgagtacg cgggctgcca
    19981 ggttccggag tcagtgaaat gcggatggtg tcgccgattc cttcttgaag caagacggat
    20041 aatgccgccg ttgatgcgac aatgcctttg ctgcccatac cggcttcggt caaacccaaa
    20101 tgcagcggat aggcgcaacg gctgcccagt tcgcggtaaa cctgaatcaa atcctgaacc
    20161 gcgctgactt tgcacgacag gatgattttg tcttcgggca gtcccaatag aacggctttt
    20221 tcggcagatt ccaaagcgga gacaatcagt gcttccttcg tcacttcttc gggcggtttc
    20281 ggcgcggaag aagcgaggtt ggcatccatc atacgtttgg cgaggctttg atccaaagaa
    20341 ccccagttta cgccgatgcg gacggcttta tcgttttcag cagcagtccg aatcataaag
    20401 gcaaattttt catcgccttt tacgcctttg ccgacattgc cgggattgat gcggtatttg
    20461 gacaatgctt tgccgcattc tggaaattcc gccaacaggc gttcgccgtt gaagtggaaa
    20521 tcgccgataa gcggtgtggc atagcccata tcgtccaagc ggcggcggat ttcggcaact
    20581 ttggacgcgg cttcggggct gttgacggta atacgcacca tttcggatcc ggcgtcgctc
    20641 aattccttaa tctgcaatgc ggtggctttt gcatcggcag tgtcggtgtt ggtcatagat
    20701 tggataacga cgggtgcttc tgaaccgacg gtaatatgat cgatgcggac ttgatgcgtc
    20761 ttgcggcgtt ggagtgtgtt catatggttt gaatctgttt attgaccgat gaggacggtt
    20821 tgcaattctt ccgagtaggg gaaattcgcc tgcaattgtg cttcatattc gtatgccgcc
    20881 tgtgcgttgc cgagggcttt ggcaattttc cagcctagca gcaaatcatc ggcctgaagg
    20941 acttctaccc tgctttggta ttttttaaag tagtaatcgg catcgcccaa ctgcccggcc
    21001 agcattttgg tgcgcgccag ttctttaaat gcgggtggga actgcggctg ggcggcgagg
    21061 gaacgtttca aataggcttc cgccaatccg aattgcccct gttttgcgct gcatatgcct
    21121 ttattcaggt tggcaatata aggggtcggg taggtggggt cggccagggc tttgtcgaaa
    21181 tatgccatag attcggcagg gcggttgagc ctgccgcaca ggaaccagcc gtagttgttg
    21241 ttgatttcgg cactgtcggg tttgatggag agggcttgcc ggaaactttc ctgcgccttg
    21301 tcgttaactt tcaggtattg atagatttcg gcacggacca gccaggcaag ctcgttttta
    21361 gggtctgatt tcaaggcgtc ttcaatactt gccgtcgcct gacggtagtc ctgaccgcgc
    21421 atatattcca ttgccaactg ggttttgata ttggaaacct gattggcttt ttctgcccgc
    21481 gaggggcggt aggaagtgct gcacgcgccc aaggcaagga caagtaataa agagattcgt
    21541 ttggatggct taaaaggcat aattacccct gttgtccgat taaaatctgc tgccattttt
    21601 gttggcggcg cgttttatcc tgaacctgcc ccgccaactg tccgcaggcg gcatcgatgt
    21661 cgtcgccgcg cgtttttcgt acggtaacga caaatcctgc ctgctgcaaa atatcgcgga
    21721 acacacggat gttctcattg ctggagcgtt cgtatccgga gtttgggaag ggattgaacg
    21781 gaatcagatt gaacttgcag ggaacatctg tgaccagttc gatcagttcg cgcgcatgtt
    21841 gcgccttatc gtttattccg tccaacatga cgtattcgaa agtgatgaaa tccctgggtg
    21901 ctttgaccag atagcgttgg catgcggcca tcaattcttt caagggatat tttttgttca
    21961 acggtacgat ttggttgcgg acttcgtcat tggaagcgtg gagggaaacc gccaaagcca
    22021 ccggcatgac atcgcgcaac ctgtccattt ggggaaccat acccgaagtg gaaacggtta
    22081 cgcggcggcg gctcaaaccg tagccgtggt cgtccagcat gatgcttaag gcggtaacga
    22141 cattgtcgaa gttcgccatc ggctcgccca tacccatcat cactacattg gaaattacgc
    22201 gttcgttttt cggtgtaaca cccatcgctt tgtttgccca ccacaattgc ccgatgattt
    22261 cggcagcagt caaattgcgg ttgaagccct gccggccggt cgaacaaaat gtacattcca
    22321 aagcgcagcc gacttgtgag gaaatgcaga gcgtgccgcg atccgattcg gggatgaaga
    22381 cggtttccac cccgttgccc gtaccgacat ccaaaagcca ttttcgagtg ccgtctgaag
    22441 atttttgaga catcatcagc ttgggaattt cgatgcctgc ctgttcgttc agtttatggc
    22501 gcaacgattt tgccaaatcg gtcatttcgt taaaattttg cgcgccggat tggtgcatcc
    22561 aacgcataac ctgtttggca cggaagggtt tttcgcccat atcggcaaaa tgtcgggtca
    22621 gcccttgaag gtcgtagttg agcagattgg ttttcatgtg tttgtgtttt tcttaaatca
    22681 aggcttcaaa taaaaaattg cagggcaggc aagtttgatt tgaagccttt tgcaacagag
    22741 tcttcagacg gcatggatat attttatgcc gtctgaagag gagttgggcg gtgtattgta
    22801 tcaacggggg cagatttcgg tttggctgaa aaagtaggca atttccaaag cggcattttc
    22861 cacgctgtcg gaaccgtgta cggcattaat gctgaccgaa gtggcaaagt ccgcgcgtat
    22921 cgtgccttcg gcggcttcgg aaggattagt tgcacccatc agttcgcggt ttttcaggac
    22981 ggcgttttca ccctctaata cctgaatcat aaccggaccg ccggtcataa attcaaccaa
    23041 tccggcgtag aaggggcggt ctttatgaac cgcataaaat tcttgcgcct ctttgagggt
    23101 aagctgcttc attttggcgg caacgatttt cagaccgttc tcctcaaagc ggctgtatat
    23161 tttgccgata acgtttttgc cgacggcatc gggtttgatg atggagatgg tacgttcaat
    23221 cgccatgcta tatccttatt tttacttaag aagaatcaaa tcggtattct atcaaaaaaa
    23281 taacttatcc gcctgaagcc ttgggagata gggtcaggtg cggctgttcc aaataaactt
    23341 cggactgcat ttcgaccatc cggctggcgg tgcgggtaaa ttcttcggca aaatcgcctt
    23401 ccgtatagat atggttgaca tctaccgcgc cggtggcaca cagtttgacc cggaaatcgt
    23461 agagtacgtc aatcagccaa gtcagccgcc gcgcctccgc cttttcttgc ggtgagagtt
    23521 gttccaaacc tgaaataaaa accatttcat aatgttcggc caaatacaga tagtcggact
    23581 gtgagcgggg gccgaagcac agtgcgcgga aatcaaacca tatggcacgg ccggactcgg
    23641 ctttgtgggg aatctcccga ccatggatgg tgctgatgcc ggggttcaaa tcggtaatgc
    23701 ctgtcatttc tttgaacagt tttgccagtt ttgcctcatt ttcttcattg gcaggcgtaa
    23761 agaaaatctc ggcggggcgg agggtacgca gtcggtagtc ttcaccgccg tcaacgttta
    23821 agacggtcag gctggactcg atgagcgcga ttgtgggaag aaaactgctc cggttttgac
    23881 cttgcgggta gagttcggaa ggcgcgtagt ttgaagtcgc caccaaaaca acgccctcgt
    23941 taagcaggtt ttccagcaga cggcctaaaa tcattgcatc cgcaatatcg ctgacatgaa
    24001 attcgtcaaa acacaatacg cgggtttctt tggcaatctc ggcggcaacg gatttcaacg
    24061 ggttgctttc gcttttcagg gttttcagcc tctggtggat ttctgccata aaggcatgaa
    24121 agtggacgcg gcgtttgcgg cggtaaggga ggcagccgaa aaaagcgtcc atcagaaagc
    24181 ttttgccgcg tccgactccg ccatagaaat aaagcccttt ggggacttgc ggggaacgca
    24241 aactcctgcc taaaaaacgg tttcttttgc gtttgaacat catcaattcg gtccaaagcc
    24301 gatcgaggtg ttcgatggcg gctgcctgcg cgtcgtcgcg gatgaagttg ggcagttgtg
    24361 aggcagcctg ataccaagtc agcgggctgt gattttcaaa cggcggggct ttaaaaagtt
    24421 ggtctctatt actcatattt aaccttgtta tacttcttgc ggcggagaaa gaaaatgctt
    24481 accgtcggta ttataaatga tttccttcaa acttggcgtt ttccgtggaa aagaataatg
    24541 acgcgcacaa gggggggtat gagcaaaggc ggcgtttcaa tatggtgcag atatgagctc
    24601 aaatgccttt tatagt
//
LOCUS       DAWKQB010000013        24090 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.013, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000013 DAWKQB010000000
VERSION     DAWKQB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24090
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.013"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(131..2032)
                     /gene="thiC"
                     /locus_tag="WJL14_000407"
     CDS             complement(131..2032)
                     /gene="thiC"
                     /locus_tag="WJL14_000407"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ7655534.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGSNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTRPDQITPEFVRQEIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
     regulatory      complement(2123..2222)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2372..2448
                     /locus_tag="WJL14_000408"
     tRNA            2372..2448
                     /locus_tag="WJL14_000408"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2406..2408,aa:Met,seq:cat)
     gene            2630..3505
                     /locus_tag="WJL14_000409"
     CDS             2630..3505
                     /locus_tag="WJL14_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7655535.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            3514..4452
                     /locus_tag="WJL14_000410"
     CDS             3514..4452
                     /locus_tag="WJL14_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7655536.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYTLFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEVHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRGTLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(4895..6670)
                     /gene="ptsP"
                     /locus_tag="WJL14_000411"
     CDS             complement(4895..6670)
                     /gene="ptsP"
                     /locus_tag="WJL14_000411"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ7655537.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAAH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLESDIVKVIRCEDE
                     EKSEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(6670..6939)
                     /locus_tag="WJL14_000412"
     CDS             complement(6670..6939)
                     /locus_tag="WJL14_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ7655538.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(7008..7445)
                     /locus_tag="WJL14_000413"
     CDS             complement(7008..7445)
                     /locus_tag="WJL14_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ7655539.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(7526..8089)
                     /locus_tag="WJL14_000414"
     CDS             complement(7526..8089)
                     /locus_tag="WJL14_000414"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ7655540.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(8158..8982)
                     /locus_tag="WJL14_000415"
     CDS             complement(8158..8982)
                     /locus_tag="WJL14_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ7655541.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            9073..9705
                     /locus_tag="WJL14_000416"
     CDS             9073..9705
                     /locus_tag="WJL14_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ7655542.1"
                     /translation="MTLRYEILSVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(9911..11695)
                     /gene="rmuC"
                     /locus_tag="WJL14_000417"
     CDS             complement(9911..11695)
                     /gene="rmuC"
                     /locus_tag="WJL14_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ7655543.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     QGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQNSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTERANETTALSESLEYVAEDEAV"
     gene            complement(12034..12822)
                     /locus_tag="WJL14_000418"
     CDS             complement(12034..12822)
                     /locus_tag="WJL14_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ7655544.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(12837..14186)
                     /locus_tag="WJL14_000419"
     CDS             complement(12837..14186)
                     /locus_tag="WJL14_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ7655545.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDVLGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(14205..14786)
                     /gene="petA"
                     /locus_tag="WJL14_000420"
     CDS             complement(14205..14786)
                     /gene="petA"
                     /locus_tag="WJL14_000420"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ7655546.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(14909..15658)
                     /locus_tag="WJL14_000421"
     CDS             complement(14909..15658)
                     /locus_tag="WJL14_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ7655547.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            15789..16718
                     /locus_tag="WJL14_000422"
     CDS             15789..16718
                     /locus_tag="WJL14_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7655548.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAERDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITDDGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(16973..17365)
                     /gene="rpsI"
                     /locus_tag="WJL14_000423"
     CDS             complement(16973..17365)
                     /gene="rpsI"
                     /locus_tag="WJL14_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ7655549.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(17375..17806)
                     /gene="rplM"
                     /locus_tag="WJL14_000424"
     CDS             complement(17375..17806)
                     /gene="rplM"
                     /locus_tag="WJL14_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ7655550.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(17984..18163)
                     /gene="ssrS"
                     /locus_tag="WJL14_000425"
     ncRNA           complement(17984..18163)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WJL14_000425"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(18169..18474)
                     /locus_tag="WJL14_000426"
     CDS             complement(18169..18474)
                     /locus_tag="WJL14_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ7655551.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(18485..18814)
                     /locus_tag="WJL14_000427"
     CDS             complement(18485..18814)
                     /locus_tag="WJL14_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655552.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QRQETQQ"
     gene            complement(18869..19858)
                     /locus_tag="WJL14_000428"
     CDS             complement(18869..19858)
                     /locus_tag="WJL14_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ7655553.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWVE
                     ELVPQLNTSVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(19981..22683)
                     /gene="ppc"
                     /locus_tag="WJL14_000429"
     CDS             complement(19981..22683)
                     /gene="ppc"
                     /locus_tag="WJL14_000429"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ7655554.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRSELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADIIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSEHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQDLSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGNPDTLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            22726..23619
                     /locus_tag="WJL14_000430"
     CDS             22726..23619
                     /locus_tag="WJL14_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ7655555.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TKHDNDDELLLRYSRHILLDEIGIEGQQKLSGAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADFDTVELHNLQRQVAFNEGDVGKPKAEALAGRLKRINHTVNVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
BASE COUNT         5168 a         6514 c         6454 g         5954 t
ORIGIN      
        1 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
       61 tttcacaagc gaaagaatac cgtctgaagc cttttttccg ggtttcagac ggcatttttt
      121 gcttgacgtt tcaactgtaa atcttcgcgc cttttttgac gaactcgacc gctttttcct
      181 ccatgccctg ccgttgggct ttttgcttgt cggcgtagtc gcgcacttcc tgcgtgattt
      241 tcatcgagca gaatttgggg ccgcacattg agcagaagtg ggcgattttc gcgccttcgg
      301 caggcagggt ttcgtcgtgg aagctctcgg cacgttcagg gtcgaggctt aagcgaaatt
      361 ggtcgcgcca acggaactcg aaacgcgcct tgctcagggc gttgtcacgt aattgtgcgc
      421 ccggccagcc tttggcgaga tcggcggcgt gggcggcgag tttgtaggtg atgatgccgg
      481 tgcgcacgtc ttctttgtcg ggcagcccca aatgttcttt gggggtaacg taacaaagca
      541 tcgccgtgcc gtaccagccg atattggccg cgcctatgcc cgaggtgatg tggtcgtagc
      601 cgggtgcgat gtcggtaacg agcgggccga gtgtgtaaaa aggtgcttca aagcagtgtt
      661 gcagctcttc ggtcatgttt tctttgacgc gttgcagcgg cacatggccg gggccttcga
      721 tcatgacttg tacgtcatgt ttccacgctt tatcggtcaa ttcgcccaag gtgtgcagtt
      781 cggcgaattg ggattcgtcg ttggcatcgg caatgcagcc ggggcgcagg ccgtcgccga
      841 ggctgaacga tacgtcatac gctttcataa tttcgcagat ttcgtcgaaa tgcgtgtaga
      901 ggaaattttc ccgatgatgt gccaaacacc atttcgccat aatcgaaccg ccgcgcgaga
      961 cgatgccggt gaggcggttg gcggtcatcg gtacataacg cagcaacacg cccgcgtgta
     1021 tggtgaaata gtccacgcct tgctccgcct gttcgattaa ggtgtcgcgg aacaaatccc
     1081 aagtcaaatc ttcggcaatg ccgccggttt tttccaaagc ctgataaata ggcacggtgc
     1141 cgatggggac gggcgcgttg cggataatcc attcgcgcgt ttcatggatg tgcgcgcctg
     1201 tggataagtc cataattgtg tccgcgcccc aacgcagcga ccacaccatt ttttcgactt
     1261 cttcggtcag gctggaagtg acggcggaat tgcccaagtt gccgttgatt ttgacgcgga
     1321 agttgcggcc gataatcatc ggttcgagtt cgggatggtt gatgttggcg gggataatcg
     1381 cgcgtccggc ggcgatttct tggcgcacga attcgggcgt gatttggtcg ggacgggttg
     1441 ggatgttcgc gccgaaactt tgccctgcgt gttgtttcaa gagtttggcg tattccgatt
     1501 tttgggacaa ttcgtctaat tttaaacgtt cgcgtatggc gacaaactcc atttcgggcg
     1561 tgataatgcc ttggcgcgcg tagtgaagct gggttacgtt gctaccgctt ttcgcgcggc
     1621 gcgggcgggt gatttggttg aagcgcagat gggcggtttt cggatcgtgt gcgcgttcga
     1681 tgccgtattc gctggagagc ttgggcagga tttcggtatc gccgcgttcg ttcagccatg
     1741 cggtgcggat gtgcggcaga ccttgtttca ggtcgatgtg tgccgctgga tcgccgtaca
     1801 cgccgctggt gtcatagacg ggaatcggcg ggttggcttc cgcgccttgc gccgtatagg
     1861 tgtcgtcctg acggatttca cgcaaaggca cgcggatgtc gtcgcggctg ccttgcagat
     1921 acacgcgttc cgagttcgga tatttgaagc ggataccgat gtcttcgctc aagtcggcaa
     1981 gttcgcgcgc ttcgttgccg gaagttttgg cggttttttt tggcgtagtc ataaaaaatg
     2041 ctcctgtttt ctcgtttaga ataaagaaac aggagcgttt tgcgttttca gacggcattt
     2101 gaaaaccaat gccgtctgaa aagcagaatc cgtgaaaact ccccacgcag gtattatcca
     2161 gatcgggtgt aaagggtatt tctcagccgc ctgaacatca tgcagcaccc ctgtttcaat
     2221 gttaaccaaa attaaatcac gaacatgaac ttttgtaaag aaaataatat ttcaaatcag
     2281 gcataaacca ccggacggca aaattttatg atttttcgcg gaaataatgt ttgacaacat
     2341 aaaaaatctg ccgtatagtt tcatcttctg acgcgggatg gagcagcatg gtagctcgtc
     2401 gggctcataa cccgaaggtc gtaggttcga atcctgctcc cgcaaccaaa tatcaaaccc
     2461 ctcggttcaa tgccgagggg ttttgttttg cctgtttcct gccgcctccg ttttttgccg
     2521 gattttcctt cctgccgcaa tatcggaacg gcagcccgcc gtctgtttgc ggttgcaaat
     2581 tcaggcagtt tggctacaat cttccgcatt gtcttcaaga aagccgacta tgccgaccgt
     2641 ccgttttacc gaatccgtca gcaaacacga ccttgatgcc ctgttcgagt gggcaaaagc
     2701 aagttacggt gcagaaagtt gctggaaaac gctgtatctg aaccgtctgc ctttgggcaa
     2761 tctgtcgccg gaatgggcgg agcgcgtcaa aaaagactgg gaggcaggct gctcggagtc
     2821 ttcagacggc attttcctga atgcggacgg ctggtcagat atgggcggcc gcttgcagca
     2881 cctcgcccgc acatggaaca aggcggggct gcttcacgga tggcgcaacg agtgtttcga
     2941 cctgaccgac ggcggcggca accccttgtt cacgctcgaa cgcgccgctt tccgtccgtt
     3001 cggactgctc agccgcgccg tccatctcaa cggactgacc gaatcggacg gccgatggca
     3061 tttctggata ggcaagcgca gtccgcacaa agcagtcgat cccgacaaac tcgacaatat
     3121 tgccgccggc ggtgtttccg gcggcgaaat gccgtctgaa gccgtgtgcc gcgaaagcag
     3181 cgaagaagcc ggtttggata aaacgctgtt tccgtttatc cgtccggtat ctcggctgca
     3241 cagcctgcgc cccgtcagcc ggggtgtgca caatgaaatc ctgtatgtat tcgatgccgt
     3301 cctacccgaa accttcctgc ctgaaaatca ggacggcgag gttgcgtgtt ttgaaaaaat
     3361 ggacatcggc agcttggttg ccaccatgtt gtcgggaaac atgatgcacg acgcgcaact
     3421 ggttacgctg gacgcgtttt gccgttacgg tctgattgat gccgcccatc cgctgtccga
     3481 gtggctggac ggcatacgtt tataggatgc gccatgcttg aactgaacgg actctgcaaa
     3541 cgcttcggcg gcaaaacggt tgccgacaac atctgcctga ctgtcgggcg cggcaaaata
     3601 ctcgccgtac tggggcggtc gggctgcgga aaatccaccc tgctgaatat gattgcgggc
     3661 atcgtccggc cggacggcgg cgaaattcag ctgaacgggg aaaacattac ccgtatgccg
     3721 cccgaaaaac gccgtatttc gctgatgttt caagattaca cgctgtttcc ccatatgagt
     3781 gcgctggaaa atgcggcatt cggtttgaaa atgcaaaaaa tgccgaaagc cgaagccgaa
     3841 cgccttgcca tggcggcact tgccgaagtc ggactggaaa acgaggtgca ccgcaagcct
     3901 gaaaaacttt ccggaggcga gaaacaacgg ttggcactgg cgcgcgcttt ggttgtccgc
     3961 ccttccctgc tgctgttgga cgaatcgttt tccagtttgg acacgcattt gcgcggcacg
     4021 ctgcgccgca tgactgccga acgcatccgc aagggcggca tccctgccgt tttggtaacg
     4081 cattcgcccg aagaggcctg cacggcggcg gacgaaatcg ccgtcatgca cgaggggaaa
     4141 atccttcaat gcggtacgcc cgaaaccttg gttcaaacgc ctgccggcgt gcaggtcgcc
     4201 cgactgatgg gtttgcccaa taccgacgat gaccgccata ttccgcaaca tgcggtgcgt
     4261 ttcgaccaag acggcatgga gtgccgcgta ttatcccgta cctgtttgcc cgaatcgttc
     4321 agcctgtccg tcctccatcc ggaacacggc atcctgtggc tgaacctcga tatgccgcac
     4381 gccggtgaaa tatcgggaaa cgatacggta cgcatccata tcgaagacag ggaaatcgtc
     4441 cgcttccgct gatgcttctt aaaaacaaaa tgccgtctga aaacctttca gacggcattt
     4501 tttaccaaag cagccatatt ttttatcagg gctgcaaaat ttatccgaaa caacagaaac
     4561 caccctgccg tcattcccgc aaaagcggga atctagaacc caacacggca aaaatttatc
     4621 cgaagcgaca acaatctttt catcgtcatt cccgcgcagg cgggaatcta gaacgtaaaa
     4681 tctcaagaaa ccgttttacc cgataagttt ccgcaccgac agacctagat tcccgcctgc
     4741 gcgggaatga cggattttag gtttctgatt ttggttttct gtttttgagg gaatggccga
     4801 ttttgggttt ctgtttcggt tttctatttt gcaagaatgg caaaatttca gattgcgggc
     4861 attgttaagt atttctattt tttacctgcc gtatttattt ccgccccttg aagtcggctt
     4921 cttcctcgac agacacgctg ttcatctgtt tgatcagctt ttccgacttc tcttcgtctt
     4981 cgcagcggat gactttcaca atatcgcttt cgagctgtcc gacattgctg tgcagaatga
     5041 tgtttttgac gggcaggatg ttgttggggt tcatggaaaa acggcgcagc cccataccca
     5101 ataaaacgcg ggtaaacgcg gtatcgcccg ccatctcgcc gcatacggat acgtctttgt
     5161 ccatgcggtt ggcggtacgg atgacgtgtt gcagcatttt cagcacggcg ggatggccgg
     5221 gctggtagag gtggctgacg ctgtcgtcgc cgcgatcgac ggacaagatg tattgaatca
     5281 ggtcgttggt accgacggag atgaaatcga ccagtttcaa aatactgccg acggtcagcg
     5341 cggcagacgg aatttcaatc atacagccga tgccgacttt accgaaggca tcgccgcgtt
     5401 cggcaagctg gcgttgcgcg gtgtcgaggt ggatgaggca ctggcgcact tcggatacgg
     5461 aggtaatcat cggccacatc atccgcacgg ggccgtgggc ggcggcgcgg aggatggcgc
     5521 gcatctgggt gcggaacatg accggttcgg caaggcacag gcggatgccg gtcatgccca
     5581 gcgcggggtt gaggctgccg ttgggcgtgc tgtttttccc gaaccagcgc gggtttttgt
     5641 ccacacccaa atcgactgtc cgtatcgtta cgcttttgcc tttcattttt ttgacaatcg
     5701 cgctgtacac ttcgtactgc tcgtcttcag acggcatcgt atcgcggttc aggtaaagaa
     5761 actcgctgcg gaacagcccg atgccgtctg cgccgaggtt gtgcagcggt ttcacgtctt
     5821 cggcggattc tatattgccc acaagctcga tgcagacccc gtcagcggtg gcggcggcgg
     5881 tttttttgag cttgttcaaa tcgcgtttgt ggctgcggta ttcgcgggca cggcggcggt
     5941 attcgttcaa caccgactca tccggcgcga taatcaacac gccgttgata ccgtccacaa
     6001 tgaccgtttc gccctcggta atcagtttgc gcgcgttgtg cagcccgacg acggacggga
     6061 tgtccaagct cctgcccaaa atcgccgtat gcccggtggg gccgccggca tcggtaacga
     6121 aggcggcaat gcgctgctct ttaaacaaaa ccgtgtcggc gggcgaaagg tcgtttgcaa
     6181 tcagaacggt ttcgtcaaac aggttgtcgg caacttccaa ctcgttgccc tgcccgatca
     6241 ggttgttgtg gatgcggcgg acgacttgca gcatatcctg cttgcgttcg cgcaaatagg
     6301 catcgtccat attgtcgaat tgggcggcga gtttgtcgct ctgctgcttc aatgcccact
     6361 cggcgttgat tttttgttcc cttaaaatat cgacgggttc gcgcgacaag gtaacatcgg
     6421 tcaagagcat caggtgcagc gagatgaacg cgcccaactc ggtcggggcg ttttcgggaa
     6481 tcgcgctgcg gagctgttcc aactctttgc gcgtggcttt gacggcggca tcgaaacgtt
     6541 cggcttcggc atcggtgtcc gcctccgcaa catcatactg cggcacttcc tccgtaccgc
     6601 gcgcaatcag gtgggcgcaa ccgacggcaa tgcctttgcc cgccgccacg ccgtgcagca
     6661 cgatactcat tattcgccct cgccgaagta gccgttgatt aagtcggtca gggcgcgcat
     6721 cgcttccgcc tcgtccgcgc cgtccgtctc cagttcgatg accgtaccct tggcggcggc
     6781 gagcatcatc agccccataa tgcttttgcc gttgacgcgg ctgtcgtttt tcgtaaccca
     6841 gacttcgctt ttgaattggg acgcggtttg ggtgaacttg ttggacgcgc gggcgtggag
     6901 tccgagtttg ttgatgattt cgatggattg tttgagcatt tcgattcccg tgttatgtat
     6961 atcggcagca gacgccgttt aaaatgtttt cctgccctgc cgcttcttca gacggcatcg
     7021 ccgctgcgcc ggcacaccaa atcttcgggc gcggacgtga tggcgaaaat gccttttacc
     7081 gccgcctccc tgacgcattc ggtaaaggcg gcaaggtctt ccgccgccgg cgaatattgg
     7141 acggccttaa ccatcatcgg cgcgttcagc ccggtcaaaa tcgccgattt gttttcgcgc
     7201 acgaggcggc gggctgcatt gcagggggtc gcgccgaaaa tatcggtcat aattagcacg
     7261 ccgtcgttgt cgggaaactc ctgaagcgcg gcaatggcgt tgttgatgat gtcgtcttgg
     7321 tcttccgtcg gttgcacgcc gagtatgcgg acgttttcag gcagtccgcc cggaaaaaaa
     7381 tgatgtgcca gcttgcggta ggcttcgcct atggtttcgt gtgtgatgat taaaagccct
     7441 atcatattat gcgtcctgtt cctcgttgtc ctaccggcgt atgggcgcga tgccgtctga
     7501 aaggccttca gacggcatcg cgtccttatt ttccgtccaa tgcgtaaatc tcgcccagat
     7561 tgcgccagca gcccgccgca tccatgccgt aaccgaaaac ataacggttc ggcacatcca
     7621 gtccgacata atcggctcgg ataggcttgg gtttgtcaat cagcttgttg gcgaacaccg
     7681 ccgcacggca gcttgccgca cccatttcca aaagtttggc ttgaatggcg gacatcgtat
     7741 gcccttcgtc caaaatatcg tccagcacga cgacgtgcct gccccggatt tgttccgcat
     7801 cgggcatacg cttccagttg aacgcgccgc cctccagctt gtcgccgtaa cgggaaacgt
     7861 gaacataatc aaaatctaag ggaaaacgca acagcggcag caactgcccc gtaaacacca
     7921 ccgcgccgcc catcacgggc agcagcagcg gatatttgcc gcccaaatcg cgcgtaatct
     7981 cgtccgccac tttttgcagt gcggcacggc attggccttg gtcgaacaaa agatcagcgt
     8041 tttcaagcat cgcctgtgtt tcaaggcgtt tggtttctaa atcggtcata tgtcggaatc
     8101 ggtcggtaaa aggaaaatta taaaccaaag tatcggatgc cgtctgaact gtcctgctca
     8161 gcggtcggta cgcacgcgca caaatgtggc aaatttcggc gtgcctttcc gcgtaaagcc
     8221 acggtaacgg taggtaatca gcgtgccgat tttgggcggg ttgtcgcggt ctttgtcctt
     8281 gaaaccgctg ccgatgcgga attcgccgtg tcggtttttg cagccgaccg cgcccagccg
     8341 tccggcgttt cgccctttgc cctcatagtg ccgcgttacc gtgcattcgt cgtcgtattg
     8401 gcttttcagc ttcaataatt ggctgctcct gccgccgcta taacgggatt cgggctggcg
     8461 cagcatcacg ccttcgccgc cctgcgcttc gatttgtttt aaaaagtcca tcgcgtgctg
     8521 ccggtcgcgc actttgattt gcgggatgat ggtaatcggc gcgttcggat gcgttttcag
     8581 ccactgcgtt gcgactgcca aacgttggta gagattgccc tgcgccttgg gtacgtcgaa
     8641 aacgtgcaga cggatgccgc gccagtctga agaaacagaa cgcacggtag cggaaatctg
     8701 ctcgaactga ccgcgtccgc tatacaattc gccgtccaaa ggataaggcg gaaactgcgc
     8761 ggtaaagcct ttggggggcg taaacgcgta gccctgacgg cttatcaggt gctttccgtc
     8821 ccaataggcg cgcacgccgt cgagtttctc gctcattgcc cagccggcaa tatcctgccc
     8881 tttgtattcc tgcgccagca tcaaatccgc cgcgcctgcc gatgcaggga tgaaaaccgc
     8941 cgtaaaaatc ggtatgatgc cgccgattgt cttcttaatc atctgattcc ccaaatatca
     9001 aaacaggcgg caaaccgcca taaaacaaac ggcaaacccg atgccgtctg aaaaaccgtt
     9061 taggaacacg ccatgaccct acgttacgaa atcctctccg ttaccccctt ccgccaaaac
     9121 tgcaccctga tttgggacga cgaaagcggc gaagccgtcc tgaccgatgt cggcggcgac
     9181 gtgccgttcc tgctgcaagc gttggcaaac cgcaaactta cgctcacggc aatctggctg
     9241 acgcacggcc atctcgatca cgcgggcggc gtggtcgaaa tgttgaaaac gcataaagtc
     9301 cctgtcctcg ggccgcatcc ggacgatgaa ttcctgctcc aatcgctgcc gcaaaccacc
     9361 gcgcaatacg gatttcctgt ctcgcccgcc tttgcgccga accgttggct cgaagaaggc
     9421 gaaacgctca cggtcggacg ctatgccttt caagtgctgc atattccggg ccatacgccg
     9481 ggacatatcg tcttttattg tgccgaggcg gaattgctga ttgcgggcga cgtgctgttt
     9541 tacgaaacca taggcagaac cgattttccg cgcggcaacc acgccgactt aatcaataat
     9601 atccgcaaca aattattcgc cctccccgaa accgtgcaag ttgtcgccgg acacgggcgt
     9661 atgacttcca tcggacacga aaaacggcac aatccgtttt tctaaccgac ttcactacgg
     9721 tcttcagacg gcgccatctg cactgatgcc gtctgaaacg caaaaggctc agacaaccgc
     9781 cgccttgccg gtgtacctcg ccggacaagg ctttggtaag tacttcaaac aaaccccaaa
     9841 tcagaaccgg tacagttacg ccgcgctttc ggcattcccg cccctgctga aacaagcttt
     9901 tccgcacaag tcagactgct tcatcttctg ccacatattc caaagattcc gacaacgccg
     9961 ttgtttcatt ggcacgttcg gtcaaatcgc gtgcaagctg cttgtttgcc ttcacgccca
    10021 acagacgcag tttctcggcg cggccgacca gattcccgcg cccctcggca agttgcttaa
    10081 atgccgtctg aaagctgttt tgcgcctgat cgatgccttt gccgacgctt tcgagcgtct
    10141 gtacaaagcc gacaaatttg tcgtacagct tgccgccttc gtccgcaatc gccagcgcgt
    10201 tttggttttg ctgctcgttg cgccaaatat tcgccaccgt ccgcaaagtc gccagcagcg
    10261 tactggggcc gaccagcata atccgtttgt cgaaacactc ttggaacaag ccagcgtcat
    10321 tctgcaacgc caacaggtag gccggttcga cagggataaa cataaagacg aaatccaatg
    10381 tgttcacacc ttccaaatcg gtgtaatcct tcagcgacaa gcctttcata tgcgcgcgta
    10441 tgctggcgac atgcgccgcc aactcgcgtg ccgccgtatc cgcatccgcc gcctgcgtgt
    10501 agcgcacata agctgtcagc gagaccttgg agtcaatcac aatctgcttg ttgtcgggca
    10561 ggttgaccaa aacgtcgggc tggaggcggc gcgtgccgcc gtcttcctct tttcggacgg
    10621 atgctgcctg aaccacatat tcccgccctt tctgaaggcc ggaattttcc aaaaccgttt
    10681 ccagaatcat ctcgccccaa ttgccctgaa ccttattctg cgtaccggtc agcgcgttgg
    10741 tcagggcctt tgcctcgctg tgcagctgcg cattcaatcc ttgaagccgt ttcaattcgt
    10801 tttccaacgt cagccgctcg cgcgattctt tatcataggt ttgcttgacc aactcgccga
    10861 aaccttggat gcgttcgttt agcgggttca aaacctgatg gagctgctcc cggttctgct
    10921 cggtaaaacg gcggcttttt tcttccaaaa tcgtgttggc aagattttga aactgatcgc
    10981 tcaaactttt gcgcgcctcg cccagcaagg acagcttctc ttcagaagca aggcgttcct
    11041 gttcgatttg cgttgccaaa cgttcgtttt caaccgccaa accctgtgcc ttttcctgca
    11101 actcggtatg cgactgcctc aaccgctccg cttccgcctc tttttcctgc aaatgggcaa
    11161 tctgtttttc ggctgcggca aaacggttgc cgacatcggc aaggtcgttt tgcacgtcgc
    11221 ggacagtttg gcggctttct tccaaatcgg tttcgattct ttggcggatt tggcgttcca
    11281 aggcatattg gtcggcaaac gccttccgtt cctgtccgag ctgcgttgcc aaacgttcgt
    11341 tttcaaccgc caaaccctgt gccttttcct gcaactcgat atacgactgc ttcagccgaa
    11401 ccgattccgc ctctttttcc tgcaaatggg caatctgctt ttcggctgcg gaaaaacggt
    11461 tgcccaaagc ataattttcg tcctgcaaat gtcggtattt cccgtccaaa acggccaatt
    11521 ccgatgcaat ttctgcgtgt gcctgttcga caaaatcaca tcttgccgcc ttttccgcca
    11581 gttgcgcgtt caaatcggca aactcgccct gaaacctgcc cttcatcagc aaccatgtaa
    11641 acaacacgcc cgacaccaac gccgccaaag gcagcaaaac agtcatcagt tccatcaatc
    11701 atcctaatat tcaaacattt tcacaccgga caaaaccgcc gcttattccg attctacctg
    11761 tttgttcgac atagctccaa aaaatatagc ggattggctt taaacctgtt cgacatcgcc
    11821 ttaccatgct gcttgcggtt tcagaccttt tcctaattca atatcaatct gccacaaacc
    11881 ctgattaagt tcccgatgtc tgacattttt agaatgatgc cgtctgaaat gttgcagcta
    11941 tgttcagacg gcatacggat tcaggctttt caaacggcag gcaaaatgaa aaaagggcaa
    12001 accctaaagg atttgccctt ttgttccaaa cgcttagtgt acgtctttcc aatattcttt
    12061 tttcaggaaa tacgccaaag gcagcataac cgcaaatagg aaaatcatca cgacatagcc
    12121 tatacgtttg cgttgcagtt gtgcaggttc gcccatgtac acaaggtaat tgaccaaatc
    12181 gcgtacatat gcgtcgtact ctttttggat cactttgccg ttaggcagac ggcggctgtg
    12241 caaaccggta gattcccaat acagcttagg cttcatctcg ccgtgttcgt cttttaccat
    12301 aaccggctga cctttggcat ccaactcaac ggcttgaacg ccttgctgtt cccacaacgg
    12361 gtggggcata ccgactttat cgaatacagt attgttccag ccgctcggac gggtcggatc
    12421 tttatagaag ccgcgcatat aagcgtaaag gtagtctgca cctttggaac gcgcaatcaa
    12481 tgtcaaatcg ggcggagcag caccaaacca ttttgccgca tctttcgggt tcatcgccga
    12541 atgcatgaca tcgccgacat tatcggtggt aaacatcagg tttttcttga tttcttcgtc
    12601 agtcaaaccg atgtctttca aacggttgaa acgcataccg cttgccgagt ggcaggacag
    12661 gcaatagttg gtaaagattt gtgcgccgcg ctgcaggctg acttggtcgc gcaaatcgat
    12721 atcgactttc tcatagtgtg catgaccgcc cgaagcaaat gccgcaccca tgggtactgc
    12781 caacagcaag gcagcaaacc aatttttcat agcttgtttc attttgactg ccctcatcag
    12841 atgttggttg caaacaggta agcacccacg gcggtaatgc cgacgtaaac aaagaacatg
    12901 attttttgtt tggcggtgct catggttacg cgttcgggaa ccggtttgtt tttatccagc
    12961 ttggtataga acggcatacc caggaagaat gcgaaataga cgaaggacag gatgcgcgcg
    13021 accaaagtac gcgtatcggt tgccaccatc gcgcccaaaa tacccaaacc gataaaggaa
    13081 atgatgaaca agaccaatgc ggttttgaaa atcgggccgc ggtaacggac ggatttcacc
    13141 tcgcccctat ccagccaagg cagcagggca atcaacacga ccgccgcgcc cataccgatt
    13201 acaccccaaa cctgcgttcc caagaacgag ggaatcgcac gcaaaatggc gtagaacggt
    13261 gtgaaatacc ataccggcgc aatgtgcgga ggtgttttca gcgcatttgc cgcatcgaag
    13321 ttcggcgctt cgaggaagta gccgccgcct tccggtgcga agaacaatac ggcacagaag
    13381 acaatcagga atacaaccac acccaagacg tctttaacgg tatagtaagg atggaatggg
    13441 ataccgtcgc gcgggatacc gttttcgtct ttgtttttct tgatttcaac gccgtcaggg
    13501 ttgttagaac ccacttcgtg cagggcgatg atgtgcgcca caaccaagcc gagcaatacc
    13561 agaggtaccg cgataacgtg cagggcaaag aagcggttca gggtaacgtc ggaaacgttg
    13621 aagtcgccgc gaatccaagt ggacaaatcc ggaccgataa cagggatggc ggagaacagg
    13681 ttaataatta cctgcgcacc ccaaaaggac atctgacccc aaggcagcag gtagcccata
    13741 aaggcttccg ccatcaatgc caagaaaatc agagaaccga aaatccacac caactcacgg
    13801 ggttttttgt acgaaccgta aatcaaacca cggaacatgt gcagataaac aacaatgaag
    13861 aagaaagatg cgccggtgga atgcatgtag cggataatcc aaccgccgga cacgtcgcgc
    13921 atgatgtact cgactgcagt aaaggcagca ggcagatggt aggcgttcag gttgccgtcg
    13981 ggtttgtagt tcatggtcag gaaaatgccg ctgacgattt gaatcacgag gacaagcagg
    14041 gccaaagagc cgaagtagta ccaaaaatta aagtttttcg gtgcgtagta ttgagccaaa
    14101 tgctcattcc acattttaga cagggggaaa cgagcgtcca tccagcctaa caatgctttt
    14161 gctttgctat tggtttggtt tgccataatt atcgttcctt attcttagtc ttcgcccacc
    14221 aagatagttg tgtcgctcaa gtatttatat ggcgggacaa ccaggttggt cggggcagga
    14281 acacctttat atacgcggcc ggccaagtcg aatttcgaac cgtggcaggg gcagaagaag
    14341 ccgcctttcc agtctgcacc caaatcggca ggggcaatgt cgggacggaa ggtgggcgag
    14401 cagcccaaat gggtgcagat accgatggcg acaaggatgt tcggcttaat cgaacgggtc
    14461 tcgtttttag catactccgg ctgctgttcc gcatcggaat tgggatcggt aagttcgccg
    14521 ttcaggcctt tcaggtcttt aagctgctga tctgtacggt tgagcaccca aatcggtttg
    14581 ccttgccact cggcggtcag caactgaccc gcttcgattt tactgacatc cacctcgacg
    14641 gcagcaccgg cggccttggc tttttccgaa gggaaaaaac tggccacaaa cggcgttgcc
    14701 acacccaatg ctgccactcc gcccgcgccg caggtcgcga gtgtcaggaa acggcggcgg
    14761 ccgttgttga tttcttgatt atccattatt cagtcgtcct aatattttgg gaataccgag
    14821 ccattaaacg ttgcaatttt acccagtttg cagtgatact caaagcatta tttaaaataa
    14881 ggtaaagttt tatgatattt ctcaagactc aagccggatt gttttcgtca aaatggcaca
    14941 cttccaaccc gaaaacctct gccgccgatt ctgccagcgc gcgtacgccg taacgttccg
    15001 tcgcgtgatg ccctgccgaa atgaaagccg tacccgtttc attggcaagg tggtattgag
    15061 cttcagagat ttcccccgtc aaatacagat cgacaccttc gtctattgcc gtctgaaaaa
    15121 acccctgcgc cccgccgcta caccatgcaa cccgtcggat ttcgcgttcg ggtttgccga
    15181 tgacgacagg cttacgttgc aaaactgttt caatatgcgc cgccaatgcg ccgagtgtct
    15241 tggcttgttt caggctgccc gagttgagca ggttttgttc gccgaaccgt ttttctgtcg
    15301 caaaacccaa tctgtcggcg agttgggcat tgttgcccag tgtgggatgt gcatccaggg
    15361 gcagatggta gcctgccata ttgatgtcgt gccgtaacag tgcggcaatc cgttcttttt
    15421 tccaaccagt aacggtcggc aactcgtttt tccagaacat accgtgatgt accaaaagca
    15481 aatctgcctt ctgctccaca gcaaaatcaa tcgctgccct gcttgccgtt accgacgtaa
    15541 cgattttccc gatatattcc ctcccttcaa cctgcaaacc gttaggggcg taatctttaa
    15601 acaacgctgt ctgcaatgtt tcattacacc aagtcagaaa atccctgcgc aataccatct
    15661 tttttcctaa tcgctttaaa caagcgggca ttctaatcgc aaaatgtccg gaattcacat
    15721 ttttccgatt tgcacccgca tatgaattat tttaatatgc gccggttcaa tatgccgtct
    15781 gaagccccat ggattccatt atcgaattgc gccacctcaa aaccctgctg gcacttgaag
    15841 aaaccggcag cgtctccctt gccgccaaac gggttttcct tacccaatcc gccctttccc
    15901 accagatccg tatgctcgaa aaccactacg gcacgccgct gttcgaacgc aaatccacgc
    15961 ccttgcgctt taccccggtg ggcgaaaggc tgctgcgcct cgcccacgaa ctgatacctc
    16021 aagttgctgt tgcagaacga gatttggcgc gaatcacaga aggggaggcg ggagagctgc
    16081 ggattgccgt cgaatgccat acctgtttcg actggctgat gcccgccatg ggcgaattcc
    16141 gcccgatgtg gccccaagtc gaattggata tcgtatcggg attccaagcg gatcccgtcg
    16201 gactgctgct gcaacaccgt gccgaccttg ccattgtttc cgaagcggaa aaacaaaacg
    16261 gtatttcttt ccaaccgctg tttgcctacg aaatggtcgg catttgcgca ccagaccatc
    16321 cgcttgccgc caaaaacgtt tggacggcgg aagactttat cggggaaacc ctgattactt
    16381 atcccgttcc cgacgagatg ctggatttgc ccaaaaaaat cctgattccg aaaaacatca
    16441 acccgccgcg ccgacacagc gaactgacca tcgccattat ccaactggtt gccagcagac
    16501 gtggcattgc cgcccttccc tattggacag tcatgcccta ccttgaaaaa ggctatgtcg
    16561 ttcaccggca gattaccgac gacggactgc aaagcaaact gtatgctgcc atccgcactg
    16621 aagatacgga caagagctat ctgaacaact tttgccaaat catacgtgaa cgcggttttg
    16681 cagatttgcc cggactgagc gaactggaat cggtctgacc ccttattcaa ccatacccgg
    16741 cagtttttct attttttcat gtatagtgga ttaacaaaaa ccagtacagc gttgcctcgc
    16801 cttgccatac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
    16861 actatactgt ttttgatttt tgcccaatct gtaatcttta gattgccaat ggggaaccgt
    16921 ctactacaaa taaaaaaccc tgcgataagc agggtttttt gaatttccaa cattaacgtt
    16981 tggagaattg ttttgcacgg cgtgctttgc gcagacccgg ttttttacgt tcgacttcgc
    17041 gggcatcgcg ggtaacaaaa ccagcttgag acaaggcggg tttcaacgcg gcatcgaagt
    17101 cgatcagggc acgggtaatg ccgtggcgga ttgcgccgga ctggccggtt tcgccgccgc
    17161 caacaacatt gactttgatg tcgaaagatt cggcgttttc agtcagaacc aagggttggc
    17221 gaacaaccat tcggctggtt tcccgtgcga agaattcgtc aacgggacga ccgtttacga
    17281 tgatttgacc tgtaccttta atcaggaata cacgagccac tgaacttttg cggcggcctg
    17341 tgccgtagta gtatttaccg ttcatgtcgc gtccttattt cagttccaaa actttgggtt
    17401 gttgcgcagc atgggcgtgt tccgcacccg catacacttt cagtttttta atcatggcgt
    17461 aacccagagg acctttgggc agcatacctt ttacagcttg ttccaaagcg cggcccggga
    17521 attgctcttg catttcgcgg aaggtgcgtt cgtagatacc gcctgggaaa ccggaatggc
    17581 ggaagtattt tttatcttcg aatttggcac cggttacacg cagtttgtcc gcattgataa
    17641 caatgatgta atcgccggta tcgacgtggg gggtgtattc aggtttgtgt ttgccacgca
    17701 gacggctggc gacttcggcc gcaacgcgac ccaagacttt gtcttgggca tcgatgacga
    17761 accattcgcg cttcacctcg tggggtttcg ctgaaaaggt tttcatagtg gaaatccaga
    17821 tagatataga aagttgtaaa ttttaaagac aggattcgat tttgtcaatc gcattaccgc
    17881 gttacggaag gattttccgg atttcggcag actgcatact gctttttcgg gcggggcggc
    17941 ggccaatgtg aaaaaccgca tcgttgcgat gcggttttga atgggaatcc ccgcgagagc
    18001 cgtttcggcc gaatccgctt gaaccttgct gacaaggcgg ctgcctcggg tagtttcggg
    18061 tgcgtccgca aaaggacgct cgcgcccact actgctcccg gcaaccttaa gcgaacttat
    18121 tggttcaaag gaatatatgc cttcgcggac accgcaggga aaaaggggtt attcctgcgc
    18181 caagcgggat agtgcttttt ggcaggcgtt gtccatatcg gctattttac gcgcaaaatc
    18241 gccgattgcc aaatcgccgc cgttcaggga ggttttcaac aggtcgtgga cgacgttgag
    18301 cgcggccata atgacgattt tttcgctgtc cgcgacgcgt ccgccttcgc ggatggcttc
    18361 ggctttgccg ttgagcattc cgactgcctg caacagtgtg tctttttctt ctgccggcgt
    18421 gttgacggtc agccgggcgt gcatgacttc gatgtggact tgttcgatgt tcatccttta
    18481 atccttattg ctgcgtttcc tgccgttggg ggaggcgcgc tgccagtgcg ctgatttttt
    18541 ccctgctctg ttcgagcagg ctgcggtatc gtgtattttc ttctgtcagg ctgtcaattt
    18601 tgttttgcag gtcttctttg agtttgccga cttggacgag cagggcttcg ctaagttcgt
    18661 cgacggcggt ttcgtgttcg agtttttgcc gctcgtgcgc ccgtttgagt tcggcgacgg
    18721 tttctttgag gcggcggttt tcgctgacga gggtttcgaa tttttgtacc agcgtataaa
    18781 cgctgctttc gagtttttcg atattttgtt tcataacctt acctgtccgt atgccgtctg
    18841 aaggcttcag acggcatctg tctgttgttt attcaaaacg cgcgctacgt tccatcagcc
    18901 tttcgacgac ctgctgaggg gtcatttcct tacggatgag ttgcagcagg gtttgggtaa
    18961 tcggcatatc gatttggtac ttacaggcag tattgaagac ttcttctatc gtgctgaccc
    19021 cttcggaaac gtgtccgatt tcgaccagca cctgatgcag ttccttgcct tctgccaaac
    19081 ccaagccgac gcggcggttg cgtgagagtg cgccagtgca ggtcaagatg aggtcgccga
    19141 tgcctgccag ccccatcatg gttttgggct gtgcgcccat tgcggaggca aggcgggtga
    19201 tttcagctaa tccgcgcgta accagtgcgg cacgggcgtt aagcccgtac tctagaccgt
    19261 cggacaatcc ggtggcaatc gccataacat tttttaccgc gccgccaacc gccacgccga
    19321 tgacatccgt gctgccgtaa agccgcatga ccgaagtgtt gagttgtggg acaagttcct
    19381 cgacccattc ttggttttcg gaagcgagga cgacggcaca gggcagttgt ttggcgagtt
    19441 cctgtgcaaa actcgggccg gagagtacgc cgattttttt attgtcgggc aatacttctt
    19501 tcaatacttg aaaggtcagc agccctgtat cctgctcgaa tcctttgcag gcggcgagga
    19561 cggggaggtg tcccgcgccg tactgtttga gcagctctgc gctgcttctc aatccggcaa
    19621 cggaggttac gataaggaca agtccgctgt ctttgagcgc gtctgccaaa tccgcacaca
    19681 cttccaaggt ttcgggaaag gaaaagccgg gcagtccgcg tttgttttca cgcgcttcct
    19741 gcatttggcg gacttggtct gcgttgcgcg tccacaggga tacgcggttg ccgtgttggg
    19801 aaaagtgcag ggcgagcgcc gtaccccacg aacctgcgcc gataacggta attttcattg
    19861 gtcgtctttc aacatatcac tgccgttcac tttaaaacaa tcggtgtttc tctgcaagtg
    19921 cggtcaggga aatgccgtct gaaaggcgtt cagacggcat tttgccccga tgcagcacta
    19981 tcagccggta ttgcgcaaac cttgcgccac gccgttgatg gtcaggtgaa ccatcagaag
    20041 ggcgtgcgga ttgtcgggct ctttgcgcag gcgtttgagc atggcgactt gcaaaccgtt
    20101 gagcgcgttc agatatggaa tcctcaaggc aagcgaacgg gcgaggctgc ggttgtcgcg
    20161 caaaagttct tcggtttgca gcaggtcgag cagggctttg cggctgcgcc ggtattcttc
    20221 cttgatcatc ccgaagatga cttttgcttt atcgggcgat tcgctcaagc cggcatagtt
    20281 ttccgccaag gtgatgtcag ttttcgccat cacttgctcc atattggaga gcatggcttg
    20341 gaagaacggg ttgctttggg cgtgttcgcg cagcgcggcc agtgtgtcgg gattgccttc
    20401 gcacaatgtt tccactgcgc tgccgaaacc gtaccaagcc ggcagcatga ggcggttttg
    20461 catccaggaa aatacccacg gaatcgcgcg caagtcttga atccgtgcta aggtcttgcg
    20521 gctggcagga cggctgccga ggttgagggt agcgatttcc tgaatcgggc tggtttgcag
    20581 gaaatagtcg atgaagtcgg ggtgggtaat cagttcgcgg tagtatttga acgatacgtc
    20641 cgataaatcc tgcatcagtt tggcatcagg gtctttttta tccggcagga tgctggcttc
    20701 caaagtcgcg gcaaccaagg tttccaagtt gcgttgggcg ttgcccgggt cggcgtattt
    20761 ggcggtaatg acttcgcctt gttcggtgat gcggatttgt cccgccacgc tgcccgccgg
    20821 ttgggcgaga atggcttggt aagacgggcc gccgccgcgt ccgacgctgc cgccgcgacc
    20881 gtggaacagg cgcatgcgga catcgtattt tttgaagagt tcgaccaagc ccaattccgc
    20941 ctgatacaga caccatgagc tggtaacata gccgccgtct ttgttggagt cggagtagcc
    21001 gagcataatt tcttggatgt tgccgcagct ttcgagcagg gcatcgtacc aatcgaggcg
    21061 gaacatggtt tccatgaccg gacaggcgtt ttcgagtgct tcgatggttt caaacagcgg
    21121 cacgatattg atgcggctgc gcggtttgcc gttttccacc gccagcaggc cgctttcttt
    21181 cagcagcaat gccaaggcga gcaggtcgct gggttgttcg cagttggaaa taatgctttg
    21241 tgtaacggca tcttcgccga attcgtcttt gattttgcgc gcttcgttga aaattgccag
    21301 ttcgtggcgg gtgtgttcgc tgtatgtgat gaacgggctg tacagcggac gttgatggct
    21361 caattctcgc aacaggacgg cttgtttttg ttcttcgttc aggctgttgt agtcttccaa
    21421 gcctgcgtgt tggaaaagct cggcaactac atcggcgtgt ttgcctgcgt gttggcgcag
    21481 gtcgagcggc atcatgtgga agccgaacac ggatacgctg cggataatgt cggcaagacg
    21541 gccttcggca agcaggcggc tgccgttgtc gataagggaa cgttgcaatt ttttcaaatc
    21601 atcgagaaat tcttgtgccg aagcataagg ctcgagaaag ccgaatttgc agcccatgcc
    21661 caaaccgagc gcgcgcgctt tgcccatagc gcgcgccata atgtaggcaa tggcgcggcg
    21721 gtagggttct tccgtgcggg caagttcttc gtcaggcgag agggctgcca gtgccattac
    21781 gtcgtcgttg actttgacgc ggcggatgga gagcggcagt tcgcggtaaa gtttgtcgag
    21841 ttcgctgcgg taaaaacgga acacggcatc ggcgtggcgg cggaaggcaa aacgcaaggt
    21901 ttcggcagaa acgaacggat tgccgtcgcg gtcgccgcca atccagccac cgattttgag
    21961 gatattcgga acgcagacat cgggatagac catctgaaag tcgtgttcca tcttgcggta
    22021 gagcttgggc agggcttcga aaaagctcat cgggaagatg gacacgccgt tgttgatttc
    22081 gtcgttgacg ctgagtttgt ggcggcgcgt ttcgctggtc tgccacaagc ccagcaggat
    22141 agtgtcgatt tcgcggcgca gccgtgccag cgcgtcggca ttggtgcagc gttcgcgttg
    22201 cggcaacagt gcgcggatgc ggcggttgaa gcttaagacg gtttggcgtt gcacttcggt
    22261 cgggtgcgcg gtcaaaacgg cggtaacgga cgtattgtcc aactgccgct gcaccgattt
    22321 gccgtcggct ttccccgctt tgagcctgcg gacggtttcc gtcaggctgc cttccgcgcc
    22381 gccgcgtcca gcttcttcgt ggatttggcg gcggcgttcg tggtgcacgt cttcggcgat
    22441 gttcaaaatc tgggcgaaca ggccgcaggc caaggttaaa tcgtgggttt gttgttcgtc
    22501 caattgcggc aatacttttt caatcaatgc cgcgctgtcg tcggaagtgg acaagagttt
    22561 gaccgtttcg acaaccaacg gcgaggcttc ttcgtgcagg aggttgaaca gggattgttt
    22621 cagaaattcc gcgtccgccg ccaaagccgc gtccttcgga ttgttcagaa tatgcagttg
    22681 catgattttt ctctcattgc cgtaaatact gtaaatgtac ctcaaatgcc gcatccgtgc
    22741 caaaccgttc acactttaac cgcccgtgtc ccgaaatgcc gtctgaagtt gaacgccgcc
    22801 cgacggcagc gttacaatcg cccgcaactg ttttttccga acatcatcat gaccacgacc
    22861 aaacacgaca acgacgacga actcctgctg cggtacagcc gccacatcct cttggacgaa
    22921 atcggcatcg aagggcagca gaaactttcc ggcgcgcata ttttggtcgt cggctgcggc
    22981 ggattgggtg ccgccgccct gccctacctt gccgcttccg gcatcggcac gctgaccata
    23041 gccgatttcg acacggtcga actgcacaac ctgcaacgtc aggtcgcatt taacgagggc
    23101 gatgtcggca agcccaaagc cgaagcctta gcaggccgtc tgaaacgcat caaccatacc
    23161 gtcaacgtcc gcaccgtcaa cgaaaaactc gacggctgcc gcctgaccgg tttggttcaa
    23221 accgccgaca tcgttttaga ctgctgcgac aactacgcca cgcgccaagc cgtcaaccgt
    23281 gcctgcgtac aaacgaaaac accgctggtt tcaggagcag cggtgcgctt tgaagggcag
    23341 cttgccgtgt accgtcccga cttgcccgac tcgccgtgtt atgcctgcct gttcgacggc
    23401 ggatcggctt cagacggcat ctgttccctt ttcggcgtat tctcgccgct ggtcggcatc
    23461 atcggctgca cgcaggcggc ggaggcactg aaaatcctgc tggatgcagg cgaaccgtcg
    23521 cacggcaggc tggcggttta ccgtgctttg gaagggggct ggcaatattt cgacctgccg
    23581 cgcaaccctg aatgcccggt ttgcggcgcg gaacggtaaa accctgccgc cgtttcagac
    23641 ggcataaaaa cggattcggc ggaacggttt taaaaatcgg cggtgttttg acatagtgtc
    23701 gataagcata cttatcttca atatactcaa caggataatt gtttgcctaa gcaaaaaaat
    23761 aatcgccttt tccctgacgg gcgggcgaaa tccacggatt gcaaaaacac aacctgaaaa
    23821 tttacatttc tttgcaaaaa aaaaaaaaaa aaataaactt accttataat tgcaattgtt
    23881 ttagcaatat ctgattcgca gattcattga gttaagatat tttccccgta atgtgtttgg
    23941 ccgtctgtcc cttttgggtt cggacggctt tttttaggct gtgtttgaat acccagttga
    24001 tttcgtctgt ttgtaggggg aaagccactt atttccgttc gggtggaaac gctcccatcg
    24061 gataaaaggc attttgtccg actgattaaa
//
LOCUS       DAWKQB010000014        23699 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.014, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000014 DAWKQB010000000
VERSION     DAWKQB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.014"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(107..946)
                     /locus_tag="WJL14_000431"
     CDS             complement(107..946)
                     /locus_tag="WJL14_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7655556.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAVDLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1231..1306
                     /locus_tag="WJL14_000432"
     tRNA            1231..1306
                     /locus_tag="WJL14_000432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1264..1266,aa:Arg,seq:ccg)
     gene            complement(1371..2564)
                     /locus_tag="WJL14_000433"
     CDS             complement(1371..2564)
                     /locus_tag="WJL14_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7655557.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHCGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2585..3673)
                     /gene="trmA"
                     /locus_tag="WJL14_000434"
     CDS             complement(2585..3673)
                     /gene="trmA"
                     /locus_tag="WJL14_000434"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ7655558.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDDEWMQAAQALQQQLDTSVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGGFTQPNAAVCQKMLEWACGAAEGLGGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVGAAQWNIEANGIGNIKIARLSAEEFTEAYTGKREFARLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     VVERAALFDQFPFTHHIESGVLLKKKIL"
     gene            3820..4920
                     /gene="aroC"
                     /locus_tag="WJL14_000435"
     CDS             3820..4920
                     /gene="aroC"
                     /locus_tag="WJL14_000435"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ7655559.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            5012..5437
                     /locus_tag="WJL14_000436"
     CDS             5012..5437
                     /locus_tag="WJL14_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ7655560.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAVAETPSVEAEAAESSTTE"
     gene            complement(5511..7496)
                     /gene="parE"
                     /locus_tag="WJL14_000437"
     CDS             complement(5511..7496)
                     /gene="parE"
                     /locus_tag="WJL14_000437"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ7655561.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFFKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7563..8087)
                     /locus_tag="WJL14_000438"
     CDS             complement(7563..8087)
                     /locus_tag="WJL14_000438"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ7655562.1"
                     /translation="MLDREGYRPNVGIILINERNEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            8252..9547
                     /gene="serS"
                     /locus_tag="WJL14_000439"
     CDS             8252..9547
                     /gene="serS"
                     /locus_tag="WJL14_000439"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ7655563.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQVAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9620..10618)
                     /locus_tag="WJL14_000440"
     CDS             complement(9620..10618)
                     /locus_tag="WJL14_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7655564.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPSTPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10743..11819)
                     /gene="prfA"
                     /locus_tag="WJL14_000441"
     CDS             complement(10743..11819)
                     /gene="prfA"
                     /locus_tag="WJL14_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ7655565.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11923..12702)
                     /locus_tag="WJL14_000442"
     CDS             complement(11923..12702)
                     /locus_tag="WJL14_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7655566.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATRPQDW
                     QDVADLAQRSSENPILGNIHIALGIHPWFSDDVSEPDFQRLEQALKARPTAWVGEIGL
                     DFYDKTQTPQQRERQIQVFSRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGI
                     VHAFSGSAEEARVLAKLGFKIGIGSLLLNPNARKIRETLKTLNDTDFVLETDSPFMLK
                     KEINTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12710..13702)
                     /locus_tag="WJL14_000443"
     CDS             complement(12710..13702)
                     /locus_tag="WJL14_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ7655567.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAAVVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTRQGKLLLNISQVRQGK
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGAEDVEKELVRLKLV"
     gene            complement(13769..14452)
                     /locus_tag="WJL14_000444"
     CDS             complement(13769..14452)
                     /locus_tag="WJL14_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7655568.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGVMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14640..15974)
                     /gene="glmM"
                     /locus_tag="WJL14_000445"
     CDS             complement(14640..15974)
                     /gene="glmM"
                     /locus_tag="WJL14_000445"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ7655569.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRINKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARNGAERIAAAIGSL"
     gene            complement(16104..16961)
                     /gene="folP"
                     /locus_tag="WJL14_000446"
     CDS             complement(16104..16961)
                     /gene="folP"
                     /locus_tag="WJL14_000446"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ7655570.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRLGADYVSPEEEWARVEPVLEEVAGWGVPVSLDTRR
                     TVIMEKALALGGVDIINDVAALNDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKTLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(17101..17994)
                     /locus_tag="WJL14_000447"
     CDS             complement(17101..17994)
                     /locus_tag="WJL14_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ7655571.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVCIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18085..20199
                     /locus_tag="WJL14_000448"
     CDS             18085..20199
                     /locus_tag="WJL14_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ7655572.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIARTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDQTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20263..21741)
                     /gene="ubiD"
                     /locus_tag="WJL14_000449"
     CDS             complement(20263..21741)
                     /gene="ubiD"
                     /locus_tag="WJL14_000449"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ7655573.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVIGMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVDVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWLGETDREWGSVIKKDPAVTAKIDGIWEELGL"
     gene            complement(21761..22276)
                     /locus_tag="WJL14_000450"
     CDS             complement(21761..22276)
                     /locus_tag="WJL14_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ7655574.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSRFYDNPENAVYF
                     GMICNRLPPYESDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSKSDCSG"
     gene            complement(22283..22782)
                     /locus_tag="WJL14_000451"
                     /pseudo
     CDS             complement(22283..22782)
                     /locus_tag="WJL14_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23014..23583)
                     /locus_tag="WJL14_000452"
                     /pseudo
     CDS             complement(23014..23583)
                     /locus_tag="WJL14_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23623..>23699
                     /locus_tag="WJL14_000453"
     CDS             23623..>23699
                     /locus_tag="WJL14_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7655575.1"
                     /translation="MLPSKYEDNTLQIKEKSTERTAPFFV"
BASE COUNT         5384 a         6743 c         6174 g         5398 t
ORIGIN      
        1 gcttcgtcgc cttttcctga tttttgttaa tccactataa attcccataa aaaaacggag
       61 cagatacctg ccccgttttt atttaatccg aaattttaat ctaaatttag aattttgcac
      121 cggattggtt tgccatatag tcaacagccg ctttgacttc gtcatcgctc aaacctgcat
      181 tgccgccttt ggcaggcatc gcgttaaagc cttcaagggc gtgtttgtgc aaggtttctt
      241 tgcctttttt gatacgcggt gcccaatcgt cttttttgcc tatgccggga ataccgggaa
      301 tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac ttttttaccg tcaacgccga
      361 ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc tgccggagct gcactatcgg
      421 caggagcaga agctgttcct gaagcggcat tgtcggcagg agcagcctca tcaggattcg
      481 ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc ccgtttgagt tcctgatcgg
      541 tcaggtctac cgcaccgcct ttggcaggca tggcgttaaa gccgttcagc gcgtgttgga
      601 acaaggtatc gaagccttgc gcgatacgcg gcgcccaatc gccgttgtgt tccagtttcg
      661 gagcgttcgg cacattgctg tccgccgcgt ggcattggat acagattttg ccgaaaatct
      721 gttcgccttg gcgttcgccg acggggatgc cgtcgcccat cgtcaattgt ccgacaggct
      781 ggatacgggt ctgcgttgct gcttccgtag tggcatcgac atcgccgaac gagccgctgc
      841 ccgccagctt aatcaggaaa taaaggactg caataacaat aacgataccg ctcacaaggg
      901 taaacagtgc agagccttgg gctttgttgt cgcggagttg tttcatttgg taggcctcgc
      961 cgtcaggtta ggttgtgctg taaattatag tttggtgtgt taaacgcagt taacaatatt
     1021 ttgctggatt atactgaatt cacagggtct ttccaatcgc tatcattgaa aatatgaaaa
     1081 aatttgccaa cggtatctgt ataaaacaaa taatcctttg aaaataatgg tttatcctca
     1141 agaaaactct ccttatgccg ccatacgccg cctgccggcg caagataacc tttgccaatt
     1201 tgcagaattt acgttaacct tacgttttac gcacccatag ctcagttgga agagtgtcag
     1261 tttccgaagc tggaggtcac aggttcgatc cctgttgggt gcgccaatta taaagagacc
     1321 gtctgaaaga taaatatttt tcagacggtc ttttgactta cttcaaactc ttatttcaag
     1381 acttccgcaa atgcgcgggc aacatagtcg gtattcgacg tattcagtcc ggcgacgcac
     1441 atcctgccgg aatccagcag gtaaacggca aattcgtcgc gcagcctgcg gacttgttcc
     1501 acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga tgaaataagt gaaatcgcga
     1561 tcggggattt gcgcagttaa gacatcataa agtttctgcc gcatcgcacg gatgcggtcg
     1621 cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt cggggctgtt catcacgtcg
     1681 gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga tgcggcggac ggtgaatttg
     1741 agctgtccga acaccaaatc cgcttcttcc ttattcgggc aaaccacgct taagccgccg
     1801 acgcgctcgc cgtagagcga caggtttttt gagaaggaat tgctgacgaa caagggcaat
     1861 tccatttcca ccgctttgcg gacggcgtag gcatcgctgt ccaaatcgcc gccgaagcct
     1921 tggtaggcga tgtccataaa cggaatcagt ttgcgcgttt tgatgatgtg caacacttcg
     1981 tcccattgtt gttccgacat atccacgccg gtcgggttgt ggcagcaggg atggaggatc
     2041 aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca tttcgtcgaa tttcacgccg
     2101 acagtggcag ggtcgtaata tgggtaagtg ccgacctcga aacctgcgcc ttcaaaaatg
     2161 ccgcaatggt tgtcccaagt cgggtcgctg acgtaggcgc gcgcttcagg aaaccagcgg
     2221 tgcaggaaat ctgctccgac tttgagcgcg cccgagccgc ccaaggtttg tacggtagcg
     2281 atgcgtcctt gcgcgagcgc agggttgtct ttgccgaaca ataaatgctg caccgcgctg
     2341 cggtaagtgt ccaagccttc catcggcagg tagggcgacg gcgcaggcgc ggcggcacgg
     2401 gcggtttcgg ctcggctcac ggattccaac acgggcattt tgccttcgtc gtcgaaataa
     2461 atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt ttttgaaggt ttcgaccaaa
     2521 ctcaaaatcg ggtcgccggg atagtattcg atgtgtcggt acatagtcct tacctcttgc
     2581 tttttcaaag gattttcttt ttcaacaata caccactttc gatatggtgc gtaaacggga
     2641 attggtcgaa cagggcggcg cgttcgacca catgggtttc caccaaggtg tccaaattgg
     2701 cacgcagcgt ttcgggattg caggaaatgt agatgatgtt gtcaaactgc gacaccagct
     2761 tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac gaaaatagtg gaaaatgcgt
     2821 aatccgtcaa agcaataccg ccatccttaa ggcgtgcaaa ctcacgtttg ccggtatagg
     2881 cttcggtaaa ttcctcggca gacagacggg cgattttgat gttgcctatc ccgtttgcct
     2941 caatattcca ttgcgccgca cctactgaag ttttggaaat ctcggttgcc aaaacacgtt
     3001 caaacttttc agataaaggc agggtgaaat tgccgttgcc gcaatacagt tcgagcaggt
     3061 cgccgcccaa gccttccgcc gcaccgcatg cccattcaag cattttttga cacacggcgg
     3121 cattgggttg ggtaaaaccg ccttcaattt gccgataacg aaaatcccga ttaccgacct
     3181 ttaaagtttc cgttacatag tcctgtttca agactatttt ctgccccctg ctccgcccaa
     3241 taacggaagt atccaactgt tgctgtaacg cttgcgccgc ctgcatccac tcatcatcaa
     3301 gccttttgtg gtaaatcatg gtaaccagca tttccccgct gagcgtagac aaaaattcga
     3361 cggcatacca acggtttctc agttcggcag attgggcggc aacggcaatc aattctggca
     3421 tcaggcggtt gacggcttcg gaagccgctt caaaacggtc gcaacggatc aggcttgccc
     3481 cgccagcctt ttgtcctttt tcaaacatgg cataaaacat ttcaccgcct tcgtgccaaa
     3541 tacggaactc ggcacgcata cggtaatgtt tgtccggaga ttcgtacact tcccactcag
     3601 gaacatccaa acctgcaaaa agggttttaa ggtggtcttt tttatcttga agctgctgcg
     3661 tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga ttataagccg atttgcctcg
     3721 ccgcacgcaa actctttgac tgccgcccct tcaatgacgg acgggctttt gtgctaaaat
     3781 ccgccatctt tccacactat accgataaag ggaaaaatca tggcaggcaa cactttcgga
     3841 caactcttca ccgttaccac cttcggcgaa agccacggcg cgggtttggg ctgtatcatc
     3901 gacggctgcc cgcccggctt ggaattaagc gaagcggata tccaatttga cctcgaccga
     3961 cgcaaacccg gcaccagccg ccacgttacc caacgccgcg aagccgacca agtcgaaatc
     4021 ctctccggcg tattcgaagg caaaaccacc ggcacgccca tcgccctctt aatccgcaat
     4081 accgaccagc gcagcaaaga ctacggcaac atcgccacca gcttccgccc cggccacgcc
     4141 gactatacct attggcacaa atacggcacg cgcgactacc ggggcggcgg caggagttcc
     4201 gcccgcgaaa ccgccgcccg cgttgccgcc ggagccgttg ccaaaaaatg gttgaaagaa
     4261 aaattcggca cggaaatcac cgcctacgtt acccaagtcg gcgaaaaaga aatccggttt
     4321 gaaggctgcg aacacatttc ccaaaatcct ttttttgccg ccaaccatag ccaaattgcc
     4381 gagctggaaa actatatgga cagcgtgcgc aaatccttgg attccgtcgg cgcgaagctg
     4441 catattgaag ccgccaacgt ccccgtcggc ttgggcgaac ccgtattcga ccgcctcgat
     4501 gccgaaatcg cctacgccat gatgggcatc aacgccgtca aaggcgtgga aatcggcgca
     4561 ggttttgaca gcgtaacgca acgcggcagc gaacacggcg acgaactcac cccgcaaggc
     4621 ttcctgtcca accactcagg cggcatcctc ggcggcatca gcaccggaca agacatccac
     4681 gtcaatatcg ccatcaaacc caccagctcc atcgccaccc cgcgccgcag tatcgacatc
     4741 aacggcaacc ccatcgaact cgccacgcac ggcaggcacg acccctgcgt cggcttgcgc
     4801 gccgcgccga ttgcagaagc tatgctggca ttggttttaa tcgaccacgc cctgcgccat
     4861 cgcgcgcaaa atgcggatgt tcaggttaat acgcccgaca ttaccctttc aaacaaataa
     4921 aaatttagcc aaaacacaga ctttataata gaataccgag cattgccgtg ccgctttgac
     4981 gcacgggttt gtttagagga atacaaccga aatgacacaa gaaaccgctt tgggcgcagc
     5041 actgaaatcc gccgtccaaa ctatgagcaa aaagaaacag acagaaatga ttgccgacca
     5101 catctacggc aaatatgatg tattcaaacg atttaaaccg ttggcactcg gtatcgatca
     5161 ggatttgatt gccgctttgc cccaatacga tgccgcactg attgcacgcg tcctcgccaa
     5221 ccactgccgc cgtccgcgct atctgaaagc cttggcgcgc ggtggcaaac gtttcgattt
     5281 gaacaaccgt ttcaaaggcg aagttacccc cgaagaacag gcaatcgcgc aaaaccatcc
     5341 tttcgtgcag caggctttgc aacagcagtc cgcccaagct gtcgccgaaa cgccgtctgt
     5401 tgaagccgaa gcagccgaat cttccacaac agaataaatc cccaaacgaa atgccgtctg
     5461 aaagccgatt tggtttcaga cggcattttt tcgtatgcgg caatcacggt tcaaatatcc
     5521 aattccgccg tatcgccttc gcgttccatc caagcgcggc gggcggcggc ttcgcctttg
     5581 cccatcagtt tgaagaagat gtcgcgcgtc tcgtcatccg cgccttcggg gatttgtacc
     5641 tgcaacaggc ggcgggtgtc ggggtgcatg gtagtatctt tgagctggtc tgggttcatc
     5701 tcacccaaac ctttgaaacg gctgatggaa taagcagttt ctttaacgcc ttctttttgc
     5761 agccgctcta aaatgccgtc aagttcgttt tggtcgaggg cgtagaattt gcgggcgggt
     5821 ttgcttttac cttgtgcgtt gacatctacg cggaacagcg gcggctgggc gacgtagatg
     5881 tgtccgtcag cgaccagttt cgggaagtgg cggtagaaca gggtcagcag caaaacttga
     5941 atatgcgagc cgtccacgtc ggcatcggac aggatggcga ttttgccgta acgcaggccg
     6001 cttaaatcgg gatggtcgtt gatgccgtgc ggatcgacgc cgatggcaac tgaaatgtcg
     6061 tggatttcgg cgttgccgaa gagctggtcg gggtggactt caaagctgtt gagcactttg
     6121 ccgcgcaggg gcaggatggc ctgggtggct ttgtcgcgag cgagtttggc ggagccgccg
     6181 gcggaatcgc cttcgacaag gaagagttcg ttttcgcgga tgtcttcgct ttcgcagtcg
     6241 gtcagcttac cgggcaggac ggcgacgccg ctgccttttt tcttttcaat ctttttaacc
     6301 gaacgcatcc gcgcctgtgc ctgacggatg gcgagttcgg cgattttttt gccgaagtcc
     6361 acgttttggt tcagccacag ttccaaaggg tcgcccgata cggtagcaac gagtttcagc
     6421 gcgtcgcggt tggtcagttt gtctttggtc tgaccttgga actgcgggtc gaggacacgg
     6481 gcagagagga caaaggcagt tttgccgaac acgtcgtcgc tttgcacttt cacgccgcgc
     6541 ggtaagaggt tgtgcaggtt gatgaagttg ctgacggcgt tgaacacggc ttgtttcaag
     6601 cccgcttcgt gcgtgccgcc cagcggggtg gggatgaggt tgacatagct ttcgttggca
     6661 cacgagcctt cttccagcca agtcagggca aaggcggcgc cttcgccgat gctgaaatcg
     6721 ccgttgtgtt cgtctgaaat gtagttttcg caagagaaca gcggtacggc ttcctgcgcg
     6781 tcggcaatca ggtcggtcag atagcttttc aggccgtcgg ggtaatgcca ggtttgggtg
     6841 tgcgcttcgt cttcgccttt gaccggacgg gtcagggaaa cgcgcacacc cggcagcagc
     6901 acggctttgg cacgcagcag gcgttcgagt tcgggaatgc tgtaattcgg gctttcaaaa
     6961 tatttgccgt ccggccagac gcgcacttcg gtgccgctgt ctttgacggc gcatttgccc
     7021 acttgtgcca acggttcgac cacgtcgccg ccggcaaaca cgatacggtg gattttgcct
     7081 tcacgtttga ccgttacctc aaggcgggtg gagagggcgt tggtgacgga tacgcccacg
     7141 ccgtgcagtc cgcctgaaaa ggcatacgcg ctgccgccgt cttttttgtt gaacttgccg
     7201 cccgcgtgca gacgggtgaa cacgagttcg actacgggta cgccttcttc agggtgcagg
     7261 ccgacgggaa taccgcggcc gttatcacgg acggaaagcg aaccgtcttc atgaatttgc
     7321 acgtcgattg cagtcgcgaa accacccaac gcctcgtccg ccgcattatc aatcacttcc
     7381 tgacagatgt gggtcgggct gtcggtgcgg gtgtacatgc cgggacgttc tttgaccggc
     7441 tccaagcctt tgaggacggt gatgctggat tcgctgtatt ggttgttttt agccatagga
     7501 ataatctgaa agtaagaaaa acaacgcttt cagacggcct gaaagcgttg cgttccgttg
     7561 ttttagcggt tgtcggaaga ttggcgggcg gcaaagtctt cataactttc cataccgcgc
     7621 aggaagcggg aagagagttc tttcaacgcc cccaaataaa cgtcgcgttt gaaatcaatc
     7681 acttcgtcaa cgggcgccca atattgatgc caacgccagc cgtcaaattc ggggtggcgg
     7741 gtggcgcgca ggttgacatc gcaatctcgg ccggtcaggc gcaggagata ccaaatctgc
     7801 ttctgcccgc gataggatcc gcgccattcg cggcgtaccc agttgttcgg cacgtcataa
     7861 cgcagccagt cgcgcgtgcg gccgataatt ttgacgtgtt gcggcaaaag cccgacttct
     7921 tcgtacaact cgcggtacat ggcggtttcg gggctttcgc ccggcttgat gccgccttgc
     7981 ggaaactgcc aagaatgttc gcgcacgcgc ttgccccaaa agacttcgtt acgttcgtta
     8041 attaggataa taccgacatt ggggcgatag ccttctctgt ccaacacggt gtcgccctcc
     8101 gttaaattca atcttgggat tttcccacaa atcgaacggt tttgacaaat cggacggtat
     8161 ggttcaagca tgtcgaaaca cagacggact cgggaaaata tcttaaattt agtttacaat
     8221 aatacgtttc aaattattcg ggaatcagac catgttagat atccaattgc tccgcagcaa
     8281 caccgccgcc gttgccgaac ggcttgcacg gcgcggttat gactttgata ccgcacgttt
     8341 tgacgcactg gaagaacgac gcaagtccgt tcaggtgaaa accgaagaac tacaagcctc
     8401 gcgcaacagc atttccaaac aaatcggcgc tctgaaaggt cagggcaaac atgaagaggc
     8461 gcaggtggcc atggatcaag tcgcccaaat caaaaccgat ttggaacagg ctgccgccga
     8521 tttggatgcc gttcaaaaag aattggacgc atggttgttg agcattccga acctgccgca
     8581 tgaggacgtg cctttcggta aggatgaaac cgaaaacgtc gaagtccgca aagtcggcac
     8641 gccacgcgaa tttgactttg aaatcaaaga ccatgtcgat ttgggcgaac ctttgggttt
     8701 ggatttcgaa ggtggcgcga aactttccgg cgcacgattt accgtgatgc gcggacaaat
     8761 cgcccgcctg caccgcgcgc tggcgcaatt catgctcgat acgcacacgc tgaaacacgg
     8821 ctacaccgag cattacacac cctatatcgt ggacgacacc accttgcaag gcacgggtca
     8881 gctgccgaag tttgcagaag atttgttcca cgttacccgt ggcggcgacg agagcaaaac
     8941 cacgcaatat ctgattccga cagctgaagt taccctgacc aataccgttg ccggcagcat
     9001 tatcccgtcc gaacaactgc cgctgaagct gaccgcgcat tcgccctgtt tccgcagcga
     9061 ggcgggttcg tacggcaaag acacgcgcgg tctgattcgc cagcaccagt tcgacaaagt
     9121 ggaaatggtt caaatcgttc atcccgaaaa atcatacgaa acgctggaag aaatggtcgg
     9181 ccatgccgaa aacatcctga aggctttgga actgccctac cgcgtgatta ccctgtgtac
     9241 cggcgacatg ggcttcggcg caaccaaaac gtatgatttg gaagtttggg taccggcgca
     9301 aaatacctac cgcgaaatct caagctgttc caactgcgaa gatttccaag cccgccgcat
     9361 gaaggcgcgt ttcaaagacg aaaacggcaa aaaccgcttg gtacatactt tgaacggctc
     9421 cggcttggca gtcggcagaa cgctggtcgc cgtattggaa aaccatcaaa acgccgacgg
     9481 cagtatcaat atcccagccg ctttgcagcc ttatatgggt ggcgttgcca agttggaagt
     9541 gaaataagcc ttaaccctga acataaatgc cgtctgaaac ctgtttcaga cggcatttcc
     9601 tttaaactct taaaacacgt caaccgtcgg cacgaaccgc attgccgcaa tcgccggtct
     9661 gtccgacctc gcggatattg gacagcgtaa cttccgaaat attacccaac gcctcttccg
     9721 tcaaaaatgc ctgatggccg gtaaacagca cattatgaca agacgacagg cggcggaaca
     9781 cgtcgtcggt aatcacatcg ttggatttgt cttcaaaaaa caattcgcgt tcgttctcgt
     9841 acacgtccat acccaatgcg ccgattttcc ggcgtttcaa cgcctcaatc gcggcggcac
     9901 tgtcaatcag cccgccccgg ctggtgttga taatcatcac tccgtctttc attttgtcga
     9961 acgccgcttc gttcagcata tagtggtttt ccggcgtgga ggggcaatgc agcgtgatga
    10021 tgtccgactg ggcatacagc tcgtccaaat ccacatacct gccgccgatt ttttccgctt
    10081 cggggttgca aaacggatcg tgagccagca ggttcatacc gaaacccttt aaaatccgca
    10141 tggttgcaat accgattttc cccgtgccga taacgcccgc cgttttgccg tacatattga
    10201 aaccggtcag accttccagc gaaaaattcg catcgcgggt acgctgatag gctttgtgga
    10261 tacggcggtt caacgtcagc atcagaccga ccgtatgttc cgcaaccgat tcgggcgaat
    10321 aggcaggcac gcgcacgact ttcaagccca actcttcagc cgcctttaaa tccacattat
    10381 tgaagccggc acaacgcaac gccacagttt tcacgccaat ttgcgccaat ttttccaaca
    10441 cgggccggct gccgtcgtcg ttcacaaaaa tacagaccgc ctccgcacct tccgccattt
    10501 tcgccgtttt cgcatccagc ataaaatcaa aaaactccag ctcgaagccg aaatgccggt
    10561 tggcgcgggt aaaatgttcg cggtcatagc ttttcgtacc gtaaatcgca atcttcatca
    10621 atatgtccag ttgtcgtcta tggttgagaa acggcataat acactgaatt caaacaaatc
    10681 agtagaatat ggtggattaa aattgattgc atgcacggca tttccatttc aaaacacaaa
    10741 actcaatcgc ccattgccgc caaaagctcc gcctgatgtt cggcaatcag ggcatcggtg
    10801 atttctgcca aatcgccgtc catcacaaaa tccagcttgt gcagggtgag gttgatgcgg
    10861 tggtctgtta ccctgccttg gggatagttg taggtgcgga tgcgttcgct gcggtcgccg
    10921 ctgccgatga gggatttgcg ctcggcggct tctttggctt gggcttcgcg tttttgcgcg
    10981 tcgttcaggc gggcggcgag gactttcatt gcctgcgctt tgttggcatg ttggctgcgg
    11041 ccgtcttggc attcgaccac catgccggtg ggcaggtggg tgatgcggac ggcggagtcg
    11101 gttttgttga tgtgctgacc gcccgcgccg gatgcgcgga aggtgtcgat gcgcaggtcg
    11161 gcggggttca gttcgatgtc ttccagttcg tccgcttcgg gcatgacggc gacggtgcag
    11221 gcggaggtgt ggatgcggcc ttggctttcg gtggcgggga cgcgctgcac gcggtgtccg
    11281 cccgattcaa atttcaaacg gctgtacgcg cctaagccga taatacgggc gataacttct
    11341 ttatagccgc ccaactcgct ttcgttggcg gaaacgattt cgacctgcca gcggttacgc
    11401 tcggcgtagc ggctgtacat acgcagcaaa tcgcctgcaa acaacgcggc ttcgtcgcca
    11461 ccggtaccgg cgcgtacttc gataaagatg tttttgtcgt cgtcggcatc tttgggcagc
    11521 agcagttttt gcagttcggt atcgagttcg ccgattttgg ctttggccgc ttcgatttct
    11581 tcggcggcaa agtctttcat ttcggggtcg gacaacattt cttcggcatc tgccaagtcg
    11641 ctttgcgcca gttgatagtt ttggaacact tcgaccacgg gcgtcaattc agcgtgttcg
    11701 cgggtaagtt tgcggtaatt gtccatgtca gacgtggctt cgggttgccc taaaaggtgg
    11761 gtaacttctt ccaaccggtc gccgagttgt tgcagttttt ctaagataga cggcttcata
    11821 attcttccat aacaaacgcc gcctgaattt cagacggcat caaaacgcgg tcattataat
    11881 atattttctg aatattcaaa aagataatct tagatggata acctaccgtc ccaacagggc
    11941 atcggcattg cgctccgtta ccttggcaat ctcttctaca caggttccgc ggatttccgc
    12001 agcaatcttt gcaatacccg gaatattggc aggcgtatta atctcttttt tcagcataaa
    12061 cgggctgtcg gtttccaata cgaaatcggt gtcattcagg gttttgaggg tttcgcgtat
    12121 tttgcgggcg ttcgggttga gcaacaacga accgatgccg attttgaaac ccagtttcgc
    12181 caacacgcgc gcttcttccg cgctgcctga gaaggcgtga acgatgccgc cttgggcaaa
    12241 gcctgtctgt ttgacggcgg cggcgatgtc ggcggtggct ttgagattat ggataatcac
    12301 gcggcggcgc agggtttgcg cgatggcaag ctggcggctg aaaacttgga tttgccgttc
    12361 gcgctgctgc ggcgtttggg ttttgtcgta aaaatccaag ccgatttcgc cgacccatgc
    12421 cgtcggacgc gctttcaatg cttgctccaa acgctggaaa tccggttcgg aaacgtcgtc
    12481 tgaaaaccag ggatggatgc cgagtgcgat atgaatattg cccaaaatcg ggttttcaga
    12541 cgacctttga gccaaatccg ccacgtcctg ccaatcctgc ggacgggtcg cgggaacaat
    12601 aaaccgcttt accccgactt tccgcgcggc ggtcaggatg tgcggcaggt tttcgcgcaa
    12661 ggcggcatcg gcgaggtggc agtgggtgtc ggtgaagttc attccggttt cacactaact
    12721 tcagccttac caattctttt tctacatctt ccgcgcccca gccttgcgat acggcgaggg
    12781 tcataagcgc ggtggcggtt tcgaggttgc atttgccgcc gttgatgatg cccgagttgc
    12841 ggaacgcgtt gccttgcgcg taaacggcag cggttttgcc ttgtcggact tggctgatat
    12901 tgagcagcag tttgccttgc cgcgtgaagt cttgcacggc gcggataaag ccttcgtctg
    12961 ccggggtgtt gccgtgtccg tagctttgca ggataagggc ttgggcggga agctgtccaa
    13021 gtccgtccgc aagttcttgg acggcaaagc cggggataag cgtgcggacg gcgatttttg
    13081 cctgcgggtc aggatagcgg attttgaggc cgtctgaaac gactgctgcg tcctgattcg
    13141 ggatgcggac attgtgccag ccctgggttt cgtcccactc cgccagtgcg ccgaaatgcg
    13201 gattgtcgaa gcctgcggcg gtttcggtgc tgactttgct gctgccgacg gcgggataaa
    13261 gtttgccgtc aaaggcaata agcgtctgct tcaatccgag tgggaatgcg gcaacggcag
    13321 tcgccagatt gcgcggcgcg tcgctgccca cggcgttgta cggccattgg gaaccggtca
    13381 ggacgatcgg tttgtccaaa ccttgcaggg cgagggcgaa cagattggcg gtataggcga
    13441 gcgtgtccgt gccgtgcagc accaaaacgc cgtcgtactg cggcagcgcg gcggcaatga
    13501 tgtccagcca agcgcaccag ttttgcagtt ggacggcgga agaatcaatc agcgtcccac
    13561 aaacgtgcca atcgaaatcc atgccgtttg aaaacggcgc gagtgcctgc ccgaccagcg
    13621 cggtatcggg gcgcaggcct tcgctgcttt gcgtcatgcc gatggtgccg ccggtataaa
    13681 ggacaaaaat tttctgtttc atggctcatc ctgaaaatgc aaagaccgcc tgaaacgcga
    13741 aatgcgtccg gacggtcgaa aatctgattt actgctgctt ctgtgccgct tttttcgctg
    13801 ccttttccgc cttgcgtttg gcgcgttttt cgctcaattg tgcgcggtaa agctgataat
    13861 gtcggcgttt gcgccaccag aaccacgcca gcgccgccgc cagcacgccc aatgcgatga
    13921 agatgccgga ttgcaggctg tgcattttcg ccatcagcca atcgatgttg tgcgcgccgt
    13981 actcgcccaa gtaaatccaa acgggcacgg aaatcagcgc ggcaagcccg tccataatca
    14041 gaaagcgcag atacgatact ttgcggctga tgccggcggt aacgaaaacg gcagtccgca
    14101 aacccggcag gaagcgggcg acaaacagaa cccagttgcc gtatttgtcg aatttttcct
    14161 gaacctgtgc gtaacgtttc ggcgtcatga tgcgcgcaat cggtttgaac ttgaggattt
    14221 tctgccccca gatgcgtccg gcggcaaaca tcacgccgtc gcccgccaac acgccgagca
    14281 tgccgactgc aaacataata tgcggattgg tgtaacccat acccgaaatc acgccgcctg
    14341 ttaccaaggt caaatcctcg ggaatcggca cgccgaaacc gcagatgacc aaaacgaaaa
    14401 acacggccgc gtagccgtat tcgacaaaaa aggcttccaa aagggcaaac atagcggata
    14461 ttccattgtc ggagataaaa agtcagaaca agccgaaaca ttttctgcgc gaaacggcat
    14521 tctatcaaag attatgccgt ctgaaagcgg caaggggcag atttgcgctc ccgccggccg
    14581 ttcagacggc atttaaacag gaaaaccgcc gctgttgaaa cggttttgcc tgattttgcc
    14641 taaaggctgc cgatggcggc ggcaatgcgt tccgcaccgt tgcgcgccca atccgcctgc
    14701 cgcgcctcca ccatcacacg cacgacgggt tcggttcccg aagcgcgcaa cacaaccctt
    14761 cctttgcctt cgagttcttt ttccacttcc gccaacacgt cttttgaggc ttcctgccag
    14821 ttttgccctt tgttgatacg gacattaatc atcgtttgcg ggtacggctg ccagtcggcg
    14881 caaacggtgg cgaggtcttg gttcagcgtt tgcaatgccg ccaaaacttg cagcgcggaa
    14941 ataatgccgt cgccggtgtt gtgtttgtcc atgcacaaaa tatggccgct ggcttcgccg
    15001 ccgatgagcc agccgcgttg gttcagctgt tccaacacat agcggtcgcc gactttggcg
    15061 cggcagaaat cgacgccctg ctctttcagg gcgatttcca tcgccatatt ggtcatgacc
    15121 gtgccgacca cgccgccgat gttgatacct tctcgggcgc gggctttggc aatgacgtaa
    15181 atcaggctgt cgccgtcgta aaccttaccg tttttatcga ccatcatcag gcggtcgccg
    15241 tcgccgtcta aggcgatgcc gtagtcggct tcatgctgta aaacggctgc ctggagtgtc
    15301 ttggtataag tcgcgccgca tttttcattg atattgtagc cgttgggttc gtcgccgatg
    15361 ctgacaactt gtgcgcccag ttcgtgaaac accttgggtg cgacaccgta ccccgcgccg
    15421 ttggcagtat cgataaccaa cttcaaaccc cgaaggtcgg aatggctggg aaaggtagat
    15481 ttgcaaaatt cgatatagcg gtcgtccgca ccactgatgc ggcgtgcgcg accgaggcgt
    15541 gcagaaggct gggttttcat ttcttcgtcg attttggctt cgatttccaa ctcgatttca
    15601 tcgctcagtt tcacgccgcc ctcggcaaag aatttgatgc cgttgtcgga ataggcgttg
    15661 tgcgacgcgg aaatcatcac gccggcggac aggcgcaacg cgcgggtcag ataagccacg
    15721 ccgggcgtag gcagcggacc ggtttggata acgttgacac ctgcggcggt aaaacctgcc
    15781 accaaagcgg cttccagcat ataaccggaa atacgcgtat ccttgccgat taaaacggtc
    15841 ggtttctggt cagtgtcgtg ctgaaccaaa acctgacccg ccgcatagcc gagtttcaat
    15901 acaaaatcgg gcgtaatcgg aaactgcccg acttcgccgc gcacgccgtc cgtgccgaaa
    15961 tatttttttg ccatcacttg ctccgagaat gtgaaccgtt gtccgagatt atacagtcag
    16021 tttgtgcctt gctgtctgca ccgttgatgc cgtctgaagc cgccccgccc ttttcagacg
    16081 gcataaagta tgcgaaccgc cgtttacaga ttgatgccca acgcttccca caccttcaat
    16141 gcatccgccg tcgccttcac atcatgcacc cgcacgattt gcgcgccgcg cgccacggaa
    16201 gccaacgctg ccgccacgct gccgtgtacg cgttccgccg catttgcctc gccggtcagc
    16261 tcgcctatcg tgcttttgcg cgatacgccg atgagcagcg gaaaacctgt ttccgccatc
    16321 aattcgggca aatgccgcat cagcgcgata ttgtgttgca aggttttgcc gaagccggag
    16381 ccgaagccgg ggtcgagtat gatgcgttgc ggtgcgatgc ctgccgcgat acattccgct
    16441 gagcgcgctt tcaaataccg cgctacttca ccgacaacat cttgatattt cggattaatc
    16501 tgcatggttt tgggcaaacc ctgcatgtgc atcaggcaaa tgcccgtgtc cgcctgacgc
    16561 gccagcaatt cgaccgcgcc ctcgtcattc aacgccgcca catcattaat aatatcgacg
    16621 ccgccgagtg ccaacgcttt ttccataatc accgtgcggc gcgtgtccaa actgacggga
    16681 acgccccacc ccgccacttc ctccaataca ggctcaaccc gcgcccattc ttcttcaggc
    16741 gaaacataat ccgcacccag ccgcgtcgat tcgccgccga tgtcgagaat gtccgcaccc
    16801 tctttcaaaa gccgttcggc atgcgccaaa gcggtttggg cgttttgcga atacgcgccg
    16861 ccgtcggaaa aagaatcggg cgtgagattc acgataccca tgattttcgg tttgtccaaa
    16921 ccgatttcaa accgtcctgc ctgccaaacg tgtcgtgcca tctgaactcc tcccaaaata
    16981 aaaaacagat tatatgccgt ctgaaaccgt cttgtgcgct tcagacgaca ccgctattcg
    17041 ggcggcagac ggcatgttgt ccgaatgtct gctccgcctt tgaatctgcc ggtatgcctg
    17101 ctatccgccc gacttttcaa aacaggttcc gacgattccg cacgcgcctg ccgcctttgc
    17161 caagccgtac aggatttcct gcggcatatc gcggttccat aatcccgtaa tattcgcaat
    17221 cacgggcaga tggctgattt ggcggacttt cacgatggat tcgacatcca aacggtaggg
    17281 atggtctttg gtatggctca acacgcccga ctcgcccaga atcaggtggc ggttgccgcg
    17341 cgagacaaca tattctgcgg cattcaacca atcttcggca gaatggtgct tgtctttaca
    17401 cagcaccaag ggaatgttca ggcttccggc ttcattcaat acggcaagat cggacatcag
    17461 cccgccgccc aaatacagga tgtccgcccc cgcattcaaa gccgcttcga catggcggac
    17521 gttgcggacg cgcaccaata cgggtttccc tgcgccatgc gccgatgcgg tctgttccgc
    17581 caaccgtctg caccgtcccc gcccttcatc cgcacttgaa gtgtcgtata agtttgccga
    17641 agtgaaaaac ggatctagaa acactgcatc cgcattgcgc catactgacg gttctgcggc
    17701 gatacagacg gtttccccgc cgccgaaagc cacgcctttg gcggcaacgc ggctgtcttc
    17761 cgcccgattt tcccgactga cggttttcca tgtatccaaa atgcggacgg ctttctcgac
    17821 ctccggcagc gtctgcacct ccctgacgct caaaacccta tcgtcgccga ttgcgccgat
    17881 gacagtacgc tcgtcgccgt gagaaatgtg ttctcgcaga cctctgctgc ggataaaggc
    17941 gacaacgccg gcaatgtccg cttcggcggc acgcctgctc atgacaataa tcatatttcc
    18001 tcctgacaca agaaacggcc tgcccaaaat aggatttttg caagccgtgt tatactgtgg
    18061 cgtgttttac agattgttcg ggctatggat ttattatcgg ttttccacaa ataccgtctg
    18121 aaatatgcgg tggccgtgct gacgatactg cttttggcgg cagtcgggct gcacgcttcc
    18181 gtatatcgca ccttcacgcc tgaaaacatc cgcagccgcc tccaacaaag cattgcacgc
    18241 acacaccgga aaatctcgtt tgatgcggac attcagcgca ggctcctgcc ccggccgacc
    18301 gtcatcctga aaaacctgac cattaccgaa cccggcggcg accagactgc cgtttccgtc
    18361 caagaaacca aaatcggatt gagctggaaa aacctgtggt cggatcagat acagattgaa
    18421 aaatgggtgg tttcgagtgc ggaacttgcc ctgacgcgcg acgggaaagg tgtttggaac
    18481 atccaagacc tgatcgacag ccaaaaacgc caagcctcag tcaaccgcat tatcgtcgaa
    18541 aacagcaccg tccgcctcaa tttcctgcag gaacagctta tcctgaagga aatcaacctc
    18601 aacctgcaat cccccgattc gtcggggcag ccgtttgaaa gttcgggcat actggtttgg
    18661 ggaaagctgt ccgtcccgtg gaaaagcagg gggctgttcc tttcaaacgg catcggcccg
    18721 cccgaaatct caccgttcca ttttgaagct tccacttcgc tggacggaca cggcattacc
    18781 atttccacca ccggcagccc ttctgtccgc ttcaacgccg gcggagcgga tgccgccggc
    18841 ctcggcctgc gtgcagacac ttccttccgt aacctccacc tgaccgccca aatccctacg
    18901 ctggcactca ggaacaacag cattaaaatt gaaaccgtca acggcgcatt taccgccggc
    18961 ggcgaatatg cccgatggga cggttcgttc aaactcgaca aagccaacct gcactccggc
    19021 atcgccaaca tcggcaacgc cgaaatctcc ggcagcttca aaacaccgcg ccaccagacc
    19081 aacttctccc tcaattcgcc gctcgtatgg acggaaaaca aagggctgga cgcgccgcgc
    19141 ctgtatgtat cgacccttca ggataccgtc aaccgcctgc cgcaaccccg tttcatcagc
    19201 cggctcgacg gttcgctgtc cgtaccgaat ctgcaaaatt ggaatgccga attaaacggc
    19261 acattcgacc gccaaaccgt tgccgcgaaa ttcagataca cacatgaaga cgcaccgcat
    19321 ctggaagccg ccgtcgcact gcaaaaattg aacctgaccc cctatcttga cgacgtgcgg
    19381 caacaaaacg gcaaaatatt tcccgacacc ctcgccaagc tgtccggcga catcgaggcg
    19441 cacctgaaaa tcggaaaagt ccaacttccc ggcctgcaac tggacgatat ggaaacctac
    19501 ctccacgccg acaaagacca tatcgcgctc agccgtttca agtcagggct ttacggcggc
    19561 cataccgaag gcggcatcag catcgccaac acccgtcccg ccacttaccg cctgcaacag
    19621 aatgcaagca acatccaaat ccaaccgctg ctgcaagacc tgttcggctt ccacagcttc
    19681 agcggcaacg gcgacgcggt catcgatctg accgcgggcg gcgaaacccg aaaagagctt
    19741 atccgctcgc ttcaaggcag cctgtcgcta aatatttcca acggtgcgtg gcacggtatc
    19801 gacatggaca atatcctgaa aaacggcatt tcgggcaaaa ctgccgacaa tgccgcaccc
    19861 agcacaccct tccaccgatt cacgctcaac agcgaaattt cagacggcat cagccgccac
    19921 atcgataccg aactcttctc cgacagcctc tatgttacca gcaacggcta taccaatctg
    19981 gatacgcagg aattgtctga agatgtcctt atccgcaacg ccgtccatcc gaaaaacaaa
    20041 ccgattcccc tgaaaatcac cggtacggtg gacaaaccgt ccattaccgt cgattacggc
    20101 aggctgaccg gcggcatcaa ttcgcgcaaa gagaaacaga aaatcctcga agacaccctg
    20161 ctggaacaat ggcagtggct caaacctaaa gaaccgtaaa catcctgcgt acacaaatgc
    20221 cgtctgaaac gcccccacgc ttcagacggc agaccgtaaa acctacaacc ccaattcctc
    20281 ccaaatccca tcaatcttag ccgtaaccgc agggtctttt ttgatgacgc tcccccattc
    20341 gcggtcggtt tcgcccagcc atttgttggt cgcgtccaaa cccattttgc cgccgagtcc
    20401 gctgacgggg ctggcgaagt cgagatagtc gatgggcgtg ttttctacca aaacagtgtc
    20461 gcgcacaggg tccatgcgcg tggttactgc ccagatgact tctttccagt cgcgcacgtc
    20521 cacatcgtca tccaccacga tgatgaattt ggtgtacata aactggcgca ggaacgacca
    20581 gcagcccatc atcacgcgct tggcgtgtcc ggcgtactgt tttttcatgc cgatcaccgc
    20641 catgcggtag gaacagcctt cgggcggcag gtagaaatcg gtgatttcgg ggaactgctt
    20701 ttgcaaaagc ggtacgaaca cttcgttcaa tgccacaccc aaaacggcag gctcatcggg
    20761 cggcttgccc gtgtaggtgg aatggtaaat cgggttttcg cgcatggtga tgcgttcgac
    20821 cgtaaacaca gggaaataat cctgctcgtt gtaatagccg gtgtggtcgc cgtacgggcc
    20881 ttccaacgcg gtttcgttcg gatggatgac gccttccaac acgatttctg cgcgggcagg
    20941 cacttgcaaa tcgttgccga tacatttcac cagctccgtc cgcgaaccgc gcagcagtcc
    21001 ggcaaactgg tattcgctca aggtatcggg aacaggcgtt accgcgccca aaatggtggc
    21061 ggggtcgcag ccgagtacga cggcgacggg atacggcgta tcgggattga gtttgcgaaa
    21121 ctcctgataa tccaacgcgc cgccgcgatg cgacagccaa cgcataatca gcttgttttt
    21181 gccgatgagt tgttggcggt aaatgccgag attttggcgt tttttgtgcg gcccgcgcgt
    21241 gacggtcaag ccccacgtta ccagcggcgc aacgtcttcc ggccagcaat gctgaatcgg
    21301 aagttgatac aaatcaacgt cttcgccttc ccacacgatt tcctgacacg gcgcgttttt
    21361 caccacgttc ggcgccatgc tccaaatgtc tttcaagagc ggcagtttgg aaaacgcgtc
    21421 tttgatgcct ttgggcggtt cgggttcttt caaatacgcc agcgtctgcc cgatttcgcg
    21481 cagcttggac acgctgtccg cgcccatgcc catcgccaca cgttcgggcg tgccgaacag
    21541 gtttgccaac acgggataat catagcgcgt accgtcgggc ttaatcgggt tttcaaacag
    21601 caacgccggc ccttcggcac gcagcacgcg gtcggcgatt tcggtcattt ccaaatgcgg
    21661 ggaaacgggg tgtgcgacgc gcttgagttt gccctgctgc tcgagcatgg cgatgaagtc
    21721 gcgcaggtct ttatatttca tgttgatcct ttttgtcctt ttatcctgaa caatccgatt
    21781 tggataccgc ccctatcctt gcctgcgctt cggcatattc tatgccgtga taaaagtcgc
    21841 gtaccagcgg atgttcgccg ccttgatgga gttgcaacaa aggacgttga ccatcgggtt
    21901 gggtaacgac attgcagtgc agaccgaagg tgtcggattc gtaagggggc agccggttgc
    21961 agatcatgcc gaaataaacg gcgttttcag ggttgtcgta aaagcggctt tgatagtcgt
    22021 taaaactctt ttcgctgacg gatacccaca cgccatattc cagcgtttcc tcatgcccga
    22081 taatcggaat cggcagcacc gcgcggataa agcggtcggt ttggtcggga tagcggatga
    22141 tgcagaaatc agaatcgcat tctgcttggt aagcaatgcg ttcttcttca ctgagttgat
    22201 tatagggata gggggcggta aagccgattg cgggcatatc ttcatgtttt tcgccgcagg
    22261 aagtgcaagt gtacataagg tttcagactt tcaaaacgag tttgcggtaa agccattcgc
    22321 cggcaaacgg catccccatc aatacatagg caatcacgcc ggtataaacc gcccaccaat
    22381 catatccccc aaccgtgcca acaaagcggc aagcgtcccg ttggcaataa agaatacgca
    22441 ccaaacctgc gttacccggc gggtatagcg cacagctttt tcaggcaggt cgggctgttg
    22501 cagccgcgcg agcctttcta tgaccgtctg cccggcaaac aggctgccgc cgaacaccgc
    22561 caacatcatc agattgacga ggacgggata ccaatacatc gaatcatgcc gcccgaacac
    22621 caatactgcg gcaaaaaaca gtgtaataaa caaagccgca taacgctgtt ggggcagttt
    22681 ggcagtcagg gcgcgcagca gccacaaccc gcacatcgcc gccgccagcc agacaaacca
    22741 gcccgcctcc ctgccgtaat atagtggatt aacaaaaacc agtacagcgt tgcctcgcct
    22801 tgccgtacta tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataccaca
    22861 aagcgggata ggcaatgctt aatacggtca gaaaaatatg tccgaaaaaa ccgggtttca
    22921 ttttgaatcc gcacaaatgt tttcagacgg catccgataa aaacatgccg tctgaaaaat
    22981 aattagaaat acccgattag cccgcctgaa tcttcaatac cgcctgtacc acgtcgttga
    23041 cggtgcggac attgcggaaa tcttcagcct gcagcttgcg ggctgccaca accattcacg
    23101 attgcgatat acccggcggc attcccgact gccgttcagc agaaagcgca cccattccaa
    23161 accgctccaa taggcctcgg gcagcatacc ggcttcagac ggcatatcgt ccgaagcaga
    23221 taaagtcagg ctgtaacgcg tccctttggt cagaaccatc gccaaagcat aagcaaacgg
    23281 cgcgcgcgtt gccgatacga catatccttc cggcagcgga tcgtccgccg ccaaaaccaa
    23341 aaccgacccg catccctctt ccaacagtga tgccgcttcc gccaatgccg tttccacacc
    23401 gtcggcacac acggccaatg ccgtctgctc gctcatatcc cgcctcaata tcgaccatta
    23461 ccccgccgtt gcattgtgca ccgacaaacc gaacgaagtc ggcgacacga tttgcgattt
    23521 caacagttcc aaccacaaat cgaaactgcg cgccatttcc ccgtcgtgcg aggcataaac
    23581 tatagtggat taaattttag gggctgtact agattagcag atatgttacc ctcgaaatat
    23641 gaagataaca cactgcaaat taaagaaaaa agtacagaaa gaactgctcc gttttttgt
//
LOCUS       DAWKQB010000015        22031 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.015, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000015 DAWKQB010000000
VERSION     DAWKQB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.015"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(242..563)
                     /locus_tag="WJL14_000454"
                     /pseudo
     CDS             complement(242..563)
                     /locus_tag="WJL14_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(550..888)
                     /locus_tag="WJL14_000455"
     CDS             complement(550..888)
                     /locus_tag="WJL14_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7655576.1"
                     /translation="MTGFNDAAGVASSPSDIKGKYVQKVEVAKGVITAQMASSNVNNE
                     IKGKKLSLWAKRQNGSVKWFCGQPVQRDKADADADAVTADTAANKKIDTNHSHEPTSR
                     HSRESGNDGI"
     gene            complement(949..1290)
                     /locus_tag="WJL14_000456"
     CDS             complement(949..1290)
                     /locus_tag="WJL14_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7655577.1"
                     /translation="MASSNVNNEIKDKKLSLWAKRQDGSVKWFCGQPVTRTAADSDDV
                     AAANGKTADNINTKHLPSTCRDASSAVCTKTPRADFKHFQKISRYRVLPESRQMAEKL
                     RHSRKSGNLVR"
     gene            complement(1319..1648)
                     /locus_tag="WJL14_000457"
     CDS             complement(1319..1648)
                     /locus_tag="WJL14_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7655578.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(1726..1944)
                     /locus_tag="WJL14_000458"
     CDS             complement(1726..1944)
                     /locus_tag="WJL14_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ7655579.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            2066..3019
                     /locus_tag="WJL14_000459"
     CDS             2066..3019
                     /locus_tag="WJL14_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ7655580.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALIAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3095..5152)
                     /gene="metG"
                     /locus_tag="WJL14_000460"
     CDS             complement(3095..5152)
                     /gene="metG"
                     /locus_tag="WJL14_000460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ7655581.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGNECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFGIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAHDQYGDNCEVCG
                     TTYSPTELINPYSAVSGVKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGCLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPKVAEN
                     AAKFLNLEAITWANTRETLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATSDGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(5268..7106)
                     /gene="glmS"
                     /locus_tag="WJL14_000461"
     CDS             complement(5268..7106)
                     /gene="glmS"
                     /locus_tag="WJL14_000461"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ7655582.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGSIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGKLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLAAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVVAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARDTDVDKPRNLAKSVTVE"
     gene            7389..7910
                     /locus_tag="WJL14_000462"
     CDS             7389..7910
                     /locus_tag="WJL14_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655583.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVVTGEEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTVQAYLDAQKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGRPRALLL"
     gene            complement(8189..9514)
                     /locus_tag="WJL14_000463"
     CDS             complement(8189..9514)
                     /locus_tag="WJL14_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ7655584.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELAGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTSKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGETAGKMKEPGYVWQLLPNGMKPEYRP"
     gene            9725..10564
                     /locus_tag="WJL14_000464"
     CDS             9725..10564
                     /locus_tag="WJL14_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ7655585.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKRQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVVGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIAVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            10561..11727
                     /locus_tag="WJL14_000465"
     CDS             10561..11727
                     /locus_tag="WJL14_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ7655586.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGETQSANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDTGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            11991..13256
                     /gene="efeB"
                     /locus_tag="WJL14_000466"
     CDS             11991..13256
                     /gene="efeB"
                     /locus_tag="WJL14_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ7655587.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(13328..13657)
                     /locus_tag="WJL14_000467"
     CDS             complement(13328..13657)
                     /locus_tag="WJL14_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ7655588.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(13734..15104)
                     /gene="glmU"
                     /locus_tag="WJL14_000468"
     CDS             complement(13734..15104)
                     /gene="glmU"
                     /locus_tag="WJL14_000468"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ7655589.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHEIGGETMLGRVIDTAA
                     ALNPQNICVVVGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDASAA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVRVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNQIGPYARLRPQ
                     AKLANDVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDGKLALARSRQTIIDGWVR
                     PEKNKQ"
     gene            complement(15159..15431)
                     /locus_tag="WJL14_000469"
     CDS             complement(15159..15431)
                     /locus_tag="WJL14_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ7655590.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(15407..16138)
                     /locus_tag="WJL14_000470"
     CDS             complement(15407..16138)
                     /locus_tag="WJL14_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ7655591.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMADTENTLCR
                     LNGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTPPRKYERMLCVKPSLS"
     gene            16197..17198
                     /locus_tag="WJL14_000471"
     CDS             16197..17198
                     /locus_tag="WJL14_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7655592.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVAINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            17292..18140
                     /locus_tag="WJL14_000472"
     CDS             17292..18140
                     /locus_tag="WJL14_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7655593.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAEVIVGVDYDCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            18166..18651
                     /locus_tag="WJL14_000473"
     CDS             18166..18651
                     /locus_tag="WJL14_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ7655594.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(18753..20321)
                     /gene="msrAB"
                     /locus_tag="WJL14_000474"
     CDS             complement(18753..20321)
                     /gene="msrAB"
                     /locus_tag="WJL14_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ7655595.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSFNMRRIEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            20466..21731
                     /gene="ftsY"
                     /locus_tag="WJL14_000475"
     CDS             20466..21731
                     /gene="ftsY"
                     /locus_tag="WJL14_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ7655596.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4676 a         5487 c         6565 g         5303 t
ORIGIN      
        1 tccgcttgtt cggtacatga gagggcggat tgcgcgcctg tcagataaac cccgtgttta
       61 aacgggcggc aatgaggtac gcgcagagcc ttgaagcgca atcgatatat tattttcagc
      121 caaaacggac gcccccgctt gccttgcaaa cccttaaaaa ggaagccacc cggattaatc
      181 cgagtggccg tggaaaatca cttaccgctt gatttattta aaatttatgg tataatttgc
      241 cttatccggt agatgaatca tcgcggcagg ttgacggcag gtgcttggtg ttgattttgt
      301 cgtcggtgcc gttggcggtg gcggctttga cgtcgtcttt ggctttgtcg gcgcgcgcaa
      361 ccggctgtcc gcagaaccat tttaccgaac cgttttgacg cttggcccac agggagagtt
      421 ttttgccttt gatttcgttg tttacgccgc ttgaaagcat tgtggcggta atgacgccgt
      481 ttgcgacttt aacttcctta acatatttgc ctttgatgtc ggtgggggga tgccacgccg
      541 gcggcatcat taaatcccgt cattcccact ttcgcgggaa tgacgggatg taggttcgtg
      601 ggaatgattg gtgtcgatct tcttgttggc ggcggtgtcg gcggtaacgg cgtcggcgtc
      661 ggcgtcggct ttgtcgcgct gaaccggctg tccgcagaac cattttaccg aaccgttttg
      721 acgcttggcc cacagggaga gttttttgcc tttgatttca ttgtttacgt tgcttgaagc
      781 catttgggcg gtaatgacgc cttttgcgac ttcaactttc tgaacatatt tgcctttgat
      841 gtcggagggg gaggatgcca cgccggcggc atcattaaat cccgtcattc ccactttcgt
      901 gggaatgacg ggattaaagt ttcaaaattt attctaaata actgaaactc aacgaactag
      961 attcccgctt ttgcgggaat gacgaagttt ttctgccatt tgccgtgatt cgggcaatac
     1021 tcggtaacgg ctgatttttt gaaagtgttt gaaatcggcg cgtggtgttt ttgtgcaaac
     1081 ggcagatgac gcatcgcggc aggttgacgg caggtgcttg gtgttgatgt tgtcggcggt
     1141 cttgccgttg gcggcggcga cgtcgtcgct gtcggcggcg gtgcgcgtaa ccggctgtcc
     1201 gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtt ttttgtcttt
     1261 gatttcgttg tttacgttgc ttgaagccat tatgtcagac ggtattgccc gggcagcttt
     1321 attcgtacac tttcagcagc tcgacttcaa atatcaaagt ggcgtgcggg ggaatcacgc
     1381 cgcccgcgcc gtgtgcgccg tagcccattt ccgaagggat ggtcagcttg cgtttgccgc
     1441 cttccttcat tccgccgaag ccttcgtccc agcctttgat gacttgtccg acgccgagcg
     1501 tgatggtcag cggctggcgg cggtcgaggc tggagtcgaa tttggttccg ttttccagcc
     1561 aacctgtgta atgcacggta atctctttgc ctttaactgc ttcttttccg aagccttcct
     1621 gcaagtcttc aataatcagg ccgcccatat ttgtcctttc gttgcttgtt ggtcaaaacg
     1681 gcaagggtaa cataccgtcc ggcgaagtca aatgccgctc aaacgtcagc tgcatcggtg
     1741 cagctgaaac ggctgtgttt gttggactgt tttatttttt tcgtaaaggt tccatgcttt
     1801 ttcatggaaa tagaaaacga cggtgttgat taggggttcg accagcgcaa ctgctcccga
     1861 tacgcctata ctgcccgtca gtacataggt tacactgaag gcgacgctga aatgcagtgc
     1921 ggcaaaagtc agggttttaa gcatcatcct ctcccggatt ggacattgac ggagagatga
     1981 taaagattat cataaggctg cgcggtttaa atttgctatt tgttgttagt gtagataaat
     2041 cgttttttaa ataaggatag gaattatgaa tcataaaaag atagtcgtat tggatgcgga
     2101 tactttgccc ggccgggttt ttcattttga ttttccgcac gagcttgcgg tttacggtac
     2161 gacaggtgcg gatgaaacgg cagaacgggt gcgcgacgca catattgtca ttaccaataa
     2221 ggtaatgatt tccgccgaca ttattgcggc taatccgcag ttggagctga ttgccgtcag
     2281 tgcgaccggt gtgaacaatg tcgatattgg ggcggcaaag gcggcgggcg ttgcggtatg
     2341 caatgtccgc gcatacggaa acgaatcggt tgcggaacac gcctttatgc tgatgattgc
     2401 cttgatgcgg aatttgcccg cctatcagcg tgatgttgcg gcaggattat gggagaagtc
     2461 gccatttttc tgtcattacg gcgcgccgat tcgggatttg aacggcaaaa cgctggcggt
     2521 tttcggacgc ggcaatatcg ggaggacgct ggccggatac gcgcaggcat tcggtatggg
     2581 ggtggtgttt gccgaacaca aacacgcgcc cgacgtgcgc gaaggctatg tttcctttga
     2641 agatgcggta cggactgccg acgtgttgtc gctgcactgt ccgctgaacg cccaaactga
     2701 aaatatgata ggcgaaaacg aattacagca gatgaagccc ggtgcggttt tgattaattg
     2761 cggacgcggc gggctggtgg atgaaaacgc gctgattgcc gcactcaaat acgggcagat
     2821 cggcggagct ggtgtcgatg ttttgacgga ggagccgcca aggggcggca atcccttgct
     2881 gaatgcacgc ctgcccaatc tgattgttac gccgcatacc gcgtgggcaa gccgcgaggc
     2941 gttggacagg ctgtttgata tattgctggc gaacattcac gcctttgtga aaggcgaggc
     3001 gcaaaacagg gtggtttgag cttgtcggga tgacgaaaaa caatgccgtc tgaacgcctc
     3061 aaggattcag acggcattgt tttgatattt ctgcttaacc gactttgtcg cccggttgcg
     3121 cgcctgcgtc gacatccaag agcttcagtt tcccatctga cgtggcggca ctcaaaatca
     3181 tgccttcgga tacaccgaat tttgccattt tgcgcggggc gaagttggcg acggcgatga
     3241 ccatccttcc gtttaactcg gcagggttcg ggtaagacgc ggcgatgccg gagaagatga
     3301 tgcgtttttc aaaaccgaaa tcgaggtcga atttcaaaag tttggtgctg ccttcgacgg
     3361 cttcgcagtt caatactttg gcgacgcgca tatcgatttt cataaagtcg tcgaagctcg
     3421 cctgttcggc gactttttcg tatttgctct cttcggcggc aggcgctgct gctgctgcga
     3481 tactttgttt gttggcttcg atcaaatcat ccacttgttt ttgctccact cgttgcatta
     3541 aatgttcgta tttgttgatg gcgtgtttgc ccaaggtttc gcgtgtattt gcccaagtga
     3601 tggcttccaa attcaggaat ttggcggcat tttcggccac tttcggcaac acgggggcga
     3661 ggtaggcggt cagcatggta aaggcgttga tgagttcgct gcatacttcg tgcaggcgtt
     3721 cgtcttggcc ttcttgtttg gcgagttccc aaggtttgtt ggcatcaacg tattcgttga
     3781 caatgtctgc caaggccatg atgtcgcgca gggctttggc gtattcgcgg ctttcgtagc
     3841 attcggcaat ggcttcgctt tgtgcggtca gttttgccag caatgcgctg tcggcaacgt
     3901 ctttcagaca gccttcaaag cgtttggcga tgaaacctga ggcgcgggcg gcgatgttga
     3961 cgtatttgcc gacgaggtcg ctgttgacgc ggctgataaa gtcttgcagg ttcaaatcga
     4021 tgtcttcgat tttgctgttg agtttggcgg cgatgtagta gcgcatccac tcggggttta
     4081 aaccttgttc cagataggat ttggcggtga taaacgtgcc gcgcgatttg gacatttttt
     4141 gtccgtcgac ggttaggaag ccgtgtgcgt acacgccggt cggggcgcgg tggccggaga
     4201 aatgcagcat ggcaggccag aacagggcgt ggaaatagag gatgtctttg ccgatgaagt
     4261 ggtacatctc ggtttggctg tcggctttga agtattcgtc aaaatcgacg ccgatgcggt
     4321 cgcacaggtt tttaaacgac gccatgtagc cgacgggcgc gtccagccag acgtagaagt
     4381 atttgcccgg cgcatcgggg atttcaaaac cgaaatacgg cgcgtcgcgg gaaatgtccc
     4441 agtcggacag tgtggtttct tcgccttcgc ccagccattc tttcattttg ttgagggctt
     4501 ctggttgcag atggggcttg ccgtcgtgcg ggttgttgcc ggaagtccaa gctttgagga
     4561 agtcggcgca ttcgcccagt ttgaagaaga agtgttcgga ttcgcgcaat tcgggtttca
     4621 cgccggaaac ggcggaatac gggttgatca gttcggtcgg ggaataggtc gtgccgcaga
     4681 cttcgcagtt gtcgccgtat tggtcgtggg cgtggcattt ggggcattca cctttgacga
     4741 aacggtcggg caggaacatt tgtttttcag ggtcgaaaag ctgctcgatg acgcggcttt
     4801 cgattttgcc gttggctttc agcgcgcggt aaatgtcttg ggaaaactgt ttgttttcag
     4861 gggaatgggt gctgtaataa ttgtcgtagc cgatgccgaa accggtaaag tcggcgaggt
     4921 gctcttcgcg cactttggca atcatgtctt cgggggcgat accttgtttt tgcgcggcaa
     4981 gcatcacggg cgtgccgtgg gtgtcgtcgg cgcagcagta gtggcattcg ttgccgcgca
     5041 gtttttggaa gcgtacccaa acgtcggttt ggatgtgttc gaccatgtgg ccgaggtgga
     5101 tgctgccgtt ggcatagggc agggcggagg taacgaggat tttgcgtgtc atattgtgct
     5161 ttgcaaacaa agggtaaagg cggattatac cgcaaatcaa acggggaaat gccgtctgaa
     5221 gcctgaaaaa tcaggcttca gacggcatgt tcgcaaacaa tccggactta ttcgaccgta
     5281 acggatttcg ccaggttgcg cggtttgtct acatcggtgt cgcgtgcgag ggcgacatgg
     5341 taggaaagga gctgcacggg gatagtatgc acgacggggg acagtttgcc gacgtggcgc
     5401 ggtgcgcgga taacgtgcac accttcggtg gcattaaaat tgctgtcgag gtcggcaaag
     5461 acgaaaagtt cgccgccgcg cgcgccgact tcctgcatat tggctttgac tttgtccaac
     5521 aggctgtcgt tgggtgcgac gacgacgacg ggcatgtttt cgtccaccaa ggcaagcggc
     5581 ccgtgcttca gttcgccggc aggataggct tctgcgtgga tgtaggtgat ttccttcagt
     5641 tttaacgcac cttcgagggc aatcgggtaa tggatgccgc gccccaaaaa cagcgcgctg
     5701 gttttcttgg caaactgttg cgcccaggcg gcaatttgcg gttcgaggtt taaagcgtgc
     5761 tgcacgctgc cgggaagctg gcggagttct tcggtgtaac gtgcttcgtc ttcttcggaa
     5821 accaaaccgc gcactttcgc cagcgttacc gccaaaccga acagcgcaac cagctgcgtg
     5881 gtaaatgctt tggttgaggc gacgccgatt tccgcaccgg cacgggtata aagcacgagg
     5941 ctgctttcgc gcggcagggc ggattccatc acgttgcaga tggagaggct gtggcggtgt
     6001 cccaaggatt ttgcgtattt caacgcctcc atcgtgtcca gcgtttcgcc ggattgggaa
     6061 atggtaatga ccagttgatc cggatcggca atcacgctgc ggtatcggta ttcgctggcg
     6121 atttcgacat cggctgggat tttggcgatg gattcgagcc agtattttgc ggtcagcgcg
     6181 gcgtaatagg acgtgccgca ggcaaggatt ttgacgctgc ggatgctttc aaacacgctt
     6241 ttggcgtttt tgccgaagtt ttcggggatg aagccgccgt cgaggaaaac ttccgccgta
     6301 tccgcaatcg cgcgcggctg ctcgtggatt tccttttgca taaagtggct gtacggtccc
     6361 agttccaaag aggcgagcga gagttcggat accttgactt tgcgttcggc ggccaggccg
     6421 tttttatcgg tcagccgttt gatgccgtct gaagccagca gcgcgatgtc gccgtcttca
     6481 aggtacgcca cgcggcgcgt aaaggcgatg acggcggata cgtccgaagc gataaaggtt
     6541 tcatcgtcgc ccaaagcgac taaaagcggg cagcccatac gcgccacaac taattcatca
     6601 ggcttgtctt gggcaataac cgcgatggcg tatgcgccgt ggaaacgttt gaccgcttcc
     6661 tgcactgctt caaacaattt gccgccgttt tgcgcgtatt cgtgattgat gctgtgggcg
     6721 atgacttcgg tatccgtttg cgattcaaaa cggtatccca aaccttccaa gcgtttgcgt
     6781 tcgctttcaa agttttcgat gatgccgttg tggacgaccg caatcatacc gccgctgatg
     6841 tgcgggtggg cgttcggctc ggtaacgccg ccgtgtgtcg cccaacgcgt atgcccgatg
     6901 ccgatgctgc cgctgatgcc tttttcgcgt gctgcgtcct ccataagctg cacgcgtccg
     6961 acgcggcgca cgcgtttgat tttgccgtcg gtgttgacgg caatgcctga tgagtcataa
     7021 ccccggtatt cgaggcgttt gagaccgtcg gtcagaaaat cgacgacgtt gtgatgggcg
     7081 cggatggcgc cgacgatacc gcacataact gttccttagt atccggttga aaaaaaaaca
     7141 ggcgcggacg gcttccgtgc cgcaccttcc tcttcggatt ataaaccgcc ttccgcgccg
     7201 aaaaacagca aaatgccgtc tgaaggcttg ggcttgctca aaaaagggag ggatttcccc
     7261 gcctgactgg gatgggcgtt cagacggcat tgcctgctgc tggtttatag tttttgcaaa
     7321 tcaatattga caagctgaaa aaaaaaaaca atatactcgc ccagtcttaa tgttaaagga
     7381 gtatggaaat gaaacaaatg cttttggccg tcggcgtggc ggcggtgttg gcgggctgcg
     7441 gcaaggatgc cggcggttac gagggttatt ggcgcgaaaa atcggacaaa aaagagggta
     7501 tgattgccgt caaaaaagaa aaaggcaatt acttccttaa taaaatcaac gtggttacag
     7561 gcgaggaaga gtctttgctt ttgtctgaaa aagacggcgc gctttcgata aacacaggga
     7621 taggggaaat cccgatcaaa ctttccgacg acgggaaaga gctgtatgtc gaacgcaggc
     7681 agtatgtcaa aaccgatgcg gcgatgaagg acaaaatcat cgcccaccag aaaaagtgcg
     7741 gacaaacagt acaggcatac ctcgacgcgc aaaaggcgtt gccgtcaaac caaacgtatc
     7801 agcagcgtca ggcggcgatc gagcaattga aacggcggtt tgaagccgag ttcgatgaat
     7861 tggaaaaaga aatcaaatgc aacggcagac ctcgggcatt gttgctttag cggaggataa
     7921 ccgggaggat gccgccgccg aatcggatgt gcggtttcct gactggtacg ggcgggcggg
     7981 aatgtccgcc tttttttgtt cggacgcgtt tgaatacccg tccgattccg atcgtttgca
     8041 agggggtatt tccgttcggg cggaaatcca ttccgtcaaa taaaaaaagg cattttgtcc
     8101 agccggcttg tgcctgcggt atctcaaaac ttcgccttaa tgcgcccgat tggccgtgat
     8161 gggcttcaga cggcattgtt ttgccggttt acggccgata ttcgggcttc atgccgttgg
     8221 gtagaagctg ccagacgtaa cccggttctt tcattttacc agccgtttcc cctgcttcgt
     8281 cgccgtaacc ccagaaataa tccacgcgca ccgcgccttt aatcgcgctg ccggtatcct
     8341 gcgccataat caggcggttg agggctttgc tggtaactgg atgggcggtg gcgacaaata
     8401 agggcgcgcc caaggtaatg tagtgccggt cgactgcgcc ggcatactcg cccatcagcg
     8461 gcgtgcccag tgcgccgacg gggccgtcat tgctgcttcc ggcaagctct cggaaaaaga
     8521 tatagctggg gttttgaccc aaaacttcgg cgaggcgttg cggattttgc cgcatataag
     8581 acttaatgcc ctgcatggag gtttgtccga gtttgaggta gcccttatcc gccatgtatt
     8641 ttccgatgga aacatatgga tgctcgtttt tgtcggcata gccgatgcgg atgtatttgc
     8701 cggacggggt tttcagacgg cccgagcctt ggatgtgcat aaaaaagagt tcgacggggt
     8761 cttcggcgta accgagtatc ggtgctttgc cgtcaagcgc gccgccgttg atttggtttc
     8821 gcgtgtggta ggggaggaag cggcttcctt caaacctgcc cttgattgcc gttgtgcgtg
     8881 cggtgatggg gaatcgggag aggtcggcgg tgtgcgtgcc gccggcattg tcgattgtgc
     8941 cgctgttttt ccccgtctgc ctgatgcgga caagggcttt tccgctccgc aaaccggcag
     9001 gcagggggac ggagataaaa tcgtcgggaa taccgtaaat cgggaagcgg gcctgtgccg
     9061 tccgtctgtc gtcgcccttc agtaccggtt cgtaatagcc ggtaaccgtg ccggcaaggc
     9121 ttccgttgcc tgcaacctgc cacggcgtga aatagcgttc aaaaaactgt tttgcctgaa
     9181 aggaatggac gggggtttga aaggcttggg cacacacatc ctgccagcct tggcggtttt
     9241 tcaaattggc gcagccgagg cggaaggatt gcaggctttt ggcgaaatcc tgcgccgccc
     9301 agtggggcag ggacaggtgc ggcacaacgg tataaacggc cccgccgccg ccgaccgtcg
     9361 ttccggcggg gtcggggatg ccgaccggcc ggtccgggcc gttgatgacg gatgtgtcgg
     9421 gttgcggaaa ggtttggatg ctcttgcttt ggcaggcggc gaggatggcg gcggcgatgc
     9481 cgtacagggc ggcgcggaat aggtattttt tcataatgga caatgttgcc ggcagtaata
     9541 agaaagatgg tttcgggcgg cgttgcggca gccgtggaga ggggatttta acacagggcg
     9601 cagctgcagc ctgcggaact ttccgccgcg cggcaatgca gataaaaata acttgcattt
     9661 gtatttacaa acaatgaaaa tattccgata atatattatt catcatcctt gttcgttcgc
     9721 gtttatgctg gtcgcttttt taattatgtt gcgcgagggt atcgaagccg cgctcatcgt
     9781 cggcattgtt gccggttttt tgaaacagtc cggacattcc aaactgatgc ctaaggtctg
     9841 gttcggggtc gtccttgctt ctttgatgtg tttggggctg gggtacggaa tccattcggc
     9901 aacgggcgag attccccaaa agcagcagga gttcgtcgtc ggcattatcg gtttggttgc
     9961 tgtcgcgatg ctgacttata tgattttatg gatgaaaaaa gccgcccgtt cgatgaagcg
    10021 gcagcttcag gactctgtgc aggcggcttt gaaccgtggc agcggtcaag gatgggcctt
    10081 ggttggtatg gcgtttcttg ccgtggcgcg cgaaggtttg gagagcgttt ttttcctgct
    10141 tgctgtattc aaacagagcc cgacgtggca gatgccggcc ggtgcggtag tgggggtttt
    10201 ggctgccgcc gtgattggcg cgttgattta tcagggtggg atgcgcctga atctggcgaa
    10261 gtttttccgt tggacggggg cgtttttgat tgccgttgcc gccggtctgc ttgccggctc
    10321 gctgcgcgcg ctgcatgagg caggtatttg gaacgcgctt caggatattg tgttcgactc
    10381 atcaaaatat ttgcacgaag acagcccgtt gggcgtgctg ctcggcggat ttttcggcta
    10441 taccgaccat ccgacgcagg gcgaggcctt ggtttggctg ctgtatctta ttcccgtcat
    10501 gacttggttt ttgtgcggca gcaggccgtc tgaaacttta acccgtaaag aggagctgaa
    10561 atgagaaaat tcaatttgac cgcattgtcc gtgatgcttg ccttaggttt gactgcgtgc
    10621 cagccgccgg aggcggagaa agctgcgccg gccgcgtccg gtgagaccca atccgccaac
    10681 gagggcggtt cggtcagtat cgccgtcaac gacaatgcct gcgaaccgat ggaactgacc
    10741 gtgccgagcg gacaggttgt gttcaatatt aaaaacaaca gcggccgcaa gctcgaatgg
    10801 gaaatcctga aaggcgtgat ggtggtggac gagcgcgaaa acatcgcccc cggactttcc
    10861 gataaaatga ccgtcaccct gttgccgggc gaatacgaaa tgacttgcgg tcttttgacc
    10921 aatccgcgcg gcaagctggt ggtaaccgac agcggcttta aagacaccgc caacgaagcg
    10981 gatttggaaa aactgtccca accgctcgcc gactataaag cctatgttca aggcgaagtc
    11041 aaagagctgg tggcgaaaac caaaaccttt accgaagccg tcaaagcagg cgacattgaa
    11101 aaggcgaaat ccctgtttgc cgacacccgc gtccattacg aacgcatcga accgattgcc
    11161 gagcttttca gcgaactcga ccccgtcatc gatgcgcgtg aagacgactt caaagacggc
    11221 gcgaaagata ccggatttac cggctttcac cgtatcgaat acgccctttg ggtggaaaaa
    11281 gacgtgtccg gcgtgaagga aattgcagcg aaactgatga ccgatgtcga agccctgcaa
    11341 aaagaaatcg acgcattggc gtttcctccg ggcaaggtgg tcggcggcgc gtccgaactg
    11401 attgaagaag tggcgggcag taaaatcagc ggcgaagaag accggtacag ccacaccgat
    11461 ttgagcgact tccaagccaa tgtggacgga tcgaaaaaaa tcgtcgattt gttccgtccg
    11521 ttgatcgaga ccaaaaacaa agccttgttg gaaaaaaccg ataccaactt caaacaggtc
    11581 aacgaaattc tggcgaaata ccggactaaa gacggttttg aaacctacga caagctgggc
    11641 gaagccgacc gcaaagcgtt acaggcctct attaacgcgc ttgccgaaga ccttgcccaa
    11701 cttcgcggca tactcggctt gaaataagcc gcaagcgttc agacggtatt tggcggcaga
    11761 taccgtctga agttttatag tggattaaca aaaaccagta cggcattgcc tcgccttgcc
    11821 ttgccgtact atttatactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
    11881 ctatatccgc catatattgc agggcgggat ttcaacctgc cgctatcggt taatggaaaa
    11941 acggcgtgca gggataccca tcctgctgca cggatattga aggaaacacc atgagcaaaa
    12001 accaacccgc acaaccgacc aggcgcactc tttttaaaac cgcgatcgca gctggagcag
    12061 tcggcgcaat cggaggttat ctcggcggca aaaaacgggg cgaaaccgcc gaacgcaccg
    12121 ccgaaagcca acactcgccc caagcctatc cctgctacgg cgaacatcag gcaggcatcg
    12181 ttacgccgca gcaggcgttt tcgattatgt gcgccttcga cgtaaccgcg caaagtgcca
    12241 agcagctgga aaacctgttc cgcacgctga ccgcccgcat cgagtttctc acccaaggcg
    12301 gcgaatacca agacggcgac gacaaacttc cgccagccgg cagcggcatt ttgggcaaag
    12361 ccttcaaccc cgacgggttg accgttaccg tgggggtggg cagcagcctg tttgacggcc
    12421 ggtttggact caaagacaaa aaaccgattc atttgcagga aatgcgcgac ttctccaacg
    12481 ataagctgca aaaaagctgg tgcgacggcg atttgagcct gcaaatctgt gccttcaccc
    12541 ccgaaacctg ccaagccgcc ctgcgcgaca tcatcaaaca caccgtccaa accgccgtta
    12601 tccgttggag tatcgacggg tggcagccta aatccgaacc cggcgcgatg gcggcgcgca
    12661 acctgttggg cttcagggac ggcacgggca accccaaagt ttccgacccc aaaactgccg
    12721 acgaggtttt gtggacgggt gtggccgcca acagcctcga cgaaccggag tgggcgaaaa
    12781 acggcagcta tcaggcagtc cgccttatcc gccactttgt cgagttttgg gacaggacgc
    12841 cgcttcaaga gcaaaccgac attttcgggc ggcgcaaata cagcggtgcg ccgatggacg
    12901 gcaaaaaaga agccgaccaa ccggattttg ccaaagaccc cgaggggaat accacgccca
    12961 aagacagcca tatacgcctg gcgaatccgc gcgatcccga attcctcaaa aaacaccgcc
    13021 tcttccgccg cgcctacagc tattcgcgcg gactcgcctc aagcggacag cttgatgtcg
    13081 ggctggtgtt cgtctgctat caggcaaacc ttgccgacgg attcatcttc gtgcaaaacc
    13141 tcctcaacgg cgaaccgctg gaagaataca tcagcccctt cggcggcggc tatttcttcg
    13201 tcttgcccgg cgtggaaaaa ggcggctttt tggggcaagg gctgctgggc gtataaatcc
    13261 gccatataaa aaacgccgtc cgaaccttgc caaacgggtt cggacggcgt ttcttgtttt
    13321 tgggcggtca ggcttttttg acgaattcgg attttaaatt catcgcgctg ccgtcgattt
    13381 tacagccgat gttgtggtcg ccttcctgca ggcgtatgcc tttgactttt gtaccttgtt
    13441 tgatcaccat cgagctgcct tttaccttga ggtctttgat gaggatgacg gtatcgccgt
    13501 tttgcagcac tgcgccgttg gcatcgagca cttgagccgc aaggtcggcg gcggattcgg
    13561 tttcattcca ttcgtgggcg cattcggggc agatgtattg tccgccgtct tcataagtgt
    13621 attcggaggc gcattgtggg catgggggta atgacatggt ttgcagtcct tatctgatat
    13681 cggaggagtt tgatgccgtc tgaaacctga aaccggcttc agacggcata gctttattgt
    13741 ttgttttttt caggcctcac ccagccgtcg atgatggttt ggcgggagcg ggcgagggcg
    13801 agtttgccgt cttcgacatt gcgggtaatc gtgctgcccg cgccggtggt tactttgttg
    13861 ccgattttga cgggggagac caagacgcag tttgaaccga tgcgcacttc gtcgccgatg
    13921 acggttttgt gtttgtgcac gccgtcgtag ttggcaataa tcgtacctgc gccgaagttg
    13981 gttttgctgc cgacttcggc atcgccgatg taggtgaggt ggttggcttt ggtgcctttg
    14041 ccgatggcgg cgtttttgat ttcgacgaag ttgccgacgt gtacgtcgtt agctagtttg
    14101 gcttgcggac gcaaacgggc gtatgggccg atttggttgt tttcgccgac ttcgcagtct
    14161 tcgaggtggg agaagggggc gattttgctg tttgcgccga ttttggcgtt tttgatgacg
    14221 cagtttgcgc cgatttcgac gttgtcgccg atttcgactt cgccttcaaa gatacagttc
    14281 acatcaatta cgacatcctg tccgtgtttc agacggcctc gtaaatcgaa acgtgccgga
    14341 tcgcgcaggg ttacgcctgc tttgagcaat tcttgtgcct gttcggtttg gaagatgcgt
    14401 tcgagttcgg cgagctggcg tttgttgttc acgccggcgg cgaggtggga ggcgcgcact
    14461 cggacgggat gaactttaat accgtcggca acggctttgg cgatgaggtc ggttaaataa
    14521 tattcgcctt gggcattgtt gctggaaagg ctgttcagcc agttttccag tttggcgttg
    14581 ggcaggacga gaatgcctgt gttggtttct ttcacggctt tttgggcggc acttgcgtct
    14641 ttttcttcga caatggcggt tacgctgccg ctgccgtcgc ggatgatgcg ccccaaacct
    14701 gacgggtcgg cgggcacgtc ggtcaacagc ccgacttcgt tgcctgcggc ttcgagcagg
    14761 gtttcgaggg tttcaacgtc aattaaagga acgtcgccgt acaacaccag cgtgcggcct
    14821 tcggcggcaa ggtggggcag ggcggttttg acggcgtggc cggtgccgag ctgttcggtt
    14881 tgttcaaccc agacgacatc gcgtttgacg gtgtccaaaa cttgctcttt gccgtggccg
    14941 acgacgacgc agatgttttg cggattcagc gcggcggcgg tgtcgataac gcgcccgagc
    15001 attgtttcgc cgccgatttc gtgcaaaacc ttgggcattt tggaatacat gcgcgtgcct
    15061 ttgccggcgg cgaggatgac gatgtttaaa gtgttttgcg gcatagcgtt ttcctgtgca
    15121 atgccgtctg aaagggcttc agacggcata gggtgggttt atcggttttg aaatttgggc
    15181 ttttgccagt gttggcgatg ctcttcgtcg gcgttgttgc cggtttggtt gggtaacacg
    15241 gcatggcgtt cgggacggta ttggttgtag ttcatatttt tcgagtagct gccgtcttgg
    15301 taataaacgg gcgtgccggc gggatatttt tgacggacgg cggtcttgcc gttgccgtct
    15361 tgataagttt cccacgcgca gcccgacaaa agggcggcgg cagtcatcag gataaggaag
    15421 gttttacgca taacattctt tcgtattttc gggggggtgt gcgggtattg taatgatttt
    15481 ggcggtgttc tgacaaagtt tctgcatacc gagccagttg cgccatatcg cttacggaag
    15541 catcgataaa gggcaggtcg tgcacttttg caccgaaccg gacggttttc atgcccaatg
    15601 cttttgcctg atgcaggttg tccgcgctgt cgtccaccat aatgcagcat tcgggcggta
    15661 cgtccaaaag gcggcagaca ttgagatacg cttgcggatt gggtttgtac agcagcccga
    15721 aatcatccgt gccgaacagt gcgtcgaaac ggttttccaa accgagtgcg ccgacaacgg
    15781 cacggacgta aaacgacggg ccgttggaaa aaaccgcctt gcgcccgttc agacggcata
    15841 gggtgttttc cgtatccgcc atgccgtgca gcctggtcag gattgcatcg atcggatggc
    15901 tttcgcgcaa aaattcggcg atgtcgattt cgggatggtg gatttgcagt ccggcgagcg
    15961 ttgcgccgta gcggtgccaa tagtcttgac gcaggtcgga cgcggcagat tcggagagtt
    16021 tgaggcggcg tgccatatag cgtgtcatag cgcggttgat gagtgtgaag aggccggcat
    16081 cggcattgtg cagcgtgttg tcgaggtcga acagccacac ggtcgggttt tcttgcattt
    16141 tgaaccgtga aaatttgtta gaatgttatt ttacagcgaa tagaggagga ctcggaatga
    16201 aacggaaaat ttggctgctg ccgctgctgg cggtttcggc atacctgcag gcgcagacgg
    16261 aagtcaggct ggcggtgcat aagtcgttca gcctgcccaa agggctgatt gcgcgtttcg
    16321 agcgggcaaa cgatgcgaaa gtgtcgatta ttcaggcggg cggcgcgaac gaaatgctca
    16381 acaaactgat tttgagccgc gccaacccga ttgccgacgc ggtgtatggt ttggacaacg
    16441 ccaatatcgg caaggcgcgg gaaatgggca ttttggcggc ggcgcaaccc gaatccgccc
    16501 ccgtcgcggt cgggctgcct tcggctttgg cggtcgatta cggctatgtg gctatcaatt
    16561 acgacaaaaa atggtttgaa ggcaaaaagc tgcccctgcc gcaaaccctg caggatttga
    16621 cccgccccga atataaaaac ctattggtcg taccgtctcc cgccacgtcg tccccggggc
    16681 tgggcttcct gatggcgaac atcagcggtc tgggcgaaga aggcgcgttc aaatggtggg
    16741 cacagatgcg gcagaacggc gtgaaggtcg ccaaaggctg gagcgaggcg tattacaccg
    16801 acttttcgca caacggcggc gcatatccgc tggtggtcgg ttatgccgcc agtccggcgg
    16861 cggaagtgta tttttccaaa ggcaaataca gcgagccgcc gacgggcaac ctgtttttaa
    16921 aaggcggcgt attccgccag gtcgaaggcg cggcagtctt gaagggcgcg aaacagccga
    16981 agctggcggc aaaactggtg caatggctgc aaagtcggga agtgcagcag gcggttccgt
    17041 ccgaaatgtg ggtttacccc gccgtcaaaa acacgcgcct gcccgacgtg ttccgcttcg
    17101 cccaagcccc gacgcacacc accgcccccg cgcagcgcga tattgatgcg aaccagcgcg
    17161 gatgggtttc ccgttggatt agaacggttt tgaaataaaa caaacatacc gccccgcagg
    17221 gcttcatacg gcatttttac acctgtgccg attacgccgc atggggcgga tgttcgatca
    17281 agaggaaaac aatggacttc aaacaatttg attttttaca cctgatcagt gtttccggtt
    17341 gggagcatct ggctgaaaag gcgtgggcgt tcgggctgaa ccttgccgcc gcgctgctta
    17401 tttttttggt cggaaaatgg gcggcgaaac gcattgtcgc cgtgatgagg gcggcgatga
    17461 cgcgcgcgca ggtcgatgcc acgctgatta gttttttgtg taatgttgcc aatatcggct
    17521 tattgatttt ggtgattatt gccgcattgg gcagattggg cgtttccaca acatccgtaa
    17581 ccgccttaat cggcggcgcg ggtttggcgg tggcgttgtc cttgaaagac cagctgtcca
    17641 attttgccgc cggcgcgctg attatcctgt tccgcccgtt caaagtcggc gattttatcc
    17701 gcgtcggcgg ttttgaagga tatgtccgag agattaaaat ggtgcagact tctttgcgga
    17761 cgaccgacaa cgaagaagtc gtgctgccca acagcgtggt gatgggcaac agcatcgtca
    17821 accgttccac cctgccgctg tgccgcgccg aagtgatagt cggcgtcgat tacgactgcg
    17881 atttgaaagt ggcgaaagag gcggtgttga aagccgccgt cgaacacccc ttgagcgttc
    17941 aaaacgaaga gcggcaggcc gccgcctaca tcaccgcctt gggcgacaat gccatcgaaa
    18001 tcacattatg ggcttgggca aacgaagcag accgctggac gctgcaatgc gacttgaacg
    18061 aacaagtggt cgaaaacctc cgcaaagtca atatcaacat cccgttcccg caacgcgaca
    18121 tacacatcat caattcttaa acgccgtctg aaaggggagt gggaaatgga cgcgctgcac
    18181 accatcgccc gaaacctgac gaaaaaacgt caaaccgtaa gctgtgccga atcctgcacg
    18241 ggtgggctgc ttgccgccgt attcacaagc gttgcaggca gttcgcaatg gttcgatcaa
    18301 agttttgtaa catacagcaa caaagccaaa gaagaccgct tgggcgtgtt gcccgaaacc
    18361 ctgctcgaac acggcgcggt cagccgccaa accgtctatg agatggcgcg cggcgcgaaa
    18421 gccgtggcgc aggcggatta cgccgtcggt atttccggca tcgccggtcc gggcggcggc
    18481 agcgaaagca aacccgtcgg cacggtttgg ttcgggtttg cctttccggg cggaagttgc
    18541 gaagcaatgc gccgttttga cggcaaccgc gaatccgtcc gcgcgcaggc ggtcgccttc
    18601 gcgttggaac ggttggcggg gctgattgaa aacggcggcg atgccgtcta aacaaaatct
    18661 ccgtctggac aaaatccccg ccggataaaa aataccgtct gaaacgttgc aggtttcaga
    18721 cggtattttg tcgggttagg cggcggtgcg gcttatttca ctttactctt caacgcgccg
    18781 tagcctgccg cgtccatttg ttccagcggg atgaatttca agctcgcgcc gttgatgcag
    18841 tagcgcagtc cgcctttgtc gcgcgggccg tcggggaaga cgtgccccaa gtgcgaatcg
    18901 gcggcgtggc tgcgcacttc gatgcggcgc atattgaagc tgaaatcatc gtgttcggta
    18961 acggattttg catcaatcgg gcgcgtgaag ctcggccagc cgcagccgga atcatatttg
    19021 tcggcggagc tgaacaaagg ttcgccgctg acaacgtcca cataaatgcc gggtttgaac
    19081 aaatggtcgt attcgtggct gaaggcatat tcggtcgcgc tgttttgggt aacttggtat
    19141 tgctcttcgg tcagggtgcg tttgagttcg gcgtcactcg gttttttata cgttgccgcg
    19201 tcgaagcctt tgccttgcgg tgcggtcttg gttttgcccg gcagcggttc gtcggctttg
    19261 cggatatcga tgtggcagta gccgttgggg tttttaatca agtagtcctg atggtattcc
    19321 tcggcatcgt agaagttttt cagcggctcg ttttcaacaa cgaggggcag ttggtatttt
    19381 tgctgctcgc gtttgagggc ggcggcgatg acggcttttt cggcggggtc ggtgtagtac
    19441 acgccgctgc ggtattgcgt accggtgtcg ttgccctgtt tgttgaggct ggtcggatca
    19501 acgacgcgga agaaatattg caggatgtcg tctaggctga gtttgtcggc atcgtaggtc
    19561 actttgacgg tttcggcgtg gcccgtatgg cggtaggaca cgtcttcata gctcggattt
    19621 ttcgtgttgc cgttggcgta gccggatacc gcgtcaacca cgccgtcgat gcgttggaaa
    19681 taggcttcca agccccagaa gcagccgccg gcgaggtaaa tggtgcgcgt gttcatgatt
    19741 tttgaatcct ttttctgagt gtcgggtttg tagaacgaat gtttcaagct gcccaaatcg
    19801 gcattcgggt cgcggattaa cgccaacgcc tgcgcttcgt tgatgctgcc tttgacgatg
    19861 cgctgcacgt cgccgtcttt accgattaac gcccacgagg ggtaaacgct gatattcagg
    19921 ctttgggcga tcgtgccgcc gttgtcggtt acgacgggca gcttgggata attcaaaccg
    19981 gcataccatt tttggaagtc gccgtctttt ttctcgtgca aaaagcccgg agacgcgacg
    20041 gtaatcaggt tggcggagct gaattttgca tcttgcgccc atttttcggt ctgtcccaat
    20101 tcggacagac acaaaggaca ccagctcgcc caaaatttaa tcagcgtcgg tttgtctttt
    20161 ttcaagtaaa cactggcggg gcggttgtct gcggttttca aagtggataa agtgtgcggc
    20221 acggtcgcgg ctccggcatc gacgattttg ggcgaacaag cacccagcgc aagcaggcag
    20281 ccgaacttgg cgcaaaggga aaagaaagta cggtgtttca ttttgagttt cctgtgtgga
    20341 cggtttgcat gattagacgt ttgagatgcc gaaaccttac agctcggatt ttcagacaac
    20401 cttgccgcgt aaaatacgct acaatacgcc ctgtttcaag tttctaaaat taaaaggaaa
    20461 attcaatgtt cagcttcttc cgtcgcaaga aaaaacagga aacgccggct ctcgaggagg
    20521 ctcaaattca ggaaaccgca gcaaaagcag agtccgaaac cgatcaagta attgaaaata
    20581 ttaaagaaga tgctgaatct ttagcagaaa gcgtcaaagg gcaggtcgaa tctgccgttg
    20641 aaaccgtcag cggtgcggtt gaacaggtaa aggaaaccgt tgccgagatg ctgtctgaag
    20701 cagaggaagc ggcggaaaaa gcagcggaac aagtcgaagc ggcaaaagaa gccattgccg
    20761 aaaccgtcgg cgaggctgtc gggcaagttc aagaagccgt tgcgacaact gaagaacaca
    20821 agctcggttg ggcggcgcgt ttgaaacaag gtttgaccaa atcgcgcgac aaaatggcga
    20881 aatcgctggc gggcgtgttc ggcggcggac aaatcgacga agatttgtac gaagagctgg
    20941 aaaccgtgct gattaccagc gacatgggca tggaagccac cgaatacctg atgaaagacg
    21001 tgcgcgaccg cgtcagcctc aaagggctga aagacggcaa cgaattgcgc ggcgcgttga
    21061 aagaagcctt gtacgacctg attaagcctc tggagaaacc tttggttttg cccgaaacca
    21121 aagagccgtt tgtcatcatg cttgccggca tcaacggcgc gggcaaaact acgtctatcg
    21181 gtaaactcgc caaatatttc caagcgcagg gcaaatctgt gctgcttgcc gccggcgaca
    21241 ccttccgcgc cgccgcccgt gagcagcttc aagcttgggg cgaacgcaac aacgtaaccg
    21301 tgatttcgca aaccacgggc gattccgccg ccgtgtgctt cgatgccgtc caagccgcca
    21361 aagcgcgcgg catcgacatt gtgctggccg acaccgccgg ccgcctgccc acgcagcttc
    21421 atttgatgga agaaatcaaa aaagtgaaac gcgtgctgca aaaagccatg cccgacgcgc
    21481 cgcacgaaat catcgtcgtg cttgatgcca acatcgggca aaacgccgtc aaccaagtca
    21541 aagcctttga cgacgcatta gggctgaccg gtttaatcgt gaccaaactc gacggcacgg
    21601 caaaaggcgg catcctcgcc gcgcttgcct ccgaccgccc cgtccccgtc cgctatatcg
    21661 gcgtgggcga aggcatagac gatttgcgcc cgtttgacgc gcgcgcgttt gtggacgcac
    21721 tgctggattg agccgaaatg ccgtccgaaa acagcagacc gatgccgtca ttcccgcgca
    21781 ggcgggaatc cagaccttgg gataacggca atattcaaag tttataaaag acccgtcatt
    21841 cccgcgcagg cgggaatcta gaccttggga taacagcaat attcaaaggt tataaaagac
    21901 ctgtcattcc cgcgcaggcg ggaatctaga accatcggca cggaaactta tcgggtaaac
    21961 ggtttcttga gattccgcgt cctagattcc cactttcgtg ggaatgacgg gattttaggt
    22021 ttctgatttt g
//
LOCUS       DAWKQB010000016        21975 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.016, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000016 DAWKQB010000000
VERSION     DAWKQB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21975
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.016"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1188)
                     /locus_tag="WJL14_000476"
     CDS             complement(<1..1188)
                     /locus_tag="WJL14_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ7655597.1"
                     /translation="MSTFPNHTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHN
                     GKEALTAIDTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALESAGFVHYETSAFAKPAMQCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQNRPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPTAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQ"
     gene            complement(1221..3746)
                     /gene="ligA"
                     /locus_tag="WJL14_000477"
     CDS             complement(1221..3746)
                     /gene="ligA"
                     /locus_tag="WJL14_000477"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ7655598.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNVKTVANIPLRLHGENTPELIEVRGEVLMLKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLTFYEQMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVLHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPVKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSILSEAELLTLLG"
     gene            complement(3894..5180)
                     /locus_tag="WJL14_000478"
     CDS             complement(3894..5180)
                     /locus_tag="WJL14_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ7655599.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKSQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVPEPQTGHSAPKPADAPAKPVPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAHSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            complement(5374..5946)
                     /gene="ampD"
                     /locus_tag="WJL14_000479"
     CDS             complement(5374..5946)
                     /gene="ampD"
                     /locus_tag="WJL14_000479"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ7655600.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPNGHPFFSLIHTLRVSSHFLIKRDGKTVQFVSCGDMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLETLLEALCRRYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFPVDRNAV"
     gene            6030..7025
                     /gene="mltG"
                     /locus_tag="WJL14_000480"
     CDS             6030..7025
                     /gene="mltG"
                     /locus_tag="WJL14_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ7655601.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            7085..7705
                     /locus_tag="WJL14_000481"
     CDS             7085..7705
                     /locus_tag="WJL14_000481"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ7655602.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDRHFGC"
     gene            7927..9207
                     /locus_tag="WJL14_000482"
     CDS             7927..9207
                     /locus_tag="WJL14_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ7655603.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEIL
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            9443..9714
                     /locus_tag="WJL14_000483"
                     /pseudo
     CDS             9443..9714
                     /locus_tag="WJL14_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9754..10788
                     /locus_tag="WJL14_000484"
     CDS             9754..10788
                     /locus_tag="WJL14_000484"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ7655604.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGNLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            10994..11575
                     /locus_tag="WJL14_000485"
     CDS             10994..11575
                     /locus_tag="WJL14_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ7655605.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            11644..11826
                     /locus_tag="WJL14_000486"
     CDS             11644..11826
                     /locus_tag="WJL14_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ7655606.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            11823..12584
                     /gene="kdsB"
                     /locus_tag="WJL14_000487"
     CDS             11823..12584
                     /gene="kdsB"
                     /locus_tag="WJL14_000487"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ7655607.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGIEAVMTSNRHESGTTRLAEAAAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            12607..12924
                     /locus_tag="WJL14_000488"
                     /pseudo
     CDS             12607..12924
                     /locus_tag="WJL14_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            13029..13226
                     /locus_tag="WJL14_000489"
     CDS             13029..13226
                     /locus_tag="WJL14_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655608.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            13383..13475
                     /locus_tag="WJL14_000490"
     tRNA            13383..13475
                     /locus_tag="WJL14_000490"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13417..13419,aa:Ser,seq:gct)
     gene            13900..14685
                     /locus_tag="WJL14_000491"
     CDS             13900..14685
                     /locus_tag="WJL14_000491"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ7655609.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVAEAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            14722..15594
                     /locus_tag="WJL14_000492"
     CDS             14722..15594
                     /locus_tag="WJL14_000492"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ7655610.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(15654..16265)
                     /locus_tag="WJL14_000493"
     CDS             complement(15654..16265)
                     /locus_tag="WJL14_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ7655611.1"
                     /translation="MRRTALLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNDKLSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHDK"
     gene            16374..16598
                     /locus_tag="WJL14_000494"
     CDS             16374..16598
                     /locus_tag="WJL14_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ7655612.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            16599..17204
                     /locus_tag="WJL14_000495"
     CDS             16599..17204
                     /locus_tag="WJL14_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655613.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            17264..18298
                     /gene="pyrC"
                     /locus_tag="WJL14_000496"
     CDS             17264..18298
                     /gene="pyrC"
                     /locus_tag="WJL14_000496"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ7655614.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDQATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFVGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(18664..19089)
                     /gene="nusB"
                     /locus_tag="WJL14_000497"
     CDS             complement(18664..19089)
                     /gene="nusB"
                     /locus_tag="WJL14_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ7655615.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(19167..19643)
                     /locus_tag="WJL14_000498"
     CDS             complement(19167..19643)
                     /locus_tag="WJL14_000498"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ7655616.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(19690..20013)
                     /locus_tag="WJL14_000499"
     CDS             complement(19690..20013)
                     /locus_tag="WJL14_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ7655617.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            20180..20899
                     /gene="rnc"
                     /locus_tag="WJL14_000500"
     CDS             20180..20899
                     /gene="rnc"
                     /locus_tag="WJL14_000500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ7655618.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFAERVRRVDFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            20946..21869
                     /gene="era"
                     /locus_tag="WJL14_000501"
     CDS             20946..21869
                     /gene="era"
                     /locus_tag="WJL14_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ7655619.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         4848 a         5384 c         6522 g         5221 t
ORIGIN      
        1 ctgcagcaaa tcgtttaaaa acaagcgtcc tttttcggtc gggcggaata cggcggggtc
       61 ggtttccagc aggccttttt gccttgccgt ttcgatttgc gccatgattt tggcactcgg
      121 tacgcccgtg cgctcctgca acatcgcggt gggtacgccg tcggtcaggc gcagggcgtt
      181 catcatgaat tcgaacggca aatcttcggc ggcgacggtt ttgcgttcga cggcttcgct
      241 cggtcggttt tgcattaagg cgaggtagtc gttggggtgg cggcggcgga cggtgcgctc
      301 gatgcggtcg ggataggaaa ttttgccgtg cgcgcccgcg cctatgccta aataatcgcc
      361 gaactgccag tagttcaaat tgtggcggca ctgcatggct ggtttcgcaa aagccgatgt
      421 ttcgtagtgg acaaaacccg cgctttccag cgcgccgtgt accgcgtctt cgatgtcgag
      481 ggcggcttcg tcttgcggca aacctttcgg cggcgtatga ccgaacggcg tgttcggttc
      541 catcgtcaga tgatacgcgc tgatgtgggt cgcacccgta gcgatggcgg tttgtacgtc
      601 gtccaatgcc gtctgaacgg tttggttcgg cagggcatac atcaagtcga tattgacttt
      661 atcaaataat ttcaaggcgg tatcgatagc ggttaaggct tccttaccgt tgtggacgcg
      721 ccccagcctt gagagcatat cgtcgttgaa actctgtacg ccaatagaaa gccgcgtgat
      781 gcctgcgtct ttaaatcctt gaaacttctc gatttcaaac gtccccggat tggcttccaa
      841 cgtaatttcc gcttccggct gcaagcgcaa cagcgaacgc acgccgctca acaaacggtc
      901 aatcgattcc gcctgaaaca ggctgggcgt accgccgccg aaaaagatcg tttccaccgg
      961 tctgccccaa atattgggca attcaagctg caagtcggtc agcagcgcgt cgatataggc
     1021 ggcttcgggc aatccgtttt tcaggctgtg ggaattgaag tcgcaatacg ggcatttttt
     1081 gatgcaccac gggatgtgga tgtaaagcga caggggcggc agggcggtga gtcgggtgcg
     1141 gtttggaaag gaaatggtgt gcatggtgtg gttcggaaaa gtggacaatg ccgtctgaag
     1201 gcggttcaga cggcacaggt tcagccgagc agggtaagca gttcggcttc gctgaggatg
     1261 gaaacaccca aggcattggc tttttccagc ttgctgcccg cggcttctcc agcgacgacg
     1321 taatcggttt ttttggaaac gctgccggaa actttgccgc ctgcggcttc gattagggat
     1381 tgggcttggt cgcgtttgag ggtgggcagt gtgccagtta acacgaaggt tttgcccgct
     1441 acggctttat tgatgccgtc tgaaccttgc gccgcctcgt cttcagacgg catttgcgcg
     1501 aagaaggttt tcaggttttc gagtagggcg gcgttttgtg gttcgctgcg ccatgcttgc
     1561 cagtcggcgg ggagggcttt gtcgttttgt agaccttcta tgtttttacc agcgagttcc
     1621 cataaggctt gggctttgtt ttcgctaatt ttgaagtcgg gcaggcgggc gatccagcgt
     1681 tgcggttcgg cgtggcgggc gggcgggatg gtaacggctt gggtttgcgg ggcaacgcct
     1741 gcggcgagca gttcgtctat catcgcctgc tgttcggctt gggcgaagaa gtgggcaatg
     1801 gaacgcgcca ctaccgtacc gatgtcgggc aggcaggcga ggacgggttc gggtgcgcgg
     1861 cggacgcgtt ccaatgtgcc gaatgcctgt gccagcgttt tggcggtgcg ttcgccgacg
     1921 tggcggatgc cgagcgcgaa caggaagcgc gcgagttcgg gcgttttgcc ggattctatg
     1981 cctgcgagga tgttttccgc ccacttgacc ggttgttttt tatgtttgcc cgacgcgccg
     2041 accgaactgc cttcagacgg catttgatcc gattcggcaa cggttttgtc cgccgtttcc
     2101 ttcatttttt gcagggtcgg aatatcgagg cggtagaggt cggcgaagtg gaggacgagg
     2161 tcttgggcga ccagctgttc gatttgtttt tcacccaagc cgtcgatgtc catggctttg
     2221 cgcgaggcga agtggattaa gccttgcgcg cgttgtgcct gacaaagcat accgccgctg
     2281 catcgggcga cggcttcgcc ttcttcgcgt tcgatttcgc tgcggcagat ggggcagtgg
     2341 gtcggcaggc ggtagggctt gtggagcgga acggattggg tttgattggc ggacggtgtt
     2401 tcggcaaaca aatcgtcctg ccgatgcccg atgccgtctg aaacagcaac ggcggtttcc
     2461 cgcatcgggc ggcgttcaaa aatcacgcgc acaacttcgg gaatcacgtc tccggcacgg
     2521 cgtacgacga cggtatcgcc gacgcgaacg tctttgcgcg atacttcgtc ctgattgtgc
     2581 agggtggcgt tggtaacggt tacgccgccg acgaatacgg gttgcaggcg ggcgacgggg
     2641 gtaaccgcgc cggtgcgccc gatttgtacg tctatggctt cgacaatggt cagggcttct
     2701 tcggcaggga atttgtgggc aaccgcccag cgcggcgcgc gggagatgaa gccgagttcg
     2761 cgctgttgcg ccaagctgtt gactttgacc accatgccgt cgatttcgta gggcagttcg
     2821 gggcgttttt gctgcatttg ttcgtaaaac gtcaatactt cgtcgatatt tttgaaacag
     2881 ccgaaattgc cgttgggcag actgaagccg agtgcttgga aataggcgag ttcttggatg
     2941 tgttcttccg cgacgaaacc gccttgctga cgggcgacgg agtaggggaa aaagtgcaat
     3001 ttgcgttgcg cagtgatgcg cgaatcgagt tggcgtaggc tgccggcggc ggcgttgcgc
     3061 ggattggcaa agggtttttg cccgttttcg gcttgtcttt tattgagggc ggcaaaatcg
     3121 gctttgagca tcagcacttc gccgcgcact tcgatgagtt cgggcgtgtt ttcgccgtgc
     3181 agccgcaggg ggatattggc gacggttttg acgttttggg taacgtcttc gcccgttgtg
     3241 ccgtcgccgc gcgttgccgc ctgtaccaat acgccgtcgc ggtagagcag gctgatggcg
     3301 aggccgtcga atttgggttc gataacgtat tcgggtttgc cgccgtccaa accgtcgcgc
     3361 acgcgttggt cgaaagcgta catttctgca tggtcgaaca cgccgttttc atcttgcggg
     3421 gaaaaggcgt tggtcagcga cagcatcggc acttcgtggc gtacttcggc aaatcccgcc
     3481 agaggctcgc cgccgacgcg ctgggtcggg ctgtcgggca gtttgagctc gggatggttt
     3541 cgttccaacg cttcgagctc gcggaataat ttgtcgtatt cggcatcggg tacgctgggc
     3601 gcgtcgaggg tgtagtattc gtaggcatag cggttgagca ggtcggtgag gtggcggatg
     3661 tgttgtgcgg caaattgttt tatatcacta tcagacggtt taagaagatt ggtaaagtta
     3721 gtgttatgtt ttgagtttgg attcatgaga gaaggttttc agacgacctt tgtctgatac
     3781 gggatgaaac gggcaaaggt cgtctgaaaa atgataggtt gaaaacagct gaattttacc
     3841 cgaaaaaaag cggatatgcc gtaacgacat atccgctttg attgcattcg attttaggag
     3901 aacaggcgca atgcggtttt gccgcccggt tcgataccga ctttgagcat ctcggactga
     3961 cgagccaata cataagtgcg cacgtctttg agccattggg tcgaaacttc ttccattttg
     4021 tcgttgacca gattcaggtt caactggccg gacaggcgta ccgccaaatc cataaacaaa
     4081 tcgtcgaagg ttttttcgcc tgccggagag tgcgggatgt cgagcagcat actgaagcct
     4141 ttataggact ggttgtccaa aagggcattg gtaaacggct cgttgttgag cgagcagatg
     4201 gagaacatgg tcgagcccga cgtgtcggta tagtggaacg cgccgtcgtc ttccaaaacg
     4261 aaacccacgc ccgttacggc ggaacgcagt tctacgccgc tgatgctggt cggggaaacc
     4321 aaatggatgg cgatggtctg gtcgacgcgc gcgcagaatg cgtccagtgc ggaagccact
     4381 tcgataaagg cggcaaggtc ggtgtgcagc gtctgaccgc ccatgctgtg tgcgaatgca
     4441 tccacctggc ggttgaatgc ggagagttct tcctgcgagg caagtccgtt gcggctgact
     4501 gcctgaatac ccacgataaa tgcctgatag cggatgcccg ggatgggttc ggcaatctgg
     4561 aaatggtcgt ccatggtgca gccgacaatc tggtagcggc agcggttgga aaggcgcggc
     4621 agtgcgtgca gttctttggc ttcggtcagc gcgatataag agatgaagtc gaagcgcacg
     4681 tcaaaccagg gcagctcgac cttcgacagc tctttgagcg taatcagcgg ttttgccggc
     4741 gtttgcggaa cgggaacagg ttttgccggc gcgtcggcag gttttggtgc ggaatgtccg
     4801 gtttggggtt cgggaacggt gtgggcggag ttgccgataa tgccgctttc ttccaaggcg
     4861 gtttcgattt cggttttgaa cggggaggct tttgcctgtt tctgcttggc gatgtagacg
     4921 gcatcctgct cttgcaggtt gcgcatggcg gggtcttggg attttgccgt ttttttgacc
     4981 gccggttggg gtttcggcat catgactggc ccgccggacg gtttgccgtc gcggacatgg
     5041 ctggttttgc tgttgagcag ggcatctttg tcggagtgcc cgaactggtc gcgcactttt
     5101 ttgcggtatt ggttttcctg atacatattg taggcgacaa cggcgaggac ggcggcgagg
     5161 aacagtacga tgtaaatcat ggcaatcact ttaaatttcg ggatgcagga tgcgcaaagt
     5221 gcgggtactg cggttaaatc gggcttgcgt ttccggcagt ctgatggaac ggccggttat
     5281 aacgtttgaa ttataacgaa aattgcaggg tctgacagca gtgtgtcgaa ataagcggaa
     5341 attttccgaa atgccgtctg aaatctgtgg ttttcagacg gcatttctgt ctacgggaaa
     5401 ccctttctcc cgtatccgcc gccagtcgaa aaagtggccg gggtcggttt tgcggccggg
     5461 cgcgatgtcc tgatgtccgg ttactgcggt aacggggtag cggcggcaga gtgcttccaa
     5521 caatgtttcg agcgaacggt attgcgcttc ggtaaagggt tcgaaatcgc agccttccaa
     5581 ttcgatgccg atggaaaatg cgttgcattt ttcccgtccg cgaaacgagg atacgcccgc
     5641 gtggtacgcc atatcgccgc atgatacgaa ctgcaccgtt ttgccgtcgc gtttgattaa
     5701 gaaatggctg gatacgcgca aagtgtgtat caggctgaag aacggatgtc cgttggggtc
     5761 gagccggttg gcaaacagct tttccacagc atccgtgccg tattcgaacg gcggcagtga
     5821 aatgttgtgc aacacgatca gggaaaccgt ttcccccgtt tccctcgggc tgaaattggg
     5881 cgacggggta tggcgtatgc tttgaagcca gccgttttgc cagtgtgctt cggcgtgatt
     5941 gtccatgatg ttcttcctgt ccggcgggca atttgggtta tactgtcgcc cgaattttaa
     6001 gacgtattcc gaatgctggg aatcctacca tgttgagaaa attgttgaaa tggtctgccg
     6061 tttttttgac cgtatcggca gccgttttcg ccgcgctgct tttcgtccct aaagacaacg
     6121 gcagggcata caggattaaa attgccaaaa accagggtat ttcgtcggtc ggcaggaaac
     6181 ttgccgaaga ccgcatcgtg ttcagcaggc atgttttgac ggcggcggcc tacgttttgg
     6241 gtgtgcacaa caggctgcat acggggacgt acagactgcc ttcggaagtg tctgcttggg
     6301 atatcttgca gaaaatgcgc ggcggcaggc cggattccgt taccgtgcag attatcgaag
     6361 gttcgcgttt ttcgcatatg aggaaagtca tcgacgcaac gcccgacatc gaacacgaca
     6421 ccaaaggctg gagcaatgaa aaactgatgg cggaagttgc ccctgatgcc ttcagcggca
     6481 atcctgaagg gcagtttttc cccgacagct acgaaatcga tgcgggcggc agcgatttac
     6541 ggatttacca aatcgcctac aaggcgatgc aacgccgact gaatgaggca tgggaaagca
     6601 ggcaggacgg gctgccttat aaaaaccctt atgaaatgct gattatggcg agcctgatcg
     6661 aaaaggaaac agggcatgaa gccgaccgcg accatgtcgc ttccgtcttc gtcaaccgcc
     6721 tgaaaatcgg tatgcgcctg caaaccgacc cgtccgtgat ttacggcatg ggtgcggcat
     6781 acaagggcaa aatccgtaaa gccgacctgc gccgcgacac gccgtacaac acctacacgc
     6841 gcggcggtct gccgccaacc ccgatcgcgc tgcccggcaa ggcggcactc gatgccgccg
     6901 cccatccgtc cggtgaaaaa tacctgtatt tcgtgtccaa aatggacggt acgggcttga
     6961 gccagttcag ccatgatttg accgaacaca acgccgccgt tcgcaaatat attttgaaaa
     7021 aataaaccat gccgtctgaa aagtttgtgt tttcggacgg cataccctta ccggaactgc
     7081 aagcatgaaa ccgcaattca tcactttgga cggcatagac ggtgccggca aatccaccaa
     7141 ccttgccgtc atcaaggcat ggtttgaacg gagggggctg cccgtgctgt tcacgcgcga
     7201 accgggcgga acgccggtcg gtgaggcctt gcgcgaaatc ctgctcaacc ctgaaaccaa
     7261 agccggtttg cgtgcggaaa cactgatgat gttcgccgcc agaatgcagc atattgaaga
     7321 cgtgatattg cctgcacttt cagacggcat ccatgtcgtg tccgaccgtt ttaccgatgc
     7381 gaccttcgcc tatcagggcg gcgggcgggg aatgccgtct gaagacattg aaattttgga
     7441 acattgggtg cagggcggtt tgcgccccga tttgaccctg ctgttggatg tgccgctgga
     7501 agtgtcgatg gcgcgtatcg ggcagacgcg cgagaaagac cggttcgagc aggaacaggc
     7561 agatttcttc atgcgtgtgc gcagcgttta tctgaaccgt gccgccgcct gtccggaacg
     7621 ttacgccgtt atcgacagta acctcggctt ggatgaagtc agaaacagca tagaaaaagt
     7681 gttggacaga catttcggct gttgatgcgg caaatattga aacaagcgca tccgcccgcg
     7741 ccgaaaatca aacggcagtg ccgcaggtga aaatggcggt atgcgccaaa ctttcggcat
     7801 gatagaatta cgctcggtta caaggcagga tgcgtcggca atattaacga accgcccgta
     7861 acatgatgac ccgaaagcgt ttcggacagt ccgattcaaa tctttttctc gcaacaggat
     7921 tgacacatgg aaaactcatt gaaagaagcc gccctcaagt tccacgaatt acccgttccg
     7981 ggcaaaattt ccgttacccc gaccaaatct ctggcgaccg acaaagattt ggcgttggcg
     8041 tactctccgg gcgtagccgc tccttgtatg gaaatccatg ccgatccgca aaatgcctac
     8101 aaatacaccg ccaaaggcaa cttggtcgct gtcatttcca acggtacggc cgttttgggc
     8161 ttgggcgaca tcggcgcgct ggcgggcaaa cccgtgatgg aaggcaaagg cgtattgttc
     8221 aaaaaattcg ccggtgtgga cgtgttcgac atcgaaatcg atgaaaaaga cccgcaaaaa
     8281 ctcgtggaca tcatcgccgc tttagagccg accttcggcg gcatcaacct cgaagacatc
     8341 aaagcacccg agtgtttcta catcgaacgc gaattacgca aacgctgcaa aatccccgta
     8401 ttccacgacg accagcacgg cacggccatc attaccgccg ctgccgtatt gaacgccctg
     8461 cgttttaccg gccgtaaaat cgaagaagcg actttggtgt gttccggcgc aggtgcggcc
     8521 gcgattgcct gcttgaacca attgctggat ttgggcttga aacgcgaaaa cgtgaccgtt
     8581 tgcgactcca aaggcgtgat ttaccaaacc cgcgaagaca aagaccgtat ggacgagtcc
     8641 aaacagttct acgccattga agacaacggc cagcgcgtgc ttgccgatgc cgtcaaaggc
     8701 aaagacatct tcttgggcct ctccggcgcg aacctgctga cgcctgaaat actgaacacc
     8761 atgaacgaaa aacccatcgt gttcgctatg gccaacccga atccggaaat cctgccgccg
     8821 ctggcgaaag aaacccgtcc ggacgtggtt atcggtaccg gccgctccga cttcccgaac
     8881 caagtgaaca atgtattgtg cttcccgttc atcttccgcg gtgcgttgga tgtcggcgcg
     8941 acgaccatca acgaagaaat gaaacgcgcc tgcgtgtatg ctttggcgga tttggcgatg
     9001 gaagaggtaa ccgaagaagt ggttgccgct tacggtaaga aatttgaatt cggcgcggaa
     9061 tacctgattc cgactccgtt cgattcccgc ctgctgccgc gcgttgctac ggctgccgcc
     9121 aaagcagcga tggaaagcgg cgtggcaacc cgtccgattg cagatttgga agcttacgct
     9181 gccaagctga gcgaatggaa gctgtaagcc gtttgcggtt taaaatgccg tctgaactgt
     9241 tttcaggcgg cattttgctg tcagattgat atagtggatt aacaaaaatc aggacaaagc
     9301 gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgtt tcagcacctt
     9361 agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac
     9421 tataaatgaa agatactgaa aaatgaaaga gatgaaacct gtccgttatc atattggcga
     9481 tatgcccgag acttcaaaac aaaccgcctc ccgtcatgac gacagggcag tgggtgttga
     9541 cgatgattgt tttcatgatt cctttggtca atttttgttt gggtgttcgg cagaggcaac
     9601 ccgaaccgcg ccaatttctg taaagcgcag ttgcttattt acctgattgg ttcgcttatc
     9661 ggtttggtct tcgcgttgtt tataggtggg tctgtatcag gtacgcatga ttaatgcccc
     9721 gggctgattt tgcttcgagg atttgtatcg aatatgccga attgtttcaa atttcatacc
     9781 gttatcgaac ggcattggca aaaaccttat ccggttttgt cttttctgct taagccgctc
     9841 tccgggctgt ttgccaaaat tgcggcaaaa cggcggacgg attttttatc gggaaaacgg
     9901 caaagcgaaa agctgcccgt gcctgtggtc gtggtcggca atattcacgc gggtgggacg
     9961 gggaaaacgc cgattgttgc cgcgctggtg tcgggtttgc aggaaaaggg cgtcaaggtc
    10021 ggcatcatca gccggggcta cgggcgcaag agcaaggcgg ttcatgtatt gaatgctgag
    10081 agccgagcgg aagatgcggg cgatgagcct ttgctgctgt tccgcaaaac cggtgcgccg
    10141 acggcggtgg gcagcagccg tgcagaggca ggcagggcgt tgctggcggc gcatcccgac
    10201 atcggactga ttgtggcgga cgacggtttg cagcattacg ccctgcggcg ggatgtggaa
    10261 atcgcggtgt ttccggcggc ggatacgggg cgcacggatt tggatttact gcccaacggc
    10321 aatttgcgcg aacctttgtt gcggctggat tcggtggatg cggtcgtcgt cagcggcggc
    10381 aaggcggatg cgctgtttag gccgtctgaa aatatgtttc acagccgtat cgaagcggga
    10441 cggatttacc gtttgaacaa tccgtccgaa atactggaca caggccgtct gaaaaatcaa
    10501 accgtcgtcg ccgtggcagg tattgccaag ccggcgcggt tttttgattc gttgcggaat
    10561 atgggcatta ccgtgaagcg aaccgtcgcg ctgcccgacc acgccgacat ttcggcggca
    10621 gatttgcccg atgcggacgc ggtcattatt acggagaaag atgcggtcaa attttcagac
    10681 ggcatttgca ccgataatgt ttgggtgttg cccgtttgtg cgataatcga acctgatttg
    10741 gcggcgtttg tgttggagcg gttggaagat gtaccgaagg ccgtctgaaa gcacggtttg
    10801 ggcggagtga ttacggattt gaataagaac gcctcgcgcc gtcattcccg cgcaggcggg
    10861 aatctaagtc tcgaattttc aggaatgcct aggaggctcc agaaatccca aatctccgga
    10921 tttccacttg gacaggaatg agaaaaccgg tcgtattttt tatctgcatt aatcattcat
    10981 taaaggattg aatattaaac tgaaaacctt gttattgccc ttcgccacgc tggcattgtg
    11041 caccaatgct tttgccgccc cgcccagcga cgcgtcgttg gcgcgttggc tggatacgca
    11101 gaattttgac cgggatatag aaaaaaatat gattgagggc tttaatgccg gatttaaacc
    11161 gtatgcggac aaagcccttg ccgaaatgcc ggaagcgaaa aaagatcagg cggcagaagc
    11221 ctttaaccgt tatcgtgaga atgttttgaa agatttgatt acgcccgaag tgaaacaggc
    11281 tgtccgcaat actttattga agaatgcccg tgagatatac acgcaagaag aaattgacgg
    11341 catgattgcc ttttacggtt cgcctgtcgg tcagtccgtc gttgccaaaa atccgcgctt
    11401 aatcaagaaa tcgatgagtg aaatagcggt atcttggact gcattgtcag ggaaaatcgc
    11461 gcaacatcat ctgcccgagt ttacggaaga gttgcggcgc atcatctgcg gcggtaaaaa
    11521 tcccgatgcg ggctgtaaac aagccggaca ggttgggaaa aggcatcaga aataaatgat
    11581 agccgtctga aatattgaag agggcatccg attgattgaa ccatcaaacc cgaaagcaac
    11641 cctatggaaa aaaaattctt agacatcctc gtctgccccg ttaccaaagg caggctggaa
    11701 tatcatcagg acaaacagga attgtggagc cgtcaggcga agcttgccta tccgattaaa
    11761 gacggcattc cctatatgct ggaaaacgaa gcgcgagcgt tgagcgaaga ggaactcaaa
    11821 gcatgaccga attcgtcgta ttgattccgg cgcggctgga ttcgtcgcgc ctgcctggaa
    11881 aagccttggc ggacatccac ggcaaaccga tggtcgtgcg cgttgccgaa caggcggcaa
    11941 aaagtaaagc cgcgcgcgtc gtcgtcgcca ccgaccatcc cgatattcag acggtctgtc
    12001 aggcgcacgg catcgaagcg gttatgactt caaaccggca cgaaagcggc acgacgcgcc
    12061 ttgctgaagc tgccgccgcg ctgaagctgc cgccgcatct ggtggttgtc aacgtacagg
    12121 gcgacgagcc gctgattgcc cccgaactca tcgaccgcac cgccgaagta ctcgtcgaaa
    12181 acaacgtcca aatggcgacc gccgcccacg aattgcacga tttcgacgaa ttgatgaatc
    12241 ccaatgccgt caaagtcgtc ctcgacaaaa accgcaacgc catctacttc agccgcgccc
    12301 cgattcccta tccgcgcgat gcaatgcgtg ccggaaaacg cgaaatgccg tctgaaaccg
    12361 ccgtcctgcg ccatatcggc atctacgctt accgcgccgg cttcctgcaa cgctatgccg
    12421 aaatgagcgt ttcgccgctg gaaaccatcg aatcgctgga acagctgcgc gtcctgtggc
    12481 acggttatcc cattgccgtc gaaaccgcca aagaagcccc cgccgccggt gtggatacgc
    12541 aggaagattt ggacagggtc cgcgccgtat ttcagaccgt ataaaacagg ttcaaaggga
    12601 aaagatatgc agcaacatat tgaaaagtgg cagcacttga gccgtgaaga acagaaaatc
    12661 cttgctgaag tatggggtct cgtgcaaaac gacgatcagg aggttcacta tgaaatgctc
    12721 aaattgaacg cacccgacga agccagtggc gaattttggt tcagaatggc agaaacactc
    12781 agcaccctgc cgcccaaccg ttccctcgac cttagaatga acggcggcag gctggcgacc
    12841 gccgtatcca tcctttccgt catgattgaa gacaatcccg acataccgca gctttgggcg
    12901 caaaaaatta ccgcgctcaa ttatagtgga ttaaatttaa accagtacgg cgttgcctcg
    12961 ccttgtcgta ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc
    13021 cactatattt ggcacacgga cacaaagccc gtgccgacgg tttggcacaa cagcccgaca
    13081 aagcggcaga agccaacgag gaggaatacc tgaccaaagc cctgtcgcaa aacctgctgt
    13141 caacattgga tgtcgcgctt gcacgttttc ctgaagacgc gtggtttcag gaaatcaaac
    13201 aggatgcaca aaagcatttt gcttgaggat gtggcagtca ggaatatttc cattcaggaa
    13261 gaaaagaagt gcctgattgg gtataatcag ggtaaatctt attttatttc aaaagattaa
    13321 tatttgcttt ctgtttttcc ttgacggtat cggaaaagtt gattatagtt acagcttcct
    13381 taggagtaat ggctgagagg ctgaaggcac ttccctgcta aggaagcatg tggggtcaac
    13441 ctgcatcgag ggttcgaatc cctcttactc cgccagataa aaaatagacg ctgtgtttta
    13501 cagcgtctat tttttatgca attttatagc gggttggtgc aaaaccagta tggtattgcc
    13561 gtgtcttgat tctgaatttt gttatagtgg attaacaaaa accagtacgg cgttgcctcg
    13621 ccttagctca aagagaacga ttctctaagg tgctggagca ccaagtgaat cggctccgta
    13681 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataatc
    13741 cgagatgctt gccgtttatt tccgcctcgt tcaaacggcg gctctgattt gcgcggtttc
    13801 tgtttgccgt attcgcctat ccgtaccgca aatgttatac tgggaaaaat ttactgattg
    13861 tgttttacgg catatttgcc gataggatgg aagagacaaa tgagcagaat ccggcaggct
    13921 tttgccgctt tggatggcgg aaaggcattg attccctata ttacggtggg cgaccccgat
    13981 attcggacaa ctttggcatt gatgcacggc atggttgcaa acggtgcgga tattttggag
    14041 ttgggtgtgc cgttttccga tccgatggcg gatgggccgg ttattcagcg tgcggcggag
    14101 cgggcgttgg caaacgggat ttcgctgcgc gatgtcttgg atgtcgtcag aaaattccgt
    14161 gaaaccgaca cgcaaacgcc ggttgttttg atgggatatt tgaaccctgt acataagatg
    14221 ggttatcggg agtttgctca ggaagccgca aaggcgggtg tggacggcgt gttgacggtg
    14281 gattcccctg tcgaaaccat cgatccgctc tatcgcgagc tgaaggataa cggggtcgac
    14341 tgtattttcc tgattgcgcc gacgacgacg gaagaccgta ttaaaaccat tgccgagctg
    14401 gcaggcggat ttgtctatta tgtttcgctc aagggcgtaa cgggcgcggc aagtttggat
    14461 acggatgagg tttcgcgtaa aatagagtat ttgcatcagt atatcgatat tcccatcggt
    14521 gtcggtttcg gcatcagcaa tgcggaaagc gcgcgcaaaa tcggccgggt tgccgaagcg
    14581 gtcattgtcg gcagccggat tgtgaaagag attgaaaaca atgcgggcaa cgaggctgcg
    14641 gtcgtcggtg ctttggtcaa agagttgaag gatgccgtgc gctgattctg tcacgcattc
    14701 taaatatttt aggagttgtc catgagctgg ttagataaaa tcctgccacc caaaatcaaa
    14761 aaccgcagca aagacggttc ttccaatgtt cccgagggtt tgtggcacaa atgtccgtct
    14821 tgttcggcaa ccgtttattc gaccgagttg cagcagaaca atcaggtttg tccgaaatgc
    14881 aaccaccaca atccgttgtc ggcacgacaa cgcctgaatc tgcttttgga tgaggatggc
    14941 agagaggagg ttgccggaaa tgtcaaaccg acagatcctt tgaagtttaa agacggcaaa
    15001 aaatatccgg atcgtttgag tgcggcacgc aagctgaccg gggaagatga tgctttggtg
    15061 gtaatgaaag gcaagatgaa cggtctgccc gttgtggttg ctgcgtttga gttccgcttt
    15121 atcggcggtt cgatgggttc ggttgtgggc gaacgattcg tacagggcgt ccgccgggcg
    15181 gttgccgaca attgtccgtt cgtctgtgtg gcggcttccg gcggtgcgcg tatgcaggag
    15241 ggtgtgaact cattgatgca gatgacgaaa accagtgccg cgctgcattt gctgacggaa
    15301 aaacgcctgc cgtttatatc ggtgttgacc gatccgacta tgggcggcgt atccgccagc
    15361 ttcgcatttt tgggtgatgt tgtgcttgcc gaaccgaacg cgctgatcgg ttttgccggt
    15421 ccgcgcgtga ttgagcagac ggtgcgcgaa acgctgccgg aaggcttcca acgcgccgag
    15481 ttcctgctgg aaaaaggcgc aatcgaccag attgtcgacc gccgcgatat gaagcggcgc
    15541 atcagtgatt tgattacgct gttgtgccgt caggacaaag tttctgccgc ctgatggctg
    15601 atgaatcgaa taccgtctga aaccgatgtt tcagacggta tttttgtgtc tggttatttg
    15661 tcgtgcggct ttatcgacgg ggcatagcgt ccggcacgtt ctttcaggcg ttgtaccaaa
    15721 cctttcgtgt cggcgggtac accgccctcg cagaatgcct gatacaggac ggtgcgcagt
    15781 gcgtcgttgc ggcttaatgt accgcctatc ggtttccatt cggcgtttcg gggctgtatc
    15841 cagcggcggt tgaccgtgtc gccgtagccg aacactttgt aggaggaaag tttgtcgttg
    15901 ccgaacctga tggagaagcg ggcgcagaat atgccttcgg cagtcaggtt gtcgtagcct
    15961 ttgtcggagc ggatattgag aatgtagcgg atgctgccgt cgggcgcggg cataatttgc
    16021 aggctgtcga gcaggatttt cggctgtttg ccgtaatttt catccacata aatgtcgaac
    16081 cagccgtccg agtgcgtatc gggcagcggc ggcagtttgg cggtatgttc tttaaattcg
    16141 cgggcggcgg cctcttcggg cgtttcgcgg tagcgggtgt tgatcggcgt gtctttttgg
    16201 ctgaagccgg cagcgaggga cgtgccggcg gcgagtgcga gcagcaggag ggcggtgcgg
    16261 cgcataagtt tctccaaatt gaaaacggcg ttattttatg ggttggcaaa gggggctgca
    16321 agcaactggg gtataatctc cccccgattc ccatttttta aacggtacaa acgatgaaca
    16381 gcgaaacttt agacgtaacc ggattgaaat gtcccctgcc gattttgcgg gcgaaaaagg
    16441 ctttggcgca aatgcagcag ggcgacgtgt tgaccgttct ggcaaccgac ggcggcgcac
    16501 cgggggattt tgaggctttt tgccgccaaa ccggtcatgt gctgttggat gcttccgaac
    16561 aggacggcgt gttcacgctg gtcgtccaac acaaataaat gccgtctgaa atgcggatgt
    16621 cccgattgat gttattgctg tcggcatttg tgcttgctgc gtgtaatgcc caaaaccggc
    16681 aatccggtgt acggcatcag gatttcgcgc gtgtagccgt cgatttgttt cggagtgcct
    16741 gcgtcctgac tcaaggcagt tttgaatcgg tttccaagtt tgccgctgtc ggacattttg
    16801 tccctgtcgg aaaggaagac ctgtcccatc tgccgcccac tgttgcagaa cccgatgtac
    16861 aggcgttgtg gacgcttgaa cgcgatggcg gcatttatta tttgagcctg acgcgcgaca
    16921 gttgcagtgt gaaagccgag cgggcagaca gtgccgcgct gttggaacat tttgacggac
    16981 ttgtccgaca gccgccgaaa aatgccaata tagaattgag ggcggaacag tcggtgcagg
    17041 catcatttga aatccgccaa gtcagctatg cctggcggga agcgggcagc tccgaagaaa
    17101 ccgtattgac cgcacaaacc gccgattccc cggatttacc cgtacaggca gttttgaacc
    17161 tgacgcaccg ttcccacaac gggaaacccc taattctccc ctaacttcag acggcataaa
    17221 gtcggcatgc cgtctgaaca ttcaaataaa acaaaggaaa accatgcaaa ccctgaccat
    17281 tatccgcccc gacgatatgc acctgcacct gcgcgacggc gacgcgctca aagccgttgc
    17341 tccttatacc gcccgccaaa tggggcgtgc cgtcattatg cccaacctca aaccgcctgt
    17401 cgtcagcgtt gccgacgcgc ttgcctacaa agcgcgcatc atggcggcat tgcccgaagg
    17461 cagcgcgttt gagccgttga tgacgcttta cctgaccgac caagccacgc ctgcacttgt
    17521 gcgcgaagcc aaagccgccg gcatcgtcgc cttcaaactc taccctgccg gcgcgaccac
    17581 caattccgat tccggcgtaa ccgacctgtt caagctcatc cccgtgttgg aagaaatggc
    17641 gaaacagggc attttgttcc tcgttcacgg cgaagtaacc gaccccgaaa tcgacatctt
    17701 cgaccgcgaa gccgcctttg tcgggcgcgt gatgaaaccc gttttggcgc aagtgccgaa
    17761 tcttaaagtc gtgttcgaac acatcaccac cgccgaagcc gcccgcctgg ttttggaagc
    17821 aggcgacaac gtagccgcca ccgttacccc gcaacacctc ctgctcaacc gcaacgacct
    17881 cttggtcggc ggcgtgcgcc cccatcattt ctgcctgccc gtactcaaac gcgaaaccca
    17941 ccgtcaggca ttggttgccg ccgttaccgg cgagaaggcg cataaattct tcctcggcac
    18001 cgactccgcg ccgcacgcca aatccgccaa agaaaacgcc tgcggttgcg ccggtatgtt
    18061 cagcgcgatg accgccatcg agctttatgc cgaagtgttt gaaaaagcag gcgcgttgga
    18121 caaactcgaa gccttcgcct cgaaaaacgg cgcaaggttt tacggcattc ctgaaaatac
    18181 cgacacgatc accctcgtca aacaaagcca aaccgtcccc gcaagtattc cctacggcga
    18241 cggcgaactt gtcccgatgc gcgcgggcgg cgaaatcggc tggacggtac agtattgatg
    18301 gggctggaaa caaaatgccg tctgaaaggt ttttcagacg gcatttgtgt tgttgactga
    18361 ttgatacgtc aacggcgttt gagtttgtta cgtttcggtt atttccgata aattcccaca
    18421 attttcaaat ttcgccattc ccacgaaggc aggaatccgg aaattcgatg cgaccagagt
    18481 ttatcaaaaa cggcagcaac tcaaaaaacc ggattcccgc cggcgcggga atgacgagat
    18541 tgaagtttca gaatttattt gaaataccca aaattcaaaa aaccaaattc ccacctgcgt
    18601 gggaatgacg aaacaaagaa agcagaaata aggacataga actttcttta aatttgtgat
    18661 gcatcaacgg cgtttgggct cgtcgggacg gatttgggcg gcgagtttgt cgaggatgcc
    18721 gttgacgaat ttgtgcccgt ccgtgccgcc gaaggttttg gttacttcga tggcttcgtt
    18781 gatgatgacg gggtagggcg tttcgggcat ggcggacagc tcgtggcagg cggtcagcag
    18841 gacggcgcgt tcgatggggt tgaggtcttt ttcgtcgcgg tcgagcaggg ggcggatttg
    18901 tcggatgtac tctgccgcat tggtttgcgt gccgaagaaa agtttgttga acaattcttc
    18961 gtctgccttg gcaaagtcgg gcatttcgcg gatgttttta gcaatctcgg gcgcggcggt
    19021 gcggttgata agggattggt aaacggcttg tacggcaagc tcgcgggaac ggcggcgggc
    19081 tgttttcatg atttttcctt gaaacggttg ggcggcacgg tatgccgtct gaaacggaaa
    19141 gggcgtatcg gtgtacgccc tgtttgttat tcttcgtctt caaactgttc ttcgagcagg
    19201 aggttgacga ggttggcgca ttctacggcg actttggcgg catccgaggc tttttcttca
    19261 atccgttcga ttgcctgtgc gtcgttttcc gtggtcagga cggcattggc aatcgggatg
    19321 ttgtagtcga gtgcgacgcg gctgacccct gctccggatt cgttggaaac cagctcgaaa
    19381 tggtaggttt cgccacggat aacgacgccg atggcaatca gtgcgtcaaa tttttcagaa
    19441 gaggcaaagt tcatcagcgc gatggggatt tcaagcgcgc cgggtacggt ggcgacggta
    19501 atgttttcgt ctgccacgcc caattcttgg agggtgcggc agcagacttt gagcatttcg
    19561 ctgccgattt cgttggtgaa gcgtgcctgt acgatgccga tgcggaggtg tttgccgtcg
    19621 aggttggggg cgatggtgtt cattgagtgt cctttggtat tcggaggttt cggaatgccg
    19681 tctgaagggt cagtccttag gttgccagtc ggcgacggtt tggaatgtgc cgtcttctgc
    19741 caattcccac gcgctgcctt caggttggga gagcagtgcg gcggtttcag ggttggtttt
    19801 ggtgatatcg gcgaggctga cgatgctgaa gttgtccggg tcgtcggtgt attcgtcggt
    19861 ctcgtcgccg ctgaagaaac gccagccgct gtcgttttca aaaacggggg cttcgcggta
    19921 gaggaagccg acgggctggt tttgtttggc gacggtgttg gtggcgatgc agcggtcgag
    19981 tgccgaggaa agtgcttgtg caaatgcgtt cattgcggga atacgttggg ggaaaacttg
    20041 cggattttac cacgattctt gcgttgtcgg cagacggcgg cggtttggtg gtacaatgtg
    20101 cgccgtttgc agccttaagg tgtttctgta tttttggagt atggaaacgc attcgggctg
    20161 ttttttgcgg aagacggtaa tgaaagacga tgttttgaaa cagcaggcac acgcggcgat
    20221 acagaagaaa ctgggctacg cgttccgcga tatttcgctt ttgcggcagg ctttgacgca
    20281 caggagccat catgcgaagc acaacgagcg gttcgagttt gtcggcgatt cgattttgaa
    20341 ttatacggtg gcgcggatgc tgtttgacgc gtttccgaag ttgaccgagg gcgagttgtc
    20401 gcggttgcgg gcaagtctgg tcaatgaggg cgtgctggcg gaaatggcgg cggaaatgaa
    20461 tgtcggcgac ggcctgtact tgggggcggg cgagttgaag agcggcggct tcagacggcc
    20521 ttcgatactg gcggacgcga tggaggcgat gtttgcggcg gtcagcttcg atgcggattt
    20581 caacacggcg gaaaaggtgg tgcgccattt gtttgcagaa cgcgtccggc gagtcgattt
    20641 ccaaaatcag gcaaaagacg gcaaaactgc tttgcaggag gcgttgcagg cgcgccgttt
    20701 cgccttgccg aaataccgta tcgaagagca aatcggccat gccaacgaca gtatgtttgt
    20761 catttcctgc gatttgggcg aactgggttt cgtgtgccgt gccaaaggga cgagccgcaa
    20821 ggcggcggag caggaagcgg cgaaagaggc tttgaaatgg ctggaagaga agctgccgct
    20881 gaagaagaaa aagaaatgag gcggcgcgtg aatatgccgt ctgaaatatg gatacgaaag
    20941 caaatatgga tattgaaacc ttccttgcag gggaacgcgc cgccgacgga taccgttgcg
    21001 gcttcgtagc gattgtcggc cgtccgaacg tgggcaaatc aacgctgatg aatcatctca
    21061 tcggtcagaa aatcagtatt accagcaaaa aggcgcagac gacgcgcaac cgcgtaacgg
    21121 ggatttatac cgacgatacc gcgcagtttg tgtttgtcga tacgcccggt tttcaaaccg
    21181 accaccgcaa cgcgctcaac gaccgtttga atcaaaacgt taccgaggca ctcggcggcg
    21241 tggatgtggt ggttttcgtc gtggaagcga tgcgttttac cgatgccgac cgcgtcgtgt
    21301 tgaaacaact gcccaagcac acgccggtca ttttagtggt caacaaaatc gataaggaca
    21361 aggcgaaaga ccgttacgcg ctggaggcgt ttgttgccca ggtgcgcgcc gaatttgaat
    21421 ttgcggcggc ggaggcggtc agcgcgaaac acggattgcg gattgccaac ctgttggagc
    21481 tgattaagcc gtatctgccc gaaagcgtgc cgatgtatcc cgaagatatg gttacggaca
    21541 aatcggcgcg ttttttagcg atggaaatcg tgcgtgaaaa attgttccgc tatttgggcg
    21601 aggaattgcc ttatgcgatg aacgtcgaag tggagcagtt tgaagaggaa gacggtttga
    21661 accgcatcta tatcgccgtt ttggtcgata aggaaagcca aaaggcgatt ttaatcggca
    21721 aaggcgggga gcgtttgaag aaaatttcca ccgaagcgcg gttggatatg gaaaaactgt
    21781 ttgataccaa agtatttttg aaggtctggg tcaaagtcaa atccggttgg gcggacgaca
    21841 tccgcttcct gcgcgagctg ggtttgtagt ttttcttgct gaactttacg caaatgccgt
    21901 ccgaacaggt ttcagacggc attttgtttc aatcgggaat atctttgtta aaaacgggtt
    21961 gatattatct gtgca
//
LOCUS       DAWKQB010000017        21045 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.017, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000017 DAWKQB010000000
VERSION     DAWKQB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21045
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.017"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(68..814)
                     /locus_tag="WJL14_000502"
     CDS             complement(68..814)
                     /locus_tag="WJL14_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ7655620.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(993..1664)
                     /locus_tag="WJL14_000503"
     CDS             complement(993..1664)
                     /locus_tag="WJL14_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ7655621.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJL14_000504"
     CDS             complement(1733..2404)
                     /gene="rpiA"
                     /locus_tag="WJL14_000504"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ7655622.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDQPLTMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2481..2963)
                     /locus_tag="WJL14_000505"
     CDS             complement(2481..2963)
                     /locus_tag="WJL14_000505"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ7655623.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIESQAAVLLVRI
                     "
     gene            complement(2995..3684)
                     /locus_tag="WJL14_000506"
     CDS             complement(2995..3684)
                     /locus_tag="WJL14_000506"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ7655624.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKCADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJL14_000507"
     CDS             complement(3681..4415)
                     /gene="dnaQ"
                     /locus_tag="WJL14_000507"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ7655625.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(4571..5881)
                     /locus_tag="WJL14_000508"
     CDS             complement(4571..5881)
                     /locus_tag="WJL14_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7655626.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWAKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLRLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJL14_000509"
     CDS             complement(5871..6056)
                     /gene="ccoS"
                     /locus_tag="WJL14_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ7655627.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6056..6355)
                     /locus_tag="WJL14_000510"
     CDS             complement(6056..6355)
                     /locus_tag="WJL14_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655628.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            6706..7902
                     /locus_tag="WJL14_000511"
     CDS             6706..7902
                     /locus_tag="WJL14_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7655629.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQTTEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8311..10152)
                     /locus_tag="WJL14_000512"
     CDS             complement(8311..10152)
                     /locus_tag="WJL14_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ7655630.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLSLTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10243..10713
                     /locus_tag="WJL14_000513"
     CDS             10243..10713
                     /locus_tag="WJL14_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ7655631.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(10784..11596)
                     /gene="hpnC"
                     /locus_tag="WJL14_000514"
     CDS             complement(10784..11596)
                     /gene="hpnC"
                     /locus_tag="WJL14_000514"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ7655632.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(11683..12165)
                     /locus_tag="WJL14_000515"
     CDS             complement(11683..12165)
                     /locus_tag="WJL14_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7655633.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            12638..12844
                     /locus_tag="WJL14_000516"
     CDS             12638..12844
                     /locus_tag="WJL14_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655634.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPAAEAAATEAPAAEAPAAEAAK"
     gene            12954..14321
                     /locus_tag="WJL14_000517"
     CDS             12954..14321
                     /locus_tag="WJL14_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7655635.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(14502..16472)
                     /locus_tag="WJL14_000518"
     CDS             complement(14502..16472)
                     /locus_tag="WJL14_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ7655636.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDKTKLTDDEITRLKKLRDRNSEY
                     WKEETYHIKSNNRVYPNIPALFPKHPFDPFENINNSKRISFYDKEYTEDYLVGFAQGL
                     GVAKRNGETEKPIRQYFKECLNTGKYSDDTCKSQQSIPTVRSDIFALNTKIKNSHINS
                     EILSVGNYIEWLRPTLNQLSSSQEHLYSDVDPFHYIEVTDNSHVIGQTISLDEFRLEN
                     SLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYGLKDKVPETPVL
                     TFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYTLSLQQ
                     RPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLRVIDGH
                     SNQWVQGKTAPVESNRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNPDTDNL
                     TTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSKGITAS
                     IEAGYNALLAEHFTKKGNRVRFYLQPQAQLTYLGVNGKFSDSENAHVNLLGSRQLQSR
                     VGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEMDGERRMINNKTAIESQLGVAVK
                     IKSHLTLQATFNRQTGKHHQAKQGALNLQWTF"
     gene            complement(16891..17337)
                     /gene="smpB"
                     /locus_tag="WJL14_000519"
     CDS             complement(16891..17337)
                     /gene="smpB"
                     /locus_tag="WJL14_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ7655637.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(17392..18402)
                     /gene="waaF"
                     /locus_tag="WJL14_000520"
     CDS             complement(17392..18402)
                     /gene="waaF"
                     /locus_tag="WJL14_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ7655638.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHQSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINRLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(18533..18608)
                     /locus_tag="WJL14_000521"
     tRNA            complement(18533..18608)
                     /locus_tag="WJL14_000521"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18573..18575),aa:Asn,seq:gtt)
     gene            complement(18799..19551)
                     /locus_tag="WJL14_000522"
     CDS             complement(18799..19551)
                     /locus_tag="WJL14_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ7655639.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMSHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPNAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            19862..20386
                     /locus_tag="WJL14_000523"
     CDS             19862..20386
                     /locus_tag="WJL14_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ7655640.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQILSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     ATSLQLSRCASIADDQSAAISAVISLQICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSVSTAAA"
BASE COUNT         5253 a         6041 c         4829 g         4922 t
ORIGIN      
        1 ataaatttac cacaaaaaag ctgcctcaaa tgaatacccg ggcagctttt tgttgatatg
       61 actccaatca gcggtgttgc ggattgtaac gtttttccaa acgcaggaat atccagccta
      121 agaaagtcgt catcaacaga taaatcaggg cgacggtgta aagcggttct tcataaaccg
      181 aataccggcc cgtaatcgta ttctgaacat acgccaactc cgccacagca atgaccgaca
      241 gcagcgagct gtctttcaag agcgtgatga actcgctcgc caaaggcggc agcatacggc
      301 gcaatgcctg cggcagaatc acatagcgca tcgcctgcgg ataggtcagc cccaaagaac
      361 gcgccgcctc catctgtcct ttgtctatag actggatgcc cgcgcggaaa atctcacaga
      421 tatacgcccc cgagttggcg atcagtgcca aagaaccggc aatcagcggc ccgtatccgc
      481 gacgcagcgc gattgccgcc tcgccgctaa ccaaaatgcc gtctgaagga tggacgaaaa
      541 acggaaacca cacatacgcc caaatcacaa tctgcacaaa cagcggcgta ccccggaaca
      601 gcgtaacata cagcagcgaa accttacgca acgcccacgc cagcacgcgc atcggcgcac
      661 cggctttttc caagtgaatc aggcgcgcca acgccaacaa cagacccaat accgaaccgc
      721 ccgccgtcgc cacgaccgtc agccccaagg tcgtcagtgc gccgtaaaga aacatccagc
      781 ggtattcgta aataatgtca aaacgaaaat ccataaaccg tccgtatcaa aaaccggcgg
      841 aactgccgcc gttgcaaaat aatccgccat tttaccgtaa aaaccgccgc ctgaactttt
      901 ttatcgcggc agacggcggt tgcgcgtctc cgcaaaaatg cagggcgcgc ggttttcaga
      961 cggcatttgc cgttcaaagc cgtgcggtgt ctttaccaaa tgcccaacca ttcgcccacg
     1021 gcatccatcc aatccttatt gcccccgccg ctcctgcctg cccggcggta cgcccacggc
     1081 gcttgcggat ttttagcttt ccacaatcct ttgcgttccc tttccgcctg aatttgagcg
     1141 tcggcataat cggcaaaatc cgccttatcc tgctgttctt tagcataact tttataatgc
     1201 cacgccgccc cgtcctgcac ctgcatcagg ttcaaatcgg ttttgccgac agaaacctgc
     1261 gccacttcgc gctggtagcg gtcggtgtcg aacacgcgga cgctgacttt cctgccttcc
     1321 gccgccgcgc gcaggttgtc gcgcgaacgc gtgccgtaag cctgtttcat ctccggcgcg
     1381 tcgatatacg ccatccggat tttgtgtttc gcgccgtcgc cgtcgataac gtgaagggtg
     1441 tcgccgtcat agactttgga caccgtgcct gtgtagcggt ggccggattt cgccgatgct
     1501 cggcggcggg cgggcgcgtc ggaacccgcg tcccctgccg cgccgagtac gtcgagtacg
     1561 gcaaccgccg tccgcaccgc ctcgctgccg taccccgtat aacccaacgc acccaaaagc
     1621 gacaaggcga cgggaagcca tttcatgatt tttttaatct gcatattttt caaatgccga
     1681 tgccgtctga acatatcgga atcggatttc agacggcatc ttaacgtcag gattaccctt
     1741 ggcagggata gatgactttc gcaccctctt ccgtccccaa aatcaacaca tcggcggcat
     1801 cgcgggcgaa tatgccgttt tcgagcacgc cggtgatttt gttgatttcg tcttccatcg
     1861 tcagcggctg atcgatattc aagccgtgga catcgacgat ttggttgccg taaaacgtgg
     1921 tgtagccgat acgcagttcg ggctgtccgc ccatagcgag cagtttgcgc gaaacaagag
     1981 agcgcgcgct ttcgacgact tccacaggca gagggaattt gcccaaacgt gaaacatatt
     2041 tgctttcatc cgcaatgcag acgaattttt cggatgcgct ggcgacgatt ttttcgttga
     2101 ggtgtgcgcc gccgccgcct ttaatcattt gcagggcgtg gttcacttca tccgcaccgt
     2161 caatatagac cgccaacccc gatacttcgt tcagggaaac gacgggaata tcatattggg
     2221 caagcagttc actggatttt ttggaagtag ataccgcgcc tttgattttt ttgccgctct
     2281 tacccaaggc ttcgatgaaa aagttgatgg tcgaaccggt accgatgccg atgtattcgt
     2341 tttcgggtac gaattcgact gctttttcgg cggcgatgcg cttgagttcg tcttgtgtcg
     2401 tcatattttt gtcctttggg aaaccgtatc aacaaacagc cgccatctta acattttttt
     2461 gcacgtcctg cccgccgcgt tcaaatcctt accagcaata ccgccgcctg cgactctatg
     2521 ccttccatgc gcccgagata accgagtttt tcgttggttt tgcctttgat gttgacgcac
     2581 gaaatgtcta tgcccaaatc ggcggcgatg ttggcacgca tttgcggaat atgcggcgcg
     2641 agtttgggtt tctgtgcaat cacggtcgta tcgacattga ccgcctgcca accctgcgcc
     2701 tgaacgcttt gatacgccgc acgcaaaagg acgcggctgt ccgcatcttt gaactctgcg
     2761 gcggtgtcgg ggaaatggct gccgatatcg cccaaacctg ccgcaccgag cagcgcgtcg
     2821 gtaacggcgt gcagcagcgc atcggcatcg gagtgtccga gcagcccttt ttcaaatggg
     2881 atttcaactc cgccaagtat cagctttctg ccttcggtca gttggtggac atcgtagccc
     2941 tgtccgatac ggatgttcgt catcgtttgt gttcctgatg gtttgaattg aagttcagac
     3001 ggcatcgagc agcagcctga cgatgtatgc gtcctgcggc tgcgtcagtt tcaaattgcg
     3061 cgcgtcgccc tgcaccagca aagggcggat gcccaatttt tccacggcgg acgcttcatc
     3121 ggtaatgccg tccaagtttt ccgcagccaa tgcgcggtgc agcagcccgg cgcggaaaag
     3181 ctgcggcgtt tgcgcctgcc aaaggctcgt ccgctcgacg gttgcactaa tgttcccacc
     3241 gtccgcgcac ttgagcgtat cggcaacggg aattgccaaa atcccacctt cggcagcgtt
     3301 gcccgcctgt tctatcaacc gcgtcaaagc ttcagacggc aggcagcaac gcgcggcatc
     3361 gtgtaccaga atattgtcgg tttccgccgc caaaccggtt tccaacaatt ttgccacacc
     3421 gttgcggaca gtttcggcgc gggtctgtcc gccgtttttc cacacccgaa cctgtggaaa
     3481 tgccgtctga accttatcgg caaacgtgtc ttcgggcgag acgacaacga cggtcaaatc
     3541 gacggcctca tgccgttcaa aaatcccaat cgtatgttct aaaacggttt tgcttccgat
     3601 ttcgacatat tgcttgggtt tgtccgcacc gaaacgcgcc ccgatgccgg cggcgggaat
     3661 cagcgcgata tttttgcgct tcatgcgtcc gtcccgccgt tttcagacgg cacggcttcc
     3721 ttgcgccaga tacaggcttc gcccaagccg tccaaatatt gcccgtgcgc cgccagttcg
     3781 gcttcatccg ccttaatcac tttcagtttg gcaccgagtt tcatttcggc agatgcaaca
     3841 ggtttggctg ccggctcttc ctcctcggcc tcccccatca ggtcgaactg ccgccgcgtc
     3901 atagcaagat agacttcgcc caaaagttcg cagtcgatca atgcgccgtg caggacgcgc
     3961 ttgctgcggt cgacggaaaa acggttgcac agggcatcta gactggcttt ctgcccgggg
     4021 aacatttcgc gcgccatcgc cagggtatcg gtaacggtac agccgagttc ctcaacggtc
     4081 ggcaacccca tccggcggaa ctccatattg aggaagccca cgtcgaattt ggcattgtgg
     4141 ataatcagtt ccgcaccgcg caggaaatcg gcaatctgcc tgccgacctc tgcaaacggc
     4201 ggcgcgtttt tcccttccaa aacctctatc gtcagaccgt gcaccctcgc cgcctcttcg
     4261 ggcatatcgc gctcggggtg gacatagagg tgcaggtttt tgtcggtcat ttggcggttg
     4321 accatttcca gaccggcaaa ctcgaccaag cggtcgccgc cgtcggcata cagaccggtg
     4381 gtttcggtat cgaggatgat ttggcgtgtc gtcatatcgg tgtctttctt ctatcttcgt
     4441 aaattgctta ttttttaagc aatgtatttt tctgttttca tttcaatgca caaacccact
     4501 tattcacagt gtgttcacaa cattgggcag gcggattgtg tattttgggg acaatttttt
     4561 cagacggcat tcaaggtttt ttcctgattg ccgccgcgcc taaaaaccgc ctttcgcgct
     4621 taatcaaaaa taccgacaac ggaatattgc ccaaagcgac aatcagatac aacaaggaaa
     4681 tgctgtcaaa caaaaacagc aacaccgcgc tcaaaacggc agcggaaacc ataaaaatac
     4741 cgttaacgat attgttggcg gcaacggcgc gggcgcggaa agtctcgcta ctggcggttt
     4801 gcagccaggt atagagcgga acggagaaaa atccgccgaa aaagccgatc agcgtcatca
     4861 ccgccatcac gggatatgcc catccttgcg ataaaaacca aaaaatgccg ttcagccctt
     4921 caaaacggtg tccgtgcgtc agccacacca aaaccaagcc gcaaaccgtc aaacccaacg
     4981 caccaaccgt tacccaagcc aacctcagcc gttccctgct gaacttggca cacagtaccg
     5041 aaccggcggc aataccgatg gaaaacaggg caagcatcag gttgaaaaca ttgtcgttgc
     5101 cgcctagatg gatttgggta aaggtcggca gttgcgtggt ataaaccgcg ccgacaaacc
     5161 aaaaccacga aataccgata atggcggtaa aaacgggctt gtgccgcacc gtttcacgca
     5221 gcagggattt tgtaccccgg acaatattcc actcaatttg tgtatcggca gccttggcgg
     5281 gtacggacgg cataaacagg ctgccgaccg tgcctcctac ggcgaccagc aaaaccagta
     5341 tcccgacaat ataaggcggt acacctgcca ccgcagtccc cagtatctga ccgaacagga
     5401 tggcgacaaa cgtacccgat tcaatcaggc tgttgcccat catcaactct ttgtcgtcga
     5461 gataatcggg caggatggcg tatttcagcg gcccgaacag cgtcgattgc gcgcccatgc
     5521 aaaacagaca cgccaaaagc agcggggcag accggatata aaacccgtat gccgccaccg
     5581 ccataatgat catttccagc accttggccc aacgcgccaa aacggccttg tcgaatttgt
     5641 tacccaactg ccccgacagc gaggaaaaca ggaaatacgg caaaataaac agcaacgcgc
     5701 ccaagttcaa catctgtccg gcaggcagga agccgttttg ccccaaaccg taaaacccaa
     5761 tcatcacaaa cagcgcggtt ttgaacacat tgtcgttgaa cgcgccgaga aactgcgtag
     5821 cgaaaagagg tgcgaaacgg cggcttttaa ccagtcccaa accgcctttt ttagcgtaca
     5881 tcgttttccc tctctttttc aatcagttta cttgtcgaat catcatccat caggatgcgg
     5941 tgcgccggcc cttccaagtc gtcaaactgc ccgtttttgc ccgaccacca aaaaaaccag
     6001 ccgatgacaa acgccaaaat aatgctgatg ggcaccaata taaacatgct ttccatcaca
     6061 tattccctgt caaatcgttc aaaacaaaag tctgccccga cacggtcaga tattcgttac
     6121 gcaaagttcc gacgggagct tcgtcaaaaa acagctcgat acggtctttg accacgcgcc
     6181 aatattgggg gatttccgtc tgaccgaacg gcgacaggac atgattttcc attccgcctt
     6241 caagtttgac ggcaaaacgc ccgctttgcg gccgtgcttc cgattcgtcg tcggcaagca
     6301 ggatgaaaaa gcctatatgc cgtcccgatt ggtcatgaat actgaaataa tgcataaatt
     6361 tcccacccgc cttttttcag acgacaccaa ctaaaaacag ggcgaatgta ccagtttgga
     6421 cgggaagaat gcaaagaaat tctccctccc ccagccgaaa acaccggcaa accgcatatc
     6481 cccctttttt ccgtcaaaat gcccgacttc cgccattttc acgcaaacgc ccgattaagc
     6541 caagcaattg caaagatttt ttgctagaat agcctgcttc ttttatcaac cttttcagac
     6601 ggccccacta ctttcccgcc caggaaggca aaacggattc ggcacgaatc cggttagtat
     6661 ccgtgtccga ttccaatgcc gtctgaaact ttccggagta agaaaatgtc ccaaaaattg
     6721 atcttggttt tgaactgcgg cagctcgtcc ctcaaaggcg cggtcctgga taacggcagc
     6781 ggcgaagtcc tgctcagctg ccttgccgaa aaactcaacc tgcccgatgc ctacatcaca
     6841 ttcaaagtaa acggcgaaaa acacaaagtc gatctgtccg cacatcccga ccacaccggc
     6901 gcggtcgaag ccctgatgga agaactcaaa gcccacggcc tcgacagccg catcggcgcc
     6961 atcggccacc gcgtcgtcag cggcggcgaa ctgtacagcg aatccatcct cgttgacgac
     7021 gaagtcattg ccggcatcga aaaatgcatc ccgctcgccc ccctgcacaa ccccgcccac
     7081 ctcttgggcc tgcgtgccgc gcaaagcatt ttcaaagacc tgcccaacgt cgtcgtattc
     7141 gatacctcct tccaccaaac catgcccgaa gtcgcctaca aatacgccgt tccgcaggag
     7201 ttgtatgaaa aatacggcct gcgccgttac ggcgcgcacg gtaccagcta ccgcttcgtc
     7261 gccgacgaaa ccgcgcgctt cctcggcaaa gacaaaaaag acctgcgtat ggtcattgcc
     7321 cacttgggca acggcgcgtc cattaccgcc gtcgccaacg gcgaatcgcg cgacaccagt
     7381 atgggcctga ccccgctgga agggctggta atgggtacgc gcagcggcga catcgatcct
     7441 tccgtattcg gcttcctcgc cgaaaacgcc aatatgacca tcgcccaaac cactgaaatg
     7501 ctgaacaaaa aatccggtct gctcggcatt tccggcctgt ccaacgactg ccgcaccatt
     7561 gaagaagaag ccgccaaggg gcataaaggc gcgaaattgg ccttggatat gtttatctac
     7621 cgccttgcca aatacatcgg cagtatggcg gttgccgcag gcggtttgga cgcactggtc
     7681 tttaccggcg gcatcggcga aaactccgac atcatccgcg aacgcgtgat cggctacttg
     7741 ggcttcctcg gtctgaacat cgaccaagaa gccaacctga aagcccgctt cggcaacgcc
     7801 ggcgtgatta ccactgccga cagcaaagcc gttgccgtgg tcattccgac caacgaagag
     7861 ctgatgattg cccacgacac tgcccgtttg agcggtctgt aaggttttat ccgcacacga
     7921 actgcctccg gaaatggagg cagtttttta tccggctttc catgcttaaa cagcactgcc
     7981 tcttttcaga cattgacggt tgcagccgac tacctgaacc ttaatgattg actatttttt
     8041 aatcatgtta ttattttcca taaaatacat gacattaaga tgtttttcca caaaagatac
     8101 acacaccggc aaacaccggc tgtgtttatc ttttcttatg cctatttttt aatcatcgta
     8161 tttttatctt ttaatttcaa tacgcaaact aacttataca cacggttttc acatctttag
     8221 actgcttccg tgtgtatagt ggatattgcc gttttccttt ctgacaaaaa tgccgtctga
     8281 gaacttcaga cggcatttga aacatcggaa tcagcggttt tgttcatacc actcgataaa
     8341 cttgtctgct ttgacaaaac ccagcagcgg ctcgctgcgg ctgccgtcgg agcggacgac
     8401 aaacacgccc ggcggcccga acagaccgta ttctttcaac aacgcctgat gttcgggcgt
     8461 gttggcggtt acgtcgattt ggaaaaagcg ttccatatcg actgcctgat gcacttccgg
     8521 ctgattgagc gtgtaagccg ccatttcttt gcaggaaatg caccagtcgg cataaaaatc
     8581 caaaacgacg ggtttgtcgg gatgttcttt caacgccgta tccatcgctg ccttcagcgc
     8641 ggcagtatcg gcaaacattt ttccgtgttc cgaagatttg cctgcttcgg ccggcggatt
     8701 gagggtcagg aaatggtgca gcgcggtcgt tttgccgttt gcgccctgcc agccgaacca
     8761 cgcgccgcct atcagcaata tgccgcccaa tgcgaatgcc acagctttcg gacggcgttt
     8821 ctgcctgcgt ccgttgacca gcagcataca ggcaggaacc agcatcagca gcgtgtacag
     8881 cgcgacgacg agataatagg gcaagtgcgg cgtggcgagg taaacggcga cggcaagcag
     8941 gataaagccg aaagcgtatt tgacggcatt catccaatcg cctgccctag gcaggatatg
     9001 cccgccgaac gtgccgatgg caatcagcgg aacgccagtg cccaacgcca aagtgtaaag
     9061 tgccaaaccg cctaaaaccg catcgcccgt ctgaccgatg tagcccaaag caaatgccag
     9121 cggcggggcg acacacggcc cgacaatcag cgcggacaat atgcccataa taaagacgga
     9181 aacgatttta ccgcctgaaa gcctgctgct ttgattctga aaatacgact gcacggcgtt
     9241 gggaagctgg atgttgaaca gcccgaacat agacagtgcc aagacgacca ttaaagccga
     9301 tgccgccaac accacccaag cctgctgcaa ccatacggtc agcagcgcgc ccgtcagtcc
     9361 ggcaacaatg ccgaccagcg tataagtcag agacaaaccc tgaacataaa cgacggacag
     9421 cacaaacgcc cgcgccttgc ccgccttttt gtcaccgacc acaatactgg aaacaatcgg
     9481 caacagggga tacatacagg cggtaaaact cagacccaaa ccggcgagaa aaaacgccaa
     9541 aagattggcg ttgagcgtat cccaagacag cttgaaacgg ctgtcgccgc cctcatcccc
     9601 cttcgggggc ggcagcgccc cgctgccgtt ttgagaggaa ggctgcaaaa agcggtcttt
     9661 ggcggatgcc ggttcgtcgg tttgcggatg gtaagtgccg ttgccggaaa tatcaaactc
     9721 ggtatccacg ggcggatagc acacgccggc ttcggcacag ccctgatagg tcaaaaccaa
     9781 tttatacggt tcgccgacag cctttgcata aggaaaggca acctgcgctt cgtgatggta
     9841 aaccgtctgc ctgccgaaaa actcgtcttc cttctcttcg cccttgctga aagaaggctg
     9901 tcccaacaaa tccgccggat cggtcttgcc gacgattttc gcctgataca tatagtatcc
     9961 gtcggcaatc ctgaaacgga cgttcacacc gtcgtcggca acggcaagct ccggcacgaa
    10021 tgccttttcc ggcggcagca gatcgttcgc atccagcgcg aaagctcgtc cgcacaacat
    10081 caaaaatacg gcgaacaggc aaatcagttt tttcataatc gaatccgttt cagacaaata
    10141 atttgtctgc attataaatg gtaaggttga cggtgggatt taatttatgt aaaacccgcc
    10201 attatccgaa cctatttcca taaacatctt atcgaacccg ccatgtacga tgtcaatacc
    10261 cacgatgtcc gccgcttttt cgcccgcgtg tggcagcagc ggctcaatcc gctgcaactg
    10321 agcgcactgg aacagaaagc cctccgcatt atcgaagccc atcccgaata ccaccgttat
    10381 ctcgaacgca tcgaagacca tctggacacc gactggctac ccgaaaacgg cgaaagcaac
    10441 cccttcctgc atatgtcgct gcatttgtcc gtccaagaac aggcgggcat agaccagccg
    10501 cacggcatac gcgcaatcca cgacaccctg tgcgccaaac gcggctggct ggaagccgaa
    10561 cacgaaatga tggaggcact ggcggaaaca ctgtggacgg cgcaacgcta cggcaccggt
    10621 ttggatgtca atttctacat gacccgactg cgcaaactca tcggcttggg tgcagaagat
    10681 caagccagat tgaacccgca tgaaatcgcc tgaccatacc aaccgcctgc aaaatgccgt
    10741 ctgaagcgga acaacccctt tcagacggca tgcatttccc ccatcatttc cacaacgcgc
    10801 gtttcagcat aatcaaccaa tccttcttat ccaaaacggg gcgttgtgca aacacatcgt
    10861 atcggcacgc gtccagtttc tgcaaaatca actgcgcccc caacacaatc atacggagtt
    10921 ccaaaccgat acgccctttc aattcgcgcg ccaaaggcga acccgccttc agcatacgaa
    10981 atgcgcgccg gcactcatac gccatcagcc gctgaaacgc cgcatccgcc ctaccctccg
    11041 caatctgtcc ttcggaaaca ccgaatttca acaaatcgtc ctgcggaata taaaccctgc
    11101 ccttttgcca atccacagcc acatcctgcc aaaaattcac cagttgcaaa gccgtacaga
    11161 tgccgtcgct ttgcgccacg cacaccgcat ccgttttccc gtacaaagcc agcataatgc
    11221 gtccgacagg gttggcggaa cgccggcagt aatcggccag ttcgccgaaa tgcgcgtacc
    11281 gcgttttaac cacgtcctgc gaaaacgccg aaagcaaatc ataaaacggc tgcaaatcca
    11341 aaccgaacgg cacaaccgcc tcggcatcca atcgtgcaat caaaggatgc gccgaccggc
    11401 cgcccgatgc caacacgtcc aactcgcgcc gcaaagcatc caaccccgcc aacctggctt
    11461 cagacggcat actgccctcg tccgccatat cgtccgccgt ccgtgcaaac gcatacaccg
    11521 catgaaccgg cttcctcaac ctgcgcggca aaatcagcga accgacggga aaattttcat
    11581 aatgcccaac cgacatacct tctccatcca tcaaacaaaa tgccgtctga aatggaacaa
    11641 acccttttca gacggcatca gatacctcca agctgccggc aatcagtggt ggtgatgacc
    11701 gtgcggaccg tggacatgac cgtgtgcgat ttcctcatcg gatgcatcgc gcacactttc
    11761 aaccgtagcc ttaaagcgga ttttcatgcc tgccaaagga tggttgccgt ccaccaccgc
    11821 cttaccgtcg gcaacatcgg ttacacgata gacgacaaca tcgccggttt caggatcgtc
    11881 ggcttcaaac atcatgccga cttcgacttc aacagggaac acgcccgcat cttcaatacg
    11941 gaccaactcc ggatcctgct cgccgaacgc atcgtcgggc gaaagcgcca catcgaccgt
    12001 atcgccggca tccttaccgt gcaacgcctc ttccaccaaa gggaaaatgc cgtcgtaacc
    12061 gccgtgcaga tacgcaatcg gttcttcggt tttgtccaaa agctgattgt tggcatcata
    12121 catctcataa tgcagcgaaa ccacggaatt cttcacgata gccatatttg tcctttcagg
    12181 aacagcagat taattacagg cgcattctaa cacaaccgcc gcgccggccg attaccgtta
    12241 acctgttcat aaactgtaca gcacatattt caatgtaaat ctttgttatt ttattgcggt
    12301 gtaacttttt tacaacattc ttaaaaccat tccgacctgt ctgccgactt tcccaatccg
    12361 ccttaataaa tcatacaaga tactgaaatt atattaatct ctataatatt tatccctatc
    12421 gaatttttaa cagcaaaacc gttttacagg atttatcaat ccgcccgcca gaaaactttt
    12481 cattcaaacc tttttcccat ctgtacgaca ttgcaatccc ttattccata gtgcataatt
    12541 acgcaaattc agcgatgaat ttccaacccg gtttgtagta tggtcgataa agacctattt
    12601 gtttcaataa tttaaattgg ttctaaaggt tactaaaatg aaaaaatccc tgtttgccgc
    12661 tgctttgttg tctttggttt tggcagcctg cggcggtgaa aaagccgctg aagctcccgc
    12721 tgctgaagca cctgccgccg aagctcctgc tactgaagca cctgccgccg aagctcccgc
    12781 tgctgaagct gccgctaccg aagcacctgc cgctgaagct cctgctgccg aagctgcaaa
    12841 ataagcattt tccgcttgca aaaaagcagg atacgttcag tatcctgctt ttttgatttt
    12901 tcagacggca tcagattccc ttcctcaatc ttctccctac ccttccgaca aacatgcctg
    12961 accttcatac cgaattttcc cgactcctgc cggcagatga aattgccgaa ctttctccga
    13021 cacttttaaa agaccagcgc aaccgcttta cgtctgcacc agacatcatt ttgcagccgc
    13081 gcagcgttga aagcgtgcaa accattatgc gtttttgtta cgaacaccgc attccggtta
    13141 cgccgcaagg cggcaatacc ggtttgtgcg gcgcggcagt atcggaaaac ggcgtattgc
    13201 tgaacctttc caaactcaac cgcatccgca gcatcaattt gtcagacaac tgcataaccg
    13261 tcgaagcagg ttccgtactc caaaccgtcc aacaggcagc cgaagcctca aacaggctgt
    13321 ttccactcag tctcgccagc gaaggctcgt gccaaatcgg cggcaacatc gcctgcaatg
    13381 ccggaggttt gaacgtattg cgttacggca cgatgcgcga cctggttatc ggtttggaag
    13441 tcgtcctccc caacggcgag ctggtttccc atctccatcc cctgcataaa aacaccaccg
    13501 gctacgacct gcgccatctg tttatcggca gcgaaggcac actgggcatc atcactgccg
    13561 ccacgctcaa gctttttgcc cgtccctcgg acaaggcaac ggcatgggtc ggcatacccg
    13621 acatcgaatc cgccgtccgc ctgctgaccg aaacccaagc tcactttgcc gaacgcctat
    13681 gcagttttga gctgatcggc cgttttgccg ccgagttgtc ttccgaattc agcaaattcc
    13741 ccctgccgac acattcagaa tggcatattt tacttgagtt gaccgactca ttacccgaca
    13801 gcaatcttga tgatcggctt gtcgaatttc tttataaaaa aggatttacc gacagcgtat
    13861 tggcacaaag cgaacaagaa cgtatccata tgtgggcatt gcgcgaaaac atctccgcat
    13921 cgcaacgcaa actgggcacc agcatcaaac acgatattgc cattcctatc ggacgcgttg
    13981 ccgactttgt ccgccggtgc gccaaagatt tggaacagaa ttttaaaggc atacaaatcg
    14041 tctgcttcgg acatcttggc gacggcagcc tgcactacaa tactttcctg cccgaaatcc
    14101 tcagcaatga agtctatcgt tacgaaaacg acatcaacga cgcagtttat cgcaacgttc
    14161 ttgcctgcaa cggcacgatt gccgccgaac acggcatagg tatcatcaaa aaacagtggc
    14221 tggacaaagt acgcacgcct gccgaaatcg ccctgatgaa aagcatcaaa caacaccttg
    14281 atccatataa cattatgaat ccgggcaaac tgcttccgta accggcattt ctgatttgca
    14341 tacacaacaa agaaagggac aatagatccg attgtcggtt tagcgcgagc tcgtgagtgc
    14401 ggtaaaaaat tggtggaaat gacacgaaaa atgaccgcat ttttaaaata aaaaaatcgg
    14461 cagtaaattt ccctgccgat tttattttgt tacaacttaa cttaaaacgt ccactgcaaa
    14521 ttcaacgcac cttgtttagc ttgatgatgt ttgcctgttt ggcggttgaa tgtggcttgt
    14581 aaggttaagt gagatttgat tttcactgct acacctaatt ggctttcaat tgccgtctta
    14641 ttgtttatca ttcgacgctc tccgtccatt tccacaccga aaggtttgtt gtggtaaagc
    14701 gcgttcacag cggcgaaagg ttcaatagcg atatttttat agagtgaaaa ttgcgcttta
    14761 gcttgaacgc ccactcggct ttgtaattgg cgggagccaa gtaaattcac gtgggcattt
    14821 tcgctatcgc tgaattttcc gtttaccccc aaataagtca attgcgcctg tggttgcagg
    14881 taaaaacgaa cacggttgcc ctttttagtg aagtgttccg ccaataaagc attgtaaccc
    14941 gcttcaattg aggcggtaat accttttgaa gtaaaacgtt ctgtagcatc ttcagtgtta
    15001 atgcggtggc ggaagcgttg atattgtacc cagctatccg catacgcacc tgtctgtttg
    15061 tcctgaagct gatgccaagt ggcataaatg cccgccccaa agcctttcac atttcccgtt
    15121 gtaagattgt ctgtatctgg attgcggaaa gtgctgcgtt gttctgcttg tccgctcatt
    15181 aagccaacgg aaagttgatt actttcgttt tgccatgtga atacttcgcc gccgagttgc
    15241 acacctttac gattgctttc tacaggtgct gttttgcctt gcacccattg gttggaatgt
    15301 ccgtcaatca cacgcaacca caagcctttg cgtggtaaag tgcggtcgaa aatatcgctg
    15361 tttttgtcgt tcaaacgcaa ggcgaataag gtattggcag cttgagcctg ttgtgcataa
    15421 atcgaaatat catcgcgttc ttgcactttg gtaaaaaagc cttctgggcg ttgttgtaaa
    15481 gaaagcgtat aaattccctt ttggtgtttg ccagaaagac ggaatgcgtg tttatctgct
    15541 gtgccattta ctttgataat ttgatgcccg tcgagacttt ttaaatcgtc tattggattt
    15601 tcgaagatga tgtcggaagt gccagttata tttttttcaa aagttaaaac tggtgtttca
    15661 gggactttat cttttagacc gtaagcaaaa cgaaaacgag ctccgccagc ataatcttct
    15721 tttacgagta aactttcact tttagtatta aaacggatgt ctgcattcgt tgtttttaat
    15781 tccccaacat cagaatccca acggggctcc cagagagaat tttctaagcg gaattcatcc
    15841 aaactaatcg tttgcccgat aacgtgcgag ttgtctgtaa cctcaatata gtggaatgga
    15901 tctacatcag aatagagatg ctcttgcgaa ctagaaagct gattaagtgt tgggcgaagc
    15961 cattcgatat aattacctac agataaaatt tcgctgttga tatggctatt ttttattttc
    16021 gtatttaggg cgaaaatatc acttcttact gtaggaatag attgttgaga tttgcaagta
    16081 tcatcactat atttcccagt gtttaaacat tccttaaaat attgccgtat tggtttttct
    16141 gtttccccat ttctttttgc aacgcctaaa ccttgagcaa aaccaacaag gtaatcttca
    16201 gtgtattctt tgtcataaaa agaaatcctt tttgaattat tgatgttttc gaatggatcg
    16261 aaaggatgtt tagggaataa tgcgggaatg tttggataaa ctcggttgtt actttttatg
    16321 tgataagttt cttctttcca atattcacta tttctatcgc ggagtttttt taagcgggta
    16381 atttcatcat cagtgagctt ggttttgtcg taaacataat caacagccaa aagaggagag
    16441 gtataaagaa tagaaaaaaa tagacttata ataaatgatt ttttaaactt ctgcttgctt
    16501 gcttgcttcg agtttcataa taaattttcc tttgtcaagt aaagataaat ggggcgtgga
    16561 ttttagcata gaattgaaca aaaaatgtca tttagctcac atttttctct atttatttct
    16621 tatttattaa aagtaaacgt ttgctttttg ctattttgtc aagccagttt gaaaatgtgt
    16681 ataattgccc tcgttattta caaaaatttc aggaaaaatg accgcacttt acccttggct
    16741 aatgccaatt tatcatcaaa ttgctcaaac ctttgacgaa ggtttggggc atcatgccgt
    16801 actgattaaa gcggatgctg gtttaggtgt agaacgttta cacatcaggc ggcagccttg
    16861 cccataccgt ctgaagcact gtttccacaa tcagcgcgta tgcttaatca accgctgttt
    16921 ctcgcgtttc caatccgcct ctttcatact ctgacgtttg tcgtgctgtt tcttaccttt
    16981 tgccaaaccg atttccatct tgatttttcc gcgcgaaaaa tgcaaatcca gcggcacgat
    17041 ggtgtagccg gcacgttcgg ttttgccgat taatttgttg atttccgact ggttcaacaa
    17101 gagcttgcgc ggacgtacgg catctggttt aatgtgtgtc gaggctgtgg gcaaagccgt
    17161 aatatggcag ccgaccagat aaaacgcgtc ttttttccaa tagatataac tctctttaag
    17221 ctgtacgcgc gcggcgcgga ttgctttgac ttcccagcct tccaagacca aaccggcttc
    17281 aatccggtct tcaatgaaaa aatcgtgaaa tgctttttta ttgttcgcaa tagccataaa
    17341 catcctatca atatccgccg tcagacggca taaacccgaa aacagaaccc atcataccgc
    17401 ctcttcaacc gcctgcacaa tcttctcggg atacagcctg ttgaggcagt cggtatgccc
    17461 cagcggacat tcccgcttaa aacagggcga acattccaag tgcaggctga cgattttcgc
    17521 cctgtcgctc aaaggcggcg tatgcgtcgg gctggaagaa ccgtaaaccg ccaccacctt
    17581 cctgcccaat gccgccgcca agtgcatcag cccgctgtcg ttgcacacga ccgtgtccgc
    17641 caacgacagc aaatccattg cctgcgacaa atcggttttg ccgcacaaat tgacacacat
    17701 accgtctgaa aggcggttga tttcctcggc aatttcatca tctttttgcg aaccgaacaa
    17761 ccaaacctgc caacccgccg ccagataatg tttgcccaac tcggcaaaat gccttgtcgg
    17821 ccaacgcttt gccggcccga attccgcacc cggacaaaaa gccagaacag gctttccaag
    17881 attcaaaccg aaagtttcga cagaaatttc ccgccgccgt tcatcaatgg aaaactcggg
    17941 gaatcccgaa tgcccgtcaa aatcttcctg actctgatgc gcgagagccg tataccggtc
    18001 caccatcaaa ggcagacgtt ccttatccag cctgcgtata tcgttcaaca gaaaataacg
    18061 gctttcaccg acataacccg tccttttacc gatacctgtc gccagcgcga tgattgccga
    18121 tttcaaagaa ccgggcaaca cgataacctg atcgtatccg cgccgcccca aatccctacc
    18181 gacccgccaa cggcgtttca actccaacgc accgtgtccg aacggatttt caagaatctc
    18241 attcacttcc ggcatacgct caaacaccgc catcgaccac ttcggtgcga acacatcaat
    18301 cgtgcaaccg gggtgaagtt ccttcaaacg gcggaacaag ggctgggtca tcacgcagtc
    18361 gcctatccaa ctgggagaaa taatcaggat tttgatggac ataacaagaa accgaaatca
    18421 gacaggcaga attttaccgc gaaaccgttg gaaaacctat cttgccgcat tccgaacgcc
    18481 gtacgtgcaa atatgaaaaa gcccgaacat tcaagttcgg gcttcaaaat tctggctccc
    18541 cgacctgggc tcgaaccagg gacctgcgga ttaacagtcc gtcgctctac cgactgagct
    18601 atcggggaat ggggcgtatt atagcgtccg gaaaaaatgt gtcaatcctt aattttggaa
    18661 aaatgggcga caaaacgaca agcatatgaa tcagaaagac attaagaccg atgccttaaa
    18721 aggattgccg ttgtatgaat ttccacagcc gtcatcacac catatttaag cccgatgagc
    18781 cgttctgccc tcccccgctt aaaacaatgc cgtctgaact tcgccgcgtt ccaaagccag
    18841 taaaaactgt ttgcggttca acccgcccgc gtagccggtc agtttgccgt cgctgccgat
    18901 gacgcggtgg cagggaatca agatagacac cttgttctgc ccgttggcgg cggcaacggc
    18961 gcggacggcg ttggggttgc ccaaacgctg cgcctgctct ttgtagctgc gcgtttcgcc
    19021 gtaaggaatc gccaagagcg cgtcccatgc ctgcttttga aactcggtgc caatctgctc
    19081 caaaggcgtg gcaaaggttt tcagacgacc cttgaagtat aagtccaatt cctgccgcaa
    19141 aagttgcgtc cgctcatcct cccgaaacac aaaccgtccg cgcaaggctt tttggacggc
    19201 ggcaatttcc tgttccaaat gcttctgtcc gacaaactcc agcagacaca atcccttatt
    19261 tccgaacacc gccagcatct cgcccaaagg cgtggcaatg gcggcacaca ccagctcatt
    19321 caaactgtcg ggataacgcg cttccaacag acggatggct cggcggatac ggacatattc
    19381 ttcaggcgcg cagccgatat tgtcccaaaa atcccgatcg aactgcccgg cttcgcattc
    19441 cgtcaaattg ggatgcgaca taacgccgca ctcacaaacc cgggtttcga gccaatagcg
    19501 gatttcatcc cattttgacg gcagattgtt taaggaaggc agggtaatca ttcgtttgct
    19561 ccgtatctct atcatggatt tgacggcaaa atccccaatt ttcgccattc ccgcacgccg
    19621 gtgcaggaac aggctgcggc ggaaaccttg agggtcgggt tgcggcaatg cctaaatatg
    19681 cgatatttcc aaagcatcag ggaaaggaat taaagacttc agacgacctg caaagggtcg
    19741 tctgaaacca cgatttttgc atttgcgcat tccggcacat catccaaccg tttcggcaca
    19801 ttcctgccgc cgttgacagc ctataatgaa tccacttatt catcaagcaa aggaatcatc
    19861 tatgcaaacc cgcatcctct ccgccgtact gctggctttt tcaaccgctg cctttgccgg
    19921 gggcgcattc acgctgcaat tcgacaaccc gtccgaagac ggcggcttca cgcaaaacca
    19981 gattttgagc gcgccttacg gctttggctg ttcgggcggc aatgcttcgc ccgcgctgtc
    20041 gtggaaaaat ccgcccgccg ggacaaaaag tttcgtcctg accgtttacg ataaagacgc
    20101 gccgaccgga ctgggctgga tgcactgggt ggtcgccgac attcccgccg atgtccgccg
    20161 ccgcaatgcg acctcgctgc aattaagccg ctgcgccagc atcgccgacg accagtccgc
    20221 agccatatcg gcagtaatca gtttgcagat ttgccgcatc aggttgacgc cttcgtacac
    20281 ggcaaaaccg atgccgtcat gctgcaacca cgccaacacg ccgcaaagcg cggcctccgc
    20341 cgcattgtgc ggcacttctt catccgtcag caccgccgcc gcataatcaa acgccgcgcc
    20401 catccgcctg gggtgcggca gcttcaccgc atcgcacact gcctgcgccg tcccgtattg
    20461 tgcggcgaac ctttctacgg tttcctgttc aaaagcaatc cattgcgcct gatagaggcc
    20521 gtctgaatcg ggaatattaa taacgtcaaa cgtctgtccg cctgccaacg caaccgcctc
    20581 acccgcaatt ctcgcctcgc ccaccgcagc ttcttgcttc cgcgccgcac gataagcaca
    20641 gctggttcat ataccgccac gctgcggtac aaggcggtat gatgttgcac gatgccgccc
    20701 aaagcaccca atcgttcgcg cgtatgaaag taatttgacg gcactattat ctccaacacg
    20761 cagggccgca cataaggcgc agccctgttt accctgattt ggaaggggtt acttcccaaa
    20821 aataaaccaa aatcccactg ccccgaatgg ccgggtttca accatattcc gtcggtgtgc
    20881 ctgcaacttg actccctccc ctgtggggga gggtcgggga gagggtaaaa cggggcagat
    20941 acaggcaata tttccgttgc cgccccgatg ccctctccct caccctctcc cacgggagag
    21001 ggaatggatt gctgtttaaa taaaaaaatc tacataaaaa tcaat
//
LOCUS       DAWKQB010000018        20933 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.018, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000018 DAWKQB010000000
VERSION     DAWKQB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20933
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.018"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(79..771)
                     /locus_tag="WJL14_000524"
     CDS             complement(79..771)
                     /locus_tag="WJL14_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7655641.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..1634)
                     /locus_tag="WJL14_000525"
     CDS             complement(1077..1634)
                     /locus_tag="WJL14_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655642.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(2033..2875)
                     /locus_tag="WJL14_000526"
     CDS             complement(2033..2875)
                     /locus_tag="WJL14_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7655643.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3114..4034
                     /gene="pyrB"
                     /locus_tag="WJL14_000527"
     CDS             3114..4034
                     /gene="pyrB"
                     /locus_tag="WJL14_000527"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ7655644.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4044..4502
                     /locus_tag="WJL14_000528"
     CDS             4044..4502
                     /locus_tag="WJL14_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ7655645.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDHFKVVQKRHLNLPDEI
                     AEVFRCPNTNCASHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4567..5232
                     /locus_tag="WJL14_000529"
     CDS             4567..5232
                     /locus_tag="WJL14_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655646.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5295..6512)
                     /locus_tag="WJL14_000530"
     CDS             complement(5295..6512)
                     /locus_tag="WJL14_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7655647.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            6654..7199
                     /gene="def"
                     /locus_tag="WJL14_000531"
     CDS             6654..7199
                     /gene="def"
                     /locus_tag="WJL14_000531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ7655648.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7286..8212
                     /locus_tag="WJL14_000532"
     CDS             7286..8212
                     /locus_tag="WJL14_000532"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ7655649.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSKS
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8291..9550
                     /gene="rsmB"
                     /locus_tag="WJL14_000533"
     CDS             8291..9550
                     /gene="rsmB"
                     /locus_tag="WJL14_000533"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ7655650.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIGNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDTILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9516..10115
                     /locus_tag="WJL14_000534"
     CDS             9516..10115
                     /locus_tag="WJL14_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ7655651.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10115..12235
                     /locus_tag="WJL14_000535"
     CDS             10115..12235
                     /locus_tag="WJL14_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ7655652.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIIKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMMAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVETYA"
     gene            12228..13505
                     /locus_tag="WJL14_000536"
     CDS             12228..13505
                     /locus_tag="WJL14_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ7655653.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKYGAAQTETGPVFDKLGNSAAIQEMNREVGAAVKCASPVL
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGITCNVAESQKIAPASFSEDALAALTRYEWPGNFDQLSSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13596..14783
                     /gene="dprA"
                     /locus_tag="WJL14_000537"
     CDS             13596..14783
                     /gene="dprA"
                     /locus_tag="WJL14_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ7655654.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLMRRFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGKSLGGQNIPVVSGMASGIDT
                     AAHQGALEAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAVGGILDKMGFDPIHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14809..15270
                     /locus_tag="WJL14_000538"
     CDS             14809..15270
                     /locus_tag="WJL14_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ7655655.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15342..17648
                     /gene="topA"
                     /locus_tag="WJL14_000539"
     CDS             15342..17648
                     /gene="topA"
                     /locus_tag="WJL14_000539"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ7655656.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVANPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAREYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEESEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPVMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGGHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17721..18416)
                     /locus_tag="WJL14_000540"
     CDS             complement(17721..18416)
                     /locus_tag="WJL14_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655657.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFQ"
     gene            complement(18436..18987)
                     /locus_tag="WJL14_000541"
     CDS             complement(18436..18987)
                     /locus_tag="WJL14_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655658.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19106..19486
                     /locus_tag="WJL14_000542"
     CDS             19106..19486
                     /locus_tag="WJL14_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7655659.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVLQINDVDTNSGEFAIYTAQDASVKLDLLKMEL
                     KHCKEMLEHKDKEIELLRKLTETV"
     gene            19575..20144
                     /gene="rsmD"
                     /locus_tag="WJL14_000543"
     CDS             19575..20144
                     /gene="rsmD"
                     /locus_tag="WJL14_000543"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ7655660.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTAQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRSFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20267..20518
                     /locus_tag="WJL14_000544"
     CDS             20267..20518
                     /locus_tag="WJL14_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ7655661.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEYPETHDELMAKYEKIIQFK"
     gene            20616..20699
                     /locus_tag="WJL14_000545"
     tRNA            20616..20699
                     /locus_tag="WJL14_000545"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20650..20652,aa:Tyr,seq:gta)
     gene            20730..20803
                     /locus_tag="WJL14_000546"
     tRNA            20730..20803
                     /locus_tag="WJL14_000546"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20762..20764,aa:Gly,seq:tcc)
     gene            20813..20887
                     /locus_tag="WJL14_000547"
     tRNA            20813..20887
                     /locus_tag="WJL14_000547"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20845..20847,aa:Thr,seq:ggt)
BASE COUNT         5244 a         5331 c         5690 g         4668 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat tagacatttg
     1621 tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa gtttttttac
     1681 aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat tgatagaata
     1741 aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg agcctgaacc
     1801 accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg tgcaagcgag
     1861 cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa gcaggatagg
     1921 agcggtaacg caaaggtctc gggctttgat ttcgccgtaa accctgctgc cgccttgtcc
     1981 ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg gttcatttgg
     2041 ggtcgcaacg gaagtggtat gcgcagattt caaaaccgtt tttgaaatac aggcggtgcg
     2101 cgtcggcacg gtcgtggttg acgtggacgt tgaggtggat tttggttacc cctgtttccg
     2161 cgccgatttt gcggacttct tccaaaaggc gcgaggcgta gcctttgcgg cggctttgcg
     2221 gcagggtaac gatgtcatcg atgtggatgt ggcggccgct ggcgagggtg caggcttcgc
     2281 ggaagccgca gacggcgacg gcattgtgtt tgccttcttc aaaaataccc agcaggcggt
     2341 agccttgggg gcgttggact ttgttgatct gttcggtaaa gcggttgatg tcggtcaggg
     2401 cggaacgcaa aacgctcaag gctgcaaagg cggtggcggt gtcgtccgcg ccgatttcgc
     2461 gcaaaacgta ggatgcgccc gaggcggtct gttcctgtgc tttctcggcg gcgtgttttt
     2521 cttcgattgc ctgtgccagc atgacgtgtt cgtcggcagg gttgttttgt ccgccctgtt
     2581 cgcgttcttc gagcagggct ttgcagtcga tgacgcgcag gtcgttgtcg gcggcaaagt
     2641 ccatcaggaa gcggaacatt tgggggttgt cggtttccag ttttttatcg acggcgacgc
     2701 agcggatgtt gtccaccagc atggggcgca cccatttcga ataggaaagc ctgtggtctt
     2761 tggtgaacga ggacagaatg ccgcacaggc gttccgccca gtcgctggga cggaaaatct
     2821 tgccggaact cgttgtgccg tggatgacga cttcgtaggg gttgcagact aacatggcgg
     2881 cttcctgaaa agaaatgtct ggcgcgatta taccttatgc ttatgcgggc gtgtttggat
     2941 atgccgtctg aaaagtacgg gattcgtgcg gtaaaacttt gcggcggcaa atgtgcgata
     3001 atacgcgccg tattgccgct tttgcgaagc tgttccgcaa acatacgggc ggcgtggacg
     3061 acgtataacc ggatacccgc ctgacgcggg ttttttacgg aaggggggca aaaatgccta
     3121 atccgcttta cagacagcat atcatctcca tttcggattt gtcgcgcgaa cagttggaat
     3181 gcctgcttca gacggcattg aagctgaagg cgcatccgcg cggcgacctg ttggaaggca
     3241 aacttatcgg ttcgtgcttt ttcgagccgt ccacgcgcac gaggctgtcg tttgaaacgg
     3301 cggtgcagcg tttgggcggc aaggtcatcg gtttctcgga cggcgcgaat accagtgcca
     3361 aaaaaggcga gacgcttgcc gataccgccc gcatcatttc cggatatact gatgctatca
     3421 tccaacgcca ccccaaagac ggcgcggcgc gcgtggcagc ggagttttcg cgcgtccccg
     3481 ttatcaacgc cggcgacggc acgaaccagc accccagtca gacgctgctc gacctggtta
     3541 ccatttatga aacacaggga cgtttggaca agctcaaaat cgccatggcg ggcgacttga
     3601 aatacggacg taccgtgcat tcgctttgtc aggcgttgaa acgctggaat tgtgaatttg
     3661 cctttgtttc gccgcccagc ctagccatgc ccgactatat taccgaagag ttggacgaag
     3721 ccggctgccg ataccgtatc ctcggtagtt tggaagaagc ggcggaatgg gcggatatcc
     3781 tgtatatgac ccgcgtccag cgcgaacgtt tcgacgaaca ggaatttgcc aaaatccaag
     3841 gcaaattcaa cctcgaagcg tctatgctcg cccgcgccaa accgaacctg cgcgtgctgc
     3901 accccctgcc gcgcgtggac gaaatccatc ccgatgtcga tgccacgccg cacgcctatt
     3961 atttcgagca ggcgaccaac ggcgtttatg cgcgtatggc gatattgtcg ctggtgttga
     4021 acgaagaagt gtgaggaacc gatatggaaa ccccgaaact cagtgtcgaa gccattgaaa
     4081 aaggtacggt tatcgaccat attcccgccg gcagggggct gaccatcctg cgccagttca
     4141 aacttttgca ctacggcaac gcggtaaccg tgggcttcaa cctgcccagc aaaacccaag
     4201 gcagcaaaga catcatcaaa atcaaaggcg tgtgcttgga cgacaaagcc gccgaccgcc
     4261 tcgccctgtt cgcccccgaa gcggtggtca acaccatcga ccatttcaag gtcgtgcaga
     4321 agcggcattt gaacctgccc gatgaaattg ccgaagtgtt ccgttgcccg aacaccaatt
     4381 gcgccagcca cggcgagccg gtcaaaagcc ggttttatgt taaaaaacac aacgggcaga
     4441 cgcggctgaa atgccactac tgcgaaaaaa cctacagccg ggattcggtg gcggaagcct
     4501 gacggattcc cttaaaccga gtgggcggca tttcgtctgc cgcttgtttt gccaatctga
     4561 aatggaatga tgatgcacgc ttctgtccaa agccgtttcg caccgatact ttatgttttg
     4621 attttctttg ccggtttttt gaccgcgcaa atctggttca atcagaaagc ctatactgaa
     4681 gagctgcctc cgcttctgtc cgcattgtcc gccgtcgcgc tggtgtggct ggcgtgggcg
     4741 ttcgtgtcgg cgcgttcaaa ggctaaggcg gaaaagttct accgcgaaaa aatgatacag
     4801 aacgaaagca tacaccccgt cctgcacgct tctttgcaac acttggaaca caagccgcaa
     4861 atgctcgccc tgctggtcaa aaaccacggc aaagggatgg cggaacaggt caggttcaag
     4921 gcggaagtgc tgcccgacga cgaagacgcg cgcacgattg ccgccgagtt ggcaaaaatg
     4981 gatatgtttg cattggggac ggacgcggtc gcctcgggcg aaacctatgg acgcgtgttc
     5041 gccgatattt tcgagttgtc ggcggctttg gaagggcgcg cgttcaaagg aatgttgaaa
     5101 ctgacggcgg aatataaaaa catcttcggc gatgcctgcc gttcggaaac ggcgttggag
     5161 ttgggcgcgc tcaatcaggc gttgcaggag atttcaaaaa catcggaaaa gtccaaacgg
     5221 atattttatt gaagatggaa aaatgccgtc tgaaacggaa ggtgtttcag acggcatttt
     5281 tgtcggatga ttaattattc ggagcggttg aagccaaact tcacgcggct gcggccctga
     5341 tccggtatat tgtccaaatc gcgtcccgga ttggcggcgg tgtcgcctac ggaaatatcg
     5401 gagatgtttt ccaaaatgat ggcggacgac aggtgttcgg aggtgcggta aaccattgcc
     5461 aagcccactt cttcggcagg agtggaaatc agctcgacgg tatccctgct tttgaaattg
     5521 ttggagaggt cgacctgcat cgttttcttg cgtttgtaga ggctcaaaac cgtgcctttg
     5581 tccaaaccgt ccgcctcgcc tttgtcgatg gtgatggttt gaaactggcc ggcaatcctt
     5641 gtgccttcaa acacggaaac gattttagcc tgaaccgggc gggacggttc gtgcggcatc
     5701 atgttgaagc ggtcggtgtc ttccggcatt ttcatcaggt agtcgccctg ctgtatttcg
     5761 gaaatggcgg tttcgaccac cagcggctgt atcgaagggg tgcgcagcgg ggtaatcaaa
     5821 ggatgggtgc gggtatggta ttcgttgtct ttcggccgtt ctccagcctg tttcgagcgt
     5881 tgttcgagga cagagtcggt atagtcgagg gagcgcacga tgccgctgaa tgcgacttcc
     5941 tgcccgagga atttacccgt atccggatcg gtgatgtttt tattgattcg gtaggtcagg
     6001 tagcggcccg gctctttcag gcctttggtg taaaccctgg tgcctttggt gtacagcagc
     6061 ctgccttccg ggcccgagag caggcgcggc gcggcagcgg tttctttgcg ggaaacgatt
     6121 tgcgggtgcc gcataaagat gcggtagaag ttgacatcga tggcgggaat accgtatccg
     6181 gacacttcct tatccggact cattttgacg acggggatgc cgtctgtctg ttccaagccg
     6241 aggcgcggtt cgccgtcaac gtggcgcaac accaatacct ggtccggata aatcaggtcg
     6301 ggattgtgga tttgatcccg gttcgcgtcc cacaggcggc cccattgcca cgggctgtac
     6361 aggtatttgc ccgaaatgcc ccacagggtg tcgccctgtt tgaccgtgta gcgttccggc
     6421 gcgttcgggc gcacctccaa atttgccgcc aaagtttgtg ttgagaatgc catacctgcc
     6481 gcgcagagca gggttataat acgacgttgc ataaccgttc cccttatctg ataaatttcg
     6541 gtttgtcttg cttgattggg ttggaaaaag cggcggcagc ccctcgggat gtgccgcgtg
     6601 ataaaaaatg ttccgcattt taacatcgaa ttatccgcac catcacggta attatgaaaa
     6661 acaggcggcg tatccgccga aggaaagaga aaattatggc tttattgaat atcttgcaat
     6721 atcccgacga gcgtctgcac acggtggcaa agcctgtcga acaagttgac gagcgcatcc
     6781 ggaagctgat tgccgatatg tttgaaacga tgtacgaatc gcgcggcatc gggctggcgg
     6841 cgacgcaggt cgatgtgcac gagcgcgtgg tcgtgatgga tttgaccgaa gaccgcagcg
     6901 aaccgcgcgt gttcatcaac cccgtcatcg ttgaaaaaga cggcgaaacc acttacgaag
     6961 agggctgcct gtccgtgccg ggcatttacg acaccgtaac ccgcgccgaa cgcgtcaagg
     7021 tcgaggcttt gaacgaaaaa ggcgaaaagt tcacgctgga ggcggacggc ttgttggcga
     7081 tttgcgtgca gcacgagttg gaccacctga tgggcatcgt gtttgtcgaa cgcctttcac
     7141 aactcaagca ggggcggatt aagaccaagc tgaaaaaacg tcagaaacat acgatttgac
     7201 ccttttgccg tgccgtctga acgctgcaaa gttttcagac ggcacggtct tgtccgacaa
     7261 ccttacgcat acgcaggaat atgctatgaa agtcatcttt gccggcacgc ccgattttgc
     7321 cgccgccgcc ttaaaagccg ttgccgccgc cggttttgaa attccgctgg tgctgaccca
     7381 gcccgaccgt ccgaaagggc gcggtatgca actgactgcc ccgcccgtca aacaagccgc
     7441 gctggaactc ggtttgcgcg tcgaacagcc cgaaaagctg cgcaacaacg cggaagccct
     7501 gcaaatgctc aaagaggtcg aggcagacgt aatggtggtt gccgcctacg gtttgattct
     7561 gccgcaggaa gtgttggata cgccgaaaca cggctgcctc aacatccacg cttcgctgtt
     7621 accccgttgg cgtggcgcgg cgccgattca acgcgcgatt gaagccggcg atgccgagac
     7681 aggcgtgtgt attatgcaga tggacatcgg tttggacacc ggcgatgtgg tcagcgaaca
     7741 ccgctacgcc atccaaccga ccgataccgc caacgaagtc cacgacgcgc tgatggaaat
     7801 cggtgcggcg gcggttgttg ccgatttgca acagcttcaa agcaaaggcc gtctgaacgc
     7861 ggtcaaacag cccgaagaag gtgttactta cgcgcaaaaa ttgagcaaag aagaggcgcg
     7921 tatcgattgg agcaaaagcg cggcggttat cgaacgcaaa atccgcgcct tcaaccccgt
     7981 gcctgccgcg tgggttgagt atcagggcaa gccgatgaaa atccggcgcg ccgaagtggt
     8041 ggcgcaacaa ggcgcggcag gcgaagtgtt gtcctgttcg gcggacggtt tggtcgttgc
     8101 ctgcggcgaa aacgcgctga agattaccga attgcagcct gccggcggca ggcggatgaa
     8161 tatcgcggcg tttgcagcag gacggcatat cgaagcaggg acgaagctgt aaatcccttc
     8221 agacggcatt ccgatccgca aacgggaatg ccgcctgaaa ccatcagtcg aagaaagcga
     8281 atcacataat atgagtatgg cacttgccca aaaacttgcc gccgacagca ttgcggcggt
     8341 tgccgaagga cgcaaccttc aggacgtgtt ggcgcaaatc cgcaccgcgc atcccgacct
     8401 tacggcgcag gaaaacggcg cgttgcagga catcgcctac ggctgccagc gttatttggg
     8461 cagtttgaaa catatgctcg cgcagatgct gaaaaagccg attggcaatc cgcagctcga
     8521 aagtctgctt ttggcggcgt tgtaccaact gcattacacg cgcaacgcgc cgcatgctgt
     8581 ggtcaacgaa gctgttgaaa gcatcgccaa aatcggacgc gggcagtacc gttcgtttgc
     8641 caacgcgatt ttgcgccgct ttttgcgcga acgcgacaag cttgcggctt cctgcaaaaa
     8701 agacgatgtg gcgaaacaca acctgccgct gtggtgggtg gcttacttga aaaaccatta
     8761 tccgaaacac tggcacaaca tcgccgccgc gctgcaatcc catccgccga tgacgttgcg
     8821 cgtcaaccgc cgacacggca acgccgaaag ctatttggaa aaactggctg cggaaggcat
     8881 tgcggcaaag gcgttggacg aatatgcggt tacgttggaa gaagccgtgc cggtgaaccg
     8941 cctgcccggt ttttcagacg gcattgtttc ggtacaggac ttcggcgcgc agcaggcggc
     9001 gtatctcctt aacccgaaag acggcgaacg gattttggac gcgtgcgccg cgccgggcgg
     9061 caagacgggg catatcttgg aactggcgga ttgccgcgtt accgccttgg acattgatgc
     9121 aggccgtctg aagcgtgtgg aggacaatat cgcccgtttg ggctttcaga cggcatcggc
     9181 ggcgtgtgcc gatgcgcggg atttggcggc gtggtatgat ggaaagcctt tcgacacaat
     9241 ccttgccgac gtgccatgca ccgcttccgg cgtggcgcgg cgcaatcccg acgtgaaatg
     9301 gctgcgccgt ccgaccgacg cgctcaaaac cgcccgccag caggaagccc tgctggacgc
     9361 actttggcag acgctgaaac agggcggcag aatgctgctt gccacctgtt ccgtgttcgt
     9421 cgaagaaaac gacgggcaat tgcaaaaatt cctcaaccgc catgccgatg ccgaaccgat
     9481 cgaatcgcgg gtgcttttac cgaacaaaca ccaagatggc ttttattacg cgcttattca
     9541 aaagcattaa acaatggctt gtgctgctgc cgatgctttc cgttttgccg gacgcggcgg
     9601 cggaggggat agatgtgagc cgcgccgaag cgaggataac cgacggcggg cagctttcca
     9661 tcagcagccg cttccaaacc gagctgcccg accagctcca acaggcgttg cgccggggcg
     9721 tgccgctcaa ctttacctta agctggcagc tttccgcccc gataatcgct tcttatcggt
     9781 ttaaattggg gcaactgatt ggcgatgacg acaatattga ctacaaactg agtttccatc
     9841 cgctgaccaa ccgctaccgc gttaccgtcg gcgcgttttc gacagactac gacaccttgg
     9901 atgcggcatt gcgcgcgacc ggcgcggttg ccaactggaa agtcctgaac aaaggcgcgc
     9961 tgtccggtgc ggaagcaggg gaaaccaagg cggaaatccg cctgacgctg tccacttcaa
    10021 aactgcccaa gccttttcaa atcaatgcat tgacttctca aaactggcat ttggattcgg
    10081 gttggaaacc tctaaacatc atcgggaaca aataatgcgc cgttttctac cgatcgcagc
    10141 catatgcgcc gtcgtcctgt tgtacggact gacggcggca accggcagca ccagttcgct
    10201 ggcggattat ttctggtgga ttgttgcgtt cagcgcaatg ctgctgctgg tgttgtccgc
    10261 cgttttggca cgttatgtca tattgctgtt gaaagacagg cgcgacggcg tattcggttc
    10321 gcagattgcc aaacgccttt ccgggatgtt tacgctggtt gccgtactgc ccggcgtgtt
    10381 tctgttcggc gtttccgcac agtttatcaa cggcacgatt aattcgtggt tcggcaacga
    10441 tacccacgag gcgcttgaac gcagcctcaa tttgagcaag tccgcattga atctggcggc
    10501 agacaacgcc cttggcaacg ccatccccgt gcagatagac ctcatcggcg cggcttccct
    10561 gcccggggat atgggcaggg tgctggaaca ttacgccggc agcggttttg cccagcttgc
    10621 cctgtacaat gccgcaagcg gcaaaatcga aaaaagcatc aacccgcaca agctcgatca
    10681 gccgtttcca ggtaaggcgc gttgggaaaa aatccaacag gcgggttcgg tcagggattt
    10741 ggaaagcata ggcggcgtat tgtacgcgca gggctggctg tcggcaggta cgcacaacgg
    10801 gcgcgattac gccttgtttt tccgtcagcc ggttcccaaa ggcgtggcag aggatgccgt
    10861 cttaatcgaa aaggcaaggg cgaaatatgc tgagttgagt tacagcaaaa aaggtttgca
    10921 gacctttttc ctggcaaccc tgctgattgc ctcgctgctg tcgatttttc ttgcactggt
    10981 catggcactg tatttcgccc gccgtttcgt cgaacccgtc ctatcgcttg ccgagggggc
    11041 gaaggcggtg gcgcaaggcg atttcagcca gacgcgcccc gtgttgcgca acgacgagtt
    11101 cggacgcttg accaagttgt tcaaccacat gaccgagcag ctttccatcg ccaaagaagc
    11161 agacgagcgc aaccgccggc gcgaggaagc cgccagacat tatctcgaat gcgtgttgga
    11221 ggggctgacc acgggcgtgg tggtgtttga cgaacaaggc tgtctgaaaa ccttcaacaa
    11281 agcggcggaa cagattttgg ggatgccgct tacccccctg tggggcagca gccggcacgg
    11341 ttggcacggc gtttcggcgc agcagtccct gcttgccgaa gtgtttgccg ccatcggcgc
    11401 ggcggcaggt acggacaaac cggtccatgt gaaatatgcc gcgccggacg atgccaaaat
    11461 cctgctgggc aaggcaaccg tcctgcccga agacaacggc aacggcgtgg taatggtgat
    11521 tgacgacatc accgttttga tacacgcgca aaaagaagcc gcgtggggcg aagtggcaaa
    11581 acggctggca cacgaaatcc gcaatccgct cacgcccatc cagctttctg ccgaacggct
    11641 ggcgtggaaa ttgggcggga agctggacga gcaggacgcg caaatcctga cacgttcgac
    11701 cgacaccatc atcaaacaag tggcggcatt aaaagaaatg gtcgaggcat tccgcaatta
    11761 cgcgcgttcc ccttcgctca aattggaaaa tcaggatttg aacgccttaa tcggcgatgt
    11821 gttggcattg tacgaagctg gtccgtgccg gtttgcggcg gaacttgccg gcgaaccgct
    11881 gatgatggcg gcggatacga ccgccatgcg gcaggtgctg cacaatattt tcaaaaatgc
    11941 cgccgaagcg gcggaagaag ccgatgtgcc cgaagtcagg gtaaaatcgg aagcggggca
    12001 ggacggacgg attgtcctga cagtttgcga caacggcaag gggttcggca gggaaatgct
    12061 gcacaatgcc ttcgagccgt atgtaacgga caaaccggct ggaacgggat tgggactgcc
    12121 cgtggtgaaa aaaatcattg aagaacacgg cggccgcatc agcctgagca atcaggatgc
    12181 gggcggcgcg tgtgtcagaa tcatcttgcc aaaaacggta gaaacttatg cgtagcagcg
    12241 atattttaat tgtagacgac gaaatcggca tccgcgacct gctgtcggaa atcctgcagg
    12301 acgaaggtta ttcggtcgca ttggcggaaa acgccgaaga ggcgcgcaag ctgcgccatc
    12361 aggcgcgccc cgcgatggtg ctgctggata tttggatgcc tgattgcgac ggcatcaccc
    12421 ttttgaagga gtgggcgaaa aacgggcagc tcaatatgcc ggtggtgatg atgagcgggc
    12481 atgccagcat cgataccgcc gtagaagcga ccaaaatcgg cgcgctcgac tttttggaaa
    12541 aaccgatttc cctgcaaaag ctgctgtctg ccgtcgaaaa cgcgttgaag tacggtgcgg
    12601 cgcaaaccga aacggggcct gtattcgaca agctgggcaa cagtgcggcg attcaggaaa
    12661 tgaaccgtga ggtaggggct gcggtgaaat gtgcctctcc cgtacttttg acgggcgagg
    12721 cgggttcgcc gtttgaaacg gtggcacgct atttccataa aaacggtacg ccgtgggtca
    12781 gcccggcaag ggtcgaatat ctgatcgata tgccgatgga actgttgcag aaggcggagg
    12841 gcggcgtttt gtatgtcggc gacatcgccc agtacagccg caacatccaa gccggcattg
    12901 cctttattgt cggaaaggcg gaacaccgcc gcgtcagggt ggtcgcatcg ggcagcaggg
    12961 cggcaggttc agacggcatt gcctgcgagg aaaagctggc ggaactgctg tcggaatcgg
    13021 tcgtccgtat tccgcccctg cgtatgcagc atgaagacat tcccttcctg atacagggga
    13081 ttacctgcaa tgtggcggaa agccaaaaga ttgcgcctgc ctcattcagt gaagatgcgc
    13141 ttgccgcatt gacccgttat gaatggccgg gaaattttga ccagttgagc agtgtcgttg
    13201 caacgctgtt gttggaggcg gacgggcagg aaatcggtgc aggggcggtt tcttcccttt
    13261 tggggcagaa tgtgcctgcc gagggagcgg aagatatggt gggcgggttt aatttcaacc
    13321 tgcccctgcg cgaattgagg gaggaggtgg agcggcgtta tttcgagtac cacatcgccc
    13381 aagaaggtca gaatatgagc aaagtggcgc agaaagtcgg tttggaacgc acgcaccttt
    13441 accgcaaact caaacagctc ggcatcggcg tttcgcgccg ggcgggggaa aaaaccgaag
    13501 aataagcccg gacggccggt tttccggttg cgggcttttg ttttcagacg gcatttggtg
    13561 caaatgccgt ctgaaatcgt aaggggacgg attttatgac agaggacgaa cgtttcgcgt
    13621 ggctgcaatt ggcgtttacg ccctatatcg gcgcggaaag tttcctgctg ctgatgcgcc
    13681 gtttcggcag cgcgcaaaat gccctgtccg caccggcgga acaggtggcg gcactgatac
    13741 ggcacaaaca ggcgcttgag gcttggcgca atgcggaaaa acgcgctctg gcgcggcagg
    13801 cggcagaagc ggcgttggaa tgggaaatgc gggacggatg ccgcctgatg ctgcttcagg
    13861 atgaagattt ccccgaaatg ctgacgcagg ggctgaccgc gccgccggtt ttgtttttgc
    13921 gcggcaacgt gcggctgctg cacaagcctt ccgccgccat cgtcggcagc cgccatgcca
    13981 cgccgcaggc gatgcggatt gccaaagatt tcggcaagtc gttgggcggg caaaatatcc
    14041 ccgttgtgtc gggtatggct tccggtatcg atactgccgc ccatcagggc gcactggagg
    14101 cagaaggcgg caccatcgcc gtgtggggga cgggtataga ccgcatttac ccgccgtcca
    14161 ataaaaacct tgcctatgaa atcgccgaaa aaggattgat tgtcagcgag tttcccatcg
    14221 gtacgcgccc gtatgccggc aattttccgc gccgcaaccg cctgattgcc gccctgtcgc
    14281 aagtaacgct ggtggttgaa gccgccttgg aatccggttc gctgattacc gccggcctgg
    14341 cggcagaaat ggggcgcgaa gtgatggcgg tacccggttc gatagacaat ccgcacagca
    14401 aaggctgcca caaactgatt aaagacggcg caaaattggt ggaatgcctg gacgacatcc
    14461 tgaacgaatg cccggggcta ttgcaaaata cgggtgcttc atcatattct ataaataagg
    14521 atacgcccga cacgggacgc cgcgccgttc agacggcata cgccccgccg cctgccgcaa
    14581 aaatgccgtc tgaagcggcg gcaggcggca cagccgtcgg cggcatactg gataaaatgg
    14641 gtttcgaccc catccatccc gacgtgcttg ccggacagtt ggctatgcct gccgcagatt
    14701 tgtatgccgc actgttggaa ttggaattgg acggcagcgt tgccgcaatg cccggcggca
    14761 gataccagcg tatccgaact tgaacgcact ttatattaag gaacacgaat gaccgaagtc
    14821 atcgcctacc tcatcgaaca tttccaagat ttcgatacct gtccgccgcc cgaagacttg
    14881 ggtatgctgc ttgaagaagc gggtttcgat acgatggaaa tcggcaacac cctgatgatg
    14941 atggaagtat tgctcaacag ctccgaattt tccgccgaac ccgccgacag cggcgcattg
    15001 cgcgtgtaca gcaaagaaga aaccgacaac ctgccgcagg aagtgatggg gctgatgcag
    15061 tatctgattg aagaaaaagc cgtcagctgc gaacagcggg aaatcatcat ccacgcgctg
    15121 atgcacatcc ccggcgacga aattaccgta gataccgcca aagtgctgac cctgctgctt
    15181 ttatgggcaa acaagagcga gctgcccgtg ttggtcggcg acgagctgat gagcgcgctt
    15241 ttactcgaca acaaacccac gatgaactga agcggcttca gacggcccgc ccgaggccgt
    15301 ctgaaacgcc ggcatcaaaa ccaccatcca gagaacgaca aatggcgaaa aacctattaa
    15361 tcgtcgaatc cccgtccaaa gccaaaaccc tgaaaaaata tttgggcggc gattttgaaa
    15421 tccttgcatc ctacggacac gtccgcgacc tcgtccccaa aagcggcgcg gtcgatcccg
    15481 acaacggctt tgcgatgaaa taccaactca tcagccgcaa cggcaaacac gtcgatgcca
    15541 tcgtcgccgg tgccaaagaa gctgaaaaca tctacctcgc caccgacccg gatagggaag
    15601 gcgaagccat ttcctggcat cttttggaaa tcctcaaatc caaacgcggc ttgaaaaaca
    15661 tcaagccgca gcgtgtcgtg ttccacgaaa tcaccaaaaa cgccgtgctc gatgccgtcg
    15721 ccaatccgcg cgaaatcgaa atggacttgg tcgatgcgca acaagcccgt cgcgctttgg
    15781 actatttggt cggctttaac ctttcgccat tgttatggaa aaaaatccgc cgcggtttga
    15841 gcgcgggtcg tgtgcaaagt cccgctttgc gcctgatttg cgaacgcgaa aacgaaatcc
    15901 gcgcgtttga agcgcgggaa tattggacgg tgcatctcga cagccacaaa ggccgcagca
    15961 agttcaccgc caaactcgcc caatacaacg gcgcgaaact cgaacaattc gacctgccga
    16021 acgaagccgc gcaagccgac gtgttgaaag aactcgaagg caaagaggcc gtcgttaccg
    16081 ccattgaaaa gaaaaagcgc agccgcaatc ccgccgcgcc gttcaccact tctaccatgc
    16141 agcaggatgc tgtgcgcaaa ctcggcttca ccaccgaccg caccatgcgt accgcccaac
    16201 agctttatga aggtatagac gtagggcagg gcgcgatcgg tctgattacc tatatgcgta
    16261 ccgacagcgt gaatttggcg gatgaagcgt taaccgaaat ccgccattac atcgaaaaca
    16321 aaatcggcaa agaatatctg ccgagtgccg ccaaacaata caaaaccaaa tccaaaaacg
    16381 cccaagaagc gcacgaagcg atccgcccga cttccgtgta ccgcacgccc gaaagcgtca
    16441 aacccttcct gagcgcagac cagttcaaac tctatcaaat gatttggcag cgcaccgtcg
    16501 cctgtcagat gacgcctgcc aaattcgacc aaaccaccgt cgatattacc gtcggcaaag
    16561 gcgtattccg cgtaaccgga caagtgcaaa ccttcgcagg cttccttagc gtttacgaag
    16621 aaagcagcga cgacgaagag agtgaagaca gcaaaaaact gcccgaaatg agcgaaggcg
    16681 acaaattgcc cgtggataaa ctttacggcg aacaacactt taccactccg ccgccgcgct
    16741 acaacgaagc gacgctggtt aaagcccttg aagaatacgg catcggccgc ccttcgacct
    16801 acgccagcat catctccacg ctcaaagacc gcgaatacgt tacccttgag caaaaacgct
    16861 tcatgcccac cgacacaggc gacatcgtca acaaattcct gaccgaacac ttcgcccaat
    16921 acgtcgatta ccacttcacc gccaaactcg aagaccagct tgacgaaatt gccgacggca
    16981 aacgccaatg gattcccgtg atggacaaat tctggaagcc gtttatcaag caagtggaag
    17041 aaaaagaggg catcgaacgc gccaaattta ccacgcagga actcgacgaa acctgcccca
    17101 aatgcggcgg acacaaactg caaatcaagt tcggcaaaat gggtcgcttc gtcgcctgcg
    17161 ccggctatcc cgagtgcagc tacacgcgca atgtcaacga aaccgccgaa gaagctgccg
    17221 aacgcatcgc caaagccgaa gccgaacagg cagaactcga cggacgcgag tgcccgaaat
    17281 gtggcggtcg cctagtgtac aaatacagcc gcaccggcag caaattcatc ggctgcgcca
    17341 actatccgaa atgcaaacac gtcgagccgc tggaaaaacc gaaagatacc ggcgtccagt
    17401 gtccgcaatg caaaaaaggc aacctcgtcg agcgcaaatc ccgctacggc aaactgtttt
    17461 acagttgcag cacctatccc gactgcaact acgccacttg gaacccgccc gttgccgaag
    17521 aatgcccgaa ctgccattgg ccggtcttga ccatcaaaac caccaaacgc tggggcgtgg
    17581 aaaaagtttg tccgcaaaaa gaatgcggct ggaaagaaca gattgaaccg cctgcaccga
    17641 aagagtgaga ttaggttggt ttgaaagaga aaaggtcgtc tgaaaaattt tcagacgacc
    17701 tttgcttttc tgtgattggt ttattggaat ccgcgtgttg ttttaaagtc cgataaaatc
    17761 cggttcattt caggcgcaaa caaggcgatg taatcgtaag atagaccgcg actggcactg
    17821 ggatggggaa agcagacgac ttcgcaatct tcaaacgatt ggaatttgac attgaaacgt
    17881 gtaccgtcaa attctttttg caccgtctcc agcggtttgg tctgcttacc gaccaactgc
    17941 tcgaagcgtg gcagtacatt ttggttgttc agaaaatccg ccaacctgct gcccatgaag
    18001 aggatgactt tcggacgcag tttttcgatg tggtagagaa aattatcgat gtgctcgggt
    18061 tgtgtgaact tgtcgggatt gtcgatagtg ttgccctgtg tagcagccca gttggtttga
    18121 accagggatt tttcaaatgc accgcccaat ccattttcgt ctaaggggtg tccccacatt
    18181 tcaaaccaat tttttatcgt attgtcgtaa cgccactttt ttgcctgctc tccgaaatag
    18241 agggatttgt ttgcaaatgt atggtcgatt ttgttttcag ggagtttgta ttcacctgct
    18301 acataagcag cctcatcggc tttactccaa ccccattcat agccacaaat cattaagcca
    18361 tgtttgtcgt tgtagccttt gaacaggctg ttgctcaaat tcaaatcctt catcatgaac
    18421 tcttcctttt aaaatttaag agcgattgac ttcaatgttt ttagatgggg tggaaaaatc
    18481 cttgtgtagg caacataaat tcaataaatt tcttgataat tcgaaaccta ctaatagcgc
    18541 acctataaaa gctttttcat tacgttcagc atgacggtca cgtcgttcat attttttacg
    18601 cttgctgttc cctgttatta cagctaagcc aagtgatatg gcgagaattg cccaaacaat
    18661 agtacttaat aacaattttc cccatactat caatagggaa agaaaaaacc ttttagtatt
    18721 aggtcgatag attataatcc atgcccatga aaatgcaata agagttatac ataagataca
    18781 agctgatagg atttctttat ttttaattaa ataacttagg gcgatgagga tgacaggtgt
    18841 caagaaaaca atagttacat cccgatagct ataaaagaaa actgccccat tttgaatgtg
    18901 gaaatgtgca cagaatccaa tatagctaag gataatagtt aatataataa aaaaagacca
    18961 ccaagggtga agagatagga attccatgtt ttcccttttt ttgtaaaaag gaaaaaatct
    19021 tatctaatag ttaattgcct aaccagccag aagaagttta aaatctatcc caataattca
    19081 accatctata cagaaagttc agcttatgga aacccacgaa aaaatccgcc tgatgcgcga
    19141 attgaataaa tggtcccagg aggatatggc ggaaaagctg gcgatgtcgg caggcgggta
    19201 tgccaaaatc gaacgaggcg aaacgcagtt gaatatcccg cgtttggagc agttggcgca
    19261 gattttcaaa attgatatgt gggacttgct caaatcgggc ggcggcggga tggtgttgca
    19321 gattaacgat gtggatacca acagcgggga atttgcaatc tataccgctc aggatgcatc
    19381 ggtaaagctg gatttgttaa aaatggaatt aaaacactgt aaagaaatgt tggaacacaa
    19441 agacaaagaa atcgagctgc tccgcaagct gaccgaaacc gtttaaacag atatgccgtc
    19501 tgaaaaaagt tttcagacgg catattcttt gacaggtctt gtataatacc gtttgaactt
    19561 acaggttttt gattatggcg gcaggcaaac ataccaaaca cagtaaccgg gtacgcatta
    19621 tcggcgggca atgccggggc aggaaattaa gtttcacatc cgccgacgga ctgcgtccga
    19681 cacccgacag cgtgcgtgaa aagctgttta actggctggg acaggatttg acgggtaaaa
    19741 cggttttgga tctcttcgga ggcagcggcg cactcggtat agaagccgct tcgcgcaacg
    19801 ccaaacgcgt gctgatttcg gataacaacc gccaaaccgc gcagaccttg cagaaaaaca
    19861 gtcgcgaact gggtttgggg caggtgcaaa tcgtctgttc agacggcatc gcatatttga
    19921 agaccgtatc cgaacagttt gatgttgtct ttctcgaccc gccgtttgca tggcaggact
    19981 ggcaaatcct gtttgatgcc ttgaagccgt gcctgaaccc ccggtcattc gtctatctcg
    20041 aggcgggtac gctgccggat attcccgatt ggctgacgga atatagagag gggaaatcgg
    20101 ggcagagtac atttgaatta agggttttcc aagtggctga ataatatgcg ctttgataat
    20161 catttccgag ttgtaaacat tcgtttgcaa ccgtccggtt caaaaaaacc ttgtgctata
    20221 atccgcgccc gcccggtttt gataatttag tggaaaagga aaagaaatgt cgctttttat
    20281 taccgacgag tgcatcaact gcgacgtatg cgaacccgaa tgccccaatg atgccatttc
    20341 ccaaggcgag gaaatttacg aaatcaaccc caacctctgc acgcagtgcg tcggacacta
    20401 cgatgagccg cagtgccagc aggtttgccc ggtggactgc atcctgattg acgaagaata
    20461 tcccgaaacc catgacgagt tgatggcgaa atacgaaaag attatccagt ttaaataaat
    20521 tctttttaaa acatcaaatt atgtctgttt tgaaataaaa tcaaaaaaaa aacttgacgg
    20581 aaaagcaagc cgctaataaa ctaacgttct cttttggagg ggttcccgag cggtcaaagg
    20641 gggcagactg taaatctgtt gcgaaagctt cgaaggttcg aatccttctc cctccaccaa
    20701 aattcttact tggggcagta gcgagtaatg cgggtgtagc tcaatggtag agcagaagcc
    20761 ttccaagctt acggtgaggg ttcgattccc ttcacccgct ccaaacaatt aggcccatgt
    20821 agctcagggg tagagcactc ccttggtaag ggagaggccg gcagttcaaa tctgcccatg
    20881 ggcaccatct ctcgattatt catttcttta aggcttagat atataggata ttg
//
LOCUS       DAWKQB010000019        20768 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.019, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000019 DAWKQB010000000
VERSION     DAWKQB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.019"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJL14_000548"
     CDS             complement(97..1116)
                     /gene="lepB"
                     /locus_tag="WJL14_000548"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ7655662.1"
                     /translation="MNIMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJL14_000549"
     CDS             complement(1259..3052)
                     /gene="lepA"
                     /locus_tag="WJL14_000549"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ7655663.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPAGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     IANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(3197..3898)
                     /locus_tag="WJL14_000550"
     CDS             complement(3197..3898)
                     /locus_tag="WJL14_000550"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ7655664.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            4084..5196
                     /locus_tag="WJL14_000551"
     CDS             4084..5196
                     /locus_tag="WJL14_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7655665.1"
                     /translation="MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            5230..6207
                     /locus_tag="WJL14_000552"
     CDS             5230..6207
                     /locus_tag="WJL14_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ7655666.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCWKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            6211..6561
                     /locus_tag="WJL14_000553"
     CDS             6211..6561
                     /locus_tag="WJL14_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ7655667.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            6566..7345
                     /locus_tag="WJL14_000554"
     CDS             6566..7345
                     /locus_tag="WJL14_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ7655668.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            7371..7586
                     /locus_tag="WJL14_000555"
     CDS             7371..7586
                     /locus_tag="WJL14_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ7655669.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            7687..7998
                     /gene="grxD"
                     /locus_tag="WJL14_000556"
     CDS             7687..7998
                     /gene="grxD"
                     /locus_tag="WJL14_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ7655670.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            8104..8730
                     /gene="upp"
                     /locus_tag="WJL14_000557"
     CDS             8104..8730
                     /gene="upp"
                     /locus_tag="WJL14_000557"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ7655671.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            8753..9073
                     /locus_tag="WJL14_000558"
     CDS             8753..9073
                     /locus_tag="WJL14_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ7655672.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            9324..9746
                     /locus_tag="WJL14_000559"
     CDS             9324..9746
                     /locus_tag="WJL14_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655673.1"
                     /translation="MKKMFLSAVLLLSAAAQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRRATMKFQNGEFMYTVWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPQTAQANFNDADFSR"
     gene            9764..10525
                     /locus_tag="WJL14_000560"
     CDS             9764..10525
                     /locus_tag="WJL14_000560"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ7655674.1"
                     /translation="METVKPIMLIVRPSGRAKDDVETCRRAGWQAEVLSPIEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRVLERCLGRTV
                     IAPEDGNDSEAVLRLTVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            10540..11874
                     /locus_tag="WJL14_000561"
     CDS             10540..11874
                     /locus_tag="WJL14_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ7655675.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            11871..13088
                     /locus_tag="WJL14_000562"
     CDS             11871..13088
                     /locus_tag="WJL14_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ7655676.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVLNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            13163..14227
                     /locus_tag="WJL14_000563"
     CDS             13163..14227
                     /locus_tag="WJL14_000563"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ7655677.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEDDIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGSS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            14413..15792
                     /gene="radA"
                     /locus_tag="WJL14_000564"
     CDS             14413..15792
                     /gene="radA"
                     /locus_tag="WJL14_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ7655678.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NSRFQSWAADTSTVQSLSAVTAAEVPRNPTGMSELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNVKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(16068..16547)
                     /locus_tag="WJL14_000565"
     CDS             complement(16068..16547)
                     /locus_tag="WJL14_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ7655679.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTIGE"
     gene            complement(16679..17038)
                     /locus_tag="WJL14_000566"
     CDS             complement(16679..17038)
                     /locus_tag="WJL14_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ7655680.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(17077..20691)
                     /gene="recB"
                     /locus_tag="WJL14_000567"
     CDS             complement(17077..20691)
                     /gene="recB"
                     /locus_tag="WJL14_000567"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ7655681.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRGRLDDVLQVLESKGIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPELLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVEMTEEDGDRLLIPAQDFWRERVSNDPVLAALALKRKAVPQTVLAQISRYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTICRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHADT
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYNAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYAQEKDGITMLKQNWRRVADNAPAGTDFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVAAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEMWLPAVAEM
                     AEACQKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVASARYFKLRGQPPAAVSVRYLFLRGIDGKGGGVWHWDIDAAALEQIK
                     "
BASE COUNT         4742 a         5426 c         5841 g         4759 t
ORIGIN      
        1 gtaggaaaat ccgacaacgt tagatccgcc tgcaaaagtg aggaatagca aatgccgtct
       61 gaaactattt tcagacggca ttcttggctt cctggcctaa cggattgccg taccggccct
      121 gccgaaatcg ccgaagttca tcaaaatgaa cattgccttg ccgacaacca gcttgtcatc
      181 cacaaatccc cagtagcgcg aatcggcact gttgtcgcgg ttgtcgccca tagcgaaata
      241 gcgtccttcg ggaactttgc acacgaaacc gctgccgtcg tcggcatatt ggcagtgttc
      301 caaaccgctt tgctctatgg aatatccgtt ttcagacata atatcggagg tatatttgcc
      361 caatacgggc agggaaacgg caggctgtcc ttcttttttc agaatattga aggatttgcc
      421 gtccaaaccg ctgtggaaca tatccgtatt gtggatttcg gatgggtcgg tgtcgtcggg
      481 ataacggtat gtgccgtcag gaatatcgga agcaggtttt ccgtttaccg tcaaaacttt
      541 attccgatat tcgaccacat cgcccggaat gccgacaata cgcttgatgt aggtcatctc
      601 cggttgcaaa ggataattaa aaacgaccac atcgccccgt tcgattttgc ccgtggggat
      661 gaatacattg tttaaaacgg gtacgcgcag gccgtaggaa aatttgccga ccaaaatgaa
      721 atcgccctta atcaggcccg ggcgcatcga gctggacggg atttggaacg gttcggcaat
      781 aaacgaccgg atgaggaaca ataccaaaac ggtagggaag aaactgccga aataatcgcc
      841 gaagtggctg ctttccgaga tttcgggatg agtcttcagg cggtatttgt atacccccca
      901 agccgtaccg cacaatacaa cgaaaatcag gaaaacggcg gtaaagctca taaacaggga
      961 caaagcggca aacacgccga ccgccgtcag gatataggcg tattccagac cggaactcca
     1021 ttcaccgttt tcctgccgct ttttgtcgct tttgaaataa aggatgatgc cggcaagcag
     1081 cgcggcagcc gcgcccgaca ttagcataat gttcattgtt gttccttaac ggttaaaaac
     1141 ccgcccgtcc gtgcaaccgt ttttaagagg cggtaaatca caaagtttgt tggcggacgt
     1201 gctctcttac aatcagggcg gtttaagggg catgatgcac tgccccgtgt gccggatatt
     1261 atttgtcgct cacctgcaaa attgccaaga acgcgctttg cgggatttcc acattgccca
     1321 cttgtttcat acggcgtttg cctgcttttt gtttttcaag cagttttttc ttacgcgtaa
     1381 tatcgccgcc gtaacatttt gccaaaacgt ttttacgcag ggctttgacg ttctcacggg
     1441 cgataatctg actgccgatg gcggcttgga cggcaatatc aaacatttgg cgcggaatca
     1501 gctcgcgcat tttcgatgcc agttcgcggc cccggtgaac cgcgctttga cggtggacaa
     1561 tcaggctcag cgcatcgact ttttcaccgt tgaccatgat atccagctta atcaaatcag
     1621 acggttggaa ctctttgaaa tggtagtcca acgaagcata gccgcgcgaa gtagatttga
     1681 gtttgtcgaa aaagtccatc accacttcgt tcatcggcaa gtcgtaagtc agcatcactt
     1741 ggcggcccat atattgcata ttgacttgca cgccgcgctt ttggttgcac aaagtcatga
     1801 cgttgccgac atattcctgc ggcacgagga tggttgcagt gataatcggc tcgagtatgg
     1861 tttcgatgct gccgatgtcg ggcagtttgg acggattttc gacttcgatt ttctcgccgt
     1921 ttttcaacac gacttcataa accaccgtcg gcgcggtggt aatcaagtcc atgtcgaact
     1981 cgcgttccaa acgttcctgc acgatttcca aatgcaacag gcccaagaag ccgcaacgga
     2041 agccgaaacc caatgcttgg gaaacttcag gctcgaattt caacgaagca tcgttaagct
     2101 gcaatttttc caaagcgtcg cgcaaggctt cgtagtcgtg gctttccacc ggataaagtc
     2161 cggcgaatac ctggctttgc acttcttgga agccgggcag cggctcggag gcggggttgg
     2221 caatcaaagt aactgtatcg ccgacttttg cctgtcccaa ttcttttacg ccggtaatca
     2281 aaaagcccac ttcgccggct ttgagttctt gtttttgaac tgatttcggc gtgaatacgc
     2341 ccagctgctc aacctgcgtt tccgccttgg tgctcataaa gcgcactttg tctttcagct
     2401 tgatggtgcc gtttttcaca cgaatcagca taaccacgcc gacatagttg tcaaaccacg
     2461 aatcgacaat caccgcttgc agcggcgcat tttcatcgcc ggccggtgcg gggattttgg
     2521 caacgatttc ttccaaaacg tcttccacgc cgatgccgct tttggcggaa cattgcaccg
     2581 cgccgacggc atcgatgccg atgatatctt cgatttcctg ttccacgcgt tcggggtcgg
     2641 cggcgggcag gtcgattttg ttcaaaacgg gcacgacttc aacgcccaaa tcaatcgcgg
     2701 tgtagcagtt cgccacggtt tgcgcttcca cgccttgaga tgcgtcaacc accaaaagcg
     2761 cgccttcgca ggccgacagc gaacgggaaa cttcgtaaga gaagtcgacg tgtcccggcg
     2821 tgtcaatcag gttgagctga tacacctgcc cgtcgcgcgc tttgtagttg agcgcggcgg
     2881 tttgcgcttt gatggtgatg ccgcgctctt tttcgatgtc catagaatcg agtacctgcg
     2941 tactcatttc gcgcaaatcc aagccgccgc agtattggat aaaacggtcg gcaagcgtcg
     3001 atttgccgtg gtcgatgtgg gcaatgatgg agaaatttcg gatatttttc attagagtta
     3061 ttttgaatgt cggacagtgg gttttggaaa tgccgtctga acaaacggcg ctgcgtccga
     3121 atgtcgggcg caacgggata aacagcccga cattctagcg gaaaaccgct gttttggcat
     3181 aagtttgata aaggtcttat aaagatttga cgatttctgc caccattttt gcggaatttg
     3241 ccgccgccgt tttcaagaac tcgtcaaagc tgatgtctgc cttttcgtct gccgaatcgg
     3301 aaaccgcgcg gatgatgacg aaaggcgttt ccaactgatg acaggtttgg gcgattgccg
     3361 ccgcttccat ttctaccgcc ttaacttcgg ggaagtgctt gcggatttcc gccacgcctt
     3421 cgctgctgtg gacgaagcgg tcgccgctga caatcaggcc ttgttctacc gccgcgcctt
     3481 caaacacttc cgccgcctgt tttgctttgc cgaccaagag gccgtctgaa acaaatacgg
     3541 cgggcagttg cggcacttgt ccccaaacat agccgaatgc ggttacatcg acatcgtggt
     3601 gcgcgatttc tgtgccgacc acgacatcgc cgactttcaa acccttgccc aagccgcccg
     3661 cgctgccggt gttgataacg cagtccggtg cgaattcacg gataatccaa gccgttgcaa
     3721 ccgccgcgtt gaccttgccg atgccgctca atgcaagcac catgcgtttt cccgctaatt
     3781 cgccttcata ggcggcaaac ttgccgaaag agacggcttt gacattttcc atcatctcgc
     3841 gcaaaagctc gatttcttgt tccattgcgc cgataacggc tactgttttc aaagacatat
     3901 tgctgacctg ttgtgaattt cggatagaat gcctgattat acacgctaac acggcaggat
     3961 tgagtggagg tggtttgtcc gtgccgtctg aaacggtttc agacggcacg gcgggttttt
     4021 ggtagaatgg gaaggtacag attgtttgaa gattagggga cgaggatgtt taccgatgaa
     4081 aatatgaccg caaaggaaga actgttcgca tggctgcgcc atatgaacaa aaacaaaggt
     4141 tccgacctgt tcgtgacgac ccatttcccg cccgcaatga agctggacgg caaaatcacc
     4201 cgcatcacgg acgaaccgct gacggcggaa aaatgtatgg aaatcgcctt ttcgattatg
     4261 agtgcgaagc aggcggaaga attttcatcg accaacgagt gcaacttcgc catcagtctg
     4321 ccggacacca gccgcttccg cgtcaatgcg atgatacagc gcggtgcgac ggcgttggta
     4381 ttccgtgcga ttaccagcaa gattcccaag tttgaaagcc tgaacctgcc gccggtcttg
     4441 aaggatgtcg cgctgaaaaa acgcgggctg gttatttttg tcggcggcac cggctcgggc
     4501 aaatcgactt cgcttgcctc gcttatcgac taccgcaatg aaaattcgtt cggacacatc
     4561 atcaccatcg aagacccgat cgagtttgtc cacgaacaca aaaactgcat catcacccag
     4621 cgcgaggtcg gcgtggatac ggaaaactgg atggcggcgt tgaaaaacac gctgcgtcag
     4681 gcgccggatg tcatccttat cggcgaaatc cgcgaccgcg aaacaatgga ctacgccatt
     4741 gcctttgccg aaacggggca tttgtgtatg gcgacgctgc acgccaacag caccaatcag
     4801 gcactcgacc gcatcatcaa ctttttcccc gaggagcggc gcgaacaatt gctgacggat
     4861 ttgtcgctca accttcaggc gtttatttcg caacgcctcg ttccgcgaga cggcggcaag
     4921 ggcagggtgg cggcagtcga ggtgctgctc aattcgcccc tgatttcgga gttgattcac
     4981 aacggcaaca tccatgaaat caaagaagtg atgaaaaaat ccactaccct gggtatgcag
     5041 accttcgatc aacaccttta ccaattgtat gaaaaaggcg atatttccct gcaagaagca
     5101 ttgaaaaatg ccgattccgc acacgatttg cgtttggcgg tacagttgcg cagccgccgc
     5161 gcgcaaagtt ccagccccga tttggaactg ctctgatggc ggtatggatt tccggacgga
     5221 tggtttgaaa tgatttatcc gtggcataat gagcaatggc ggcagattgc ggaacattgg
     5281 gagcgtcgtc ccaatgcatg gctgtttgcc ggcaaaaaag atacggggaa aactacattt
     5341 gcccgctttg cggcgaaggc actgttgtgc gaaacccctg caccgggctg caaaccctgt
     5401 ggcgaatgta tgtcctgcca tctgtttgga cagggaagcc atcccgattt ttacgaaatc
     5461 acccccttgt cggacgaacc cgaaaacgga cgcaaactgt tgcagatcaa aatcgatgcc
     5521 gtcagggaaa tcatcgataa tgtgtacctg acttcggtac ggggcggttt gcgcgtgatt
     5581 ctgattcatc ctgcggaaag tatgaatgtc caagccgcca acagtttgtt gaaagtgttg
     5641 gaagaaccgc cgccacaagt ggtctttttg ctggtcagcc acgcggcgga caaggtttta
     5701 ccgaccatta aaagccgctg ctggaagatg gttttgcccg cgccttccca tgaagaggca
     5761 ttggcatatc tgcgtgaaag gggtgtggcg gaacctgagg aacgtctggc tttccattcc
     5821 ggagcgccgc tgtttgatga ggcggacggt gtccgtgcgt tgcggattaa actgttggat
     5881 attttggcag aaccgaggtt gttgaagatt ttggattacg ccgcgctttt cgataaggaa
     5941 aaacttccgc tcgccgtatt tgtcgggtgg atgcagaaat ggctggtcga tttgggattg
     6001 tgcctgcaac acatgaaacc cgtctattat cccgcttatg aagacaggct gcttcagacg
     6061 gcatccggtt tccgcctgcg caatgtattt gcggcggagg atatgctcaa acagcttgcc
     6121 ccctacgggt ttcatacttt aaatgtcaaa atgcagatcg agcatctgct catcaactat
     6181 ttggaattga agaaagagaa cgggtgaatt atgtcagacg gacaaaatat tccggcaaaa
     6241 atgatgtcgt tgcagctgaa agacatgaat ctgctgtaca gctcctacat gccgtttttg
     6301 gaacacggcg gtctgtttgt gcagaccaac gacgtatttt ccatcgggga cgatattctg
     6361 cttgccgtag aaatcctcaa cttccccaaa ctgttcctgc cgaccaaagt cgcctggatc
     6421 aatcctgcgc gtacttcctc caaacccaaa ggggtggggc tggcattcac aaaacacgaa
     6481 aactgcctga aagtcaaaga ccagatcgaa gtcgaactgg gcaacacaat cggcggcagc
     6541 agacctacgt ttaccatgta acgccatgca tatcatcgat tcgcactgcc acctcaattt
     6601 tgaaggtttg aaagaacgcc tgcccgaagt tttgtccaac atggaagcaa acggcgtggg
     6661 gcaggcgctc gccatcagcg tcagtaggga aagcttctcc gaagtctttg ccatcgccga
     6721 agcgcacgaa cacatctatt gcaccatagg cgtacatccc gacagcaagg aagccgaaga
     6781 attttctatt gcggaaatgg ttgccgcagc cgcctcgccg aaagtggtcg gcattggcga
     6841 gacgggtttg gattattact ggtgcaaagg cgatttgtcc tggcaacaca aacgctttgc
     6901 agaccacatc gaagcagcca atcaaaccgg actgcccgtt atcgtccata cgcgtgatgc
     6961 ggcggcggac accttgtcta tcctgaaaga atgccgggtt aattcgggcg ttatccactg
     7021 tttttccgaa gacatcggtt ttgcccgtgc agcaatggat ttggggcttt atatttcttt
     7081 ctcgggaatc gttaccttta aaaacgcacc cttggttcag gaggcggcaa aatacgttcc
     7141 ggacgaccgc attttggtgg aaaccgatgc gccgttcctc gcccccgttc ccaaacgcgg
     7201 caggcagaac gaaccggctt ttgtgcgcca taccgccgaa catatcgcca aattgcggaa
     7261 ccaaacattg gaacaggttg cggcatatac gacggaaaac ttttaccggc tgtttaaaaa
     7321 agtacccgat atgcggaccg tctgaccctg taccgacgat aaggaaaacc atgaaggcaa
     7381 ttcatccgta tgcatgtccg cgctgctgcc ggctgcctgc caacacgttt cggacaggca
     7441 tggcaaattc cgcttccaaa ttctgcattg ccaaaggcgg cagacgggaa gtaaaaaaag
     7501 acgaaagcgg cggcggatat gccctgtgcc atttgccgga cagcaggatt gtcgaggagt
     7561 gggaatattt ccgttcacaa cattgatact gcgcgatata cggcaaatat tgtgggaagt
     7621 ttccgctttt gcgtataatg cgtcctacct gacaaatttt gtcaacttta tcaaaaggaa
     7681 taagcgatgg cttccatcca cgaccaaatt aaagaagtag taacgacaca ccgcgtcgta
     7741 ttgtttatga aaggtacgaa gcagtttccg caatgcggtt tctcttcccg cgccgtgcaa
     7801 atcctgaacg cggcaggctg caccgattac gttaccgtca acgtattgga aaatcccgaa
     7861 gtacgccaag gcattaagga atacagcgac tggccgacca tcccccaact ttatgtgaac
     7921 ggcgagtttg tcggcggttc ggacatcctg atggaaatgt atgaggcagg cgagctgcaa
     7981 gagctgctga aagcctgatg gattcggcaa tgccgtctga acgtgtttca gacggcattt
     8041 tcttttccgg caaatcaaaa aaaagtataa tggcgcgtct caaaatcaca ctggaacacc
     8101 gcgatgaacg ttaatgttat caaccatccg ctcgtccgcc acaaattaac cctgatgagg
     8161 gaggcggatt gcagcaccta caaattccgg acgcttgcca tcgagctggc gcgcctgatg
     8221 gcatacgagg caagccgtga ttttgaaatc gaaaaatacc ttatcgacgg atggtgcggt
     8281 cagattgaag gcgaccgcat caagggcaaa acattgaccg tcgttcccat tctgcgtgca
     8341 ggtttgggta tgcttgacgg cgtactcgac ctgattccga ctgccaaaat cagcgtagtc
     8401 ggactgcagc gcgacgagga aacgctgaaa cccgtttcct acttcgagaa attcgtggac
     8461 agcatggacg aacgtcctgc cctgattatc gacccgatgc tggcgacggg cggttcgatg
     8521 gttgccacca tcgacctttt gaaagccaag ggctgcaaaa acatcaaggc actggtgctg
     8581 gttgccgcgc ccgagggtgt gaaggcggtc aacgacgcgc atcccgacgt tacgatttac
     8641 accgccgcgc tcgacagcca cttgaacgag aacggctaca tcatccccgg cttgggcgat
     8701 gcgggcgaca agattttcgg cacgcgctaa ctgactgatt ttcggagttg atatgaattt
     8761 tcaagactat ctcgccacat ttccttcaat cgaccatctg ggcggtttgg acgttcagga
     8821 tgcagatggc aaaacggttc accacattcc tgccgttcag ggtaagctcg gttcgctcaa
     8881 gttgtacaac gctttggcag aacgttttga cggaaaattg gacaaagaag cggcagaaca
     8941 gggtttgata tggtttgccg aacacgttgc cgacgcgcgc gcccatcccg gcaagcatcc
     9001 caatatcgac ctgcttgaaa aagttgtgca aagcggcgaa accttgctgc tcaagccgct
     9061 tgccgcgcaa taattttcga ccatgccgtc tgaaatccgt ttcagacggc attttgtcgg
     9121 aaagaagacc gtaaaacggg cattttcttt tctatttcag gatacgggca atgatgtttc
     9181 aacacacagg acgacacata aagcgttgcc ctatgtgttg ccctaatttg gaaggggtta
     9241 cacccctctc aaataacatc tgatgctgct gccacgaagg acggatgtcc aaatggcgga
     9301 gtttcaacca ttaaggaaat acgatgaaaa aaatgttcct ttctgctgta ttgcttctgt
     9361 cggctgccgc ccaaacggtg tgggcggata cggtgttttc ctgtaaaacg gacaacaaca
     9421 aatatataga agttcaaaaa atcaaccgca atctttacga atattcgttc ggcagtgcgg
     9481 caaaaaaaga aattgccata cgcaacagca aagctgacct gttggggcgt tccgacaggt
     9541 ggcaaggcat gggtagcgga cgccgggcaa cgatgaaatt ccaaaacggc gaatttatgt
     9601 acaccgtatg gacgggcttc gattccgtga ctcatacgga aagcagcggt gttgttgtgg
     9661 agcgtagggg caaggaagtc gcacgggttg gctgtacgcc gcaaaccgcg caggcgaatt
     9721 tcaacgatgc tgatttttcc cggtaatcgg ggcggataag gcgatggaaa cagtgaaacc
     9781 catcatgctg attgtccgac cgtcgggcag ggcgaaggac gatgtggaaa cctgccgtcg
     9841 tgccggatgg caggcggaag tgttgagccc gattgaaatc gaagcagatg ctgccggact
     9901 ggaacttttg tccgaacaat atgcccgtgc ggatgccgtg ttttgggtca gtccgaccgc
     9961 cgttgaaacc gccgtcccgt accttaacct ttcagacggc ataaagatgc acatttccgt
    10021 agggcagggc agccgccgcg tattggaacg ctgtttgggc agaacggtca tcgcgcctga
    10081 agacggcaac gacagcgagg cggttttgcg cctgacggtt tggaacagtc tgcccgaagg
    10141 tgcgcgcgta ttgtttgtgc gcggacacgg cgggcgggat tttctgatga atgccttgca
    10201 ggagaaaggt tttcggacgg aggtggcaga agtctatttc agacggcata aacctttgaa
    10261 ctttcaaaat ttccaaaccg aaaatattgc cgccgcctat attacgtcca cagaactcgt
    10321 gcgcttgctg ttcggacagc ttccgccgca attttcccga ttcttcaaat ccttgctata
    10381 ctttacccat catccgcgca ttgcggaggc attgaagcgc gaaggcgtgt gttcggtcga
    10441 aaccgtccct acgctggaag ccgcgctttc ccattcttcc atttccgttt cagacggcat
    10501 ggtctttccc ggaacctcaa attaataagg agcaaaacgg tgggcgaacc tgaaaacaaa
    10561 tcatccgaac ccgtacgcga gatacaggca tcaaaagaaa tgccgtctga aacctcttcc
    10621 ccacgcaaag aaaacgaaac agaagtacac attcctgccg ctccttttat cgtcaaacag
    10681 tccggcagca acgctttggc agtctgcgcc ctggtattgg cggcattggg tttgggtaca
    10741 agtggttttt tgtttgtcca aggacagaat gtcttgaaaa accaagagct ggcattcaac
    10801 caaaaaatcg acaaagccgc cttgggcgag tcggaaaacg ccgccctgtt gaaagacaac
    10861 ctcaaccggc aagccgccat acaatcagag ctcgaccgtt tggacggaaa cgtcaaagca
    10921 aacggcgaac aaatcttgga aatgcaaaaa tcctatcgcg agttgaccaa aggacgcgcc
    10981 gattggctgg tggacgaaac cgagaccata ctcaatctgg cggcgcaaca gctggtgttg
    11041 actggcaata tccaaacggc agtcggcgta ttggagcata tcgacagccg cctgtcccgt
    11101 ttcaatcagg cagagcttct gccgatcaag caggcggtca gcagcgactt ggcggaactg
    11161 aaaaaccgtc cctatgtcga tatttccggc acggcattgc gcctcgacag gctggaaacc
    11221 gccgtatccg gactgccgct gatgctcgac ggcgtattga aaccgggcgt acaggtgaag
    11281 aacgaagccg cttctgcttc atggtggcag aacgtatggg aaaaatccct cggcacattg
    11341 aaggggctgg tcgaaatccg ccgtttggaa aacaacgatg ccatgctgat ttctcccgaa
    11401 caggcatatt ttgtgcgtga aaacctgcgc ctccgccttt tggatgcgcg gactgcatta
    11461 atgcagcgca acagcgaagt ctatcagggc gatttgaaca atgccgaagc cgccgtcaga
    11521 cagtatttcg atgccaagtc tcccgccacg cagtcgtggc tgaaagaact ggcggaattg
    11581 aaggcgttgg atgtgcgcat gacggcggat gacggtttga aaaacagcct aaatgccgtc
    11641 cgcgcctatc gcgacggtac gcgcatgacg gcggcggaaa atcaagaagc agaacaggcg
    11701 gcttccgaac cggcaaacga aaacacagct tccgaaccgg ctgccgcatc ggatgtgaag
    11761 accatagaag caccgtccct gccttcggaa cgcaaaccgg aacagcctgc aaaaaaacag
    11821 cccgtaccgg aaaaggcagg gcgttcgccg tccgctaaag gagaacgcgc atgaaaacgg
    11881 tagtctggat tgtcgtcctg tttgccgccg ccgtcgggct ggcattggcg tcgggcattt
    11941 acaccggcga cgtgtatatc gtactcggac agaccatgct cagaatcaac ctgcacgcct
    12001 ttgtgttagg ttcgctgatt gccgtcgtgg tgtggtattt cctgttcaaa ttcatcatcg
    12061 gcgtactcaa tatccccgaa aagatgcagc gtttcggttc ggcgcgtaaa ggccgcaagg
    12121 ccgcgcttgc tttgaacaag gcgggtttgg cgtattttga agggcgtttt gaaaaggcgg
    12181 aacttgaagc ctcgcgcgta ttgggaaaca aagaggcggg ggataaccgg actttggcat
    12241 tgatgttggg cgcacatgcc gccgggcaga tggaaaacat cgagctgcgc gaccgttatc
    12301 ttgcggaaat cgccaaactg ccggaaaagc agcagctttc ccgttatctt ttgttggcgg
    12361 aatcggcgtt gaaccggcgc gattacgaag cggcggaagc caatcttcat gcggcggcga
    12421 agatgaatgc caaccttacg cgcctcgtgc gtctgcaact tcgttacgct ttcgacaggg
    12481 gcgacgcgtt gcaggttctg gcaaaaaccg aaaaactttc caaggcgggc gcgttgggca
    12541 aatcggaaat ggaacggtat caaaattggg cataccgccg ccagctggcg gatgctgccg
    12601 atgccgccgc tttgaaaacc tgcctgaagc ggattcccga cagcctcaaa aacggggaat
    12661 tgagcgtatc ggttgcggaa aagtacgaac gtttgggact gtatgccgat gcggtcaaat
    12721 gggtcaaaca gcattatccg cacaaccgcc gacccgaact tttggaagcc tttgtcgaaa
    12781 gcgtgcgctt tttgggcgaa cgcgatcagc agaaagccat cgattttgcc gatgcttggc
    12841 tgaaagaaca gcccgataat gcgcttctgc tgatgtatct cggtcggctc gcctacggcc
    12901 gcaaactttg gggcaaggca aaaggctacc ttgaagcgag cattgcatta aagccgagta
    12961 tttccgcgcg tttggttctg gcaaaggttt ttgacgaaac cggagaaccg cagaaggcgg
    13021 aggcgcagcg caacttggtt ttggcaagcg ttgccgagga aaaccgacct tccgccgaaa
    13081 cccattgaaa ccttcagacg gcatcgttga aatgccgtct gaaaaccatt tcacttgaca
    13141 aacagactga cagaaaaccc gtatgactct tttaaaaaac gataccttcc tccgcgccct
    13201 gcttaaacag cccgtcgaat acacgccgat ttggatgatg cgtcaggcgg ggcgttatct
    13261 gcccgaatac aaagccacac gcgcgaaagc gggcagcttc ctcgatttgt gcaaaaacac
    13321 cgaattggcg accgaagtta ccatccaacc tttggaacgt ttcgatttgg acgcggcgat
    13381 tttgttttcc gacatcctga ccgtccccga cgcaatgggc ttgggactgt attttgccga
    13441 aggcgaaggc ccgaaattca aacgcgccct gcaacacgag gacgacattg ccaaattgca
    13501 cgtccccgat atggaaaaac tgcaatacgt tttcgacgcg gtaacttcca tccgtaaagc
    13561 attggacggc cgcgtaccgc ttatcggctt ctccggcagt ccgttcacgc tcgcctgtta
    13621 catggtcgaa ggcggcagca gcaaagaatt ccgcaccatc aaaaccatga tgtactcgcg
    13681 ccccgatttg ctgcacaaaa tcctcgatac caacgcccaa gccgttaccg cctacctcaa
    13741 cgcccaaatc gacgcgggcg cgcaggcggt gcagattttc gacacttggg gcggcgtgtt
    13801 gagcgatgcg gcgtttaaag aattcagcct caaatacatc cgccagatcg tcgccggact
    13861 caaacgcgaa agcgaaggcc gccgcgtgcc tgttatcgtg tttgccaaag gcggcgggct
    13921 gtggctggaa agtatggccc aaatcggcgc agacgcattg ggcttggact ggacgtgtaa
    13981 catcggcgaa gcacgccgcc gcgtcggcaa gcaagtcgcc ctgcaaggca acttcgaccc
    14041 gttcgccctc ttcggtacgc cggaatccat ccgcaccgag gtcgcacgta tcctagccga
    14101 ctacggacac ggcagcggcc atgtcttcaa cctcggacac ggcatcaacc aacacgccga
    14161 ccccgaacac gccaaaatct tagtcgatac cgtacacgag ctgtctcggc agtatcacgg
    14221 cgggtaagcc ggcaggaaac cgcccgatat gccgtctgaa gccgagagat ggccggttag
    14281 ggtaaaaata aggcaatgcg gcaatatccg ccgtgtacgg atagtacatg acggcggcgt
    14341 tgtcgtattg gcgcaatccc aaccgtccct atgttcagac ggcatttttg ttttcagatg
    14401 cagggaaaac cgatggcaaa aacgcttaaa accctttacc aatgcaccga atgcggcggc
    14461 acttcgccga aatggcaagg caaatgcccg cattgcggcg agtggaacac gcttcaagaa
    14521 agcttagccg cgcccgagcc gaaaaactcc cgcttccaat cttgggcggc ggatacctcg
    14581 accgtccaat ccctctccgc cgttaccgct gccgaagtgc cgcgcaaccc gaccggcatg
    14641 agcgaactcg accgcgtatt gggcggcggt ttggtcgatg gcgcggtcat cctgctcggc
    14701 ggcgatcccg gcatcggcaa atccacgctg ttgttgcaaa ccatcgccaa aatggcgcaa
    14761 agccgcaaag tgctgtacgt ttccggcgag gaatccgccc aacaagtcgc cctgcgcgcg
    14821 cagcgtttgg aactgcccac cgaaggcgtg aacctgcttg ccgaaatccg catggaagcc
    14881 attcagacgg ccttgaaaca gcatcagccc gaagtcgtgg ttatcgattc catccaaacc
    14941 atgtattccg accaaatcac ttccgccccc ggctccgtgt cgcaggtgcg cgagtgtgca
    15001 gcccaactga cgcgcatggc gaaacagatg ggcatcgcca tgatactggt cggacacgtg
    15061 accaaagacg gcgcgattgc cggcccgcgc gtgctggaac acatggttga taccgtgctg
    15121 tatttcgagg gcgaccaaca ttccaactac cgcatgatac gcgccatcaa aaaccgcttc
    15181 ggcgcggcaa acgaactggg cgtgttcgcg atgacggaaa acggtttgaa aggtgtgtcc
    15241 aacccgtccg ccatcttcct tgccagctac cgcgacgaca cgcccggttc gtgcgttttg
    15301 gttacgcagg aaggcagccg accgcttttg gttgaaattc aggcattggt cgatgacgcg
    15361 cacggattca cccccaaacg ccttaccgtc ggactggaac aaaaccgcct cgcgatgctg
    15421 ctcgccgtgt taaaccgaca cggcggcatc gcctgtttcg atcaggatgt gttcctcaac
    15481 gccgtcggtg gcgtgaaaat cggcgaaccg gcggcggatt tggcagtcat cctcgcgatg
    15541 ctttccagct tccgcaaccg cccgctgccc gaaaaaatgg ttgcatttgg agaaatcggt
    15601 ttgagcggtg aagtccgccc cgtcgcgcgc ggacaagagc ggctcaaaga agcggaaaaa
    15661 ctcggcttca aacgcgccat cgtcccaaaa gccaatatgc cgcgcaacgt caaagagttt
    15721 ccgaacctga aaatctacgg cgtttcgagt ttgcaggaag ccatcgatat ttgccgcgac
    15781 agcagggaat aaacggaaat gccgtctgaa atcgggtttc agacggcatt tggtttgtgg
    15841 cggattgaaa caagaaggca taccggcgac agataagatt tgtggcaaag ttgcctgtga
    15901 tgtggcaaaa acacacacgc ccgtcatccc cgcgagggcg ggaatccgga atcgtccgtt
    15961 tcggcaatga ttgaaaatca cggcaatcca accgcttgga ttcccgactt cgcgggaatg
    16021 aggggcgtgt gcgtttgatt tccatccgcc atatgtcggc aacgggctta ttcgcctatg
    16081 gttttttgta tcagtttttc ggcgtttgcc aaagtgtttg ccacttcgtc gaaaccgatg
    16141 cggctgccgg cgatgagggc gcgcgtatcg gtataggcgg cgcgtacttt gccgtccgtt
    16201 tcggtaacga ggacgcgcag gggcagttgc agggcgaagg cggggtcttt gaccatcagc
    16261 ggcgtaccgg ctttgggcgt gccgaagacg atgacttttg ccggctgcat cgttaagccg
    16321 tttcggcggg cggcttcctg atggtcgatg acggcaaaaa tgtccatccc tttgcttttt
    16381 atggcggttt caaggcggct gacggtttcg tcaaaactgt attttgaggt gagggtatgc
    16441 gtggtcatag cggtttcgtt ttgggtttgc ggttcgctgg caggatgtgc cgaagcggtt
    16501 gaaatgcaga gtgcggatgc ggcaatcagg gggagtatgt gtttcatcgt atttcctttt
    16561 tccttttcgg tttgtttatc aggcttcgga cggggcggca cgcgccgcgc ccgatgttgc
    16621 gccgcgtgcc ggggtgtcgt atgccgtctg aaagcctgtc ctttcagacg gcattgcgtc
    16681 atttcatccc ttttttgagc aggtcttcat aaccgccgtg attggcaaca ttcgtatagc
    16741 ctgctttttt cagttcttga agggcggctt cggcacgccg tccgctgcgg cagtagaggt
    16801 tgaccggcgt gtctttgtcg ggcgcggctt cgtgtatgcg gcggacgatt tggtcgacgg
    16861 ggatgttgac cgcgttgtgc aaatgacctt cgctaaattc ctgttcgctg cggacatcga
    16921 tccaaacggc tgaatgttgc gcggtttggg cggcggatac gggtttttgc ggggctgcct
    16981 gcgcggcaaa ggcggctgag gcaatgagtg cggcggtaat caggtgtttg atattcatag
    17041 ggttctcctg cggttgtcgt cttaagggac gggaagttat tttatctgtt ccaaagcggc
    17101 ggcatctatg tcccaatgcc aaacgccgcc gcctttgccg tctatgccgc gtaaaaacag
    17161 gtagcggacg gaaacggcgg cgggcggttg tccgcgcagt ttgaagtagc gcgcggaggc
    17221 aacggcgtaa atcagtgcct gaaggtaata gtgatggtgc gcgacggctt cgtccatcgc
    17281 ctgccgcgtg taggcggatg cgtccgcgcc gagatggttt gatttgtagt cgatgacgca
    17341 gatatttccc tcggggtctt ggcagaccat atcgataaag ccgtttaaaa agccgttgac
    17401 ggtgtggaag tcgagcgttt cggcggcggc acggcagact tcgggcagcc tgatgtcgtc
    17461 gcgggcaaac cagtcgcgca gccgtttgag gccgaagtct tcggtgtgga gggtaaagcc
    17521 catttcggga cagcggcact cgggcgggat gtcggacagg cagtatgccc ccgtcagcgg
    17581 cgttttctgg caggcttccg ccatttcggc aacggcgggc agccacattt cttcaaaccc
    17641 gtatttcttc agcttgtcgg caatgagagt ttcctgtccg gcggctgctt gtccgaattt
    17701 gaaatcttca agaatttcgt gcaggcacag ccccgcctgc gtgcctttcg gaaaatcgtg
    17761 tatcgatatg ccgtctgaag ccgtcggcgt ttcagacggc atcgccgcta ccgaggtttc
    17821 ggcggcatcc aaggacgggc aggcatcttc ttcgccgccg tcgggcgttt gggtatggcg
    17881 gcttaaggcg gtaaagctgg tgtggcggac aaatcggaat ctgcgttcgg gaatgctgtt
    17941 tgcggcaaat tcggcggttt gaccggcgtt gctgcgatag gcggcagggg gcggcgcatt
    18001 ttcggtgaag gcgaaatccg tgccggccgg ggcgttgtcc gccacgcgcc gccagttttg
    18061 tttgagcatc gttatgccgt ccttttcttg ggcataggtt tggcggacgg tttcgcggct
    18121 gtcttgcggc aagccttcga ttagataggc gagggggttg tcggcggtat cggaagaata
    18181 ggcggcgtag atgttgagct gttcctcggc acgcgtcagc gcaacataaa gcaggcgcag
    18241 gcgttccgcc atttcttcat cggcgtattg cttctgttcg tcttccgaca gttgcgcctt
    18301 tgccaacagt tcggttcggt tggcgctttg gtggaggatt tgccagtcgg acggcccggt
    18361 atcttgcgcg tcccacgcaa acgggcagta caccagcgga tactgcaaac ctttcgaggc
    18421 gtgcatggta acgattttga ccaaatcttc gtcgctttcc agacggatgg cgcggttgtc
    18481 gccgctgttg ttgccggcca ggctgatttg gtcgcgcaac catttgtgca gcgcggcggg
    18541 gttgcggttt tgcgcgtctt cggcggcaag cagttcgagc agttggaaat agttggtcag
    18601 gctgcgcccg ttgttccggc ttaagaggcg cgtttcgatg ccgtgtgttt gggaaaattg
    18661 ctgcatggcg gcgaaaatgc cgtattgatt ccagttgtcg agcgcggttc gggcagattc
    18721 cgcccaatgc aaaatctcgc tttcgttttg gttgaagtcg tgcaattgct gcgcgttata
    18781 gccgaatatg ctgcttgtca ggacaaagcg cagcgttccg gcgcggcgcg gttcgagcca
    18841 gaagccgatg agtgcggaca gggcggcggc ttcgggcgag gcgaacacgg attcgcgcga
    18901 aagcaggacg ctttgcacct gccgtttttt cagggcggcg gaaaccatca ccgcctcgtt
    18961 gtgcgtgcgt accagcacgg caatatcgcc cgactgcaac gggcagcctt tgaaattcag
    19021 acggcctctg gcggcttcgt tgagcgcgtg ggcgatttcg tcggcgcaat agtcggcggc
    19081 acggcggcgc aaaacgtctt tgttggcttt ttcattgtcg ttttcgtgca gccaacgaac
    19141 ctgtacggca ggacgttcgg gggacagcct gctttcggca cgcgccgcac cgacttccga
    19201 atagccgatg ttttccaaaa cgaacgggcg ttctttgagg cggaacagcg cgcctatgct
    19261 gccgataagc gcggcgtggc tgcggtagtt ggtggcgagc gtgtagcggt gccgcgcgtc
    19321 ttccgccgcc tgaaggtagg cgtaaatgtc cgctccgcga aagctgtaaa tcgcctgttt
    19381 gggatcgccg acgaggaaca gcggtcggtt ttgggcgatg aaaatctttt ggaagatttc
    19441 gtattgcagc gggtcggtgt cttggaactc gtcgatcagc gcggtttccc agttttcggc
    19501 aacggcgcgg gcgagagtgt cggcgtgcgg attgtcggtc agcgcggtgt ggacatcgag
    19561 cagcaggtcg tcgaaaccgc gttcgcggcg cgattttttc atctcggcaa ggctgcggtt
    19621 gaggtattcg attaaatcca gttgcagccg gatcattgtt gcttcttccg cttcttcgag
    19681 tgcgttcaaa tcgcgcccga agtctgccag tttctgcaat tcggcaaata ctgccgcatc
    19741 gggcgttttg ccttttttca gtccggcttc gagtttgtcg gatgcaagtt tcaagagtct
    19801 gtcgtgtgtg tctttgtcca gaaagggcag ttgtccggcg gcggattttt gtgccagttc
    19861 tttaaaaagg ttgccgaagc tgtttttgcg gtaactgttg ccgttgaggt cgggatgaat
    19921 gcgccaaaag ccggcttcca gttctggcag caggcggcag atggtttgcc atgaggtttc
    19981 ggcgttgcgc tgcgcctgtt tcaaatccgc ctgcggacgg cggaaattca ggtacgggcg
    20041 ggaaagatag cgcgaaattt gggcaaggac ggtttgcggc acagctttgc gtttaagcgc
    20101 caatgcggca agcaccggat cattgctgac gcgttcccgc caaaaatctt gcgccgggat
    20161 aagcaggcgg tcgccgtctt cttcggtcat ttcgacatcg aacggtgctt ggcacaggaa
    20221 ggcgtagtcg cgcaggatgc gctggcagaa gccgtggatg gtatagatgg cggcgttgtc
    20281 gaattgcccg atggcggcct tgaggcggac aatcagacgc gtccggccct ctttttgcaa
    20341 agcctgtttt aagagttcgg gcaggaaggt gtcgccttcg tggtgttcgg cgcagtaggc
    20401 ggcaatgccg tctgaaagcg tgtcgtctcc aagtttggca attcctttgc tttctaaaac
    20461 ttgtaacaca tcgtccaaac gcccgcgcag gcgtgttttc agctcggcgg tggcggcttt
    20521 ggtgaaggtt acgaccaata cgcgttcgac gtttttttgt tctaatacga tcaggcgtgt
    20581 aaacagggcg gcaatgccgt aggttttgcc ggtgccggca gaggcctcaa tcaggttggt
    20641 gccggaaatg gggacggtta gcgggtcgaa tgcttggatg cttgcagaca tagtgcgcgc
    20701 tcggaaaacg gttggacggt atagtggatt aacaaaaatc aggacaaggc gacgaagccg
    20761 cagacagt
//
LOCUS       DAWKQB010000020        20712 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.020, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000020 DAWKQB010000000
VERSION     DAWKQB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.020"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(63..239)
                     /locus_tag="WJL14_000568"
     CDS             complement(63..239)
                     /locus_tag="WJL14_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655682.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(242..1600)
                     /locus_tag="WJL14_000569"
     CDS             complement(242..1600)
                     /locus_tag="WJL14_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ7655683.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQMVAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1724..3371)
                     /locus_tag="WJL14_000570"
                     /pseudo
     CDS             complement(1724..3371)
                     /locus_tag="WJL14_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(3394..4872)
                     /locus_tag="WJL14_000571"
     CDS             complement(3394..4872)
                     /locus_tag="WJL14_000571"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ7655684.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCHTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRLLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4897..6642)
                     /locus_tag="WJL14_000572"
     CDS             complement(4897..6642)
                     /locus_tag="WJL14_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ7655685.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIT
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WJL14_000573"
     CDS             complement(6703..6966)
                     /gene="ftsL"
                     /locus_tag="WJL14_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ7655686.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WJL14_000574"
     CDS             complement(6963..7949)
                     /gene="rsmH"
                     /locus_tag="WJL14_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ7655687.1"
                     /translation="MSGAESYRHITVLLDEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WJL14_000575"
     CDS             complement(7946..8401)
                     /gene="mraZ"
                     /locus_tag="WJL14_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ7655688.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8680..9897
                     /locus_tag="WJL14_000576"
     CDS             8680..9897
                     /locus_tag="WJL14_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7655689.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            10029..10105
                     /locus_tag="WJL14_000577"
     tRNA            10029..10105
                     /locus_tag="WJL14_000577"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10063..10065,aa:Met,seq:cat)
     gene            10113..10188
                     /locus_tag="WJL14_000578"
     tRNA            10113..10188
                     /locus_tag="WJL14_000578"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10146..10148,aa:Ala,seq:ggc)
     gene            complement(10479..11300)
                     /locus_tag="WJL14_000579"
     CDS             complement(10479..11300)
                     /locus_tag="WJL14_000579"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ7655690.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11333..11977)
                     /locus_tag="WJL14_000580"
     CDS             complement(11333..11977)
                     /locus_tag="WJL14_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7655691.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYGIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11980..12843)
                     /gene="ftsN"
                     /locus_tag="WJL14_000581"
     CDS             complement(11980..12843)
                     /gene="ftsN"
                     /locus_tag="WJL14_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7655692.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12949..14445)
                     /locus_tag="WJL14_000582"
     CDS             complement(12949..14445)
                     /locus_tag="WJL14_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ7655693.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHLVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14459..14779)
                     /locus_tag="WJL14_000583"
     CDS             complement(14459..14779)
                     /locus_tag="WJL14_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ7655694.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15261..16787
                     /gene="putP"
                     /locus_tag="WJL14_000584"
     CDS             15261..16787
                     /gene="putP"
                     /locus_tag="WJL14_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ7655695.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKAYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWI
                     KKPAQAAGESGLLTMYEIVPGFIVCLIIAVLVSLFNKEPSREIQERFEKADADYRAAR
                     "
     gene            complement(16877..17044)
                     /locus_tag="WJL14_000585"
     CDS             complement(16877..17044)
                     /locus_tag="WJL14_000585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655696.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            17036..20641
                     /gene="putA"
                     /locus_tag="WJL14_000586"
     CDS             17036..20641
                     /gene="putA"
                     /locus_tag="WJL14_000586"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ7655697.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     SVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4845 a         5981 c         5049 g         4837 t
ORIGIN      
        1 cgggcctgcg cccgtttgag gttggcttgc cgcccggcaa ggtttcggac ggcttttgca
       61 gattaacgtg cagggacttt ctcaatcacg caaatcgggt aactccccgg atttttgccg
      121 ccacccaagt ctaaacataa atcttcataa agatacgcct gcgtctgcat tccggcataa
      181 aacgcgccga ccagcgcggc aatcaacaat aaggctttaa aaaaacgtcg acttttcatt
      241 ttcacttatc ctccaatgcc tcgaccactt cttccatctg cataaagcgc gaacctttca
      301 ccaacacggt ggcgcgttcg ggcaaatcgt ggcgcaacac ttgaatcaac gggtctttgg
      361 cggcgaacca caaaccgtcc gcgccaaatt tttccgccgc ttcgacgctg ttgtcgccga
      421 caaaataagc cgcttcgatg ccttggtcgc gagcatacgc gccgacttcg gcgtgcatgg
      481 cggcggcttc gtcctcaccc agttcgccca tatcgcccat cacgaaaata cgcggcgcag
      541 gcatacgcgc caacacgtca atcgcagctt tcatgctgtc agggttcgca ttataagtat
      601 cgtcaatcag ggttgcgccc ttgattccgg atttgacgtt cagacggcct ttgatattgc
      661 tgaagccttt caaaccttcc gccacatcgt tcaaactcaa acctgcagcc aaagacagcg
      721 cggcggcagc ggcggcgttg tggacattgt ggcggccggg aacaggcagc accacggcgg
      781 cgcgttcgtc gccgcacacc aaatcaaatt cgcacgacaa cggtttcagc acgatatttt
      841 ccgcgtggac atcgccgcta tcgatgccga aagtgcgcgt attcaaatta agcgttgccg
      901 ttttgaagac agccatattg gcatcttctt gaggaatcag tgccatgccg tctgaacata
      961 agccttgata aatctcgctt ttcgctttgg caatatcgcc cactccgtcg aaaccgcagc
     1021 cgacatgggc gcgcatggcg ttgttgacca atgcggcatc gggtttggcg atttgtgtca
     1081 aaaccgccag ttcgccaaaa tggttcatac ccatttcaat cacggcatag cggtgttttt
     1141 cgtttaattt caacaaagtc aacggcaatc cgatgtggtt gttgaagttg cctgccgtcg
     1201 ccaaaacggc attatcgccg aaacggcggc gcaataccgc agccaacatt tccttcaccg
     1261 tcgtcttacc gcccgaaccg gtaataccga acacaaacgg gttcacattc tcgcgccacg
     1321 ccttcgccac catttgcaat gcggcaagcg tgtcatcgac tttcaacgcg ccatccattg
     1381 caacgcaatc ttcgcgcgaa accacaaccg ccgccgcacc agcagccaat acgtcttcaa
     1441 caaaatcatg cgcatcaaac cgcccgcccg ccaatgcgaa aaacacatcg cccgcgcgga
     1501 tgtcgcggct gtcggttacg atgcgcgaca cgggtttgct ttcagacggc atcggaagct
     1561 tgagggcttg gcagatgaaa tttaggtcca gtggtttcat atttactttc gttaatattc
     1621 gggcggcgga cacatcggta gcggctgatt ttttatcgcc tgttttactg tggtaaaaca
     1681 cagattattt tcccattctc attcggcatt ttttctgtac gtatcatttt ttagacgtat
     1741 ttttagtcga tttgcctttt cccgcatacc acggcgcggg gtcgtcgggc agtccgtcga
     1801 taaaggcaag gttattgcct tcgccctgca catcgggaac attcccccaa aaatcatagc
     1861 cgtcatcggg caactcgtcg gtttcgatac ccgtccaact gccgaatccg cgtaaaaaat
     1921 taaacgcgct gcgcctcact ttaatcattt cgcgccgcgt gtcatcggac gaaattttaa
     1981 ccagcggcac gccgtagctt tgacgcttcc acgcaccatg tgtcaacgtt cgctcgccgc
     2041 ctttaccgac atgcatcaaa ccgtggtcgg aaaagtaaac caaagaccag ctttctttat
     2101 tttcattcaa tatcttaact gtatcttcta aaaatttatc ggtttgcgcg atggtggaaa
     2161 catagcagga tattttttca gtttgatact gaaaccgccg cgcatcctta tccaaacgtg
     2221 tgcaaaaatc actgtgcgaa cccatcaggt gcatcacaat cagccgaggc ttcgtgcctg
     2281 catttcctat caaaacccgt ttgaacgccg gcaacaaaag gcggtcgctc aaccccgcgc
     2341 ttttgccata atcacccctt tgggtaaacc acggataatc gctgcgtagg gcataggtgg
     2401 aaatttcgtt ggcaaaatgc cccaacattc cttgattaga cagccacgcc gtccgaaaac
     2461 ccgcctgctt cgccaagctg acgatgttat tgttcggttc tcccggcagc cccaaagtct
     2521 gcggcagcga aagattcgtc gcgtgggcgg tcgattggta accgtttatc aacagccctt
     2581 tggtctgact caaaaaaggc gtatcgggca atgggaaacc gtaaacattc atataatccg
     2641 aacgcgcgct ctcaccgata accacaacat aatttttata cttgggcgca acatgacgga
     2701 tatgccatgt tgacgctttt tttgctgctt ccaaaatgtg ggcgcgcttg gcggcatatt
     2761 gtgccggcgc ggaagccaaa tcgtaataca ggtcgaaaat attcaacaac aggccggcat
     2821 caggttctcg caaatcttta tcgctggcga ttttatccat caccgcgcag gacaaaatca
     2881 aagtcaataa tatagtcagc catattttgc tgcggcgttt atagtttttt acgtcagcaa
     2941 ataccccccc ccgatacaca atatctccaa actgtcaagc caaaaatgaa aaataatgcc
     3001 tgcacaaaat aaagcgaccc gggaagattg ccgacaaatt cacgcgcctc ggcaggattg
     3061 ctttccaata tcgaaccgac tatctgataa gacggcgcac catacagcca gccgaccggc
     3121 aaatataggg cggttgtgcc gacgtaaatc agtaaaacaa ctgaagaaac gcgcgggaaa
     3181 acgcgtgcca ataataataa aaatacaacc gaagccaaag cgcccacggc agaataacgg
     3241 tagccgtatt catattccaa gtgatagcct gatgcgatgg ctgcggcaaa acaaaaggct
     3301 aaaagcgcgc aaatgcccaa tgtatttttt aatgtctgat tcatattttt tattcgggcg
     3361 gattgtttca tacgatgccg tccgaatatc ccattattta cgagttaaca aagcctgtcc
     3421 gacgatttca agatcggaaa aacggtgctt cacgccttgt acatcctgat agttttcatg
     3481 ccctttgccg gcaatcagga tgatgtcgtt tgcggcggct tgttcaaccg cataacggat
     3541 ggcggcggca cggtcggctt cgacgcattc gggcgcggga acggcaggca ggatgtcgtt
     3601 gatgatgtcg tgcggatttt ccaaacgcgg attgtcgctg gtgacgacga ctttatccgc
     3661 gccctgtacg gctgccgcgc ccatcagcag gcgtttgccg cgatcgcggt taccgccgca
     3721 accgaatacg caccacaaag ccgcaccctg cggtttgatt tcctgcaagg tggcgagtgc
     3781 tttttccaat gcgtcgggcg tgtgggcata atcgacaacg accaaaggtt tgccgctgtt
     3841 cataatacaa tccatacggc ctgaagcagg acggattttt tccagtacat ccaatacctt
     3901 atccagcgga tagccgttgg cgcaaagcaa agcgacacag gcggcgagat tttgcgcgtt
     3961 gaaccgtccg agcaggcgcg tgtggcattt cccttcgccc cacggggttc ggaatacggc
     4021 ttctatgccg tctgaagagg cggtaaagtc ggtaatgcgg atgtcggcgt gttcgctgaa
     4081 gccgtaactg taaacggcca aatcggggca gtcttttttc agacggccta tgagttctgc
     4141 gccgtatccg tcatccacgt tgatgactgc gtgtttcaag ccgtgccagt aaaacaggcg
     4201 cgacttgatg gcaccgtagg cttccatcgt gccgtggtag tcgaggtggt cgcgggtgag
     4261 gttggtaaag attgcgctgc ggaatgacac gccgttgacg cgcgactggt caagcccgtg
     4321 gctggagact tccatcgcgg cgactgttgc gccttgttga cggaaacggt agagcagggt
     4381 ttggacatcg acgggggcgg gtgtggtatg cgtggtttct tccaatgcac cccaaaagcc
     4441 gttgccgacc gtgccgacaa tggcggtttt ttcgcccaac aaatcggcag cttgcgccag
     4501 ccattgcgtg atggaggttt tgccgttggt tccggttacg ccccagaatt tgagaccgtc
     4561 tgaaacgttg ccgtaaactt gcgccgccaa tatgccggca cggtgtttca aatctttgat
     4621 gccttgattg gggactttcc attcgggatt ccacgcaaat ttgccgtcgt cgtcccaaaa
     4681 aacaaaagcc gcgccgttgg caacggcggc ggggatataa ctgcgtccgt cggcatattc
     4741 gcccggacag gcaacgaaaa tatcgccttg tttgatttgg cggctgtctg aatgcaacaa
     4801 acgccctgcc gcgtttgcac acgacagggt cgggatgccg gtttcagcca aaggggttaa
     4861 cttgctgaac ataaaacaat ctcgttgata ctcggattaa gacggtgttt tgacggctgc
     4921 ggcggtcagg ggcttggtcg gggaaatgcc caagatgttc agactgccgc ccataatttt
     4981 tttgaagggc ggccctgcca ctacgccgcc gtaataaccg tgggcagtcg gttcgtcaat
     5041 ggttaccgcc acaatcacac gggggttttt ggcgggggca aaaccgataa aggtagcgat
     5101 gtgtttgttg tcggcataac gcccgttgac gaacttgcgc gccgtgccgg ttttcgcgcc
     5161 gacatcgaaa ccgtccaccg cacccgccgt accggtgccg cccggctcgg ttacggaaac
     5221 catcagattg cgtacctcgc gcgcggtcga ttctttgaat atgcgtttgc cttgcggcgc
     5281 aaccgcctgt ttttcaaagc tgaccggcag taaaacgccg tcgtgcgtca gtgcggtata
     5341 ggcgcgcgcc aattgcagca ggctcaattg caggccgtaa ccgaaagaca tcgtcgcctg
     5401 ttcaataggc cgccacctgc gccaatttct caacaaacct gcagtttcgc ccggaaagcc
     5461 cgagtgcata cgcacaccga tgcccaactc atgatagaag tcatacattt cttcggcacc
     5521 gaaacgcgca gacagtttgc ttgtgccgac gttggacgat ttctgcatga tgccgcgcac
     5581 atccaaagag gggtaaacat gggtatcgcg cacgggagac ggtccgattt tataaggctg
     5641 cgtattcagc cgttcgttca aatcggtttt gcccgcatcc aatgctttcg caatcacaaa
     5701 cggtttgatt gccgaaccgg gttcgatcat atcggttacg gcacggttgc gccgctgttc
     5761 gctgtctgcc cggccgggcc tgttgggatc gtaggcgggc gtattggcca aggcgaggat
     5821 ttcccccgtg cgggcatcca aaaccaccac cgttccggct tttgcctgat ggtattcgac
     5881 cgccttgttc aactcttcat aggccaaggt ctgaatcctc tgatcgaggg aaaggatgat
     5941 gtctttgccg tttttcgggg ctttattgcg cggggagtcc aagctgtcca caatattgcc
     6001 ctgccggtcc cgcaaaacga cttccgcgcc gtcttcgcca tgcaggctgt cttcaagcga
     6061 aagttccaaa ccttcctgac ctttgccgtc aatatcggta aatccgatga cgtgtgcaaa
     6121 caggttgccc atcgggtaat ggcgttttaa ttctttttca aatacaaagt tttccaaacc
     6181 caaggctttg acctcttcgg caaccttggg atcgagctgc cgcttaatcc agataaacga
     6241 cttgcctttc tgttcgagct tgttcctcaa aacatcaacc ggcacatcga caagctcgga
     6301 caggcgttcc aattgtgcgg cagacggcat ttccttcatc tctttaggca cggcaaacag
     6361 ggactccgtc ggcgcactca acgccaaaac cgcaccgttc cggtccgaaa ccgtaccgcg
     6421 tgtagccggc aatgtttgag tccgcacaat ccggttgtcg ccctgttctt tcaaaaagtt
     6481 atacgttacc gtctgcagat acagtccgcg agtaatcaga ccggcaaaca agaccgctat
     6541 tgccatcagg acgaagctga tccgtccgtt actggtcatc ggctttttga cctgctcttc
     6601 tttgggcagc atccgaggct tatattcgct cttaatcaac atttttactt ctcgttatta
     6661 ttatcctgac gcaggaatcc gattccggca cacaggctgc ttctatcttt gatgcgccac
     6721 cataaaggta ttgcccgaaa ccggcggatg gaggttttgt ttttctgccg ccgccctgat
     6781 cgcttcgtgg ttcgccaaac gcgcctgttg cagcctcatt tgcgcataat cctgctccaa
     6841 ggcgatttcc tgttttttcg ccttgtccaa agctgtgaag ttgagcctgt actggttttg
     6901 ctgcatcaca acggaaaaag cggaaacgca caccgcaagc agcagaagga aattcaattt
     6961 gttcattgcc attcagacgt ttttctctgt gattgttccg gtatcgaacc ggcagtccgc
     7021 tccgccacac gcaaaaccgc acttctcgcc ctcggattgg cggcaatttc cgcctcaccc
     7081 ggctttaatg ccctgcccac gattttcagg ggcggctcgg gcaaatccgc ttccctgacc
     7141 gccgcccagc gcggcagggg cgcgtgttgc gaatattttt tgacaaactg cttcacaatg
     7201 cggtcttcca acgaatggaa agcaatgacc gccaaacgtc cgccctcttt cagacgacac
     7261 atgacctgcg gcaatactgc ccctacttct tcaagctcgc ggttaataaa gatgcggacc
     7321 gcctggaagg tgcgcgtcgc aggatcctgc ccccgctcgc gagtacggac gttttgtgcc
     7381 acgatctgcg ccagcttgcg ggttgtatcg attggacttt ccgcccgttg cgcaacaatg
     7441 gcgcgcgcaa tctggcggct aaaccgctct tcaccataat tcttgattac ctcgtgcaaa
     7501 tcctgttccg acgcaaccgc tatccactct gcggcagaca taccgcgcgt cgtatccata
     7561 cgcatatcca aaggggcatc gaaacggaag ctgaaaccgc ggctgccgtc atcgatttgc
     7621 ggggacgaaa tccccaaatc aaacagcgca ccgtccacct tgccgatacc caaaccgtcc
     7681 aatgccgtct gaaacgaagc aaaaccgcca tgcacgacac cgacccgttt gtccgaacgc
     7741 gccagctctt ctgccacagc aatcgcctga gggtctttgt cgaaaacaat caaccgcccc
     7801 gcatcgccca aacgcgacaa aatcagccgg gaatgccctc ccctgccgaa cgtaccgtcc
     7861 acatagacac cgtcttcgcg cacggcaagc gcatccaccg cctcatccag caagaccgtg
     7921 atatgccggt aactttctgc tccactcaca attgcaaatc cgtctgactc aactggaagg
     7981 caagttcgtc aggatcgtca tccaaagcct gaaccatttc ggcctcccac tgctcacgac
     8041 cccaaagctc caaacggttg gcacgaccga ccaaaacgac ttcacggtcg aaatccaccc
     8101 ttttcctcag tccggcagga agcagcaccc ggccggcgct gtcccattct aaaatttccg
     8161 cgttatgcag caaaagattt tgaaaccgcc gcaaaacagg gttatccgcc acttttaagt
     8221 ttaaaagttg cgccgcaacc ttttcccact ccgcaacagg gtacatcaac agcttgtgtt
     8281 tcgactcgag cgttaccact acggcaggcg tatagaggcg cgacagaatg tcacggaatt
     8341 tggcaggaac agccaaccgc cccttactgt cgatgcttaa ttcgtgtgcg ccgccgaaca
     8401 tgacatgtcc caagccgaaa tcaaaatcac aagggtaaaa gagacacttt gccccacaat
     8461 tccccaccaa tcgacactat aagaaatttt aaacactcgg tcaaatcagg gcatgaaaac
     8521 tcattaacat atctgaaatt tttattcctt ttaaaaacaa taagataaaa aatgacgaca
     8581 acggcacggc gggtgcggta cagagtgacc gaaccaataa acaactatat atgattaatt
     8641 taataatata aacacaatat atagtattaa gataaagcca tgacagcacc cgtaccaacg
     8701 tgtaatatgt cgggaaatcc aataaattta cacaagctaa cacttatcat gcccctccct
     8761 ctcccctctc ccgaagcacg gcaatcctcg ctcaaactgc aaaccctcat tgccgaaaaa
     8821 atcggcaaac acggcaactg gattccattt tcacgtttta tggaattggt tttatacgct
     8881 ccgcaatacg gctactacac cggcggcagc cataaaatcg gcaataccgg ggattttatt
     8941 actgcaccga ccctcacctc tctgtttgca cagacactgg cacgccaact tcaagaactt
     9001 ctatcccaaa cggcgggcaa tatctatgaa ttcggcgcgg gaaccggaca gcttgccgcc
     9061 gatttgttgg gcagcatttc ggacggcatc agccgttact atattattga aatatcgccg
     9121 gagctggcag cacgtcagaa aaacctgatt caagcacgcg caccggaagc atctcaaaaa
     9181 gttgtccact tgaccgcact tcccgaagcg tttgacggca tcatcatcgg caacgaagta
     9241 ctcgatgcca tgcctgtcga aatcgtccgt aaaaatgaag gcggctcatt cgagcatgtc
     9301 ggcgtttgcc tagataatga ccgttttacc tattcggcac gaccgctgca cgacttacag
     9361 ctatctgcct tggcttccct ctattttcct caaacagatt atccctacac cagcgaacta
     9421 catccgcaac aatatgcctt tatccgcacc cttgcctcaa ggcttgaacg cgggggtatg
     9481 atattcatcg actacggttt tgatgcagcg cagtattacc accctcaacg caatcaaggt
     9541 actctgatcg gacactaccg acatcacgtt atccacaatc cttttgactt catcgggttg
     9601 gccgacctga ccgcgcatgt caactttacc gacattgccc aagccggtac ggatgcagga
     9661 ctggatttga caggctatct gccccagtcc cattttctgc tgaatctggg cataaccgaa
     9721 ctgttggcac aagtgggaaa aacagactcg cccgcctaca tccgtgaagc tgctgccgtt
     9781 cagaaactga ttgaccagca tgaaatgggc gaactgttta aagtcatcgc attcggaaaa
     9841 aatatcggcg tcgactgggc aggattccgc ttcggcgaca tctgccacaa actctaaccc
     9901 tcatgccgcc tgaatccgct tcagacgaca taaacttttt aacatttaaa aacagtcaac
     9961 taattcaaaa ttaaaaaata cggcttgtca aaaaaacaga aaaacatata atagcgtctt
    10021 cacgaaacgg cgaattagct cagtcggtta gagcagagga atcataatcc ttgtgtccgg
    10081 ggttcgagtc cctgattcgc caccaaattt tcgggggtat agctcagttg gtagagcgct
    10141 tgcatggcat gcaagaggtc agcggttcga tcccgcttac ctccaccaga taaaaaagca
    10201 cagaccgtaa aaaggtatgt gcttttttat tgcctgattg ccggcaaata aagaataaac
    10261 cactgccttc aaaacaggca atcgacttta aacctatcgc cccgcctgtc ctgattttat
    10321 agtgaattaa atttaaaccg gtacagcgtt ggctcacctt gccgtactat ctgtactgtc
    10381 tgcggcttcg tcgccttgtc ctgatttttg ttaattcact atatcagccc gccagacaaa
    10441 cccgacccga ataatgtctt caggtcgggt ttatggtttc attcccaact tatccagcct
    10501 gacagccaca atataatgat ggcaatacca aaaacaatgc ggtaataggc aaaaggaata
    10561 taatttttct tggaaacaaa cctcagcaac gcttttaccg ctaccaagcc tgaaacaaag
    10621 gcagcaacaa agccaatcaa aatcaaaccg acatcatgca gggtgaaaaa ccggtaatgc
    10681 ttcaggacat cataagccgt tgcggcaacc atcatcggca cagccaagaa aaacgagaat
    10741 tctgtcgcag ttttccgttc gattccccaa agcatcccgc ccataatcgt actgcccgaa
    10801 cgggacgtac ccggaatcag tgcaaacact tgggcaacgc cgatcatcaa ggcatcaatc
    10861 ggacgcaatg catcaacatc ggcaatttta ggctctgctc ggctttggcg tttctccacc
    10921 cacaaaataa aaaaaccgcc caaaaccagc atgactgcaa cactcaaggg gttaaacaga
    10981 tactctttga tttgtttgcc gaacaacagc cccatcacgg cggcaggtat aaaagcaatg
    11041 gcaagattga ggacgaagcg gttggctttc cggtcttttc ccacgccatg caacacattg
    11101 ctgaaacgtt gccggtattc aaacactacc gccaaaaccg caccgagctg gatggcaatt
    11161 tcaaaaacct tgtgattgct gtgaaaacca atcagattgc cgaacacaat caaatgcccg
    11221 gtgctggaaa tcggtaaaaa ttcggtaaaa ccttctacca agcccatcat cagggctttc
    11281 aggacaatca gaaaatccat tgcttgcgct tctttcggat acgggagttc ggctatttct
    11341 gtacagcagg ggtctgacgc ttgcgttctt ccctgacttt ggcaaccaat tctttaatcg
    11401 tatgaacgcc gccgtcaaag ccgttattga agataacgcg gtattttccg ccgacaataa
    11461 cggtcggcgt gctgtcaata ccgtattgtt ccgtcagttt ctgcattttt aatgcggcgg
    11521 cggcagcttc gggagaatca taggcgcgca tcagtttttt gccgtcaaag cctttttgag
    11581 acaaagccca ctttccggca accgacctgt tttccaagcg gattttttgt tcgtaaactg
    11641 ctttaaacac agcagggttt gcctgatatt tcaaacccga caaattgacg gcagccgcca
    11701 tcctggccag accgagcatt tcaggctgcc agaccacgtg ctccgtcctc aaataggcat
    11761 cagacggcaa tgccttgccc aatttcaata acaaaggatc gaaatgatgg caatgtacgc
    11821 agaaatagcc gaaaaattcc aaaacctcaa ttttgcccga ctgttcttga ggaatgggtt
    11881 tatccaacac aagatagtct tccctttccg tcagggcata tgcctgcgcg gacaacactg
    11941 ccgacagcag cagcggcaac agatgtttga gcttcataat tatttgcttt cgatagaacg
    12001 gatcaggctg gcgacttcat gttttttcaa ctcgtcctgc atttttttca ccgcatcggc
    12061 agacatattg ccgctttgca cccggtaaag cgttttatgt cccgcctgat aaccgaccac
    12121 cttggaagat atgcccaaga ttgccagttt ggcacgctgt ccttccgcgc tccggcggtc
    12181 ggcatacgcg cccatttgca gataatgcgt tgcttccgcc ttgtcgggcg ttttcatttt
    12241 ctgcacttct ttggcagcgg cactgcgcgc tttttcgatg ctgccgctgt tgaggatttg
    12301 ttccggggtc ggtttgggtg caactttttc cttttccgcc tttttctctt cttttgaagc
    12361 ttttttctct gtttctttag acggttttac cgcttgtttt ttaaccgttt cggcatcttt
    12421 cttctgcgct ttttccccga cggtttgttc acgctcttcc gtcagtgctt tcttgcgcac
    12481 tgcctgtccg tccgactttt cccgctccgg ctcgtccgcc ttttcctcaa cctcgtcggc
    12541 tttgtcggca acgggctgct tgtcggcagc tttttccgca tccgactgct ctgcctcttt
    12601 cgcagcatcc ggttcggaca aggcgttttg atcggccggt tcaggttgga tgtcttcctt
    12661 aggctggttt ttcggtttca ggatttccgt ttctgcaggc tgcttcgacg aagccgggat
    12721 tttgaacgca ttttgaccgc tttggttcag ataaaacaaa ataccggcaa taatgaccgt
    12781 cgccagtatc aaaccgaaga aaaaaccgga cagacctttt ccggattggg aaaatttgtt
    12841 cataaacata ccttaatgtg tttcagacgg cattaacgcc gtttgcttac gggcggatat
    12901 tctaacaata tcgccatatt tgggcaaaac ctgcttccat tcccgttcct ataaagcgcg
    12961 acggaaacct atggctttca tgacgtggct tctgccgact tcttcgtcgc ccgccaaatc
    13021 cgccaatgta cgcgccacgc gcataatgcg gtggaagctg cgggcggaaa gggagagttt
    13081 ttccagcagg ccgcccaatg cttcctgcgc ttctttttga atgcgggcgg atgtgtcgag
    13141 ttcactgaca ctcaaggcgg cattcacttt gccttgccgt gcgtattgtt tgtctctagc
    13201 ggcgataacg cgttccaaaa cggacgcgct gctttcccct gcttcctgct gcatcagttc
    13261 ggcggcggac aggctcggga cttcgatggt caaatcgatg cggtcaagca acgggccgga
    13321 aattttgctg cggtaacgcg cgacgctttc gggtgtgcag cggcagggtt tgacgagatg
    13381 cccgagataa ccgcacgggc aggggttcat ggcggcaaca agttggaatt tcgccggata
    13441 gacggcttgg cgcgccgcgc gggaaatgtg gatttcgccg ttttccaacg gttcgcgcaa
    13501 aacttctaaa actttgcggt cgaactcggg cagctcgtcc aaaaacaata cgccgtggtg
    13561 cgccaatgaa atctcgcccg gacgcggatc cgaaccgccg ccgaccatag ccgccgcact
    13621 ggcgctgtga tgcggactgc ggaaaggacg gtggctgtcg agttgttgct ggtggttggg
    13681 caggagcgaa cgcaatgccc aaacttctac caattcgtct tcggtcagcg gcggcaggat
    13741 gccgggcagc cgttgggaga gcatagactt gcccgttccc ggcggaccca tcatcaagag
    13801 gctgtgtccg cctgcggcag cgatttccaa agcaaggcgc gcggtgtgct gacctttcac
    13861 atcgcacaaa tcaggttgtc cgccatgttc aaacggcatc tgaggaactt ggcattcggt
    13921 ttgcgccaaa ggttcgatgc cgttcaaatg ggcggcgact tcgcccaaag agcgcgcgcc
    13981 gtaaacggta atgccgcgca tcacggcggc ttgtcctgcg ttttcttcag gcaaaacaaa
    14041 tgcacgtttt gcctgcatac cctgccacgc catcgccaac gcgccgcgca cggggcgcaa
    14101 cagccccgac agtgccaatt cccccgcaaa ctcgtattcc gccagttttt cgggcgcaac
    14161 ctgccccgat gcggcaagga tgccgattgc aatcggcaaa tcgaaacgcc ccgactcttt
    14221 gggcaggtcg gcgggggcga ggttgacggt aatttttttg gcggggaatt caaaaccgct
    14281 ttgaataatg gcggcacgga cgcggtcgcg gctttccttt acttccgtat cgggcagccc
    14341 gacgatgttg aaatgcggca ggccgttggc aaggtgggct tccacttcga ccaacggcgc
    14401 attcataccg ctcaaggcgc ggctgtaaac caaggcaagc gacatatttc agacggcctt
    14461 attcgccggc ttcggtttgc tgcctgattt cggcgacggc ttcttcggca gcggcttcag
    14521 ccgcttccaa tgctgcccgt tcgggatttt gcgcggcttc gagttttgcc aaacgcgctt
    14581 ccaaagccgc cagtttggta cgggttttga ttaaaacctg ctgctggatg tcgaattctt
    14641 cgcgcgtaac cagatccata cggttgaacg cgccgcccag catcgcctta atattttttt
    14701 ccacatcttt ggcagggctg ttggcgatgg tttcgctgat tttcgagccg acttcctcaa
    14761 aaagctgctt gccgaacata atctgtatcc ttcctgaaca tatcaaattc aatcggctat
    14821 tgtataagga aaaatgccgt ctgaaaacgg gcggcggata atcggcaaaa cataccgcgc
    14881 ctcctttgcg gttgctaaat tacacctcaa aacacctgcc gcctgaaaac ggatttcata
    14941 ttccgcgcaa cgcccgatta caagacacta caaaacaata tgctgtttta aatgattttt
    15001 ccgacgcgca tcgtttcaaa ctcggctttt taagccatta agtgctttgc aaacaacaga
    15061 aattgggtta tgctgaaacg gattatttac aatttcatat agttttatta catatcttat
    15121 tgtgattgaa gataatttat ccgaatcccc tttcgggtgt ccggattttc cgttgtactt
    15181 ttattagaaa aacatttcag gctcaagttg cttgcaattt caaagcctgt aattctgaag
    15241 ttttagacga ggaatagcac atgaatccca tgtacatcac ttttgcaatc tatttggttg
    15301 cagtccttct catcgggctt gccgcttatt tttccacgcg caatttcgac gattatattt
    15361 tgggcgggcg cagcctaggc ccgtttgtta ccgcgatgtc ggcaggcgcg tccgatatgt
    15421 ccggctggct gctgatgggt ctgccgggcg cgatttacct gagcggtttg aatgaggctt
    15481 ggattgccat cggcctcttg gtcggcgcgt atttcaactg gctcttggtg gcgggccgcc
    15541 tgcgcgtgca taccgaatac gccaacaatg cgctgacgct gccggattat ttcttccacc
    15601 gcttcggcgc gggcggacac ttgatgaaag tggtttccgc actgattatc ctgttcttct
    15661 tcacgattta ttgcgcctcg ggcattgtgg cgggcgcaac cctgttccaa agcctgtttg
    15721 aaggtatgac ttacaatcag gcaatgtggc tgggcgcggg cgcgaccatc gcctatacct
    15781 tcttgggcgg cttcttggcg gtcagctgga cggatacgct gcaggcttct ttaatgattt
    15841 tcgccctgat tttaacgccg gtgatggtct atctgggctt gggcggcgcg gaacagatgt
    15901 ctgccgcgat tcaaagcgtc gccgcaggca cgggcaaagc atacggcagc ctgtttgccg
    15961 gtacgaccgt catcggcatc atttccaccg ccgcatgggg cttgggctat ttcggacagc
    16021 cgcacatttt ggcgcgcttt atggcggccg aaagcgcgaa atcgctggta tccgcacgcc
    16081 gcatcggcat gacttggatg gcgttgtgcc ttgcgggcgc ggtggcggtc ggttatttcg
    16141 gcattgcgta tttcggtgca aatcccgaca aagtttcttc tatgagcggc aaccacgaac
    16201 gcatcttcat cgcgctttcc accctgctgt tcaacccttg gatcgccggc atcattttga
    16261 gcgcgatttt ggcggcggtg atgtccaccc tgtcctgcca gcttttggtt tgctccagcg
    16321 cgattaccga agacttctac aaaggctttt tgcgtaaaaa cgcgcaacag tcggaactgg
    16381 tatgggtcgg ccgcctgatg gtcttggcga ttgccgtgat ttccatcctg attgcttccg
    16441 atccgaacag caaagtattg ggactggtgt cttatgcgtg ggcaggtttc ggtgcggcgt
    16501 tcggcccgat tgtgattctg tccgtattgt ggaaacgcat cactgcctac ggcgcgttgt
    16561 cgggtatggt tgcaggtgcc ttaaccgtag tcgtatgggc ggaatggata aaaaaaccgg
    16621 ctcaagcagc aggcgaaagc ggcttgttga cgatgtacga aatcgtcccg ggctttattg
    16681 tttgcttaat cattgccgtg ttggtttccc tgttcaacaa agaaccttcg cgcgaaatcc
    16741 aagaacgctt tgaaaaagcc gacgcagatt accgcgccgc ccgataaacc cctttcagac
    16801 ggcacggcat cccggttcgt gccgtctgaa cccgtttccg ggtcaaacac aaataaacct
    16861 tgcctgcata caggccttat ttgtgtttta cccgtttcat tttcaaagaa acggaagcac
    16921 ctaccctgcc cgcatttgaa gccttgtatt tcatctgcgg atttattacg atattaccgt
    16981 attcaggccg caccgatgcc gcctgccccc cgaaaaactt tggagaatcc aaaaaatgtt
    17041 tcattttgca tttccggcac aaactgccct gcgccaagcg ataaccgatg cctaccgccg
    17101 taatgaaatc gaagccgtac aggatatgtt gcaacgtgca cagatgagcg acgaagagcg
    17161 caacgccgcc tccgagcttg cccgccgttt ggttacccaa gtccgcgccg gccgcaccaa
    17221 agccggcggc gtggatgcgc tgatgcacga gttttcactc tccagcgaag aaggcatcgc
    17281 gctgatgtgt ctggcagaag ccctgctgcg tatccccgac aacgccacgc gcgaccgcct
    17341 gattgccgac aagatttcag acggcaactg gaaaagccat ttgaacaaca gcccttccct
    17401 cttcgtcaac gccgccgcct ggggcctgct gattaccggc aaactgaccg ccacaaacga
    17461 caaacaaatg agttccgcac tcagccgcct gatcagcaaa ggcggtgccc ccctcatccg
    17521 tcaaggtgtg aactacgcaa tgcgcctgtt gggcaagcag tttgtgaccg gacagaccat
    17581 tgaagaagcc ctgcaaaacg gtaaagaacg cgaaaaaatg ggctaccgct tctccttcga
    17641 tatgttgggc gaagccgcct acacccaagc cgatgccgac cgctactacc gcgactatgt
    17701 cgaagccatc cacgccatcg gcaaagatgc ggcaggacaa ggcgtttacg aaggcaacgg
    17761 catttccgtc aaactttccg ccatccatcc gcgctactcg cgcgcccaac acggccgtgt
    17821 gatgggcgaa ctgttgccgc gcctgaaaga gctgttcctt ttgggtaaaa aatacgatat
    17881 cggtatcaac atcgatgccg aagaagccaa ccgtctggag ctgtctttgg atttgatgga
    17941 ggctttggtt tcagaccctg acttggctgg ctacaaaggt atcggtttcg ttgtccaagc
    18001 ctaccaaaaa cgttgtccgt tcgttatcga ctacctgatc gaccttgccc gccgcaacaa
    18061 ccaaaaacta atgatccgcc tcgtcaaagg cgcgtattgg gacagcgaaa tcaaatgggc
    18121 gcaagtggac ggcttgaacg gctatccgac ctacacccgc aaagtccaca ccgacatctc
    18181 ctacctcgcc tgcgcgcgca aactgctttc cgcgcaagac gcggtattcc cgcaatttgc
    18241 cacccacaac gcctacactt tgggcgcaat ctaccaaatg ggtaaaggca aagattttga
    18301 acaccaatgc ctgcacggta tgggcgaaac cctgtacgac caagtcgtcg gcccgcaaaa
    18361 cttaggccgc cgcgtgcgcg tgtacgcccc agtcggcaca cacgaaaccc tgctcgccta
    18421 cttggtgcgc cgcctgttgg aaaacggcgc gaactcgtct ttcgtcaacc aaatcgttga
    18481 tgaaaacatc agcatcgaca cgctcatccg cagcccgttt gacaccatcg ccgaacaagg
    18541 catccacctg cacaacgccc tgccactgcc gcgcgatttg tacggcaaat gccgtctgaa
    18601 ctcgcaaggc gtggacttga gcaacgaaaa cgtattgcag cagcttcaag aacagatgaa
    18661 caaagccgcc gcgcaagact tccacgccgc atccatcgtc aacggcaaag cccgcgatgt
    18721 cggcgaagca caaccgatta aaaatcctgc cgaccacgac gacatcgtcg gcacagtcag
    18781 ctttgccgat gccgcgcttg cccaagaagc ggttggcgca gccgttgccg cgttccccga
    18841 atggagtgcg acacctgccg ccgaacgcgc cgcctgcctg cgccgttttg ccgatttgct
    18901 ggagcagcac accccagcac tgatgatgct tgccgtgcgc gaagcaggca aaacgctgaa
    18961 caacgccatt gccgaagtgc gcgaagccgt cgatttctgc cgctactacg caaacgaagc
    19021 cgaacatacc ctgcctcaag acgcaaaagc cgtcggcgcg attgtcgcca tcagcccgtg
    19081 gaacttcccg ctcgccatct ttaccggcga agtcgtttcc gcattggcgg caggcaacac
    19141 cgtcatcgcc aaacccgccg aacaaaccag cctgattgcc ggttatgccg tttccctcat
    19201 gcacgaagcc ggcatcccga cttccgccct gcaactcgtc ctcggcgcag gcgacgtggg
    19261 tgcggcattg accaacgatg cccgcatcgg cggcgtgatt ttcaccggct cgaccgaagt
    19321 ggcgcgcctg atcaacaaag cccttgccaa acgcggcgac aatcccgtcc taattgccga
    19381 aaccggcgga caaaacgcca tgattgtcga ttccactgca cttgccgaac aagtctgcgc
    19441 cgacgtattg aactccgcct tcgacagcgc gggacaacgc tgctccgccc tgcgtatctt
    19501 gtgcgtccaa gaagacgttg ccgaccgtat gctcgacatg atcaaaggcg cgatggacga
    19561 actcgtcgtc ggcaaaccga ttcaactcac caccgatgtc ggctccgtca tcgatgccga
    19621 agcgcagcaa aacctgttga accacatcaa caaaatgaaa ggcgttgcca agtcttacca
    19681 cgaagtcaaa accgctgccg atgtcgattc cgaaaaatcc acgttcgtcc gccccatcct
    19741 gtttgaattg aacaacctca acgaactgca acgcgaagtc ttcggtcccg tcctgcacgt
    19801 cgtccgctac cgcgccgacg aactcgacag cgtcatcgac caaatcaaca gcaaaggcta
    19861 cgccctgacc cacggcgtac acagccgcat cgaaggcacg gtacgccaca tccgcagccg
    19921 catcgaagcc ggcaacgttt acgtcaaccg caacatcgtc ggcgcagtcg tcggcgtaca
    19981 gcccttcggc ggacacggtc tgtccggcac aggtcccaaa gcaggcggtt cattctacct
    20041 gcaaaaactg acccgcgccg gcgaatgggt tgccccgacc ctgagccaaa tcggacaggc
    20101 ggacgaagcc gcactcaaac gcctcgaagc actggttcac aaactaccgt tcaacgccga
    20161 agagaaaaaa gccgcagcgg ccgctttggg acacgcccgc atccgcaccc tgcgccgtgc
    20221 cgaaaccgtc cttaccggac cgaccggcga gcgcaacagc atctcatggc acgcgcccaa
    20281 acgcgtttgg atacacggcg gcagcctcct gcaagccttc tgcgccctaa ccgaacttgc
    20341 cgcctccggc gtacaggcag tggtcgaacc cgacagcccg ctggcttcct acaccgccga
    20401 cttggaaggt ctgctgctgg tcaacggcaa acccgaaacc gccgacatca gccacgttgc
    20461 cgccctgtcg cctttggaca gcgcgcgcaa acaggaactt gccgcccacg acggcgcact
    20521 catccgcatc ctcccttcgg aaaacggact cgacatcctg caagtgtttg aagaaatctc
    20581 ttgcagcgtc aacaccacag ccgccggcgg caacgccagc ctgatggcgg ttgccgactg
    20641 attttaccga aatccccgag cgcggcccgt gaaccaatgc cgtctgaaaa cctttcagac
    20701 ggcattttta ta
//
LOCUS       DAWKQB010000021        20566 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.021, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000021 DAWKQB010000000
VERSION     DAWKQB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20566
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.021"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(133..468)
                     /locus_tag="WJL14_000587"
     CDS             complement(133..468)
                     /locus_tag="WJL14_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7655698.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            597..1502
                     /gene="mtrA"
                     /locus_tag="WJL14_000588"
     CDS             597..1502
                     /gene="mtrA"
                     /locus_tag="WJL14_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ7655699.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1564..2052)
                     /locus_tag="WJL14_000589"
     CDS             complement(1564..2052)
                     /locus_tag="WJL14_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7655700.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2199..3101
                     /locus_tag="WJL14_000590"
     CDS             2199..3101
                     /locus_tag="WJL14_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7655701.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLAEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3142..4272)
                     /locus_tag="WJL14_000591"
     CDS             complement(3142..4272)
                     /locus_tag="WJL14_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7655702.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4477..7101
                     /gene="alaS"
                     /locus_tag="WJL14_000592"
     CDS             4477..7101
                     /gene="alaS"
                     /locus_tag="WJL14_000592"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ7655703.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSSENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGGHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7163..7885
                     /locus_tag="WJL14_000593"
     CDS             7163..7885
                     /locus_tag="WJL14_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655704.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHV"
     gene            8603..9862
                     /locus_tag="WJL14_000594"
     CDS             8603..9862
                     /locus_tag="WJL14_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ7655705.1"
                     /translation="MEELPEYITRGVFDFMFGIEAKVRQIQEEANIQAAATIGRLHDA
                     LKEEGIYEEHELRLFITRLLFLFFADDSAVFRRNYLFQDFLENCKEADTLGDKLNQLF
                     EFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTAKQHRALIDCGNFDWRNIS
                     PEIFGTLFQSVMDAQERREAGAHYTEAANIDKVINGLFLENLRAEFEAVKALKRDKAK
                     KLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAEALKDKADGLFDSPSVQ
                     CRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEVACHTLPLEDAAEIIHA
                     NSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKGYGLLDYVCNWYVKAAG
                     IMAQHPQVQTAFVSTNSICQGQQVEIL"
     gene            9986..11014
                     /locus_tag="WJL14_000595"
     CDS             9986..11014
                     /locus_tag="WJL14_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEZ7655706.1"
                     /translation="MPSEKTLYDYPDIKGEPEKHAVANINPYLIDAPDLIIAKRSRPI
                     HCEPDMVNGSKPTEGGNLILSTAEKDALIAAEPLAEQYIRPFIGADEFLNGKTRWCLW
                     FHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATPWLFQKIRQPSDGN
                     YLIIPSVSSESRRFIPIGYLSFETVVSNLAFILPNATLYHFGILSSTMHNAFMRTVAG
                     RLKSDYRYSNTVVYNNFPFPESCRLPSENDRPDPLRAAVEAAAQTVLDARGQYRREAQ
                     EAGLPEPTLAELYAPDAGYTALDKAHATLDKAVDKAYGYKTGKNTDDEAERVAFLFEL
                     YRKAAAIA"
     gene            complement(11816..12499)
                     /locus_tag="WJL14_000596"
     CDS             complement(11816..12499)
                     /locus_tag="WJL14_000596"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ7655707.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            12795..15098
                     /gene="parC"
                     /locus_tag="WJL14_000597"
     CDS             12795..15098
                     /gene="parC"
                     /locus_tag="WJL14_000597"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ7655708.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIVKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTI
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            15118..16635
                     /locus_tag="WJL14_000598"
     CDS             15118..16635
                     /locus_tag="WJL14_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7655709.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGH
                     ENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            16687..17154
                     /locus_tag="WJL14_000599"
     CDS             16687..17154
                     /locus_tag="WJL14_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7655710.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            17258..18010
                     /locus_tag="WJL14_000600"
     CDS             17258..18010
                     /locus_tag="WJL14_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7655711.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            18833..18945
                     /locus_tag="WJL14_000601"
                     /pseudo
     CDS             18833..18945
                     /locus_tag="WJL14_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(19044..19250)
                     /locus_tag="WJL14_000602"
     CDS             complement(19044..19250)
                     /locus_tag="WJL14_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ7655712.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(19345..20514)
                     /gene="metZ"
                     /locus_tag="WJL14_000603"
     CDS             complement(19345..20514)
                     /gene="metZ"
                     /locus_tag="WJL14_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ7655713.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
BASE COUNT         5134 a         5853 c         5006 g         4573 t
ORIGIN      
        1 tcacgcttac cccttgtttc tcaaatgccg tctgaaataa gcggcttaat atattgttta
       61 cagtattggg aagcataaca gacaaaatgc cgtctgaaat attttcagac ggcatttctt
      121 atccgaaacg gattattttt gcgtttcaac cgcttccaat gcacgcaggg cgtaagtgta
      181 agcggcaccg gcattcaggg cgatggcggt tgccaatgcg cctgcgattt cgctgtcggt
      241 cgcaccggct ttggcggcag cggcggcgtg aacgctgatg cagctctcgc aacgagtggt
      301 aatggcaacg gcgatggcaa tcagttcgcg cgttttggca tcaagtgctt cggcggcggc
      361 ggcctgttcc aatgcgccgt aggcctgcag cattttagga tgcgccttac ccagctcgcc
      421 gaacgatttt ttaaccaatg cggtatgttc tttccaatct ttaaacattt tcttttcctt
      481 tctcttgcgt ttaaccctga tacgcgcttg cgtatctgtt ttcgatgtgc gtattattgc
      541 aattattcag ttgtgtttct cgtttaatca tctcatttta tggttcaaaa agatttatgg
      601 acattctgga caaactggtc gatttcgccc aattgacggg cagtgtggat gtgcagtgcc
      661 ttttgggcgg acaatggtcg gtacggcatg aaaccttgca acgcgaagga ttggtacaca
      721 ttgttacatc gggcagcggc tatctctgca tcgacggcga aacttccccg cgtccggtca
      781 gtacagggga tattgtattt ttcccgcgcg gcttgggtca tgtgttgagc cacgacggaa
      841 aatgcggaga aagtttacaa ccggatatgc ggcagcacgg tgcgtttacg gtcaagcagt
      901 gcggcaacgg acaggatatg agcctgtttt gcgcccgttt ccgctacgac acccacgccg
      961 atttgatgaa cgggctgcct gaaaccgttt ttctgaacat tgcccatccg agtttacagt
     1021 atgtggtttc aatgctgcaa ctggaaagca aaaaaccttt gacggggacg gtttccatgg
     1081 tcaacgcatt gtcgtccgtc ctgctggtgc ttatcctgcg cgcctatctc gaacaggata
     1141 aggatgtcga actctcgggc gtattgaaag gttggcagga caaacgtttg ggacatttaa
     1201 tccaaaaggt gatagacaaa ccggaagacg aatggaatgt cgacaaaatg gtggcggctg
     1261 ccaatatgtc gcgcgcgcaa ctgatgcgcc gtttcaaaag ccgggtcgga ctcagcccgc
     1321 acgcctttgt gaaccatatc cgcctgcaaa aaggcgcgtt gctgctgaaa aaaaacccgg
     1381 attcggtttt gtcggtcgca ctgtcggtag gctttcagtc ggaaacgcac ttcggcaagg
     1441 cgttcaaacg gcaatatcac gtttcgccgg gtcaataccg gaaagaaggc gggcaaaaat
     1501 aaatcggggc ttcaaacgca aatgccgtct gaaaaggctt tcagacggca tttgcgtacc
     1561 gcgtcatttc aagggctgca tcttcatcac ttccatcaaa aagttggtaa atgcggggtt
     1621 gttgggtttg acatccatat ttttccaacg ctgctgccag ccgcgcaagg cattctggat
     1681 atacagcttg gactgttccg tattgattgc gccccgctgg ctgtctatcg ccgaacgcag
     1741 gtagatttca tacatactgt catcgacggc attgcgtccg accaggcgtt ttctgaagtt
     1801 gttcagatat tgcgccgcct gaaccttggt cattttaccg atacccacct gatagcccaa
     1861 gcgcgtcgct tcatcgctga ttttggcaac atccgtccaa tgcgaagagg caaggcggaa
     1921 accttttgca ggtgcttccg ttttgacggt attgatagga ttcacgggga tttccgtcaa
     1981 tgtgggcaca taaatagact ggcagccgga aagaactgcc acaatggaaa gagggataag
     2041 gtattttttc atgcccccat tataatcaag tttgccttga gaaaacaaat tgttcggcaa
     2101 gaaaaataaa atttcggcat cagaagcagg caaaaacaca ttccacaagc cttgccgcaa
     2161 ggtttacaat ccgaccgtcc ttatcgcaac gaccgtttat ggataccgca aaaaaagaca
     2221 ttttaggatc gggctggatg ctggtggcgg cggcctgctt taccattatg aacgtattga
     2281 ttaaagaggc atcggcaaaa tttgccctcg gcagcggcga attggtcttt tggcgcatgc
     2341 tgttttcaac cgttgcgctc ggggctgccg ccgtattgcg tcgggacacc ttccgcacgc
     2401 cccattggaa aaaccactta aaccgcagta tggtcgggac gggggcgatg ctgctgctgt
     2461 tttacgcggt aacgcatctg cctttggcca ccggcgttac cctgagttac acctcgtcga
     2521 tttttttggc ggtattttcc ttcctgattt tgaaagaacg gatttccgtt tacacgcagg
     2581 cggtgctgct ccttggtttt gccggcgtgg tattgctgct taatccctcg ttccgcagcg
     2641 gtcaggaaac ggcggcactc gccgggctgg cgggcggcgc gatgtccggc tgggcgtatt
     2701 tgaaagtgcg cgaactgtct ttggcgggcg aacccggctg gcgcgtcgtg ttttaccttt
     2761 ccgtgacagg tgtggcgatg tcatcggttt gggcgacgct gaccggctgg cacaccctgt
     2821 cctttccatc ggcagtttat ctgtcgtgca tcggcgtgtc cgcgctgatt gcccaactgt
     2881 cgatgacgcg cgcctacaaa gtcggcgaca aattcacggt tgcctcgctt tcctatatga
     2941 ccgtcgtttt ttccgctctg tctgccgcat tttttctggc cgaagagctt ttctggcagg
     3001 aaatactcgg tatgtgcatc atcatcctca gcggtatttt gagcagcatc cgccccactg
     3061 ccttcaaaca gcggctgcaa tccctgttcc gccaaagata aaaaatgccg tccgaacatc
     3121 cttcagacgg catatcgggc tttatttccc cgccttcaca tcctgccact ggcgcaccat
     3181 aaacttcaat gccgccggct ggataggcac catgataaag ctgtttttca aatcctcctc
     3241 ggttgggaaa atcgtattgt cgtttttaaa ttcgtcttcc atcagctcac gcgcaggctt
     3301 gctcgaaggc gcgtaagtaa cgaaattgcc gtttttcgcc gacacttccg ggtcgaggaa
     3361 gtcgttgatg tatttgtgcg cgttggcgac gtttttcgca tctttcggaa tcacgaaaga
     3421 atccacccaa atccccacgc cctctttggg catcatcacg cggatttttt ccttgccgcc
     3481 cgcttcttcg gcacggcgtt tggcgatgtt caaatcgccg ccgaaaccga ttgttacgca
     3541 ggtatcgccg cgcgccaaat catcgataaa gccggacgaa gtaaagcgtt tgatattggg
     3601 gcggtttttc ttgagtaggg cggttgcctc cctgatgtct tccgtattgc tgctgttcgg
     3661 gtttttaccc aaatagttca acaccatagg atagatttcc gccgcgctgt ccaaatagct
     3721 gatgccgcat tgcttgagtt tggacgtgta ttcggggtcg aacaccaaat cccactggtt
     3781 gtccggcagc ttgtccgtac ccaaagcctt tttcacgcgt tcggtattga tggcgaaggt
     3841 atttgtcccc caataaaacg gcacggcgta ttcgtggccg ggatcgaccc cgtccatcag
     3901 cctcatcatt tcggggttga ggtgtttata attgggaatc agcgacttat cgattttctg
     3961 atacgcacct gccttaatct gcctgcccac aaacgcattg gacggcgcga caatgtcgta
     4021 accagatttt ccggtcagca ccttgctttc cagcgtttca tcgctgtcgt acacatcata
     4081 agtaaccttg atgccgtttt tcttttcaaa atcggcaacg gtttccggat cgacgtattc
     4141 cgaccagttg taaattttca atacgtttcg gttttccgcc ggcgccggtt tttcggcagg
     4201 cggtttgtcc gaaccgccgc acgctgcaag cagcaaagca gtcaggacgg ccaggggcag
     4261 atgtttggtc attatcattc cttgcatatc gggttggaga aagcggccat tatagccgat
     4321 attggcaaca gggcttcaga cggcattcaa aatcccgcca cactcttccg aaaaccgccg
     4381 cttccatagc tagaaacagg gatttgcggt aagataccgc cgttcgtttt ccctgctttt
     4441 accatgacaa gacatttgag agacattgaa aaaattatga aaacctccga actgcgccaa
     4501 aaattcctaa aattttttga aaccaaaggc cacaccgtcg tccgctcttc cagcctcgtg
     4561 ccgcacgacg acccgaccct gctgtttacc aacgcgggca tgaaccagtt taaagacgta
     4621 ttcttaggtt tcgacaaacg cccgtacagc cgcgccacca ccgcgcaaaa atgcgtacgc
     4681 gcaggcggca agcacaacga cttggaaaac gtcggctaca ccgcccgcca ccacaccttc
     4741 tttgaaatga tgggcaactt ctccttcggc gactacttca aacgcgacgc catccacttc
     4801 gcttgggaat ttctgacttc ccccgaatgg ctcaacatcc ctaaagacaa actgttggcg
     4861 accgtttacg cggaagacga cgaagcctac aacatctggt tgaacgaaat cggtatgccg
     4921 tccgagcgca tcgtccgcat cggcgacaac aaaggcgcga aatacgtgtc cgacaacttc
     4981 tggcaaatgg gcgacaccgg cccttgcggc ccctgctccg aaattttcta cgaccacggc
     5041 gaagaaatct ggggcggcat tcccggcagc cccgaagaag acggcgaccg ctggatcgaa
     5101 atttggaact gcgtatttat gcagttcaac cgcgacgaac aaggcaatat gaatccgctg
     5161 cccaagccgt ccgtcgatac cggtatgggc ttggaacgta tggccgccgt catgcagcat
     5221 gttcacagca actacgaaat cgacttgttc caagacctgc tcaaagccgt tgcccgcgaa
     5281 accggcgcag ctttcagcat ggatgaaccc agcctgaaag tcatcgccga ccacatccgc
     5341 tcctgctcct tcctgattgc agacggcgta ttgccgtcca acgaaggccg cggctacgta
     5401 ttgcgccgca ttatccgccg cgccgtgcgc cacggttaca aactgggtca aagcaaaccg
     5461 ttcttccaca aactggtggc cgatttggtc aaagagatgg gcggtgccta ccctgaattg
     5521 aaagaaaaac aagcacaaat tgaagaagca ttgaaaaacg aagaaagccg ttttgcccaa
     5581 acgctggaaa ccggtatggc tttgttggaa aacgcgctgg ccaaaggcgg caaaacactc
     5641 gacggcgaaa tcatcttcaa actctacgac acctacggct tcccatacga cttgactgcc
     5701 gacatctgcc gcgaacgcaa tatcgaactg gacgaagcag gcttcgagcg cgaaatggaa
     5761 gcccaacgcg cacgcgcacg cgccgcccaa agcttcaaag ccaacgccca actgccttat
     5821 gacggtcaag acaccgagtt taaaggttat agcgaacgcc aaaccgaatc caaagtcctc
     5881 gccctctaca aagacggcga gcaagtcaac gaattgaacg aaggcgacag cggcgcagtc
     5941 gtcatcgact ttaccccgtt ctatgcagaa tccggcggcc aagtcggcga tgtcggctat
     6001 atcttctcaa gcgaaaaccg ctttgaagta cgcgataccc aaaaaatcaa agcggccgtg
     6061 ttcggacaat tcggcgtaca aacttcaggc cgtctgaaag tcggcgacag cgttaccgcc
     6121 aaagtggacg acgaaatccg caatgccaat atgcgcaacc acagcgcaac ccacttgatg
     6181 cacaaagccc tgcgcgacgt attgggcgga cacgtcgaac aaaaaggctc tttagttacc
     6241 gccgaatcca cccgtttcga catttcccat ccccaagcgg tcactgccga agaaatcgcc
     6301 gaagtcgaac gccgcgtcaa cgaagccgtt ttggcgaacg ttgccgtcaa tgcagccatt
     6361 atgagcatgg aagacgcgca aaaaaccggc gcgatgatgc tcttcggcga aaaatacggc
     6421 gaagaagtgc gcgtactgca aatgggcggt ttctctaccg aattgtgcgg cggcacacac
     6481 gtttcacgca ccggcgacat cggcctcttc aaaatcatca gcgaaggcgg tattgccgca
     6541 ggcgtgcgcc gtatcgaagc catcaccggc ctgaacgcac tcaaatgggc gcaagagcaa
     6601 gagcgtttgg tgaaagacat tattgccgaa accaaagccc aaaccgaaaa agacgtactg
     6661 gcgaaaatcc aagcaggcgc ggcacacgcc aaagcattgg aaaaagaatt ggcacgcgcc
     6721 aaagccgaac tcgccgtcca cgcaggcgca aaactcttgg acgacgcaaa agacttgggc
     6781 gcagccaaac tcgttgccgc ccaaatcgaa gccgacgcag ccgccctgcg cgaaatcgta
     6841 accgacctga ccggaaaatc cgacaacgcc gtgattcttt tagcggcagt aaacgacggc
     6901 aaagtctccc tgtgcgccgg cgtatccaaa ccgttgaccg gcaaagtgaa agcaggcgat
     6961 ctggttaaat ttgcagccga acaagtcggc ggcaaaggag gcggccgtcc cgacttggcg
     7021 caggccggcg gcacggatgc cggcaaattg cccgaaatgc tggtaagcgt ggaaagctgg
     7081 ctttgtcaga aattaagtta aatttaaatc ttaataagcc atttgagtct tgttcaaatg
     7141 gcttatattt tggattgtta gaatgaatac taaaacagag ttacaaaaac ttttagaaga
     7201 ggatatatca actttaacag aaacactaat ttgtgcagat gctcttcctc caagatatgt
     7261 acgtagcatt gcaactccta ttgtccgtcg atggttaatt gacaaacaat taaatattct
     7321 cgcaaaagaa ataggtttaa ctatagaatt gcccatcctt gatacaagtt tggtatttga
     7381 aaaattgtca accttagaga ataaagttaa tttttatatg gctggtggag tttatttagg
     7441 cggagaattt attagctcta tatatcattc atcacaagaa ttttctggtg aaccaataat
     7501 ctatgctgaa ccaaatataa ttttatgtcc tgctgaaaaa tttttaactt taaaacgagt
     7561 tttccataat ggaaatattt ttaatatgaa tcagataatt acttttttat ccaataaaca
     7621 aggaggagta cattttgata aaaattacga taaatataaa acttggcaag ttgctataga
     7681 aaaagcagca aattttttaa aactcggtaa tccatataat gaagataaat tatctcttag
     7741 tgaagaacat gatactattt tagtagtttt accattagaa aaaggatatg aatggaattg
     7801 tcttgagata gaagtactat ccgctgctca atctctagca aatatctatt gcaacaaagt
     7861 tcgcctcata gatggtcatg tttagaagga ataaaagaaa aagcatagtt ggtaattttt
     7921 agttacgtag ggtgggcttc agcccaccgt tttttcattt ttgattgttg attggtgggc
     7981 taaagcccac cctacggtta ctaaaatccc gtcattacct cgcaggtggg aatccggttt
     8041 gttcggtttc ggttttttag ggacagtgcc gcaacattaa atttccagat tcccactttc
     8101 gcacaaataa cagcgcaaat agggcaaaga taaggatatg ggtttttttc tttgaattta
     8161 ctatacaatt cgcccgtccc cgttatagaa acccaagccg atgaaaaccc tgctccaact
     8221 ccaaaccgcc gcacaaaact tcgccgccta ctacaaaaac caaaccgacg aacgccgcga
     8281 gaaagacacc ttctagaacg aatttttcgc cattttcggc atcgaccgca aaaacgtcgc
     8341 ccacttcgaa taccccgtca aagaccctgc cgacaacacc caattcgtcg atatattttg
     8401 ggaaggcatc ttccttgccg aacacaaatc cgccaacaaa aacctgacca aggccaaaga
     8461 gcaggcggaa cgttatttac aggaaatcgg gcgcaccaag ccctccgcgc tgcccgaata
     8521 ttacgccgtc agcgattttg cccatttcca cctttaccgc cgcgtacctg aagaaggcgc
     8581 agaaaaccaa tggcagttcc ctttggaaga attgcctgaa tacatcacgc gcggcgtttt
     8641 cgacttcatg ttcggcatcg aagccaaagt ccgccaaatt caagaagaag ccaacattca
     8701 agcggcggcg accatcggca ggctgcacga cgcgctcaaa gaagaaggca tttacgaaga
     8761 acacgagctg cgcctcttca tcacgcgcct gcttttcctc ttttttgccg acgacagcgc
     8821 cgttttccgg cgcaactacc ttttccaaga ctttttagaa aactgcaaag aagccgacac
     8881 gctcggcgac aagctcaatc aactctttga atttctcaac acacccgacc aaaagcgcag
     8941 caagacccaa agcgaaaaat ttaaaggttt cgaatacgtc aacggcggtc ttttcaaaga
     9001 acgcctgcgc actttcgact tcactgccaa gcagcaccgc gccttaatcg actgcggcaa
     9061 tttcgactgg cgcaacatca gtccagaaat cttcggcacg ctcttccaat ccgtcatgga
     9121 cgcgcaagag cggcgcgaag cgggcgcgca ctacaccgaa gccgccaata tcgacaaagt
     9181 catcaacggc ctttttttag aaaacctgcg tgccgaattt gaagccgtca aagccctcaa
     9241 acgcgacaaa gccaaaaaac tcgccgcctt ctaccaaaaa atccaaaacc tgcaattcct
     9301 cgaccctgcc tgcggctgcg gcaacttcct tatcgtcgcc tacgaccgca tccgcgccct
     9361 tgaagacgac atcatcgccg aagccctcaa agacaaagca gacggcctgt tcgacagccc
     9421 gtccgtccaa tgccgtctga aacagtttca cggcatcgaa atagacgaat ttgccgtcct
     9481 catcgcccgc accgccatgt ggctcaaaaa ccaccaatgc aacatccgca cacaaatccg
     9541 cttcgacggc gaagtcgcct gccatacgct gccgctcgaa gacgccgccg aaatcatcca
     9601 cgccaacagc ctccgcacac cttggcaggc ggcggactac atcttcggca atcccccctt
     9661 tatcggctcg acctaccaaa ccaaagagca gaaaaacgac ctcgaaagca tctgcggcca
     9721 tatcaaaggc tacggcctgt tggattacgt ctgcaactgg tacgtcaaag ccgcaggcat
     9781 catggcgcag catccccaag ttcagacggc atttgtttcc accaattcca tctgccaagg
     9841 ccagcaggtc gaaatcctct agggcagcct tttaaaccaa ggcatcgaaa tccactttgc
     9901 ccaccgcacc ttccaatgga cgagccaagc cgcaggcaaa gccgccgtcc actgcatcat
     9961 cgtcggcttc cgccaaaagc cgccaatgcc gtctgaaaaa accctctacg actatcccga
    10021 catcaaaggc gaacccgaaa aacacgccgt agccaacatc aatccttatc tgatcgatgc
    10081 gcccgatttg attatcgcca agcgcagccg tcccatacat tgcgaacctg atatggtcaa
    10141 cggaagcaaa ccgaccgaag gcggcaacct tatcctttca accgccgaaa aagatgccct
    10201 gattgccgcc gaacccttgg cggagcaata catccgcccc tttatcggcg cggatgagtt
    10261 tctcaacggc aaaacccgtt ggtgcctgtg gtttcacggc gtatccgatg tcaaacgcaa
    10321 ccacgacctg aaacaaatgc cccaagttca agcccgtatt caggcggtca aaaccatgcg
    10381 cgaagccagc agcgacaaac aaactcaaaa agatgcagca accccgtggc tttttcaaaa
    10441 aatccgccag ccttcagacg gcaattatct gattattccg agcgtgtcgt ctgaaagccg
    10501 ccgtttcatc cccatcggtt atctgtcgtt tgaaacagtt gtcagcaatc tggcatttat
    10561 ccttccaaac gccaccctct accacttcgg catcctcagc tccaccatgc acaacgcctt
    10621 tatgcgtacc gttgcaggtc gtctgaaaag cgattatcgc tactctaata ccgtcgtgta
    10681 caacaacttc cccttccccg aaagctgccg gttgccgtct gaaaacgacc gccccgaccc
    10741 gctccgcgcc gccgtcgaag ccgccgccca aaccgtcctc gacgcgcgcg gacaataccg
    10801 ccgagaagcg caggaagccg gtttgcccga gccgaccctc gccgaactct atgcgcccga
    10861 cgcaggctat accgccctcg acaaagccca cgccaccctc gacaaggcag tcgataaagc
    10921 ctacggctac aaaacaggca aaaataccga cgacgaggca gaacgcgtcg ccttcctgtt
    10981 cgagctgtac cgcaaggcgg cggcaattgc gtagtagttg cgtagtagtt gcgtaggata
    11041 gttgcgtagg gtgggcttca gcccaccaac tcccaccaac tcccaccatc ttcaccctaa
    11101 aaccccgtca tctccgcaaa agcaattgcg tagggtgggc ttcagcccac cgtttttctt
    11161 cattttcggt tgttgattgg caggctgaag cccaccctgc ggttacaaaa atcccgtcat
    11221 tcccgcgcag gcgggaatcc agacctttcg gggcggaaac ttatcggata aaacgatttc
    11281 ccctgcatcc tggattccca ctttcatggg aatgacggga tataggtttc cctacggacg
    11341 tgttcagatt cccgccttcg cgggaatgac ggcggagaag tttctgtctt tccgataact
    11401 taccgtaact taaaatcccg tcattaccgc aaaagcagtt gctacggtta caaaaatccc
    11461 gtcatcccca cgaaagcgaa aatcctgcct gtcggatttc ggttttttcg ggcgtttcgg
    11521 gaacttataa atcgtcattc ccgcgcaggc gggaatccag aacgcgggac ggcggcaata
    11581 ttcaaaggtt gtctgaaaat tcagaggttc tagattcccg cctgcgcggg aatgacggcg
    11641 gagcggtttc tgcttttccg ataaatgacc gcaacctaaa acccatcctt cccacgaaaa
    11701 cagaaaaaca aaaacctaaa atcccgtcgt tctcgcgaaa gcagttgcta cgtttaccgt
    11761 caaaaatgcc gtctgaacgc tcaagccttc agacggcatt ttttatttca cacccttaca
    11821 ggtagaattt ttcgatgact ttcaaattgt cgtccaattt gtacaccaac ggctgaccgg
    11881 tcgggatttc caagcccatg atgtcttcgt cggaaatgcc ctcgatgtgt tttgccagcg
    11941 cgcgcaggga gttgccgtgc gccgccacca agacgcgttt gccgctcaaa atcgcggggg
    12001 cgatttggtc ttcccaaaac ggcaatacgc gctccagcgt tacttgcagg ttttcgccgt
    12061 cggggataac atcggcaggc agatgggcat agcggcggtc tttgtgtgcg gaaaactcat
    12121 cgtctttgtc caaaagcggc ggcagggtgt cgtagctgcg ccgccagatg cggacttgct
    12181 cgtcgccgta ttgttcggcg gtttgttttt tgtccaggcc ttgcagtcgg ccgtagtggc
    12241 gttcgttcag ccgccatgtt ttgatttgcg gtacgaacag ttggtcggat tcttccaaaa
    12301 cgatgttgca ggtcttaatc gcgcgggtca ggacggatgt gaaggcgatg tcgaactcat
    12361 agccgttttc tttcagtttt ttaccggcgg cagcagcctc ggcaagcccc tgctcgctca
    12421 gcttcacgtc gcgccagcct gtaaacaggt ttttcgcgtt ccattcgctt tgtccgtggc
    12481 ggataaatac caattccata tcgtctccaa tgtgtgaaag tgggaaagcc ttatttataa
    12541 catattttca cattcccgta tttgattcag attcagacac gcgcccacta tggtttgccg
    12601 ttttgattta caataatgcc ctttgcttta cattccgcat acacaatgtt tcaacacaca
    12661 ggacgacaca taaagcaccg ccctatgtgt tgccctgatt tggaaggggt tgccccctcc
    12721 caaataaagt ctgattctac cgccccaaag gacagatgtc caagtggcgg gggttcaacc
    12781 gaaaaggaaa tacgataaat atgcaaccgc acgctccaca taccgattcc aatacgctga
    12841 tgctcggccg atacgccgaa cgcgcctatc tcgaatacgc catgagcgtg gtcaaaggcc
    12901 gcgcgctgcc tgaagtttca gacggccaaa agcccgttca gcggcgcatt ttgtttgcca
    12961 tgcgcgatat gggtttgacg gcgggggcga agccggtgaa atcggcgcgc gtggtcggcg
    13021 agattttggg taaataccac ccgcacggcg acagttccgc ctatgaggcg atggtgcgga
    13081 tggcgcagga ttttaccttg cgctatccct taatcgacgg catcggcaac ttcggttcgc
    13141 gcgacggcga cggtgcggcg gcgatgcgtt acaccgaagc tcggctgacg ccgattgcgg
    13201 aattgctgtt gtccgaaatc aatcagggaa cggtggattt cgtgccgaac tacgacggcg
    13261 cgtttgacga gccgctgcac ctgcccgccc gcctgcctat ggtgttgctc aacggcgcgt
    13321 caggcatcgc ggtgggcatg gcgaccgaga ttccgtcgca caatttgaac gaagtgacgc
    13381 aggcagcgat tgcgctgttg aagaagccga cgctggaaac cgccgacctg atgcaatata
    13441 ttcctgcgcc cgattttgcc ggcggcggtc aaatcatcac gccggcggac gaattgcgcc
    13501 ggatttacga aaccggcaaa ggcagcgtgc gcgtgcgtgc gcgttatgaa atcgagaagc
    13561 tggcgcgcgg acagtggcgc gtcatcgtaa ccgagctgcc gccgaacgcc aattccgcca
    13621 aaatccttgc cgaaatcgaa gaacaaacca acccgaaacc gaaagcgggt aagaaacagc
    13681 tcaaccaaga ccagctcaac accaaaaagc tgatgctgga tttaatcgac cgcgtgcgcg
    13741 acgagtccga cggcgaacat cccgtgcgac tggtgttcga accgaaatcc agccgcatcg
    13801 ataccgatac cttcatcaac acgctgatgg cgcaaacttc gctggaaggc aatgtgtcca
    13861 tgaacttggt gatgatgggg ctggacaacc gccccgcgca gaaaaacctg aaaacgattt
    13921 tgcaggaatg gctggatttc cgcaccgtaa ccgtaacacg ccgtctgaaa ttccgtttga
    13981 accaagtgga aaaacggctg cacatcctcg aaggccgtct gaaagtcttt ctgcacatcg
    14041 acgaagtgat taaagtcatc cgcgaatcag atgacccgaa aaccgatttg atggcggcgt
    14101 tcgggctgac cgaaatccaa gccgaagaca ttttggaaat ccgcctgcgt cagttggcgc
    14161 gtttggaagg tttcaaactc gaaaaagaat tgaacgaatt gcgcgaagaa caaggccgtc
    14221 tgaacatcct tttgggcgac gaaaacgaaa aacgcaagct gattgtcaaa gagatgcagg
    14281 cggacatgaa acagttcggc gacgcgcgcc gcacgctggt ggaagaagcc ggacgcgccg
    14341 tgctgacaca aaccaccgcc gacgaaccca tcacactgat tctgtcggaa aaaggctgga
    14401 tacgcagccg cgccggacac aatctcgatt tgagccaaac cgcgttcaaa gaaggcgacc
    14461 gcctcaaaca aaccctcgaa ggcagaacga ttttacccgt cgtcattctc gattcatcgg
    14521 gcagaaccta cacccttgat gccgccgaaa tccccggcgg gcgcggcgac ggcgtgccgg
    14581 tttcatcctt gatcgagctg caaaacggcg cgaaacccgt tgcgatgttg acaggattgc
    14641 cggaacaaca ttatttatta tcaagcagcg gcggctacgg cttcatcgcc aaactgggcg
    14701 atatggtcgg gcgcgtgaaa gcgggcaaag tggtgatgac cgcagacagc ggcgaaaccg
    14761 ttttgccgcc ggttgccgtc tatgcctcct cgttcatcaa ccccgactgc aaaatcattg
    14821 ccgccaccaa tcaaaaccgc gccctcgcct tccccatcgg cgaattgaaa attatggcga
    14881 aaggcaaagg gctgcaaatc atcggattaa acgccggcga atcgatgacg cataccgccg
    14941 tttcttccga gccggaaatc ctgattgaaa gcgaaggcag gcgcggcgca gcgcacaaag
    15001 accgcatccc catctccctg cttgaggcaa aacgcggcaa aaaaggcaaa ctattaccca
    15061 tatcgggcag cctgaaacag ctttcttccc cgaaataaac ccgactctgc acatactatg
    15121 gtaatttcca atacccgcga acttgagaaa ctcaaagacc ggattcccaa tctgatcaac
    15181 atcgcccgca tcgccatcgt cttccccctg ctgattatgc atatcttcgg gttggaggtc
    15241 agcggacatg aaaacctgca cgcttcgtgg acggcgtggg cattttatgt ttggctcgcc
    15301 atttcctgct ggctgatttt cttttccatc atccatccgc attggcaatg gcagtcgctg
    15361 aaaatgccgc gtttcagcgc ggtggcggac atcacgatga tcggcgtgct gacctacctg
    15421 ttcggcggca tcgattccgg cttcggcatc ctgattctgc ccttcgtcgt ctgctcctgc
    15481 ctgctcagct acgggcgtta ccccctgctc tatgccagct acgccgccat cctgctgata
    15541 ttcaacgcca ttgccgacgg cgatatcggc aaatatccgc acatatcgga tgcccgaacc
    15601 gcctcggcaa ccttcatcct cgtcgccgcc tcctatcttt ccgccatctt cacctcactg
    15661 tcggtcagat acatcgacag cgccggccaa ctggcccgcg acaaccacct cgcctaccgg
    15721 cgcatcaaag gcttgagcca aaccgtactc gaacgcgttc aagaagccgt cgtcgtcatc
    15781 aataccgaag ggctggcggt gctgttcaac cggaaggcga aagacctttt ccccgcactc
    15841 gaaatcggac ggcgcgccga cctgtccgat tctgccgccg aactgtggga ccaagcctct
    15901 ccgcacactt tcgaatacgt cctcggcaca ctcggcctga ccgcccgcat ccgcgccgtg
    15961 ccgatgaaca aagagccgga caagctgctt atcctctaca tccgcccgca aagcgaaatt
    16021 caggcagaag ccctgtccgt caaacttgcc gcgctcggac aactgaccgc caaccttgcc
    16081 cacgaaatcc gcaacccgat gtccgccatc cgccacgcca acgacctgct gcgcgaaaat
    16141 atggaagcgg gggcggcaga tccgttcaac gccaaattgt gcaaaatcat cgacggcaac
    16201 gtctgccgca tcgacaaaat gctcgaagac atttcctcac tcaacaagcg caacaaaacc
    16261 gaacgcgaaa ccatcggcct gataccgttt tgggaagaat tcaaacaaga gttcctgctc
    16321 ggccatcccg atgccgccgg ctgcatccgc ttggatatgc agggcaatca cccgaccgcc
    16381 tatttcgatc ccgcccacct gcggcagatt atgtggaatc tcgccaacaa cgcgtggcgg
    16441 cacagccgca aacagcccgg ctcgatttcc gtcaccatcc gccccgcgca aaaaaacacc
    16501 gtctgcatcc tctttgccga ccgcccgaag tgcaggaaca cctgttcgaa cccttttaca
    16561 ccacggcgga aaacggcacc ggcctcgggc tgtatgtcgc ccgcgaactg gcgcacgcca
    16621 atttcggcga tttgacctac ctaccggaag ccaaatgttt cgaactcaca ttaccggaaa
    16681 aaaccaatga ctgaactgca acaccccgtc ctcgtcgtcg atgacgaaac cgacattctc
    16741 gacctgatgg aaatgaccct gatgaaaatg ggcttgcgcg tccataccgc gtcaggcgtt
    16801 gccgaagcca aaaacaagct cgacagccaa cgctattcgc tcgtcctgac cgatatgcgt
    16861 atgccggacg gctcggggct ggaagtcgtc caacacatca acagccgcct gctcgatacg
    16921 ccggttgccg tcatcaccgc cttcggcaac gccgatcagg cacaggaagc gttgcgttgc
    16981 ggcgcgttcg accccgatac catgcagata caggactatc tcgaccaaat cgaacgcgac
    17041 atcatcgaac aaaccctcaa acaaaccgaa ggcaaccgca cgcaggccgc caaacgcttg
    17101 ggcatcagct tccgttccat gcgctaccgt atggaacgcc tcaacatcgg ctgacgacaa
    17161 aacggcatcc gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg gcacgggaaa
    17221 gcgggttcgc cccacgcccg aacggacaca aaacaccatg accgacatcc ttattgacaa
    17281 caccgccacc gaaaccgtcc gcaccctgat acgggcattc ccccttgtgc ccgtttccca
    17341 accgcccgaa caaggcagtt acctccttgc cgaacacgat accgtcagcc tcaggcttgt
    17401 cggggaaaaa agcagcgtca tcgtcgattt tgcctccggc gcggcacaat accggcgcac
    17461 aaaaggcggg ggcgaactca tcgccaaagc cgtcaaccac accgcgcacc ccaccgtttg
    17521 ggacgcaacc gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg ggctggccgt
    17581 taccgccttc gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca tccgccgcgc
    17641 cctcctcaat cccgaaacgc aagacaccgc cgcgcacatc aacctccatt tcggcaacgc
    17701 cgccgaacaa atgcccgcac ttgtccaaac acaaggcaaa cccgacatcg tctatctcga
    17761 ccccatgtat cccgaacgcc gcaaaagtgc cgccgttaaa aaagaaatga cctacttcca
    17821 tcggctcgtc ggcgaagcgc aagatgaagc ggcactcctg cataccgcac gccaaacagc
    17881 aaaaaaacgc gtcgtcgtca aacgcccccg cctcggcgaa caccttgccg gacaagcccc
    17941 tgcctaccaa tacaccggca aaagcacccg cttcgacgtt tacctgccct acgggacgga
    18001 caagggataa cgcccataaa acaagacacc gaaaaatttg ccgttcttat gcaaacgaga
    18061 aaccggtttt tgcgtttcga ctgttttgga taaatcatca caccttaaag tttgtcattc
    18121 ccacggaagt gggaatccga ttcattcagt tttatagtgg tttaaattta aaccactata
    18181 gctgttttcg agtttcaggc aacttccaaa ccgtcattcc cacggaagtg ggaatctaga
    18241 aatgaaaggc aacaggaatt tatcgtaaat gaccgaaacc gaacggacta gattcccgcc
    18301 tgcgcgggaa tgacggggcg ggcggatgcc gtctgaaatt ccgtcattcc cgtaaaaacg
    18361 ggaatctaga acttctgatt tttcagacga cttttgaaca ttgccgctac ccaatgatct
    18421 ggattcccgc ctgcgcggga atgacgaggt ttcaggttgc tgtttcgggt tgctgttttt
    18481 tatggaaatg acgaggtttt agattgcgag aatttatcct ctccgccgtc attcccacgg
    18541 aagtgggaat caggaaatga aaagcaatag gaatttatcg taaatgaccg aaaccgaacg
    18601 gactagattc ccgcctgcgc gggaatgacg gggcgggagg atgccgtctg aaattccgtc
    18661 attcccgtga aaacgggaat ctagaacttc tgatttttca gacgactttt gaacattgcc
    18721 gctacccaat gatttggatt cccgcctgcg cgggaatgac gatgtaaaat tatccgggat
    18781 tcaaaaagac aggctttcac atccgtggga atgactgcgg aaagatgatt tttataatgg
    18841 attaaattta aatcaagaca aggcgacgaa gccgcagaca gtacaaatag tacggcaagg
    18901 cgaggcaacg ccgtactggt ttttgttaat ccactatatt ttgtcataaa aatccgcacc
    18961 ttaatcagtt ggcggttaaa tcaaactttt agggtgcaga ttacttttta tgatttcaga
    19021 cggcattttg acaggccgca aacctatttc ggcaatacca aaaacttaat caataactct
    19081 ttgaacacaa aaccgaacac gcccaagccc aaaaccaaaa acaaaatggc gatgccgaat
    19141 ttgcccgctt tggactcctt gcccaaattc caaacgataa aacccaaaaa aataatcaag
    19201 ccggtcaggc agattttcaa cgcccaatcg gcaaaaaccg cttcatccat atttttttcc
    19261 tattgttgac gtgtatgtca tataagataa gggtttcaga cggcatttgc ggtgtttttc
    19321 cgccgatgcc gtctgaacac gcaatcagcg cgcgagtgcc tgtttcaaat cgtcaatcaa
    19381 atcgccaaca tattccaaac cgaccgacag gcgcaccaat ccggggcgga tgttggcggc
    19441 gagtttttct tcgggctgca tcctgccgtg cgtggttgtc cacggatggg taatggtcga
    19501 gcgcacgtcg ccgaggttgg cggtacggga gaaaagttcg acgccgtcca cgactttcca
    19561 cgcggctgct tggtcggcga cttcaaagcc gatgacgatg ccgccgccgt tttgctgttt
    19621 gcggataagc tccgcctgag gatggtcggg taatccggtg taatacacgg cttgaacctg
    19681 cggctgcgct tgcagccatt gcgcgatttt caaggcgttg tcgaactgtt tttccatacg
    19741 cagagacagg gtttccacgc cgctcaacaa ctgccatgcg ttaaacggcg acattgccag
    19801 cccgcaagag ttgcagtaaa cggcgacctg cgccatcagt tcttccgaac ccgccaacac
    19861 gccgcccatc acgcgcccgt gtccgtctat ggctttggtc gcggaggaaa cggaaatatc
    19921 cgcgccgtgt ttcaaaggct gagagccgac aggcgacagc aggctgttgt ccaccaccaa
    19981 gagcgcgccg atgccgtgcg ccaattccgc caaggcttcc aagtcggcca cttctcccaa
    20041 gggattggac ggcgtttcca aaaacagcag tttggtgttg gctttgacgg cggctttcca
    20101 ttcgtttata tcagtcggcg acacgtgact cacttcgatg ccgaatttgg taacgatgtt
    20161 attgataaag ccgacggtcg tgccgaacag gctgcggctg gaaatcacat ggtcgcccgc
    20221 ctgcaaaaag gtgaaaaacg ccgcctgaat cgcggacatg ccggtcgaag tggcgaccgc
    20281 gcgttccgca ccttccaaag cggcgatgcg tttttcaaag gcggctgtgg tcgggttggc
    20341 ggtacgggta taagtgaagc ctttgatttt ttttgaaaac aaatcggcag cgtgttgggc
    20401 gttgtcccac atgaagctgc tggtcaggaa caatgcctga ttgtgttcgc ggtattcggt
    20461 ttgttctttg ccgccgcgta tggcgagcgt ttgcggatgg agttttttgc tcatcggtga
    20521 ttcctcggtt ttatccgttc ggcaacggag cgtgcgcccg ttgttt
//
LOCUS       DAWKQB010000022        20383 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.022, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000022 DAWKQB010000000
VERSION     DAWKQB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20383
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.022"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(161..532)
                     /locus_tag="WJL14_000604"
     CDS             complement(161..532)
                     /locus_tag="WJL14_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655714.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIEIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(664..1107)
                     /locus_tag="WJL14_000605"
     CDS             complement(664..1107)
                     /locus_tag="WJL14_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655715.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1150..2019)
                     /locus_tag="WJL14_000606"
     CDS             complement(1150..2019)
                     /locus_tag="WJL14_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ7655716.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPVVLSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAAN
                     DRISLTGGIQWLGKQPDRLDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2183..3106
                     /locus_tag="WJL14_000607"
     CDS             2183..3106
                     /locus_tag="WJL14_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7655717.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKVMFYYRETSRESEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3184..3825)
                     /locus_tag="WJL14_000608"
     CDS             complement(3184..3825)
                     /locus_tag="WJL14_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7655718.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(3880..4671)
                     /locus_tag="WJL14_000609"
     CDS             complement(3880..4671)
                     /locus_tag="WJL14_000609"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ7655719.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            4837..4909
                     /locus_tag="WJL14_000610"
     tRNA            4837..4909
                     /locus_tag="WJL14_000610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4870..4872,aa:Thr,seq:tgt)
     gene            4947..5022
                     /locus_tag="WJL14_000611"
     tRNA            4947..5022
                     /locus_tag="WJL14_000611"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4980..4982,aa:Thr,seq:tgt)
     gene            5164..5955
                     /gene="panB"
                     /locus_tag="WJL14_000612"
     CDS             5164..5955
                     /gene="panB"
                     /locus_tag="WJL14_000612"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ7655720.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDICYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6078..6914
                     /locus_tag="WJL14_000613"
     CDS             6078..6914
                     /locus_tag="WJL14_000613"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ7655721.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7079..8917
                     /locus_tag="WJL14_000614"
     CDS             7079..8917
                     /locus_tag="WJL14_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655722.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9057..9638
                     /locus_tag="WJL14_000615"
     CDS             9057..9638
                     /locus_tag="WJL14_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ7655723.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRPVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETQEQCAARIQ"
     gene            9648..10493
                     /gene="ispE"
                     /locus_tag="WJL14_000616"
     CDS             9648..10493
                     /gene="ispE"
                     /locus_tag="WJL14_000616"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ7655724.1"
                     /translation="MSVTDGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARTPTGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSDLYEAYLAEGLSKH
                     PLLSV"
     gene            10501..10576
                     /locus_tag="WJL14_000617"
     tRNA            10501..10576
                     /locus_tag="WJL14_000617"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10534..10536,aa:Gln,seq:ttg)
     gene            10589..10664
                     /locus_tag="WJL14_000618"
     tRNA            10589..10664
                     /locus_tag="WJL14_000618"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10622..10624,aa:Gln,seq:ttg)
     gene            10673..10748
                     /locus_tag="WJL14_000619"
     tRNA            10673..10748
                     /locus_tag="WJL14_000619"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10706..10708,aa:Gln,seq:ttg)
     gene            10915..11898
                     /locus_tag="WJL14_000620"
     CDS             10915..11898
                     /locus_tag="WJL14_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ7655725.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            11965..12537
                     /locus_tag="WJL14_000621"
     CDS             11965..12537
                     /locus_tag="WJL14_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ7655726.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12662..13831)
                     /locus_tag="WJL14_000622"
     CDS             complement(12662..13831)
                     /locus_tag="WJL14_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ7655727.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNRPTAHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            13979..15505
                     /gene="ilvA"
                     /locus_tag="WJL14_000623"
     CDS             13979..15505
                     /gene="ilvA"
                     /locus_tag="WJL14_000623"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ7655728.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEQEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15562..16635)
                     /locus_tag="WJL14_000624"
     CDS             complement(15562..16635)
                     /locus_tag="WJL14_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ7655729.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQIRAKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELNLIPKQVYVFSQNELIEYSI"
     gene            complement(16632..17492)
                     /gene="cysW"
                     /locus_tag="WJL14_000625"
     CDS             complement(16632..17492)
                     /gene="cysW"
                     /locus_tag="WJL14_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ7655730.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(17870..18706)
                     /gene="cysT"
                     /locus_tag="WJL14_000626"
     CDS             complement(17870..18706)
                     /gene="cysT"
                     /locus_tag="WJL14_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ7655731.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGASRWTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            18845..18952
                     /locus_tag="WJL14_000627"
                     /pseudo
     CDS             18845..18952
                     /locus_tag="WJL14_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            18997..19329
                     /locus_tag="WJL14_000628"
     CDS             18997..19329
                     /locus_tag="WJL14_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ7655732.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            19661..20170
                     /locus_tag="WJL14_000629"
     CDS             19661..20170
                     /locus_tag="WJL14_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ7655733.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT         4888 a         4709 c         5622 g         5164 t
ORIGIN      
        1 atccactata tatatccatt catatagaga aaaattctta tacccaatat aaaacaaggt
       61 ctttcattat attttgaaaa acctagaagc tttgtatcta ttggtttatc aaaattaata
      121 attattgcag aagacatgta ggctaataat cttgatttat ctatatttta ttaattatat
      181 atttaacccc gaagtcaata tagaaattta gtgattttaa agttctctta tcaatttcaa
      241 tctcagggag tacataaaaa tcctcagaat aggcaaactc tatatatgca tcatcattcg
      301 ataataattc aataatattt ttcatttcat ccgaaagaaa tgtaaccatc tcatattgac
      361 atttattttt agataccatt tttgaatatt gattaaataa ttttggatta ctatttttag
      421 taattaatcc ttccggagta ataaaaccac tttgtataaa gtcatcactt atattaacag
      481 ccttccgaaa aaaaatataa tcacactctt tgttatcaat ataaaatctc atactcttta
      541 ataatgaaat ataagaattt ccagaattac aagcaatttg aaaaaacatc attactccta
      601 aaactcataa atagtaagga atatatatat gcatattcct tactattttt ttacaaatta
      661 tgcttatcta tgcttatttc cataaaaagc atgccctcga attttttgac ttgagttctg
      721 aaaatgagaa taatttgaat ggatttcctt ctgtccatgt ctaataacat tttgtcttcc
      781 ccaagcttgg tttgctaccg ctcttgcttg ttgagatgtt cgtgcataga cattagctcc
      841 tcttgataaa gcttgtgctg caacacgctg cgtaacccaa cgctgcgcaa ggaatctgcc
      901 cgcatgcaaa attcctattg ccaccaacgg tgccgcagcc ccttcagttt ctttcatctc
      961 cttttgagaa agctccgcca actgcatcgg cgcatctgcc tgcgtgtgga acacttggtt
     1021 ttcaaatgtc tgattgtcca agccgtttgc cattgcgggg gcaatcatag acagcatcat
     1081 tacggctgag gtgatttgtt ttttcataat aactcctttg gattacaagg ttggaaaatc
     1141 aaaacctgct taaaacgtat gctgtacgcc aaatttcagt tcggaactgc tttgccctga
     1201 aacgttgaaa cgtgcggatg cgtttaaagc cgtggttttg gtgaaaccga aacctgcgcc
     1261 gaaatgggca taggtggatg tgtttcttgc ggattctttt ttgccgtcca gacggtcggg
     1321 ctgcttgccc agccattgga tgccgcccgt gaggctgatt ctgtcgttgg cggcgaagga
     1381 tatattggga ttcagcatcc agtaattgcc tgctttgtat ttggtgttgc ttgaaagggt
     1441 tttgctgccg ttgatacggt aggcagcggt caatgagagg acgacggggt cgatggcttt
     1501 gtaggtggtg gcgccgatga gccaggattt tcccgacgag gctttgttgc gcgatttttc
     1561 gtaaaccgtg ctttcaagaa agctgattag ggcggggttt ttgtcgtctt taaggaaggt
     1621 gtggctgatg ccgagggata cgtcggacat ccgtttgttt cgggttttgc cgttgccgtc
     1681 gagtttgcgt tcttcgtgcc acagatagct gccgctgccg taaatgtcgg tattcccggt
     1741 cagtccgtag cgcaaaccga gcgtgccaac gagcatatcg gtattgctgc cgttttcttg
     1801 gatttcggtc ggaatgggga taaacgaggt tgcgccggtt tggatgtaaa ccggtgcggc
     1861 aagttcggcg cggttgtttt cgctgttcag gtaggtaagg gaagtttcca gtttccattt
     1921 gcccttgtcg gtcattatgt cttcaatcgt caagggcagg tcggcataag cggataaagg
     1981 caggatggcg ggcaaggcgg gcaaaaagat gcgcttcata tttctcctat tgatattaat
     2041 tcttatttac tgataataat gaatatccga taatcttgtc taaataaatt tttaaatcat
     2101 aaaaaacaat atgtttgttt ttgtcaatta tgtttcgata tgccgtctga aaagtttgtg
     2161 aaaaacggtt acaatccgcc gcatgaaaaa acgcaataat cctcttccgc tgttgaacgg
     2221 tgtcaaaccc agttatttgg tgctgccgca tgaaaagcag ttttacgggc tgccgctgct
     2281 gcattttctg tgcatccgct ttccttttgt gggcgcggac gattggcgca ggcggttgaa
     2341 cagcggtttt gtggtcggtt cggatggtgc ggcgttggac gaacattctt tgttcgagcc
     2401 gggtaaggtg atgttttatt accgtgaaac cagccgtgag agcgagccgc gtattccgtt
     2461 tgaagaaaag attttgcata ttgatgagca tttgattgtg gtggacaaac cgcattttct
     2521 gcccgtcatc cccagcggca ggtttttgcg ggaaaccctg ctcacgcgcc tgcgtttgcg
     2581 gcctgaattg cagcatttga atgttgagga cattacgccg ctgcaccgct tggacaagga
     2641 tacggcaggc gtgatgctgc tgtcgcataa tcctgccacg cgcggagcct atcagacgat
     2701 gtttcaaaac aaaacggtat ggaaaacgta tgaggcgctt gcgccgacaa ggacggattt
     2761 gccgtatccg ctcgatgtgg tttcgcgttt ggtgaggggt gagaaatttt ttacgacgca
     2821 agaggcggaa ggcgaaccaa acgcacatac gacggtcgaa ctgattgaaa acagggggga
     2881 attcagcctt taccgcctta cgccgcatac gggcaagaaa caccagttgc gcgtgcatat
     2941 gatgggtttg ggtatgccgc tgttgaatga cgcgctctat cccgtgccgt ctgaagcggg
     3001 cagcgaggat tatcggaaac ctttgaagct attggcaaaa aagattgcgt ttgcagatcc
     3061 tttgtcgggt cgggaaaggg tgttttgcag cggattttgc ctataatgag agggaacgca
     3121 aacccgtatc ggcggatgcc gccgatacgg gttttacatt atcgatgccg tctgaaaggg
     3181 gttttatccg ttcaggcata cgggcgcgaa gcggaggatg gtgtcgcgca ggacgatgag
     3241 tttgccgtga aagaaatgcg tggaaccggc gatggtaatg acgggcaaat cttgcggttc
     3301 cgcccatttc agtgctttcc cgatttcgac gacttcgtct tccgcgccgt gtatcatcag
     3361 cgttttggca acattgggaa cggcggacgg ctcggggcgg ccggtatagt ggcagactgc
     3421 cgcgccgatg agcagcaata aatcgggtat gcgcgcttgt gcggcaaatg tggcgacata
     3481 accgccgaag gagaagccgg ataaggcaaa ttcgggggct tcgggatgtt gggcacgggc
     3541 atagtcgatg acggcgaggc agtcttgcgt ttcgccgcgt ccgtaatcgt gtgtgccttc
     3601 gctgccgcct acgccgcgaa ggttgggcag gtagcagtgg aagccgagtt tgcttaaggc
     3661 tttggcggca gtttggataa ctttgttggt gtttgttccg ccctggaggg ggttgggatg
     3721 attgatgacg gcaacaccgc gtgccggtac ttgttcggac gggatatgga tggtttctaa
     3781 aatgccggca ggaccggata tgtgtatggt ttcgggtttc agcataatga tttcctcttg
     3841 attttccgca tgttccaatg cagccgtgaa gatagttgct taaaggtgga aatgcttgcc
     3901 gttgttgggg ttggacgctt tcaggccggc aagcatacgg tggaagtcca atccgtatgc
     3961 gttgctcagt ttgtcggcac gttttttgag tttgtctatg ttttgctctt tggggctgct
     4021 ttggaaggcc attaccgcga cattgccgtg gctttcggca ggaagttcca agacgcgccc
     4081 ttcaaaaacg ctcaacaacc gttcgatgaa gcgttggtag cgtttgtcgc cgctccacca
     4141 gttggttacg aatatgccgt ctgaagagag tgcgtttcgg cagtctcgga aaaacggttc
     4201 ttcaaccagc gcatcgatga tttgttcgcc gtcgaacccg tccaccaaaa tcacatcggt
     4261 gttgtggcgg aagactttga tatattctgc accgtctgct tcaataattt caaatttctc
     4321 gccctcgaaa ggcaactcaa acaggttgcg ggcaatggcg atgacctgag gattgatgtc
     4381 cacggcggtt tggcgcgtgt cgggcaggta ggtgtctatc cagcgtgcaa acgagccgcc
     4441 gcccaagccg atttgggtga tatgctgcgg aagtgcgtcg gtaaacagca gccagcccat
     4501 catcgcgcgg ctgtaagaga gtaccagctc ggacgggtgg tcgaggttca ttgagctttg
     4561 aacggtgtcg ctgcccaagt gaagcgaacg gatattgcct tcttcggaaa tgccgacttc
     4621 gggaaagccg gattttgcag gacgcaggcg gcggtaggga tgtcttgcca tcggtgtgaa
     4681 cggtcggttt gaaaggcgga tattttatca gaatgccgat gcttgtcctg cttcaaatta
     4741 atttcttttc aaataaatta cttattcgga tttgccgggg ctttcggata aattccttgc
     4801 caaagtgtga agttgcctgc ataattcgcc ttctttgccg gtatagctca gttggtagag
     4861 cacctgactt gtaatcaggg ggtcccgagt tcgactcttg gtgccggcat catttctgct
     4921 gatggtgttg caaaacattt cagtaagccg gtatagctca gttggtagag cacctgactt
     4981 gtaatcaggg ggtcccgagt tcgactcttg gtgccgacac cagatttgac agtccgattg
     5041 tgtaaaacag tcgggctgtt ttgcatttgc ggcacggtcg gtggcagggc tgcgggcatt
     5101 tcactataat agcccgtttc aaactgaaaa ggccgtctga aaagggcggg gtaacaatat
     5161 ctgatgatta ctgtgaacac actgcaaaaa atgaaggcgg cgggcgagaa aatcgctatg
     5221 ctgaccgctt acgaatccag ttttgccgcg ctgatggacg atgccggcgt ggatgtgctg
     5281 ctggttgggg attctttggg aatggcggtt caagggcggc agtcgacgct gcccgtcagc
     5341 ctgcgcgata tatgttatca caccgaatgt gtagcacgcg gtgcaaaaaa tgcgatgatt
     5401 gtcagcgatt tgccgtttgg tgcatatcag cagagtaagg agcaggcgtt tgccgccgcc
     5461 gccgaactga tggctgccgg cgcgcatatg gtcaaactcg aaggcggtgt gtggatggcg
     5521 gaaacgaccg aatttctgca aatgcgcggt attccggtct gtgcccacat cggtctgacc
     5581 ccgcaatccg tgtttgcctt cggcggatac aaggttcagg ggcgcggcgg caaggcgcag
     5641 gcgttgctta acgatgccaa ggcgcatgat gatgcggggg cggcggtcgt gctgatggag
     5701 tgtgttcctg cggaactggc aaaaaaggta actgaatccg tttcctgtcc gaccatcggc
     5761 atcggcgcgg gtgtcgattg cgacgggcag gttttggtga tgcacgatat gctcggcatt
     5821 ttcccgggta agacggcgaa attcgtcaaa aactttatgc aggggcaaag cagcattcag
     5881 gcggcggttc gggcgtatgt tgccgaagtc aaagccaaaa ccttccctgc tgcggaacat
     5941 atttttgcag actgaacaag gtttgctgcc gatgccgtct gaaagccgtt tcagacggca
     6001 ttttgttttg ccgtgcgcgg cgcgtataat cggcgcgttt tgtcgggcag gaagcccgaa
     6061 ggataaggat taccgttatg caaatcatac ataccattca agaactgcgc gcgtggcgta
     6121 aaaatgcggg aacggtggca tttgtgccga ccatgggcaa tctgcacgaa gggcatcttg
     6181 ccctcgttcg cgaagccaaa aaacgcgccg acaacgttgt tgtcagcata ttcgtcaacc
     6241 gcctgcaatt cggtcagggc gaggatttcg acaaatatcc gcgtactttg caacaggatg
     6301 cggacaagct ggaagccgag ggtgttgccg ttgttttcgc gcccgatgag aaagaactct
     6361 atccgaacgt ggaacagcgt tacaacgtcg aaccgcccaa tctgcaaaat gagttgtgcg
     6421 gcaagtttcg tccgggacat ttcctcgggg tggcgacggt ggtgtccaag ctgttcaata
     6481 ttgtcgcgcc ggatgttgcc tgtttcggca aaaaagacta tcagcagctt gccgtgatta
     6541 aaggtttgac cgaggatttg aattttgatg ttgaaatagt gcctgtcgat acggggcgcg
     6601 cggaagacgg tttggcactc tccagccgca accagtattt gagtgcggcg gaacgcgacg
     6661 aagcaccgcg cctgtaccgc gaattaaagg ctgttgccga atccttggcg cagggcagtt
     6721 tggattatgc aggtttggaa aaacgtgccg tccaatccct gacagaatac ggctgggtgg
     6781 tcgattatgt cgaaatccgc cgcgtcgata cgctcgaagt ggcgcgggcg ggagataaga
     6841 aactggtggt cttggccgcc gcccgtctgg ggacgacgcg cctgattgac aatttggaaa
     6901 taaaactccc ttaaaccgca agcgtcggga atgccgtctg aagcggattt gcgtttcaga
     6961 cggcatttat ttttttgaac ggggtttcgc aaatctacaa acgattctgc ttgtgataaa
     7021 gttacgcctg attatatgcc gtctgaaagt tcggacggct gtcggataaa ggaagattat
     7081 gttacccgcc cgtttcacca ttttatctgt gctcgcggca gccctgcttg ccgggcaggc
     7141 gtatgccgcc ggcgcggcgg atgcgaagcc gccgaaggaa gtcggaaagg ttttcagaaa
     7201 gcagcagcgt tacagcgagg aagaaatcaa aaacgaacgc gcacggcttg cggcagtggg
     7261 cgagcgggtt aatcagatat ttacgttgct gggaggggaa accgccttgc aaaaggggca
     7321 ggcgggaacg gctctggcaa cctatatgct gatgttggaa cgcacaaaat cccccgaagt
     7381 cgccgaacgc gccttggaaa tggccgtgtc gctgaacgcg tttgaacagg cggaaatgat
     7441 ttatcagaaa tggcggcaga ttgagcctat accgggtgag gcgcaaaaac gggcggggtg
     7501 gctgcggaac gtgctgaggg aaagaggaaa tcagcatctg gacggactgg aagaagtgct
     7561 ggctcaggcg gacgaaggac agaaccgcag ggtgttttta ttgttggcac aagccgccgt
     7621 gcaacaggac gggttggcgc aaaaagcatc gaaagcggtt cgccgcgcgg cgttgaaata
     7681 tgaacatctg cccgaagcgg cggttgccga tgtggtgttc agcgtacagg gacgcgaaaa
     7741 ggaaaaggca atcggagctt tgcagcgttt ggcgaagctc gatacggaaa tattgccccc
     7801 cactttaatg acgttgcgtc tgactgcacg caaatatccc gaaatactcg acggcttttt
     7861 cgagcagaca gacacccaaa acctttcggc cgtctggcag gaaatggaaa ttatgaatct
     7921 ggtttccctg cacaggttgg atgatgccta tgcgcgtttg aacgtgctgt tggaacgcaa
     7981 tccgaatgca gacctgtata ttcaggcagc gatattggcg gcaaaccgaa aagaaggtgc
     8041 ttccgttatc gacggctacg ccgaaaaggc atacggcagg gggacggggg aacagcgggg
     8101 cagggcggca atgacggcgg cgatgatata tgccgacaga agggattaca ccaaagtcag
     8161 gcagtggctg aaaaaagtgt ccgcgccgga atacctgttc gacaaaggtg tgctggcggc
     8221 tgcggcggct gtcgagttgg acggcggcag ggcggctttg cggcagatcg gcagggtgcg
     8281 gaaacttccc gaacagcagg ggcggtattt tacggcagac aatttgtcca aaatacagat
     8341 gttcgccctg tcgaagctgc ccgataaacg ggaggctttg agggggttgg acaagattat
     8401 cgaaaaaccg cctgccggca gtaatacaga gttacaggca gaggcattgg tacagcggtc
     8461 agttgtttac gatcggcttg gcaagcggaa aaaaatgatt tcagatcttg aaagggcgtt
     8521 caggcttgca cccgataacg ctcagattat gaataatctg ggctacagcc tgctttccga
     8581 ttccaaacgt ttggacgaag gcttcgccct gcttcagacg gcataccaaa tcaacccgga
     8641 cgataccgct gtcaacgaca gcataggctg ggcgtattac ctgaaaggcg acgcggaaag
     8701 cgcgctgccg tatctgcggt attcgtttga aaacgacccc gagcccgaag ttgccgccca
     8761 tttgggcgaa gtgttgtggg cattgggcga acgcgatcag gcggttgacg tatggacgca
     8821 ggcggcacac cttacgggag acaagaaaat atggcgggaa acgctcaaac gtcacggcat
     8881 cgcattgccc caaccttccc gaaaacctcg gaaataatgc aggtccatcc tttcagacgg
     8941 cataaggttt gccgggaagc cggggcattc gggcaaacgg cacgcagttc gcacgcgttt
     9001 tgcacggcac accgaaccca tcggccggca ggatggcatc cgttaaggaa attctgatga
     9061 aacacactgt atccgcatcg gtcatcctgc ttttgaccgc ttgcgcgcaa ttacctcaaa
     9121 ataacgaaaa cctgtggcag ccgtccgaac acacccgcag tttcacggcg gaagggcggt
     9181 tggcagtgaa agcggaaggg aaaggttcgt atgcaaattt cgattggaca taccaaccgc
     9241 ccgtggaaac catcaatatc aacacccctt tgggcagtac gctcgggcag ttgtgtcaag
     9301 acagggacgg cgcattggca gtggacggca aaggaaatgt ctatcaggcg gaaagtgcgg
     9361 aagaattgag caggcagctg gtcggtttca aactgccaat ccaatatctg catatctggg
     9421 cagatggcag gcctgtggcg ggcgcgcctt accgcatcct gccggacggc atattggaac
     9481 aatacggttg gactgtcggc agaaccgccg acagtggggg gcaagtccga acgttgcaac
     9541 tgaataacgg aaatttgaac atcaggctgg ttttcaccga gattggtatg ccgtctgaaa
     9601 ccgaaaccca agaacaatgc gcggcacgca tacagtaagg tagatatatg agtgttacgg
     9661 acggacggca ggcgtttccc gcacctgcaa aactgaatct cgatttgagg attaccggca
     9721 ggcgggaaga cggttatcac aatatcgaaa gcatattctg cctgatagat ttgcaggata
     9781 ccgtatattt gaaaccaagg gacgacggca aaatcatcct gcacaatccg gttgatggaa
     9841 tgccgcagga ggcggattta agctaccgtg ccgcatcgtt gctgcaaaaa tatgcacgca
     9901 cccctaccgg cgtggagata tggctggaca aaaaaattcc gaccggggcc ggtttgggcg
     9961 gaggaagctc ggatgcggca accgttttgc tggtgttgaa ccgttggtgg cagtgcggtc
    10021 tgacgcagcg gcagctcatt gattcgggcg cggctctggg ggcggacgta ccgtttttta
    10081 ttttcggcaa aaatgcgttt gcgcggggta taggcgacag gctggacgaa atggatattc
    10141 cgaaacagtg gtatgtcatc gtcaaaccgc ccgtccacgt ttccaccgca aaaattttca
    10201 cacacgaagg cttgacacga aattccgcct caagcataat gccgactttc caaaacctgc
    10261 aaccgtttag aaatgatatg caggcagttg tatttaaaga ataccctgaa gtttggaaag
    10321 cctattccga gttgtcccga tatggatttg ccttaatgac aggttctggt gcgtgtgtat
    10381 tcacggcgtg tcaagatagg aatagcgcat acaatatata ccgacaagtt tcagatttgt
    10441 acgaggcata tttggcagag ggtctttcaa aacatccttt gttgtccgta taaacattgt
    10501 tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga aggttcgaat
    10561 ccttcctccc cagccaagcc aaacgagttg gggagtcgtc aagcggttaa gacactggat
    10621 tttgattcca gcatgcgaag gttcgaatcc ttcctcccca gccagccctt tttggggagt
    10681 cgtcaagcgg ttaagacact ggattttgat tccagcatgc gaaggttcga atccttcctc
    10741 cccagccaaa taaaagcgtg taagcctgct tacacgcttt tatttcatag aaataaaaat
    10801 attgaaatgc ctttgtttgt gtcggatgtt gcaggtataa tgtcgggctt ggtacaagca
    10861 gagggaagca ttgtgttttc tgagcggaag ttaaacataa aatcaggtga gaatatggct
    10921 gcgtacgaca gtttgatggt atttacaggc aatgccaatc ccgaattggc acaacgtgtt
    10981 gtcaggcatt tggacatttc tttgggcaat gcttccgtat ccaagttttc agacggcgaa
    11041 gttgccgtcg aactgttgga aaacgtacgc gggcgcgatg ttttcatcct tcagccgacc
    11101 tgtgcgccga ccaatgacaa cctgatggaa atcctgacga tggcggatgc actgaagcgt
    11161 gcttcggcag gtcgtattac cacagccatt ccgtatttcg gctatgcgcg ccaagaccgc
    11221 cgtccgcgtt ccgtccgcgt tccgatttct gccaaactgg tggcaaatat gctgtattcg
    11281 gcagggatcg accgtgtttt gactgtcgat ttgcatgccg accagattca aggtttcttc
    11341 gatattccgg tggacaatat ttatgccacc ccgattctgt tgaacgacat caaacaacag
    11401 cggattgaaa atctgaccgt cgtcagcccg gacatcggcg gtgtcgtccg cgcccgcgcc
    11461 gtggcaaaat ccttgaatgc cgacttggca atcatcgata aacgccgccc gaaagccaat
    11521 gtggcagaag tcatgaacat catcggcgat attcaaggca gaacctgtct gattgtggac
    11581 gatatgattg acactgcaaa tacgctgtgc aaagccgccg tcgccctgaa agagcggggg
    11641 gcggaacgtg ttctagccta tgccagccac gctgtattct ccggagaggc ggtcagccgt
    11701 atcgcctcat ccgaaatcga ccaggtggtc gtaaccgata ccattccttt gtctgaagcg
    11761 gctaaaaact gcgaccgtat ccgtcaggta acgattgccg gtctgttggc cgaaaccgtc
    11821 cgccgcatta gcaatgaaga atccgtctca tatcttttca atgaagaagt gatgacaggc
    11881 agcatgttgc tgccataagc ccgaagccgt cttaagctgg tcgcggccga tgacggcgat
    11941 tttacctaac ttggagtatt taacatgact tatgaaattc aagcttctgt tcgtgaagca
    12001 caaggcactg gtgcgagccg ccgcctgcgt cgcgaaggcc aaatccccgg cattctgtac
    12061 ggtgaaggtc aagagcctgt tgcaatcgct gtggatcaca aaaccgtatt ctacgcattg
    12121 gaaaaagaat ctttccatac tgcgttgatt aagttgtctc tgaacggtga aaccaaagac
    12181 gttatcgtcc gcgatttcca aatgcacccg ttccgccgcg aagttcaaca catcgacttc
    12241 caagctgtga aagcagacca acccgtacgc atccgcgttc ccctgcacat cgttaacgct
    12301 gaaaattccc aagcggtcaa actgcaaggc ggccgcgtat ctctgttgaa cacgactgtt
    12361 gaagtagttg ctttgcctgc caacatccct gctttcttgg atttggattg tgccgaagtg
    12421 gttgccggcg acattctgca cttgtcagac atcaaactgc ctgaaggtgt agaaagcgtt
    12481 tccctgaaac gtaacgaaaa tctggctgtt gctaccgtta ccggtaagaa acgctaattg
    12541 atttcagcag cagggcggtg cgtatgcaat acgtaccgcc ctgttgtttt atgccgtctg
    12601 aaccgtgttt cagacggcat ttctttattt gttggaaaaa cgggatattt gaaacggcag
    12661 attactgccc tgtcagacac gcccaaagcc tttgccaccg gcttcttttt tttacatttt
    12721 ccagtgcgac gatttctttt tcggcaatgg tgtatccgtt ttgtctgatt ttgatttttc
    12781 ctaaaatttg cccttttttt actggggcgg gaatcggctg tatggtttct agaatttgtt
    12841 ctgccatttt cgcttcctta tgtggcagag tgatgtaggc ttctttgagg aagcctgcgc
    12901 ggacggtttt tttgctgcct ccggaaattt ggatttgggc aacggttttg cctttcggat
    12961 atattttggg cgtatcgaag gcttgcaatg cccagttcag cagcttgctg ttgtctgatg
    13021 cgcgtgtttc cgccgattcc gaacccaatg tgatgacaag gatgtgcctg ccgttgccgg
    13081 agtatgacac ggcaaggttg tagccgccgc tttctgtgtg tccggctttc agaccgttta
    13141 cattgttgtc cctatataaa aggatattgc ggttgttttg ctctatattt ttgaatttga
    13201 aagatttgat ggaaaacagc gggtaatatt ccggaaagtc gcgcatcaat gcttcagaca
    13261 gctgggcgag gtctttggcg gtggaaacct gtccttctct actcaagcct gtcggatttt
    13321 tgaatacagt gttcttcatg cccaagcgtc gggcttcttt gttcatttgt tgcacaaaat
    13381 tttcaatcga gccgttgccc agccggccgg caagggttag ggcggcatcg tttgcggata
    13441 gtgcaatcat gccttttaag agtttgtcgg tgctgaccgt atcgccggga cgtacaaaca
    13501 ttctgcttcc ttctgaagcc catgcggatt cgggtatttt taagttttct tcagatcgga
    13561 tatttcccga tttcatgttt ttgaaaacca gatatgcggt catcagttgg gttagtgccg
    13621 ccggttcgac aggggtattg atgtttttgg cggataaaat ctgtttgctt tgaaggtcga
    13681 taacgatgtg tgccgctgtg agggtttcgg gtgtttggaa cgtgggggcg gcgtgtgccg
    13741 tcggtctgtt gggcgcgggc gatgcagccg ttgcgtgaga aacgcctaag atgatggaaa
    13801 gcaggacggg caggatttta tgtgctgtca tgaaatattc taattgtgtg cgtgtttcag
    13861 tctgccgatt atacgcttag ggtgtctgat cgggcggatt tttcttgctt tcgcgccgtc
    13921 ttgggcgtat ggttttgggt tttgcgattt taataaaccg attatcccat attgaattat
    13981 gaacacgccc cttccttatt ccaattacct catccgcatc ctgacggcat ctgtctatga
    14041 tgtggcggtc gaaacgcctt tggaaccggc acgcagcctt tctgtacgtt tgaaaaacaa
    14101 catccttttg aaacgcgaag atttgcagcc ggttttttcg ttcaaaatac gcggcgcgta
    14161 caacaaaatg tccaagttgc cgaaagatgc gctcgcttgc ggcgtgattg cggcaagcgc
    14221 gggcaatcac gcacaaggcg tggcattgtc cgcacagcgt ttgggttgcc gtgccgttat
    14281 cgtcatgccg gagactacgc cgaaaatcaa agtggatgcg gtcaaaagcc gtggcggcga
    14341 ggtggttttg cgcggtgttt catacaacga tgcttacgat tatgcgatgg agctggcgga
    14401 acaggaaggg ctgacctata tcgcaccgtt tgacgatcct gatgtgattg caggacaagg
    14461 gacggtaggg atggaaattg tcagccagca tcccgatcca atccgcgccg tattcgtacc
    14521 gataggcggt ggcggtttgg cggcgggcgt ggcggcattt atcaagcagg tccgtcccga
    14581 aatcaaagtt atcggcgttc agaccaacga ttcctgctgt atgaagcagt cggtcgaagc
    14641 gggtgaaatc gtccatttga aagatgtcgg gctgttttca gacggcacgg cggtcaaagt
    14701 cgtcggaaac gaaaccttcc gtctctgcaa agaacttttg gatgaaatca ttacagtcga
    14761 taccgatgcg gtttgcggcg cggtcaagga tattttcgat gacacgcgca gcattaccga
    14821 gccggcgggc gcgttggcgt tggcgggtct gaaagcctat atcgcccgag aaggcgcgga
    14881 aaaccaaacc ctgattgccg ttaccagcgg tgcgaatatg aattttcacc gtttgcgcca
    14941 cgtttcggaa cggagcgaat tgggcgaggg caacgaaggt atttttgccg ttaccatccc
    15001 tgaagaacgc ggcagcttcc ttaagtttgt caatatattg ggaaatagga atattaccga
    15061 gttcaactac cgctacgggg acgatgaaaa agcgcatatc tttgtcggac ttcaagcggc
    15121 aggcccgcag gatttggcgg ttatcggcag ccggttggat gaggcgggat tgcccaatgt
    15181 cgacctgacc gatgatgaga ttgccaaaat ccatatccgc tatatggtcg gagggcggac
    15241 ggacaaagta gaaaacgagc gtctggtcag ttttgagttt ccggagcgtc cgggggcgtt
    15301 ggcgcgtttt ttgaaccata tgcagggcgg ttggaacatt acgctcttcc attaccgcaa
    15361 ccacggtgcg gattacgggc ggattttggt cggtatcgat gtgccgcctc acgatgccgc
    15421 cgcatttgac ggtttcttgg aaagtctggg atacagctat cacgaggaaa cgcaaaatgc
    15481 cgcgtacaag ctgtttcttg cctgacgctt gaaagcacaa tgccgtctga aagcctttca
    15541 gacggcattg cgctttcatg gttaaatcga atattcaatc agctcgtttt gagaaaagac
    15601 ataaacctgt tttggaatta agtttaattc tttgccttcg gcgattgggt aacgcgcggc
    15661 atcgctgcca gccagcgtga tatgcacgtc ttgtttgccg tgtttcacca aaatatgcgt
    15721 caatgcgccg acggcgtgga ctttttcgat ttcggcacaa atcatcggtg tttcgtgttc
    15781 ggcggcgatc tgccattcgt gcgggcggat atagccggtg gcggtttgtt cctgccattt
    15841 gtattgtgcg tccaatttcc acgcgaagcc gttataatgc cagaagcctt tttcgatgcg
    15901 tccttcaaaa gcgtcggttt cgccgaggaa ctcggtaacg aaggcgtttt cgggtttgcg
    15961 gtaaatggct tcggcactgc cggtttgttc gattttgccg tggttcatca cgacgatttc
    16021 gtcggaaact tcgagggctt cttcttggtc gtgcgtaacc agaatgctgg ttacacccag
    16081 gttgtgatgg atgtcgcgca gccaggtgcg taattctttg cgtactttgg catccaacgc
    16141 gccgaagggt tcgtccaaaa gcaggagttt cggttcgacc gcaagcgcac gggcgagggc
    16201 gatgcgctgg cgttgcccgc cggagagttg gtgcggatag gattttgcca aatgcgagag
    16261 ctgcacgagc ttgagcaatt cttcgacttt ggcgcggatt tgtcctttgg acgggcgttc
    16321 ggacttgggc aatacggtca aaccgaaggc aacgttgtca aacacgttca tatggcggaa
    16381 aagggcgtag tgttggaaca cgaagccgac tttgcgctcg cgcacgtgct tggcggttac
    16441 gtcttgccca tcaaacagaa tcttgccgcc gtcggcgttt tccagtccgg cgataatgcg
    16501 taaaagcgtg gttttgccgc agccggacgg gccgagcagg gaaacgagtt tgccggtggg
    16561 gacgttgagg ttgatgtttt tcagcgcgtg aaaattgccg aagtgtttgt ttaagttttg
    16621 gatggtgata ctcatactgc gttcctttcg gcggcggcga gttttttgtc ttgtaatttg
    16681 gtgatgatgt tctgcaccgc cagcgtcgcc agtgccaaaa gtgccaatac gccggagagg
    16741 gcgaatgcgc cggtgaagtt gtattcgttg tagaagattt cgaccaaaag cgggacggtg
    16801 ttggtttcgc cgcgtatgtg tcccgatacc acgctgaccg cgccgaactc gcccatcgcg
    16861 cgggcgttgg tgaggatgat gccgtagagt aacgcccatt tgatgttggg cagggtaacg
    16921 cgccaaaaca tctgccagcc gcttgcgccg agtatcaatg ccgcctgttc ttcgctgtcg
    16981 ccctgagcct gcatcagcgg gatgatttcg cgcgcgacaa aggggaaggt aacgaacagc
    17041 gtcgccagaa taatgccggg gatggcgaag ataatctgta tgccttgcgc ttcgagccag
    17101 ccacccaatg ccgtgtgcgc gccgaacaat aagacgaaca ttaaaccggc caccacgggc
    17161 gatacggaaa acggcaaatc gagcagggta gtcagcaact gcttgcctcg gaagtcaaaa
    17221 cgggtcagca gccacgccat cgccacaccc aatacagcat tgacgggaac gacaatcagc
    17281 gcggtaatca gcgtcaattt gatggcagac cacgcttcgg gatcgtttaa ggattttagg
    17341 tacaaatccc aaccgccttt taaggcttcg taaaacacgg cgacgagcgg cacgaccagc
    17401 atcagcagca gaaagcccag cgcggcggca atcagcaaca cgcgcagccg gcgcggttcg
    17461 gtcaggttgg gattggcgga atagggtttc atggcggtgg tgttctctgg ttggtttaaa
    17521 atgtttggag gtcgtctgaa aagggattga ggggtttcag acgacctttt tgttcattaa
    17581 gtagtaggtc ggatacttgt atccgacaag gttgctgaat cgcaaatgtt tgtcggatac
    17641 aagtatccga cctacgaatt ctgtgttttg gggagcggat tcgttttcag acgacctttt
    17701 atcttttatg ctaaaagaga aataaaaaag tctagctgcc gtcattcccg cgcaggcggg
    17761 aatccaccac agggcaacag gaaaacagaa aataaataag gcagctgaaa ttcaccaatg
    17821 gattcccgcc tgcgtgggaa tgacggtaac aggtatttca gacgccgcct caacccttcg
    17881 cgcccgaacg cctgcccaac gcccactgca tcacgttcag cgcaaacaga atcacaaacg
    17941 aaaccagcag cataaacaac gccaccgccg acgcgccctg tacgtcgaac tgttccagct
    18001 tgcccgtaat aatcagcggc aggatttcag aaaccatcgg aatattgccc gcgataaaaa
    18061 tcaccgagcc gtattccccc gttgcccgcg caaacatcat tcccgcgccg gtcaagagtg
    18121 ccggtgtgat ttcaggcaag aggacacggc gaaacgtagt ccaacggctt gcgcccaaag
    18181 ttgccgccgc ttcctcatat tcgcccgaca attcttccaa taccggctgc acggcgcgga
    18241 cgataaaggg caggctgacg acgaccagcg caatccaaat gccgacgggt gtaaacgcga
    18301 ttttgatgcc caaaggctcg aaaaaacggc ctatccaacc gttgggcgca tacagggtcg
    18361 ccaacgcgat acccgtaacc gccgtcggca gcgcaaacgg caaatcgacc agcgcgttcg
    18421 ccagaccctt gcccgggaat tcataacgca ccaataccca cgccaccagc gtgccgaaca
    18481 cgacattggt cagcatcgca taaaacgaca tccgcaaact cagccagacg gccgccaaga
    18541 cattgggttc ggtaatcgtg ttccaaaatc cgctccagcc gatttccgcc gccttcgccg
    18601 ccatcatcgc aaacggcaag accacaagca gcgacaggca caatacggtc agaccaaggc
    18661 tgagtttgaa gccgggcagt acgccgggcg ttttgagcgc taacataaaa caatgctgaa
    18721 aataaggaaa aggaaggact actttaacga tgccgtccga aaaacggaaa gaatggaaag
    18781 tttggtgcaa agacgaattt gttataaagc ggttggcagt ttctcaagcg ggcgcgatgt
    18841 tttaaaatat agtggattaa atttaaatca ggataaggcg acgaagccgc agacagtaca
    18901 aatagtacgg caaggcgagg caacgccgta ctggtttaaa tttaatccac tataacgcct
    18961 tgttttgacg gaaaaccatc atataaagga acacttatgc agattttatc ttttcagccg
    19021 gacattgcgg aacgtatgct ggaaggtacg gaaggcgagt cggtcaacga aaacgcacaa
    19081 ttcgtccgta cggacaacgg ctattggatt gcgtggcatg aaggcgtagc ggcactgctt
    19141 gcgcccgata cgccgccggg cattccctgt ttttgggtgg aaggcgcgga aagccttgaa
    19201 gagttgtgcg tcatggtgga acgcggcgag tttgacgaag tggaagagtt tgacggcgat
    19261 gacgacgaat ggctcgaaac ggcacagggt tgcgggcacc acggcgacgc ttgcgcctgc
    19321 ggacattaaa ggcattgcag gcttgccgca aggggggcaa ggctttgccg ttttttaaat
    19381 aatcggtgga ttgcatctgt ttgcaatggg ctgaacgctt ccctttgtat tttattgaat
    19441 aaaaaaactt atcttggtat tataattaag gcagcatcaa ttattttggg atgacaataa
    19501 acgcaaagca ttgatttgcg ccgattgcag acttattata gcaggttgcg gcgcggactt
    19561 aatgggaatt gtttatagag catgatggtc tgaaacatca ttaattaaaa ataccattaa
    19621 tccgatttat atttatttca atttcaatgg aaaaacatca atgacaatga ttttaagcat
    19681 tttaagcctg ttttttatca tcagactgtt atttttagcc gtctctatta aacatgaaaa
    19741 agccttgatt gccaaagggg cgaaacaata cggaaaaacc aattccacgc tgcttgcggc
    19801 agttcatacg ctttattatt tggcgtgttt tgtttgggta tggctttctg acactgcttt
    19861 taatggcata tccttgattg gtacgctgac ggtgatggct tcatttgtga tattgtcatt
    19921 gattattaag cagttggggg agatttggac ggttaaaatc tatattttac caaatcatca
    19981 aattaatcgt tcgtggttgt ttaaaacatt ccgccacccc aattattttt taaacatcat
    20041 acccgaactg attggcatcg ccttattatg tcaagcgtgg tatgttttat tgattggtct
    20101 gcccatttat ttgctggtct tatttaagcg tatccgacaa gaagaacagg cgatggcaac
    20161 acttttttaa cccgtttcat caattatagt ggattaacaa aaaccagtac ggcgttgcct
    20221 cgccttgccg tactggtttt tgttaatcca ctatattccg ccatctctaa gatttacagc
    20281 gatacacggg tgatttaagg aatgcccgaa ccgtcattcc cgccactttt cgtcattccc
    20341 gcgaaagcgg gaatctagga cgcagggtta agaaaaccta cat
//
LOCUS       DAWKQB010000023        19797 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.023, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000023 DAWKQB010000000
VERSION     DAWKQB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.023"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(207..2957)
                     /gene="gyrA"
                     /locus_tag="WJL14_000630"
     CDS             complement(207..2957)
                     /gene="gyrA"
                     /locus_tag="WJL14_000630"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ7655734.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVIMRGKTHIEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3055..3555)
                     /gene="hscB"
                     /locus_tag="WJL14_000631"
     CDS             complement(3055..3555)
                     /gene="hscB"
                     /locus_tag="WJL14_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ7655735.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTSFASEFLMQQMEWRET
                     LMEARAGKDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3637..3858
                     /locus_tag="WJL14_000632"
     CDS             3637..3858
                     /locus_tag="WJL14_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ7655736.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(3818..4138)
                     /gene="iscA"
                     /locus_tag="WJL14_000633"
     CDS             complement(3818..4138)
                     /gene="iscA"
                     /locus_tag="WJL14_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ7655737.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4225..4611)
                     /gene="iscU"
                     /locus_tag="WJL14_000634"
     CDS             complement(4225..4611)
                     /gene="iscU"
                     /locus_tag="WJL14_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ7655738.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(4872..6086)
                     /locus_tag="WJL14_000635"
     CDS             complement(4872..6086)
                     /locus_tag="WJL14_000635"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ7655739.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(6115..6561)
                     /gene="iscR"
                     /locus_tag="WJL14_000636"
     CDS             complement(6115..6561)
                     /gene="iscR"
                     /locus_tag="WJL14_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ7655740.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6851..8023
                     /locus_tag="WJL14_000637"
     CDS             6851..8023
                     /locus_tag="WJL14_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ7655741.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(8172..10946)
                     /locus_tag="WJL14_000638"
     CDS             complement(8172..10946)
                     /locus_tag="WJL14_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ7655742.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQIHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEK"
     gene            complement(10936..12732)
                     /locus_tag="WJL14_000639"
     CDS             complement(10936..12732)
                     /locus_tag="WJL14_000639"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ7655743.1"
                     /translation="MADKAPKKAKRFILNAVIASQPASQLYTKLTRKRQEIFFNQTLA
                     FDEIDRLFDAKAFSKFSRHTADGKQAVGEIKRHSDGTPAENLIIKGNNLIALHSLATQ
                     FKGKVKLIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIV
                     ISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKD
                     KSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMFYPFLVKNNTV
                     STITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWGRWRWGYSIKN
                     KARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFG
                     EKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIE
                     TLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILACEDSDGIKTL
                     FEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDD
                     EQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            complement(12647..13090)
                     /locus_tag="WJL14_000640"
     CDS             complement(12647..13090)
                     /locus_tag="WJL14_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655744.1"
                     /translation="MKTDIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRSNSQPASQPIIH"
     gene            complement(13307..14227)
                     /gene="truB"
                     /locus_tag="WJL14_000641"
     CDS             complement(13307..14227)
                     /gene="truB"
                     /locus_tag="WJL14_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ7655745.1"
                     /translation="MTNKPAKRPVNGVLLLDKPEGLSSNTALQKTRHLFRAEKAGHTG
                     VLDPLATGLLPVCFGEATKFAQYLINADKAYTATLKLGEASSTGDAEGEIVATARADI
                     SLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDIA
                     EFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEAL
                     ANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYTE
                     IGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(14285..14809)
                     /locus_tag="WJL14_000642"
     CDS             complement(14285..14809)
                     /locus_tag="WJL14_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655746.1"
                     /translation="MNNLIPEHLAAYAHSDSLQIEGVHRCFSLSCQGRDTFHIRYYGE
                     PFDGLMTDTDKAPVKIVAVEAVSGDEIVLFDGAEHGYNAMFCDKYSPNQKQNRTLTDL
                     DEYTYRVLIHLYYNMDYEDEYEDFVNSEGQVPLIDGRIISFDSLKRNGFDAISVDLID
                     EKHSVRELLNEELS"
     gene            complement(14820..15191)
                     /gene="rbfA"
                     /locus_tag="WJL14_000643"
     CDS             complement(14820..15191)
                     /gene="rbfA"
                     /locus_tag="WJL14_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ7655747.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTVLNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            15382..16626
                     /gene="clpX"
                     /locus_tag="WJL14_000644"
     CDS             15382..16626
                     /gene="clpX"
                     /locus_tag="WJL14_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ7655748.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(16691..17884)
                     /locus_tag="WJL14_000645"
     CDS             complement(16691..17884)
                     /locus_tag="WJL14_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ7655749.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGSEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            18288..18788
                     /locus_tag="WJL14_000646"
     CDS             18288..18788
                     /locus_tag="WJL14_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655750.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDARDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            18873..19427
                     /locus_tag="WJL14_000647"
     CDS             18873..19427
                     /locus_tag="WJL14_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655751.1"
                     /translation="MKKIIASALIATFALAACQDDAQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
BASE COUNT         4629 a         4613 c         4992 g         5563 t
ORIGIN      
        1 agttcaatga gtttattttg tttataccgg cttagacgac tttttctcat aggggcaact
       61 ctaacttaat ttgaatttcc ctagttatct aggacagccc cttgttttta attgactata
      121 atccgctata ttgtgagaag ctggatgacg gatgagggat aaaataaatg ccgtctgaat
      181 cttcagacgg catcgggaga tgattttcag ttctcgactt ccggttcggt tacattggaa
      241 attacagaag cgtcggagag ttcggattcg tcttcggcaa cacgttccag cgataccaag
      301 gtttcgcctt cgtccaagtt aatcagtttc acgcctgctg cggcgcggcc ggtttcgcgg
      361 atttgttcga ctttggtgcg gataagtacg ccgccgctgg taatcagcat caaatcgtcg
      421 gtttcgccga ccaaggttgc ggcgaccaaa tcgccgtttc gctcgccggt gttaatggca
      481 atattgcctt gcccgccttt gtttttgcgg ctgtaatcgg caatcggggt gcgttttccg
      541 tatccgttgg cggtggcggt taaaacttgc aaaccgcttt cttcggtttc aggggcgaag
      601 gtaatcaggc tgacgatttt gccgtcggca ggcaggcgca taccgcgcaa accgccgctg
      661 ccgcgaccgg acgggcgaac accgtgtttg ccgctcggca gtgcgttttc gctgtcggcg
      721 gtttcatctt cgatgccgtc tgaaatttcg gtttcgatgt cggcatcttc cgcttcgtcg
      781 ttgccggatt tttcccagta ttcgttgaag cggatggctt tacctaagtt ggagaacagc
      841 atgatgtcgt ccgcaccgcc tgtttgcgca gcgccgacga ggtagtcgcc ttctttgagc
      901 gcgatggctt taatgccttg ggcgcggacg tttttaaagg cggaaagttg gacttttttc
      961 accagtccct gcgcggtggc gaagaagacg tattggtctt cggggaactc gcgtactgcc
     1021 agaatcgcgc tgactttttc gccttcttcc aactggatga cgttgttaat cggacggccg
     1081 cggctgttgc gtccgccttc gggcagtttg taaaccttaa tccaatgaca cttgcccaaa
     1141 ttggtaaagc acatcaaata atcatgcgtg ttggcaacaa acagggtttc gataaagtct
     1201 tcgtctttgg tggcagccgc ctgtttgccg cgcccgccgc gacgctgcgc ctgatagtcg
     1261 gtggtcggct gggttttgat atagccgcca tgtgtcaggg taacgaccat ttcgcgttgc
     1321 ggaatcaggt cttcatcggc aatgtcgcca ccgaacgggt tgatttcgct gcggcgttcg
     1381 tcgccatagt tggttttgat ttcttccagt tcgtcgcgga tgatttgggt aatgcgttcg
     1441 ggtttggaga ggatatccac aaagtcgatg attttaccca tcaggttttt gtagctttcg
     1501 acaatttctt cttgatcgag gccggtcagg tttcgcaggc tcatgcgtaa aatagcatct
     1561 gcctgaatct cgctcaggta ataaccttgt tctttcaagc cgatgtttgc agccaatcct
     1621 tccggacgca tcatttccaa atccagaccg gaacgcgtca gcatttcttc aacgaggctg
     1681 ctgcgccaag ggtgcgcaag cagtttgtct ttggcctcgg ctgcgttggg cgattctttg
     1741 atgagcttga tgatttcatc gatattggac agtgcgacgg ctttgccttc ggcaatatgc
     1801 ccttcatggc gtgccttctt cagccggaaa agcgtacgtc gggtaacgac ttcgcggcgg
     1861 tggcgcagga attcggagag aatctgtttc aggttcaaca ggcgcggttg tccgtcgacc
     1921 aaaaccacca tattgatgcc gaaactgtct tgcagcggag tcagtttgta gagttggttt
     1981 aagacgactt cggcattttc gttgcgtttc agctcgataa cgacgcgcat accggatttg
     2041 tcggattcgt cgcggagctc ggaaatgcct tccagtgttt tttcccgaac caaatcgccg
     2101 attttctcga ccagcttggc tttgttgacc tgatagggga tttcgtcgat aacgatggct
     2161 tcgcgttcgc cgtttttgcc tatgggttcg atatgggtct taccgcgcat aatgacgcgg
     2221 ccgcggcctg ttttatagcc ttcgcgcacg ccgctcaagc cgtagatggt tgccccggtc
     2281 gggaagtcgg gggcttggat aatgtcgatc agttcgtcga tttcggtgtc gggtgcatcg
     2341 agcaggcgca ggcaggcatt gacggtatcg gaaaggttgt gcggcgggat attggtcgcc
     2401 atgccgacgg cgatgccgga cgagccgttg acgagcagtg tggggaaacg ggtcggcagt
     2461 acaagcggct cgtgttcgct accgtcgtag ttcgggccga aattgacggt ttcttcctca
     2521 atgtctgcca gcatttcgtg ggaaattttc gccatgcgga tttcggtgta gcgcatggct
     2581 gcggcggcaa gcccgtccac cgatccgaag ttgccctgtc cgtctatcag cacataacgc
     2641 atagcgaaat tttgcgccat acggacgatg gtgtcgtata cggcggtatc gccgtggggg
     2701 tggtatttac cgatgacgtc gccgacgatg cgcgccgatt ttttgtaggc ggcattccag
     2761 ttgtttttca attcgtgcat cgcgtacagt acgcggcggt gtaccggctt gagaccgtcg
     2821 cgaacgtccg gcagcgcgcg cccgacaatg acgctcatgg cgtagtcgag atagcttttg
     2881 cgcatttcgt cttcaaggct taccggcagg gtttcgaggg cgaatttgtg gtcgtggcgg
     2941 atggttgcgt cggtcatggt ttcaatgttt cgtatggcaa aaaattgttg cttattttag
     3001 catattttga cgcggaacgg tgcggcggtt acgccgtctg aaacacggtg cggattataa
     3061 tgccgaggaa atttcgttgc ggagtttgtc gagaaacctg ccttggcgga cttgttgtgc
     3121 ggcggtgtcg taatcttgtc gggcaaacga ctgtttcaga ccgcagaaca gtttttcttg
     3181 ttcgtcgcgg atttcgttgt cgagattttt caaggattca aggtctttgc ccgcccgtgc
     3241 ctccatcagc gtttcgcgcc attccatttg ctgcataagg aattcggagg cgaaagaggt
     3301 atgctccggc gcgtcggcat cgatgcccga tgttttcagc aggtaggcgg cgcggtcgat
     3361 agggtttttc aaggtgcggt aggcatcgtt gatggtggaa gacatcatca ctgcctgctt
     3421 ttgctcaaag gcggaagctg aagcgaattt atcgggatgg aaacgggcgg ccaaggcgcg
     3481 gtaggtttgt tccaagtttt cggtgtcgat atcgaaagcg ggttcaatcc ggaagagggt
     3541 gaaatattgg gacatagtag gatgataaat gtaagatttt ggcagaaaac tgtttttgcc
     3601 ttataatctg cagcttctta aacgaaagga ctgaatatgg gcggcaaagt gcagcacaat
     3661 aaaggcaaaa tacgcgacaa tgctttaaaa gccttagtga aatccgattt gttccggcac
     3721 aaggtggaac ggaaaaggaa aggcaaaggc agctacaaca ggcaggaagc gaaaaaatgg
     3781 cgggacggtt ttgatactgt cccgccattt ttatgtctta aacatggaag ctttcgccgc
     3841 agccgcagga gtctttgaca ttggggtttt caaatttgaa accttcctgc aaaccttctt
     3901 tggtgtaatc gacttgtgtg ccgtccagat aaaccaggct tttcgggtcg atataaatgc
     3961 gtgcgccgtg tccttcaaaa atcaggtcgt cgccatcggc ttcatcgaca aattcaaggt
     4021 tgtacgccat ccccgagcag ccgctggttt tcacacccaa gcgtacgccc aagcctttgc
     4081 cgcgtttggc gagatagtca ttgatgtgtt ttgcggcatt ctcggtaagg gtaatcatat
     4141 ttcttccttg ttgtatcgcc ccggacagac ctgaagcggc gggtggttcg gacggcattg
     4201 cgggacgatg ccgtctgaag ggctttatct gttttcctga cgtttgcggt agtcggcaac
     4261 ggccgctttt accgcatctt cagccaagat ggagcagtgg atttttaccg gcggcaattc
     4321 caactcctcg gcgatttcgc tgtttttgat tgccagcgcg tcatccaggc ttttgccttt
     4381 aacccactcg gtaatcaggc tggacgaagc gatggcggaa ccgcagccgt aagttttgaa
     4441 tttcgcatct tcgatgatgc cttcatcgtt cactttgatt tgcaggcgca tcacgtcgcc
     4501 gcaggccggt gcgccgacca tgccggtgcc gacagattcg tcgtttttgt cgaaagtacc
     4561 gacgttgcgg ggattttcgt agtggtcgat tactttatcg ctgtatgcca tgatgtggtt
     4621 tccttaatgt tttgatgatt taagtggttt gtttgccggt tttcagacgg cctgagttag
     4681 attttgcacg ctccgccttc acagccgtcg tcgttttccg aatcgacgcc gccttccacg
     4741 gtaacgccgt cgaaatcgtc aagcaggttg tcgaggttgt ccaaatctaa atcttgttcg
     4801 ttcatatttt tctcttgatt gctttgtttt tggaatgccg tctgaaggtt cggatggcat
     4861 gttggtacgc tttaatgcgc cgcccattca atcgagttca aatcaatccc gtctttgaac
     4921 atttcccaca acggcgacag ttcgcgcagt ttgccgattt tggatttaat cagttcggcg
     4981 gcaaattgca cttcttcttc agtggtcatg cgaccgaagg tgatgcgcag ggacgagtgc
     5041 gccaattcgt catttctgcc caatgcgcgc aggacgtagc tgggttcgag cgaggcggag
     5101 gtgcaggcgg agccgctgga tacggcgagt tctttcactg ccataatcag gctttcgcct
     5161 tcgacgaagt tgaagctgac gttcaggttg ttcggggcgc ggtgttcgag gtcgccgttg
     5221 atatagactt cttcgatgcc ttcgatgcct ttgaggaaga tgtcgcgcag tttgcggtag
     5281 tgcgccatat cttgttcgag ttcttctttg gcgatgcgga aggcttcgcc cataccgacg
     5341 atttggtggg tcggtaatgt gccgctgcgg aagccgcgtt cgtggccgcc accgtgcatt
     5401 tgggcttcga ggcggacgcg cggtttgcgg cggacgtaca gcgcgccgat gcctttgggg
     5461 ccgtacactt tgtgcgcgga catcgagagc aggtcgattt tggcggcttc gacatcgaca
     5521 ggcactttgc cgcaggcttg ggcggcatcg acgtggaaga cgattttacg ttcgcggcag
     5581 atttcgccga tggcgggaat gtcttgcacc acgccgattt cgttgtttgc ccacattacg
     5641 gaaatcagga tggtgtcgtc gcggatggcg gctttgagtt cttccaaatc aatcaaaccg
     5701 ttttcttgaa cgccgaggta ggttacttca aaaccttggc gttcgagttc gcgcattgta
     5761 tctaaaacgg ctttgtgttc ggttttgacg gtgatgaggt gtttgccttt ggttttgtag
     5821 aagtttgccg cgcctttgat ggcgaggttg tcggactcgg tcgcgccgct ggtgaagacg
     5881 atttctttgg ggtcggcatt aatcagggca gcgatgtcgg cgcgggcttt ttcgacggcc
     5941 tcttctgctt cccagccgaa tgcgtggctg ttggaggcgg ggttgccgaa ggtttcggtc
     6001 agatagggaa tcattttttc ggcaacgcgt ttgtcaacgg gggtggtggc ggcgtagtcg
     6061 aggtaaacgg gggttttgac ggtcatggtt tgccctttct ttttcgggtg ttatttaatg
     6121 gatgtgtgta aattggacga cgcggctgcc gtcgccgttg tttttctgtt cgatgatgct
     6181 ttgcagggta acgctgccga ggtagtcgtt gatggttttg tttaaattct cccaaaggtc
     6241 gtgcgtcagg cagggcgcgc cgtggtggca gttggctttg ctgccgcatt gggtcgcgtc
     6301 cagccggtct tcggcggcgg cgatgatttg ggcgatgttg atgcgtgccg ccggtgcggc
     6361 gaggatgtag ccgccgccgg gcccgcgcag gctttcaaca agtccggcgc ggcggagttt
     6421 gccgaacaat tgctcgagat aggagaggga tatgttttgg cgttcgctga tggcactgag
     6481 tttgacggcg ccggtttgcg cgttcatcgc caaatccagc atagcggtaa cggcaaaacg
     6541 ccctttggtg gtcagtctca tggtggtggt tgcccgtgtc ggcttttttt atagtgggcg
     6601 gaattgtcca atatctgagt gtttcagtca agtatggggg cggattgcgc gattggctgt
     6661 ttaaatcggc agattgattg atttttaata ttttgatttt aatatattat ttttgaattc
     6721 taaaccttat gccgcttgaa gtgggagcgg cggcgggtgc agtaaatgtt gaaatgattt
     6781 gccttataat caatattgat atacattatc tatttcatat aaagttattc aggtattcag
     6841 ggggcgacaa atgaaacgtg atttgagcaa aatgacctgc atcgaagact tgcgccgcgt
     6901 tgccaagcgc aaaatgccgc gtatgtttta cgattacatc gattcgggtt cgtggacgga
     6961 aacgacttat cgggaaaata cttcggattt caaagacatc cgcttccgtc aaaaagtatt
     7021 ggtcaatatg gagggacgca gccttgagac taaaatgatc gggcaggatg tgaaaatgcc
     7081 ggtggcgatt gcgccgacgg gttttaccgg catggcacac gcagacggcg aaattttggc
     7141 ggcgcgggcg gcggagaagt tcggtattcc gtttacgttg tccaccatgt ccatctgctc
     7201 gattgaagac gttgccgaga acaccagcgc gccgttttgg tttcagcttt atgtgatgcg
     7261 cgaccgcgag tttatggaaa acctgattaa gcgcgcgaag gatgccaaat gttcggcatt
     7321 ggtattgacc gccgatttgc aggttttggg tcaacgccac aaagacatca aaaacggcct
     7381 gtccgcgccg ccgaaaccga ccatcgccaa tttaatcaat ctggcgacca aaccggaatg
     7441 gtgcatgaaa atgctgaaca cggaacgccg cacgttccgc aatatcgtcg gacacgcgaa
     7501 gaacgtcggc gatttgtctt cgctgtcttc atggacttcc gaacaattcg acccgcgcct
     7561 gagctgggac gatgtggcgc gcattaaaga tttgtggggc ggcaagctga ttatcaaagg
     7621 cattatggaa cccgaagacg cggaaaaagc agcgaaaagc ggcgcggacg cattgattgt
     7681 ttccaaccac ggcggccgac agctcgacga taccgtgtct gccatcaaag ccttgcccga
     7741 catcgtcagc gcagtgggca gcgacatcga agtttggatg gacagcggca tccgcagcgg
     7801 tcaggatatt ttgaaggcgt gggctttggg cgcgaaaggt acaatgatag gtcgggcgtt
     7861 cctgtatggc ttgggcgcgt atggcgaaga aggcgtaacc cgtgcgctgg aaattctata
     7921 taaagaaatg gatatatcta tggcgtttac gggacaccgc gatattcaag atgtcgatgc
     7981 ctcgatttta aggggtaaag attggggtag ggaaacggtt tagatactga aaaatacgat
     8041 gccgtctgaa cacagggttc ggacggcatt aaaatttggc tgtattttaa taatgtgttg
     8101 attaaatatc tgacaaaacg cccttttgta aaggaagttt tgtcaaacgg ggttgcctgc
     8161 aatgcactac tctatttttc aagcgatgcc gcacctaaag ggaatgactc cgtaaattgt
     8221 ccatttttct gatgatcagt aaaaaacggt aaaccgatca aacgataatg cggtgtatct
     8281 ttttgcagga tcttatccat cccatattcc acagtgatgg cttctaaaaa ttcttctttc
     8341 catccatcag tttccactaa atgctcacct ttgggctcaa tgaaaatttg gtaatgcaaa
     8401 aagtcgttca caccattgga agaagatttt tgtttatctt tcagcaataa aataaagtcc
     8461 ggcataaatc cttcaccatc ggcaaagtta ttcaatttaa acacttcttc attacggatt
     8521 aaatgaacgt catatttaga tttcaaatcg cccaaacgcg ctgaaataaa ttgaattaac
     8581 gcttcttcca aacccgttcc agcaaaatta tccatcacat accaatcatt ttgcgtggca
     8641 atagtcgttt ttacatcatc ttttttgacc catttttgtt ttggcgtgcc gaaaatttcc
     8701 cataattttt taggtgtaaa ttcttttgta ccgataaacg gcatatcact ttcattcaga
     8761 tgtttttcaa ccatttccaa gattttcagg cagcctgcaa gtttatcatc cgggcgaacc
     8821 tgtttgtctt gccctaatcc taaaaattca atttgccagt ctttcaacaa gttattttgc
     8881 aattcattgc gattttgaat gttgagtttg ctttgcaggc ggtcaaaatg gaataaagat
     8941 tgactatttt tccctttgat atgtaatgct ttattgaaaa tgtgccgctc catttcactc
     9001 atttttatgg tttgagtaaa attattttgt gtgccgattt gtcgggctgt ttcatcattt
     9061 tcatcggctg taaattgcgt ttcctgcaac aattgattgc cgtgaatttg gaatgaaagc
     9121 gtttgcggat tggcttgcag gctgtctgca ttattgcttt tggcgtttgg gttggggatt
     9181 tttttattcg cccaaattaa caactctcta aaatccttat tatcggcaaa ttcagatttg
     9241 agtttaaatg ttgccaacac cttatcatcg tctttttcag gcaaataacc gtcttttcgc
     9301 aactcatttt tcagttctga aatatagcgg gattgctcat cgtgcgtgta ataaaacaat
     9361 tcttccaaga tacgcaattc gtgttgcatg tcgttgtcaa atttgcgttt attcggctgt
     9421 ttaccttcaa acgcaaatgg aaaataacgc acgccacgac caatcaactg cttttccgat
     9481 acggtagcgg cagccgtttt gcctgatttt ttatttgaac caccgccatt ttgcccttca
     9541 tacaaacgca caatatcaaa caaattcaga acgtcccaac cttcggttaa tctatctacc
     9601 gtaaaaatgg cacggattgg attatcagcc gcttccaaat tgtttagcaa tttttcggtt
     9661 tcgctgtcgg ttttttcagt tttgtttttg ttggtttcgg agttggtaat aatcacatta
     9721 tgtttttgat aattttgttt cacccaattt gccaaatgtg caaactcaaa gccattttcc
     9781 tgcataaatt ttagggcttg ttcggttcgg gttttgcctt gttcgttggc gttgtcgcta
     9841 ttattcaggc tgcctgaaaa cgtatctaaa aactcaaaat catccgcttg tacattttct
     9901 gcccaattta aaaatgccga ataatccgct tttgattcat caatcgtctt acttctaaac
     9961 aacatcacag gcttgaaatt ggcaatgccg tatttcaacg cgatttgatg gcgataccaa
    10021 gcaaataata aagcgtgcaa cacccgctct ttcttattca gcgtactgga taccaaattg
    10081 atttctttgg tataaccttt ttgcaaaaac tcttttaagc caaattttgt gatgattttg
    10141 tccgcatatt tttgttgcac gtcagcattt tcaggcagcg tggcggtaaa ttccaacagc
    10201 acattttggc cgggattgcc atttttattg agcaacaatt ccaaaaccat atgctcccag
    10261 cctttgcgtt caatttcggc attgcctgca ttggctttta attccacttc caaatccaac
    10321 tcgtcttgtt ttttgccttt ggtttgtgcg tttaaatggt gtgcttcatc gcccagcatc
    10381 acaaggttca atttgtgcaa atctgccaat gtggtttggt tttctcgctc ggtgtggata
    10441 tcgttataca gtttttgaat gctggtaaat ttaatttcaa tgccgtctga atgtgggctg
    10501 aacatttcca ctttgcgaat aggaattacc gtatcacctt gcaaaatctt ctcggtaaat
    10561 aaaaattttg cgtgcgccgg gtcaataaaa ttattttccg ttttatccac gatattattt
    10621 tgattgacga aaaacaaaaa atgccgataa cctttttcaa aataatacaa aatcaacgcc
    10681 gccatcatca tcgttttgcc agcacctgtt gccatattga acagcaaatg ggtagggcgg
    10741 tttttgatgt cagggaaatc ctttaatttt gaagcgcggt caaaaatcac aaagttttct
    10801 aaagccgatt tttgccattc aaaaaacgga tattttaaat tgcgttctac aaattcaggc
    10861 agcgcgaaaa aatctgtctg ttctatttct tccagcgttg atttgcggtc ttccacccaa
    10921 tcaaataacg ttttattatt cgccatcttt tttctccgct tgattttttg ctgattgata
    10981 gaattcacgg cttaacgctt tatcgtcatc atttaagcaa cctgcaaatt gctcatcatc
    11041 catttcggat aatgaaacat acatttgatt taaatccaac atttcaagca ccatttgttt
    11101 ttgttcgtct aatgccaagg attgaaattc aggctcttga atgatttgac taaattcatt
    11161 tacgctgacg ttgtatttca agaaatagcg ttcgcataaa ccttcaaaca gcgttttgat
    11221 gccgtctgaa tcttcgcaag ccaaaatttg ttgttttgcg gtttcgttaa atggggcaag
    11281 ttcggcataa acaaactcgc caccaccttg ccaattcacg gctttggaaa taccgccttg
    11341 ctcgccatca atcacttttt tcaagcgttc aacggcaagc gtttcaatat aatccatttg
    11401 ttcaataccg atatattgtc ggttcatttt gtgggcaacg gcggcggttg tgccactacc
    11461 aagatggtag tctaagacga tgtcggattc gttggttgtg atggtaataa aatcttggat
    11521 aagttcttct ggtttaggat ttttaaatgc cttttcacca aaaagatttt tcatctgttc
    11581 agttccatta ccactactgt attcaggttt ataaaaaata gttttaggtt tttttgtagg
    11641 taaatcatct aattcaggtc tttgtttttt atacaaggaa attccatttt tagattggct
    11701 aacaataata tccgtctgca gccttgcctt attctttatt gaataccccc aacgccaacg
    11761 cccccattgt ttatcagcaa taggtaaaat aaaattgtat ccaagatttt cgtatttctc
    11821 ttttagtttt tgaataaaat catcattgaa tacttctaaa tctttattat agatttgaga
    11881 aaattcttca tctgtaattg tactaactgt gttatttttt accaaaaaag gatagaacat
    11941 ttcaggtcta tcttctcttt tatcttcggt tcctgttgct cgcattggag ctcctttttt
    12001 ataaaaccca atttcatctt cttcccattt ctctaaaaaa ttatcttcat ctattggaaa
    12061 ttcataaaaa gtagatttat ctttattttt agcaaagact agtgtgtatt cgtgagtgcc
    12121 tgcaaaagca tattcatcat tattaccttt taaattcata atagtgggta aatttccgat
    12181 aaaattttca aatccaaaaa tttcatctaa caaaatcttt aaataagcat ttccgtcatc
    12241 atcaatggaa ataacaataa tcccatcatc agttaataat tctttggcaa ttgataaacg
    12301 atttttcata aatgtaagcc aagtagataa tttaaagtta gtgttataac caaaactatc
    12361 ttgttgtttc tttttaacaa aataataagg cggatcaata taaatcagct tcactttacc
    12421 tttaaactgc gtggcaagcg aatgcagggc aatcagatta ttgcctttga taatgagatt
    12481 ttcggctggt gtgccgtctg aatgtcgttt gatttcgcca accgcttgtt tgccgtctgc
    12541 ggtgtggcga gagaattttg agaatgcttt tgcgtcaaaa agccgatcga tttcatcaaa
    12601 agcaagcgtt tgattaaaaa agatttcttg tctttttcgg gttaatttag tgtataattg
    12661 gctggctggc tggctggcta ttactgcgtt taaaataaat ctcttcgcct tcttcggtgc
    12721 tttgtccgcc attcaatacg caatctttaa acggaaaatc caacacaata tccgaattgt
    12781 ctttcaaaaa gcggttgccg tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat
    12841 tattgacgct gtgtttgtcc aagaaaaaac gtaaatcctg caatttaaac accaaaacac
    12901 cattcacttc cacaaagaaa tggcgtttta actcatcatt attcaataac aaaccgataa
    12961 tggtcgggtc ggttttttcc accaaatcca acagaatgtt tttggctaaa atggtttttt
    13021 cttcgttcgc ccatactttt tcgtgtgata gcagggcttg ggttaattcg gtttgaatgt
    13081 ctgtcttcat cgttataaat cctcatcagt cattttaatg tgccattctt gtgtaacggc
    13141 aaatggcttt gcaaataaat tctaataaaa tcaatccttt gatgtctttt ctgtttcggg
    13201 gggcgttcgc aaagttgcga taagcgggga gggtgtccga gtgtgtcggg attttgcgga
    13261 aatatcaaca cattattcaa acacagcccg tatttttaac cgtcgttcag gcggcggatg
    13321 ccgccgtgtt catcaggcgc aaggctttca gacggcatat ttccttttga tattctgcca
    13381 aaccgacaaa ttctccaatt tcagtgtaaa cgcgtaccgg cgtgccggaa ggtaggtctt
    13441 cctcgaaacg cggacgttgt ccgcagcgga gcatatggac ggcataatcg tttaaaacgg
    13501 tttggggaaa gtgtgaaacc aatacgtcgc aggatagcag caagctgtcg cgttctgttt
    13561 cgttcaaatt tgccaaggcc tcaagcgtgt ggctttgggc gatggtaaag ccggcggttt
    13621 cggtgcggcg caaggcggtc aggtgggcga acgtgccgat gtgtttggcg atgtcttcgc
    13681 tgagggtgcg gatgtaggtg cctttgctgc aacgtacgtc gatgacggct ttgggcgcgt
    13741 caaattcggc aatatcgatg gcgtaaacgg taatgtagcg cgctttgcgt tcgatgacga
    13801 tgcctttgcg ggcgtattcg tacagcggtt tgccttcgtg cttgagcgcg gaaaacattg
    13861 gcggcacttg gcggatgtcg cccgttaatg ctttgcaggc cgtctgaaat tcggctaagg
    13921 aaatatcggc acgggcggtg gcaacgattt cgccttcggc atcacccgtg ctgctggctt
    13981 cgccgagttt cagcgtggcg gtgtaggctt tgtcggcatt aatcagatat tgggcgaact
    14041 tggtcgcttc gccgaagcag acgggcaaaa gcccggttgc caaaggatcg agcacgccgg
    14101 tatgtccggc tttttcggcg cggaacaagt gcctcgtttt ttgcagggcg gtgttgctgg
    14161 aaaggccttc gggtttgtcg agaagaagaa cgccgttgac cgggcgtttg gcgggtttat
    14221 tggtcatatt aattagggaa tattaatgtt cagaccgtct gaaagggttc ggacggcctg
    14281 cgtgtcagct taattcttca ttcaataatt cacgaacaga gtgtttttca tcaattagat
    14341 caacgctgat tgcatcaaag ccatttcgtt ttaatgagtc aaaactaatg attcgaccat
    14401 caattaaggg gacttgtcct tcagaattga cgaaatcttc atattcatct tcatagtcca
    14461 tattgtaata aagatggatc agaactcggt aagtatactc atccaaatca gttaacgttc
    14521 tgttttgctt ttgatttgga ctatatttgt cgcaaaacat agcgttatag ccatgttccg
    14581 cgccatcaaa caatacgatt tcatcgccgc ttacagcttc tactgccaca atttttaccg
    14641 gagccttatc agtatcggtc atcaatccat caaaaggctc tccatagtaa cggatgtgga
    14701 aagtatctct accttggcag gataatgaaa agcaacgatg cacgccttca atttgcaggc
    14761 tgtcactatg tgcataggcg gctaaatgtt cgggaatcag attgttcatt ttagcagtat
    14821 cagtcttcaa ccggtttttc cgccgccact tgatcgataa gggcggacag gttcaaaccg
    14881 cgttccaacg attcgtcgta tttgaaatgc agctcgggga ttttgaacag cttgatgcgt
    14941 ttggccaatt cgctgcggag gtgtccgcgt gcgtgttcca gcacttcttc cgtaatttcg
    15001 cgcgcatctt ggtttaaaac ggtgtagaac acggtggcgt ggctgtaatc gcgggtaact
    15061 tcgacctcgt tgacggtgat gaagccggcg cgggggtctt tcagtccggt acggacgagt
    15121 tcggcaagct cgcgcatgat ttgttctttg acacggtctt ggcgggcgta gccgcgttgg
    15181 ggttttctca tgaatatcct cagatttcag atttggtttc agacggcatt tttgccgccg
    15241 gggcggttgc gaaagggcgt attatagcct aatcggcggg attttaaggt aatacattga
    15301 taagaacgga tatagttatt atacttggca acccgaacgg cacgtgccgt tttctgtaac
    15361 gcatacattg taaagaaagc catgtccaac gaaaaccgta cctgttcctt ttgcggaaaa
    15421 tccaaatcac acgtcaaaca tttaattgag ggcgaaaacg cctttatctg tgacgaatgc
    15481 gtctcaaact gtattgaaat attgcatgaa gacggaaatg acggcacgcc ttcggaaagt
    15541 gccggagggg agccggaaga atccggcaag ctgcccacgc ccgccgaaat cgtcgccaac
    15601 ctcaacgacc acgttatcgg tcaggaacaa gccaagaaag ccttggcggt gtcggtgtac
    15661 aaccattaca aacgcctgcg ccacccgaaa gccggagcta atgtcgaatt gtcgaaatcc
    15721 aacatcctgc ttatcggccc gaccggatcg ggtaaaaccc tgctggcgca atctttggcg
    15781 cgcaaactgg atgtgccgtt cgtgatggca gatgcaacca cgctgaccga agcaggttat
    15841 gtcggcgaag atgtcgaaca gattattacc aaactgttgg gcaaatgtga tttcgatgtc
    15901 gaaaaagccc agcgcggcat tgtctatatc gacgaaatcg ataaaatttc acgcaaaagc
    15961 gacaacccgt cgattacccg cgacgtatct ggcgaaggtg tgcaacaagc cttgttgaaa
    16021 ctgattgaag gtacagtggc cagcgttccg ccccaaggcg gccgcaagca tccgaatcag
    16081 gaatttatca acgtcgatac caccaacatc ctgtttatct gcggcggcgc gtttgcaggc
    16141 ttggaaaaag tcatccgcca gcgtactgaa aaaggcggta tcggtttcgg tgcgtccgtt
    16201 cacagcaagg acgaaaatgc cgacattacc aagctgttcg gcatcgtcga accggaagac
    16261 ttgattaaat tcggtctgat tccggaatta atcggacgtt tgcccgtgat tgcaacacta
    16321 gaagaactgg atgaggacgc gctgattaat attttgaccg agccgaaaaa tgctttggtc
    16381 aagcagtatc aagccttgtt cggtatggaa aatgtcgagt tggaatttga agaaggcgca
    16441 ttgcgttcca tcgcgcgtca ggcaatggag cgcaaaaccg gcgcgcgcgg cctgaggtcc
    16501 atcgtcgaac gctgcctgct cgatacgatg taccgcctgc ctgatttgca aggcttgaaa
    16561 aaagtggtgg tcggtaaggc agtcatcgaa gagggcaggg aaccggaatt ggtgttcgag
    16621 tcctgatggc aaacaaaacc gcttcggaac gttgaaccac gtttccgaag cggttttttg
    16681 tttttgatgc ctaggggcgg tttgcggcgg caaattccgt cagcacgtgt tccaaacgcc
    16741 gcaaaccttc cgccgtatcc tcatcgttca acagcagcga aggcgcgaaa cgcaatacgt
    16801 ccgcacccgc aaccaaaatc atcacgccgt gtttcaaggc ggcggcggtt atttcggatg
    16861 cgcgtccgcg ataaggcgcg tccaacacgc agccgagcag cagtcccatc ccgcgaacct
    16921 gtgagaacaa gcctgttttc ctgcccaaat ccagcaatgc cgtctgaagt ttctgcccct
    16981 ggctgcggac gttgtgcaag gtttccggcg cgttgatgat gtcgaaggcg cggctgccga
    17041 ccgcgcacgc catcgggttg ccgccgaaag tcgagccatg cgtgcccggt tggaaggcgg
    17101 cggcaatcgt ttctgtcgcc agcatcgcgc cgatcggaaa gccgcagccc aaggcttttg
    17161 ccgaactcaa aatatcggga acaacgccgt aatgttcata ggcaaacagc ctgcccgtat
    17221 gccccatccc ggtttgaact tcgtccaaaa tcaacaacgc attgtgccgg tcgcacagac
    17281 ggcgcgcggt ttgcaaatat tccgcagtgg cgggcaggat gccgctttcg ccctgtatcg
    17341 gctcgatgat gaccgcgcag gtctgttcgc cgacggcagc ttccagcgcg gcaatatcgt
    17401 tgaacggaac gtgcgtaatg ccttgcggca ggggtgcata atccttgctg tatttcggct
    17461 gaccgccgac ggacacggta aacagcgtgc gtccgtgaaa actgttgata caggcgacga
    17521 tttcgctttt tcctccgccg aaacggtcgc gggcgtattt cctcgccagc ttcaacgccg
    17581 cctcattcgc ttccgagccc gagttgcaga aaaaaacctt gtcggcaaaa ctgtttgcaa
    17641 ccaatttttg cgccaattcc tgcgccggcc gcgtcgtata gatattggaa atgtgccaca
    17701 gcttgtgcat ctgcgcgttt aaagcatcga caagggcagg gtggcagtgt cccagcgcat
    17761 tgacggcgat gccgcctgaa aaatcaatat attcacgccc ttccgtatcc caaacgcggc
    17821 tgcctgaagc gcgttcggga atcatcggtg caaaggcgaa attgggggtc agatagtttt
    17881 gcataattgc tcctgattgc ggttttgctc gggattatgc accgtttttc aaagcagata
    17941 aagggaaatt gccgacaata tcgggcagcg gtaaaaataa accgaaatat tttcaatcgg
    18001 atagatggaa aatcaaaaaa aattcgataa aatgcttgag gcgcggcagc gttttgattt
    18061 agaatctccc caatattttt atcttcagac ggcatttcat catgcaaggc aaaaaattta
    18121 caaccgctta ttgctattgg taccacacag acgtgcggta cttttttgcg cttgcttgaa
    18181 atccattcca tacgcaaaac aggccgctcg aaaacggggc ggcttttttg ttaccttcac
    18241 acaggtcgcc cgattcggca acacacgaca gaatcaggaa aatgaccatg aatgcaatgc
    18301 cccaaaccgc cgcacaaaac gaaaccctgc acacttgtag cattattgtc gatgatgcac
    18361 gggatttttc ccccatgctt ctcgatgcag ggcgggacga cacactcatc agcgaatcga
    18421 tgattccgca aatccgcacc gttgccgccc tgatagccgc cgaacggcac gatttcagcc
    18481 gcagcagccc cgccgagttt accgatgccg ccgatttttt tgccgcacgg attttggttt
    18541 tgggcgtgcg ccgcttccat ctggacgtat cgctgcttca gattttgaaa accgccaaca
    18601 gccgcgcccg ccgttttgcc gaaaaacacc gcctgttttt cacacccgcg caagccgaat
    18661 tgagcctgaa caaacacaaa aaccgccgcc tgctgaccgt agaaaccgaa cacgaagtcg
    18721 aaaacaaagg caacccagtc gccaacagcc tcgcatttgt ccgcaaactg cacaccctgc
    18781 ccctgtaaac atcacgattt gccggcagaa tgcttgccaa aattcccgac ggcgatacaa
    18841 taaaaccgat tttccaccca aacaggacac acatgaaaaa aatcatcgcc tccgcgctta
    18901 tcgcaacatt cgcactcgcc gcctgccaag acgacgcgca ggcgcggctc gaacagcagc
    18961 agaaacagat tgaagccctg caacagcagc tcgcacagca ggcagacgat acggtttacc
    19021 aactgactcc cgaagcagtc aaagacacca ttcctgccga agcacaggca aacggcaaca
    19081 acgggcaacc cgttaccggt aaagacgggc agcagtatat ttacgaccaa tcgacaggaa
    19141 gctggctgct gcaaagcctg gtcggcgcgg cggcaggcgc gtttatcggc aacgcgctgg
    19201 caaacaaatt cacacgggca ggcaaccaag acagtcccgt cgcccggcgc gcgcgtgccg
    19261 cctaccatca gtccgcacat cccaatgcgc gcaccagcag ggatttgaac acgcgcagcc
    19321 tccgtgcaaa acaacaggcg gcgcaggcgc agcgttaccg cccgacaacg cgcccgcccg
    19381 ccaattaccg ccgccccgcc atgcgcggtt tcggcagaag gcggtaaatc cggcgtgcca
    19441 atgccgtctg aagggctttc agacggcatt tttgtatttg ttagggacat tgttatgttg
    19501 ccgtttgatt tttagacggc attttgtttc caagagtttg atgtcgggat ggcaattctg
    19561 atttacgtgc gttggcaggg cggcattgtc atgccgccgt tgggttttca gacggcatca
    19621 tatcgaaccg tcaggtcgtg gggtgagcat tcttgcccac cgttgcaagg gcgggatttt
    19681 ttggtgaaca ggaatgccca ccctgcatct gaaacccttc tgcgtcattc ccacgcagga
    19741 aggaatttag atatttgaat ttacagaaat tttcaatcgt tgccaaagcg gaaaagg
//
LOCUS       DAWKQB010000024        19754 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.024, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000024 DAWKQB010000000
VERSION     DAWKQB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19754
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.024"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(203..278)
                     /locus_tag="WJL14_000648"
     tRNA            complement(203..278)
                     /locus_tag="WJL14_000648"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="WJL14_000649"
     CDS             complement(371..730)
                     /locus_tag="WJL14_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ7655752.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="WJL14_000650"
     CDS             complement(753..1397)
                     /locus_tag="WJL14_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ7655753.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1399..1935)
                     /locus_tag="WJL14_000651"
     CDS             complement(1399..1935)
                     /locus_tag="WJL14_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ7655754.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="WJL14_000652"
     CDS             complement(1947..3044)
                     /locus_tag="WJL14_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ7655755.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKMMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="WJL14_000653"
     CDS             complement(3047..4483)
                     /locus_tag="WJL14_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ7655756.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WJL14_000654"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="WJL14_000654"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ7655757.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="WJL14_000655"
     CDS             complement(5473..6120)
                     /locus_tag="WJL14_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ7655758.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WJL14_000656"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="WJL14_000656"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ7655759.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            7495..8571
                     /locus_tag="WJL14_000657"
     CDS             7495..8571
                     /locus_tag="WJL14_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ7655760.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="WJL14_000658"
     CDS             complement(8778..10244)
                     /locus_tag="WJL14_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ7655761.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WJL14_000659"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="WJL14_000659"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ7655762.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLSAPKVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WJL14_000660"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="WJL14_000660"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ7655763.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQIVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="WJL14_000661"
     CDS             complement(13523..13807)
                     /locus_tag="WJL14_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7655764.1"
                     /translation="MKKLLLVAVVSLSAATAFAGDSAERQIYGDPYFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WJL14_000662"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="WJL14_000662"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ7655765.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15824..16525)
                     /locus_tag="WJL14_000663"
     CDS             complement(15824..16525)
                     /locus_tag="WJL14_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ7655766.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="WJL14_000664"
     CDS             complement(16668..17816)
                     /locus_tag="WJL14_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ7655767.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WJL14_000665"
     CDS             complement(17976..18239)
                     /gene="rpsT"
                     /locus_tag="WJL14_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ7655768.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18542..19684
                     /locus_tag="WJL14_000666"
     CDS             18542..19684
                     /locus_tag="WJL14_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7655769.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5083 a         5099 c         4585 g         4987 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gttgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaat aaacgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacacgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caggtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ccgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcattttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgccg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ttcatggtaa cccaggctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgtg ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcgcgaacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttct gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggtgtctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcatca
     4441 gcaataccgc cagtgccaaa ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg tgcactttgc ccgacggcat actgaacgcc gacaaataat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattaaatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ctgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgttc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcacg ccgcaaactg ccgcacttca tccattgtca tatctgccgt
     6181 caaagacaat cgtattctgg atgtaccttt tggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacggaaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattta gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gctgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcggggctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac tacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tagatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag caacaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcacccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcaccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga taaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccaaggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 agatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcgccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtcccg ggtgcgaacg acgggtcgag gcagtctatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac tttaggggcg gacaacacag taaaaggcaa ttttttactg tgttcggtgc
    10801 gtatgccgat ttgtacggaa cgggacgggt cgatgaggcc ttccttgggg gcggtataga
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggg agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acaccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 tccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggcg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattgg caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtgatg attgtcggat gcggcagccc gtgttcgaga caagcctgac tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accggcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt taatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacaatt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatgc cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcatcttt ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacaatgtcg tattcgcggc cgtctttata ggcttccact tccaaaacag
    13621 gtttgcccca atggtcgtcg gcatcgacat cgtgaacctg ataaccgcgc tgttccaaca
    13681 ttttcacggc ttttgtgcgg ttttgttcaa aatagggatc gccgtaaatc tgacgctcgg
    13741 cagagtcgcc ggcaaatgcg gttgcggcac tcaaggaaac aacggcgacc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag ttaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctct gttttcctta tgcttctttg gtttcagccg
    13981 ccttttgacg caggcgcaag ctcaattcgc gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattga gcacgttgtg ttttcgggaa ggcaatcacg tcgcggatag
    14101 attcggcgcc ggtcatcagg gtaaccaaac ggtcgaggcc gaatgcaagg ccgccgtgcg
    14161 gaggtgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgtacgtc tgcgcggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcacgagcc aggcagtttg
    14341 cagggtcgga aaccatcagg tcttcatgac cttcttttgg cgcggtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaagtcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aagtagccgt tatctttgcc gtgttccaag ccgactttga
    14521 tacgcaatgc gccgatggct tcgttcacaa ctttggtttt gtctgcaccg aagaagataa
    14581 tatcgccgtt ttgtgcgccg gtacgcgcga tgatttcctt cagggcattc tcagacagga
    14641 attttacgat tggagattgc aggccgctgt cttcgccgtt ggagaggttg cccacatcat
    14701 ttactttgat gtatgccaga cctttcgcac cgtagatgcc gacaaatttg gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgcaccgt tcggcacacg cagggcaacc acgcggccgc
    14821 ctttcatgtc ggctgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtgaa tttcaagttg atacgcatgt ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaagtccac gcccagcgcg tctttgaaga
    15001 cttgttttgc cataccttcg gtaatgtcca tgatttcatc ctcgttcaag aacgaggttt
    15061 ccaagtcgat ttgagtgaat tcaggttgac ggtcggcacg caggtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg cggcagcgcg aaaaactcgc ctggatgaac gcggctcggc acgaggtagt
    15241 cgcgtgcgcc ttcaggcgtg gagcgggtca gcatcggcgt ttcaatgtcg atgaagcctt
    15301 gtgcgtccag ataacggcga acgcccatag cgacttggta acgcaggcgc aggttgcgct
    15361 gcatcacagg gcggcgcagg tcgataacgc ggttggtcag gcgcacgttt tcgctgatgt
    15421 tttcgtcgtc gatttggaac ggcggcgtag cggcagcgtt caagacttcg atttctttgg
    15481 caaggatttc gattttgccg gaaatcattt tgtcgttggt cgtaccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttacgggc ggaatcggca gcggcaaacg
    15601 cttcgggcgt gtcgggatcg atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccatgg tcgcgtcgac ggtgtaccca gcctttgacg gtaacggttt
    15721 ggtctaagta ttgctcgcta atcagaccgc aatagttggt acgcataaaa tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca acgcttcgtc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gaccggacac gaatgcgatt gtccaaatac ccgattgggg aaacggcacg agtactggtg
    16141 ttttaaacga acggctgctg tcggacagca acgattcgga tacttttttc acactcgaat
    16201 agatggactt cacaaccgga atccgcccca acaagctgtc ccacgcggca agaatctgcc
    16261 ggcccaatac gtttgcggca aataatccgg ttacaaacaa tacggcaatg gcaacgataa
    16321 cgcccagccc cgggatatta aaccccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttgc
    16501 cgccttcggc cgcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccaccccgcc aaaccgccgg
    16621 cggcttcaga cggaacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttgggat tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaacc gcaccctttc cgccatttcg
    17881 ggattttgcc cccgcaatac agaaaacccc gaaaccgtcg ggcttcaggg ttttccgctt
    17941 atcgcgtatc aaccgccttg gcggttttgc aaaaatcaag ccagtgcttt tacttttgca
    18001 gacagacggc ttttgtggcg tgccgctttg tttttgtgga acacgccctt gtcggcgatg
    18061 cggtcgatga ctttgacgga ctcttggtaa accgcttgtg cggcagcttt atcgcctgct
    18121 tcgactgctt tcaatacttt tttcactgca gtgcggaatg cggtacgcag gctagcgttg
    18181 tgggcgcgtt gtttgacgga ctggcgggca cgtttgcgtg cttgtgcgct gtttgccata
    18241 ttggaatctc ctgaaagaaa aataaattct gtaaacgcgc aattttaaag ggggtttaca
    18301 ttatatgcaa gccttttctc tctatccgcc cgtcggacac cctgacagcg caaaaaaacg
    18361 gcaaaatcgt gcggacgttt tccccctctt ttgaaaacaa ggtaattttt taggaaaatc
    18421 aggtcaatat cgctttgagt ttgcacaaat ttactttaca atgcccaaac cttgcgccgg
    18481 ggtgtttatg gggcgcgggg ttttcttttt ttccaacctc tttcatcagg aatctgcccg
    18541 tatgaaaaaa tccgtattag ccgttttggc cgcgctgtct ctggcagcct gcggcggcag
    18601 cgaaaaaaac gccgtgcaac ctcaagcaga cgctgcgtct gctgccaatg ccgaagccgc
    18661 tgccaccgat actctgaaca tctacaactg gtcgaactat gttgacgaga gtacggtcga
    18721 agactttaaa aaagccaaca atctgaagct gacttacgac ttgtacgaaa acaacgaaac
    18781 gctggaagcc aaaatgctga ccggcaaatc cggttacgat ttggttgtgc cgggcatcgc
    18841 cttcctgccg cgccaaatcg aggcgggcgc gtatcaaaaa gtcaacaaag acctgattcc
    18901 caactataaa aacatcgatc ccgaactctt gaaaatgctg gaaaccgccg acccgggcaa
    18961 ccagtatgcc gtcccctatt tctccggcgt gaacacgatt gcgattacgg cgaaaggcaa
    19021 agagcttttg ggcggcaagc tgcccgaaaa cggctgggat ttgctgttca aacccgaata
    19081 cacccacaag ctgaaatcct gcggcatcgc cctgtgggac accccgagcg aaatgttccc
    19141 gattttgctg aactacttgg gcaaagaccc caaaggttcg aatcctgaag atttgaaggc
    19201 ggcggcggaa gtgttgaagt ctatccgtcc ggatgtcaaa cgtttcagcc cgtccatcat
    19261 cgacgagctg gcgcgcggcg acatctgcct ggcggcaggc aacggcggcg atttgaactt
    19321 ggcgaaagca cgttccgaag aagtgaaaaa caacgtcggc atcgaagtgc tgaatccgaa
    19381 aggcatgggc ttctggattg agtcttggct gattcccgcc gatgcgaaaa acgtcgccaa
    19441 tgcccacaaa tacatcaact acacgctcga ccccgaaatc gcggcgaaaa acggcatcgc
    19501 cgtaaccttc gcccccgcca gcaaaccggc gcgcgaaaaa atgcctgccg aactggtaaa
    19561 cacccgttcc atcttcccga acgagcagga tatgaaagac ggtttcgtga tgccgcaaat
    19621 gagcacggat gcgaaaaaac tgtctgtcag cctgtggcag aaaatcaaag tcggcaccaa
    19681 ctgatttgaa gcattaaaaa tgccgtccga acgatgttcg gacggcattt tatattggat
    19741 tgaaatagaa atat
//
LOCUS       DAWKQB010000025        19701 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.025, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000025 DAWKQB010000000
VERSION     DAWKQB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19701
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.025"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(386..2992)
                     /gene="acnD"
                     /locus_tag="WJL14_000667"
     CDS             complement(386..2992)
                     /gene="acnD"
                     /locus_tag="WJL14_000667"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ7655770.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEGIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRILKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLQRKPWVKSSFAPGS
                     KVAEIYLKEADLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRIHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPT
                     DEEIDAIVAEYVKPQQFRDVYIPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLSGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPITCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(3070..3858)
                     /locus_tag="WJL14_000668"
     CDS             complement(3070..3858)
                     /locus_tag="WJL14_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEZ7655771.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLIDERNPLYQMIVSMF"
     gene            complement(4019..5173)
                     /gene="prpC"
                     /locus_tag="WJL14_000669"
     CDS             complement(4019..5173)
                     /gene="prpC"
                     /locus_tag="WJL14_000669"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ7655772.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5259..6134)
                     /gene="prpB"
                     /locus_tag="WJL14_000670"
     CDS             complement(5259..6134)
                     /gene="prpB"
                     /locus_tag="WJL14_000670"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ7655773.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAEACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6760..8070)
                     /locus_tag="WJL14_000671"
     CDS             complement(6760..8070)
                     /locus_tag="WJL14_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ7655774.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(8209..9387)
                     /gene="ftsZ"
                     /locus_tag="WJL14_000672"
     CDS             complement(8209..9387)
                     /gene="ftsZ"
                     /locus_tag="WJL14_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ7655775.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9506..10750)
                     /gene="ftsA"
                     /locus_tag="WJL14_000673"
     CDS             complement(9506..10750)
                     /gene="ftsA"
                     /locus_tag="WJL14_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ7655776.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQLENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10836..11564)
                     /locus_tag="WJL14_000674"
     CDS             complement(10836..11564)
                     /locus_tag="WJL14_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ7655777.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAPDGLPEKESEE"
     gene            complement(11554..12468)
                     /locus_tag="WJL14_000675"
     CDS             complement(11554..12468)
                     /locus_tag="WJL14_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ7655778.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHFQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTGH
                     SLVPKSAAVMGVGFADLCIEILKTAHVG"
     gene            complement(12581..13990)
                     /locus_tag="WJL14_000676"
     CDS             complement(12581..13990)
                     /locus_tag="WJL14_000676"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ7655779.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(14145..15212)
                     /gene="murG"
                     /locus_tag="WJL14_000677"
     CDS             complement(14145..15212)
                     /gene="murG"
                     /locus_tag="WJL14_000677"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ7655780.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYDILLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQQIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVQAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGGLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15216..16541)
                     /locus_tag="WJL14_000678"
     CDS             complement(15216..16541)
                     /locus_tag="WJL14_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ7655781.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVASLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16667..18004)
                     /locus_tag="WJL14_000679"
     CDS             complement(16667..18004)
                     /locus_tag="WJL14_000679"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ7655782.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(18089..18469)
                     /locus_tag="WJL14_000680"
     CDS             complement(18089..18469)
                     /locus_tag="WJL14_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ7655783.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAEQGDADAQ
                     NNLGAMYAEGQGVRQDDTEAVRWFRQAADQGLAQAQFNLGAMYYKGHGVRQDRALAQE
                     WLGKACQNGNQDGCDNDQRLKAGY"
     gene            complement(18596..19678)
                     /locus_tag="WJL14_000681"
     CDS             complement(18596..19678)
                     /locus_tag="WJL14_000681"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ7655784.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4701 a         5468 c         4951 g         4581 t
ORIGIN      
        1 gatttttgtt aatcctctat accatgtcaa ttcagccttt atttcgcggg caaagcccac
       61 gctacaccca ctttccagaa gtacattagg ctgccactcc gaccaaagcc gtttgattcc
      121 gcaaactgtc ggggtgcgcc acaatctacg gctactgtgt tgtgatacgg caaattatta
      181 aaccccgtca ttcccgcgca ggcgggaatc cagatttgtc cgcacagaaa ctcatcggat
      241 aaataaggtt tcctcaattc cactttctgg attcccgcct gcgcgggaat gacgatttac
      301 agtagatgcc tgccatatcg agaattacgt gaatgagcaa aatgttgaac ccgacccacg
      361 ctacgtcttg cttttcagac gacctctaag ccgcgttccc ttccaaaaaa tcctgtgcaa
      421 accgttgcaa tacgccaccg gcttcatata ccaacacttc ttctgcggta tcgaggcggc
      481 aggtaatggg gacttcgacg gtctcgccgt ttttacggtg aatcacaagg gtcaggtcgc
      541 agcgcggtgt gcgttcgccg acaacgtcgt aggtttccgt accgtccagt tgcagggtgt
      601 ggcggttggt acccggtttg aactgcagcg gcaacacgcc cataccgatc aagttggtgc
      661 ggtggatacg ctcgaagcct tcggcaacaa tcgcttccac gccggcgagg cgtacgcctt
      721 ttgcagccca gtcgcggctt gagccttgac cgtagtccgc gccggcaatg atgatgagcg
      781 gctgtttgcg gttcatatag gtttcgatgg cttcccacat gcgcatggtt tggccttcgg
      841 gttcaacgcg tgccagcgaa ccttggcgta cgctgccgtc ttcgtttctc accatttcgt
      901 taaacagttt cggattggcg aaggttgcgc gttgggcggt caagtggtcg ccacggtggg
      961 ttgcgtaaga gttgaagtct tcttcaggca aacccatttt tgccaaatat tcgcctgcgg
     1021 cactgcttgc caaaatcgca ttggatggcg agagatggtc ggtggtgatg ttgtcgggca
     1081 aaatcgccag cggacgcata ccgcttaatg tgcgttcccc tgccagtgcg ccttcccagt
     1141 aaggtgggcg gcggatatag gtagacattg gacgccagtc gtacagcggg cttggtgctt
     1201 tttgcgctgt gccggtgtcg aacatcggga tataaacgtc gcgaaattgc tgcggtttca
     1261 catattcggc aacgatggca tcgatttctt catcggtagg ccaaatgtct ttcaggcgga
     1321 tttctttgcc gtctgcaacg ccgagtacgt cgttttcaat atcgaaacgg atgctgcctg
     1381 ccagcgcgta ggcaacgacc aacggaggcg aagcgaggaa agcctgtttc gcatacggat
     1441 ggatacggcc gtcaaagttg cggttgcctg acaatacggc ggtggcgtac aaatcgcggt
     1501 cgatgatttc tttctggatt ttcggatcca gcgcgccgct catgccgtta caggtggtac
     1561 atgcgaaggc aacgataccg aagccgagtt tttccatttc gggcagcaga tctgcttctt
     1621 tcaaatagat ttcggctact tttgaacccg gggcaaacga agatttcacc caaggtttgc
     1681 gttgcaagcc gaggcggttg gcattgcgtg ccaacagcgc ggcggcgaca acgttgcgcg
     1741 gattggaagt attggtacag gaagtaatcg cggcaataat cactgcaccg tcaggcattt
     1801 ggccgtctga aggctcttcg taaggtttag ccaagccttt gccggccaaa tcggcggtcg
     1861 caaaacgcgc gtgcgggttg ctcgggcctg ccatattgcg cgttacgctg ctcaaatcaa
     1921 atttcaaaat gcgcggataa acggcggttt tcaaggcatc tgcccacaag cctgcggttt
     1981 tggcgtaggt ttccaccaat ttcacctgcg cgtcgtcgcg tccggtcagt ttcaaataat
     2041 caatggtttg ctcatcaata gcgaacatcg cggcagtcgc gccgaactcc ggcgtcatgt
     2101 tggaaatggt cgcgcggtcg ccgatagaca ggcttctcgc gccctcgccg aagaattcga
     2161 caaacgcccc gaccacgcgt tctttgcgca gaaactcggt cagtgccaac acaatatccg
     2221 tcgccgtaat gcccgccttc cgtttgccgt tcagctcaac gccgacaata tcgggcaggc
     2281 gcatcatgga cgcgcgtccc agcattacgg tttccgcttc caatccgccc acgcccacgg
     2341 aaatcacgcc cagcgcatcg acgtgtggcg tgtgcgaatc cgtgccgacg caggtatcgg
     2401 ggaaagccac gccgtttttg acttggacga cgggcgacat tttttctaga ttgatttggt
     2461 gcatgatgcc gttgcccgcc ggaatcacgt ccacattttc aaaagcggtt tttgtccagt
     2521 tgatgaagtg gaaacggtct tcgttacgtc tgtcttcgat ttcgcggttt ttgcggaacg
     2581 catcggggtc gtagccgccg cattccaccg ccagcgagtg gtcgacgatg agctgggttt
     2641 gcaccaccgg attcactttg gcaggatcgc cgcctttttc ggcaatcgca tcgcgcagac
     2701 ctgccaaatc caccaacgcg gtctgcccca gaatatcgtg gcacaccacc cgcgccggat
     2761 accaaggaaa gtcgatttcc tgttttccct caatcagctg acccagccag ctttgcagcg
     2821 tcggcaaatc gactttgtcc gcgcggttga ccaaattctc cgccaaaatg cggctcgtgt
     2881 aaggcagctt gtcgtaagag ccgggtttga tgccctcaca cgccgcacgc gcgtcgtagt
     2941 attccaaatc cgtaccgggc agcggtttgc ggtaacgttg gttggcagcc atgtcggttc
     3001 tcctgtggat ctgtttttct tgtggtttga ggtttcagcc gacgttttga aggggtcgtc
     3061 tgaaagggtt taaaacatcg aaacaatcat ctgatacagc ggatttctct cgtctatcaa
     3121 caatttcaca gccatcgaaa tgctgatgac aatcagcagc ggcttaatca gcttcgaacc
     3181 gaagcggacg gcaaatctcg cacctaaatt cgcaccgaca aacgcaccga ccgccatcgt
     3241 tgccgcaatc gggaaaataa tcgaaccgtg cagcaggaat accgatagcg aaccaagatt
     3301 gcaggcaacg ttcgccaatt tggtgtaaga catcgcgttc aacagcttgc agccgagcaa
     3361 aacaataaag gcaatcagaa aaaacgagcc gacacccggt ccgaacacac cgtcgtaaaa
     3421 acccaaaagt ggtgcaaccg tcagaccgaa cagaaaaaaa gacattctgg ctttgccttc
     3481 cttactgccg tcgagcttgg gcgaaaacac aaaatacagc gcgacaaata tcaacaaaac
     3541 cggcacgacc gccagcagaa tatctttgga aaccaagctg accgataatg caccgaccac
     3601 gccgcctgca aacgatgctg ccgcaatcgg gagacctttc ttccaatcaa tcaaaccttt
     3661 gcgtgcaaaa gaaaccgtag ccgaaaacgt agcagcggct gcttgcagct tgttggtggc
     3721 aattgccgac acgggaggaa tacctgccaa caagagtgca ggcagcgtaa tcaaaccacc
     3781 cccacccgca atcgcatcga taaatccggc aatcatcgca accaaaccca aagcgagtat
     3841 tatgtataaa tcttccatgt ttcttatcct gttacttgca ccaatacagg attatcttct
     3901 tctattaggt taaattttta aacttatttc agacaacctt tccaatcagg caaggtcgtc
     3961 tgaaatcctc agctttgcat accgaaatta aagaacaaac tgctattgca ttcttcaatt
     4021 atcgttcttc aatctccaca aacgccaaat cttcagggcc tgtgtagttt gcgctcggac
     4081 ggatgatttt gccgtctttg cgttgctcaa gaacgtgtgc gctccaaccg gttgtacggg
     4141 aaattacgaa cagcggtgtg aacatagcgg tcggtacgcc caatttttgg taggaaacgg
     4201 cagagaacca gtccagattc gggaacattt ttttctcttc ccacatcacg ctttccaaac
     4261 gttcggcaat gtcaaagagg cgcatatcgc cggtttcttt gctcaaaccg cgtgccactt
     4321 ctttaatgac aacgttgcga gggtcggaaa tggtgtacac cggatgaccg aaaccgatca
     4381 cgatttcttt gcggccgatg cgttcgcgga tgtcggcttc agcttcgtcg gcattgcggt
     4441 agcgtttttg aatatcgtaa gccacttcgt tcgcgccgcc gtgtttcgga cctttcaacg
     4501 cgccgattgc tccggtaatg ctggagtaca tatcagagcc tgtaccggcg atcacgcggg
     4561 cggtaaaggt agaagcgttg aactcgtgtt cggcatacag aatcagtgaa acgtgcatgg
     4621 ctttgatgtg tgattcgctt gggcgtttgc cgtgcaacag ttgcaggaaa tgaccgccga
     4681 tggtctcttc gtcgctttca acctcaatgc gtttgccgtt gtgcgaatat tgataccagt
     4741 acaagaggat gctgccgagg ctggcgatca gtttgtcggc gatgtcgcgc gcttcacttt
     4801 ccggatggct ttcacgttca ggatgaacgc agcccagcat ggatacgccg gtacgcatta
     4861 cgtccatcgg atgggtatgt gcaggcaggc tttccaaaac tttaatcaca cggataggca
     4921 ggccgcgcat ggatttgagc ttggttttat aagcggccag ctcgaatttg ttgggcagat
     4981 ggccgtgaat cagcaggtgg gcgacttctt caaactcgca tttttgtgcc aaatccagaa
     5041 tgtcgtaacc gcgatagctc aaatcgttgc cggtacggcc aacggtacac aaagcggtat
     5101 taccggccgc aacgccagaa agcgcaacgg attttttagg tttgagggtc ggggtttgag
     5161 tagtttcagt catggtattt ctcctttgtg tttttatggg tttcgggttt tcagacgacc
     5221 gatgcggatt tgttgaaagg cagtctgaaa gcggtaaatc atttttgaaa caatttatcc
     5281 agtttttgct cgaaggcatg atagttcaga tgctcgtaca gctcggcacg ggtttgcata
     5341 ctgtccacca ccgccgcctg agtgccatcg cgcataatcg cttcgtaaac attcagagcg
     5401 gctttgcttg ctgcacggaa cgatgacagc ggatacagca ccagcgacac gccgttttca
     5461 gccagctcgc tttgggtata aagcggagtg gaaccaaact cggtaatgtt cgccaacacg
     5521 ggcactttca ccgcatctgc aaattggcgg tacatgttca aatcggtcat ggcttcaggg
     5581 aaaatcatgt ccgcaccggc ttcgacacaa gcttcggcgc gttcgatagc ggcatccaaa
     5641 ccttctaccg ccagcgcatc ggtacgcgcc ataatcacga agttctcatc aacgcgcgca
     5701 tctacggcag ctttgatacg gtcgaccatt tcatctttag atacaatggc tttgttcgga
     5761 cggtggccgc agcgtttttg cgctacctga tcttcgatgt gaaccgctgc aacaccggcg
     5821 cgttcaaagt tgcgaatggt acgggcaata ttgaatgcac cgccccaacc cacatcgatg
     5881 tccaccagca gaggcgtatc cacgttgtcc gtaatgcgtc gtgcgtcgat cagcacatct
     5941 tccattgtgg taatgcccaa atcagggata ccgcaagaac aggctgccac gccgccgccg
     6001 gacagataga tggctttgaa accgctttgg gtggccaatc gtgcaaaata agcattgacg
     6061 caaccggcga cggcaagcgg attcgattct ttcacggctt ggcggaaacg tgctccggca
     6121 gagtgttgac tcatcatatt tctcctttat agactttttt tcagtatgga caggcttcca
     6181 tcacattcgg acggcaaaac acagccatcc gacgcgtcgg gcatcccaat ccaatcatta
     6241 aaaatatatg ggaacaatta tcttattgat atttaaaacg aatcaaagaa aaacagcaga
     6301 ccgttcggaa ttatgcggca aaaccgcaga caagaagaaa acaaggggat tattcagaaa
     6361 aggggaaaac atctgaattg gtttcatagt aatgttcctt tgtagtattg tgtagtttta
     6421 tttttcgcta atctaaaaca caagactgaa aatgtcaaac atttttaagg agactcatta
     6481 cataatttta atattttatt ttaattccat gattaaacta gattaatttt gaattatttg
     6541 tttcaataaa attgtcagga aatcaagaaa aatttttgat aatcaaaatc ctgaaaactt
     6601 ataaaatatt ttatacataa aaacaatgta ttatttttaa gcaatccgat gtattttatt
     6661 atgtatttga tttttattta atttaataaa aattaaatat accggcaaac aaaatgccgt
     6721 ctgaaaacct ttcagacggc atttttaata tcgaatcaat cagcccaaat accagaattt
     6781 cagtgcccac aatacggcaa caatccatac cataggcgga acgtctttgg tgcggcggca
     6841 taaaagttta accacggcat aactgatgaa gccgaaagcg atgccgtctg caatcgaata
     6901 ggtaaacggc atgaagacaa tggtcaggaa tgcgggtgcg gcttccgtca tatcgtccca
     6961 atcgatgtcc ctcgcactgc ggagcatctg cgtgccgaca taaagcaggg cgggcgcggt
     7021 ggcaaaagcg ggaacacttt tcgccaaagg tgaaaacatc aggcaggcga gcatcaatac
     7081 gccgacggta accgccgtca gacctgtccg cccgcctgcc gatacgcccg ccgcactttc
     7141 cacataaggc gtggttgaag aagtacccaa agccgcacct gccacaatag cggtagagtc
     7201 tgcaagcagt gcgcgtttca ggcggggcag cttaccgtcc accagcagtc cggcacgatg
     7261 cgatacaccg accagtgttc cggtactgtc gaacagatcg actaggaaaa agacgaaaat
     7321 cacgctgacc atgctgacgg taaacaaccc tttaaaatcc atctgcataa aagtcggcgc
     7381 aatgctcggc acttcgccga tgatgccgtg aaattcgttc aaacccatca ggctggcaat
     7441 gacggtaatc gtcaaaatgg tgatgatgat tgcgccttga acgcggaaat gtcccaatac
     7501 gaccaccatg gcaaaaccga acagtgccaa caacgcggac ggctgatgaa tatcgcccaa
     7561 gccgaccaag gttgccggat tggcaacgat aatgcctgcg cctttcaggg aaatcagtgc
     7621 caaaaacaaa ccgataccgg cagcaatcga cattttcaaa cccataggca gtgcgttgac
     7681 cagcatttcc ctgactttaa aaaagctgaa caggatgaaa atcagaccgg agatgaacac
     7741 cgcacccaac gcaacctgcc aaggcacgcc cataccctta acgacggcaa aggtgaaata
     7801 ggcattcagc cccatccccg gtgcgagtgc aatcggatag ttgccgacaa aacccataac
     7861 aaaacagccg atggcagacg cgatacaggt agcgacgaat accgccccca tatccatgcc
     7921 ggtctcgccc aaaatcagag ggttgacgat aacgatgtag cacatcgtca aaaaagttgt
     7981 caaacccgcc atcaactcgg tacgcaccgt cgtaccgttt gccttcagct taaaaatccc
     8041 gtccaacagt gtttgttttg aagtgtccat atctgaaagt ctgtatcaag tggaaaaatc
     8101 tgaatccgcc gaccgcaaac cgtacgaaat tgcaacatct tgaaaaacat acctcccaaa
     8161 ccggacaaac cgattcggga ggcagcaggt ttacgggcaa acagcacatt atttgtctga
     8221 attgtgttga cgacgcaaaa tcgcagggat ttcaaagtcg tcaagtacgg actgattgtc
     8281 gaaatccgca gcggtaaggt tcatcgtgcg gataccgcga ttggtgcgga tcataccttc
     8341 gacattgtgg ctttgctcct gtttggacgg agcaaccgct tctacctccc ttgccggaac
     8401 aaaatcgacc gcgccttttt ctttcagacc ggtagcgata atggtaatcc ggatggcatc
     8461 ttcgctcatg gtctcgtctt cagccgcacc gaatttgcat tccaaatcgg gatgcgcgct
     8521 ttggttgacg attttcatga cttcggacaa ctcggacatt ttcaagcaac ccggagcagt
     8581 cgtaatattg accagcacac cgcgcgctcc gtccaaggtt acatcgtcca gcagcggact
     8641 ggaaatggcc tggtcggtcg ccatacgcgc acggtcgata ccttgggcat aacccgaacc
     8701 catcatagcg ataccgcggt tgctcatcac ggttttcacg tcggcaaagt cgaggttgat
     8761 gatttcgctc gggcaagtta ccacttcgga aatgcctgcg accgcatcgc gcaatacatt
     8821 gtcggcggca cggaaggctt cgcgcatcgt tacgtcttca cccaatgcag tcatcagttt
     8881 gtcgttcggg atgatgatca gcgaatcgac gtgttctttc aactgttcca accctgcctg
     8941 tgcgacatgg acgcgcttac cttcatatgc gaacggtcgg gtaaccacgg caacggtcag
     9001 aatgcccaaa gacttggcaa tctcagcaac aaccggcgcg gaaccggtac cggtaccgcc
     9061 gcccataccg gtcgtgataa acagcatatt cgcaccgcga atggcttctt caatggcttc
     9121 ccggtcttcc tgggctgccg cacggccgat atcgggattc gcgcccgcgc ccaaaccgcg
     9181 tgtcagattc gtacccaact ggattctctt cgccgcatgg ttttttgcca gagactgcgc
     9241 atccgtattg gcactgataa actccacacc gcgcacattg ttggcaacca tgttattgat
     9301 tgcattgcaa ccgccgccgc ccaagccgat tactttaatc accgcagggc tgactgccga
     9361 ttctgccacg tcgtaaacaa attccattca aaaactcctg ctcgccccat tcagaggacg
     9421 gtttaaataa attattattc attatataag aagtatcttg ctgctggcaa aatacttctc
     9481 acctgtcaaa cggcaatcca cctgttcaga agctgttttc aatccaccgt ttcaatcttg
     9541 ccaacaaacc gccgccccct tccctctctt gcactgcacc gttttccagc tgcggcaagt
     9601 ttccttccag cttgcatgct gcatgaagca gcccgatggc ggtagaaaag cgcggtgtgc
     9661 ggacgcggtc ggacaaaccg cccatttctt ggggtgcacc ggtgcgtaca ggcagatcga
     9721 agattttttc ggcaaattcc acaatcccgg tcatcatgga cacaccgccg gtcagaacga
     9781 tacccgcatt cagcacttct ttggggaaac ccgatttttg cagctcgccc agcactacgc
     9841 caaaaatctc ctgaatacgt gcgctgatga ttgctgccaa aaccttactg gaaacctgac
     9901 gcgatgtccg gtcacccacg cccggaactt caatcatctc acccaagcct tccgtatcgc
     9961 atgatgccac gccataatgg attttaatgt actcggcggc atcgagaggt gttctcaacg
    10021 atttggacaa atctttggta atcagattac caccggccgg aatgacggac gtatggcgga
    10081 tggcaccgtt catataaacg gcaatatcgg tcgttccgcc accaatgtcg atgacgcata
    10141 cgccgaggtc tttttcatct tcagtcagca ccgcctgccc gcttgccaac ggctgaagca
    10201 tgatctgatc gcttttcaaa ccgcaccgct cgatacattt ttggacattc tgcactgccg
    10261 tacttgcacc ggtaatgatg tgcacccgcg tatccagacg cacaccgctc ataccgatgg
    10321 gctccctcac gccaagttgg gtgtcaataa tgtagtcttg aaccacggca tcgagaattt
    10381 tttgatcggg cgggatattg attgcctttg ccgtttcaat ggcgcggtcg atgtctgcct
    10441 gcgtgacttc cccgtcttta attttaacca caccttgcga attgagactg cggatgtggt
    10501 tgcctgcgat acctgtggta acgtgggtaa ttttggtatc cgccatcagc tcggcatcat
    10561 tgaccgcctg cctgatggct tggacggtgg catcgatatt ggttaccatg cccgcgcgca
    10621 agccccgtga aggagcctgc cccaaaccga cgatgttgat tttgtcgtca tcttgaactt
    10681 ccccgatcag tgcgaggact ttagacgtac cgatatccag tacgctgatg tatctttgct
    10741 gctgttccat tgttcgtctg ctcttaaaac tgattgaaat ttgcgtcgca ccgtttcaga
    10801 cggcacggcc gtaatctgtc cgatacctgt tcccactatt cttcggattc tttttcgggt
    10861 aaaccgtcgg gagcatagcg gactgaaaat ccgtccttat acctcatatc cacataggat
    10921 aaccgatttt tatttttacg caacagatgt tgccacgctt cggtaaaaag ccggaggcgt
    10981 ttcatctcgt tttcccgtcc gagcctgacg gtgatgccgt tgtccaaaac gacaatccac
    11041 gccgaacgtg ccgtataggt catctctttg atgcccaaac cctgttttgc caaaacagtc
    11101 gaaaattcgt cataacggcg gagcatttcg gcagacgttc cttccgcgcc tctgaatacc
    11161 ggcattccgg gtctgtccaa acgggcttca aaaacattgc cttcgccgtc caccaaggca
    11221 tggtcgcccc aacgcgcgac cggcttgcgc tcggtcagga cgacctcaac cgtgtcggga
    11281 aaacggcggc gcaccatgac cgacgcaatc cacggatacc ggcggtaggc ctcctgtgcg
    11341 ccattgatgt ccgtcctcaa aatattccca tggatgtatt ctttcgccaa actgcccaat
    11401 gctttcttat cggaataaac taggttgccc ttcagcgaca cctgcttgac gggcagatga
    11461 ttcgaattgt aaaaccaaac cagcccggac gcagcaagca gcatcgccat catgacaagc
    11521 agccagcgcg tcagccgttc catcgcttcg gcattatccc acatgtgcgg tcttcaaaat
    11581 ttcaatacat aaatcggcaa aacccacgcc cataacggca gcggatttcg gtactaaact
    11641 atggccggtc ataccgggca gggtgttgat ttccaacaga tagagtttgc cgtcggtatc
    11701 tttgaggaaa tcgacgcgca cgcagccttc cgcaccgatt gcctgcgcgc cgcgaaccgc
    11761 cagttcgcgc atcaggcttt cttcggcttc ggtcagatct tccgaaggac attgataaat
    11821 ggtgtcgttg cggttgtact tggcttcgta gtcataaaac tcggtcgcgg ggatgatgtg
    11881 tatgccgggc aggcctttgc cgttcaacac agggcaggaa tattcgccgc cgccgataaa
    11941 ccgttcggca atgatttcgc cctgaaagtg tttcaattct tcgtaaacgc ttttcagacg
    12001 gccttttcct ttgactttta ccacgcctac gctgctgcct tcggccgccg gtttcacaaa
    12061 catcggcagg cccaattttt cttcgacggc atcgaaatca gtgtcgtcgt gcaggacggc
    12121 gaactcggga acgggcaatc ccaatgcctg ccaaatcagt ttgcagcggt atttgtccat
    12181 gccgatggcg gatgcggcga caccgctgcc ggtataggga atgcccaaca gttccaatgc
    12241 accctgaaca gccccgtctt cgccgtaagt accgtgaagg atgttgaatg ccgtctgaaa
    12301 accttgtgcc ttcaattcag acaatggggt ttccttggga tcgaaggcgt atgcgtctat
    12361 gcctttgctt tttaaagcat tcaaaatggc ggtgccgctg tccagcgaga tttctcgttc
    12421 gctggaaaaa ccgcccatca atacggccac tttgccaaaa ttctgcattg tttttgttct
    12481 ttcctgattg ctttatgctt gttgccagag gtcgtctgaa acctgatttg cggtttcaga
    12541 cgacctttat atgatgttcc gtctgtcagg cgggtgtgcc tcaaatctgt ttcgacaatt
    12601 ccagcagcgc ggcggggacg cggttgatgc ttcccgcacc catattcaac acgatgtcgc
    12661 cgtcctgcaa aacgttcaac agcatttcgg gcagatcggc aacgttttcg cagtaaatcg
    12721 gctcgagttt gcccaacacg cggatggcgc gggcaagagc gcgggaatca gcggcggcaa
    12781 tcggctcttc accggcggca taaacttcgg tcagcaccag cgcgtcaacg gtattgagga
    12841 ctttggtaaa gtcttcaaac aaatcgcgcg tgcgggtata gcggtgcggc tggaaggcga
    12901 gtaccaaacg tttttccgga tacgcgccgc gtgcggcgga aagggtcgcc gccatttcga
    12961 cggggtggtg tccgtagtcg tccaccaaga gcgcggttcc accgtttggc aacttgatgt
    13021 cgccgtattt ttggaagcgg cggccgacac cttcaaagcc gagcaagcct ttttggatcg
    13081 cttcaaccga tgcgccgact tccagcgcca cgccgatggc tgccaatgcg ttcagcacgt
    13141 tgtgtctgcc gggcatattc agcacgactt caaacgaccc ctgctcatgt cctttcattt
    13201 gaacatggac ggtgaatttc atttgcgcgc cgacgttttc gatgtcggtg gcgtagatgt
    13261 cggcggtatc gtccaaaccg taagtagcat aaggtttgct cactttgggc aaaatcgcgc
    13321 ggacgtgttc gctgtcaata cacaaaaagg ctttgccgta gaagggcata cggtggatga
    13381 aatcgataaa cgcctgatgc agcttctcaa cactgtgccc gtaggtatcc atatggtctt
    13441 cgtcgatatt ggtaacgacg gacataatcg gtgtcaggtg cagaaaggat gcatccgact
    13501 cgtcggcttc ggcaacgatg tattcgcctt tgcccaagcg ggcgttggtg cctgcggcgt
    13561 tgagtttgcc gccgataacg aaagtcgggt caagtcctgc cgcgccgagg atggaggcgg
    13621 tcaggctggt ggtcgtggtt ttgccgtgcg tgccggcaat ggcgatgccg tcacggaagc
    13681 gcatcaactc cgccaacatc agggcgcgcg gaataacggg aatttgctgc tccaacgcag
    13741 cgacaacttc gggattttct tttttgacgg cggtagaggt aacgacgaca tccgcaccgt
    13801 taacgtgttc tgcggtatgg ccgggataaa cttgaatgcc caggctgccc aaatgctcgg
    13861 tagcggcatt tcgcgcctga tccgaaccgg aaactttaaa acccaaattg tgcaagactt
    13921 cggcgatacc gctcatgccg acgccgccga taccgacaaa atggatgttg gtcactcgat
    13981 ttttcatcat aatgttgcgt tccggtggat tttcgatgcg taaaggcgtt attttaaagg
    14041 gctgaccgtt tgcgcgccat agttttctga caaatatata gcggattgaa ataaaaaaca
    14101 tccatgccgt ctgaacggct tttcagacgg catggttcgg cagtttacgc cgcacacgca
    14161 atcgcggctt cggcaacgtc gtccgcactg tgcggcagtg ccaacgtacg ggcgttttct
    14221 gcccatttga ggcatttttc gcggtttaag ccgccgagaa tctcggcgag tttttccgcc
    14281 gtcaactggg tttgcggcaa cagcaatccc gcctccgcct gcaccataaa acgcgcgttg
    14341 gcggtttgat ggtcatcaac ggcgtgagga tacggcacta acaacgcacc caatcccgcc
    14401 gccgtcaact cggcaatcgt cagcgcgccg gcacggcaaa tcaccaaatc ggcatcgcgg
    14461 taggcggaca ccatatcggt aataaattcc acgcattccg cttgcacgcc cagcgcgtcg
    14521 taatccgcct gcaagctgcc cagcttgccc cgtcccgatt ggtggtacat ctgcggacgc
    14581 gcattgtcgg gcagcaaagc caatgcctgc ggtacggttt tgttcaaaac gtccgcgccc
    14641 aaactgccgc cgaccaccaa aattttcaga cggccttcac gcccttggaa gcgttcggca
    14701 ggcacgggca ggttgctaat atcggcgcgg acggggttgc cgaccaagcc gccttcgtgg
    14761 ctgaacgctt tcggaaaagc gtacaacacc cgcttcgccc agcgcgacag gtggcggttg
    14821 gacaaacctg ccacggcgtt ttgctcgtga atcacaatcg gcacgcctaa taacttcgcc
    14881 gccaaaccgc cgggaaaggt aacgaagccg ccgaagccga tgacgcactc gacacggtgt
    14941 ttgcggataa tctgctgcgc ttcgcggaca gtttgataca aagtaaacgg cagcatcagc
    15001 ttgcgtttga tgccgttgcc gcgcacgcct ttaatcgcca gcgtttcgag caggatgtcg
    15061 tattgcggca cgatgcgctc ttccatcgaa tccttgctgc ccagccaaat tacatgatgg
    15121 ccgcgcgcgc gcaatgaatc cgccaccgcc agcgcgggga aaatatgtcc gcccgttccg
    15181 cccgccatca gcataaaggt tttaccgccc atgatttact ccacccggta accgcgcatt
    15241 ttccggcggt tttcataatc tatacgcaac agcagcatca tgctgatcag catgaaaaag
    15301 actgacgaac cgccatagga catcaacggc agcgtcagac ctttggtcgg caaagcaccg
    15361 atgttcacac cgatattgaa gaaactttgg ataccgatcc aaatgccgat acccgaagcg
    15421 atataggcgt tgaaagtcaa acccaaatcg cgcgactgct tgccgatgga aaacgcccgc
    15481 accaccagcc agccgtaaca gaatatcagc acgcacatac cgaagaaacc gaattcttcg
    15541 gcgatgatgg caaaaataaa atcggtatgc gcttccggca gaaagccgcg tttgctcaaa
    15601 ctcgcaccca aacccatacc gaaccactct ccgcgcccga ttgccatcag agagtgggta
    15661 agctggtagc cggcaccctg cgggtctttc cacgggtcca aaaatgccac tacccgctgc
    15721 acacggtagg gagcggcggt aatcatcagc accatcccgc ccaagacgct gcctaccagg
    15781 acgaaaaaat atttccacgg caatcctgcc aaaaacagca ttccaacggc aatgacggta
    15841 atgacgacaa acgaaccgaa atccggctgt accattatca gcaccaaacc gaacgccacc
    15901 agcataatcg gcaggatgat cgcccggaaa cggccgtaca tttctaatgt ttcacgacgt
    15961 gcctgcggat tggtggcgga catgatcaga ttggccgtcc cccgccaaat cgactgccaa
    16021 cccaaacttt ccatgctgcg caacacttct tcacggcgcg tgaacaggct tgccaaataa
    16081 aggatgaccg ccagcttgaa cagctcggtc ggctggaaat tcaacggacc caaaggtatc
    16141 caacgggtcg cgccattgat ttcgcgcccg gcaatcaata cgactaccag caacaggccg
    16201 gatagggcaa aaatccacgg cacaagccgc cgccatgtcc tcatcctgca aagaaaccat
    16261 aacaaaccgc tcgctatcaa gctggcaacg acgaaccccg cctgtctggt caaatagaaa
    16321 aactgatcgc cgccttcttt tgatgccaaa tacacagaag ccgaataaat catcagcagg
    16381 ctgaacgccg tcatcagcac caccatccac aaaagcggcg cgtcgaattt cctgccgtcg
    16441 cgcacaatcg gcctgtcgag cagcagagtg tggacaccgt cgcccacttt taccaatact
    16501 tccgaaatct tcaaaaaaac cacctgccag tctgtttgca cctgccgcaa agggcaaaaa
    16561 tttcagacgg cagacaatgc cgtccgaaca tacgatacat cccaaatcgg tattctaaat
    16621 ctttacttgc cgcccaacaa tgacggcgtt tgcatttcag acggcatcac aaagccttaa
    16681 acgcttcgat aaacacttcc gaacggtgcg cgtagccttt aaacatatca aaactcgcac
    16741 aagccgggct gagcagcacg atatcgcacg cttcggcttg ggcatatgcc gtctgaacgg
    16801 cttcttccaa agtggcgcag tcggtcatat tcagaccgca gccgtccaaa tcgcggcgga
    16861 tttgcggcgc atcaacgccg atcaggaaca cgccttttgc cttgccggca agcgcgtcgc
    16921 gcagaggcgt gaagtcctgc cctttgccca tgccgcctaa aatcacgaag agcggatttt
    16981 gcaaaccggc aatcgcggcg gcggtcgcac cgacgttcgt gcctttgctg tcgtcgatga
    17041 acaccacgcc atttttctcg ccgatttttt ccacgcggtg cggcaggcct tgaaaggttt
    17101 tgacgtgttc gagcaatgct tcgcgcggca aaccgacggc ttcgcacaag gcaacggcag
    17161 ccatcacgtt agtggcgttg tgcaagcctt gcagcggaat atcttgcgtg gcaatcaaat
    17221 cttcattgcc ttgtttcagg cgacctgtct cacgttccaa ccaaaaatcg gcttcgtatt
    17281 ccaacgaaaa ccatttcacc tcgcgcccgg cgcgcttcat cgcacgacag aacgcatcgt
    17341 ccgcattcaa aacctgcacg ccatcgccac ggaaaatctt ggctttggta tgcgcgtagt
    17401 cgagcaagtc gtcgtagcgg tcgagatggt cttcggaaat gttcagcacc gtcgccgcag
    17461 tcgggcgcag gctttcggtg ttttccagtt ggaagctgga aagctccaac acccacacgt
    17521 ccgccttttt gccttcgcgc tgccattccg cctccaaaac cggcgtgccg atattgcccg
    17581 cgataacggt atccagcccg cacttgatac agagatagcc gaccaggctc gttaccgtgg
    17641 ttttgccgtt gctgccggta atcgcaatta ccttgtcgcc ccggcggttc acaatgtccg
    17701 ccagcaattc gatgtcgccc aacacgcgtc cgccgttttg cttgaacgcc tcgatatccg
    17761 gctgccgctc gctgatgccg ggactgagcg ccagaatatc gaaaccgttg tccagcgcat
    17821 ctttcagacg gcccgtgtaa aacaccagcc cgtcaaacat cttaccgatt tgcgacacgc
    17881 gttccggctt cagctccgca tcatacgcag caacctccgc gccgtttttg cgcaggtagg
    17941 caatcatgga aatacccgtg ccgccaagtc cggcgacgag gatttttttg ttttggaaag
    18001 tcattttggt ttgtcctaaa acaaatcata ttgggcaggg ggatgtctac tcctgctcaa
    18061 gccgctttca gacggcattg cgagctgttc aataacccgc tttcaggcgt tggtcattgt
    18121 cgcagccgtc ttggtttccg ttttgacacg ccttgccaag ccattcttgt gcaagggcgc
    18181 ggtcttggcg cacgccgtgc cctttgtaat acattgcgcc caaattaaat tgggcttgtg
    18241 ctaacccctg atccgccgcc tgccgaaacc atctgaccgc ttcggtatca tcttggcgca
    18301 cgccttgtcc ttcggcatac atcgcgccca aattgttttg ggcatcagca tctccctgct
    18361 ctgccgcctg caagttttcc cgaaaatccg atacgtcatc cgcccacacc gcttggttca
    18421 agcccaaggc aatcagggcg gcggcaagcc atttgactgt ctgtttcatg ttttctctcc
    18481 tatttcggtg tggggcggtt tttcagccac cgttaacgat aggacgggac agattgccgc
    18541 cgcaggttta ttgcgcgttc aaatgccgtc tgaaaggtgt tcagacggca taggttcagc
    18601 ggattttgag ggtactcaaa ccgatcaata ccaagacgat ggtaataatc caaaagcgga
    18661 cgacgacttg ggtttctttc cagccttttt gttcgtagtg gtgatggatg ggcgccatca
    18721 ggaagatgcg ttttttggtt ttcttatacc agccaacctg aagcataacg gatacggctt
    18781 ctacgacaaa taatccgccc ataatgacga ggacaaactc ttggcggacg atgacggcga
    18841 cggtaccgag cgcggcaccc aatgccaatg caccgacatc gcccataaag acttgcgcgg
    18901 gataggcgtt aaaccacaag aaaccgaggc acgcgccgca catggcggta cagaaaatca
    18961 ccacttcgtt tgcgccggca acgtaaggta attgcaggta ttgggcaaat tgtgagtggc
    19021 cgctggcata ggcgaagatg gcgaggccgg cggcaacgag gacgacgggg aaggtcgcaa
    19081 ggccgtccaa gccgtcggtg aggttgacgg cattggatgt gccgacgatg gtcaggtaag
    19141 acaacaccaa aaagccgacc acgcccagcg gcagggcgat ttgtttgaag aacgggacaa
    19201 tcaaaatatt gttggcggaa ttggcggcaa ggtaaaacaa tgccaaactg gcgataatgg
    19261 caacgcttga ctgccacacc attttgaatt tggcggacac gccgttgggg tctttataga
    19321 cgactttgcg ccagtcgtcg taaaaaccga gtgcgcccgt ggcgagcaat acgcccaaga
    19381 gaatccagat atacgggttt gcccagttgc cccacaacag ggtggacacg gtaatggcgg
    19441 tcagaatcag cgaaccgccc atcgtcggcg tgccgttttt gacgaggtgg gtttgcggac
    19501 cgtcggtacg cactgcctgc ccgcatttga gcgcggtcag cctgcgtatc gtccacgggc
    19561 cgaacatcag ggaaaacgcc aaggcggtca acgccgccat gacggcgcgg aatgtggtgt
    19621 attgaaaaat attcagaccg gttaaccagt tgctgaaatg tgcgagccat aaaaacatgg
    19681 ggcttccttt ttttgttttg t
//
LOCUS       DAWKQB010000026        18621 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.026, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000026 DAWKQB010000000
VERSION     DAWKQB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.026"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(83..1276)
                     /locus_tag="WJL14_000682"
     CDS             complement(83..1276)
                     /locus_tag="WJL14_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7655785.1"
                     /translation="MYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPDGTPAFTGFSG
                     TVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYET
                     SQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGS
                     YTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVD
                     FGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAA
                     EVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI
                     RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMK
                     LDWQF"
     gene            complement(1312..3171)
                     /locus_tag="WJL14_000683"
     CDS             complement(1312..3171)
                     /locus_tag="WJL14_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7655786.1"
                     /translation="MNQNHFSLKILTVMLLSAYGGSFADGVVPVSDGNTVSLDTVNVR
                     GSHALSGKTEKTRSYTIDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMK
                     NTTGVNVVRDSGLQTRFLSRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYD
                     HIEVVRGATGLTQSNSEPGGTVNLIRKRPTASFKHTGELTADHRGSRRAVLDVSGSLN
                     KANTLRGRLVGAEEYKKSFKDRVWGRKHMVYGIAEADAGDSSVLTLGGMYQKSREVPD
                     FSGIILSCENQKTAPFSSTPACNRPLQLPRNTYLGEDWSRLSADKYNLFSGFKHVFDN
                     GWQLNAEVSYTKNESDAKVGQFFLKNEHAAGLSDEDAVGFLTEKNEVIPFEPKDKALE
                     KLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYRSRRAAERKAGFDECMSAPFALDFI
                     CQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFPNSLYDSSFNRKATANRRYSYMP
                     LRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDEKIRSEYLEIYERRHRVRPNT
                     GATHGVYAGSCQGSRTVICLLLWSGGIKNPIGRRTMKKATVPFMPKNAGMPRK"
     gene            3445..4224
                     /locus_tag="WJL14_000684"
     CDS             3445..4224
                     /locus_tag="WJL14_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ7655787.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4435..5847)
                     /locus_tag="WJL14_000685"
     CDS             complement(4435..5847)
                     /locus_tag="WJL14_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7655788.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPILDWGKMHEVEITETDPLTGEKVVLHGMGGALEMGDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            5938..6014
                     /locus_tag="WJL14_000686"
     tRNA            5938..6014
                     /locus_tag="WJL14_000686"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5972..5974,aa:Arg,seq:acg)
     gene            6032..6106
                     /locus_tag="WJL14_000687"
     tRNA            6032..6106
                     /locus_tag="WJL14_000687"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6065..6067,aa:Glu,seq:ttc)
     gene            6662..7837
                     /locus_tag="WJL14_000688"
     CDS             6662..7837
                     /locus_tag="WJL14_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ7655789.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8178..8726)
                     /locus_tag="WJL14_000689"
     CDS             complement(8178..8726)
                     /locus_tag="WJL14_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ7655790.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(8729..10225)
                     /gene="ccoG"
                     /locus_tag="WJL14_000690"
     CDS             complement(8729..10225)
                     /gene="ccoG"
                     /locus_tag="WJL14_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ7655791.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLAVVIAAFLVGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINNSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10753..12732
                     /gene="tkt"
                     /locus_tag="WJL14_000691"
     CDS             10753..12732
                     /gene="tkt"
                     /locus_tag="WJL14_000691"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ7655792.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQAALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVHVVSMPSTNVFDRQDTAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            12873..14261
                     /gene="fumC"
                     /locus_tag="WJL14_000692"
     CDS             12873..14261
                     /gene="fumC"
                     /locus_tag="WJL14_000692"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ7655793.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14326..15249)
                     /locus_tag="WJL14_000693"
     CDS             complement(14326..15249)
                     /locus_tag="WJL14_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7655794.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAELAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALVVVGGAVTAAVGDRLFKRR"
     gene            15421..15561
                     /locus_tag="WJL14_000694"
     CDS             15421..15561
                     /locus_tag="WJL14_000694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655795.1"
                     /translation="MPSENSGDVRQASLPQNARTAQSQRSAQRKGRQQAGSDAKKRNP
                     AP"
     gene            complement(15621..16145)
                     /locus_tag="WJL14_000695"
     CDS             complement(15621..16145)
                     /locus_tag="WJL14_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ7655796.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
     gene            complement(16149..17531)
                     /locus_tag="WJL14_000696"
     CDS             complement(16149..17531)
                     /locus_tag="WJL14_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7655797.1"
                     /translation="MARDNRIQMFPHEWRASTMLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEVLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            complement(17642..18265)
                     /locus_tag="WJL14_000697"
     CDS             complement(17642..18265)
                     /locus_tag="WJL14_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7655798.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAQAGAQREETLADDV
                     ASVMRSSVGSINPPRLVFDNPKEGERWLSAMSARLARFVPDEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
BASE COUNT         4631 a         5366 c         4398 g         4226 t
ORIGIN      
        1 gccccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacggtttcc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttagcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaaatca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt ataatggtcc tgatccgcct
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaagatgc agtcggccgg
     1021 ttacgttgaa acgcgtgccg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatggtcgt cggccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg tgccgtcggg attggtctcc tgaatcttat
     1201 tgcctttttc atccagttca tatacatcga catatactgg cgtccggctg ccgctgtatt
     1261 cgtcatagta atacacctgc ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcattcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgcctgcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagaagacaa atcaccgtcc ggctcccctg
     1441 acaacttccc gcatacacgc cgtgcgttgc ccctgtattc ggacgtactc tgtggcggcg
     1501 ttcgtagatt tccagatatt cggaacgtat cttttcatcg ccgtaggcat agccgacaaa
     1561 gaaatcatgc tcccgcccga acagcccata tgtgccggtc aggtcaagtt taattcccca
     1621 ttggcggtcg tctttggtat gccgcaacgg catataactg tatcgtcggt tggcggtagc
     1681 cttccgatta aaggaagagt catacaggct gtttggaaaa cgttgtgccg cattattaaa
     1741 gatgccttcc ttcgcaaggg ctttatcgac aaattccgac ttgtcggcat caacacccgg
     1801 atccccccaa gaaccttgac agataaagtc cagcgcaaaa ggggcactca tacactcgtc
     1861 aaaaccggct ttgcgttctg cggcacggcg gctgcggtac tgctcgaaag cagtattatc
     1921 gaaacggttt ttaacaaaat cgtctttgcg ctcacggtat tccttggcgg tttcgtcacg
     1981 atatgctttc agtttctcca atgccttatc tttcggctcg aacgggatga cttcgttttt
     2041 ttcggtcaaa aagcctaccg catcctcatc tgacaaaccc gccgcatgtt cgtttttcag
     2101 aaaaaactgc cccaccttcg catcggattc attcttggta taagacactt cggcattgag
     2161 ctgccaaccg ttgtcaaaca catgtttgaa acctgagaaa aggttgtatt tgtcagcact
     2221 caaccgcgac caatcctccc ccaaataagt gttgcgcggc agttgcaaag gccggttgca
     2281 ggcaggcgtt gaactgaacg gggcagtttt ctgattttca caggacaaaa taatgcccga
     2341 aaaatcagga acctccctac tcttctgata catgccgccc aaagtaagca cactgctgtc
     2401 gcccgcatcg gcttcggcaa tgccgtaaac catatgtttc ctgccccaaa cgcggtcttt
     2461 aaacgatttt ttatactctt ccgcacccac caaccttccg cgtaaggtat tcgccttatt
     2521 caggctgcct gaaacatcca acactgcacg ccggctgccg cgatggtcgg cggtcagctc
     2581 tccggtatgt ttgaaagaag cggtaggtcg cttacggatc aaattgacgg ttccacccgg
     2641 ctctgaattg gattgggtca accccgttgc accccgtaca acttcaatat ggtcataaac
     2701 cgccaaatcg gtactcggag acacgtcgat tttcgccgta tatcccgaac ggcctgcaac
     2761 attgacggta ataccgtctt caccaatctg atcaatatag aaaccgcgtg acaaaaaccg
     2821 cgtctgcaag cctgaatcgc gcacaacgtt gacacccgtc gtgtttttca ttgcctcttc
     2881 aagcgtatgc accgccttat cgtcaaggcg gctgcgcgtg atgacgctga ccgactgcgg
     2941 cgtatccttg cccgcaatcc tcatacctgt ggcggtggac atccgatcta tcgtataaga
     3001 acgggtcttc tcggtcttgc ccgacagagc atgagagccg cgtacattga ccgtatccaa
     3061 actgacggta ttgccgtctg aaacaggcac aacaccgtct gcaaaagaac caccgtaagc
     3121 cgataacagc ataacggtca gaattttaag tgaaaaatga ttttgattca tagagacctc
     3181 tgtaatatgc aagtgtgcaa atcgtccaaa ggccctcaca actgttttga ttttttatat
     3241 taattgaaaa aaagtaattc tcaattaaat ttatagatgg gattgtattc ccattttgac
     3301 aaaaaacaaa ctgctcctta ccgtttattt caaaaaacga taatattgta ttgaaaaata
     3361 tccgaattta aatacagatc gtcaatgcgg aaaaaaacac ccaaattggc tataatcccg
     3421 acaaacacac tcaaggacaa caacatggca gcctcgcccg aagcaaaatt cactgaagaa
     3481 aagattttgt gggtcaaaca ccacacgccg aaactcatca ctttcgccat cagccgtccc
     3541 gaatcctacc gttttaaagc cggacagttc tcccgactcg gtttctacga aggggaaggc
     3601 tttatttggc gtgcctactc catcgtttcc gcagaatatg cagacacgct cgaatatttt
     3661 gccgtactca tccaagacgg ccccatgtcg gcccgtttcg ccaaaatgca acagggcgac
     3721 accatcctgc tcgataaaaa tgccaccggc ttcctcctgc ccgaacgctt ccccgacggc
     3781 aaggatttgg tgatgctctg caccggctcc ggcatcgccc ccttcctttc cattctcgaa
     3841 caacctgaaa tccgacagcg tttcgatacc gtcaacctga tacattccgt atcttttccc
     3901 gaagaattga ttttcaacga ccggctcgcc gcattgtccg aacatcccct ggtaggcgaa
     3961 tacagacact cgttccattt cgtccctgtt accacccgtg ccgccaaccc ctcgggctta
     4021 agcggaaaac gcattccgga actcttaaaa aacagcagca tcgaacaggc gctgcatacc
     4081 aagttcaccc cggaatccac acggtttatg atttgcggca acccggaaat ggtcaaagat
     4141 actttccaaa cgctgcttga catgggttac gccatgcacc gcaaccgcat tcccggtcaa
     4201 atcatgatgg aaaacggctt ctaaaaacca ccctgcttgt ccgatgcctt cggatggacg
     4261 gtcaaaccga cacggcacga aaaccgcgtc ggcaaaaatg ccgtctgaaa aaattcagac
     4321 ggcatcttcg gacacattac ctgcaaacgg caacacattc caacccaaac cgattaggca
     4381 atcaatacga acggctgttt acatacttac cggctttcat caaccggtat cgatttaacc
     4441 gatttcctta atatttttcc tgtccgtttt aaacttcgcc ttaaacgcat ccggtaaatc
     4501 tttatcgaaa taccaaagcc cgtcacccat ttccaatgcg ccgcccattc catgcagaac
     4561 aactttttcc cccgttaaag gatcagtttc ggtaatttct acctcatgca tttttcccca
     4621 atccaaaata ggcaacttgt gtgcaaacgc caaaacctgt tcgtcagaac ggccgcacgc
     4681 cttatcgcat cggcctgaaa catatacggg acaaggctca tcctctgcat aaccgtccgg
     4741 aaggataccg gctgaggcag gacacaatcc gtatgtttcc gcccacgacg acaaagcccg
     4801 tctcgccgcc tttttattgg caaataatcc ggtaggcgga ttatccgtca caccgttttt
     4861 caaagccgct gttttcgcat tcaacatgcc gtctgaacct ttttcaaacc tgacggtcgt
     4921 aaatgtttta agcagatttt tggcagatac ataacaaccc gaatgataac gtccgaccaa
     4981 ttccgcttta atcttatatg catgcaggct gcccaatgca ggaaaaaaac ggacttcctc
     5041 cgtattgcac caatcaaacg gggcttttcc ggaatccaat aaagccgaaa cctcgctata
     5101 tacccgttca aacgtaccga tgtaatttac tttccctccg ccgtcgaaac aagccagcac
     5161 ccccataccg tcaggcaaac cgtacaactg ttccctcaac cgttcgggca gcgcggcagg
     5221 cagcggtttc ggattcatca aacggaaaca ctgcctgatc catgcctcaa ccccatgttc
     5281 cgacaaactg tattccaaat aatcacacaa tgccgacaca tccgccatcg cacgatgcct
     5341 gtcttctaca acaatcccca acctttcgat gatactgtcc aggctgtgct tgtaaaattg
     5401 cggatacaga caccgggaca gttgcacact gcacaaagca ggcgatgaaa acccgatgcc
     5461 cgcacgatga aactcatgct ttaaaaacgt atagtcgaaa cggctgttat gtgcaaccag
     5521 cacacaaccc ttcaataccg aaaacaactc gccggcaatc tctgcaaaaa caggcgcatc
     5581 ggcaaccatg ccgtctgaaa tccccgtcag ccccgccaca aactgcggaa tcggtttttg
     5641 aggattaacc aaccactcat gcctcaccac ccttccctgc tcaaacttga ccaaagccac
     5701 ttcggttacc ctgtcttcat acagattgcc gcccgtcgat tccaaatcga ccacggcaac
     5761 aggcattcca aaccgtaaaa ataccttttc cagcaagggc cagcgggaag caacaatcat
     5821 tttattctct ttaaattcaa acaacaaacc gatattttac acttttaagg catttcatcc
     5881 aacaaaacaa ttgacagaat ccgatgatta ccctaaaatt caaatctttc ttgcagtgca
     5941 cccgtagctc agttggatag agtatctggc tacgaaccag agggtcgggc gttcgaatcg
     6001 ctccgggtgc gccagtaaga aaatacaata tgcgcccatc gtctagcggt taggacatcg
     6061 ccctttcacg gcggtaaccg gggttcgatt ccccgtgggc gtgccaaatt cccaatccct
     6121 gagattatcc ctcggggatt tcttattacc tcgacaactc gttaccatat ctttaccccc
     6181 cttcatcaga ctctcagacg taatcgaatc atattcaaac ctttgccgtg caaaccgata
     6241 tcacgtaacc ggatgcggtg tccgtccaac attttacccg attgaaacgc ctgatatatt
     6301 gcaccccatc aacgtggcat tacttttctt aacaatcccc tttgacagca actgactagg
     6361 gcttttttat gccatcatca aatttatagt ggattaactt taaaccagta cggcgttgcc
     6421 tcgcctttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta
     6481 attcactata atattttctc tcccgattga aacaggcgta acagaatatg cccgaagctc
     6541 cggctgcttt cttgtttacc gccgcgatat ttagagtata ataccaaatt tgagcaatag
     6601 ttctaaaaca gttagaacca tttttcatga gcctgactga ttcgtacact cggagaaact
     6661 gatgcagaat atttttgacc ctttggttat tcgtggaaaa tcccttaccc ccatcgtgca
     6721 aggcggtatg ggggtcggtg tttccgcatc gggtttatcc agcgcggtgg cacgtgaaaa
     6781 cggtatcgga acgattgcca gtgtggattt gcgccacctt cacgaagacc tactcgccga
     6841 atcacaaatc aatccgagtg aagagaaata tacatctttg aactgtaccg cattagacag
     6901 ggaaatccaa aaagccaaaa gcgcttcaga gggaaaagga ctgattgcgg tcaacgtgat
     6961 gaaggcggtc aaagaccacg ccgcgtatgt ccgccaggct tgcgaatcag gggcggatgc
     7021 ggttgtaatg ggtgccggtc tgcctttaga cctgccggaa atgaccgagg gctatcataa
     7081 agatgtcgcg ctgctgccga ttctgtccga atcgcgcggt attaatatcg tcttgaaacg
     7141 ttggatgaaa aaaggcatat tgcccgatgc gattgtagtc gaacatcctg cccacgcggc
     7201 cggacatttg ggtgcatcaa ccgttgaagg cgtaaacgat gccaagttcg acttcaaacg
     7261 cgtgattgag gaaacgtttg aagttttcaa aagtttaggg ctggaaagcg aaaaaatccc
     7321 gcttattctt gcgggaggca tggcaaattt tgaaaaagtc aaaaccgccc taaagaactg
     7381 gggagcatcc gccgttcaaa tcggtacggc ttttgccgtt accgaagaag gagatgcaca
     7441 ccttaacttc aaaaaaacgc tcgccggtgc ggaaactgaa aaagtagtcg aatttatgtc
     7501 tgttgccggt ttgccggcgc gcggtgtccg caccaaattc ctagacagct acatcaagcg
     7561 tgaaagcaaa cttcagacaa acgccaaagc cgacccgcgc cgctgtaccc aaggtttaaa
     7621 ctgcctaacc agttgcggtc tgcgcgacgg gctttccaaa gccggacaat tctgcattga
     7681 tatccagctt gccgccgcat tccgtggaga agtagataaa ggcctgttct tcagaggtaa
     7741 agacccgctg cccttcggca atgccatccg caccgtccgc gagacgatac aatatctgct
     7801 gacggggagc gaacctgttg caacgctcgg acgctgacat caggtaggat ttgatttgca
     7861 aacaaaatgc cgtctgaagc ctttcagacg gcatttttca ggctgcgttt cggaatagtg
     7921 tcgaaaaaac ggagagaaat aaaaacgttt ttatgtaaac aaaacgcctt ccgcattgtg
     7981 tcgggcatac aacacaaatg ccgtctgaag cccttcagac ggcatttttc aggctgcatt
     8041 tcggaatggt gttaaaaaat aaacgggata agatacattt attttcgtcc gataaatcag
     8101 accatcacgc cgaataatca aacaatgcct gcccgacatt gcatctggca aaccaatgca
     8161 atgaaaacac ggctttttta tttgcttccg gcattattga aaagtttatc catcggggtc
     8221 agatccaccg cattgccttg gctggtaatc catttgttct cgacgcgcca cacgcctgcc
     8281 gcgtcctcca cgcgcacata ccagtggttg gtcggcgaaa gggttttgaa caccgcctca
     8341 tattccgccc tgccgttctg cgcgctgccg acgggcttga gggcgacggt ttgatcgtcc
     8401 gccttgcggg tcgggtgcat cagcagcagg ttcaaaggct gtttgccgtc aaactcgccg
     8461 ccgacaaaca cttttgccgc attcatatcg ggggaaatga gaacctgcac cccgatatgc
     8521 cgtctgacgg cttcttcatc ccgatgaagc tggatgtcga tatgtttgcc gtctttataa
     8581 taatcgtccg taaccaaatc tgtcgcgtgc tgctgcgcga caaaaaacat agcgacgctg
     8641 gcgatgacga caaaaatcgg ccccgccatc aagatccacg gccagacgtg tttgtaccaa
     8701 ggtttgattg gagtgttttg agacacggtt attctccgat aaaggttgca tcttcttcca
     8761 agacgaccgg cttgccgtcg ggcgcgccgc tttcgcggta ttggaaggta aattcgatag
     8821 ggtggctgcc tttgtccgcg tattccggaa tggtggacac ttggacggga agggttaccg
     8881 tttcgcgcgg ggcaaccttg ataccgcctt cgggcagccc ggtcagggcg atttcgtcaa
     8941 agcctttgac acttgcggta atcagctgtt ctttttcgct gttgttgatg atacgcaggc
     9001 tgtatgcgtt ttccagccag cctttggcgt tttcgcgcac cagtacgcca cggtctttca
     9061 aaatatcgac ttcgaccatt ttgcgcgtgg acaaaccgac caaaaaggcg gcgataacca
     9121 ctgccaacac cgcgccgtaa cctgccacgc gcggtctgag cagccgtttt ttaatgtctt
     9181 tttcggaata ttcgtgttcc aacgcgcttt cggtcgtata acggattaat ccgcgcggat
     9241 agcccatttt gtccataatc tcatcgcacg cgtcgataca ggcggcgcag ccgatacatt
     9301 ggtattgcag accgttgcgg atgtcgatgc cgacggggca gacttggacg cacatcgcac
     9361 agttgatgca gtcgcccaaa cccgcctctt ccttattgac cgttttcttg cgcgcgccgc
     9421 gcggttcgcc gcgttccgcg tcataagaaa caatcagcgt gtccttgtcg aacatcgcgc
     9481 tttggaaacg tgcatacgga cacatatgca ggcatacttt ttcgcgcata atgtgggcga
     9541 agaagaaggt cataaagccg taaaacgctg tggcaaacat cgcgccgccg cctgctgctc
     9601 cagtgaataa atcgggaacg aactggcgga tagggacaaa ccagcctgca aacgtgatgc
     9661 ccgtccacgc gcagacaagg aaaatcagca ggtatttggt ggctttgatg cggattttag
     9721 tgaaattcca cggcgatttt tccagtttca gccgtttgtt tctatcgcct tcgaccaggt
     9781 tgtcgatcca cagcataatt tcggtgtaaa ccgtttgcgg gcaggaatag ccgcaccaca
     9841 gtcgcccagc aatcgtcgtc caccaaaaca gcccgaaggc gcaaatcatc agcagcaagg
     9901 caaggtaaat caaatcgccc acccccaacg acaatccgaa aatgaagaaa tgccgttcgg
     9961 ggatattgaa aacgacggcc tgcctgccgc tccagttgaa ccacggaatg acgtaaaaca
    10021 caaactgcgt cgccaatacg gcggcgatac ggagtttggc gaaccgtcct tccgcctttt
    10081 tcggatggat gcgttcgcct tcgggatgga tttgaatcac gctggctcgc ggatcgaatg
    10141 ttttttttgc tttcggcgcg gcttttgttt gttcggacgt gccgattccg gatgccggac
    10201 tgccggcttg gttttccgtg gtcattctgc attccttaga tttttgattg atggtttgcc
    10261 cgttaccgcc gccgtttgct tttcagacgt catttttctt gttttttaag gcgttgtgtt
    10321 tcaagttttg agaaaatccg tttttcccaa aatatatttc cgctattgta caactttatg
    10381 cgccgtccgg atgtatgggg cggatacatt tcccatccgc atcaaaacgc ctggatttta
    10441 ccttaccgcc cgaacaaaat ccgaatacgg ttaaaaaaaa agactaaaaa aaccgacacc
    10501 cccatatcgg cagaaccgac ggtgcaagct cataaacaaa cgctatcgac aatccggcac
    10561 acaatctata actttttatt tcaaaaggaa taatggcagg cttcgcccgc aaatcgaaaa
    10621 tccttcccca ccggtctcct gccgccgcct tcccccgcgt ccgccctttt cttgaaagca
    10681 taagcgaatc gggcgataat caacgctttc cgattatcca cttatccgaa acaccagcaa
    10741 ggaaaataca aaatgtctca actggcaaac gcaatccgct tcctctcggc cgatgccgtt
    10801 caaaaagcca attccggcca ccccggcgcg cctatgggta tggcggaaat ggcggaaaca
    10861 ttgtggacga aattcctcaa tcacaatccc gccaacccca aattctacaa ccgcgaccgc
    10921 ttcgtcctct ccaacggcca cgcgtctatg ctgttgtaca gcctgctgca cctgaccggc
    10981 tacaatctga gcattgaaga cttgaaaaac ttccgccaac tgcacagcaa aacccccggc
    11041 catcccgaat acggctatac cgacggcgtg gaaaccacga ccggcccgtt ggggcaaggg
    11101 attgccaacg cggtgggcat ggcattagcc gaaaaaatcc ttgccgccga atttaataaa
    11161 gatggtttga acatcgtcga tcattacacc tacgtcttca tgggcgacgg ctgtctgatg
    11221 gaaggcgtat cgcacgaagc ctgttcgctc gccggcacct tgggcttggg caaactgatt
    11281 gttttgtatg atgacaacaa tatttccatt gatggtaaag tggacggctg gtttaccgaa
    11341 aacatcccgc aacgctttga aagctacggc tggcacgtcg ttcccaatgt aaacggtcat
    11401 gacaccgccg ccattcaagc cgccatcgaa gccgcacgtg ccgaaaccgg caaaccgtcc
    11461 atcatctgct gcaaaacctt aatcggcaaa ggcagtgcca acaaagaagg cagccacaaa
    11521 acccacggcg cacctttggg cgcggacgaa atcgaagcca cgcgcaaaca tttgggctgg
    11581 acttaccccg cctttgaaat cccgcaagaa atttacgatg cgtggaatgc caaagaacaa
    11641 ggcgcgaaac tggaagccga ctggaacgaa ctgttcgcgc aatatcaagc caaatatcct
    11701 gccgaagccg cagaatttgt gcgccgtatg gataaaaagc tgccggacaa tttcgatgaa
    11761 tacgttcaag ccgcattgaa agaagtgtgc gccaaagccg aaaccatcgc cacccgcaaa
    11821 gccagccaaa acagcatcga aatcttggca aaagagctgc ccgaactggt gggcggctct
    11881 gccgacctga ccccgtccaa cctgaccgac tggtcaaaca gcgtttccgt tacccgcgac
    11941 aaaggcggca actacatcca ctacggcgtg cgcgagttcg gtatgggcgc gattatgaac
    12001 ggtttggtat tgcacggcgg cgtaaaaccc ttcggcgcga ctttcctgat gttcagcgaa
    12061 tacgagcgca acgccctgcg tatggctgcg ttgatgaaaa tcaaccctgt atttgtgttc
    12121 acccacgatt ccatcggttt gggcgaagac ggcccgaccc accagccgat tgagcaaacc
    12181 gccaccctgc gcctgattcc gaatatggac gtatggcggc cgtgcgacac cgccgaatcc
    12241 ttggtcgctt gggcagaagc cgtcaaagcc gccgatcacc cgtcctgcct gattttcagc
    12301 cgtcaaaacc tgaaattcca agcgcgcagc gagcaacaac tgaacgacat caaacgcggc
    12361 ggctacgtca tcagcgaagc acaaggcaac gcccaagccg tcatcattgc caccggttcc
    12421 gaagtcgagc tggctttgga agcgcaaaaa gccctcgccg cgcaaaacat cgccgtgcac
    12481 gtcgtttcca tgccgtccac caacgtattc gaccgccaag acaccgccta tcaagccgcc
    12541 gtcctgcccg aaagcctgcc gcgcatcgcc gtagaagccg gacacgccga cggctggtac
    12601 aaatacgtcg gactgaacgg cgcagtcgtc ggcatcaacc gcttcggcga atccgcccct
    12661 gccgatttac tcttcaaagc attcggcttt accgtggaca atgtggtcga tacagtgaaa
    12721 tcagtgctgt aaccccactc ctaaacaaat gccgtctgaa cgcttttcag acggcatttt
    12781 tttgccctcc cgccttgatg cttcctcatt ccaccaatct ccattataat atttgcgaat
    12841 cactcttatt cacatttcaa aaggagaaac gtatgaacac tcgcaccgaa cacgacacca
    12901 tgggcaatgt cgaagtcccg tccgaagcct attggggcgc gcagacccag cgcagccgca
    12961 acaatttcaa aatcggcggc gaaaccctgc cgcagccgct gatttatgct ttggcgttgg
    13021 tgaaaaaagc cgctgccgcg accaatgttt ccctcggcag gattaagccg gaacaggcgg
    13081 atttgattac gcaggcggcg gatgatgtgt tgaacggcaa gctcgacgga cagtttcctt
    13141 tagtggtttg gcagaccggc tccggcacgc agtccaatat gaacatgaac gaagtgctgg
    13201 cgaaccgcgc caacgaaatc gccggtacgg gtttggcggc gtatcagccc gtccatccca
    13261 acgaccatgt gaaccacgcg caatcgacca acgacgcatt cccgaccgct atccacgttg
    13321 ccgccgcgat tgaaatcaac cgccacctca tccccgccgt aaaagccctg cgcgacacct
    13381 tggacaaaaa agcccaagcc ttcgccccta tcgtcaaaat cggccgcacc cacttgcagg
    13441 acgcgacgcc gctgacttta ggccaagaat tttccggcta cgtttcccag ctcgaccacg
    13501 gcttaggccg tctgaacgac gcgcttaaag acttgtatga acttgctttg ggcggtacgg
    13561 cggtcggcac gggtttgaac agccatcccg aatacgccga aaaagccgcc gccaaactcg
    13621 ccgaattgtc cggcttgccg tttgtcagcg cgccgaacaa atttgaagcc ttgggcggac
    13681 gcgatgccgc cgttgccgct tcgggcgcat tgaaaacgct ggcggcaagc ctgaataaaa
    13741 tcgccaacga catccgctgg ctggcaagcg gcccgcgctg cggtttgggc gaaatcaaaa
    13801 tccccgaaaa cgagccgggt tcgtccatca tgccgggcaa agtcaacccg acccaatgcg
    13861 aagcgatgac catggtgtgc tgccaagtgt tcggcaacga cgttaccatc ggtatggcgg
    13921 gcgcgtcggg caatttcgag ctgaacgtct atatgcccgt catcgcctac aacctcttgc
    13981 aatccatccg cctgttgggc gacgcgtgca acagcttcaa cgaacactgc gccgtcggca
    14041 ttgaacccgt accggaaaaa atcgactatt tcctgcacca ttccctgatg ctcgttaccg
    14101 cgttaaaccg caaaatcggt tacgaaaacg ccgccaaagt cgccaaaacc gcctacaaaa
    14161 acaacaaatc gttgcgcgaa accgccgttg agttgggctt gctgacgggc gaagaatttg
    14221 acgaactggt cgttcctgcc gatatggttc atccgcgcta atctttccct caaataaaat
    14281 gccgtctgaa acctcgttcg gacggcattt tccgttgcct gcgaactagc ggcgtttgaa
    14341 cagcctgtcc cccaccgccg ccgtaaccgc acccccgacc acgaccagtg cgccggcata
    14401 acccaaaccg ttcatatccg gcgcggcaaa agtatcaggc atcacataat gcccgagcaa
    14461 agaaaatatt acggtaaaca cggggagcaa ggttgttacc gcgctgactt tggaagcctc
    14521 ccaatgtttc aacgcctcgc cgaacgagcc gtaaccgatt aacgtattca agcagcaata
    14581 cgcaaaacaa acccacgcca acgtaccgtc caaacttccg atgtgtgcca gttcggcaaa
    14641 cggcaggaac acggcggcac ttgccgcata aatcaacagc agaatctgtt gcggcccgaa
    14701 ttgcgccgac agcagctttt gcgccacggc ataacacacc catgccatac tgcctgccgc
    14761 acacagcaac acgcccttcg catacgcgcc caaacccgac aactcgccga atttatcgtt
    14821 aaaaaacata agcaaaccgg caagcagcaa aaccaagccg attttctgag cggcagtcat
    14881 ccggtcttta aacaccaaca caccgacaac aatcatcgta aacggcgaaa tctgccacaa
    14941 aacctgcgtc gtggtcggcg aaatataatg cagcccttgg gcaatcagca caaagtttgc
    15001 cgaaatgccc gccacgccga gcagcagcag cctgaatgag caccaagaaa aatcccgcca
    15061 cttcggcagc cgcccgccca atgccagcaa aacaaacaat accgccgccg ccacggtaaa
    15121 acgcacccac accagcgtcg gcgcatcgac aaacttcaat acctgccgca cggcaatcgg
    15181 cagcgttccc cacgtcatcg ccgccaaaag tgccaacgcg aagcctagga gcggcctttg
    15241 gttttccatc ctgattttcc tatttttaaa caaccgtatt gccggacgat gccggtttgc
    15301 cgcatcgggc aatgatggtt caagcgtttg acgtttgatt ccaacccttt gatttcaaac
    15361 aaaccggctg aagcccgact attgcttcgc gctatttgga aacaccgcct gaattttaaa
    15421 atgccgtctg aaaacagcgg ggacgtgcgg caggcatcac tgcctcaaaa cgcccgaacg
    15481 gcgcaatcgc aacgttccgc ccaacgcaag gggcggcaac aagccggctc ggatgcaaaa
    15541 aagagaaacc ctgccccgta aggtttaagg tttctccgtc ctttatgatt tccctctgcg
    15601 aggatgtccg gccgtaaaat tcagaacggg atgtcgtcgt caatatcctc gaccggggcg
    15661 gcggcaggca cgggttggcg gcgcggcgcg gctggtgctt cttggggatg ggacggcgcg
    15721 tcggaggcgg gctgccggct ttgctgtgcg gggcgttggt aagcctcctg actctgaccg
    15781 taaccttcct cgtaaggcgc accgccgctg ttttcattgc gcccgcccaa cattttcatt
    15841 tcgttggcga caatatcgta agcggtgcgt tcgatgccgt ctttgccttg gtatttgcgg
    15901 ctttggattc tgccttccag ataaaccagc ccgccttttt tgaggtattg cccggcaatt
    15961 tccgccagtt tgcggtacat cgtgatgttg tgccactcag tacgctctac acgttggccg
    16021 ttgcggtcgt tccaagtttc gctggtggcg acgctgaaat tacacaccgc ctcgccgttg
    16081 ggcatatagc gcacttcggg atcgcgtccg aggcggccga taaggataac tttgttcaat
    16141 gacatttttt aaactcctgt gatgattttt tcagcggcag cctgatcgaa acccttctgc
    16201 aacactttga gatagacggt ctgcccgtcg aaactgaaac cgatgtcttc cacaccctca
    16261 agctccgaca aggcgcggta taacacttcc tgattgacct gccacacgcc gccgacaggg
    16321 taactgaggt ttttgacggg cttgggcgca ggcgataaaa cggcaatcac cagccacagc
    16381 agcatcaata tgctgcaaaa ggcaaacacg ccggaaaagc cgtatttttg aaacagcaaa
    16441 ccgcctgcca cgccgccggc aaacagtccg agcgactgca tcgtgttgta cacgcccatc
    16501 gccgtaccct tcaggtcgga cggcgcgatt ttggaaacca tagacggcag gctcgcttcc
    16561 aacacattaa aaccgataaa gtaaacaacc aaataagcgg taatcaagcc taccgagcgc
    16621 ataccggaca gcaaaccgag ctgtgccgcc gcgatgcagg cgatgcccaa gataaaaacc
    16681 tgtttgagct tgttgcgcgt ctcgccgacg ataatcagcg gaaccatcac caccaagccc
    16741 gtaatggtcg agggcagata gactttccaa tgctggattt tttccaaacc gagctgcgtc
    16801 atcgcaaaag gcagcgcggt aaacaatgcc atttgcgcgg cgtgcagggc gaaaatgccg
    16861 aaatcgagcg tcagcagcct acggtttttc aaaacttcgc ctatgcgcga aggctgcgcc
    16921 tgcgtatctt cgtgcagctt ggaaacctcc gggtcgggag tcatccacgc caccacgccg
    16981 atactggcaa cggtcaaaat accggtcagc agaaacaagc cgcgtacgcc caccgcgtcg
    17041 gcaatcacag gggcaacgac gaggctgacc gaaaacgtca gaccgatact caaaccgatc
    17101 atcgccatcg cgcgggtacg tacgccgtcg cgcgtcaaat ccgccagcag cgcggtaacc
    17161 gccgcactga ccgctcccgc accctgtatg gcgcgtgcgg cgaccagcat gggcagcgta
    17221 tcggcggcgg cggcaaggaa gctgcccgct gcaaacacaa tcagcccgac ataaatggtt
    17281 ttcttgcgcc cgaacttgtc ggaagcgatg cccaaaggca gttgcagcag agcctgcgtc
    17341 agcccgtaaa tgcccattgc cagcccgacc agcgttttgt tgccttccgc gccgggcagc
    17401 gaggcggcat acaccgccaa tacgggcagc acgaggaaca tacccagcat acgcagcgcg
    17461 tacacgccgg aaagcatcgt actggcgcgc cattcgtgcg gaaacatttg gatgcggtta
    17521 tctcttgcca tcatattttt tcagacggca tcaacagttg caatgccgtc tgaacttcca
    17581 gtgaacagat tttcggatta tacaggattc gccgtatttc ggttgcggcg cgggttcaaa
    17641 atcaacgcca ctgccagcgg ttgcgccacg cgcccaaaac ggcgttcgga tatttattgc
    17701 tgccgaggct accgttaaaa cgggcgagtg cgcggacgat gttgcctttt tcaagattcc
    17761 ggtaatggcg caggatggta cagccgtaac gcaggttggt gcggatgtcg aacaggttgt
    17821 gcgccggttt gccgatgtag tttttccaaa acggcataac ctgcatcagg ccgcgcgcgc
    17881 cgacaccgct gattgcatac tggcggaacg cgctttccac ctcaatcagc cccaacacaa
    17941 tctgcgtatc caaaccggcc cggctgcttt cgtactggat attgaccagc agcctgcgcc
    18001 gctcctcctc atcggggacg aaccttgcca accgagcaga catcgcggac agccaacgct
    18061 cgccctcttt cggattgtcg aacaccagcc tcggcggatt tatgctgccg acagagctcc
    18121 tcatcacgga agccacatcg tcggcaagcg tttcttcacg ttgcgcgccg gcttgtgcca
    18181 gcgggctgag caacagcgcg ccggcagcac acaataggcg gcggcgttgc agattgacgg
    18241 gtagggtatc ggtcggtttt ttcataggga acgggggcgc gtccggacgt ttcagacggc
    18301 attaaatatt caaacagaga tgattgcttc caacgcgaaa aaccgcgcac aaaatccaag
    18361 cacggcatat cgccctgccc ttttcgggca aactccgatt ttaccgctct caagaacgct
    18421 tgtccaaaca ggcacagaca acaccgcccg gacatttccc gttttcaccg gctatccgtc
    18481 gtccggatta tgcagcagca ccatcagcgc atcacgcttt tcgggcggca gcaggcggaa
    18541 atatagtaga ttaaatttaa accagtacag cgttgcctcg tcttaccgta ctaatttaaa
    18601 tttaatccac tatatcttga g
//
LOCUS       DAWKQB010000027        18447 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.027, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000027 DAWKQB010000000
VERSION     DAWKQB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.027"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     repeat_region   378..1798
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=444..479
                     /rpt_unit_seq="gttgtagctccctttctcatttcgcagtgctacaat"
     gene            complement(1862..2188)
                     /gene="cas2"
                     /locus_tag="WJL14_000698"
     CDS             complement(1862..2188)
                     /gene="cas2"
                     /locus_tag="WJL14_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ7655799.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(2181..3095)
                     /gene="cas1"
                     /locus_tag="WJL14_000699"
     CDS             complement(2181..3095)
                     /gene="cas1"
                     /locus_tag="WJL14_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEZ7655800.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QLWQHIVRQKILNQAFVADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARALTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKTELTLGIKQHLIKILYYQTSIERQHFSTLAAIDKMIS
                     SFQAGVTDKNAKQLKLPEILPLKEYQYE"
     gene            complement(3162..6410)
                     /gene="cas9"
                     /locus_tag="WJL14_000700"
     CDS             complement(3162..6410)
                     /gene="cas9"
                     /locus_tag="WJL14_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEZ7655801.1"
                     /translation="MAAFKPNPINYILGLDIGIASVGWAMVEIDEDENPICLIDLGVR
                     VFERAEVPKTGDSLAMARRLARSVRRLTRRRAHRLLRARRLLKREGVLQAADFDENGL
                     IKSLPNTPWQLRAAALDRKLTPLEWSAVLLHLIKHRGYLSQRKNEGETADKELGALLK
                     GVADNAHALQTGDFRTPAELALNKFEKESGHIRNQRGDYSHTFSRKDLQAELILLFEK
                     QKEFGNPHVSGGLKEGIETLLMTQRPALSGDAVQKMLGHCTFEPAEPKAAKNTYTAER
                     FIWLTKLNNLRILEQGSERPLTDTERATLMDEPYRKSKLTYAQARKLLGLEDTAFFKG
                     LRYGKDNAEASTLMEMKAYHAISRALEKEGLKDKKSPLNLSPELQDEIGTAFSLFKTD
                     EDITGRLKDRIQPEILEALLKHISFDKFVQISLKALRRIVPLMEQGKRYDEACAEIYG
                     DHYGKKNTEEKIYLPPIPADEIRNPVVLRALSQARKVINGVVRRYGSPARIHIETARE
                     VGKSFKDRKEIEKRQEENRKDREKAAAKFREYFPNFVGEPKSKDILKLRLYEQQHGKC
                     LYSGKEINLGRLNEKGYVEIDHALPFSRTWDDSFNNKVLVLGSENQNKGNQTPYEYFN
                     GKDNSREWQEFKARVETSRFPRSKKQRILLQKFDEDGFKERNLNDTRYVNRFLCQFVA
                     DRMRLTGKGKKRVFASNGQITNLLRGFWGLRKVRAENDRHHALDAVVVACSTVAMQQK
                     ITRFVRYKEMNAFDGKTIDKETGEVLHQKTHFPQPWEFFAQEVMIRVFGKPDGKPEFE
                     EADTPEKLRTLLAEKLSSRPEAVHEYVTPLFVSRAPNRKMSGQGHMETVKSAKRLDEG
                     VSVLRVPLTQLKLKDLEKMVNREREPKLYEALKARLEAHKDDPAKAFAEPFYKYDKAG
                     NRTQQVKAVRVEQVQKTGVWVRNHNGIADNATMVRVDVFEKGDKYYLVPIYSWQVAKG
                     ILPDRAVVQGKDEEDWQLIDDSFNFKFSLHPNDLVEVITKKARMFGYFASCHRGTGNI
                     NIRIHDLDHKIGKNGILEGIGVKTALSFQKYQIDELGEEIRPCRLKKRPPVR"
     gene            complement(6688..10122)
                     /gene="dnaE"
                     /locus_tag="WJL14_000701"
     CDS             complement(6688..10122)
                     /gene="dnaE"
                     /locus_tag="WJL14_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ7655802.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKTCRGAGIKPIGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPGQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESIIAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGELVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10580..10894
                     /locus_tag="WJL14_000702"
     CDS             10580..10894
                     /locus_tag="WJL14_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ7655803.1"
                     /translation="MCCRFCGNFGYGGGRCYNNGLFLQGNIMEATVYLEDNEYIALCD
                     LLKLAGLAESGGQAKAFIAEGLVLRNGETETRKTAKIRGGEVIEFDGARLEIADGYDP
                     EV"
     gene            10872..11081
                     /locus_tag="WJL14_000703"
     CDS             10872..11081
                     /locus_tag="WJL14_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655804.1"
                     /translation="MDTTLKYKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(11123..11365)
                     /locus_tag="WJL14_000704"
     CDS             complement(11123..11365)
                     /locus_tag="WJL14_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655805.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            11374..12210
                     /locus_tag="WJL14_000705"
     CDS             11374..12210
                     /locus_tag="WJL14_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ7655806.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(12262..13554)
                     /locus_tag="WJL14_000706"
     CDS             complement(12262..13554)
                     /locus_tag="WJL14_000706"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ7655807.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFKLSDGTSAEKAILLPLAPEYIGYADVHIEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13612..15522)
                     /gene="pglD"
                     /locus_tag="WJL14_000707"
     CDS             complement(13612..15522)
                     /gene="pglD"
                     /locus_tag="WJL14_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ7655808.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMTPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVTPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPDITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(15570..16745)
                     /locus_tag="WJL14_000708"
     CDS             complement(15570..16745)
                     /locus_tag="WJL14_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ7655809.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGSECREFEKEF
                     AAFAGTRYAVALANGTLALDVSLKAIGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAIIVVHLAGMPAEMDSIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKDLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRMPEWTARRQAHAAKLAESLG
                     KFKSIRLIEVADYIEHAQYKFYAFVKPEHLKDGWTRDRIVNELNTRKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTGLMFLVHPTLTGGEIAFCKEHIEAVLAEATR"
     gene            complement(16875..18116)
                     /locus_tag="WJL14_000709"
     CDS             complement(16875..18116)
                     /locus_tag="WJL14_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HEZ7655810.1"
                     /translation="MSKAVKRLFDIIASASGLIVLSPVFLILAYLIRKNLGSPVFFIQ
                     ERPGKGGKPFKMIKFRSMRDAVDKYGNPLPDSERLTDFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMRPGITGWAQVNGRNALSWDEKFSCDVWYI
                     DHYSFWLDMKILFLTVKKVFIKEGISAEGEATMPPFTGNRKLAVIGAGGHGKVVAELA
                     AALGTYGEIVFLDDRTQGSVNGFPVNGTTLLLENSLSPEQFDIAVAVGNNRIRRQIAE
                     KAAALGFSLPVLIHPDAYVSPSATVGQGSVVMAKAAVQAGSVLKDGVIVNTAATVDHD
                     CLLDAFVHISPGAHLSGNTHIGEESWIGTGACSRQQIRIGSRATIGAGAVIVCDIPDG
                     MTVAGNPAKPLAGKNPETGTA"
     gene            complement(18109..>18447)
                     /locus_tag="WJL14_000710"
     CDS             complement(18109..>18447)
                     /locus_tag="WJL14_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655811.1"
                     /translation="YSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQ
                     TNAEVLSLLNLLPHENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKI
                     VNLIERLANE"
BASE COUNT         3815 a         4572 c         5136 g         4924 t
ORIGIN      
        1 cattttgcaa gattttagtt taatcatcaa acagggaaca gcaaaaatcc ccgctgcaac
       61 ggggattttt gttcggctct cgcctgtgtg attaacttca ctaggagaag ctaaaatgct
      121 taaattcctt atgcgtggga aaaagttgct gctgtccgtt gaagtgaacc acaagttcat
      181 tttagcggtt gctttgctgt taagtcaatg cagtagctaa cccgaccaca atcaaaaaaa
      241 cgcaagtttt ccgcctactc tgtgagtggg cggttttttc ttatttcaaa tctgtgacac
      301 aatatctgtc gcctttcttg ccataattgc actgtgtctc aatgacttta acgcacgttc
      361 gcttatcgca acggctggtt gtagcgccca ttctcatttc gcagtgctac aattttttgt
      421 atccattccc agccggaaat taagttgtag ctccctttct catttcgcag tgctacaatc
      481 ttctgccttt ttacaagctc gctttctttg ttgtagctcc ctttctcatt tcgcagtgct
      541 acaattttgg taaaggtttc tgttgcgacc cgaatgttgt agctcccttt ctcatttcgc
      601 agtgctacaa tctttaactt tgaccgtgtg caatccagtt agttgtagct ccctttctca
      661 tttcgcagtg ctacaataac ggcaacccac taaattttgc aaatgcggtt gtagctccct
      721 ttctcatttc gcagtgctac aatagtattc gtcgatgatg gaaactcgag catgttgtag
      781 ctccctttct catttcgcag tgctacaata tgatgcaggc tgctccgtca tcgtctgcag
      841 ttgtagctcc ctttctcatt tcgcagtgct acaatccaaa tagaaataca tacgccgagt
      901 aattagttgt agctcccttt ctcatttcgc agtgctacaa ttttcttttt gtaattgttc
      961 tgcctttttt agttgtagct ccctttctca tttcgcagtg ctacaattac ccacggcgga
     1021 aaccattgcc acaaaacgtt gtagctccct ttctcatttc gcagtgctac aatttaaaat
     1081 aataacccaa tacacgatgt taagttgtag ctccctttct catttcgcag tgctacaatt
     1141 cctgccaatt atcatacata agattttgcg ttgtagctcc ctttctcatt tcgcagtgct
     1201 acaatttgcg tctatggtgt cctttgagat gtccagttgt agctcccttt ctcatttcgc
     1261 agtgctacaa taaaaccaac cgggtattca tacacagcca agttgtagct ccctttctca
     1321 tttcgcagtg ctacaatccg ttgatgacgg atgtgtcggg ttgcggagtt gtagctccct
     1381 ttctcatttc gcagtgctac aattcggccg ctttatttct gccatatcga cggttgtagc
     1441 tccctttctc atttcgcagt gctacaatcc tatgcctgtt attgttttca gccttttggt
     1501 tgtagctccc tttctcattt cgcagtgcta caattttaaa accgcaaacc agtttgtatc
     1561 ctatgttgta gctccctttc tcatttcgca gtgctacaat aataacaacc attttgttat
     1621 ttcgattttt gttgtagctc cctttctcat ttcgcagtgc tacaattccc gacattgacg
     1681 gaaaaacccg aaagcagttg tagctccctt tctcatttcg cagtgctaca atttatgccg
     1741 ttttcatccg gcttgaacat ttgttgtagc tccctttctc atttcgcagt gctacaatac
     1801 ttcgacggga aatccttatt tcataagggt ttcccgtttt atttgttatg aaaaaatggg
     1861 cttaaaataa caataattgg tctgaattga cctttttttc ttgggttttc agctcgccca
     1921 acagcagttt catggcggca tattgctttt ctgttatctc caaacagcgg attgagcctt
     1981 cttgcggaag gtttgcacac agtctgttgt ggtgtttttg caacgaatcg cggcctttga
     2041 cgatacggct gtatacggaa agttgcagca tttggtatcc gtcttttaat aaaaactggc
     2101 ggaattgatt ggcggctttg cgctttgccg ccgtgataac cggtaggtcg aagaagacga
     2161 taatcctcat aaatttggcc tcactcatat tggtattctt tcaatggtag gatttcaggc
     2221 agtttcagtt gtttggcgtt tttatcggtt acgccggctt gaaatgacga aatcattttg
     2281 tcgatggcag ccaaggtgct gaaatgttgc cgttcgatgc tggtttgata gtagaggatt
     2341 ttgatgaggt gttgtttgat gccgagcgtc agttcagttt tcagacggcc ttgttcgtac
     2401 aaatgtatca cggtcaggtc ggccaatggg cgtaagggtt cgataaagtc atcggcgaga
     2461 ttaaatggat tgagttcact tctatgaaac aagcctaatg caggtaacca accgtataag
     2521 gtcagggcgc gtgcaactgc ggcacggagt acggcgtagg tgtagttcaa tgcggcattg
     2581 acggcgttgt tgtcattacg ggtaaatttt tcgccaaaga gggcttgaaa gtagagggcg
     2641 gcagcttggg cttcacggtt tcctgtatcg cctgaacgga cttcagatgc caaagtgcgc
     2701 agccgtttgg ctgcaagatc attgcctgtt tcgtctgcga caaatgcttg gttcaggatt
     2761 ttttggcgga cgatatgttg ccagagttgc ttttttaagg gttcgcttat gttcagttgc
     2821 agtttgagga ttttaagttg gcggtgatat tgggcataag gcagccattg tccgcagggc
     2881 aggaactgct cgtcgcaggt cagcagggtt gcaccatgct cggctaaggc tgataggagc
     2941 ggggcggtaa tcagggtttc gcgattttcg atgatgataa cggcgatgtc ctctaatggt
     3001 acggtgtggg actcgccgtt ttgttgaatc agcagttgcc ggcgttgcaa ggatagtttg
     3061 ccgccgtttt gaataagcag gctgcgccaa gtcatgtttt tccccttatt tttatataaa
     3121 aaatccctgc ttcaggccgc ctgaaacagg gatagattaa tttaacggac aggcgggcgt
     3181 tttttcagac ggcatggtct gatttcttcg cccagttcgt caatttggta tttttggaat
     3241 gaaagggcgg ttttgacgcc gataccttcc agtattccat ttttgccaat tttatgatca
     3301 agatcatgaa tgcgtatatt gatattaccg gtgcctcgat ggcagctggc aaagtaacca
     3361 aacattctag ctttttttgt tataacctcg actaaatcat tagggtgtaa tgagaattta
     3421 aagttgaaac tatcatcaat aagttgccaa tcttcttcat cttttccttg tacaacagcc
     3481 ctatccggca aaatcccttt cgctacctgc caactgtaaa tcggtaccag ataatacttg
     3541 tcgcctttct caaacacatc tacgcgcacc atggttgcgt tgtcggcaat accgttatgg
     3601 ttgcgcaccc atacgccggt tttctgtact tgctctacgc gtacggcttt tacctgttgg
     3661 gtgcggttgc ctgctttatc gtatttgtaa aacggctcgg caaaggcttt ggcaggatcg
     3721 tctttatgtg cttccagccg tgctttcagt gcttcgtata gcttaggttc gcgctcccga
     3781 ttgaccattt tttccaagtc tttcagtttt aactgtgtta acggtacgcg caacacgctg
     3841 acgccttcgt ccagtcgttt ggcggatttg acggtctcca tatgcccttg cccgctcatc
     3901 ttccgattgg gcgcgcgtga aacaaacagt ggcgtaacgt attcgtgtac ggcttcgggg
     3961 cgagatgata atttttcggc aagcaacgtg cgcagttttt ctggggtatc ggcttcttcg
     4021 aattcgggtt tgccgtccgg tttgccgaag acgcgaatca tgacttcttg tgcgaaaaat
     4081 tcccaaggtt gtgggaagtg tgttttttga tgcagcactt ctcctgtttc tttgtctatg
     4141 gttttaccgt caaacgcgtt catctcttta tagcgtacaa aacgggtaat tttctgctgc
     4201 atggcaacgg tcgagcaggc aacgacgacg gcgtccaagg cgtgatggcg gtcgttttcc
     4261 gcacgcactt tgcgcaatcc ccaaaagccg cgcaacagat tggtaatttg tccgttggat
     4321 gcaaagacac gtttcttgcc tttacctgtc agccgcatac ggtcggcaac aaattgacac
     4381 aggaaacggt tgacgtagcg cgtgtcgttc agattgcgtt ctttaaagcc gtcttcatcg
     4441 aatttttgca gcagaatccg ttgtttttta ctgcgcggga aacggctggt ttcgacacgc
     4501 gctttaaatt cctgccattc gcggctgttg tctttgccgt tgaagtattc gtaaggggtt
     4561 tgattgcctt tgttttggtt ttcgctgccc aataccagta ctttattgtt gaaactgtcg
     4621 tcccatgtgc gcgagaacgg cagggcatgg tcgatttcga catagccttt ttcgttcaga
     4681 cggcctaagt tgatttcttt gcccgaatac aggcatttgc cgtgttgttg ctcgtacagg
     4741 cgcagtttca gaatatcttt ggatttgggt tctccgacaa aattggggaa atactctcgg
     4801 aatttggcgg cggctttttc cctgtctttg cggttttctt cttggcgttt ctcaatttct
     4861 ttgcggtctt taaacgattt acctacttcc cttgcagttt caatatggat acgagctggg
     4921 gagccgtaac ggcgtaccac gccgttaatg accttacgtg cttgagataa ggcgcgcaag
     4981 acgacggggt tgcggatttc gtcggcggga atcggcggca gataaatctt ttcttccgta
     5041 ttcttcttgc cgtaatggtc tccgtagatt tcggcgcagg cttcatcgta acgtttgcct
     5101 tgttccatta gaggcacaat tcggcgcaat gctttcaagg aaatttggac gaacttatcg
     5161 aagctgatgt gtttcaacag cgcttctaag atttcgggct gtatacggtc tttcagacgg
     5221 cctgtaatgt cttcatcggt tttgaacagg gagaatgccg tgccgatttc gtcttgtaat
     5281 tcgggagaaa ggtttaatgg ggattttttg tctttcaatc cttctttttc cagtgcacgg
     5341 ctgatggcat ggtaggcctt catttccatc aatgttgagg cttcggcatt gtctttacca
     5401 tagcgcaagc ctttgaaaaa ggcggtatct tctaaaccca gcagcttacg ggcttgtgcg
     5461 taagtcagtt tggattttct gtatggctcg tccataagcg tggcgcgttc ggtatcggtc
     5521 aatggccgct cgctgccttg ctctaaaata cgcaggttgt tcagcttggt cagccagatg
     5581 aaacgttcgg ctgtgtaggt gtttttagcg gctttcggct ctgccggttc gaaggtgcaa
     5641 tgccccaaca ttttttgaac ggcatcgccg gacagggcag ggcgttgcgt catcagtagg
     5701 gtttcaatac cttctttaag gccgcctgaa acatgcggat tgccaaattc tttttgtttt
     5761 tcaaacagca aaatcagctc cgcctgtaaa tctttgcggc tgaacgtatg cgaataatcg
     5821 ccgcgctgat tgcggatatg gccgctttct ttctcaaatt tatttaaagc caattcggcc
     5881 ggtgtgcgga aatcgcctgt ctgtaaggca tgggcattgt cggctacgcc tttgagcaaa
     5941 gcgccaagct ctttatcggc agtttcgccc tcgttttttc tttgcgacaa ataaccgcgg
     6001 tgtttgatta aatgcaacaa gactgctgac cactctaaag gcgtcagttt gcggtctaat
     6061 gcggctgcgc gaagttgcca tggtgtattc ggtaaggatt tgatcaagcc gttttcgtca
     6121 aaatcagcgg cttgtaatac gccttcgcgt ttcaataggc ggcgggcccg aagcaggcga
     6181 tgggcgcgac ggcgggtcag gcggcggaca ctgcgcgcca aacgccttgc catggcaagg
     6241 gagtcgcctg ttttcggtac ttcggcacgc tcaaatacgc gcacgcccaa atcaatcagg
     6301 cagattgggt tttcatcttc gtcaatttct accatcgccc agccgacgga tgcgatgccg
     6361 atatcgaggc cgaggatgta gttgattgga ttaggtttga aggcagccat ttattactcc
     6421 gtaaacaacg ttgcaaataa tgataatata tcttattgtc ttatttatat acaaatagat
     6481 tattgactta tcatctcaca acggctagaa tcccaaacat attgtcgcac tgcgaaatga
     6541 gaaccgttgc tacaataagg ccgtctgaaa agatgtgccg caacgctctg ccccttaaag
     6601 cttctgcttt aaggggcatc gtttatttcg gttaaaaaat gccgtctgaa accgattttg
     6661 ggcttcagac ggcatttata ttttgggtta ccagttgacg cgcaccgacc ggctgccgag
     6721 caatgtttcc agttcgccga acaatgcgga gctcggtgta accgtccatt tcggcggcac
     6781 ttgaagcctg cccgacgctt tttcgttggc atacgacagt tgcagcggga tgcgcggcgt
     6841 gtcgggcagt tggtgggcgg caagcagttg taccagttcg ccgatgtcgt gatgcggggc
     6901 gagggcgagg ctgaggctgc gggcgtagcg ttcgcgcgcc gtttgcaggg tcatgacttg
     6961 gtttgccaga atacgcagcc cgtcgccgcc gccgtagtcg tcgcggctga ctttggattc
     7021 gataatcagc acttggtcgg ctttgaggca gtcggcgcag ttttccaacg tctgaccgcc
     7081 gaccatgatt tcaacctgtc cgctcaaatc ttcaaggctg acgaaggcga ttttgccgcg
     7141 cttgcccatc atcgtacgca cggcggtaac gaatccggcg aggcgcacgc tgtcttgcgg
     7201 tttgagtttc gacagtttct gcggggcgat ttggcggact tcttgggcat acgggccgaa
     7261 cgggtggccg gacaggtaaa agccgatgac ggttttttct tcggcgagtt tttccgattc
     7321 gctccacatc ggcgcgtcaa tgagccgcac cggttcgatg gcgtcttcca tcatgtcgaa
     7381 aagcccgccc tgattggcgt tggcggcttt ttggtcggcg ttgttcatgg cgaggtcgat
     7441 gtttgccaag agcatggcgc ggttgggttc gatgctgtcg aacgcgccgc cgcgtatcag
     7501 ggcttcgagg gtgcggcggt tcatatgttc tttgccgacg cgctcgcaga agtccaagag
     7561 gccggtaaac ttgccgccgc tttgccgcgc ggcgatgatg gattcgacgg cggcttcgcc
     7621 cgtgccttta atcgcgccga gtgcgtagcg gattttcatg ttcggatacg gcgtgaagcg
     7681 gtagtcggat tcgttgatgt cgggcggcag gaactcgatg ccgttggcgc ggcagtcgtc
     7741 gtagaaatgc ttgagctggt cggtgttgtc caattcggac gacatcgtcg ccgccataaa
     7801 ttcggcgggg tagtgcgcct tgagccatgc ggtctggtag gaaatcaggg cgtaggcggc
     7861 ggcgtgggat ttgttgaaac cgtagccggc gaatttttcc atgtagttga agatttcgtc
     7921 ggatttttcg cgcgaaatgc cttgtttcgc cgcgccttcg gcgaagattt cgcggtgttt
     7981 caccatttct tcgggctttt tcttacccat cgcgcggcgc agcaagtccg cgccgccgag
     8041 cgagtagccg ccgataatct gcgccgcctg catcacttgt tcctgataca ccataatgcc
     8101 gtaggtcggc gcgaggatgc cttcgagcag cggatggatg tattggaatt cctggccttt
     8161 catgcgcgcg acgaagtcgg ggatgttgtc catcgggccg gggcggtaga gcgatacgaa
     8221 ggcaatcagt tcttcaaact tggtcgtatg cgccgttttc agcatttttt tcatgccggt
     8281 cgactcgaac tggaacacgg cggtggtgtt cgcatctcgg aagatttggt aggcggcttg
     8341 gtcgtcgagc ggaattttgc cgacatcgac gatatcgccg gtggtgtttt tgatgttgtt
     8401 ctgcgccatt tcgataatgg tcaggttacg cagacctaaa aagtcgaatt tcaccaaacc
     8461 cacgtcttcc acgtcgccct tgtcgtacat ggatacggga gaggcggatt catccgcctg
     8521 atacacgggg ctgtaatcgg aaatcttgcc cggcgcaatc aacacgccgc ccgcgtgcat
     8581 acccagaccg cgcgtcaaat cttccagctt tttcgccagc gtaatgagtt cgtccgcttc
     8641 ttccgcttcg attaattcct gaatctgcgg ctccgccttc atcgcgtcgt ccaaacccag
     8701 aggtttgttg gcttccaacg gaatcagctt ggacagtttg tcgcacagca taaacggcag
     8761 ctctaacacg cgccccacgt cgcggatgac cgctttagac gacatcgtgc cgaaggtaac
     8821 aatctggctg accgcctccg cgccgtattt ttcgcgcaca tactcaatca cgcggccgcg
     8881 gtttgcctga cagaagtcaa cgtcgaagtc gggcatggag acgcgttcgg ggtttaagaa
     8941 acgttcaaac agcagcgcgt atttgagcgg atcaaggtcg gtaatcttca atgaatacgc
     9001 caccagcgag cctgcgcccg aaccgcgccc cggccccacc ggacagccgt gcgtttttgc
     9061 ccagttgata aagtcttgta cgataaggaa atagccggga aatttcatct ggatgatgat
     9121 gttcagctca aaatccaaac gttcctgata ttccggcatt ttttctgcgc gttccgcttc
     9181 gtcgggataa agctgaacca tgcgttcctg caaaccctcg ttggagagtt tgatgagata
     9241 gtcatcgagt gataaaccgt cgggcgtggg gaaaaggggc aggaagtttt tgcccaatgt
     9301 gatgtgcagg ttgcagcgtt tggcaatttc taccgtgttt tccaaggctt caggcaaatc
     9361 ggcgaaacgt tcggccatgg tttccggcgg aatgaaaaac tggcccggcg tgaaatcgcg
     9421 cggacgtttt ttgtccgtca atacccagcc gcctgcgata cacacccgcg cctcgtgcgc
     9481 gttgaaatcg tcgcggctca taaactgcgt cgggtgggtc gccaccaccg gcaaacccag
     9541 ttcctccgcc agcttcacgc tgccggaaac gcaagcctcc cattcggggc gttcgggcag
     9601 gcgttgcagc tctaaataaa acgcatcggg aaaccatgcc gcatatttca acgccgccgc
     9661 acgcgccgcg tcttcattgc cgttcaagag attcacgccc acttcgccgt aatgcgcgcc
     9721 gcttaaacaa atcaagccgc tgttgtcgcc gttttccagc cattcgggat tgagttccgc
     9781 atgatggata ttgcggtctt tgccgacata agccgccgtc agaagctcgc tcaagcgcag
     9841 atagcccgca tcgttgcgga taatcagcat ggcgcggaac ggcttgtcgg gcgcattcgg
     9901 attgcctatc cacacgtccg ccgcgccgat gggtttaatc cctgcgccgc ggcaggtttt
     9961 atagaatttc accaaaccga attcgttcat caaatcgctg atgcccaaag caggcaaacc
    10021 gtattcctgc gctttggcaa tcagtttttt aatccgcacc ataccgtcgg taatcgagaa
    10081 ttcggtatgc aggcgcaggg ggatgtaggt cggctcggtc atggcaaaat cggcgtgaac
    10141 aataaaaggc gtattgtagc agggttgtct ttagatggcg gtgtaggtaa tgctgtttcg
    10201 gattcagacg gcatgacctg caaatgtttt tgagctttta cgacggcaaa aaaatgcctc
    10261 ctaccgtgtg gtggaggctt cccaaggagt attgatagat ataaaggact atcaaactag
    10321 ttataaagaa ctatatacat tattcggacg gatggcaagc agttaaatcg attttacgtt
    10381 caaacaggtt tttgatttcg ttttgatgcc gattgccggt gtatcgggca gttcgcgttt
    10441 gaggatgtgc atcagcgtca atgcggattc gtcggggaac aggatttgca gcttgccgtt
    10501 ggacaggatt tctttgatcg gatggcggca tatggggttt cagacggcat ttttactttt
    10561 acggaacagc tggtgtggga tgtgctgccg gttttgcggt aattttgggt acggcggcgg
    10621 caggtgctac aataacggcc tctttctgca agggaacatt atggaagcca ccgtctatct
    10681 cgaagacaac gaatacatcg ccctgtgcga cctcttgaaa ttggccggac ttgccgaaag
    10741 cggcggacag gcaaaagcat ttatcgccga agggctggtg ttgcgcaacg gcgaaaccga
    10801 aacccgtaaa accgccaaaa tacgcggcgg cgaagtcatc gagtttgacg gcgcgcgctt
    10861 ggaaatcgcc gatggatacg accctgaagt ataaagccga agcccttttg ggcgagccgc
    10921 ttttagaaga acccgtccgc cctgagttgt gggaatgctg tggcagcgac tgcggcgagg
    10981 cgtgcattca gacgatttac tgggcggata aggcacgcta cgacgcgcaa cggcggaagt
    11041 tgaaagaagc gggttgggcg gaagacgaaa atgccgtctg aaaccagttc ggcttgataa
    11101 aaccacgttt tcagacagtc ttttatagta gattaaaatt gaaaacggtc atatcgccat
    11161 tccctcgaaa gcaggaatcc ggaagtttga aatagcggtt aaccttaaac atttccaata
    11221 aatcaaagtc tggattcccg cctgcgcggg aatgacggca tagcggcttt tcctttgcat
    11281 ttgccataca cttgtttttt gaaatcaccg ccgccacacc gcccgacaat attgaaacac
    11341 cctgcccacc atttaccacg cacataacgc cttatgatag acctgcactg ccattccacc
    11401 gtttccgacg gtatgctctc ccccgccgaa gttgtgcgcc tcgcgcatca aaacggctgc
    11461 acgctgctgg cgttgaccga ccacgaccac accggcggca tcgccgaagc gcgtgccgaa
    11521 gccgacaggc tcggtttgcg cctgattaac ggcgtggaaa tctccgtaac gtggcgcggg
    11581 cgtaccatac acgttgtcgg cttagacttt gacgaacaag acgaaaacct gcaaaacctg
    11641 ttggcgcaag tgcggcaagg ccgtctgaaa cgcctcgaag ccatcgccgc caagctggaa
    11701 aaacgcggca tttcgggcgc atacgacggc gcgctcgcgc tggcggcaaa caaagaaatg
    11761 gtcagccgca cacacgtcgc cgagttcctc attcgggtgg gacacgtcaa aaacaagcag
    11821 caggcgttca ccaaatactt gggcgacggc aaatcctgcg ccgtccgcca cgaatgggcg
    11881 acgctggaag actgcgtctc cgccgtcaac ggcgcgggcg ggttggcggt catcgcccat
    11941 ccgatgcgtt atgatttgtc cgccaccgcc aagcgcaatc tgtttgaaga atttaaaaac
    12001 ctcggcggcg caggcatcga agtccacagc ggcaactgct gcaaaaacga ccgcctcaac
    12061 tacgcgcttt tggcagaacg cttcggtatg ctcgccagcg cgggcagcga cttccaccgt
    12121 ttaaacgact ttagcggcgg cattctcggc gcgtgtcccg aactgccgga aaactgcagg
    12181 ccggtgtggg cgcatttttc ccgatattga atgcagataa aaatgccgtc tgaaaacctt
    12241 tcagacggca ttttttgcgg tttaaacgtt gtcgtacagt tttcggacgg ttttgccgat
    12301 ggcggcgatg cctttttcca gcgtttgagc gtcctgcgcg atgctcatgc ggatgcactc
    12361 gcccgcgtgc gggtaatccc gcgtgtcgat gccgacgaag aaatgctcgc ccggaataat
    12421 cagcgtacct tcggctttga gcatttcgta cagggtttgc gaagaaacgg gcaggttttc
    12481 aaaccagagc cacaggaaaa tcgcgccttc gggtttgtgg attttcatcg ggtacgcgcc
    12541 cagctcgcgc ttgagcagcg agacggcggt ttgcgcctga ttgcggtaaa acggccggat
    12601 gacttggtcg gcaagccgtt tcatctcgcc gctttccagc agcggggcgg cgatggccgc
    12661 gccgaagcgc gtgggggcaa ggttcacaat cgcgttcagg ctgctgacgg ctttgacgac
    12721 ttcgggcgcg gcgacgatga tgccggtgcg aacgcccggc aggccgactt tggaaaggct
    12781 gaagcagagg atgatgtttt cgtgccaatt cagcgttacg tcgctgtaaa tgatgttggg
    12841 gaacggcatt ccgtaggcgt tgtcgataat cagcggaatc ccgtgttcac gcgccaaagc
    12901 gtccaaacgt gccatttcgc cgtcggtcaa cacattgccg gtcgggttgg tcgggcgcga
    12961 acagcaaatc gcgccgattt tgcccgcttt gagttcgggc aggttttcca gtgcgtcaaa
    13021 gtccacgcgg tatttgaaga agcctgcttc gccttcgtgt tcgacgtttt cgattttggg
    13081 cttgacagaa acgaagtgct gcccttcgat atgtacgtcg gcatagccga tgtattcggg
    13141 cgcgagcggc aacaaaatgg ctttttctgc ggatgtgccg tctgaaagct tgaatttgcc
    13201 gccgaagagg ttgaataaat agaaaaacgc gttttgcgaa ccgttggtca gcgcgatatt
    13261 gtcggcggtc aggttccagc cgtattcgcg gttgaggaag gcggtcaacg cgtcaatcag
    13321 cgcggcatcg ccttgggggt tggagtagtt gccgatgttt tcgacggcgt gttctgccgc
    13381 cagtttggag aatatgtcgg cgaacgcccg atcgatttcc ggaatgcgcg ccgggttgcc
    13441 gccgccgagc atgttgacgg gcttgtcgct tttgagcgcg tcgccgaggt cgtccatcag
    13501 ttggaggatg ccgctgtgtt gcgtgaattt ttcgccgaat gccgaaaact gcatgtcaaa
    13561 ctccgtgtgt gtgtaaggta ggttggtttg tacgttatgc cgtctgaagg ttcagacggc
    13621 attttttctg tgtgtttctc gtacccaaag caggtcgcag ataccgtcgc ttggggtaaa
    13681 gccggtcggg gcgttgatga gcagggtgcg gattgcctgc tggtcgtaac ggtcgcaggc
    13741 cgcacggatg cggtcgagca ggacagagag ctcgtgccac ggcagcatcg cctcgtcagc
    13801 cgtcataatg cgcggatggc cggttttgcg gacgttgtcg ccgatgagca gctcttcgta
    13861 gagtttttcg ccgggacgca gtccggtaat gaggatttcg atgtcgccgt cgggttgttc
    13921 gggtgttttg ggtttgaggc cgcttaaggt aatcatttgg cgggcaaggt cgatgatttt
    13981 gacggattca cccatgtcga ggacgaatac gtcgccgccc gtacccatcg cgcctgcctg
    14041 tatgacgagt tgggcggctt cgggtatggt catgaaataa cgtgtgatgt cggggtgggt
    14101 cagggtaagc gggccgcctt ctgcaatctg tttttcaaac agcgggacaa cggagccgga
    14161 cgaacctaaa acattgccga aacgtaccat gctgaagcgg gttttctgtc cgggttcggc
    14221 ggcgagtgcc tgaaggcaga gttccgccat gcgtttgctg gcacccatgg tgttggtggg
    14281 gcggacggct ttgtcggtgg agatgaggac gaaagttctt acgcccgatg tcgtggcggc
    14341 aagcgcgcac tcgagtgtgc cgaagatgtt gttgcgtatg ccttcgacgg tgttgaattc
    14401 gaccatgggg acgtgtttgt aggcggcggc gtgatagacg gtcgcaacgg aaaaggcggt
    14461 catgacgtgt tcgagcagcg tgcggttttg caccgaaccg agaaagggca ggatttcggt
    14521 gtcgaggcgt ttttggatgc aggtttcgcg caattctttt tcgatggcgt acagggcgaa
    14581 ttcggataac tcgaacagca gcagcttttc ggggcggcgg cggataatct ggcggcagag
    14641 ttccgaaccg atggagccgc ccgcgccggt taccatgacg gttttgcctt cgatgtcggc
    14701 actcatcagg cggtcgtcgg gtgtgacgga atcacgcccg agcaggtcgg acacggagat
    14761 ttttttgagc gtgccgatgc tgattttccc gtccatcagg tctttcattc cgggaatggt
    14821 caacacttcg cagggatagg cttccagttt gttgatgatt cggcggcgtt gttcctgggt
    14881 cgcgccgggg atggcgagca ggattttttc cacgccgtag cgttcgatga ggaaggcgat
    14941 ggcatcgggc tggtaaacgg caaggtcgta gatgacggtg tgccacagtt tggggtcgtc
    15001 gtccacaaag gcggcggcgg aatattcgcg catttgtttg acggcctcga gaagctgtct
    15061 gcccgcctgc cccgcgccgt aaataatgac gggggtcatc tgttttttgg ggtgttcgga
    15121 caacagcccg cgcaaaacca tacgcgagcc ggtcacggaa acaaacagca gtaagaaata
    15181 gacaatcggc agggcgaggc gcagcctttc ttcaaaaatc aatgtgttga ggaagaacaa
    15241 tacggcggag gcgaggctgc ctgccagcgc ggtggagagg atgcggaagc tgatgaagcg
    15301 tgtaacggca cggtagagcc ccattctgac gaacagaccg atggtcaaca gcgcggtcaa
    15361 taggaaagac tgccagttgg cagggtcgaa ccattcgttc gagtagtcgg cctttaggct
    15421 ttgggtgaac caaaaggcaa tgaaaatcat cagaaaatcg tgtatgagga aacagatttt
    15481 cttgatgttg cgcggcaggg cgatcagggt ttccagagtc atatcgtggg gcggtatgtg
    15541 ctttcaggcg gcatatgccg tctgaagggt tatcgtgtgg cttctgccaa tacggcttcg
    15601 atgtgttctt tgcagaacgc gatttcgccg ccggtcagcg tcggatgcac caaaaacatc
    15661 aggccggtgt cgcccaactc gacagcgttt ttcagccgct ctttgggtcg ccacggcgtg
    15721 ttgtcgaagg ctttttccaa atagacttcg gagcagctgc cttgatagca ggggactttg
    15781 cgcgtgttca gttcgttgac gatgcggtcg cgcgtccagc cgtctttgag gtgttcgggt
    15841 ttgacgaagg cgtagaactt atattgcgcg tgttcgatgt agtcggcgac ttcaatcagg
    15901 cggatgcttt tgaatttgcc caaactttct gccagcttgg cggcgtgtgc ttggcggcga
    15961 gccgtccatt cgggcatacg tttgagctgg atgcgcccga ttgccgcctg catttccatc
    16021 atacgccagt ttgtgccgaa actttcgtgc agccagcgga agcccggtgc gtgttcgcgg
    16081 tggtacacgg catcgtagct tttgccgtgg tctttgtacg accacatttt ttcccacaaa
    16141 tccttgtcgt tggtggttac cataccgcct tcgccgccgg tggtcatgat tttgtcttgg
    16201 cagaacgacc acgcgccgac gtgtccgata gagcctacgg atttgccttt gtatttcgcg
    16261 ccgtgcgctt gggcgcaatc ttcgatcacc cacaaatcat gttcttttgc caaagccata
    16321 atgctgtcca tttcggcggg cataccggcg aggtggacga cgataatggc tttggtattc
    16381 ggcgtcagcg cggctttgac ggtttccgcg ctgatatttt ggctgttcaa atccacatcg
    16441 gcaaacaccg gatttgcgcc cgcgttcaca atgcaggatg cggaagccag gaaagtgcgc
    16501 gaagtaacaa tcacatcgtc gcccgcgcct atgccgattg ctttgagcga gacatcgagt
    16561 gccagcgtgc cgttggcaag ggcgacggcg tatcgcgtgc cggcaaaggc ggcaaattct
    16621 ttttcaaatt cgcggcattc gctgcctgtc cagtaattga ctttgttgga cagcaggact
    16681 ttggaaacgg catcggcttc ttcttgggtg aagcagggcc acggggagag ggaggtgttc
    16741 agcatagtgt tccatccgtc gggtttggac ggcatttccg accctatgcc gtctgaaggg
    16801 gatgcgcccc gaaaaatcgg gcgtgcgccg caggtgttgt caaaaccgct cggtgcggtg
    16861 tgtattttaa tcgcttatgc cgtcccggtc tcggggtttt tgcccgcaag gggttttgcc
    16921 gggttgcccg cgacggtcat gccgtccggg atgtcgcaca cgataaccgc ccccgctcca
    16981 atggttgcgc ggctgccgat acggatttgc tggcggctgc atgcgcccgt gcctatccag
    17041 ctttcttcgc cgatatgcgt gttgcccgac aggtgcgcgc ccgggctgat gtggacgaaa
    17101 gcgtcaagca ggcagtcgtg atcgacggtg gcggcggtgt tcacaatcac gccgtctttc
    17161 aatacgctgc ctgcctgtac ggcggctttc gccatcacga cgctgccctg accgacggtt
    17221 gccgacgggg aaacatatgc gtccggatga atcaggactg gcagggagaa accgagtgcg
    17281 gcggcttttt cggcgatttg gcggcgtatg cggttgttgc cgacggcgac ggcgatatcg
    17341 aattgttcgg gcgataaact gttttcaagc agcagcgttg tgccgttgac gggaaagccg
    17401 ttgacgctgc cttgggtgcg gtcgtccaga aaaacgattt cgccgtatgt gccgagtgcg
    17461 gcggcaagct cggcaacgac tttgccgtgt ccgcccgcgc cgataacggc aagtttgcga
    17521 ttccccgtga aagggggcat agtggcttcc ccttccgccg aaatgccttc tttaataaag
    17581 acttttttga ctgtcagaaa caggattttc atatccagcc aaaaactgta atggtcgatg
    17641 taccaaacat cgcaggagaa tttttcatcc cacgaaagcg cgttgcgccc gttgacctgc
    17701 gcccagccgg taatgcccgg tctcatttcg tggcggcggt tttggaagtt gtcgtacagc
    17761 ggcagatatt gcatcagcag cgggcgcggg ccgactaggc tcatgtcgcc tttgaggacg
    17821 ttccacagtt cgggcagttc gtccaggctg gcggcacgca gttttttgcc gaaatcggtc
    17881 aggcgttcgc tgtccggcag cgggttgccg tatttatcga ccgcatcgcg catagagcgg
    17941 aatttaatca ttttaaacgg tttgccgcct ttgcccggcc gctcttggat gaagaacacg
    18001 ggcgagccta gatttttgcg gatgaggtac gccaaaatca ggaaaacggg tgagaggaca
    18061 atcagccccg atgcggatgc gatgatgtcg aacaggcgtt tgacggcttt actcatttgc
    18121 caatctttca atcaggttga cgattttccg ataggaaatg tcgcgcctga agcggcggac
    18181 gatttcatcg gactgaacgg ggtcgttttt gcgtttcaaa atatctttgg cggcttgaac
    18241 gaagctgtcc acgtcgccgg aacggtagtt ttcgtgcggc agcaggttca agaggctaag
    18301 gacttcggca ttggtctggc tgttcagaat cggtttttgc aaagccatat agtcggagag
    18361 tttgttggta accgactgca tggcgtggct atggatggcg ttgacggcaa tgtcgcagcc
    18421 tttggcaatc gacatcattt cagaata
//
LOCUS       DAWKQB010000028        17785 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.028, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000028 DAWKQB010000000
VERSION     DAWKQB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17785
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.028"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(15..1124)
                     /gene="mltB"
                     /locus_tag="WJL14_000711"
     CDS             complement(15..1124)
                     /gene="mltB"
                     /locus_tag="WJL14_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ7655812.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPAFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNVGDVAASIANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1033..1284)
                     /locus_tag="WJL14_000712"
     CDS             complement(1033..1284)
                     /locus_tag="WJL14_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655813.1"
                     /translation="MPSDGIFGGGIRISGGARGNVRHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1326..2753)
                     /locus_tag="WJL14_000713"
     CDS             complement(1326..2753)
                     /locus_tag="WJL14_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7655814.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFADGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AKHEDAAACLLNDIRKDILDCRYCG"
     gene            2938..7050
                     /gene="mfd"
                     /locus_tag="WJL14_000714"
     CDS             2938..7050
                     /gene="mfd"
                     /locus_tag="WJL14_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ7655815.1"
                     /translation="MTYPIPKPREKSRWLNLSQGSLPLALARYLPHKQLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKSDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHTEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHTLPDLAVNRQSDEPLQALKDFQTAFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSAAATNPLPSPLPQEREQSAAVVSDSLKAAAISTESSLPLGTSNLHGQIRQQPAPSP
                     ERESRGESKAVSAQTEFSAAAISPLPSPLPQEREQSAAAVSDGLKTESSLYSIANNLH
                     GQIRQQPTPSPVGEDWGESKTVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTK
                     SSLYPIENSLHGQIRQQPAPSPVGEGWGEGKAVADQSAIAVITESDLYQYVARSRVHN
                     RRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLVTIDLGGETNEMMLLEYAGE
                     AQLYVPVSQLHLISRYSGQAHENIALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAR
                     RAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGF
                     GKTEVALRSAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSK
                     ATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLREL
                     KRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITK
                     DAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQA
                     VRDLKKGHQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQIN
                     AIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGINAIDAAGEAVTVTFGKNNNIDPT
                     EIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7199..7582
                     /locus_tag="WJL14_000715"
     CDS             7199..7582
                     /locus_tag="WJL14_000715"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ7655816.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7604..8446
                     /gene="kdsA"
                     /locus_tag="WJL14_000716"
     CDS             7604..8446
                     /gene="kdsA"
                     /locus_tag="WJL14_000716"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ7655817.1"
                     /translation="MDIKINDITLGNNLPFVLFGGINVLESLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8461..8901
                     /locus_tag="WJL14_000717"
     CDS             8461..8901
                     /locus_tag="WJL14_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ7655818.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMAAALLSIYLSWRLSGKDAV"
     gene            8949..10235
                     /gene="eno"
                     /locus_tag="WJL14_000718"
     CDS             8949..10235
                     /gene="eno"
                     /locus_tag="WJL14_000718"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ7655819.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGK"
     gene            10249..10527
                     /gene="ftsB"
                     /locus_tag="WJL14_000719"
     CDS             10249..10527
                     /gene="ftsB"
                     /locus_tag="WJL14_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ7655820.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10568..10858)
                     /locus_tag="WJL14_000720"
     CDS             complement(10568..10858)
                     /locus_tag="WJL14_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ7655821.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(11056..12189)
                     /gene="nrdB"
                     /locus_tag="WJL14_000721"
     CDS             complement(11056..12189)
                     /gene="nrdB"
                     /locus_tag="WJL14_000721"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ7655822.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12250..13437)
                     /locus_tag="WJL14_000722"
     CDS             complement(12250..13437)
                     /locus_tag="WJL14_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7655823.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGFLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(13430..14443)
                     /gene="dcm"
                     /locus_tag="WJL14_000723"
     CDS             complement(13430..14443)
                     /gene="dcm"
                     /locus_tag="WJL14_000723"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ7655824.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(14501..16780)
                     /gene="nrdA"
                     /locus_tag="WJL14_000724"
     CDS             complement(14501..16780)
                     /gene="nrdA"
                     /locus_tag="WJL14_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ7655825.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCNEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQAISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17309..17620
                     /locus_tag="WJL14_000725"
     CDS             17309..17620
                     /locus_tag="WJL14_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ7655826.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFR
                     R"
BASE COUNT         4481 a         4737 c         4221 g         4346 t
ORIGIN      
        1 ccttttaaaa aacattacaa tcccgggccg ccaagcgaat tggcaatgtc cctgaccgcc
       61 gttacataca tccgactgtg attgtactgc cataccgtat aaaaattgtt caagcccaaa
      121 taatattcaa acacgccggg tgcggtttcc agtttgaaca aaaccgcctt ttcatcatcg
      181 gcaagctctt cgccggggat gatgccgtac gccttcaaat ccgccaccgt ccgcgtcagg
      241 gcggtttttt cgccaatgat tgcctgaaca tccgcacccg gcgccaatgt tgcagacacc
      301 agtattttcc cgcccgtgcg ccaaccgtgc tgcttcatat aattggcaat cgatgccgcg
      361 acatcgccaa cattgcccca tatgtcccga tgtccgtccc cgtcataatc caccgcccat
      421 ttccggtagc tcgaaggcat aaattgcggc atccccattg cgcccgcata gctgccttta
      481 aaggcgaaaa catcgccgcc ctcttctttt gccagcttta aaagctcgac caattctttt
      541 tggaaaaacc cggcgcggcg ggggtaatca aagcctaagg tcgccaatgc gtccgccaca
      601 cggaaactgc ccgtattttt gccgtaattc gtttcaatcc cgataaccgc cacgataagt
      661 tcggcaggca cgccgtattt ttgcgccaca tcatcgataa gcgcgcggtt ttccgcataa
      721 aaccggcgcg cgccgcgaaa tttcgccttg cccgaatttc ccgtgcggaa cacataccac
      781 ggacgcgatg tggaggggcg gtgcataatc ttgacgatgt ccgccttgta agccgctttg
      841 tcaaaaaaat cctgccattc cgcccgggaa aaatcccctt tcccgacttc atcgtccaca
      901 aaacggcgga catttgcatt ggcggcaaac ccgctgtcgg ataccggtac ggctgccgcg
      961 tcaaatacgg ctgccgcgtc aaacgcgggg cggctttctt ttttcatttc atccgcgcgg
     1021 ggggcttggg cttcatttgc ccggggcggg cgtgcctcca tcgccgtaca ggcagacaaa
     1081 gccgccaaac aaattgccag cggcagtatt tttctctttt tcataaatat gttccgaaca
     1141 aatagggtaa gtgggaaagc ggcacaaggg ggcggcgcga aatgcggcat ccgccgcaat
     1201 cggcggcttt gcggaatgcc gcacgttgcc tcttgcaccg cccgaaatcc gtatgccgcc
     1261 gccgaaaatg ccgtctgaag gcacttcccc cttcagacgg cattgcctgc cgccgtattt
     1321 gcccgctatc cgcaatatcg gcagtccaat atgtctttgc ggatgtcgtt cagcaggcag
     1381 gctgccgcgt cttcgtgttt cgcctgtacg aagacaaaga gtcgggcgat gattttgccg
     1441 ataaagactt tttgcagggc gcaggcatcg gtcagggtgt gttcctgcag gaagctgcgg
     1501 atgtcttcag gcaaagtgta gtcgcgggcg acggcggcaa agcgggcatc ggtttgcagg
     1561 cggtcgagca gggtttggtt tttgtccaac ttcatgcctg cttctttttc ttcggcggtg
     1621 gcgcggacaa actggtcgta aatttcggca taaagcatat cgttcgggcc ttcaaaaatc
     1681 gtgaagggac ggatgtcgat ggcgatattg ccggcggtgt gtccgcgttc aaaacccttc
     1741 gcgcccaaga gtttttgcaa catttgcgcg gcggcgtaag tgtattccgt agcgagggtt
     1801 ttgacgatgt tcgcctccat cagctgatgg gcgacggggg caacaggcga aacggaatgg
     1861 cagacgtagc ggtaaagaat ctcggaaacc tgatggcggc gccggatttc gcggcgttcg
     1921 taatcgacga atctgatgtc gttgcggacg tatcgttcca ggttttcaag gatgtattcc
     1981 ataatgccgt gcgtcatgcc gatcagttgc aggcggctgc ggataaagat gttttggaac
     2041 gcgcgcaaac cggcagcgtc gctctgggag agtttcatca cggcggttgc aggcatttcg
     2101 gcatcgatgc ggttgacggc gtaacggacg gcgcgcaagc cttcggatgc gagggtttcg
     2161 cagcggatgt atgttttggg gacgagcagc aggtcgatga ctttggcgag tttgccgttt
     2221 ttgcgctctt tggcggcaac gaggaggaag tcgctttgcg agttgccctg ccagtatttc
     2281 gcggcgttga cgtaaatggt ttgtccgtcg gtatattcgt agtaagactg catttctcgg
     2341 gcaatcgccg cgccggaggt ttcgggttcg gtaacgccta aaccgccgcc ctcgcctttg
     2401 aaaaccatgt ccaaaccctg tgcgatttgc gcttcatcgc cgaactcttg cagtggctgc
     2461 aacaccagcg cgccttcgat gccggtacgc agcgtaacgg gcacgccgta atgccccgca
     2521 atccgcaaga cttcctgaat ttcaaactgg ctgcccttgc gcccgccgta ttttttgtcg
     2581 aggaagggca acagcaaacc cgcctgcttc aaggcaagcc atttgtcttc gggcaggtat
     2641 cgcatcaggt cgataccgtc tgcaaaaatg cggcggaatg cggattcgat gtgctttaaa
     2701 aaagcagccg tgtccatagt tgacggctgc gcgctcggtt cggtgtgtat catcggcttc
     2761 ctctgtcggt tcccattaat cggcggccgg tcaaaccgcc tgccacagtt tagagttgat
     2821 tttctaaact ttaccacaaa gtgcgccggg caacaatccg ccgacctttc agacggcatc
     2881 gcgccccctc ccgtgctaaa atgaccgttt gcatcactgt ccgccgattg ccgcactatg
     2941 acctacccca tccccaaacc ccgtgaaaaa tcccgttggc tcaatctttc gcaaggctcg
     3001 ctacccttag ctttggcgcg ttatctgccg cataagcagc tcaaggttgt cttgactcaa
     3061 gatgcggaac aggcgttgcg ccttcagacg gcatggcggt ttttccgtcc gcacgatacg
     3121 gcggtgtttc tgccggactg ggaaacgctg ccttacgaac gtttttcgcc gcatcaggac
     3181 ttggtgtcgg agcggctgtc ggcgttgtgg cagattaaaa gcggcgcggc ggatgtgttg
     3241 ttcgtgccgg ttgccacggc gatgcagaag ctgccgcctg tgccgtttct ggcagggcgc
     3301 acgttttggc tgaaaacagg gcagactttg gatataggcc gtctgaaaag cgatttggtg
     3361 gatgcaggtt ataaccatgt ttcccacgtt gtcgcggcag gcgagttcgc tgtgcgcggc
     3421 ggtattgtcg atttgttccc gatgggcagc gaaatgccgt accgcatcga tttgttcgac
     3481 gatgaaatcg acagcattaa aacctttgac accgaaacgc aacgcaccat ttcccccgtt
     3541 tccgaaatcc gcctgctgcc ggcacacgag ttccccaccg acagcgaggc gcaaaagatt
     3601 ttccgcagcc gcttccgaga agaagtcgac ggtaatccga acgatgcggc tgtatacaaa
     3661 gccgtcagca acggccattt cggtgcaggc gtagaatatt atctgccact gttttttgaa
     3721 aacgagctgg aaacgctgtt tgactatatc ggcgaagatg cgctctttgt ctctttgggc
     3781 gatgttcata ccgaggcaaa ccgcttttgg agcgacgtca aatcgcgtta cgccatggcg
     3841 cagggcgacg aaacctatcc gcctttgctt ccacagcatt tgtatctctc tgccgatgta
     3901 ttcgcaggcc gtctgaaaaa ctacggacag gtactgcccg atgtttccgg caaggaacac
     3961 accctgcccg accttgccgt caaccgacaa tccgacgagc ctttgcaggc attgaaggat
     4021 tttcagacgg cctttgacgg gcggattttg ctgtgcgccg aaagtttggg acggcgcgaa
     4081 accatgctcg gtttcttgca gcaaaacggt ttgaaagcca aacctgtgtc cgactggcag
     4141 ggcttcttgt cggcgcatga gccgctgatg attacagttg cgccgttggc atacgggttc
     4201 aaactgggcg gactgcaatc gccgaaccaa cagcaaacta cttccgcctc cgaaggagaa
     4261 ggcgatgcgg ttaccgacca aaccgaattt tccgcagccg caacaaaccc tctccctagc
     4321 cctctcccac aggagaggga acaaagtgca gccgtcgttt cagacagtct gaaagcagcc
     4381 gccatttcaa ccgaaagcag cctgcccctc ggtacaagta atctgcacgg gcaaatccga
     4441 cagcaacctg ccccttcccc tgagagagaa agtcgaggag aaagcaaagc agtttccgct
     4501 caaactgaat tttccgcagc cgcaataagc cctctcccta gccctctccc acaggagagg
     4561 gaacaaagtg ccgccgccgt ttcagacggc ctgaaaaccg aaagcagcct gtattccatc
     4621 gcaaataatc tgcacggaca aatccgacag caacctaccc cctcccccgt gggggaagat
     4681 tggggagaga gcaaaacagt tgccgctcaa accgaatttc ccgcatccgc aacaaaccct
     4741 ctccctagcc ctctcccaca ggagagggaa caaagtgcag ccgccgtttc agacgacctg
     4801 aaaaccaaaa gcagcctgta ccccatcgaa aatagtctgc acgggcaaat ccgacagcaa
     4861 cctgccccct cccccgtggg ggagggttgg ggagagggaa aagcagttgc cgaccaaagt
     4921 gccatcgccg tcattaccga atccgacctc taccaatacg tcgcccgttc gcgcgtccac
     4981 aaccgccgca agaaacacgc cgccgtttca gacggtctgt tgcgcgacct tgccgaaatc
     5041 aatatcggcg atcccgtcgt gcacgaagaa cacggcatcg ggcggtatat gggcttggta
     5101 acgatagatt tgggcggcga aaccaacgaa atgatgttgc tcgaatacgc aggagaagcg
     5161 cagctttatg tgcctgtttc gcaactgcat ttaatcagcc gctactccgg tcaggcgcat
     5221 gaaaacattg ccctgcacaa gctcggcagt ggcgcgtgga acaaggcgaa gcgcaaagcc
     5281 gccgaaaagg cgcgcgatac cgccgccgag ttgcttaacc tttacgcccg acgtgccgcc
     5341 caatcgggac acaagtttga aatcaacgag ttggattatc aggcgtttgc cgacggcttc
     5401 ggctacgaag aaaccgaaga ccaggcagcc gccatcgccg ccgtgattaa agatttgacg
     5461 caggcgaagc cgatggaccg cctcgtgtgc ggcgatgtcg gtttcggcaa aaccgaagtc
     5521 gccctgcgct ccgcgtttgt ggcggtaatg ggtggcaaac aagttgccgt actcgcaccg
     5581 accacgcttc tggtcgagca gcacgcgcaa aattttgccg accgcttcgc cgatttcccc
     5641 gtgaaagtcg ccagcctttc gcgtttcaac aacagcaaag ccaccaaagc cgcgctggaa
     5701 ggcatggcgg acggcacggt cgatattgtt atcggtacgc acaaactggt gcaggacgac
     5761 ataaaattca aaaacttagg tttagtgatt atcgacgaag aacaccgctt cggcgtgcgt
     5821 cagaaagagc agctcaaacg cctgcgcgcc aatgtcgaca tccttaccat gaccgctacg
     5881 ccgattccgc gtactttaag tatggcgctg gaaggactgc gcgacttctc gctgattacc
     5941 actgcgccca gccgccgcct cgccgtcaaa acctttgtca aaccgttcag cgaaggcagc
     6001 gtgcgcgaag ccgtattgcg cgaactcaaa cgcggcgggc aagtattttt cctgcacaat
     6061 gaagtggata ccatcgaaaa catgcgtgag cggctggaaa ccctgctgcc cgaagcccgc
     6121 atcggcgtgg cgcacggaca actgcgcgag cgcgagctgg aacaagtcat gcgcgacttt
     6181 ttgcagcaac gatttaacgt gttgctctgt tccaccatca tcgaaaccgg tatcgacatt
     6241 cccaacgcca acaccatcat catcaaccgt gccgacaaat tcggattggc gcagctgcac
     6301 cagcttcgcg ggcgcgtcgg ccgcagccac caccaagcct acgcctacct gctcacgccc
     6361 gaatacatca ccaaagacgc agaaaaacgt ctcgacgcca tcgcggcggc agacgaactc
     6421 ggcgcaggtt tcaccctcgc catgcaggat ttggaaatcc gcggcgcagg cgaaatcctc
     6481 ggcgaaggac aatccggcga aatgatacag gtcggcttca cgctctacac cgaaatgctc
     6541 aaacaagccg tgcgcgatct caaaaaaggc caccagcccg accttgacgc accgttgggc
     6601 atcaccaccg aaatcaaact gcacagcccc gccctactgc ccgaaagcta ctgccccgac
     6661 atccacgaac ggctcgtcct ctacaaacgc ctcgccgtct gcgaaaccgt gcaacaaatc
     6721 aacgccatac acgaagaact cgtcgaccgc ttcggactgc ccgaacaacc cgtcaaaacc
     6781 ctcatcgaaa gccaccactt acggcttatg gcaaaagaat tgggtatcaa tgccattgat
     6841 gcggccggcg aagcggtaac ggtaaccttt ggcaaaaaca acaatattga tccaaccgaa
     6901 atcatcctgc tgattcagaa cgacaaaaaa taccgccttg ccggcgccga taagctgcgg
     6961 tttaccgcag agatggaaaa tatcgaggtc agaatcaaca ccgtaaaaaa cgttttaaaa
     7021 accttgcaaa acagatgcct gcccaaataa agccgacacc gcaatgccgt ctgaaacacc
     7081 gttttccttg tccgaaagcc gccattatga atttgaagga aactccacta taatacggca
     7141 ttcagatttc cagacggcat cgcgcccgtc agaccgcaca caaaccaaaa ggaaatacat
     7201 gttccgtact atgcttggcg gaaaaatcca ccgcgccacc gtaaccgaag ccgatttgaa
     7261 ctacgtcggc agcattaccg tcgatcaaga tctgttagac gcggcaggca tctaccccaa
     7321 cgaaaaagtc gccatcgtca acaacaacaa cggcgaacgt tttgaaacct ataccatttc
     7381 aggtaaacgc ggcagcggcg tgatttgtct gaacggtgct gcggcaaggc tggtgcagaa
     7441 aggcgatatc gtcatcatca tgtcttacgt ccaactctcc gaacccgaaa tcgccgcaca
     7501 cgaacccaaa gtcgtcttgg tagacggaaa caacaaaatc cgcgacatca tctcctatga
     7561 gccgccgcac accgtgctgt aaccctgaaa acggacatcg attatggata ttaaaatcaa
     7621 cgacatcacc ctcggcaaca acttgccctt cgtcctattc ggcggcatca acgttttgga
     7681 aagcttggat tccaccctcc aaacctgcgc gcattacgtc gaagtaaccc gcaaactcgg
     7741 tattccctat atctttaaag cctctttcga caaggcaaac cgttcctcca tccattctta
     7801 tcgcggcgta ggcttggaag aaggcttaaa gatttttgaa aaagtcaaag cagagttcgg
     7861 catccccgtc attaccgacg tacacgaacc ccatcagtgc caacccgtcg ccgaagtgtg
     7921 cgatgtcatc cagcttcccg cctttcttgc gcggcagacc gatttggtgg ccgcaatggc
     7981 ggaaacgggc aatgtcatca acatcaaaaa accccagttc ctcagcccct ctcaaatgaa
     8041 aaacattgtg gaaaaattcc acgaagccga caacgggaaa ctgattttat gcgaacgcgg
     8101 cagcagcttc ggctacgaca acctcgttgt cgatatactc ggtttcggcg tgatgaaaca
     8161 aacctgcggc aacctgccgg ttattttcga cgttacccat tccctgcaaa cccgcgatgc
     8221 cggttctgcc gcatccggcg gtcgtcgcgc acaggctttg gatttggcac ttgcaggcat
     8281 ggcaacccgc cttgccggcc tgttcctcga atcgcacccc gatccgaaac tggcaaaatg
     8341 cgacggcccc agcgcgctgc cgctgcacct tttagaagat tttttaatcc gcatcaaagc
     8401 attggacgat ttaatcaaat cacaaccgat tttaacaatc gagtaacacg gtttcgcctt
     8461 atgatgcaga ctttccgaaa aatcagcctg tatgccgcaa ccttgtggct cggtatgcag
     8521 attatggcgg gttatatcgc cgcgccggtg ctgttcaaaa tgctgcccaa aatgcaggcg
     8581 ggcgaaattg ccggcgtatt gttcgacatc ctctcttgga gcgggcttgc cgtttggggc
     8641 gcggtactgg ctgccgcctt tgccgcccta acccggcggc aaaccgccct gctgcttttt
     8701 ttattgtccg cccttgccgc caaccaattt ttggttaccc ccgttatcga ggcactgaaa
     8761 tacgggcatg aaaattggct gttgtcgttt gtaggcggat ccttcggaat gtggcacggt
     8821 atttccagta ttgtttttat ggcggccgcc ctactttcga tttacttaag ttggcggctt
     8881 tccggcaaag atgccgtctg aagccctccc atttttttac ctcccttcac ttcacttgga
     8941 gaacattcat gagcgcaatc gttgatattt tcgcccgcga aattttggac tcacgcggca
     9001 accccacagt cgagtgtgat gtattgctcg aatccggcgt aatgggacgc gcagccgtac
     9061 cgagcggcgc gtccaccggt caaaaagagg ctttggaact tcgcgacggc gacaaatccc
     9121 gttattcggg caagggcgta ttgaaggcgg tcgaacacgt caacaaccaa atcgcccaag
     9181 ccctcattgg tatcgatgcc aacgagcaat cttatatcga ccaaatcatg atcgaattgg
     9241 acggtactga aaacaaaggc aatttgggtg cgaatgcgac tttggcggtt tctatggcgg
     9301 ttgcacgcgc cgctgccgaa gactcaggcc tgccgcttta ccgctacttg ggcggcgcag
     9361 gcccgatgtc cctgcccgta ccgatgatga acgtcatcaa cggcggcgaa cacgccaaca
     9421 acagcctgaa catccaagag tttatgatta tgcccgtcgg cgcaaaatct ttccgcgaag
     9481 cgttgcgctg cggtgcggaa attttccacg ccttgaaaaa actgtgcgac agcaaaggct
     9541 tcccgaccac agtcggcgac gaaggcggtt tcgcccccaa cctgaacagc cacaaagaag
     9601 ccctgcaact gatggtcgag gcgaccgaag ccgccggcta caaagcgggc gaagacgtat
     9661 tattcgcatt ggactgcgcg tccagcgagt tctacaaaga cggcaaatac cacttggaag
     9721 ccgaaggccg ctcctacacc aacgcggaat ttgccgaata tctggaaggc ctggtcaacg
     9781 agttccccat catctccatc gaagacggga tggatgaaaa cgactgggaa ggctggaaac
     9841 tgctgaccga aaaactgggc ggcaaagtcc aactcgttgg cgacgacctc ttcgttacca
     9901 acccgaaaat ccttgccgaa ggcattgaaa aaggcgtggc aaacgcacta ttggtcaaag
     9961 tcaaccaaat cggtactttg agtgaaaccc tgaaagccgt cgacttagcc aaacgcaacc
    10021 gctacgccag cgtaatgagc caccgctccg gcgaaaccga agacagcacc attgccgact
    10081 tggcagtcgc caccaactgt atgcagatca aaaccggttc tttgagccgt tccgaccgca
    10141 tggcgaaata caaccaactg ctgcgtatcg aggaagaatt ggcggaagcc gccgactacc
    10201 ccagcaaagc cgcattctac caactgggca aataaaaaag gttaaggtat gaagtgggta
    10261 actgtcgttt tatccttcgc acttgtctgt tgccaataca gcctctggtt cggcaaaggc
    10321 agcatcggac gcaacagcag tctgagagaa cagattgccg ttcaagaaga aaaaaaccag
    10381 acactcgccc tacgcaatca ttcccttgcc gccgaagtct atgatttgga aaacggtcaa
    10441 gaagccattt cggaaatcgc ccgggtagaa ctgggttata tccaagacgg tgaaaccttt
    10501 taccgactca tcaggcataa ccggtaatac cgtcaaaaag ccgtccgaac caatgttcgg
    10561 acggctttta tttcaacaaa ctgtcagaca gcccctcatc ctcccccgac aaaccgcaat
    10621 ccagcctgac atccccctcg acgcaacagc agcacggcag tatctcgtcc cgccccaaaa
    10681 aagccaaagg cggctcccga taagtaacgc ttccctccaa aatcttcact cggcacgatc
    10741 cgcaatatcc gcttcggcac tgatattcca ccatatgccc cgtccgttcc aagccttcca
    10801 acagggtctc gccctccaag agctcaaaaa aacccttatt cgtaccaatg cgcgccattt
    10861 ccgaccaatc aaaaatatag tggattaaat ttaaaccagt acggcgttgc ctcgccttgt
    10921 ccggattttt gttaatccac tataatccac tataaaagga acaataaccg atcctacccg
    10981 ctgtttttcc catcatacaa catacaaatg ccgtctgaaa catccggctt cagacggcat
    11041 tttttcaaaa aacgtttaca actcaaaatc gcccaaatca tccgtattca cttcagaatc
    11101 gatctgaccg atcaaataag aggatatttc cacttcctgc ggcgcgacct gtacgttatc
    11161 ggacgacagc cacgcattaa tccacggaat cgggttttga tttgcgcctt caaatccggc
    11221 cggcaacccc accgcctgca tacgcagatt ggtaatatat tcgacgtact gggataagat
    11281 ttctttgttc aaaccaatca tcgaaccgtc tttaaacaaa tatgccgccc attctttttc
    11341 ctgttccgcc gcttttttga agagttggaa acattcgtcc tgcaactcag cggcaatttc
    11401 tgccatttca gaatcatcga cacccgaacg catcagatta agcatatgct gcgtgccggt
    11461 caggtgcagg gcttcgtcgc gggcaatcag tttgatgatt ttggcgttgc cttccatcaa
    11521 ctcgcgctcg gcaaaagcaa acgagcaggc gaatgaaacg tagaaacgga tggcttccaa
    11581 cacgttgacg cacatcaggc agagatagag ttttttcttc aacccgcgca aagacacagt
    11641 aatgggtttt ccgccgacat tgtgcacccc ttcgcccaac agattgtaat actgggtgta
    11701 ttcgattaag tcgtcgtaat agcaggcaat gtcttcggcg cgggcggtaa tgtattcgtt
    11761 ttcgacaata tcatcaaaca cgaccgacgg atcattcaca atattgcgga tgatgtgggt
    11821 atagctgcgc gagtgtatgg tttcgctgaa gctccacgtt tcaatccacg tttccaactc
    11881 gggaatcgaa accaaaggca gcaaggcaac attcggactg cgcccttgga tggaatcgag
    11941 cagtgtttgg tatttcagat tgctgatgaa aatatgtttt tcgtgttctg gcaggttggc
    12001 gtagtcgata cggtcgcgcg acacgtcgat ttcttccggc cgccaaaaaa acgacaattg
    12061 tttttcaatc agtttttcaa atacctcgta tttctgctgg tcataacggg cgacattaac
    12121 cggctgaccg aaaaacatcg gctcattcag cgcgtcgttt ttggttttgg gaaaggtgct
    12181 gtatgacata gttaagtgtt cgcaggacat aatctattct ctatattaat taaaaatgat
    12241 ctgtaagttt taactaacct gctgagactt tatatattta ataaaatttt ctaaagttaa
    12301 aacatgggtt ttcccaaaag ttccaaatgt ttttacggat tctttaggga caactaaggt
    12361 taaattctca tcactcattt cctgaatttg agtatcagat ataccttctt gtagagtaaa
    12421 taaatgctta tgaggaattc gatctgcttc atttaaaact tggcgccagc gatccttaca
    12481 agtagtcttt gaacctagca ttgtcagctt ttcagtttga aatatgaaat tccctgattc
    12541 atccatcgca tggtactctt cacttccagg aaataaaaaa tctggtttct ttttcccttc
    12601 tgtcttcgct tgcgtctcaa attttaagga aaattcgtca aatattcttg ataaatgtaa
    12661 ctctaaagat tttcctgctc ttgcttttct gcgatttgta aaagaatgtg caaaattaat
    12721 gaagctatcc aaatcagtaa tttttgattt aataatttca aattcacgtt tttcaaaaat
    12781 ggaaaataat tcatattcct ttgaaaccca ttgttttaac acattactaa cgttagtttt
    12841 atttaatgca atattttccc ttgccaaaac tgccatttct tcagttttgg ggaagttagg
    12901 aaatagtgaa aataattttg ttaggttatc ctctacttct tgttttttag gtaaatataa
    12961 acttcctggt aaaatgttag tttctgcgat aaatatttca atatcttcat ctgaagataa
    13021 aacaaaagca tggaataata aatctttata acaagctctg cacaatacca ataagaaacc
    13081 actatattta tcacttaaaa attcaaaatt cttgccaaaa cccgtaattc tcgcctcatt
    13141 tctcgtgcct tggccgtaat atttaaaatt gctattagtg acgcttccat cttgccaatt
    13201 aattttgatt gaaatagttt tatttgttcc cttttggcaa actacatcaa agaggtcgtc
    13261 tgaaaaatgt ttttgaatat aaaatcctga ttggtgacta ccagtagtac caacatcatt
    13321 gggacgaata taacgacagt atactgcaat tgatttattt gctgtctgta ttgctgaagc
    13381 tactaagtca tcggatttat ttactacttg tattgtctga gctgccaagt cattcatttt
    13441 ttaactcatt tataatttgt ttagcaatag cttgaaccaa cggtacagca attgaattgc
    13501 caaactgctt gtatgcagct gtcttggata ctgcatcaat aacaaaatct ttaggaaatc
    13561 ccattaaacg cgagcactcc ctaggtgtca gcttcctagg attttttcct ttctgaggga
    13621 tgagtatttc ggaaccatct ttgtaatatc gtgcagatag agttcgtgat attccatcta
    13681 aatcaactaa tccaaaacca aatccattac cctttgcctt atgtttttta gcgtaatttt
    13741 gaaggtaaag ccataagtta tcagaaagag taaaagaatt atctacatca tcttccaaaa
    13801 tttgctttaa ttttggttgt gattctggtg gggaaggaaa attgaaattt atttccttat
    13861 taaaatattg tctatcaaaa cctacaataa aaatacgctc cctattttga ggaacataat
    13921 attttgcatt cataacttga taaaatatct gatagtcaag ctcttctaaa gtccctttaa
    13981 ttactttaaa tgtatttcct ttgtcatgcg aaacaaggtt tttcacattc tctaaaagaa
    14041 aaattttagg tcgatgtttt ccaataattt cagcaacatc aaaaaataga gttccctgcg
    14101 ccttatctaa gaagcctgtt tctcgtccta ggcttttttt ctttgaaaca ccagctatag
    14161 agaatggctg acacgggaat cctgctgtta atacatcaaa cttacttgga atagctgctt
    14221 tggtttcctt taatgtaata tctccataag gaatatcatt aaaatttact tggtaggttt
    14281 gacgggcttt atcatcccat tcactagaaa atacacatcg cccaccaaca ttctccattg
    14341 caatgcgaaa accacctatt cccgcaaata aatcaataaa agtaaatttc tcattattta
    14401 aggtaactat attatctaat tgattttgta ttttattttt catattttat atttcagatg
    14461 atttattaat ctaaaggcca tctgaaaact ccccctttca tcaaatctta caagccccgc
    14521 ccgcgcagcc gtcatcctga atatcggtct gcgtatcgtc cgcgccgtcg cgggtgttat
    14581 ggtagtacag ggttttgacg ccgtatttgt aggcggtcag caggtctttg agcatttgtt
    14641 tcatagaaac tttgccgcct tcgaatttgc ccgggtcgta ggcggtgttg gcggaaatcg
    14701 cctgatcgac gaatttttgc atcacgccaa caagtttcag atagccttcg ttgccgggaa
    14761 gctgccacag ggtttcatag gcatttttca gggtttcaaa ctccggcacg acttgtttca
    14821 aaatgccgtc tttcgatgct ttgaccgtta ccaatccgcg cggcggctcg ataccgttgg
    14881 tggcgttggc gatttgcgaa ctggtttcag acggcatgag tgcggttaga gtagagttac
    14941 gcaggccgta tttgacgatg tcggcacgca ggctttccca atcgtagtgc aaaggctcgt
    15001 tacacacagc gtccaagtct tttttgtagg tgtcgatggg cagtttgcct tgcgaataaa
    15061 cggtttggtt aaagagcgtg cacgcaccgt attctttggc aaggtttgcc gatgctttga
    15121 gcaggtaata ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg gagccgtcgc
    15181 tgtagcggac accgtttttc gccaaataat acgcatagtt gatcacgccg ataccgagcg
    15241 aacggcggcc catagtggag gtacgcgcgg cttctaccgg atatccctga taatctaaaa
    15301 gtgcatcgag cgcgcgcacg gtcaaatcgg caagcccttc caattcgtcc aagctgttta
    15361 atgcccccaa gttgaaggca gacagggtac acagggcgat ttcgccgttc gggtcgttga
    15421 tattgtccaa aggtttggtc ggcagcgcga tttccataca caagttggac tgatgaacag
    15481 gcgcaacgcg cggatcgaac gggctgtgcg tattgcagtg atcgacgttt tgaatgtaga
    15541 tgcgcccggt tccggcacgc tcctgcatca gcgtggaaaa caggtcggca gccggaatga
    15601 tgcgcttgcg gatatcaggg tcttgctcgt atttcgtata gagccgctca aattcgtctt
    15661 ggtcggcaaa aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac agcgtaatgt
    15721 tgccgccctt aatcaggcgg gtgtacagca ggcggttgat ttgcacgccg taatcaagct
    15781 gacggatacg gttgtcttcc acgccccggt tgtttttcaa caccagcagg ctttcggctt
    15841 cgatatgcca caaggggtag aacaaggttg ccgcgccgcc gcgcacgcca ccttgcgaac
    15901 aggatttgac tgccgcctga aacattttaa agaagggaat gcagccggta tgccgtgctt
    15961 cgccgccccg gatttcgctg tccagtccgc ggatacgtcc ggcattgatg ccgatgcccg
    16021 cacgctggga aacgtatttc acaatcgcgc tggtagtggc gttgatggaa tccaggctgt
    16081 cgtcgcattc gatcaatacg cagcttgaga actggcgcgt aggcgtacgc acgccgctca
    16141 taatcggggt cggcagcgat actttaaatg tggaaacggc atcgtaaaac cgtttgacgt
    16201 aatcgaggcg tgtttctttg ggatatttgc tgaaaagaca catcgccacc agaacatata
    16261 agaactgcgg tgtttcataa atctggcggg taacgcggtt ctgtaccaga tatttgcctt
    16321 cgagctgttt gacagcggca taggaaaagg acatatcgcg ttcgtggtcg atataggcgt
    16381 tcagctcgtc aaattcttcg cggctgtaat cctcaaggat atgcctgtcg tattttgcgg
    16441 catcggtaag ttttttaacg tggtcgtaaa ggtgcggcgg ctcgtactcg ccgtaggcta
    16501 ttttacgcag atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc ggggtatctt
    16561 ccgaaattaa atcggcagcg gctttgatga tggtttcgtg gatgtcgtcg gtgcggatgc
    16621 cgttgtagaa ctggatgtgc gatttcaact cgacctgaga tacggaaaca ttttccaatc
    16681 cgtccgccgc ccaagtgacg acacggtgaa tcttatccaa atcaatggct tctaatcttc
    16741 cgtctcgttt ggttactttc aaatcagtcg gtgtattcat cgcttcctct tccactcttg
    16801 atattcaaga cacagtcttt tcaaataaat taaggcagac aatatagtgg atttttggca
    16861 ttttctccag tcttgacaac ggttgtattt ttcagtttca ggcaatgcgc gataaaaccc
    16921 gcctgtcgta tttttcctat aatatttgtt ttatctgata attctttacc gataaaaaac
    16981 gggtaaattt tttgcctttt gaccggctcc ggctacaagg cggcgaaata aggattttcc
    17041 gacgaaaaag gaaagcttcc tgttttctgc ccctgcaaat tgttaaattt tgcaaagtat
    17101 gattttgccg cgccgccgcc gacaaattcc attttcttac cgattggaat ttattattga
    17161 gattaatgtg ttatttgaat ccgcatatca aacggcaagt tttgtcggct gaatgagtct
    17221 gaaactgccg acaatttgcc cgttccatcc ttccatccta tactggaagg aatgacaaac
    17281 tgaaaggatc gtcatatctg tcaaaaagat gcttgccata ctgttgtctg caatattggg
    17341 actggtatcg acaactgccg ctgccggtac gtcagaaccc gcccaccgcg ataccaaaca
    17401 tatccgcaag gcaaacaagc agatgctgca ccccgaatgc aggaaatatt tggaacgccg
    17461 tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca aaaaggcgcg
    17521 caaagcattc cgctccctgc cttataagga acagaaaacc caatgccggg cggcttatga
    17581 ggctttcgat gatttcgacg gcagcaggtt ccgccgttaa tcccatataa aatatgccgt
    17641 ctgaacacag gttcagacgg catattttat gaatacaaca aactatcctt acgccatttc
    17701 gtttgaggtc ctcccgttgc cgcagcctta atatagtgga ttaacaaaaa tcaggacaag
    17761 gcgacgaagc cgcagacagt acaga
//
LOCUS       DAWKQB010000029        17728 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.029, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000029 DAWKQB010000000
VERSION     DAWKQB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.029"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            200..1876
                     /gene="ettA"
                     /locus_tag="WJL14_000726"
     CDS             200..1876
                     /gene="ettA"
                     /locus_tag="WJL14_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ7655827.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAKGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
     gene            complement(1936..2421)
                     /locus_tag="WJL14_000727"
     CDS             complement(1936..2421)
                     /locus_tag="WJL14_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ7655828.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMLGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(2414..3370)
                     /gene="thiL"
                     /locus_tag="WJL14_000728"
     CDS             complement(2414..3370)
                     /gene="thiL"
                     /locus_tag="WJL14_000728"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ7655829.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFANVKPEDLAWKVLAVNVSDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            complement(4191..4919)
                     /gene="rmpM"
                     /locus_tag="WJL14_000729"
     CDS             complement(4191..4919)
                     /gene="rmpM"
                     /locus_tag="WJL14_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ7655830.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            5286..6236
                     /locus_tag="WJL14_000730"
     CDS             5286..6236
                     /locus_tag="WJL14_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ7655831.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNTGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNLDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(6349..7083)
                     /gene="fnr"
                     /locus_tag="WJL14_000731"
     CDS             complement(6349..7083)
                     /gene="fnr"
                     /locus_tag="WJL14_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ7655832.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            7284..8705
                     /gene="hemN"
                     /locus_tag="WJL14_000732"
     CDS             7284..8705
                     /gene="hemN"
                     /locus_tag="WJL14_000732"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ7655833.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(9058..10632)
                     /locus_tag="WJL14_000733"
     CDS             complement(9058..10632)
                     /locus_tag="WJL14_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ7655834.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            10957..12057
                     /locus_tag="WJL14_000734"
     CDS             10957..12057
                     /locus_tag="WJL14_000734"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ7655835.1"
                     /translation="METQLYIGIMSGTSMDGADAVLMRMDGGKWLGAEGHAFTPYPGR
                     LRRKLLDLQDTGADELHRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHSYSVQLADLPLLAERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WMAACWVNRIPGSPHKATGASKPCILGAGYYY"
     gene            12177..13118
                     /gene="mafA"
                     /locus_tag="WJL14_000735"
     CDS             12177..13118
                     /gene="mafA"
                     /locus_tag="WJL14_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7655836.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKKPDVGNEVIRRRKGG"
     gene            13122..14618
                     /gene="mafB"
                     /locus_tag="WJL14_000736"
     CDS             13122..14618
                     /gene="mafB"
                     /locus_tag="WJL14_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ7655837.1"
                     /translation="MKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHRMGNLLIQQANINGTIGYHTRFSGHG
                     YEEHAPFDNHAADSASEEKGNVDEDFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTHSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AVIGGLGSVAGFEKNTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKA
                     AVSGDFSAAYNTRTTRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSI
                     QHILYGDEAGGGHLFPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKY
                     IAKGRPARWVSYETRDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            14633..14863
                     /locus_tag="WJL14_000737"
     CDS             14633..14863
                     /locus_tag="WJL14_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7655838.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            15012..15680
                     /locus_tag="WJL14_000738"
     CDS             15012..15680
                     /locus_tag="WJL14_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7655839.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYR
                     ETVYIKVSDGIANSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNAA
                     EKWLGENYKSYPNGRYVSQDGMRQVRYGYHETNSSTHHGHFESYDKPNGRVIENSAVT
                     IIRD"
     gene            15684..15956
                     /locus_tag="WJL14_000739"
     CDS             15684..15956
                     /locus_tag="WJL14_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655840.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            16077..16622
                     /locus_tag="WJL14_000740"
     CDS             16077..16622
                     /locus_tag="WJL14_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ7655841.1"
                     /translation="MITVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVDNLSDMNRKPFAW"
     gene            16758..17432
                     /locus_tag="WJL14_000741"
     CDS             16758..17432
                     /locus_tag="WJL14_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ7655842.1"
                     /translation="MVRTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTTRYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNECPTKPKPTNHAQQRKEEAKNDSH
                     RSVGDSNRVVREGKQYLDADTGNHVYVKGEKVVILTPDGRQVTQFKNSKANTSKRVKN
                     GKWTPK"
     gene            17410..17691
                     /locus_tag="WJL14_000742"
     CDS             17410..17691
                     /locus_tag="WJL14_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655843.1"
                     /translation="MENGHRNNRSPLEKRIFYLEHSGQYLMICALSDYSKNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
BASE COUNT         4889 a         4819 c         4158 g         3862 t
ORIGIN      
        1 ctttggaaaa tccgaaatgg ttgccggggc ggcgatcccc tatcattatt atttttttgt
       61 ctatataatt tcaaagggat aagcggattt tatgaatcct gcccgatttt ggcaataccg
      121 gttcgcggat aaactggctt aaatcaaatt atcggttaaa atggccgtct gaaatttgtt
      181 tgatgaaaac gagaaaacca tgtcccaaca atacgtctat tctatgctgc gcgtgagcaa
      241 ggttgtgccg ccgcagaaaa ccatcattaa agatatttcc ctttctttct tccccggcgc
      301 gaaaatcggc ctgctcggtt tgaacggcgc gggcaagtcc accgtgctgc ggattatggc
      361 gggtgtggat aaggaatttg agggcgaagc cgtgccgatg ggcggcatta aaatcggcta
      421 cctgccgcaa gagcctgagc ttgatcctga aaaaaccgtg cgcgaggaag tggaaagcgg
      481 tttgggcgaa gtggctgccg cgcagaaacg tttggaagaa gtgtatgccg agtacgccaa
      541 tcctgatgcg gattttgacg cgttggcaga agagcagggc cgcttggaag cgattattgc
      601 ggcaggttcg tccacgggcg gcggtgcgga acacgaattg gaaatcgccg ccgacgcgct
      661 gcgcctgccg gaatgggatg ccaaaatcga taatttgtcc ggcggtgaaa aacgccgcgt
      721 tgccttgtgc aaactcttgt tgagcaaacc cgatatgctt ttgctggacg agccgaccaa
      781 ccacttggac gcggaatctg tcgagtggct ggagcaattt ctcgtgcgct tccccggtac
      841 agtcgttgcg gtaacacacg accgctactt cctcgacaac gccgccgaat ggattttgga
      901 actcgaccgc ggccatggta ttccgtggaa aggcaattac tcgtcttggc tggagcagaa
      961 agaaaaacgc ttggaaaacg aggcaaaatc cgaagccgcg cgcgtgaagg cgatgaagca
     1021 ggaattggaa tgggtgcgcc aaaatgccaa aggccgccaa gccaagtcca aagcgcgttt
     1081 ggctcgtttt gaagaaatga gcaactacga ataccaaaaa cgcaatgaaa cgcaggaaat
     1141 cttcattccc gtcgccgagc gtttgggtaa cgaagtgatt gaatttgtga atgtttccaa
     1201 atcgttcggc gacaaagtgc tgattgacga tttgagcttc aaagtgcctg cgggcgcgat
     1261 tgtcggcatc atcggtccga acggcgcggg taaatcgaca ctgtttaaaa tgattgcggg
     1321 caaagagcag cccgattccg gtgaagtgaa aatcgggcaa accgtgaaaa tgagcttgat
     1381 tgaccaaagc cgcgaaggtt tgcaaaacga caaaaccgtg ttcgacaaca ttgccaaagg
     1441 tcgcgatatt ttacaggtcg ggcagtttga aatccccgcc cgccaatatt tgggacgctt
     1501 caatttcaaa ggcagcgacc aaagcaaaat cacggggcag ctttccggcg gcgaacgcgg
     1561 acgtttgcac ttggcaaaaa ccttgttggg cggtggcaat gtgttgctgc tggacgaacc
     1621 gtccaacgac ctcgacgtgg aaaccctgcg cgcgttggaa gacgcattgc tggaatttgc
     1681 cggcagcgtg atggtgattt cgcacgaccg ctggttcctc gaccgtattg ctacgcatat
     1741 cttggcttgc gaaggcgact ccaaatgggt gttctttgac ggcaactatc aggaatacga
     1801 agccgacaag aaacgccgac tcggcgaaga aggcacgaaa ccgaaacgca tcaaatacaa
     1861 accggtaacg cgttaacctc cgaaacaatg ccgtctgaaa ggctttcaga cggcattttt
     1921 acaaggcagc accgtttaaa acagcattgc aatcctcaag acaatcaaag tcatcaccgc
     1981 agccgccata tcgtccgcca taatgcccaa accgccgtgc agattcttgt caaaccaacc
     2041 gacgggagac ggtttgagcg cgtcaaacag acggaatagg acaaatgccg ccagccacca
     2101 cgtccacctg aacggcacaa acgccagcac aaacagcatg gcgacaatct cgtcccaaac
     2161 aatcccacca tggtcgctga cacccgtttc acgttccgca taagcgcaaa tgcgtatgcc
     2221 caacataaac agcacgatac acaaaaaagc cagcagcagc ccgtctatgc cgagcaaaat
     2281 cagcacaaat gccaaaggca gtgccgccaa agtgccaaat gtacccggcg cgaacggagc
     2341 cagcccgctg ccgaaaccaa aagccaaaaa acacaacggt cgtttcaaca gccacgcaaa
     2401 gtcaggttta aaatcagcca aaatgatcga atcctaaaga atgtagttcc aattccctgc
     2461 cgtcggcatc taaaaccttc agacggcatc ctccgttgat tttgccgatg cgcgttaccg
     2521 gcacgccgca ccgttctgcc gcatcaagca cgcggctgcg gcaactttcc ggcgcggtaa
     2581 acaccagctc gtaatcgtcg ccgcccgcca aagtataaga cagccattgc gctcggggca
     2641 aaatatcttt caatacggat aaagacggca gcgaatcggc ccaaatttcc gcccccacgc
     2701 cggaagcggt caggatatgc cccaaatctt gcgcgaggcc gtctgaaaca tcctgcgccg
     2761 cccgggcaaa cggcaacagc gcaagcccca gcccaaccct tggctcgggg cggagcagct
     2821 tttgttcgca ttcggcaaac acatcatccg gcaacacaca ccgtttcaga cggcagttca
     2881 aagccgccgc cgccataccg acacgccccg acacccaaat atcgtcgccc gcaaccgccg
     2941 catcacgccg caacgcccta cccttcggca attcgccgat aatggttaca ttgaacgcca
     3001 tatcgccctt ggtcgtatcg ccgccgatta acgttacgcc aaactttttt gccaaaccga
     3061 aaaagctgcc gcaaaaccgt ttcagccata cctcatccaa ttcgggcaac gccgcgctca
     3121 gcaacaccca acgcggtatc gcacccatcg ccgccatatc cgaaacattg acggccaaaa
     3181 ccttccaagc caagtcttca ggtttgacat ttgcaaaaaa atgcctgtcc ttcaaaagca
     3241 tatccgcact gaaacacaaa tcgaagcctt cacgcgggcg gacaatcgcc gcatcgtcgc
     3301 ctatgcccaa tacgacatca ttatccgtgc ctgtttgcaa ataccgtttg atgaagtcaa
     3361 attctttcat atcccaaaat atccggcggg cgaatccaat ttaaatcata gcaaggcagc
     3421 aaaggccgta caaacaataa agcggctcat tcagtgtttc agcgtatttc acttttagct
     3481 ttggccatac cgtacccgta tgcaccgacc accctgtttc aacacacaaa cggcatatca
     3541 aagcaccgca ctgcacgcca ccccgaatgc aaagcggcaa cttccgccac cgattaggct
     3601 caatcgctgc aactggcaag gtttcaaccg aaaagggctg atgataaaac catatccggc
     3661 atacagtgcc gcacacatct ggcacgattc catccatttc ccaatgaagg ggaaaggcgt
     3721 gaatttgtat gaattatccc tttcagggct tgatacagaa aggattccct ttctatgggc
     3781 aaacccacat cctaccgcct taaaatcaga gtttcaaccg atatagtgga ttaaatttaa
     3841 accagtacag cgttgcctta ccttagctca aagagaacga ttctctaagg tgctgaagca
     3901 ccgagtgaat cggttccgta ctatttgtac tgtctgcggc tccgtcgcct tgtcctgatt
     3961 tttgttaatc cactataaag cacggcaatg ggacacaaac ctgaatctga atctgaacct
     4021 gcaccccgat tctgacgata gccgtttcag aaaaccaccc aaaaaaacaa accccgcaaa
     4081 agcgggggtt tgtttcacat catcgatgcc gaccgacgtt tcacgaatat tgcaacagta
     4141 cctggtacaa aatccactaa cctcatgcat cggcaagata ttgcctagcc ttagtgttgg
     4201 tgatgattgt gtgccggcac aacctgacgg gttacgatgc tgcggatttt cacatccaca
     4261 cggcggtcag gttcgataca tgcaatcaga gcctcacgtt ttttggcttt agagactttc
     4321 gcacccagtt tggcaacttc ggcttcacaa acttgagtca tttgcgcttg agattcgccc
     4381 aagccgacag cagaaattct agaaacaggt acgccgttgc tgaccaggtt gtttgccact
     4441 acgtatgcgc ggcgttcgga cagggcctga ttgtatttgt cagaacccat aaagtcggta
     4501 tggccttcga cgcggacaga ttggatattg gtttgaccca ggcgttgcgc caatactttc
     4561 aggttgtctt gagcttcggc gcgcaatgaa tccttatcga aaccgaacag ggttttggca
     4621 gacagggaaa tggtttcatc aacatattgc ggagcctgct ccacaacgac gacaggcgcg
     4681 ggtgcgggtt cgggttctgg ctcgggttcg ggggcagcaa ccgcatcgcc gcattctacg
     4741 cgaccttggc ttgctttatc aaagtaggcg tttttccagc attcgccata gttgttgcgt
     4801 acgatttcgt tcgactggcc gcttacggtg taaccctgaa cggacgcctc gcccgcaaca
     4861 gcagtgccgg aagcgagcaa tgcaacgaat aatgcgctta atttcagctg tttggtcatt
     4921 ttattactcc ctcattaaat ttgtacagca gatacagaag caactgctga attgccaaca
     4981 tacaggccgc tgtagaaaca tggcggcaaa tcggatattt cagtctccac tataaagaag
     5041 cagggggagg actgtcaata ctcaacgcct gaaaacaacc gtaaaacagc ctgtttcctg
     5101 atagtctatc atgccgaaaa atcaattccg tgtcattact ttagggggat tttttccata
     5161 tcgcacgatt gccgttgcac aaaaacaaca aacacaacgg caaagcccca taccgtaccg
     5221 gcaagaaaat atgataaatt ataaacaatg ttcagccacc cgacacagac ccacaccgac
     5281 ccgccatgaa attacaacaa ttgaaatacg ccttagaagt ttaccggcac aacctgaacg
     5341 tttccgaagc ggccgaagcc ttgtttactt cgcaacccgg catctccaaa caaatcaaat
     5401 tgctggaaga agaaatcggc attcagattt ttatccgcag cggcaagcgc gtggtttcgg
     5461 tctcgcagcc gggcaaggtg gttttggata ttgcggaacg tattttacgc gatgtacaga
     5521 acattaaaaa tatcggcagc gagtttaccg gacaggacag cggttcgctg acggttgcca
     5581 cgacgcatac tcaggcacgc tatgccctac ccttgattgt tgccgatttt gtgaaacgct
     5641 atccgaaagt caacctgacc atcaaacagg ggagccctgc cgccatcgca cgtatggtta
     5701 cctcaggcga ggcggacttg gcgattgtta cggaacggat agacgaccat cccgaactgg
     5761 gaaaacttcc ctgctatgac tggactcatg cggttatcgt accgaacgac caccccttgc
     5821 tcgaatgcag aaaccccctc cgtattgaag atttggcgag gtttccacta attacctatg
     5881 aatttgcatt caacacgggc agcagcatcg cgcgggcatt ttccaaagcc cgtttggaac
     5941 agccggatgt cgcattggcg gcggcggata cggacgtatt gaagacttat gtgcgcttgg
     6001 gtttgggcgt gggactgatg gcgaaaatgg cgtacaacct ggatacggac ggcgatttgc
     6061 agcttgtgga tgcggcacac ctgttcgagc cgtcgccgac gtggattgct ttgcgcagcg
     6121 atacttattt gcgcggatat gcctacgact ttatccaagc gtttgcgccg cacctgacac
     6181 gcgagaaggt ggataggatt ctgtacacgc ccatcagcga ggatttttcg atttaggcgg
     6241 ctgccggttt tcagacggca ctttgcggca gatacaacaa acaggacaga tgttttcgtc
     6301 tgccctgtgt ttattgagaa tgccgtctga aatgttcgta cgggttaatc aaatggcgtg
     6361 cgaacaaccg gataccatct tcttcaacac ctgcagattg aggatcttga tgtgtttgtg
     6421 ttcgacggaa atcaatcctt cctgatggaa cttggataat gtgcggctga cggtttcaag
     6481 tttcagcccg agataactgc cgatttcttc gcgggacatt cttaagatga agtcgttggc
     6541 agcaaaacct cgggaataaa ggcgttggga aaggttcagc aggaaggcgg caatccgctc
     6601 ttcggcgcgc atattgccca acagcagcat aacaccttgg tcgcgcacga tttcacggct
     6661 catcatgcgg aagaagtgcg tacgcaggct ggggatgttt tgccccagtt cttcgatgtg
     6721 ggtaaacggc agttcgcaca cttcgctgtc ttccaaggcg accgcgtcgc aactgtgcac
     6781 atgggaacag atgccgtcca tgccgatgag ttcgcccgac ataaagaaac ccgttacctg
     6841 atcgcggccg tcctgactgg cgacggttgt tttgaagaag cccgaacgga tggcaaagag
     6901 cgaggtaaag gcttcgccga cacagaacag gtattcgccc tttttcaggc ggcggctttg
     6961 acggatgacg gcatcgagtt ggctgagctc gttgggcagc agcccgacag gcaggcagag
     7021 ttcccgcaaa gaacaggaag aacacagcgt tttcatctga tgtgtagtat tatgcgaagc
     7081 cataccgtac ctttttgtgc gctttgcccc atcatgatta tagtggatta aatttaaacc
     7141 agtacggcgt tgcctcgcct tgtccttatt taaatttaat ccactatatg tgcttattga
     7201 cacatatcaa gacaggttta tcatactgtg gcattctacc aaactatcca aaatttgact
     7261 aacatcataa aaccacacac accatgaaaa tcattcagat acagaacaat cacaatgtaa
     7321 acgatgaccg tcccgagttt gaccgcgcgc tgattgccag cctgcccgcc agcggcccgc
     7381 gctacacttc ctaccctacc gccgaccgtt tccatgacgg tttccgcgaa ggcgaatata
     7441 tcaaagcttt acatttgcgc ggtatgggcg cgttaaacaa accgctttcc ctttacattc
     7501 acattccgtt ctgcaacacc atctgctact actgcggctg caacaaaatc atcaccaaag
     7561 acaaaagccg cgccgatgcc tacatcgaat atcttgaaaa agaaatggaa ctgctcgctc
     7621 cacatctgaa cggacggcac cagcttgccc aactgcactt cggcggcggc actccgacct
     7681 ttttgagcga cgaacagatc gaacgtgtct tccgcatgat acgcaaacat ttcgagttaa
     7741 tccccaccgg cgaatactcc atcgaaatcg acccgcgcaa agtcagccgc gacaccgtcc
     7801 tcatgctcgg cagactcggc ttcaaccgca tgagcatcgg cattcaggat ttcgacccca
     7861 aagtgcaggc ggcggtcaac cgcatccaaa gttacgaaga aaccaaagaa gtcatcgatg
     7921 cggcgcgcga agcggggttc aaatccgtca gcgtcgattt gatttacggc ctgccgcacc
     7981 agacttcgga aagcatcaaa accaccatcg ataccgtttt gtcgctcgat cccgaccgcc
     8041 tcgcccttta tcactacgcc cacctgccgc acgtgttcaa accgcaacgc cgcatcgata
     8101 ccgcctccgt ccccgacagc gaagagaagc tcgatatgct gcaatactgc gtccaaaccc
     8161 taaccgaacg cggctacgtc ttcatcggca tggatcattt cgccaaacct gacgacgaac
     8221 tctccatcgc cctcaaagaa ggcttcctcc agcgcaactt ccaaggctat tcgacctacg
     8281 cggattgcga tttggtcgcc atcggcgtgt cgtccatcgg caaaatcggc agcacctatt
     8341 cccaaaacga acgcgacatc gatgcctact atgccgccat cgacgaaggc agactgccca
     8401 tcatgcgcgg ttatcagctc aatcaggacg acatcctgcg ccgcaacatc attcaggatt
     8461 tgatgtgccg tttcgcgctc gactatcgga tttacgaaag tatgttcggc atcccgttcg
     8521 accgctactt caaagacgaa ctggcggatt tggaaaaact cgccggtttg ggattggtgc
     8581 gcctgaacag ccacggactg accgttaccc cgaaaggacg cttcctcatc cgcaacatcg
     8641 ccatggtttt cgattaccac ctgcgccata aagaaaccaa ggcgaaatat tcgcaaacgg
     8701 tgtgattgtg gctaacgtac aaatgccgtc tgaaaggctt tttcagacgg cattttgctg
     8761 ccggcaggat aagtgttttc aagaacaggc ggcggcatat cataacgttc cgcacctttg
     8821 tgtccgaccg ttccgaaacc aagatatagt ggattaacaa aaaccagtac agcgttgcct
     8881 cgccttgctg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     8941 tcactatacc aaacggcata tcccgacaga acagattgtg cataaggcac aagcccgcac
     9001 attccatcaa caaaatgccg tctgaacacg ggttcagacg gcatcagtat tttacaatca
     9061 gaatactgcc tgtaaaacca agtagcagac aaccgacaat acggcggcgg caggcagggt
     9121 aatgacccac gccaaaccga tgggcttcat cagtttccag ttggcattgc ggttgaccag
     9181 accgatgccg agtaccgcgc cgaccaagat atgcgtactg gacacgggca gccccatcag
     9241 cgacgcgccc atcacgacgg atgcggcgga cagttcggcg gtaaaacccg aagcaggatg
     9301 catttccgcc aaactcgtac cgacggtttt aatcacctct ttaccgacaa accacaaacc
     9361 gacaatcagc gcgataccga aagtcagcat cgcaatcggg gggacgacat tttgcgcggc
     9421 aacgctgttg gtacgcaaaa catccataat cgcggcaaac ggaccgatgg cgttggcgat
     9481 atcgttcgcc ccgtggctga atgcgaagcc gcaggcggta aagacctgca tccatgaaaa
     9541 catctgaaag gttgatttgc ccaagtcttt acgcttgagg cttttggcaa acacaaacgt
     9601 ccccatccac accgccgcgc ctatcataaa gatggtcagg aagctgttga cgttgctcat
     9661 ccccaaatgc aggtttttca agcccttgaa aatcagcata gcggaaatca tcatcgcgcc
     9721 gaacgaagcg ataaagggaa tccaagaatg cagcgctttg taggaatcga cattgttttt
     9781 acggttgtcg aacgcataaa gaccgcggta atactccgat tgcagctctt gcggatcgaa
     9841 ttcgggttcg tcgtaaattt gcgcgtcgtg cgccattttg gtggcgtact cgactttttc
     9901 ggcttcggac aaaccctcga aaaacaggcg gtgccgttct ttataggcct ttttttcctg
     9961 cttgatgccc ttgagcgtgc cttccgccca agcgttgtaa tctaagacgt ttttcttgac
    10021 gcgcgaaaac aggaaatagg acaccgcgcc gcccaacacg ggcgacaata cccaagaaac
    10081 accaataccg cccagcttgc cccaacgtat caaatcgccc gatgcggcat cgttcattac
    10141 cgccatacat accgcgctgc cgacaatgcc gccgataatg gaatgggtgg tagataccgg
    10201 aagccctttt ttcgaggcaa acaacagcca caacgccgcc gccaaaagcg cggacatcat
    10261 aataaacaca aactgtatgg gttcgaaatc aacacccttc aaatcgacga tgcctttgcg
    10321 tatggtattg gttacctcac cgcccgcgat gaccgcgccg ctgacctcaa ataccgccgc
    10381 aatcagcaaa gcctgcggga tggtcagcgt acccgcaccg acgctggtgc cgaaagaatt
    10441 ggcaacatcg ttgccgccga tgttgaacgc cataaacacg ccgaacatcg tggcaataat
    10501 gaataaggcg gcgtaattgt aatgggtata acccaagccc caataaataa aatagccgac
    10561 tgtacctacc agcatggcgg caaagacggc gttgatggtt ttaacatttg cgctcatttg
    10621 gatttgagcc atggcaatct tcctttaatg tggtggaaat catgagtgtt gccgacactg
    10681 tactgctgcc tccggtcgaa ctgccgtttc gggatatggc agacgcatcc ttccttctga
    10741 accgcatcca aataccccca aacctgaccg aatgcaaaac tgctgccgga aacggtttgt
    10801 atgacacttc tactactgga tgttgcgggc gcattataat atttttccat ccgtcttgaa
    10861 acatttattt acactttatt tacactgcgg cggcaaatcg gtatacgagc gtcaatacac
    10921 gttaaaatgg cgttttgcac cagtttggga gtgatgatgg aaacacagct ttacatcggc
    10981 atcatgtcgg gaaccagcat ggacggggcg gatgccgtac tgatgcggat ggacggcggc
    11041 aaatggctgg gcgcggaagg gcacgccttt accccctacc ccggcaggtt acgccgcaaa
    11101 ttgctggatt tgcaggacac aggcgcggac gaactgcacc gcagcaggat gttgtcgcaa
    11161 gaactcagcc gcctgtacgc gcaaaccgcc gccgaactgc tgtgcagtca aaacctcgcg
    11221 ccgtccgaca ttaccgccct cggctgccac gggcaaaccg tcagacacgc gccggaacac
    11281 agttacagcg tacagcttgc cgatttgccg ctgctggcgg aacggactca gatttttacc
    11341 gtcggcgact tccgcagccg cgaccttgcg gccggcggac aaggcgcgcc gctcgtcccc
    11401 gcctttcacg aagccctgtt ccgcgacgac agggaaacac gcgcggtact gaacatcggc
    11461 gggattgcca acatcagcgt actccccccc gacgcacccg ccttcggctt cgacacagga
    11521 ccgggcaata tgctgatgga cgcgtggatg caggcacact ggcagcttcc ttacgacaaa
    11581 aacggtgcaa aggcggcaca aggcaacata ttgccgcaac tgctcgacag gctgctcgcc
    11641 cacccgtatt tcgcacaacc ccaccctaaa agcacggggc gcgaactgtt tgccctaaat
    11701 tggctcgaaa cctaccttga cggcggcgaa aaccgatacg acgtattgcg gacgctttcc
    11761 cgattcaccg cgcaaaccgt tttcgacgcc gtctcacacg cagcggcaga tgcccgtcaa
    11821 atgtacattt gcggcggcgg catccgcaat cctgttttaa tggcggattt ggcagaatgt
    11881 ttcggcacac gcgtttccct gcacagcacc gccgaactga acctcgatcc gcaatgggta
    11941 gaagccgccg cgttcgcatg gatggcggcg tgttgggtca accgcattcc cggtagtccg
    12001 cacaaagcaa ccggcgcatc caaaccgtgt attctgggcg cgggatatta ttattgagca
    12061 caaatgccgt ctgaaagacc gttccgccgt tcagacggca ttttcgcccc ttttgtttac
    12121 aaacccttaa aattccttta cactcaaaat ccgttcaaca tcaaacaaac cccgctatga
    12181 aaaccctgct cctcctcatc cccctcgtcc tcacagcctg cggcacactg accggcatac
    12241 ccgcccacgg cggcggcaaa cgctttgccg tcgaacaaga actcgtcgcc gcatcgtccc
    12301 gcgccgccgt caaagaaatg gacttgtccg ccctgaaagg acgcaaagcc gccctttacg
    12361 tctccgttat gggcgaccaa ggttcgggca acataagcgg cggacgctac tctatcgacg
    12421 cactgatacg cggcggctac cacaacaacc ccgaaagtgc cacccaatac agctaccccg
    12481 cctacgacac taccgccacc accaaatccg acgcgctctc cagcgtaacc acttccacat
    12541 cgcttttgaa cgcccccgcc gccgccctga cgaaaaacag cggacgcaaa ggcgaacgct
    12601 ccgccggact gtccgtcaac ggcacgggcg actaccgcaa cgaaaccctg ctcgccaacc
    12661 cccgcgacgt ttccttcctg accaacctca tccaaaccgt cttctacctg cgcggcatcg
    12721 aagtcgtacc gcccgaatac gccgacaccg acgtattcgt aaccgtcgac gtattcggca
    12781 ccgtccgcag ccgcaccgaa ctgcacctct acaacgccga aacccttaaa gcccaaacca
    12841 agctcgaata tttcgccgtt gaccgcgaca gccggaaact gctgattgcc cctaaaaccg
    12901 ccgcctacga atcccaatac caagaacaat acgccctctg gatgggacct tacagcgtcg
    12961 gcaaaaccgt caaagcctca gaccgcctga tggtcgattt ctccgacatc accccctacg
    13021 gcgacacaac cgcccaaaac cgtcccgact tcaaacaaaa caacggtaaa aaacccgatg
    13081 tcggcaacga agtcatccgc cgccgcaaag gaggataaac cgtgaaaccg ctgcgaagac
    13141 tcatcaagct ccttgccgcc tgtgccgtag cggcggccgc actcatacag cccgccctcg
    13201 cggcggactt ggcgcaagac ccgttcatta ccgataacgc ccaacggcag cactacgaac
    13261 ccggaggcaa ataccacctc ttcggcgacc cgcgcggcag cgtctccgac cgcaccggtc
    13321 aaatcaacgt catccaagac tatacccacc ggatgggcaa cctgctcatc cagcaggcaa
    13381 acatcaacgg cacaatcggc taccacaccc gcttttccgg acacggatac gaagaacacg
    13441 cccccttcga caaccacgcc gccgacagcg cgagcgaaga aaaaggcaac gttgacgaag
    13501 actttaccgt ataccggctc aactgggaag gacacgaaca tcatcccgcc gatgcctacg
    13561 acggcccgaa gggcggcaat taccccaaac ctacgggtgc acgcgacgaa tacacctatc
    13621 acgtcaacgg cacagcacgc agcatcaaac tcaatccgac cgacacccac agcatccggc
    13681 aacgcatatc cgacaattac agcaacctcg gcagcaattt ctccgaccgc gccgatgaag
    13741 ccaacagaaa aatgttcgag cacaatgcca agctcgaccg ctggggcaac agcatggagt
    13801 ttatcaacgg cgtcgccgcc ggcgcgctca acccctttat cagcgcgggc gaagccttgg
    13861 gcataggcga catactgtac ggaacgcgct atgccataga caaagccgca atgcgcaaca
    13921 tcgccccctt gcccgccgag ggcaaattcg ccgtcatcgg cggcttgggc agcgtggcgg
    13981 gctttgaaaa aaatacgcgc gaagccgttg accggtggat acaggaaaac cccaatgccg
    14041 ccgaaaccgt cgaagccctg gtcaacgtcc tgccgtttgc caaagtcaaa aacctgacaa
    14101 aggcggcaaa accggggaag gctgcggtta gcggggattt ttctgctgca tacaatacaa
    14161 gaacaactag aaaagttact acagaaacag aggggttaaa tagaatcaga cagaaccaga
    14221 aaaatagtaa tatacatgag aaaaattatg gaagagataa tcctaatcat attaatgttt
    14281 tatctggaaa ttctatacaa catatactgt atggagatga agcaggaggt gggcatcttt
    14341 ttcctggcaa acctggtaag acaacattcc cccaacattg gtcagccagt aaaataactc
    14401 atgaaattag tgatatcgtt acatccccaa aaacgcaatg gtatgcacag actggaacag
    14461 gcggcaaata tattgctaaa ggaagaccag ccaggtgggt atcatatgaa acgagagatg
    14521 gaattcgtat cagaacagtt tatgaacctg caacaggaaa agtggtaact gcattccccg
    14581 atagaacctc taatcccaaa tataaccctg taaaataatg agtatttgaa agatgaaaac
    14641 attagacgaa cgaataaaaa atttaggaaa atctcttgaa gatagaattg acgctaatct
    14701 aattgatgcc gcactagagt acattacttt ttcagaacgt ttgctagctt ttgaaacgct
    14761 atgtgattat atagaagatt tcaatatcca gcttacggaa aaagaatccc aagaaatctc
    14821 ttttattaat aaggaatttg gaattgaaag tacatcagat taataaatat taggtcggtt
    14881 taaaaaccca acaatcaaaa agtcgtctga aactgtgttt aatttacaga cgacctttcc
    14941 ttcatttgaa gcaggatatt gagaactgag ttcttcaaaa atcctacacc tgctccttcc
    15001 acggcagcac cttggtcaaa acggcagacg gctacaaagc cattgcccat attcaagccg
    15061 gcgaccgcgt cttcgccaaa gatgaaacca gtggaaaaac tggatacaaa cctgttaccg
    15121 cccgatacgg caatccgtat cgggaaaccg tttacattaa agtttcagac ggcatcgcta
    15181 acagccaaac cctgatttcc aaccgcatcc acccgtttta cagtcaagga aaatggatac
    15241 aggcaggtcg tctgaaaaaa ggcgacaccc tgctttccga aagcggtgca aaacagacgg
    15301 ttcaaaacat tactctcaaa cagcagccgc tcaaagccta caatctgacc gttgccgact
    15361 ggcatactta cttcgtcaag ggcaatcagg cggaaacgga aggggtttgg gttcataatt
    15421 cttgtcctcc caaaagagct cccgaatacc atgcaggaac tgtatctgaa tctaattttt
    15481 taaatgcagc tgagaaatgg ctaggggaaa actataagtc atatccaaat ggtcgatatg
    15541 tatcacaaga tggaatgcgg caggtacgat atggttatca tgaaacaaat tcatctacgc
    15601 atcatggtca ttttgaatca tatgacaaac ctaatggacg tgtaatagaa aatagtgcgg
    15661 taacaattat tcgggattga agaatgatga gtgtaaaaga attattcaaa gtcatcttag
    15721 atgaaaataa agattttcct attagaacga ttcatcgtac cccgatgggg atacttccca
    15781 aacctgttgc tttaagtatt gttcaatatg aaaatgaccc gggattttat ttgttttatt
    15841 tggatgagac cggtcaggaa caaacggata cttatcatga cacattagat tcggcatttg
    15901 aacaggctga atttgaattt ggaatcagca aagaggaatg gatgcaaagt ccttagacgg
    15961 caaaatcaag atgccgcttt atttttcaga cggcatttcc gtcaataaaa aagtcggtat
    16021 tggagcccaa aacagcctac gtcttgccgg gatagggatg aaatctttaa gaaaaaatga
    16081 ttacagtcga acatttagag cattatttgg gtgaaatcga aagcggtata aaatgcttgg
    16141 atcgacgtta tcatctcagc gtttccgtct tcccatcaca accatataaa ggggtaacta
    16201 ctttttctac tttaggcttg aatcgatatg acttgaacta taagagtagg tttgaattaa
    16261 tttttacctg ttctgaagaa tggaataaag aaaatattgc agcttttcta tcaggagtgg
    16321 ctgaatattt gattgacaat agacaaccta ttttaagagg tgaaattatt caattaccca
    16381 gggtaattat cgaaggtagc aaaatggatg cactgtatgt ttccgcccca ttttatttcg
    16441 acgatgattt tcaggtctgt tatggcgaac actacaatat cgttttccct ttgcttgtcc
    16501 ccttgtataa acaggaagcc gaatgggtgg aaaaaaaggg ttggaatgct tttgagcagt
    16561 tcttgctgga taatgaagtt gacaaccttt cggatatgaa tcggaaaccg tttgcttggt
    16621 aaggattttc ttaaatttaa tcaaagtgcc gtctgaaacc gtgtttaatt ttcaggcgac
    16681 cttcccctta ttttaaacag gatattggga actaagttct tcaaaaatcc tacacctgct
    16741 ccttccacgg cagcaccttg gtcagaacgg cagacggcta caaagccatt gcccatattc
    16801 aagccggaga ccgcgtcctt tccaaggacg aggcaagcgg agaaacggga tacaaacccg
    16861 ttaccacccg atacggtaat ccgtaccagg aaaccgttta cattgaagtt tcagacggca
    16921 tcggcaacag ccaaaccctg atttccaacc gcatccatcc gttttacagt caaggaaaat
    16981 ggatacaggc aggtcgtctg aaaaaaggcg acaccctgct ttccgaaagc ggcgcaaaac
    17041 agacggttca aaacattacc ctcaaacagc agccgctcaa agcctacaac ctgaccgttg
    17101 ccgactggca tacttacttc gtcaagggag ataaggcgga aacggaaggg gtgtgggttc
    17161 ataatgagtg tccgaccaaa ccaaaaccaa ctaatcatgc ccaacaaaga aaagaagaag
    17221 ctaaaaacga ttctcatcga agtgtgggag attccaatcg tgtcgttcgc gaaggaaagc
    17281 aatatttaga tgccgacaca ggaaaccatg tttatgtaaa aggagaaaaa gtggttattc
    17341 taactcctga tggaagacag gtaactcaat ttaagaactc gaaagccaat acgtcaaaaa
    17401 gggtaaaaaa tggaaaatgg acaccgaaat aatcgatctc ccttagaaaa aagaattttt
    17461 tatctggagc attcgggaca gtatctgatg atttgcgccc tatcagatta ctccaaaaat
    17521 aaacatactg tcgttatggc aaattttctc tatccaaatg aaaaaatgga ttggagaaat
    17581 ttagatgatt tgttcaatga gttggtttta gaggaattac agtcttcatt tatggattgg
    17641 tatccaacta ttgaagaggc aattagccat catttagaag atttttcgta acaagcataa
    17701 atcaaaaagt cgtctgaaac tgctttta
//
LOCUS       DAWKQB010000030        17123 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.030, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000030 DAWKQB010000000
VERSION     DAWKQB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.030"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..1220)
                     /locus_tag="WJL14_000743"
     CDS             complement(51..1220)
                     /locus_tag="WJL14_000743"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ7655844.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPSIAYEELKNAVIEQIIKPVLPS
                     ELLTAETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLIALV
                     REHFDLRPKGIVQMLDLLRPIYGKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1403..1513)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1566..2462
                     /locus_tag="WJL14_000744"
     CDS             1566..2462
                     /locus_tag="WJL14_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ7655845.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGLNPDTQTVKAVFAETAKGGLELAPAFFKKPEDIETMF
                     KAVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     VMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPDGQGFALHISPFQGKLTGDKTHDAAVFNRN
                     TEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WJL14_000745"
     CDS             complement(2506..3570)
                     /gene="tsaD"
                     /locus_tag="WJL14_000745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ7655846.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALAFAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WJL14_000746"
     CDS             complement(3687..4874)
                     /gene="ccsB"
                     /locus_tag="WJL14_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ7655847.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4867..6819)
                     /locus_tag="WJL14_000747"
     CDS             complement(4867..6819)
                     /locus_tag="WJL14_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ7655848.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKGAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7096..7719)
                     /locus_tag="WJL14_000748"
     CDS             complement(7096..7719)
                     /locus_tag="WJL14_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ7655849.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7925..8554
                     /locus_tag="WJL14_000749"
     CDS             7925..8554
                     /locus_tag="WJL14_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ7655850.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8675..11071)
                     /locus_tag="WJL14_000750"
     CDS             complement(8675..11071)
                     /locus_tag="WJL14_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ7655851.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11223..12338
                     /locus_tag="WJL14_000751"
     CDS             11223..12338
                     /locus_tag="WJL14_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ7655852.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKLLYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFDQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12341..12940
                     /locus_tag="WJL14_000752"
     CDS             12341..12940
                     /locus_tag="WJL14_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ7655853.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12941..13588
                     /gene="pilO"
                     /locus_tag="WJL14_000753"
     CDS             12941..13588
                     /gene="pilO"
                     /locus_tag="WJL14_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ7655854.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLNAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13606..14151
                     /locus_tag="WJL14_000754"
     CDS             13606..14151
                     /locus_tag="WJL14_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ7655855.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14170..16407
                     /locus_tag="WJL14_000755"
     CDS             14170..16407
                     /locus_tag="WJL14_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ7655856.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSEPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAAPAKQTNIDFRKDGKNAGIIEL
                     AALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKTPVQKVTLKRLNNDTQL
                     IITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKTFTGRKISLDFQDVEIR
                     TILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQARNLDMRQQGNIVNIA
                     PRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRSILRLDNADTTGNRNTL
                     VSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQVMIEARIVEAADGFSRD
                     LGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINLPITAAANSISLVRAIS
                     SGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIPFTVTSIANGGSSTNTE
                     LKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTILCISTKNLNTQAMVENG
                     GTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDRRELLIFITPRIMGTAG
                     NSLRY"
BASE COUNT         4602 a         4860 c         3906 g         3755 t
ORIGIN      
        1 aaaatgccgt ctgaactgtt cagacggcat ttttgatttt caaaccggaa ttacagcccc
       61 gctgccgccc tcaatgcagc agctttgtcg gtgcgctccc aagtgaactc aggttcttcg
      121 cggccgaaat gtccgtaagc ggcggatttt ccgtaaatcg ggcgcaagag gtcgagcatt
      181 tggacgatgc ctttggggcg caggtcaaaa tgttcgcgaa ccagcgcaat cagtttttcc
      241 tcgctgattt tgcctgtgcc gaaagtgtcg atggaaatcg aagtcggttc ggcaacgccg
      301 atggcgtagg aaacttgaat ttggcattgg gttgccaagc ctgcggcgac gatgtttttc
      361 gccacataac ggcaggcgta ggcggcggaa cgatccactt tggacgggtc tttgccggag
      421 aacgcgccgc cgccgtgcgg agccgcgccg ccgtaggtat cgacgatgat tttgcggccg
      481 gtcaaaccgc agtcgccttg cgggccgccg ataacgaaac ggccggtcgg gttgatcagg
      541 tatttggttt cggcggtcag cagttcagac ggcaggacgg gcttgataat ctgctcgatg
      601 acggcgttct tcagctcttc gtaagcgatg gacggatcgt gttgcgtaga gaggacgacg
      661 gtatcaatgc gttttacctt accggtttcg ctgtcgtaaa ccacggtcag ttgggctttg
      721 gcatcaggac gcagccacgg caggcggccg tctttgcgca attcgctttg gcgctgcatc
      781 aggcggtggc tgtaatagat ggcaaacggc atcagggtcg gggtttcgtc gcaggcatag
      841 ccgaacatca agccttggtc gcccgcgcct tggttcaggt cgatgccttc gccttcgttc
      901 acgccttggg cgatgtcggg agattgctgg tcgtagtaca cgccgactgc gcagccgttg
      961 gcatcaaagc ccagctcgga ggagttgtag ccgatgcgtt tgatggtttc gcgtgcgact
     1021 ttgatgtagt ccacttgggc ggtggtggtg atttcgcctg ccaatacgca caagcctgtg
     1081 ttgaccaaag tttctgcggc gacacgcgct ttggggtctt gcgccaagat ggcatccaaa
     1141 atcgcatcgg acacttggtc ggcaactttg tccggatggc cttcggatac cgattcggaa
     1201 gtaaacagat attcgctcat tgagattttc cttgaaaaac aaaccatctt cttcagacgg
     1261 catgttgtat gaacataatg tcgacagcgg gaaatatagc aaaatttccc tattcatacc
     1321 attcagttga gaaatattcc catttgaatg gcactttgga atctctgccc gtacgtttct
     1381 tacaggcaaa aaaattcccg catcaagcgg gtttggattg ctctggtgag ccacatcggc
     1441 ttttcaaccg tccaccttac ttttcctttt gaaaagcagg ttggcatgga attcccaact
     1501 cttaatgcag cgcggatggt agcagaaaag cctttccgcc gcaatacttc cctttttcag
     1561 acggcatgtt tcgtttacaa ttcgggctgt ttcccccttt gcgaaccgcc atgcacatcc
     1621 tgttgaccgc cctgctcaaa tgcctctccc tgctgccact ttcctgtctg cacacgctgg
     1681 gaaaccggct cggacatctg gcgttttacc ttttaaagga agaccgcgcg cgcatcgtcg
     1741 ccaatatgcg gcaggcgggt ttgaaccccg acacgcaaac ggtcaaagcc gtttttgcgg
     1801 aaacggcaaa aggcggtttg gaactcgccc ccgcgttttt caaaaaaccg gaagacatag
     1861 aaacaatgtt caaagcggta cacggctggg aacacgtgca gcaggctttg gacaagggcg
     1921 aagggctgct gttcatcacg ccgcacatcg gcagctacga tttgggcgga cgctacatca
     1981 gccagcagct tccgttcccg ctgaccgcca tgtacaaacc gccgaaaatc aaagcaatag
     2041 acaaagtgat gcaggcaggc agggttcgcg gcaaaggaaa aaccgcgcct accagcatac
     2101 aaggggtcaa acaaatcatc aaagccctgc gttcgggcga agcaaccatc gtcctgcccg
     2161 accacgtccc ctcccctcaa gaaggtgggg aaggcgtatg ggtggatttc ttcggcaaac
     2221 ctgcctatac catgacgctg gcggcaaaat tggcacacgt caaaggcgtg aaaaccctgt
     2281 ttttctgctg cgaacgcctg cctgacgggc agggttttgc cttacacatc agcccctttc
     2341 aaggaaaatt gaccggcgac aaaacccatg atgccgccgt attcaaccgc aataccgaat
     2401 attggatacg ccgctttcct acccagtatc tgtttatgta caaccgctac aaaatgccgt
     2461 aacgaaaata aaaatgccgt ctgaacaatt tcagacggca ttttgtcatt tgacgatttc
     2521 cgacagtggc cagcgcggac ggacattgaa cgcaccgacc tccctgccct tgcccaggtg
     2581 catcgcaccg gcaaaggcaa tcatggcacc gttgtccgtg cagtatgccg tcggcgggaa
     2641 aaacacgctg actttttcgg acggatgttt cggcttgcct gttggggtcg ggatttgcac
     2701 cgtcatgctg ccgaaagttt cacggagttt gcggtttgca ccgaccccgc cggcgaccac
     2761 tacggttctg aaccctgtct gcaacagggc ttttttcact ttcgccgcca acacatcgac
     2821 taccgcatct tgaaacgcac ggcagatgtc gttgcgtgtc tgctcaggaa tgtcatccgc
     2881 cccgttttcc gcgcgcactt tctcgacggc agtcaaaaca gcggttttca aacctgaaaa
     2941 actcatctgc aaatcgtcgg aatgaatcat cgggcgcgga aaaacaaacg cttcgggcct
     3001 gcccgattcc gcaagttccg acagtttcgc accgcccgga tacggcaagc ccagcagttt
     3061 cgccgttttg tcgaatgcct cgcccgccgc atcatcgacg ctctcgccca aaagcgcgta
     3121 gtcgcctatg cccctgaccg ccataatctg cgtatgcccg cccgaaacca acagcgcgac
     3181 aaaaggaaag tcgggttttt cctccgccaa cagcggcgac agcagatgcc cttccaaatg
     3241 atggacggga ataacaggct tatccagcgc aaaagccaag gcattggcgt aactcgaacc
     3301 cgccagcaac gcgccgccca gaccgggtcc ctgcgtaaag gcaaccgcgt caatgtcgcc
     3361 atacgatgca cccgcctgcg ccaaacagcc ttccgtcaac ggaacaaggc ggcggatatg
     3421 gtcgcggctt gccagctccg gcacaacccc gccgtattcg gcgtgcattg ccatctgcgt
     3481 gtgcagacag tgcgcccgca atccacgttc cgtatcgtaa agcgcaacac cggtttcgtc
     3541 gcaagaagac tcgattccta ataccaacat ggtctgatgc cgttaaaaac tgaaaaacgt
     3601 attttagcgg atttcggcac gactgccgta tcccaaaaac ggaacatgcc gtctgaagac
     3661 cgttcagacg gcatcgtcgc accgtatcaa agcgttccgt aagaatgcag cccgctcaaa
     3721 aacatattca cgccgataaa ggcaaatgcg gttacgaaca aaccgataat cgcccaccac
     3781 gccagcactt tgccgcgcca accggcaacc agccgcaagt gcagccaaac ggcgtaattg
     3841 agccagacga tgaacgccca cgtctctttc ggatcccaac tccaatagcg tccccaagca
     3901 tctgccgccc acagcgcacc caaaatggtg gcaatggtaa agaacagaaa gccgacggca
     3961 atcgccttat acatcacctc gtcgatcaat gccgacggcg gcagccacag ttttccgcct
     4021 tttccttccg cacgcaggga aaccagttcg gcaataccga gcatcgcgga aatgcaaaac
     4081 gcgccgtaac cgataaagtt tgccggaacg tggattttca tccaccagga ctggagcgcg
     4141 ggaatcagcg gctggatggt atgcgcctcg cgggacacgc tgtaccacaa gacaaatcca
     4201 accacgaccg ccataaagcc gaacacgaag ccgcccaatt tctgtatggc gaacttacct
     4261 tcataataaa gatacatcag cgcggtaatc accaaaaaca ggatgaacac ttcatacagg
     4321 ttggaaaccg gaatatgccc cgcatcggga cggagcagat agctttcgtg ccaacgtacc
     4381 agcagaccgg taaagcctgc tacggcagac acccatgcaa acacggttcc catacccaac
     4441 agcgtgttgg tcggcacatt ttttacgctt gccaaaaccg cgcccgaaat ataggcgaac
     4501 agggcgaaga agacaaaggc gcactgccac atgatcgccg actggctgct gaggaaatac
     4561 cgcaacagga aaatctctgc cgatttgatg ttgccgccat acaaaccgac ggcggcatag
     4621 gcaagcaata cgcttaaagg aacaaaccag cgcatcggtt tgaaaaacca acccaaaaac
     4681 acggcaatac cggcacttgc ccacaacatg accgtttcgt aaatgtccat atgcataccg
     4741 gaacgggtct gtacgaaaac cgtagccgca aaaaccagca cggcaaatac ccaatcccaa
     4801 agattcagat tgctgatcaa agacttctga atcagcagct cgtgttccgg aagggtttta
     4861 tagtgttcag tcatgattca agtccttgcc gagccgttgc agactctcga cgtgttttgg
     4921 aaattccttc tgcaaatccc gttcgctgcg ggccgaagac atggcaaaac ggattttgcc
     4981 gtctgaaaac aatacccacg cccgtttttc gcgcacataa aacatcaata ccgtacccaa
     5041 taccaacagc accgagccga gatagaccaa aagcgcaccc ggggaacggg tcatctgcaa
     5101 acccgacgaa cgcacctcgg aaaacccatc aagttgcagc agcataggcg cgggatattc
     5161 ggtcaaaccc gtgtacgcat ccatactgtg cagcaggaaa cgattccgcg cttcatcctg
     5221 ctgccattcg ggcaagccgt accggcgtat ggtttcatcc aaagcagcgt tcatcacgcc
     5281 gtaaagcatt tcgtagaaat agccctgcat cttatcctgc tgctctttcg ggatattgga
     5341 cgtaataaat tcgtccaatc ccaaatagcc tttttgtgca aagatgttca gcgtgttttc
     5401 cgcagccagc atgaattgtt cgcggatttc ggcaggtgcg cctttggttg cgtcggcaac
     5461 cagacgtttg cgcccttccc catctttcaa aaactcacgc aatgccataa aggtgtccgc
     5521 tttcaactgc ttgtccaagg ggatacgcag ccagcggtat tgctgctgca agccgctgcg
     5581 cgtgccggta atccaaaaat aatcctgttc ctgcaaaacc ggcagcatat agtttttata
     5641 ttcgaccgcc tgccctgccg catcacggat acggtaaaca atggaagggc cgatattggt
     5701 gtatttttta ccttcctgag taacggcgcg gacatcgttc agcgtggatt tcaggctttt
     5761 ttcccgttcc gcgccctcgc tcatgtcctc cacattcata gaagtaaact gatcgaactc
     5821 aagacgatat ttgtgtttgc caatttccaa cggaaactgg tgtatggatg ttgccttcaa
     5881 cacgacaggc tcgcgcgaag catcacccaa attccacgcc ttgaatgtca aatccgaacc
     5941 gccgtcggca aaactcgcct gataaatcgt gatgccgtgc aaggtcaaag gatggttcac
     6001 gcggatggtg cgctcgagtt tctcaccggt tgccttatcc gttacttcaa tatcactggc
     6061 aaaatcgcgc ggcatacccg tattgtaaaa atcgatatgg aattttttca gtttgacttc
     6121 aaaaggcaag tcctgaacca atatcccgtt gtcggcattc aggaaaacca catccgcact
     6181 ctgcccctcg gaaatattga cgttgcccct aaatgagaga ttggacgcac ccaaaatact
     6241 ttcgggcttg aaatccttgg cataaaccgc ctgattgtcc ggaacaatcc gaccggtcag
     6301 catacccagt ttcaacagca ggttactgtc tatcaacccg cccaggcaaa tgacaatcaa
     6361 agcaacatgg gcaaagatat agccccattt gttcattgtg ccttttttgg cggcaatcag
     6421 aaccgacccg tcttcacggt taatggtttt tccctgaaaa ccttgtactt ccagataacg
     6481 tttggcaacc tcgggcgcaa tttttacatc caacagcgaa gaatggcgca tcgccgccag
     6541 agatttttct ttaacctttt cccgaaaaga cttcatttcg cgccagaacg gcggcacatt
     6601 gcgaatcagg cacaaactgg tagaaaccac caaaaacatc atgataacga caaaccatgc
     6661 cgaagcatag acgtcataca gtcccagaaa accaaaaatc tgcgcccaaa acgatccgaa
     6721 tttgaccaaa taatccgtct gcggctggtt ttgctgcaac accgtaccga taaccgatgc
     6781 aatacccagc agactgagca aagcgaccgc aaagcgcatg gagctgaaaa aagcgaacca
     6841 cggacgggaa agaagtgggg gagatctacg ggatttactc attgtgtgtt tattccgcca
     6901 tcaggaatat gggaaagcag aattgggcaa acagaaaaca acgtcccgat tctactgtct
     6961 tgatgctttt tatttcaaga caatgaagac agcctgcatc gattccaacg gttgcgattg
     7021 aaaaaactta tcgcagaatt gcctgaagcc gtctgaaaac ttttcagacg gcctctaaaa
     7081 cagactattg cggaattaac gcaaaccttg gataaagttg gcgaccgctt tcaaatcttc
     7141 ttcagacata cggtttgcaa tatcttccat gatggtattt ttacgctgac cggacttgta
     7201 ggcattcatc tgttcaacaa tatatgcctg atgctgaccg cccaaacgcg gataagcctg
     7261 aatttcgctt ccgcctcccg gcatacccgc accgctcgga ccgtggcagg acatacacgc
     7321 cggcactttt ttatcgctca aaccgccgcg atagattttc gcacccaatt cgggattttc
     7381 cttaggattg gcttcaccgg atttgggctg ctgtttggca tagaatgcgg atacgttcaa
     7441 aatatcctga tcgctcaaat tcattaccac cggtttcatc acagctgccg aaccgtgggt
     7501 gcgtttaccg tcgcggatgc cgatggtttg atgatagatg taagcagtat gctgtgccgc
     7561 caaacgcgga tacatcgcaa tgccgctgtt accgtctgct gcatggcaag ccgcacaaac
     7621 cgttgcggca acctgtttgc ctttttccac gtctgctttg ggagatgcgg aaaccgcacc
     7681 ggcagccaaa acaaaggcca ataaagtcaa tcgtttcatg gagtgctcct gattacagca
     7741 ttggataacg caacaatgct ctttttatat tcaaatacgg gatttttgac ccgattaaaa
     7801 ccgatgattc tgcaaacgtg ttattctata ctaaatttac attaaattac cactgtgttt
     7861 cacataaaac caaccgcata tttttgctgt cggacaaacg gcggcggaaa acaaggatat
     7921 gcccatgaac ctttttcaaa acgccaaatt cttcacgacg gtaaaccatc tcaaagacct
     7981 gccggacaca ccgctcgaaa ttgcctttgt cggcaggagc aatgccggaa aatccagtgc
     8041 catcaatacc ctgaccaacc atgtccgtct tgcctacgtt tcaaaaacac ccggacggac
     8101 gcagcatatc aacttcttcg agctgcagaa cggcaatttt atggtcgatt tgcccggcta
     8161 cggttatgcc caagtccccg aagcagtacg cgcacattgg gtcaatctgc tcggcgacta
     8221 tctgcaacag cgcaaacagc ttatcgggct ggttttgatt atggatgccc gccatccttt
     8281 aaaagaactc gacatccgta tgctggactt tttccacacg accggcagac cggttcacat
     8341 cctgctgtca aaagccgaca aattatccaa aaacgaacag ataaaaaccc tgtcccaagt
     8401 caaaaaactg ctcaaacctt attccgacag gcaaaacatc agcgtacagc tgttttccag
     8461 cctgaaaaaa caaggtattg acgaggccaa ccgaactgtc ggaagctggt ttgacgcagc
     8521 agatgccgcc gcttcctctc caaaggaaaa ctgaccccaa ttatacggaa accgtattcc
     8581 ccccacttga ccgaccgcaa acatttaaaa aattgccact gccaaatcta aaatgccgtc
     8641 tgaaagactt ttcagacggc attttgcgga gtctttaaaa cagagaatcc aactgctgct
     8701 gtttggaacc agtattactc ggaagcaccg gcgtttcctg catatcttgg cggacttcgt
     8761 catccgccgc ctgccgtccg ccttctgccg cgcccccgtc atcttctttt gcccgtcggg
     8821 aaggttgcgg cgcaataccg ctgttgtcca gcgtcaagcc cggatcggtt accatacgtt
     8881 ccttcatata gtattcgcca ttgctgctga ccacaccttc aggcattttc atccccttgc
     8941 cctgctttcc tttcaacgca aaacgcatat agtccaccca aaccggcacc gcaatcgtac
     9001 cgccgtagcc gacacgcccc atactcttag gtttgtcgaa gccgatatat acggcagtaa
     9061 ccacatcagg gttaaaaccg acaaaccacg catccttatt gtcattggtc gtacccgttt
     9121 taccggcaat atccgttctt cccaacgcag ctgcccccct tgccgtacca acacggacca
     9181 catcctgcat aatcttatac ataatatagg cattgcgcgg atcgattgcc tgaggcgcat
     9241 tttgcccggc caccaaaggt tgcatttggg cgcgcaacct gccgtctctg tcataaatct
     9301 tatcgattac gtgcgaagaa accctatatc cgccgttcgc aaatacgcta tatgcctccg
     9361 ccactttcaa cggcgttgtc tcgcccgtac ctaaagccat agacaggctt gccggcagct
     9421 cggacgacct gaagccgaaa cgccggatat actgttgcgc gtaaccgaca ccgatagaca
     9481 tcaaaatacg gatggaaacc atattcttgg aagccgtcag agcctgtctc aaagtaatgt
     9541 agccggaata tctgccgtct gaatttttag gtgtccaaac cgaaccgttc ggccctttcc
     9601 ccggcaggga aatcggcgca tcgttaacca ctgtggacgc ggtcatcccc ttagataatg
     9661 ccgccgaata gacaaacggc ttaaaggtcg aacccggctg ccgcattgcc tgaacggcac
     9721 gattgaatgt tttgctgtga aaatcataac cgccgaccag cgcgcgcaca gctccggttt
     9781 ttgcatccag cgaaaccaaa gccccctgca gcaacggctc ttgaaccacc gcccaacgcc
     9841 cgccgttgtt tttgacacgg atgaccgcgc ccctgcggat acggtcctcc cccatttttt
     9901 cattattgac cgcgcgggcc gcaaaaccca aggcgcgcct gtcaagcgta acccgcctgc
     9961 cgccgggcag ctgtatgacg acatttttct ttttagtcac atccaacaca acggcgggaa
    10021 ccattttatc gacggtatag agtcccgaca gatactggct gacagtctcc tcgacatctt
    10081 cactcttact caaatcgata tagttttccg caccgcggta gctgctgccg cgatcgaaat
    10141 tccgtagagc cttgcgcaat gcctcggttg ccaccttctg atgatcggcg cggaccgtgg
    10201 tataaacctt aaaaccctgc gtataggcat cttcaccgta tttctcatac agttcctgac
    10261 gcaccatttc cgccacatat aaagcactct gatcgatttt ccgaacaaac cgctcgtaat
    10321 gcagttcctc attcaacgcc tgatcgcgct gttgcacggt aatcatcttc tcctcgagca
    10381 tattgttcaa aatatacttc tggcgcaact tggcacgttc tggattaaca atcggattat
    10441 aggcagacgg agccttgggc agtcccgcaa gcatggcggc ttccgccaaa gtcaaatctc
    10501 ggacattctt attgaaatag atttgcgcgg cagatgcaaa accataggcg cgctgaccga
    10561 ggtaaatctg attgaaatac aactcgagga ttttgtcttt gcttaaagac tgctcgattt
    10621 tataggcaag caacacctca ttgaatttgc gtgtgaacgt tttttcactg ctcaaataaa
    10681 aatttttcgc cacctgctgc gtaatcgtac tcgcacccga ctgcacgctg ccggacacga
    10741 cattgccgac ggcagcgcgg gcaacacccc aaacatccac cccccaatgc cggtaaaagc
    10801 gtttatcctc ggcggcgata accgcattcc gcaacacctc tgggaaatcg ccgatttttg
    10861 taaattcgcg ccgctgctcc ccatacatac cgatgacttc cccatccgcc gaataaatag
    10921 tcaacggcat tttaggctgg taatgctgca aagaatccaa agacggcagt ttcggatacg
    10981 ttaccaaaat agcaatggca accaaaccca ctccaaatac acaaaaccca aaaaccaaac
    11041 caaaacaagt cgttaaaatc tttttaatca tagctgaata ataatttacc attattggta
    11101 ttaaataaag taaaatagca accgatttct acaaagcacg gtttcaatgt gcaaagaaca
    11161 aggaatccat tacggatacc gaaacggtta ctcactgtac aaataaagca ggaactttca
    11221 tcatgcgctt gtttaaaagc ttgaaaaacc ctaaaaaaac agatgccaag ctccctaaaa
    11281 aatcttcggg actcaataac cgcgcggcaa tcggcatcga tatcgaccag cattccatca
    11341 aaatggtcca attgtcagga cgtagtttaa accaaattca attggaaaaa tacgtcattg
    11401 ccaaattacc aaagaatatc attcaaggca ataaagtcca aaattacgat caacttgtta
    11461 catatttgca acaagcctat gccaaactgg gtacttcgtg caaaaacatc atcgcgtccg
    11521 tcccgcaaaa tctggcaacc atcgaacaat tgacctacac agacaaagat gcagaattag
    11581 acctgcaggg gttcgtggag tcctccatct ccgaagtcag ctcgatatcg ctcgaagaag
    11641 ccaattacga ctatcaggtc ttgtcccaat cggccgccgg cgaagctgtg ttggccgtcg
    11701 catcgagaaa ggatgaaatc gaacccctga ttgacgcatt caacgccgcc ggtatgaaat
    11761 tatccgcgct tgatgtggac attttcggac aatacaacgc ctacgcgcta tggataaacc
    11821 atttcgcccc cgagcttgca gccgaaaaag tcgccatttt cggcgtatat gccgcacaga
    11881 cctacgcctt ggtcatccaa gacggaaaac tcctatacaa acaggaaacc tccgtcagcg
    11941 aagaacagct caaccaactc atccagcgca cctatcaggt aacagaagaa aaagcggaag
    12001 aaatcatcaa ctccccgcaa aaaccttccg attaccaaga aagcgtggca aactatttcg
    12061 accagcagat tacccaagaa atacaaaggg tcttgcagtt ttattacacc acgcagaccg
    12121 cagacgatat gaccgacatc aagcatatcc tgctgaccgg ggaagcagcg cgccaggaag
    12181 gcatcgccca aaccgtcgcc tcacaaacca atgcagatgt acaatgcgtc catcccgcgc
    12241 gttattttgc ggacaacctc aaaacagaca aacaacaatt cgaacttgat gcgccgacac
    12301 tgaccagggc gttcggtttg gcggtacggg gattataatt atgaacaatt taatcaaaat
    12361 caacctcctc ccctacaggg aagagatgaa caagcgcaaa cagcagcagt ttaaaacgct
    12421 gatgtacggt gccgtgctga cgggcgttgc cgccgttgcg gcaacctacc tgtttatcga
    12481 caatatgatc aataaccagt cggaaagaaa cacgctgctg gaaacctcca tcgcacactt
    12541 ggataccgag ctgtcggaaa tacaaaagct caaacaggaa aaagatgcct tcctgattaa
    12601 gaaaaacaaa atcgaggagc tccagctcaa acgcctccaa gccgcaaaaa tcctcgacag
    12661 cctgaatgag gccgtccccg gaagcaccta cctgacctcg ctggatgccg ttaccgccga
    12721 ctcttatcgg ctcagcggca ggacatccag cgacaaccgc gttgccgcca tgatgagggc
    12781 gatgcccaat accggcatat tcaagcaacc cgaattgtta agcatcaaga aaaacaattc
    12841 gcatcaagaa tttacccttc aggcaacatt acaacccatc gtaaaggcgg ccgaatccaa
    12901 agagaatccg gcttcgggaa acgcacagga ggcaaactga atggcttcta aatcatctaa
    12961 aaccaacttg gatctcaaca accttcacct gctcaacctt cctgccaggc tttttatcgc
    13021 cctgctggcc gttgccgccg tgctggggct cggttatgcc ggattgttca aaagccagat
    13081 ggaatccctt gaggaatacg aagcaaaaga aaccgaactg aaaaacacct acaaacagaa
    13141 aagtatcgac gcggccagcc tgaacaacct gagggacgaa cttgcctcaa tccgctctgc
    13201 cttcgatatc atgttgaaac agctgccgac agatgcagaa attcccaatc tggttcaaga
    13261 gcttcatcag gcaggttcga gcaacggtct gcgcttggac agcgttatgc cccaacctcc
    13321 cgtagatgac ggccccatca aaagattacc ctattccatt tccattaccg gaaattacga
    13381 acagatcagc caatttaccc gcgatgtcgg cagcctctcc cgaatcatta cccttgagtc
    13441 gctgaaaatc gcccaatctc cggaaaacgg cggcaatcct gacggcaaga gcagcatcct
    13501 gaacctcaac gccattgcca ccacctacca agcaaaatcc gtagaagagc ttgccgcaga
    13561 agcggcacaa aatgccgagc aaaaataact tacgttaggg aaaccatgaa acactatgcc
    13621 ttactcatca gctttctggc tctctccgcg tgttcccaag gttctgagga cctaaacgaa
    13681 tggatggcac aaacgcgacg cgaagccaaa gcagaaatca tacctttcca agcacctacc
    13741 ctgccggttg cgccggtata cagcccgccg cagcttacag gaccgaacgc attcgacttc
    13801 cgccgcatgg aaaccgacaa aaaaggggaa aatgcccccg acaccaagcg tattaaagaa
    13861 acgctggaaa aattcagttt ggaaaatatg cgttatgtcg gcattttgaa gtccggacag
    13921 aaagtctccg gcttcatcga ggctgaaggt tatgtctaca ctgtcggtgt cggcaactat
    13981 ttgggacaaa actacggtag aatcgaaagc attaccgacg acagcatcgt cctgaacgag
    14041 ctaatagaag acagcacggg caactgggtt tcccgtaaag cagaactgct gttgaattct
    14101 tccgacaaaa acaccgaaca agcggcagca cctgccgcag aacaaaatta agaagaggat
    14161 tactccatta tgaataccaa actgacaaaa atcatttccg gtctctttgt cgcaaccgcc
    14221 gcctttcaga cagcatcggc aggaaacatt acagacatca aagtttcctc cctgcccaac
    14281 aaacagaaaa tcgtcaaagt cagctttgac aaagagattg tcaacccgac cggcttcgta
    14341 acctcctcac cggcccgcat cgccttggac tttgaacaaa ccggcatttc catggatcaa
    14401 caggtactcg aatatgccga tcctctgttg agcaaaatca gtgccgcaca aaacagcagc
    14461 cgtgcgcgtc tggttctgaa tctgaacaaa ccgggccaat acaataccga agtacgcggg
    14521 aacaaagttt ggatattcat taacgaatcg gacgataccg tgtccgcccc cgcacgcccc
    14581 gccgtaaaag ccgcgcctgc cgcaccggca aaacaacagg ctgccgcacc gtctaccaag
    14641 tccgcagtat ccgtatccga accctttacc ccggcaaaac aacaggctgc cgcaccgttt
    14701 accgagtccg tagtatccgt atccgcaccg ttcagcccgg caaaacaaca ggcggcggca
    14761 ccagcaaaac aaaccaatat cgatttccgc aaagacggca aaaatgccgg cattatcgaa
    14821 ttggcggcat tgggctttgc cgggcagccc gacatcagcc aacagcacga ccacatcatc
    14881 gttacgctga aaaaccatac cctgccgacc acgctccaac gcagtttgga tgtggcagac
    14941 tttaaaacac cggttcaaaa ggttacgctg aaacgcctca ataacgacac ccagctgatt
    15001 atcacaacag ccggcaactg ggaactcgtc aacaaatccg ccgcgcccgg atactttacc
    15061 ttccaagtcc tgccgaaaaa acaaaacctc gagtcaggcg gcgtgaacaa tgcgcccaaa
    15121 accttcacag gccggaaaat ctcccttgac ttccaagatg tcgaaatccg caccatcctg
    15181 cagattttgg caaaagaatc cgggatgaac attgttgcca gcgactccgt caacggcaaa
    15241 atgaccctct ccctcaaaga cgtaccttgg gatcaggctt tggatttggt tatgcaggca
    15301 cgcaacctcg atatgcgcca acaagggaac atcgtcaaca tcgcgccccg cgacgagctg
    15361 cttgccaaag acaaagcctt cttacaggcg gaaaaagaca ttgccgatct gggcgcgctg
    15421 tattcacaaa acttccaatt gaaatacaaa aatgtggaag aattccgcag catcctgcgt
    15481 ttggacaatg ccgacacaac cggaaaccgc aatacgcttg tcagcggcag gggcagcgtg
    15541 ctgatcgatc ccgccaccaa taccctgatt gttaccgata cccgcagcgt catcgaaaaa
    15601 ttccgcaaac tgattgacga attggacgta cccgcgcaac aagtgatgat tgaggcgcgt
    15661 atcgtcgaag cggcagacgg cttctcgcgc gatttgggcg ttaaattcgg cgcgacaggc
    15721 aagaaaaagc tgaaaaatga tacaagcgca ttcggctggg gggtaaactc cggcttcggc
    15781 ggcgacgata aatggggggc cgaaaccaaa atcaacctgc cgattaccgc tgccgcaaac
    15841 agcatttcgc tggtgcgcgc gatttcctcc ggtgccttga atttggaatt gtccgcatcc
    15901 gaatcgcttt caaaaaccaa aacgcttgcc aatccgcgcg tgctgaccca aaaccgcaaa
    15961 gaggccaaaa tcgaatctgg ttacgaaatt cctttcaccg taacctcaat cgcgaacggc
    16021 ggcagcagca cgaacacgga actcaaaaaa gccgtcttgg ggctgaccgt tacgccgaac
    16081 atcacgcccg acggccaaat cattatgacc gtcaaaatca acaaggactc gcctgcgcaa
    16141 tgtgcctccg gtaatcagac gattctgtgt atttcgacca aaaacctgaa tacgcaggct
    16201 atggttgaaa acggcggcac attgattgtc ggcggtattt atgaagaaga caacggtaat
    16261 acgctgacca aagtccccct gttgggcgac atccccgtta tcggcaacct ctttaaaaca
    16321 cgcgggaaaa aaaccgaccg ccgcgaactg ctgattttca ttaccccgag gattatgggt
    16381 acggccggca acagcctgcg ctattgatgc gtcaaaataa gggcatatgt tttacggcat
    16441 atgccctttc tttatgcttt ttgccgcgac cgaaatgccg tcattcccgc gcaggcggga
    16501 atccaacttg tccggttccg gttgtttttc gtctcgtaac ttttgagccg tcattcccgc
    16561 gaaagcggaa atccagtccg ttcggtttcg gtcgtttccg ataaattcct gctgcttttc
    16621 atttctagat tcccactttc gtgggaatga cggtggagag gtttttgttg tttcggagaa
    16681 gtttctgcaa ccctagaatc tcgttatttc cacaaaaaac agaaacccga aacagcaact
    16741 taaaacctcg tcattcccgc aaaagcggga atccggtccg ttcagtttcg gtcgtttccg
    16801 ataaattcct gctgcttttc atttctagat tcccactttc gtgggaatga cggcggagag
    16861 gtttttgttg tttcggagaa gtttctgcaa ccctagaatc tcgttatttc cacaaaaaac
    16921 agcaacccaa aacggcaact taaaaccccg tcattcccgc gcaggcggga atctaggtct
    16981 gtcggttcag gaacttatcg gataaaacgg tttctccaac cctgcgttct agattcccac
    17041 tttcgtggga atgacgggat atgggtttcc gtgcggacgt gttcggattt ccgcctgcgc
    17101 gggaatgacg gcgacagatg ccc
//
LOCUS       DAWKQB010000031        16462 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.031, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000031 DAWKQB010000000
VERSION     DAWKQB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.031"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            209..1273
                     /locus_tag="WJL14_000756"
     CDS             209..1273
                     /locus_tag="WJL14_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ7655857.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIIHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1414..1737
                     /locus_tag="WJL14_000757"
     CDS             1414..1737
                     /locus_tag="WJL14_000757"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ7655858.1"
                     /translation="MGDSVLSAIQQTIIQRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKDPSQLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1809..2132
                     /locus_tag="WJL14_000758"
     CDS             1809..2132
                     /locus_tag="WJL14_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ7655859.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMICFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQMLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2180..2383
                     /gene="tatA"
                     /locus_tag="WJL14_000759"
     CDS             2180..2383
                     /gene="tatA"
                     /locus_tag="WJL14_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ7655860.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTNGKEAKKDDVIEHKKDEDKA"
     gene            2387..3073
                     /gene="tatB"
                     /locus_tag="WJL14_000760"
     CDS             2387..3073
                     /gene="tatB"
                     /locus_tag="WJL14_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ7655861.1"
                     /translation="MFDFGLGELILVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEGLRKAKQEFEAAAAQVRDSLKETDTDMQNSLHDISDGLKPWDKLP
                     EQRTPADFGVDEGGNPLPDAANTLSDGISDVMPSERSDTSAETLGDDRQTGSTAEPAE
                     TDKDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3088..3858
                     /gene="tatC"
                     /locus_tag="WJL14_000761"
     CDS             3088..3858
                     /gene="tatC"
                     /locus_tag="WJL14_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ7655862.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWMVVGILVCFFGMMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLVSLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKHARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPVKT"
     gene            3910..4518
                     /gene="maf"
                     /locus_tag="WJL14_000762"
     CDS             3910..4518
                     /gene="maf"
                     /locus_tag="WJL14_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ7655863.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYRVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPHSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGNAENRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4527..4880
                     /locus_tag="WJL14_000763"
     CDS             4527..4880
                     /locus_tag="WJL14_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ7655864.1"
                     /translation="MNRQTAYLLASFSLIALIILSLSWELWIAPLRLGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSSVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4870..6684
                     /locus_tag="WJL14_000764"
     CDS             4870..6684
                     /locus_tag="WJL14_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ7655865.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACRSLVAHYGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALTAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKHPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVHSFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKALKYRPYSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6874..7551
                     /locus_tag="WJL14_000765"
     CDS             6874..7551
                     /locus_tag="WJL14_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7655866.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVHSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7565..8971
                     /locus_tag="WJL14_000766"
     CDS             7565..8971
                     /locus_tag="WJL14_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ7655867.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIIVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9089..11515
                     /locus_tag="WJL14_000767"
     CDS             9089..11515
                     /locus_tag="WJL14_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ7655868.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLPAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTACCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTVIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTARDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11560..11805
                     /gene="rpsP"
                     /locus_tag="WJL14_000768"
     CDS             11560..11805
                     /gene="rpsP"
                     /locus_tag="WJL14_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ7655869.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11821..12330
                     /gene="rimM"
                     /locus_tag="WJL14_000769"
     CDS             11821..12330
                     /gene="rimM"
                     /locus_tag="WJL14_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ7655870.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNSELQVKFEGINDRDLAFSLHGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12330..13079
                     /gene="trmD"
                     /locus_tag="WJL14_000770"
     CDS             12330..13079
                     /gene="trmD"
                     /locus_tag="WJL14_000770"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ7655871.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13095..13460
                     /gene="rplS"
                     /locus_tag="WJL14_000771"
     CDS             13095..13460
                     /gene="rplS"
                     /locus_tag="WJL14_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ7655872.1"
                     /translation="MNLIQQLEQEEIARLNKDIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13778..14503
                     /locus_tag="WJL14_000772"
     CDS             13778..14503
                     /locus_tag="WJL14_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ7655873.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14539..15414
                     /locus_tag="WJL14_000773"
     CDS             14539..15414
                     /locus_tag="WJL14_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ7655874.1"
                     /translation="MNPYDLLITPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSTLTLLTLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15491..16417
                     /locus_tag="WJL14_000774"
     CDS             15491..16417
                     /locus_tag="WJL14_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ7655875.1"
                     /translation="MKHPKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLVLINGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALI
                     KADPEGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHI
                     EFIAPQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSG
                     KLYSDALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4291 a         5279 c         3597 g         3295 t
ORIGIN      
        1 ccgtctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
       61 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
      121 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
      181 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg ataaaggcga
      241 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcactgtcg gcatcaatgt
      301 cgcctggtgc ggcatctgcg gtactgacct gcacgaattc atggaaggcc cgattttcat
      361 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtaa cgatgggaca
      421 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggcca
      481 acacgtcgtg gtcgaaccct acatcatcca cgatgacgta ccgaccggcc cgggcagcaa
      541 ctaccacctc tccaaagata tgaactttat cggcttgggc ggctgcggcg gcggtctgtc
      601 cgaaaaaatc gccgtcaaac gccgttgggt gcatccgatt tccgacaaaa tcccgttgga
      661 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
      721 gaaagaaggc gatgtcgcat tggttggcgg tgcaggccca atcggtttgc tgttggctgc
      781 cgtactgaaa gccaaaggca tcaaagtcat cattaccgag ttgagtaaag cacgcaaaga
      841 caaagcgcgc gaatccggcg ttgccgacta catcctcgac ccgtccgaag tcgatgtcgt
      901 cgaagaagtg aaaaaactga ccaatggcga aggcgtggac gtggcattcg aatgtaccag
      961 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
     1021 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
     1081 ggatgtgcgc ggcaccatcg cttactgcaa cgaccacgcg gaaaccatca aactggtcga
     1141 agaaggcaaa atcaacctcg agcctttcat cacccagcgc atcaagctgg acgaactggt
     1201 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgaa
     1261 tccaaacctg taagcaaatt atatttgcaa cagcacaaat gccgtctgaa acgctcagac
     1321 ggcattttcc aacgaaacgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
     1381 tccgaacgaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
     1441 caacaaacca ttatacagcg caaatctgcc gatccgtccg aatcttacgt cgcacagctc
     1501 ctgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
     1561 atggcatcca aagacaaaga cccgtcccaa ctggtttacg aagttgccga cttatggttt
     1621 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtact ggacgaactt
     1681 gcgcgccgtc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
     1741 tatattacaa tccgcacttt cccacattca atccgtctga ccgcttttca gacggcatcg
     1801 gagctgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
     1861 ccgtctatga agacggggag atgatttgtt tcaaagacat caaccccgct gctccggttc
     1921 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgct cccgaacatc
     1981 agatgctgct gggcaaaatg atgttgaaag ttcccgaaat cgccaaagcg gcaggactgg
     2041 cagacggctt caaaaccctg atcaataccg gcaaaggcgg cggacaagag gtcttccacc
     2101 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca accccctaat
     2161 acttacttaa ggatacatca tgggcagttt ttctctgacg cactggatta tcgtactgat
     2221 tatcgtcgtt ttgatattcg gcaccaaaaa actgcgcaac gtcggcaaag acctcggcgg
     2281 cgcggttcat gacttcaaac aggggctgaa cgaaggcaca aacggcaaag aagccaaaaa
     2341 agacgatgta atcgaacaca aaaaagacga agacaaagcg taatttatgt ttgatttcgg
     2401 tttgggcgag ctgattttgg tcggcattat cgccctgatt gtcctcggcc ccgaacgcct
     2461 gcccgaggcc gcccgcaccg ccggacggct catcggcagg ctgcaacgct ttgtcggcag
     2521 tgtcaaacag gaatttgaca ctcaaatcga actggaagga ctgaggaagg caaagcagga
     2581 attcgaagct gccgctgctc aggttcgaga cagcctcaaa gaaaccgata cggatatgca
     2641 gaacagtctg cacgacattt ctgacggtct gaaaccttgg gacaaactgc ccgaacagcg
     2701 cacgcctgcc gatttcggtg tcgatgaagg cggcaacccc cttcctgatg cggcaaacac
     2761 cctatcagac ggcatttccg acgttatgcc gtctgaacgt tccgatactt ccgccgaaac
     2821 ccttggggac gacaggcaaa ccggcagtac agccgaaccc gcggaaaccg acaaagaccg
     2881 cgcatggcgg gaatacctga ctgcttctgc cgccgcaccc gtcgtacaga ccgtcgaagt
     2941 cagctatatc gatactgctg ttgaaacgcc tgttccgcac accacttccc tgcgcaaaca
     3001 ggcaataagc cgcaaacgcg gtttgcgtcc gaaacaccgc gccaaaccta aattgcgcgt
     3061 ccgtaaatca taaagagggc aaatccggtg tccgaaacac aaaacgaaca acccgtccaa
     3121 ccgcttgtcg aacacctcat cgagctgcgc cgccgcctga tgtggatggt tgtcggcatt
     3181 ctcgtctgct ttttcggcat gatgccgttt gcccaacaac tctatacttt tatcgccgac
     3241 ccgctgatgg caaacctgcc aaaagacacc agcatgattg cgaccgatgt catcgcaccg
     3301 tttttcgttc cggtcaaagt taccctgatg gcggcgtttt tggtttcact gccgcatacg
     3361 ctctaccaaa tctgggcatt cgtcgcgccc gcactctacc aaaacgaaaa acgcctgatt
     3421 acgccgctcg tcctctccag cgtcagcctg tttttcatcg gcatggcatt tgcctacttt
     3481 ctggttttcc ccgtcatttt caaattcctt gccggcgtaa cccccgtcgg agtcaatatg
     3541 gcgaccgaca tagacaaata cctctccttc atcttgggga tgtttgtcgc attcggtaca
     3601 acgtttgaag ttcccatcgt cgttatcctg ttaaccaaaa tcggcgtggt aacaaccgaa
     3661 caactcaaac atgcccgccc ctatgtcatt gtcggtgcgt ttgtcattgc cgcagtcatc
     3721 acaccgcccg acgttatttc acaaaccctg cttgccattc cgctgattct cttatacgaa
     3781 gcaggtattt ggttcggacg ctttttcacg ccacgttcag aacaggatgg cgacatacag
     3841 ccgcctgtaa aaacctgaca ctatgccgtc tgaagcccca cctcataccg ccacagttaa
     3901 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cacgcagaat ggaaatcctg
     3961 acgcagttgg gctatcgggt cgtcaagctg cctgccgaca tcaacgaaac ggtcagacag
     4021 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
     4081 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
     4141 tgcgtcgttt cagacggcat tatattgggc aaaccccact cccaagccga agcaatcgaa
     4201 tttttaaacc gattgtccgg caaacaacat accgtcctga ctgccgtctg cattcattat
     4261 cgcggcaatg cggaaaaccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
     4321 gaagaaattt ccgcctatgt gcaaagcggc gaaccgatgg acaaagccgg tgcctacgcc
     4381 gtacaaggca taggcggcat ctttatccaa tctatcgaag gcagcttcag cggcattatg
     4441 ggactgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg ctcccccttg
     4501 tccgccctta aaccgtaaag accgccgtga acagacaaac cgcttacctc cttgcctctt
     4561 tcagcctgat cgcactgata atcctgtccc tttcctggga actgtggatt gcaccgttgc
     4621 gcctgggcgg ctcgtggctc gcgctcaaag ccctccccct ctgtctgcca ctttcaggta
     4681 tcttgaaaaa gaaaatctat acctaccaat acagctccat gctggttctg atttactttg
     4741 ccgaagccgt catgcgcctg ttcgatgcct atccggcaga acagatttgc gccgcgcttt
     4801 cctcagtatt cagcatcatc ttcttcatat cctgcctgtc gttcgtcaaa caatacaagg
     4861 aaacaaacaa tgcccgctga aacgaccgta tccggcgcgc accccgccgc caaactgccg
     4921 atttacatcc tgccctgctt cctttggata ggcatcgtcc cctttacctt tgcgctcagg
     4981 ctgcaaccgt cgcccgactt ttaccacgat gccgccgccg cagccggcct gattgtcctg
     5041 ttgttcctca cggcaggaaa aaagctgttt gatgtcaaaa tcccacctat cagcttcctt
     5101 ctgtttgcaa tggcggcgtt ttggtatctt caggcacgcc tgatgaacct gatttacccc
     5161 ggtatgaacg acatcgtctc ttggattttc atcttgctcg ccgtcagcgc gtgggcctgc
     5221 cggagcttgg tcgcacacta cggacaagaa cgcatcgtta ccctgtttgc ctggtcactg
     5281 cttatcggct ccctgcttca atcctgtatc gtcgtcatcc agtttgccgg ctgggaagac
     5341 acccccctgc ttcaaaacat catcgtttac agcgggcaag gcgtaatcgg acacatcggg
     5401 cagcgcaaca acctcggaca ctacctcatg tggggcatac tcgccgccgc ctacctcaac
     5461 ggacaacgaa aaatccccgc cgccctcggc gcaatctgcc tgattatgca gaccgccgtt
     5521 ttaggtttgg tcaattcgcg caccatcttg acctacatag ccgccatcgc cctcatcctt
     5581 cccttctggt atttccgttc ggacaaatcc aacaggcgga cgatgctcgg catagccgca
     5641 gccgtattcc ttaccgcgct gttccaattt tccatgaaca ccattctgga aacctttacc
     5701 ggcatccgct acgaaactgc cgtcgaacgc gtcgccaacg gcggtttcac agacttgccg
     5761 cgccaaatcg aatggaataa agccctcacc gccttccagt ccgccccgat attcgggcac
     5821 ggctggaaca gttttgccca acaaaccttc ctcatcaatg ccgaacagca caacatacac
     5881 gacaacctcc tcagcaactt gttcacccat tcccacaaca tcgtcctcca actccttgca
     5941 gagatgggaa tcagcggcac gcttctggtt gccgcaaccc tgctgacggg cattgccggg
     6001 ctgctgaaac accccctgac ccctgcatcg cttttcctga tctgcacgct tgccgtcagt
     6061 atgtgccaca gtatgctcga atatcctttg tggtatgtct atttcctcat ccccttcgga
     6121 ctgatgctct tcctgtcccc cgcagaggct tcagacggca tcgccttcaa aaaagccgcc
     6181 aacctcggca tactgaccgc ctccgccgcc atattcgcag gattgctgca cttggactgg
     6241 acatacaccc ggctggttca ctccttttcc cccgccactg acgacagtgc caaaaccctc
     6301 aaccgaaaaa tcaacgaact gcgctatatt tctgcaaaca gtccgatgct gtccttttat
     6361 gccgacttct ccctcgtaaa cttcgccctg ccggaatacc ccgaaaccca gacttgggcg
     6421 gaagaagcaa ccctcaaagc actaaaatac cgcccctact ccgccaccta ccgcatcgcc
     6481 ctctacctga tgcggcaagg caaagttgca gaagcaaaac aatggatgcg agcgacacag
     6541 tcctattacc cctacctgat gccccgatac gccgacgaaa tccgcaaact gcccgtatgg
     6601 gcgccgctgc tgcccgaact gctcaaagac tgcaaagcct tcgccgccgc gcccggtcat
     6661 ccggaagcaa aaccctgcaa atgacccgcg ccggcggatg cgcatgccgt ctggaatata
     6721 aagacccttg caaagcattg caaaaaacag caaacacgca gaaaaatacg ctatcaaaaa
     6781 acattgcagc cgtgttaaga taaaccgtta aacacttgtt tcatgcccgt cccaaacccg
     6841 gcgggcgcat accaccacgg aaaaggaaac accatgagcc gcgtattact cgtagatgac
     6901 gatgccctgc tgaccgaact gctgaccgaa tacctgagcg ccgaaggtct gaacgtccac
     6961 agcgttcccg acggggaagc aggcgtacag gaaatcctga gcgggcaata cgatgtagtc
     7021 gtattggatt ccatgatgcc caaaatgaac ggcttggatg tcttgaaaaa cgtacgcgcc
     7081 cgaagcaccg tccccatcat catgctgacc gccaaaggcg acgacatcga ccgaatcatc
     7141 ggcttggaaa tgggcgcgga cgactatgtc cccaaaccct gcacaccacg cgaactcttg
     7201 gcacgcatca atgccatcct gcgccgcgca caacacagcg gcgaacagaa caacgcaccc
     7261 aacagcatct ccgtcagcga tgtcgtcctg taccccgcca aacgccaggc atccgtcaaa
     7321 gacatgccgc tcgaactgac cagcaccgaa ttcaacctgc tcgaagtcct gatgcgccat
     7381 gccggacagg tagtcagcaa agaaaccctg tccgtcgaag cactcgaccg caagctggca
     7441 aaattcgacc gcagtatcga cgtacacatc tccagcatcc gccacaagtt gggcgatgcc
     7501 tctctgattc aaaccgtacg cggcttgggc tacctgtttg tcaaaaactg aaataaacag
     7561 ataaatgaaa ctgttccaac gcatcttcgc cacattttgc gcggttatcg tctgtgcaat
     7621 ctttgtggcg agtttttctt tctggctggt gcagaacacc cttgccgaaa accagttcaa
     7681 ccaacgccgc accatcgaaa ccactttgat gggcagcatc atttccgcat tccgggcacg
     7741 cggggacgcg ggtgcgcgcg aaatcctgac ggaatggaaa gacagccccg tctcatcggg
     7801 cgtgtacgtt atacagggcg acgagaaaaa agatatcctg aaccggtata tcgacagcta
     7861 caccatcgaa cgcgcccggc ttttcgccgc cggacacccg cattccaacc tcgtccatat
     7921 cgaatacgac cgcttcggcg aagaatacct gttcttcacc aaagactggg acaaactcca
     7981 agcccgccgc ctgcccagcc ccctgttgat ccccggcctg ccgctcgccc cgatttggca
     8041 cgaactcatc atattgtcct tcatcatcat cgtcggactg ctgatggcgt acatcctcgc
     8101 cggcaacatt gccaaaccca tcagaatctt aggcaacggc atggacaggg tggcaaacgg
     8161 agaacttgaa acccgtatct cccaacaggt cgacgaccgc gacgacgaat tgtcccatct
     8221 tgccatccaa ttcgacaaaa tggtggaaaa actcgaaaaa ctcgttgcca aagaacgcca
     8281 cctgctccat cacgtctccc atgaaatgcg ttctcccctt gcgcgcatgc aggcaattgt
     8341 cggactgatt caggcgcagc cccaaaaaca ggagcaatat ctcaaacggc tggaaggcga
     8401 actgacccgc atggatacgc tggccgggga actgttaacc ctgtcccgtc tcgaaacttc
     8461 caatatggct ttggaaaaag aaagcctgaa actcctgccc ttcctgggca acctggtaga
     8521 agacaatcaa agcattgccc agaaaaacgg acaaacggtt accctgtctg ccgacggaaa
     8581 aatccccgaa aacacaacca tccttgccaa cgaaagctac ctgtaccgcg ccttcgacaa
     8641 cgtcatccgc aacgccgtca actacagtcc cgaaggcagc accatcctga tcaacatcgg
     8701 acaagaccac aaacactgga taatcgacgt taccgacaac ggccccggcg tggacgaaat
     8761 gcagctcccg cacatcttca ccgctttcta ccgtgcagac tccagtgcca acaaacccgg
     8821 aacaggactg gggcttgcat tgacccaaca tattattgaa cagcactgcg gcaaaatcat
     8881 cgccgaaaac atcaaaccga acggtctgcg gatgcgcttt atcctgccca agaaaaaaac
     8941 cggttccaaa acagaaaaaa gtgcgaactg accataatac cgtctgaacc ggcttcagac
     9001 ggcattgcac aaacagttac cccaaaacaa acacacagcg ggtgcatctc caaaaatcac
     9061 gcacataccg tccgcaaagg aatatatcat gtcggcacaa accgatccgg gctacttttt
     9121 catgcccaac cacattattc tgataggcgc gagcgaacgt ccgtacagcc tgggcgaacg
     9181 cgtactcagc aacctgctga gtacgccctt tcaaggaaaa atcacccccg taaacccgcg
     9241 ccaccacacc atagctgggc ttcccgccta caccagcctc aacaaaatcc ccggcaatgc
     9301 agacctgatt attgccgtta ccctacccga cagttacgac accctcttca aaacctgccg
     9361 caaaaagcag ctccgacaca tcatcctcat acaggactgg gacagcctgc ctgccgcaga
     9421 actgcacacc gccgaaactg ccatccgcaa acaccacggc aacggactca acatcaccgc
     9481 ctgcaccacc gcaggcatcc aactgccctc actcggactt aacatcagta cccaagacgg
     9541 atacgccgca ggccataccg ccatactgac cggcaatgcc gccgtcagcc gccaaatcga
     9601 caacatcctg aacaaactcc gtcaaggcac atcccgccac atcagcctgc atcccggcat
     9661 cagccccatc acatccgccg attggctcaa ccgcttcgga cacagcctgc acaccaaaac
     9721 cgccgtccta caccacaacc ctgaagagga tcagcgcaaa ctgttcagcg caatccgcca
     9781 atttacccgc catacgccgc tgattctcca catcacctgc cgcacgacag aaaccgaccg
     9841 tgccgtactg cactgcctcg cccgacactg caacttcctc atcagtttca acgccgacga
     9901 cctcgaagcc gcactgcgcg cccaactgtc cgaccttcca cccctgtccc gactcgacat
     9961 cctgtccgac acgcctgccg aatggctgaa cgcgcacgcg ccaaaaaccc tcgccctcca
    10021 ctttcccaac cttccccacc acatccgcaa cggacacctg accggcacac ccacaccctc
    10081 aatctgccac gacatcgcct cacgtcagct tgcccgcccc gacacccaag ccgtcctgac
    10141 catcctcagc ccctccggac acgaggatta caaaaaaaca gcacgcgccc ttatccgcct
    10201 gtccgaacag accgccaaac ccctgctcgt cagcagcccc ttttcagacg gcataacaca
    10261 tttcgacacc cccactcagg caatccgcac cctttcctac cgcaacaccg ccgccgccct
    10321 gaaacaggca cagctcgaca ttgcaccgcc gcaaccgtgc cgtctgaaaa caccgcaacc
    10381 ccaaaacatc aaaaaagccc tcgaagccgc cgaccccgcc ctaatcgccg aagccctgca
    10441 cctccccccc taccggcaca ctacccataa cgccgtccaa ttccaattcg gcagccaccc
    10501 cctctatggc gacatcctga ccgcatgctg caacggacaa accactgccg tactcccgcc
    10561 gtttaccacg ctcgacagcc gccaccttgc ccgctttgcc gaactcgacg gcacacaaac
    10621 cctcgaccag ttcctgcaca cactgaccgt cattcccgaa taccgccaac acattctcgg
    10681 catcaccctc aacctcaacg gcggacaata cagcagcgat ttcatcttaa agacacccga
    10741 aacacacgac acgcccaaac gcaagaacac aggcaaagcc gcccaaaccc tcgaacatgc
    10801 cgccgcaaaa atgcagagtg ccgccgcata cctgaaacac aaaaacccga cagccgccga
    10861 atttctccgc cacacaagcg aagccgccgc agaactgctc ggcagcaaaa ccgaaaccgg
    10921 agcagccgta cccaacgtac ttgcccccta tcccgcagca cacctcaaaa cactgtccct
    10981 aaaaaacaac acgaccgtta ccattacccc ccttttgccc gaagacgcag aagccaaaca
    11041 gcagttcgtc cgcagcctca gccccgaagc acggtacaca cgcttcatga cccacaccaa
    11101 cgaactgccc gcagccacgt tggcacgcct gtgcaacccc gactaccact gtgaagccgc
    11161 atggacggca agggatgccg acagcaacat cgtcgccgtc gtccgccaca gccgcctgaa
    11221 ccgcaacgaa tgcgaattcg gcatcacact ggcggaacat atgcgcggca gcggactggc
    11281 acagaaaatg atggaactca tcatccaaac cgccgcacag caaggctacc ggactatgag
    11341 tgccgacatt ctcaaaacca atactcccat gatcaaactt gctgaaaaat caggatttac
    11401 cctcaaggaa tcggacaccg aaaaaaacct gtaccgcgca tatctgaacc ttgcggcaga
    11461 caaaacaaca gaaaaaacaa ataaaaactt gcgcaccgac cacaaaataa cctaaaatcg
    11521 gcagtttcca tatatccgat tttctcaaaa ggactcaaaa tggtagttat ccgtttggca
    11581 cgcggcggct cgaaacaccg ccctttctac aacgtcatcg ttactgactc acgcagccgc
    11641 cgcgacggcc gcttcatcga acgcgtaggc ttctacaacc ccgtagccaa cgaaaaacaa
    11701 gagcgcgtcc gcctcaacgc agaccgcctg aaccactgga ttgcacaagg cgcgcaagtc
    11761 agcgactccg ttgcaaaact gattaaagaa caaaaagccg cctaatccac atttgccgcc
    11821 atgacagaca ctcaaaaccg ggtagccatg ggctacatca aaggcgtatt cggcataaaa
    11881 ggctggttga aaattgccgc caacaccgaa tattccgaca gccttttgga ctaccccgag
    11941 tggcatttgg tcaaggacgg caaaaccatc agcgttaccc ttgaagccgg aaaagtcgtc
    12001 aacagcgaac tccaagtcaa attcgaaggc ataaacgacc gcgacttggc attctcattg
    12061 cacggttaca ccatcgaaat accccgtgaa gcattcgccc cgacagaaga agacgaatac
    12121 tactggacag acttggtcgg catgaccgtc gtcaacaaag accataccgt tttaggcaag
    12181 gtaagcaacc tgatggaaac cggcgcaaac gacgtattga tgattgacgg agaacacggg
    12241 cagattctga ttccgttcgt ttcccaatat atcgaaaccg tcgataccgg cagcaagatt
    12301 attactgccg actggggttt ggactactga tgcttatcca ggcagttacc attttccccg
    12361 aaatgttcga cagcattacc cgctacggcg taacgggacg cgcgaacaga cagggaatct
    12421 ggcagtttga agcagtcaat ccccgaaagt ttgccgacaa cagattgggc tacatcgacg
    12481 accgcccgtt cggcggcggc ccgggaatga ttatgatggc tccgccgctt catgcagcaa
    12541 tagaacacgc caaagcacaa tcttcccaag ctgcaaaagt catttacctc agcccccaag
    12601 gaaaaccgct gacacaccca aaagcggcag aactggcaga acttccgcat ctgattctgc
    12661 tgtgcggacg ctatgaggga atagacgaaa gactgctgca aaccagcgtc gatgaagaaa
    12721 tcagcatcgg agacttcgtt gtttccggcg gagagcttcc cgccatgatg ctgatggatg
    12781 cggtattgag gctcgtaccc ggcgtattgg gcgatatgca gtctgccgaa caagattcgt
    12841 tctcaagcgg cattttggac tgcccccact acaccaaacc cttagaattt caaggcatgg
    12901 ctgttccgga agtattacgt tccggcaatc atggcttgat agcggaatgg cggttggaac
    12961 aatcgctgcg ccgcaccttg gagcgcagac ccgatctttt ggaaaagcgc gttttaatcc
    13021 caaaggaatc ccgcctctta gaaaccatcc ggcaagagca acgggaaatc caatcataat
    13081 ttaggaaaaa aacaatgaac ctgattcaac agctcgagca agaagaaatt gcccgtctga
    13141 acaaagacat ccccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtag
    13201 agggtacccg tagccgtctg caagcctacg aaggcgttgt cattgcacgc cgcaaccgtg
    13261 gtctgaacag caacttcatc gtccgtaaaa tttccagcgg cgaaggtgtg gaacgtactt
    13321 tccaactgta ctcccctacc gttgagaaaa tcgaagtcaa acgccgtggc gatgtccgcc
    13381 gtgccaaact ttactatctg cgcggtctga ccggcaaagc tgcacgcatt aaagaaaaat
    13441 tgcctgcacg caaaggttaa taagcgattt cccccaatgc cgtctgaact ttcagacggc
    13501 atttcttatt gctgcgatgc cggcaagtca acgatatgcc tgcccctcat ccaccttgat
    13561 taacaatcac tcccgcctat tcaagcataa tttacagacc aaacgttata cagtatcata
    13621 tcagccatat aacgataaac gctttaccgc cttccctttc aaaaccgcaa atcatcatga
    13681 caccttccct tttactatca ggattgactt tccgcctcat ccttgccctg attgccgtat
    13741 cccttttatg gggcgtttac ttttgggcag tatccgcatg agcatcattg tcgaaaacct
    13801 gaccgtcagc taccgccgcc gacctgccgt gcaccatgtg gacattactt ttgaagaaca
    13861 tagtatgtgg gcggttttcg gtcccaacgg cgcagggaaa tccacctttc tcaaatcctt
    13921 aatgggattg cagcctatcg atacgggcag catccggctg gacggattga cccgtcagaa
    13981 cattgcctac cttccccagc agtccgacat cgaccgctcg cagcctatga ccgttttcga
    14041 cttggcggca atggggctat ggtatgaaat cggttttttc agaggaataa atgccgttca
    14101 aaaacaacgc gttcacgaat ctttggaacg cgtcgggatg cggcagtttg ccggccgcca
    14161 aatcgcccac ctctcaaacg gacaattcca gcgtgtcctt tttgcccgaa tgctggttca
    14221 aaacgccaaa ttcctacttc tagacgaacc tttcaacgct gttgatgcac ggacgaccta
    14281 cgaacttctc gacgtattgc agaaatgcca ttgcggcgga cacgccatca tcgccgtact
    14341 gcacgattac gaacaagtcc gtgcctactt tcccaatacc ctgctgctcg cccgcgaaaa
    14401 aattgcggca ggcgcaaccg agaccattct gacagaaccc tacctcgccc aagccaacgc
    14461 caaaatgcag caacaggaaa gccccgagtg gtgcgcctca taaatgccgt ctgaaaccat
    14521 gtccccgtac caaacactat gaacccctac gacctgctta tcacaccctt tgcagaattc
    14581 gactttatgc gctacgccct cgcatccgtc ttctgcctgt ccctcagtgc cgcacccgtc
    14641 ggcgtattcc tcgtcatgcg ccgtatgagc ctgataggcg acgcattgag ccacgccgtc
    14701 ctgcccggtg ccgccgtcgg ctacatgttt gccggcttaa gcctgcccgc catgggtttg
    14761 ggcggcgtag ccgcaggtat gctgatggca ctgcttgccg gactcgtcag ccgcttcacc
    14821 accctgaaag aagatgccaa ctttgccgcc ttttatctca gcagcctcgc catcggtgta
    14881 gtcctcgtca gcaaaaacgg cagcagcgtc gatttgctcc acctcctttt cggctccgta
    14941 cttgccgtcg atattcctgc cctgcaactc atcgccgccg tatccaccct cacactgctt
    15001 acccttgccg tcatctaccg cccgctcgta ctcgaaagca tcgaccccct gtttctcaaa
    15061 tctgtcggcg gcaaaggcgg gctttggcac gtcctctttc tcgtcctggt cgtcatgaac
    15121 ctcgtatccg gctttcaagc cctcggcaca ctcatgtccg tcggacttat gatgctgcca
    15181 gccattaccg cccgcctatg ggcgaagcac atgggcgcac tcatcctcct atccgttctg
    15241 acagccctgc tgtgcggctt gagcggactg ctcatttcct accacatcga aattccttcc
    15301 ggtcccgcca tcatcctctg ttgcagcgtc ctttatctct tttccgtcat actcggcaaa
    15361 gaaggcggca ttctgaccaa atggctcaaa aaccaccgcc accacaccac ctgaccccgc
    15421 aagccgcttg aacaaacagg cggcttgttt taccataaca gatgttacgt tatatcacag
    15481 gagaacaatt atgaaacacc ccaaactcac ccttatcgcc gcattgctga ccactgccgc
    15541 aactgccgcc cccctgccgg ttgtaaccag cttcagcatt ttaggcgacg tagccaaaca
    15601 aatcggcgga gagcgcgtat ccatacaaag tttggtcgga gccaaccaag atacgcacgc
    15661 ctatcatatg accagcggcg acattaaaaa aatccgcagt gcaaaactcg tcctgattaa
    15721 cggcttagga cttgaagctg ccgacatcca acgtgccgtc aaacagagca aagtatccta
    15781 tgccgaagcg accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca
    15841 cgaccacgat catgaccacg accatgacca cgaaggacac caccacgacc acggcgaata
    15901 tgacccccac gtctggaacg accccgtcct tatgtccgcc tatgcccaaa acgtcgccga
    15961 agccctgata aaggccgacc ccgaaggcaa agtttattat caacaacgct tgggcaacta
    16021 ccaaatgcag ctcaaaaaac tgcacagtga cgcacaagcc gcatttaatg ccgtccctgc
    16081 cgccaaacgc aaagtcctga ccgggcacga tgccttttcc tatatgggca aacgttacca
    16141 tatcgaattc atcgccccac aaggtgtgag cagcgaagcc gagccttcag ccaaacaagt
    16201 cgccgccatc atccgacaaa tcaaacgcga aggcatcaaa gccgtattta ccgaaaatat
    16261 caaagacacc cgcatggttg accgcatcgc caaagaaacc ggtgtcaacg tcagcggcaa
    16321 actgtattcc gacgcactcg gcaacgcacc cgcagacacc tacatcggca tgtaccgcca
    16381 caacatcaaa gccttaacca acgcgatgaa gcaataaaac cgtttgattg gtttgaattt
    16441 gaaaaggcaa aaggccgtct ga
//
LOCUS       DAWKQB010000032        16247 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.032, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000032 DAWKQB010000000
VERSION     DAWKQB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.032"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..108
                     /locus_tag="WJL14_000775"
     CDS             <1..108
                     /locus_tag="WJL14_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7655876.1"
                     /translation="TVELIAPIAMEEGLRFAIREGGRTVGAGVVSSVIA"
     gene            115..190
                     /locus_tag="WJL14_000776"
     tRNA            115..190
                     /locus_tag="WJL14_000776"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:148..150,aa:Trp,seq:cca)
     gene            305..583
                     /gene="secE"
                     /locus_tag="WJL14_000777"
     CDS             305..583
                     /gene="secE"
                     /locus_tag="WJL14_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ7655877.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWFEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            585..1121
                     /gene="nusG"
                     /locus_tag="WJL14_000778"
     CDS             585..1121
                     /gene="nusG"
                     /locus_tag="WJL14_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ7655878.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1222..1656
                     /gene="rplK"
                     /locus_tag="WJL14_000779"
     CDS             1222..1656
                     /gene="rplK"
                     /locus_tag="WJL14_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ7655879.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1656..2351
                     /gene="rplA"
                     /locus_tag="WJL14_000780"
     CDS             1656..2351
                     /gene="rplA"
                     /locus_tag="WJL14_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ7655880.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2581..3081
                     /gene="rplJ"
                     /locus_tag="WJL14_000781"
     CDS             2581..3081
                     /gene="rplJ"
                     /locus_tag="WJL14_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ7655881.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3139..3510
                     /gene="rplL"
                     /locus_tag="WJL14_000782"
     CDS             3139..3510
                     /gene="rplL"
                     /locus_tag="WJL14_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ7655882.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3701..7879
                     /gene="rpoB"
                     /locus_tag="WJL14_000783"
     CDS             3701..7879
                     /gene="rpoB"
                     /locus_tag="WJL14_000783"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ7655883.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVVRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            8031..12206
                     /gene="rpoC"
                     /locus_tag="WJL14_000784"
     CDS             8031..12206
                     /gene="rpoC"
                     /locus_tag="WJL14_000784"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ7655884.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12497..12754
                     /locus_tag="WJL14_000785"
     CDS             12497..12754
                     /locus_tag="WJL14_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655885.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGDRMGMPINIG"
     gene            12751..12900
                     /locus_tag="WJL14_000786"
     CDS             12751..12900
                     /locus_tag="WJL14_000786"
                     /inference="COORDINATES: protein motif:HMM:NF016462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7655886.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSSKEWFVS
                     IEKTY"
     gene            13079..13450
                     /gene="rpsL"
                     /locus_tag="WJL14_000787"
     CDS             13079..13450
                     /gene="rpsL"
                     /locus_tag="WJL14_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ7655887.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13568..14038
                     /gene="rpsG"
                     /locus_tag="WJL14_000788"
     CDS             13568..14038
                     /gene="rpsG"
                     /locus_tag="WJL14_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ7655888.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            14057..16162
                     /gene="fusA"
                     /locus_tag="WJL14_000789"
     CDS             14057..16162
                     /gene="fusA"
                     /locus_tag="WJL14_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ7655889.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEVVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4309 a         3608 c         4217 g         4113 t
ORIGIN      
        1 accgtagaac tgattgcgcc tatcgctatg gaagaaggcc tgcgctttgc gattcgcgaa
       61 ggcggtcgta ccgtgggtgc cggcgtggtt tcttctgtta tcgcttaagt ttagaggcca
      121 atagctcaat tggtagagta tcggtctcca aaaccgaggg ttgggggttc gagaccctct
      181 tggcctgcca aataaaaaat taaccggcct tgtgtcggtt aatttttttg tatttgttat
      241 ttagtaaact ctcttgccat ttacatggat tgagaataga cagatgctat gatggataaa
      301 taatatgaca gaacatacgc ctgaaaaaaa gaacgttaaa gtggatcaac tggttgttca
      361 agataaagaa tctgcatcta attccggtaa ggaagggttt tttgcatatt tctcaaattc
      421 ttggttcgaa ttcaaaaagg tggtttggcc taagcgtgaa gatgctgtca gaatgactgt
      481 atttgttata gtgtttgttg ctgtgctttc tatatttatc tatgcggcag atacagcaat
      541 ttcgtggtta ttttttgatg tattgctgag aagggaaggt tgagatgtcg aaaaaatggt
      601 atgttgtaca ggcgtattcg gggtttgaga agaatgtcca acgaatattg gaagagcgca
      661 ttgcccgtga ggagatggga gattatttcg gacaaattct ggtgcctgta gagaaagttg
      721 ttgatatccg caatggtcgt aagactatta gtgaaagaaa gtcatatcct ggttatgtgc
      781 tagttgagat ggaaatgaca gatgactctt ggcatcttgt aaaaagcacc ccccgtgttt
      841 ccggttttat tggagggagg gctaatagac ctacgccgat tagtcagaga gaggctgaaa
      901 ttattttaca gcaggttcag accggcatag agaagccgaa accaaaagtt gaatttgagg
      961 tcggtcaaca ggttcgtgta aatgaagggc cgtttgcgga ttttaacggg gtggttgagg
     1021 aggtcaatta tgaacggaat aagttacgcg tgtctgttca gatatttggt agagaaacac
     1081 ccgttgagct ggagttcagc caggttgaaa agattaactg atttttatac ttgaaaaaaa
     1141 agcaataaga ggatagaatc aaaaattaac ttggggagcg gaaatggttc cgcgtcttac
     1201 ccgtttttag gagttcgtta agtggcaaag aaaattatcg gctatattaa actgcaaatt
     1261 cctgcaggta aagccaatcc atctcctccg gttggtcctg ctttgggtca gcgcggtttg
     1321 aatattatgg aattttgtaa ggcatttaat gctgcaaccc aaggtatgga gcctggctta
     1381 ccgattccgg ttgtgattac tgcatttgca gataaatcat tcacatttgt gatgaaaacc
     1441 ccgccagctt ctatcttgtt gaaaaaggct gccggtttgc aaaaaggtag ttctaatcct
     1501 ctgaccaaca aagtgggtaa attgacccgt gcccagttgg aagaaattgc taaaactaaa
     1561 gagcctgatt tgactgctgc tgacttggat gcggctgtcc gtactatagc aggttctgct
     1621 cgctcaatgg gcttggatgt ggagggtgtt gtataatggc taaagtatct aaacgcttga
     1681 aagctcttcg ctcttctgtg gaagccaata aattatatgc aattgatgaa gcaattgctt
     1741 tggtaaaaaa agcagcgact gctaaatttg acgagtctgt tgacgtatct ttcaacttgg
     1801 gcgttgatcc gcgtaaatct gaccaagtta tccgtggttc agtcgttctg cctaaaggca
     1861 ccggtaagat aactcgcgta gctgtattta ctcaaggtgc aaatgcagaa gctgctaaag
     1921 aagctggtgc agatatcgtc ggtttcgaag atttggctgc tgaaatcaaa gcaggcaatc
     1981 tgaactttga tgtcgttatt gcttctcccg atgcaatgcg tattgttggt cagttgggta
     2041 ctattttggg tcctcgaggc ttgatgccaa accctaaagt aggtactgtt actcctaacg
     2101 ttgctgaagc agtaaaaaat gcaaaagcag gtcaagtaca ataccgtaca gataaagcag
     2161 gtatcgttca tgcaacgatt ggtcgtgctt ctttcgctga agctgacttg aaagagaact
     2221 ttgatgcgtt gctggatgct atcgttaaag ccaagcctgc tgccgctaaa ggtcagtatc
     2281 tgaaaaaagt tgctgtgtct agcaccatgg gtttgggtat tcgcgttgat acatcaagcg
     2341 taaataacta atcttaagga attttcaagc agtttggttt tctgggctgc ttgaatttgg
     2401 gctacttaaa attaagtaga tgtccaagac cgtagggatc gtaagattta atcgtaactg
     2461 ccctacgcag acggtagtcc tgaaacacat tgcaagattg cttgtaagat gtctttttag
     2521 gttaccgcgc tggtgggata tcgttttggt atcctgttta taaacagtgg gaggtagacc
     2581 ttgagtctca atattgaaac caagaaagtg gcggtcgagg aaattagcgc ggcaattgct
     2641 aatgctcaaa ccctcgtagt cgctgaatat cgcggtatca gtgtttccag tatgactgag
     2701 cttcgtgcga atgcacgtaa agaaggcgtt tatttgcgcg ttctgaaaaa tactttggct
     2761 cgtcgtgcag tgcaaggtac ttcatttgca gaattggccg atcaaatggt tggtccgttg
     2821 gtttacgctg cttctgaaga tgctgttgct gctgctaaag tgttgcacca attcgcgaaa
     2881 aaagatgaca aaattgtcgt taaagccggt tcttacaatg gcgaagtaat gaatgctgct
     2941 caggttgctg agttggcttc tattccgagc cgcgaagagc tgttgtccaa actgttgttc
     3001 gttatgcaag ctcctgtatc gggctttgcg cgcggtttgg ctgctttggc agagaaaaaa
     3061 gccggcgaag aagccgctta atcgattttg tttctgttaa tcaattattt tttaatacaa
     3121 tatttggagt aaaatagcat ggctattact aaagaagaca ttttggaagc agttggttct
     3181 ttgaccgtaa tggaattgaa cgacttggtt aaagcttttg aagaaaaatt cggtgtttct
     3241 gctgctgctg ttgcagttgc aggtcctgct ggtgccggtg ctgccgatgc tgaagaaaaa
     3301 accgaatttg atgtcgtttt ggcttctgcc ggtgatcaaa aagtcggcgt gattaaagtt
     3361 gtccgtgcaa ttaccggttt gggtctgaaa gaagctaaag acatcgttga cggtgcacct
     3421 aaaaccatta aagagggtgt ttctaaagct gaagccgaag acatccaaaa acaactggaa
     3481 gaagccggcg ctaaagtcga aatcaaataa tttgatgctt cttatgaagg ctggcagttt
     3541 tctgccagcc ttattttgct tcttaaaata agcattaagt attgtttaca tttatttgca
     3601 tagtttttat caaatcattg caaataaatg taaacatcag attgatgcgt gccgttgctt
     3661 tcagacggcc tattattgaa aattactttt cggagtgtat atgagctatt cgtttaccga
     3721 gaaaaaacgt atccgtaaga gttttgcaaa gcgggaaaat gttttggaag ttcctttctt
     3781 gctggcaacc caaattgatt cttatgcgaa gtttttgcag ctggaaaatg cttttgacaa
     3841 acgtaccgat gacggtctgc aggcggcatt taattctatt ttcccgattg tgagccataa
     3901 cggttatgcg cgattggagt ttgtgcatta cacattgggc gagcctttgt tcgatattcc
     3961 cgaatgtcag ttgcgcggaa tcacttatgc agcccccttg cgcgcgcgta tccgtttggt
     4021 gattttggat aaggaagcat ctaaaccgac ggtaaaagaa gttcgtgaaa acgaagtgta
     4081 tatgggcgaa attccgttga tgaccccgag cggttctttt gtgattaacg gcacagagcg
     4141 tgtgattgtt tcccagttgc accgttcgcc cggcgtgttt ttcgagcatg acaaaggtaa
     4201 gacgcactct tccggcaaat tgttattctc cgcccgcatc attccctacc gtggttcatg
     4261 gttggatttt gaatttgatc cgaaagattt gctgtatttc cgtatcgacc gccgccgtaa
     4321 aatgccggta acgattttgt tgaaggcttt gggctacaac aatgagcaaa tcttggatat
     4381 tttctacgac aaagaaacgt tctatttgtc ttcaaacggt gttcaaaccg atttggtcgc
     4441 aggccgtctg aaaggcgaaa ctgccaaggt cgatatcttg gataaagaag gcaatgtatt
     4501 ggttgccaaa ggtaagcgca ttactgcgaa aaatatccgt gatattacca atgcaggcct
     4561 gacccgtttg gatgtagaac cagaaagcct gctgggcaaa gcattggctg ccgacctgat
     4621 tgattcggaa accggcgagg tattggcttc tgccaatgat gaaattacag aagagttgtt
     4681 ggccaaattt gatatcaacg gcgtaaaaga aattacgacc ctttacatca atgagttgga
     4741 tcagggtgct tatatctcca ataccttgcg tacggatgag actgccggcc ggcaggcggc
     4801 gcgtgttgcg atttaccgta tgatgcgtcc gggcgaaccg cccactgaag aggcggtcga
     4861 gcaattgttt aaccgcttgt tcttcagtga agacagctac gatttgtccc gcgtaggccg
     4921 tatgaaattt aatacgcgca catacgaaca aaaactgtcc gaagcccaac aaaactcttg
     4981 gtacggccgc ctgctgaacg aaacgtttgc cggtgctgcc gacaaaggcg gttatgtcct
     5041 gagcgtcgaa gatattgtcg cctcgattgc gactttggtc gagttgcgta acggccatgg
     5101 cgaagtggac gatatcgatc acttgggcaa ccgccgagta cgttcggtag gcgagctgac
     5161 tgaaaaccaa ttccgtagcg gtttggcccg tgtggaacgt gccgtaaaag aacgtttgaa
     5221 tcaggcggaa tcagaaaact tgatgccgca cgatttgatt aatgcaaaac ctgtttctgc
     5281 tgctattaaa gaattcttcg gctccagcca attgagtcag tttatggatc agaccaatcc
     5341 cttgtctgaa gtaacccata aacgccgcgt atccgcattg ggtccgggcg gtttgacccg
     5401 cgaacgtgca ggatttgagg tgcgggacgt gcatccgacc cactacggcc gcgtatgtcc
     5461 gattgaaacg cctgaaggtc cgaacatcgg tttgatcaac tcactgtccg tttatgcgcg
     5521 caccaatgat tacggtttct tggaaacgcc ttaccgccgc gttatcgacg gcaaagtaac
     5581 cgaggaaatc gattacttgt ctgccatcga agaaggccgc tatgtgattg cacaggcgaa
     5641 tgccgatttg gattcagatg gcaatctgat tggcgatttg gttacctgtc gtgaaaaagg
     5701 cgaaaccatt atggcaacgc ccgaccgcgt ccaatatatg gacgtggcaa ccggccaggt
     5761 ggtatccgtt gcggcatccc tgattccgtt cttggaacac gatgacgcga accgcgcatt
     5821 gatgggtgcc aacatgcaac gtcaggcagt gccttgcctg cgtcctgaaa aaccgatggt
     5881 cggtaccggt atcgagcgtt ccgttgccgt tgactctgcc actgcgatcg ttgcccgccg
     5941 aggcggtgtg gttgagtatg tcgatgccaa ccgcgtcgtc gttcgtgttc atgatgatga
     6001 agcgactgct ggcgaagtgg gtgtcgatat ttacaacttg gttaaattca cccgttccaa
     6061 ccaatctacc aacatcaacc aacgtccggc cgtcaaagcc ggcgatgtgt tgcaacgcgg
     6121 cgacttggtg gccgacggtg cgtctaccga cttgggcgaa ttggctttgg gtcaaaacat
     6181 gaccatcgcc ttcatgccat ggaacggtta caactatgaa gactcgattc tgatttcaga
     6241 aaaagtggct gcggatgatc gttacacttc gattcacatt gaggaattga atgtcgttgc
     6301 ccgcgatacc aagctgggtg cggaagacat tacccgcgat attccgaact tgtccgagcg
     6361 tatgcaaaac cgcttggacg aatccggtat cgtttacatc ggtgcagaag tagaagccgg
     6421 cgatgtgttg gtaggcaaag taacgcctaa aggcgaaacc cagctgacgc cggaagaaaa
     6481 actgttgcgc gccatcttcg gcgaaaaagc gtctgacgta aaagatactt cattgcgtat
     6541 gcctaccggt atgagcggta cggttatcga cgttcaagtc ttcactcgtg aaggtattca
     6601 acgcgacaaa cgcgcccaat ccattatcga ttccgagttg aaacgctacc gtttggattt
     6661 gaacgaccaa ttgcgtattt tcgacaacga cgcattcgac cgtatcgaac gtatgattgt
     6721 gggtcaaaaa gccaatggtg gtccgatgaa gctggctaaa ggcagcgaaa ttactaccga
     6781 atatttggcg ggtctgccga gcaggcacga ttggttcgat atccgtctga ccgatgaaga
     6841 tttggccaag cagttggaac tgattaaagt gagcctgcaa caaaaacgcg aagaagcgga
     6901 cgagttatac gaaatcaaga agaaaaaact gacccaaggc gacgaattgc aacccggcgt
     6961 acaaaaaatg gtgaaagttt ttatcgccat caaacgccgt ctgcaagccg gcgacaaaat
     7021 ggcgggccgc cacggtaaca aaggcgtggt atcgcgcatt ctgccagtgg aagacatgcc
     7081 ttacatggcg gacggccgtc cggtagacat cgtactgaac ccattgggcg taccttcccg
     7141 tatgaacatc ggtcagattt tggaagttca cttgggttgg gcagcaaaag gtatcggcga
     7201 gcgtatcgac cgtatgctga aagagcaacg caaagcaggc gagttgcgcg agttcttgaa
     7261 cagactctac aacggcagcg gtaagaaaga agatttggat gccctgactg atgaagaaat
     7321 catcgaactg gcctccaacc tgcgcaaagg tgcatctttc gcctctcctg tattcgacgg
     7381 tgcgaaagag tctgaaatcc gcgaaatgct gaacttggct tatccgagcg acgatcctga
     7441 ggttgaaaaa ctgggcttca acgacagtaa aacccaaatc acgctgtatg acggccgttc
     7501 aggcgaagca tttgaccgca aggttacagt aggtgtgatg cactatctga aactgcacca
     7561 cttggttgac gaaaaaatgc acgcccgttc taccggtccg tacagtctgg ttacccagca
     7621 gcctttgggc ggtaaagccc agttcggcgg ccaacgtttc ggcgagatgg aggtttgggc
     7681 attggaagca tacggcgcgg catacacgct gcaagagatg ttgactgtga agtctgacga
     7741 cgtgaacggt cgtaccaaaa tgtacgaaaa catcgtcaaa ggcgaacaca aaatcgatgc
     7801 cggtatgccc gagtccttca acgtattggt caaagagatt cgctcactgg gcttggatat
     7861 cgatttggaa cgctactgat acgggtttca gacggcataa ggggagctgt tctgcaggta
     7921 tgcggggcag ccgacaatgt ttaaaaacga aatgccgtct gaaaacactg tacctctatc
     7981 catatcgaaa atccgccatg cggtaaaaat acttccttca aggagcaaaa atgaatttgt
     8041 tgaacttatt taatccgttg caaactgccg gcatggaaga agagtttgat gccatcaaaa
     8101 tcggtattgc ctctcccgaa accatccgct catggtctta tggcgaagtc aaaaaacctg
     8161 aaaccatcaa ctaccgtacg ttcaaacctg agcgtgacgg tttgttctgt gccaaaatct
     8221 ttggcccggt caaagactac gaatgtttgt gcggaaaata caaacgcttg aaatttaaag
     8281 gtgtaacttg tgaaaaatgc ggcgtggaag taaccctgtc caaagtgcgc cgcgaacgca
     8341 tgggccatat cgaattggct gcgcccgtcg cacatatttg gttcttaaaa tccctgcctt
     8401 cccgcttggg tatggtgtta gacatgactt tgcgcgatat cgaacgcgta ttgtactttg
     8461 aagcatttgt ggtaaccgat cccggtatga ctccgctgca acgccgccaa ttgctgactg
     8521 aagacgatta ctacaacaag ctggacgaat acggcgacga tttcgatgcc aaaatgggtg
     8581 cggaaggtat ccgcgaattg ctgcgtaccc tgaatgtagc gggcgaaatc gaaatcctgc
     8641 gccaagagtt ggaatcgacc ggttccgaca ccaaaatcaa aaaaatcgcc aaacgcttga
     8701 aagtattgga agctttccat cgttccggta tgaaactgga atggatgatt atggatgtgc
     8761 tgccggtatt gccgcctgat ttgcgtccgt tggttccatt ggatggtggt cgttttgcca
     8821 cttccgattt gaacgatttg taccgccgcg ttattaaccg taacaaccgt ctgaaacgtc
     8881 tgttggaact gcatgctcct gacatcatcg tccgcaacga aaaacgtatg ttgcaagaag
     8941 cagttgactc gctgttggat aacggccgtc gcggtaaagc catgaccggc gccaacaaac
     9001 gcccgctgaa atcattggca gacatgatta aaggtaaggg tggccgcttc cgtcaaaacc
     9061 tgctgggcaa acgtgtggac tactccggcc gttccgtgat taccgtaggc ccgtacctgc
     9121 gtctgcacca atgcggtttg ccgaaaaaaa tggctttgga actgttcaaa ccgttcattt
     9181 tccacaaatt ggaaaaacaa ggtttggcct ctaccgttaa agcagcgaaa aaattggtag
     9241 agcaagaagt accggaagta tgggacatct tggaagaagt catccgcgaa catccgatta
     9301 tgctgaaccg tgcgccgacc ctgcaccgtt tgggtattca agcgttcgaa cctatcttga
     9361 ttgaaggtaa agcgattcag ttgcacccat tggtgtgtgc tgcgttcaac gccgactttg
     9421 acggcgacca aatggcggta cacgttccat tgagcttgga agcacaaatg gaagcacgca
     9481 cgctgatgct ggcttcaaac aacgtattgt ctcctgccaa cggcgaaccg attatcgtac
     9541 cttcccaaga catcgtattg ggcctgtact acatgacccg cgaccgtatc aatgccaaag
     9601 gcgagggcag cctgtttgcc gatgtgaaag aagtgcatcg cgcataccat accaaacagg
     9661 ttgaactggg tacgaaaatt accgtacgtc tgcgcgaatg ggtgaaaaac gaagcaggtg
     9721 agtttgagcc tgtcgttaac cgttacgaaa caaccgtcgg ccgtgcattg ttgagcgaaa
     9781 tcctgccgaa aggcctgccg tttgaatatg tcaacaaagc gttgaagaaa aaagaaattt
     9841 ctaaactgat taacgcatcg ttccgcctgt gcggcttgcg cgatacggtt atctttgctg
     9901 accacctgat gtacaccggt ttcggatttg cggcaaaagg cggtatttcc attgccgttg
     9961 acgatatgga aattccaaaa gaaaaagcgg ccttgctggc tgaagccaat gccgaggtta
    10021 aagaaatcga agaccaatac cgtcaaggtt tggttaccaa cggcgaacgc tacaacaagg
    10081 tggtcgatat ttggggtcgt gccggcgata agattgctaa agcgatgatg gacaacttgt
    10141 ccaaacaaaa agttatcgac cgtgacggca acgaagtcga tcaagagtca ttcaactcca
    10201 tttatatgat ggcggactcc ggtgcccgtg gttctgcagc tcagattaaa cagttgtccg
    10261 gtatgcgtgg cttgatggca aaacctgacg gctcgattat tgaaacgccg attacctcaa
    10321 acttccgtga aggtctgacc gtattgcaat actttattgc gacccacggt gcgcgtaagg
    10381 gtttggcgga taccgcattg aaaaccgcga actccggtta cctgactcgt cgtctggtag
    10441 acgtaactca agatttggtc gttgttgaag acgattgcgg tacttcagac ggctttgtca
    10501 tgaaggcagt ggtacaaggc ggtgatgtga ttgaagcatt gcgcgatcgt attttgggtc
    10561 gtgttaccgc gtctgacgtt gtcgatccgt caagtggcga aaccttggtt gaagccggta
    10621 cgttgctgac tgaaaaactg gtggatatga tcgaccaatc cggtgtcgat gaagtcaaag
    10681 tccgtacgcc gattacttgt aaaacccgtc acggcctgtg tgcacactgt tacggtcgtg
    10741 acttggcacg cggcaaactg gttaacgccg gtgaggcagt cggtgtgatt gctgcacaat
    10801 ccattggcga accgggtacc cagttgacca tgcgtacgtt ccacatcggt ggtgcggcat
    10861 cccgtgcggc agcagccagc caagtggaag ccaaatccaa cggtacggca cgattcagca
    10921 gccagatgcg ctacgttgcc aacaacaaag gcgagttggt tgtcatcggc cgttcttgtg
    10981 aagtcgtgat tcacgacgat atcggccgtg aacgcgaacg ccacaaagta ccttacggtg
    11041 ccatcctgct ggtacaagac ggtatggcca ttaaagccgg tcaaaccttg gcaacctggg
    11101 atccgcatac ccgtccgatg attaccgaac acgcaggtat ggtgaaattc gaaaacgtgg
    11161 aagagggcgt taccgttgcc aaacaaaccg atgatgtaac cggtttgtcc actttggtgg
    11221 tgattgacgg taaacgtcgt tcctctagtg cttccaaact gctgcgtccg actgtgaaac
    11281 tcttggacga aaacggcgtg gaaatctgta ttcccggtac gtccactccg gtttcgatgg
    11341 cattccccgt tggtgcggtg attaccgtac gcgaaggtca ggaaatcggt aaaggcgacg
    11401 tattggcgcg tatcccgcaa gcctcttcca aaacccgcga cattaccggt ggtctgccgc
    11461 gcgttgccga attgtttgaa gcacgcgtgc cgaaagatgc cggtatgttg gcggaaatta
    11521 ccggtaccgt ttccttcggc aaagagacca aaggcaagca acgtctgatt gttactgacg
    11581 tggacggtgt agcatacgag accttgattt ccaaagagaa acaaattctg gtacacgacg
    11641 gtcaagtggt aaaccgcggt gaaaccatcg tggacggagc tgttgatccg catgatattc
    11701 tgcgtttgca aggtatcgaa gcactggcac gctacattgt ccaagaggtg caagaggttt
    11761 accgtctgca aggtgtgaag atttctgata aacacatcga agtcatcatc cgtcaaatgt
    11821 tgcgccgtgt gaacattgcg gatgccggcg aaaccgggtt cattaccgga gagcaggtcg
    11881 aacgcggcga tgtgatggcg gccaatgaaa aagctttgga agaaggcaaa gagccggcac
    11941 gttacgaaaa cgtattgctg ggtattacca aagcctccct gtccaccgac agtttcattt
    12001 ctgccgcatc gttccaagaa acgacccgtg ttctgaccga agccgcgatt atgggcaaac
    12061 aagacgaact gcgcggttta aaagagaacg tcatcgtcgg ccgcctgatt cctgccggta
    12121 ccggtttgac ttatcaccgc agccgtcatc agcaatggca aggggtagag caggagactg
    12181 ctgaaaccca agtaacggat gaataatctt cggcgcgttt attcaataca aaaccgcaag
    12241 ctttaagctt gcggtttttc tttgtccgat taaggcaaaa acaagcgttt tcgtcatttt
    12301 gaggcgtgtg gattattcct taggtatttt cgggccggag accaacgagg tggcgggtgt
    12361 cgtcggtacg tccggagacc aaaataactt tgccaaggat gttggtttcg gcggtcaaaa
    12421 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    12481 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    12541 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    12601 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    12661 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    12721 tgaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    12781 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    12841 cccgcctttt attcgcgcag cagcaaggaa tggtttgtca gtatagaaaa aacgtattga
    12901 cagtattttc tccagccgtc cgactgattg tgagggatgt cggtaaatat ttatcggcaa
    12961 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    13021 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    13081 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    13141 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    13201 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    13261 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    13321 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    13381 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatacg gtgctaagcg
    13441 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagctga acaacggccg
    13501 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    13561 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    13621 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    13681 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    13741 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    13801 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    13861 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    13921 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    13981 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    14041 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    14101 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg
    14161 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    14221 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    14281 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    14341 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    14401 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    14461 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    14521 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    14581 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    14641 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    14701 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    14761 caaatactta ggcggcgacg agctgaccga agaagaagtc gtaggcgcgt tgcgtcaacg
    14821 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    14881 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    14941 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    15001 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    15061 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    15121 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    15181 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    15241 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgtatg gaattccccg agccggtaat
    15301 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    15361 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    15421 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    15481 attcggtgtg gaagcaaata tcggtgcgcc tcaagtggct taccgtgaaa ctatccgcaa
    15541 agccgttaaa gccgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca
    15601 cgttgtgatt gaaatggaac ctatggaacc gggtggtgaa ggttacgagt ttatcgatga
    15661 aattaaaggt ggtgtgattc ctcgcgaatt tattccgtct gtcgataaag gtatccgcga
    15721 tacgttgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt
    15781 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    15841 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    15901 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    15961 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    16021 acctctggca gaaatgttcg gttattcgac cgacctgcgt tctgcaaccc aaggccgcgc
    16081 tacttactct atggagttca agaaatattc tgaagctcct gcccacatag ctgctgctgt
    16141 aactgaagcc cgtaaaggct aatcaggcaa ataggccgtc cgaaaggctg aaatgatttt
    16201 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagc
//
LOCUS       DAWKQB010000033        16112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.033, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000033 DAWKQB010000000
VERSION     DAWKQB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.033"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(354..776)
                     /locus_tag="WJL14_000790"
     CDS             complement(354..776)
                     /locus_tag="WJL14_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ7655890.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(894..1565)
                     /locus_tag="WJL14_000791"
     CDS             complement(894..1565)
                     /locus_tag="WJL14_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ7655891.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1581..2207)
                     /locus_tag="WJL14_000792"
     CDS             complement(1581..2207)
                     /locus_tag="WJL14_000792"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ7655892.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNRETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2314..3120)
                     /locus_tag="WJL14_000793"
     CDS             complement(2314..3120)
                     /locus_tag="WJL14_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7655893.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKAEAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3379..3819)
                     /locus_tag="WJL14_000794"
     CDS             complement(3379..3819)
                     /locus_tag="WJL14_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ7655894.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3882..5288)
                     /locus_tag="WJL14_000795"
     CDS             complement(3882..5288)
                     /locus_tag="WJL14_000795"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ7655895.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5458..6573
                     /gene="lptF"
                     /locus_tag="WJL14_000796"
     CDS             5458..6573
                     /gene="lptF"
                     /locus_tag="WJL14_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ7655896.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6570..7640
                     /gene="lptG"
                     /locus_tag="WJL14_000797"
     CDS             6570..7640
                     /gene="lptG"
                     /locus_tag="WJL14_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ7655897.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7950..10535
                     /gene="acnB"
                     /locus_tag="WJL14_000798"
     CDS             7950..10535
                     /gene="acnB"
                     /locus_tag="WJL14_000798"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ7655898.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10677..11672
                     /locus_tag="WJL14_000799"
     CDS             10677..11672
                     /locus_tag="WJL14_000799"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ7655899.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11741..11842)
                     /locus_tag="WJL14_000800"
                     /pseudo
     CDS             complement(11741..11842)
                     /locus_tag="WJL14_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11896..12909)
                     /gene="ilvC"
                     /locus_tag="WJL14_000801"
     CDS             complement(11896..12909)
                     /gene="ilvC"
                     /locus_tag="WJL14_000801"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ7655900.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRTDLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12989..13282)
                     /locus_tag="WJL14_000802"
     CDS             complement(12989..13282)
                     /locus_tag="WJL14_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ7655901.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(13349..13840)
                     /gene="ilvN"
                     /locus_tag="WJL14_000803"
     CDS             complement(13349..13840)
                     /gene="ilvN"
                     /locus_tag="WJL14_000803"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ7655902.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13851..15578)
                     /gene="ilvB"
                     /locus_tag="WJL14_000804"
     CDS             complement(13851..15578)
                     /gene="ilvB"
                     /locus_tag="WJL14_000804"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ7655903.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
BASE COUNT         3779 a         4370 c         4082 g         3881 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta taattcaata aattaatata
       61 tggcttaaaa taacgggatt ctcgcctccc gcccgcccgc agaagcaggc gggtatcatt
      121 ttaaaacgcg gcatttaaaa tttgaccgaa aattgttgac aatccggaat caagtctgca
      181 caataccccg acaagtccaa gtattataaa ggctgaataa agaggaaaca gcaggcagat
      241 atattcggga ggtgcagtcc gaatatatct gcttttttat gcgcctccgg attgcctgcc
      301 gcacctttcc cttcagacgg catcagccgt ttctccataa tgccgcccga tgcctattta
      361 tctgccccgg caatttcaaa actgtgggta atctttgccg ctttgcccaa cataatcgaa
      421 gccgaacagt atttttcggc agacatctga acggcgcgct caatggccga ttctttcaaa
      481 tcatgcccga atactttgaa atggatgtgg atttcggtaa acacgcgcgg cgcatcgtcc
      541 gcccgtttcg ccgtaaccgt cgcacggcag tcagtcactt tctgacgctg tttttcggca
      601 atcatcacca catcgatgct cgaacagccc gccacgccca acagcagcat ttccaaaggg
      661 ctgggcccgc gcttggccgc accttctgcc gccgaaccct ccataacgac gctgtgcccg
      721 ccttccgtcg tgccgacaaa gcacattccg tctatccatt ttgatgtaac ctgcatggtg
      781 tcattcctga aaatagcgtt aaaaccgctt tgcatatggc gttattgtaa acaatttcaa
      841 gcggcttatg cagaaatatg gacaaaacgg caaaaaaaca cttgaaaacc gatttacggt
      901 ttggcggact ggccgttgat ctgcaccgat ttgagtttca gcgtataggt tttgccgtcg
      961 tcggtatagc cgatttgtgc cggaatattg ttcagggacg gtgcgaagaa atacattacc
     1021 gtatcgtcgc cgcgccgcac ccgatatttg acgacttcgg tttccacgcc gcctatgctg
     1081 tattttcctg tacccgcctt attcaaaccg ccgacggaat aaagtttttt gccgttggtg
     1141 attttcagcc ccgaggggag tttcgcgtca tttgccgcca actgccaggc aagcgtgaac
     1201 aaatccatag ccttggggct ttgctcggtt ttgctctcgc ccgctttgcc gtaggttacg
     1261 ctgccgtcgg cgaatttggc ttccgcatac agtttgcccc tgcgtatgtc tctatagtag
     1321 gtagggtgca gggtattgcc gacaaccgta ccgccggact cgaaacggat attgtatagc
     1381 ggcactttaa tcgtcgaaac gattttgtaa gcattgccgc tgcgttcaaa tgtcatcgtg
     1441 gcgggaatgc cgtagctgcc ggaatagtgc agcacggcgg attggggcag ccctgccgca
     1501 tacgcgcacg gcagggcggc ggacaaaatg gcggcggaaa atatattttt aaaagtcttc
     1561 atcatttgct cccgcccggt ttacgccgtc aggaaacggg cggcatcggc gttttccgaa
     1621 tttctgacgc ggtttccctc gataatcagg cggccggcgg caaaatcggc aacggctttc
     1681 ggataaagtt tatgctcgac agccaaaacc cgtgcggcaa tatcgtctgc cgtatcgccg
     1741 tcaagtatcg gcacaatccc ttgcgataca atcgggccgc aatccagttc ggcagtaaca
     1801 aagtgaatcg tacagcctgc aacgcggcaa cccgcttcca aagcgcgttc gtgcgtatga
     1861 agtccggtaa acgagggcag gatggacggg tgaatgttca tcagcctgcc ttcgtaacgg
     1921 gcgcaaaact cgggggtcag aatccgcata aaacctgcca aaaccaccaa gtcgggttga
     1981 tatgcgtcga ttttttccat catggccgta tcgaaggcaa gtcgtgattc aaagttttta
     2041 tgattcaggc tatcggtcgg gatgccgcgt tcggccgccc attgcaaacc ggcagccgtt
     2101 tctctgttgc tcaacacggc ggcaatgcgg acgttgggaa tggcggcatt gacgattgcc
     2161 tgcatattgc tgccgcgtcc ggaaatcagg atgacgatgt ttttcataat ggtacgcttt
     2221 tgaaagggat gccatctgaa ccgctgtttg gtggtttcag acggcatttg ccgtaaaaat
     2281 gcccgaaaac ctgtttcggg catggattcg gacttaattt acttttttga tgtcgacttg
     2341 agccggctgc ttggcgggcg cgttttcggg tgcgccgatt ttgaccagtt tcacatcaaa
     2401 taccaaagtg gcgttcgggc cgattttgtc gcccgcaccc tgttcgcggt aggcaaggtt
     2461 ggacgggatg tagaacgtgg cttcgccgcc ttctttcaga agctgtacgc cttcggtcca
     2521 acccggaatc acttggctca aagggaaggt gaccgggccg ccgttggctt tgctgctgtc
     2581 gaataccgta ccgtcaatca ggcggccttc gtattccacg gtaacgatgt cgtctttggt
     2641 cggctgtttg ccttcgccct gtttggtgat tttgtattgc aggccggaag cagtggtctt
     2701 cacgccgtct ttggcggcat tttctttcag aaaggcttcg cctttttctt tattggcctt
     2761 cgcttccgcc ttgtgttttt ctacggcttt agcctgttgt tcctgaagga atttcatcat
     2821 gacttcctga gcctgctctt cagtcatttt gatttctttg ccgtcataca ctgcctgcat
     2881 ggcttcggta aagactttca aatcgatttc cgcaccctgt tccttcattt gcttcaggga
     2941 gcgtccgatg tccacgccca tcgcatagct tgcctgctgc atcgtgctgc cgatcgaaga
     3001 ggtgtcgccc tgcgcggcag aagcggcggc aggttcggat gcggcttctt ttttgccaca
     3061 ggcggaaagt gccaaagcgg cggaaagggt cagtgcgctg attttgaaaa tggtgttcat
     3121 gatggatctt cgctgtcgat aaggtcggaa aaacgggatt atagccgagt ttgaatgttt
     3181 caacacacag gatgacacat aaagcgtcaa tcgtgtgttg ccctgttttg gaagggattg
     3241 aaccttccaa aataagtttt gattctaccg ccccgaggga cagatgtcca agtggcgggg
     3301 ttcaaccgat aaggaaattt taatcaaata gaatcaagcc tgtttaaatt ttgtaaatgc
     3361 ggcatttcag acggcatttt atgccttgcc ctccatgccg tgatgttcga tggcaaaacc
     3421 gcttcggcgg taggcggtaa agcgttcgcg cgcgtcggcg agatcttcca agctgttgcc
     3481 gacgatttcc aaaacgcgcg cgggcaagac cggagcggtg ttccaaaaac cgtcggacag
     3541 gttcaaaaca gtcatgcctt cgggaattcg gggcggattg ccgccgcaag caagcaggac
     3601 ggatgtttca gacggcatgg cttcttccat ttcccaaatt tcgtgcggaa tgaaactctc
     3661 ggcttcgtat tgccaaagca ttttgtccaa ttcctgaagc cgcccgaacg aatcggacca
     3721 caccagtatc ctgccgccgt cccgaatcgc acgggcaatc aggcggcagg tgaaaatcgg
     3781 aacttgggca acgtgcgtgt aaaaggtggc tttcggcata ttgtttgagc atttggcagg
     3841 acgatgccgt ctgaaaggct tcagacggca ttgtggcaaa attaaagatt ccgcagatag
     3901 ttcagcagca agggaacggg acggccagtc gcaccttttt ccgctccgga tttccacgcc
     3961 gtacccgcga tgtcaaggtg cgcccacggg tagccttcgg tgaagtaaga caggaatgtt
     4021 gcggcggtaa tcgtgcccgc gccgggcgtg ccgatgtttg gaatgtcggc aaagttggat
     4081 ttgagttggt ctttgtaggt ctcaaagagc ggcagttgcc atgctttgtc gtccacgttg
     4141 taggaagcgg caagcaggct gtcgatcaaa tcctgattgt tgcccatcac gccgctgaca
     4201 tcgtgcccca aggcaacaat acacgcgccg gtcagggtgg cgacgtcgat gacggctttg
     4261 ggtttgaact gctcggcgta agtgagcgcg tcgcacaaaa tcagacggcc ttcggcatcg
     4321 gtgttcaaca cttcgatggt cagacctttc atacttttca cgacatcgcc cggtttgttt
     4381 gccgcgccgg aaggcatatt ttcacaagtg gcgacgacgg caatcaggtt aatcggcagt
     4441 tgcagtttga cggcggcgca gaaggtgctg atgacggttg ccgcgccgca catatcaaac
     4501 ttcatttcgt ccatgttcag gccgggcttg agggagatgc cgccggtgtc gaaggtaatg
     4561 cctttgccga ccaataccac aggcgcggct tctttgtcgg ctgcaccgaa atagctcagt
     4621 tcgaccaaat agggggcttc cgcgctgcct ttggcgaccg accaaaacga acccatgttt
     4681 tctttgatgt agtctttttc gatgattttg gcgtgcgcgc ccagtttttc ggcttcggct
     4741 ttggcggtgc gcgctaaaaa ttcgggcgtg cattcgttgg gcgcggcgtt gcccaagtcg
     4801 cggcagaggc tttgtccgta aacttgcgct tcggcgacgc gcaaggcttc tttaacgacg
     4861 gcttcgtgtg cgctgtggaa cacggcggtt tcaaatttgg cgggcttggc ttcttttttg
     4921 tagcggtcga aacggtaggc ggcgttgccg aacgcaatcg caaacgcttc ggctacggcg
     4981 gcggcttgcg cttcatcgaa ggcatggatg tccacattga ccgtttcctg attttgcgcc
     5041 catttggcgg cttcggcggc ggccttgttc aatgcggcgc ggccggtgct tttcagacag
     5101 catacggcaa cagcctgcaa accgttgcct gtcgggattt ttgtgtcggc aaaattttga
     5161 ccttcttcaa gcgaagacaa aagggcaagg gcggtcggat ggtttaattg cgcctcttcg
     5221 gtgcagacaa ataactgtgc gcctgcctgc tgttcctgca agatttcggt ttttgtgcta
     5281 aattccacgt ttattctcct gattgagacg gttgtcggta gttttcggac ggcctttcgc
     5341 tcaaaagacc gtctgaagac ggctggcacg attgtacccc atttgaagca ccgtctgaaa
     5401 ccttgcgcgg acaatccgcc tgcgccgaac cgcttaccgc cccctgaccg cgattctatg
     5461 atttatcaaa gaaacctcat caaagaactc tcttttaccg ccgtcggcat tttcgtcgtc
     5521 ctcttggcgg tattggtctc cacgcaggca atcaacctgc tcggccgtgc cgccgacggg
     5581 cgtgtcgcca tcgatgccgt gttggcattg gtcggcttct gggtcatcgg tatgacgccg
     5641 cttttgctgg tgttgaccgc atttatcagt acgttgaccg tgttgacccg ctactggcgc
     5701 gacagcgaaa tgtcggtctg gctatcctgc ggattggcat tgaaacaatg gatacgcccg
     5761 gtgatgcagt ttgccgtgcc gtttgccgtt ttggttgccg tcatgcagct ttgggtgata
     5821 ccgtgggcag agctacgcag ccgcgaatac gctgaaatcc tgaagcagaa gcaggaattg
     5881 tctttggtgg aggcaggcgg gttcaacagt ttgggcaagc gcaacggcag ggtttatttt
     5941 gtcgaaacct tcgataccga atccggcatc atgaaaaacc tgttcctgcg cgaacaggac
     6001 aaaaacggcg gcgacaacat catcttcgcc aaagaaggta acttctcgct gaacgacaac
     6061 aaacgcacgc tcgaattgcg ccacggctac cgttacagcg gcacgcccgg acgcgccgac
     6121 tacaatcagg tttccttcca aaaactcaac ctgattatca gcaccacgcc aaaactcatc
     6181 gaccccgttt cccaccgccg taccattccg accgcccaac tgattggcag cagcaacccg
     6241 caacatcagg cggaattgat gtggcgcatc tcgctgaccg tcagcgtcct cctactctgc
     6301 ctgcttgccg tgccgctttc ctatttcaac ccgcgcagcg gacataccta caatatcttg
     6361 attgccatcg gtttgttttt aatttaccaa aacgggctga ccctgctttt tgaagccgtg
     6421 gaagacggca aaatccattt ttggctcgga ctgctgccta tgcacatcat catgttcgtc
     6481 atcgcaatcg tacttctgcg cgtccgcagc atgcccagcc agcccttctg gcaggcggtt
     6541 ggcaaaagtc tgacattgaa aggcggaaaa tgaacctgat ttcacgttac atcatccgtc
     6601 aaatggcggt tatggcggtt tacgcgctcc ttgccttcct cgctttgtac agcttttttg
     6661 aaatcctgta cgaaaccggc aacctcggca aaggcagtta cggcatatgg gaaatgctgg
     6721 gctacaccgc cctcaaaatg cccgcccgcg cctacgaact gatgcccctc gccgtcctta
     6781 tcggcggact ggtctctctc agccagcttg ccgccggcag cgaactgacc gtcatcaaag
     6841 ccagcggcat gagcaccaaa aagctgctgt tgattctgtc gcagttcggt tttatttttg
     6901 ctattgccac cgtcgcgctc ggcgaatggg ttgcgcccac actgagccaa aaagccgaaa
     6961 acatcaaagc cgcggccatc aacggcaaaa tcagtaccgg caataccggc ctttggctga
     7021 aagaaaaaaa cagcattatc aatgtgcgcg aaatgttgcc cgaccatacc ctgctgggca
     7081 ttaaaatctg ggcccgcaac gataaaaacg aactggcaga ggcagtggaa gccgattccg
     7141 ccgttttgaa cagcgacggc agttggcagt tgaaaaacat ccgccgcagc acgcttggcg
     7201 aagacaaagt cgaggtctct attgcggctg aagaaaactg gccgatttcc gtcaaacgca
     7261 acctgatgga cgtattgctc gtcaaacccg accaaatgtc cgtcggcgaa ctgaccacct
     7321 acatccgcca cctccaaaac aacagccaaa acacccgaat ctacgccatc gcatggtggc
     7381 gcaaattggt ttaccccgcc gcagcctggg tgatggcgct cgtcgccttt gcctttaccc
     7441 cgcaaaccac ccgccacggc aatatgggct taaaactctt cggcggcatc tgtctcggat
     7501 tgctgttcca ccttgccgga cggctcttcg ggtttaccag ccaactctac ggcatcccgc
     7561 ccttcctcgc cggcgcacta cctaccatag ccttcgcctt gctcgccgtt tggctgatac
     7621 gcaaacagga aaaacgctaa accaatgccg tctgaccccc ttcggacggc attggttttt
     7681 attatcatat tcctgtagac agatagccgt tccctattat agtggattaa caaaaaccag
     7741 tacggcgttg cctcgccttg ccgtactatt gtactgtctg cggcttcgtc gccttgtcct
     7801 gatttttgtt aatccactat acattacctg tcataacagt tccatttttg ttaaaactag
     7861 tctatgatag cggtacaaat attgtttaca atatttaacg caaatcattt gcaacccgac
     7921 aaaagaaaaa cagaaaaagg aacaaagaga tgttagaagc ctatcgtaaa gccgccgccg
     7981 agcgcgccgc cctcggcatt cccgccctcc ctttgaacgc gcagcaaacc gccgatttgg
     8041 ttgagctgct gaaaagcccg cccgcaggcg aaggcgagtt cttggtcgaa ctgcttgccc
     8101 accgtgttcc gcccggtgtg gacgatgccg ccaaagtcaa agcctcattc ctggctgccg
     8161 ttgccgaagg cagcgcgtcc agcccgctga tctcccccga atatgcgacc gaactcttag
     8221 gtacgatgct cggcggttac aatatccacg ccttaatcga actcttggac gacgacaaac
     8281 tcgcgcccat tgccgccaaa ggcttgaaac atacgcttct gatgttcgat tccttccacg
     8341 acgttcagga aaaagccgaa aaaggcaaca aatacgcgca agaagttttg caatcttggg
     8401 cagatgccga atggttcgcc tcacgcgcca aagttcccga aaaaatcacc gttaccgttt
     8461 tcaaagttga cggcgaaacc aatacagacg acctctcccc cgcgcccgac gcgtggagtc
     8521 gtcccgatat tccgctgcac gcgctggcca tgctgaaaaa cccgcgcgac ggcatcacgc
     8581 ccgacaaacc gggcgaagtc ggtccgatta aattgttgga agaactcaaa gccaaaggcc
     8641 atccggttgc ttacgtcggc gacgtggtcg gtactggttc ttcacgcaaa tccgcgacca
     8701 actccgtcat ttggcatacc ggcgaagaca ttccgttcgt gccgaacaaa cgcttcggcg
     8761 gcgtatgttt gggcggcaaa atcgcgccga ttttcttcaa tacccaagaa gattccggcg
     8821 cgctgccgat tgaagtcgat gtatctgctc taaaaatggg cgatgtcgtc gatatcctgc
     8881 cttatgaagg caaaatcgtg aaaaacggcg agactgttgc cgagtttgaa ttgaaatcac
     8941 aagtattgct ggacgaagtg caagccggcg gccgtatcaa cctgattatc ggccgaggtc
     9001 tgaccgccaa agcgcgcgaa gccctgaaac tgcctgcctc tactgcattc cgcctgccgc
     9061 aagcgcctgc cgaaagcaaa gccggtttca ccttggcgca aaaaatggtc ggccgcgcct
     9121 gcggtctgcc cgaaggacaa ggcgtgcgcc cgggtactta ctgcgaaccg cgtatgacga
     9181 cggtcggctc gcaagacacg accggcccga tgacccgcga cgagttgaaa gacttggctt
     9241 gtttgggctt ctccgccgat atggtgatgc agtctttctg ccacaccgcc gcctatccga
     9301 aacctgtcga tgtaaaaacc cataaagaac tgcccgcctt tatttccacc cgtggcggcg
     9361 tgtcactgcg tccgggcgac ggcgtcatcc actcgtggct caaccgcctg ctgctgcccg
     9421 ataccgtcgg caccggcggc gacagccata cccgtttccc catcggtatt tccttccccg
     9481 ccggctccgg cttggttgcc tttgccgccg caacgggcgt aatgccgctc gatatgcccg
     9541 agtctgtatt ggtacgcttc agcggcaagc tgcaaccggg cgtaaccctg cgcgatttgg
     9601 tgaacgccat cccgctgtac gcaattaaac aaggtttgct gaccgttgcc aaagccggta
     9661 agaaaaacat cttctctggc cgcatcctcg aaatcgaagg cctgcccgat ttgaaagtgg
     9721 aacaagcctt tgaattgacc gacgcatccg ccgaacgctc cgccgccggc tgtaccgtga
     9781 agctcaataa agagccgatt atcgagtaca tgaaatccaa cgtcgtgttg atgaaaaaca
     9841 tgattgccaa cggctatcaa gacccgcgca ctttggaacg ccgcatcaaa gccatggaaa
     9901 aatggctggc aaatcccgag ttgctcgaag cggataaaga tgccgaatac gccgccgtga
     9961 ttgaaatcaa catggacgac atcaaagagc cgattatcgc ctgcccgaac gacccggacg
    10021 acgtgtgctt catgtccgaa cgctccggca ccaaaatcga cgaagtattc atcggttcgt
    10081 gtatgaccaa catcggccac ttccgcgccg cctccaaact tttggaaggc aaggcagaca
    10141 cccccgtccg cctgtggatt gcgccgccga ccaaaatgga cgcgaaacaa ttgtccgacg
    10201 aaggacacta cggcgtactc ggacgtgccg gcgcgcgtat ggaaatgccg ggttgctcct
    10261 tatgtatggg taatcaggcg caagtacgcg aaggtgcgac cgttatgtcc acctccaccc
    10321 gcaacttccc gaaccgtttg ggtaaaaaca cctttgttta cctcggttcg gcggaattgg
    10381 cagcgatttg ctccaaactg ggtaaaatcc cgaccgttga agaatatcaa gccaatatcg
    10441 gcatcatcaa cgaacagggc gataaaatct accgctatat gaacttcaac gaaatcgaca
    10501 gctacaacga agtagccgag accgtgaacg tttaatcccc gtcatccgta tgaagtaagg
    10561 gattgaccgc aatgccgtct gaacaacctt cagacggcat tgcaacattc cgctaaccct
    10621 tctttccgca aacgctgcaa atacggcgtt cacgccccca cataaaggaa acgacagtga
    10681 acctgaaaaa ccgccatttt ctgaaacttt tagacttcac gccggaagaa atcaccgcct
    10741 acctcgacct tgccgccgaa ttgaaagccg ccaaaaaagc agggcgcgag attcagcgga
    10801 tgaaagggaa aaacatcgcc ctgatttttg aaaaaacctc tactcggacg cgctgcgcgt
    10861 ttgaagtcgc cgcgcgcgat caaggcgcgg gagtgactta tttagagccg tccgccagcc
    10921 aaatcgggca taaggaaagc atcaaagaca ccgcccgcgt gttgggcagg atgtacgatg
    10981 ccatcgaata tcgcggtttc ggtcaggaag ttgttgaaga attggcgaaa tacgcgggcg
    11041 tacccgtgtt caacgggctg accaacgagt tccatcccac acaaatgctt gccgacgcac
    11101 tgactatgcg cgaacacagc ggcaaacctt tgaaccaaac cgcgtttgcc tacgtcggcg
    11161 acgcgcgtta caacatgggc aattccctgc tgattttagg ggcaaaattg gggatggacg
    11221 tgcgtatcgg cgcaccgcaa agcctgtggc cgtctgaagg cattattgcc gccgcacacg
    11281 ccgccgccaa agaaaccggc gcaaaaatta ccctgaccga aaacgcgcat gaagccgtga
    11341 agaatgttga ttttattcat accgatgtgt gggtcagcat gggcgagccg aaagaagtct
    11401 ggcaggaacg catcgatttg ctgaaagatt accgcgttac gcccgaactg atggcggcat
    11461 cgggcaatcc gcaagtcaaa ttcatgcact gcctgcccgc cttccacaac cgcgaaacca
    11521 aagtcggcga atggatttac gaaaccttcg ggctgaacgg tgtggaagtt acagaagaag
    11581 tattcgaaag ccccgccagc atcgtgttcg atcaggcgga aaaccgtatg cacacgatta
    11641 aagcggtaat ggtcgcggct ctgggcgact gacagaactg tgcctgttta aattcatccg
    11701 caacacagat accgtctgaa cacgatgttc agacggtatc catatatagt ggattaaatt
    11761 taaaccagta cggcgttgcc tcgccttgcc gtactatttg tactgtctgc agcttcgtcg
    11821 ccttgtcctg atttttgtta atccactata aaaaaactgc ctacacgatg tgtaggcagt
    11881 cccgtttgaa aacaatcagt ttttgtcttg gtcaaccaat ttgttggcag taatccaagg
    11941 catcatggca cgcagttgtg cgccgacttt ttcaacttgg tggtcggcat tcaaacggcg
    12001 gcgggcagtc atagacgcgt agttgacgtt accctcttgg ataaacattt ttgcgtattc
    12061 gccggtttgg atgcgtttca gagcattgcg catggcttct ttgctggaag cattgaccac
    12121 ttcagggccg gtaacgtatt cgccgtactc cgcattgttg gaaatggagt agttcatatt
    12181 ggcaataccg ccttcgaaaa tcaggtcaac aatcagtttc atttcgtgca gacattcgaa
    12241 gtaagccatt tcaggcgcgt aaccggcttc ggtcagggtt tcaaaacctg ctttgatcaa
    12301 ctcgaccacg ccaccgcaca atacagcttg ttcgccgaac agatcggttt cggtttcttc
    12361 gcggaaagtg gtttcaatca caccgccttt ggtgccgccg ttggcagccg cataagacag
    12421 ggcgatgtct ttggccttgc cggaattgtc ttggtaaacg gcaatcagag aaggcacgcc
    12481 gccgccgcgt ttgtattcac tgcgtacggt atgacccgga cctttggggg caaccataat
    12541 cacgtccaag tcggtacgcg gaacgatttg gttgtagtgc acgttgaagc cgtgtgcaaa
    12601 tgccagcgtt gcgccttctt tcaaattggc tgtaacttcg gcgtgataga cggcaggcat
    12661 ggtttcgtca ggcagcagca gcataacgac atcggcttct ttggtcgctt cagcaacggt
    12721 tttgacgaca tgaccggctg cttcggcttt tttccaagaa gaaccttggc gcagaccaat
    12781 caccacgttt acacccgaat ctttcaggtt ggcggcatgg gcatgacctt gcgaaccgta
    12841 accgatgatg gcaacggttt tgcctttgat tagggacaga tcggcatctt tatcgtaata
    12901 gacttgcatt tgatttcctt taaggtaaat ggttgtcgaa gccttaaaat gttgagcggc
    12961 ttcggacggg ttaaacagag tgtgccgctt aatcggcaac ttcattcatc aatacgattt
    13021 ccaacgcttc ggttttgccg tcgacggact ggacgaaggc ttggaaatgc gcgctggcgt
    13081 tatgttcgtc aatagctgct tgagatttcc aattttccac gaaaacaaaa cggttcggtt
    13141 tgccgatttc ctgatggaga tcgtagctga tgttgccctc ttctgcacgg ctggctttga
    13201 ccagttcttt aaactgtgct gccagtgttt ctgtgtattc cggtttgacg gtaaccagtg
    13261 cgacaatttt aatgttcgac ataaatctct cctgccgttc gtttttcaga cgacattcaa
    13321 ataccgtgcc gtctgaaagg ttacggcgtt aaattttcaa aatacgctca ccgcgaccga
    13381 tgccggccgc gcctgtgcgt acggtttcca aaatttgggc gcgtccgacc gtttccaaaa
    13441 aggaatccag cttgtctgtc gagccggtaa tttcaatcgt atagctgcgg tcggttacgt
    13501 cgatgatgct gccccggtag atttcggtca agcgtaaaaa ttcgtcgcgg tctttgccgg
    13561 cggcacggac ttttaccaac atcagttcgc gttcgacaaa acggctttcg ttcaaatcga
    13621 ccactttaat cacttcaatc aatttattga gttgcttggt gatttgttcg atgacctgct
    13681 cgtcgccgtg ggtaacgatg gtcatacggg acagggtttt gtcttcggtc ggcgcaaccg
    13741 ccaaagaatc gatattgtaa tcgcgtgcag agaacaaacc gaccacgcgg ctcatcgcac
    13801 ctgattcgtt ttcaatcaga acagataaga tatgtcgcat ttgcctctcc ttacgccttt
    13861 tcgtccgcac gcatatgcgg cggaagtacc atttcgtcca aacctttgcc gttaccgacc
    13921 atgggcatca cattctgttt ctggtcggtc aggaagtcga taaacaccag cctgtctttt
    13981 tggttcaatg cttccaacaa cgcaccttcc acatcagact tcttgtccac gcggatgccg
    14041 atatgaccgt atgcctcggc aagtttgacg aaatcgggca aagaatcgaa ataagtttcc
    14101 gactctcggc cgccgtaata tatttcctgc cactggcgta ccataccgag ataaccgttg
    14161 ttcagcgtaa tgacgttaac cggaatccga tattggaaac aggtggacag ctcttggatg
    14221 ttcatctgga tcgagccgtc gccagtaata cagaatacgt cttgatccgg ggcggcaagt
    14281 tttgcaccaa tcgcataagg cagaccaacg cccatcgtac ccaaaccgcc ggagttgagc
    14341 cattggcgcg gacgttcgaa gggataatat tgagccgcaa acatttgatg ctgccctaca
    14401 tccgatgtga tgattgccga attgccggta atctcggcaa gcttctgaat cacatattgt
    14461 ggcttgataa tttcgctgcc gttgtcaaac cacaagcaat cgcgggaacg ccattcctct
    14521 atggttttcc accatttgcc caaagcatct tcagacggca cggactcttg tttttgccac
    14581 agcgcaacca tctcggacaa aacgtttttc acgtcgccga caatcggaat gtccgccttc
    14641 acgcgtttgg cgatgctgga aggatcgaca tcgatatgga taaccttctt cgccttctcg
    14701 aaaaatttgg acggtacgga aaccacacgg tcgtcaaaac gcgcacctac ggcaagaacg
    14761 acatccgcat tctgcatggc aaggtttgcc tcgtaagtac cgtgcatacc gagcataccg
    14821 aggaattggc ggtcgccgga aggataagcg cccaagccca tcagcgtacc cgtgcacgga
    14881 gcacccgtca ttcggacaaa tcgggtcagc tcttcagaag cattacccaa caccacgccg
    14941 ccgccaaaat agacgaccgg acgtttggca gatgccaaca tctgcacggc ctttttaatc
    15001 tgaccgatat gcccttgaac aaccggttga tacgaacgga taaaaatgtc ttcctgagga
    15061 tagctgaatt tcgccatcgc ctgcgtaaca tctttcggga catcaaccac cacgggcccc
    15121 ggtcggccgc ttgcggcaat ttggaacgcc tttttaatgg tttccgccaa ctcattgatg
    15181 tccgtaacca ggaaattgtg tttgacgcac ggacgggtaa tacccaccgt atcaacttct
    15241 tggaacgcat ccgtaccaat cagggaattg cctacctgcc cgctgatgac caccatcgga
    15301 atcgaatccg tataggcagt agcaataccg gtcagtgcat tggtaacgcc cgggccggat
    15361 gtaaccaatg ccacgcccac cttaccgctg acgcgcgcat acgcatctgc cgcgtgtact
    15421 gccgcctgct cgtgacgggt cagaatgtgc ttgaatttat tgagttggaa aagggcatcg
    15481 tagatttcga taaccgcacc gccgggataa ccgaaaacgt actcgacacc ttcggctttg
    15541 agactctgca ctatgatttg cgcgcctgat aactgcataa cgacctcttt tatacggttt
    15601 caaaccaata gggacaaacc gctttgccac agcacctgta atgcaattcc accaagcagc
    15661 gatttagggt acgcgcattg ggggaacacg gcaacagacg gattatccaa tcaattggaa
    15721 aggaacacag agtttgtgaa aaagagtaga aacgataacg caaaccgaca gttcaatcaa
    15781 gaaaaatctt tcatctttta atattttttg aaagcagaga aattattgat tgattttaaa
    15841 agaataaaat caggagtacc ttttttgaaa gatggaaatt gttgacagtt tgtgtaggag
    15901 gggcagatgt gaaaaaccct tcttcgatat caagaattgt aaaatttaca gggtttcatc
    15961 ccaataaaga ctcgggatat tgattgaact tgattttatt tttgatatat caaaaatatt
    16021 cccaaccata cttcctgaaa atggctcatt gcaccggact gtattggacg gcattgacag
    16081 aaccaagagg gctaacaacg acttaatata tt
//
LOCUS       DAWKQB010000034        15714 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.034, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000034 DAWKQB010000000
VERSION     DAWKQB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.034"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(52..807)
                     /locus_tag="WJL14_000805"
     CDS             complement(52..807)
                     /locus_tag="WJL14_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ7655904.1"
                     /translation="MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKDLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASVGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(987..2159)
                     /gene="nirK"
                     /locus_tag="WJL14_000806"
     CDS             complement(987..2159)
                     /gene="nirK"
                     /locus_tag="WJL14_000806"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ7655905.1"
                     /translation="MKRQALAAIIASMFALAACGGEQAAQAPAETPAASAEAASSATQ
                     AAAETPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQNTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAAPAASAPAASAPAASAPAKSDY"
     gene            2541..4796
                     /locus_tag="WJL14_000807"
     CDS             2541..4796
                     /locus_tag="WJL14_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ7655906.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRAKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPELNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            complement(5060..5764)
                     /locus_tag="WJL14_000808"
     CDS             complement(5060..5764)
                     /locus_tag="WJL14_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7655907.1"
                     /translation="MDFLERLKSLWPDNAKPADFYNKIDMSASGFSRVWKDGAIPTAD
                     YLIKIQEVTGCDLNWLLTGQGSPYMNKGQAVEVRTHTDGTATDTLGNPINLDEFVFIP
                     RYDVYAAAGHGYPAEDDKPLFCMAFRRYWIENYVTRQLDKLSVIAVKGDSMEGVLNHG
                     DNILVNHAETTPRDGLYVIRIDNHLFVKQIQKLPGKLLVKSANPVYEPFEIDLTDDNQ
                     NVAIIGRVEWYGRTVN"
     gene            5980..6243
                     /locus_tag="WJL14_000809"
     CDS             5980..6243
                     /locus_tag="WJL14_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7655908.1"
                     /translation="MQKNATPKNWHRADIVAALKKKGWSLRALSIEAGLSPNTLRSAL
                     AAPYLKGERIIAAAIGVEPEEIWPERYADRNLKPVFPKRVVNG"
     gene            6321..7211
                     /locus_tag="WJL14_000810"
     CDS             6321..7211
                     /locus_tag="WJL14_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7655909.1"
                     /translation="MLISAVELAGLQLPKLPNSRQGIEYHAKKNNWPFEEVVGQARGG
                     KLKKYLVSALPSEIQAAIQEKQAATLLAKVPMLPAEVKKPLRQNKKMRQLGLIPCEEG
                     LARLDDRQTETAHARCAIVAYVLPLHELAGMPIKKAVAFVAAEAAAGRLPEDVAKLIP
                     LANARNNGERSLSEPTLYRWVRAYRAASDSMSRLLALAPVKTREKTPLLAIDWLPYFL
                     MFYQRPNKPTMMAAAKKLAQWYLEQGKIEQMPSYDQIQTVMKRLPEHMKERGRRTGSA
                     YKALLPYIDRDWTALKPNDV"
     gene            7254..8366
                     /locus_tag="WJL14_000811"
     CDS             7254..8366
                     /locus_tag="WJL14_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7655910.1"
                     /translation="MGYLFTPEVTMIVDGCSGAVVGWSVALSETAVAVADALRHGMTH
                     FPPPLGYYSDNGSGETGDMLDKETTGILPRIGIEHFTGIPGNPQGRGKIERLWQTITI
                     PLAKQYATYQGKDADAETLRKVSNALASAQKVQKKGKELSKAQEAALSAAPTWAQFIA
                     DLEEAVRRYNFEHEHRSLPKNPEAGRHFTPMKYYEYRMETDGMRVKTDVLSPLELDFM
                     YRPEEERIPDRGAVSLHNNTYFHQELLDYSGQKVRVAYDIHDADHVIVKDMQGKVICK
                     AVFNGNKRAAFAETRMEQLAERRRKGQAKRLQDKMDLIEAQRQSAAPIIEQQPDFGEF
                     LKLETESGGLVEVENRPSESGRGKKKLRDFLWEDAG"
     gene            8408..9322
                     /locus_tag="WJL14_000812"
     CDS             8408..9322
                     /locus_tag="WJL14_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7655911.1"
                     /translation="MIEKLRQFLETSGMSQNKAADRMGVSRSALSGYLNGKYDGDIAG
                     MDKKAALFLEQEGDRAELKKLDIPYVETGTAKKMKGWLGLAAWLGQLGIVYGGAGLGK
                     TTVLKQYAATNPLALLIEPDTGYTAKVLLQEICHALDLSERGNIHELTERIINCLKRD
                     KKSRSPRDAHRILLIDEAEQLPTRALESLRRIHDKSGVAVALVGMPKLLLNLKGPNSE
                     FKQLFSRVSVKMELGEMLPETDLKQIAAAVLKTNDEAVLQKVVKTAKGNARKLSKLLL
                     IVDYLLQVNPDVDLDDDVIEHAETYLIH"
     gene            9398..9640
                     /locus_tag="WJL14_000813"
     CDS             9398..9640
                     /locus_tag="WJL14_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655912.1"
                     /translation="MENLKMRHLLAIPAIVIAVTACANRCTEPVPAWSLYQNETSQQR
                     LMREKQNAADFAVLQAEKAYERMSDKERMKGVVYEN"
     gene            9630..9929
                     /locus_tag="WJL14_000814"
     CDS             9630..9929
                     /locus_tag="WJL14_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655913.1"
                     /translation="MKTETKICKKCGEERPLFRFKKDDAYAGGYRPVCYECLNARAKE
                     LKAVRDSMAERKIISKGISINGGKTHMEFPEEPEFMKNKYWTVTKTDYRRPLKGL"
     gene            9932..10084
                     /locus_tag="WJL14_000815"
     CDS             9932..10084
                     /locus_tag="WJL14_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655914.1"
                     /translation="MSKRLARRKMPKHELKRVKAFALKQAVEKIRARWGEKAIRLAST
                     DNKQTD"
     gene            10100..10264
                     /locus_tag="WJL14_000816"
     CDS             10100..10264
                     /locus_tag="WJL14_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655915.1"
                     /translation="MMNEMNAVAALLVIAILLAAVMTVLVEILVEQRVKERLGKLLDK
                     ACRPDDGWQE"
     gene            10245..10460
                     /locus_tag="WJL14_000817"
     CDS             10245..10460
                     /locus_tag="WJL14_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655916.1"
                     /translation="MTGGRNDKEGNVMNMVLTIYFWLVGLVCFARSAKETYYAESLYG
                     LLTGLLGVMCSAFVVSVSVARVMGWLK"
     gene            10457..10771
                     /locus_tag="WJL14_000818"
     CDS             10457..10771
                     /locus_tag="WJL14_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655917.1"
                     /translation="MNQLKMTIAKPETEDFEDAWAFIRMLNLVTYDLNPLKTDTDGEY
                     EYLADEDKSDVLDAVVEKFNECSLEWMLSALQALMSPEMGIINQDSDTLELHPKLKGG
                     TE"
     gene            10890..11267
                     /locus_tag="WJL14_000819"
     CDS             10890..11267
                     /locus_tag="WJL14_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655918.1"
                     /translation="MRYLTLFRPAEKDLSFNRVSKLVGEIAPMIRDGKIVRNRKTYPA
                     PREAWIWAATRCLEARDAGKLTPPLTSHGYLLENITFWSPEKTAGTAVLPSPQPSHTR
                     EVASTKLRSGLGGLMEWSNGGKQ"
     gene            11251..11706
                     /locus_tag="WJL14_000820"
     CDS             11251..11706
                     /locus_tag="WJL14_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655919.1"
                     /translation="MEENNSWLKKAIAQGFMMLAALNLKGRPASADLTAVAELWLVIL
                     SGRSWQPEHDGIRIQAAFRAIAASSSEWPNPADLIKHLPPGEVRMVPRLEKKHCPTEY
                     GKEQAAELKKIVGRLKNAPCMNRDWIHGQRHRSVDECKRIYAERQKGNK"
     gene            11678..11872
                     /locus_tag="WJL14_000821"
     CDS             11678..11872
                     /locus_tag="WJL14_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655920.1"
                     /translation="MPKGRREINSQMSTFFDNQESDLINEFLTEYWEEFEKFSANNGF
                     DDDLLKQISMELEGHRNHYE"
     gene            11865..12476
                     /locus_tag="WJL14_000822"
     CDS             11865..12476
                     /locus_tag="WJL14_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3164 family protein"
                     /protein_id="HEZ7655921.1"
                     /translation="MSNLDMKQYKQDAKGNLVPLANIKEIDLLRDELVQEIAAKARAV
                     QDNLMAFKREAMDDIAAFVQLSADRYDVSVGGKKGNISLHSFDGAYRVNLAIQDTLVF
                     DEGLIAAKALIDECINEWTEGSRTELKTLINAAFQVDKEGNISTARVLGLRRLQITDE
                     KWQRAMDALSDSLQVHISKPFVRVYQRGEDGEYQLMNLDVAKV"
     gene            12482..13132
                     /locus_tag="WJL14_000823"
     CDS             12482..13132
                     /locus_tag="WJL14_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655922.1"
                     /translation="MNANIMIYLIEVNDGPVAKALRSFQQDKAAMRQAWLDWAREHLP
                     SDALMREWSDGQVAGFAFPSGIPDGWKKPNKNGICWPRQNNPILKTMPLNKRFKRPEE
                     YLEEVGITAPTMIFEKNSDGETWASWGIGNFFNPVQFVWAGLEEDAPKGVVTPDYAYE
                     LREGAKRIRNGCTMQPPEDFDWQNLLPGCRVIPRYEWDYLVGKWQETRNDAEEQEA"
     gene            13133..13333
                     /locus_tag="WJL14_000824"
     CDS             13133..13333
                     /locus_tag="WJL14_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655923.1"
                     /translation="MAKIIIEIEDLPEGTDINFKGDLPAADAKDKTGAQQTAVLISKM
                     IQAAQMMASPSVNIRPSETANP"
     gene            13349..13657
                     /locus_tag="WJL14_000825"
     CDS             13349..13657
                     /locus_tag="WJL14_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ7655924.1"
                     /translation="MPHLNLNRSQKVNKSELIQAIADEAELSKRAAAEFVNAFVSVVT
                     QELKDGNDVTLVGFGTFHAAQSAERQGRNPKTGEPLTIAARKTPKFRSGKALKDAVNG
                     "
     gene            13733..14167
                     /locus_tag="WJL14_000826"
     CDS             13733..14167
                     /locus_tag="WJL14_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HEZ7655925.1"
                     /translation="MMNTNEQYKRKGLIAKIKIAQSQLGMEDDVYRAMLARVCGKTSC
                     TQMNLAELQAVAAEMKRMGFKQTAPKGKGIRPHLTQDRAALLNKLEALLTVGDKSWQY
                     ADGMAKRMFGKDLVRFLTPEQLYKLVQALQIHINKAKKAAEQ"
     gene            14164..14610
                     /locus_tag="WJL14_000827"
     CDS             14164..14610
                     /locus_tag="WJL14_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655926.1"
                     /translation="MIRVDEEDFEAVRHLLPESVLALITIIGLTETVELVKNLGGTTY
                     PLRQGRTKGSESRLAYLEEIVGGAAMEKMVEALAPCDLFIPKCEQALLELRDRHIRRR
                     FDAQTGKGVPAYEAVNDLALAHALTDRHIWRILKKPDNECEQGGLF"
     gene            14658..15299
                     /locus_tag="WJL14_000828"
     CDS             14658..15299
                     /locus_tag="WJL14_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655927.1"
                     /translation="MKTFYVLMLAAMLAACGGQENKPAAGESQTDNRAQQYQNQQKQI
                     AARLADEEYFIGENNLEQIRLHANLKERAQKLLSLLAQADKESRVWVLPGDAAKIKEF
                     NAAFAAVAKSAEESFGGPFLADKAGLYQCTAAANAAYDYFFARQRKDALTENYRQQYT
                     DNIAACQGQIRTPPAAQATVYARKGIRLPLNNCLPVLAGDEEFDTYTCPMEVK"
     gene            15431..>15714
                     /locus_tag="WJL14_000829"
     CDS             15431..>15714
                     /locus_tag="WJL14_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7655928.1"
                     /translation="MSKIIVLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTV"
BASE COUNT         4047 a         3473 c         4512 g         3682 t
ORIGIN      
        1 aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct
       61 tgtgcaacgg aagcccaagt tgtgcaagac gtatttggat tgcaggctgg tgcggatgcc
      121 gtagcggagg aaggcggcat agttggacga gtcgctcgac ccgacagatg cgccgctgca
      181 aaacatttgg gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca
      241 ttcccaaatc agaccgtgca tatcataaac gccccagtag ttcgggcgac ctttgccgac
      301 atcgtgcagg tctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc
      361 gggttcgttt gagccgtttt tctgcgtggc ggaagcaagt ccagcaaatt cccattcgtc
      421 aatagtcggt aggcgtttgc cttgtgcggc gcaataggcg ttggcagcaa accaggaaac
      481 attggttacc ggttgtttta attcgcccgc cttcggcgca tagctgcggc tgccgttttt
      541 catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca
      601 ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag
      661 tttgaacggt ttgactttaa tcagaccggt atcttttttc agataaagcg ggcggtagct
      721 gccgccttcg atttgaacca tttcggcagc tgccgcttga gtgccggcga gtgccgcaca
      781 gagaaagagt aaccgggtaa acttcatatc atgcctcctg acaggcagtt aaaattgatt
      841 tttcagacgg cattttaaac aaaaagccgc gaatgccgtc tgaagaatca atcttccgaa
      901 aggaaagatt ggttgttaaa aaaccactgc cgtgcgtaat taagtacggc ggcagtggtt
      961 cgtttgccta aaaatgggtg ttcaatttag taatcgcttt ttgcaggagc agaggctgca
     1021 ggagcagaag ctgcgggagc ggaagcagca ggagctgcac cgttaccggc gtaagcggta
     1081 tcactcaatt tttgagtcat gatttcaggg ttttctgcac cttctacttt caattgaccc
     1141 agtgcgcctt tgttgaatgc gcggaagata gagtggtcaa ccaaagtgta gctgcctggg
     1201 atgtcgactt tgaattcgac aatggcagag ccgccggcag gaacgatggt attttgtacg
     1261 ttttcgttaa tcagtttgcc gccttcaaca taaactttgt cgaagatttc accgattacg
     1321 tggaaggaag acaccaagtt cggaccgccg ttaccaacgt acatacgtac agtttcgcct
     1381 gctttggctt tcagggcgtt gtcgccggcg atagcaccta cgtgaccgtt gaatacgacg
     1441 tattcaggct gttcggcaac ggctttgtcc atatcgaacg gttgcagacc ttgcgcgcct
     1501 tttttgcctt tggtgtagaa gtcgccttgg acgatgtaga actctttatc cactttcggc
     1561 aggccttctt taggctcgac caaaatcagg ccgtacatac cgttggcgat gtgcataccg
     1621 accggcgcga cggcgcagtg gtagatgtac agacccggtt gcagggcttt gaagctgaat
     1681 gtggaagtgc ggcccggggc ggtaaaggtc gcggctgcac cgccgccctg accggttgcg
     1741 gcgtggaagt cgacgttgtg cggaacggta gaagaaggat tgttggaaaa ttccacttca
     1801 accgtatcgc cttcgcgtac gcggatcatg cgacccggaa cgtcgccgtc aaatgtccag
     1861 tagcggtatt ccacaccgtc gtccattttc atggtttttt cgacggtttc cattttcacg
     1921 cgtactttgg cagggtagtc gcggtcgatt gcgggaggta cttcaggagc gtgggtggta
     1981 accgcatcga taacgggcag ttcgcctgcc ggagtttcgg cggcagcttg cgtagcggag
     2041 cttgcagctt cagcggaagc ggcaggggtt tcggcagggg cttgagcagc ttgttcgcca
     2101 ccgcaggcgg ctaaagcaaa catagaagcg ataattgcag ctaaggcttg gcgtttcatg
     2161 atgttttcct tttgtaagaa aagtaaattg tcctgaaagg attggttaag aactggtgtg
     2221 attatgcggt tgcttaccta tgtatatttt gatttaggtc aagtaattca ataaatatga
     2281 acttaaaatg acacttctaa tactttataa ctactaaaaa ttacaatgaa taggctgatt
     2341 tgctaggggc ttcggcagag caggcaaagg cagggcaatt tccgagtaaa gaataatgat
     2401 agttattatc atatatgtat ttgttttata tgtgagtttg tttgaacaaa tgtttatttg
     2461 cgcggtaaac cgtgctacaa tctttaacat tcatattctg tgaattttaa acaactgctt
     2521 tattaaaagg agctctcaaa atgggacagt acaagaagct gtggtacttg ctgtttgccg
     2581 ttctggcggt atgctttacc attcttggct atatgggcag cgaggtttat aagaaagccc
     2641 cgccttaccc cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata
     2701 ttttggcagg tcagtctgcg tggcagagta ccggcggtat ggaagtcggt tcgattctgg
     2761 gtcacggcgc atatcaggct ccggactgga cggccgactg gctacatcgt gagttgtccg
     2821 cttggttgga tttgaccgcg caacagactt acggcaaaaa attcgatgaa gtttcccctg
     2881 aagaacaagc cgttctgaaa acccgactgg ctgacgaata ccgcaaccaa agccgtgtga
     2941 aggaagacgg ttccgtagtc atcagtgata cgcgcgcgaa agccatcgaa agcatcctgc
     3001 cttattacca cggtgtttac ggcgacgatc ccgcgctgca aaccacccgc gaacactttg
     3061 caatgaaaaa caacacattg ccaagtcagg aagcgcgtga aaaactgttc gacttcttct
     3121 tctggacttc ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca
     3181 actggccgca cgagcctttg attaacaatg taccgactac tgaaaactac atgtggtcgt
     3241 ttaccagtgt cgtactgctc ttgatgggca tcggtttgtt gatgtggggt tattccttcc
     3301 tgaccaaaca cgaggaagtg gaagtgccgt ctgaagaccc gatttccaaa atccaactga
     3361 ccccttcgca aaaagcattg ggcaaatacg tcttcctgac ggtcgctctg tttgtggtac
     3421 aagtattgct gggcggcctg accgcgcact acaccgtcga aggtcagggt ttctacggca
     3481 tcgatgaggc gttgggcttt gaaatgtccg actggttccc ctatgccctg acccgtactt
     3541 ggcacatcca atccgccatc ttctggattg caaccggctt tttgacggcg ggtttgttcc
     3601 tcgcgccgat tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtgaacttcc
     3661 tctatatcgc cctcttcatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga
     3721 cgcacatcct tccccccgaa ttgaatttct ggttcggaca ccaaggttac gaatacctcg
     3781 atttgggccg tttctggcaa ctcctgctga tggtcggcct gttgttgtgg ctgttcctga
     3841 tgttgcgctg cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa
     3901 tctttgtcgc ttctatggtc ggtgtgggcg tattctacgc accgggtctg ttctatggcg
     3961 aaaaatcccc gattgccgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg
     4021 aaggcttctt tgaagtattt gccactgccg cctttgcctt tgtcttctac aatatgggct
     4081 ttgtccgccg cagtaccgcc actgcctcta ctctggccgc tgccgccatc tttatgttgg
     4141 gcggcgttcc gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg
     4201 caatcggcgc ctgcttctcc gctttggaag tcgtgccgct ggtgttgctg ggccgtgaag
     4261 catacgagca ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc
     4321 tgatgtgctt cgtggcagtc gccttttgga atatgatcgg tgccggtgta ttcggtttcc
     4381 tgattaaccc gccgatttcc ctgttctata tccaaggttt gaatacttct gctgttcatg
     4441 cgcacgccgc cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg
     4501 cccgttattt gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggc
     4561 tgctcaacgg cggcttggtc ggtatgattg ccatcagcct gttgccggtt ggcgtgattc
     4621 aggcatacgc ctccatcacg cacggtctgt ggtatgcccg cagcgaagag ttcctgcaaa
     4681 tggaaatcct cgataccttg cgttgggtac gtacggcagc tgacttggtc tttatcggcg
     4741 gtgcaatctg cgttgccatc caagctacta aaatcgtatt cggccgcgac aaataagttt
     4801 tgcggtatga aatgagcaac ttatggggta ggtattattt tgacaccttt agaaagaaag
     4861 caaaagaagg cgaaaatttt aaaggaaaca aaaatttgaa cggaaattta ggaaaaagcg
     4921 gctttgacac ctttaggtgc aaagtaggta gaaagtgata taactttgat accttttttg
     4981 cacggttttg atgaggtttt gggttgattt tggttttatt tgaattgaac ggcgtttgga
     5041 atatccaaac gccgtttttt tagttgacgg tacggccata ccattctacg cgtccgatga
     5101 tggctacatt ttggttgtcg tcggttaaat ctatttcgaa aggctcataa accggattgg
     5161 ctgatttgac gagcagcttg ccgggtagtt tttggatttg tttgacaaac aggtgattat
     5221 cgatgcggat aacgtacagg ccgtcgcgcg gggtggtttc ggcgtgattc actaggatgt
     5281 tgtcgccgtg gttgagtacg ccctccatcg aatcgccctt tacggcgatg acggacagtt
     5341 tgtcaagctg gcgggtaacg tagttttcta tccaataacg cctgaatgcc atgcagaata
     5401 agggcttgtc gtcttctgcg ggatagccgt gccctgcggc ggcgtagacg tcgtaacgcg
     5461 ggataaagac gaactcgtct aagttgatgg ggttgccgag ggtatcggta gccgtaccgt
     5521 cggtatgggt acggacttcg accgcttggc ctttattcat gtatggcgag ccttggccgg
     5581 taagaagcca gtttaaatcg caaccggtta cttcttgaat tttaatcaga tagtctgctg
     5641 ttggaatggc accgtctttc cagacacggc taaaaccgga ggcagacata tcaatcttat
     5701 tgtagaagtc ggcgggtttg gcattgtccg gccacaaaga tttcaaacgc tctaaaaagt
     5761 ccatgattgt ccttttgctt agaatttcgc atttaagcaa aaatacactt cttttgctta
     5821 aaattaattc gtcaaataat caaatagatg gctaatattt tgcttttttg cgttatattt
     5881 ctaagcaaaa gagttgcaat aacctttttt gcttagtatt attcactcac ttaatcaatt
     5941 aacgaagtaa atcacattac tgaatggaat aagtcaatta tgcaaaaaaa cgcaacgccg
     6001 aaaaactggc accgcgcgga tattgttgct gctttgaaaa agaaaggctg gtcgcttcga
     6061 gcactttcaa tagaagcggg gttgtcgccg aatacgctta gaagcgcact ggccgcccct
     6121 tatcttaagg gagaaaggat tattgccgct gcaatcggag tggaaccgga agagatttgg
     6181 cccgaacggt atgcagatcg gaatttaaaa cctgttttcc ccaaaagagt agttaatgga
     6241 taagtggttt tcgatttagt tccttaaatg ctaaaggtta tacgcattta agtaaaacac
     6301 aaaaagaaag aaatagcaaa atgttgattt cagcggttga gttggcgggc ttgcaacttc
     6361 caaagctgcc gaacagtaga caaggcatcg agtaccacgc caaaaagaat aattggccgt
     6421 ttgaagaggt tgtaggacag gctcgaggcg gcaagcttaa gaaatatttg gtttccgccc
     6481 tcccttcgga gattcaggcg gccatacaag aaaaacaggc ggcaacattg ttggccaagg
     6541 tgccgatgtt gccggcagaa gtaaaaaaac cgcttcggca aaataaaaag atgcggcagt
     6601 tgggtttgat accgtgcgag gaaggtttgg cgcggttgga cgacaggcag acggaaacgg
     6661 cccacgcgcg ttgtgcgatt gtcgcctatg tattgccgct gcatgaattg gcgggtatgc
     6721 cgattaagaa ggcggtggcg tttgtggcag ctgaagcggc agcaggcagg ttgccggagg
     6781 atgtggccaa gctgattccg ttggcgaatg cgcggaacaa cggcgaacgc agtttaagcg
     6841 aaccgacttt ataccgatgg gtgcgggctt accgtgcggc atcggacagt atgagccgcc
     6901 tcttggcact tgcgccggtg aaaacacggg aaaagacgcc gctgctggcg attgactggc
     6961 tgccttactt cttgatgttt tatcagcggc cgaacaagcc gacgatgatg gcggcggcga
     7021 agaaactggc gcaatggtat ctggaacagg gaaaaatcga gcagatgccg agctatgacc
     7081 agattcaaac agtgatgaaa cggttgccgg aacatatgaa ggagcgcggc aggcgcacgg
     7141 ggtcggctta taaggctttg ctgccttaca ttgatagaga ttggacggcg ttgaaaccga
     7201 acgatgtcta ggtgggcgac ggccacagct ttaaagcaaa aatccggcat ccgatgggct
     7261 atctgtttac gccggaagtg acaatgattg tggacggttg cagcggtgcg gtcgtgggtt
     7321 ggagtgtggc gctgtcggaa acggcggtag cggtggcgga tgctttgcgc cacgggatga
     7381 cgcatttccc gccacctttg ggttattact cggataacgg ctcgggtgaa acgggcgata
     7441 tgctggataa ggaaacgacg ggtattttgc cgcgtatcgg tatcgagcat tttacgggca
     7501 ttccgggcaa tccgcagggg cgcggcaaga ttgaacggct gtggcagacc attacgattc
     7561 ctttggccaa gcagtatgcg acttatcagg gcaaggatgc ggatgccgaa acattgagaa
     7621 aggtatcgaa tgcgctggcg agtgcgcaga aggtgcagaa aaagggcaag gaactgagca
     7681 aggcgcagga agcggcgctg tcggcggcac cgacttgggc gcagtttata gcggatttgg
     7741 aagaagcggt acgccgatac aactttgaac acgagcaccg cagcctgccg aaaaatccgg
     7801 aagcgggacg gcattttacg ccgatgaaat attacgaata ccggatggaa acggacggca
     7861 tgagggtgaa aacggatgta ttaagcccgc tggagttgga ttttatgtac cgtccggagg
     7921 aagaacgcat ccccgaccgc ggagcggtgt ctctgcacaa taatacttac ttccaccaag
     7981 aattgctgga ttacagcggt cagaaggtac gggtggctta cgacatccac gatgccgacc
     8041 atgtgattgt gaaggatatg cagggcaagg tgatttgcaa agcggtattc aacggcaaca
     8101 aacgggcggc atttgcggaa acgcggatgg aacaactggc cgaacgccgc cgcaaaggcc
     8161 aggccaaacg tctgcaagac aagatggatt taatcgaggc acaacgccaa tcggccgcgc
     8221 cgattatcga gcagcagcct gatttcggcg aatttttgaa gctggaaacg gaaagcggcg
     8281 ggttggtaga ggtggaaaac aggccgtctg aaagcggcag gggcaagaag aagttacgcg
     8341 attttttatg ggaagacgcc ggttagtaaa acggcacatc tttgacactt tttaaggaaa
     8401 acgaaaaatg attgagaaat taagacaatt tctggaaacc agcggcatga gccaaaacaa
     8461 ggcggccgac cgcatgggcg tatcgagaag tgcattgtcg ggctatttga acggcaaata
     8521 cgacggcgat attgcaggga tggataagaa agccgcgctg tttcttgaac aggaaggcga
     8581 ccgggcggaa ttgaaaaagc tggatattcc ctatgttgaa acgggcacgg ccaagaagat
     8641 gaaaggttgg ctgggtttgg cggcatggct ggggcagctc ggtattgtgt atggcggtgc
     8701 gggcttgggt aaaaccacgg tattgaaaca atatgcggcc actaacccgc tggctttgtt
     8761 gattgagccg gatacaggct atacggcaaa ggtgttgttg caggaaatct gccatgcact
     8821 ggatttaagc gagagaggca atatccatga gctgacggag cggattatca actgcctgaa
     8881 gcgtgataag aagagccgca gcccgcgtga tgcacaccgt attttgctga ttgacgaggc
     8941 ggaacagtta ccgacacgcg cactggagag cctgcggcgg atacatgata aatcgggcgt
     9001 ggcggtggcg ttggtgggta tgccaaagct gctgctgaat ctgaaggggc cgaacagtga
     9061 attcaagcag ttgttttcaa gggtatcggt caagatggaa ttgggcgaga tgctgccgga
     9121 aacggattta aaacagattg cggcggcagt gttgaagacg aacgatgagg cggtattgca
     9181 gaaggtggtg aaaacggcca aaggcaatgc gcggaaactg tcgaagctgc tgctgattgt
     9241 ggactatttg ttgcaggtta accctgatgt tgatttggat gatgatgtaa tcgaacacgc
     9301 ggaaacctat ttaatccact aaacctttga cagataaggc aataattttt ttaccttgtt
     9361 attttgttaa gttattgaaa ttaaaggaaa actaaaaatg gaaaatttga aaatgcggca
     9421 tctgctcgcc ataccggcga ttgtgattgc ggtaacggcg tgtgcgaacc gctgcaccga
     9481 gccggtgccg gcgtggagcc tgtatcagaa cgagaccagt cagcagcggc tgatgcggga
     9541 aaaacagaat gcggctgact ttgcggtatt gcaggcagaa aaagcctatg aacggatgag
     9601 cgataaagag cggatgaaag gggtggttta tgaaaactga gacgaaaatc tgcaaaaaat
     9661 gcggagaaga gaggccgttg ttccgtttca aaaaagacga tgcgtatgcg ggcgggtata
     9721 ggcctgtatg ttacgagtgt ttaaacgcca gagcaaagga actgaaggca gtccgagaca
     9781 gtatggcgga aagaaagatt atctctaaag gcatcagcat aaatggaggg aaaacgcata
     9841 tggaatttcc tgaagaacct gaatttatga aaaacaaata ctggaccgtt acaaaaacgg
     9901 attatagaag accattaaaa ggactttaac catgagtaaa cggttggcac gacggaagat
     9961 gccgaaacac gaattgaaac gggttaaagc gttcgccctg aaacaggcgg tcgagaagat
    10021 tcgggcacgg tggggcgaga aagcgatacg gcttgcctca acagacaaca aacagactga
    10081 ttaaacaagg aataagaaaa tgatgaatga aatgaatgcg gtagcggctt tgttggttat
    10141 tgccatattg ctggcggcgg taatgactgt gttggtcgag atattggtcg agcaacgggt
    10201 taaggaacgg ctggggaaac tgctggataa ggcgtgccga ccggatgacg ggtggcagga
    10261 atgataagga aggaaatgtg atgaatatgg tactgacaat ttatttttgg ttggtggggc
    10321 tggtctgttt tgcccgttcc gccaaagaga cttattacgc ggaatcttta tacggtctgc
    10381 tgactgggct gttgggggtg atgtgttcgg cttttgtggt gtcagtgtcg gtggcaaggg
    10441 tgatggggtg gttgaaatga atcaattgaa aatgacgatc gccaaacctg agacggaaga
    10501 ttttgaagat gcctgggcat ttatcagaat gcttaatttg gtgacttatg atttgaaccc
    10561 tctgaaaact gataccgacg gcgaatacga atatttggcg gatgaagaca aatctgatgt
    10621 attagatgcc gttgtcgaaa aatttaacga atgtagtctt gagtggatgt tgtcggcgct
    10681 gcaagcactg atgtcgccgg agatggggat tatcaaccaa gattcggata cgttggagtt
    10741 gcatcccaaa ctgaaaggcg gtacggaatg aaagtgcgct gccctacctg cggtgcggtg
    10801 atgagcttag atgtattaat cgcccatgat gatgcagcga agctctgatt gccctgacag
    10861 gcatttcaga cgaccttttt aaggcggtat tgcggtatct gacgctgttt cgccccgccg
    10921 aaaaggattt aagttttaac cgagtttcaa agctcgtcgg cgagattgcg ccgatgatac
    10981 gggacggaaa gattgtccgc aaccgcaaaa cttatccggc cccgcgcgag gcttggattt
    11041 gggcggcaac gcgatgcctt gaggcacggg acgcgggaaa actgacaccg ccactgacca
    11101 gccacggtta tttgttggaa aacattacgt tttggtcgcc tgaaaagacg gcgggaacgg
    11161 cggttttgcc ctctccccaa ccatctcaca cgagagaggt ggcaagcacc aaattgagga
    11221 gcgggttggg cggcttgatg gagtggtcaa atggaggaaa acaatagctg gctgaaaaaa
    11281 gcaatcgcgc agggtttcat gatgctcgcc gccctaaacc tcaaaggccg ccctgcctcg
    11341 gcggatttga cggcagtcgc cgaactttgg ttggtcatac taagcggccg gtcgtggcag
    11401 ccggagcatg acgggatcag gatacaggca gcctttaggg ctatcgcggc gtcctcgtca
    11461 gagtggccaa accctgccga ccttatcaag cacctgccac cgggcgaagt caggatggtg
    11521 ccgaggctgg aaaagaagca ctgcccgacg gaatacggca aagagcaggc cgccgaactg
    11581 aaaaagattg tcggcagatt gaaaaacgca ccctgcatga atagagattg gatacacggg
    11641 caacgccacc ggtcggtgga cgaatgtaaa aggatttatg ccgaaaggca gaagggaaat
    11701 aaatagccaa atgagtacgt tttttgacaa tcaggaatcc gacttaataa acgaatttct
    11761 gacggaatac tgggaagagt ttgaaaaatt ctccgccaac aacggatttg atgacgattt
    11821 attgaagcaa atatcaatgg aattggaagg acataggaac cattatgagt aacttagaca
    11881 tgaaacaata caaacaggat gccaaaggca atctcgtgcc gctggccaat attaaagaaa
    11941 tcgacctgct gcgcgacgag ctggtgcagg aaattgccgc caaagcccgc gcggtacagg
    12001 ataacttgat ggcgttcaaa cgcgaggcga tggacgatat cgcagcgttt gtacagttga
    12061 gtgccgaccg ctatgacgta tctgtcggcg gtaagaaagg caatatcagc ctgcacagct
    12121 ttgatggagc gtaccgcgtc aaccttgcca tacaggacac gttggtattt gacgaaggtt
    12181 tgattgccgc caaagccctg attgacgagt gtatcaacga atggacggaa ggcagtcgca
    12241 cggaattgaa aacactgatt aacgcggcct tccaagtgga caaagaaggc aatatcagca
    12301 ctgcccgcgt cctcggtctg cgccgcctgc aaatcacgga tgaaaaatgg caacgggcga
    12361 tggatgcgct ctccgacagt ttgcaggtgc atatcagcaa accgtttgtg cgggtatatc
    12421 agcgcggcga ggatggggag tatcagttaa tgaatttgga tgtggcgaag gtgtaggaaa
    12481 aatgaacgca aatataatga tttatttgat tgaagttaat gatgggccgg ttgcaaaagc
    12541 actgcgaagt tttcagcagg acaaggccgc aatgcggcag gcgtggcttg attgggcgcg
    12601 agaacacttg ccgtcagacg cgctaatgcg ggaatggagc gatgggcaag tggcagggtt
    12661 tgcgttccct tcaggcatac ccgatggatg gaaaaagccc aataagaacg gaatttgttg
    12721 gccacggcaa aacaacccaa tcctcaaaac catgccactt aacaaaagat ttaaacgccc
    12781 cgaagagtat ttggaagaag ttggcatcac cgcaccaaca atgatttttg aaaagaatag
    12841 cgacggagag acttgggcat cttggggtat cggtaatttc tttaatcctg ttcagttcgt
    12901 ttgggcggga ttggaggagg acgcgccaaa gggcgtggta actcctgatt atgcctacga
    12961 gcttagagaa ggggctaaga gaatcagaaa cggctgtacg atgcagccgc ccgaagattt
    13021 tgattggcag aatctgttac cgggctgccg tgtcattcct cggtatgagt gggattactt
    13081 ggttgggaaa tggcaggaaa cacggaatga tgccgaagaa caggaggctt aaatggcaaa
    13141 aatcattatt gaaatcgaag acctgcccga gggcaccgac attaatttta agggcgacct
    13201 tccggcggcg gatgcaaaag acaaaacagg tgcgcagcaa acggctgtgt taatcagcaa
    13261 gatgattcag gcggcgcaga tgatggcatc cccatccgta aacattaggc cgtctgagac
    13321 tgcaaatcca taacccgcgc ggcacggtct gccgcattta aatctaaata ggagtcaaaa
    13381 agtgaataaa tccgaattaa tccaagccat cgccgacgag gcggaattga gcaaacgcgc
    13441 tgcggcggag tttgtcaatg cgtttgtcag cgtggtaacg caagaactga aagacggaaa
    13501 cgacgttacg ctggtcggct tcggcacgtt ccacgccgcc caatccgccg agcgtcaggg
    13561 acgcaatccg aaaacgggcg aaccgctgac catcgcggcg cgcaaaacac ccaagttccg
    13621 ctcgggcaag gcgttaaaag atgcggtaaa cggttaaacg tctgaaaccc caaatccaag
    13681 cggatttttg aaaagtccgc ttcagtttga agttttagcg acgaggaaaa ccatgatgaa
    13741 cacaaacgaa cagtacaaac gcaaggggct gattgcgaaa atcaaaatcg cgcaaagcca
    13801 gcttgggatg gaagacgatg tttaccgtgc gatgctggcg cgtgtgtgcg gcaaaacatc
    13861 ctgcacgcag atgaatctag ccgaattgca ggcggtggcg gcggaaatga agcggatggg
    13921 ctttaagcag accgcaccga agggcaaagg tatccgcccg catttgacac aggaccgtgc
    13981 cgcgctgttg aacaagctgg aagcgctgtt gacggtgggc gataaaagct ggcagtacgc
    14041 cgacgggatg gccaaaagga tgttcggcaa ggatttggta cgatttttga cacccgagca
    14101 gctatacaaa ctggtgcagg cgctgcaaat ccatatcaac aaagcgaaaa aggcggcaga
    14161 gcaatgatca gggtggatga agaagatttt gaggcggtgc gccacctgct gcccgaaagt
    14221 gtgctggcac tgattacgat catcgggctg acagaaacgg tggagctggt gaaaaactta
    14281 ggcggcacga cctacccgtt gcggcaggga cgcaccaaag gcagcgaatc gcggctggct
    14341 tatttggagg aaatcgtcgg cggtgcggcg atggaaaaaa tggtggaagc actggccccc
    14401 tgcgatttgt ttattccgaa atgcgagcag gctttgctgg aattgcgtga ccgccatatc
    14461 cgccgccgct tcgatgcgca gacgggtaaa ggcgtaccgg cttatgaggc ggtgaacgat
    14521 ttggctttgg cgcacgcgct gaccgaccgc catatctggc ggattctgaa gaagccggat
    14581 aatgagtgtg agcaaggcgg gttgttttaa attataatgc cgtggggata acacggcatt
    14641 ttttatggga aatcaagatg aagacgtttt atgttctgat gttggccgca atgcttgcgg
    14701 cttgcggcgg acaagaaaac aagcctgctg ccggcgaaag tcaaaccgat aatcgggcgc
    14761 agcaatatca gaatcagcaa aaacagattg ccgcacgttt ggctgatgaa gaatatttca
    14821 tcggtgaaaa caatttggaa caaatccgtc tgcacgccaa tttgaaagag cgcgctcaaa
    14881 agctgttgtc tttattggcg caggccgaca aggaaagccg tgtgtgggtt ttgcccggcg
    14941 atgcagccaa aatcaaagaa ttcaatgcgg ctttcgcggc ggtggcaaaa tctgccgagg
    15001 aatctttcgg cggccctttt cttgcggata aggcggggct ttatcaatgt accgctgcgg
    15061 ccaatgcggc ttacgattat tttttcgccc ggcagagaaa ggatgcgctg accgaaaatt
    15121 accgtcaaca atacacggac aacatagctg cctgccaagg gcaaatccgc accccgccag
    15181 ccgcccaagc gacagtgtat gcgcgtaaag gtatcaggct gccgttgaat aattgtttgc
    15241 cggtattggc cggagatgaa gaatttgata cctacacctg cccgatggaa gtaaaatagg
    15301 ccgtctgaaa cccagcccga gagcaaaagc cctcgggctt tttgctgcct gtctgacatg
    15361 gggcaggcgc ggtgccaaat aagcggacag gataatcaag gctcgataac agactaagca
    15421 ggacgaatct atgagcaaga ttattgtgct gaccgcaggc cacagcaaca ccgacccggg
    15481 tgcggtcaac ggaagcgacc gtgaggcgga cttggcgcag gatatgcgca atatcgtggc
    15541 tgctattttg cgcgatgact acggtttgac tgttaaaacc gacggcacgg gcaaaggcaa
    15601 tatgccgctg cgcgaagcgg tcaagctgat tcgcggctcg gatgtggcga ttgagtttca
    15661 caccaacgct gccgtcagca aagcggcgac aggcatcgaa gccttgagta ccgt
//
LOCUS       DAWKQB010000035        15310 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.035, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000035 DAWKQB010000000
VERSION     DAWKQB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.035"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..1608)
                     /locus_tag="WJL14_000830"
     CDS             complement(13..1608)
                     /locus_tag="WJL14_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ7655929.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLTFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDNASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(1715..2110)
                     /gene="hisI"
                     /locus_tag="WJL14_000831"
     CDS             complement(1715..2110)
                     /gene="hisI"
                     /locus_tag="WJL14_000831"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ7655930.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(2141..2908)
                     /gene="hisF"
                     /locus_tag="WJL14_000832"
     CDS             complement(2141..2908)
                     /gene="hisF"
                     /locus_tag="WJL14_000832"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ7655931.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(2921..3658)
                     /gene="hisA"
                     /locus_tag="WJL14_000833"
     CDS             complement(2921..3658)
                     /gene="hisA"
                     /locus_tag="WJL14_000833"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ7655932.1"
                     /translation="MLLIPAIDLKEGHCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKRGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(3691..4329)
                     /gene="hisH"
                     /locus_tag="WJL14_000834"
     CDS             complement(3691..4329)
                     /gene="hisH"
                     /locus_tag="WJL14_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ7655933.1"
                     /translation="MQTAIIDYGMGNLHSVLKSVRTAGQLAGKNVEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLLDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(4460..5962)
                     /gene="pta"
                     /locus_tag="WJL14_000835"
     CDS             complement(4460..5962)
                     /gene="pta"
                     /locus_tag="WJL14_000835"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ7655934.1"
                     /translation="MAKVLIVPVSAGLDASAVAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGLDVDTVIE
                     ATKLAQEKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(6147..7205)
                     /gene="fbpC"
                     /locus_tag="WJL14_000836"
     CDS             complement(6147..7205)
                     /gene="fbpC"
                     /locus_tag="WJL14_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ7655935.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(7226..8743)
                     /gene="fbpB"
                     /locus_tag="WJL14_000837"
     CDS             complement(7226..8743)
                     /gene="fbpB"
                     /locus_tag="WJL14_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ7655936.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLNLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAVLTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(8811..9806)
                     /gene="fbpA"
                     /locus_tag="WJL14_000838"
     CDS             complement(8811..9806)
                     /gene="fbpA"
                     /locus_tag="WJL14_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ7655937.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(10057..10526)
                     /locus_tag="WJL14_000839"
                     /pseudo
     CDS             complement(10057..10526)
                     /locus_tag="WJL14_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(10583..11959)
                     /gene="argH"
                     /locus_tag="WJL14_000840"
     CDS             complement(10583..11959)
                     /gene="argH"
                     /locus_tag="WJL14_000840"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ7655938.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLIMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADEAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGSAPEQVRFQV
                     KRWREMSA"
     gene            complement(11978..12847)
                     /gene="galU"
                     /locus_tag="WJL14_000841"
     CDS             complement(11978..12847)
                     /gene="galU"
                     /locus_tag="WJL14_000841"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ7655939.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPHITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAQKGALKQMVEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(12875..13474)
                     /gene="rdgB"
                     /locus_tag="WJL14_000842"
     CDS             complement(12875..13474)
                     /gene="rdgB"
                     /locus_tag="WJL14_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ7655940.1"
                     /translation="MSEKPEKIVLASGNVGKLEEFGNLFKPYGITVLPQSEFGIPECP
                     EPYSTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDHPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(13467..13700)
                     /locus_tag="WJL14_000843"
     CDS             complement(13467..13700)
                     /locus_tag="WJL14_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655941.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLFGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGALALYLRHKSLRAAPSSSQGNRYV"
     gene            complement(13808..14341)
                     /locus_tag="WJL14_000844"
     CDS             complement(13808..14341)
                     /locus_tag="WJL14_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ7655942.1"
                     /translation="MADFNQILTTGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGHAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(14443..14901)
                     /gene="nudB"
                     /locus_tag="WJL14_000845"
     CDS             complement(14443..14901)
                     /gene="nudB"
                     /locus_tag="WJL14_000845"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ7655943.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPKGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPITLQPEEHVSYGWFDMEEAAEKVFSSSNRCAILELGRFLGKR"
     gene            15111..15187
                     /locus_tag="WJL14_000846"
     tRNA            15111..15187
                     /locus_tag="WJL14_000846"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15145..15147,aa:Pro,seq:ggg)
BASE COUNT         3031 a         3744 c         4768 g         3767 t
ORIGIN      
        1 gccgattgct ttttaaagtt tgaccgaatg ctcgcgcgtt tcgtggaaca cgatgtccgg
       61 ccagcgttct tgcgtgagtc ccagattcac gcggttaggg gcgaggtagg cgaggttgcc
      121 gcccgcgtcg atggcgaggt tgcccgcgtt ggctttttca aattccgcca gttttttctt
      181 gtcgtcgcac gatacccagc gcgccgacca gatggatgcg ttgtcgaaca cggcttccac
      241 gccgtattcg ttggcaaggc gcgaggtaac gacttcaaac tgcaacacgc cgaccgcgcc
      301 caaaatcaaa tccgcgccgc tcattggttt gaacacctgc accgcacctt cttcgccaag
      361 ctgttgcaaa cctttttgca gttgcttgat tttcagcggg tttttgatgc gaacgctgcg
      421 gaacagttcg ggcgcgaaga atgggatgcc ggtaaacgtc agttgttcgc cttcggagaa
      481 gctgtcgccg atttggatgt tgccgtggtt tgggataccg ataatgtcgc cggcgtaggc
      541 ttcttcaacc agctcgcggt cgtgggacat gaaggttacc acgctggagg cggcgatttc
      601 gcggttgata cgcaggtgtt tcattttcat gccgcgctcg aatttgccgg agcagacgcg
      661 caagaaggca atacggtcgc ggtgtttcgg gtccatattg gcttggattt tgaagataaa
      721 tccggaaaac ttcggctcgt ccggctcgac catacgcacg gtcgcatcgc gtggtttcgg
      781 cgcgggcgcc cattcaatca atgaattgag gatttcctga ataccgaagt tgttaatcgc
      841 agagccgaag aatacgggcg tgagttcgcc ggcgaggaat tcgtcgagat tgaactcgtt
      901 ggaagccgcc tgcaccaatt cgatttcgtc gcgcaactgc tgtatttcta acggaaagcg
      961 ttgttccaat tcgggattat cgatgccttt gatgatgtcg aactcgtgcg gcaagcgttc
     1021 gccgcccgct tcaaagagat agatttcgtc gttcaggatg tggtacacgc ctttgaagtt
     1081 tttgcccatg ccgatcggcc aggttacggg cgcgcagcgg atttgcagga tgttttccac
     1141 ttcgtccagc aattccaggg aatcgcgcac ttcgcggtcg tatttgttca tgaacgtaac
     1201 aatcggcgta ttgcgcaggc ggcagacgtt caagagtttg atggtttgcg cttccacgcc
     1261 ttttgccgcg tcgatgacca tcaaggcact atcgacggcg gtcaaaacgc ggtaggtgtc
     1321 ttcggagaag tcttggtgtc ccggcgtgtc caaaaggttg acggtgtggt ctttatagtc
     1381 gaactgcatc acgcttgatg ccacggaaat gccgcgctgc ttctcgatgt ccatccagtc
     1441 ggaggtggcg aatttgccgg ttttcttgcc ttttaccgta cccgcgcttt gaatcgcacc
     1501 tgaaaacagc aagagttttt cagtcaacgt ggttttacct gcgtcagggt gggagatgat
     1561 ggcaaacgtg cggcggcggc gcacttggtc gaggatttct tgggacatgg ttttctttgc
     1621 aaaaaggttc aggccgcttt tcagacggcc cggacagtgt ttgagacggc gaaattgtac
     1681 aaaaaaatgc ctgataattc aatgttggag gcggtcagtg cgtgctgccg taaatctctt
     1741 tttcgtcttt caggacggca tcggcggttt cccacgcgcc gccgcgccag actttgtaaa
     1801 agcagctttc tcgcccggtg tggcaggcga tgccgccatt ttgggcgatg agcatcacaa
     1861 tggcgtcgcc gtcgcagtcg aggcgcagtt cgcggacttt ttgcgtgtgt cccgactctt
     1921 cgcctttcat ccattgtttt tggcgcgaac ggctgtaata gtgggcaaag ccggtttcga
     1981 cggttttttg cagggcttcg gcgttcatcc acgccaccat taaaatacgt ttggtttcgg
     2041 catcttgggc gatggcgcaa accaaacctt tttcgtcaaa tttgacggct tcaagcaggt
     2101 ttttatccat attttctttc agacggcatg gtcggggcgg tcagaggcgc acttcgatgc
     2161 cggcttcgcg catagcgcgt ttggcttcgc ggatggcgat ttccccgaaa tggaaaacgc
     2221 cggcggcaag tacggcatcg gctttgcctt cggttatgcc ttcaatcagg tgccggacat
     2281 tgccgacccc gccggaggcg atgacgggga tgtcgacggc ttcggcaacg gcgcgggtca
     2341 gcggcaggtt gaaaccctgt ttcgtaccgt ccctgtccat accggtaagc aggatttcgc
     2401 ccgcgccgcg tttttgcatt tcgatcgccc attccaccgc atccaaaccg gtcggattgc
     2461 gcccgccgtg ggtaaagatt tcccagcgtg tgttttcggg gttgacggct ttggcatcga
     2521 cggcggcgac gatggcttgc gaaccgaaaa atccggcggc ttcgtcaatc aggtcgggac
     2581 gggtaacggc ggcggtgttg atgctgactt tgtccgcgcc ggcattgagc aggcggcgga
     2641 tgtcggcaac ggtgcgtacg ccgccgccga cggtcagggg gatgaagact tgtccggcaa
     2701 cttcttcgat gatgtgcagg atggtatccc ggttgtcgga tgaggcggtg atgtcgagga
     2761 aggtcaattc gtccgcgcct tcgccgttgt agcgtttggc ggcttcgacg gggtcgcccg
     2821 cgtcgcgcaa accgatgaag ttcacgcctt tgacgacgcg cccgtctttt acgtcgagac
     2881 aggggatgat gcgttttgcc agtgccataa tcggatgcct ttagtcgagg gaatctgcca
     2941 gttgctgcgc ttgggcaaaa tcgatgctgc cctcgtaaat cgcgcggccg gtaatcgcgc
     3001 ctgctactcc acgtttttcg gcggcacaca gggcgcggat gtcgtccaag ccggtcagtc
     3061 cgccggaggc gatgacggga atgcggacgg cttgggcgag tttgaccgtc gcgtcgatgt
     3121 tcacgccgct catcataccg tcgcgcccga tgtcggtgta gatgatgctg ttgacgccgt
     3181 cgtcttcaaa gcgttttgcc aaatcaatta catgatgccc ggttacggtt gcccagccgt
     3241 cgatggcggc cataccgtct ttggcatcca gcccgacaat aatcctgccg gggaaggctt
     3301 tgcacgcctc gcgcacgaag tcggggtttt tgactgccgc cgtgccgata atcacgtcgt
     3361 ttaagcccaa atccaaatat tgtccgatgg ttttcaaatc gcgtatgccg ccgccgagct
     3421 gtacggggat gtctttggcg acggcggcga ggatgtcttt gatggcgggc aggttttgcg
     3481 gaacgccggc aaacgcgccg ttcaaatcca ccagatgcag gcggcgcgcg ccctgtttga
     3541 accagtgcag cgcggtttcg gcgggcgaat cggaaaagac ggtcgcctct tccatcagcc
     3601 cttgtttcag gcggacgcag tgtccttctt tcaaatcgat ggcgggtatc agcagcatag
     3661 tttttctcct tgtgcggggc cgtgtccggc ttaccagttt aaaaagtttt tcaacatcgt
     3721 cagcccggca tcgtggcttt tttcggtgtg aaattgcgtg gcgaatacgt tgtctttgcc
     3781 gacgatgcag gcaaacgggg acgggtagtc gctttcgccc aatatggttt cgggattttc
     3841 gggggcgaaa tagtagctgt ggacgaagta aaaacgcgtg tcttggggaa tatctttaaa
     3901 cagcgggtgg ttttgggttt ggcgcacggt gttccagccc atatgcggga ctttcagacg
     3961 gcatccctgc gggtcgagga ggtcgcgctc aaagcgtctg actttgccgc cgaaccagcc
     4021 caagccgtcg gtgtttcctt cttcgctgtg gtcgaataaa agttgcgcgc cgacgcagat
     4081 tccgaaaaag ggtttgtttt ttaaggcatc tttgactgcc tcgtccaggc cgccgcgcgt
     4141 cagtgccgcc atacagtcgg gcatcgcgcc ctgcccggga aaaatgactt tgtcggcgcg
     4201 ggacacgcgt tcggggtcgc cgcttaaaaa gatttcgaca ttttttccgg caagctgccc
     4261 tgccgtccgg acggatttca atacggaatg caggttgccc ataccgtaat cgataatggc
     4321 ggtttgcatg gcttcctcct cttttttgca atatggctgc gattttaaca aacaaatgtg
     4381 ccgggctgat aaaaatgccg tctgaaaacg ggagtctgtc ttcaagacgg catagggttc
     4441 aaacccggaa agccgtttgt cagccttcca tttgttttgc ctgaacggca gtcagggcga
     4501 tggtgaacac gatgtcttcc accagcgcgc cgcgcgacag gtcgttgacc ggtttgcgca
     4561 ggccttgcag cagcgggccg acgcttaaga cgttggcact gcgttggacg gctttatagg
     4621 tgcagttgcc ggtgttcagg tcggggaaga ccaaaacggt tgcctgtcct gccaccgggc
     4681 tgcccggagc tttggatttg cccacacccg gcacggttgc cgcatcatat tgcagcgggc
     4741 cgtcgatggc gagatcgggg cgtttttctt gggcaagttt ggttgcttcg atgacggtat
     4801 cgacatcgag accgctgccg gagttgaccg tagagtagga aatcatggcc actttcgggt
     4861 cgatgccgaa ggcttttgcg gaatcggcag actggatggc gatgtcggca agctgttgcg
     4921 cagtcgggtt cggattaacc gcgcagtcgc cgaagacgag gacttggttg ggtagcagca
     4981 taaagaatac gctggatacg aggcttgcac ccggtgcggt tttaatcagt tgcaaagcag
     5041 ggcggatggt gttggcggtc gtatgaacgg caccggatac caggccgtcc acatcgtttt
     5101 gcgccatcat catcgtaccg agtaccacgg tgtcttgcag ttgcttgcgc gcgtcttcgg
     5161 gtgtcaggcc tttggatttg cgcagttcgc acatcggctc gacgtattgt tcgaccaatg
     5221 aggcgggatc gatgatttcc aaagagtcgg gcaggctgat gccgcgttct ttggcaacgg
     5281 cttcgacttc ttcgcgtttg gcaagcagga cgcagcgggc aatgcctttt tcgtggcaga
     5341 tggcggcggc ttggacggtg cggggttctg cgccctcggg caggacgatg cgtttgtcgg
     5401 cttggcgggc gaagtcgatc aggttgtagc ggaattgcgc cggcgacagg cgttttgctt
     5461 cgcggcctgc caatacggat acgtctttca gcgcgtcgct cgaaccgaag aaggtcaggc
     5521 cggttttttc ggctgccgct tcggcaacgg aggctgccgc gccgtccacg acaaaacctt
     5581 ccaatacgcc cggcgcggcg gcgaagaact gtttggcaag gttcagccga tttgccagtt
     5641 cgtcggcatc ggcgttgtcg gaacggacgg cgaagacggc tgccgcgtca agggacaatg
     5701 ccagttcgac gtttttgcct gcgaggtaga ttttgtcggc atcgggcgcg atgccttcga
     5761 tgacgaggtt ggcggcatcg agtgcggcaa ctttgccgac cagtgcgtcg aaccagtcgt
     5821 cgcttttgcc ttgcgcgagc agggtttcgg cggttgcgtc aacggcttgg aaaatttgtg
     5881 cgtccagtgc ttttgcaaag gcttgtgcga cggcggaggc gtccagtccg gcagatacgg
     5941 gtacgatgag tacttttgcc atgatatatc ctttcgtatg ctgcggtgtg cggcatatgt
     6001 ggttggaagg ggcggcatat aggcagaaac ggctgcctgc gtgccgtgcg tgccgtgttt
     6061 ggcttgaggc gcgcaggttg aatatagcaa acaaattctg tttccaacaa gataaatatc
     6121 cgcaggtttg tggatgctgc cgcctttcag agggtatttc cggggaagaa cagggcggga
     6181 ccgtccaaat ggaggacggc ggaaatgccg tctgacaggg tgggggcgga agggaggttg
     6241 agcgtgagga cggtttgtcc gacccggaga ctgatttcgg tatgccgcgc tttgggcgtg
     6301 gttttgagaa ccacggcgtg aatggaggcg gtgggtgcgg aatgggggtg aaggctgaac
     6361 tgttccggac ggatgagcag tgtgccgcgt gtgcctgcgg gtgcgccgct ttgaacgggc
     6421 aggcggccca atccgcaatc ggcggtgccg tcggcgttga gcgcggcggg gaacacgatg
     6481 ccttcgccga taaacagggc ggcatcaagg tcggcaggtt gtcggtacaa ttcgtgaggg
     6541 cttgcagttt ggaggatgcg cccctgtttc atcacggcaa tccggtcggc gtattgcagg
     6601 gcttcttcgc ggtcgtggct gacgaaaacg gcagatttgc cgttggcgcg cagggcggca
     6661 atcatgtctt cgcgaatctg gcggcgcaac tgttcgtcca gcgcgctgaa gggttcgtcc
     6721 aacaaaatca gttcgggatc gggtgcgagg gcgcgggcga gggcgacgcg ctgttgctgt
     6781 ccgcccgaaa gttcgtgcgg atagcgtccg gcaagttcgg aaatgccggt caattccaac
     6841 atagcttcga tgcgctgccg ctcttgcgcc gtcttgcctt tgccgttgcc cagcccgtag
     6901 gcggtgttgc ggtaaacggt caggtggggg aacagcacac cttcctgtac gacataaccc
     6961 aaacggcgtt cgcggacggg gaggttggta tttttcgaga agatggttct gccggaaagc
     7021 gaaatttcgc caaaatcggg ttgttcaaaa ccggcaaggc agcgtaaaag ggtggttttg
     7081 ccgcagccgg acgcgccgac gataaagagg atttcgcccg ggtcgaggct gagcgaaatg
     7141 tcgtttaaaa ccggggtgtt ttgaaaactt ttggacaggt gtccgatgtg cagggcggcg
     7201 gtcatggcgg tacttcctca agctgttatt tgaaagcgta tttcttcagc aggaatacgg
     7261 ggatgccgga aaataatacc agcatcagcg cgtaaggggt ggcggcggcg tattgtgcgt
     7321 ccgatgtgta ttcccaaacg gcggtggaga gtgtgtggac atcgtcggtg gtcagcagca
     7381 gggtggcggt cagctctttc atcagtttga ggaagacgag tgcgaatgcg gcggtaatgc
     7441 cgggcaggat ggacggcagt accaacgtcc tgaaaataaa gaagtgtccg cgccccaatg
     7501 ttgcgccgac ctgttccatc ccttttggga gttgttccaa ggaagtcctt agggtggttt
     7561 gcgccatcgg caggtaaagc atgaaatagg caaggatgac gacgataaag gtttggtaaa
     7621 cggcaggggt gtagttgatg ctgaaataaa ccaaggatag ggcgataacc aaaccgggga
     7681 cggcgtgcag taaaaacggc agcctgtcta tccaaacggt taaaaaattg cgatagcgga
     7741 ccgatgccca aacaaggggc aaggcacata atatagtcaa aaccgcacct aaagccgata
     7801 cgcttaagga acggataaag gcatcaaata cggatacgag cgcgaatgtg ccggaagtgc
     7861 cgaccatcat ccaatgtatc aatacgccaa aggggataat aatgcccaaa gtcaacaagc
     7921 tgcttaaaaa aacaatcgcg ccaatctgac cggggaggtt gagggttttg acgggataag
     7981 gacgggcaac gcctttgccg ctgtggtaaa tcttggcttt gccgcgaaat atgctttctc
     8041 caaatacgac gatgccgcac accgccatta aaacagcgga aagcagggcg gcggtattgt
     8101 tgttgtagga catttcgtat tcttggaaaa tggcggtggt aaaagtgggg tagttcaaaa
     8161 tggataccgc gccaaattcg accagcatat gcagggcaat cagtaacacg ctgctgccga
     8221 tggcgggttt gagctggggg aggatggcgg aaaaaaaggt ttgcaggcgg cttttgccca
     8281 aggacaggct gacttcttcg taagacaggc tgatgcgttt gagtgccgcc tcgacgggca
     8341 ggtaggcgag cgggaacgag gacaggctca taatcatcac tgtcccccaa aagccttcga
     8401 cacggaaggt caggctgatc caggtgaaac agctgacaaa tgcggggatg cacaaaggca
     8461 gggtgattgc cgtctgaaaa aaggttttgc cgaagaagcg gtaacgttgg aacaaaaggg
     8521 cgcaggcaat gcccaaaaca atggaaatca gggtaacgcc cgccatcatc gtcaaggtgt
     8581 tggagagcaa atcccacata cgcgggcgga acaacagttc gacggcgcgg ttgatgccga
     8641 cctgccacga acgcatagcg acatataaaa aaggcagggt aagcggtagg gcaatcagta
     8701 ggatgaggcc ggtaagccaa atgggtattt ttttaggaga catagtgttt ttcatcggca
     8761 aaacgggcgg acagtataaa tgtccacccg tttgacaatc cgaaaacggc ttatttcata
     8821 ccggcttgct caagcagccg ggtggcgtgt tctttttcgg aaacagtggt ggcggacact
     8881 tggggtgctt ccaacttggc gatgggttcc aaattgaaag tggataccac gtgcggattc
     8941 aaaggatatt cggcacggac ggcggtcagg gcgcgctgtc cttccttgct ggcgaggaag
     9001 gcgacgaatt ttttcgcctc atccttgttt tgggaggatt ttaacacggc tgcgccggaa
     9061 taggtaatga gtgcgccggg atctctgtgg cggacgaaat tcaggcgggt gtggacattt
     9121 tgtacgcctt tttcacgcgc gaaagcgtgc cagtagtagt tgttgatgag ggcggcatcg
     9181 atttcgccgt tttcaaccgc ttgaagggcg acggagtttt tagcgtaagg cttgccgtat
     9241 tctttcagac ctttgagcca tttcaatgcg gccgcttcgc ctttcagttt gacgatggcg
     9301 acaacctgtt ccaagaacgc gccggaagtg ggggcgtaac cgatgcggtt tttccatttc
     9361 ggcgtggcgt aattcaggac ggatttttcc aaatcttttt cagacagttt gcgggtgtcg
     9421 taaacgacga cgcgcgaacg tccgctcagt gccacccagt cttttttggc ggcaaccggc
     9481 acgcccttgc cgcgtgtttc gttgatggtg gaggcgggca ggggctctag gaggttggct
     9541 gcggaaaggg tggcgagtgc cgggatttgt tcggaataga atacgtcggc ggggcttcgg
     9601 ctgccttctt ctttgatttg accggcaagc tggtcgcctt tggcgctgtt gagtttgact
     9661 ttgatgccgg tagcccgggt aaaggcatct gcaacggctt gtgccgcttc tttgtgttgg
     9721 ccgttgtaca cggtaatgtc tgccagcgcg ggggttgcgg cggtcagggc tgcggcaagc
     9781 agtgcgtatc ggatagatgt tttcatatcg attttctcct aatgaatgag agtgtacacc
     9841 ttgttaaaac ataacggtgt gtagtgtatt ccttcttctt tataaatgca aataattatt
     9901 ttttaaattt gttattatcc gatccggtta tggtttgttc tgacttgtat tttttccgtg
     9961 catcgcgccc gtaaggcgga agcggcgggc aatgcgtggc ggaatgtggg taaaggcggc
    10021 attttgattt gtcggaatgc ttgagaaccc ctctctttaa aacagcgtct gaacagggtt
    10081 gccggaatag tattgccatc ccggcagata cagtttgtcg gggtcttgcc aatattgttc
    10141 atccagactg ttcggcagtg aggcggtttt gttgtcgaga tgttttgtta tatcccacat
    10201 ttctttcagg tttttacctt ccgattggag gtggcggatt tcttttctca atgcgtctgc
    10261 cgcccgatgg atcagcattg cgctgtgtat cgcgccgatt ttttcagaac ggaacaagtc
    10321 ttttcggcgg cgggaaaacc ccaattgcag acaaattgca gtatgccgcc gttgccgatg
    10381 tcggcttccg cccgccaaat caaaaccagc tcctgttcac gtccgtccat gctttcgagt
    10441 ttgccgtcat gctgttcaaa aagtttgtct accgcctgcc acatcatctg ttcgaagcgg
    10501 tcggcttggg tatccgtatc ggtcatggtg ttcttgtctt ttaaaaatgc cgtctgaaca
    10561 tttcttcaga cggcatcggg ggttaagctg acatttcccg ccagcgtttg acttggaaac
    10621 ggacttgttc aggcgcgcta ccgcccaagt ggttgcgcgc gtttaagctg ccttcgggtg
    10681 tcagcacgcc gtaaacgtcg tcggcaatca aatcgctgaa accttgtaag acttcgagcg
    10741 gcagttcgct caaatcgacg ccggcttcat cagcatggcg cacggcttgg gcgacgactt
    10801 cgtgggcatc gcggaaaggc atgccttttt ttaccaagta atctgccaaa tcggtggcgg
    10861 tagcgaagcc ctgcatcacg gcggcgcgca tattgtcggg tttgacggtt acgccgcgca
    10921 tcatatcggc gtaaatccgc aacgtgtcga taagcgtgtc ggcggtgtcg aacaagggtt
    10981 ctttgtcttc ctgattgtct ttgttgtacg ccaagggttg ggatttcatc agggtaatca
    11041 gaccgataag gtgtccgatg acgcggccgg atttgccgcg cacgagttcg ggcacgtcgg
    11101 gatttttctt ctgcggcatg atggacgaac ctgtgcagaa acggtcggcg atgtcgataa
    11161 agccgaaacg cggactcatc cacaaaatca attcttcgga caggcggctc aggtgaacca
    11221 taatcagcga ggcggcggcg gtgaactcga cggcaaaatc gcggtcggat acggcatcga
    11281 gcgagttttg gcagatttgc tcgaaaccca gtaattcggc ggtaatttcg cgccgaatcg
    11341 ggtaggtcgt accggcaagg gcggctgcgc cgagcggcat acggttgacg cggcggcggc
    11401 agtccgccat ccgttcgtta tcgcggccga gcatttcgac gtaggcgagc atatggtgtc
    11461 cgaagctgac gggctgggcg acttgcaggt gggtaaagcc gggcatgacg gtttcggcgt
    11521 tttgttccgc caaatccagc aatgccgtct gaaggctttg aatcaggctt tggataacgg
    11581 taatctggtc gcgcagccaa aggcggatgt cggtggcgac ttggtcgttg cggctgcggc
    11641 cggtgtgcag gcgtttgcct gcatcgccga ttttgtcggt caggcggcgt tcgatgttca
    11701 tgtggacatc ttccaaatcg gacgaccatt cgattttgcc gctgcggatt tcttcgagga
    11761 tttccgccat gccccgacgg atgtccgcca aatcgccttc gtccaacacg ccggtttctt
    11821 tcagcatttg cgcgtgggca agcgagcctt ggatgtccca ttcggcaagg cgtcggtcga
    11881 aaccgatgga ggcggtgtat tgtttgacga gttcggaaac gggttcgttg aaacgtccgg
    11941 accaggtttt gtcgtgcata aggattcctt gatggggtta ttcggtacgg tatttttcca
    12001 aaaggcggcg gaagggttcg ccggtttcgg ggtgtttcag gccgtaggcg acggtggctt
    12061 ccagatagcc cagtttgctg ccgcagtcgt agcgcgtgcc ttcaaagggg tgcgctagga
    12121 caaattcgtg atcgagcagc ttggcgatgc cgtctgtaag ctggatttcg ttgcccgcgc
    12181 cgcgcggcag tccggtcagt aagtcgaaaa tgcgcggggt aaggatgtag cgtccgacga
    12241 cggcaaggtt ggagggcgcg tcttcgggct tgggtttttc gacaatgccg gtaatgcgtt
    12301 ggaactgttt gagctgttcg gtttcgacga tgccgtatga gccggtttgc gacggttcga
    12361 cagtttctac gcccaaaatg ctgttgccgc tgcgttcgta cacttcgacc atttgtttga
    12421 gcgcgccttt ttgggcatcg attaagtcgt cagccaaaat cacggcgaag ggttcatcgc
    12481 cgatggcggc gcgggcgcac aagacggcgt gtccgagccc cagtgcttcg gcttggcgga
    12541 tgtagaggca ggtaatatga ggcggcagga tgttgcggac gtgttccaga agtttatctt
    12601 tatgacgcat ttccaactcg gtttcgagtt cgtatgcctt gtcgaaatgg tcttcgatgc
    12661 tgcgtttgtt gcgtccggta acaaacacca tttccgtgca gccggcttcc acggcttctt
    12721 ctacggcgta ttggatcagc ggcttgtcga cgatgggcag catttctttc gggctggcct
    12781 tggtggcggg caggaagcgt gttcccatcc ctgcgacggg gaagacggct ttccgtatgg
    12841 gtttcattct ttttcctttg tattgttttg atgtttaaag ggcgagtttg cgtaagagtt
    12901 cggcaagtgc ctgcgcgcgg tggctttcgc ggtttttgac ctccgaatcc aattcggcgg
    12961 cggttttgcc gtgtccgggc agataaaaat acgggtcgta accgaaaccg ttttcaccgg
    13021 cgggaacgcc gttccattgt ccgtgccata cgccctcggc gataatcgga cgcgggtcgt
    13081 ctttatggcg gacaaaaacc aatacgcaga cgtagcaaca gcttttgtcg gctttgccga
    13141 caagttcggc ggcaagtttc aggttgttgg cggtatcgga tttgggatgg tcgcccgcgt
    13201 aacgtgcgga atgtatgccc ggtgcgccgt ttagggcggc ggcacagatg ccgctgtcgt
    13261 cggcgagtgc gggcagcccg ctgtattcgg cagcatggcg tgcttttgcc agcgcgtttt
    13321 cgacaaaggt ggaatagggt tccggacatt cgggtatgcc gaactcggat tgcggcaata
    13381 cggtgatgcc gtaaggtttg aataggttgc cgaactcttc gagcttgccg acattaccgc
    13441 ttgccaaaac gattttttcc ggtttttcag acatagcggt ttccctgtga tgaagatggg
    13501 gcggcgcgta gggatttgtg ccgcaggtag agggcgagtg cgccgatttg cccgaacagc
    13561 gcgccgaatg caaaaaggaa ggaggcgacg gcgaaggctt tgctgccgac ggtcagcaga
    13621 taagcaccaa agaggacgag cagcataaat gccagtgtga agagggcaac agacaacaga
    13681 tagatttttc ggcggttcat ggcgttcggt cggaaacggt atgttcggat tatatccgat
    13741 tgggacggta ttccctagag cttggaaaaa tgccgtctga aggggcgttc agacggcatg
    13801 gcgggcgtta tgcctgtttg ttccaacgtt cgatggattc tttgatgact tttttcgctt
    13861 cttccgcatc gccccaagat tcgactttgg tcgtacctgc ttttttcagg tctttgtaat
    13921 ggttgaagtg gaactcgatt tgtttgatga gctgttgcgg caaatcggac aaagttttgt
    13981 aggcgtggcc gttgttgcgg tcgtcggcag gaacgcagac gattttgtcg tctacttcgc
    14041 cgtcgtcaac gaatttcatc acgccgataa cgcgcgcttc caagaatacg ccggttgcca
    14101 gaggttgttc ggtaacgagc agcacgtcca attcgtcgcc gtcttcgtcc aaagtttggg
    14161 gaatgaagcc gtagttggtc ggtttggcga agatggcggg ttcgacgcgg tcgagttgga
    14221 atgcggcgag tttgcggttc cactcgattt tgtggttgct gccggcgggg atttcgttga
    14281 cgacgttgat gatgccgccg tccacgtcgc cggtggtcag gatttggttg aagtctgcca
    14341 tttggtttcc tttatttggg aaagtttgaa gtttgaaagt atagcacaaa cgtccggctg
    14401 gaaatgcgcc cgatgccgtc tgaaagggtg tacgggcgcg tgttaccgtt tgcccaaaaa
    14461 cctgcccagt tccagaatcg cgcacctgtt ggacgaggaa aacacttttt ccgccgcttc
    14521 ttccatatcg aaccaaccgt aggagacgtg ttcttcgggt tgcagggtga tgggcgtatc
    14581 acgcgggatt tcggcagaga agaggtgttc gcggttttca aacacgcctt ttggatagcg
    14641 gtgccgccag tggtggtaga tttcgtaaac cgtgctgtcg tgccagtctt gaagctgccc
    14701 gtccgccagc aggatgccgg tttcttccca aacttcgcgc cttgccgttt gggcgacggt
    14761 ttcgcccggt tcgaggctgc cggttaccga ctgccaaaat cctttcggat gcgtgcgttc
    14821 gatgagcagg atgccgccgt ccccgctata aaggacgacc agtgcggaaa cggggtattt
    14881 gagcggtttt gccatcggca tctttcggcg ggctgcggta atgaaggggc ggattatagc
    14941 aaacgcgcac gttatggcgt ttatcctttt ccgtatcctt ttttctgcac ggatgggacg
    15001 cgccggtgtt tgccggtaaa ttttccgttg tgtcaaaaag ataagggcgg ttgtgatttt
    15061 aatgcttgcc aaagcgtcgg gcggaaacta taatccgaaa cttatcgagt cggagtgtgg
    15121 cgcagtctgg tagcgcactt gcatggggtg caaggggtcg aaggttcgaa tcctttcact
    15181 ccgaccaaaa attccgaaag ccgctttcaa aagcggcttt tttgccgtcc gtatgattat
    15241 gatgtagagt acgcggcgac agacattcaa ataccgtccg aaaaccgttc agatggcatc
    15301 tctttatctt
//
LOCUS       DAWKQB010000036        15019 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.036, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000036 DAWKQB010000000
VERSION     DAWKQB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.036"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            335..1489
                     /gene="obgE"
                     /locus_tag="WJL14_000847"
     CDS             335..1489
                     /gene="obgE"
                     /locus_tag="WJL14_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ7655944.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEARARTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1572..2621
                     /locus_tag="WJL14_000848"
     CDS             1572..2621
                     /locus_tag="WJL14_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7655945.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3309..4730
                     /locus_tag="WJL14_000849"
     CDS             3309..4730
                     /locus_tag="WJL14_000849"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ7655946.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGKLTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNDAQL
                     AGRLKALGGIIGLLQRDPIEFLQGGAVSDGLSNEEIDDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4813..5592
                     /gene="xth"
                     /locus_tag="WJL14_000850"
     CDS             4813..5592
                     /gene="xth"
                     /locus_tag="WJL14_000850"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7655947.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5579..5923
                     /locus_tag="WJL14_000851"
     CDS             5579..5923
                     /locus_tag="WJL14_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655948.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPMLVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVRTLLSVPAA
                     LLIAFTLQDLLK"
     gene            5933..6463
                     /locus_tag="WJL14_000852"
     CDS             5933..6463
                     /locus_tag="WJL14_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ7655949.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQRQADLWNARLLVSPHSGSLNGIL
                     GGWQWGMKLMQEMLLA"
     gene            complement(6556..7671)
                     /gene="asd"
                     /locus_tag="WJL14_000853"
     CDS             complement(6556..7671)
                     /gene="asd"
                     /locus_tag="WJL14_000853"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ7655950.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANDVAELAKMDIIVTCQGGDYTKSVFQPLRDSGWNGYWVDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAIHAKVADELADPSSAILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPVSEIEAILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7847..8428
                     /locus_tag="WJL14_000854"
     CDS             7847..8428
                     /locus_tag="WJL14_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7655951.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8490..9344)
                     /gene="folD"
                     /locus_tag="WJL14_000855"
     CDS             complement(8490..9344)
                     /gene="folD"
                     /locus_tag="WJL14_000855"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ7655952.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9567..9644
                     /locus_tag="WJL14_000856"
     tRNA            9567..9644
                     /locus_tag="WJL14_000856"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9602..9604,aa:Pro,seq:tgg)
     gene            9669..9745
                     /locus_tag="WJL14_000857"
     tRNA            9669..9745
                     /locus_tag="WJL14_000857"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9703..9705,aa:Arg,seq:tct)
     gene            9773..9848
                     /locus_tag="WJL14_000858"
     tRNA            9773..9848
                     /locus_tag="WJL14_000858"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9806..9808,aa:His,seq:gtg)
     gene            10059..10565
                     /locus_tag="WJL14_000859"
     CDS             10059..10565
                     /locus_tag="WJL14_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ7655953.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10562..12340
                     /locus_tag="WJL14_000860"
     CDS             10562..12340
                     /locus_tag="WJL14_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ7655954.1"
                     /translation="MTVLKPSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12453..13277
                     /locus_tag="WJL14_000861"
     CDS             12453..13277
                     /locus_tag="WJL14_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ7655955.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAYIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14040..14828)
                     /locus_tag="WJL14_000862"
     CDS             complement(14040..14828)
                     /locus_tag="WJL14_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ7655956.1"
                     /translation="MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRR
                     AGCGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         3605 a         3790 c         4164 g         3460 t
ORIGIN      
        1 tgttccattt taaaacaatt agtaaaaccg agtttatcct agttatctag gacagcccca
       61 aaacttatta tatgtaacgg tttgaacgga cattccgtcg ggtcggaatg tcaaaaaggc
      121 gcgattgtac caaagaagtt ggacataatt ttgtttgcag gctgaagatt tgcttaaaaa
      181 ttcattaaga tgggcggaac aaatagtttg ggtacggcgt gttgaaatac cgcttatccc
      241 ttataatagc gtccgaaatt ctgttcagac ggcatcaaaa cacacggtaa tccgtctgaa
      301 ccccccattt gaaaccaaca aacaagagca ttgaatgaaa ttcatcgacg aagcaaaaat
      361 cgaagtcgcc gcaggcaaag gcggtaatgg cgcaaccagt ttccgccgcg aaaaattcgt
      421 accgcgcggc ggcccggacg gcggcgacgg cggcaaaggc ggtagcgtct gggcggaagc
      481 cgacgaaaac accaacaccc tcgtcgaata ccgcttcgtt aaacgctacc aagccaaaaa
      541 cggcgaaaaa ggccacggtt ccgaccgtta cggcgcaggt gcggacgaca tcgtcctcaa
      601 aatgcccgtc ggcaccctca tccgcgacct cgacaccgac gaaatcgttg ccgacctcac
      661 ttaccacgga cagcgcgtct gcctagccaa aggcggcaaa ggcggcttgg gcaacatcca
      721 cttcaaatcc tccgtcaacc gcgccccaaa acaatccacg cccggcgaag aaggcgaagc
      781 ccgttccctg caactcgaac tcaaagtcct cgccgatgtc ggcttattgg gtatgcccaa
      841 cgccggcaaa tccaccctga ttaccgccgt atccgccgca cgccccaaaa tcgccaacta
      901 ccccttcacc accctgcatc caaacttagg cgtggtacgc atcgacgaaa accacagctt
      961 cgtgatggcc gacatccccg gcctgattga aggcgcggca gaaggcgcgg gtttggggca
     1021 tcgtttcctc aaacacttat cgcgcaccgg attattgctg cacgtcgtcg atttggcgcc
     1081 cttcgacgaa accgtcaacc ctgccgaaga agcacttgct atcatcaacg aattgcgcaa
     1141 atacgatgaa gaactttacg gcaaaccgcg ctggctggtg ctgaataaac tcgacatgct
     1201 tgacgaagaa gaagcccggg cgcgcactgc cgccttcctc gaagccgtcg gctgggacta
     1261 ccccaaaccc gacgaccgtt tccaatttga catggaaacc ccgcgcctct tccaaatcag
     1321 cgcgctgacc caccaaggca cgcaggaatt ggtacaccaa atcaaccaat acctgaccga
     1381 gaaaaaacgt atcgaagctg aaaaagccga agcggagaaa gcagcggcaa atgttgaaat
     1441 tatcgaacag cagcctaaaa cggatacggg cgtgtttaag ccggagtgaa aaaagaaagg
     1501 gtttcagacg atatttgttc tacaaaaggt tgtttgaaac aattttaaag caagattgaa
     1561 atggattaaa aatgtcatct aaccaatata tccagtcgct tgagttgaca aactttaccg
     1621 ttcttccaaa cgatactttt gaattttctg aaaatctcaa tgttattgtt gccgaaaacg
     1681 gttgtggcaa aacacatttg ttaaaaattt tatacagcct gctagaagta acatctaaca
     1741 ccaaaaacag attactgaaa acagaattgc aaaaaagttt tgccgataaa ttactgaatg
     1801 ttttccgtcc cgattccttg ggcaggttga gcaaacgttt gcaggggcgc gggcgtacgg
     1861 aaatcgtttt aaaactgcaa aacggcacta cgcatagtag tctgaatttt tccagtaact
     1921 catcatcgca agtcaatgtt caaagcatcg gattgaaaga aaatgaatat actccgacac
     1981 ctatcttctt acccagccgc gaattaatta cactttgccc ttggttcact tctttgtatc
     2041 aaaaccaaag catccctttc gaagaaactt ggtttgatac ctgtatgcaa ttaaatcacc
     2101 cgcttgccaa aggccccaga gaaacaaaaa ttcgagaatt gctcgaacct attgagaacg
     2161 cgatgggcgg aaaagtatct gaagaacaag gaaggtttta tctatcgctg acaaatacgg
     2221 gcggtaagat tgaagcacct ttggtagcag agggtttgcg gaaatttgtc atgattgccc
     2281 ggttaatcgc aacaggggca ttgctggata aaggctatct attttgggat gaaccggaag
     2341 ctaatctgaa tcctaaattg atcaaagtgg ctgcccgtat tatttggtct ttatctcaac
     2401 agggtattca ggtttttatt gcaacacact ctttattctt attgagagaa ttggaacttt
     2461 tgaagcttga aaataacacg gatttacctg tacgtttttt ctcgttgatt gcttccgatg
     2521 atggaacaaa agtggagcaa gggaacagta ttaatgattt gaacacattg atattgttag
     2581 atgaaaacat tatgcaggca gacaggtatc tgaatatttg aggttaagct atgactacaa
     2641 tcacagaaaa gagattaacc tttgcttttc ctgaagatta ttacgttacc aagtatgacg
     2701 aatgggaaca ttataaaatt tttcagaaca gttgtaattt acgtaataaa attgatacaa
     2761 atgaaaaggg aaagaacggg ataaatcaaa gtgtagatga cgataatggc agctcaggtg
     2821 tagatataat agcctttcat gaatcgacac tttggctaat cgaaattaaa gattattacc
     2881 gacttgggct tgagccaaat gcccaatcaa tagatgaaaa actttctgac ctaccatatt
     2941 taatcgcaag aaaaattcga gattcattgg caggattggt ttctgctaaa tttaaagcag
     3001 caaaacagga agaaaaagat ttttctcgct tggctttaga ttgtaacgaa ataaaaatag
     3061 tctttcatat tgaaatgcct agtataagaa gtaaacttta tccctcgtca tcagatttag
     3121 caaacttatt aaaagataag tttaaattat cagaatttac aaaaaatttt gcaaactgtt
     3181 atgcagaacc aattttcaca aatatcagcc atattaataa tccgcaactg cgtaatgttc
     3241 cttggtccgt atctacagga acagaacaga aattatcatc cgagcaacaa agattaatcc
     3301 ataatcccat gaccaccatc tacaacaccc ttacccgcca aaaagaaccc tttgccccca
     3361 tcgaccctga aaacgtgcgt atgtacgtct gcggtatgac cgtttacgat tactgccatt
     3421 tgggacacgc ccgcgtgatg gtggtgttcg acatgattgc ccgttggttg cgcgagtgcg
     3481 gctatccgct cacttatgta cgcaacatca ccgacatcga cgataaaatc attgcccgcg
     3541 cggctgaaaa cggcgagacc atcggcaaac tgactgcgcg tttcatccaa gccatgcacg
     3601 aagatgccga tgctttgggc gtgttgcgtc cggatattga gccgaaggca acggaaaaca
     3661 ttccgcaaat gattgccatg attgaaaccc tgattcaaaa cggcaaggca tatcctgccg
     3721 caaatggcga cgtttactac gccgtgcgcg agtttgccgc ttacggacaa ctgtcgggca
     3781 aatcgttgga cgacctgcgt gcgggcgaac gcgtggaagt ggacggtttc aaacgcgatc
     3841 cgcttgattt tgtgttgtgg aaagcggcca aagcaggcga accggcgtgg gaaagcccgt
     3901 ggggcaacgg ccgtccgggt tggcacattg aatgctctgc catgagtgaa aacctgttcg
     3961 gcgatacctt cgacatccac ggcggcggcg cggatttgca gtttccgcac cacgaaaacg
     4021 aaattgccca aagcgtcggc gcgacggggc atacctgcgg tcatcatcac gcgcaaaccc
     4081 accacgggca aagcatcgcc agccacgtca aatactggct gcacaacggc tttatccgcg
     4141 tggacggcga aaagatgtcc aaatcgctgg gcaacttctt caccatccgc gaagtgttga
     4201 aacaatacga tccggaagtc gtgcgcttct tcatcctgcg cgcccactac cgcagcccgt
     4261 tgaactattc cgacgcgcat ttggatgatg caaaaggcgc gttgacgcgt ttgtatacga
     4321 ctttgaaaaa tacgccgccc gccgatccga tgccgtctga agccggcgat gactacacgc
     4381 gccgctttta tgttgcgatg aatgatgatt ttgatacggt gaaagcggtg gcggtgttgt
     4441 tcgaacttgc cggcgaagtg aacaaaacca atgacgcgca actggcaggc cgtctgaaag
     4501 ccttgggcgg catcatcggc ttgctgcaac gcgatccgat tgagttcctg caaggcggcg
     4561 cggtttcaga cggcctctcc aacgaggaaa tcgacgattt gatcgcccgc cgcaaacagg
     4621 cacgcgcgga taaaaactgg gcggaatccg accgcatccg cgaccttctg aacgaacaca
     4681 aaatcatttt ggaagacaac gccggcggca cgacttggcg gcgcggttaa aatccatcga
     4741 aaagggtaaa tgtgccgcat tttgcccttt tttgttcaga cggcattttt attgtgagaa
     4801 cttattttta ttatgcttaa aatcatttcc gccaacgtca acggcatccg ctccgcctac
     4861 aaaaaaggtt tttacgaata catcgccgca tcgggcgcgg acattgtctg cgtgcaggaa
     4921 ctcaaagcgc aggaggctga tttgtctgcc gatatgaaaa atccgcacgg gatgcacggt
     4981 cattggcatt gcgccgagaa gcgcggttac agcggcgtgg cggtgtacag caaacgcaaa
     5041 cccgacaatg tgcaaatcgg tatgggcatt gaagaattcg acagggaagg gcggtttgtg
     5101 cgttgcgatt tcggcaggtt gagcgttatc tcgctttatt tgcccagcgg cagcagcgcg
     5161 gaagaacgcc agcaggtgaa ataccgtttc cttgatgcgt tttaccctat gctcgaagcg
     5221 atgaaaaacg aagggcgcga cattgtcgtc tgcggcgact ggaacatcgc ccaccaaaac
     5281 atcgacctga aaaactggaa aggcaaccag aaaaattcag gtttcctgcc tgaagagcgc
     5341 gagtggatag gcaaggttat ccacaagctc ggttggacgg atatgtggcg cacgctttat
     5401 cccgatgtgc cgggctacac ttggtggagc aaccgagggc aggcgtatgc gaaagatgtc
     5461 gggtggcgca tcgattatca gatggttacg cccgaacttg ctgccaaagc cgtgtccgca
     5521 cacgtttata aagatgaaaa attttctgac catgcgccgc tggtcgtgga gtatgactat
     5581 gctgccgaat aaggttttgg gtaaatatga ttggaatgtg gacggaaaaa caggaatagg
     5641 cgcggcatgg gtcgtcgctg cgtttatcct gcccatgttg gtgtgggcga tatttatgtt
     5701 gtcgcggatg caaggctggc ttgcgccgac caaggcaaac ccgatatggg cgttggtgtg
     5761 gctgctgatt tgcctgccct gcctgctgat tgcgtccaaa tgtttgggtt ggaaaggctg
     5821 gcggctgttg gcaaacgttc ttgcctgcct cgccgtgcgc accctgttga gtgtgcctgc
     5881 cgcgctgctg attgcgttca cgctgcaaga cctgctcaaa taggcgcgga caatggaaag
     5941 tttcgagctt gaggatttga cgctgtggct ggtacgcgat gcggatgaag cggaaatgtg
     6001 gatagaccgc tgggcgatca gttatcccgt cgtgcagatg tccgaagcgt cggccggtca
     6061 aagtataggg gaatggcagg cagggcttca gacggcattt gaacgcatac gcggcaaata
     6121 cgtcgccgtt gtcgcacacg gtgcgggcgc ggccgcattt ttggcgtggc tgtatcaggc
     6181 agacatcctg acacggaaga aaattgccaa catcatcctt gtaccgcagc gtcccgatat
     6241 ttttcccgac gatgcggaac acgctttcca acgcgtccgc tgtccctgcc gtgccgcatt
     6301 ggttgtaccc gaacacggcg gcgtgccgca cggttgggcg caaagacagg cggatttgtg
     6361 gaacgcccgc ctgttggttt ccccgcattc gggcagtttg aacggtatac tcggcggctg
     6421 gcagtggggt atgaagctga tgcaggaaat gttgctggcg tgaacgcttg ccttatcgga
     6481 atttcggtta actggggttg caaggaaaat gccgtctgaa acaggcttca gacggcattt
     6541 ccattcaaac agggcttaca ggctgcccaa cacgatacgc agcacgcggc gcaacggctc
     6601 ggcagccccc cacaagagtt ggtcgccgac agtgaacgcg ctgatgtatt cgccgcccat
     6661 ctccagtttg cggatacggc cgacaggaac agacagtgtg ccggtaactt tggcaggagt
     6721 cagctcgtgt atgctggctt ctttttcgtt agggatgact ttcacccagt cgtttgcacc
     6781 tgccaaaatc gcttcgattt cggaaacagg caggtctttt ttcagcttca gggtgagggc
     6841 ttggctgtgg cagcgcatag agccgatgcg tacgcataag ccgtcgatta cggtcggatt
     6901 gtcgctgcgg ccgaggattt tgttggtttc cacaccgcct ttccattctt ctttagattg
     6961 accgttgccc aaatccacgt caatccacgg aatcaggctg ccggcgagcg gtacgccgaa
     7021 gttggctttc ggatagtctt cgctgcgcag gaaatcggac actttgcggt cgatgtcgag
     7081 aatcgcgctg gaaggatcgg caagctcgtc cgctactttg gcgtgaatcg cgcccatgcc
     7141 gctgatgagt tcgcgcatgt ttttcgcacc cgcaccggaa gcggcttggt aggtcatgct
     7201 ggttgcccat tcgaccaaat cgttttggaa cagaccgccc aaagccatca gcatcaggga
     7261 aacggtacag ttgccgccga tgtagttttt cacgccgttt ttgaggccgt tgtcgatgac
     7321 gttgcggttg accgggtcga ggacgataat cgcatcgtct ttcatgcgca gggatgacgc
     7381 cgcgtcaacc cagtagccgt tccagccgct gtcgcgcagg ggttggaaga cggatttggt
     7441 gtaatcgccg ccctgacagg taacgatgat gtccattttt gccaattcgg caacatcgtt
     7501 ggcatccaac aatgttttgg ctgcctgacc gaaatcaggg gctgcgccgc cgacgttgga
     7561 agtggtaaag aaaaacgctt caggaatgtg ggcgaagtcg ttttcttctt tcatacgctg
     7621 catcaaaacc gaaccgacca taccgcgcca gccgacgaaa cctactttca ttgtgttgct
     7681 ccttgaggaa aaatcgttaa taaaattatg tatggcgtaa aggaagcgtt ttaacgccgt
     7741 attgggaaaa tgtaaagttc tttatcggga tttctttaag tattggcgtt ttcggtgtcg
     7801 tggcggtaga atcggggtca gttaagaaca attatcagga acggatatgg aagaaaaaaa
     7861 cgactacacc gacgcggcat ccgacaaccg caacgggcag gaaattgaag tcggaattgc
     7921 cggcgcaggc gaccggatac ttgccgcgct gctgaaccaa ttgtttacct ttttggtttt
     7981 gttggtgccg tttgccggac tgattgcttt cgccatcaag aatgaaggca ggattggcgg
     8041 cagtgaggaa atattcgggc tgcttttggg catgacttcc ttttgggtag gactggcggg
     8101 cattttggtt tataccgtcg ttcaaatcta ctatatgagt cgggacgggc agtcattggg
     8161 taagaagatc atgagaatca gggtgttgaa aaccgacggc cgcaatccgg gttttgtcgg
     8221 cacggttttg gtacgcgaaa tcgcatggtc ggttttggtt gccattattg ccgccgttat
     8281 cggtcttgca gtaggtgaca acggagaaaa cgccatcaac ctgctggcat tccttgccaa
     8341 ctttgtcctg ctctttatgg tcaaacgcga ccgccgcacg ctttacgaca tactggcgga
     8401 tacggttgtc gtcaagctgc ccaaataaac ggacacggca aaatgccgtc tgaaagcctt
     8461 tcagacggta tttttatctt cagaaccgct caagcatcgt gcagtgaagc cgcgtgcagg
     8521 gtgttttcca tcaatgtggc aatcgtcatc ggacccacgc cgccgggaac gggcgtaatc
     8581 atcgccgccc gttcttttgc cgtttcaaat tccacgtcgc cgcacaggct gccatcgtcc
     8641 aaacggttga tgcccacatc aataacgacc gcgccaggtt tgatccattc gcctttgaca
     8701 aagttcggaa tgcctacgcc gaccaccaaa atatcggctc cggcaacctc gtctgtcaga
     8761 ttttcggttg cgctgtggca gaccgttacc gttgcgcgcg ccagcagcag ttccaaagcc
     8821 tgcgggcggc cgacgatatt cgacgcgccg accacgaccg cttttttccc cttcggatca
     8881 atgccgtaag cttccaaaag cgtcatcacg cccttgggcg tacacgggcg catcagcggc
     8941 attttgaccg ccagcctgcc gacattgtaa ggatggaagc cgtccacgtc cttatccggc
     9001 gaaatacgtt ccaaaaccgc ctggctgtcg aggtgcttcg gcagcggtag ctgaaccaga
     9061 ataccgtcca cttcggaatc ggcattcagg cggtcgacca gtgccagcag ttcttcctgc
     9121 gatgttgatt cgggcagctc gtaagacagt gatttgatgc cgcatttttg gcaggcggtt
     9181 ttcttgttgc ggacataaac cgcgctggca gggtcgcctc cgaccaaaac cacggccagg
     9241 caaggcatgt gcagattgtc ctgttggcgt tgcgccaccg cttcggcaac cgcctgcagg
     9301 cgtttttgcg aaacttcttt accattgatc agttgtgccg acatatccgt ccttttgtta
     9361 aacaggtaga aaatgctgaa aattatcgca ttttccgttt gtttttacag caggatgccg
     9421 aaaataaatt tgccctgaaa taaaatacgg ctgtttggac ggattaaata ttttttcaaa
     9481 tcaaaaaact atatttttca tcttggtgtc ttgaaaataa atattgacgc atctttccct
     9541 gagttgtata gttcgcttct tcaagtcggg gcgtagcgca gcccggttag cgcatctgct
     9601 ttgggagcag agggtcgtga gttcgaatcc caccgccccg accaatcaat cagcacgaat
     9661 cggcatgagc gcccgtagct caaccggata gagcaccgac cttctaagtc gggggttaca
     9721 ggttcgattc ctgtcgggcg tgccaagaat ttgattgaaa accgttttta tggtggctgt
     9781 agctcagttg gtagagcccc ggattgtgat tccggttgtc gtgggttcga gccccatcag
     9841 ccaccccaga taaaggcccg tacttttcgg tacgggtctt tttccgtttg cacgtactgt
     9901 ccgcaccgtc gtaccggtgc ggcggtatgc cgtctgaaag ccgggccggt catacggaaa
     9961 ccgcttcaga cggcatttgc gccgccggct ttgcctttcg catatgcttc tgtatcatgt
    10021 aggttttctg tcttttatgg tttttgtcgg agactttgat ggttgacgct tggtctgttc
    10081 ctgattttga atcgaaaatc tgtccgcccg aggcgttggc ggcgcgtttg gcgttgttgc
    10141 cgcgcccgct ggtgtttacc aacggctgtt tcgacatcct ccacaggggg cacgttactt
    10201 atctggcgca ggcgcgttcg atggggggcg cgttggtgct ggcgttgaat accgatgctt
    10261 cggtgcggcg tttgggcaag ggcggcgacc gcccgattaa tcctttggag aaccgtgccg
    10321 ccgttgccgc cgcgttggaa agtgtggatt tggtaacgtg gtttgacggg gatacgccgg
    10381 cggcgttgat tgaggcggtc aaacctgaga ttttggtcaa gggcggcgat tgggctgcgg
    10441 ataagattgt cggtgcggca gaaacgttgg cgcgcggcgg tcaggtgttt tcaattccgt
    10501 ttctgcacca gacttcgaca acgaagactt tggcaaaaat ccgtgcggca gagggcggaa
    10561 aatgacggtt ttgaagcctt cgcactggcg ggtgttggcg gagcttgccg acggtttgcc
    10621 gcaacacgtc tcgcaactgg cgcgtatggc ggatatgaag ccgcagcagc tcaacggttt
    10681 ttggcagcag atgccggcgc acatacgcgg gctgttgcgc caacacgacg gctattggcg
    10741 gctggtgcgc ccattggcgg ttttcgatgc cgaaggtttg cgcgagctgg gggaaaggtc
    10801 gggttttcag acggcattga agcacgagtg cgcgtccagc aacgacgaga tactggaatt
    10861 ggcgcggatt gcgccggaca aggcgcacaa aaccatatgt gtgacccacc tgcaaagtaa
    10921 gggcaggggg cggcaggggc ggaagtggtc gcaccgtttg ggcgagtgtc tgatgttcag
    10981 ttttggctgg gtgtttgacc ggccgcagta tgagttgggt tcgctgtcgc ctgttgcggc
    11041 agtggcgtgc cggcgcgcct tgtcgcgttt gggtttgaaa acgcaaatca agtggccaaa
    11101 cgatttggtc gtcggacgcg acaaattggg cggcattctg attgaaacgg tcaggacggg
    11161 cggcaaaacg gttgccgtgg tcggtatcgg catcaatttc gtgctgccca aggaagtgga
    11221 aaacgccgct tccgtgcaat cgctgtttca gacggcatcg cggcggggaa atgccgatgc
    11281 cgccgtgttg ctggaaacgc tgttggcgga acttgatgcg gtgttgttgc aatatgcgcg
    11341 ggacggattt gcgccttttg tggcggaata tcaggctgcc aaccgcgacc acggcaaggc
    11401 ggtattgctg ttgcgcgacg gcgaaaccgt gttcgaaggc acggttaaag gcgtggacgg
    11461 acaaggcgtt ctgcacttgg aaacggcaga gggcaaacag acggtcgtca gcggcgaaat
    11521 cagcctgcgg tccgacgaca ggccggtttc cgtgccgaag cggcgggatt cggaacgttt
    11581 tctgctgttg gacggcggca acagccggct caagtgggcg tgggtggaaa acggcacgtt
    11641 cgcaaccgtc ggtagcgcgc cgtaccgcga tttgtcgcct ttgggcgcgg agtgggcgga
    11701 aaaggtggat ggaaatgtcc gcatcgtcgg ttgcgccgtg tgcggagaat tcaaaaaggc
    11761 acaagtgcag gaacagctcg cccgaaaaat cgagtggctg ccgtcttccg cacaggcttt
    11821 gggcatacgc aaccactacc gccaccccga agaacacggt tccgaccgct ggttcaacgc
    11881 cttgggcagc cgccgcttca gccgcaacgc ctgcgtcgtc gtcagttgcg gcacggcggt
    11941 aacggttgac gcgctcaccg atgacggaca ttatctcggg ggaaccatca tgcccggttt
    12001 ccacctgatg aaagaatcgc tcgccgtccg aaccgccaac ctcaaccggc acgccggtaa
    12061 gcgttatcct ttcccgacca caacgggcaa tgccgtcgcc agcggcatga tggatgcggt
    12121 ttgcggctcg gttatgatga tgcacgggcg tttgaaagaa aaaaccgggg cgggcaagcc
    12181 tgtcgatgtc atcattaccg gcggcggcgc ggcaaaagtt gccgaagccc tgccgcctgc
    12241 atttttggcg gaaaataccg tgcgcgtggc ggacaacctc gtcattcacg ggctgctgaa
    12301 cctgattgcc gccgaaggcg gggaatcgga acatacttaa aaaatacacc ctttttcaac
    12361 acggggcggc atccgtccga atccgtccgg attctgccgc ccgacagact gccgcccagg
    12421 cgggcggggt ttcaaacatt gaggaaattc taatgaaatg gctcttcacc cttttggcgg
    12481 tactcaatat tgccgtattc ggcggcacgg tgggctataa attggccgtt aaagcgacgg
    12541 gcggcgtgcc ggaaaaccgg gccgtcgaaa acacgccccc cgcaacgccc gccgccgaca
    12601 atgcggcggc atccgtggag gatacggcgg cactgctcaa accgggcgac atcctgaccg
    12661 aagagcaggc cgagcagttg cgcttgaaaa aggaagcgga acagaaaaaa ctgagggaga
    12721 aaaaacagcg tgaagaaaaa gcccgccgcg aaaaactcgc cgcagaaaag gcgcaggcgg
    12781 aacgcgaaaa cggcgcggcg gatgccttat gcgccgcgca ggcaagcctc acgatggacg
    12841 aagatgacta ccaccgcatc aaaggacttt tgggcaaatg gtcgcacgtt gccagcagga
    12901 gcgtcgaaaa acgcaccgcc aaagccaaac ctgccgacaa aacctaccgc gtcgtcctgc
    12961 ccgtttccgc cgatgccgaa aatcaggcgg cggagctgtc tgccaaaggt ttcaacccca
    13021 taccgtttga cggcgcattg agtttgggtg tcggcaacag ccgggaaaac gcccaagccc
    13081 tgcaaaaccg gcttgccggc gccggattcg gcggggcgta tattgtcgaa cactttgccg
    13141 aagccgacag gcaggacgat tctttgagcg tgtcgcgtat gacggttttg tttaccggcg
    13201 tgaatgccgc cgatgcggac gaaatccgta aaatcacgtc cctatacggc aaactgaacc
    13261 tcaagtcttg caaataggcg cgaaagccgg acgataaacg ccaaacccac cgcgaaggtg
    13321 ggtttggcgt ttcggttttg agtatctgat atataaaaag ggaatgtatg tctcgtatat
    13381 gccgactcca agtgtgaaag tgatgatggg gaaatatggt gtttaatggt tcggaacgga
    13441 cggcttccga gtggttttga ttatacgcta ataataataa ttatcaataa aaatttaaaa
    13501 ataaatgcag attttttgta atacgatgaa aaataaacaa attaacctgt ccgccgatgt
    13561 ttgtgattcg ggtcggcaaa ctttcattgt gtcggacaaa tcggggtcgg caggcatagc
    13621 tgagggcttc gtgcctgacg gaaataaggt tgcccgtgtt tgaacatccg tccgatttgg
    13681 cgcggacagg gttgtcattc gggggtgttt gagcgtggat tcggggatgc gaaagcggta
    13741 aaaccgttgc ggatgaaggc ttacggacgg ctttaggtat ttttaagtgt attcaaacaa
    13801 caacaaaacg cttcttctat tgccgtattt agggcttgtg cggcagcctg cacaccgtgg
    13861 aggccgagtg tttcgttggt tgcggcatcg ggacgggcgg caaggttttc cggccggttt
    13921 ttccattttt accgacaaag ccgtgatgcc ggggaatggc ggtttgtccg tttcaggctg
    13981 ccgccgtttt ggaccgcgat gccgtctgaa gccttcagac ggcatttttg ctgccttttt
    14041 caatattccg ccgaatgcca aaacggttgt ccgactgtcg gcgtgctggc ttgcgctttg
    14101 tcgcgtgctt cgaccggtcc ggcttcaaat gccagccgtc cggattcgac ggcgagtgcg
    14161 aaggcgcgtg ccatattgac cggatcgccg ctgcgggaaa cggcagtatt caaaagcacg
    14221 ccgtcaaagc cccattccat cacttgtgcc gcctgtgagg gcaaacccaa gcccgcgtcg
    14281 ataatcagcg gcgtgtcggg caggcgttcg cgcaggacgt tcaacgcgta ggcgtgaacc
    14341 gcgcccaaac ccgtgccgat cggggccgcc cacggcatca acgcctgaca gcccgcgtcg
    14401 agcaggcggc ggcaggcaat caggtcttcg gtgcaataag gcagcacttt gaagccgtct
    14461 ttaatcagga tttccgccgc ttcgacaagt tggaacacat ccggctgcaa ggtgtcgtcg
    14521 tcgccgatga gttcgagttt aatccaatcg gtttcaaaca cttcgcgcgc catttgcgcc
    14581 gtcgttaccg cttcctgcac gctttggcag cctgccgtgt tcggcaggac gggaacgccg
    14641 gtttcttgaa gcagcgacca aaacccctga ccgtgcgcct cgccgccgca tcccgcgcgg
    14701 cgcagcgaga cggtaatcat cgcgggccgg gcggttcgga cggattgttt gaggatttca
    14761 ggggtcgggt aggcggctgt gccgagcagc agccgcgaag ggaaagtttc gctgtacagg
    14821 gtgagcataa tgggttcctt tgtatagtgg attaacaaaa accagtacgg cgttgcctcg
    14881 tcttagctca aagaaaacga ttctctaagg tgctgaagca ctaagtgaat cggttccgta
    14941 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt taaagttaat ccaccataac
    15001 gttgttttgg ggacagggg
//
LOCUS       DAWKQB010000037        14927 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.037, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000037 DAWKQB010000000
VERSION     DAWKQB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.037"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJL14_000863"
     CDS             complement(104..1888)
                     /gene="lpdA"
                     /locus_tag="WJL14_000863"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ7655957.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVIEAEGAAAAPKAEAPVAPAQEAPKAAA
                     PAPQAAQFGGSADVEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRGYKDGVVSRLTTGLAGMAKG
                     RKVDIIQGDGQFLDPHHLEVSLTTSEVYEQATPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIFDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMINTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1966..3573)
                     /gene="aceF"
                     /locus_tag="WJL14_000864"
     CDS             complement(1966..3573)
                     /gene="aceF"
                     /locus_tag="WJL14_000864"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ7655958.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPAA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFFKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPS
                     APAAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQ
                     GGAAKPAAVGASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPH
                     VTVHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDN
                     LVLKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQG
                     ACFTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDH
                     RVIDGAAGMRFTVFLANLLKDFRRITL"
     gene            complement(3723..6386)
                     /gene="aceE"
                     /locus_tag="WJL14_000865"
     CDS             complement(3723..6386)
                     /gene="aceE"
                     /locus_tag="WJL14_000865"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ7655959.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKTLVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRVGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7080..8792
                     /locus_tag="WJL14_000866"
     CDS             7080..8792
                     /locus_tag="WJL14_000866"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ7655960.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLEFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANVELAPTLPLKGERTAAQAELVKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMASFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRDNEAQAIAIGEIVARVTA
                     SLNA"
     gene            8856..9512
                     /locus_tag="WJL14_000867"
     CDS             8856..9512
                     /locus_tag="WJL14_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ7655961.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTSSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9522..9977)
                     /gene="ruvX"
                     /locus_tag="WJL14_000868"
     CDS             complement(9522..9977)
                     /gene="ruvX"
                     /locus_tag="WJL14_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ7655962.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLATVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9970..10518)
                     /locus_tag="WJL14_000869"
     CDS             complement(9970..10518)
                     /locus_tag="WJL14_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ7655963.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPFRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKILISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10520..11080)
                     /locus_tag="WJL14_000870"
     CDS             complement(10520..11080)
                     /locus_tag="WJL14_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ7655964.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11408..13228
                     /locus_tag="WJL14_000871"
     CDS             11408..13228
                     /locus_tag="WJL14_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ7655965.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLK
                     TEVAATKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKD
                     LEKQQKALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDA
                     RKLLEQKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKA
                     EARRAEMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGD
                     VWKGVFYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKG
                     YMVAAGSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13348..14832
                     /locus_tag="WJL14_000872"
     CDS             13348..14832
                     /locus_tag="WJL14_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ7655966.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3573 a         3669 c         4192 g         3493 t
ORIGIN      
        1 tcggagaaga tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgccgc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcagcatca aaaatcagct tggtgaaacc attgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagcagcc catgggaagt tggctttggt gattttgcgg ccggaggctt
      421 tggcagacaa ttcggtttcg cccacccatg ccacttcagg agaagtgtaa gcaacgcccg
      481 gaatgacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gcgagcatag gctgaccgac gatatcgccg atagcgtaga
      601 tgtgcggcac gttggtacgc atttgtttgt ccacttcgat gaagccgcga tcggtaacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggttctttag gtgcatttgc accttcgaag gtaacgtaaa
      781 caccgtcttc tttaggctcg acagcaacag ttttggtgtt gatcatgatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttca ccaagtcgcg gtctgcgcct tgcatcagac
      901 cgtccatcat ttcaactaca tccaggcgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgaggccgat aataccgccg ccgatgatca acagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggagtcgaaa attcgaggat cttcagggat gaaaggcagt ttggttacgc
     1081 ggctgcctgc tgcaataatg cagtttttaa aggcaacgat ttttttctca ccggtaggag
     1141 tcgcttgttc gtacacttcg ctggtcgtca aagaaacttc caagtggtgt ggatccaaga
     1201 attggccgtc accttggata atgtctactt tacggccttt agccatgcct gccaaaccgg
     1261 tagtcaggcg ggaaaccacg ccgtctttgt agccgcgcag catgtcgatg tcgagttcag
     1321 gctcagggta tttgataccg ttggcagcca agtggcgtac ttcatcgata acggcagcat
     1381 tgtgcaacag tgctttggaa gggatacagc caacgttcaa gcaaacgccg cccaaagttt
     1441 tgtaacgttc aacgatggca actttcaagc cttcatcagc agcagcaaat gcagcagaat
     1501 aaccgccggg gccgccgccc aatacgacca cgtcgtactc gacatcggca gaaccgccga
     1561 attgcgcagc ttgcggagca ggagcggctg ctttaggtgc ttcttgtgca ggagcgacag
     1621 gcgcttcggc tttaggagca gcagccgcgc cctcggcttc aatgacgaca atcaaaccgc
     1681 cttcggagat tttgtcgcct actttaactt tgacttcttt gaccacgcct gcaacttcgg
     1741 caggtacgtc catagtcgct ttatcggttt ccaaagtaat cagggtatcg tccacagcaa
     1801 tggtatcgcc cacatttact tcaaccgcaa taatatctac attttcgtga ccgccaatgt
     1861 cgggcacttt caattcaact aagctcatgt ctaatctttc tgaatgatgt tcggacttct
     1921 tttttcagac ggccttttta ttcaggccgt ctgaaaatgc tcggattaca gagtaatgcg
     1981 gcggaagtct ttcaacaggt tggccaggaa tacggtgaag cgcataccgg ccgcgccgtc
     2041 gatgacacgg tggtcaaagg acaggctcaa tgggcacatc aggcgaggag cgaattcttt
     2101 gccgttccaa accggtttga tttgggattt gcacacgccc aagatggcga cttcgggagc
     2161 gttcacaatc ggcgtgaaac ctgtgccgcc gatgccgccc aagctggaaa tggtaaagca
     2221 tgcgccttgc atttcttgtg gtttgagctt gccttcgcgg gcttttttag acaattcggt
     2281 cagctcttgg ctgatttgtt tcaagccttt ttgatccacg tctttgatta ctggaacaac
     2341 caagccgttc ggcgtgtctg ctgcgaaacc gatgttgaag tagtttttca gcaccaagtt
     2401 gtcgccgtcc agagaagcgt tgaattcggg gaaggctttc agcgcggaaa cggaggcttt
     2461 gatgatgaac gccaacggtg acagtttcac gccttcgcgt tcccattctt tgttcagttg
     2521 tttgcggaat tcttccagct cggtcatatc tgcttcttcg tgtacggtaa cgtggggaat
     2581 caccacccag ttgcgagaca ggttttgacc ggaaattttc ttaatgcggg acaattcttt
     2641 aacttcgaca ttaccgaatt tggagaagtc cactttaggc cagggcagca agtccagacc
     2701 gccgcctaaa gacgcgccga ctgcggcagg ttttgccgcg ccgccctgca ttacggattt
     2761 cacaaaggct ttgatgtcgt cgcccacgat gcggcctttc aagccggtac ctttgacttg
     2821 gcccaaatcc acgcccaatt cgcgcgccag tttgcgtgcg gaagggccgg cgtgtgcttt
     2881 ggcgaaagcg gcctcgtcga ttttggcagc ggcaggtgca gaaggtgcag gcgcggcggc
     2941 gggtgcggcg gcagcagtcg gaggcggagc ggcagccggt gcggcagctt gagcggcttg
     3001 agcaggggct gctgcggcag agccgacggt ttctacttcg ataatggcag agccttcgga
     3061 tactttgtcg ccgactttga agaatacggc tttaacgaca ccggcagcgg tacaaggtac
     3121 gtccattgtc gctttatcgg tttccaaagt aatcagcgtg tcgtcttcgg caacggtgtc
     3181 gcccactttg atttcaacgg cgattacatc cacatcggta tggccgccga tatcgggaac
     3241 ggctacttga acggttgcac cgcctgcggc agcgggtgcg gcagcaggtg caggttgtgc
     3301 ttcggcagca gccggggcgg cttcggcggc ggcggcaccg gtttcaacgg tcagaattac
     3361 gccgccttcg gagattttgt cgccgacttt gactttcact tctttcacga caccggccgc
     3421 atcggcaggc acatccatcg tggctttgtc ggtttccagt gtaatcaggg tgtcgtcaac
     3481 ggcgatggtg tcgcccgctt taacttctac ggcgatgatg tcgacgtttt cgtgaccgcc
     3541 gatatcgggg actttgattt ctacgatact catttgagtt cctttaatct tgttcaattg
     3601 gatgccgtct gaaagctgct tcaggcggca tcggtacggt ttgtctgctc aaaccattcg
     3661 ggcattcggt tttcagacgg cctgatgtcg gaatggggca aacaggccgt ctgaaacatc
     3721 aatcagcgtt tccagctagg agctgaatcg gctttgatgc cgtatttctc aatggcttgt
     3781 tgaacggttt ctttgctgac tttgccttgt tccgccaatg cggccaatgc ggccacggca
     3841 acgttgtagc gatccacttc aaagaagcgg cgcaggttgg cgcgactgtc ggaacggccg
     3901 aaaccgtcag tgcccaagac gtggtagtcg ttcgggatgt aggcgcggat acggtcagca
     3961 tagctgcgga tatagtcggt agcggcaatc accggaccgt catgaccttg cagttgggaa
     4021 gtaacgaaag gtactttttc ggcttccagc ggatgcaggc ggttgaagcg ttctacctcg
     4081 acagcgtcgc ggtgcagcag gttgaaggac gggcaagacc agatgtctgc ttctacgccg
     4141 aagtcggctt tcagcagctc ggcaccggca atgacttctt gcaggatggt accggagccc
     4201 atcaattgaa ctttcttgtc gcctttgccg ccggctttca gcagatacat acctttcagg
     4261 atgtcttgtt ccgcaccttc gggcatatcc ggatgggtgt agttctcgtt catcagggtg
     4321 atgtagtaga acacgtcttc attattggca tacatacggc gcagaccgtc ttgtacgatg
     4381 acggcgactt cgtattggaa agtcgggtca taagatacgc agttcggaat caggtcggcc
     4441 tggatgtggc tgtggccgtc ttcgtgttgc aggccttcgc cgttcagcgt cgtacggccg
     4501 gcagtaccgc ccagcaggaa gccgcgcgcg tgcatatcac cggctgccca tgccaagtcg
     4561 ccgacacgtt ggaaaccgaa catagaatag taaatgtaga acggaatcat cgcgaagttg
     4621 ctgttggcgt agctggttgc agccgcaatc cagtcggcca tcgcgcccgg ttcgttaata
     4681 ccttcttgca agatttgacc gtcaacggat tctttataga acatcagttg gtctttgtct
     4741 tgaggggtat attgctgacc tttcggattc caaataccgt attggcggaa cataccttcc
     4801 atgccgaaag tacggctttc gtcgggaacg ataggtacga cgcgtttgcc gatttttttg
     4861 tctttcagca aagtggacag gatgcggacg aatgccatcg tggtcgagaa ctcgcgttca
     4921 ccgctggatt tgagttgtgc gtcgaatgct gacagctcgg gcacttccaa tacttcctgc
     4981 gtcggtttgc gttgcggcag gtagccaccc aaagcatcgc ggcgtgcgtg caggtatttg
     5041 tattcttccg tatcgggggc aaaagtcagg taaggcagat cgccgctttc gatttgttcg
     5101 tcggtaaccg gaatgtcaaa gcggtcgcgg aattgtttca gggacgcttt gtccattttt
     5161 ttggcttggt gggcaacgtt ctgaccttcg ccggatgcgc ccataccgta acctttaatg
     5221 gttttcgcca agatgacggt aggtttgccg tcggcatggt tcgctgcgcg gtcgtaggcg
     5281 ttgtacactt tttgcgggtc gtggccgccg cggttcaatg cccagagttg ctcatcggtc
     5341 atatcggcaa ccaatgtttt cagttcgggc gtattgaaga agtgttcgcg cacatacgcg
     5401 ccgtctttgg atttgtaagt ttggtagtcg ccgtccaaac attcttccat acgttggcgc
     5461 aggataccgt ctttgtcttt cgccaagagg cggtcccagc ggcggcccca aatgactttg
     5521 acgacattcc agccggcgcc ggcaaagttg ccttccaatt cttggatgat tttgccgttg
     5581 ccgcgtaccg gaccgtccaa gcgttgcagg ttgcagttga tgacgaaaat caggttgtcc
     5641 aagccttcgc gtgcagccag tgcgattgca ccttgagatt cgggttcgtc catttcgccg
     5701 tcgccgcaga aacaccatac tttacggcct ttggtttttg ccaaaccacg cgattccaag
     5761 tatttcagga aacgcgcctg ataaatcgcc atgatgggcc ccaagcccat ggatacggtc
     5821 gggaactgcc aaaagtcggg caagaggtgg gggtgcggat aggaaggcag accgtgtccg
     5881 tccacttctt ggcggaagtt gttcagctgg tcttcggtca gacggccctc gacgaatgcg
     5941 cgtgcataga tgcccggggc gacgtgacct tggaagaaga ccaaatcgcc ttcttcgcct
     6001 tcgcctttgg ctttccaaaa gtggttgaaa ccgacttcgt acatggtggc ggcagattgg
     6061 aaagatgcga tgtgcccacc cagttccaaa tctttcttgc cggcgcgcaa tacgatggcg
     6121 gcggcgttcc agcgcacgaa tgcgcggatg cggtgttcga tgttttggtc gcccggaatg
     6181 cctttttcgt tttcaaccga aacggtattc aaatacgggg tggtcgtgcc gtgtggcata
     6241 cgtacgccct tgtcgcggca gtatttgacc aggttttcca agaggtattg cgcgcgttcg
     6301 ccgccttcat attcgaggac ggagcttaac gcgtccagcc actcttgggt ttcgataggg
     6361 tcaacatcgt gtaattgggt ggacatagta tctatccttt atgttgagtg tttaatcggc
     6421 agggagggtg tagccaaccc ctttattttc gtttgtgaaa aataataagc aaaaacgaaa
     6481 atttctaaat caaaccgaaa aaaataattt aagtttaatg ttttcaaaaa attataaaag
     6541 ttttggaaac aagggaaaac ttgaaaaaaa tgttcgatct gctccacacg aaatcctatc
     6601 agcaatcggg tctgctggca aataaccatg cctgtaaaag caggttttat actgaagttg
     6661 gcgtttggta aggtcaagtc tttttgattt cgaaatattc tgtttgattt tggcgatatt
     6721 ttttcggaat tgtggtatat cgaatcatta tgtctctgtc ggcaattttc ctattgtaga
     6781 aaatctatac acaagctttg tggataaccg tgttgataag ccacggcttg tgttaaaaaa
     6841 gtcttacgca acaaatgtac gcttattttg attaaaaaat aggcacattt aaatctataa
     6901 aaatcatgta cttatgttca atatgatgtt tctggtcatg tttttgccgt ctgttatggg
     6961 atttgtcccg cgcttggggc agggggtgaa ttttggaggt tggtgcaaaa cctgcttttt
     7021 tcgcgttaca atctgcaccc gatttacttt tgaatttgcc tgtttggaaa aatactttta
     7081 tgaaagccag ccaattcttt atctctactt taaaagaagc ccctgccgaa gccgcgcttg
     7141 ccagccacaa gctgatgatt cgcgccggcc tgattaaagc caacgcgtcc ggcctttata
     7201 cttggatgcc gatggggctg cgcgtgttac gcaaagtcga aaacgtcgtg cgcgaggaaa
     7261 tggcgcgcgc gggcagcgtg gagctgctga tgccggtggt gcagcctgcc gagctgtggc
     7321 aggaatccgg ccgctgggaa ttttacggta aagaactgct gcgcctgaaa gaccgccacg
     7381 accgcgattt ctgcatggga ccgacctgcg aggaagtcat cgccgacatc gtgcgtaaag
     7441 agatcaacag ctacaaacaa ctgccgaaaa acttttacca catccaaacc aaattccgtg
     7501 acgaagtgcg cccgcgcttc ggtgtgatgc gtgcgcgcga gtttgtcatg aaagatgctt
     7561 attccttcca tgccgactac gcctcgcttc agacgaccta tgatgccatg tatgacgctt
     7621 actgccgtat cttcacccgt ctgggcttgg aattccgccc cgttgccgca gataccggca
     7681 gcatcggcgg taccggctct cacgagtttc aagtgttggc ggaaagcggc gaagatgtga
     7741 ttgcatacag cgacacttcc gattacgccg ccaatgtcga gctggcaccg accttgccgc
     7801 ttaaaggcga acgcaccgct gctcaggcag aactcgtcaa agtacacacg ccaaacgtca
     7861 aaaccattga gtctttggtt gaattcctga atattccggt tgaacaaacc ctcaaatcca
     7921 tcgtggttga aggcgaaaat gaaggcgaaa tcgttttatt gctgttgcgc ggcgaccatg
     7981 agttcaacga catcaaagcc gaaaaactcg caggtgtgaa atcgccattg acgatggcaa
     8041 gccctgccgc gattgtcgaa cagttcggcg caaacggcgg ctcgctgggt ccggtcggct
     8101 ttgcaggtaa agtttatgcc gatttcgcca ctgaaaaagg cgcggactgg gtcatcggtg
     8161 cgaacgaaga cggctaccac tataccggct tcaacttcgg gcgcgatgcc gccgagcctg
     8221 aattcgtcga tttgcgtaat gtggtcgaag gcgacgaaag ccccgacggg caaggccgtc
     8281 tgaaactggc gcgcggcatc gaagtcggac acgtcttcca gttgcgcgac aaatacaccc
     8341 aagccatgaa cgtgagcttc ctcgacaaca acggcaaatc gcaaatcatg gaaatgggct
     8401 gctacggtat cggcatcacc cgcgtcgttg ccgccgccat cgagcagaac aacgacgaaa
     8461 aaggcatcat ctggacgaag gcgatggcgt cgtttgaagt cgttatcgtg ccgatgaact
     8521 acaaaaaatc agacaccgta cgcgaagccg ccgacaaaat ctatgccgaa ttgctggcgg
     8581 caggcgcgga tgtgctgctg gatgaccgcg acgaacgcgc aggcgtattg ctgaacgatt
     8641 ccgagctgtt gggtattccg caccgcatcg tcatcggcga ccgcgccttg aaagaaggca
     8701 atgtcgaata cgccgaacgc cgcgacaacg aagcgcaggc aattgcaatc ggagaaattg
     8761 ttgcacgtgt aacagcttca ttaaatgcgt aagcttggtc gaatgccgtc tgaaaataat
     8821 ttcagacggc atttttactt aggggggaaa gtgaagtggc ttcagtattt ttagaaggag
     8881 aggcagtcga ggtcggcaat attttctgta tcggcaggaa ctatgccgca catatcgaag
     8941 agttaaaaaa cgaaatacct tccgaacctg tggtgtttat gaagccgtca ggcagcattt
     9001 tgaacagcgg cggtacaatc ttgctgcccg agtttagtag ggatgtgcag tttgagtgcg
     9061 aactcgtgct gctggtcggt aaggattcag acggcacggg cgagggtaaa gatattttgg
     9121 gatgtgtcgc aggatatggt gtagggctgg accttacggc acgggatatc caatgccgtc
     9181 tgaaggaaaa agggctgccc tggctgaagg caaaagggtt caggcattcc gcgtgcgtgt
     9241 cggattttgc ggcggcaggc agaatcggca atcccgagaa tgtgttgttt tcattaaaac
     9301 aaaacggcgt attgaaacag cgcggggata ccggtttgat gatttacccg attcgggaaa
     9361 ttttacacaa actggcggca gattacggtt tggggaaagg ggatttggtg tttaccggaa
     9421 cgtcctccgg cgtgggcgca atcggtgcgg gggacaattt ggcattggag ctggacggct
     9481 tggtgcgcgc ctctttcacg gtagggtgtt aaccgcgccg cttaaccttc acgcccgttg
     9541 aaacattccg ccggaccgcc ctcgaaaaaa ccgtgcaaga tggcttgcgc cgccacttgg
     9601 tcgagcaccg atttgcgttt tttgccgaag acctgtgctt ccgaaagcag gctttcggca
     9661 tagacggacg acagccgttc gtcaacccaa tagacgggga gattgaacct gccgttcagc
     9721 ctgcgtccga acttgcgcga caggtgcgtc atttcatgtt tcgtgccgtc ggtatgcacg
     9781 ggcagtccga cgacaaaata acgcggctgc cattcttgaa ccagcttggc gattgccgcg
     9841 aacttttcat cgttgctgcc gccggtaacg gtggccagcg gatgggacag ccccaattcc
     9901 gcatcgccct gcgccacgcc gatgcgcgct tcgccgaagt cgaatgccag tgccgttcct
     9961 tttggaattt tatgcatggc cggctcccga aaacagggcg agcgggtcga tgccgagttt
    10021 ggcgaatgcg gcggcgtaac ggtgttcgta ggggatgtcg aacaggatat gttcgtcggc
    10081 gggaacggtc agccacgcat tgtcggcaag ttcgcgttcg agctggcctt ttccccagct
    10141 tgaatagccg atactgatta atattttgtc aaccgcgcct tcgcgtgaaa tattttcaat
    10201 cacgtctcgg gaagaagtca gcgcgatatt gtccgaaacg ccgatactgc tttgccagtt
    10261 gccgatcggg gtatgcacga cataacctcg ctcgacttgc accggtccgc ccatcatcac
    10321 gctgtcgtgc tgcattcgga aagggatgtt tttgccggtg gcggaaaaaa tcatgtccat
    10381 cgtaatcgga gagggtttgt tgatggcgat gccgagtgcg ccgtcttcat cgtgtttgca
    10441 gatatagacg accgattgtg aaaaaaacgc gtcttccata tcgggcatgg cgacgagaaa
    10501 gtggttggag aggttcatat cagcgtttgt cgggattttc tatcgggttg gaaatcatgc
    10561 aggcgccggc aagcaggttg ccggaagtat cggcttgcac cccatcggtt tgcggtatgc
    10621 cgccgccgaa acaggaaacc gcgcttaagg aatttttcgg cgagccttgg atgattttat
    10681 cgctgtacac caaataggcg aaggttttgc gtttggggtc gtaataacgg acaatctgcc
    10741 ggctcttgaa cgcgaagctc gcaccgtgtt tgaaaacttc tttcggtttg cgcacggcgg
    10801 tttcgtcaaa agaaatcgaa gatgccgtct gaacgcacga aaccgatgcg tcggacgcgt
    10861 cctcttccaa attgaccatt tccttcaagc cgcctttttt tgcatacgaa atataacagg
    10921 caaccccttg aacgtcggga tcgtcgaatc cttccacttc gatacggtcg tttttgccca
    10981 gtatgttgaa aacggtactt gcccgtccga ttttatcggt ttcgccgctg ccgcaggcag
    11041 tcagcaggac ggcggcagac agcagtagca gtctgttcat caaaatttcc ttatcggttg
    11101 aatccccgtt ttccgtgaag gcggcagggc ttgctttgtt tggaggggcg cacgttccca
    11161 aaatcgggca acatacggta tgcggcttaa tgtgctaccc tgtgtgtcgg aacacaatat
    11221 ccgttgcggc attgaaaata tgacgcacaa tataaggctg tttgagccga ttgcaagtat
    11281 ccgcgcaaag aaggcgcaag attgggcaaa cggcattttc agatggcata actgacagta
    11341 taatccaaac gtccggctgc tttcgtgcag ccttcagtat tatccaactt tttccgaagg
    11401 tcgaaccatg cgctacaaac cccttctgct tgccctgatg ctcgtttttt ccacgcccgc
    11461 cgttgccgcc cacgacgcgg cacacaaccg ttccgccgaa gtgaaaaaac agacgaagaa
    11521 caaaaaagaa cagcccgaag cggcggaagg caaaaaagaa aaaggcaaaa atggcgcagt
    11581 gaaagataaa aaaacaggcg gcaaagaggc ggcaaaagag ggcaaagagt ccaaaaaaac
    11641 cgccaaaaac cgcaaagaag cagagaagga ggcgacatcc aggcagtctg cgcgcaaagg
    11701 acgcgaaggg gataagaaat cgaaggcgga acacaaaaag gcacatggca agcccgtgtc
    11761 cggatccaaa gaaaaaaacg caaaaacaca gcctgaaaac aagcaaggca aaaaagaggc
    11821 aaaaggacag ggcaatccgc gcaagggcgg caaggcggaa aaagacactg tttctgcaaa
    11881 taaaaaagtc cgttccgaca agaacggcaa agcagtgaaa caggataaaa aatacaggga
    11941 agagaaaaat gccaaaaccg attccgacga attgaaagcc gccgttgccg ctgccaccaa
    12001 tgatgtcgaa aacaaaaaag ccttgctcaa acaaagcgaa ggaatgctgc ttcatgtcag
    12061 caattccctc aagcagcttc aggaagagcg tatccgccaa gaacgtatcc gccaagagcg
    12121 tatccgccaa gagcgtatcc gtcaggcgcg cggcaacctt gcttccgtca accgcaaaca
    12181 gcgcgaggct tgggacaagt tccaaaaact caataccgag ctgaaccgtt tgaaaacgga
    12241 agtcgccgct acgaaagcgc agatttcccg tttcgtatcg gggaactata aaaacagcca
    12301 gccgaatgcg gttgccctgt tcctgaaaaa cgccgaaccg ggtcagaaaa accgcttttt
    12361 gcgttatacg cgttatgtaa acgcctccaa tcgggaagtt gtcaaggatt tggaaaaaca
    12421 gcagaaggct ttggcggtac aagagcagaa aatcaacaat gagcttgccc gtttgaagaa
    12481 aattcaggca aacgtgcaat ccctgctgaa aaaacagggt gtaaccgatg cggcggaaca
    12541 gacggaaagc cgcagacaga atgccaaaat cgccaaagat gcccgaaaac tgctggaaca
    12601 gaaagggaac gagcagcagc tgaacaagct cttgagcaat ttggagaaga aaaaggccga
    12661 acaccgcatt caggatgcgg aagcaaaaag aaaattggct gaagccagac tggcggcagc
    12721 cgaaaaagcc agaaaagaag cggcgcagca gaaggctgaa gcacgacgtg cggaaatgtc
    12781 caacctgacc gccgaagaca ggaacatcca agcgccttcg gttatgggta tcggcagtgc
    12841 cgacggtttc agccgcatgc aaggacgttt gaaaaaaccg gttgacggtg tgccgaccgg
    12901 acttttcggg cagaaccgga gcggcggcga tgtttggaaa ggcgtgttct attccactgc
    12961 accggcaacg gttgaaagca ttgcgccggg aacggtaagc tatgcggacg agttggacgg
    13021 ctacggcaaa gtggtcgtgg tcgatcacgg cgagaactac atcagcatct atgccggttt
    13081 gagcgaaatt tccgtcggca agggttatat ggtcgcggca ggaagcaaaa tcggctcgag
    13141 cgggtcgctg ccggacgggg aagaggggct ttacctgcaa atacgttatc aaggtcaggt
    13201 attgaaccct tcgagctgga tacgttgatc ggattgccga aacggtaggc tttatgcaaa
    13261 tcgtgccgat atggtgcgct gttccgtgaa gtttgccgta cctgtatttt ccggtttttg
    13321 gaaatcaaga gagaaagtta aagaagaatg tcgaaacctg tttttaagaa aatcgcactt
    13381 tatactttgg gtgcaatcag cggcgtggcc gtcagtctgg cggtgcaggg ttttgccgcc
    13441 gagaaggaca ggcgggataa cgaagtcctg ccggtgcaat ccatccgcac aatggcggag
    13501 gtttacggtc aaatcaaggc aaactactat caggacaaac ccgatgccga tttgtttgaa
    13561 ggtgcgatga agggtatggt ggccggtttg gatccgcatt ccgaatatat ggataaaaaa
    13621 ggttatgccg agataaagga gtccaccagc ggcgaatttg gcggcttggg gatggaaatc
    13681 gggcaggaag acggatttgt caaagtggtt tcgccgattg aggacacgcc tgcggaacgg
    13741 gcgggggtga aaagcggcga tttcattgtg aaaatcgata atgtttcgac acgcggcatg
    13801 acggtcagcg aagcggtgaa gaaaatgcgg ggcaagccgg gtacgaagat tactttgacg
    13861 ctgtcgcgca aaaatgccga caagccgata gtcgtcaacc tgacccgtgc cattattaaa
    13921 gtgaaaagcg tccgccatca cctgatcgaa cccgattacg gctatatccg cgtgtcgcag
    13981 ttccaagagc ggacggtcga aagcgtcaat accgccgcaa aagagctggt aaaggaaaat
    14041 aaaggaaaac cgctcaaggg gctggtgttg gatttgcgcg acgaccccgg cgggcttttg
    14101 actggcgcgg tcggcgtgtc ggcggcattt ctgccgtctg aagcagtcgt cgtcagcacc
    14161 aagggacgcg acggcaaaga ccgcatggta ctgaaagccg ttcctgaaga ttatgtgtac
    14221 gggatgggcg gcgattcgtt ggcgggcatt cctgccgagt tgaaaaccat accgatgacg
    14281 gtattggtca attccggttc ggcttccgcg tcggagattg tcgcaggtgc attgcaggat
    14341 cataaacgcg cggtcatcgt cggtacgcag agcttcggca aaggttcggt tcagactttg
    14401 attcctttgt ccaacggcag cgcggtcaag ctgacaacgg cactgtatta tacgccgaac
    14461 gaccgttcta ttcaggcgca ggggattgtt cccgatgtcg aagtaaaaga taaggaacgc
    14521 atttttgaaa gccgcgaggc ggatttggtc ggacacatcg gcaatccttt gggcggcgag
    14581 gatgtgaaca gtgaaaccct tgccgtgccg cttgaaaaag atgcggataa gcccgctgta
    14641 aaagaaaaag gtaaaaagaa aaaggacgag gatttgtctt caaggcggat tcccaaccct
    14701 gccaaagacg accagttgcg gaaagctttg gatttagtca agtcgcccga gcagtggcag
    14761 aagtctttgg ggctggcggc gaaaaagccg gtttcaaata aagataagaa agataaaaaa
    14821 gataagaagt aggttttgac gatgccgtct gaaaggattt cagacggcat attatgtttg
    14881 aaaggacagg ttatgagcga ttttttaccg gaatttgaac cgtgcgg
//
LOCUS       DAWKQB010000038        14873 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.038, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000038 DAWKQB010000000
VERSION     DAWKQB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.038"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(90..3575)
                     /gene="smc"
                     /locus_tag="WJL14_000873"
     CDS             complement(90..3575)
                     /gene="smc"
                     /locus_tag="WJL14_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ7655967.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETATQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQAEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKDRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTTSSRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQASDHTAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLNYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSADEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIAILQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTFDWQERQQELALAYETEFQNDEQHIKLEELS
                     EAVQTLDEEYIVVQEKLAQIQEQGREQYAKVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALTQESPKVLNSSIGSLTQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQTAITLLEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3577..3828
                     /locus_tag="WJL14_000874"
     CDS             3577..3828
                     /locus_tag="WJL14_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655968.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3825..4547
                     /gene="lpxH"
                     /locus_tag="WJL14_000875"
     CDS             3825..4547
                     /gene="lpxH"
                     /locus_tag="WJL14_000875"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ7655969.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKY"
     gene            4816..6402
                     /locus_tag="WJL14_000876"
     CDS             4816..6402
                     /locus_tag="WJL14_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7655970.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYAVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6571..6774)
                     /locus_tag="WJL14_000877"
     CDS             complement(6571..6774)
                     /locus_tag="WJL14_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ7655971.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6890..7387
                     /locus_tag="WJL14_000878"
     CDS             6890..7387
                     /locus_tag="WJL14_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HEZ7655972.1"
                     /translation="MTALTLPEDIRQQEPSVLLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKITPKIIDKAKSL
                     YEKHGETIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7469..8662
                     /locus_tag="WJL14_000879"
     CDS             7469..8662
                     /locus_tag="WJL14_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ7655973.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9066..10001)
                     /gene="hemC"
                     /locus_tag="WJL14_000880"
     CDS             complement(9066..10001)
                     /gene="hemC"
                     /locus_tag="WJL14_000880"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ7655974.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10120..10635
                     /gene="ybeY"
                     /locus_tag="WJL14_000881"
     CDS             10120..10635
                     /gene="ybeY"
                     /locus_tag="WJL14_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ7655975.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARDYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPEQHFAHLTIHGTLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            10637..11461
                     /locus_tag="WJL14_000882"
     CDS             10637..11461
                     /locus_tag="WJL14_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ7655976.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11590..12969)
                     /gene="nhaC"
                     /locus_tag="WJL14_000883"
     CDS             complement(11590..12969)
                     /gene="nhaC"
                     /locus_tag="WJL14_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ7655977.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13260..14483)
                     /locus_tag="WJL14_000884"
     CDS             complement(13260..14483)
                     /locus_tag="WJL14_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ7655978.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3509 a         4186 c         3826 g         3352 t
ORIGIN      
        1 cgatttaaat ttaattcact ataccgtctg aaaacctgta tcgcaacacg tcattaagaa
       61 cggcaagatt caaccgttct gcaacccgtt caaacggctt ccgccatttc caacgcttgt
      121 ttgatgtcca cggcgacgac gcgcgatacg cctttttcct gcatcgttac gccgacgagc
      181 tgctctgcca tttccatcgt caggcggttg tgggagatgt agaggaactg ggtttgcgcc
      241 gacatttctt tgaccaggtt gcagaagcgc gaggtgttgg cgtcgtccag cggggcatcg
      301 acttcgtcca aaaggcagaa cggagcgggg ttgaggctga acagagcgaa cacgaggctc
      361 atggcggtga gggctttttc gccgccggag aggaggtgga tggtgctgtt tttcttgccg
      421 ggcggacgcg ccataatgga cacaccggcg gtcagtaggt cgtcgcctat cattttgaga
      481 gtggcttcgc cgccgccgaa cagggtcggg aagaaggttt ggactttgcc gttgacggcg
      541 tcgaaggttt ctttgaaacg cgctttggtt ttgtcgtcga tttgggcgat ggcttcttcc
      601 aaaagggtga tggcggtctg cacgtcttcg ctttggctgc ggtagtagcc gtcgcgttcg
      661 cgcgcttctt cgagttcttg cagggcggcg aggttgacgg cgccgagggc ttcgatttgc
      721 tgggtgaggc tgcctatgct gctgttcaat actttcggcg attcttgcgt caacgcttcg
      781 agcgcgtcca aatcggcggc acgttcggtc aggttttgat ggtagcgttt ggcgttaatc
      841 agggcttcct gctgctgcaa cagggcggtt tgggtggctg cctgaagctg tggcaactgg
      901 gtttgcaggg tttgtacttt agcgtattgt tccctgccct gttcctgaat ctgcgcgagt
      961 ttctcttgca caacaatata ttcttcgtcc aaggtctgta cggcttcgct taattcttca
     1021 agcttgatgt gctgctcgtc gttttggaac tcggtttcat aggcgagggc aagctcttgt
     1081 tggcgttcct gccaatcaaa ggtttgctgt tcgagctggg caatttgctg ccggtagttt
     1141 tgtttttgct ggttgagctt gtgaacggcg acttcggcca gcccgtattg gcggttggct
     1201 tccaacaggg caagctgcgc ctgtttcaga cggccttgct gctcttggcg gctgtgcgcg
     1261 gtggtttgct gctggtgttc gagttcggcg gcggcttcct gcaaaatggc aatgtcgtct
     1321 gaaagcccgt cggacgtgtg ttgcaacacg gtctgctctt ccgccaactg cgccagttcg
     1381 cgctcgatgt gttcgcggcg gatttgccct tggttggtgc gcgccaagag ttcggcggcg
     1441 cgttgctgcg cttggctgta ttggcgcgtg tgctgctgtt gctgctgcat caggttttta
     1501 tgttgcactt cggacgagcg cacggcggct tctgcctgtt tgaacgcggc ttcgtcagcg
     1561 gagagttcgg gggctaggtt gtccagttcg gatgcgatgc cgtcgaggcg cgctttttgg
     1621 gcaatcagac tttcctgcgc gggcttggca tagagcagga cgctgacttt atcgacctga
     1681 tggccttcgg gcgtgagcca gatttggtgt gcgcccaaat cgctttgatg cgcgagggcg
     1741 taattcaaat cgggcgcgca caatacgccg tcgagccagt ggtgcaatgc cgtctgaaac
     1801 ggcggctgcg cttggatttg gttcagcaat gcctgtacgg gcagggattt tttgatgccg
     1861 ccggagaggc cgtctgaaag ccacgccgcc tgcccctgcg gcaaaggttc ggggggaacg
     1921 aaaccttgcg gcacggcgcg ggcgtgcagg cgttcggcaa ggatgacgga caaggcgtgc
     1981 tgccactcgg cgggcgcggt gatgtgttgc cacagttgcg gcgcggcggt gtggtcgctt
     2041 gcctgccaga agtcggcggc ttcctgctgt tgcgacagga tttgcgacaa cgcctgctgc
     2101 tgcgcctgca aggtgatgtg ttgctgtttc aggctttgga agcggctcga ggtcgtctga
     2161 aacgcctcgc gggcggcgtg caaggcttct tcggcggcaa tgatttgttc ttcgtaatgc
     2221 tcttgccggc tttgcagcaa ggcggcggct tcctgcgcgg cagcggtttc ggcttcgtcg
     2281 ggcaggttca gggcttggtt ttcctgtttc agacgatctt tgcgctcttc gtgcttggca
     2341 acggtttgtt cggcatgggc aagctgctgc tgcttcaacg ccagttcgcg gcggatgcgg
     2401 tttgcctcgt cctgctgggt ttggaaggcg gcgttgagcg tggcttgggc ttcttccaat
     2461 tcgggcagac gctcttcgtg ttcggcaacc tgcatcgccc actccgccaa ctcggtttgc
     2521 ttttcttcgg cctgcaactc gttttcttca agctgcacgc gaatttgctg ctgctcctga
     2581 tggatgcgct gcatctgcgc ctgtgccgcc tgcttgtcgc gttcgatgcg ttggtgcagg
     2641 ttttgctgat ggcggatttg ttcttccaaa cgggcaatct gctcgcgcaa cacgccgcgt
     2701 ttgttgctca attcgtgtac cgcctgctgt tgcgactgtt cggcggtctg caaggcgtgt
     2761 acttcatcgt ttaacgcctg aacctgagtg gcggtttcgt cctgctgcgc ctgcaaagac
     2821 tgatgctgcg cggtcgcttt gtcggcggcg gcaagcgatt gctgccattg ggcgtaatcg
     2881 agcaaatcct gctggcgatt caactgcgca gtcagggatt tgtagcgttc ggcggtttcc
     2941 gcctgtttct ccagcttttc cacctgacgc gccaactcgt tctgcaaatc acccaaacgc
     3001 tgcaaatgct cgcgcgtgtc tttcagacgg ccttccgtct ccttgcggcg ttccttatat
     3061 ttggacacgc ctgccgcctc ctcgatatag gcgcgcaact cctccggccg cgcttcgatg
     3121 atgcgcgaaa tcatcccctg ctcgataacg gcataaccgc gcgcgcccac gcccgtaccc
     3181 aaaaacaaat cggtaatatc gcggcggcgc acggtctgat tgttgatgaa ataagtcgat
     3241 tcgccctgcc gcgtcagctg ccgcttgatg ctcacctcgg catactgccc ccacgcgccc
     3301 tgcaaactgt ggtcgctgtt gtcaaacacc agctccaccg aagctcttgg cgcaggacgg
     3361 cgcgttgccg caccgttaaa aatcacgtcc tgcatactct cgccgcgaag ctgcttcgcc
     3421 gaagcctcgc ccaacaccca gcgcaccgcg tcaatcacat tcgacttgcc gcagccgttg
     3481 ggcccgataa ccgcgacaag ctgccccggc acatgaattg tggtcgggtc ggtaaaagat
     3541 ttgaagccgg agagtttgat atgggtcagg cgcataatgg tcggaaaaaa taaaaaagaa
     3601 gcgtatttta acggaaatcc cgccgcacca ccccatatct tgccggcaaa accttaccgc
     3661 atcccgcacc ctcgatgccg tctgaagcct tcagacggca tttttaacgc gcccgaaccc
     3721 cgattccgcc aacgcccgat gcttggcgcg gcatttgccg tattgccccg ccgccgggcg
     3781 cgttataatc cgccacgaca tccgcccgat ttggaaacct gcctatgaaa cccgcctatt
     3841 tcatctccga cctgcatttg agcgaaaagc accccgaact gaccgcgctg ttgctgcgtt
     3901 ttttacgttc ttccgccgcc gggcaggcgc gggcggttta cattttgggc gatttgtttg
     3961 atttttgggt gggcgatgac gaagtttccg agttgaatac ttcggttgcg cgtgaaatca
     4021 ggaaattgtc cgacaaaggc gttgccgtgt tcttcgtcag gggcaaccgc gacttcctga
     4081 tcggtcagga tttttgccgg caggcgggca tgacgctgct gccggattac tcggttttgg
     4141 acttgttcgg ctgcaaaacc ctcatctgcc acggcgacac tctgtgtaca gatgacaggg
     4201 cataccaacg tttccgcaaa atcgtgcatc ggaagcggct gcaaaaactg ttcctgatgc
     4261 tgcccctgaa gtggcgcacg cgccttgccg ccaaaatcag gcgtgtcagc aaaatggaaa
     4321 aacaggtcaa gcccgccgat attatggatg tcaatgccgc ctttaccgcg cggcaggttc
     4381 gcgcctttaa cgcggaaagg ctgatacacg ggcataccca ccgcgagcat atccatcacg
     4441 aaaacggctt tacccgcatc gttttgggcg actggcataa cgactatgct tcaatcctcc
     4501 gcgtagacgg tgacggcgcg gtattcgtgc cgctggaaaa atactgaaaa tgccgtctca
     4561 agcctttcgg gcggcttttt ttacacgccc ctttcccaca ctccctccgc cgcatcatca
     4621 ccatccttaa cccgcattaa ggggcgttat cgataaaatg cttgaccgat tacataagat
     4681 tacgtaaagt gtgcaaacgc ataacaattg gtcttaccaa tttatctttt aaccaaaata
     4741 tcgggtgtac gggatttgaa cgtctttcca aacctcgacg cgccttatga tgcaaatgcc
     4801 taaaaaggat tcgttatggc acttttcctc agcatattcc ccatcgtcct gctgatttgg
     4861 ctgatggtga aaaaaaacag tatgccctcc tacgtcgcgc tgccgattac cgcagtgctg
     4921 atttacgcca tcaaactttt ctacttcgac gatgcgggca tgctgctcaa cgccaccgcc
     4981 gcttccggcc tcgtcaaaac gctcacgccg attaccgtga ttttcggcgc gattatgttc
     5041 aaccgtatga tggaaaccac gggctgcatc gatgtcatcc gcaaatggct ggcgaccatc
     5101 agccccaacc ccgtagcgca actgatgatt atcggctggg cttttgcctt tatgattgaa
     5161 ggcgcatccg gcttcggcac gcctgccgcg attgccgcgc cgattctgat gagcttgggc
     5221 ttcaacccgt tgaaagtggc gattttcact ttggtgatga actccgtccc cgtctctttc
     5281 ggcgcggtcg gtacgccgac ctggttcggt ttcgcaccgc tgaacctgag tgccgaagac
     5341 atcctcgcca tcggcaggca gaccggcgta atgcacttct tcgcaggttt cgtcatcccc
     5401 gtcatcggct tgggcttcat cgtaccttgg tctgaaatcc gcaaaaactt gggcttcgtc
     5461 gccattgccg tcttctcctg caccattcct tatgtcgcat tggcgatggt caacgaagaa
     5521 ttcccgtcgc tcgtcgccgg cgcgatcggc ctgatggtgt ccgtattcgc cgccaaccaa
     5581 ggctggggct tgagcaagga ccacgccaaa gacccgaatg ccgaaaaagt gccgttcgcc
     5641 caagtcgcca aagcactcgc ccctttgggt atgctgatcg gcatgctggt ggttacgcgc
     5701 atcaaacagc tcggtatcaa aggcattttg accagcaaag aagaatggtt cagcttccaa
     5761 ctgccgtttg atttgtccaa aatcaccgtc agcgactccc tgacgattac cttcggcaat
     5821 attttcggac aagatgtcag cgcgtcttac caaacgctgt acgtcccggc ttggattccg
     5881 tttgtgctga ccgtttggat ttgcatcctg ctgtataaaa ccaaattcaa agatgcctgg
     5941 acgatttatg ccgtaacctt caatcaaacc aaaaaaccgc tgcttgccct gatgggcgcg
     6001 ctgattatgg ttcagctgat gctggtcggc ggcgacaatt cgatggtgaa aatcatcggt
     6061 aaggaatttg ccgcaatggc gggcgaacac tgggtttatt tctcgccgta tctgggcgcg
     6121 atcggtgcgt tcttctccgg ctccaacacc gtgtccaacc tgaccttcgg accgattcag
     6181 cagcaaatcg ccttggatac cggcctgtcc gttaccctga ttctggcgtt gcagtccgtc
     6241 ggcggcgcga tgggcaatat ggtgtgcctc aacaacatca tcgccgtatg taccgtattg
     6301 gatgtgaaaa attccgaagg tgcgattatc aagaaaaccg ttatcccgat ggcgatttac
     6361 ggcgtgattg ccgtcgtcgc ggcaatgatt ttcttcctct aggcgaaacg ccaccatcca
     6421 tagcggcagc acgcggggaa cggaaaccgc cgttccccgt ctgggcggcg cgtcgggcag
     6481 aatcccggtg ccggaacaag cccgccccgc caaacaaatg ccgtctgaaa ccggaaaagg
     6541 cttcagacgg catttttccg ccgcttgcgt tcaggcgtaa tgatgatgcg gcatcttgcc
     6601 gtcaaggcgg tagcgcgtgc cgcaatacgg gcaggcaacg ctgcccgatt ctccttcgca
     6661 caaaggcaaa aacactctcg gatgcccgtt ccactgctcg ttgtcgggtc ccgagcaata
     6721 cagcggcaga ttttccggca acacggaaat ttcctgcaga ttcagattgt ccatttgatt
     6781 ttcctttgcg tggttggcgt gcgcccattt tacgccatcg gcaggctaaa ggatattttc
     6841 ggcgcaaagc cgcaatccgc tataatcccc acttttcaga cggcatacca tgactgcgct
     6901 tacccttccg gaagacatcc gccaacaaga accatccgtt ctgctctata ccctcgtttc
     6961 cgcctacctc gaacacaccg cccaaaccgg cgacgaatcc ctctcctgcc tgtccgacga
     7021 ccagcacacg ctgaccgcat tctgctacct cgacagccaa gtcgaagaag gtggcttcgt
     7081 gcaactcatc gcatccggtt acggcgaata tattttccgc aacccgcttg ccgacagtct
     7141 ccgccgctgg aaaatcaaaa ttacgcccaa aatcatcgac aaagccaaaa gcctctacga
     7201 aaaacacggc gaaaccatcg aaacgctcgc cgacggaggc gcagacatcg aatccctgcg
     7261 caaacagttc cccgactttg aagaatggga cggcgcatac tacgaagccg ccgaacaaga
     7321 cctgcccctg cttgcagaac acatacagtc aaactgggaa accttcgccc atatcgggca
     7381 ggcgtgattg cgtctgtttc cagcccgtgt aaaacagcgt aaaatcggca aacccgaatg
     7441 accttccgtc cccatcaagg agcaagctat gttcttcaag cacatcgaag ccgcccccgc
     7501 cgatccgatt ctcggtttgg gcgaagcgtt caaagccgaa acccgccccg aaaaagtcaa
     7561 cctcggcatc ggcgtttata aagacgcatc cggcgcgaca cccctcgtca aagccgtcaa
     7621 agaagccgaa aaacgcctgt tggaaagcga aaccaccaaa aactacctca ccatcgacgg
     7681 cgttgccgac tacaacgcgc aaacccaaat cctgctgttc ggcaaagacc acgaaatcat
     7741 cgccagccgt cgcgccaaaa cagcgcaaag cctcggcggt acgggcgcat tgcgtattgc
     7801 ggccgagttt gccaaacgcc agttgaacgc gcaaaccatc tggatttcca atccgacttg
     7861 gcccaaccac aacgccatcg ccaaagcggt cggtatccaa gacaaacctt atcgttacta
     7921 tgatgccgcc aaacacggtt tggattggga cggcatgatt gaggacttga gccaagcgca
     7981 aaaaggcgac atcgtcctgc tgcacggctg ctgccacaat cctaccggca tcgaccctac
     8041 gcccgaacaa tgggaaactt tggcaaaact ttctgccgaa aaaggctggt tgccgctgtt
     8101 tgactttgcc taccaaggct tcggcaatgg tttggaagaa gatgcctacg gcttgcgcgt
     8161 gttcttgaaa cacaatacag aattgctgat tgccagctct tattccaaaa acttcggtat
     8221 gtacaacgag cgcgtcggcg cgttcacttt ggtggccgaa gatgaagaaa cagcagcccg
     8281 cgcccacagc caagtcaaaa ccatcatccg taccttgtat tccaacccgg cttcacacgg
     8341 tgcgaacacc attgcgctgg tgttgaaaaa tgatgatttg aaagcacaat ggattgccga
     8401 actcgatgaa atgcgcggcc gcatcaaagc catgcgccaa aaatttgtcg agttgctcaa
     8461 agccaaaggt gcaagccaag actttgattt cattatcgaa caaaacggta tgttctcttt
     8521 cagcggtctg acccccgaac aagttgaccg tttgaaaaac gagtttgcca tttatgccgt
     8581 ccgctccggc cgcatcaacg tcgccggcat taccgacgac aacatcgatt atctgtgcga
     8641 aagcatcgtg aaagtcttgt aaaacaaaag cgcatacgaa atgccgtctg aaccttaacc
     8701 agttcagacg gcatctgttt tttgttaaaa aacaataaac cataacaatt tcaaacaagc
     8761 taaaaaacat ctataaaatc aacccaatat gatgaactca aataaaaaat acgaatatca
     8821 tcattctcaa attcaaaatt tgaacgccac agtattatcc gaagccacaa aaacctttcc
     8881 gccgtcattc ccgcgcaggc gggaatccgg gaatccaatg ctgcaagaat tgattagaaa
     8941 caactgaaac cgagcaaact ggattcccgc ctgcgcggga atgacgaaat aaggcgagtg
     9001 aaactgatga aacaaattga ttaaaaacaa taacttatat tattttattt aaattttcga
     9061 taaaatcaat tttccgtatt caatactgct ccaatcaatt cccgcgcacc gtcgtccgcc
     9121 aatttcttag cgacagcgcg tccgagcgcg tcggcatatt cggcaggggc ttgcgcgtcc
     9181 gcccgcaaca caaccgaacc gtcggggtgt ccgaccaagc cgcgcaaggt cagcaagccg
     9241 ttttcttccg tgcaatatgc ggccaaaggc acttggcagc ttccgcccaa agcgcgtgcg
     9301 agggcgcgtt cggcggtaac gcaggcattg gtaacaccgt ggttcaaggg tttcaaaact
     9361 tcatacaaat cttcgcggtg cgcggcaatt tcgataccca atgcgccttg tccggcggca
     9421 ggcaggctgt cggattccga caaaatcatg cggatgcgtc cgtccaattt cagacgctgc
     9481 aaaccggcgg cagccaagat aattgcgtcg tattcgccgt tatcgagttt ggacaaacgg
     9541 gtttgcacat tgccgcgcaa aggtttgata agcaaatgcg gatagcgcgc acgcaactgg
     9601 gcttcgcggc gcaggctgga tgtgccgaca accgcgcctt cgggcatttc ttccaaacgc
     9661 gtgtattggt tggacacaaa tgcgtcaaac ggattggcgc gttcgccgat ggccgcaagc
     9721 gcgaaacctt caggcaaatc catcggtacg tctttaatcg aatgcaccgc caaatcggcg
     9781 cgcccgtcat ataaagcctg ttccaactct ttgacaaaca agcctttacc gccgactttt
     9841 gacaaagttt tgtccaaaat ctgatcgccg cgcgtggtca tgcccaaaat ctcgacttcg
     9901 caatcgggat acagagcctt cagacggcct tggatatgct ttgcctgcca catagcaagc
     9961 aggctttcgc ggctggcgat gacgagtttt ttcgggttca tgttcaatgc gttcaaagaa
    10021 tggaaacggc gtgaagtcta tcatattttg aacgtttctt cagacggcac cgtcaggtct
    10081 atgttatatt tacacccaca aaacacgaaa cccgccacaa tgaaacgcac caaaaaatac
    10141 cctttcttaa cgttgcagcg gcaacgtttc catttgaact ttgaaaacgc ctcttccgcc
    10201 gccggcatcc cagccgaacg cgatttctac cgctgggcgt ggtctgcctt gaaaaatgaa
    10261 taccgccgcg ccgacatcag cctgattctt ttggacgaag aagaagcccg cgactacaac
    10321 cgcgactacc ggggcaaaga ttacgccacc aatgtcttga gtttcgcgct caacgaaggc
    10381 gaaaccctgc cctgccaagt ttcggaaaaa ctgtacggcg atttgattat ctgcccgcaa
    10441 gtggttttga aagaagccgc cgaacaaggc aaaacacccg agcagcattt tgcccacctg
    10501 accatacacg gtactttaca cctgatgggc tacgaccaca tcgaagacga cgaagccgaa
    10561 ataatggaag ccgaagaaat ccgcctgatg cgggcggcag gcttccccaa cccctaccaa
    10621 gaggacggac attaaaatgg acggcgcaca accgaaaacg aatttttttg aacgcctgat
    10681 tgcccgactc gcccgcgaac ccgattccgc cgaagacgta ttaaacctgc ttcggcaggc
    10741 gcacgagcag gaagtatttg atgcggatac gcttttaagg ttggaaaaag tcctcgattt
    10801 ttccgatttg gaagtgcgcg acgcgatgat tacgcgcagc cgtatgaacg ttttaaaaga
    10861 aaacgacagc atcgagcgca tcaccgccta cgttatcgat accgcccatt cgcgcttccc
    10921 cgtcatcggc gaagacaaag acgaagtttt gggcattttg cacgccaaag acctgctcaa
    10981 atatatgttt aaccccgagc agttccacct caaatccatt ctccgccccg ccgtcttcgt
    11041 ccccgaaggc aaatcgctga ccgccctttt aaaagagttc cgcgaacagc gcaaccatat
    11101 ggcgattgtc atcgacgaat acggcggcac atccggcttg gtcacctttg aagacatcat
    11161 cgagcaaatc gtcggcgaaa tcgaagacga gtttgacgaa gacgatagcg ccgacaatat
    11221 ccatgccgtt tcttccgaac gctggcgcat ccatgcagct accgaaatcg aagacatcaa
    11281 caccttcttc ggcacggaat acagcagcga agaagccgac accattggcg gcctggtcat
    11341 tcaagagttg ggacatctgc ccgtgcgcgg cgaaaaagtc cttatcggcg gtttgcagtt
    11401 caccgtcgca cgcgccgaca accgccgcct gcatacgctg atggcgaccc gcgtgaagta
    11461 agcaccgccg tttctgcaca gtttaggatg acggtacggg cgttttctgt ttcaatccgc
    11521 cccatccgcc aaacataaaa atgccgtctg aaaccgttgc aagtttcaga cggcatttcg
    11581 cttatccgct tattttttgc tcaaagtcag ccccgtccaa ccgaataaca gggtcaaagc
    11641 caaactcaaa tagcagaaaa aggcatacgg cagatattcc caaaccggca cgcccagcgc
    11701 gtggctgatg aacacgccgc atacgctcca cggtacgagc gggttaatca ccgtccccgc
    11761 atcttccagc gtccgcgaca gattgcgcga atgcagaccg agcttatcgt aaacgggttt
    11821 gaacgtttca cccgacaaca aaatactcaa atattgctcg ccgatcagga aattaacccc
    11881 gaccgaagtc atggcaacgc tgaatgtggc acgtccggca ttcgtcaaaa agctgcggac
    11941 ggcatccagc agggaaggaa tcgcgcccag cgcaaacagc agcccgccca aactcatccc
    12001 gagaatcacg atggtttgcg tgaaaaacat actttccaag ccgccgcgcg aaatgagttt
    12061 ggcgatgtct ttaaacgctt cgccttcgag tttgtagccg ccgtaaaacc acgcgccgag
    12121 ctgacgcaga tcgggcgtgc tgtgcagata cgttacggca acggcggcaa tgacggtaaa
    12181 gagcatggcg accacggcat tgacgcgcat caatgccaaa acgaccaaca gtgcaaacgg
    12241 aatcagcgaa tagccgtgca ccaatcccgt ggcttcaagc tggctgcgga aggattcgac
    12301 gctgttcaaa tcctgcgcgg cgacactggg aagaagccac agcatcagtg ccgcactgat
    12361 gagccacgcg ggaatggtgg tgtacatcat atttttgatg tgctcaaaca ggtcgatgcc
    12421 gacgatggac gcggaaatgc ccgtggtgtc ggaaagcggg gacattttgt cgccgaaaaa
    12481 cgcaccggaa acaatcgcgc ccgccgtcat cgccatatcg gcatgaaacg ccgccgccat
    12541 ccccataaag gcaacgccga cggtggcgca ggtggtcagg ctgctgccga tggatacgcc
    12601 gatgacggaa cacagcgcga aagcggaaaa ataaaaataa gtcggggaaa tcagcccgaa
    12661 accgtaatac atcagcgtcg gaatcgcacc gctcatcatc agcgcgctga ccatcagccc
    12721 gatgaagaaa aacaggtaaa tcgcgcccat accctgattc aacgcgccta tcatgccctg
    12781 ctgcatatcg ttgtatttca aaccgcgcgc caagccgtac aaaatcagca cgacgatggc
    12841 ggcaataatg gacatatgcg gcagccactc caatgaaatg atggtatagc ccatcgcggc
    12901 aatcagagcg acgacgacgg caagtgcctc accgcgcggc atatcgagta aggatttgaa
    12961 agcgaacatg attctttgcc cgatttgata taaagttaac ggattctaac acactatacc
    13021 gaatccgccc gacgttttca gacggcattt tagcgtttta caaaagccgg acagaaacaa
    13081 aacggcaatt ttctcaattt aaattaattt ttttgaaata taatttttta tttttttgtt
    13141 tgttgacata taagactttt atggattgaa gtattaaaac ctcggcacac acgccgcaaa
    13201 caccggtacg caaaaatgcc gtctgaaaga tgttcagacg acatttctca agaaaaagat
    13261 tatttgtccg ccctatatcc gtacaccgca aaaaacacga tatacagata acagatgacg
    13321 gaaacgacaa acgaagacat cagggtgtaa gtatctgcca cccagccctg cacgacagga
    13381 acgaccgcac cgccgacaat cgcggtacac agtacaccgg aagccgcgtt ggtaaatttt
    13441 cccaatcctt tggttgccaa agagaaaatc gtcgggaaca taatcgagtt gaaaaaaccg
    13501 atggcaagca gcgaccacat cgccacatcc gcattgccgc tacccgtcgc catcgcgacg
    13561 gcaagcagta cgaccgcagc cgatgcgtta aacgccaaat aacggttggg cgcgaatttc
    13621 gccatcaccg ccgaaccgag gaaacgtccg accatcgcgc cgccccaata gaacgacagg
    13681 taatgcgcgg cagaagcatg atccagccct ttcagataac ccaatacgtt gaccatcaac
    13741 gaaccgatag acacctccgc gccgacatag cagaaaatgc ctgccgtacc gaacacgaga
    13801 tgtttgtatt gccatacgcc ggttttgccg tcgtggttgt gcgcgctttc ctcggcggca
    13861 attttgcgcg cgtcgggcag ccggatcatt ttcacgaaaa cggcaaggat aatcagcagc
    13921 cccgccagtc ccaaataggg aatctgtacg gaagaaatct gttccgcctt gctgacggtt
    13981 tgggttgcgt ccgccaaaat caggaacgcg ccgatttgcg gcgcaatggt cgtacccaaa
    14041 gcgttaaacg cctgaaccag cgtcagtgtt gccgattcct tgccgggttt cgccagcagg
    14101 gtaacataag gattaccggc gacctgaagc agcgttacgc cggaagccaa aataaacaac
    14161 gcgcccaaaa ataccgcgta agaatggctg cccgcagcag gataaaacag caggcatccg
    14221 accgccgtca gcagaaagcc gccgataacg ccgtttttgt agccgacttt gccgacaaaa
    14281 gcccccatcg ggatggacat caccgcatag gcggtaaaga aacagaattg gatcagcatc
    14341 gcctgaacgt aagacaggtc gaaaatttct ttcaaatgag ggataaggat gtcgttcatg
    14401 caggtaataa aacccatcat gaagaacagc gtggtcaaga cgaccaatgg ggacgtatga
    14461 ttgttttgtg attgcgcaga catgatttgc ctttcgtggt gtcggaatat tacccgtttc
    14521 aagaaaatag aaattgacat ttcttaacgg caatgcaaat catactgata tttgaacatt
    14581 ttgcaaactt tatcggatgc aggcaatgcg ccgatctgaa attcaagcag cagaaattaa
    14641 tattttacag ttgcttgcat agattttaat agattttaat agattttaaa aaattccggc
    14701 ggacttgcaa aaaccgttcc caataaaatg aaaatccgcc gccgtatccg gaaaattcgg
    14761 aaatgccgca tcgtggcatc aaaaccggag cggaaagaat gtcgtatgcc cgacaaaaaa
    14821 ggggcatacg gctgtgccgt attttttgca ccgtcctccg cccttcggcg ggg
//
LOCUS       DAWKQB010000039        14866 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.039, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000039 DAWKQB010000000
VERSION     DAWKQB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.039"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(130..825)
                     /locus_tag="WJL14_000885"
     CDS             complement(130..825)
                     /locus_tag="WJL14_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7655979.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(996..1655)
                     /locus_tag="WJL14_000886"
     CDS             complement(996..1655)
                     /locus_tag="WJL14_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ7655980.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(1853..3965)
                     /locus_tag="WJL14_000887"
                     /pseudo
     CDS             complement(1853..3965)
                     /locus_tag="WJL14_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(4151..4756)
                     /locus_tag="WJL14_000888"
     CDS             complement(4151..4756)
                     /locus_tag="WJL14_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7655981.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(4805..5203)
                     /locus_tag="WJL14_000889"
     CDS             complement(4805..5203)
                     /locus_tag="WJL14_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7655982.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            5311..6222
                     /locus_tag="WJL14_000890"
     CDS             5311..6222
                     /locus_tag="WJL14_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ7655983.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTGAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVALELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            6212..7513
                     /locus_tag="WJL14_000891"
     CDS             6212..7513
                     /locus_tag="WJL14_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ7655984.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELNKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            7576..7872
                     /locus_tag="WJL14_000892"
     CDS             7576..7872
                     /locus_tag="WJL14_000892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7655985.1"
                     /translation="MTQYLPFFSLSIIRYAQITATQAATVPIVGKKCCQSVIGGTGQR
                     PHSISAPPNNRYSSISAIQSNNGYFKRCNQKTNHVRTIYLPPFRQRYCTASVQC"
     gene            7906..10056
                     /gene="dinG"
                     /locus_tag="WJL14_000893"
     CDS             7906..10056
                     /gene="dinG"
                     /locus_tag="WJL14_000893"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ7655986.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LTREEGEEAPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEKLNLLRDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKAACPNRA
                     ECPFYLARDMLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVIGLNDALS
                     AARREKEQDSALIDRLTGEFGLFISRIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALQLPDDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            10092..10706
                     /locus_tag="WJL14_000894"
     CDS             10092..10706
                     /locus_tag="WJL14_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ7655987.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALELDDNLVENLERQLGF"
     gene            complement(10801..12669)
                     /locus_tag="WJL14_000895"
     CDS             complement(10801..12669)
                     /locus_tag="WJL14_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7655988.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVMIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRDGALQ"
     gene            complement(12750..14120)
                     /gene="purB"
                     /locus_tag="WJL14_000896"
     CDS             complement(12750..14120)
                     /gene="purB"
                     /locus_tag="WJL14_000896"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ7655989.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIAEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKSLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGLLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            complement(14192..14824)
                     /locus_tag="WJL14_000897"
     CDS             complement(14192..14824)
                     /locus_tag="WJL14_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ7655990.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAASLFAISDDEDEIVAYLSEHGLQTA"
BASE COUNT         3548 a         4110 c         3827 g         3381 t
ORIGIN      
        1 tgttaatccg ctatatattc tgatttaaaa ccataaggct ttaagcaatc atctcttcta
       61 taaagcctaa atacaaaagg ccgtctgaaa tcctattttc agatggcctt tactcattca
      121 atcctcaact tattgcggct ttttctgctc ttcgcgtact ttatccacca actggtcaat
      181 cgtggtcata ccggactgcc agtctttaaa ttcaacttgg tatttgccgc cgacaatcac
      241 agtcggtgtg ccgctgattt ggaatttatt ggtcaactct tccatttgag ccgcacgcgc
      301 ttggctttca ggagcctcaa atgcagccaa tacttttttg ccgtcaaacg ctgtttgctc
      361 ggacagccat tttttcaggg tatcggtatc ggccagattg attttttgat taaccatcgc
      421 atcgaaaata tggctgttgg ctttatctga ttcaccggcc atttccactg cggccgccaa
      481 acgtgccaaa ggtttcattt catcacccca cacgacatgc tcccggcgca tataggtatc
      541 gtctttaaac gttttgatgt gctcgctcaa gaccggctca agatgggcgc aatgcgggca
      601 gaagtagccg aaaaattcca atacttcgat tttaccggcc tgctgttgcg gaataggcgt
      661 agacaataca gtgtagttca caccttcgtt caactcagca ggggctgccg gagcagatga
      721 gctgctttgg gcgctgtctg ccggaacact ggtttcagcc tgtttgctac aagcggccaa
      781 tgccaacagg gtcaatgaag tcaaagctaa ggttttcagt ttcataggta tctcttgtgt
      841 gtcagattaa tgtgcggatt ttatggcatt ttattgaagg cgtgttcgat ttgagtgaaa
      901 aaatgtgttt agttttattt cagcctttat aaaggccgtc tgaaatatgg cttagataat
      961 tagccttatt ttcagacggc ctttgttcta gtaatttacg gtttcggaca ttttgtcttc
     1021 aatccgtcga tgtgttccat taagtcaggt gggacatgat tccacatagg atcaagaata
     1081 aaatcgatac cttcccgtct ggctaatttg gctgctggga caaaatcgga atcaccagaa
     1141 aacaagatga ttttatcggc ttgttttttt aaggtaatag atgaaatatc caagcctata
     1201 cgcatatcta cacctttttg tttaacatcg agcacgacat cgttttccgt aatttgagtt
     1261 gacgggtttt tcagaagatt tttcatgact tggggtttta atatccatgc tgtattgtcc
     1321 gacagcttgc ctaaacgtaa cgctaattta cgcttcttct tcaattcggt atgcagattg
     1381 caacgaaata tggcttccgg agtctttgaa aaatctatgg cacgatgaga tacgggattg
     1441 tgcattttct tttcaaacgg cacgcagtcg taaaagaaaa ttctatataa atcatccatt
     1501 tgatctgtgc cgttgatttt atctttcaga tgcagcatcg aatatcggaa agccaaatca
     1561 gcaattcttt ttgcatcgtg tggattttga gacaggccta atgaccgtac ccgcttaata
     1621 aaaaatgcac cgtcaatcaa aatggcggtt ttcatattgc ttcccctata tttgtcaaag
     1681 atataaaaaa gcccttggga tcggcatacc cgctatgatt taatgggtaa tgcgtactgc
     1741 caagggcact atttcttttt ggattatact gatgtagata aagcctgtca aaaagttcgt
     1801 atgctgaatt tacggattat caggattttc agacggcctt tgcctgattt catcagaaac
     1861 ggtaatttac ccgggcagta tagccgcgcg gattacccgg catagagtcc gaacgccaat
     1921 atttttgatt gaacagattg gctgcggcaa aggtaacgtt aacattttta tggttccagc
     1981 caagcatggc atcaactcgg gcaaagcctg gaagcgtagt cacttcttta tttcttgagt
     2041 cgtaaccgta gcgtttgcct gtaccggtta cgccgatttc gccgtagagg ttttcggtcg
     2101 gggtataacg gaaaaacagg ttgccggtaa cgttgctggt gttattcaaa tggatgccca
     2161 ctcggtcggg attttctttg tcttcaacga ctttcgcctg catcacgccc aacgaaccgc
     2221 gcagatagag tttttttggg gatgatttgc ccgatggcgg acaattccac gccgcgcgaa
     2281 cggtgtttgc cgctaaccgc ataaatataa gggttgtttt ttggatcggg gcggtagcgg
     2341 atattgaagc gttcgatttg gtaggcagac aacgtagtgc tgaggcggtc gtccagccaa
     2401 ctgcttttca cgccggtttc gtattggcgg gtgtactcgg ggtcggcgtt gaacacggcg
     2461 gaagacaacg tatcgatgct caaatagccg ccgcgtccgc cataaggcgc gaagcctttg
     2521 ttatacgagg cgtaaagtgt gtggacggga ttgatgttcc acactgcgcc gatgttgggg
     2581 ctgaacgagt gtccgctgta ttggcggctg ctgccggtga gtttgttttc ggaattaaag
     2641 gtgtatttgt cgtaacggcc gccgaggacg aatttcaaat cgggcgtggc ggagaagatg
     2701 ttttgcacaa agatgccgta ggagtcggct ttgtggcggt tttgggtcag aataggctgc
     2761 aatctgcccg aagccggcca gcttgcgcgg tcgtaggggt tgatggaggc ggaaaaggcg
     2821 ctgctgaaac ccaatgtcgg gttgcggtgt tcgcggctgt aatccatgcc tacggtcagg
     2881 tggttttcaa aacggccgat ggtgtagtcg ccgttgagcg ttaagttgga cgacagggtt
     2941 ttgttgtcgg tctgctgcca ggcgtagtta cgtttgatta agttgccatt ttcgctgcct
     3001 gcatagaaat gatcaaaatc ctgcgccgcc gtgcggtggg cgagctgcca ttgggcacgc
     3061 catttgtcgt tgaaggcgta ttcaaggtcg gaacgccaaa cttgcagctt gtctttgaca
     3121 aaatcgttcc ggtgggcgaa ccccatgcgg taaggcagtc cgaagcggtc gtacacggac
     3181 ttggtcggac tgcggtcggg cgtgcgctcc acattgtcgt aggtgtattg ccccgtccac
     3241 ttcaagccgt tgtcgagttt gacggtaatg ctgggcgaaa ccatgacatt tttgctgtct
     3301 atgccgctgc ggaacgaatt ggcgcgcccg acttcgccgg tgagacggat ggcgacgttt
     3361 ttgttcagca cttcgttgat gtccatattc aggctgcggt ttgcccacga accataaacc
     3421 gtaccgatat tacggctttg tttgaagttg gcgtatttgc tgaccatgtt gatgacaccg
     3481 ccgccgttgg tacgcccata aagcacggag gacggacctt tcaggatttc cacgcgctcg
     3541 atgttggcgg tgctacggcg cacctgcccg ctttcgcgta cgccgtcgcg gtaaatatca
     3601 gatgcgtcgg cttgaaagcc gcgcaggaaa atgctttcgc cgcgcatatc gtaggcggcg
     3661 tcgatgccgg cattgccttc gaggatggaa ctcaaatcgt tcgtgccgta atttttgttt
     3721 ttctggatat tgagcgtatc gatggtttgc ggcgtttctt tgatgagctg tccgttgcgg
     3781 gtaacggcgg cttcgtcgta gttgatgtag cctttgagta cgctggtgtc ggactgtccg
     3841 accacggaaa cggtgggcag agtggcggtg taatgttcac cattgtcctg cgtatcggcg
     3901 gcagcaacag ggaaggaagc aataatcagc gtgggtaata aagctaaatg aaatgatatt
     3961 ttcattttta tactcaattt aacaaaacaa ccgaattata ttgcctcacg gaggaaatga
     4021 gaataatttc ttttaactat attgaacatg atatttgtaa acaaaggtct cagaatgcgg
     4081 aaaactcgcc gcctgatact gaaaaatgcc gtctgaacag ggttcagacg gcattttttt
     4141 gaccgcgaaa ttatgcgccg aacactttca aacgttctgc aactggttca aaggtctttt
     4201 cacctgccgc cccttcaata ccgccgataa ccatttgtgc gcgcaacaac cagttttcgg
     4261 ggatattcca cgctttggca atcgccgcat cgggcaaggg attgtaatgt tgcaggtttg
     4321 cacctacgcc gaccgcggca agtgtcgtcc aaacggcata ctgcaccatc gcgttcgcct
     4381 ggtccgccca aacgggaaag ttggcggcat aagcagggaa ctgctcctgc aaacctttga
     4441 cgacattttg atcttcataa aacaaaatag ttgccgcacc cgccttaaac aggttcaatt
     4501 tttgcgcggt cggttcaaaa ctgtcggcag gcacgacggc acgcagcgcg tcttcgacaa
     4561 attgccacac cttatcatgc tcttcgccaa acagcacgac cacacgggca gattgggaat
     4621 tgaacgaaga aggtgtgtgc aaaacggcgt gttcgacgat ttggacgatt tcatctttgc
     4681 cgacgggcag atttttattt aacgaataaa tggaacggcg gctttcggca gcctgttgca
     4741 gagattgacg ggtcatttct tttcctcaaa aacattcaac aaaatacact ttaggcagtc
     4801 tgattcaggc tttacgcttg aaaaacagcc tgtccagcga ccatgctccg ccgcccgccg
     4861 ccgcgatata gaggaatacg aagcagaaca gcactgcgga ctcgccgccg ttggcaatcg
     4921 ggaacaaagc atttccggaa gcgtgcgcca taaaataggc aaccgccatc tggccggaca
     4981 aaacaaacgc ggcagggcgc gcaaacaggc ccaacaccag caaaatgccg ccgacaattt
     5041 ctaaaatacc ggcaagcagc aacagcccgc cgggcgaacc gctgcccatt tcaatgggga
     5101 aggcgaagat tttcgacgta ccgtgcaaca aaaacaggta ggcggttacg atacgcaaaa
     5161 cagaaagcaa aaccggttgg atacggttgc agcaatcgga cataaaagct cctttgatat
     5221 ttgtcttcat ttcaggcggt agagtataga tgtttcccct tttcaaatat atactgcttt
     5281 tattgataca acttttccac acaagaaaat atggacaccc tgttcagcct caaggttttc
     5341 cgccaagtcg tccaaagcgg cggcttcacc cgcgccgccg acgcgctcgg catctccacc
     5401 gcaatggcaa gcaaacacgt cagccacttg gaaaacaccg tccaagccaa actcctgcac
     5461 cgcaacagcc gcaacctcag cctgaccgga gccggggaag aatactaccg gcaatgcagt
     5521 tacgcgctcg acacgctcga cgatgccgcg caaaaagccg ccggagggac ggaaaaaccg
     5581 caggggctgc tgcgcgtaac gatgccgctg tggtttgccg gcagccagat atgcaactgg
     5641 ctggcggaat accgcgaacg ttatcccgaa gtggcattag aactgatttt ggacaaccgc
     5701 cacgtcgatt tgattgccga aggcgtggat ttggcgttgc gcgtttccca aaccctgtcc
     5761 ccgtcgctga ttgcgcgccc actggcggaa atcgaatttg ccctgctcgc ctcgcccgat
     5821 ttcctgaggc gcaacggcgt gccggaaacg ccggaagaag tggcggggct gcccgccgtc
     5881 ctgccgacct acaccaacca gcagaaactc gacctcaccc gcaaatcgga cggcaaaaaa
     5941 taccggcttg aactgacccc cgtcatccgt accgacaaca cgctgatgat gcgcgaaatg
     6001 attaaggcgg gcgcgtgcat cggttatcag ccgctttggg cggcggaaca cgatttgcgc
     6061 tgcggctcgc tggtgaggct gctgcccgaa tataccgtcc cgaccgaccg cctgaatgcc
     6121 gtttatgcgg acagggcgtt cttaagcgcg aaagtccgca gcttcatcga ttttctgaac
     6181 gaaaaaatcg ccagcaggaa aggctgccga aatgccgtct gaaaccgccc gcccccttta
     6241 tgcggacacg tgcgccgcac acacgctcgt ctggttccgc caaaacctcc gcatccgcga
     6301 caacgccgcc ttatgcgccg ccgttgccga aggtttgccc gttatcggta tttggattga
     6361 cgatgccgaa acagacaacc ctcgccgcgc cgcgttctac cgccaatccg ccgccgaact
     6421 cgcccaaggg cttgcagggc gcggcatccc gctctacacg gcggcatcgc ctgccgagct
     6481 cgtccggctc gccgtccgcc tcaatatccg cgccgtcatc gccgacgaat cccatacttt
     6541 tgccgacaaa ctcgccgaca acgccctttg gcatgaattg aacaaacacg gcatcgcgtt
     6601 aaccttcgtc aatgaccgcg ccgttttcgg caaaaccgac ctgatacccg acggcggcac
     6661 ggcacacacc gatttcgacc gctaccgcga agtatggctc gaccgctttt ccaagcagcc
     6721 ccccgccggt ccggacctat tcgcggcata ccgccaaccc ttccccgaaa acctttccgc
     6781 cccgccgcct gccgcccttt cagacggcat cttcctgccg caaaacagcg gcgaaacggc
     6841 ggcttggcgg cagtggcggc ggtttctcga acaggcggat tcctactccg ttttaaagga
     6901 tttcccctcg cgcaaacaca cttcgctgat gggcgcgtat ttgagtgccg gctgcatctc
     6961 gccgcgcctg ctcgcgcggg aaagcctcga acgccgtctg aacgcgtggg cggacaacat
     7021 catccgccgc gattttttcc ttcaacttgc cttgcagcac acggatgacg acccttcaga
     7081 cggcaatcct gaacacaccc tgcgcctgac gctttggcag cagggccgga ccggcattcc
     7141 gattatcgat gccgcgatgc gctgtttgca caaaaccggc agcctccacc ccgccctgag
     7201 acgcttgagc gcggattttt tctgccacgt tttaaacctc ccccgccgcg aaggcgagat
     7261 atggtttgcc cgacagctga ccgatttcga tgcagcaatc aaccaaggca actggcggct
     7321 tgccgcctca cggcacacct gccccgacat tgccgccgcc gcacacaaga ccgaccccga
     7381 cggcaccttt gtcagacggc acattcccga gcttgcccac ctgtccgccg acaccgtcca
     7441 cacgccttgg cggtttgcct gttcggtcga tacccacggc tatcccgccc atcctgtcgc
     7501 cggtattccc taagcggtac ggcaacctaa aacaaatgcc gtctgaactt ccgtttcaga
     7561 cggcattgcg tccgaatgac tcaatatctt ccgttttttt cactttcaat catcaggtat
     7621 gcccaaatca ccgccaccca agccgccacc gttcccatcg tcggcaaaaa atgctgccaa
     7681 tccgtcatcg gcggtacagg gcaacggccg cattcgattt ccgcgccgcc caacaaccgg
     7741 tacagcagca tatccgcaat acaaagcaac aacgggtatt tcaaacgctg caaccaaaaa
     7801 accaaccacg tccgcacgat ttacctcccc ccattccgac aacgatattg taccgcttcc
     7861 gtgcaatgtt aaaataaccc cacttctacc gccgtacaaa acgccatgct caccgattta
     7921 gaaaaaaacg ccatccgcga ccattaccaa aacatcggca aaaacctgcc cggtttccgt
     7981 ccgcgtgctt cgcagcggga aatgattgcg gcggttgcca acgctttttc gcggacgttg
     8041 acgcgtgaag aaggcgaaga agcacccaaa cgtgagggcg agagcattgc cgtgatcgaa
     8101 ggcccgaccg gcgtgggcaa atcgttggcc taccttttgg ccggcggcat catggcgcaa
     8161 acgcgcggca aacggctgat tgtcagcagc gcgacggtgg ctttgcagga gcagttggtt
     8221 gaccgagatt tgccgtttct ggtcgaaaaa agcggtttgg agctgacctt tgcgcttgcc
     8281 aaagggcgcg gccgttatct ttgtccgtac aaactctatc aactgacgca aaacaacgcc
     8341 cagcaaaacc tgcttggttt tgaagcgccg gaagtgttgt gggacagcaa acccaagccc
     8401 gaaaaattga acctgctgcg cgacatcgcc gacgaatttt ccgcccgacg gttcaacggc
     8461 gaccgcgaca cctggccgga aaaaatcgat gacgcgattt ggctcaaagt taccaacgac
     8521 cgccacggct gcctgaaagc cgcctgtccc aaccgcgccg aatgcccgtt ttacctggca
     8581 cgcgatatgt tggaaaccgt cgatgtcgtc gttgccaacc acgatcttct gcttgccgac
     8641 atcagtatgg gcggcggcgt gattctgcct gcgcccgaaa acagtttcta ttgcatagac
     8701 gaagcgcacc acctgcccaa aaaagccctc agccgttttg ccgccgaaca ttcatggaat
     8761 attgccgttt ggacgctgga aaaactgccg caactgaccg gcaagattgc cgcgctgacc
     8821 gataaagccg aacttgccaa ccttgccgac gaagccgcct catccttact cgacagcctg
     8881 cacgaatggc agttccattt ggcggaagag ccgtctttga gcttggggcc gtctgaaaac
     8941 gacaggcgta ccaacagcga accgacttgg ctgtgggaag acggcaaaat ccccgaaggc
     9001 ctcgaaacca ccgtttccaa caccgccatt gccgcacgca gcctgctcaa acacgttatc
     9061 gggctgaacg atgcgctttc cgccgcgcgc cgcgaaaaag aacaagacag cgcgctcatc
     9121 gaccgcctga ccggcgagtt cggccttttt atctcccgta tcgaacaaat tagtgcggtt
     9181 tgggatttgc tctccaccgt tcccattgaa ggcgaagagc cgttggcgaa atggataacc
     9241 cgccgtgccg acgataaaaa cgactatatt ttcaacgcca gccccatcag cagcgcgtcc
     9301 caccttgcca acagcctgtg gcggcgggcg gcaggcgcag tgttgacctc cgccaccctg
     9361 caatccttgg gcaactttaa cctgatgctg cgccaaaccg ggctgcaatg gctgcctgaa
     9421 accaccaccc tcgccctcaa aagccccttt gactttgaaa aacagggtga actctacatc
     9481 cctcccatat acgccagccc caaagacccc gaagcccaca ccgccgccat catcgagtgg
     9541 ctgcccaagc tcatttcgcc cggcgaagcc atcggcacgc tcgtcctgtt ttcctcgcgc
     9601 aaacaaatgc aggatgtcgc cttacaactg cctgacgact atctgccgct cttgctcgta
     9661 caaggcgaat tgcccaaagc cgttctcctg caaaaacacc accgggccat agaagaaggc
     9721 aaagccagca tcatcttcgg actcgacagc tttgccgaag gactcgacct gcccggcacc
     9781 gcctgcgtgc aagtcatcat cgccaaactt cccttcgcca tgcccgacaa ccccatcgaa
     9841 aaaacccaaa accgctggat agaacagcgc ggcggcaacc ctttcatcga aatcaccgtc
     9901 cccgaagccg gcatcaaact catccaggcc gtcggccgcc tcatccgcac cgaacaagac
     9961 tacggccgcg taaccatcct cgacaaccgc gtcaaaacac agcggtacgg ccaacaatta
    10021 ttagccggcc tgccaccgtt taaaaggata gggtaaacct accgccctgc ccgcaacaga
    10081 aaggaaaaat catgaacttc acccgactgc tcaaccaagt cttaagcacg gttcaaaaaa
    10141 aaggcaacac cttctccgac agcccgctca attcattcgg cggaggcgcg ctggttgccg
    10201 gtgtcgcctc catgctgctg aacggtaaaa accgcaaaac catcaccaaa atcggttcga
    10261 ccgccgcttt gggctacctc gcctaccggg gctatcagat gtggcagcaa aacaaagggc
    10321 gggcaaccgt aacacaaagc gatttccaac ctgccggaga aaccgaagaa acatacagcc
    10381 gcaccgtatt gcgcaccatg atagccgccg ccgcttcaga cggcatgata gacgaagccg
    10441 aacgccggac tatcgaacag gaaagcggca cagaccccga aactgctgca tggctcgccg
    10501 ccgaataccg cctgcccgca agcatcgaag acatcgccgc cgccgtcggc aacgatgagg
    10561 cgttggcggc ggaggcctat ctggcggcaa gactggtatg tgccgatttg tcgcggaaag
    10621 aaaccgtctt cctcgcccgc ctgtcgcagg ctttggaact ggatgacaat ctggtggaaa
    10681 acttggaaag gcaattgggg ttttgattca aaattccctc ccgcagggga aggaaaataa
    10741 aaaccgccgc cgccccgaaa ggcaaacggc ggcacaaacg atatcggacg gcaaggcagg
    10801 ctactgcaat gcgccgtcgc gagaagattt aagcaggcgt tcgtgtttgt gaaattcccg
    10861 ccccatataa taagaaccga aataggtcag gtggtcttgg tcgccgtaaa gatagcggcc
    10921 gtatatttcg accgtgtttt tgggcaggta tttttgtgcg tccacccaat gcacattggg
    10981 aatatcttta atcaaatcaa agaccgcctg attgctcttg ccgatgtcgc ccatagcctg
    11041 aatggggcgg agatattggt ttgcggcaaa tcttttcaat ttttcctccc tcaggggcga
    11101 acggctgatt gatgtgttgt ttgcaaaaac atagacgggt ttgacggcgg ctatcctttt
    11161 gacggtttcc ctgaatcggg ctgggaaccc gggtattagg aaggattgcg cttcaaatct
    11221 cggcacgggc tggccgccca tcctcaaatc atagaattgg gcaatgaaaa cggcttcggc
    11281 tttttcaact tcatcccggt attttcgaca taacgggttg tctgccagct tctcatctac
    11341 ccaaaccaaa cactccgaat cgagggacag gattttggct ttccaccctt cccggctgcc
    11401 gacataatcc agaaaccccc gcaggtgtcc ggcgtgcgag tcgccgaggg tcaggacggt
    11461 ttccggaaaa tgattttccg cagcaagggg cgcgccgggc aacgggcgga ggtgttcctg
    11521 tttcaatatc ccccttgcgt acaggttgta accgacaagt atcagggacg gggcgagata
    11581 gaggcagaaa aatgcctttt tgaaggtcat cttccgtttt ctaagcggct gttcaatcaa
    11641 ataataactc aacagggaaa atccggccgt caacgcggca accgccgata cggcaggcag
    11701 tccgagctgt ttgtcgcctg taatgtaatg ggcgaaagca ataaaaatcc aatggtacag
    11761 gtatagggaa taagagattt tgccgacaaa tacgatgggg cttgccgaca ggatgcgggt
    11821 cggaagtgtc ccgtattgca tactccggat aagcagtgcc gtcagcaggc agggaaggag
    11881 cagggtcatt cccgggataa acggattgtg tttgtcaatc acgaacaggc aggcaagcaa
    11941 tgcgccgaag cagagtgatg aaagcaactg ccgttttcca tttgctgttt gccgtctgcc
    12001 gttttgcgtt tgcccgtaaa ccgccagcag cgaacctgcc aacagctcgg gaaacctcag
    12061 tgtcgaaagg taataagtat tgggttggtt gagaatatcg gtataaaacc cgcttggcaa
    12121 aaacgatgtg gcagtcaaaa tcagaaatag gatgatgctg atgttacgca gcacccgtag
    12181 cgattttgtt tttttgcagc aaaatatcag caaaagagga tacaggaggt aatactgttc
    12241 ctctactgcc aaagaccaga tatgcagtac ggggttctcg tcggcactca aatcgaaata
    12301 cccctgctga aaccccagat aaatattgga caagaaaacc gcagaaagct ccacggtttt
    12361 ccgcatttgg ttgaaatctt cgtaaaggaa gatttgagag gcaatcaccg aagccagcga
    12421 cacggccgca ataaaagcag gataaatccg cttaatcctg cgggtataaa aatcccggaa
    12481 agaaaaagaa ccgttctgta tttcagaaag aatgatgccg gtaatgagga atcctgagat
    12541 gacaaagaaa atgtccaccc ccaggaatcc tccgggcagc cagcggttat tcaggtggaa
    12601 aatcatgacg gatagcacgg cgacggcccg caatccgtca atttccggtc tgtatcggac
    12661 agcttgcata aatatcgccc ctgtatgtcc cgttatctta aaaatgccgt ctgaacgcgc
    12721 gttcagacgg catcggtttc agtaaacgct caaatccgtt tcgccaacgc ttcagccttg
    12781 cccacataca gcgcgggggt cagctcaagc aatttggctt tggcttcggc ggggatttcc
    12841 agcaatccga taaagccttt cagcacttcg ggcgtgatgc cgcctttgcc gcgcgtcagg
    12901 tctttcagct tctcgtaagg attggccacg ccgtaacggc gcattacggt ttgaatcggc
    12961 tcggcgagca gctcccaagt ggcatccaaa tcggcggcaa gcgcggcggg gttaggttcg
    13021 agcttgttca gaccgcgcag gtgggcggcg aaacccaaca cggcatagcc cacgcccacg
    13081 cccatattgc gtaagacggt gctgtcggtc aggtcgcgct gccaacggga aatcggcagt
    13141 ttttccgcca aaaaacccaa tacggcgttt gccataccaa ggttgccctc ggagttttca
    13201 aagtcgatgg ggttgacttt gtgcggcatg gtggaagaac caacttcgcc cgctttgact
    13261 ttttgtttga agtaccccaa agaaatgtag ccccaaacgt cgcggttaaa gtcgatcagg
    13321 atggtgttga tgcggctgag ggtttggaag aattcagcca tgtagtcgtg cggctcgatt
    13381 tggatggtgt aggggttgaa ggtcaggcca aggctgattt cgacgaagtt gcggcagtgg
    13441 gtttcccaat cgacgtcggg ataggcgacc atgtgggcgt tgtagttgcc gaccgcgccg
    13501 ttgattttgc cgaggaactc ttgcgcctga aggcttttaa actggcgttg caggcggtac
    13561 acgacattgg cggtttcttt gcccaaagtg gtcggcgtgg cgggctggcc gtgggtgcgg
    13621 ctcatcatcg ggacggcggc aaggtcgtgc gccatagcgg tcagtttttc gatgatttct
    13681 gccagtttgg gcagcaaaac agcctcacgc gcttcttgca gcattaaagc gtgggacagg
    13741 ttgttgatgt cttcgctggt gcaggcgaag tggatgaact cactcacggc ggcgacttcc
    13801 ggcacttcgg caaaacgttt tttcagccaa tattcaatgg ctttgacatc atgattggtc
    13861 gtggcttcga tggctttgac ggcggccgcg tcttccaatg aaaagttttc aatcaccgtg
    13921 tcgatttcgg caagcgtttc ggcactgaag ggcggcactt cggcaatctt cggctcggcg
    13981 gcgagggctt tgagccagtt taattcgact ttgacgcgcg ctttcatcag gccgtactct
    14041 gagaaaatcg ggcgcaatgc ttcaacggat tgggcataac ggccgtctaa aggggaaagc
    14101 gaggcgatgg ggttgatcat ttcggcatcc tgttcgggaa agacatcaaa aattgttaaa
    14161 ttgtttgcaa ttatacatca ctctcaggac gctatgccgt ctgaagcccg tgttccgaca
    14221 gatacgcaac gatttcgtct tcatcgtccg atatggcgaa caaagaggct gccccttcgg
    14281 aaatcagacc gcgcgccaaa agctgcgcgt ttatccactc cgccaagccc gaccaaaacg
    14341 cctttccgac caaaacaatc ggacgcggcg gcactttgcc cgtctgcacc aaggtcagga
    14401 tttcaaacaa ttcgtccagc gtcccgaagc cgcccggcat cacgacatat gcttgggaat
    14461 agcggaaaaa caccgccttg cgttcggcaa aacgggaaaa ccgcaaggcg atgtcctgat
    14521 acggattcgg tttctgctcg tgcggcaaaa cgatgttcag ccccaccgaa accgacttcc
    14581 cggcaaacgc gcccttgttt gccgcctcca taatccccgg cccgccgccc gaaatgacgg
    14641 caatgcccga atccgacagc cgccgcgcca gacggcagga gaacgcataa tccgcatgat
    14701 tctgcggcgt gcgcgcgctg ccgaagatac tgactgccgg aaacacgccc gccaatgctt
    14761 cgtctgcctg cctgcgttcg gcatcataac gtgcctgctc cggcacacgg tttgtattct
    14821 ccattccatc ctccgttcaa aaacagcgat tgtacaccgt caaaaa
//
LOCUS       DAWKQB010000040        14671 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.040, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000040 DAWKQB010000000
VERSION     DAWKQB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.040"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..961)
                     /locus_tag="WJL14_000898"
     CDS             complement(110..961)
                     /locus_tag="WJL14_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7655991.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVEDWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEEDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAVGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            complement(1058..2263)
                     /gene="tet(B)"
                     /locus_tag="WJL14_000899"
     CDS             complement(1058..2263)
                     /gene="tet(B)"
                     /locus_tag="WJL14_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HEZ7655992.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIVGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2345..2962
                     /gene="tetR(B)"
                     /locus_tag="WJL14_000900"
     CDS             2345..2962
                     /gene="tetR(B)"
                     /locus_tag="WJL14_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HEZ7655993.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLNGFM"
     gene            3225..4739
                     /gene="katA"
                     /locus_tag="WJL14_000901"
     CDS             3225..4739
                     /gene="katA"
                     /locus_tag="WJL14_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7655994.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            4908..5088
                     /locus_tag="WJL14_000902"
                     /pseudo
     CDS             4908..5088
                     /locus_tag="WJL14_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220242.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6095..6538
                     /locus_tag="WJL14_000903"
     CDS             6095..6538
                     /locus_tag="WJL14_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ7655995.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKTAVVEYYSNNGVFPKNNASAGVASASEIVGKYVAKVDVSGNTITSTMKTSG
                     VNKDISGATLVLEGAQKSGSFTWTCKAGSSNGVKDKFLPSSCRAK"
     gene            6952..7452
                     /locus_tag="WJL14_000904"
     CDS             6952..7452
                     /locus_tag="WJL14_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7655996.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            7904..8470
                     /locus_tag="WJL14_000905"
     CDS             7904..8470
                     /locus_tag="WJL14_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ7655997.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLAAGGALGGLF
                     GKRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(8904..10940)
                     /locus_tag="WJL14_000906"
     CDS             complement(8904..10940)
                     /locus_tag="WJL14_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ7655998.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAAYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLENALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YAGEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVVRPPEYNRFANSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(11028..11900)
                     /locus_tag="WJL14_000907"
     CDS             complement(11028..11900)
                     /locus_tag="WJL14_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ7655999.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            12170..14560
                     /gene="gyrB"
                     /locus_tag="WJL14_000908"
     CDS             12170..14560
                     /gene="gyrB"
                     /locus_tag="WJL14_000908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ7656000.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         3645 a         3852 c         3874 g         3300 t
ORIGIN      
        1 tcaaaaacag aaatcccaaa ccctcggcat tcccgccaaa cccccgtcat tcccgctttc
       61 gcagaaatgc cggcttttgg tttgttgtct ttataagatt attcggcaat tattctgcgg
      121 ttttgcgttt gtgtgctgcc ggaatctcaa aggattctct gtatttggca atggtgcggc
      181 gagaaacctc catgccgcgg aaagccagca ggttggcgag cgcctcgtca gaatacggcg
      241 tgggtgtgga cgaggcaaaa ccgacggcgg gaaagggatt gagcgaagca atgaggtcga
      301 gtgcggcttc gagtgtgccg ctgtcggttt ggggcaggcg tttttttatt cgcgcgaggg
      361 tttggctgcg gttgccgtca atgctgtcga gggcgtttcg gacgatatgc agggcggagg
      421 gtttggcagc acattcgccc gatctttcta tctgcagtat cagcgattcg ttcaaatcgg
      481 cggcggccac gcctgccggg tcgaattttt tcaatgcggt cagggcttgt ttcagcattg
      541 cttcatccaa catccactcc aagggcgtat ggtcgaggat gtcttcgatg ctgtcggtca
      601 gataaccctg ctcgtcaagg aaatcgataa ggatgtggac acaggcggct tcttggtcgg
      661 aaagcgggtg ttcgcacact tgcgcgtgca ggtattgctt gaaatcttct tcgccggcga
      721 tgttggacag catatcttcg ccttcgtctc cgccgatttg gcgcgcgggc gcagtgtaat
      781 ggctgaattc ggcatcggaa aattcatccg tgtctttgcg ttcgagcagg gggttgtccg
      841 acagccagtc ttcgacctcg cgttcaagtt cgatacccga catctgcaat acgcgcaaag
      901 attgttgcag ccgctggttg agctgctggg tctgtttgag ctttattccg agtaacgtca
      961 tgataatgcg agagaattat tcttttggcc tgtcggcggc agatgatgcc gggaaacatc
     1021 attataagcg gaataacatc atttggtgac gaaataacta agcacttgtc tcctgtttac
     1081 tcccctgagc ttgaggggtt aacatgaagg tcatcgatag caggataata atacagtaaa
     1141 acgctaaacc aataatccaa atccagccat cccaaattgg tagtgaatga ttataaataa
     1201 cagcaaacag taatgggcca ataacaccgg ttgcattggt aaggctcacc aataatccct
     1261 gtaaagcacc ttgctgatga ctctttgttt ggatagacat cactccctgt aatgcaggta
     1321 aagcgatccc accaccagcc aataaaatta aaacagggaa aactaaccaa ccttcagata
     1381 taaacgctaa aaaggcaaat gcactactat ctgcaataaa tccgagcagt actgccgttt
     1441 tttcgcccca tttagtggct attcttcctg ccacaaaggc ttggaatact gagtgtaaaa
     1501 gaccaagacc cgctaatgaa aagccaacca tcatgctatt ccatccaaaa cgattttcgg
     1561 taaatagcac ccacaccgtt gcgggaattt ggcctatcaa ttgcgctgaa aaataaataa
     1621 tcaacaaaat gggcatcgtt ttaaataaag tgatgtatac cgaattcgat tgcgtctcaa
     1681 cccctacttc ggtatctgta ttatcacgtg tatttttggt ttcacggaac caaaacataa
     1741 ccacaaggaa agtgacaata tttagcaacg cagcgataaa aaagggacta tgcggtgaaa
     1801 tctctcctgc aaaaccacca ataataggcc ccactattaa accaagccca aaacttgccc
     1861 ctaaccaacc gaaccacttc acgcgttgag aagctgaggt ggtatcggca atgaccgatg
     1921 ccgcgacagc cccagtagct cctgtgatcc ctgaaagcaa acggcctaaa tacagcatcc
     1981 aaagcgcact tgaaaaagcc agcaataagt aatccagcga tgcgcctatt aatgacaaca
     2041 acagcactgg gcgccgacca aatcggtcag acatttttcc aagccaagga gcaaagataa
     2101 cctgcattaa cgcataaagt gcaagcaata cgccaaagtg gttagcgata tcttccgaag
     2161 caataaattc acgtaataac gttggcaaga ctggcatgat aaggccaatc cccatggcat
     2221 cgagtaacgt aattaccaat gcgatctttg tcgaactatt catttcactt ttctccatca
     2281 ctgataggga gtggtaaaat aactctatca atgatagagt gtcaacaaaa attaggaatt
     2341 aatgatgtct agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
     2401 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
     2461 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
     2521 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
     2581 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
     2641 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
     2701 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
     2761 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
     2821 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
     2881 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
     2941 acaacttaat ggttttatgt agggattttg attcgtttcg ataacaaaaa acaaacgaaa
     3001 atcaaaaacc gattgcttat aacaatatta aatcgatttc atagttttaa tagcaaaaat
     3061 cttagcgtat agtctcatcc atagttttta caaaaggaaa acaaaatgtc gattcaagaa
     3121 atttattgca atcaagaaac cggttacgaa tacgctttcc gccaaatcgt actgtaagcc
     3181 gtttgtcgta tcttttgatt aaccaaggaa aaggaacacg agctatgact acctccaaat
     3241 gccccgtaac ccatctgacc atgaacaacg gcgcgcctgt tgccgacaat caaaacagcc
     3301 tgaccgccgg tcctcgcggc cctctgctgg cgcaggattt gtggctgaat gaaaaactcg
     3361 ccgacttcgt gcgcgaagtc atccccgaac gccgtatgca cgccaaaggt tcgggcgcgt
     3421 tcggtacgtt taccgtaacg cacgacatca ccaaatacac ccgcgccaaa atcttcagcg
     3481 aagtcggcaa aaaaaccgag atgttcgccc gtttcaccac cgtggcaggc gaacgcggcg
     3541 cagccgatgc agaacgcgac atccgcggtt ttgcgttgaa gttctatacc gaagaaggca
     3601 actgggatgt ggtcggcaac aacacgcccg tgttcttcct gcgcgacccg cgtaagttcc
     3661 ccgacctgaa caaagccgtc aaacgcgacc cgcgcaccaa tatgcgctct gccacaaaca
     3721 actgggactt ctggacgctg ctgcccgaag cactgcacca agttaccatc gtgatgagcg
     3781 accgcggcat ccccgccggc taccgccata tgcacggctt cggttcgcat acctacagct
     3841 tctggaacga agcaggcgag cgtttttggg tgaaattcca tttccgcacc caacaaggca
     3901 ttaaaaacct gaccaacgaa gaagccgcca aaatcatcgc cgacgaccgt gaaagccatc
     3961 agcgcgacct gtacgaagcc atcgagcgcg gcgagtttcc gaaatggacg atgtacatcc
     4021 aagtcatgcc tgaagccgat gcggaaaaag tgccgtatca tccgtttgac ttgaccaaag
     4081 tttggccgaa aaaagactat ccgctgattg aagtgggcga attcgagttg aaccgcaatc
     4141 ccgaaaactt cttcgccgat gtggaacaat ccgccttcgc accgagcaac ctcgttcccg
     4201 gtgtcggcgc cagcccagat aaaatgttgc aggcgcgttt gttcaattac gccgacgcgc
     4261 aacgctaccg cttgggcgtg aacttccgcc aaatccctgt caaccgtccg cgttgccctg
     4321 ttcacagcaa ccagcgcgac ggacaaggac gcgccgacgg caactacggc agcctgccgc
     4381 actacgaacc caacagcttc ggccaatggc agcagcagcc cgacttcgcc gaaccgcctt
     4441 tgaaaatcaa cggcgacgcg gcgcactggg actaccgcca agacgatgac gactatttca
     4501 gccaaccgcg cgccctgttc aacctgatga acgacgcgca gaaacaggca ttgttcggca
     4561 acaccgccgc cgcaatgggc gacgcgcccg acttcatcaa ataccgccat atccgcaact
     4621 gctaccgttg cgatccggca tacggcgaag gcgtggcaaa agccctcgga ctgactgtcg
     4681 aagacgcaca agccgcccgc gcgaccgacc ccgcactggg tcaggccggt ttgctgtaag
     4741 ggggcattat gtggatggaa attgaagaaa tcctgtcccg cgccgtccgc taccggaaat
     4801 aaccgggcat aaaaatgccg tctgaaacgt catttgaccg cttcagacgg catttccaca
     4861 ccccaccccg catttttata tggtcgcgca tcgcagtggg acgtagggtg ttcgtagggt
     4921 gggcttcagc ccaccaatca caccaatcac accaatcaca ccaatccgcc acctcgacaa
     4981 atccgccacc tcgacaaatc cgacaaatcc gacaatctta ccggtgccgg caaacctatg
     5041 ccgtctgaag ttggcggagg cggtgggctg aagcccaccc tacagcccgc cattcccgcc
     5101 ccacagccaa cttttccgcg tcattcccgc gaaagcggga atctagaacg taaaatctaa
     5161 agaaaccgtt ttttccgata agtttccgta ccgacaactc tagattcccg cctgcgcggg
     5221 aatgacgaaa tttttgtttt tgattttttg tttttgggga atgacgggat ttgagattgc
     5281 gggcatttat cgggtaaaac ggaaattaag cgttgcgaaa atttatccga aatcacagta
     5341 acttttccgc gtcattcccg cgaaagcggg aatccaggtt cgttgagttt cagccatttc
     5401 taataaattc ttgcaacttt gagtttctgg attcccgcct gcgcgggaat gacgaaattt
     5461 cagtttttga ttttttgttt ttgcgggaat gacgggattt gagattgcgg gcatttatcg
     5521 ggaaaacagc aacctctcct ccgtcattcc cacgaaagtg ggaatccagg ttcgttgagt
     5581 ttcagtcatt tctgataaat tcttgcagct ttgagtttct agattcccac tttcgtggga
     5641 atgacgggat acgggttttc gtgcggacag atccgtcatt cccgcgcagg cgggaatccg
     5701 gacccgtccg cacggaaact tatatttcgt cattccgcca atcccacccc acccccaaaa
     5761 aaaccaaaaa aaacccaatc tgacaaaatc tgacaaaaaa cgtcagcccg aaactgacaa
     5821 aaattgtcac aattgacaaa aaaacgtcag cccgccgcat tattccgccg caggccgcgc
     5881 cgcgttgata taaaaaaata ccaataccaa acagacaaat aaaaaaatat tctgtaaaat
     5941 gccgagctga accccgtttt ggcacgccgc ttgctggaaa gaaggcgaac cgaaacaaaa
     6001 aagaccacgg tcaaatacat tacgggttta cgtttgcggc ggatgccgca aatttccaat
     6061 caaacttaaa cattttcaaa caggagtcat ccaaatgaaa gcaatccaaa aaggtttcac
     6121 tctgatcgag ctgatgatcg tcatcgccat cgtcggtatc ttggcagccg tcgccctgcc
     6181 cgcataccaa gactacaccg cgcgtgccca aatgtccgaa gccctgactt tggcggaagg
     6241 tcaaaaaacc gcagtggtcg agtattattc caacaacggc gtgttcccga aaaacaatgc
     6301 ttctgcgggt gttgccagtg cttctgagat tgtaggtaag tatgtggcta aggtagacgt
     6361 ttcgggtaat accattacgt ctacaatgaa aacttccggt gtaaacaaag atatcagcgg
     6421 agcaaccctt gtactggaag gcgcgcaaaa atctggctcg ttcacgtgga cttgtaaagc
     6481 gggttcttca aatggtgtga aagacaaatt cctgccgtct tcctgccgcg ccaaataagg
     6541 acaatgaccg ggtctgaccc ggccgtgaaa acaaatgccg cccgaaccgc tcgggcggca
     6601 ttttctattc ccctttgccg ctgtagggtg ggcttcagcc caccattcca ccaatcccac
     6661 caactccacc acatcaagga tttttggtga accgttggga ttcggcgggt ttcgggtttc
     6721 ggcggggatt ggcggaatgg ttggtttggg ttgggtggga atggttggat tggcggaatg
     6781 gtgggctgaa gcccacccta cgaaacaccc tacgaaacac cctacagcct acacatcagc
     6841 cctacgccct ccctacccat ttccctgcaa atcccctaaa aatcctcccg cacgccctga
     6901 aaacaaggaa taatacaggt ttccgcccat cgggctaaaa aggggataaa tatgtcaaat
     6961 taccggaggg acttcacgcg cggggcgcac tattttttca cattggcttt gcaagacagg
     7021 acgaaggatt acctgatacg gcatatcgac aggctgcgcc tcgcctatcg tgaaacgaaa
     7081 acacgttatc cgtttgaaac ggcggcaatt tgtattttgc cggatcacct gcatttcatt
     7141 atgcggctgc cggaaggcga ccacgattac ccgatacgga ttgcatattt gaaaacccgg
     7201 ttttcccaac tcatccccaa agagtgccgc gcccccaacg ggagtaaaac aaaaaaacgc
     7261 gaagccggca tttggcagcg caggttttgg gaacacctga tccgggacga acaagattta
     7321 aacaaccacc gggattatat ttatttcaac cccgtgaaac acgggtatgc ggcgagtgtg
     7381 aaggaatggc gttattcttc cttcttcaaa gacgttgaaa aaggcattta ccctttggat
     7441 tggggagggt aaagctcccg ccgccgccaa aatccgcagg acggacgcgg ggcagatgag
     7501 tcgggcggga cgtagggtga ggcgtaggtg cgtagggtgg atgtgtaggg tgggcttcag
     7561 cccaccaatc caaccacctc cgccaatcca accattccca ccaacccaaa ccaaccattc
     7621 cgccaatccc cgccaaaaac cgaaaccgcc gaatcccgac ggtttgccaa aaacccttga
     7681 tgcggtggaa ttggcgggat tggatgagtt ggtttggcag aattggtggg ctgaagccca
     7741 ccctacaacc cgccctaagt cccacctacg ttccatccga cgcatccgcc ctacggttcc
     7801 ggattcccct gcgaaccgcc ccgcgcacga aaactgcttt acgcggcgtt tttcgcgttt
     7861 tgctgttttg ctataatccg ccctttttaa ggacgggtgc ggtatgggtt tttatgcttt
     7921 gctcttgatt gcgctgggga tgtcgatgga tgcgtttgcc gtcgcgctgg caaagggtgc
     7981 ggcggtcagg atgcccccgc gcaaaattgc agcaacggct ttggtgttcg gctcggttga
     8041 ggcactcacg ccgctggcag gctgggtagg cggtttttat gccaaaccgt ttatcagcga
     8101 atgggaccat tgggcggctt tcgtcctgct gggcgggctg ggtctgaaaa tgatgcgcga
     8161 agggctgtcc ggcaaggcgg aagatgtgcg cgaaagcaaa cgggaaagcc tatggatgac
     8221 ggttttgact gcttttggaa ccagtattga ttccatgata gtcggggtgg gcttggcgtt
     8281 tatggaggta aacatcgcct ttgccgccgc aatcatcggt atggcgacga cggtgatggt
     8341 ggcggtcggg ctggcggcgg gcggggcgtt gggcgggctg tttgggaaac gtgcggagtt
     8401 tgccggcggg ctggtgctga tcgccatcgg cacttggaca ctcttgtcgc acttggggct
     8461 gatcgggtaa ggactgccaa aaaatgctgt ctgaagcgtt gtttgaccgt ttcagacggc
     8521 attttttatt ttttgggcgt agggcgaatc cgtaaggtgg gatgtaaggt gggatgtagg
     8581 gtgggcttca gcccaccaat cccaccacct ccgccgctcc cgccaaatcc gccattcctc
     8641 gccgaaaacc gaaaccgccg aatcccgacg gtttgccaaa aacccttgat gcggtgggaa
     8701 tggtgggaac ggtgggattg gcggaggaga tgggattggt gggctgaagc ccaccctacg
     8761 cccactgttt ttgatttttt gtttttgcga gaatggcggg attttggttt gctgtttttg
     8821 tgaaaatgac gaaaccgcgc aaatgctgat gccgtctgaa acactgtttg attgtttcag
     8881 acggcatttt ttgttcacat cgatcaagcc gcgttgtcga agccgctgtg gcgcaagagt
     8941 gcgtctatgc tcggttcgcg tccgcggaag gctttgaagg attctgccgc gctgcgcgat
     9001 ccgccgacgg cgaggatttc ctgccaaaag cgtttgcctg tggcggcgac atcgtcgctt
     9061 tcttcaaagg cggcgtatgc gtccgcgctc aatacttccg cccacgcgta gctgtaatag
     9121 cctgcggaat agccgcctgc gaagatgtgg ccgaagctgt tggcgaagcg gttgtattcg
     9181 ggcggtcgga cgacggcgac ttctttgcgc acgctgtcta aaacctgttg ccagtttttc
     9241 agacggcctt cgtcgtcttc gctgtaaatc atcatatcaa agagggcgaa ctccatttgg
     9301 cggacgagga acattccgcg ttggaagttt ttggcggcga gcattttgtc gaagagttct
     9361 ttcggcaggg gaacgccggt ttcttcgtgg gcggacattt gtgccaagac gtcgtattcc
     9421 cagacgaaat tttccataaa ctggctgggc agttcgaccg cgtcccattc tacgccgttg
     9481 atgccggata cgcccagttc gtccacttgg gtaagcaggt ggtgcagccc gtgtccggtt
     9541 tcgtggaaga ggatgaggat ttcgtcgtgg ctcaaacgcg cttctctgcc gccgacgggc
     9601 ggggcgaagt tgcagacgag gtaggcggtg ggcagttgca gcgtgccgtc tgaaaaacgg
     9661 cggcgacctt tgtagtcgtt catccacgcg ccgccgcgtt tgccttcgcg tgcgtacaaa
     9721 tccatataaa cgccgcctat ggtttcgccg ttttgttgca attcaaaata gcgcacgtct
     9781 ttgtgccaga cggggacggt tttttcggta aatccgatgc cgtagagttt tttgatttgg
     9841 gcgaacagtc cgtttaatac tttgccgacg gggaagtatt ttttgacttc ggtttcgctg
     9901 aatgcgtatt tggcttcgcg cagtttttcg ccggcgtagc ccaagtccca cggttgcaaa
     9961 tcggcgaggc cgaggctttc gcgggcgaag gctttgactt cggcgaggtc tttttcggcg
    10021 tagggtttgg cgcggcgggc gaggtcgtgc aggaagttta aaacttgttc gggggtgtcc
    10081 gccattttgg ttgccagcga caattcggcg tagtttttga agccgagcag tttggcggtt
    10141 tgcagggcgt tttcgagcgt gcggtcgatg ttggcggtgt tgtcgaattt gccgtcgtct
    10201 gaaagctcgc tggcgcgggt aacgtaggcg cggtagattt gttcgcgcag ttcgcggttg
    10261 tcggcgtatt ggatgacggc gaggtagtgc ggaatctgca aaccgatttt gtagcctgtt
    10321 ttgccttcgc tttgcgcggc agcggcaaac atggcgagcg cgtcttcggg aatgccggca
    10381 agcggtgcgg catcgtcaaa gtaaatgccg aacgcgtcgg tcgcgtctag gacgttttgg
    10441 gagaatttgg cggaaagttg cgcgccttcg gtttgcagtt ttgccagttc tgcctgctgt
    10501 tcgggcggca gttccgcgcc gctgaggacg aaatcgcgca gatcgtggtt gagtttggtt
    10561 ttttgcgcgt gggagagggt gtcgaactcg ggggagtttt tgatggtttt gaagcggttg
    10621 tacagctcga tgtcttgtcc gatttcggtg aagaagacgg taatttcggg cattaattca
    10681 ttgtaggcgg cgcgcagttc gggcgtgtcg gcgacggagt tgaggtgcga caccacgccc
    10741 caaatcctgc cgacgcgttc ggtgatgccg gtcaggggtt cgacagtgtt tgcccagccg
    10801 gtgtgcgttt gggctttgat ggcggcgatt tgttcgcgcg cttcggcaat ggcggtttgc
    10861 agggcgggtt tgatgtcttc ggttttgatt tgatcaaaac ggggttcttc gcccaaatgg
    10921 agcagtgcgt tgtcagtcat aagatgggtt tcctttgcgt gttgtgcttc agacagcata
    10981 tcggcaatat gccgtctgaa gccggaaatg gggtcggtca gggaaagtta aagtgtttcg
    11041 gagccggcgg ggtaatttaa caggcggtat tgtacgcgca aaaattcgtc cataaccgcc
    11101 tgttggattt ccagccgctt tgaaacgggg gaggcgaagc ggacgatgat gcggtatgcc
    11161 ttgtcatcgt acggcacgcg ggtaacgcgc ggtctggcgg cgggcgtgat aaacagtttt
    11221 tccgcctgca cgttttccaa atgccgttgg atggcgggga tgtagggcgc gcacaagggc
    11281 tcgagtacgg ctttcagacg gcatacggct tcatccgaat ccaaatggat gggaacgggg
    11341 atttcgaccg tatggatgac atagtcgccc aaaatattgt cgcggcgcac ggggtggctc
    11401 aacaacaggc tgttggggaa agaaacggtg gttcccgcaa gctgtccgac caaggggttc
    11461 ggaccgacct gcatcatcag cgtgttcaac aggttgatgt cgaccacgcg cccgcgcagg
    11521 ccgttgattt cgatatagtc gccgaccgag tattgctggg tggcagacct taaaatactg
    11581 cccgacagac acataatcag ttccttcgtc gccacgacga ccgccgccgc caccgcaaac
    11641 atcgacaaag ccagcgtttg gatttgcgcc gaccagataa atgccagcga aaacagcacc
    11701 aaaagcagcg ttatattgcg gctggcaacc aaaaaccgcc gcttgctttc gatgccgaaa
    11761 tccggatgcc gtttgaagtg gatattcaac agaagggcgc gcgccagcag caaagccgca
    11821 accgccgcca cggattcgac cgcctccgca cgtatcggga cggcaccgag ccaagtgtcc
    11881 aacatattcc atatttccat ttccgcgccc ctgtccgaaa agtatagggt gtagatttta
    11941 gtggcaaaaa aacgcttttg ccactttacg ggaaaatccc cgacctgatg ccggaggcaa
    12001 acgtaccgtt tcatcttttc ccggcggcac actgatgcgg ataacgctga attaggcggc
    12061 aaaatcggct ataatacaca attatatatg ccgtcccgcc cgctttttct acaaggtgcg
    12121 gacttgtttt ggcggcatcg caaaatcttt tcaaaatccg gcaaaaaata tgactgaaca
    12181 aaaacacgaa gaatacggcg ccgacagcat tcaggtgctc gaaggcttgg aagcggtacg
    12241 caaacgcccc ggtatgtaca tcggcgacac gcaggacggc agcggcctgc accacatggt
    12301 gttcgaagta ttggacaacg ccattgacga agcactcgcc ggacattgcg acaaaatcac
    12361 ggtaacgata cacgccgacc attccgtcag cgtcgccgac aacgggcgcg gtatgcccac
    12421 cggcatccac ccgaaagaag gacgctccgc cgccgaagtc atcatgaccg tattgcacgc
    12481 gggcggtaaa ttcgacaaca acagctacaa aatctccggc ggcctgcacg gcgtgggcgt
    12541 gtccgtcgtc aacgcgctgt ccgactgggt tacgctgacc atctaccgcg acggcaaaga
    12601 acacttcgtc cgcttcgtgc gcggcgaaac cgaagagccg ctgaacgttg tcggcgattc
    12661 cgataaaaaa ggcacgaccg tgcgcttcct cgccagtacg gaaaccttcg gcaacgtcga
    12721 atacagcttc gacatccttg ccaaacgcat ccgcgaactt tcgttcctga acaacggcgt
    12781 ggacatcgaa ttgaccgacg agcgcgacgg caaacacgaa agcttcgccc tttccggcgg
    12841 cgtggctgga ttcgtgcaat acatgaaccg caaaaaaacg cccttgcacg aaaaaatctt
    12901 ctacgcgttc ggcgagaaag acggcatgag cgtcgaatgc gcaatgcaat ggaacgacag
    12961 ctatcaggaa agcgtgcagt gcttcaccaa caacatccct cagcgcgacg gcggtacgca
    13021 cctgaccgcg ttgcgccaag tgatgacgcg caccatcaac agctacatcg aagccaacga
    13081 agtcgccaaa aaagccaaag tggaaaccgc cggcgacgat atgcgcgaag gtttgacctg
    13141 cgtgttgtcc gtcaaactgc ccgaccccaa attttcgtcc caaaccaaag acaaactggt
    13201 ttccggcgaa atcggtcccg tcgtcaacga agtcatcagc caagccctga ccgacttcct
    13261 cgaagaaaac cctgccgaag ccaaaatcat caccggcaaa atcgtcgatg ccgcccgcgc
    13321 gcgcgaagcc gcccgcaaag cccgcgaaat cacccgccgc aaaggcgtga tggacggctt
    13381 ggggctaccc ggtaaactcg ccgactgcca agaaaaagac cccgcattgt cagaacttta
    13441 tctggtcgag ggcgattctg caggcggttc cgccatgcag gggcgcgacc gcaaattcca
    13501 agcgattttg ccgcttaagg gcaagatttt gaacgtcgaa aaagcgcgct ttgaaaaaat
    13561 gctcgccagt caggaagtag ccgctttgat taccgcgttg ggcgcgggca tcggcaaaga
    13621 agaattcaat gccgagaagc tgcgttacca ccgcatcatt atcatgaccg atgccgatgt
    13681 ggacggcgcg catatccgca ccttgctttt gaccttcttc taccgccaaa tgcccgaact
    13741 ggtcgagcgc ggctacatct atatcgccca gccgcctttg tataaagcga aatacggcaa
    13801 acaggaacgt tacctcaagg acgagttgga aaaagaccaa tggctgctcg gtcttgcctt
    13861 ggaaaaagcc aaaatcattt cagacggccg caccatcgaa ggcgcagaac ttgccgacac
    13921 cgccaaacaa ttcctgttgg caaaaaccgt catcgaacag gaaagccgct tcgtagacga
    13981 actcgtcctg cgcgccatgc tacacgcgtc gcccattgat ttgacgtcgt ctgaaaacgc
    14041 cgataaagcc gttgccgaac tttccggtct gcttgacgaa aaagaagtcg ccctcgaacg
    14101 catcgaaggt catgaaggac accggttcat caaaatcacg cgcaagctgc acggcaacgt
    14161 catggtcagc tacatcgaac ccaagttcct caacagcaaa gcctaccaaa ccctcaccca
    14221 aaccgccgcc gcgctcaaag gcatggtcgg cgagggtgcc aagctttaca aaggcgaaaa
    14281 cgggtacgac gcggacagct ttgaaaccgc tttggacatc ttgatgagcg ttgcccaaaa
    14341 aggtatgtcc atccaacgat acaaaggctt gggcgagatg aaccccgagc agctgtggga
    14401 aaccacgatg gatcccgccg tgcgccgcct gttgaaagtg cgcatcgaag atgccattgc
    14461 cgccgacgaa gtgttcgtta cgctgatggg cgacgaggtc gagccgcgcc gtgcctttat
    14521 cgaaaacaac gcgctgattg cccaaaatat cgacgcataa gtgccgtttt aaaaaaggag
    14581 acgggcatcg tgccgcgtct ccttttggtt tgtcaaacgg aagccatgcc gtctgaaacc
    14641 gcctttcgga gcaaaaccat gatcagcatt t
//
LOCUS       DAWKQB010000041        14560 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.041, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000041 DAWKQB010000000
VERSION     DAWKQB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.041"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(338..4507)
                     /locus_tag="WJL14_000909"
     CDS             complement(338..4507)
                     /locus_tag="WJL14_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ7656001.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4562..6409)
                     /locus_tag="WJL14_000910"
     CDS             complement(4562..6409)
                     /locus_tag="WJL14_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ7656002.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTLARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6974..8203
                     /gene="sstT"
                     /locus_tag="WJL14_000911"
     CDS             6974..8203
                     /gene="sstT"
                     /locus_tag="WJL14_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ7656003.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMVFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8315..9808
                     /locus_tag="WJL14_000912"
     CDS             8315..9808
                     /locus_tag="WJL14_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7656004.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGINKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHMENGRPSLSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9883..10098)
                     /locus_tag="WJL14_000913"
     CDS             complement(9883..10098)
                     /locus_tag="WJL14_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656005.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLKAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(10091..10306)
                     /locus_tag="WJL14_000914"
     CDS             complement(10091..10306)
                     /locus_tag="WJL14_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ7656006.1"
                     /translation="MKEYKVIIYQESLLSSLFFGAAKVNPIKFSEFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10513..11856)
                     /gene="argG"
                     /locus_tag="WJL14_000915"
     CDS             complement(10513..11856)
                     /gene="argG"
                     /locus_tag="WJL14_000915"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ7656007.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGIAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAITGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEDAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKQ"
     gene            11981..12781
                     /locus_tag="WJL14_000916"
     CDS             11981..12781
                     /locus_tag="WJL14_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ7656008.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFVFFKSLLESKGLKLNQVQYLP
                     DEPDLLVKQLRRSFSDGMPTFVTGGIGATPDDHTRQAAAAALDLPVVRHAQAAGFIEG
                     VTLKRGEPLDSPEHAQRLKMADFPQGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYLGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLEGLGAELKNRVN"
     gene            12821..13807
                     /locus_tag="WJL14_000917"
     CDS             12821..13807
                     /locus_tag="WJL14_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ7656009.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYRDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14464..14560
                     /locus_tag="WJL14_000918"
                     /pseudo
     CDS             14464..14560
                     /locus_tag="WJL14_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3113 a         3677 c         4355 g         3415 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacctgtgta
       61 tcgttgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacggcgttg
      121 cctcgcctta gctgaaagag aacgattctc taaggtgctc aagcaccaag tgaatcggtt
      181 ccgtactatt tgtgctgtct gcggcttcgt cgccttatcc tgatttttgt taattcacta
      241 tatcgattct gttcccttgc aggcggacgg cggcggcgga cggtggaaaa gcaggttttc
      301 aaatgtccgg tttggcggcg cgccgtctga aaaccgctta ttttcctttg ctgtttccgg
      361 cggagtcttt tttgtccgaa ccggagaagc ggtcgaaacg tatggtgtat gtcagctcgc
      421 cgcccgacga acggctgccg atacgggcaa ccgcctgtat ggcgcgggtc agccggtaaa
      481 tcagtttgac ggactgttcc gcgctggaga tgctgtattc gtagccgatg tagagtttgc
      541 cggtcagttg tttgccgacg gtcagcacct gttcggcggg gttgagttcg ccggtttgcg
      601 cgttgcggct gcgcttgctg gtaaagccca aatcatccac cagcccgatg cggtcgttga
      661 tttgcccggc aagcagcgcg ccggcggctg cggacagggc ggcattgtcg ccgctgctgc
      721 cactgccggc gcggttgagg atgagccagg agagcttgtc tttttcactc atcggttcgt
      781 ttgccgtcag cgtaatgcgc ggactgttga ggctgcccaa tatttccacg cccgcaccga
      841 cgggggaaag gcggcgttcg gcgcggatgt tgaggttggg gtcgttgagc gggccgacaa
      901 aggagaccgt gcctttggta atgtccaaat cctgcccgta tgccttataa cgccctttga
      961 tgacgcggac cgtgcccacg ccccgcacgc ttccgcccga ttgggcggtc agggtcagtt
     1021 tgccgcctat ggtaacgtcc gcgccgtagc cggcgaagcg gatgccgtca ttgaggtcta
     1081 aagtcaggtt catattgacg gggagcggtg ccgccgcctc ttttttgact tcgcctaata
     1141 cgacgacatc gtcgccgacg gacggcatcg aggatttttg cgaaccgaac agcccctgat
     1201 ccgttttaat catcccggta acggatatgc ctttttgcgg cgaatagcgc aggcgggtgt
     1261 tgccggaaac cgtcaggcgg cggttggggc gggacaggat gcggtatttg tcgaacaccg
     1321 cgccgatatc gacatcgggt ccgctgtttt ccataccgac cgtaccggag agttccgccg
     1381 tcccttcgtg ccggaatttc aggctgtcga ttacccattt cctgcccgcg atatgcgaac
     1441 gcagcgagcc gttgtccaag atgatgcctt gggtttggtt gcggtaatag agtttgtcgc
     1501 cgttgatgct gccgcccaag tgcggatcgg cgatgctgcc gccgagggtt acggcggcat
     1561 tcaggctgcc tttgacggtt tgtccgaccg gtaggaagtt gcggaatact tcggcatcgg
     1621 caacggtcag gttgagcctg ccgcccaaag gcgcggtatc gaaagagcgg ctttgcccga
     1681 cggtgatgtt gccgttgatt ttcccgtagt tgctgctgcc gttgacggcg gcattgacgg
     1741 acggagagcc gacccgtccg ccgatttgcg cggcggcatt caggctgccg gtaatgtttt
     1801 gcgcggcggc gggcagaaag ggcttcaatg tgcccaagtc gggaagggag gcggtaatcc
     1861 tgccgccgag cggtgcattt gccatattgc cgccgaaggc gttgccgatg tccaaatcgg
     1921 cgttaatccg cccgaaacgc gcgccgccgt caagcaggat tccgatacgg tcgttttgaa
     1981 agcgcgtttt cagggaaaat gcgttcaaac ccaaagcctg cccgccgggc aatacggcat
     2041 cgccgctttg ccggctgata ttgaggtagc cgcgcgcgtt tcgcccgtag gcgacatccc
     2101 agtcgccgtt taaaaccaga ttgtgttcga agggcggttt gaagaaattg tgcaactcgg
     2161 cgatatgcag accgtgtgcg ccgccttttg ccgatatgcc ggttttttta tcccaagaaa
     2221 agtgttgcag gttgaggctg ccgcccattg cctgccaatt tgccgcactt gccgccacgc
     2281 gttccgcacc ggcttcgagc gtcatacggt tttgcagctt gaggttgaat gcgccgccga
     2341 tgtcgaggat gccgatgctg cctttccatc gggtaagttc cctgttgatg ccgcctgaag
     2401 cgtccaaatc gaatttgaac ggtttgccat ccagcgtcat ggcggcgtgt gtgcggatgc
     2461 ggtgctgcac gcccgtgccg tccagcatca ggtcggcggt atcgacaacc gccgctccgc
     2521 ccgacagcga aaggcggctg cctttgatgt cggcgcgtat cgggcggctt gtgtcgggcg
     2581 aacctttgag cgtgaaatcg agcgaacgga tgtctgccgc cttgccgatg tgcaggttgc
     2641 gcgccgcgcc ggaaaggtcg gtttcaaagg ttcggatgcc gccgtccaaa tcgccggaaa
     2701 ggtgtccgcg tacatttaaa gaccccgcga gtccgaaacc gaaacgggat aaatcgggtg
     2761 cggtgatgtt gaggttgagc cggtcgcctt ttttgccgaa gccgccgtct gttttaataa
     2821 tgttccgccc cagccgcaaa tcgacggcgg cacgcggaag gtggcgggac tcgtaaacaa
     2881 tgtcggcact gccggcaatc ggtacgccgt tgaacgtgcc gggtaaaaac cgcattttgc
     2941 ctgtgaattt ctcttttgcc agttcgccgg caaggtttat tgagccgttg atattgcctg
     3001 ccggaagttg cggattgatg cgcgaagggt cgaatgcgcg ggaacggatg tccagcttga
     3061 gcaggcggtc tttaaacagc tcgagatagc cttgcgcggt caggctgcct tgcccggcgg
     3121 cgatgttgac ggtgtcgagc accagtttcc gctgtccgtt tgcggggtcg cttgcaatgg
     3181 cgaggctgcc gtccgtgcgc gccgtgccga tgccgagttg ccaagagatt ttgggcgagg
     3241 cggtcgtgcc accgatgccg atgctgccgt ccaacctgcc tttgaacgcg gtttgcagta
     3301 cgtcttccgc gccgacggag ttgatgccta tatttaaatc gaggatgtct ttttcggtgt
     3361 cgattttgcc cgacagcctg atgccgcccc gtccgagcag ggcggcggac gtattgccga
     3421 tatgcaccgt gccgtcctgc cggatgacaa agccgcctaa aacctgacgg acggggatgc
     3481 cgttgcggtc ggcaaagccg gctttggtgt tttccaaatc gagcgaacct tccagcgcga
     3541 tgccgtctga aaacgacggg atggcggtca ggtcgaaatt cagcccggca tcgggcaggg
     3601 agggcacgaa ggcggccggg ttgatgttga accctttgac cagcacttct tccaatgttt
     3661 tatccaatga ttcggcaaac gggtggatga cggattttcc cgagaggcgg atattgccgc
     3721 cgtcgatcgc cagttcggcg cgcacatcct tcaggctgcc gctcagccgc gccgtactgt
     3781 gtatggtttt gccttcgagt ccgcctttgg tgtaaatggc ggtatcgagg gcaaacggtt
     3841 ttttcaagcc gaccgaggct gaccccgacg aactgctcca cggcgtgtcg gcggccttca
     3901 ggtcaaggcg gtgtcctttg cggtcgtaac ggtatgaagc atccagccgt tcgagataga
     3961 cggtttgttt gtcaaaggct ttgcccatgc tgattttgcc cgtctcgaag cggtcgagat
     4021 agacggcggc aggcaggtct atgctgtcgg gaaggctgag cggcgggcgt tcttctttag
     4081 gcggagtcgg tttggtaacg atggcgatgt cgccggcgga aatttcggta atgtgcaggc
     4141 tgcggcgcat cagttcggac ggtttccacg cgaagcggaa gcggctgatt ttaaggtctg
     4201 ccccctcggt ttctatcgac cagttgtcgc cgtcgaagcc gtcgagcagc gtgcctttga
     4261 ggttttggga ggaaatgttt acgccgaacc aagacgggat ttggtacagc ccgaagcgca
     4321 aacctgcttc cgtaccggcg agccagccga ggaaacatac tgccaaaatc aggacagaca
     4381 gcagtgccgc cgacagcttc agcagcgggc ggcgtttttt tgccggcggg gtggggcggt
     4441 gttcagacgg cattttgcgc gtgccgtttt cggtcggatc ggtatctgtc ggtgcggtat
     4501 cggtcataat gtctgtttca aaatagtttg ctgcaatgcc gtctgaagcg gccgtatcgg
     4561 tttagaagcg cgttcccaag ctgatgtgcc agcggatttt cttatcgctg tgcccgtagg
     4621 cgatgtcgaa ggaaaacggc gcgagcgggc tgaaccagcg cacgcccagt cccgaaccgt
     4681 gtttcagctt catacgtttg aaattggcgg cggcgtcgcc catatcgtgg aacaccgcgc
     4741 cggaaagggt gcgcgtaaac ggcagttggt attccaggct gcccaccaag agggcgcgtt
     4801 cgggcaggac cgatccgttc gggccggcaa gtccgatgct gtcgagttcg taaccgcgca
     4861 cggaagacgc gccaccgctg cggaacatca gccccgaggg gacatcggca ttgtcgcggg
     4921 caagggtgta acccgcttgt ccgcgtatga tgaacgtgcc gagtttcttg ttttcgggcg
     4981 tgaagaaata acctgcgcgg gcagaggtgc ggatcagcgc ggtggaggac aggaatgtgc
     5041 ccaaagtcgt accgattttg ccgtcgaggt aatggccgtt ttcgggatgc agcacgttgt
     5101 tgagcagctg gcgtttccaa gaggcggtca gcatcgtggc gtggctgttg cccaaatcga
     5161 tatccgagcc ggggattttc cggccttctg cgagaaactc tgcccccagc ctggcatcga
     5221 tgcccgcgcg gtcgcgcaca taccagatgc cgccggagaa ggcgcgtttt tcgaggtttt
     5281 gggtggtcga acggttgtag gaaacgttgc ttgtccagta gttgccccga tagttgcgcg
     5341 gctggctgat gccggcggca agcgtggttt cgtatttgtc catatcccag acgaccgagc
     5401 cgatatagcc tttgttgaag aggttgtaat agtcgtaggc gattttgccg cccaaaccgt
     5461 attccgaatc gaggcggatg ccggtttcga gcttgtggcg tttgacctcg gttacgctga
     5521 ctttgacggg gacgcggtcg ccttggagac ggtcgaaatc ggcttgtacg gacgcgccgg
     5581 aataatgccc gttttgttcg agtgcctgtt ggaagtcgag cagcaggtcg aggtcgtagg
     5641 gcgtgcccgg ttggaagcgc gccaagccgg agacgatttg ttcggggtaa cgctgcgtgc
     5701 cggtaatttc aaagtcgccg aaggcgatgg ggcggccgct gtccacgacg acgttcaaat
     5761 cggcggtggc ggtatcgggg ttgacggccg cccgggtgtt gccgagcttg gcaagcgggt
     5821 aggctttgcg cgttaccgcg ccgaggacgg aagttttgct gttttcccaa ctgtcctgat
     5881 cgaaatcact gcctaccggc tgctgccagt tttccagcgc gttgcggtag tattcggcga
     5941 ggttgccgtc tgaaaggatg tcgccgagga tggcgacgcc gacgttggcg attttggtgc
     6001 gcgggcccgg tgtgatgtgt accgtataag ctccgtcttt ttccgtcagg ctgactttgc
     6061 tgctgaaata gcctttgctg cggagcattg ttttaacgtt gtccggtgct tcttcggcga
     6121 ggaagcccgt ctgttccttg tccaatactt cttcctgctg ctgcgtgatg agcggcaggt
     6181 gttcttcgac catatctttg atttcactat cctgcgtgtc gatgcggacg gggaatttgg
     6241 gttttaattt aactgattcg gtgtcggggc ttttgttttt gaacaatgcg aaacccgccg
     6301 ccttgttttc ggaaaggtcg gcggcaggcg cgtatgcgtg cggaaagaaa aataaagccg
     6361 gcaggagcag ggcggtcggt ttaatcatca tggtacgggt gtcgtgcatc ggtgtggcgg
     6421 ttttcgattc taacactgtt cggacagggt ttcgaacggc cgtttttgtg ttgttcgtac
     6481 ggggcgggac gcttcagacg gcatactgcc gccggtattt cggaggttgc ggcccgccgg
     6541 aaatgtatct gcttgtttta aggtattttg tttctaatcc gcatatggaa aacaggctgc
     6601 gtttcggaat agtgtcggaa aataaacggg attaaataaa tttattttcg ttcaataaat
     6661 taaaccgtcg cgccgaatga taaagtaaag cctgtccggc actgcatagg gcaaatcaac
     6721 gcaaccttca tagtggatta acaaaaacca gtacggcgtt ggctcgcctt agctcaaaga
     6781 gaacgattct ttaaggtgct gaagcaccaa gtgaatcggt tctgtactat ttgtactgtc
     6841 tgcggcttcg tcgccttgtc atgatttttg ttaatccact ataaaacaca gccgaaaaat
     6901 aaaaaaaaca cagagaatga gttttctttt aaaatacaac attttttaac atatcacaac
     6961 ataaggaaga attatggcgt tcggcaaatc cttatttcat gcgatcggcc gcgtcagcct
     7021 ggtcaggcag attgccgccg gtttggcgtt gggcatcgta atcggttcgg tttccccgca
     7081 actgggcttg gcggcaggct tgttcggcag cctgtttgtc ggcgcgctca aagcggtcgc
     7141 gccggttttg gtatttattt tggtggcggc cacaatcgcg cagcaccaaa aaggcaacaa
     7201 ggcgcatatc aggccgatta tcgtccttta cctcatcggt acgttttccg cagccctgac
     7261 cgccgtcatc gccggtatgg ttttcccgac gcacattgtt ttggcgggcg cgggcgatgt
     7321 gtccgccgcg ccgccttccg gcattgtgga agtattgaaa tcgctgctga tgaacctggt
     7381 cgccaacccg attaacgcga ttgccaatgc caactatatc ggcattttgg cttgggcttt
     7441 ggttttgggc gcggcgttgc ggaatcacgg ttcggacgtt acgcggcagg tcgttgccga
     7501 tttggcggaa gcggtttcca ccgtcgtgaa atggattatc cgttttgccc ctttgggcat
     7561 tttcgggctg gtgtcgtcca caatcgcgga gacgggtttc ggcgcgctgg cgggttatgc
     7621 gaagctgctt gcggtactgt tgggctgtat ggtgtttatc gcgctggtgg tcaatcccgc
     7681 catcgtgtgg tggaaaatcc gccgcaaccc ttatccgctg gtgtttacct gcctgcgcga
     7741 aagcggcgtg tatgccttct ttacccgttc ttccgccgcc aatatccccg tgaatatggc
     7801 tttggcgaaa aaactggggc tgcacgaaga cacttattcg atttccatcc cgttgggggc
     7861 gaccgtcaat atgggcggcg cggcgattac gattaccgtt ttggctatgg cggcggcgta
     7921 cacccaaggc atacaggttg attttgccac cgcgctgctg ttgagcctgg tggcgacggt
     7981 cagcgcgtgc ggcgcgtccg gtgtggcggg cggttcgctg ctgctgattc cgctggcgtg
     8041 cagcctgttc ggcatcagca acgatgtcgc tatgcaggtg gtcgccgtcg gctttatcat
     8101 cggcgtgatt caggattcgg cggaaacggc gttgaattct tcaaccgatg ttttgtttac
     8161 cgccgccgcc gatttgggca gacagagaaa ccgggcggaa taaatcaagc tatgccgtct
     8221 gaagcccttt tggcgttcag acggcatatt tttacaattt tatgggcata tggcggaaac
     8281 aataccggct gccttgaata tgaaggagat gatgatgttt aaacgcagtg tgattgcaat
     8341 ggcttgtatt gttgcccttt cagcctgtgg gggcggcggt ggcggatcgc ccgatgttaa
     8401 gtcggcggac acgctgtcaa aacctgccgc ccctgttgtt actgaagatg tcggggaaga
     8461 ggtgctgccg aaagaaaaga aagatgagga ggcggtgagt ggtgcgccgc aagccgatac
     8521 gcaggacgca accgccggaa aaggcggtca agatatggcg gcagtttcgg cagaaaatac
     8581 aggcaatggc ggtgcggcaa caacggataa tcccgaaaat aaagacgagg gaccgcaaaa
     8641 tgatatgccg caaaatgccg ccgatacaga tagttcgaca ccgaatcaca cccctgcacc
     8701 gaatatgcca accagagata tgggaaacca agcaccggat gccggggaat cggcacaacc
     8761 ggcaaaccaa ccggatatgg caaatgcggc ggacggaatg cagggggacg atccgtcggc
     8821 aggggaaaat gccggcaata cggcagatca agctgcaaat caagctgaaa acaatcaagt
     8881 cggcggctct caaaatcctg cctcttcaac caatcctaac gccacgaatg gcggcagcga
     8941 ttttggaagg ataaatgtag ctaatggcat caagcttgac agcggttcgg aaaatgtaac
     9001 gttgacacat tgtaaagaca aagtatgcga tagagatttc ttagatgaag aagcaccacc
     9061 aaaatcagaa tttgaaaaat taagtgatga agaaaaaatt aataaatata aaaaagacga
     9121 gcaacgagag aattttgtcg gtttggttgc tgacagggta gaaaagaatg gaattaacaa
     9181 atatgtcatc atttataaag acaagtccgc ttcatcttca tctgcgcgat tcaggcgttc
     9241 tgcacggtcg aggcggtcgc ttccggccga gatgccgctg attcccgtca atcaggcgga
     9301 tacgctgatt gtcgatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc
     9361 gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgtccg gcggatcgta
     9421 tgccctcagt gtgcaaggcg aaccggcaaa aggcgaaatg cttgcgggca cggccgtgta
     9481 caacggcgaa gtgctgcatt tccatatgga aaacggccgt ccgtccctgt ccggaggcag
     9541 gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcggcga
     9601 tgatttgcat atgggtacgc aaaaattcaa agccgttatc gatggaaacg gctttaaggg
     9661 gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga
     9721 agaagtggcg ggaaaataca gctatcgccc gacagatgcg gaaaagggcg gattcggcgt
     9781 gtttgccggc aaaaaagagc aggattgatg ctgatgccgc ctgaagcggg ggcgttcaga
     9841 cggcattgtg atgtttgggg tggggtccgg ttcaagtccg catcagttga taaacccgtc
     9901 gtctttcatt ttcttttcgg aaaaatactg gccgaatacc agcagggcag cgaggatgat
     9961 gacaaacagt cccgccatag tcagagtgat tttgaaaaac aattccgctt tgagaaaaga
    10021 aaaccagagt tgcacaatta gcagcagcac ccagccggca aacagtccga gacaggcata
    10081 aacgccgagc ttaaacacga tcccgctcca aaatgacgac gtaggcctcg cgtttgaaaa
    10141 acagcagcat acggcgcaaa tctttttcca tcgttacaac ccgccagcct tcgggggttt
    10201 gtttattgag gaactcgctg aatttgatgg ggttgacctt tgccgcgccg aaaaacaggc
    10261 tggacaacag gctttcctga taaatgatga ctttgtattc tttcatcgta tttccttttc
    10321 ggtttaaacc ccgcccttta aggcggtaga attagacttt atttgggagg ggcgcaaccc
    10381 cttccaaatc aggacgacac atagggcggt gctttatgtg ccgccctgtg tgttgaaaca
    10441 taggattcct ttaattgagg gattgccttt tcagacaatc ccttaaattg cagcgatgca
    10501 atgtggtgaa acttattgct tattacccaa ctgcggcaat accgaacctt cgcccaatgc
    10561 caacaaaccg ctttgcgagt aaatgcccag tttggcgcgg gtgtcggtaa tgtcgaggtt
    10621 gcgcatagtc agttgtccga tacggtcgag cggtgtgaac gctgcgtctt cgactttttc
    10681 catactcagg cgttcgggtt ggtaggtcag gttgggcgat tcggtgttca gaatcgagta
    10741 gtcgttgccg cgacgcaatt cgagggttac ttcgccggta atggctttgg caacccagcg
    10801 ttgggcggtt tcgcgcaaca tcagggcttg gctgtcgaac cagcggcctt ggtagagcaa
    10861 acgtccgagg cgcaggccgt tgatgcggta ttgttcgatg gtgtcttcgt tgtggatacc
    10921 ggtcaccaaa cgctcgtagg cgatgtggaa caacgccatt cccggggctt cgtagatgcc
    10981 gcgcgatttg gcttcgatga tgcggttttc gatttggtcg ctcataccca gaccgtggcg
    11041 gccgccgatg cggttggctt cgaggaagag ttcgacggga tcagcgtatt ctttgccgtt
    11101 cagtgcaacc ggcacgcctt cttcaaagcg cacgctgact tcttcgggtt tgatttcgac
    11161 gttttcgtcc cagaaggcaa tgcccataat aggtttgacg attttgatgc ccgagttcaa
    11221 aaattccaaa tctttggctt cgtgggtcgc gccgagcata ttggaatcgg tggaataggc
    11281 tttttccacc gacattttgt agttgaaacc gttggcaatc aaaaattcgc tcatttcatg
    11341 acggccgccg agttcgtcga taaattgctg gtcgagccag ggtttgtaga ttttcagcgc
    11401 gggattggtg agcaaaccgt agcggtagaa acgctcgatg tcgttgcctt tgtaggtgct
    11461 gccgtcgccc caaatattca catcgtcttc tttcatggcg gaaacaagca tagtgccggt
    11521 tacggcgcga cccagaggcg tggtgttgaa gtaggcgatg ccgccggtgg aaacgtggaa
    11581 cgcgccgcat tggatggcgg cgatgccttc gtgtgccagc tgtgcgcggc agtcaatcag
    11641 gcgggcgttt tccgcaccgt attccatcgc ttttttggga atggcgttgt agtcgtcttc
    11701 atcgggctga ccgaggttgg cagtgtaggc ataaggcagc gcgcctttga gtttcatcca
    11761 caacagcgcg gcggaggtat caagtccgcc ggagaaggcg atgccgactt tctgaccgac
    11821 gggaaggttt tgtaaaatag tatggttttg gttcatgaaa aaatcctcga tagcttgaaa
    11881 aatcaaagcc attatcttat aaataggaaa tattgtcagc catcatgccg tctgaacggg
    11941 tattccgccg tttcaggcgg tatttaaaac cgaaaacatt atgaacgcgt tcaaccttat
    12001 catcatcggc gacgaaatct tacacggcag ccgccaagac aagcatttcg tctttttcaa
    12061 atccctgctc gaatccaaag ggctgaagct caatcaggtg caatacctgc ccgacgaacc
    12121 tgatttgttg gtcaaacaac tgcgccgcag cttttcagac ggcatgccaa ctttcgttac
    12181 cggcggtatc ggcgccacgc ccgacgacca cacccgccaa gccgcagccg ctgctttgga
    12241 tttgcccgtc gtccgccacg cgcaagccgc cgggtttatc gaaggcgtga cccttaaacg
    12301 cggtgagccg ctcgattcgc ccgaacacgc ccaacgcctg aagatggcgg attttcccca
    12361 aggcgcggaa ttggtgccta atccgtttaa taatatcgcc ggattttcca ttcgcgagca
    12421 ttatttcttc cctggttttc ctgtgatggc gcacccgatg gcggaatggg ttttggatac
    12481 ttattacgcc ggccgcttca accaaaccca acgcggcagc cgcagcgtgt atgtgttcga
    12541 gcagcccgaa tcgcgcatta cgccgattat ggagcatatc gagcaaactt atctcggggt
    12601 gcgttcgtac agcctgccca gcgtcggttg gacgcattca gacggcacgc aggtcaaacc
    12661 gcacattgag ttcggcatca aagccgaagg agaagccgtc aaccttttgg acgcggcgtg
    12721 ggcggaagtg ttgcacagct tggaaggatt gggagcagag ctgaaaaatc gggtaaactg
    12781 aaaaacgttt gcccaaatcg gacgatattt aaggaaaacc atgcaatacc gaatcagaag
    12841 agaaaacgaa gcacccgaag caaaaaatgc gggggaaacc ttatgggagc gcgatatgat
    12901 gcgcgaagtg ctgctgtccg cctatcggga caggcgcagg gagcggatgt ggaaaaacat
    12961 ctggcgcgcc gtcagcaccc tgattctggt tgccctgatt gcaggcattt tccgaaaaga
    13021 cgaagccgca ttgcagttgg cgggcaatac gccgcatacc gcagtcgtca acctgtacgg
    13081 cgaaatcggc aacggtgtag aagaccaggt caaaaaactc aaagacggta tggaggccgc
    13141 ctacaaaaac ccgcaggcaa aagccatcgt catccgcgcc aacagccccg gcggttcgcc
    13201 cgtcgtctcc aacaccgctt ttgaagaaat acgccgtctg aaggcgcagc accccggcat
    13261 tcccgtttat ctcgtggcgg aagatatgtg cgcgtccggc tgctactaca tcgcggcggc
    13321 ggcggacaaa atctatgccg acccgtccag catcgtcggc agcatcggcg ttatcggcag
    13381 cagtttcgac gcgaccggcc tgatggaaaa aatcggcgtg aaacgcaggg ttaaaattgc
    13441 gggcagcaac aaaggcatgg gcgatccgtt ttcgcccgaa acgcccgaac agtcgaaaat
    13501 ctgggaagaa atgctgaccg gcatacacgg cgaattcatc aaagccgtca aaaccggacg
    13561 gggcggccgc ctcaaattcc gacaatatcc cgacgtgttc agcggtcggg tgtacacggg
    13621 tgcggatgcg ctgaaagtcg ggctggtgga cggactcggc aatatttaca gcgtggcgcg
    13681 agacgtggtc aaagcccccg atgtggtgga ttacactccg aaggacgatt tcggcagaat
    13741 cttgggcaga cgcttcggcg cagaactcaa agcaagcgtg cgcgaagccc tgcaggccgt
    13801 ccggtagcgt tgtttacagt atttctaagg gaataccaaa ataaaaatat acacaaaaac
    13861 actcacctcc ccagtatgct gccactaagt cagccgcgtc actatttacc gtgcacttaa
    13921 agccgtaaaa ggcaggttgt ccccgggagg ttttgtgcat cctgataccg atcggttgtt
    13981 gttgctcaaa ggacagaagg ccgctgataa acgagattac ctgtttgtcg ctattgacga
    14041 tttttatact ctgccatttt gccagacaaa accgcagaca gtgctgccaa gtttctgacc
    14101 gaacatctgg ccgacccctg cttgtacctg attgagtacg tttactctga caatgatagg
    14161 taatataaag agccgtccaa catgctttcg gtgcagtttg ttatgataat gggattggtt
    14221 ggaggcttgc ccgatttgct tgtccgcaga ccaacggtaa ggcggagcgg gttatccgta
    14281 ccttgatgga gatgtggcat gaggaacagt cgtttgacag tccggaacat cggcaaaagg
    14341 agttgtgctg ctttattaat ttaggggctg ccctagatca ctagggaaat tcaaattaag
    14401 ttagaattat ccctatgaga gaaagtcgtc taagccggta taaacaaaat aaactcattt
    14461 tgttatcgtg gctatgatgt attggatgcg ggcgaattta gccatttccg tatcaatcac
    14521 agcacacatt ttgccgaacg acaaaaccat attaatggaa
//
LOCUS       DAWKQB010000042        14014 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.042, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000042 DAWKQB010000000
VERSION     DAWKQB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14014
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.042"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(226..549)
                     /locus_tag="WJL14_000919"
     CDS             complement(226..549)
                     /locus_tag="WJL14_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ7656010.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAVEVDA
                     EMARY"
     gene            complement(629..1285)
                     /locus_tag="WJL14_000920"
     CDS             complement(629..1285)
                     /locus_tag="WJL14_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ7656011.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1387..1890)
                     /locus_tag="WJL14_000921"
     CDS             complement(1387..1890)
                     /locus_tag="WJL14_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ7656012.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            2074..2847
                     /locus_tag="WJL14_000922"
     CDS             2074..2847
                     /locus_tag="WJL14_000922"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ7656013.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WJL14_000923"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WJL14_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ7656014.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WJL14_000924"
     tRNA            3218..3303
                     /locus_tag="WJL14_000924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4103..4471
                     /locus_tag="WJL14_000925"
     CDS             4103..4471
                     /locus_tag="WJL14_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ7656015.1"
                     /translation="MRIFKNQWIVKFAKKHKINDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4468..4779
                     /locus_tag="WJL14_000926"
     CDS             4468..4779
                     /locus_tag="WJL14_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ7656016.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            4889..5100
                     /locus_tag="WJL14_000927"
                     /pseudo
     CDS             4889..5100
                     /locus_tag="WJL14_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692461.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5161..6090)
                     /locus_tag="WJL14_000928"
     CDS             complement(5161..6090)
                     /locus_tag="WJL14_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7656017.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFKRILCLSA
                     VISVLGACTVVADVYGQDSATMNAAAAEDYMKTVELNKSAGNVDTTSKTARRVQAVFR
                     RMLPYADAANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDGEIAAI
                     MGHEMTHALHEHGKNKVGQKILTNMAAQIGTQIILDKKPDTNPELVGLGMDILGMYGI
                     TLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTHPTNN
                     ARIENLKRLLPTVMPVYEHSVRNKGRVNKNRRR"
     gene            complement(6113..6244)
                     /locus_tag="WJL14_000929"
     CDS             complement(6113..6244)
                     /locus_tag="WJL14_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ7656018.1"
                     /translation="MRDFDKLAEEAAEHAAEGAVKFIDGKPAKKIIAVPGRLANIVV"
     gene            complement(6374..7165)
                     /locus_tag="WJL14_000930"
     CDS             complement(6374..7165)
                     /locus_tag="WJL14_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ7656019.1"
                     /translation="MNLFFDTKLGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMQR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNSVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIRDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTLKAF"
     gene            complement(7395..10127)
                     /locus_tag="WJL14_000931"
     CDS             complement(7395..10127)
                     /locus_tag="WJL14_000931"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ7656020.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYSLPKKQVLQRVTIGLSPEQEEAMGFGGGSGC
                     GAGYGDGSGSDSRDLPFMPNKWQAWYGVKDDFMVLINSGELDGMNYQTAFDAVAAKLQ
                     AIGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEQCGDVPVPADQLPVVLPENVVPDG
                     MGSPLAKMPEFYETTCPCCGGAAKRETDTMDTFMESSWYFFRYMSPKFSDGMVDPAAA
                     KYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYR
                     ENDKGGKDWINPADVELTFDDKGRPVSAVLKVDGLPVVISGTEKMSKSKNNGVDPQEL
                     INAYGADTARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGEAVNAFA
                     GSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGSEQG
                     RTVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPAVDEAALVKSEIEVMVQ
                     VNGKLRGKITVAADASKADLEAAALATEGAVKFMEGKPAKKIIVVPGRLVNIVV"
     gene            10397..10912
                     /locus_tag="WJL14_000932"
     CDS             10397..10912
                     /locus_tag="WJL14_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7656021.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            10919..11362
                     /locus_tag="WJL14_000933"
     CDS             10919..11362
                     /locus_tag="WJL14_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ7656022.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11531..13588
                     /gene="ppk1"
                     /locus_tag="WJL14_000934"
     CDS             11531..13588
                     /gene="ppk1"
                     /locus_tag="WJL14_000934"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ7656023.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGAQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            13805..>14014
                     /locus_tag="WJL14_000935"
     CDS             13805..>14014
                     /locus_tag="WJL14_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7656024.1"
                     /translation="MLPWEYEDNTLQIKEKSTERTDPFFVLEVTARSAADILGIHPNS
                     AVLFYRKIRTVINHHLALAADEVFEG"
BASE COUNT         3480 a         3966 c         3421 g         3147 t
ORIGIN      
        1 cagcctgcac attgaaaatg ccgcccaagc aaactttcag tttgccagcc tcgtcctagc
       61 cctctcccac ggggagaggg gattggggtg caggctgcct ttaaggttca ggcaaatttt
      121 aacttcgttg ataccgcgct ttagcttcgc agaagctgca ctttcagacg accttttgga
      181 acaccacagg tacacgcatt taaggaatgc cgtctgaagc cgccttcaat aacgcgccat
      241 ttccgcgtca acctcaaccg cccacgcgtc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt gatggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcacccccca aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaaccc
      601 gctttcagac ggcatcgcaa tccgaacatc aaaactgaaa accgccaaac ggatcggcat
      661 ccttatcatc caaatgcgcc accaaggtat cgaacagcac cttctcttca aacacatcgc
      721 ccttgcgcgt aatcaaaagc gcgcgctgaa caggcttgcg gcctacgata accaccatgc
      781 gtccgccatc tttcaactgt tctttcaaca cttcaggcac aaggtttacc gcaccgccga
      841 cataaaccgc atcaaacggc gcacctgcgg aaagttcggt caacccgttg ttttgcacat
      901 aatcaatgtt ttccaaagac aagccgtcca acaccgcttt ggcgcggttt tgctgttcgg
      961 catcgatgtc gtccgacacc acacgaccag ccaattttgc caaaagcgcg gtcgcatagc
     1021 ccgaacccgt gccgatttcc aaaaccgtat cgtttttcgt cagcttcaag ccctgcgcca
     1081 gccgcgccac gactttcggc tccagcatct tatgaccgtt ggcaagcggc agcgccatat
     1141 ccgcatacgc caaaccctgc aaatcctcat cgacaaaaag ctcgcgcgga atctccgcca
     1201 aagcgtccaa aacgtcaaaa tccaatacat cccacggacg gatttgctgt tcgaccatat
     1261 tgaaccgcgc tttttcaaaa tccatttagt gctccgtcaa ataattgttt caacaatggc
     1321 aggcattata aaaccacact cccgccgcgc caacttcggc acgcgccgcc cgcccctttc
     1381 catcactcaa gccgcctggc gcgaagcctc aaaccgcttc tccaaaatct tcgccagttc
     1441 gcccaaatac aaagcatctg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gcgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtc cacatccccg acgaccaccg tcccgccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgtctcgtcc gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccgaaaca acattccaaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc accaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccagaacaa caattcgctc
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc gacaacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctgcaagggc tggataccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcgatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac tttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgc tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacga caaaacacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccgtaa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gcctgatgcc
     3361 atcaaccaat cccaatcaat acaaggcttt caggctgatt gttatcctgc cgccccccct
     3421 tcctgaccgt acaatcccgt ccaatccgcc ctaattgaac taacctgatt ttttacggca
     3481 tccgaaaata ccgcgaaaaa tacgtgaaaa taaagctgaa cgaccaccaa atcaggaatg
     3541 ccaagcggga aatagcttgc ggggaatact gacgcaccgt agggaatgag ggggaaaccg
     3601 tccaagatgc agagcggttt ttttgggttt ttggaaaaaa cctatactat agtggattaa
     3661 atttaaactg gtacggcgtt gcctcgcctt gccgtaccgg tttaaattta attcactata
     3721 aaataagaaa aagataaaaa attggtaaca aatgcggtaa caaatggtaa cgaatcggta
     3781 acaacttttg gggttttccg gtttttcacc gtcttggcag tgggagcgta gcggaatgaa
     3841 aagccaaaac gcacggaacc gcgcctattt tgagcaggaa tggcggttaa accacttggt
     3901 tatatatggg ggcataggaa gacagcgaaa cgcgctaaaa acgcaatttg agccattaaa
     3961 agcgattgat taaaaaataa tcaggttagc cgccagtgtt tcaggcggca taaacggaga
     4021 aattgcgggg cataaaaaag gcagcttgcc gtgttgtctg tctctggtat aattccaagt
     4081 atcactaatc aacggctaca caatgcggat attcaaaaac caatggatag tgaaatttgc
     4141 caagaagcac aaaatcaacg attccgagct gctggaagcg gtagagcggg cggataacgg
     4201 gctgatagac gcagatttag gcggcggtgt gattaagcag cgcatagcaa ggcaaggaca
     4261 aggcagaagc ggcggctatc gcagcctgat attgttcaaa caggcagata gggcattttt
     4321 cgtttacgcc tttgccaaga acgacaggga aaacatttcg gataaagaac tggacgttta
     4381 ccgaaaagcc gccgcatatt atctgaaata cacgcgggca gagctggcgg ctttgaaaga
     4441 agacggcatt atcacggaga tagaatcatg aaatacaaaa gcgaggcatt agccgccatt
     4501 catgaaatga tggaaggggc ttacaacatc ggcgcaatcg acaaaaagac tatgcgcggc
     4561 tttgacaagt cctgcctgac cgaaatcaaa ccgttgagcg gcggagacat caaggcaatc
     4621 agggagaagg aggcactatc gcaagccgct ttcgccatct atctcaacgt gggaaaaaat
     4681 cacgtttcgg cttgggagcg gggcgttaaa aagccgagcg gcgcggcgtt gaagctgctg
     4741 accatcgtca aaaacaaggg catcgaagcc attgcgtaac cggcttggca aacggcaaaa
     4801 tcagcaagtt cacaatagac gcgctgctga atatgcctga cgaaacaggc aagaccgccg
     4861 aactgaatat cagggcgtag ccgcacaaat gcccgaccgc atcaaaccaa gccgaaacgg
     4921 cggcggtgca gacgacatag cccgacagca aggcacggcg cagacgggcg cgaaacccga
     4981 aacatccccg accgcgaggc acggggattt ttttgcgccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaagg caaaacgggc ggggctcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga ctcccttccc
     5161 ttaacgccga cggtttttat taacgcgccc cttatttcta acactgtgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgtttg tcggatgagt
     5281 agaggtaata gcataaataa agccgttttg gtcgttttcc tgattcattt tttcccaaac
     5341 cctgacagcg gctgccggat gatagcctgc ctgcgccatc aacatcattc ccccctcatc
     5401 ggcttcttct tccaagctgc ggctataagg caaggtaatg ccgtacatcc ccaaaatatc
     5461 catacccaat ccgaccaatt ccggattagt gtccggtttt ttgtctaata taatctgcgt
     5521 gcctatctgc gccgccatat tagtcaagat tttctgcccg accttatttt taccgtgttc
     5581 atgcagggca tgcgtcattt catgccccat aatggcggca atttcgccat cggtcagctt
     5641 aagtttatcg actatccccg tataaaacgc cattttcccg ccgggcattg cccacgcgtt
     5701 cagctcatcg tttttgaaaa ccgtcatttt ccagtcaaac ttatggccgg tattatttgc
     5761 cgcatcggca taaggcaaca tacgtcgaaa tactgcctgc accctacggg ctgttttgga
     5821 tgtagtatcg acattgccgg cagacttgtt caactcaacc gttttcatat aatcttcggc
     5881 agccgcagca ttcattgtgg cggaatcctg accgtaaaca tcagcaacga ccgtacaagc
     5941 ccccaatacc gagattactg ccgacaggca gagtatccgt ttaaaggaag gaaggaagga
     6001 aatttcatat ttaggtttac tccttaaaaa attaaatttc aaaaaaatgc cgtctgaatc
     6061 caaaacggat ttcggacggc atcttaacat tgtttaatgt ttttaaaaag atttacacca
     6121 cgatgtttgc cagtctgccc ggtacggcga tgattttctt ggcaggcttg ccgtctatga
     6181 atttcaccgc gccttcagcg gcgtgttcgg cagcttcttc agccaatttg tcgaaatcac
     6241 gcataaattg ccaataattc tccgactttc ttacgactac tacctttggc ggcaatattg
     6301 cgctgtactt caactgtttt cagaatgaca gaagaataaa tatcccttgt gaattcagta
     6361 tcatgatttg aaatcaaaac gccttgagag tcggacgcaa tttgtcgatt tttttgtaaa
     6421 gtccgcgacc aatgaattcg atcgtatttt ggtcgcgcag aatttgcaac tgttggcgga
     6481 ttttgtctct gatatggttg ttttggggaa attggatgga tagtttgttt tcaaattcat
     6541 acatttgcga caatgtgaat tcttcgggga gttggtcgat acatttcata acagccagaa
     6601 gccagccttt gcgctccgca ttttggttgc gtaaaaacag gttggattgc cattttttca
     6661 gaacggtttc gggttcgata atgcgggaat cgtctattaa gaatattttt ccgctttcag
     6721 gcaaaggggc gagattgatg gaacacataa tatggtgcgg ccggttttta atgcctttat
     6781 ttctgggaat aatcatatcc ggcgtgatga aatgtttggg tacaagcacc aattgccgta
     6841 tggagtaatc cgctttttta tatgcaagaa agaaaaagtt ggggttggta tctgaccgga
     6901 tgcgctccaa catggtgtga tatgcaccgt caggcacgct gttgcctatg gttttttgat
     6961 ttttactctt taattcatat tgctcgtggc aatttgggca aaagaggtct gcaacaggtt
     7021 tgttattggc aaatctctgc atcggcttgc ttccgcaaca gggacagtag ccgttttttt
     7081 ccagccaagc ctcgctcatt acacggattt tatgggttgc tttattttgt tgctttccca
     7141 atttggtatc gaaaaataaa ttcatgtttt ggattttgag atttcagttt tagtggatta
     7201 acaaaaatca ggacaaggcg acgaagccgc agacagcagg gcaaggcggg gcaacgtcgt
     7261 accggtttaa agttaatcca ctatatttga ggtctgtaat gcagacaact ccatctgcct
     7321 taaaaaggcc gtctgaaacc ctgtttccaa gtttcagacg gcctttatcc gtatggctaa
     7381 accttaaaag cggcttagac gacgatattc accaagcgtc cgggaacgac gatgattttc
     7441 ttcgcgggct tgccttccat gaatttcacc gcgccttcgg tggcgagtgc ggcggcttcg
     7501 aggtcagctt tggaggcgtc ggcggcgacg gtgattttgc cgcgcagttt gccgttgact
     7561 tgaaccatca cttcgatttc ggatttgacc aaggcggctt cgtcgactgc cggccagcca
     7621 gcttcccaca gtttcgcgcc gttcaattcg ctccacaggg tttcgcagat gtgcggcacg
     7681 atgggccaca gcaggcgtac ggcagcttcc aatacttctt gggcgacggt gcggccttgt
     7741 tcgctgccgg tgtcggtttt gtcgtattgg ttgagcagtt ccatcacggc ggcgatggca
     7801 gtgttgaact gctggcggcg gccgtagtcg tcgctgactt tggcgatggt ggaatgcagt
     7861 ttgtggcgaa ggtctttgag ttctttagac aaaccgtctt ggctgcctgc gaacgcgttg
     7921 actgcttcgc cttgcttcaa gtattcgtaa acggtacgcc acaggcggcg caggaagcgg
     7981 tgcgcgcctt cgacgccgct gtcgctccat tcgagggact gttcgggcgg tgcggcgaac
     8041 atcatgaaca ggcgggcggt gtctgcgccg taggcgttaa tcagttcttg cggatcgacg
     8101 ccgttgtttt tggacttgga cattttttcc gtgccgctga tgacgacggg cagtccgtcg
     8161 actttcagga cggcggaaac agggcggcct ttgtcgtcaa aagtcagctc gacatcggcg
     8221 gggttgatcc aatctttgcc gcctttgtcg ttttcgcggt agtaggtttc gcaaacgacc
     8281 atgccttgcg tcagcaggcg ttcaaacggt tcgtcaacat tgaccaaacc ttcgtcgcgc
     8341 atcagtttgg tgaagaaacg cgcgtacaag aggtgcaaaa tcgcgtgttc gatgccgccg
     8401 atgtattggt cgaccgcgcc ccagtatttc gcggcggcag gatcgaccat gccgtctgaa
     8461 aattttggcg acatgtagcg gaagaagtac cagcttgatt ccatgaaggt gtccatggtg
     8521 tcggtttcgc gttttgccgc gccgccgcag catgggcagg tggtttcgta aaactcgggc
     8581 atttttgcca gcggcgaacc catgccgtcg ggtacgacgt tttcaggcaa aacgaccggc
     8641 aattggtcgg cagggacggg tacgtcgccg cattgttcgc aatggacgat gggaatcggg
     8701 cagccccagt agcgttggcg cgaaatgccc cagtcgcgca ggcggtattg ggttttcggt
     8761 tcgccagctc cgatagcttg taatttagcg gcgacagcat caaatgctgt ctgataattc
     8821 atgccgtcca gttcacctga gttgattaag accatgaagt catctttgac tccataccat
     8881 gcttgccatt tattaggcat aaacggcaaa tctcttgaat cggaaccact accatcacca
     8941 taaccagcac cacatccgga accgccacca aaacccatag cttcttcttg ctctggcgaa
     9001 agtccgattg ttacgcgttg taagacctgt tttttcggca ggctgtattt ggcggcgaac
     9061 tcgaaatcgc gttcgtcgtg cgccggaacc gccattaccg cgccgtcgcc gtagccccac
     9121 aatacatagt tggcaatcca cacttccagc ttgtcgccgt tgagcgggtt gacgacgtag
     9181 cggccggtcg gcacgccttt tttctccatc gtcgccatat cagcttcggc aaccgaaccg
     9241 gctttgcatt cggcaataaa tgcctgcaat tcgggtttgt cggcggctgc ggcggttgcc
     9301 agcggatgct cggcggcaac ggcaacataa gtcgcgccca tcagcgtgtc gggacgggtg
     9361 gtataaactt gcaggaattt cgcgtaatcg ccttccaagc cttgtttgct gtcgtctgaa
     9421 acggcgaagc gcacggtcat accgcgagat ttaccgatcc agttgcgctg catggttttg
     9481 acttgttccg gccagtgttc cagcttgtcc aagtcgttga gcagttcttc ggcgtaatcc
     9541 gtgattttga agtaatacat cgggatttcg cgtttttcga tcaatgcgcc ggaacgccag
     9601 ccgcgtccgt cgataacttg ctcgttggca aggacggttt ggtcgaccgg gtcccagttt
     9661 accgtgccgt ttttgcgata gacgatgcct ttttcaaaca gcttggtaaa cagccattgt
     9721 tcccagcggt agtattcggg tttgcaggtt gcggtttcgc gcgcccagtc aatcgcaaaa
     9781 cccaggcttt tgagctgggt tttcatgtat tcgatgttat cgtacgtcca agcggcaggg
     9841 gcgacgttgt ttttcatcgc cgcgttttcc gccggcatgc cgaacgcgtc ccaacccata
     9901 ggctgcatga cgttgaagcc gtttaaaagt ttgaagcggc tcaatacatc gccgatggtg
     9961 tagttgcgca catgccccat atgcagcttg ccgctgggat aggggaacat ggagaggcaa
    10021 taatatttgg gtttggaagc gtcttcggag acgttgaaaa tacgggcgtc gtcccatttt
    10081 ttctgcgccg caggctcgat ggcggcgggt tgatattgtt cttgcatagt cattctgttt
    10141 tcgcttaaaa acgttggaaa aataaagtcg gcatcaatta taacaggttg ccggaagcgg
    10201 cgaatcggca gattgccggc aggatgcgta aattcgcacg cgcattattc cgtatgccgt
    10261 acaaatacac cgcgtttatt gatacgcacg ttttttatgc taatattaca aaccaaaatc
    10321 aaatgtttaa aactctcctg atgcggctct tccgaacaaa aggcagacgg gcatcgggta
    10381 aaagaggatt ctgcatatga aaatcaaaca aatcgtcaaa ccgggcttgg cagtattggc
    10441 ggcgggcgtt ctgtctgcct gcgcaaccaa aagcaacgtc aaagccgacg gcacgaccga
    10501 caatccggtt ttcccgaaac cctattccgt aacgctcgac aacaagcgcg gcacattccc
    10561 gacttatgac gaactggatc agatgcgccc cggcctgacc aaagacgaca tctacaaaat
    10621 cctgggccgc ccgcattacg acgaaggtat gtacggcgtg cgcgaatggg attacctgtt
    10681 ccacttccat accccgggcg taggtatcga ccctgaaaac acttccggcg tagaagatgt
    10741 tactacctgc caatacaaag tgattttcga taaagacaaa tttgcccgca gcttctactg
    10801 gaaccccgtc ttcccgaaag atgccgcctg tccgccgccc gcacccaaag ccgagccgca
    10861 agtcatcatc cgcgaaatcg tgccggcaaa accgaaacgt atccgccaat aatccgacat
    10921 gccgttccgc ctgtttttag ggatattatg cggcctgtca atggttgccc ccgtatatgc
    10981 acaggggcaa ccggatgcgg tcaacgctta tattcaaaag aaaaaagtca tcgtaaacac
    11041 atccaaagcg gaactctgtt ttgccgatga caggcagtgc caccccgtcc tcatcggtgt
    11101 tgccacgccc aaggggacgt tcgggctgac gctgaacagt accgacaagc ctggatacgg
    11161 cggcgaagtc atcggtttca agcaggaggg tgattttctt ttcgccctgc accgcgtttg
    11221 gaatcagata ccgtcggaaa ggcggaacga acgcatcgcc tccccgtccg tgtccgacag
    11281 gattatgacc aacggctgca tcaacgtcag cgatgcggtg tacgaaaaac tgcgtcatta
    11341 ttttgtgttg gaagtgattt gaaacagacg gataccgcac gcgccggtat ctgttttcac
    11401 attgccccga tgcctgaaac aggctgtccg ccacgtcatg ccgtctgaaa ccggcgcaga
    11461 tgccgccaag ccttcagacg gcattgcctg cccgctccga ccgaacaaca accatctttg
    11521 ggagaacctt atgcccgaac aaaaccgcat cctctgccgc gaactaagcc tgctggcatt
    11581 caaccgccgc gtgttggcac aggcggaaga caaaaacgtc ccccttttgg aacgcctgcg
    11641 cttcctgtgc atcgtgtcgt ccaatcttga cgagtttttc gaagtccgca tggcgtggct
    11701 caagcgtgaa aacaagctgc acccccggcg caggctggac aacggcaaaa tgccgtctga
    11761 aaccatcgcc gacgttaccg aagcggcgcg ctccctgata cggcaccagt acgacctgtt
    11821 caacaacgtc cttcagcccg agttggcgcg ggaaagcatc catttttacc gccgccgcaa
    11881 ctggacgggc gcgcagaaaa aatggatcga agactatttc gaccgcgaac tgctgccgat
    11941 cctgaccccc atcggactcg acccgtccca ccccttcccg cgaccgctga acaaatcgct
    12001 caacttcgcc gtcgaactcg acggcacgga cgcattcggc aggccttcgg ggatggcgat
    12061 tgtgcaggca ccgcgcatcc tgccgcgcgt cgttcccctg ccgtccgaac tgtgtggcgg
    12121 cggacacggc ttcgtcttcc tgtcgtccat cctccacgcc cacgtcggca aactcttccc
    12181 gggcatgaac gtcaaaggct gccaccagtt ccgcctgacg cgcgacagcg acttgaccgt
    12241 tgacgaagaa gacctgcaaa acctccgcgc cgccatccaa aacgaactgc acgaccgcga
    12301 atacggcgac ggcgtgcggc tcgaagtcgc cgacacctgt cccgcctaca tccgcgactt
    12361 tctgctcgcg caattcaaac tgaccgccgc cgaactctat caggtcaaag gcccggtcaa
    12421 cctcgtgcgc ctcaacgccg tccccgacct agtcaaccgc cccgatttga aatttcccac
    12481 acacacgccg ggcagactga aagccttggg caaaaccgcg tccatattcg atttggtgcg
    12541 ccaatcgccc atcctgctgc accaccccta ccaatcgttc gaccccgttg tcgaaatgat
    12601 gcgcgaagct gccgccgacc ccgacgtgct tgccgtcaaa atgacgattt accgcaccgg
    12661 cacgcgttcc gaactcgtcc gcgccctgat gaaggcggca ctcgccggca aacaagtaac
    12721 cgtcgtcgtc gaactgatgg cgcgttttga cgaagccaac aacgtcaact gggcgaagca
    12781 gctcgaagag gcgggcgcgc acgtcgtgta cggcgtgttc ggctacaaag tccacgccaa
    12841 aatggcactg gtcatccgcc gcgaagacgg cgtactcaag cgttacgccc acctcggcac
    12901 gggtaactac caccaaggca catcgcgcat ctacaccgac ttcggcatca ttaccgccga
    12961 cgaacaaatc accgccgatg tgaacacatt gtttatggaa atcacaggct tgggcaaacc
    13021 cgggcggctg aacaaactct accaaagccc gtttaccctg cacaaaatgg ttatcgaccg
    13081 catcgcacgc gaaaccgaac acgcaaaagc cggcaaaccg gcgcggatta ccgccaagat
    13141 gaattcgctc atcgaaccga ccgtcatcga agccctgtat cgggcaagcg cggcaggcgt
    13201 acaaatcgat ttgattgtgc gcggtatgtg caccttgcgc ccgggtgtaa aaggcttgtc
    13261 cgaaaacatc cgcgtccgct ccatcatcgg caggcagctc gaacacgcgc gcgtgtattg
    13321 cttccacaac aacggcgcgg acgatacctt tatctccagc gcggattgga tggggcgcaa
    13381 cttcttccgc cgcatcgaaa ccgccgcgcc gattaccgcg cccgaactca aaaaacgcgt
    13441 catccgcgaa ggtttggaaa tggcattggc agacaatacg cacgcgtggc tgatgcagcc
    13501 cgacggcggc tatatccgcg ccgcgcccgc cgagggcgaa tccgaagccg acctgcaaaa
    13561 cgatttgtgg acactgctcg gaggctgacc cgcaccgccc caatcaaaaa ccatgccgtc
    13621 tgaaaccttt ccgtttcaga cggcatagtt ttacagcaat ctaaacaggg cggaccggag
    13681 tcaaaaacac accttcgcca ttcctgcaca agcacttccc ctatacgctc ccaaccccaa
    13741 gccgccgcat tccagacggc attatagtgg attaaatttt aggggctgta ctagattagc
    13801 agatatgtta ccctgggaat atgaagataa cacactgcaa attaaagaaa aaagtacaga
    13861 aagaactgat ccgttttttg tactcgaagt taccgcccgt tctgccgccg atattttggg
    13921 tatccatccc aattcggcag tactgttcta ccgtaaaatc cgcacggtta tcaaccatca
    13981 tttggccttg gctgccgatg aggtttttga gggc
//
LOCUS       DAWKQB010000043        13748 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.043, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000043 DAWKQB010000000
VERSION     DAWKQB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.043"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..2045)
                     /locus_tag="WJL14_000936"
     CDS             complement(69..2045)
                     /locus_tag="WJL14_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ7656025.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WJL14_000937"
     CDS             2194..3033
                     /locus_tag="WJL14_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ7656026.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="WJL14_000938"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="WJL14_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7656027.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4417..5142)
                     /locus_tag="WJL14_000939"
     CDS             complement(4417..5142)
                     /locus_tag="WJL14_000939"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ7656028.1"
                     /translation="MRIPLLAPDNYAFPDPTYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(5212..5646)
                     /gene="fur"
                     /locus_tag="WJL14_000940"
     CDS             complement(5212..5646)
                     /gene="fur"
                     /locus_tag="WJL14_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ7656029.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHSEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5865..6242
                     /locus_tag="WJL14_000941"
     CDS             5865..6242
                     /locus_tag="WJL14_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ7656030.1"
                     /translation="MNKTLILALSALLGLAACSAERASLFPSYKLKVIQGNEIDPRAA
                     AALRLGMTKDQVLLLLGSPLLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RTEGDALQNAAEALKDRQNTDKP"
     gene            6252..7061
                     /gene="dapB"
                     /locus_tag="WJL14_000942"
     CDS             6252..7061
                     /gene="dapB"
                     /locus_tag="WJL14_000942"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ7656031.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAIRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7175..7585)
                     /locus_tag="WJL14_000943"
     CDS             complement(7175..7585)
                     /locus_tag="WJL14_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656032.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFVYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LELIGGRYDFPTAFRIDIVCREIELEFLDQEGFN"
     gene            complement(7587..7833)
                     /locus_tag="WJL14_000944"
                     /pseudo
     CDS             complement(7587..7833)
                     /locus_tag="WJL14_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7841..8995)
                     /gene="lpxB"
                     /locus_tag="WJL14_000945"
     CDS             complement(7841..8995)
                     /gene="lpxB"
                     /locus_tag="WJL14_000945"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ7656033.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVKRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRAGIPTLHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATAATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQHDAV
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9061..10053)
                     /locus_tag="WJL14_000946"
     CDS             complement(9061..10053)
                     /locus_tag="WJL14_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ7656034.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            10547..13306
                     /locus_tag="WJL14_000947"
     CDS             10547..13306
                     /locus_tag="WJL14_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ7656035.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKHNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         3462 a         4476 c         3204 g         2606 t
ORIGIN      
        1 tgaattttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggcctgtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccatttt cgccgaatcc tgcctcatca tcactaccga caaacaaacc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccggta cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgccccaaac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagcaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccttgtgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaatac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccaatgc tgaacatcag gaacacaatc
     1861 ccgatttcac ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcatagacc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacac cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt cattttttgg cagtgatttt gcccggctgt cgccatttcc aattttaatc
     4381 caccatattt gaacctgacc ggagcataag ccgccatcaa agccccttgc acgccacttc
     4441 gcgccgcccg atttcctctt tcaacggcac ggcgttcaac atccgcagac gttcggcaaa
     4501 atccgcaaac ggcagcagct ccgaaccgaa acggcgcata tgttccgtgt cctgctggca
     4561 gtctatcagt tccacaccca aatccgccaa aaacggcacg gcgcaggcaa acgcgatttt
     4621 cgacgcatcc ggttgtaatg cgaacatcga ttcgccgtaa aacaccctgc cgatttgcac
     4681 gccgtaaaag ccgcccgcca acctcgtttc accgctttca tcgggataat ggcactcgaa
     4741 cgaatgcgcg tgtcccattt cgtgcagctt caaatatgcc gtctgaaact cgggcgcaat
     4801 ccaagttccg tcctgattcg ggcgcgccgc tgccgcacaa tgcgcgacca cttccgcaaa
     4861 acagccgttg accgcaaccc gatagctgcc gttgcgcagc gttttcgcca gcgagcgcgg
     4921 aacgtgcaga cgttcgggaa caatcaccgc acgcgggccg accgcatacc aaaaaaacca
     4981 accgtcccga gaaaaccacg gaaacacgcc gttccgatac gcctcgagca gccgccccgc
     5041 atccaaatcg ccgctcacgc cgaccagccc gtcgcaccgg gccaaagcat aggtaggatc
     5101 gggaaaggca taattgtcag gggcaagcag cggaatacgc atggcagttc tccaaaaaca
     5161 cagacggcat ccaaaatcat gaatgccgtc tgaacaacaa accgtccgga tttaacgttt
     5221 gcccttggcc tgacagtcgc tgcacacgcc gtacatataa agcgcgtgat cgacgatgcg
     5281 gtagccgttt tcctcagcga ttttgtcttg cagggcttcg atttcgggat tgtggaattc
     5341 cgttacctcg ccgcacttca cgcagacgat gtggtcatgg tggtcgcctt tgtccaactc
     5401 ataaaccgcc ttgcccgttt caaaatgatg gcgttgcaga atacccgcct gctcaaactg
     5461 ggtcagcaca cggtaaatcg tcgccacacc gatttccaca ccctcttcca acaaaatgcg
     5521 gtacacatct tccgcactca aatgctcttc cgaatgcgtc tcgaacaaat ccaaaatctt
     5581 caaacgcggg ccggtaacct tcagaccgct gtctttcagt tgtgcaatat tgttgaattt
     5641 ttccataata ttcaataccc ctgtaaaaca atagacgtta taatacgcaa tttcggcctg
     5701 tttgcccact atcgcaccat agcagtttgc aatagtaaaa ccgcaccggc ggccgatgcg
     5761 cggacagggc ggataccgct taatcgtatg attatcgttc gcttttataa aatattcaag
     5821 cagtgttaca ctacccatcc cgatttgcac agaaaggcat ttccgtgaac aaaaccctca
     5881 tcctcgccct ttccgccctc cttggccttg ccgcgtgcag tgccgaacgc gcctcgctgt
     5941 tcccctcata caagctcaaa gtcatacagg gcaacgaaat cgacccccgc gccgccgccg
     6001 cactccgcct cggtatgacc aaagaccaag tcctgctcct gctcggcagc cccctgttgc
     6061 gcgacgcgtt ccacaccgac cgctgggact ataccttcaa cacctcccgc aacggcatca
     6121 tcaaagaacg cagcaatctg accgtctatt ttgaaaacgg cgtactcgtc cgcaccgaag
     6181 gcgacgccct gcaaaacgct gccgaagccc tcaaagaccg ccaaaacaca gacaaaccat
     6241 aaggaacaca catgacaccg ctcaaaatcg ccatcgccgg cgcaaacggc cgtatgggac
     6301 gcgtattggt tgaagccgtc aacaaccatc ccgacaccgt cctttccggc gcgctcgaac
     6361 actcaggctc agaagccctc ggactggacg caggctacgc cgtcggactc aaaaccggca
     6421 tcgccatttc agacgacgtt gacgccgttc tcgcacaaag cgacgtactc atcgacttca
     6481 cccgccccga gcccaccctc aaacacctgc aaaaatgcgt tgaaaaacaa gtcaacatca
     6541 tcatcggcac aacaggcttc gacgatgcgg gcaaagccgc tatccacact gccgccgaac
     6601 aaacaggcat cgttttcgcc gccaacttca gcgtcggcgt caacctcacc ttccacatcc
     6661 tcgacaccgt cgcacgcgta ttaaacgaag gctacgacat cgaaatcatt gaaggccacc
     6721 accgccacaa agtcgatgcc cccagcggca ccgcgttacg catgggcgaa gtcatcgccg
     6781 gcgcgctcgg cagagacctc aaacaatgcg ccgtttacgg ccgcgaaggc cacaccggtc
     6841 cgcgcgatcc gtcgaccatc ggctttgcca ccgtccgcgc aggcgacatc gtcggcgacc
     6901 acaccgccct cttcgccacc gacggcgagc gcgtggaaat cacccacaaa gccggcagcc
     6961 gcatgacctt tgccgccggc gccatccgcg ccgcagtttg ggtaaacggc aaaacgggtt
     7021 tgtacgatat gcaggacgta ctcgggctga acaaccgtta acccccatac aaaatgccgt
     7081 ctgaaaagat attgttcaga cggcattttg ccaacaggct ccgtatcggc atatcaataa
     7141 agttgcttgc gcggcatgac aagctacggc tcgtttaatt gaaaccctcc tgatctaaaa
     7201 attctaactc tatttcccgg caaactatat ctatcctaaa agcagtcgga aaatcatacc
     7261 taccgcctat caattccagc gcacctccat aatccaagat ttcatcatcc tgctttaatt
     7321 cgccaagttc caatgtatta tcgatattta tatcttggct ctttgtagcg tatccataga
     7381 caaatttcag atagctgaac tgcttgaatt taatttttac ccagtctgta taatttggtg
     7441 taggaatctc ttgaggaaag tccggataat ccacattctg gacgatgata tggaattcat
     7501 cgtttttcaa agtatagttt aatagagtgc cctcatatat atacagaaat tgataatctt
     7561 tttttggaat ggtataagtt gtcatattat tttacctttt caaaaacaat gagtttaaga
     7621 ttttttgagt ccaagttggt tctactactc ttatgaaatt tcagttgttt aaactcttcc
     7681 tttaacggcc cattatattt taaccttaat cccactcgag ccagttctcc ttcatttgga
     7741 attcctctta aatacttatt gtttgatgtg ccattaatca caatttctcc cccctttttt
     7801 tgtaatacgt gctgcttcaa gaagataatc cattaaccgc tcaacatccc gcctcttcca
     7861 aaaccgcgcg cgcggccaaa tccgccgtat cttttttcaa cagcaggtgc aacgccctga
     7921 aatcctgttg cagcgcggca accttatcgg ggtgttcgta ccagtccgcc aacgccgccg
     7981 ccagtttttc cggtacggca tcatgttgaa gcagctccgg cacagcctcc ttacccaaca
     8041 ggatattcgg caggccgaca tgcggcactt tgattttgcg tttcacataa gcataagtca
     8101 gcggcgaaat cttgtagctg atgaccatcg gacgcttgca cagagccact tccaaagttg
     8161 ccgtaccgct cgttaccagc accgcatccg ccgccctgca cactgtttca gactgtctgt
     8221 cgattaccgt cagcggcaat ccggcaaact ccggccgctg caaaacttcc gccaaacgcc
     8281 gcttcgtcgc cgccgttgcg gcaggcagca ggaagcgtgc ggcaggatag cgttccaaca
     8341 acaataatgc cgtctgaaaa aacaccggcg ccatatagtc gatttcgctg acgcggctgc
     8401 cgggcagcag ggcgaatacg gggatgccgg catccacgcc caaagttttc cgcgccgttt
     8461 cacggtcgtc ttccaagggc ataagctgcg ccatcggatg accgacaaac tccgcacgtc
     8521 cgcccgcatc gagataaagc tgcggctcca tcgggaacag gcacagcacg cggttgacct
     8581 gatgcacgat tttgcccacg cgttcccgcc gccacgccca caccgacgga ctgacataat
     8641 gcagggtcgg aatgcccgcc cgtttgagcc gctccgccac acccagatta aaatcgggcg
     8701 catcgatgcc gacaaacaca tcaggtttca acgacagcaa atcccgtacc agccccctgc
     8761 gtatccgtaa aatttccggc aggcgtttta ccacctcggc aaagccgcgc accgccagtc
     8821 gctcctgatc ataaaggctc tcgaaacctt ccgccttcat cagttcgccg ccgataccga
     8881 caaaacgcgc ctgcgggcaa cgctttcgga tggcgcgtat caggtgcgcc cccaacaggt
     8941 cgcccgacgc ttcgccgaca ctgacagcaa tcaaagggct ttttttatca accatattcg
     9001 ttcttcttca catactttca cgtcccgatg ccgtctgaag gcttcagacg gcatcggggt
     9061 tcacattttc gtcccgtttt ccaacatcac cgcaaactgt gccaaatcca gcggcagctc
     9121 cgccttcaac accagcggct cgcccgtgag cggatggttc aagtgcagct cggacgcgtg
     9181 caaaaacatc cgcttcaaac ccaacttctg caaacgacgg ttcgcctgat aatcgccgta
     9241 gcgttcgtcg cccgcaatcg gacagccttg agattgcagg tggacgcgga tttggtgcgt
     9301 gcgccccgtt ttcaacgtcg cccgcaccaa agtcaggtgc gacagcccga caccgtgcaa
     9361 aatgccgtct gaaaaacggc ttaacacacg gaacaccgta tgcgccgact gcccgtccgc
     9421 actgacgcgc accatctttt cgccttgtgc gccggtatat ttgaacaggg gcagtttgac
     9481 atggaaattg tcgtccggca gtttgcccac ccccagcgca aggtagattt ttttggggtg
     9541 gtcgttacgg atggcttcgt gaagtttgac gagcgcgctg cgtttcttcg ccaccatcaa
     9601 caagccgctc gtatccttgt ccaaacgatg aaccaactcc aaatacttcg cctccggacg
     9661 ggcgcggcgc aactgttcga taacgccgaa actcacgccg ctgccgccgt ggacggcaac
     9721 gccggacggt ttgtcgatga ccaaaagcgc atcgtcttcg taaacaacgt caaacgcacg
     9781 cgccggtacg gcggcacgcc tttcagacgg catttccttc tccgccacgc gcacaggcgg
     9841 aatccgcacc gtatccccct ccgcaatacg gctgtcgggt ttgcagcgtt tcttgttcaa
     9901 ccgcacctcg ccggcgcgga taatgcggtg gatatggctc ttgggaacac ccttgaggat
     9961 ttttatcaga tagttatcaa ggcgttgacc cgcctcatgt tcggcaaccc ctatcaagct
    10021 gaccgaatct ttgcttattt cgtgcgtttt catctataat ccgaacatcc gtttcagcaa
    10081 aaagcgcgcc cgccgcgccc ccctgaaacg gtttacttta aaacggaatt ttatattaaa
    10141 aacgcactcc gcaaagcatt ttccctgcgc ctgttcccag ccggcaggcg cagaacgtaa
    10201 tcaagtttga attgattttg ccgtttcggc gcggaagtaa gacggcagcc gggccccaag
    10261 tcccaaacgc aaaccgccca aaacacaggc atcacgataa caagaagaag ccggccccca
    10321 tttttccgac cgcagcgttc ggaacgcggc agacaaaccg ccgcatcgat ctttatagcc
    10381 ttctgtatcc gacccttccg cacacggttc tccggaacgt gcatccctgc ggattttcaa
    10441 ctcaaaaact gattaccggt tttatccgaa aacatcggaa aacccagcct tacggaatgc
    10501 cgaaccggac cggcgcgccc gaatgccgcc cgtccacgag gtgatcatga aaagaatgtt
    10561 attcaacgca acgcaggccg aagagctgcg cgttgccatc gtcgacggac aaaacctgct
    10621 ggatttggac atcgaaacgc tgggcaaaga acagcgcaaa ggcaatatct acaaaggcat
    10681 cattacccgc atcgagccgt cgctggaagc gtgtttcgtg gattacggaa ccgaccgcca
    10741 cggcttcttg ccgtttaaag aagtctcccg ctcatacttc caagactacg aaggcggacg
    10801 cgcgcgcatc caagacgtgc tcaaagaagg catggaagtc atcgtccaag tcgaaaaaga
    10861 cgagcgcggc aacaaaggcg cggcgctgac caccttcatc agcctcgccg gacgctatct
    10921 ggtattgatg ccgaacaacc cgcgcggcgg cggcgtatcc cgccgtatcg aaggcgaaga
    10981 acgtcaagaa ctcaaagccg ctatggcgca actcgacatt ccgaacggca tgagcatcat
    11041 cgcccgtacc gccggcatcg gccgcagcgc ggaagagttg gaatgggatt tgaactatct
    11101 caaacaactc tggcaggcga ttgaagaagc aggaaaagcg catcacgacc cttacctgct
    11161 ctttatggaa agctcgctgc tcatccgcgc catccgcgac tatttccgcc ccgacatcgg
    11221 cgaaattctg gtggacaatc aagaagttta cgaccaagtt gccgagttca tgagctacgt
    11281 catgccgggc aatataggcc gtctgaaact ctacgaagac cacacgccgc tgttttcccg
    11341 cttccaaatc gaacaccaaa tcgaaagcgc gttttcgcgc agcgtcagcc tgccctccgg
    11401 cggcgcgatc gtcatcgacc ataccgaagc cctcgtctcc atcgacgtga actccgcacg
    11461 cgccactcgc ggcgcagaca ttgaagacac cgcgttcaaa accaatatgg aagccgccga
    11521 agaagtcgcc cgacaaatgc gcctgcgcga cttgggcggc ttggtcgtca tcgacttcat
    11581 cgacatggaa aaccccaaac accagcgcga tgtggaaaac gtcctgcgcg acgcgctcaa
    11641 aaaagaccgc gcccgcgtac agatgggcaa actctcccgt ttcggacttt tagaattgag
    11701 ccgccaacgt ttgaaaccgg ctttgggcga aagcagccac gtcgcctgtc cgcgctgcgc
    11761 cggcaccggc gtgattcggg gcatcgaatc caccgccctg cacgttttgc gcatcattca
    11821 agaagaagca atgaaagaca acaccggaga agtgcgcgca caagtgcccg tcgatgtcgc
    11881 caccttcctg ctgaacgaaa aacgcgccga gctgtttgcg atggaagagc gtttggatgt
    11941 caacgtcgtc ctgattccga acatccacct cgaaaatccg cactacgaaa tcaaccgcat
    12001 ccgcaccgac gacgtagaag aagacggcga accgagctac aaacgcgtcg ccgagcctga
    12061 agaagacgaa tccgccaaac cgttcggcgg cgaaaaagcc aaagccgccc gtcccgaacc
    12121 cgccgtcaaa ggcgtgcgcc acaccagccc cgccccgact gccgcccccg agaaaaaaac
    12181 ctcttggtgg gacagcttca aagcttggct caaacgcatt ttcggcggca gcgaaaccca
    12241 agccgcgccc gctgccgaaa cctccgaaaa acgcagcacg gcaaaccgca gcggcagccg
    12301 cgccaacaac cgccgccaaa acccgcgccg cagcaaacgc gaaggcagca aagtagaagt
    12361 ccgcgaagtg gcaggcaaaa ctgccggaca ggaagcgcgt gccgacaaag ccgaaacgcg
    12421 caacaacggc aaccgccgcc gcaacgaacg cggcgaccgt gccgccggac gcgccgacga
    12481 agcggaaatc caagaccgca acatacagcc tgccgcaacc gttgcagatg ccgcaccgtc
    12541 cgaaaccgaa gtgcaaaccg gaaaacgccg ccgcaacggc agccgcagcg aacgcggcca
    12601 aaccgcgccg gaaaccgcca ccgttgccga aacaaccgtt cagacagcgg aaaacacgcc
    12661 gtccgaaccg cataccgcag aagacaaagg cagcaagccc aaatccgaac gcaaccgccg
    12721 cgaacgcgac agccgagatg ccaaagaacg ccgcgagcgc aacaatcagc gcgaccgccg
    12781 tcaaaacggc aaaaaacaca atattccgtc tgccgccaaa atcgagcagt acctgaatat
    12841 tcacgacacc gccgacaaag tccgttccgc cgccgcgcac gttttcggcg aaaccgacgc
    12901 aaacgcgccg attaccgtca gcattgccga tccggttgca gaaagggatc ttccgacagc
    12961 atctcccgcc gtttcaaacg gcgacgcacc ggtttatgat gcggcggaaa aaatccgccg
    13021 tgccaccgcc gccatcctgc ccgaaggcgc gacaccgaaa gccgaagcac aggaaatgcc
    13081 gtctgaaaac gcaaccttta cggctgcggc ggaacaggca cgggaaaccg cacaaaccgg
    13141 cggactcgtc ctgatcgaaa ccgaccctgc cgcattgaag gcatgggcgg cacaacccga
    13201 agtccaagcc ggacgcggtt tgcgccgttc cgaacagccc aagccgtctg aagccgcaac
    13261 cgtccctgcc gaagaaatga tccaagtcga aacccggcaa ggctgaaccg acggcggcaa
    13321 aaagaggttc tgttccgcag aacctctttt ttacatgggt tcggatacct gcaatgccgt
    13381 ctgaaacttc gccattcccg tgattaccga aacattccgc cattcccatg attcccgcaa
    13441 cattccgtca ttcccatgat tcccgcaaca ttccgtcatt cccatgaaaa cgggaatcta
    13501 gaacctcaaa ctttcggata atctttgaat attgccgtcg cccaaaggcc tggattcccg
    13561 cctgcgcggg aatgacggcg gagggtggac gatgccgtct gaaacttcgc cattcccatg
    13621 attaccgcaa cctttcgtca ttcccgccac ctttcgtcat tcccgtgaaa acgggaatct
    13681 agaacctcaa actttcggat aatctttgaa tattgccgcc gcccaaaggt ctggattccc
    13741 acaacatt
//
LOCUS       DAWKQB010000044        13620 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.044, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000044 DAWKQB010000000
VERSION     DAWKQB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.044"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(294..629)
                     /locus_tag="WJL14_000948"
     CDS             complement(294..629)
                     /locus_tag="WJL14_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ7656036.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(709..2838)
                     /gene="dnaX"
                     /locus_tag="WJL14_000949"
     CDS             complement(709..2838)
                     /gene="dnaX"
                     /locus_tag="WJL14_000949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ7656037.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1610..1649)
                     /gene="dnaX"
                     /locus_tag="WJL14_000949"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2933..4909)
                     /gene="mutL"
                     /locus_tag="WJL14_000950"
     CDS             complement(2933..4909)
                     /gene="mutL"
                     /locus_tag="WJL14_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ7656038.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMIELLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRANLTE
                     SVGNAGEVLHDITGVVSTPMPSENDSENLFDSASDYPTGNKPDTRNAFGSSGKTAPMP
                     YQAARAPQQHSLSLRESRAAMNTYAELYKKTDDIDLELNRLEQARFGNMPSETPIPKT
                     DTPLSDGLPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     RENGKLQSQRLLIPSTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRTVPAML
                     GKADVVSLAKDVLGELAQVGSSQTIEEHENRILATMSCHGSVRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5052..5237
                     /locus_tag="WJL14_000951"
     CDS             5052..5237
                     /locus_tag="WJL14_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ7656039.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5248..5916
                     /locus_tag="WJL14_000952"
     CDS             5248..5916
                     /locus_tag="WJL14_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ7656040.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPKHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            5916..6632
                     /locus_tag="WJL14_000953"
     CDS             5916..6632
                     /locus_tag="WJL14_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ7656041.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQTYPGSPAAKRAAAAVRKR"
     gene            6680..7165
                     /gene="purE"
                     /locus_tag="WJL14_000954"
     CDS             6680..7165
                     /gene="purE"
                     /locus_tag="WJL14_000954"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ7656042.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7348..8802)
                     /locus_tag="WJL14_000955"
     CDS             complement(7348..8802)
                     /locus_tag="WJL14_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ7656043.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8799..9500)
                     /locus_tag="WJL14_000956"
     CDS             complement(8799..9500)
                     /locus_tag="WJL14_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ7656044.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALAGSNIE
                     PIVFEREIDTWKTEFFTNINAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(9497..10276)
                     /locus_tag="WJL14_000957"
     CDS             complement(9497..10276)
                     /locus_tag="WJL14_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ7656045.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAIALIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            10560..11966
                     /locus_tag="WJL14_000958"
     CDS             10560..11966
                     /locus_tag="WJL14_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7656046.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            12078..13604
                     /locus_tag="WJL14_000959"
     CDS             12078..13604
                     /locus_tag="WJL14_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ7656047.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     MYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVFRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2744 a         3665 c         3839 g         3372 t
ORIGIN      
        1 ggctggattc ccgttttcat gggaatgacg aaatttcaga cggcatttaa gcggtacgga
       61 tgtgtaaata atggtagggt gggcttcagc ctgccgattc ccgctattct tgccgttttt
      121 gcgttcttat cattctcact gtttttaccg ttcacgccga atcttgcaaa ctgatgccgt
      181 cattcccgcg aaggcgggaa tccagacccc tcggcacgga aatttatcga gtaaaacggt
      241 ttctcagatt ctacgttcta gattcccgcc tgcgtgggaa tgacggtcgg ggatcagcgg
      301 aagaagtcgc ccactccggg gggtagacct tgcgtgaatg cgcccattgt tttgtttgcg
      361 gtttcttcgg ctttgccccg ggcggatttg agggcggcga ggatgaggtc ttcgagcatt
      421 tctttgtcgt cggcggcttc ttgaatcaaa tcggggctga tgtcgatttt gcgtacttcg
      481 tgcgcgcagg tcattgtgat tttgaccagg ccgttgcctg cttcgccttc gatttcggtt
      541 tcggcaagtt tggcttgcgc ttttttcata ttttcctgca tttgctgcgc ctgtttcatc
      601 aggccgccta atccggcttt tccgaacata ctgaatactc ctgtctgttt tgattgaatg
      661 agggaaacgg caatgccgtc cgaagggttc gggcggcatt atatctgttt acggccggtt
      721 tgctgccaat tccagtgatt cgggctgcca ttgcgcgccg aatgcttgga ggattttttg
      781 tgcggcgggg tcggcttcga gcaatgcttg tgctttttgc ctgtcttcgg cttggacgcg
      841 cttgtcctgc attgcggggg tttctctgcc ggattcgtcg cgccagtctt gggtttgcag
      901 ggtgagttgc agcccgtagg cttgggcaag ggtgtcgcgg attttgtcga ggcgtttttt
      961 gtcggcggtg gcgcgtgctt cggcggtcat tgccaaaacc atcaggccgg tgtcgggatg
     1021 gtattccgtc cacgcggagt gttgtgccgg catttgcgcc gcgccgagtt tgcgggcgaa
     1081 atgccggacg atggctgccc agttttcggt ggaaaattcg ggcggcgggg cggacggcgt
     1141 gttgtttccg ccgatgccgc ctgcttctgc ttcttcgtcc gcgcctccgc ctgcggtatc
     1201 ggcaggggcg gcgtgttccc aatcgggcgg cgggatttct gcgccgtctt ctacggggca
     1261 gtcattatcc ggaaagccgt aaccgtagaa aggttctgca ggggcttcgt gtgcaaatgt
     1321 ttccgtttca acggcttcat cattcggtgt tgcctgaatg gggttttcag acggcacttc
     1381 ggacaagggg gcatcggtta cgttgtcggc tgccttgttc ttggaaactt ggttttcggg
     1441 cggcgtttcg gcttcggatg ccgtctgaat gctttttgcc gacgtttgcg ccgtgccggc
     1501 tgcggcttgg gcttcgtctg gcgcgtcttc ccaaggcgga acatcgttgt tttcttgatt
     1561 ggaaacgggt ccggcagttt tgccttcaga cggcattgcc gctgcggatg ccgtctgaac
     1621 gggtgtttgg gcggtttctg cttcagggcg cggttggggc ttttttacgg cggtttcctt
     1681 ttcggcggtt tgtgccgatg gggattttag ttcggtattt tcaatcacgg cgtttgtatc
     1741 acacgaagct gccgccaagg gcgcaaacgc cagcatacgc agcagggtca tcataaagcc
     1801 ggcgtattcg tcgggggcga ggctcaggtc gcgtttgccg tggacggcga tttggtagta
     1861 aagctggatt tgttcgccgc ttatggtttg ggcgaggcgg tgcaaaatat cggaatcggg
     1921 gtcgtcgtgc gccaaggcgg agggcaccgc ctgtatcagg gcgaggtgtt gcagcagtat
     1981 ggcaagttcg cccaaggcgt tgtcaaagcc gacggcacac gccgccattt cctgcgcttt
     2041 ggcggtcagg gctgcgccgt cttggttgat gatgcctgtc aagagttcgt aaaggtattg
     2101 tttgtcaacc gcaccgatca tttggcggac gtcttgttcg gcaacgctgc ccgaacccat
     2161 ggcgatggct tggtcgagca ggctcaaggc atcgcgcatc gatccggcgg cggcgcgtcc
     2221 caaaagttgc agggcagggg gttcgtaggc gattttttcg ctgtcgagga cgtgggcgag
     2281 gtggtcggca acctgctgcg aagtcatatt gtgcaagaca aattgcaggc agcggctcaa
     2341 gacggtaacg gggactttat gcggatcggt cgtggcaagg atgaatttga cgtgttcggg
     2401 cggctcttcc agcgttttga gcatagcgtt gaacgcgctt ttggaaagca tatgcacttc
     2461 gtcgatgata tagactttgt atttgccggc agtcggtgca tattgggcgt tttccaatac
     2521 ttcgcggatg ttgtcgatgc ctgtgttgga ggcggcgtcg atttcgagca ggtcgacata
     2581 gcgtccggca tcgatttggg tgcagctttc gcacacgccg caaggttcgc cgtgttgcgc
     2641 gttttcgcag ttgaggcttt tggcaaggat gcgggcgatg gtggttttgc ctacgccgcg
     2701 cgtaccggtc agcaggtagg cgtggtgcag cctgccttcg tccaaggcgt tttgcaaggc
     2761 tttgacgacg tgttcctgac cgactaagtc ggaaaaggtt ttgggccgcc attttcgggc
     2821 gagaacttga taggccatgt ttttctcttg gtttcggtcg tgatgtttct gtcggtgcgt
     2881 cggaatgccg tctgaacggc ggcttcgggc ggcgtattct agcactttcg gtttattgtc
     2941 cgcgcaagaa cagtgcgtcc aattctttca aagtcagttt gacccaagtc ggcctgccgt
     3001 ggttgcattg gttgctgcgc ggcgtgtttt ccatatcgcg cagcagcgcg ttcatttccg
     3061 gcagggtaag ccggcggccg gcgcggacgg agccgtggca ggacatagtg gcgaggatgc
     3121 ggttttcgtg ttcttcgatg gtttggctgc tgccgacttg ggcgagttcg cccaatacgt
     3181 ctttggcgag tgagacgacg tcggctttgc cgagcatggc gggaactgtg cggacggcaa
     3241 gggtattgcc gcccatatcg gacagttcca gcccgaagcc tgccagtgct tcggcgtggt
     3301 cggcaagggc ggcgcattct tcgtgggacg cggcaaaggt tgagggaata agcaggcgtt
     3361 ggctttgcag tttgccgttt tcccgacgtt ggcgtttcat tttttcgtag ttgacgcgtt
     3421 cggcggcggc gtgcatatcg atgagcaaca ggctgtcttc ggcttgggca aggatgtaga
     3481 tgcctagcaa ttgggcaatg gcgaaaccga gcggcggcag ttcggattgg gagggaagac
     3541 cgtctgaaag cggcgtatct gtttttggga taggcgtttc agacggcata ttgccgaaac
     3601 gcgcctgctc taatcggttc aactcaaggt cgatgtcgtc ggtttttttg taaagctcgg
     3661 cgtaagtatt cattgccgcg cggctttcgc gcagggacag gctgtgttgt tgcggcgcac
     3721 gcgcggcttg gtaaggcatg ggcgcggttt tgcctgatga accaaaggca ttgcgtgtat
     3781 cgggtttgtt gcctgtcggg tagtcggatg cgctatcaaa cagattttcg ctgtcgtttt
     3841 cagacggcat tggggtggag acaacgccgg taatgtcatg caacacttcg cctgcgttgc
     3901 cgacgctttc ggtcaggttg gcgcgtgtgt cggcaagggc tttgttgagc gtgtggaaca
     3961 caagttggtg cacctgctga ctgtcgcgga agcggatttc ggttttggtc gggtggacgt
     4021 tgacatccac ggcttcgggc ggcaggtcga ggaagaggac gaaagcgggg gtgagggcgt
     4081 tgtgcaaaac gtcgcggtat gcctgcttga cggcgtggag catcactttg tcgcggacaa
     4141 aacgatggtt gacgaagcag tattgtttgt cggttttgcc tttggcaaaa gtcggcttgg
     4201 caatcgcgcc atagagccgc aatgcgccgc tgccgctgtc gatttccaat gatgccgtct
     4261 gaaaatcatc gccgacaatg gcggcaatac gttcatgcag gctttgggcc gggagtttga
     4321 acacttgttt gccgtcgcgt ttgagcgaga aggcaatgtg cggatgcgcc agcgcgagga
     4381 gttcgatcat ggtggcgcag tgggcgtatt ctgtattttc ggatttgagg aacttgcgcc
     4441 gcgcgggggt gttgaagaag agttcggcag cttcgatggt ggtgccgacg gggtgggcgg
     4501 cggcggtggg gctgctgagt ttgccgtctt cggctttgac ttgggttgcg tgcgaactgt
     4561 cttcttgacg gctggtcagg gtcaggcggc tgacggaggc gatgcttgcc aagccttcgc
     4621 cgcgaaagcc catgctggcg acgtgttcca agtcgttcag ggttttgatt ttgctggtgg
     4681 cgtggcggtg gagcgcgagt tcgatgtcgt ccggatggat gccgctgccg ttgtcgctga
     4741 cgcgaatcag gcggatgccg ccgccgtcca gctcgacatc aatcgccgtt gcgcctgcat
     4801 cgatactgtt ttcaacgatt tctttcaagg cgttggcagg gcgttcgact acttcgccgg
     4861 cggcgatttg gttgacgaga tggtcgggca gggcggcaat gcgtggcata aggtaggctt
     4921 ccgttgcaaa aacgctttat tctataataa aaccccttac ttttctgcct gtattcttga
     4981 taaaagccgt ttatcccgtt tggaaaatac cagtataatc acgccgtatc ccgtaaaatt
     5041 ggagcgcaaa gatgtatcac taccaatccg aagccacaca attcctcaac cgtctgattg
     5101 aagaaaaacc tgagttggaa cagcagcgtt tggaaaaccg ggggcttttg tgggatgtcg
     5161 aactcaatcc cgaagaacag gaaaacttcg aggcggcaaa agtagcgaaa aaaccttata
     5221 cctattacca agactgaacc cgccgaaatg acgacccatc tgtccaatgt cgcccccgac
     5281 ctgcaaaact atttgaacgc catcggcgaa cccgaacatc ccgttttgac gcggctgcgt
     5341 gagaagaccg ggcatcaccg tatgggcaaa atggcgattg cgcgcgaaca ggcggcggtt
     5401 ttggtttggc tggcaaagct gattcgcgcg gaaaaatatc tggaagtcgg cgtatttacc
     5461 ggatacagca gcaccgcgct tgcattggcg ttgccaaaac acgggcggat taccgcctgc
     5521 gacatcaatg taacctttac cgatacggcg cgtcaggttt ggaacgaggc aggcgtggca
     5581 cataaaatca gcctgcacct gcaacccgca ttgctgacat tggatgattt gattgcacag
     5641 ggtgaagccg gaagctacga tttggcactg atagacgcag acaaaccgcc cacgccgcaa
     5701 tatttcgagc gttgcctcaa gcttgtccgg caaggcggca tcattgccat cgacaatatt
     5761 ttgttgaacg gaagggtgat gcgcgaagcg gcttccgatg cgccgcccag cgtcggcatc
     5821 ctcaaagatt ttaatcaaaa cctgccgaac gatacgcgga ttgtccccat caccctgccc
     5881 gtcggcgacg gtttgaccct gcttctgaaa aaataatgaa gaccaaatta ccgcttttta
     5941 tcatttggct gtccgtatcc gccgcctgtt cttcccctgt ttcccgcaat attcaggata
     6001 tgcggctcga accgcaggca gaggcaggta gttcggacgc tattccctat cccgttccca
     6061 ctctgcaaga ccgtttggat tatctggaag gcacactcgt ccgcctgtcg aacgaagtgg
     6121 aaaccttaaa cggcaaagtc aaagcactgg agcatgcgaa aacacaccct tccagcaggg
     6181 catacgtcca aaaactcgac gaccgcaagt tgaaagagca ttacctcaat accgaaggcg
     6241 gcagcgcatc cgcacatacc gtcgaaaccg cacaaaacct ctacaatcag gcactcaaac
     6301 actataaaag cggcaggttt tctgccgctg cctccctgtt gaaaggcgcg gacggaggcg
     6361 acggcggcag catcgcgcaa cgcagtatgt acctgttgct gcaaagcagg gcgcgtatgg
     6421 gcaactgcga atccgtcatc gaaatcggag ggcgttacgc caaccgtttc aaagacagcc
     6481 caaccgcgcc tgaagccatg ttcaaaatcg gcgaatgcca atacaggctt cagcaaaaag
     6541 acattgcaag ggcgacttgg cgcagcctga tacagaccta tcccggcagc ccggcggcaa
     6601 aacgcgccgc cgcagccgtg cgcaaacgat agtttttcca tttttccgct ttttacccca
     6661 tccgcaccaa ggaggattta tgatacaaat cggcatcatc atgggcagca acagcgattg
     6721 gcccgttatg cggcaggcag cgcagtttct tgaagagttc ggcgtagaat atgaggcgcg
     6781 cgttgtttcc gcacaccgca ccccggattt gatgttccaa tacgccgaaa ccgcacgggc
     6841 gcgcggcatc aaagccatca tcgcgggtgc gggcggcgcg gcgcatttac ccggtatggt
     6901 tgccgccaag accaccgtcc ccgttttggg cgtacccgtc cccagcaaat acctgcgcgg
     6961 cgaagattcg cttttatcga ttgtacaaat gcccaaaggc gtacccgtcg ccacattcgc
     7021 catcggcgag gcaggcgcgg caaatgccgc attgttcgcc gtttccatgc tcgccaacga
     7081 aacccccgaa ctggcgcaaa aactggcaga cttccgagcc aaacaggaac aggctgtttt
     7141 gagtatggaa ttggaacaga tttaaaaccc gccgtccgcg aatatatagt ggcttaaatt
     7201 taaaccagta cggcgttccc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg
     7261 ccttgtcctg atttttgtta atccactata caaatgccgt ctgaagccct gtttcgggtt
     7321 tcagacggca tttttgcgaa caacttttta tgcctgttct ttttggcgca ttttttctgc
     7381 cattagttcg tgcagggttt tcttcgccgg tttcatcggc acgcggtttt gcgtccaacc
     7441 caactgtttg cgcggggtca ggttgcggaa cttggtggct gcccaaccga aggcgcggta
     7501 ggttttgctg ccgctgaaaa taccgttgaa tgtgcgccac gccatttgtt cgccgaaggt
     7561 atgcgatgcg ccttgccctc ggatggggtg cggcacggtt tcggtcggcg aacgttgcgc
     7621 ttcaacgcgc aaacgctgca tttgttcggt aatcgggatg cgtaccggac agacttccac
     7681 acacgcaccg cacatggtac aggcggtcgg cagatcgcgg gtggcatcaa gacccaacag
     7741 gtgaggggaa ataatctcgc caatcgggcc gggataggtt gtgccgtatg ccgcgccgcc
     7801 gatgcgggta taaaccgggc aatggttcat acacgcgccg caacggatac attgcagggt
     7861 gcggcgcatt tggtcttcgg cataagcctg gctgcggccg ttgtcgagca gaaccaagtg
     7921 catttcttgc ggaccgtcta attcctcact gcggcgcgga ccggtaatca tgttgaaata
     7981 agtggtaatg ttctgaccga tggcagaacg cggcagcaga ctgtacaagg gtggaatatc
     8041 cgacagcttg gcaactactt tttcaatgcc ggtaatggcg atatgcacgg gcggaacggt
     8101 ggtactcaag cgaccgttgc cttcgttttc caccagacac agcgtacctg tttcagcaac
     8161 ggcgaagttc acgccactca aaccgacatc ggcagtgctg taaatatcgc gcagtgcttt
     8221 acgggcgaag ccggtcagtt ggtctacgtc gtccgttaac ggtgtgccga ggttttggtg
     8281 gaacagttcg ctaacctgtt ctttggtttt atggattgcg ggcatcacga tatgggtcgg
     8341 tttttcacct gccatttgga cgatgaactc gcccaagtcg ctttccaccg ctttaatgcc
     8401 ttttgcttca agataatggt tcagctcgat ttcctcgctg accatcgatt tgcctttgac
     8461 catcagcttg ccgtttttgg ctgtgatgat gtcgtggatg atttcgcagg cttcggccgg
     8521 tgtttccgcc cagtgtactt ttacgcccaa cttggtcagg ttttcttcca gctgctccag
     8581 cagggcaggc aatttagaca atgagcgctg acggacgtgt tcgcacaaat cgcgcaagct
     8641 ttgcagctct tcttcgtcgg tcaaaacagc tttgcgtttg gtcatcagca tatccatcgc
     8701 ggtacgcagg cttttacgca aaggcttgtc ttgaagggaa attgcggcgt tttgcttgaa
     8761 agtttccggc ttcatgtgaa atttgatggt ttgcgtagtc atgcgttttc ctccaaatcg
     8821 gtaggggaaa tgtggtcggg caggatggcg aggataacca aatcacgcgg accgtgcgcg
     8881 ccgtaagcaa gcgtcagttg gatgtctgcg gttttggacg gaccggaaat caggaataca
     8941 ttggtcggca taccgttttc caccagtttt tcgccttcga cggcattatg aaactcgttg
     9001 tacatcttgg acgtatcgaa caggcagaaa tgcacgggcg gaacgagact caaagtacgc
     9061 ggttcttcgg ggctggaaaa cagcatcagc gtgccggtgc gggcgatgcc gcattgcgcg
     9121 ccgctgaagc ccgcattgat gttcgtgaaa aactcggttt tccaagtatc gatttcgcgc
     9181 tcgaagacaa tcggttcgat attgctgccc gccaatgcgg cacgggcaat ttgtccgtgt
     9241 tcggtcgcca agggcagcag gatgtttttc aaacccttgc cttctgccgc ttcgcggaaa
     9301 acctgcatcc aattgctttt cgtcacccaa taaatttcgg ttttgaccgc gcgcattgcg
     9361 gcagcccaat gtttcagacg ctcggcttcg ctgtcccaag aaacgccctt ttcgcggtaa
     9421 taatcgaaaa ccgcaggctc ttccatcggc aaggcatcgg cttttttcag ttttgccaaa
     9481 atattttcgc gcgcgctcat gctttgcctc cggtgcgttc caacaagaag gatgcgatat
     9541 gtttcggacg cggcatatcc ggctcgtcct tggcgatttt gccgccgatg ttcatcatac
     9601 agccgcagtc cgcgctgatg atttcggttg cgccggtttc tttcagcgcg gcgactttgt
     9661 ctgttaccat tgcgccggaa atatcggctt gtttgacgga gaatgtgccg ccgaagccgc
     9721 aacattcgct ttcgtggtcg tggacgatgc gttcgacgtt ttccataccg tcaatcagtt
     9781 gccagcctga aagatggaca ttcatttcgc ggcgggcggc gcaggaagtg tgaacggcga
     9841 ctttgagcgg ttcgcccttg tcttcgggtt tgaaaccgat ggcaagcagg aaatgggtaa
     9901 actcgatgat gcggccggcg caatccacag ccctttcctc gtactcgctg cctttaaaca
     9961 gcgtcggcca atggtgtttc atcataccgc cgcacgaacc ggacggtacg acaatcggcc
    10021 agttttcggg gaaaaggtca agttgcgctt tggcgacatc gaaggcttcg gtcggatggc
    10081 cggatgaata ggcaggctgg ccgcagcagc tttgcgccat cgggaaatgg acgcgtatgc
    10141 cctgctgctc gattagggca atggcatcca tgcctgcttc gggcataaaa agatcaagga
    10201 cgcaagtgcc gaaaaaatag acatcggtcg gattgctgtc gtatcggatg atttttgggg
    10261 gaatggttgc gctcatgttt tttgataacg ggaaacccgt ttttttctgt agaaaggtaa
    10321 gcgtttactt taagtaattg actgttgcgg gtcaagtcta attttaaaaa ataatccggt
    10381 ttttcttaca aactacccca taacgcttac tgtaccttaa tctgatgttt ttcgataata
    10441 attatcatta caatgcaata ccggttcgtt tgcttgtgaa cattcaagat gccgactctg
    10501 acggcattca gacagcgtct gaaaacaata acggcacaag gatggggtac aatcgcccta
    10561 tggaaaaaaa caccctatcc gcccgcgcac catccccttg gctgccgctt ctgctggcaa
    10621 ttgccatttt tatgcagatg ttggatgcga ccattttaaa taccgcactg cctgaaattg
    10681 ccaccgacct ggacgaatcg cctctagata tgcaactggc agtcgttgcc tatacgctga
    10741 cggttgccct gctgattcct ttgagcggtt atttggcgga caggttcgga acgaaaaaag
    10801 tctttttcgg ttcgattgcc gtttttatgc tcggatcggc attgtgcgcc gcatcgggtt
    10861 cgctgtttga attgacgctt tcccgtgtcg ttcagggcat cggcggttcg atgctggttc
    10921 cgataccgcg tctgaccatc ttgcgtgtgt acgacaagtc caagctgctc aatgccatca
    10981 attatgcggt tatgcccgca ttaatcgggc cggttttagg gcctttggcg ggcggttatt
    11041 tggtcgaata cgcttcgtgg cactggattt tcctgctcaa cctgcccatc ggtctgctgg
    11101 gtttcatatt gggacgcaac atcatgcccg atattaaagg cagtaatatc tctttagact
    11161 tcaaaggtta tctgattttt tctgccgccg cgtgcctctt gttactttcg gcagaaagcc
    11221 tgtcgcacgc gctgcctccg tattttgcac tgttgccgct gtgcggcgga ctgctgtttg
    11281 cacgccgtta tttccgacat atgaaaaccg cgtccaaacc gatttattcc gccgacctgt
    11341 ttctgatacg cactttccgt ctgggactgg cgggcaatct gttcagccgt ctcggcatca
    11401 gctcgattcc ttttctgatg cccctgatgt ttcaagtcgc tttcggcttc ggcgcaagcc
    11461 tgtcgggttg gctggtcgcc cccgtcgccc tgtcttcgct gctggtcaaa ccgctgattg
    11521 caccgctcat gaaacgtttc ggctaccgca cggtactgct ttggaacacc aagctgcttg
    11581 ccgccttcat catgctgctc gccctgcctg atggaaactc gccgctgtgg atttggattt
    11641 tcctctcgct ggcgatcggc gcgtgcaact ccctacagtt ttctgccatg aacacactga
    11701 ccctcgccga tttgcgcccg caacagaccg gcagcggcaa cagcctgatg gcggtcaacc
    11761 aacagcttgc catcagcatg ggcattgttg tcggcgcatt aatccttaaa aactggacat
    11821 ttctgatacc ggcttcttca ggtctgcatt ccgccttccg tatgaccctg ctcagtatcg
    11881 gcggcattac ccttgcctcg tcgttggttt tcaagcgtct gcacgtttca gacggtgcaa
    11941 acctgacacg gaacacaccg tcctaaagcg gtccgcacgc aaaactttta cccgtttcaa
    12001 cgtttggatt atgatgccgc acttccatgc gcgccaaccc caaaacacag gcaatgccgt
    12061 ctgaaaccat atccctgatg aaaacacgca gcctaatttc ccttttatgc ctccttctct
    12121 gttcatgttc ttcatggttg cccccactgg aagaacggac ggaaagccgt catttcaata
    12181 cttccaaacc cgtccgcctg gacaacatcc tgcaaatccg gcacacccct cataccaacg
    12241 ggctatccga tatctatctg ctgaacgacc cccacgaagc ctttgccgcc cgcgccgccc
    12301 ttatcgaatc tgccgaacac agcctcgatt tgcaatacta catctggcgc aacgacattt
    12361 ccggccgact gctgttcaac ctgatgtacc ttgccgcaga acgcggtgtg cgcgtacgcc
    12421 tgctgttgga cgacaacaac acgcgcggat tggacgacct cctgctcgcc ctcgacagcc
    12481 atcccaatat cgaagtgcgc ctgttcaacc ctttcgtctt ccgcaaatgg cgcgcactcg
    12541 gctacctgac tgacttcccc cgcctcaacc gccgcatgca caacaaatcc tttaccgccg
    12601 acaaccgcgc caccatactc ggcggacgca atatcggcga cgaatacttc aaagtcggtg
    12661 aggacaccgt tttcgccgac ctggatatcc tcgctaccgg cagcgtcgtc ggcgaagtat
    12721 cgcacgactt cgaccgctac tgggcaagcc attccgccca caacgccacg cgcatcatcc
    12781 gcagcggcaa catcggcaag ggtcttcaag cactcggata caacgacgaa acgtccagac
    12841 acgcgctcct gcgctaccgc gaaaccgtcg aacagtcgcc cctctaccaa aaaatacagg
    12901 caggacgcat cgactggcag agcgtccaaa cccgcctcat cagcgacgac cctgcaaaag
    12961 gactcgaccg cgaccgccgt aaaccgccga ttgccgggcg gctgcaagac gcgctcaaac
    13021 agcccgaaaa aagcgtctat ctggtttcac cctatttcgt ccccacaaaa tccggcacag
    13081 acgcactggc aaaactggtg caggacggca tagacgttac cgttctgacc aactcgctac
    13141 aggcgaccga cgttgccgcc gtccattccg gctatgtcaa ataccgaaaa ccgctgctca
    13201 aagccggcat caaactctac gagctgcaac ccaaccatgc cgtccctgcc acaaaagaca
    13261 aaggcctgac cggcagctcc gtaaccagcc tgcatgccaa aaccttcatt gtggacggca
    13321 aacgcatctt catcggctca ttcaacctcg acccccgttc cgcacggctc aatactgaaa
    13381 tgggcgttgt tatcgaaagc cccaaaatcg cagaacagat ggagcgcacc cttgccgata
    13441 ccacacccga atacgcctac cgcgttaccc tcgacaggca caaccgcctg caatggcacg
    13501 atcccgccac ccgaaaaacc tacccgaacg aacccgaagc caaactttgg aaacgcatcg
    13561 ccgcaaaaat cctatccctg ctgcccatag aaagtttatt atagaaatat agtggattaa
//
LOCUS       DAWKQB010000045        13385 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.045, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000045 DAWKQB010000000
VERSION     DAWKQB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.045"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..538)
                     /locus_tag="WJL14_000960"
     CDS             complement(110..538)
                     /locus_tag="WJL14_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ7656048.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAESPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WJL14_000961"
     CDS             complement(603..2351)
                     /locus_tag="WJL14_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ7656049.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLLHRLELTGIPSERRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELTVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTVMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WJL14_000962"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WJL14_000962"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ7656050.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WJL14_000963"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WJL14_000963"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ7656051.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WJL14_000964"
     CDS             4754..4924
                     /locus_tag="WJL14_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ7656052.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WJL14_000965"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WJL14_000965"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ7656053.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKALVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6158..6283
                     /locus_tag="WJL14_000966"
     CDS             6158..6283
                     /locus_tag="WJL14_000966"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ7656054.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSDDEVKAAVDYMVNQSGGKF"
     gene            6485..7894
                     /locus_tag="WJL14_000967"
     CDS             6485..7894
                     /locus_tag="WJL14_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ7656055.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQAVVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYYTMDEIHSNMMLGAAGDYLLETAASL
                     TIIMVVSGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7996..11205
                     /gene="recC"
                     /locus_tag="WJL14_000968"
     CDS             7996..11205
                     /gene="recC"
                     /locus_tag="WJL14_000968"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ7656056.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEYCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLALLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVRWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAAFLETLTDIVRIWRQPATVDEWVGRCRGLLKTLFQAEADDQKAVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMTGTNSRQLAQNLIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYAAALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAEIYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAEMRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFVQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(11273..12841)
                     /gene="mtrF"
                     /locus_tag="WJL14_000969"
     CDS             complement(11273..12841)
                     /gene="mtrF"
                     /locus_tag="WJL14_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ7656057.1"
                     /translation="MSQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFGLSVPDPRPVGAKGRADDGLIHIVSLLDADGLIKILTHTVTNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTLDPILAGITQQAAQIIHPDYAVGPEAN
                     WFFMAVSTFVIALIGYFVTEKIVEPQLGLYQSDLSQEEKDFRHSNEITPLEYKGLIWA
                     GAVFVALSALLAWSIVPADGILRHPETGLVAGSPFFKSIVVFIFLLFALPGIVYGQIT
                     RSLRGEREVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFVGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMGYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13172..13384
                     /locus_tag="WJL14_000970"
     CDS             13172..13384
                     /locus_tag="WJL14_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656058.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIAKDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENIHAMIGEIKNAI"
BASE COUNT         3353 a         3241 c         3546 g         3245 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gagattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg tttcggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataatgt tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaccgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat caggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 atgccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgtcagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc aggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc ctttcggacg gaatacccgt caattccaag
     1981 cggtgcagca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg ccggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcgccgg gcgtgaagcc ttacaatgcc ctgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactcgcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccgtt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtgggcggtc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattcccg
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cacctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgtgctctc ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat tcacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggcc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ctggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagccgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gctatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttcgttg cacaaacacg cgatcgaagg
     6181 tttcaatacg atgcccgcaa aaggcggtcg cggcgatttg agcgatgatg aagtgaaagc
     6241 tgcggttgac tatatggtca accagtcagg cggcaaattc taacttgact gagtttccga
     6301 tattatcggt tatgaaaatc cagattcagt attgaatctg gattttttgt tttaacgggc
     6361 atccggtttt aaacgcagac tgcctatttg ttgtaatgaa ttttgacttt agtcataaag
     6421 aattagtctt tattgtttat aatcgcgcat catcacgata gccctatttt caaaaggacg
     6481 attaatggat acacaaatca aaactgaagc cggtcagcag aataaccggc gttatctgac
     6541 cgtttggcgg tggcattttt atgccggtct gctggttatg ccttttctta ccctgcttgc
     6601 cgttacgggt ttgggtatgc tgctgtttgc caatattacc ggtaaggagg gcgagcggat
     6661 tcatgttgtg ccgcaggcag tggtgcagcc cctctctgtt caggcagaag cggcacgcag
     6721 tgccgttaat ccggagactt catctgtcgt ccaatatatt gcaccgcgtg ctgatgatat
     6781 ggttgctgtg ttccgtgtca acaatgaggg taaggcaacc atggttgcgg tcgatcctta
     6841 tacggcaaaa gtggtcaaca ccatgccgcg caatcagggt tggtattaca cgatggatga
     6901 aatccacagc aatatgatgc tcggtgcggc aggcgattat cttttggaaa cagcagcttc
     6961 actgaccatt attatggttg tcagcggctt gtacctttgg tgggtgaaac ggcgcggcat
     7021 caaggcgatg ctgctgccgt caaaaggcag ggcgcgttct tggtggcgga atctgcacgg
     7081 cacgtttgga acttgggtgt cgttgatttt gctgttgttc tgcctgtcgg gtattgcttg
     7141 ggcgggtatt tggggcggca agttcgtaca ggcttggagt cagttccctg ccggtaaatg
     7201 gggtgtcgaa ccgaaccccg tttcagtcgt gccgacccac ggcgaggtat tgaatgacgg
     7261 caaggttaag gaagtgccgt gggttttgga gcttacgcct atgcctgttt cagggacgac
     7321 tgtgggcaaa gacggcatta accctgacga gccgatgaca ttggaaaccg tcgaccgctt
     7381 tgcgcgggaa atcggtttca aagggcgtta tcagttgaat ttgcccaaag gcgaggacgg
     7441 cgtatggact ttgtcgcagg attctatgag ttacgacatg atcagcccgt ttgccgaccg
     7501 cacggtacat atcgaccagt acagcggcaa aatccttgcc gacatccgtt ttgacgatta
     7561 caacccgttc ggcaaattta tggcggcaag cattgcgctg catatgggga ctctgggctg
     7621 gtggagcgtg ttggcgaacg tcttgttctg ccttgccgtc atttttatcg gtatcagcgg
     7681 ctgcgtgatg tggtggaaac gccgtccgac cggagcggtg ggcatcgttc cgccggcgca
     7741 gaaagtcaag ctgccggttt ggtggatgat ggcattgccg ctattggcaa tcgcactgct
     7801 cttcccgacc gcactgcttg ccattgccgt gatttggctg ttggatacgc tgctgttgtc
     7861 gcggattcct gttttgagga gatggtttaa atgaccaaat gccgtctgaa aggttcagac
     7921 ggcattttgt ttgaaggcgg acgggggaaa ggctatataa tcccgaatac ttgaccacag
     7981 cttctgtttg aaatcatgtt ttatctgtat caatccaacc gtcttgaaac gctggcggca
     8041 ttgtttgccc gcattcaaaa agtcaaaccg ctgaaatcgg ctttacagcc cgaacagatt
     8101 attgtgcaga gtcaggggat gcgccgctat ctcaatacct gcctcgcccg cgatttgggc
     8161 gtggcggcga atttgtcgtt cagcctgccc gccggcctga cgtggaagct gatgaaaaaa
     8221 ctgattcccg gtattccgga actcagcccg tttgcacccg aagtcatgcg ctggcggctg
     8281 ctggatttgt tccgcagtga agcattccgg aatacggcag aatttgaaga tgtgaggaat
     8341 gtgctgcaag actatctggg cagcggcgaa tcggcagatt accagcttgc gggacagctt
     8401 gcggacatat tcgaccaata cctcgtgtac cgtcctcagt ggatagacgc ttggcagcag
     8461 ggcaggcggc tcggtttggg cgacgacgaa atctggcagt ccaaactgtg gcgttacctc
     8521 gacgatggca ggcagagcgc gccgcaccgt gtcgcgttgt gggaaaagct gttggaatct
     8581 ttaagcagtg ataagctgcc cgagcgttat ttcgtgttcg gcatttccac gatggcaccg
     8641 atgtatttgc agcttttgca caagctgtcc gaatattgcg atgtgtttgt gttcgcactc
     8701 aatccgagcg ggatgtattg gggcaacgtc atcgaagcgg cgcaaatcct caaaggcggc
     8761 ggcgatcccg atttaactca ggcagggcat ccgctgctcg cctcattggg caagcagggg
     8821 cgcgactttt tcgacttttt gaacgaaatg gaaatagaag gcgaaacgcc ggtatttgaa
     8881 gaaggcggac gcgatacgct tttgcacgcc ctgcaaaccg atattcaaaa cctgaaaatg
     8941 ccgtctgaaa atgtgggaag cgtcaacacg ggcgacggct cgatacgcat cgtctccgca
     9001 cacagcccct tgcgcgaatt gcagatactc aaagacaagc tgttgaaaat tctgcatgaa
     9061 catcccgatt ggcagccgca tgatatcgcc gtattaaccc cgaacatcga atcctatacg
     9121 ccttttatcg aagccgtgtt cggacaggcg cagcccggtg cgcaggcatt gccgtattcc
     9181 gtttcggacg tgaaaataag ccgccgacaa ccgctgtttc atgctttgtc atgcctgttc
     9241 gacttgttgg aaagccgatt tgaagtcgat aaagtgctcg cgcttttaga aaccgcaccc
     9301 gtgttgcgcc gtttcggact gactgaggac gatttgccgc ttttgcacga catggttgcc
     9361 gatttgaacg tccgctgggg tttggacgga gaaatgcgcg gcggcacgga tcagctgttc
     9421 acctggaagc aggcggtaga acgcatgata ttgggctgga tgctgcccga aggcggcaac
     9481 ccgatgtggc aggatgtcag cgcgtggtat gccgacgtga accaaaccgc cctgttcgga
     9541 cgttttgccg ccttcctcga aaccctgacg gatattgtac ggatatggcg gcagcccgca
     9601 acggttgatg aatgggtagg acgttgccga ggtttgctta aaacattgtt ccaagccgaa
     9661 gccgatgacc aaaaggcagt ccaaaacctc gaaaacgaat gggtcaaatg gcaggcggaa
     9721 accgaattgg cgcaattttc cggacagttg ccgcaacaaa ccgtcatccg tcacatccgc
     9781 cgcttcctcg acagcgaaag tgaggcaggc tttttacgcg gcggcatcac tttttgcagt
     9841 atggtgccga tgcgcagcct gccgttcaaa gtcatctgcc tgttgggttt gaacgacgga
     9901 gattttcccc gcaataccaa agccgccgta ttcgacctga ttgccaaaca tcccgccaaa
     9961 ggcgaccgcg cccgccgcga cgacgaccgc tacctcttcc tcgaagccct catcagcgcg
    10021 cgtgaaatcc tgtacctgtc ctacatcggg cgcgacatcc gcaaagacga agaactggct
    10081 ccgtcttctt tgttgggcga actcatcgac acagttgccg ccatgaccgg cacaaacagc
    10141 cgccaacttg cacaaaacct gatagaacag catccgctgc aagccttctc gcgccgatat
    10201 tttcaagaag gcggacgttc agacggcata ttcggcacgc gtaccgacta cgccgccgcg
    10261 ctcggacaaa cgcccgaacc gccgcaaccc tttttcgatc aacccgtaga aaacgccgaa
    10321 cctgttgccg aaatcggaca ggacgaattt atccgcttct ggcgcaaccc tgtcaaagta
    10381 tggcttcagc agcagcttgc gtggagcgaa ccccatatcg gcgaagcctg ggagcctgcc
    10441 gaacccttcg agccgcaaca cgccgatcaa atcgccgaaa tctacatcga agcacggcgc
    10501 gaaggacggg attttgccca aaccgccgcc cgcatcgggg cggaaagcct cctgccgtcg
    10561 ggagagttgg gcagactttg gcagcaggac ttccaaactg ctgccaaaca aatcgacacg
    10621 gcggttttaa acagccccaa actgccaccg ctttcatatg ccataccgtc ggacgggcaa
    10681 atcctgaaag gcagcttggg caatctgtac cgctgcggac aagtgtttta cgcctacggc
    10741 aaacccaacg cgccgcaacg tatcgctttt ctgctggaac acctgatatt ttgcgccgtt
    10801 atgccgtctg aagccgaaat gcggcaaacc tttatcgtcc aatccggaga aaccgaaata
    10861 ttggcggaaa tcgcgcaaga cagggcattg cagctattgt cggaatggat ggcgtttttc
    10921 aatatcgggc aaaaccgccc gctgccgttt tttgccaaaa cctcgcttgc caccgccgaa
    10981 gcgtttgtcc aaaaacaaga ttgggaagcc gccctgaaaa aagcccaaac cgcctaccac
    11041 ggcagcaaag tcagcaaagg gcagaaagac tataccgaag tcgccctcgt gttcggcaac
    11101 gcaagccaaa acccgctcga acagcccctg tttgaaaacc tcgtccgtct gcttgccgac
    11161 acgcttgccg cagcggaaaa aaaggaagag gccggagaag cctgacggga tagaaaggca
    11221 aatgccgtct gaaagcgttt aagcatttca gacggcattt tgtttatcgt gtttaaggtg
    11281 cgggatagaa tgtgggcgcg ccgggaccga cgggcaggcc caaaacaaat acccaaatgc
    11341 agaataaggc aatccacgca atcaagaaga aagcggaata cggcaacatc atagaaatca
    11401 gcgtacccac gcccgcatct tttttgtatt tgatcaccgt cgccataatc agcccgaaat
    11461 aacccatcat cggcgtaata atattggtaa cggaatcacc gatgcggtaa gcggcttgaa
    11521 tgacttcggg cgcgtaaccg gccaacatca gcatagggac gaaaatcggc gcagttaccg
    11581 cccattgcgc ggaagcggag cctatcatca gattgataaa ggcacaaatt aaaataaaac
    11641 cgacaaacaa cacgctgccg cccaggccga cttcttttaa gaacgtcgcc cctttaacgg
    11701 caatatattg cccgatattc gtccaattaa aaaatgcgac aaactgtgcg gcaaaaaaga
    11761 tgatgaccaa ataaagtccc aaagtactca tcgattcggc catcgcatta acgacttccc
    11821 gttcgccgcg caaacttcgg gttatctgtc cataaacaat gcccggcagc gcaaacaaca
    11881 agaaaataaa aacaacaatc gatttgaaaa acggcgaacc tgcaaccaat cctgtttcag
    11941 gatgacgcaa aatgccgtcg gcagggacga tgctccaagc caataaggcg gataaagcaa
    12001 caaacaccgc gcctgcccaa atcaatcctt tatattccaa aggcgtgatt tcattggaat
    12061 gccgaaagtc tttttcttct tgtgacaaat ctgactgata aaggcccaat tgcggttcga
    12121 cgattttttc agtaacaaaa taaccaatca aagcaatcac aaacgtactg accgccataa
    12181 aaaaccaatt ggcttcaggg cctacggcgt agtcgggatg gatgatttgc gccgcctgtt
    12241 gggtgatgcc tgccaagatc ggatcgagcg tgcctaagaa caaattggcc gaataaccgc
    12301 ccgaaacacc ggcgaaagcc gcagccagac cggcgagcgg atggcggccg agcgaatgaa
    12361 agatgacggc ggacaaagga atgaggacga catagcccaa ttcagaagcc gtattggata
    12421 aaatccccgt aaaaacaacc ataaaagtag tgagtttgcg tggagatttt gtgagcaata
    12481 agcgcattaa tgcagaaatc aagcccgatt tttccgcaat ccccacgccc aataaagaaa
    12541 ccaacaccgt tcccaagggc gcgaaaccgg tgaaatttgt aacggtatgc gtcaggattt
    12601 taatcaaacc gtcggcatcg agcaggctga caatgtgaat caaaccgtca tcggcacgtc
    12661 cttttgcccc aacagggcgc gggtcgggca cggatagtcc gaaatacgca ccggcggcag
    12721 aggcaatcag caataacaca atgaaaataa taaaaagcgt aaccgggtgc ggcaacatat
    12781 tgcccagcca ttcgactgtg cgtaaaaatc gcccgccccg tcgcgtatcg gtttgactca
    12841 tctgtttctc cttcaaaaaa tatatcgggt gtattaaatt ttgaatgtaa caggaagtgt
    12901 tttgtaagca aactaaaata attgcatatg tatataaccg caatagatta aaacagaaaa
    12961 tgccctcatc gttattatcg taaaggcggg aatccgaaag ctcgacgtta aagccttatc
    13021 aggaatgact ggaactcaaa aaacggtttc ccgctttcgc ggaaatggcg ggattttcgg
    13081 tttttatttg caaaaatgcc ggagaagaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccaaagatgt
    13261 atcgctgatg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatatcca tgcgatgata ggggaaatta aaaatgcaat
    13381 ttagt
//
LOCUS       DAWKQB010000046        12998 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.046, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000046 DAWKQB010000000
VERSION     DAWKQB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.046"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            48..1301
                     /gene="murA"
                     /locus_tag="WJL14_000971"
     CDS             48..1301
                     /gene="murA"
                     /locus_tag="WJL14_000971"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ7656059.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            1382..2560
                     /locus_tag="WJL14_000972"
     CDS             1382..2560
                     /locus_tag="WJL14_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ7656060.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     KYCLDNGASVIVMTHLGRPTEGEFHSEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(2628..2876)
                     /locus_tag="WJL14_000973"
     CDS             complement(2628..2876)
                     /locus_tag="WJL14_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ7656061.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(2967..3884)
                     /gene="ftsX"
                     /locus_tag="WJL14_000974"
     CDS             complement(2967..3884)
                     /gene="ftsX"
                     /locus_tag="WJL14_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ7656062.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLVRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(3881..4531)
                     /gene="ftsE"
                     /locus_tag="WJL14_000975"
     CDS             complement(3881..4531)
                     /gene="ftsE"
                     /locus_tag="WJL14_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ7656063.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(4726..5208)
                     /locus_tag="WJL14_000976"
     CDS             complement(4726..5208)
                     /locus_tag="WJL14_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7656064.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCQKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            5332..5685
                     /gene="arsC"
                     /locus_tag="WJL14_000977"
     CDS             5332..5685
                     /gene="arsC"
                     /locus_tag="WJL14_000977"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ7656065.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            5682..6338
                     /locus_tag="WJL14_000978"
     CDS             5682..6338
                     /locus_tag="WJL14_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ7656066.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPFLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKHPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            6454..7848
                     /locus_tag="WJL14_000979"
     CDS             6454..7848
                     /locus_tag="WJL14_000979"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ7656067.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTRWADL
                     VKGEISIPNEALDDLIIARSDGSPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            7869..8264
                     /locus_tag="WJL14_000980"
     CDS             7869..8264
                     /locus_tag="WJL14_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656068.1"
                     /translation="MPSDVGIRLQTASKRKLNMKKILYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            8268..8759
                     /locus_tag="WJL14_000981"
     CDS             8268..8759
                     /locus_tag="WJL14_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7656069.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMKSFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(8883..11477)
                     /gene="mutS"
                     /locus_tag="WJL14_000982"
     CDS             complement(8883..11477)
                     /gene="mutS"
                     /locus_tag="WJL14_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ7656070.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTNLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            11921..12925
                     /gene="gap"
                     /locus_tag="WJL14_000983"
     CDS             11921..12925
                     /gene="gap"
                     /locus_tag="WJL14_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ7656071.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
BASE COUNT         2957 a         3189 c         3795 g         3057 t
ORIGIN      
        1 gatttttgtt aattcactat acaagtaccc actaggaaag aacaaacgtg gataaactga
       61 aaatctccgc aaacggcccg ctcaacgggg aaataacggt ctcgggcgcg aaaaacgcgg
      121 cattgccgct gatgtgcgcg ggtttgctga catcgggtac gttgcgcctg aaaaatgtgc
      181 ctatgctggc agatgtgaaa accacgcaaa agctgcttca ggggatgggc gcgcgcgtcc
      241 tgaccgacaa tatcagcgaa tttgaaatca acggcggtac ggtaaacaat acctgcgcgc
      301 cgtatgagct ggtcagaact atgcgcgcct cgattttggt gcttggcccg acgctggcgc
      361 gtttcggcga ggcgcaagtc agcctgccgg gcggctgcgc catcggttcg cgccccgtcg
      421 atcagcattt gaaaggcttg gaagcgatgg gggcggaaat cgtcatcgaa cacggttacg
      481 tcaaagccaa aggcaaactc aaaggtacgc gcgtggcgat ggatgtcgtt accgtcggcg
      541 gcacggaaaa cctgctgatg gcggcgacgc tggcggaagg tacgacggtt ttggaaaact
      601 gcgccatcga gcctgaagtg gtcgatttgg cggaatgcct ggtcaaaatg ggcgcgaaaa
      661 tcagcggcat cggtacgtcc acaatgattg tggaaggtgt ggacgagctg cacggctgcg
      721 aacacagcgt tgtccccgac cggattgaag cggggacgtt cctgtgtgcg gtggcgataa
      781 ccggcggcag ggtggttttg cggaatgctg cgccgaaaac gatggaagtg gtgttggaca
      841 aactggttga ggcaggtgcg gtgattgagg cgggcgacga ttggatcgcc atcgatatgc
      901 ggcagcgtcc gaaggcggtg gacatccgca cggtcgtcca ccccggcttc cccacggata
      961 tgcaggcgca gtttatggca ttgaatgccg tggcggaggg aagctgccgc gtggtggaaa
     1021 cgatttttga aaaccgcttt atgcacgtcc ccgagttgaa ccggatgggg gcgaacatta
     1081 caaccgaggg caatacggca tttgtgcagg gtgtggaacg gctttccggc gcagtcgtca
     1141 aggcgacgga tttgcgtgcg tccgcaagcc tcgttatcgc cggtttggcg gcgcgcggcg
     1201 aaaccgtggt cgaacagatt taccacttgg atcgcggtta tgaaaatatt gaaaaaaaac
     1261 tcggcagcgt cggcgcgaaa atcgagcgcg tatccggctg atcgggttgc gatgccgtct
     1321 gaagccttca gacggcatca attgtttctg tatcgcaaac actgattgaa aaggactgat
     1381 tatggcattt ttaaaactga ccgaacaaaa cgtgcagggc aaaaccgtcc tcattcgcgc
     1441 cgatatgaac gtgccgttca aagacggcaa aatcagcgac gacacccgta tccgcgcctc
     1501 acttgcgtcc atcaaatact gcttggacaa cggcgcgtcc gtcatcgtga tgacccactt
     1561 gggtcgcccg accgaaggcg aattccattc cgaagacgat gtcgcgcccg ttgccgcgca
     1621 cttgggcggt ctgttgggca aagacgtgaa agtattgaac gactggcgtg aaaacaaacc
     1681 ggctttgaac gcgggcgatg tcgtgatgct gcaaaacgtg cgtatcaaca aaggcgagaa
     1741 gaaaaacgat ttggaactgg gcaaagccta cgcttctttg tgcgacgtgt tcgtcaatga
     1801 cgcgttcggc accgcgcacc gcgcccaagc ctcgaccgaa gccgtcgccc aagccgctcc
     1861 cgttgcctgc gccggcgtat tgatggcggg cgaactcgac gctttgggca aagccttgaa
     1921 acagcccgcg cgcccgatgg ttgccatcgt tgccggcagc aaagtgtcca ccaagctgac
     1981 catcctcgaa tcgctggcgg acaaagtcga ccaactcatc gtcggcggcg gtatcgccaa
     2041 caccttcctg ttggcggaag gtaaagccat cggcaaatct ttggcggaac acgatttggt
     2101 ggaagaatcc aaaaaaatta tggcgaaaat ggcggccaaa ggaggttctg tgccgctgcc
     2161 gaccgatgtc gtcgttgcca aagcctttgc cgccgatgcc gaagcggtgg taaaagacat
     2221 tgccgacgtt gccgaagacg agatgatttt ggacatcggc ccgaaatctg ccgccgcgct
     2281 tgccgatttg cttaaagccg ccgacacggt cgtttggaac ggaccggtcg gcgtgttcga
     2341 gtttgaccag ttcgcaggcg gtacgaaagc ccttgccgaa gccattgccc aaagcaaagc
     2401 gttctccatt gctggcggcg gcgacacgct ggcggcgatt gccaaattcg gcgttaccga
     2461 ccaaatcggc tacatctcca ccggaggcgg cgcgttcctt gagttcttgg aaggtaaaga
     2521 gttgcctgcc gtagccgctt tggaaaaacg cggcgagtaa acggtttgat ataaaaaaat
     2581 gccgtctgaa acgttgtgct gtttcagacg gcatttttgt gtggcggtta ttgtgctttg
     2641 gcattccatt ctgccggtgt ggcggctacc gaaatggaaa gtgcgtgcag ttcgttgctt
     2701 tccagttggg ctttaagccc gtctttaatc aggcggtggc gcgcgaggcg tgccttgcct
     2761 tcaaattctg atgaaacgat gacggcgaaa aaatgatgtc cgtcgccttc tacttcgatg
     2821 tgttcgcatt ttgccacgcc ttcaatcatg gcttttactt gttccgaggt cagcatggta
     2881 ggttccttgt tggaaaaatg cgccattata ctggcaaatt gaaatgccgt ctgaaaacgg
     2941 gttcggacgg catttttgac ggtgttttat tttttggctt tgaagccgag caggtgctgg
     3001 gtggtggcaa gccacgcgcc gaatatcccc agtgcgatga cgaagccgaa caccagtgcg
     3061 atttctcccg ggtagaagaa acgccagccg atattaagtc cgtagggttt gaagatggca
     3121 tcgaccaagg ggcgcacggc agagagcagc caaccgcaaa gccccaagct gacggcggcg
     3181 gaaaggatgc tctgccacat ggcttgataa aggaatgggc ggcggataaa cgacgcgggc
     3241 gcgcccaaga gtttggtgat ttcgatttct tcttttcggc tgaggatttg cagtcggatg
     3301 gtgttgtgcg cgacaaggac gaacgccatc cccagcgtca gggaaagaaa ccataagatt
     3361 ttacggatga attcgttgat ttggtacagt gtctgtaccc attccgtatc catgttggcg
     3421 gattcgacca taggcagttt ggtaatgtcc cggtagattg cctgcatttg ggcgggcgtg
     3481 gttgccgggt cgggggtaac gataaagaca tccggcaggg ggttaccgtc aagcatggaa
     3541 atcagatttt ggtcgagatt ggactgtaat tcttccaaac cgtcttcttt gctgataaag
     3601 cggatgttgt cgagccgttt gtcgcgcacc agcaggctgc ggacggtatc gctgtcgctt
     3661 tgtgcggcgg cggtttccat atagacggtg atttgcggcg actcattgag tttgcccaac
     3721 acgctttgcc cgctttggat gcccaagtac ataaacagcg gcagggtcat ggcgacggcg
     3781 agcattatga gggtaagcag tgtgccgaaa ggttggcgca gaagctgttt gagcgcggtg
     3841 cgcgcggatt cgacgtgcag cgagaggtag tggatgatgc tcatgcgagt cgtcctttcg
     3901 agaggcgcag gatgcggtgt ccgtagtccg ccatcagggt ttcgtcatgt gcggcgacga
     3961 tgacggttgt tcccgcttcg tggaaggttt tgaacaattc cataatatcg agcgcgtagg
     4021 cgcggtcgag gttggcggag ggttcgtcgg caatcagcag gctgggctgg tgaacgacgg
     4081 cgcgggcgat gcacaggcgt tgttgttcac cgccggagag ggttacggga tcgtccaatt
     4141 ctcgtccttt caggccgact ttttcgatgg cgatgcgggc acgctcttcg gctttgcgcg
     4201 gcggatagcc gataatccga agcggcagga tgacgttttg caggacgttg cggtcgtaga
     4261 ggattttgtg gtcttggaac acgatgccga tgtgttggcg cataaagccg atttggttgt
     4321 cggacaatgt gccgagatcc tgcccgttaa acaggatttt gcccctgctc ggcttggtga
     4381 tgcccgaaat cagcttgagg acggtggatt tgcccgaacc ggagtgtccc gcgataaata
     4441 tcatttcgcc tttgttgatt tggaagctga cgtttttcag ggcttcaaaa ccgccgggat
     4501 aggttttgga aacttgttcg aaacggatca taggaggtcc tgtaaggaag ggcggcatat
     4561 cgtttcgggc ggttgcgtcc gcagggatat gccgtctgaa caataggtaa gacagtgccg
     4621 attataaaga ttccgcctgt ttttagatag tggataagtt tggcggatac tgaaggtcgg
     4681 gcgggaaaaa tgccgtctga agcggcttca gacggcatcc ggcgtttaac ggcattttga
     4741 atgggcgagt ttgacggctt cggtcaggct tttttcgttc acttccccgg taatggtttg
     4801 tttgtacccg catttcgggg cttcgacgac ggtaaagggc agtacgccga cattgtttcc
     4861 gtaggatttc ataaagtttc ggctgttcgc ctcggtgtaa cgccaaatcg ggtaggaaac
     4921 aggagtttgt ttgaggaagt tgccgatatt gtcggatgtg tcgagcgcga tgccgaccat
     4981 atcgacgctg ccttttttct gcgctttgta ccatttggac atggcgggca tctctttttg
     5041 gcacgggccg caccaggttg cccaaaggtt gacgatgcgt acgggggctt tgagtgattg
     5101 caggctttgt ggggtgttgt ctttccatcc ggccagttcg tctgccgagg cggtatgtgc
     5161 ggcaaatgtg gcggcaaggg tgattgcggc aagattcaag tatttcatcg ttttgtcctt
     5221 tgtatgtcga ttcgggtatt gtaaaccgtt tggtacggtt gtaggcggga tattgagtaa
     5281 aaccctgtgt aagccgataa taaggggatt gtcgagaagc aaggaaatgt catgcctgaa
     5341 atcaaaattt tccacaatcc gcgttgcagc aaatcgcgtg ccgccttgtc cctattggaa
     5401 gaacgcggca ttgctgccga agtggtcaaa tatttggata cgccgcccga cttgtccgaa
     5461 ttgaaggata ttttcaacaa attgggcttg gcatcggcgc gcgggatgat gcgcgtgaaa
     5521 gatgatttgt ataaggaatt gggtttggac aaccccaatt tggacaatga cgcgctgctg
     5581 cgtgccatcg ccgatcatcc cgccctgttg gagcgtccga ttgttttggc aaacggtaag
     5641 gcggctgtcg gcagaccgct ggaaaatatc gaagcggtat tgtgaacaaa cggctgtttt
     5701 gcagccgcaa cggcttgcgc tattacctgt tgggcggttt ctgtttgagt gtttttccgt
     5761 ttttgctggt gtttgcctca tccgtttggg cggtttatcg gacgggcggg caggttttgc
     5821 cgccgtatgt ccgggcggat gccgcgctgg tgttgggtgc cgccgcgtgg gacaaacacc
     5881 cttctccggt ttttcgggaa cgcatcaacc acgccatcgc gctttatcag agccgcaggg
     5941 taggcaaaat cgtgtttacg ggaggacgaa ccaaaaaagg ctatatgacc gaagccgagg
     6001 tcgggcggcg gtacgcgctc aaacaggggg ttccggcgcg caacatcctg tttgaaaaca
     6061 cttcgcgcaa cacttatgaa aacctgaaca acatccgtcc ggttttgcgt gccaacggta
     6121 ttgccagcgt ggtgattgtc agcgatccct accatttggc gcgggcggcg gaaatggcgg
     6181 aggatttggg tgtccgcgtt tatatgtctg ccaccccgac cacgcggttt gatgcgggaa
     6241 ataagaaaaa aatctttatg ctgcaagagg gatatgcgtt atccctctac cgtttggaaa
     6301 aatggggcag ccgctttttg ggatggctgt ctgattgaag cggtagaaaa tgccgtctga
     6361 aacttatccg caggcagggt tcggacggca tttttgatgc ccaagcacgg taaaaccagt
     6421 aaaataaacg ctttcaaaga tttcggagcc atcatgaccg tcaaaacccg tttcgccccc
     6481 agccccaccg gctacctgca cattggcggc gttcgcaccg ccttgttttc gtgggcgttt
     6541 gcccgccatc ataaaggcga atttttattg cgcatcgaag acaccgactt ggcgcgttct
     6601 accgccgaat ccgtcaacat catcctcgac ggcatgaaat gggtcggcct gaactacgac
     6661 aacgccgaca atgtcgtgta ccaaacccgc cgtttcgacc gctacaaaga agtcattgcc
     6721 gaacttttag aaaaaggcca tgcctactac tgctattgca gcaaagaaga gctggaagcg
     6781 atgcgcgaga aagccgaaaa agaaggcacg gcgacttacg accgccgctg gcggccggaa
     6841 gcgggcaaaa ccctgcccga aattcccgcc ggcgtgcaac ccgtcgtccg cttcaaaacg
     6901 cctttggacg gtgtaacccg gtgggcggac ttggtcaaag gcgaaatttc catccccaat
     6961 gaagccctcg acgacctgat tatcgcccga tccgacggct cgccgaccta caacttctgc
     7021 gtcgtggtgg acgactacga catgggcgtt acccacgtta tccggggcga cgaccatgtg
     7081 aacaacaccc cgaaacaaat caacatatta aaagccatcg gcgctaccct gcctgaatac
     7141 ggccacttgc ccatgattct taacgaacaa ggcaaaaaaa tctccaaacg cagcggcgat
     7201 accgttgcca ttaccgattt cggcgcaatg ggcatcctgc ccgaagccat gctcaactat
     7261 ctggcacgct tgggctgggc gcacggcgac gacgagttct ttacgatgga acaattcatc
     7321 gaatggtttg atttgaaaga cgtttccccg tccccaagcc gtatggattt gaaaaagctc
     7381 tattggatca acggcgaaca catcaaaatc acgcccaacg gcaaactcgc cgaactcgtc
     7441 aaaccgcgtc tcgctctgcg cgatatccat gaaactgaaa aacctgcatt ggaagacgtg
     7501 ttggcactgg tcaaagaccg cgcccaagac ctgaacacac ttgccgacga gtgcctctac
     7561 ttctacgtca aacaaacgcc tactgaagcg gacgtgcaga aacactggga cgatgaagcc
     7621 gccgcccgta tgctgcgctt tgccgaacgc ctcgaagggc tggaagactg gaatgccgaa
     7681 gccatccacg acctcttcaa acccttctgc gacgaagaag gcattaaaat gggcaaactc
     7741 ggcatgcccc tgcgccttgc cgtctgcggt acggcgaaaa cccccagcgt cgatgctgtg
     7801 ttggcattaa tcggcaaaga agaagtgttg aaacgcatcc gcgcttaagt tatccacagg
     7861 tgcaggcgat gccgtctgat gtcggaatac ggcttcagac ggcatccaaa cggaagttga
     7921 acatgaaaaa aatattgtat tttctgatgg ttgttttttc tacaagcgta tgggccgggg
     7981 atgcggaaga caatctgctc agcatccaat ccggctatcg tgccttactg caaaagcaaa
     8041 acaatctgga cggaaaaatc atcgggatgc agtcggattt ggaagatgcg cgcagacggt
     8101 tgcaggcggc tcaggcagac atcgcccgtt tggaagcgga aattcctgca gcaatggcgc
     8161 aaaaagcccg gcaggctgaa gagctgaggc aaatcggagt gcgtttggac catgcttgga
     8221 atgcggttta cggcgcaggg ggaacgaagg cgtcggggaa ttgaccgatg aattccctct
     8281 ttgtggacaa tactgttttc attacacggc tgaaagccgg gcatatcggc aggttggttc
     8341 aggcgttgtt tgaggagtgg cacggatttg aaccgtggtc ttctgtggat aagattcgtg
     8401 cctattacgg cagatgtttg aaagatgacg aactgccgct ggcttttgcg gctgtggatg
     8461 atttcggaac cctgttgggt tcggctgcgg tcaagcggca tgatatgaaa agtttcccac
     8521 agtatgaata ttggttgggg gatgtctttg ttttacctga atatcgcgga aaaggcattg
     8581 gcaggaggct ggtcgcccat tgcataggcg cagcgcgttc gctggggata aagttcttgt
     8641 atctttatac gcctgatgtg caaatatttt atgaatcatt cggctgggcg gttgtcgggc
     8701 gacatttcca taacggtgaa tgggttacgg ttatgcgttt ggatatggat aaggtttaag
     8761 ccgaatggac aaatgccgtc tgagctgttc ggacggcatt ttttatattt ggaaatttcc
     8821 tgtcagataa tgtttatcca cagatttttc catatggttt gatgctgttc cgacaacgga
     8881 aattaagata cggatttgca caaatctttc agacggtaca gttctgacaa tgcttcgcgc
     8941 ggggtcaggc tgtcgggatc gagtttttcc aaggctgctg ccaatatacc ttcaaaatgt
     9001 ttttcctctg ctttatccac aaagttgccc acattcggtt catctccttt ttcagacggc
     9061 atggtactga aaatatccag ttggggacgg ttcgcggcgg cttggttttc cagtccgttc
     9121 aaatgctttt gggcggattt caatgcgcgt acaggcaggc cggcgagttt ggcaacggcg
     9181 atgccgtagc ttttgccggc gggaccgggt tggatttggt gcagaaacac gatgtcctgt
     9241 ccctgttcga gcgcggaaag gtgcatattg acggcggcgg cgtgggcttc gggcaggtag
     9301 gtcagctcga aatagtgggt ggcaaacagg ctgaaggatt tgtttttttg cagcaggtgt
     9361 tcggcaacgg cgtgcgcgag ggcgaggccg tcgaaagtgg aagtgccacg tccgacttcg
     9421 tccattaaaa caaggctttg ttcggtggcg tgatgcagga tgtaggcggt ttcgctcatt
     9481 tcgaccatga aagtggagcg gttggaggcg aggtcgtccg atgcgccgat gcgggtgaag
     9541 atttgatcga tgggcccgat tgtggcggca tcggcaggca caaaacagcc ggtgtgtgcc
     9601 aataaaacaa tcagcgcgac ttggcgcatg taggtggatt tgccgcccat attggggccg
     9661 gtgagcagca tgaggcggtg tttgtggtca aggttggtgt ggttggcggt gaagtggcgt
     9721 acctgctgtt cgacaacggg atggcgaccg ttttcgatgt ggatagccgg atagtcggca
     9781 aactcggggc ggacgaagtt ccgctctttt gccaaggctg aaaatgtgga caatacgtcc
     9841 agcgcggcgg cggctttggc ggctttttga agctgcggca atgccgtctg aaggtttttc
     9901 aatacgccgt caaagagttg tttttctaag gcgagggctt gctcttgagc agtcagcact
     9961 ttgtcttcaa aggctttcag ttccggcgtg atgaagcgtt cggcgttttt gagggtttgc
    10021 cggcgttggt agtcggcagg tgcttgttcg gcttgggttt tggacaattc aatgtaaaag
    10081 ccgtgaacgc ggttgaactc gactttgagt gtggacaaac cggtacgttc gcgttccttg
    10141 gcttccaaat ccagcaaaaa ttcgtcgcca tggttttgaa tgcggcgcaa ttcgtccagt
    10201 tcgggatgaa aaccgtggtt gatgacattg ccgtctttca gccagacgga aggttcgggc
    10261 aaaatggctt ggcggagctg ttcggctgtg gataggtttt ccgggaaaac ggctttgagg
    10321 gtttccaaca ggctgctgcc gtttgcggac aagtcgattt gtgccaactc gaacagactg
    10381 tcgcgtaaag atgccaaatc gcgcgggcgg gcgttgccga cggcgatgcg ggcagcaatg
    10441 cgttcaatgt ccgcaatgtt tttcagacgg cattggaggg gtttgtattg gctttccagc
    10501 gcggcaacgg cttcttggcg cgctcggatg tgggcgcggt tgcgtaaagg gtggtgcagc
    10561 cagagagcca agaggcggct gcccatatgg gtagcgcaaa ggtcgagcgt ggacatcagg
    10621 gtcggcgatt ttttgccgga gagggtttgc gtgatttcga gattgcggcg cgtggcagca
    10681 tccataccga tatattggct gtcggtttcg agcgacaggc cgtccaaatg ttgcggcatc
    10741 aggttttgcg tcagacggat atagttcaac agtgcacctg ccgcgccaat cgcaacggcg
    10801 tgttctttgc cgtccaaacc gaagccgcgc aaatcctggc agccgaaata ttccgtcagc
    10861 agtttttccc ccgcgtcggc ggcaaactgc cacgcgttca ggcgcgtaac acccgatgcc
    10921 gtctgaagtt gcggtgcgtt tttactgtcc ggcaacagaa tttccgccgc ctgcaggcgc
    10981 gccagttcgt cgtccaattt atccacagtt gtcagcttgg ttttgaattc gccgctttgc
    11041 agcgatgccc acgccaaacc gatgtatttt ttgtcggggg acacggcaac gatacggttg
    11101 gtttccttgt cttccagcaa tgcggaatcg gtcagcgtgc cgggcgttac gatgcgcacg
    11161 actttgcgct ccacaggccc tttgcccgcg ccgacttcgc cgacctgttc gcaaatcgcc
    11221 acgcttttgc ccaacttgac caggcgcgcc agatattgtt cggcggcgtg aaacggcacg
    11281 cctgccattt tgaccggctc gccatccacc tgtccgcgcg tggtcagggt aatatccaaa
    11341 agttttgccg cttctaccgc atcgtcgaaa aacatctcgt aaaaatcgcc catacggtaa
    11401 aacaccagtt tgtcggtatg ttgcgctttg atgccgaggt attgctgcat cattggggaa
    11461 acggcggatt tggacatggg gctgccttat cattaggaaa acggtattgt agcaaaaggt
    11521 tttgtcttcg acggaatgag actgaccgta agcctgtcaa taaggttgtg gagacaacta
    11581 ggaaaacagc aaagttatcc acggagatat tattaagtca aaaaacatcc acgtttatct
    11641 gtcctatacg gcaagcctta aactcaaact tatttgacga aaaggtaatg aaaatattgg
    11701 atatttgcta cttatacaca agcagatccg caatcatcaa aatcatcatt aaaattttaa
    11761 attcaaatat tgatggtaaa agttttggaa atctgtgcaa acagtaaaat tcatctatat
    11821 caaataagca tcaagtagtt agatgtaata gtggaatgta actttatttc agaatacaat
    11881 acagatattg aaatttcgtt tcacagcttg gagtatatac atgagcatta aagtagcgat
    11941 taacggtttc ggccgcatcg gccgcctcgc attgcgtcaa attgaaaaag cccatggcat
    12001 cgaagtcgtc gctgtcaacg acctgacccc tgccgaaatg ctgctgcacc ttttcaaata
    12061 cgacagcacg caaggccgtt tccaaggtac tgccgaatta aaagacgatg ccatcgtggt
    12121 aaacggcaaa gaaatcaaag ttttcgccaa tccgaatccc gaagaattgc cttggggcga
    12181 gttgggtgtg gatgtcgtcc tcgaatgtac cggcttcttt accaataaaa ccaaagccga
    12241 agcacacatc agggccggcg cgcgcaaagt cgtgatttcc gcacccggcg gcaatgatgt
    12301 gaaaaccgtc gtatatggcg taaaccaaga cattttggac ggcagcgaaa ccgttatctc
    12361 cgctgcttcc tgcaccacca actgccttgc cccgatggcg gcagtcctgc aaaaggaatt
    12421 cggcgtagtc gaaggcctga tgaccaccat ccacgcctac accggcgacc aaaacaccct
    12481 tgacgcgccg caccgcaaag gcgatttgcg ccgcgcccgc gccgccgcgc tcaacatcgt
    12541 gcccaacagc accggtgccg ccaaggccat cggtctggtt atccctgaat tgaacggcaa
    12601 actcgacggc tccgcccaac gcgtccctgt tgccaccggc tcgctgaccg aattggtttc
    12661 cgtcctcgaa cgccctgtaa ccaaagaaga aatcaacgcc gcgatgaaag ccgccgccag
    12721 cgaatcttac ggctacaacg aagatcaaat tgtttcttcc gacgttgtcg gcatcgaata
    12781 cggttcgctc ttcgatgcga cccaaacccg cgtgatgacc gtgggcggca aacaattggt
    12841 gaaaactgtt gcctggtacg acaacgaaat gtcctacacc tgccaactcg tccgcacttt
    12901 ggaatacttc gcaggcaaaa tctaaggctt gaacaaacct gtggataagt aaatgccgtc
    12961 tgaacacatt gcgttcagac ggcattgttt atctgtat
//
LOCUS       DAWKQB010000047        12867 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.047, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000047 DAWKQB010000000
VERSION     DAWKQB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.047"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..134)
                     /locus_tag="WJL14_000984"
     CDS             complement(<1..134)
                     /locus_tag="WJL14_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ7656072.1"
                     /translation="MAFTLMRRAMIRKMPYTEDMRPGDTANPYGASKAMVERMLTDIQ
                     "
     gene            complement(177..521)
                     /locus_tag="WJL14_000985"
     CDS             complement(177..521)
                     /locus_tag="WJL14_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ7656073.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(514..1516)
                     /gene="dcm"
                     /locus_tag="WJL14_000986"
                     /pseudo
     CDS             complement(514..1516)
                     /gene="dcm"
                     /locus_tag="WJL14_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1547..3820)
                     /locus_tag="WJL14_000987"
     CDS             complement(1547..3820)
                     /locus_tag="WJL14_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ7656074.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3884..4534)
                     /locus_tag="WJL14_000988"
     CDS             complement(3884..4534)
                     /locus_tag="WJL14_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656075.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4531..5328)
                     /locus_tag="WJL14_000989"
     CDS             complement(4531..5328)
                     /locus_tag="WJL14_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7656076.1"
                     /translation="MKELHKTSFLESLLIQKRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLLTLVMVLMWKFFRMHNVSALNIVAFTLTGYPMMMMWRNASNHAIGSISANTSLL
                     YHRNVRVLDTIFARMLLEIAGATIAQVVIMFALVIIGWIDVPADIFYMLLAWLLMAMF
                     AVGLGLVICSVAFHFEPFGKVWSTISFVMMPLSGVFFFVHNLPQQLQHYVLMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5328..6491)
                     /locus_tag="WJL14_000990"
     CDS             complement(5328..6491)
                     /locus_tag="WJL14_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export inner-membrane
                     protein CtrB"
                     /protein_id="HEZ7656077.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVQEYMQSR
                     SALDALRKKMPIRDFYEKEGDIFSRFNGFGLRGEDEAFYQYYRDKVSIHFDSVSGISN
                     LSVTSFNAGESQKINDALLKQGEVLINQLNERARQDTIRYAQEVVNSAEEQVKEASVQ
                     LTKFRVSNGIFDLKAQSDVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLIARE
                     KSLRKEISQQMKAISGGGEGSLSNQAAEYQRVYLENELAEKQLAAAMTSLESAKAEAD
                     RQQLYLEVISQPNKPDLAYEPNRLYNIVATFVIGLIVYGIVVLLSASIREHKN"
     gene            complement(6506..7669)
                     /gene="ctrA"
                     /locus_tag="WJL14_000991"
     CDS             complement(6506..7669)
                     /gene="ctrA"
                     /locus_tag="WJL14_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ7656078.1"
                     /translation="MLKLKFCIVISFLILGSACSAIPSSGPSAKKVVSLGQQSEAQIP
                     EVELIDVNHAVAQSLYKAQVNQSFTQFGDGYASTGTLNIGDVLDIMIWEAPPAVLFGG
                     GLSSMGSGSAQQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKKMANQ
                     PQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNVQLTR
                     GNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSARGLSL
                     SEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVYRVNM
                     ADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7888..9006
                     /gene="wecB"
                     /locus_tag="WJL14_000992"
     CDS             7888..9006
                     /gene="wecB"
                     /locus_tag="WJL14_000992"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEZ7656079.1"
                     /translation="MKVLTVFGTRPEAIKMAPVILELQKHNTITSKVCITAQHREMLD
                     QVLSLFEIKADYDLNIMKPNQSLQEITTNIISSLTDVLEDFKPDCVLVHGDTTTTFAA
                     SLAAFYQKIPVGHIEAGLRTYNLYSPWPEEANRRLTSVLSQWHFAPTEDSKNNLLSES
                     IPSDKVIVTGNTVIDALMVSLEKLKITTIKKQMEQAFPFIQDNSKVILITAHRRENHG
                     EGIKNIGLSILELAKKYPTFSFVIPLHLNPNVRKPIQDLLSSVHNVHLIEPQEYLPFV
                     YLMSKSHIILSDSGGIQEEAPSLGKPVLVLRDTTERPEAVAAGTVKLVGSETQNIIES
                     FTQLIEYPEYYEKMANIENPYGIGNASKIIVETLLKNR"
     gene            9008..10645
                     /locus_tag="WJL14_000993"
     CDS             9008..10645
                     /locus_tag="WJL14_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide phosphotransferase SacB"
                     /protein_id="HEZ7656080.1"
                     /translation="MFILNNRKWRKLKRDPSAFFRDSKFNFLRYFSAKKFAKNFKNSS
                     HIHKTNISKAQSNISSTLKQNRKQDMLIPINFFNFEYIVKKLNNQNAIGVYILPSNLT
                     LKPALCILESHKEDFLNKFLLTISSENLKLQYKFNGQIKNPKSVNEIWTDLFSIAHVD
                     MKLSTDRTLSSSISQFWFRLEFCKEDKDFILFPTANRYSRKLWKHSIKNNQLFKEGIR
                     NYSEISSLPYEEDHNFDIDLVFTWVNSEDKNWQELYKKYKPDFNSDATSTSRFLSRDE
                     LKFALRSWEMNGSFIRKIFIVSNCAPPAWLDLNNPKIQWVYHEEIMPQSALPTFSSHA
                     IETSLHHIPGISNYFIYSNDDFLLTKPLNKDNFFYSNGIAKLRLEAWGNVNGECTEGE
                     PDYLNGARNANTLLEKEFKKFTTKLHTHSPQSMRTDILFEMEKKYPEEFNRTLHNKFR
                     SLDDIAVTGYLYHHYALLSGRALQSSDKTELVQQNHDFKKKLNNVVTLTKERNFDKLP
                     LSVCINDGADSHLNEEWNVQVIKFLETLFPLPSSFEK"
     gene            10718..11461
                     /locus_tag="WJL14_000994"
     CDS             10718..11461
                     /locus_tag="WJL14_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7656081.1"
                     /translation="MLSNLKTGNNILGLPEFELNGCRFLYKKGIEKTIITFSAFPPKD
                     IAQKYNYIKDFLSSNYTFLAFLDTKYPEDDARGTYYITNELDNGYLQTIHCIIQLLSN
                     TNQEDTYLLGSSKGGVGALLLGLTYNYPNIIINAPQAKLADYIKTRSKTILSYMLGTS
                     KRFQDINYDYINDFLLSKIKTCDSSLKWNIHITCGKDDSYHLNELEILKNEFNIKAIT
                     IKTKLISGGHDNEAIAHYREYFKTIIQNI"
     gene            11463..12326
                     /locus_tag="WJL14_000995"
     CDS             11463..12326
                     /locus_tag="WJL14_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEZ7656082.1"
                     /translation="MRKITFIIPIKQSLIKPDCFIRLFFNLFLLKKFSSKYGFSILVA
                     DNSNFLWKNIIKLITKFYKCNYISIKSHNTFYTPAKIKNAAAIYSFNTLNSNYILFLD
                     VDVLLSENFIQHLIKKTKTNIAFDWYPVSFLNKQFGIINFILFSYKGNLNIEESFIIQ
                     TGFVTGLQLFNSDFFYKTAGYNESFLGYGCEDIEMIHRATLLLNIRPAFNENHQYFTD
                     DRGYMPSKLTGFRNYFYYLKRDEFSNLQITPKHFWHKRKNKSKYLKNRYQNDVKMIQI
                     MKDFDRKFLKN"
     gene            12371..>12867
                     /locus_tag="WJL14_000996"
     CDS             12371..>12867
                     /locus_tag="WJL14_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7656083.1"
                     /translation="MKNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYGTSKSMVERI
                     LTDIQ"
BASE COUNT         3886 a         2872 c         2493 g         3616 t
ORIGIN      
        1 ttttggatgt cggttaacat ccgttccacc atcgctttgg atgcgccata aggattagcg
       61 gtatcgcccg ggcgcatatc ttccgtatag ggcattttac gtatcatggc gcgacgcatt
      121 aaagtaaacg ccaaagcgcg tttatactgg ttatccagtt tccctatatt tttacgttac
      181 aaatcaagtt gttggtattc atcgtgaaaa tagaattttt cggcataacg ctgactcata
      241 aaaccggcat gcgggctgcc tgaaaaaatc attgcgacat catcgtcatt taatgtttca
      301 tggttgtttt ctattaatgc taaagcaatt tggtaattaa ttgccaaaat atcgttagta
      361 atcacacgat agccgcgttt tttggcttcg taggcaaagg aacagcctcc actaaataca
      421 tctgcaactg tatctacatc agacggaagc tggtcacaaa tccaggaagc tattttctct
      481 ttattaccga tatagttaat tttcggatat tgcttattca ttttcactca tttttaaaat
      541 actttcagca atagcttttg ccaataaagg cggtacggcg ttaccaacct gctgctgctg
      601 ggcaattttg ctgccgcaaa aaataaaatt atcagggaaa gattgtaagg cagctaattc
      661 acgaacggtt aacgcccgat tctgttcata gtgaaaaact ttgcgcatat ctcctgtaat
      721 acaaacggct ggttttgttg ctgttgtaac ggatgtattt acggatatca cctgttttcg
      781 gacgtaatgg ttcaggaata tcgttacggt tacctccatt tttaacaaat gccatttttt
      841 ctaacatttg tgccgaatga ttcatagctt catgatttgc aacgtgtgga ttgctttcgc
      901 cagcagccag ttttggaaaa tgtcctattg ctgatccaac agtctgatgg gaaatctgca
      961 aaggttcggg aaaggaaatt ttgcctttat ccctcctccc gataaatatc actcggctac
     1021 gtatctgagg aacaccgaaa tcggctgcac tcagtatctt acattccacc gaataaccga
     1081 tattctgaaa tgcttgaata atctcaatac gtgttttacc tgaattgtgt gtatagagtc
     1141 gcgctacatt ttccataaca aaaaaatatg gttggacaat tttaactatt cggacaaact
     1201 ctttaaataa atggttgcgt gggtcatctg taaatgtccg tccaatcttt cctgccatac
     1261 taaaaccttg acaaggtggt cctccaataa tcaaatcaac tgcttgtccg ttaagacaat
     1321 tgattaaatc ttgttcggtt agtgtggtta aatctttttg cagtaattga tgatggggga
     1381 agttggtacg gtaagtctga caataatcag actccatttc aacagaaagc aattgttgga
     1441 atccggcttg ttcaaaaccc aaggataggc ctcctgctcc tgagaaaagg tcaatatagg
     1501 taagtggtgt gtgcataagt caaaatccat aaactcttcg attattttac cgcttcagct
     1561 tcgcaaacgc atccgccatc gccgaattgg ctggggcgcg gtcgttgcgt tggggtttgc
     1621 ggccggctgt ccgttcgcgg ctgcggtttt cagacggcat tttatgtttt gcgccgcccg
     1681 gttcgtcatc caagcgcatg gtcagcgcga tgcgtttgcg tgcagcatcg acttccagca
     1741 ctttcacttt caccacgtcg ccagctttca ccacttcgcg cgggtcttgg acgaacttgt
     1801 tggacagggc ggagatgtgc accaagccgt cctgatggac gccgatgtcc acgaacgcgc
     1861 cgaagttggc gacgttggaa accacgcctt cgagtatcat accgacttgc aagtcgctga
     1921 tttcgtggat accttcggca aacgatgccg tctgaaactc gccgcgcgga tcacggccgg
     1981 gtttttccag ttcggacagg atgtccaaaa tggtcggcag gccgaagcgt tcgtcggtga
     2041 agtcggacgc tttgatttgc ttcacgcgct cgcggttgcc gatgagttcg gcggcgctaa
     2101 tgccttgttg cgccagcatt ttggcgacga cgggataggc ttcggggtgg acggcgctcg
     2161 cgtccaacgg ctctttaccg ccgttaatcc gcaaaaagcc tgccgcctgc tcgaaggttt
     2221 tttcgcccaa acgcggtact ttcagcaatt ttttgcggct gtcgaacgcg ccgttttcat
     2281 cgcggtaggc aacgatgttt tgggcaaggg tttgattcaa accggaaatt cgcgccaaga
     2341 gcggggcgga ggcggtgttc acgtccacgc cgacggcgtt cacgcagtct tcgaccaccg
     2401 cgtccagcga tttggcgagc tggctttggt tcacgtcgtg ctgatactgc cccacgccga
     2461 tggatttggg gtcgattttg accaactcgg caagcgggtc ttgtagcctg cgggcgatgg
     2521 acaccgcgcc gcgcagggaa acgtccaagt cggggaactc gcgcgccgcc agttcggacg
     2581 cggaataaat cgacgcgccg gcttcggaca cgacgatttt atgcagcccc attttcgaca
     2641 tttcacgcac cagttcgccc gcgattttgt cggtttcacg gctggcggtg ccgttaccga
     2701 tggcgatgag cttcacgccg tgctgtttaa tcaggcgcga cagcgttgcc aacatattgt
     2761 tttcttgatg caaatagacg atgacggtat ccagcagctt gccggtgtcg tccaccacgg
     2821 cgcatttcac gccgttgcgg tagccggggt cgagacccag cgtggtcagc cgtccggcgg
     2881 gcgcagcaag cagcaagtct ttgagattgc gggcgaacac ggtaatcgcg tcggtgtcgg
     2941 cggcttcttt caaacgattg agagcttcaa gttccaacga caaaaagatt ttcgcgcgcc
     3001 aagtcaggcg cacggtgtcg cgcagccatt tgtggccgtc tgaaactttg aagcggcggg
     3061 cgatgatttg ctcgtattcg ctttgctgcg taatcggcgt gtcgtcgggc tgatatttga
     3121 gcgcgatgtt caacacgcct tcgttgcgtc cgcgcaaaac cgctagcgca cgatggctgg
     3181 gcatcgcacg aataggttcg cggtggtcaa aataatcgct gaatttttcg ccttcggttt
     3241 ctttgcctcc aacaacttgc gcgtggattt cggcttcgtt ccacagcttg tcgcgcagcg
     3301 tgccgatgag ttccgcgtct tcggcaaact gctccatcag aatcgcacgc gcgccgtcca
     3361 acgcggcttt ggcatcgggg acgttttcgt tcaggtagcc ttgtgcggcg gcttccacgt
     3421 cctgcgactg ctcggcaagc aacacgtccg ccagcggctg caaaccgtgt tcgcgcgcga
     3481 tttgcgcttt ggtgcggcgt ttgggtttgt agggcagata caggtcttcc agcgcggttt
     3541 tgttatcggc ggcttcgatt tgcgccctga ggtcgtctga aagcttgcct tgctcttcaa
     3601 tgctttttaa aacaacggct ttgcgctctt ccaactcgcg cagatattgc agccgctcgg
     3661 caagccggcg caactgcgta tcgtccaacc cgcccgtcgc ttccttgcgg tagcgggcga
     3721 taaacggcac ggtcgcgccg tcgtccaaaa gctcgacggc ggcggtgatt tgcgcggcag
     3781 tcgcggagag ttcttgggaa agaatttgag taatgttcat caataaaatt ccaacggaca
     3841 ggccgtctga aatttcagac ggcctgattt aaaaacaatc gctttaaggc agcgaattat
     3901 aatattcgta ggctttgtcc atatcttcaa actggtacat atgccctttt tccagcacca
     3961 tcgcattatc gcaatattgc ttcatggcgc tgtggctgtg cgacaccaag atgatggaac
     4021 ggtctttgcg cttttcaaac aactcgtact tacatttatc ggcaaaacgc gagtcaccaa
     4081 ctgcaatcac ttcgtcaatc aaatagcaat caaactctac cgccaacgac aaagcaaatg
     4141 ccaaacgcgc cttcatacct gaagaataac gcttgaccgg ctcgtaaaga tattgcccca
     4201 actcggaaaa ctcttcggta aagttcttca cataatcgat atcgacattg taaatccggc
     4261 agatgaaacg caaattgtcc ataccggtca gactgccttg aaacgcaccg gagaatgcca
     4321 aaggccaaga aatactcatt gtccgcttga tttcacccgt ggtcggcggc tcaacgccac
     4381 tgatcaaacg gatgagcgtc gatttacctg caccgttgcg gccgagaata ccgattttct
     4441 cgcccttctc cattttgaag ctaatatcgt gcaagactgt ccgccaacct tggcgggtca
     4501 gatagcgttt ggaaacgtgt tcaactgaaa tcattgcggc tcaacccctt tgctgaatct
     4561 cgccaccacc gccaaaccaa gcagcagcaa aaccaagtta cacaacaaaa tataccaagg
     4621 attttcgtaa gtcgtgacgc tgtcgccaaa atatccggca cggaacattt ctgtaccatg
     4681 caccatcgga atcatcagca catagtgttg caactgctga ggcaggttgt gtacgaagaa
     4741 aaacacgccg gacaaaggca tcatcacaaa actgatggta ctccaaactt tgccaaacgg
     4801 ttcaaaatga aacgccaccg aacagatgac cagccccaat ccaacggcaa acatagccat
     4861 caaaagccaa gccaacaaca tatagaaaat atctgccggt acatcaatcc aaccgatgat
     4921 aaccaatgcg aacataatca cgacctgcgc aatggtggcg ccggcaatct ccaacaacat
     4981 acgcgcaaaa atggtatcca atacgcgtac atttcggtga tacagcaggc ttgtgttggc
     5041 agaaatcgaa ccgatagcat gattagaagc attacgccac atcatcatca tcggatagcc
     5101 tgtcagcgtg aatgccacga tattcaaagc tgacacgttg tgcatacgga aaaacttcca
     5161 catcaacacc ataaccaaag tcagcagcaa aggttcgaca aacaaccaca aaaaaccaat
     5221 gttattacga ccgtagcgag taataatttc acgcattaag agtgcgccga ttaccctttt
     5281 ttggataagc agcgactcca aaaacgatgt tttatgtagc tctttcatca gtttttatgc
     5341 tcacggatac ttgcagacag caacacgaca atgccataga caatcaaacc gatgacaaac
     5401 gttgccacga tgttgtacaa acggtttggc tcatatgcca agtcaggttt gttcggctgg
     5461 gaaatcactt ccaaatacaa ttgctgacga tctgcttccg cttttgcact ttccaaagaa
     5521 gtcatcgccg cagccaattg tttttcggcc aattcgtttt ccaaatacac gcgttgatat
     5581 tccgccgcct gattggacaa cgaaccctcg ccaccgccgg aaatcgcttt catttgttgc
     5641 gagatttctt tacgcaaact cttctcacgc gcaatcaaac ctggaatttg cgggttttca
     5701 ggcgtaacgg cttttacctg gtccagctgg gtctgaatga caatcaactc gtcctgtaat
     5761 ttggatacca aacccatttg cacatcggat tgcgccttca agtcgaaaat accgttagac
     5821 acacggaatt ttgtcaattg gacagaagcc tctttaactt gctcttcagc agaattgacc
     5881 acttcctgag cataacgaat cgtatcctga cgcgcacgtt cgttcagctg gttaatcaat
     5941 acctcaccct gtttaagcaa agcatcattg atcttttgag attcaccggc attaaacgat
     6001 gtaacgctca aattggaaat gcctgagaca gagtcaaaat ggatggatac cttatcacgg
     6061 tagtattgat aaaacgcctc atcctcgcca cgcaggccaa aaccattaaa acggctgaaa
     6121 atatcgcctt ctttttcata aaaatcgcga atgggcattt tcttacgcaa cgcatccaaa
     6181 gccgaacgcg actgcatata ttcctgcacg gtataaatat cgtcttgcgc acgggcaaaa
     6241 cctgtgccct gcaaaatggc acccaggcca ttgagagaag attggctttt aggcgagcgc
     6301 accacaaagc tcgattgcga cgtaaaacga tcggaagcga agaagccgaa atacaccaac
     6361 gaaattaccg tagggataat caccgttacc caaaataaag ggcttagctt tttaatccaa
     6421 cttttctttt tcggcttttt acgctcggct ttggtttcag tggcaactgc cacaggaagt
     6481 tgctcagaca tcttaattac tcacattaat tagttaaatt attaatactg ttcgcgccac
     6541 tggtaaccgg cgagaacaca aacgacaaga atttctgcac ttcagccaac ggcgcattcg
     6601 acacatacaa tacatcttta ttcttcacag gaaagcgctg catagaaaat agcgaatgcg
     6661 catcagccat attcacacga tataccgttg gaatctctgc ctcactgcca taaccttgag
     6721 caatccattt atcctgacgt tctgccggca attccaccaa tggcgtatag cggaacacaa
     6781 acacaccacg cgcatcagaa cggcgatctt gcaaaccgcc catacggcca atggcttcag
     6841 aaagcgataa gcctctagct gaaaaaccga tttcttgtgt tctccccacc gcacccatag
     6901 acgtaaaggt atagggattg gtaatcatgg taaccacatc accgcgacgc agcaaaatat
     6961 tttgtcgcgg atttgcaact aaatcttcca aggcaacagt tcgtactaca ttgccacgtg
     7021 tcagctgcac attcgtatcc tgcacatttg ccgttgaacc acctaccgca gccaccgcat
     7081 ccaacacacg ctcaccggct gccgtcaacg gcatacgcac actattgcct gcgcgaatca
     7141 ccgatacatt tgccgcatta ttctgcacca agcgcaccat cacttgcggc tgattggcca
     7201 tttttttcag gcggccttta ataatttcct gaacctgacc aggcgtttta ccgaccaccg
     7261 aaatatcgcc aacaaacggc acagaaaccg taccacgtgc cgtcaccagt tgctccggca
     7321 acttggtttg ttgcgcacta cccgagccca tcgaagaaag gccaccacca aacaataccg
     7381 ctggcggcgc ttcccaaatc ataatatcca atacatcacc aatatttagc gtaccggtag
     7441 aagcataacc atcgccaaac tgggtaaaag actgattcac ctgagcttta tacaaagact
     7501 gcgctacagc atgattgacg tcaatcaact ctacttcagg aatttgagct tcagactgtt
     7561 gccccaatga gacaactttt tttgcacttg ggccagagga gggaatagca ctacatgcac
     7621 ttcccaaaat taaaaaagaa attacaatac aaaactttaa cttaagcata aaataaaaaa
     7681 tctcattaag tatgattgtt tttaaataaa tttaaaacct accagagata caataccact
     7741 ttattttgta gaacacaaac gtgtataata tataacataa acatcatctt cgaaataata
     7801 ttggggctta ggaagcaaaa tcatcaaaaa acgtgataag ctcctaatat ttttaacaca
     7861 ttactatatt acacatagga tattccaatg aaagtcttaa ccgtctttgg cactcgccct
     7921 gaagctatta aaatggcgcc tgtaattcta gagttacaaa aacataacac aattacttca
     7981 aaagtttgca ttactgcaca gcatcgtgaa atgctagatc aggttttgag cctattcgaa
     8041 atcaaagctg attatgattt aaatatcatg aaacccaacc agagcctaca agaaatcaca
     8101 acaaatatca tctcaagcct taccgatgtt cttgaagatt tcaaacctga ctgcgtcctt
     8161 gttcacggag acaccacaac aacttttgca gctagccttg ctgcattcta tcaaaaaata
     8221 cctgttggcc acattgaagc aggcctgaga acttataatt tatactctcc ttggccagag
     8281 gaagcaaata ggcgtttaac aagcgttcta agccagtggc attttgcacc tactgaagat
     8341 tctaaaaata acttactatc tgaatcaata ccttctgaca aagttattgt tactggaaat
     8401 actgtcatag atgcactaat ggtatctcta gaaaaactaa aaataactac aattaaaaaa
     8461 caaatggaac aagcttttcc atttattcag gacaactcta aagtaatttt aattaccgct
     8521 catagaagag aaaatcatgg ggaaggtatt aaaaatattg gactttctat cttagaatta
     8581 gctaaaaaat acccaacatt ctcttttgtg attccgctcc atttaaatcc taacgttaga
     8641 aaaccaattc aagatttatt atcctctgtg cacaatgttc atcttattga gccacaagaa
     8701 tacttaccat tcgtatattt aatgtctaaa agccatataa tattaagtga ttcaggcggc
     8761 atacaagaag aagctccatc cctaggaaaa ccagttcttg tattaagaga tactacagaa
     8821 cgtcctgaag ctgtagctgc aggaactgta aaattagtag gttctgaaac tcaaaatatt
     8881 attgagagct ttacacaact aattgaatac cctgaatatt atgaaaaaat ggctaatatt
     8941 gaaaaccctt acgggatagg taatgcctca aaaatcattg tagaaacttt attaaagaat
     9001 agataaaatg tttatactta ataacagaaa atggcgtaaa cttaaaagag accctagcgc
     9061 tttctttcga gatagtaaat ttaacttttt aagatatttt tctgctaaaa aatttgcaaa
     9121 gaattttaaa aattcatcac atatccataa aactaatata agtaaagctc aatcaaatat
     9181 ttcttcaacc ttaaaacaaa atcggaaaca agatatgtta attcctatta atttttttaa
     9241 ttttgaatat atagttaaaa aacttaacaa tcaaaacgca ataggtgtat atattcttcc
     9301 ttctaatctt actcttaagc ctgcattatg tattctagaa tcacataaag aagacttttt
     9361 aaataaattt cttcttacta tttcctctga aaatttaaag cttcaataca aatttaatgg
     9421 acaaataaaa aatcctaagt ccgtaaatga aatttggaca gatttattta gcattgctca
     9481 tgttgacatg aaactcagca cagatagaac tttaagttca tctatatctc aattttggtt
     9541 cagattagag ttctgtaaag aagataagga ttttatctta tttcctacag ctaacagata
     9601 ttctagaaaa ctttggaagc actctattaa aaataatcaa ttatttaaag aaggcatacg
     9661 aaactattca gaaatatctt cattacccta tgaagaagat cataattttg atattgattt
     9721 agtatttact tgggtcaact cagaagataa gaattggcaa gagttatata aaaaatataa
     9781 gcccgacttt aatagcgatg caaccagtac atcaagattc cttagtagag atgaattaaa
     9841 attcgcatta cgctcttggg aaatgaatgg atccttcatt cgaaaaattt ttattgtctc
     9901 taattgtgct cccccagcat ggctagattt aaataaccct aaaattcaat gggtatatca
     9961 cgaagaaatt atgccacaaa gtgcccttcc tacttttagc tcacatgcta ttgaaaccag
    10021 cttgcaccat ataccaggaa ttagtaacta ttttatttac agcaatgacg acttcctatt
    10081 aactaaacca ttgaataaag acaatttctt ctattcgaat ggtattgcaa agttaagatt
    10141 agaagcatgg ggaaatgtta atggtgaatg tactgaagga gaacctgact acttaaatgg
    10201 tgctcgcaat gcgaacactc tcttagaaaa ggaatttaaa aaatttacta ctaaactaca
    10261 tactcactcc cctcaatcca tgagaactga tattttattt gagatggaaa aaaaatatcc
    10321 agaagagttt aatagaacac tacataataa attccgatct ttagatgata ttgcagtaac
    10381 gggctatctc tatcatcatt atgccctact ctctggacga gcactacaaa gttctgacaa
    10441 gacggaactt gtacagcaaa atcatgattt caaaaagaaa ctaaataatg tagtgacctt
    10501 aactaaagaa aggaattttg acaaacttcc tttgagcgta tgtatcaacg atggtgctga
    10561 tagtcacttg aatgaagaat ggaatgttca agttattaag ttcttagaaa ctcttttccc
    10621 attaccatca tcatttgaga aataagttaa attatgaaga acctttgagt gcaattcgaa
    10681 ggtccttcat tcatattatt catattttgg agaaattatg ttatctaatt taaaaacagg
    10741 aaataatatc ttaggattac ctgaatttga gttgaatggc tgccgattct tatataaaaa
    10801 aggtatagaa aaaacaatta ttactttttc agcatttcct cctaaagata ttgctcaaaa
    10861 atataattat ataaaagatt ttttaagttc taattatact tttttagcat tcttagatac
    10921 caaatatcca gaagatgatg ctagaggcac ttattacatt actaatgagt tagataatgg
    10981 atatttacaa accatacatt gtattattca attattatcg aatacaaatc aagaagatac
    11041 ctaccttttg ggttcaagta aaggtggcgt tggcgcactt ctactcggtc ttacatataa
    11101 ttatcctaat ataattatta atgctcctca agccaaatta gcagattata tcaaaacacg
    11161 ctcgaaaacc attctttcat atatgcttgg aacctctaaa agatttcaag atattaatta
    11221 cgattatatc aatgacttct tactatctaa aattaagact tgcgactcct cacttaaatg
    11281 gaatattcat ataacttgcg gaaaagatga ttcatatcat ttaaatgaat tagaaattct
    11341 aaaaaatgaa tttaatataa aagctattac gattaaaacc aaactaattt ctggcgggca
    11401 tgataatgaa gcaattgccc actatagaga atactttaaa accataatcc aaaatatata
    11461 aaatgcgtaa gattactttt attatcccta taaaacagtc tttaataaaa cctgattgct
    11521 ttatacgcct cttttttaat ttatttttgc taaaaaaatt ctcaagtaaa tacggatttt
    11581 ctatattagt tgcagacaac agtaacttcc tttggaaaaa tattattaaa ttaattacaa
    11641 aattttacaa atgtaattat attagtatta aatctcataa tactttttat acgcctgcta
    11701 aaattaaaaa tgcagctgcc atctatagtt ttaatacctt gaattcaaat tacattttat
    11761 tcttagatgt tgacgtttta ttatcggaaa attttatcca acatttaata aaaaaaacaa
    11821 aaaccaatat cgcctttgat tggtaccctg tttcattctt aaacaaacaa tttgggatta
    11881 taaattttat attattctca tataaaggta atctaaatat agaagaatca ttcattatac
    11941 aaacagggtt tgtaactggc ttacaattat ttaattctga ttttttctac aaaacagctg
    12001 gatacaatga aagctttctt ggctatggct gtgaagatat tgaaatgatt cacagagcaa
    12061 cattattatt aaatattaga cctgccttta atgaaaatca tcaatatttt acagatgata
    12121 gaggatatat gccttctaaa ttaaccggat ttcgaaatta tttttattat ttgaaaagag
    12181 atgaattttc aaacttacag ataactccta aacatttctg gcataagcga aaaaataaat
    12241 caaaatatct aaaaaataga tatcaaaatg atgtaaaaat gattcagatt atgaaagatt
    12301 ttgatcgaaa atttctaaaa aattaacgag ctgtcttgcc catatgaatc ctgattactt
    12361 taatttaatt atgaaaaata ttctcgttac cggcggcacc ggttttatcg gctcgcacac
    12421 cgttgtttct ttgctgaaaa gcggccatca agtcgtgatt ttggataacc tatgcaattc
    12481 cagcatcaat atcctgccac gcttgaaaac gattaccggc caagagattc cgttttatca
    12541 aggcgatatc cgcgatcgtg agattttgcg ccgtattttt gcggaaaacc gcattgattc
    12601 ggtgattcat tttgccggct tgaaagcggt gggtgaaagt gtggccgagc cgatgaaata
    12661 ttatgataat aatgtttccg gcagcttggt gttggcggaa gaaatggcgc gtgcgggcgt
    12721 gtttagcatt gtgttcagtt cttcggcgac ggtttatggc gatccgggca aagtgcctta
    12781 taccgaggat atgccactgg gcgataccac cagcccttac ggcacatcga aatcaatggt
    12841 tgagcgcatt ctcactgaca ttcaaaa
//
LOCUS       DAWKQB010000048        12865 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.048, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000048 DAWKQB010000000
VERSION     DAWKQB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.048"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(53..361)
                     /locus_tag="WJL14_000997"
     CDS             complement(53..361)
                     /locus_tag="WJL14_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656084.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDALKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="WJL14_000998"
     CDS             complement(345..647)
                     /locus_tag="WJL14_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ7656085.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /locus_tag="WJL14_000999"
     CDS             complement(721..3084)
                     /locus_tag="WJL14_000999"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ7656086.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDVGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIRKMPSEGGKTRAVKIDAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEVKQVGLRLGRLKTVLGVDIPSEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVNEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGYPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGM
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="WJL14_001000"
     CDS             complement(3120..3638)
                     /locus_tag="WJL14_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656087.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="WJL14_001001"
     CDS             complement(3645..4709)
                     /locus_tag="WJL14_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7656088.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJL14_001002"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="WJL14_001002"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7656089.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPVRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WJL14_001003"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="WJL14_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ7656090.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WJL14_001004"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="WJL14_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ7656091.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WJL14_001005"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="WJL14_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ7656092.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WJL14_001006"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="WJL14_001006"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ7656093.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGAGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWENYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEQRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQLVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="WJL14_001007"
     tRNA            complement(9446..9522)
                     /locus_tag="WJL14_001007"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="WJL14_001008"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="WJL14_001008"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ7656094.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQAQFHENRARGTD"
     gene            10826..11908
                     /locus_tag="WJL14_001009"
     CDS             10826..11908
                     /locus_tag="WJL14_001009"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ7656095.1"
                     /translation="MPSEAASDGILPRYPTRTIFQKPIMLPFLPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12099..12567
                     /locus_tag="WJL14_001010"
                     /pseudo
     CDS             12099..12567
                     /locus_tag="WJL14_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692723.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
     gene            complement(12564..12854)
                     /locus_tag="WJL14_001011"
     CDS             complement(12564..12854)
                     /locus_tag="WJL14_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656096.1"
                     /translation="MSKYVTETDIGVREHFDFFGEFVVSPAARSDDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3288 a         3385 c         2843 g         3349 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagagcgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accagaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccatgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgactttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggcg ggatatccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttgatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtta acaaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc ggcctcaatt tcaatgtcga aacggaagct
     1741 cggcgcagta acgcggaagc cttccgccgt tttctcgggc tgcaaaccca aatgttgcaa
     1801 aatggtttcc acctgttcgg agggaatgtc cacgcccaac acggttttca gacgacccaa
     1861 acgcaatccg acctgcttga cttcaggcaa ttcgccttgc gcttccacca tttctcctgc
     1921 cgcaccgccg caaatctgca aaaccaattc ggtggcgcgt tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acgcgccttt
     2161 ttcgtctgcc acgaccagcg tattttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 gaaaacgtgc atcggctggc cgatttccag catcacataa ttgccgatgt ccaccagcac
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatcgg gagtagccgc
     2401 tttcgcattg acattttcaa taacgcggct gataaaacgg ccgcaatcgg caggcgcgtc
     2461 aattttcaca gcccgggttt taccgccttc agacggcatt ttccggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cccttaacac tcaagcaatc
     2581 agcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcacg
     2641 gatattggta ccgacgggcg cgtcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgttc gacaccacac cgcgcatttt
     2761 agtcggcttg attttgaaat tgcccggcaa caccgcaccc ggcagcgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaactcac ccgtgcccac
     2881 atcgactcgg gtaacgttca aacggtcggc atcaggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttca gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgag taggagaatt gcatgttatg ttctcaatta actaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gccaaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagaca cattgtgaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaag tcgttcacgt tgtaacgcag catagcgaag cggtcgagac
     4981 caataccaaa ggcgaaaccg gtatattttt cagggtcgat attgacgttt ttcaacacgt
     5041 taggatgtac cataccgcaa ccgccgactt ccaaccattt gccgttttcg cccatgatgt
     5101 cgatttcggc agaaggctcg gtaaacggga agaaagacgg acggaaacgc acttgcaggt
     5161 catcgcgttc aaagaagcga cggataaagt cagtgaacac tgctttcaaa tcggcaaaag
     5221 tcacgccctc ttccacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac gcggtaaacg cggccggggg cgataatgcg gacgggcggc tcttttttgt
     5341 cgagcatata acggatttga atcggggaag tgtgcgtacg caaaacatca ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatattc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgt agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc tttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttca atagaggcgc gtttcagacc gttcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtca cggtacgcgt attgaccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgagcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcaattggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcggataa ggcattaggg ggttttaaaa
     6841 catcggtttc aaacacttga acccacaatg ccacggtttt ttgccgcaat taaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct tttacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttaatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacga caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caagtcttct
     7561 gctttgcggc gtaccgcaac tttgttttct tgtttctctt tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataattacc aattgaaccg gagccagcca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaatcgct ccaaagaacc caaaatagca
     7861 cgatgcaaca ttacaggacg cgcacggtcg ttgttttcgg ttacatattc tgcatccaag
     7921 cgttccggca agacgaaatc cagttgtaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctctgacat gatattcgat tttaggcccg taaaacgcac cctcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttg ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgggcctc gctgacgatt
     8221 tgatcttcgg tacagaagat atgcgcatca tcttgcacaa aaccgcgaac acgcatcaga
     8281 ccatgcaatg caccgcttgg ttcattgcgg tggcaagaac cgaattccgc caaacgcatc
     8341 ggcaaatcgc gatatgagcg caaaccgtta ttaaaaattt gcacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt gtctttatag
     8461 ttttcccagt ggccggattt ttcccagaaa gttttatcca taatctgagg cgttttcacc
     8521 tctttataac cggcagcatt caactcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggtgctt cgtcttgcaa atggaacaga
     8641 tccaattgct tgccaagttt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt agatgcgttg cagcatttca
     8761 ttgttgctgt cgccgcgcca gtatgcgccc gccagcttgg tcagtttgaa gtttttcagg
     8821 aaacgagtat tcggaacgtg cggtccgcgg cacatatcga catattcctg atgatgatac
     8881 atccccatcg cttccacttc aggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcaatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga atcttcaaca
     9241 atcgggtcgc acgcatcgac caatttgccg tttaccctgc ctgccaccgc cgccttcgcc
     9301 aaaccggcac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcgtt tctttatgca acattgcggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaaatcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaatcgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tttgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgtttaaaa gcccgattaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttctatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagttgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgttg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgaag atttgcgcga agagtcgctg aaaggcttgg aagagctcga
    10321 tttccccggc ttggccatcg gcggcttgtc cgttggcgaa cctaagcccg aaatgtaccg
    10381 catgttgcgc gccgtcggcc ctatcctgcc ggaacacaaa cctcattatt taatgggcgt
    10441 cggtacgcct gaagacttag tgtacggcgt ggcacacggc gtggatatgt tcgactgcgt
    10501 catgcctact cgcaatgcgc gtaacggctg gctgtttacc cgttttggcg atttgaaaat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagttg aacaccatcc acaatctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgaagccgtc gaacaaggca aatttgccga ctggcaagca caattccacg aaaaccgcgc
    10801 acgcggcaca gactgactcc ctgcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gcgttaccct acccgcacta tttttcagaa accgatcatg ctcccattcc ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgct
    11101 ccgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgccctt
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccaaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttat gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaatcc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgctgga
    12001 caacacatca agtgcaacct tactaccgat tcggcaagaa aatctttcac ccgctacgca
    12061 aaatcaacaa caaggtttca aacattaagg aaacaccaat gaacacaacc cgactgccga
    12121 ccgccctcgt cttgggctgc ctctgcgccg ccgcttctgc cgccgacaac agcatcatga
    12181 caaaagggca aaaagtgtac gaatccaact gcgtcgcctg ccacggcaaa aagggcgaag
    12241 gccgcggaac catgtttccg ccgctctacc gctccgactt catcatgaaa aaaccgcagg
    12301 tgctgctgca cagcatggtc aaaggcatca acggtacaat caaagtcaac ggcaaaacct
    12361 acaacggatt catgcccgcc actgccatca gcgatgcgga cattgccgcc gtcgccactt
    12421 atatcatgaa cgcctttgac aacggcggcg gaagcgttac cgaaaaagac gtaaaacagg
    12481 caaaaaacaa aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgtccccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cgtccgaacg tgctgccggc
    12781 gaaacgacaa actcgccgaa gaaatcaaaa tgctcacgga caccgatatc cgtctcggta
    12841 acgtatttcg acattttata gtgaa
//
LOCUS       DAWKQB010000049        12798 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.049, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000049 DAWKQB010000000
VERSION     DAWKQB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.049"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(951..1619)
                     /locus_tag="WJL14_001012"
     CDS             complement(951..1619)
                     /locus_tag="WJL14_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7656097.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCRLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEKEAKEKGWPV
                     LNFK"
     gene            complement(1616..2284)
                     /gene="hda"
                     /locus_tag="WJL14_001013"
     CDS             complement(1616..2284)
                     /gene="hda"
                     /locus_tag="WJL14_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ7656098.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2389..2967)
                     /locus_tag="WJL14_001014"
     CDS             complement(2389..2967)
                     /locus_tag="WJL14_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7656099.1"
                     /translation="MFRHNGEILLSGINIKLKSGDSLLIRGPSGCGKTSLLRALAGLW
                     PFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPELIEAMNTCRLGYL
                     IDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIVLLDEATAALDEPAEAALYRALKQ
                     KLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            complement(3189..3401)
                     /locus_tag="WJL14_001015"
     CDS             complement(3189..3401)
                     /locus_tag="WJL14_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656100.1"
                     /translation="MNHAETEPRDGLYVLRIGNDLFVKRVQRIPGKLLVTSENPRYAP
                     FEIDLSNTQDDIAIIGRVEWYGRSID"
     gene            complement(3564..4445)
                     /locus_tag="WJL14_001016"
     CDS             complement(3564..4445)
                     /locus_tag="WJL14_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ7656101.1"
                     /translation="MPALTVTWMIYTKYLVIFCGVFMERIDLALNLVEERSRLGYSRK
                     NFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNAVSDDLN
                     KKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGKEHITLE
                     QASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDKAETYLR
                     KWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVESLTELSD
                     EDLQKTYAAVSTKKRKK"
     gene            4493..4744
                     /locus_tag="WJL14_001017"
     CDS             4493..4744
                     /locus_tag="WJL14_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656102.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4746..6719
                     /locus_tag="WJL14_001018"
     CDS             4746..6719
                     /locus_tag="WJL14_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7656103.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPIEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVVAVLGIKYEYDCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFASAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     EELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFEADR"
     gene            complement(6786..6863)
                     /locus_tag="WJL14_001019"
                     /pseudo
     CDS             complement(6786..6863)
                     /locus_tag="WJL14_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6934..7848
                     /locus_tag="WJL14_001020"
     CDS             6934..7848
                     /locus_tag="WJL14_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7656104.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIDTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIVEVMRAAHEGGETV
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLAVAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIARAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            7938..8204
                     /locus_tag="WJL14_001021"
     CDS             7938..8204
                     /locus_tag="WJL14_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656105.1"
                     /translation="MQVLKKVDWKMFVARSFWRCVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEKMERQADLEVKVWEAQYESMSVEEKMKGIVYE"
     gene            8313..8468
                     /locus_tag="WJL14_001022"
     CDS             8313..8468
                     /locus_tag="WJL14_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656106.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQARFRRPA
                     EKKEEKK"
     gene            8667..8834
                     /locus_tag="WJL14_001023"
     CDS             8667..8834
                     /locus_tag="WJL14_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656107.1"
                     /translation="MNAKEIAEWIEDRGELMIMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8944..9462
                     /locus_tag="WJL14_001024"
     CDS             8944..9462
                     /locus_tag="WJL14_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ7656108.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGHVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(9622..9837)
                     /locus_tag="WJL14_001025"
     CDS             complement(9622..9837)
                     /locus_tag="WJL14_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656109.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLDMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(9841..10449)
                     /locus_tag="WJL14_001026"
     CDS             complement(9841..10449)
                     /locus_tag="WJL14_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HEZ7656110.1"
                     /translation="MKDNFTMNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSK
                     WIQRRIEEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERND
                     KGRQARRYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIG
                     KLLDMNPGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            complement(10581..10781)
                     /locus_tag="WJL14_001027"
     CDS             complement(10581..10781)
                     /locus_tag="WJL14_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656111.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            complement(10982..11167)
                     /locus_tag="WJL14_001028"
     CDS             complement(10982..11167)
                     /locus_tag="WJL14_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656112.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(11440..11607)
                     /locus_tag="WJL14_001029"
     CDS             complement(11440..11607)
                     /locus_tag="WJL14_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656113.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            12130..12321
                     /locus_tag="WJL14_001030"
     CDS             12130..12321
                     /locus_tag="WJL14_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656114.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            12318..12734
                     /locus_tag="WJL14_001031"
     CDS             12318..12734
                     /locus_tag="WJL14_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656115.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
BASE COUNT         3391 a         2577 c         3385 g         3445 t
ORIGIN      
        1 tgatttttgt taatccacta tacgtcccag attcccactt tcgtgggaat gacgggatgt
       61 gggtttttgt gcggatttga accggtaagg gtggtgtggg attggtggtt tgcttaggat
      121 cttttggatt gtagtttgta tatacattta cttgttgata aaagataaaa taaaattaga
      181 aactaaaagt gagaaaaaat taataataat agggatgtat aaatgtaaag gctccgtttc
      241 atagctaagg ttatctgaat atatggaaaa aaagtaaaag tccataaaac taaaatatat
      301 agataatgct aatgataata gaattatcac tttcctagag taaggataat atttttttat
      361 cgtaaaaaaa tataaaatag gataaataat tgccatatag atagattttg taacaaaatt
      421 aaaaataaat aataaaaata atgtaataaa gtatattttt tggctatgaa ataaaattgt
      481 acataattga acgagcagat caaaaaatga actacatata acaataaata ataacgtatt
      541 taccatacta aatttaatag gtctcattat catatttaat aaccacttca tagtaaatgc
      601 agagtgggtg gaaacactca ctttatggtt tgctacgctc tgctcaatta gcaacccgat
      661 aacccaatat ggataatagg gtaattaatc caatctaatt tgtcagcatc cgttaattta
      721 ttgcaaaata aagtattgaa ttatgtcggg tgcaaatgac gaaatataag tttccgtgcg
      781 gacggatcaa gattcccact ttcgtgggaa tgacggtgga aagattgttg tttttcccga
      841 tgaattcctg tgttttttgt ttttccggat aaattcctgt ggctttgagt tttttggatt
      901 tcagcctcaa tgccgtctga acgccgaatc gggcttcaga cggcattgcg tcatttgaaa
      961 ttcaaaaccg gccagccttt ttctttggct tctttttcca gctcggcatc ggggttgacg
     1021 gcgacgggtt cgtcgacgag gcgcagcagc ggcaggtcgt ttttggagtc gctgtaaaaa
     1081 taggttttgc cgtagctttc gagcgtttcg ccgcgttcgg cgagccattg gttcaggcgg
     1141 gtgattttac cttctttgag gctgggcgtg ccgatgtaat tgccggtgta gcggccgtcg
     1201 gaaccggatt cgagttgtgt gccgatgatg ttggtaatgc cgaaaaggcg gcagacgggg
     1261 gtgatgatga actcgttggt cgatgagatg acgagggttt cgtcgcctgc catttggtgg
     1321 ctctgcacca gcatacgctg cataggcgag atgtggggga tgatgtattc cgccataaat
     1381 tcgcggtgaa attccgccag ctcttctttg ctgtaacggg cgagcggggc gaggtggaat
     1441 ttgaggaatg cgtcgatgtc gaggcagccg ttttggtagt cgcggtagaa tttttcgttt
     1501 tgcgcttcgg tttcggcagc gtcaaccaag ccttttttga tgaggtattg cggccaggcg
     1561 tggtcggaat cggtgttgat gagggtgttg tcgaggtcga agatggcgag gtttttcatt
     1621 gggtttcctg ttgtttcaaa agctggcgca aaagcggcag ggtgatgcgt ttgcccatcg
     1681 tgacggcgta gttgtccagc gtgtcgagca tcatcatcag gctgtccata tcgcgccgcc
     1741 agtgtttgag caggtattcg aaaatttcgg aatcgacggt tacttggcgt gccgccgcca
     1801 tactggcgag cgcgtcgatt ttttcttggt cggttaaggg tttgacttcg taaacgaggc
     1861 agtacgccat acgcgtccgc aaatcttcgc ggatgacaag ctgctggggc gtgtattccg
     1921 aaccgagcag caaaaagcct ttgccgctgt tgcggaagcg gttgaagatg gaaaaaagca
     1981 gggcttgttc ttcgttgccc agtttttcga cttgatcgac ggcgaggtat tccgcctcga
     2041 acgcggcatc ggtcagcggc atggaggcgg catcgatata ggcggcgttt ttgccggctt
     2101 cgagcgcctg tgcgacccac gcctgcaaaa gatggctttt gcccgcacct tcttcacccc
     2161 agacatagat aaactgtccg tgtttgtgtc ggaggacata gaccagttcc gcgttttccg
     2221 tgccgaggaa tttgtcgaaa ctcggatagt cgtgtgcggc aaagtcgaaa ataagctggt
     2281 tcacggttcg gcattccgag gggtggtaaa cgggtttatt gtacgttgtt ttcgcgtgct
     2341 ttatcaattt gaacgatgcc gtctgaaaac ggcttcagac ggcatcgttc aaccgcaggc
     2401 aacgttgccg acatcgaggc gcatattgtg gaacgcgttg agcgtgcttc ggtgtccgat
     2461 gctgatgatg atgctgtcgg gcagtttttg ctttaaggcg cggtagagcg cggcttcggc
     2521 cggttcgtcc aaggcggcgg tggcttcgtc gagcaggacg attttgggct tggaaagcag
     2581 ggcgcggacg aaggcgacgc gttgcagttc gcccagggag agtttgtgct gccagtcgtc
     2641 tgttttgtcc aatttgtcaa tcagataacc caagcggcag gtgttcatgg cttcgattag
     2701 ctccggatgc tgtttgtcga tgtcgggata acaaaccgcg tcgcgcaggc tgccctgtgc
     2761 cgtgtacggg cgttgcggca ggaagaggat gtcttgatgc ggcggacggc tgactttgcc
     2821 gctgctgccg aacggccaaa gcccagccag cgcgcgcagc aacgaagttt tgccgcaacc
     2881 gctcgggccg cgtatcagca gggaatcgcc gcttttgagt ttgatgttga tgccgctcaa
     2941 caggatttcg ccgttgtggc ggaacagagc gacgttttcc tgtgggggac tgttagtttt
     3001 tgcacaagga acaaatagag taaaaaaacg ctgaaatctt cggaagacgt ggatttcggc
     3061 gtttttttgt atccggaaaa gttacgccag ctttttcaca aaaccgcgcc ggaatgcgcg
     3121 gttttctgtt taaagctgac gagattaggg aatttttaaa actgttttaa gaggttttta
     3181 aaatggattt aatcaatact ccggccatac cactcaacac ggcctatgat ggcgatgtcg
     3241 tcttgggtat tactcaaatc tatttcaaac ggtgcgtaac gtggattttc agacgttaca
     3301 agcagtttgc ccggtatacg ttgcacacgt ttgacaaaga ggtcattgcc tatacgcaag
     3361 acatataggc cgtcgcgcgg ctcggtttcg gcgtggttga ttagaatgtt tataaaaggc
     3421 tttaactaga aactgaacat ggaacttgta taatagttcc atgtttgaaa aaatctttta
     3481 atatcataca tctaaaaaaa gcatctgaag accataagct aaacatgaaa cttgaattta
     3541 aaggcggagt ttcatgttta agcctatttt ttccgttttt tagtcgatac tgctgcatat
     3601 gttttttgca aatcttcatc acttaattca gtcaggcttt ctactgagaa atttttttcg
     3661 aggtaacttt tcaaccaatc ttcaagctca agctgtttta catttaattt gatataagcg
     3721 tatttctttt ttcgccagtc gggatcgttg ttctttgatg ttgctgtatt gctcaaccta
     3781 cccagccatt ttctgagata agtttctgct ttgtcgtaat cgctcaatgc gattagtttg
     3841 taacttggaa ctttacagtg agcattcaga ctagcccaaa ttgcacggat tgattttggc
     3901 gaccgtttgg caatctcttc tgctgcggca acttgtttaa caagttgttg cagctttgaa
     3961 gcttgctcaa gcgttatatg ttccttgccg ggttttgaat cgacacgggt tctgatgacg
     4021 tgattttggg tattaatttg atgaacaacc gaaccagatg aggcgataac agcgttactg
     4081 acgttgccgt taatctgatt ctcaacgttt gatttgccaa tagcgtcgtc caatttttta
     4141 ttaaggtcgt ctgaaacagc atttaaattt gccgaatgaa tgcctgtaaa gacaaattga
     4201 atatccaccc ctaattggac agcagcaagt aaaaagtccg caggaatgtt gacctgcccg
     4261 ctttcataca gtctcaaact ttctgcactg catccagttt gctcagcaaa attctttcga
     4321 gagtatccaa gtcggcttct ctcttcaact aaattcaatg ctaaatcaat gcgttccata
     4381 aaaacaccgc aaaatattac caaatatttt gtgtatatca tccaagtaac agttaaggca
     4441 ggcattaaag cctgtaaggt aagtaagtaa tggattctat catggaggca ttatgcaagc
     4501 gaacgaaatc tataaatcct tagaagaaaa aaatatatcg gcaagaatgt tggcttcggc
     4561 tttaggggta actaaccaat ctgtttctga ggtcataaga aatggacgag gaagcaaaag
     4621 aattgcggaa gcaatcgcga aagtgattca aaaagattta gttgaagttt ttccgcatta
     4681 caagaaaaca gattgtcgag acaaaaagat agctgaattg aaagagatgc tcggagtggg
     4741 ttgaaatgga atatatggat ctcaaaagcc tgcttaattt tgggttgcca aatatgccaa
     4801 gaacagtaat agggctgaga aaaaaagcgc aaagggatgg ttggcaaacg agaaggcgtc
     4861 aaggtaaggg tggtggtgtt gaatatgccc tacctattga aatccgagca gccatcatga
     4921 aacggcagtc ggacgagctg gcggagaaga tgccgaaaac cctgccccaa gtcagaccag
     4981 ggacggcgat gtcggctcag gcactggctg aagcggccaa gctgttgaac gagaaacaac
     5041 ggtcggtggc ggatgcgcga tgtgcggtgg tggtggcggt gttggggatt aaatacgaat
     5101 acgattgctc tgccaaggct gcggtggctc agtttttggg cttgctggca gaaagtaaat
     5161 tggacgcggt cacgcttggg aacttggaaa aggccaatga ccgcagccgg tcggcaaagg
     5221 ttggcgaacg tactttagac ggctggattt ctgcttattt gaaagcggaa aacgcgacgg
     5281 agcggctggt tgctttggct ccgaagacga cgaaggcggt taagccgatt gagagctacg
     5341 gttggttgcc gatgtttatg cagtttcaca acatcccttc agctccgaag ctggcacaca
     5401 gctaccgccg gtttgtgcag tgggcggaag cggaaaatat gccggtcaat gatgtgccta
     5461 atttgagtat ggtgcggcgc gtttgggaca agctgccgct gattatgcag gagcgcggca
     5521 ggaaaacggg ggcggcttat aaatcgctgc tgccttatgt gaaacgtgat tggggggctt
     5581 tgaagccgaa cgatgtttgg atcggcgacg gccacagctt taaggcgaag gtggcgcatc
     5641 cggtacacgg caggccgttt aagccggaag tgacggtgat tattgatggt tgtacgcggt
     5701 ttgtggtcgg tttttcggtt tcgttggctg aaagttgtgt ggcggtatcg gatgctctgc
     5761 gtatcggggt caagcatttt ggtttgccga ttatctatta ctcggataac ggcggcggcc
     5821 aaaccggcaa gacgattgac catgaaatca cgggtattac gtcccgactg ggtatccgcc
     5881 atgaaacggg tattgcgggc aacccgcaag gtcgaggcat catcgaacga tggtggaaag
     5941 acaatctgat tgagatggcg cgacagtatg agacgtttgc gagcgcgggg atggacagca
     6001 gcacgaagaa cctgatgtac cgcaagatgg aaagtgcttt taatgctttg gaaaaaggca
     6061 aggatttgac ggaggaacaa cagaaatatt tgaaaaaact gccgagctgg tcgcgcttta
     6121 tcgcggatgt ggtcaagtgt atcgatgaat acaacaaccg cccgcacggc gagctgcccc
     6181 gacatcctga cggcgggcat tattcgccga aggcttatcg ggaaatgagg ctggaacagg
     6241 acggtatcgc gccggatatg ttgtcggcag aagagctggc gacgatgttt atgccgcaag
     6301 aggtgcgaaa ggtacagcgc ggttggctgg atttgttcaa caactcttat ttctcaactg
     6361 agctggcgga gtatcacaaa gacgaggtac gggtcagcta cgatttgagc gatgcgtcgg
     6421 cggtcaatgt gtttgatatg gacggcaagt ttatcactaa ggcgcaggcc gacggcaata
     6481 cccgcgaggc tttcccgacg gctcgtatcg accaactggc ggaaaaacgc cgaaaaggca
     6541 aaatcaagcg ggcggaaaat gcaatcaagc tcgcaaatgc ggaagtcaat ccggctttgg
     6601 aacaggcggc agtttgggac gagctgggac atttgggcgg aaacgtcatc gaggcggagt
     6661 atgcggtatt gccgaaaacg ggcacggacg attttgtgct atttgaggcg gatagataaa
     6721 ggaaaacatg atggacaaac agaaaaatgc ggcgttttcg accgagcttg ttgaaaaatt
     6781 gaaactcaag cgagcccttg ggcggattca acgatttcaa gtaaagattc aaggtgttcc
     6841 cgctgaacgg aatcaggctc aaatttctaa atcgggaaac tctttcattt cagacggcct
     6901 ttaaaggttg tttaaaactc aaggatatta aaaatgaaac aaatcaatca agcattgcaa
     6961 caaaaactgg ctgagtttaa agaaaaatcg ggtatgagcc aaacccaact ggcacgcggt
     7021 atcgatactt cgccggcatc cgtcagtatg tatctgaacg gtacttatgc ggaaaaaggc
     7081 ggtaattatg aaaccatcga gccgaaaatc gaggcgtttt tggagatgca ggacagtaaa
     7141 gcgaaacgcg aagagctggt gttgggtttt gtatcgacta agacgacccg ccgaatcgtg
     7201 gaagtgatgc gcgctgcgca cgaaggcggc gaaacagtgg tgatctacgg tcaggcggga
     7261 ttgggcaaga ctcaggcggt caaaaactac tgcgagaaaa accctgcggc catcttgatt
     7321 gaggctaatc cgagctttac ggcacttgtg ctgatgcgca agttggcagt agcggcgaag
     7381 gtatcggcga tgggcagttt gaatgatttg tttgaatcgg tatcggacag attgcgtgat
     7441 tcaggccgtc tgattgtggt cgatgaagcg gaaaacctgc cgttacgcgc ccttgagatt
     7501 atccgccgac tgcatgatga aacgggctgc ggtttggtgt tgagcggtat gccccgactg
     7561 gtggccaacc tgcgcggtaa gcatggcgag ctggtacagc tttacagccg cgtgtctgtt
     7621 gcgctgaatt tgggcgaatc tttgccggat gacgaactct ttgagattgc gagagcagct
     7681 ttgcctgatg cggacgaaga aacgctcttg gaactggtta aacatagcaa cggcaatacg
     7741 cgccggatga gcaaattgat gcgcggcgcg gtacgcacgg cgaacaagaa cggtatcaag
     7801 atgcaggccg gtatcgttaa gaaatacagc tccctgatta tccgataggc cgtctgaaac
     7861 ggtaagtctc tgacagggct atatattttt taccctatga ttttaataag ttattgtttt
     7921 ctaaaggaaa acgcaaaatg caagttttga agaaagttga ttggaagatg tttgtggcac
     7981 gctctttttg gcggtgtgtg ccggttggtt tgacggtggg cgtgtggtgt tttgtggcgg
     8041 gaatggcgtt gcattcctgc gcgaaagaac ccgaaccggt tgcgaaagag ccgacgaagg
     8101 ttgagaagat ggaaagacag gcggatttgg aggtcaaggt atgggaagcg caatacgagt
     8161 caatgagcgt cgaggaaaaa atgaaaggga ttgtttatga gtgataagcc aattttattg
     8221 agccctgcgg cgaaaaaaga ggctttggat cgggcggtca aggaaatccg tgcgaaatat
     8281 ggcgataagg cgattgtgaa aggatgtgtg aaatgagttt cggacgacgt aatacggatt
     8341 ggcaggcttg gggacaacac cgcaggcgtg cgacggcgcg aatggcgcaa aaaagccggg
     8401 agcgcgaaat cgaagaatat caggcgcgtt tcagacggcc tgccgagaag aaggaggaga
     8461 aaaaatgatt tggtctgttg tcggattggc ggtgttggtg ctgctgggga tttggcttga
     8521 aatgccggcc agaatcgtcg tgttgcacat gataggcgaa ggccatgacg ggtatgacga
     8581 caattaaaac ggtaagccgt tgatgttgct ctatattttt ttgtcttatt gaaaatataa
     8641 ggtattgatt taaaaggatt taggaaatga atgcaaaaga aattgcagaa tggatcgaag
     8701 accgtggcga gctgatgatt atgaaaaagg acggcgaagg ctttgtgatt gcagcgcgtg
     8761 cgccggacgg gatgtggaag actgccgagg cggaaacttt ggctcaggcg ataactttat
     8821 gggaggaagt gtgatgttta agtttttgag aaaccgcgcg aaagttgaag cacttgccaa
     8881 gcacgaacgc gaacaaggct tgagtaagcc gaaatgttaa acccaacctg aaaggaagag
     8941 aaaatggcta aaacccgaat caaacagccc gccatcgaag cggcacaaga caaaacggaa
     9001 gttactgcat ttatccgcca aatcggcgac ttgcagcgcg aagtcaaacg cctggaaacc
     9061 gaagccggag acaaaaaagc ggccatcgaa gaagaatatg ccgcccaagc cgcgccgatg
     9121 tgtgccgaaa tcatgagcct gaccgaacgt gtggccgcat actgcgaggc acataaggac
     9181 gagctgacgg aaaacggtaa aaccaaaacc gtggacttta ctaccggcct gattaaatgg
     9241 cgcatccgtc cgccatccgt caaggtaacg ggcgtggccg ccgtcttggc gtggctctcg
     9301 gaaaaatccg cctttgccga gtttgtccgc actaaaaagg aaatcgacaa agacgccatc
     9361 ctgaatcaaa aagagcgttt ttcagacggc cacgtgccgg ggattaagat tgtgagcggc
     9421 gtggaggatt ttgtgattga gccgagcgaa caaattctat aaaatcaaca gcctgatttc
     9481 aattcagtgt gttgaaacgt gattgagcct acggagcagg agcttgccta gccggttcaa
     9541 ataggcttta acccatgatt aaaggccgtc tgaaatgggg tttaaaacct gtttcagacg
     9601 gccttttttc ataccctaga ttcaggctgc tttctcttcc tgctcgtaca cggcgttgta
     9661 gaaagcggcg gacagcttgt cggctttctc ggaggcggtt tcaatcacgg cagacaaacc
     9721 gtcttcgatt ccttccatgt ccatatccaa aactttcaga tggttgagcg tgaacaccag
     9781 cagattcagg gctttgaggc tgtcttggtc gaaagtcaga gtataggtgg tattcatggc
     9841 ttaaccctcc aatcccaaag actgttgttg caccaccatt ttgggcttgg ggacatattc
     9901 caagaaaccc aaatcgttga gttttttgag acggtaggaa accgcgccgg ggttcatgtc
     9961 caagagtttg cctatttcgg tcaggttcaa gcccatactg cggtagcgca gcagcgcgag
    10021 catttcgggc gcggcttgga aataggcgtc ttccaatgca tcgatgcggt atagcacggc
    10081 atcaggcagg gcttttgcct gtttctccat ttcgataaaa tatcggcggg cttggcgtcc
    10141 tttgtcgttg cgctccacca tgcacagctc ttttgccatg tcgagggata aataataccc
    10201 ctgaacagtt ttatcacgct gtccaaagaa acctgcttcg gtgcggacaa ttttgtccac
    10261 gccgataaaa tcaagggctt gtgtaaatcc gtattcttca atgcgacgct gaatccattt
    10321 agaaaatggt gtttccacac ctaagaactt atgcaggtca tgagcatcaa ccagtgcttg
    10381 tgtttgattg tccaattgac ctgaaacagt tgggatgagt tgggtattca tggtaaaatt
    10441 atctttcatt tcaaggttga gatgacaagg aaaagaacgc tctaacgttg cttttccact
    10501 atccccgata agctgtcact tatcggggat ttccatatct tgtgttgcac aagatatgga
    10561 aattttaaat tatggtaatt ctatttgtca atattctttt ttgctttctt tttgaatttt
    10621 tccgattcca tatctcctct aattttcgat tttacccagt ttgaaaaatc taaattttca
    10681 gaaaatttaa ttaaatcaag ttctttttcg gaattgaacg atactgtttt cactactcgc
    10741 ttgagtgcgt atttttcttg agccttttta atcgagtcca taatcttcct ttaaataggt
    10801 tttaaaacac gattatggta atactaaaaa ccattttaat caagatgtat tacaacaaaa
    10861 agccgtctga aatggggttt aaaacctgtt tcagacggcc ttttttcatg ccctacataa
    10921 agagagttgc tgctctgctt tccacctgtt ccgccgatgt tttcccatcg gcggaatttt
    10981 cttatttcat tttttccttc atgactgatt taacccagtt tgaaaagtcg ggaatttctt
    11041 tttgcataaa atccaccaac tctttctctc tttccaaatg gaaagaaact gttttcatca
    11101 gccgcttttg gtcatacttt cgctgatatt cagcttgttt tgcttttagc gttttttcat
    11161 caggcatagg caatccttga tttttaaaat cgttgtagta aaatggcttg cagaaaggtt
    11221 ggcgaaaggg gcgcggtgtt ctgccacccc ttgtgtttaa tcaacttgct tagtaagccg
    11281 gactacagct taaaagcaga atgatgaaaa aaacaacttt cgccagtttt gtcatttcca
    11341 caagcctcct ttctatccga taagcccccg aagttgccgc ttcggggctt tgctttatca
    11401 gataggcgta ttatctagta tttctagaat ttagtcaagc tattttttga ttttttcttt
    11461 gatgattgat ttaacccatt gggaaaaatc gatattttgg gcatattcca aaagttcttt
    11521 ttccgtttcg gcattgaacg agactttctt aatcacccgt ttctgctcgt aacgctttcg
    11581 gttttctgcc agtttttcat caaccatagg caaactcctt gattttttaa accgtatttt
    11641 gtaagattgg gactggggac agcggcgacc gcccccagct ggtcagtctt tagtaggcat
    11701 tagcacttgc caacatgaaa actgcgacca agatgagaat ttgaatagtg cgtttattca
    11761 tcttctttct cccagttgag ccccgcttcg gtggggcttt tcccgtatcg gccctcacta
    11821 cgccgatgaa ttgaattata aacgtggaca cgttaaaagt caaccctttc cccaacaaaa
    11881 tcaaagaaaa atgccgtctg aaaacagctc gaatgctgtt tcagacggca ttttttgttg
    11941 tcgggcatca atgccctgcc atttgtgcgg cgttgcggtt atcaagatat ggtattttag
    12001 tgtgtatttt tagaagaaat ttaccatata tagtattaaa atattatacg gttgttttat
    12061 atggatttga ttttcttgaa aaataaatcc catatgctaa tatttgcact atttgggcga
    12121 ggttgaaaaa tgaataccgt caaagaatat acggcagtcc gcgaacggct gctcaatgct
    12181 gccgattatt tggaagaagt ccgtaaagac cgtaagacgg gcaatattgc gtccgtggaa
    12241 tttgtgccgc cgaaaatagg ggctcggggt tacggcaaat tcaaagtccg ttacaaaacc
    12301 ttagtggcgg ttgatttatg agcggggaaa aatccgagaa tctgccggcc gatgagcagc
    12361 agaaaatgct tcaggagttt ttagagcttc aaaaacagga tattgcagtc aaacagaagg
    12421 agttggaaag ccgtaaggac gaaatccaat ctcacgaacg cattgcactg gctaccatcg
    12481 aagcgcaaaa acagggtgaa cagcaacatg gtgagatttt caaagaggta cacaaacgcc
    12541 gtctgaatgc cgtgattgct atttcttttc tgattgccgc cgtgctgatt actgcgttat
    12601 ggcgcgataa ggacagcgtc gctattgaaa ttgtcaaaat cggcggggcg gtagctttgg
    12661 gatattttgc aggaatcaac agaggtaaag cgcaaacctt ggaaaagcaa cggcgcgaaa
    12721 aagacagtga ttagattgta gtaaaaaagg tcgtctgaaa cgttttggcg aaactcgcaa
    12781 agctcgtttt cagacggc
//
LOCUS       DAWKQB010000050        12657 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.050, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000050 DAWKQB010000000
VERSION     DAWKQB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.050"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..103
                     /locus_tag="WJL14_001032"
     CDS             <1..103
                     /locus_tag="WJL14_001032"
                     /inference="COORDINATES: protein motif:HMM:NF016757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PT domain-containing protein"
                     /protein_id="HEZ7656116.1"
                     /translation="SLQPSLQPSLQPSLQPSLQPSLQPSLQPISTAR"
     gene            complement(100..2073)
                     /locus_tag="WJL14_001033"
     CDS             complement(100..2073)
                     /locus_tag="WJL14_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ7656117.1"
                     /translation="MHPIETPDKTFHDGDGVSEFGTILPAWWLNQVQSELLTVLNAAG
                     IRPDKSQPNQLLAALNRLAVVITGDQTITGQKTFTAATQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHQANKPRFNEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGVYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQVSSANDNWSAWKKLNSDGIPVGAIVSFPKAVQNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTKTAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSSLVSTWTDENLSDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRDHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(2084..2644)
                     /locus_tag="WJL14_001034"
     CDS             complement(2084..2644)
                     /locus_tag="WJL14_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ7656118.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2641..3158)
                     /locus_tag="WJL14_001035"
                     /pseudo
     CDS             complement(2641..3158)
                     /locus_tag="WJL14_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236967.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(3109..3342)
                     /locus_tag="WJL14_001036"
     CDS             complement(3109..3342)
                     /locus_tag="WJL14_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HEZ7656119.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPTARRRQPLRL"
     gene            complement(3339..4484)
                     /locus_tag="WJL14_001037"
     CDS             complement(3339..4484)
                     /locus_tag="WJL14_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7656120.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GLTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(4474..5805)
                     /locus_tag="WJL14_001038"
     CDS             complement(4474..5805)
                     /locus_tag="WJL14_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HEZ7656121.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAAAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAVINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDIARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(5805..7976)
                     /locus_tag="WJL14_001039"
     CDS             complement(5805..7976)
                     /locus_tag="WJL14_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HEZ7656122.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            8131..8733
                     /locus_tag="WJL14_001040"
     CDS             8131..8733
                     /locus_tag="WJL14_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656123.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLFTALTEVV
                     KESNQTGATAQKDVPADGILYSVVFEKETNTIAIIGRKQP"
     gene            complement(8777..9010)
                     /locus_tag="WJL14_001041"
     CDS             complement(8777..9010)
                     /locus_tag="WJL14_001041"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656124.1"
                     /translation="MRRRRRSSKTQLPDGTQKLPSGGHPDGKAGIGADAVRDMCHAEL
                     SAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(8985..9350)
                     /locus_tag="WJL14_001042"
     CDS             complement(8985..9350)
                     /locus_tag="WJL14_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656125.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     RLKPSAATASPDAPSAAEQ"
     gene            complement(9354..9731)
                     /locus_tag="WJL14_001043"
     CDS             complement(9354..9731)
                     /locus_tag="WJL14_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ7656126.1"
                     /translation="MSDATYADAVIMEMNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEDRRTSYLDCFTVDTG
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(9796..11205)
                     /locus_tag="WJL14_001044"
     CDS             complement(9796..11205)
                     /locus_tag="WJL14_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEZ7656127.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVADRLKTAITAADVTVTAS
                     GSGAAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANAFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVSNAIEQRGCIGVLGMSAALSTATTATGEINDGRM
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPLFAECNNA
                     LYNGLTPLTVVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(11208..11405)
                     /locus_tag="WJL14_001045"
     CDS             complement(11208..11405)
                     /locus_tag="WJL14_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HEZ7656128.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(11402..12052)
                     /locus_tag="WJL14_001046"
     CDS             complement(11402..12052)
                     /locus_tag="WJL14_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HEZ7656129.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(12042..12266)
                     /locus_tag="WJL14_001047"
                     /pseudo
     CDS             complement(12042..12266)
                     /locus_tag="WJL14_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
     gene            complement(12256..12636)
                     /locus_tag="WJL14_001048"
                     /pseudo
     CDS             complement(12256..12636)
                     /locus_tag="WJL14_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2478 a         3636 c         3414 g         3129 t
ORIGIN      
        1 atctctacag ccatctctac agccatctct acagccatct ctacagccat ctctacagcc
       61 atctctacag ccatctctac aacccatctc tacagcccgc tagtcttgca gtgcctcgag
      121 caagccgtct cggcggcggc ggtttcgggg tccggttgat gttcttccac tctctctttg
      181 cggttcttcg ttggtttcag gtatttcggg gcctatcggg gagctggcgg cgtttcccga
      241 ggcggctttg ccgatggcca tccagtcgca actgccttcg taccagttgg taataatggc
      301 ggcggcgtgg ttttttgcgc ggataaacag ccagttgtgt tgccttcgat cgccggtaac
      361 gcgtcccgaa tggcgttcgg atacgaagca tttgacgttg ccgtcggcaa aggcgacggg
      421 gaagacgact tcgttttcga tggggccgcc gcttcttgta aacctgtaac tgccggtctg
      481 tatcattgtg ccgtcgggga atctgacaat atccacgccg ccgatggttt ggcgtgtgaa
      541 ttgcgcggca acggcggtgc tgattttttc gtccagccct tggatttggg cggcggtgtg
      601 ggtgtggtcg cggtcggctt tgtcttggag gttttgcgcc agcgtgcccg cgtccagcgc
      661 gcctgcgttg atggtttcgc cgtgggactt tatccagaac acggcttcgc ccaaggtgtc
      721 ggcggctttg atgcacagtt ttaaaaccag ggctttgggg cggttttcgt cgccgcccgt
      781 tgccattttg ctgtccgggc gcggggttaa aaagccgttg tcgcttaaat tttcgtccgt
      841 ccaagtcgat acgagcgagc ttctctgcct ttcgttgtgg tcttcgtaac cgagggctgc
      901 cgcgtgcggg tggtttaccc agtgtgaaaa tactttgtgg acgtgccgtt tgatttcgtc
      961 ttcctgcttc gttccgactg ccaagctgtt gcctgcgttg cggataaagc ggtcttccgc
     1021 ctgcgggacg ttttggatgc tgccgtattt tccggtcagc aggcggtaca gctcgggata
     1081 agcggttttg gttacgcgcg tgcggatgtc gtcaaacgcc agccagccgg tcgggatttg
     1141 gtcggacgga aaccacgcgg tgatgccgat gtcggtacgg cttaaatcgg gcaggcggtt
     1201 gctgttgccc agggcgcggt agaggtcggg gaaggtgttt tgtgcaaagg tcgtgccgtt
     1261 ggctttgaga tagcctgcgg ggttttgtac ggctttggga aaggatacga tcgcgccgac
     1321 ggggatgccg tccgaattga gttttttcca tgcggaccag ttgtcgtttg cgctgcttac
     1381 ctggtggcgt tcgtacacgt cggacgtgta ggcgggataa cccaactgcc tgcaccagcc
     1441 gggttgcgtt ccggcgatga cttggatatg gcaggcggtg ttttcaacgg gcaggttgga
     1501 gctgccgacc gccgtcggca gggaatagac gccgtctgtt ttgagggtgt tcaaatcgcc
     1561 ccggaaggtt tctactttga agttgccgat gccgtatccg gacagtgtgt tcggtttgcc
     1621 ttcgatgtct tcgttaaaac gcggtttgtt ggcttggtgg aagatgtcgt ggttgttgta
     1681 ggaaatgcgg ttgtttgcgc cgtgcaggcg cagggtgtcg tcgccgaaga cgatgtgggc
     1741 gttgtcggca tccgcgccga tgtaggcttt gtggccgccg ttccagttcg tctggttggc
     1801 ggataaatgg atgccgcttt ggaattgggt tgcggcggta aaggtttttt ggccggtgat
     1861 ggtttggtcg ccggtgataa ctacagccag cctatttagt gctgccagta attgattagg
     1921 ctgcgactta tccggccgga taccggccgc attcagcacg gtcagcagct cggattgcac
     1981 ttggttgagc caccacgcgg gcaggatggt gccaaattcg gacacgccgt cgccgtcgtg
     2041 gaaggtcttg tcgggggttt cgatggggtg catggtttag tccttatcgg taggtaaatc
     2101 ggatagcggt gtgggcgggc ttgaggcggt tgaacaggct ctcgatcacg gcatcgctgt
     2161 aatcggtcag cctgtcgccc gccgccgaga taccggcgcg gaatcgggta atgcggttgt
     2221 tgccgccgcg cacgttaacg tgccacaccc acatgatttc ctgcggcgca agacggtcgc
     2281 cggcgcggtt tacaccggcg cggaacggct gcggttcgtc gatttggatt tgatagcccg
     2341 ccgcctcggc caaacgcaca aaataaggaa tactcaagcc gcctgtttcg tttagcttgg
     2401 ccatgacggc caacacacgg cgctggcggt ttttgcccgt accgtccaaa cctaatacgc
     2461 gctcccaatc ggccagcatt tggccggcgc tgcgtgggtc gacggcatcg gcaacgcttt
     2521 gagccgattc cgccaccgca tccagcgctg cgccgtctat ttctgcctgc gctcgcacac
     2581 gcggggcatt gcgggcatac gacacggggg gcaacaggcc ccgcaagatg tcttgatagc
     2641 tcatgacggc atctccgtta cattaatcgc acccgcttta aaccactcga tgcggttaac
     2701 cgtatcggcg gcacggttgg ccgtcggcgc agtcagacgg cggtcgatca caccatccac
     2761 attgctgata gcagcctcga tttgcgacac agtcaggccg tcgccgggat cagggtgtcg
     2821 aaatattctt ttagggccgt ccgtatgcgg cgcttggcct cgtccaagtc gataccgtcg
     2881 agcttgactt gcacggtaac aggcaccgcc gttacggttg gcttgagtac cagcgcattt
     2941 tttgccgtta ccgggcgcat ctcgtcgata taagcctgta cgcggcgcac agtttcttcc
     3001 gacggcacac cgtcggcgga ggtaatggca atatccaccg tacccaagcc gcggcgcagc
     3061 ggataaacat atgcgctggt tacgccgtca acactcaacg cccagttttt atagtcgtaa
     3121 cggttgccgc ctgcgggcgg tcggcggatg gctggcaaag ccgtattcct gcaaatgttc
     3181 aaggtcttgc agggtttcgt cggccaagcc gctcaactgc acgcgctgta tcggctcgga
     3241 cgacaccacc agcgtgattt ttccccgaaa ggccgcgcgc agggtttcgc cgatattttt
     3301 agcagtttgt gccgtttttt tcgccaattt actcaaactc attcaaacac cgccgtttct
     3361 gcttgttttt tgccgccttt tttgccctta tggctcacgc ctttgcgttt gcctttgcgc
     3421 ttgcgcgccg cctcggcctt tttggggtaa gcgtcgggtg tccaaatacc gtcctctttg
     3481 agccgcagct cggtttgcgt gccatccatg cgggatagca taaaccgccg ccccatcaga
     3541 aaaaacaccg catcgatacc gtgctcgtcg tcgatcacat gcacacgctg gccaggttgc
     3601 cacaatacgc cgtcgcgggt tttatggccg cccacggtta tcgtgagtgt caatccctcc
     3661 agccgccagt cggccagctg ctttttagcc tgcttttgca atgcggccaa attgtcggca
     3721 tcggacacca ccaccgtttt agggcggtgc agcgtcatcg tcgggtcttt gtacacccac
     3781 tttaaatcgt gtttggcgct gtcgccgctg cggccgtgcg attgcgccaa aaaagtaacc
     3841 tcggaaaagc ggttgtcggt atcccactca atgtccatgc gctcgatatt gcggcggctg
     3901 tcggtgcggc tccaacacaa tgtcgccacc ggcgggctac tgtaatccgc accgcccacc
     3961 accaacgtgc cgtccggctc cagccacgga tgcagcccga ccgagttggc aatatgggtt
     4021 aatgcctgcc atacggtttc gcccggctcg atgtcgattt tgtccaaagc ggggttgttt
     4081 tcggccttaa gcaccaccgc tttaatctgc ggccacggcg cggccagctt tttggctgca
     4141 tccaataccg tcatgccctt tacattgagc tgcggcgcgg agcaatccac caaaaaaccg
     4201 gccaaatcac gcccgctcaa gctcaactcg cggccgccct tgcttttgcc gtggcgctgg
     4261 ctgccgatga tgcccgtcat cacgatttgc ccgtctatca ctacctcgca gctctctccg
     4321 cttaaatcgg gtatggccgc ctccggcccc aacctgccga tgacaaaatc gaagctgtcg
     4381 gcagggatta aaaagtcgct gtcgatgtcg tagcgctccc agtggcggtg ctctttaccg
     4441 cccacgcgca ccgacacggc atagccgtat gaattatttt gcatagctgt tgaccaaagt
     4501 gccgcgcttg ataaacgcgg ggtggtggat atggggattg agccgcacca gctctgctgc
     4561 gcgggctata tcgccgtaaa actcgtgggc gatttggtgt atcgtaccgt cgattggggc
     4621 ttggcgcaca atcagcggcg gcttttggtt gatgaccgcc gcaaccaacg cattcagacg
     4681 gcctgccgcc gcgcgcaggg attctgccgt ttggtaagcc tcggtataca cggcgttggc
     4741 cgtcagccca ccagactcgg cagcagccgt ctgcaccgcc cgtaaggcgg caatctcggc
     4801 ctgcatacgg cggcgcatgg cgcggttaac ctcaatcaaa tcgggcgcgg tcatctcttc
     4861 gccatgcgcc tcgattaatg ccgttgccac cgacaacagc gatgacgtgg ataacaggcg
     4921 caccgcctgc gctaccggct gcacctgttt ggcggttaaa cggttcaggc ggttttgcag
     4981 gccgtctgaa aagcggccgg tcagcaaatt atcagggata gcggcggcac ggtcggcaac
     5041 agccgcagcc ccgtcaaacc gctgtcgcgg cgaccaaccg gcatggtgca tggcattatc
     5101 ggccaaaccc gcctcacggc gtatgccagt atctaccatg accgatatat ccgcaaacag
     5161 cttggccgag ccgtttttaa acgccgctgc gctgtatccg ccccgatcgg gaaaggcaat
     5221 tttgtccaaa tcaaacaaac ggcgcaccgc accaaacgta cccgatgccg cactccaaat
     5281 gcccagcgcg ctgccccata aagccgatac gcccgcatcc accgccaaca ccgcatcaac
     5341 aaagccgata gccgcctcgc ggtaggtgtc gatatcagca atcaacgcct caagctcgac
     5401 caaaaaggcg ttttcaaaaa caaagatttc ctgcgcttcg gccgcctcgc ggaaagtaat
     5461 atcgatgccc gcataatcca cataatcggc ctcatgtcgg taactccatg atgccgcaat
     5521 catgttgtgc atccgccccc aaacagggtg caccagcacg ccgccgcccg gctgctccag
     5581 cgcatccagc agctttttca gacggcctgc atagcccttg ccccaaaaca ccgcattaat
     5641 ctgcacctgc cgcccggtca tgcccatgtc ttcaaggtcg ataccctgca caaacggccg
     5701 cgcatgctcg gccaatgcct tgccgttgct ctcgtccacc acctcaatat caaagccgac
     5761 gcccttgtaa gatgcgtcct gcaataaggt atgccatccg ctcattattg acccgctcca
     5821 cggccgaaca tggccacttg ataccgcgat acctcattcg cgataacacg tccgtcgata
     5881 ttcaggctca cattggtatt gatggtctga cttgccgacg ccatcgcact ttgcacttgt
     5941 gccaaacctg ttgtaactgc agccgtatcc tgcgccaatg ctgcctgata cgccgccgtc
     6001 tgctgctgaa tggctgcctg atacgccgcc gtctgagttg aaaacaacgg agacaacatc
     6061 ttatcggtag gctgcgctga cggagcagcc aaatactgag ggacaccacc ccgcgcctgt
     6121 tcccgcttcg gcgcaggttc aaatcggata ccgttgtttt tcagacgatt aatcatttca
     6181 ggagttgtct tggtaccacg caaacccttt ggcaatgtgc cgtcacccaa agactcggaa
     6241 tacagcaaca tacctaacaa acccgcccgt ttaactaacg ctggattatt catgagcccg
     6301 ctaccggcag attttcccca tgacagcaac ttaccgcctg ccgctgtggc acctgctgcc
     6361 acgccgcctg cggaagcctt accccatacc aacgctttac tacctacatc tttcagaaag
     6421 cctgcacctt taccgccacc ggcggttaat aacattgcgg cagacgccgc tgttgccgcc
     6481 gtcgtcaatg cttgcagggc tagagtggca ttagggtatt ccgctgccag ctttttaaac
     6541 gaggtttcca tatccaccaa tgttcccgtc agactttccc gcaacattgc cagcgattcc
     6601 tgttgctctt gttgcgccgc tgccgacaac attcgcgcct cgttgttctt agcaattttt
     6661 ccgttatcca acgttacgcc agccaacgaa gccatatatt cacggatttg cgtcatatcc
     6721 gttgcagcca gcaaaccttg ttttgcctgc aaatcaggca gcagttgcgc caaaagcgcg
     6781 cccttaagca tatttgcctg ctccgccgcc gtcttatcgc ctgcagccgc gcgtttctta
     6841 taatcttggt attgcttatc ctttactagc atggcatcgg caagacggga caacacctgc
     6901 actgcgtttt cgccgttttg cttgccttgc acaaccgagc ctatccaatc gacacctttc
     6961 ttcgggtcat ttggatttgc catcttcttc agacgaccta tcgtgtaagg cgacagagtt
     7021 ttactcaaaa gattttgcac attagtcgcc gcttcggcag gactgcccga tttattcgcc
     7081 gcagattgta agagtgagag caggtagtcc aaaccgccga caccattcat ccctgcctgt
     7141 tgcgcggcag ggagcaggct cggcagctcc cgaaccatat cccgcacctc gaaagtgccg
     7201 tctaaacccg attgcaagac gtgctcaagc ccgagttgca ggtctttacc gctcatgccg
     7261 ccatctttca gggttttaat cagttttgcc gtatcctcgc cactgccttc tgaggcaagt
     7321 gcaaaagcat atgccgcctg cgcttcattc ttggtttggg caaaattcag actggtggtc
     7381 atcatgccgc tgattaaatc caaagcctta tcgtgggtcc cgccattttt ctcgacaagt
     7441 tcaagtgcca aatccttgat ctgttgcgca ccttcagttg caatccacgc tgccgattta
     7501 ctgttatcct caataaatgc ctgtctggac acgcggttga tgttctcatc aagctgcttt
     7561 ctgttgtcca tagcaggctt aagcaccgta tacgctgccg cgccaccagc caccgcagct
     7621 gcaccgaatc ttccgatttt tcccatcttg tccgcaaatc ccgtgccctg tttcagttcc
     7681 gcgttcagct cgcgaattcg gttacgcgta gcgaccgccg cccgtgccaa atcattttgt
     7741 gatgccttgc cgctgcgcgc caaacggtta aacgcagcct gtgtcagcat gatttcgcgt
     7801 tgaatctgtt tttctgagcg gatgccggtc aacgcataag tatggctttg ccgttcaagt
     7861 tgcgcccgcg atttcgcggt acgatctaat tgtcgttgag tatcagccag tagccgtctc
     7921 gctccgtcat ctcgggcggt taacaccaac gacagtttca tgttcccgtt tgccataaaa
     7981 agcagccttt aaacgtatta actcaaggtc aattatcgtt taaaggctgt ttaagggagg
     8041 cggtatgggc ttcaaccccg ttttcgtgct ttatttgttt gttgcgttat aataccagaa
     8101 tgataaccat tttattcaaa gggatatttt atgaatatga aaaaattgat ttccgcaatt
     8161 tgtgtttcaa ttgttttatc agcctgcaac caacaatcaa aaacggcaca agccgaagaa
     8221 cctgtccaaa gtatccaggc tgctgattgt accgccccaa tggacatcac agttgaacaa
     8281 tatctcatca atttggagca agcatttaaa actcagaacg tctcaacaaa aatccataat
     8341 aaaaatattg tcaagaccga ttgtggttat gaccttactt tggtaatgga ttttggggcg
     8401 attgcgctca aactggacga gcagcaaaaa attagagcta tctcagtagg ctacatttta
     8461 aaaaccgacg gagagaaagg acaaaatcta gtcaataatg ccataaatgg attacacagt
     8521 attcaggcag ttctgtcttt aactaccaca gacaaattgg gcgaatcgga agcaggaaaa
     8581 caacttttta cagctttaac cgaagtcgtc aaagaatcca atcagacagg agcaacagcg
     8641 caaaaagacg ttccggcaga tggtatttta tatagcgttg tttttgaaaa agaaacaaac
     8701 accattgcaa taatcggcag aaaacaaccc taaccgtcaa ataaaaaaaa ggtcgtctga
     8761 aactcagacg acctttttat tttccctccg gctttctcag ccgtttcgac acaatcactc
     8821 cctcttcctt cggcgttttt atgcccaggc tcgacaggat gtcttcaaac catgccgaca
     8881 gctcggcgtg gcacatatcg cgcactgcgt ccgcccctat ccccgccttg ccatcaggat
     8941 gaccgcctga cggtagcttt tgtgtgccgt caggtagctg cgttttactg ctccgccgcc
     9001 gacggcgcat cggggctcgc cgtagcggcg gacggtttta ggcgcaaatc ctcctgagcc
     9061 gccatcacgg tatggtagtc ttcggtagag aggttttcca tcagccactg cgccgtcagg
     9121 atgtcggatg ggataccttg ggcttcaatc tgctgcgacc agtaggtcag cgtctctagg
     9181 acgtcgcgtt gcagcagttc ggcttttgtg gcgttttcgg gcagctcggg caagtcatca
     9241 atcagggcga acgccgccag ttcgccgccg acggtcagtg gcttgaggcg taattcagat
     9301 acgactttgc cattgtaggt cagcccgtgg gtcagtttga cggtaatcac gcattactcc
     9361 ttgattttgt gcaaagcaat catctcaatg tcgatgcgtg cctcgttatc gacttcatat
     9421 tgctcgccgg tatcgacggt aaagcagtcg aggtaggaag tgcggcgatc ttcgtcgttg
     9481 atggggtaaa tcgtgatttt cgccttggtg atgttgtccc agtcgatttc cgtaccgtcg
     9541 atcggaatgg cggcggtaac gcttaatttg tgttcggtta cgccgtcaca ataaccgttg
     9601 actcggccgt tgcggttcat cgttttgacc ggcttgcggc ctgtagtggt ttgcggcttg
     9661 atgctcacaa tctcgatatc gcggccgttc atctccataa tcaccgcgtc ggcataggta
     9721 gcgtcgctca tgctttatcc ttttttaagg tttcagacgg cctttaaacc tgttttaaac
     9781 gccgtctgaa aagggttaca aaatcaaatc aatgcgcccg gcaaagacgt gcaggccgtt
     9841 gaccacatcg gcggggataa tggcattaac acggttgggg tcgttttgcg cacgcgccac
     9901 caccagcttg cctttgttgg cctcggcgtt ttcgatgatt tcggcttggt cgagcttaat
     9961 cagcacgtcc aaaatctcgc tcttaacctt gggcagcagg cggtcgctca atttgtcgcg
    10021 cggaaaacgc agggcaatgc gctctttaac gctgcggcgc acataatcca gcgtgcggat
    10081 ggtggtaatg tcgagtagtg ccgggtcgtc ggtgttgttg gccgacttgg tataggtgga
    10141 tacggcacgc ataatctgca cgcggttgtt gaccactgtg agcggggtca agccgttgta
    10201 cagcgcattg ttgcattctg caaacagcgg ccattgcgca tcaggtgtaa cggccagccc
    10261 tttgatttcc agcgtgttca gcggcttggc agggtcttct tcaaaggcca acaccgccgc
    10321 ataacctgcg gcgatgatgc cgtttggctc taccgcacct ttgtaccaag cacaggtcat
    10381 gcggccgtcg ttgatttcgc cggtagcggt ggtggccgtg ctcaaggccg cactcatacc
    10441 caatacgccg atacagccgc gctgctcgat ggcgttggat acgttggtaa tatggttgct
    10501 caaggctttg gcgttggcgt catcgctaaa cgggctgcaa ataatgtgat aatgcttgcc
    10561 cgccactttg gacaaggccg tggcaatgtc cgcatttttg gcaccgccgg taaaggcatt
    10621 ggcttgataa gttaggccgg tattgccggt gctcacggtt aagccgctct cgttgccgat
    10681 ctcgcctttg tgtttggccg tcagcgtaac ggctgcgccg ctgccggatg cggtaacggt
    10741 tacatcggcg gcagtgatgg cggttttcag acggtctgcc actgtggcgg cggtctcgcc
    10801 ggtgttaacg gccgtgctta cctgcttgcc gccaatcgtg atttccacca cgcccggcgc
    10861 ggtggccgtg ccggaaaggg taacggttgc ggttgcctgc acgcctgcgc tgtggtcggc
    10921 aataccgata acggtcaaat ccaaataagg gttgttggca aatgcttggc gcaccatcaa
    10981 atgcgccagc gagccttgtc cgaacaaatc ggccgcctcg gcatcgctaa atagttgcac
    11041 cggctctaag gcgggctgta tgcccgcggt cagcatgggt gcaaccatca ataccttttg
    11101 cggattttgc ggcaaacctt gtacggcatt gcgggtgtta aattcaatat actgcccggg
    11161 cacgcggatg ctgcccggaa tcgtgtcaaa atcaatatgg ggcatcatta ctctccctta
    11221 gtattgcggg ttttggtatt tggggcggca tcctcaacca ccaccaaatc gccgtcatca
    11281 atcatgcggc ggtaatacag gctgttgccg tccacctcca ccggctcttg gccgatatat
    11341 tcgtgcgggt tgtgttcggt cggcacacgc aggccgtctg ccgccgttac tttgattttg
    11401 ctcattgctt atccttaagg gttaggttta caggtatttc atcggcggat tgcgggtcgt
    11461 aaattttgcc gtccaacccc tcgaaatcag gccacggctc gctcaatgta ccctgatact
    11521 tggtaaagat atggttgggg tcgtcgggat tgtcggtgcg ttcggggtag cggtcatttt
    11581 ccaacccgca ggtgttaaag cggatggcat actcaaccgc atatatgctt actgcggcgt
    11641 tttgcaccag cacatgattg gcaatcgcgc gcaccgcttt gggcaccaag ccgcggctat
    11701 cggcaaaacc gagccgctgg ccgtcaagca ggcggcgaac agcgcggatt aaatcgttgc
    11761 tgccgatttc gcggctgtcg atgccgcctt gccgctgcgc ctgctcgttg cgcagattgc
    11821 gggccgccac catcaccaca aattcggcgg tatcctgata acgtccgcat acgccgccgg
    11881 tgctggcagg ctcaactttg ctgccgccat acgttaccca aaccgcaggc agcgtatgga
    11941 tttgccccgc caaatcgtcg gcctcgccgt tgtagctttt aaccgtgcgc accatgcgac
    12001 ccaagccccg tttcagacgg tctgttatcg cttgctcaat atcagtgatc acgcccgaac
    12061 accttatttt taccgtttag aaacacaata ccgtcttcgc ccgcggccac ttcttcgccg
    12121 ctatcatcca cgcccagctg cacttcgcct ttggccatag cacgcagcag gtcgagcacg
    12181 tcgattttgt agcggttgcg gatttcgtcg gtaatcaaca cgccctgagc cgccgtcaga
    12241 cggtagcggg caatgtcgca gcataggctc ggcgcagatt gccttccaca ccgcttcgcc
    12301 gacttcggcg cgcttcactt cgcgccagcc gccgtcaaac agcaggccgc cgcgccaaaa
    12361 ttctttgccg tctgcgccgg tactgacgag catcacatcg cggctgtccg ccaaagcgtc
    12421 ggcggcacgt tgcgtatgct gcactttgag ttcggcaagt tcggcggaca gtgccttttt
    12481 gtcgtcttcg gctttttcca aggctgtggt cagcatttcg acatcgtttc gggcggcggc
    12541 aagctctgcc tgcacggcgt ccaattcggc tttgatgtct tcaaacgacg gggcggcggt
    12601 ttcggcggtt tctggtttgt tgttggtttt tgccatgatg actccttgtt tcagacg
//
LOCUS       DAWKQB010000051        12224 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.051, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000051 DAWKQB010000000
VERSION     DAWKQB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.051"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..175
                     /locus_tag="WJL14_001049"
     CDS             <1..175
                     /locus_tag="WJL14_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ7656130.1"
                     /translation="DGGRSQISAESKVAELENKIIPGMPKNERLKIKQKIRNITKNAN
                     RKAKGEEHGRRGR"
     gene            156..395
                     /locus_tag="WJL14_001050"
     CDS             156..395
                     /locus_tag="WJL14_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656131.1"
                     /translation="MVDAVVKTPEFVPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     RTEYYESPDSNLLIAVKKKQNYFDILLSGAIFYGR"
     gene            385..612
                     /locus_tag="WJL14_001051"
     CDS             385..612
                     /locus_tag="WJL14_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656132.1"
                     /translation="MGVNLIGLNFEDFKQLFHHPKSYGVDEYYLSNESYPTYVYEFDE
                     IGVQAWEAKGKVVTIIAGGKDNYSTEPYYDE"
     gene            619..675
                     /locus_tag="WJL14_001052"
                     /pseudo
     CDS             619..675
                     /locus_tag="WJL14_001052"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            681..1058
                     /locus_tag="WJL14_001053"
     CDS             681..1058
                     /locus_tag="WJL14_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ7656133.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFT
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            1122..2255
                     /locus_tag="WJL14_001054"
     CDS             1122..2255
                     /locus_tag="WJL14_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656134.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRL
                     KEKAKWFHVTEQEHGEEVWLNYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTVHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADMQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(2342..2617)
                     /locus_tag="WJL14_001055"
     CDS             complement(2342..2617)
                     /locus_tag="WJL14_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656135.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKIRLMCISVFWIVVFNL"
     gene            complement(2634..3401)
                     /locus_tag="WJL14_001056"
     CDS             complement(2634..3401)
                     /locus_tag="WJL14_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ7656136.1"
                     /translation="MRPYATTIYQLFILSIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACHLNQDLGKSLLDLGFKKIPSVYPE
                     YEAYEDKRSIPENPYIHELQYIKKGENPAIITHQNYHRYGENDYSTDIGSCINGFTVQ
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3788..4063)
                     /locus_tag="WJL14_001057"
     CDS             complement(3788..4063)
                     /locus_tag="WJL14_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7656137.1"
                     /translation="MNASNWSLYLILCENSAFYCGISPNPQQRLAAHTAGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(4060..5343)
                     /locus_tag="WJL14_001058"
     CDS             complement(4060..5343)
                     /locus_tag="WJL14_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7656138.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGHFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFVLALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5498..6916)
                     /gene="glnA"
                     /locus_tag="WJL14_001059"
     CDS             complement(5498..6916)
                     /gene="glnA"
                     /locus_tag="WJL14_001059"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ7656139.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDAGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDTLVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(7190..7576)
                     /locus_tag="WJL14_001060"
     CDS             complement(7190..7576)
                     /locus_tag="WJL14_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656140.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVQVGIDSDNNTTFGDADSRTD
                     NRARFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPS
                     YAEWNYVIPGTLGEAQYKFVCNGYPR"
     gene            7747..8556
                     /gene="aroE"
                     /locus_tag="WJL14_001061"
     CDS             7747..8556
                     /gene="aroE"
                     /locus_tag="WJL14_001061"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ7656141.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAL"
     gene            8559..9260
                     /gene="mtgA"
                     /locus_tag="WJL14_001062"
     CDS             8559..9260
                     /gene="mtgA"
                     /locus_tag="WJL14_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ7656142.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(9463..9538)
                     /locus_tag="WJL14_001063"
     tRNA            complement(9463..9538)
                     /locus_tag="WJL14_001063"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9503..9505),aa:Phe,seq:gaa)
     gene            complement(9729..10463)
                     /gene="lptB"
                     /locus_tag="WJL14_001064"
     CDS             complement(9729..10463)
                     /gene="lptB"
                     /locus_tag="WJL14_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656143.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(10537..11067)
                     /gene="lptA"
                     /locus_tag="WJL14_001065"
     CDS             complement(10537..11067)
                     /gene="lptA"
                     /locus_tag="WJL14_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ7656144.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(11048..11629)
                     /gene="lptC"
                     /locus_tag="WJL14_001066"
     CDS             complement(11048..11629)
                     /gene="lptC"
                     /locus_tag="WJL14_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ7656145.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(11626..12162)
                     /locus_tag="WJL14_001067"
     CDS             complement(11626..12162)
                     /locus_tag="WJL14_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7656146.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         3145 a         2992 c         2984 g         3103 t
ORIGIN      
        1 agatggcggt cgaagtcaaa tatcagcgga atcaaaagtt gctgaattag aaaataaaat
       61 tattcccgga atgcccaaaa atgaacgctt aaagattaag caaaaaatca gaaatattac
      121 aaaaaatgcc aatcgaaaag caaaaggaga agagcatggt cgacgcggtc gttaaaactc
      181 ccgaatttgt accttttact tctgtaggaa tattccgttt tggtgcggat ataacacaat
      241 acaaaaacat actggaaact tttatgtatg aacctcctga tgagtttaga acagaatatt
      301 atgaatctcc cgactcaaat ttactcattg ccgtcaaaaa aaaacaaaat tatttcgata
      361 ttttgttatc aggagctata ttttatgggc gttaatttaa taggtctaaa ttttgaagat
      421 tttaaacaac ttttccatca ccctaaatca tacggtgttg atgaatatta tttatcaaat
      481 gaatcttatc cgacatatgt ctatgagttt gatgaaatag gggtacaagc atgggaagca
      541 aaagggaaag ttgttactat cattgccggg ggtaaagaca attattcaac agaaccttac
      601 tacgatgaat aacattacag aaaagaaaca agaaacggta catttgataa aaacctaaat
      661 cgtattggag attaattatt atgtgtgagt tcaaggattt tagaagaaac atcccttgtt
      721 ttgaagagta tgacgaaaat tcatttattg gcaaatggta tgatgacggg gtgtgggatg
      781 atgaagaata ttggaaattg gagaatgatt taatcgaggt tagaaaaaaa tatccttatc
      841 cgatggatat accaagggat attgtgattg gaatcggtac cattattgat tttttaatgg
      901 ttccaaattg ggagcttttc gaaattaaag cttccccttg gctacctgat agcgtgggaa
      961 ttcatgaacg ttatgaaaga ttcacaacaa tgctccgtta tatttttacc gagaaagaca
     1021 tagtcaacgt gcgatttgat tattacaaca aaaaatagca aaacccggca atcaaagctc
     1081 gtctgatata atcgatatat tctgttaaac gaaaaataaa tatgtttaaa aaattcaaac
     1141 cggtactgtt gtcatttttt gcacttgtat ttgccttttg gctggggacg ggtattgcct
     1201 atgagattaa tccgcgttgg tttttgagcg atacggcaac tgaagtacct gaaaatccga
     1261 atgcttttgt ggcgaaactt gcccgcctgt tccgaaatgc cgacagggcg gttgtcatcg
     1321 tgaaggaatc gatgaggacg gaggaaagtc ttgccggagc tgtggatgac ggtccgttgc
     1381 agtcggagaa ggattatctc gcgctcgctg tccggctcag tcgtttgaaa gaaaaggcga
     1441 aatggtttca cgtaacggag caggaacatg gggaagaggt ttggctgaat tactatatcg
     1501 gcgagggcgg tttggttgcg gtttcgcttt tgcaacgctc gccggaagcg tttgttaatg
     1561 ccgaatatct gtatcggaac gatcgtccgt tttctgtaaa tgtgtacggc ggaacggttc
     1621 acggggaaaa ttatgaaacg acaggagaat atcgggttgt ttggcaaccg gacggttcgg
     1681 tatttgatgc gtcggggcgc gggaaaatcg gggaagatgt ttatgagcat tgcctcgggt
     1741 gttatcagat ggcccaggta tatttggcga aatatcggga tgtcgcgaat gatgagcaga
     1801 aggtttggga cttccgcgaa gagagtaacc ggattgcgtc ggactcgcgc gattctgtgt
     1861 tttatcagaa tatgcgggaa ttgatgcccc gagggatgaa ggcaaacagt cttgtggtcg
     1921 gctatgatgc ggacggtctg ccgcagaaag tctattggag tttcgacaat gggaaaaaac
     1981 gccagagttt cgaatattat ttgaaaaacg gaaatctttt tattgcacaa tcttcgacgg
     2041 tagcattgaa agcggatggc gtaacggcgg atatgcagac ctatcatgcg caacagacgt
     2101 ggtatttaga tggcgggcgg attgtccgcg aagagaaaca gggggacaga ctgcctgatt
     2161 ttcctttgaa cttggaagat ttggaaaaag aggtgagccg ttatgcagag gctgcggcga
     2221 gacgttcggg cggcaggcgc gacctttctc actgaacgca aatgccgtct gaaaccggtt
     2281 ttcagacggc attggagagt caaacgcttt ttgcaatgcc gtatatgccg aatccgattc
     2341 gctacaaatt gaaaaccaca atccaaaaaa cggaaataca cattaaacgg atctttttac
     2401 cgaccgtatc atcccatagg ttttccaccc cttttttcat ccataaattc aaaccattta
     2461 agtttgcatc cttaccgaca attgcagcag catcatccgc ataacggtca cccaaattag
     2521 ccaaataacg ccaaccgcca taggtatcgc cacggaaagc ataaccacgc aaggtttgga
     2581 tttgttctgg tgttaaaaca acttcaccct cattcataat aagatccttt aaattatttt
     2641 ttacttgaat tgtttacaaa actctgtacc aattgctcta cttgttggtg ataacctacc
     2701 aactcctgct gtgtgagctg ctgcttttcc cgaataaacg ggtaatactg taccgtgaaa
     2761 ccgttaatac aggaacctat atcagtactg taatcgttct ctccatacct atgatagttc
     2821 tgatgagtaa taatcgccgg attttctcct ttcttaatat actgtagttc atgaatatat
     2881 gggttttcag gaatcgacct tttatcttca taagcttcat attcaggata gacagacggg
     2941 atttttttaa atcccaaatc aagcaacgat tttcctaagt cttgattcaa atgacacgca
     3001 tcttttcgga taacaatatc gtccacgcta tccaaaccat cgcgtagaaa ttgaataaag
     3061 tgtgctttgt tctgctggtt gtatggctca agaacccagc cttggtcggt ttttttgtaa
     3121 tagacgctaa tctctatcaa attaatggag taagcatcct gcccgggcgt gtcggaaagc
     3181 cattttgtgg cattgtcttg cgtataccgt gcaagatagc cataagggat aaccattttg
     3241 gtgccctgaa aatcaaatgt aaccgtatgt tcaaatcctg tcattggctc gggattgttg
     3301 aaactggttt gttcattaac aggttcacat gaggtcatag taaaaacact cccaatagac
     3361 aaaataaaaa gttgataaat ggtagtagca tatggtctca taatttcaag cttagaaatt
     3421 agttaaagaa tatattcatg gtagatacta actctttaag ttgacatatt gtgcgatgtc
     3481 gagggctgta ccgtattttc ccatcgtatt ttcccgttct ttatatgagc ggcaggcatc
     3541 ctcataaaag aagggatacc gataaggaaa aataccccgg cataaaaaat atactgatta
     3601 atcaatacaa atccaacaaa cagttcatcg tattcttttc tgttggcgta taaaaaatac
     3661 tgatatatgc cttcactttc atagggcgac gctttatgtg ccgtcctgtg tgttgaaacg
     3721 tcggttttcc ttaccgcctg ccccgctttt ccctgattgc tgccaactgc cgtccgaacc
     3781 tgaaccgtca ggtttcagac ggcatttttt ctgcctgttc ccacaatttc cgtttttgtg
     3841 cggcggtcag ttttttgacg gcgatttcct gcctgagtgc cgtgccttta tccatcccgc
     3901 ctgcaacgat acgcatcgcc accggtttga atacgcgggt atatttcgcg ccttttccgg
     3961 ccgtgtgggc ggcaagccgc tgttgcggat tcgggctgat gccgcaatag aacgcgctgt
     4021 tttcacacaa tatcaggtaa aggctccaat ttgacgcgtt catctgcctg catcctgagt
     4081 tttctccccg ttccaaggcg caactttcag cagcagcagc atacccggta gggcaagtac
     4141 gaaacacagt tggaaaaacg gtacataacc gagccattca atcagataac cggcaaagga
     4201 attgatgacc gtacggggaa cagcggacag gctggtaaac agcgcaagct gcgttgcggt
     4261 aaatgcggga ttggtctcgc gcgccatata cgatacgaac gccgccgtcc ccaaccccac
     4321 gccgaccgct tccgcgccga taactgccgc cagcatcagc ctctcgcctg tgccgaccgt
     4381 gtcgaaatgt ccgaaccctg ccagccatac aaaccccaaa acggttacag cctgcaccgc
     4441 gccgaatagc cacaaggctt tgtttacgcc gattttcagc atccacacgc cgcccaagat
     4501 acctgccgcc actgccggcc acagtcctgc attttttgca atcaaaccga tgtctgtctt
     4561 gctgaaaccc atatccagat aaaacggcgt tgccaacgcg gttgccatac tgtcgccgag
     4621 tttgtaaagg aagataaaca gcagcacgca caccgccgaa gcgatgccct tgcgcgtaaa
     4681 aaattcttta aacggctcta ccacggtctg cttcaacgtt ttaggaacgg caggaggcaa
     4741 cacgggttcg cgcgcaagaa acagcgtcat cagaaggccg ggcagcataa ataatgaagt
     4801 gataacaaat acttctgacc acggcatcct gtctgccaac accaaactca atgaaccggg
     4861 aatcagggcg gcaacccgat aggcgttcac atgaaccgag ttacccaaac ccaattcttc
     4921 gtctgacaaa atctcgcgcc tgaacgcatc caatacaatg tcctgactgg cggaaaaaaa
     4981 agcgacaagc accgacaagc cggcaatcag cggcagatga ttacgggggt ttaaaaaggc
     5041 atatgccgcc aaagctgcca gcaaacctgc ctgcgtcagc agcatccacc cgcgccgccg
     5101 ccccaaaacg ggcagcctga ccgcgtccat cagcggcgac cacaaaaatt tccaagtaaa
     5161 cggcagaccg attaacgcca tcagcccgat gctcttcaaa tccacctgct cgctgcgcaa
     5221 ccacgccgga atcaggttaa tcagaaagta cagcggcagc cccgaggtaa accccgtaaa
     5281 aatacagaca agcatattgc gggaaaagat ttgcccgata aaacctgatt tcgatgcagt
     5341 catcgttcgt gcagcccaaa aaaacaaaag cctattgtag caaatatcgc ccgcctgctg
     5401 aaaaacgccg ccccgcgccg ccggtttgcc gttcaaatgc aaaatgccgt ctgaaactgt
     5461 gttcagacgg cattgctttt ctgaaaaccc gacgtgctta caggctgtaa tacatttcaa
     5521 attccagcgg gtgaggtgcc atgcggatgc ggcgtacgtc ttcttctttg aaagcgatgt
     5581 agctgtcgat ccagtctttg ctgaacacgc cgccgcgcag gaggaattcg tggtcggcct
     5641 tgagggcggc gagtgcttct tccaaagaag cgcaaacggt cggcaccaat gtatcttctt
     5701 ccggcggcag atcgtagagg tttttatcgg caggatcgcc cggatggatt ttgttttgaa
     5761 taccgtccaa accggccatc aacaaagcgg cgaacgccaa gtatgggttg gcggtcggat
     5821 cggggaaacg cgcttcgatg cggcgcgcct tgctgctgtt cacggacgga atgcggatgg
     5881 aagcggaacg gtttttggcg gagtaggcga gtttggtcgg cgcttcaaag tgcggcacga
     5941 ggcgtttgta ggagttggtg gacggattgg taatcgcgtt cagggctttg gcgtgtttga
     6001 tgatgccgcc gatgtaataa agggcggtgt cgctcaaacc ggcatagccg tcgccggcga
     6061 acaggttttg accgtctttc cagatggatt ggtgaacgtg cataccgctg ccgttgtcgc
     6121 ccataatggg tttgggcatg aaagtggcgg ttttgccgaa gttgtgggca acgttttgaa
     6181 tcacatattt catgtcttgg gtttggtcgg cgcgtttgac caaggtggcg aaacgcgtgc
     6241 cgatttccat ttggctgccg gtaccgactt cactgtggtg gacttcgact tcgatgccga
     6301 gttcttccaa aatgtttacc atcgcggagc gcaggtcttg tcccgcgtca atcggagcaa
     6361 cgggcgcgta accgccttta acggcagggc ggtggccggt gttttgaccg tccatgtgca
     6421 ggccgcttgc ccatgcgccg ctttcggacg tgatttcgta acgggttttg tgcatgtcgg
     6481 tttcaaattc tacgccgtcg aagacgaaga attcaggctc ggggccgaag tatgccgtgt
     6541 cgccgatacc ggaagatttc aaataggcct cggcgcggcg tgcgatggag cgcgggtcgc
     6601 ggtcgtaacc ctgaccgtcg gcgggatcga taacgtcgca agtcaacaca acagtcgcat
     6661 catcataaaa aggatcgacg aaggctgtag acgcatcggg acgcaactgc atatctgaag
     6721 cctgaatacc tttccaaccg ccgatggacg aaccgtcaaa cgcctgtccg ttttcaaacc
     6781 actcttcggg gtcttccaac acgatgcgcg caggcacggt aaagtggtgc tgcttgcctt
     6841 tggtatcggt aaagcgcaaa tcgacaaagc gggcttcgct ttcttcaatc aattttacgg
     6901 cgtttttaat agacattttt cagctcctgg aaaagggaaa tagcaaaata tgcggaacgc
     6961 ggcagattag ctcaaagcca gcatgattct accacgtcca tgccgtctga aactggccct
     7021 gcgtcaaatt ccaagctgta aaatatagtt tcagacggca agattgtcaa caatatgctt
     7081 ctgtttgtct gttcaaaagc ggaatacccg cccttttgtc cgaatgccga caaaacagat
     7141 gccgtctgaa agcagttcag acggcatctg ttgtttggtt tgaaaacggt cagcgaggat
     7201 agccgttgca gacaaatttg tactgagcct ctccaagcgt accgggaata acataattcc
     7261 attcggcata ggatggtgta taagaagcga tagagtttcc cgatttggaa taccacgttg
     7321 ccgtcaatat cctaaaacgt ttaccactgc aatccatttc ttccaacgtt ttagctgtat
     7381 taaaaaattt tccattgcca tacttttgcg gcttggcgaa ccttgtttca aaccgtgctc
     7441 tgttgtccgt acggctgtct gcatcaccaa acgtggtatt attgtcactg tcaataccta
     7501 cctgcaccca atccgccgcc accgccggtg ttgccaaccc caatgcaaat actgccaagc
     7561 acaacatctt tttcatgtcc gaccctctcg tccttgtcaa caaaataaag ttaaattgta
     7621 aaaaaaaacg acttttttca agagaaaaat attccgtcat gctatctgac aaccgtttca
     7681 gacggcatcg tttccatctg tacgccgccg ttataaaata gccttcccgt cttttggaac
     7741 acgaccatga ccgccctccc ccgttactcc gtttttggca accccgttgc acacagtaaa
     7801 tcgccgcaaa tccatcagca atttgccctt caggaaggcg ttgacattga atacgaacgc
     7861 atttgcgccg acatcggcgg ttttgcacag gcggtttcga cattttttga aacaggcggt
     7921 tgcggggcaa acgttaccgt accgttcaaa caggaagcgt ttcatctggc ggacgagcat
     7981 tccgaacgcg cattggcggc aggtgcggtc aatacgctga ttccgttgaa aaacggcaag
     8041 ctgcgtggcg acaacaccga cggtatcggt ttgaccaacg acatcacgca ggtcaaaaat
     8101 attgccatcg agggcaaaac cattttgctt ttgggcgcag gcggcgcggt gcgcggcgtg
     8161 attcctgttt tgaaagaaca ccgtcctgcc cgtatcgtca ttgccaaccg tacccgcgcc
     8221 aaagccgagg aattggcgca gcttttcggc attgaagccg tcccgatggc ggatgtgaac
     8281 ggcggttttg atatcatcat caacggcacg tcgggcggtc taaacggtca gattcccgat
     8341 attccgcccg atatttttca aaactgcgcg cttgcctacg atatggtgta cggctgcgcg
     8401 gcaaaaccgt ttttagattt tgcacgacaa tcgggtgcga aaaaaactgc cgacggactg
     8461 ggtatgctag tcggtcaagc ggcggcttcc tacgccctct ggcgcggatt tacgcccgat
     8521 atccgccccg ttatcgaata catgaaagcc ctataaacat gttccgcatc atcaaatggc
     8581 tgattgccct gcccgtcggc atctttatct ttttcaatgc ctatgtgtac ggcaacatca
     8641 ttacctaccg cgccgtcgcg ccccatcgga ctgcctttat gtcgatgcgg atgaagcagt
     8701 ttgaacagga aggtcgcgat gtcgccctgg attaccgttg ggtaccctac aaccgcatct
     8761 cggtcaacct gaaaaaagcc ctgattgctt ccgaagatgc ccgtttcgcc ggacacggcg
     8821 gcttcgattg gggcggcatt caaaacgcca tcaggcgcaa ccggaacagc ggcaaagtga
     8881 aggcgggcgg ttcgaccatc agccagcagc ttgccaaaaa cctgttttta aacgaaagcc
     8941 gcagctatat ccgcaaaggc gaagaagcgg cgattaccgc gatgatggaa gccgttaccg
     9001 acaaagacag gatttttgaa ctgtatttaa actcaatcga atggcactac ggcgttttcg
     9061 gcgcggaagc cgcgtcccgg tatttttatc aaatacccgc cgccaagctg accaaacagc
     9121 aggcggcaaa actgacggcg cgcgtccccg ccccgctcta ctacgccgac catccgaaaa
     9181 gcaaacggct ccgcaacaaa accaatatcg tgctcagacg catgggttcg gcagagttgc
     9241 ctgaaagcga cacggactga gtgccgccgc aatataaaat gccgtctgaa atatcattca
     9301 aacggcatat gctttctgcc tttaccctct tctccaaaac gcaacggggc ggtcattatc
     9361 atttttgccc ttctacataa cggtcagaca caaaacgtgt ccgacacgga caacttccca
     9421 aaaacggcag gcaaaaaaaa taaccgcttc aaggcggttt tttggtggcc agaggcggaa
     9481 tcgaaccgcc gacacacgga ttttcaatcc gttgctctac caactgagct atctggcctt
     9541 tgtctgtctc gttaagagat gcgtattaaa ccaaattacg ggacgacatg caagcatatc
     9601 gggcaatatt ctttgaaaac aggtttaagt caatgtttta aatttgaatt aatttatgta
     9661 aaaacaacat aaatccgtat ttttgccgcc tgcccgacga tattaggtcg cccgtctgaa
     9721 aaatattttc aatatttgaa gttcttaccc agataaacag aacgaacctg ttcgtttccg
     9781 accaaatcat caggttttcc cgatgccaac accgtgccgt ctgaaataat gtaggcccga
     9841 tcgcagatgc tgagggtttc gcgtacgttg tggtcggtaa ttaatacgcc gataccgcgc
     9901 gacttgagaa atccgatgat tttttggatg tcgatgacgg cgatgggatc gacgccggca
     9961 aaaggctcgt ccaacaaaat aaaatgcggt ttcatggcaa gtacgcgggc gatttcgacg
    10021 cgtcggcgtt cgccgcccga caaagatggg gcaggattat tgcgcaaacg ctcgatattc
    10081 aggtcggcaa gcagtttttc aacttcgccg tcgatgcggc ttttgtcttt ggtactgatt
    10141 tctaaaatgg cgcggatgtt ttgttcgacg gtcattttac ggaaaataga ggcttcctgc
    10201 ggtaggtagc cgacacccag gcgggcgcgt tcgtgtatgg gcaggtgacg caattcctgc
    10261 ccgtccagca tcacgctgcc cgcatcggcg gcaatgagtc caacaatcat gtagaagctg
    10321 gtggttttac ccgcaccgtt gggcccgagc aggccgacga cttccccgct ttcgatttca
    10381 agggagaagc ttttaacgac ttggcgtttt ttgaaacttt tttgcaggtt ttgaacaaca
    10441 aggcggctga cgtttgcgct cataaatatc tcttcaatat cttcggtcgg caaatgccgt
    10501 ccgaaccagg tttatgtttc agacggcatt ttgggattat tcggattttt gcgtactcga
    10561 aggctggata acgacgctga ccctgccgga tttggaagcg gattttgcgc cggattttgt
    10621 gctgccgctg atggtataga cttcggtttt ggtgttgtat gtaatcaccg caccttcggc
    10681 gacatcgccg ccgcgctgta ctttggcatt accggttaag actacggtgc tgcctgcaga
    10741 tgaataagca acgttgtttg cctgtccgcg caccgtgcct ttgccgccgt ccaatgtctg
    10801 gctgaagcgg actggcgaac cttccgccct cacggattcg ccgcctttgc cgccgcgtgt
    10861 aacattgacg cgggcggcgg aaatattgag cgtaccctgt ctgatgacga cgtttccgct
    10921 gaatgtggtg ctttggttgg cttgatcgag cgaaccttgg tcggcctcaa tctgaatagg
    10981 ctgcctgctg tcgctttgaa gggcaaaagc gggggacgcc gaaaaaaatg ctattaaaac
    11041 aaatagctta catatctttt gtatcataaa tcgtggcttt cactttagat gagaagttca
    11101 acatgcctgt tttgtggtcg taagtcatgc cgcccgcctg accgtgcgat gcaccatatt
    11161 ggaaactgac aggcgtatcg gtttgggcat attgagattc ggtatcgacg tgcagctttt
    11221 cggcttcaac tttacccgcc tgccgtttgc cgtcggcggt tttggtcagc acaacgttgt
    11281 ttttaaaaag aacctgtttg ttttcggtat ggtaaacggc ttcatcgctg ccgacttcgt
    11341 acaacaacct gccttcttgg aagaacacga gatgcggtga gtcgaaatga atgtcgctgc
    11401 tttcgggaaa ctgtttcgcg cccttcgaac tcaaatgttc tttcaagtat ccctgttcgt
    11461 caaaccgcct gccatccaat ccgtccattg tgtattgcgg ttcgtcggga ttgagcctga
    11521 cttcttcaat ctcgacttcg ctgatgcgtc ccaaccatgc cgacaggctg cccaaggcaa
    11581 ccgccaatat caatgggaac gcaattccgt accgccatct tactttcatt tgatgtactc
    11641 gttcaaagcc gcgcccaaag tcccttgcgc ctgcataatc aggtcgcaca cttcgcgcac
    11701 cgcgcccgca ccgccggggc gtttggcaat ataggcggcg tgttgcagcg taaaccaatg
    11761 cgcgtcgggg acggcaaccg gcaatccgca gcgtaccata acgggcaaat cgaccacgtc
    11821 gtcgccgaca aaggcacact cggcttcttc cacgccgacc tgagcgcgca gctcttcata
    11881 cgcggcgcgt ttgtccgaaa tgcctttgaa gtagtaattt atgcccaact gtttgacgcg
    11941 gatgccgacg gagggcgcgt cccgacctgt gataatcgca gtctgcacgc cgcttgcctg
    12001 aagcattttc agaccgtgtc cgtccagtgt gtgaaacgat ttgatttctt cgccgttatc
    12061 gcggataaag atgcgcccgt cggtcaaaac gccgtccaca tccaggatca acagtttgat
    12121 tttggcggcg cgcgcctgta attcgggaga aattgcctgc atgggatttc cttgttttca
    12181 gacggcatat cttaccacaa atgccgtctg aaacggctgc aacg
//
LOCUS       DAWKQB010000052        11969 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.052, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000052 DAWKQB010000000
VERSION     DAWKQB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.052"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            142..576
                     /locus_tag="WJL14_001068"
     CDS             142..576
                     /locus_tag="WJL14_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ7656147.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(624..1967)
                     /locus_tag="WJL14_001069"
     CDS             complement(624..1967)
                     /locus_tag="WJL14_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ7656148.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYAHLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGAWLDNQDTLKAIEHTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1972..2625)
                     /locus_tag="WJL14_001070"
     CDS             complement(1972..2625)
                     /locus_tag="WJL14_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ7656149.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQVRPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2776..3066
                     /gene="gatC"
                     /locus_tag="WJL14_001071"
     CDS             2776..3066
                     /gene="gatC"
                     /locus_tag="WJL14_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ7656150.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3129..4574
                     /gene="gatA"
                     /locus_tag="WJL14_001072"
     CDS             3129..4574
                     /gene="gatA"
                     /locus_tag="WJL14_001072"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ7656151.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGIPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQKLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFS
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPSAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSSGGLPIGV
                     QLIGNYFSEAKILGAAHQIQLNSDWHGKRPE"
     gene            4571..5503
                     /locus_tag="WJL14_001073"
     CDS             4571..5503
                     /locus_tag="WJL14_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ7656152.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5546..6976
                     /gene="gatB"
                     /locus_tag="WJL14_001074"
     CDS             5546..6976
                     /gene="gatB"
                     /locus_tag="WJL14_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ7656153.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAHDYRYFPDPD
                     LLPVIISDDRLQKAKAEMPELPKEMAARFVADYGVSDYDARLLTASRVQAAYFEEAAK
                     ESGQGKLTANWMNGELAATLNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEASIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7068..8078
                     /locus_tag="WJL14_001075"
     CDS             7068..8078
                     /locus_tag="WJL14_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ7656154.1"
                     /translation="MNHTITLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMGRHSEQALSEAEKAQGKILMCCTTAQSDISINIPGYKADALPVRTLP
                     ARIENIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQGSSRVVHFYWGARHQDDLYALEEAQGLADRLKNACFTPVLSRPRECW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WJL14_001076"
     CDS             complement(8208..8840)
                     /gene="pdxH"
                     /locus_tag="WJL14_001076"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ7656155.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9061..10110
                     /locus_tag="WJL14_001077"
     CDS             9061..10110
                     /locus_tag="WJL14_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ7656156.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(10179..11720)
                     /locus_tag="WJL14_001078"
     CDS             complement(10179..11720)
                     /locus_tag="WJL14_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ7656157.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNTFSNATSVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMAGLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMIFVKA
                     LDLFS"
BASE COUNT         3072 a         3603 c         2972 g         2322 t
ORIGIN      
        1 ttttgggaat ctgtttcacc ccaatatata aagcaccata ttaaggcgga gtgtcttccc
       61 tgctttgccc cgaacccgga aaagacaccg cccgagccaa tcccgattct gccctggcag
      121 tcagccatta aggaaatcct aatgaacttt gctttatccg tcattatgtt gaccctcgcc
      181 tctttcctgc ccgtcccgcc tgccggagcc gccgtcttta cttggaagga cggcggcggc
      241 aacagctatt cggatgtacc gaaacagctt catcccgacc aaagccaaat cttaaacctg
      301 cggacgcgcc aaaccaaacc ggcggtcaaa cccgcccaag ccgacgcagg gaagcgcaca
      361 gacggcgcgg cacaggaaaa caatcccgac actgccgaga aaaaccggca gcttgaggaa
      421 gaaaagaaaa gaattgccga aaccgaacgg cagaacaaag aagaaaactg ccggatttca
      481 aaaatgaacc tgaaagcggt gggaaattca aatgcaaaaa acaaggatga tttgattcgg
      541 aaatacaata acgccgtaaa caaatactgc cgttaatcgg ctctagcgca aacccgatgc
      601 cgtctgaagc ggcacggggt ttgtcatttc tgccagtagg ttttgacgtt gacgaactcg
      661 tacagcccga attcggacaa ttcgcgcccg taaccggaat ctttgactcc gccgaaaggc
      721 aggcgcaaat cgctgctggt atggcggttg ataaacaccg atcccgcctg tattttttcg
      781 gcaaaccgcc aagcgcgttc ggtatcggcg gtataaatgc aggcacctag cccgaacggg
      841 gaatcattgg caagggcgat ggcatgttct tcgttttcgg cgcgcaaaat cagggcggcc
      901 ggcccgaata cttcttctct ccagacgcgg caggcaggat ttaccctgtc taaaaccgtc
      961 gcgggataaa accagcctcg cccttgtggg atttttccgc cggtcaggca taccgcgccg
     1021 tttgaaacgg catcttcaac ctgcccgtga accctgtccc gcaaatcttc gcggtgcagc
     1081 ggtgcaagcg tagtatcggg atgtttgggg tcgcccattt tcaatttggc gcattcggca
     1141 agaaacagcg tgataaaacg atcggctgcg gcttcggtta cgatgatgcg cttggcggcg
     1201 ttacacgatt gccccgcatc gcggaaacgg gaataacagg cttctgcggc ggcacgctcc
     1261 aaatcggcat cgggcatcac gataaaggcg ttgctgccgc cgagctccaa gacggttttc
     1321 ttgaggtttg cgcccgcgtg tgccgcaagg atgcgccccg tatgcgtcga accggtaaac
     1381 gccattgcat cggtgtgttc gatggctttg agtgtgtctt gattgtccag ccacgcgcct
     1441 gccagaggaa tgccgtctga agccaaatcg aacagtgcct gactgacgcg tgccacgctg
     1501 ggcgcgggtt tgacggcgca cgcgttgccc gcgcacatag cgggaacggc gaaacgcaat
     1561 acctgccaga cgggatagtt ccaaggcatg acggcaaaca ccacacccaa aggctcaaac
     1621 cttacctgac tcaaactcgc ctgcgtcgcg atggttttgt gggcaagcag ttcgggggca
     1681 aggtgggcgt aatagcgtat cagttcgata gacttgccga tttccgcacg gcattcgtgc
     1741 aggcagcgtc cgacttcttc gcacaccatt tccgcaaaac gctctttctc cgcctccaaa
     1801 cggtcggcaa atttttgcag acgcgcggca cgttcggtta cgcccagttg cgcgaacgcc
     1861 ccgccgcgca ttttcaaatc cgccagccgc cgttcaaact ccgcataatc ttgagcgggg
     1921 cggcggtaaa gcgtttcgcc cgtaaataca ttgacactgt gaaacatcga atcaacctgc
     1981 cagttgcggg aatatcgttt tcagtcccga cacaataatc tccaccgata ccgccgccag
     2041 catcataccc atgatacggt ttaaaatcgt cagccccgtc gcacccagca ggcggctgac
     2101 cttcccggca acgattaaaa tggcataaca aatcgcactg accaccaaac cggccgcgat
     2161 aatcaacgcg atgtcgccgt atgttttagc cgccgaagcg taaataatca cggtcgaaat
     2221 accgcccggg ccgatggtga tcggtatggc gatgggcacg acggcaatcg ctccggcatt
     2281 gcgggcgggg cgcacctgcc ccgtttccgg ctgcgcgccg agattctgct tggcgggatt
     2341 gtcgttgccg ttcatcatcg aaatggcaat cagcaacacc aaaattccgc cgccgacctg
     2401 aaacgaaccg acgctgatgc ccaaaacctt cagcagcgta ccgccgatca gcgcaaatac
     2461 cgcaatcacg gcaaacacgg caacggcggc cgtccgcgcg accttcctgc gctccttcgt
     2521 gctgtgcccg ttggtcaggt caaggtaaag cgacaacgcg ctaaacggat taatcagcac
     2581 caaaaaagcc acaatcagct tgccgatttc catgcccaat cccattattt ccccctcctt
     2641 caaacccgtg cggcaggcat ccgatgctgc aaattgccgc cgcaacggat ttttccgtta
     2701 taattaaaaa ttcaagcaat acgccccatc atacccgaac gacggtatct ttaccatcag
     2761 acaaggatgc ttttcatggc actgacactt gccgacgtag acaaaatcgc ccgactctcc
     2821 cgactgcacc tgactgcggc agaaaaagaa aaatcgcttc aagaattaaa cgacattttc
     2881 gctatggtcg aacagatgca gaacatcaac acagacggca tcgaaccgat ggcgcatccg
     2941 cacgaggccg ccctgcgcct gcgcgaagac gaagtaaccg aaaccgaccg cgccgccgaa
     3001 tatcaggcgg ttgctccgga agtacgcaac cgtctgtaca tcgtaccgca agttatcgaa
     3061 gaataatccg aatacgcttc agacggcatc agcaataccg cccgaagccc tttaaggatg
     3121 gaagatttat gacccaatac acattgaaac aggcaagcgt cctgttgcag tccaaacaga
     3181 tttccgccgt cgaactggca agcgcatacc ttgccgccat cgccgaaaaa aatcccgccc
     3241 tcaacggcta tatcaccatc gaccaagata aaacccttgc agaagcccgt gccgccgacg
     3301 aacgcatcgc tcagggcaac gcttcggcac ttaccggcat acccgtcgcc tacaaggata
     3361 ttttctgcca aaccggctgg cgcagcgcgt gcgcttccaa aatgctcgac aacttcatct
     3421 ccccctacac cgccaccgtc gtccaaaaac tgctcgacga aggcatggta acgctcggcc
     3481 gcaccaatat ggacgagttc gctatgggtt cgaccaatga aaactcattc tacggcgcag
     3541 ccaaaaaccc gtggaatccg gaacacgtcc ccggcggttc atcaggcggt tcggcagccg
     3601 tcgttgccgc gcgcctcgcc cctgccgcgc tcggttcgga caccggcggc tctatccgcc
     3661 aacccgcatc gcactgcggt atcaccggta tcaaacctac ctacggcacg gtttcccgct
     3721 tcggtatggt cgcctacgcc tccagcttcg atcaagccgg cccgatggcg caaactgccg
     3781 aagactgcgc gattctgtta aacgcgatgg caggtttcga cccgaaagac tccaccagcc
     3841 tcgagcgcga aaaagaagac tacacccgcg atttgaacca accgctcaaa ggtttgaaaa
     3901 tcggcctgcc caaagaatat ttcggcgaag gcaacagcgc cgatgttctg acggcattgc
     3961 aaaacaccat tgatttgctg aaagcccaag gcgcggaatt gattgaagtt tccctgccgc
     4021 aaaccaagct gtccattccg gcctactatg tcctcgcctc cgcagaagcc agtaccaacc
     4081 tttcacgtta cgacggcgta cgttacggac accgtgccgc ccaattcagc gatttggaag
     4141 aaatgtacgg caaaacccgc gccgaaggtt ttggcagcga agtcaaacgc cgcatcatga
     4201 tcggcactta tgtactgtcg cacggctact acgatgccta ctatctcaaa gcccaaaaac
     4261 tgcgccgcct cgttgccgat gattttcaga cggcatttgc acggtgcgac ctcatcctcg
     4321 cgccgaccgc accctccgcc gcacccaaaa tcggagcgga tgcttcgccg gttgaaacct
     4381 acttgagcga tatctacacc atcgccgtca acctcgccgg actgcccgca ttgaccctgc
     4441 ccgcaggttt cagcagcggc ggactgccca tcggtgtaca gcttatcggc aactatttct
     4501 ccgaagccaa aatcctcggt gcggcgcatc aaatccaact caacagcgat tggcacggca
     4561 aacgacccga atgaagcaga accgcacctt taccttcccc gattttcgca ccgtttacag
     4621 ctatgcgcct ttatatcggc tgcaacattt aaaatacaca ttgcgaaaat ttttcggaaa
     4681 aaaagaaatt tacgccttcg agcagtttgt caacgcctcc cctatccgtc aggggctgtt
     4741 cctccactgc ccgcaaaatg cctatccgct gctgcgcgaa tttgttgaca ggcgttttaa
     4801 ctgcaaacgc cgtttagatg cgatgacggc agattttctt atggcggaaa aactgttcgg
     4861 cacagacatt ctgcaccaaa tggaagacta ccgcttccat ttggtcttgg cgcacctttc
     4921 agacggcatc agcttgtggc tcaaccgcaa cgacaactgc gtcgaagaag gcgcgtggtc
     4981 tttatctttg cgcgacgaag caggcaaccg gctgtatatg gcgaccttcg cctttgtcgg
     5041 cacacacctg ctgacagcct ccgtacaagg gccggcgggt gaagaagcca aagacaccgt
     5101 ccgccgcata accaaacaac tccacggctt gcgtccccaa caactgatgg taaccgccct
     5161 gcaatatttc gccgccgtac tcggcttgga cggcgcaatg ggcattgcac aaaaacatca
     5221 ggtcaaactg cgctggaaac ttaaaaagcg tgtcaaaatg aattacgacg cattctggca
     5281 ggaatacggc gcaagtttgg aacgggacgg ctactggcat ctcccccaaa cccccgcccg
     5341 caaagacctt accgacatcg aaagcaaaaa acgctcgatg taccgcaagc gttatgaaat
     5401 gctggacaac atggttgcag agatgaagga cggtctgaaa acagaagcac gcggcatttc
     5461 agacggcatc caaacggaaa aaccgctccg ccggacagcc tgacgcgaag actatcgaat
     5521 tgatatttta gagaaagaag ctcttatgac ctgggaaacc gtaatcggct tggaaatcca
     5581 cgtccaattg aacaccaaat ccaaaatctt cagcggcgca tcgaccgcat tcggcgcaga
     5641 acccaacgcg cacgccggcg tggtggaatg tgctttgccg ggcgtactgc cggtaatgaa
     5701 ccgagaagtc gttgaaaaag ccatcaaatt gggtttggct ttggatgcga aaatcaaccg
     5761 gaaaaacgtg ttcgaccgta aaaactactt ctatcccgac ttaccaaaag gctatcaaat
     5821 cagccagttg gacttaccga ttgtcgaaca cggcaaattg gaaatcgtag tcggcgacga
     5881 tgtgaaaacc atcaacgtaa cccgcgccca tatggaagaa gacgcgggca agtccgtaca
     5941 tgaaggcttg aacggcgcga ccggtatcga tttgaaccgc gccggcacgc ctttattgga
     6001 agtcgtatcc gaacccgaaa tgcgctccgc cgccgaagcc gttgcctacg ccaaagcctt
     6061 gcacagcttg gtaacctggc tgaacatttg cgacggcaat atggcggaag gttcgttccg
     6121 cgtcgatgcc aacgtatccg ttcgcccgaa aggtcaagcg gaattcggca cgcgccgcga
     6181 gattaaaaac ctcaattcct tccgtttctt ggagcaggcg attaattacg aagcagaagc
     6241 gcaaatcgag attttggaag acggcggcaa agtacagcag gcaacgatgc tgtttgatcc
     6301 cgaaaaaggc gaaacccgcg taatgcgcct gaaagaagac gcgcacgact accgctactt
     6361 ccctgacccc gatttgctgc ccgtcatcat ttcagacgac cggttgcaaa aagcaaaagc
     6421 agaaatgccc gagctgccaa aagaaatggc ggcgcgtttc gtggcggatt atggcgtgag
     6481 cgactacgac gcgcgcctgc tcactgccag ccgcgtgcag gctgcctatt ttgaagaagc
     6541 cgctaaagag agcggacaag gcaagctgac tgccaactgg atgaacggcg aacttgccgc
     6601 cacgctgaac aaagaaggca tggaacttgc cgacagcccg attaccgccc cacgcctcgc
     6661 cgctctggta ggcaaaatcg ccgacggcac attaagcagc aaattggcga aaaaagcctt
     6721 tgaagccatg tgggctgaac ccgaagcctc cattgccgaa atcattgaaa aacacggttt
     6781 gcaacagatg accgataccg gcgcgattga agcgatggta gacgaagtgc tggcaaacaa
     6841 tgcaaaagcc gtggaacagt tcaaatccgg caacgaaaaa gccctgaatg cgattgtggg
     6901 gcaagtgatg aaggccagca aaggcaaagc caatcccgcg caggttcaag agctgattaa
     6961 agccaaactt gcctgataag caagatgccg tctgaaaccc aaatccgacg ttcagacggc
     7021 attttcacag tctgaccaca ttaaaacagc ataaaaaaca atattccatg aaccacacca
     7081 ttacactgcc cgaccaaacc acctttgccg ccggcgacgg tgaaaccgtt ttagccgccg
     7141 ccgcccgtca aaacctcaat ctgccccatt cctgcaaaag cggtgtctgc ggacaatgca
     7201 aagccgaact ggtcagcggc gatattcaaa tgggcagaca ctcggaacag gctttatccg
     7261 aagcagaaaa agcgcaaggc aagattttga tgtgctgcac cactgcgcaa agcgatatca
     7321 gcatcaacat ccccggctac aaagccgatg ccctacccgt ccgcactctg cccgcacgca
     7381 tcgaaaatat tattttcaaa cacgatgtcg ccctcctgaa acttgccctg cccaaagccc
     7441 cgccgtttgc cttctacgct gggcaataca ttgatttact gctgccaggc aacgtcagcc
     7501 gcagctactc catcgccaat ccgcccgacc aagaaggcat tttggaactg cacatccgca
     7561 ggcgtgaaaa cggtgtctgc tcggaaatga ttttcggcag cgaacccaaa gtcaaagaaa
     7621 aaggcattgt ccgcgttaaa ggcccgctcg gttcatttac cctgcaggaa gacagcggca
     7681 agcccgtcat cctgctggca accggcacag gctacgcccc catccgcagc atcctgctcg
     7741 accttatccg ccaaggcagc agccgcgtcg tccatttcta ctggggcgcg cgtcatcaag
     7801 atgatttgta tgccctcgaa gaagcacaag ggttggcaga ccgtctgaaa aacgcctgct
     7861 tcacccccgt attgtcccgc cccagagagt gctggcaagg aagaaaaggc cacgtacaag
     7921 acatcgcggc acaagaccac cccgacctgt cggaatacga agtatttgcc tgcggctctc
     7981 cggctatgac cgaacaggca aaaaatctgt ttgtgcaaca gcataagctg ccggaaaact
     8041 tgtttttctc cgacgcattc acgccgtccg catcataatt ccccggtata aaaaagattc
     8101 aggctttccg ttcggaacac aaaaaacttc ccgtccgtat tttccccgtg aaaaaatgcc
     8161 gtctgaaacc cgattcctgt tttcagacgg catatgtttt ttcctgttca aggcgacagc
     8221 cgctcgcgta tccagccgcc atccagcaaa cggtattgga tgcggtcgtg cagcctgctc
     8281 ggcctgccct gccaaaactc aatcaaatcg ggaatcacaa tatagccgcc ccaatgcggc
     8341 ggacgcggca cgtgcaaagg atgtttgagt ccgaccgccg ccgcctttgc caccaatacc
     8401 gccttgttcg gaatcacttc gctctgcgcg cttgcccacg cacccaaacg gctctgatac
     8461 gggcggctct caaaatattc gtccgacaat ttctccgcca gcctttcaac acgcccttcc
     8521 acgcgcacct gacgctccag ctccggccaa aaaaacgtca tcgccgcaaa tggatgagca
     8581 tccagcgaac gccccttgcg gctgtgataa ttcgtaaaga aaacaaaacc ttcagaatta
     8641 acttccttca gcagcaccat acggctgttg ggtctgccgc gtccgtcgac cgccgccaca
     8701 ttaaccgccg tcggctcgtt aacctgtgcg cgtaccgcct cgtccaacca ccgctcgaac
     8761 tgctcgatcg gattgtcggc gcaatcgcct tccgacaatt cccgtttgct gtaatcttcc
     8821 cgaatattgt gcaaatccat ttaccgctcc tcttgttatt tgaaagattc tacccccgca
     8881 cgcaaaccga tttcaaccgt tgcacaaact ttgtccccaa agccgcagcg acgatttcat
     8941 ccgcaaaacc gccgcatcag gtacaatatc gaaccgtccg actgaggacg gcattttatc
     9001 aacccgtcct gccgcatacg ccgcagaaga accgctttat caagcgagtt aggaaaaatg
     9061 atgtccaaac agcccaccag caaacgccaa tggcgcgacg gcgcagcccc gtctgccaag
     9121 aaaaccgcca aaccgttcaa aagcaaagcc cgtcccaaag acgaaacgga caaaaccgca
     9181 tcccaacctt acggacaaaa agcttcagac ggcatcaaac ctcaaaacct ccccaaacag
     9241 cgcgccgcca aagccaaaaa actcgtcgtc cgcaatccca accaaaaaat tatggaacac
     9301 gcgcgcgatt taaaagaacg ccgcagcgac ctatcgcgca tggaacccga acgcctgcaa
     9361 aaagtgcttg ccgcgtccgg cgtcggctcg cgccgcgaaa tggaagaatg gatcaacaac
     9421 ggctgggtaa cggtcaacgg caaaaccgct caactgggcg acaaagtcac ccccgacgac
     9481 cacgttaccg tcaaaggcag catcatcaaa ctcaaatggg ccgaccgcct gccgcgcatc
     9541 atcctatatt acaaacaaga aggcgaaatc gtttcccgcg acgacccgca aggccgcgtc
     9601 agcatattcg accgcctgcc gcaggccgcc agcagccgct gggtcgccat cggacgcttg
     9661 gacatcaaca ccagcggact tctgattctc accacctccg gcgaactcgt ccaacgtttc
     9721 gcccacccca gcttcgaagt cgaacgcgaa tacgccgtgc gcgtactggg cgggctgacc
     9781 ggcgaacaaa tgcgcgtcct caccgaagaa ggcgtgatgc tcgaagacgg cttggcaaaa
     9841 gtcgaacgca tccgcgaaca aggcggcgaa ggcgcgaaca aatggtacaa cgtcgtgatt
     9901 aaagaaggcc gcaaccgcga agtgcgccgc atttttgaaa gccaaggact caccgtcagc
     9961 cgcctcgtgc gcgtcggctt cggccccatc ggactgccca accgcctcaa acgcgggcag
    10021 ttctacgaac tcaaccccgc cgaagtcgcc aacatcctca aatgggcgga aatgctgctg
    10081 ccgggcgaat accgccgcaa aaaagcctaa acccgccaaa acacaaaaat gccgtctgaa
    10141 acatctgctg tttcagacgg cattttgttc gggcgttttc aggagaaaag gtcgagtgct
    10201 ttgacaaaga tcatcaccac gccgtaggcg agcggtatga cggccaatgc ccaacgccac
    10261 cagaccgata tgccgccgcc ggaaactttt tcgcccacaa ggtaagcgtc ggatatggcg
    10321 gtttcgtcat cggggttgcc gctgtgtgcg gcggttttga tgtctttttc gtggtgtttt
    10381 tcgtgtacgg atttgacggc gaggttgcac aacaaaccga taatcagcag acccgccatg
    10441 atgtacatgg ttacgctgta tgcctgtgcc gccggtatgc cgctgtcgat ttggctttgg
    10501 cgtatgtaat tgaccagtac cgggccgatg acggcggcgg ttgaccaggc cagcaggatg
    10561 cgtccgtgaa tcgcgccgac ctgataggtg ccgaacaggt ctttcaggta ggcgggaatg
    10621 gcggcaaatc cgccgccgta catggaaata atcacgcaaa agccgatgat gaacagggct
    10681 ttgctgccgc cctcgccgat ggagggaacg gcgaaataca gcagcgaacc gagtacgaag
    10741 aagatggtgt aggtgttttt gcgtccgatt ttgtcggaaa cgctcgacca caaaaagcgt
    10801 ccgcccatgt taaacaggct caggaggctg acgaagcctg ccgccgcacc tgcgccgact
    10861 gccgcctgcc tgcctatgga ggtttcggaa aagagttcct gaatcatcac ggatgcctga
    10921 cccaatacgc cgatgccggc agttacgttc aggcacaata cccagaacaa cagccaaaac
    10981 tgcggcgttt tcatggcttg ggacacgttg acatgattgc tgctgaccag cttgttttgc
    11041 gttttcggcg cggtatagcc ttcaggtttc cagccgtcgg caggtacgcg gatggtaaac
    11101 gcgccgaaca tcatcagtgc gaggtaaagc agacccaata cggcgaaggt ttcggcaacc
    11161 ccaaccgaag tagcgtttga aaaggtgttc atcagtgata cggaaagcgg cgaggccagc
    11221 attgcgccgc caccgaaacc cataatcgcc aaaccggtcg ccatacccgg cttgtcggga
    11281 aaccatttca tcagtgtgga aaccggcccg atgtagccca aacccaagcc tacgccgccg
    11341 atgacgccgt tgcccaaata gagcaggaag aggttgtgcg tacgcacgcc gaatgcggat
    11401 acgaagaagc ccaggctgaa acagcaggcg gcggcaaata tggctttgcg cggccctacc
    11461 cgttccatcc acgtaccgaa cagggcggcc gacgcgccca gcatcgcgag tgcgatactg
    11521 aaaatccaac ctacggtcgt cagcttccaa tctccggccg ccgattcggt tatgccgata
    11581 agtttggtca gcggcgcgtt gaatacggaa taggcgtaaa tctgcccgat ggcaaggtgt
    11641 accgccaatg ctgcgggcgg tacgagccaa cggttgaaac ccggcttggc aatgcttgcc
    11701 tcacggtcta aaaacttcat aacatcctct ttctgtcagt tgaaaaataa aatttcattt
    11761 gctcaatgga aacttattga aaattataaa aaaatatcgg gtcgggtttt tatccgcccc
    11821 aagatgcgcc gtctgaaaca tttcgggtgt acggaaaggt ttctgttttt tccgacaaat
    11881 tcctgcggct tttcgcttcc ggattcccgc ttttgcggga atgacgaatt aaagattatc
    11941 ttaaggtcaa gggactggat tcccgcttt
//
LOCUS       DAWKQB010000053        11772 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.053, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000053 DAWKQB010000000
VERSION     DAWKQB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.053"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            96..1280
                     /gene="coaBC"
                     /locus_tag="WJL14_001079"
     CDS             96..1280
                     /gene="coaBC"
                     /locus_tag="WJL14_001079"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ7656158.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGSNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTELPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
     gene            complement(1347..3503)
                     /locus_tag="WJL14_001080"
     CDS             complement(1347..3503)
                     /locus_tag="WJL14_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7656159.1"
                     /translation="MPAPQPSAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEYAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3595..3801)
                     /locus_tag="WJL14_001081"
     CDS             complement(3595..3801)
                     /locus_tag="WJL14_001081"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ7656160.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3860..4477)
                     /gene="gmk"
                     /locus_tag="WJL14_001082"
     CDS             complement(3860..4477)
                     /gene="gmk"
                     /locus_tag="WJL14_001082"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ7656161.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEANGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4605..5204
                     /locus_tag="WJL14_001083"
     CDS             4605..5204
                     /locus_tag="WJL14_001083"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ7656162.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5250..6038)
                     /locus_tag="WJL14_001084"
     CDS             complement(5250..6038)
                     /locus_tag="WJL14_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ7656163.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVCEVPMDAGLMGRVCAHLDSL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMCNGEQAAKEVAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6363..7718
                     /gene="yegQ"
                     /locus_tag="WJL14_001085"
     CDS             6363..7718
                     /gene="yegQ"
                     /locus_tag="WJL14_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ7656164.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATENDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7852..8328
                     /locus_tag="WJL14_001086"
     CDS             7852..8328
                     /locus_tag="WJL14_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ7656165.1"
                     /translation="MLAVCQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKFDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8536..10909)
                     /gene="hmbR"
                     /locus_tag="WJL14_001087"
                     /pseudo
     CDS             complement(8536..10909)
                     /gene="hmbR"
                     /locus_tag="WJL14_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
     gene            complement(11084..11713)
                     /locus_tag="WJL14_001088"
     CDS             complement(11084..11713)
                     /locus_tag="WJL14_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ7656166.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     AGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
BASE COUNT         2849 a         3154 c         2878 g         2891 t
ORIGIN      
        1 ctcagacggc attttgtatc caaacggtaa ctaatgtatc cgcactttgt tatagaatgg
       61 ctgctgtttt ttcttcgtaa ttagaaattg tcaaaatggg taaacatatt cttttaggtg
      121 taacgggcag tattgcggcg tataagtctt gcgagttggt gcgactgctg aaaaaacagg
      181 ggcattcggt tacggtggtt atgagccgct cggcaactga atttgtttct ccgctgactt
      241 ttcaggcttt gagcggcaat cccgttctga ccgacacgca cggcagcaac ggttcaaacg
      301 gcatggaaca tatcaacctg acccggaatg cggatgtttt tctgattgcg ccggcaagta
      361 tgaataccgt ggcaaaaatc tgtaacggcg tggcagataa tctactgacc aatctggcag
      421 ccgcacggaa atgtcctctt gccatcgctc ccgcgatgaa tgtggaaatg tggctcaacc
      481 ctgccaacca acggaatatc gcacaactgg tttcagacgg cattactgtc tatatgccgg
      541 gcttgggcga acaggcttgc ggagaaaatg gcatgggaag gatgccggaa cctgccgaat
      601 tgctggattt gcttccggac ttgtggacac cgaaaatttt aaggggcaag aaaatcttga
      661 ttaccgcagg tgcgacattt gaagccattg accctgtcag gggcatcaca aatacctcca
      721 gcggaaaaat gggggtagct ttggcgcggg cgtgccgtgc cgccggtgcg gaagtcagcc
      781 tgattcacgg acagcttcaa accgaactgc ctttcggcat atccgatacg gttcaagccg
      841 tcagcgctga agatatgcat cgcgcagtgc atcgtttgat tgaaaaacaa gatgctttta
      901 tttctgttgc cgccgtctca gactataagg ttaaaaacag gagtacgcaa aaattcaaaa
      961 aagataaaaa tgccaaaccg ttatccatcg aattggatga gaaccccgat attttggctt
     1021 ctattgcctc attgccgaac ccgccgttct gcatcggttt tgccgctgaa acggagaatg
     1081 taatggcata tgcacgagaa aaacgtatta agaaaaaaat accggtaata gttgccaatg
     1141 atgtttcaat cgcgatgggc aaaccgacca accggattac cattatcggg gacgacgggg
     1201 aactgtcttt tcccgaaaca agtaaagatg aagcggcgat gcagattgtt gaaaggcttg
     1261 ccggatattt gaacaaataa gcaattgaac ggataaacca taaagcgggt tgcctgttaa
     1321 tcaaaaggca acccgtttta cctgcttcaa cttctgatga ctttgcggat atatggaata
     1381 ctatgcagat tttgaataat ctgattcaat tgattcagat tcttgacttt caataagaat
     1441 ttgaattcga caaaaccttc cgttcccgac tgggatttag acggtgtttc gaccgactca
     1501 atgtccgcac cggaatcgga aatcgcttgc gccattaatg ccagcaagcc gtggctgtct
     1561 tccgattgga cttgaagccc gacacggtag ttctgcccgt tcatattttc ccagtctgca
     1621 tccagctgct gttcgggatc ggacttcaac aacgtcgggc aggtatccct atggataatc
     1681 atgccttttc ccttaaccaa cagcaaacgg atggaatcac cgggaacagg gtggcagcac
     1741 tctgcaaaat gaatatgccc gctttcctga ccatcgactt taatggaact gagcctgacc
     1801 tcgctaccga aatgctcccc tgccaactcg gcaatgtgca tggcgacata aacaggcagg
     1861 gtatgcccca taccgacgtt gtacagcact tcctcaaatg aagtctgttt gtcgttgaga
     1921 tcggcaaggt atttttcttt gatgccgtct gaaagcagga catctttggg cagcaaactg
     1981 gacagggctt tttgtaagag gctctctccc aaaacgaccg catcgtgccg gttaaggttt
     2041 ttaatatatt ggcgtatggc actgcgcgcc ctgccggaca cggcgaaatt caaccacgcg
     2101 ggattgggtt tggcgtattc ggatgtgata atttcaacag aatcaccggt tttgagcttc
     2161 gtacgcaacg gcatcatgat attgttgata cgtgcggcaa cggttttgtg cccgatatcg
     2221 gtatgcaccg cataagcaaa atcgacaggc gttgcccctt tgggcaaagt taggattttt
     2281 ccttttggcg taaggatgta gatttcgttc ggaaacaaat cgactttgac gtgttcgaga
     2341 aactcaatgg cattggcact gcttgcctgc aaatctaaga tatttttcag ccaccggttt
     2401 gtgtgaagca ccgcctgatc gaccgtctta gaatatgatt tatagctcca atgtccggcg
     2461 attccacctt cggcaacagc atccatttcc ttggtacgta tctgaacttc aatcggcaag
     2521 ccgtaaggac cgaccaaagt cgtatgcaga ctttgatacc cgttgctttt cggaatggcg
     2581 atatagtctt tgaaccgccc gggcttaggc tgatagaggg tgtgcaatgc gccgagtgcg
     2641 gcataacagg ctggaatgct gttgacaatg acgcggaaac cgtaaatatc cataacctcg
     2701 gcaaagcgca gctttttcgc catcattttc tgatggatgc cgtacaggtt tttttccctg
     2761 cctttgattt tggcctctat attcgcgcct accagccgct ggccgaatgc gcgcaagact
     2821 ttgccgacaa cgtcctgccg gttcttccgg ctcttgtcca tcgctttttt taaagtctcg
     2881 tagcggttgg gatgcaggtt ttggaacgat aaatcctgaa gctcttgata tgcgttattc
     2941 aaacctatgc ggttggcaat ctgtgcatag atttcaaggg tttcccttgc aatccggcgg
     3001 cgtttgtccg ggcgcatcga accgagtgtc cgcatattat gcagacggtc ggcaagtttg
     3061 acgacgatga cgcgtacatc tttggtcatt gccaaaatca gtttgcggaa actctccgcc
     3121 tgatgctctg catgatcttc aaatttgagt ttttcgagtt tggacagccc gtccaccatc
     3181 tcggcaaccg tattgccgaa caccgccgcc atttcccctt ttgtcacgcc cgtatcttcc
     3241 aatacgtcgt gcatcacgcc tgcacaaaga ccctgtatgt ccatatgcca aagggcgagc
     3301 tgcgtcgcaa cggcaatcgg atgcgtgatg tagggctccc cgcttttgcg ggtttgcccg
     3361 tcgtgggcgc gaaacgcata ggcgacagct ttttcaagct ccgcctgttc ctcgggcttg
     3421 aggtaggagg cggtatggaa aagcagggca cgcgcttcgg cggtcagggg gtcgtaaggg
     3481 gcggaaggtt ggggggcggg catttcagac ggctttcggt atgtatgtgt ttttcatttc
     3541 aaaccgtcgg actgcacggc ggcaaagtgt tccggcgtgc gtttccggca gaatttattt
     3601 attgcgcgtc aacagttctg taccgatatg tccggcggcg atttccctta aggcggtaac
     3661 ggtcggtttg ttattgcgga catcgtccac aagcggcgtg ttgccgttct caagctggcg
     3721 ggcgcggcga gccgctacca atgtcaggtc aaaatggttg gaaatttttc cggtacagtc
     3781 ttcggtggta atacgtgcca tattatttgc tttctttcaa aaatatttaa attgggaaac
     3841 cgggtatttt cgccgttttc taggaatttt ccaacaaatc tgcaataaac cccagttgcc
     3901 gcgacctttt cagacggcag gcattcacaa tatggcgcaa atcctcctcc gctcgcgcca
     3961 agtcgtcatt gaccacgaca aagtcgaaca atacggactg ctcgatttca tgccttgcct
     4021 tcgacagcct cctttggata acttcccgac tgtccgtccc acgtccgttg agacgtgcag
     4081 caagcacgtc gaaagaaggc ggcaggataa agatgccgac ggcttcaggc agcgcgttgc
     4141 gaacctgcgc cgcgccctga acgtcgattt ccaaaattac gtcatagcct gccgccgcca
     4201 acgcattcac gccctccgcg cttgtgccgt aatagttgcc gaatacgtct gcgtattcca
     4261 aaaaagcctc ctgcgcgata agcgactcaa actcttcttt ggaaacaaag tgataatgca
     4321 cgccgtttgc ttcgccttca cgcggcgggc gcgtcgtgtg cgacacagaa acgcgcaaac
     4381 cgttatggtt tgccaacagc cgagaaacca gcgtggtttt gcccgtgcca gaagcggccg
     4441 aaatgataaa gatgttgcct tttcgataag cggacatatt ttttacctgt atattttcca
     4501 gccgattgta tcacaatgga cacccagttt cctatttgcc gatgcccata ttttgccgct
     4561 attgttttga ttgatttggc aagcggcagg ctgacggcta caatatggcg ttaaaaacat
     4621 caaacttgga acacgcaatg ctggttcatc ccgaagctat gagtgtcggc gcgcttgccg
     4681 acaaaatccg caaaatcgaa aactggccgc aaaaaggcat cttattccac gacatcacgc
     4741 ccgtcctgca aagcgcggaa tacttccgac ttttggttga tttattggtt taccgctata
     4801 tggatcagaa aatcgacatc gttgccggtt tggacgcgcg cggcttcatt atcggcgcgg
     4861 cactcgccta ccatctcaac gtcggttttg tccccatccg caaaaaaggc aagctgcctt
     4921 ttgaaaccgt atcgcaaagc tacgcgctcg aatacgggga agctgcggtg gaaatccaca
     4981 ccgatgccgt caaacccggt tcgcgcgtgc tgctggtcga tgatttggtt gccacgggcg
     5041 gcacaatgct tgccgggctg gaactgatcc gcaaactcgg cggagaaatt gtcgaagccg
     5101 ccgccatttt ggaatttacc gaccttcaag gcggcaagaa tatccgtgca agcggcgcgc
     5161 ccttatttac cctgcttcaa aacgaaggct gtatgaaggg ctgaaaaccg accctgccgt
     5221 ctgaaaccgg cagggttgtt attatgcatt caaatcacgc ccaaatcttg caaacccctc
     5281 aacacgccgt cttcatcaac gctggggcaa acatatttcg ccacttcttt cgccgcctgt
     5341 tccccgttgc acattgccac accgaacccg acttctgaca gcatttccac atcgttcaaa
     5401 ccgtcgccga acgccatcac gtccgccatt tccaaaccca atgcttcaac cacgcttctg
     5461 atgccgtctg ttttcgacgc ccccgcaggc agcagatcga ccgcttcctc gtgccagcgc
     5521 accgttttca agccttcccg ttccacaata tccgaccaga gcggcatttc gttttcctcc
     5581 gcaaacacca gcatctgata caccggtttg cttgaaaaat aatccttatc ggcaaaaaaa
     5641 tcgctggcga tatgcttcaa ggcgcggcac acgcattccg acagtgcgga caccgcaatc
     5701 ccctctccgc cgacaaacgc ataatccata cccaagctat ccaaatgcgc gcaaaccctg
     5761 cccatcaaac cggcatccat cggtacttcg cacacagttt ttccgtgtag cagcgcaaac
     5821 tgtccgttta tcgttaccac ggcatccatt cccgtttccg ccatcatgtc cctgaccttt
     5881 tcgggaatcg tcgccaaaga ccgccccgtt gccaacgccg tcaatatacc tttgccgcgc
     5941 aaagccgcca ccgccgtttt cacggaaggg cgcaaggtat ccgtatattt gcggtacagc
     6001 gtatcgtcaa tatcgaaaaa cacgattttg ggattcatca cattctctct cgcattcaaa
     6061 ttgccgcatt gtatcccaag caggcaaata cttgataaat ccttataaat ttcccggcaa
     6121 aattgaccgc gaacacgaaa aggcggataa tccgcccatc ctcaaaccct tttcagacgg
     6181 catttgcagc aatgccgtct gaaacatttt tacaaagcac acaaatcatg tttcaacaca
     6241 caggacgaca cataaagcgt cgccctatat gttgccctga ttcggaaagg gttacacccc
     6301 tcccaaataa agtctgattc taccgcccta aagggcgggg tttcaaccga aaaggaaaca
     6361 cgatgaaagc acccgaactc ttattgcccg ccggcggatt ggaaagaatg cgcaccgcct
     6421 acgactacgg cgcagatgcc gtttatgccg gcagcccgcg ttactcactg cgcgcccgca
     6481 acaacgaatt tgccaaactc gacgttctcg agcaaggcat taaagaagcg cacgagcgca
     6541 acaaaaaatt ctttttgacc gtcaacaccc tgccgcacaa ctccaaactc aaaaccttcg
     6601 tttccgatat ggagccgctg attgccatga aacccgacgc gctgattatg gcggatccgg
     6661 gtttgattat gaccgtgcgc gaaaaatggc cggaaatgcc gatccacttg tccgtacagg
     6721 ccaacaccac caactactgg ggcgtgaaat tctggcagaa tatcggcgtc gaacgcatca
     6781 tcctgtcgcg cgaattgagt atggaagaaa tcgccgaaat ccgccaacaa tgccccgaca
     6841 tcgaactcga agtcttcatt cacggcgcat tgtgcatcgc ctactccggc cgttgcctac
     6901 tctccggcta cttcaaccac cgcgacccca accaaggcac ctgcaccaac tcctgccgtt
     6961 gggactacaa agtccacaac gctacggaaa acgatgcggg cgatgcccag cttctgcaag
     7021 gttttaactt tgaaaaagcc caagaagaag ccaaccaaaa ctttgaaggc atcaacggtc
     7081 aaaaacgcca tccctacgcc gacaaagttt tcctgattga agagtccaac cgcccgggcg
     7141 agatgatgcc gattatggaa gacgaacacg gcacttacat catgaactcc aaagacctgc
     7201 gcggcattga agtggttgag aaactcgcca aaatcggcgt ggacagcctc aaagtcgaag
     7261 gccgcaccaa gtcgctctat tatgtcgcac gcgtcgccca atcctaccgc aaagcgattg
     7321 acgatgccgt cgcaggccgt ccgtttgatt acagcctgtt gagcgaactc gaaggccttg
     7381 ccaaccgcgg ctacaccagc ggcttcctcg aacgccacca aacccaagac tatcaaaact
     7441 acctcagcgg ccattccacc gccaaacaaa gccaatacgt cggacacgtt accgaaatcg
     7501 acaaaaacgg ctgggcgacc atcgaagtta aaaaccgctt cgccgtcggc gattcactcg
     7561 aaatcatcca cccgaacggc aaccaaacca tcaaattgga acaaatggcc cgaaaaggcc
     7621 agcctgtcga tgtcgcaccc ggcaacggca ttcaggtcaa aatccccgat atgcagggca
     7681 aagaaaaagc cctcatcgca cgcgtgttga acccctgagc cacaatgccg tctgaacccc
     7741 gttttcagac ggcattttgt tgaatcttac ggtacaatgg caatgatttc ccaccgcaag
     7801 caaggatatc cgacatgaaa aaaaatcctc atactctccg ccgccgccct gatgctggca
     7861 gtctgccaat ccggcagcgc acctaaaaac gcgcacacgg catcccccgg cacaccggcc
     7921 ggagaatcat gcaaagtcat ccggggcgac accgcaggaa gcggcaaaaa catcatttac
     7981 cgttgctgca acggtcaggc ggcaataaat gcagccattg cagacggcgt attggaatca
     8041 gggattcccg tcagcttcgg cggcgggggc gtaccggttg caaacggcag gaatctagtt
     8101 acccgtcagg cggcaaacgg cataggcaaa ttcgactcag aagcctacga acgcgcccta
     8161 atcaatgccg cccgcaaatt ccaacagacc gcaggcaaac tcggcggacg cggcgttacc
     8221 ggcttccaca gctactttga caagcaaccg ctgcaaggcg ggcaatacga ctgtcaggca
     8281 ggctcgttcc acgtccgcgt ggtcatgcgc gccaatgtcg tccgttaaca tatcggcaat
     8341 caaaaaatgc cgtctgaaac atttttcaga cggcattttt aatcctgcaa cattactccc
     8401 tgcctgagtt cggatactgt atcaatataa aaccccatca cacagattta cggtaaaaag
     8461 ccgtccgaat gaattcttga acacaattcg gacggcttta attttcaaca aggcgattaa
     8521 ttcaataata ccagattaaa acttccattc cagcgatacg gcgtaattgc ggcctggggc
     8581 gcggtagcgg tctaagcctt tgccatcgcg gtcgaccgca ttggtggtgc tgtagctata
     8641 taaaccgcgc agggaatccc aagtggtgta tttgcggttg aacaggttgt acacgcccgc
     8701 acgcagggtc aggtttttca ccggtttgta gaagccgtac atatcgaaca cataagccga
     8761 cttgttcagc cacgggtaat cttttacctt tttctgcaaa ggcgtacccc agcccttgtt
     8821 ttcataaacg gtgtattgcg cgtctttgac ctttttcgcg cctagatagg tcaggcggga
     8881 gaatacgccc catttttcgc tcggactttc atagtcgata ccggcaatca ctttcagcgg
     8941 ctgtgtggac agcaggctgt tgtcgcccga cagtttgctt ttcgcataac ccagcgagcc
     9001 gaacagtttc cagccctcag gaacaaaaga cgctactttg tccacattca gacggcctgt
     9061 cagctcgata ccgcggattc tggccttgtc gatatttttc atctgccaat ccagtttttc
     9121 tttgtagggg tcgctgcata tactgtagta agcattttcc tcagtacagc cgggagtgcc
     9181 gctggtggtc agcttctgct cttcagacag gaaattgcgg taattgcttt gatacaggtt
     9241 ggcatccagc atgccttttt cgctgcggcc ttgcagagac agggtgtggg tggtgctgcg
     9301 ctcggctttc aggttgggat tgggcagcca attacccgaa ccgtggttgt aagtgaaata
     9361 cacttcggac gcattgggga cacggtagcc ggaagtaatg tcgtaaccga cacgccaagc
     9421 ctgattcagt tgcgccgcca agccgacaaa accgctccag cctttataag tgttggctgc
     9481 aggtggtgtt ttgtcacaag catgacactc ggcattcaat tcctgaggcg tcattttggt
     9541 gtggtcgtaa cggatacctg cgcggctact gaacacgtcg ttccattgaa tttggtcaga
     9601 cagtgagaaa ccgtagttgg tggttttcac cggatgctgg atactgctgg tggttcgaac
     9661 aacacggccg ctgaagtaat aatcgtcgcg gtttaggttt tcaaaatcac ggcggctggc
     9721 gaaagtttta aacgacaggc ggtgtcgccc ccccgagttg caacggatgg ctgtccaaac
     9781 gcaaagtaaa acgtttgaat cgggtgtcca tgctgcggtt gtatatttcg ccaacttcct
     9841 ttttatggta ctcagtttcc catgtggtgt aattcgtcgg gaacaggcct ttgtagttaa
     9901 tcgccgccac tttggttttc tgataatcga agtccgcctt caaagacgac aaccaatttg
     9961 aatcaggcat ccattcgtaa aagaggttgg cattgcgccg tctgtttacg tcatcggctt
    10021 cgcgccagga agaagcggtc aggttataag actcttcaac cgtgtaatta tgcccctgct
    10081 gaccgttgag cgatgcgccg atgcggtggt tgtcgttgat ttgataagca atcttaccca
    10141 agaagttgtg gtatttgtgt ttggacggat cggggatgcc gcgggcggaa ccgcggatat
    10201 tcgtttcttt acccgcacct tctaccggat agccgcggtt gcccgcgctt tcggtttcat
    10261 ggccgcgccg ttgcgaatac agcaaagcag catccacgcg gtcgttactc acaccgaaac
    10321 cgagggtatt tgtccattca cggttacgcg tgctgtaacc gtttttcatc atcacgccga
    10381 attgcctgtc gtccaacagc aaatcacggc cttgcagcgt ttggtaattc acaccgccgc
    10441 ccaatgcacc actgccggta ttgaaagagt ctgcgccctt cacgatttca atattgcgta
    10501 cgagttcggg gtcgatagac aaacgcgagc tgttgaagtt gccataacgg gcgtacagcg
    10561 agttttcttc ggaatcaggc aggtttacac cgtctatgct cacgccgaca cggttgcctt
    10621 ccacgccgcg aacagcaaag cctttttgat ggcggccgct gtcgctcaag ccgacatcgg
    10681 tggaatagcg caccaagtct ttattgtcgc gtatcatttc ttgtttgata cggttaaggt
    10741 tgacgcgttc cacagccgca ggcgcattgc gctgaccttt aacgcgcact gcttttatct
    10801 ctgccttaac gggtgtggtt tcagttgcag cttcatctgc tgccaagacc ggattgccga
    10861 aaatactgcc gaccagcgcg gcgataggga gcatttgtaa tggtttcata ttatcaactc
    10921 aagatgtatg gattgttcat ccatcggtta gcgaataata gattttatga taatcattaa
    10981 tatttaataa gacagtaatc catgtaaaca aagccgcgcc gtgtaattaa aggtccctgc
    11041 aaacagctat gccgagacct tgtttatttg gttcgattat tttttagtgc ctgtgcggca
    11101 tcattccttc cggtgcttcg gcatcggctg ccaaaccgaa ggtttcgcgc aacacgactt
    11161 tgtagaatgc aaaggcttcg cgcgcgcctt ggatggcttc cgcttcggct tcgggagtca
    11221 ggttcaaagc gttcagatgc tcgacgaagg cgcgccagtg tttgccgcgc ccgtcgggat
    11281 gcggagcgag gtggcgcgcg ccgtgttcgc cgttgtaatc gagtttttgc gcgtgtttga
    11341 acaaaaacgc cgcgcccaaa ttggatcctt cggcgcaata aagccagccg attgctttgt
    11401 tgccggcttc gtatggcaat tctttgtcga atttgtaagg ctcttcgccc aagtctttca
    11461 agtcttgcgt tacggcatcg tatcgcgcca tgtattccag ctcgggaatg gctttgttta
    11521 attcggcatc tttataaata tgatcgaccg ccttgtggaa aacggattgg agtttcaaaa
    11581 atttgatgta gttttctttg ctgacaaacg gttggacaga cataacgaga ttatccacgc
    11641 tgtcgtgaac cgccgtggta tccgccttca gacgtttggc aaacgtcagg gcttgatttt
    11701 cggtttcact cataagtttt cctttgtcgg taagggataa ggatgcgggg cgggcaaaat
    11761 ccgcacttgc cc
//
LOCUS       DAWKQB010000054        11474 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.054, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000054 DAWKQB010000000
VERSION     DAWKQB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11474
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.054"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..501
                     /locus_tag="WJL14_001089"
     CDS             <1..501
                     /locus_tag="WJL14_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HEZ7656167.1"
                     /translation="ILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLA
                     VFGDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIR
                     AFEAASGLTIPYEVKPRRAGDLACFYADPSYTKAQIGWQTQRDLAQMMEDSWRWVSNN
                     PNGYDD"
     gene            617..1642
                     /gene="rfbB"
                     /locus_tag="WJL14_001090"
     CDS             617..1642
                     /gene="rfbB"
                     /locus_tag="WJL14_001090"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ7656168.1"
                     /translation="MRKILVTGGAGFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESL
                     TDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNI
                     VGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPE
                     KPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQWYLDN
                     KTRRQNA"
     gene            1748..2614
                     /gene="rfbA"
                     /locus_tag="WJL14_001091"
     CDS             1748..2614
                     /gene="rfbA"
                     /locus_tag="WJL14_001091"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ7656169.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2668..3207
                     /gene="rfbC"
                     /locus_tag="WJL14_001092"
     CDS             2668..3207
                     /gene="rfbC"
                     /locus_tag="WJL14_001092"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ7656170.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCNRTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQGE
                     PNLSPKDLAGKVLAEAVTF"
     gene            3231..5345
                     /locus_tag="WJL14_001093"
     CDS             3231..5345
                     /locus_tag="WJL14_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ7656171.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTIPSETLAQARQAMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKSETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLVQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPKIGSLIQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     DDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLVAGTLIYYPDYIHPKTHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            5482..6741
                     /locus_tag="WJL14_001094"
     CDS             5482..6741
                     /locus_tag="WJL14_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ7656172.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNLRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWSHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKNTSDSSTPTT"
     gene            7110..8516
                     /locus_tag="WJL14_001095"
     CDS             7110..8516
                     /locus_tag="WJL14_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ7656173.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYAEAAFVLG
                     NHLLQNGQPEQALSWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAEAQACAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            8734..9948
                     /gene="gltS"
                     /locus_tag="WJL14_001096"
     CDS             8734..9948
                     /gene="gltS"
                     /locus_tag="WJL14_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ7656174.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            10047..11063
                     /locus_tag="WJL14_001097"
     CDS             10047..11063
                     /locus_tag="WJL14_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7656175.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAGEDAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            11074..11271
                     /locus_tag="WJL14_001098"
     CDS             11074..11271
                     /locus_tag="WJL14_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656176.1"
                     /translation="MNTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAV
                     RLGENGFTEEQMENIANLFGR"
     gene            11289..11438
                     /locus_tag="WJL14_001099"
     CDS             11289..11438
                     /locus_tag="WJL14_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656177.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVAYADVSTHRTIQKTS
                     PYVPS"
BASE COUNT         2909 a         3281 c         2720 g         2564 t
ORIGIN      
        1 attttgttgc gttatttcaa tccaattggc gcgcatgaaa gcggcttgat tggcgagcag
       61 ccaaacggta tcccaaataa tttgttgcct tatatctgcc aagtggcggc aggcaaactg
      121 ccgcaattag cggtattcgg cgatgactac cctacccctg atggcacggg tatgcgcgac
      181 tatattcatg tgatggactt ggctgaaggc catgtcgccg ccatgcaggc gaaaagcaat
      241 gtagcaggca cgcatttgtt gaacttaggt tccggtcgcg cttcttcggt attggaaatc
      301 atccgcgcat ttgaagcggc ttcgggtttg acgattccgt atgaagtcaa accgcgccgt
      361 gccggtgatt tggcgtgctt ctatgccgac ccttcctata caaaggcgca aatcggctgg
      421 caaacccagc gtgatttggc gcagatgatg gaagactcat ggcgttgggt cagcaacaac
      481 ccaaatggct acgacgatta agttgacctg atacaggccg tctgaaagag atgttttcag
      541 acggcctctt tatctgaaaa acacacattc tgtctgctat aatctgttta tattttttgg
      601 ctatcctctg aaatttatga gaaaaatcct tgttaccggc ggtgcgggct ttatcggctc
      661 tgccgttgtc cgtcatatta tccgaaacac ccaagattcc gtcgtcaacc tcgacaaact
      721 gacctacgcg ggcaatctcg aatcgctgac cgacattgcc gataatcccc gctatgcttt
      781 cgagcaagtg gacatttgcg accgcgccga actcgaccgc gtattcgcac aacaccggcc
      841 cgatgccgtg atgcacttgg cggcggaaag ccatgtcgac cgctctatcg gttcggcagg
      901 cgagtttatc caaaccaata tcgtcggcac attcaaccta cttgaagctg cgcgtgccta
      961 ccggcagcaa atgccgtctg aaaagcacga agccttccgt ttccaccata tttctaccga
     1021 tgaagtttat ggcgatttaa gcggcacaga cgatttgttt accgaaaccg cgccctacgc
     1081 gccgtccagc ccttactctg cctctaaagc gtccagcgac cacctcgtcc gcgcgtggtt
     1141 gcgtacttac ggcttgccga ccattgtaac caactgctcc aacaactacg gtccttacca
     1201 ttttccggaa aaactcattc ctttgatgat tctgaacgcg cttgacggca aaccgctgcc
     1261 tgtgtacggc gacggtatgc aaatccgcga ctggctgttt gtcgaagacc acgcgcgcgc
     1321 actgtatcag gttgttaccg aaggtgttgt cggcgaaacc tacaatatcg gcggccacaa
     1381 tgaaaaagcc aatattgaag tcgtcaaaac catctgcgcc ctgctggaag aactcgctcc
     1441 cgaaaaaccg gccggcgtgg cgcgttatga agatttgatt actttcgtac aagaccgccc
     1501 tggccacgat gcccgctacg ccgtcgatac ggcgaaaatc agacgggatt tgggctggca
     1561 gcctttggaa acctttgaat ccggcctccg caaaacagtg caatggtact tggacaacaa
     1621 aactcggcgg caaaacgcat aaaacggcag ctatcgtttg gaacgtttag gcaccggaaa
     1681 ataagttttc agacggcatc ccgacgcaat gccatctgaa aacccatcgc aaaggaagaa
     1741 agaaaagatg aaaggcatca tactggcagg cggcagcggc acgcgcctct accccatcac
     1801 gcgcggcgta tccaaacagc tcctgcccgt gtacgacaaa ccgatgattt attacccctt
     1861 gtcggttttg atgctggcgg gaatccgcga tattttggtg attaccgcgc ctgaagacaa
     1921 cgcctctttc aaacgcctgc ttggcgacgg cagcgatttc ggcatttcca tcagttatgc
     1981 cgtgcaaccc agtccggacg gcttggcaca ggcatttatc atcggcgaag aatttatcgg
     2041 caacgacaat gtttgcttgg ttttgggcga caatattttt tacggtcagt cgtttacgca
     2101 aacattgaaa caggcggcag cgcaaacgca cggcgcaacc gtgtttgctt atcaggtcaa
     2161 aaaccccgaa cgtttcggcg tggttgaatt taacgaaaac ttccgcgccg tttccatcga
     2221 agaaaaaccg caacggccca aatccgattg ggcggtaacc ggcttgtatt tctacgacaa
     2281 ccgcgccgtc gagttcgcca aacagctcaa accgtccgca cgcggcgaat tggaaatttc
     2341 cgacctcaac cggatgtatt tggaagacgg ctcgctctcc gttcaaatat tgggacgcgg
     2401 tttcgcgtgg ctggacaccg gtacgcacga aagcctgcac gaggcggctt cgttcgtcca
     2461 aaccgtgcaa aacatccaaa acctgcacat cgcctgcctg gaagaaatcg cttggcgcaa
     2521 cggttggctt tccgatgaaa aactggaaga attggcgcgc ccgatggcga aaaaccaata
     2581 cggccaatat ttgctgcgcc tgttgaaaaa ataatgtttg aggccgtctg aaacttttca
     2641 gacggccttt agatgaaaga taaaaagatg aacatcattg ataccgccat tcctgacgta
     2701 aaactgcttg agccccaagt cttcggcgac gcgcgcggct tttttatgga aaccttccgc
     2761 gacgaatggt tcaagaccca agtctgcaac cgcaccttcg tgcaggaaaa ccactccaaa
     2821 tccggcaaag gcgtattgcg cggcctgcac taccaaaccg aaaacacaca aggcaaactc
     2881 gtacgcgtag ttgtcggcga agtgttcgac gtggccgtcg atatgcgcga aggttcgccg
     2941 actttcggca aatgggcggg cgcaaccctg tccgcgcaaa accaatgcca gctttggata
     3001 cccgaaggct tcgcgcacgg gttttgcgtt ttgggcgacg cggcggaagt cgtgtacaaa
     3061 tgcaccgact attacaagcc tgaagccgaa caggttttaa tatggaacga cccgacagtc
     3121 ggcataggct ggccgttgca aggcgagccg aacctgtcgc ctaaagactt ggccggcaaa
     3181 gtattggctg aagcggtcac gttttaagac tattcaggcc gtctgaaaga atgttcctct
     3241 tttcagacgg cctacaatct attaataaca ataatcgacg aaaacgcatt gtgaaaaacg
     3301 cctacatccc ctctcgcggc atccgcaaaa tcccccatct ctccacccta ttgcccgagt
     3361 ttcatatcta caaagacgga aaaggagcag aggctgttgt cggctggggt ttgcgcccga
     3421 cgacacacaa agcgcgtgct tttgccaccg aacaccagct tccctttatt gctttggaag
     3481 acggcttttt acgctcgctc ggactgggcg tctccggtta tcccccctac tccatcgtct
     3541 atgacgacat cggcatctac tacgacacta cccgcccttc tcgtttggaa caactgattc
     3601 ttgccgccga taccataccg tctgaaacct tggctcaggc gcggcaggcg atggatttca
     3661 tcctgcaaca ccatctgtcc aaatacaacc acgcgcccga actttcagac gatcatcctt
     3721 tacgctcccc atccaagtct gaaaccgtcc tcattatcga ccaaaccttc ggcgatatgg
     3781 ctatccaata tggcggtgca gacgcctcta cgtttgaatt gatgtttcag acggccttaa
     3841 atgaaaaccc gcaagccgat atttgggtca aaacccatcc cgatgttttg tgcggcaaaa
     3901 aacaaggcta tctgacccag cttgtgcagc aacaccgcgt ccatcttctg gcagaagaca
     3961 tcaatccgat ttctttgttg caaaacgtcg ataaagttta ttgcgttacc tcgcaaatgg
     4021 gttttgaggc gcttttgtgc ggcaagccgc tgaccacttt cggcctgccg tggtatgccg
     4081 gatggggcgt gagtgacgac cgccacccaa aaatcggcag ccttattcaa acccaacgcc
     4141 gcgccccccg caacttgctg caactcttcg ccgcagccta tctgcaatac agccgctacc
     4201 tcaatcccaa taccggcgaa gcaggcagcc tctttgatgt catcgactat ctggcgacgg
     4261 tcaaacgtaa aaacgacaaa ttgcgtggcg agttatattg cgtcggtatg tctttgtgga
     4321 aacgcgcggt tgccaaaccg ttctttaacg taccctcttg ccgtctgaaa tttatctctt
     4381 ccacccaaaa actggcaagg gtcaaactgt ccgacgatgc acgcatcctg gcttggggca
     4441 acggcaaaga ggccatcgtc cgctttgccg aacaacacca catccccctg ctgcgcatgg
     4501 aagacggctt tatccgctcg gtcggactcg gctccaactt agtgccgccg ctgtcgctcg
     4561 ttaccgacga tatgagcatt tatttcaatg ccgaaacccc gtcccgtctt gaatacatcc
     4621 tacaaaacca aaacttcgac gatcaagact ttcagacggc cttgaagctg caaaaaatgc
     4681 tgaccgaaaa ccacatcagt aaatacaacg tcggcagctc agacttcacc gccccgtcaa
     4741 ccgacaaaac cgtgatcctc gttcccggcc aggttgaaga tgatgcgtct atccgctacg
     4801 gttcgcccca aatctaccgc aatctggatt tgctccgtac cgtacgcgaa cgaaacccca
     4861 atgcctatat catctacaaa ccgcatcccg atgtagtcag cggtaaccgc atcggccata
     4921 tttcccctga tgatgctgca cgatatgccg accaaaccgc cgaacaagcc gacatcctga
     4981 cctgtctcca atacgcagac gaaatacata ccatgacttc gctgaccggt tttgaagcct
     5041 tgttgcgcgg caaaaaagtc agctgctacg gcctgccttt ctatgcaggt tgggggctta
     5101 cccaagatct gctccctatt ccgcgccgca gccgcaggct tgagctttgg cagctggttg
     5161 ccggcacgct catctactat cccgactaca tccaccccaa aacccatcag gccatcaatg
     5221 cggaaactgc agcccaaatc ctgatacgac aaaaaaatat gcaaaaaaac aacaacggat
     5281 tacatcgcgg gtgctttgca aaaaaattag gtaaaatcaa acaactatat cgatctttca
     5341 aataaatacc atcaaagtta acgatgcgtc ataaacttgc ctctattgcg gcatcattgc
     5401 ctttgcatcg ttaattctct tggcgtatgc ttgaaagttc aacctaaaac tattacataa
     5461 aaaacaaaac cacattgcaa catgaaacag accgtcctca aaaataacct gcaaaacctg
     5521 cttgaaagcg cagaaaatat cctgctgctt caaggccctg tcggcgattt ttttctgcgc
     5581 cttgccgact ggctgactgc aaacggcaaa accgtacata aattcaactt taatgcaggc
     5641 gacgactatt tttatccgcc cactcaagcg cataccgttg tttttaacga caactacgat
     5701 gcctttcctg agtttttgca agaatacatc actcaacatc acatccaggc cgttgtctgc
     5761 tttggcgaca cacgccctta tcacgtcatt gcaaaacgca ttgcaaacga aaaccaagcc
     5821 agtttctggg cgtttgaaga aggctatttc cgcccctact acatcacctt agaaaaagac
     5881 ggcgtcaacg cattttcccc gttgccgcgc cgtgccgact tttttcttga acaattccct
     5941 aagcttgccc agcaagaata taaagcgcca acgccggtac acggcggttt tacgcccatg
     6001 gcaaaaaacg ctatccgtta ctatatcgag ttgttccgca atctacgcaa ataccccgac
     6061 tacatccacc accgcgcacc caatgccggc cattacctca aaccgtggtc gctctccatc
     6121 ctcaagcgtt tgaactacta tattgaagac atccaaatcg ccaaacgtgt ggaagcaggc
     6181 aaatacggca agttttttat tgttcccttg caggtattca acgacagcca agtccgtatc
     6241 cattgcgact ttcccagcgt ccgcagcttc ctgctccatg ttttgagttc atttgccgaa
     6301 cacgcgcctg ccgataccaa catcatcatc aagcatcatc ctatggaccg cggctttatc
     6361 gactactggc gcgacattaa acgctttatc aaagaacacc ccgaactcaa aggccgtgtg
     6421 atttatgtcc atgatgtccc cctgcccgtt ttcctgcgcc acggtctcgg catggttacc
     6481 atcaacagca ccagcggcct gtccggactg attcacaata tgccagttaa ggttctcggc
     6541 cgtgcctatt atgatattcc cggcattact gaccaaaata ccttggcaga attttggagt
     6601 catccgacac cgcctgacaa agagctgttc catgcctacc gaatgtacca cctcaacgtg
     6661 acccaaatta acggcaactt ctacagtcag gtcttttttc ctaacaaaaa cacatccgac
     6721 tcttccacac caacaaccta acttggcgaa agaaaagctc aggccgtctg aaaacatttc
     6781 agacggcctg aaacaatcaa taccttagct actgccatgt aaataaaaca caaaaatctg
     6841 catttatcat taacaataaa ttacaaaaac agtataatga ccgagctgcc atgagcgcat
     6901 accgactcaa cctgagccct ttgtaacaca caaaatatgg atatatccct aggcaaaaca
     6961 atataacaag ccaaacatcc taaagataag ccggcaaggc aatacactct ataaaactgt
     7021 gccgagcaaa atttttacaa agccctcaac cggtatcgcc gcccatatgc cgcagcatcc
     7081 gtcttccact ttatatccgc ccgcaaacca tgaccgccgc tcctgatatc ctctaccggc
     7141 aagccgccgc ccttttggaa caatccaata ccgcccaagc cctgcccctg ttgcaacagg
     7201 cggcagagca aggttatgcg gaagctgctt tcgtattggg caaccatctg ctgcaaaacg
     7261 gccaaccgga gcaggcactt tcatggttgg aagccgccgc ggcccaacgc catcccaaag
     7321 cactcttctc cctgctgcaa caacgcgaac acaacggcac cccgaccgga cagcttctca
     7381 acgactatgc ctggctgggt gagcaggggc actcagaagc ccaattaatc ctcatgcgtt
     7441 accacgcgca acgcaacgat ccacaatcgc tctactgggc ggaacttgct gccgcccgat
     7501 atgccgcacc tgcgtattac catctggcac gccatcatca acgccaaggc gacgttgaaa
     7561 cagccatcga acaatacgaa aaagcggcag cactcggcgt aactgccgcc tgctggcaac
     7621 ttggtcaaat ctacttctac ggtacaggtg tcagccccaa ccacgcacaa gccgaacact
     7681 atctcgaacc agccgcacaa gccggccaca tcgccgcaca aacgctgctg gctgaccttc
     7741 ttgccgccca acgcaaacct gaagccttgg aatggtatcg tcgtgccgcc gataaggaac
     7801 aagcggaagc acagtctaag ctggcccaat acgccctgac cggcgaactt tccgaacgcg
     7861 atccgttcca agcggcacga tatgccaaag ccgctgccga gaaagaccat cctgaagccc
     7921 tgaaaatcat gggcgacctc taccgctacg gcctcggcat caaagctgac aaccatatcg
     7981 cgcacgatta ctaccaccgt gctgccgcgc tgggttctgc cgccgcagca caaaaactca
     8041 tcagcgacgc cgcgctgtac catccgcaac aatacgaaca aatcaaaact gccgccctgc
     8101 aacagcaaca aaccgaaacc atctaccgtt tggcggaagc acaagcctgc gccatcggcc
     8161 gtcccgccga ctacaatgcc gcgcgaaaaa attacatgga agctgccggg ttccaccata
     8221 aaaacgcagc ggcagcctta ggccgcatct accattacgg cctcggtacg gcgcaagatc
     8281 ctcgggcggc tgcacactgg tacgccattg ctgccgaaca aaaccaccct tccgcccaat
     8341 accacctcgc ctgtttttac tatcacgggc aaggtgtcgg ctgtcatgtt ccgaccgcct
     8401 gctactggct gcaggccgcc atcggcaacg gccacacttc ggccgaatca ttaatatccc
     8461 tattagaaca atggcgacgc gaagcacacc atgccatcgg acaaaaggcc gtctgaaaag
     8521 atttacactc gcattttttg acaatcttta actattcccc taatatttgc cagttatttt
     8581 tcacggacac gccattgttt tcatttcttt ctgaaaacac cttgtccgcg catcaatacc
     8641 atgacactcg gcggataacg ccaagcgttg aaacacacta catccggaac aaaaacggat
     8701 gctcggaaaa atatttctag gaggtgaaac aacatggaat gggaattcaa cagttattac
     8761 acactgattg ccgccacgct cgtgttgctg gttggtaaat ttctggttca aaaaatcaaa
     8821 ttcttacgag acttcaatat tcccgagccg gtcgctggcg gtttggttgc cgcgattatc
     8881 ctgtttgcgc tgcacgaggc gtacggcgtg agcttcaaat ttgagaaacc gctgcaaaat
     8941 gcgtttatgc tgattttttt cacgtccatc ggcttgagcg cggatttttc ccgtttgaag
     9001 gcgggcggtt tgccgctggt ggtttttacc gcgattgtgg gcggatttat cttggtgcaa
     9061 aactttgtcg gggtcggact ggctacggct ttgggtttgg acccgctcat cggtctgatt
     9121 accggttcgg tgtcgctgac gggcggacac ggtacgtcag gtgcgtgggg acctaatttt
     9181 gaaacgcaat acggcttggt cggcgcaacc ggtttgggta ttgcatcggc tactttcggg
     9241 ctggtgttcg gcggcctgat cggcgggccg gttgcgcgcc gcctgatcaa caaaatgggc
     9301 cgcaaaccgg ttgaaaacaa aaaacaggat caggacgaca acgcggacga cgtgttcgag
     9361 caggcaaaac gcacccgcct gattacggcg gaatctgccg ttgaaacgct tgccatgttt
     9421 gccgcgtgtt tggcgtttgc cgagattatg gacggcttcg acaaagaata cctgttcgac
     9481 ctgcccaaat tcgtgtggtg tctgtttggc ggcgtggtca tccgcaacat cctcactgcc
     9541 gcattcaagg tcaatatgtt cgaccgcgcc atcgatgtgt tcggcaatgc ttcgctttcg
     9601 cttttcttgg caatggcgtt gctgaatttg aaactgtggg agctgaccgg tttggcgggg
     9661 cctgtaaccg tgattcttgc cgtacaaacc gtggtgatgg ttttgtacgc gacttttgtt
     9721 acctatgtct ttatggggcg cgattatgat gcggcagtat tggctgccgg ccattgcggt
     9781 ttcggcttgg gtgcaacgcc gacggcggtg gcaaatatgc agtccgtcac gcatactttc
     9841 ggcgcgtcgc ataaggcgtt tttgattgtg cctatggtcg gcgcgttctt tgtcgatttg
     9901 attaatgccg cgattctcac cggttttgtg aatttcttta aaggctgatt ttccgccttt
     9961 ccgacaaagc acctgcaagg tttaccgcct gcaggtgctt ttgctatgat agccgctatc
    10021 ggtctgcacc gtttggaagg aacatcatgt atcggaaact cattgcgctg ccgtttgccc
    10081 tgctgcttgc cgcttgcggc agggaagaac cgcccaaggc attggaatgc gccaaccccg
    10141 ccgtgttgca aggcatacgc ggcaatattc aggaaacgct cacgcaggaa gcgcgttctt
    10201 tcgcgcgcga agacggcagg cagtttgtcg atgccgacaa aattatcgcc gccgcctacg
    10261 gtttggcgtt ttctttggaa cacgcttcgg aaacgcagga aggcgggcgc acgttctgta
    10321 tcgccgattt gaacattacc gtgccgtctg aaacgcttgc cgatgccaag gcaaacagcc
    10381 ccctgctgta cggggaaacc gctttgtcgg atattgtgcg gcagaagacg ggcggcaatg
    10441 tcgagtttaa agacggcgta ttgacggcag ccgtccgctt cctacccgtc aaagacggtc
    10501 agacggcatt tgtcgacaac acggtcggta tggcggcgca aacgctgtct gccgcgttgc
    10561 tgccttacgg cgtgaagagc atcgtgatga tagacggcaa ggcggtaaaa aaagaagacg
    10621 cggtcaggat tttgagcgga aaagcccgtg aagaagaacc gtccaaaccc acgcccgaag
    10681 acattttgga acataatgcc gccggagggg atgcagacgt accccaagcc ggagaagacg
    10741 cgcccgaacc ggaaatcctg catcctgacg acggcgagcg tgccgatacc gttaccgtat
    10801 cacggggcga agtggaagag gcgcgcgtac aaaaccagcg tgcggaatcc gaaattacca
    10861 aactttgggg aggactcgat accgacgtgc aaaaagagtt ggtcggcgaa caacgcaagt
    10921 gggcgcagga aaaaatcagc aactgccgac aagccgccgc gcaggcagac cggcaggaat
    10981 acgccgaata cctcaagctg caatgcgaca cgcggatgac gcgcgaacgg atacagtatc
    11041 ttcgcggcta ttccatcgat taggggcaaa ccgatgaata ccgtcccaaa aagcaggatt
    11101 cccgtcaaac cgctgcccga aaaaaccaca gacgaagcca aagtcgaaaa atggcggcag
    11161 ctcggtgcgg aacacggttt gtcgggcgaa tgggcagttg ccgtcagatt gggcgaaaac
    11221 ggttttaccg aagaacagat ggaaaatatc gccaacctgt tcggcagata aagagaaaat
    11281 tgacggaaat gccgtctgaa accctgttat cggtttcaga cggcattttg accaatacgg
    11341 tacgcaggcg caaaacagcc ggcttttcct gtgttgccta tgctgatgtt tcaacacaca
    11401 ggacgataca aaaaacgtcg ccctatgtgc cgtcctgatt cggaagggtt acgctccttc
    11461 caaatatagt ggat
//
LOCUS       DAWKQB010000055        11406 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.055, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000055 DAWKQB010000000
VERSION     DAWKQB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.055"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(61..1206)
                     /gene="dapE"
                     /locus_tag="WJL14_001100"
     CDS             complement(61..1206)
                     /gene="dapE"
                     /locus_tag="WJL14_001100"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ7656178.1"
                     /translation="MTETRSLELAKALISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELSVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQSFLTHAGKLTDVARAAIAEICGVETELSTTGGTSDGRFIKA
                     ITKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(1306..1926)
                     /locus_tag="WJL14_001101"
     CDS             complement(1306..1926)
                     /locus_tag="WJL14_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ7656179.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGA"
     gene            complement(1960..2469)
                     /locus_tag="WJL14_001102"
     CDS             complement(1960..2469)
                     /locus_tag="WJL14_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7656180.1"
                     /translation="MNPFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIARYFNVAAPLHHEDEEEDFFPLLLQYAPQAREGVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYIPDAEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(2564..3115)
                     /gene="azu"
                     /locus_tag="WJL14_001103"
     CDS             complement(2564..3115)
                     /gene="azu"
                     /locus_tag="WJL14_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ7656181.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAGEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(3396..3818)
                     /locus_tag="WJL14_001104"
     CDS             complement(3396..3818)
                     /locus_tag="WJL14_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ7656182.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     VCIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            3949..6699
                     /gene="secA"
                     /locus_tag="WJL14_001105"
     CDS             3949..6699
                     /gene="secA"
                     /locus_tag="WJL14_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ7656183.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDMQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSKLLTQAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMKEIR
                     FDVIGGLVDIYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNTIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            6843..8615
                     /locus_tag="WJL14_001106"
     CDS             6843..8615
                     /locus_tag="WJL14_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ7656184.1"
                     /translation="MIPSDFIDELLAKVDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQIIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLTDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            8802..10730
                     /gene="rpoD"
                     /locus_tag="WJL14_001107"
     CDS             8802..10730
                     /gene="rpoD"
                     /locus_tag="WJL14_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ7656185.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            10877..>11406
                     /locus_tag="WJL14_001108"
     CDS             10877..>11406
                     /locus_tag="WJL14_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7656186.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDRVIDWQLIEQYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPKLEHSLITRIDFNLFCLSIPDYSTLCR
                     YRNWLAQDDTLSELLELINRQLTEKGLKIEKASAVVDATIIQTAGSKQRQAIEVDEEG
                     QVSGQTTPSKDKDARW"
BASE COUNT         3002 a         3206 c         2805 g         2393 t
ORIGIN      
        1 caaatccacg ttaggtctaa tcaaatggtc ggacatccgt atccggcaag caagctgctt
       61 tcagacggca tttccagcca acaaacgcgc caatattccc tcatacaccg ccgacagctt
      121 cggaatgtcg tttagccgca cgttttcgtt gatttggtgg atggtggcgt tggacggacc
      181 taattcgata agctctttcg taatggcttt gatgaagcgt ccgtccgaag tgccgccggt
      241 ggtggacaat tcggtctcta cgccgcagat ttcggcaatg gcggcgcgtg ccacgtcggt
      301 cagtttgccc gcgtgggtca ggaaggactg ccccgaacac gaccactgca aatcgtattg
      361 cacaccgtgt ttgtccaaaa tggcgtggac gcgttgtttc agccctgctt cggtggactc
      421 ggtggagaag cggaaattga atttgacgct cagctcgccc ggaatgacgt tggtcgcgcc
      481 tgtgccgccg ttgatattgg aaatttgaaa gctggttggc gggaaatatt cgttgccttc
      541 gtcccagact tcctgcgtca gctctaacaa ggccggggca aaagtatgca cgggattgat
      601 tgccaaatgc ggataggcaa tatggccttg cttgcctttg acggtcaggt tgcccgacag
      661 cgagccgcgc cgaccgtttt taatcatatc gcccaatttg tccacggcgg tcggttcgcc
      721 gacgatgcag tagtcaatca actcgccgcg cgctttcaat acatcgacga ctttggtcgt
      781 gccgtccagc gcgtcgcctt cttcgtcgga agtaatcagg agcgcgatgc tgccttgatg
      841 gtcggggtgt tcggcaacaa agcgttcgca ggcggtaacg aaacaggcga tgctggtttt
      901 catatccgcc gcaccgcgcc cgtataatct tccgtcgcgc tcggtcggct caaacggggg
      961 cgaatcccat ttttcaacag ggcctgtcgg cacaacgtcg gtatgccctg caaaacagac
     1021 gacgggaact ttcgtgccgc gtcgcaacca gatgtttttg gtgtcgccga aatggagttc
     1081 ttcggccgca aaaccgattt tgtgcaggcg ttcggcaagc agtttttggc aattttggtc
     1141 atccggcgta acagacggac gggaaatcaa cgctttggca agctctaggg atcgggtttc
     1201 ggtcattttt cggcttttct gtttgagatg ccgtctgaac ggcgtttcag acggcatttg
     1261 atgttgaatg gaacgggcta acgatagcga agcgggttct tcaggtcaag caccatgaca
     1321 cgcttcgtaa agcggcagca aatcttccac cgtttccgct atccatttcg cgacatcctg
     1381 cctgcccaaa tcgtcgcgtt cgatgtgttt gccgatgcag aaaaagtctt cgtcgttttg
     1441 caactttcgg tcggactcgt tttgttgcgc gacggtgcgg tagtcgtcgt attcgctttc
     1501 cgcaccgtgc cacatatcga aagaagcgta tttttcggta tcaaaattat ccaaccagcg
     1561 gttgtaatca ggcagcgcga tgggggaaac atcggcttta tagcagtgcc aatccaagct
     1621 gacgctcaga cggcggcggt tgagcagtat cgacaaaatc gctgcggaat ttttatattg
     1681 ttcgtatttg aagtaggcaa agaaatgggc gcgaacctgc cagccgttac accagcgttc
     1741 gatgtgcggc ggcgcaaacg gcgcacccaa ttcggcggca acctgctgaa tcagctgctg
     1801 ccatatctgc cagttttctt tatagtcagc cttgatttgc ggaatgcttt caggccggta
     1861 ttttttaagc tgggcaaact ggaaaaacgg gatattgaac aaatcgcaac ttttcggggt
     1921 cagcataata tatccttgag acgattgttt cagacggcat tatttgcgcc gacgcgccgc
     1981 catgatttca ccgatttcgg tcagtttttc ttttgggata aaggtgttgc ccatatcaaa
     2041 cagcggctct tcaatcgcca gatgcacatc gtaacctgcc acaaagcgtt tgaatgcttc
     2101 ggcatcgggg atataggcat tgtctgcttc gagcttggca aattcggcgg aaacagccgc
     2161 ccagttgtcg tgcagcccga tatgttggcg taaaagctcg tccacacctt cacgtgcttg
     2221 cggcgcgtat tgcagcagca gcgggaaaaa gtcttcttct tcgtcctcat ggtgcagcgg
     2281 cgcggcaacg ttgaaatacc gggcgatttg gcggatggtt tgcaaaacaa tctgattgca
     2341 gccgttttcg gcgatgtagt ccgacagcat cgcgacttgg ccgcaaaaac ggcgtacttt
     2401 gccgtggcag gcatacagca tttcaatcgg ttcggcaaag gtaacgcttt gggtttcaaa
     2461 cggattcatg ttttcgttct caacgggcac ttttcaagca gtcattttat aataaaacag
     2521 cctgcacaaa gcaggctgtc cgtcttttga gactttaagc ggattaatcg accaaagtca
     2581 ctttgccgtt catcaaagca ccgtgacccg ggaaggtgca ggcaaatttg tagtcgccgt
     2641 cggccaattt agcaggatcc agagtcaggg aagcttcttc gccgccgccg atcagtttgg
     2701 tatgggcaac aacgcgtgca tcatcaggtt tgacatagtc ggtatcggca gcacctacgc
     2761 cgtctttaaa tacgccgtcc atgtcttcag ctttggcaat cacgagattg tgacccatgc
     2821 tggctttggg ttgcgtaccg gtatgtttca gagtgatggt gaactcttta catgctttgc
     2881 tgacttggat gtctttggtg ttgaactgca tattgtcgtt ggattcgaca gttgccgcac
     2941 agttgccggc agcaggggct tcggcagcat ctgcaggagc agcttcggcg gcaggcgctt
     3001 cggaagcggg tgcttcacca gcaggagttg cctcggcagc aggcgcggca ggttcttgag
     3061 agcaggcagc caaaccgata acggcggcag aaatcagagc cagatacgct ttcataacaa
     3121 atctccaatc gataaaataa tattcggttt tacagaaatc aaagtgcaac cgccattaac
     3181 aaaaccttga aaaagattcc gccgcgttgc acaaacagat gtttcggagc ggcattttgc
     3241 tacaaatttc atttgaaatc aaagcctgtc tgcaagttta caatcgttta ctttaaaagg
     3301 ggcaatttta ccccgaacct atttctttag tattagaccc attatccttt acttcttaat
     3361 attaacggat gtttacacaa attcccgtat acattttatg cgccatgcct tctaaccaag
     3421 tttgccaatg cctccgccaa ttcgggatgc cgtttttcca actttaccgc cgccgaaccg
     3481 aaactctcca gcgcagcctt actcaaatgc agggtattgg ttttcggcgg tttttccggt
     3541 ttcgggacca gcctgaccga aacagagcgt atcgaagcgt caagccctgc caactgcggc
     3601 aataccgacg gtgcaatcat tttcaagcgc gatgccgcca tattgtttgc cgccaaaagg
     3661 acaagcctgc cgtcttcgat acataccgtc tgaaaatgcg ggtgcaggtt ggcaggcagc
     3721 agttttttca cggcggcatc caaccgccgc cactgtcccg cctgtttcaa aagtccggaa
     3781 agcagcgcgt cccgcctgcc caactgttcc aaattcataa aacatacacc caaaaagatt
     3841 gaaataccgc aaacgcgcct ttatttcaga cggcattagc actttgcaca aacgcttgtg
     3901 ttaaaatcgc gttttcgccc actattatat caggcgcagg aattattcat gctgacaaac
     3961 attgccaaga aaatcttcgg cagccgcaac gaccgcttgc tgaaacaata ccgtaaatcc
     4021 gttgccagaa tcaacgcgct cgaagaacag atgcaagccc taagcgatgc tgatctgcaa
     4081 gccaaaactg ccgaattcaa acaacgcctc gccgacggtc agactttgga cggcattttg
     4141 cccgaagcct tcgccgtctg ccgcgaagcg tcccgccgca ccctcggtat gcgccacttc
     4201 gacatgcagc ttatcggcgg tatggtgctg cacgacggca aaatcgccga aatgcgtacc
     4261 ggcgaaggca aaaccctggt cgccaccctc gccgtctatc tcaacgcgct ggccggcaaa
     4321 ggcgtacacg tcgttaccgt caacgactac ctcgcctcac gcgatgcggg cattatggag
     4381 ccgctctata atttcctcgg ccttaccgtg ggcgtgatta tttcagatat gcagccgttc
     4441 gaccgtcaaa acgcctatgc cgccgatatc acctacggca ccaataatga attcggcttc
     4501 gactacctgc gcgacaatat ggttaccgac caatacgaca aagtgcagcg cgaattgaat
     4561 tttgccgttg tcgatgaagt ggattccatc ttgattgacg aagcgcgcac tccgctgatt
     4621 atctccggcc aggcagacga caacatccag ttgtaccaaa tcatgaacac cgtcccgccc
     4681 cacctcgtcc gccaagaaac cgaagaaggc gaaggcgact actgggtaga tgaaaaggca
     4741 catcaggtca tcctgagcga agcaggtcac gaacacgccg agcaaatcct gacccaaatg
     4801 ggattgctgg cagaaaacga ctcgctctat tccgccgcca atatcgccct gatgcaccac
     4861 cttatggcgg cattgcgcgc gcattccctc ttccacaaag accaacatta cgtcatccaa
     4921 gacggcgaaa tcgtcatcgt ggacgaattc accggccggc tgatgtccgg ccgccgctgg
     4981 tcggagggtc tgcatcaagc cgtcgaagcc aaagaaggcg tggaaatcaa acgcgaaaac
     5041 caaaccctcg cctccatcac cttccaaaac tatttccgcc tgtacaccaa gctctccggt
     5101 atgaccggca cagccgatac cgaggctttc gagttccaaa gcatctacaa cctcgaaacc
     5161 gtcatcattc cgaccaaccg ccccgtacag cgcaaagact tcaacgacca gattttccgt
     5221 tccgccgaag aaaaattcga agccgtcgtt aaagacattg aggaatgcca caaacgcggg
     5281 cagcccgtcc tcgtcggcac caccagcatt gaaaactccg aactggtatc caagctgctg
     5341 acccaagccg gactgccgca caacgtcctc aacgccaaag aacacgaacg cgaagccctg
     5401 attgtcgccc aagccggcaa agtcggcgcg attaccgttg ccaccaatat ggcgggacgc
     5461 ggtacggaca tcgttttagg cggcaacctg aagcaccaaa ccgatgccat ccgcgccgac
     5521 gaaaccttga gcgacgaaga gaaacaggca caaatcgccg cactcgaaga cggctggcag
     5581 gcggaacacg acaaagtgat ggaagcaggc ggtttgcaca tcatcggtac ggaacgccac
     5641 gaaagccgcc gtatcgacaa ccaattgcgc ggacgttccg gccgtcaggg cgaccccgga
     5701 tccagccgtt tctacctctc gtttgaagac ccgctgctgc gcctattcgc actcgaccgc
     5761 gccgccgcca tcctcaaccg tctcgccccc gaacgcggcg tcgccatcga acacaacctg
     5821 ctgacacgcc aaatcgaagg ggcgcaacgc aaagtcgaag gcagaaactt cgatatgcgc
     5881 aaacaggttt tggaatacga cgacgttgcc aacgagcaac gtaaagtcat ttacagccaa
     5941 cgcaacgaaa ttttaaccag caaagacatc agcgacctga tgaaggaaat ccgttttgat
     6001 gtcatcggcg gacttgtaga catatatatg ccgccggaca gcatggaaga acaatgggac
     6061 attccgactc tggaaaaccg tctggctgcc gaattcagac tgcacgaaga cattcaatcc
     6121 tggctgaaag cagacaatac aattgacggt caagacatca aagagcgcct gatcgaacgc
     6181 atcgaaaacg aatatgccgc caaaaccgaa ctggtcggta agcaggcaat ggccgatttc
     6241 gagcgcaacg tgatgttgca ggtcatcgac aaccaatggc gcgaacacct cgccgctatg
     6301 gactacctgc gacaaggcat acacctgcgc agctatgccc aaaaaaatcc gaagcaggaa
     6361 tataaacgtg aagcctttac catgttccaa gacctgtgga acggcatcaa attccatatt
     6421 gcctccctgc ttacctcggt tcaaatcgaa caaaaccctg tcgcggtggt tgaagagcaa
     6481 cccatcggca acatccagtc catccattcc gaatcgcccg atatggaaga acttttgggt
     6541 cagtcgcaaa ccgatctggt taccgaagcc tttaatcccg atggaacgga tttcagcccc
     6601 gaagccttgg aagcgcgggg acaaatcgtc caccgcaacg acccctgccc ctgcggcagc
     6661 ggtttgaaat acaaacaatg ccacggcaaa ctggcttgag cgtttgaacg caaatgccgt
     6721 ctgaacatcc ggtttccggc ttcagacggc attttgcctg aaccgccaca tccgactgcc
     6781 attccgaaaa atcccgattt cgtaccgtcc aaactaaaaa caggcatccg ccccgccgaa
     6841 ctatgattcc atccgacttc attgacgaac ttctagccaa agtcgatatt gtcgatatta
     6901 tcgacgagca ggttccgctg aaaaaaggcg gggcgaacta tatggcgtgt tgcccgttcc
     6961 acaaggaaaa aacaccgtcg ttttcggtca gtccgaccaa gcagttttac cactgtttca
     7021 gttgtggggc gcacggttcg gcgattggtt ttgtgatgga acatcagggg ctgtcgtttc
     7081 cggaggcggt gcagttcctt gccgaccgcg tgggtatgat tgtgcctaaa gtgcgcgggc
     7141 aaaacgataa tcccgaagtc cgtgccgagc gcaagaaaaa acagcagacg ctggaggaaa
     7201 cgacggctgc ggcagctgat ttttatgcgc aacagttgaa attcaatccg acggcgaagg
     7261 cttatttgga caagcgcggc ttgagcgcgg aagtcatcgc gcattatggt ttgggctacg
     7321 cgcccgacgg ctggcagcct ttggcgcaag tattccaacc gtacccgaat accgcgttgg
     7381 tggatacggg gatggtgatt gacaatgagg ggcggtatta cgaccgcttc cgccatcgga
     7441 ttatgttccc tatccgcaat ccgcgcgggc agatcatcgg tttcggcggc agggtgctgg
     7501 acgactccaa acccaaatat ctaaactctc ccgatacacc tttgtttgac aaagggaaaa
     7561 acctttacgg actgtatgaa gggcgtgccg ctgtcaagga agcggggcgg attttggtgg
     7621 tcgaaggcta tatggacgtg gtcgcgctgg cacagttcgg cgtgggctac ggtgtggcgg
     7681 ccttgggcac ggcaacaaca gcggaacacg tcaaaatcct aatgcgtcag gcagacagta
     7741 tttatttctg tttcgacggc gacagcgcgg ggcgaaaagc ggcttggcgc gcgctggaaa
     7801 acgcgctgcc gcagttgaag gacgacaaat cgctgcattt tttgttcctg ccggaagaac
     7861 acgaccccga cagctacatc cgcgcctacg gcaaagcgca atttgaagat gcgctgttga
     7921 atcaaagcaa gcccttgtcg gaatattttt gggaacatct tacagacggc attcatctca
     7981 atacgcagga aggcaaggcg gaattggtaa aaaccagttc gccgcttttg gcgcagatta
     8041 ccgcgccggc attggcttat ttgttaaaac aacggcttag cgagctggtc ggcatcgacc
     8101 ccgacaacct cgcgcaactg ctcggacagg aagcgccaaa gcggcacgtc aaacaaaaaa
     8161 actacaaact gcccccgatt tccgtcaaac agcccgttat gccgacattg gtgcaacggc
     8221 aaatccgcag cctcttgata aatccggatt gggctgcata tatagacctg cccgattatt
     8281 tggcgttgga cggcgatttc gcctgccttg ccaacctcgc cgaaaccatt aaaaaccatc
     8341 cttccgtgcc ggcaaccgca caggttttgg aacatatgcg cggttcgccc tacgaagaaa
     8401 caatcaaccg catcttccgg tcggcccttc aatcggaaga aatggaaggc ggcggcgaag
     8461 aagattgcga aaacttccaa atcggcatca aaaaactgct caatgagtta aaatacagcc
     8521 aaatcgaaac attaaaacaa aaaagcctgc aatccggctt aaatgaaagc gagaaaaaac
     8581 ttttgctgtc gctgctgacc gcaaaacaaa attgaccggc ggattccgcc atccgtaaac
     8641 cgttatgccg tctgaaaagc attcaccccg gctgcaacaa cgacacctgc agaacaccca
     8701 tccccaaaag ccttcagacg gcatcagagt accctactct gccacgcctt caggtgcgtc
     8761 caaacgcaaa ccgtcggcat cttgccaaca gaaagcagac aatgtccaaa aaccaaaatc
     8821 acgaagaata ccaagacgat gcccgccccc tgacgattga ggaacaacgc gcgcgcctgc
     8881 gccaactcat cattatgggc aaagaacgcg gctacatcac ctactccgaa atcaatgacg
     8941 cactgcccga cgatatgtcc gatgccgatc aaatcgacaa catcgtcagt atgatttccg
     9001 gtttgggtat ccaagttacc gaacacgccc ccgatgcgga agacatattg ttaagcgaca
     9061 atgccgccgt taccgacgat gatgccgtcg aagaagccga ggccgccctt tccagcgccg
     9121 actccgagtt cggcaggacg accgaccccg tccgtatgta tatgcgcgaa atgggacagg
     9181 tcgacctgct gacccgcgaa gacgaaatca tcatcgcaaa aaaaatcgaa aacgccctga
     9241 aaaatatggt tcaggccatc tccgcctgcc cgggatccat tgcggaaatc ttagaactca
     9301 tcgaaaaaat ccgcaaagac gaaatccgcg tcgacgaagt cgtagaagcc attatcgacc
     9361 cgaatgaagt attgctcaac gaattgggct tggggcactt ggaaaccaca gcgcccgaga
     9421 aaccttccaa cgacaattcg gacgaaaacg aagacgacga agaatcggaa gaagatgcgg
     9481 atgaaatctc ggcagccaat ctcgccgaat tgaaacaaaa agtcatcggc cactttgccc
     9541 aaatcgaaaa agactacaaa aaaatgatcg gtcgtttgga aaaacaccac agccggcaca
     9601 aagactatct cgcctaccgc gacgcgattg ccaacaaact gctggaagtc cgtttcgcca
     9661 cccggcaaat cgacagcctc agcagcagcc tgcgcggaaa agtagaaaac atccgcaaac
     9721 tcgaacgcga aatccgcgac atctgcctcg accgcgtcca tatggagcgc gactacttca
     9781 tccaaaactt cctgcccgaa atcaccaatc tggaatggat tgaagaagaa atcgccaaag
     9841 gcagggtttg gagcgacgcg ctcgaccgct tccgccacgc catcctcgaa aaacaaaccg
     9901 agttggcaga tatggaaaaa gaaacccgca tttccatcga agagttgaaa gaaatcaaca
     9961 aaaatatggt gtcgagcgaa aaagaaaccg cagccgccaa acaggaaatg attcaggcaa
    10021 acttgcgcct cgtcatttcc attgccaaaa aatacaccaa ccgtggcttg cagttccttg
    10081 atttgattca ggaaggtaac atcggcctga tgaaagcggt cgacaagttc gaataccgcc
    10141 ggggctacaa attctccacc tacgcaacct ggtggatccg ccaggcaatc acccgttcga
    10201 ttgccgatca ggcgcgtacc atccgcattc cggtacatat gattgaaacc atcaacaaga
    10261 tgaaccgcat ctcgcgccaa caccttcaag aaaccggcga ggaacccgat tccgccaaac
    10321 ttgccgaact gatgcagatg cccgaagaca aaatccgcaa aatcatgaaa atcgccaaag
    10381 agccgatttc gatggaaacc cccatcggcg acgacgacga ttcgcacttg ggcgacttca
    10441 tcgaagatgc caacaatgtt gcgccggccg atgcggcaat gtacaccagc ctgcacgaag
    10501 taaccaaaga aatactcgaa agcctgaccc cgcgtgaggc aaaagtcctg cgtatgcgtt
    10561 tcggcatcga tatgaacacc gaccacacgc tggaagaagt cggcagacag tttgacgtaa
    10621 cgcgcgaacg catccgacaa atcgaggcaa aagcactccg caagctgcgg catccgacaa
    10681 gaagcgaccg tttgagaagt ttcttggaca gcgaagacag caagctgtaa accaaaaaac
    10741 cgcaggtttc aaatacctgc ggttttttct tacacaataa acaacgcttc cacatatccc
    10801 acactcctat cccgagacct ttgcaaaatt ccccaaaatc ccctaaattc ccaccaagac
    10861 atttagggga tttctcatga gcaccttctt ccggcaaacc gcccaagcca tgattgccaa
    10921 acacatcgac cgcttcccgc tattgaagtt ggaccgggtg attgattggc agctgatcga
    10981 acaatacctg aaccgtcaaa aaacccgtta ccttagagac caccgcggtc gtcccgccta
    11041 tcccctgttg tccatgttca aagccgtcct gctcggacaa tggcacagcc tctccgatcc
    11101 caaactcgaa cacagcctca tcacccgcat cgatttcaac ctgttttgcc taagcatccc
    11161 cgattacagc accttatgcc gttaccgcaa ctggctggcg caagacgaca ccctgtccga
    11221 attgctggaa ctgattaacc gccaactgac cgaaaaaggc ctaaaaatag agaaagcatc
    11281 cgccgtcgtt gacgccacca ttattcaaac cgccggcagc aaacagcgcc aggccataga
    11341 agtcgatgaa gaaggacaag tcagcggcca aaccacaccg agtaaggaca aagatgcccg
    11401 ttggat
//
LOCUS       DAWKQB010000056        11302 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.056, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000056 DAWKQB010000000
VERSION     DAWKQB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.056"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            56..337
                     /locus_tag="WJL14_001109"
     CDS             56..337
                     /locus_tag="WJL14_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7656187.1"
                     /translation="MIHEEICAMPMGYETLIGDMGSALSGGQKQRIVLARALYCEPKI
                     LFLDEATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVESADRKMSLG"
     gene            complement(358..1584)
                     /locus_tag="WJL14_001110"
     CDS             complement(358..1584)
                     /locus_tag="WJL14_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ7656188.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRKGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            1749..1943
                     /locus_tag="WJL14_001111"
                     /pseudo
     CDS             1749..1943
                     /locus_tag="WJL14_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2128..2787
                     /gene="queC"
                     /locus_tag="WJL14_001112"
     CDS             2128..2787
                     /gene="queC"
                     /locus_tag="WJL14_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ7656189.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2815..3333
                     /locus_tag="WJL14_001113"
     CDS             2815..3333
                     /locus_tag="WJL14_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ7656190.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSENAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3340..3762
                     /gene="queD"
                     /locus_tag="WJL14_001114"
     CDS             3340..3762
                     /gene="queD"
                     /locus_tag="WJL14_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ7656191.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            4031..4402
                     /locus_tag="WJL14_001115"
     CDS             4031..4402
                     /locus_tag="WJL14_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ7656192.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATTLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAMLAAAAVLAV"
     gene            4399..5037
                     /locus_tag="WJL14_001116"
     CDS             4399..5037
                     /locus_tag="WJL14_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ7656193.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTFKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5372..5448)
                     /locus_tag="WJL14_001117"
     tRNA            complement(5372..5448)
                     /locus_tag="WJL14_001117"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5412..5414),aa:Pro,seq:cgg)
     gene            complement(5673..6758)
                     /gene="nagZ"
                     /locus_tag="WJL14_001118"
     CDS             complement(5673..6758)
                     /gene="nagZ"
                     /locus_tag="WJL14_001118"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ7656194.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAIGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMNVLGQIWDKDGASA
                     AETAAGQVGWVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVIQTAD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            complement(6815..8335)
                     /locus_tag="WJL14_001119"
     CDS             complement(6815..8335)
                     /locus_tag="WJL14_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ7656195.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8535..10034)
                     /locus_tag="WJL14_001120"
     CDS             complement(8535..10034)
                     /locus_tag="WJL14_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ7656196.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10172..10801)
                     /gene="nth"
                     /locus_tag="WJL14_001121"
     CDS             complement(10172..10801)
                     /gene="nth"
                     /locus_tag="WJL14_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ7656197.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYKG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(10847..11260)
                     /locus_tag="WJL14_001122"
     CDS             complement(10847..11260)
                     /locus_tag="WJL14_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7656198.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2657 a         3017 c         3146 g         2482 t
ORIGIN      
        1 ttttttcgat acggaaccgg atagaggaaa aatcgaacat tgcgcctgcc ttgctatgat
       61 tcacgaagaa atctgcgcca tgcctatggg ctatgaaacc ttgatcggcg atatgggcag
      121 cgcactgtca ggcggacaaa aacaacgcat cgtattggct cgtgccttat attgcgaacc
      181 gaaaatccta tttttagatg aagcgaccag ccatttggat attgccaatg aaaaagcagt
      241 caatgcaaac ttgaatggct tgtctatcat aaaaattatg gcggcacaca gaaaggaaac
      301 ggtggaatca gcagatagga aaatgtcttt aggataaaaa tacagtttca aaaatactca
      361 agattgctgc tgttttttcg cctgagcgtc aaactctgcc agcgtcatgt tcaaagtctg
      421 caaacacggc atcattaccg catcgacagc ttggttcaca tgatcccttt ccacaggcaa
      481 cggacggtaa acgaagagct tgaagagttc gttcaactca atcgaatccg cccccgtttt
      541 caacacccaa ccctgtctgc cggaataaat gtagccgtgc cgcgccagct tttccaaaag
      601 ctcgcccaac tcgtcgtagc ccatattgat atgccgtctg aactcctgaa caggcaaggc
      661 tttgccttct ttttgcgccg catccagaag cagcaggatt ttcaacacgt cgtcaaaccg
      721 tccgcgcgag tcgaagccct tgcggaacgc ttctccctgc cagtaggaga gtgaagaagt
      781 cagcaccgcg ccgcccaaga ccagcgtcca caacaggttc agccacaaca gaaaaaacgg
      841 cacggcggca aacgcgccgt aaatcgagcg gtagccgtcg aaattgccca tataccaagt
      901 aaagagggaa cgcgcggttt ccagacagaa cgctgttgcc aaagccccga caaacgcctg
      961 ccgcgcggga acgaagcggt ttggcacgaa gcggtacagc ccccacagca aaagcgtcat
     1021 gaaggtcagc gtcgccgccg ttcgcaacgc gcccgaccac tgcggcgcac ctgaggcaag
     1081 cgcggcatcc tgtaccgagc cgaccataaa ggaaatgccc acgcccaaag acagcggccc
     1141 gaacgtcagt aaagcccaat agacgagaaa ctgcatcatc cacggacgct gggaattgac
     1201 ccgccagatg cggttgaacg tattgtctat cgtccgaatc agcatcagcg aggtaacgac
     1261 cagcatcacg ctgccgattg ccgtcagccg gttcgcctgc tcgcggaacg cattgatata
     1321 gtcgaacacc atgtccgcgc cctgcggcac aatggtttgg ttgacgaagg agacgaacga
     1381 atccgaccag cggtcgaaca cggggaaaat cgaagcgacc gccaccatca cggtcagcac
     1441 ggggacgagt gccagcagtg tcgtaaacgt catgcttgcc gccgcctgcg gtacgcgttc
     1501 ttcatcaaag cggcggacga cgaaccatgc aaacgcacag attttattgt ctgccaaacc
     1561 ttgcaaacgt tgtaaaaagg tcataatttc ttgcccggtc agtaagttgg gcattgatgc
     1621 ccgatgttat agccaatttt gccgtcagga acaaatgcct gaactgcggc tgtttcagac
     1681 ggcatcggaa caactgttat gccgtctgaa gaccgaacca ttttaacgga atccgcccat
     1741 gaacccaaat ccccctcaaa atcctcgtcc tctactattt ccaaaacggc agcacccgca
     1801 atcccgcccg ccgaatcgct cgcggcatcg acagcgttga aggttgcgaa gccgtattgc
     1861 gcaccgtccc caaagtgtcc gccgtctgcg aagccgtcga aaaagatatt cccgacagcg
     1921 gccccgtccc gaccgccgaa gaaaacaata tcgccttcgc acaaagcaaa cgcttggcgg
     1981 aactcgccat caagtcggca taagccgcgt gttcagacgg catggcgttc agatgccgtc
     2041 tgaacgcgtt tgcctgtata atccgcgtct ttactgtcca acttcgcggt tcgaaaacct
     2101 cccgcgtcac caaaactagg attcgatatg tcaaaccaaa aagccttggt catcttttcg
     2161 ggcggtcagg attcgaccac ctgcctgatt caggcaatcc aaacctacgg gcgcgaaaac
     2221 gtccaagcca ttactttcca atacgggcaa cgccatgccg tcgagctgga acgtgcccgc
     2281 tggattgcgc aggatttggg cgtcaaacaa accgtactcg acttgagcct gatgcggcag
     2341 attacgcaca atgccctgat ggacgacacc gccgccatcg aaactgccga aaacggcgtt
     2401 ccgaatacct ttgtagacgg ccgcaacgcg cttttcctgc tctatgccgc gatttacgcc
     2461 aaagggcagg ggatacggca catcatcgcg ggcgtgtgcg aaaccgactt ctccggctat
     2521 cccgactgcc gtgacgtgtt tgtcaaatcc atgaacgtta cccttaatct ggcgatggac
     2581 tatgattttc aaatccacac gccgctgatg tatctgacca aggcgcaaac ttgggcgttg
     2641 gcggacgaaa tgggcgcgct ggactatatc cgcgagcaaa cccacacctg ctataacggc
     2701 atcgtcggcg gctgccgcga atgcccgagc tgtatcttgc gcgaacgcgg gctggcggaa
     2761 tatctggaaa gtaaaaaggc cgtctgaaca cgcgcaaacc ataaggaata cgatatgccc
     2821 aagctccata tgttttacct cggcggcaat gccggcaggt cgaatatcga agtgcacgac
     2881 atccaatttg ccgtgtgcga cgactaccgt gaagccgtcc ccgcactcaa agccgcgtgg
     2941 ttcggcgatg cggacaaaat ccacatcgac ggctggcagg ttgtcgaatg ggcggacggt
     3001 tacgacatcg ccgtatccga aacgcccaaa acgaaaatgc cgtctgaaaa cgccccgcgc
     3061 ctgtattttg ccaatgtcgg cggctatcgc gcagggcagc tcgccgaagc gcacgctttc
     3121 gggttgttcg ccgccgccac gcctgccgaa gccaaacaaa aagccctgca aaccctgttg
     3181 accgacagct atgttcagca gcataaagac aacttaaaag acgtggacaa cctgcttgcg
     3241 ctcgaccgca tcggcaattt ccatatccgc ctgaccccga atccgcacgg caaacccgcc
     3301 gaaatcggct ttcagggcta tttgcccatt tgagaaccca tgaaaatcac caaaatcttc
     3361 accttcgact cctcgcatat gctcgacggg catgacggca aatgccaaaa cctgcacggg
     3421 catacctaca aactcgaaat caccgtttca gacggcatta tcaaaggcgg cgcgaaagac
     3481 ggtatggtga tggactttac cgacttgaaa gccattgtca aacaacacat taccgacccc
     3541 ttcgaccacg ccttcatcta ccacggcggc aacagccgcg aatgccaaat cgccgcgctt
     3601 ttggagggct ggaacatgaa aaccctgcgc ctgccctgcc gcaccactgc cgaaaatatg
     3661 gcggtcgaaa tgtacgaccg tctgaaaaac gcggggctga acgtgtgccg cgtgaaattg
     3721 tgggaaacgc cgacatcgtg tgcggagtat gaaggggagt agggaatatc ttgaacgtat
     3781 cgatatagta aattccaata agacatgccc aaccgcgtca ttcccgcgca ggcgggaatc
     3841 cagaccttga tttatcagga atatttaaaa attgcagcaa ttccaactct ctggattccc
     3901 gcctgcgcgg aaaggacggt ttagagcgtc cttatttgaa tttaccgtaa aacggttttt
     3961 tctcctgtac ggattccccg ttttttcaga cgaccttcca tatcaaatac acccattaaa
     4021 aggaataccc atgaaactcc tcttcatcct cctagtcctc ttcgtcgccg tcgaacattt
     4081 ctacatcgcc tggcttgaaa tgacacagat tcccagcgaa aaagcggcgg aaatattcaa
     4141 gctgccttat gaatttatgg aacaaaagcg cgtgcagacc ctgtttggca accaagggct
     4201 gtataacggc tttctcggca tcgggctggt gtggtcgcgg tttgccgcgc cggacaacgc
     4261 cgtttacggc gcgacgactc tgtttctcgg tttcgtattg attgccgccg cgtggggcgc
     4321 gttttcgtcc ggcaacaaag gcatactcgt caaacaagga ctgcccgcga tgctggcggc
     4381 ggcagcggtg ttggcggtat gaaaaaaatc aatgttgccc ccgaaaatcc gcaataccgt
     4441 atcgtcgaaa ttttcgagag cctgcaaggc gaaggctgga acacgggcat gcccgccgtt
     4501 ttcgtccgct tgggcaaatg caacctcgcc tgcggctggt gtgataccga ttatttgaca
     4561 ttcggcatga tgagcctgtc cgacatctta ggctgtctga aaacctatgc cgcccgcaac
     4621 atcatcatca ccggcggcga accgaccata cagccgcatc tcgatatgct gctggacacg
     4681 ttcaaggcgg aaggctattt cctctgtctc gaaaccaacg gactcaatcc cgcgccgccg
     4741 caaatcgact acgtcgccac cagccccaaa gcctgctacg ccgccaaata tgaaaatagc
     4801 tgtatcgaaa cagccgacga agtgcggatt gttgccgatg gtgatgtcct tgcgttctgc
     4861 gaaaacatgg aacgcaaaat ccgcgcacat cattactacc tttcgccctg tgagcaagac
     4921 ggtgcgatga acatctacga caccatccgc caaatcggta ttttaaacag tcgccccgac
     4981 gcatccgtgc attggcagtt gagcgtgcag acgcacaaat gggcgggaat agagtagttt
     5041 aagcagtgta actcaaaggg acggcgtacg gttttaccga tgtttgacat acggggaaag
     5101 tgtgccgctt ctgcgtggaa atgccggcat ttccaccgcc caatcaggac ggagccttac
     5161 tgaataagat gctgccgttg ggtacaagct cggcttccta aattccgatg gtcttttgaa
     5221 ccttgccgat actctgtgcc agtgcgcgca aatggcaggg ttagggaaaa cgaaatgccg
     5281 tctgaaacag cattctgttt cagacggcat ttttctgttg acgtcaaaag gaaaaactgc
     5341 ctcggcaatg gataccgagg cagtttggat ttggtcggaa tgagaggatt cgaacctccg
     5401 accccttcgt cccgaacgaa gtgcgctacc gggctgcgct acattccgaa ttgattggaa
     5461 cgggattata acgcaaaaag tgcggcgtgc ctataccgtt ttgccttttt gccgcgtgtc
     5521 gggcggattt aaaacgttgt gtttgaatac agtgttgata agcatcctta tctccaagta
     5581 attcaataag ataactttct acctgaccga aaaaatcatt gcctttccct gacaaacggt
     5641 tgaaatcggc agattgttga aacgcagccg atttaaaagg cctctccgac ttttacgccg
     5701 cccgccgtgt cttgcggcga ggcaagaccg gcaacaaagg cttgcgccgc ttggaaatcc
     5761 gccgtctgta taacggcttg cgcggcggca ctgccgagcg tgttggccat atattgccaa
     5821 cgttgcgcca aagcgggatt gtcaggaatg cggaaatctt cgcgcagttc atccacaagg
     5881 tcgggacggt tgcagacgag gacgatgtcg caacctgcct caaaggaaat gcgggcgcgt
     5941 tctttgatgc cgcctgcccc gcacgcgcct tccatagtca aatcgtccga gaaaatcacg
     6001 cctttgaacc cgatgtcgcg gcgcaaaatt tgtttgagcc agatttcgga aaacccggcg
     6061 ggctttgtgt ccacttgtgg ataaacgacg tgggcgggca taaccgccgc cataccttcg
     6121 cggctcataa tgcggaaggg aatgaggtcg gcggcttcga gttcgtcgag gctgcgcccg
     6181 tcttcgggta gcaccaaatg gctgtcccct tcgacaaacc cgtgtccggg aaaatgtttg
     6241 ccgcaggatt tcataccgcc ttttgccaaa cctttttgaa gggcgagggc gaggcgggtt
     6301 acggcttcgg ggttactgtg gaaactgcgg ttgccgatga cggggcagtt tccccagtcc
     6361 aaatctaaga cgggcgtgaa ggacaagtcg atgccgcagg cggaaagctc ggttgccaaa
     6421 acccagccga cttgtccggc ggcggtttcg gcggcggatg cgccgtcttt gtcccaaatt
     6481 tgccctaaaa cgttcattgc gggcaggcgg gtgaagcctt cgatgaaacg ttgcaccctt
     6541 ccgccctcgt gatcgacggc gataatgagt tcgggtgtgc gaagtgcttt gatttcggcg
     6601 gtgagtgttt tgagctgttc gatgttttgg aagttgcggc ggaagaggat gatgccgccg
     6661 attgcggggt cgagcaggcg ttgtttttct tctccggtca ggcggaaggc ggcaatgtct
     6721 gccatgacgg ggccgcgcgg aatatggggg acggtcattg cggtttgctc caaaaagctt
     6781 cagacggcat atgccgtctg aacagggaaa ggggtcaggc gttggcgcgt tttttatctt
     6841 tcaacagaaa aatcagcacc gccaatacaa tgcctgtcgt gccaaagccc aacagcgcgg
     6901 attttgtcag acccaatgcg aggtagcccg atgcggcggc ggcggcaacg gttaaggcgt
     6961 aaggcagttg cgaggtaacg tggtcgatgt ggttgcagcg cgctccggtg gacgacagga
     7021 tggtcgtgtc ggaaatgggc gagcagtggt cgccgcatac cgcccccgcc attactgcgg
     7081 acatacacgg gataatcagc gcgggttcga ctttgaccgc catggcggcg gcaatcggca
     7141 gcataatgcc gaacgtcccc cagcttgtgc ctgtggcaaa cgccatcacg ctggcgagca
     7201 ggaagaggat gacgggcagg aagccgggat ggatgttgcc cgcaaccagt gtggagaggt
     7261 aatcgccggt gtgcatttcg ccgacaaccg tactgatgag ccaagcgagg attaaaatgg
     7321 cgattgcgcc gaacatagat ttcgcaccct gccaaacggc tttgggatag tcggcggttt
     7381 taatcgtgcc gagcgtgcag agaacgacgg caaggacgcc gcaagtgccg ccgaatacca
     7441 gcgaagtgtt tacgtccgtg ttttcaaatg cccccaaaat gctgaaggtt tcgcttgcct
     7501 gcgcgccggt gtagatcatg gcggaaaccg ttgaggcgat taaggccaaa acgggaataa
     7561 tcagtgcgta aacacgacct ttggtagcgt ctgaaacggc agtttcatcg tgggcttcgt
     7621 tcaacgcggc ttgttcgaaa cgtgccatcg agccgatgtc gaaggaaaac catgcgacga
     7681 cgaacaccat aatcagggca aacagtgcgt aatagttcat caggctcatg gcgacaaacg
     7741 tccccatcgg cgtgtattcg gtgattttgt aggtaacgag cagtccggca agcgtggcga
     7801 taatcgacgc gccccagctt gaaacgggca tcagcacgca cataggcgcg gcagtggagt
     7861 cgaggatgta ggcgagtttg gcgcgggaaa ctttaaactt gtcggtaacg gggcgggcaa
     7921 tcgcaccgac ggcgagactg tggaaatagt cgtcgataaa ggttacgaac acgaggcagg
     7981 cggtcagcat tttcgcgccg cgccggtttt taatgtgccg ttttgcccag tcggcaaacg
     8041 cctgattgct gccggagtag gtcagcaggg aagtaaaaat acccaaaagt atcaggaaaa
     8101 ccaagatttt tggtttgccc agcgaccaat cgccgtctga ccaagccaag ccgacgacca
     8161 tgtctttcag gtgtgtcaga ccgtcgacgg ggttgccgcc gaccaaaaag gcaacgccga
     8221 ccagaatacc gatgcctaaa gacagcagta cgcggcgggt aatgatggca agtgccagtg
     8281 ccaaaaaggg tggcacaacc gagaaaaatg aatgtgaata gtcgatcagc tgcatggtta
     8341 tgggggtgtt aagcgtccgg atggggcggt ttctgtccgc aacggttcgg attttgttgg
     8401 caaaatgggc gaaagtattt tctgttccgg aaaatactta tttgaaatca attaactgat
     8461 tttttaaaat gcccgttaat cggtattgac gggcatttta tcatttaaaa aatattttgg
     8521 ttaaattatg tgggttattg caggtttaat gcgataaaca gcgtgttgcc acggcgcatg
     8581 accagcaggg ggacgttttt gcctgccttg tccatagctt tgcggaaacc ggcttcgtca
     8641 ttgacgggga cttgcccgac ggcgaggatt tcgtcgccgc gcctcaagcc tgcgcgttct
     8701 gccgcgtcgg aaacccgtac gacgacgagg tgtccgccgc tgctgtcggt atgtgtctga
     8761 agggtaatgc ctgcggattc gaccgagaac gtaccggatt gctgttcggt gtagggggct
     8821 tcatctgttt tggatgatgc gccgatatgc tcggcggcgt tgcccagctt gactttgatt
     8881 gtgatttctt cgcctttgcg ccatacgccg aggctgactt cttttcccgg cgtaatggcg
     8941 ccgaccataa cgggaaggtc gccggaagaa cgtatttctc cgccgtcgag gctgaggacg
     9001 atgtcgcccg cctgcaggcc ggcacgttct gcggggctgc cgggcaggat tttggcaatc
     9061 agtgcgccgc cggctttgtc caaaccgaac gattgtgcca aaccgtagga tacttcttga
     9121 ataatcacgc ccagttgtcc gcgttggact ttgccggtgt ttttcagttg ttcggcaaca
     9181 ttcatggcaa cgtcgatggg gatggcaaag gagatgccca taaatccgcc gctgcggctg
     9241 tatatttgcg aattgatgcc gacgacctgt cctttcaggt tgaacagcgg accgccggag
     9301 ttgcccggat tgatggcaac gtcggtttgg atgaagggtg tgtagctttc gttgggcagg
     9361 cttctgcctt tggcggacac gatgccggcg gtcacgctgt tgtcgaagcc gaagggcgcg
     9421 ccgatggcgg cgacccattc gcccggtttc aaatctttgg gattgccgat tttgacgacg
     9481 ggcagctctt ccgttgcgtc gattttcaga agggcgacat cggattggac atccgaaccg
     9541 atgagtttgg cggtatattc gcgcttgtcg ttgagcagga ctttgatact gcccatgccg
     9601 gtaacgacgt gcgtattggt cagaatatag ccgtctttgc tgatgatgaa gcccgaaccg
     9661 aagttcaatc cgccgtcatc tgcttcttct tgggggattt cgggcatgtt cgggacgagg
     9721 cgtttgaaaa attcgtagaa cgggtcgctg tcggcaagcg ggtcggaatc ggtttcggca
     9781 ttgccgctgc cgttttgggt gcgcggggcg ggggctgcct gaatattgac gactgccggg
     9841 ccttcgcttt gaaccagttg gacaaagtcg ggcagcagca tactgacgct gccgtcgtct
     9901 ttggtgtgtt cgatgcgttc tacgaaagat gcttcttttt tgtccgcacc gaaaaagctg
     9961 ccggctttgt cgcagcctgc cagcgaggcg gcacacagtg ctgccaaagc gaggtattgg
    10021 tattttttga acacgttttg tcctttgtcg gatgccggta ccggctttaa tgccgtctga
    10081 agcgcatttt gtcggcttca gacggcatag gttgaaattc tacaacgtcc gtccgaattt
    10141 tcaagcgttt cattttgaag ggcggcggcg gtcaggcttt ggcgggatat tcgcacaaat
    10201 cgttgatgat gcaggtttgg cattgcggtt tgagtgcctt gcaggtgtag cgtccgtgca
    10261 aaatcagcca gtggtgcgcg tccatcagaa attctttagg aatgaagcgc atcagtttgt
    10321 cttcgacttc gcgcacatct ttcccggggg cgattttggt tcggttggac acgcggaaaa
    10381 tatgcgtatc gaccgccata acggggtgtc cgaacgccgt gttcaatacc acgtttgccg
    10441 ttttgcgccc cacgccaggc aacgattcca aagcctcgcg gtcttccggc acttcgccct
    10501 tgtatttttc caacaggatg cggcaggtct gcatgatgtg cttggacttg gttttataca
    10561 gcccgatggt tttcgtatat tccatcacgc cgtccaaacc caaatccagc atcgcctgcg
    10621 gcgtatcggc aaccggaaac agcttcgccg tcgctttgtt tacgccgacg tcggttgcct
    10681 gtgccgacag taaaacggcg attaaaagct cgaaagggga gttgaagttg agttctgtgg
    10741 tcggatgggg gttggcggcg cggaagcgtt cgaagatttc ttggcggatg tgtctgttca
    10801 tttttttata cggtgggttt gtgtgttcgg cattataacg tatggttcag gcggcgtaat
    10861 attgcattcc ccacagaatg aaggcgtaac gcgccgtttt gccgataacc agcatcagcc
    10921 cgcttgtcca cggattcaac cgcagccagc cggcggcaag cggcagtgcg tcgccgacga
    10981 cgggcagcca ggcaaacgca agcagccaaa taccgaaacg ccgcatcaga ttcagtgttt
    11041 tttcagacgg catttttcgg gagggcagca aacgccccat ccaataggaa accatactgc
    11101 ccaatccgtt ggcaaggccg gcgcacagca acgcgccgta tgcgtgttcg ggaaagcggt
    11161 ggacgaacag ggcaaaggcg gcttcggatg tgccgggcag gagggtggcg gaagtgaagg
    11221 cggaaaaagc gagcgcggcg taggcgtagg cgggcatcat tgcaaacagt ctcaaacagg
    11281 caaaaatcgg cgacggattg ta
//
LOCUS       DAWKQB010000057        11057 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.057, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000057 DAWKQB010000000
VERSION     DAWKQB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.057"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            110..185
                     /locus_tag="WJL14_001123"
     tRNA            110..185
                     /locus_tag="WJL14_001123"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:143..145,aa:Thr,seq:cgt)
     gene            261..1388
                     /locus_tag="WJL14_001124"
     CDS             261..1388
                     /locus_tag="WJL14_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ7656199.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            1569..2126
                     /gene="frr"
                     /locus_tag="WJL14_001125"
     CDS             1569..2126
                     /gene="frr"
                     /locus_tag="WJL14_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ7656200.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            2182..2928
                     /locus_tag="WJL14_001126"
     CDS             2182..2928
                     /locus_tag="WJL14_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ7656201.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            2931..3728
                     /locus_tag="WJL14_001127"
     CDS             2931..3728
                     /locus_tag="WJL14_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ7656202.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMSGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GSWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADVCAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            3733..4968
                     /locus_tag="WJL14_001128"
     CDS             3733..4968
                     /locus_tag="WJL14_001128"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ7656203.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFHPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANETAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDAQTRAQARAFIGTLR"
     gene            5003..6343
                     /gene="rseP"
                     /locus_tag="WJL14_001129"
     CDS             5003..6343
                     /gene="rseP"
                     /locus_tag="WJL14_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ7656204.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGGA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGAPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            6401..8794
                     /gene="bamA"
                     /locus_tag="WJL14_001130"
     CDS             6401..8794
                     /gene="bamA"
                     /locus_tag="WJL14_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ7656205.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTEVLGAIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            8860..9360
                     /locus_tag="WJL14_001131"
     CDS             8860..9360
                     /locus_tag="WJL14_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ7656206.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLKDAKKAQAEEKWCGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            9393..10436
                     /gene="lpxD"
                     /locus_tag="WJL14_001132"
     CDS             9393..10436
                     /gene="lpxD"
                     /locus_tag="WJL14_001132"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ7656207.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDTSVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEESATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDGKDTQ"
     gene            10472..10921
                     /gene="fabZ"
                     /locus_tag="WJL14_001133"
     CDS             10472..10921
                     /gene="fabZ"
                     /locus_tag="WJL14_001133"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ7656208.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
BASE COUNT         2719 a         3439 c         2844 g         2055 t
ORIGIN      
        1 gcgaggcaac gctgtactgg tttttgttaa ttcactatac cgcgcactgc cttgccgccc
       61 gccagaaaag ttgcacaaac aaccgttcat atatatcata acgaaaaacg ccggtgtagc
      121 tcagtcggta gagcagcgca ttcgtaacgc gaaggtcggg ggttcgattc ccttctccgg
      181 caccaatacc aagcacagac cctcccttcc tcgggaagcc tgtgcttttt cacatttccg
      241 cttcagacgg cacaaccgat atgaacacct cgcaacgcaa ccgcctcgtc agccgctggc
      301 tcaactccta cgaacgctac cgctaccgcc gcctcatcca cgccgtccgg ctcggcgggg
      361 ccgtcctgtt cgccaccgca ctcgcccggc tgctccacct ccaacacggc gaatggatag
      421 gcatgaccgt cttcgtcgtc ctcggcatgc tccagtttca aggggcgatt tactccaagg
      481 cggtggaacg tatgctcggc acggtcatcg ggctgggcgc gggtttgggc gttttatggc
      541 tgaaccagca ttatttccac ggcaacctcc tcttctacct caccgtcggc acggcaagcg
      601 cactggccgg ctgggcggcg gtcggcaaaa acggctacgt ccctatgctg gcggggctga
      661 cgatgtgcat gctcatcggc gacaacggca gcgaatggtt cgacagcggc ctgatgcgcg
      721 cgatgaacgt cctcatcggc gcggccatcg ccatcgccgc cgccaaactg ctgccgctga
      781 aatccacact gatgtggcgt ttcatgcttg ccgacaacct gaccgactgc agcaaaatga
      841 ttgccgaaat cagcaacggc aggcgcatga cccgcgaacg cctcgaagag aacatggcga
      901 aaatgcgcca aatcaacgca cgcatggtca aaagccgcag ccacctcgcc gccacatcgg
      961 gcgaaagccg catcagcccc gccatgatgg aagccatgca gcacgcccac cgcaaaatcg
     1021 tcaacaccac cgagctgctc ctgaccaccg ccgccaagct gcaatctccc aaactcaacg
     1081 gcagcgaaat ccggctgctt gaccgccact tcacactgct ccaaaccgac ctgcaacaaa
     1141 ccgtcgccct tatcaacggc agacacgccc gccgcatccg catcgatacc gccatcaacc
     1201 ccgaactgga agccctcgcc gaacacctcc actaccaatg gcagggcttc ctctggctca
     1261 gcaccaatat gcgtcaggaa atttccgccc tcgtcatcct gctgcaacgc acccgccgca
     1321 aatggctgga tgcccacgaa cgccaacacc tgcgccaaag cctgcttgaa acacgggaac
     1381 acggctgacg gtcggacacg atgccgtctg aaacgcctgc cgcttcagac ggcatctcca
     1441 atccgcctgc cttgtcgtca tgcccgaata acggtactat tcgcagttaa cagttttcat
     1501 gccgtctgaa aggtttgaag cgcgtttcag acggctgccc gcctacctta tcttaccgaa
     1561 agaacaatat gatcaacgat attcaaaaaa cagccgaagg caaaatgcag cgttcggtcg
     1621 aagtactgaa agaaaatctg gcgaaagtgc gtaccggccg cgcgcatacc ggcctgctcg
     1681 accaagtgga agtcgaatac tggggcagca tggtccccgt cagccaagtt gccaacgtaa
     1741 cgcttctgga cgcgcgcacc atcggcgtga aaccgtttga gggcaatatg gcggccaaag
     1801 tcgagaaagc catccgcgat tcaaacttgg ggctgaaccc ggcagctgtc ggcgacctga
     1861 tccgcgtgcc gatgcccatg ctgaccgagg aacgccgcaa agacctgatt aaagtcgtac
     1921 gcggcgaagc ggaagaaggc cgcgtctcca tccgcaacgt gcgccgcgat gccaacgacc
     1981 acatcaaaaa actcctcaaa gacaaagaaa tttccgaaga cgaggcacgt cgcggcgaag
     2041 aagcggttca aaaactgacc gacaaataca ttaccgaagc cgacaaactc ctgactgcca
     2101 aagaagaaga tttgatggca atttaacctg cacggttcgg cgttcagacg gcatttgaat
     2161 gccgaaccgc gaaaggcaga catgaaaagc agcacgcagg ccgttttgga acacaccgcc
     2221 attcccaagc atatcgccgt gattatggac ggcaacggcc gttgggcgaa aaaacgtttc
     2281 ctcccacgca taatgggaca caaacgcggt ttggacgcat tggaaaatat ggtgaagcat
     2341 tgcgccaaac tgggtgtgca atatctgacc gtgtttgcct tttcaaccga aaactggcgc
     2401 cgccccgaag acgaagtttc gttcctgatg gggctgtttt tacaggcttt gcaaaaacag
     2461 gtacgccgtc tgcacgaaaa caatatgcgc ctgaaaatac tgggcagccg cgaacgcttc
     2521 agccgtcaaa tcctgaaggg catcgaagaa gcggaggcgt tgacagcaaa caataccggt
     2581 ctgaccctga gcattgccgc cgattacggc ggcagatggg atattttgca ggcggcaaac
     2641 aaactgattg ccgaaggcgt atccgagatt acggaagaca cgctggcgaa acacttgatg
     2701 ctgggcgatg caccggaacc ggatttgttc atccgcaccg gcggcgaaac gcgcatcagc
     2761 aatttcctgc tctggcagat ggcatatgca gaactgtatt tcaccgatat tttgtggccc
     2821 gattttgacg gcaaggcttt ggacgatgcc gtcgcttcgt tccaaaaacg cgaacggcgg
     2881 ttcggacgca cctccgagca actgcctatc gaacagcaaa ggaactgaat atgctgaaac
     2941 aacgggtgat aactgctatg tggctgctgc cgctgatgct gggcatgctg ttttacgcgc
     3001 cgcaatggtt gtgggctgca ttttgcggac tgattgccct gattgccttg tgggaatatg
     3061 cccgtatgag cggcttgtgc aaaatcaaaa ccaaccatta cctcgccgca actttggttt
     3121 tcggcgtggt tgcctatgcg ggcggctgga tgctgcctaa tttggtttgg tatgttgttt
     3181 tggcattttg gctcgccatc atgcctttat ggttgagatt caaatggagg ctcaacggca
     3241 gttggcaggt ttatgccgtc ggctggcttc tggttatgcc gttttggttc gcgctcgtat
     3301 ccctgcgccc gcaccccgat gatgccctgc cgctgctcgc cgtgatgggt ttggtgtggg
     3361 ttgccgacgt ttgcgcgtat ttcagcggca aggcgttcgg caaacacaaa atcgcgccgg
     3421 caatcagccc cggcaaaagc tgggaaggtg caatcggcgg cgcggtttgc gtggcagtgt
     3481 acatgaccgc cgtacgaagt gccggctggc tggcattcga tacaggctgg ttcgataccg
     3541 tgttaatcgg tttggtgctg accgttgtca gcgtatgcgg cgaccttttg gaaagctggc
     3601 tcaagcgcgc ggcaggcatc aaagacagca gcaagctgct gcccggacac ggcggcgtgt
     3661 tcgaccgtac cgacagcctg attgccgtta tcagcgtcta tgcagcgatg atgtcggttt
     3721 taaattgatt ctatgccgtc tgaaaacgct tcagacggca tccggtataa agttatcccc
     3781 attatgacac cacaagtcct gaccatatta ggcagtaccg gcagcatagg cgaaagcacg
     3841 ctggacgttg tctcccgcca ccctgaaaaa ttccgcgtat tcgcgctggc agggcataag
     3901 caggtcgaga aattggcggc tcaatgtcaa acgttccacc ccgaatatgc cgtcgttgcc
     3961 gatgcggaac acgccgcccg gcttgaagcc ctgttgaaac gcgacggcac ggcgactcag
     4021 gttttacacg gcgcgcaggc attggttgac gttgcctctg ccgacgaagt cagcggtgtc
     4081 atgtgcgcca tcgtcggggc ggtggggctg ccttccgcgc tcgcagcggc gcaaaaaggc
     4141 aaaaccattt atctggcgaa caaagagacg ctggtggttt ccggcgcgtt gtttatggaa
     4201 accgcccgtg caaacggcgc ggcagtgctg cccgtcgaca gcgaacacaa cgccgttttc
     4261 caagttttgc cgcgcgatta cgccggccgt ctgaacgaac acggcatcgc ttcgattatc
     4321 ctgaccgctt ccggcggccc gtttctgacc gccgatttaa acacgttcga cagcattacg
     4381 cccgaccaag cggtcaaaca ccccaattgg cgtatgggac gcaaaatctc cgtcgattcc
     4441 gccaccatga tgaacaaagg tttggagctg attgaagcgc attggctgtt caactgtccg
     4501 cccgacaaac tcgaagtcgt catccatccg caatccgtga tacacagcat ggtgcgctac
     4561 cgcgacggct ccgtgttggc gcaactgggc aatcccgata tgcgaacgcc catcgcttat
     4621 tgtttgggtt tgcccgagcg catcgattcg ggtgtcggcg acctggattt cgacgcattg
     4681 tccgcgctga ccttccaaaa gcccgacttt gaccgcttcc cctgcctgaa gctcgcctat
     4741 gaagccatga acgcaggcgg agccgcgccc tgcgtattga acgccgccaa cgaaaccgcc
     4801 gtcgccgcct ttttggacgg acagattaag tttaccgaca ttgccaaaac cgtcgcccat
     4861 tgtctcgcac aagacttttc agacggcata ggcgatatag gggggctctt ggcgcaagat
     4921 gcccagacac gcgcacaagc gcgggcattt atcggcacac tgcgctgatg ccgtctgaac
     4981 accgttatca aaggaaaacc atttgcacac ccttctagct tttatcttcg ccatcctgat
     5041 tttggtcagc ctgcacgaat tcggacacta catcgtcgcc agattgtgtg gcgtcaaagt
     5101 cgtacgcttt tccgtcggct tcggcaaacc gtttttcacc cgaaaacgcg gcgacaccga
     5161 atggtgcctc gccccgattc cgttgggcgg ttacgtcaaa atggtcgaca cgcgcgaagg
     5221 cgaagtatca gaagccgatt taccctacgc ttttgacaaa caacaccccg ccaaacgcat
     5281 cgccatcgtc gccgccggtc cgctgaccaa cctcgcactg gcggttttgc tgtacggact
     5341 gagcttttcc ttcggcgtaa ccgaactgcg cccctacgtc ggtacagtcg aacccgacac
     5401 cattgccgcc cgcgccggct tccaaagcgg cgacaaaata caatccgtca acggcacacc
     5461 cgttgcagat tggggcggcg cgcaaaccga aatcgtcctc aacctcgaag ccggcaaagt
     5521 cgccgtcggc gttcagacgg catcgggcgc gcaaaccgtc cgtaccatcg atgccgcagg
     5581 cgcgccggaa gccggtaaaa tcgcaaaaaa ccaaggctac atcggactga tgccctttaa
     5641 aatcacaacc gttgccggcg gcgtggaaaa aggcagcccc gccgaaaaag caggcctgaa
     5701 accgggcgac aggctgactg ccgccgacgg caaacccatc gcctcatggc aggaatgggc
     5761 aaacctgacc cgccaaagcc ccggcaaaaa aatcaccctg acctacgaac gcgccggaca
     5821 aacccatacc gccgacatcc gccccgatac tgtcgaacag cccgaccaca ccctgatcgg
     5881 gcgcgtcggc ctccgtccgc agccggacag ggcgtgggat gcgcaaatcc gccgcagcta
     5941 ccgtccgtct gttgtccgcg cattcggcat gggctgggaa aaaaccgttt cccactcgtg
     6001 gacaaccctc aaatttttcg gcaaactaat cagcggcaac gcctccgtca gccatatttc
     6061 cggtccgctg accattgccg atattgccgg acagtccgcc gaactcggct tgcaaagtta
     6121 tttggaattt ttggcactgg tcagcatcag cctcggcgtg ctgaacctgc tgcccgtccc
     6181 cgttttggac ggcggccacc tcgtgtttta tactgccgaa tggatacgcg gcaaaccttt
     6241 gggcgaacgc gtccaaaaca tcggtttgcg cttcgggctc gccctcatga tgctgatgat
     6301 ggcggtcgct ttcttcaacg acgttacccg gctgctcggt tagattttac gtttcggaat
     6361 gccgtctgaa accgcattcc gcaccactaa ggaacttacg atgaaactga aacagattgc
     6421 ttccgcactg atgatgttgg gcatatcgcc tttggcattt gccgacttca ctatccaaga
     6481 catccgcgtc gaaggcttgc agcgtaccga gccgagcacc gtattcaact acctgcccgt
     6541 caaagtcggc gacacctaca acgacacaca cggcagtgcc atcatcaaaa gcctgtacgc
     6601 caccggtttc tttgacgacg tacgcgtcga aactgcggac gggcagctcc tgctgaccgt
     6661 tatcgaacgc cccaccatcg gctcgctcaa catcaccggc gccaaaatgc tgcaaaacga
     6721 cgccatcaag aaaaacctcg aatcgttcgg gctggcgcag tcgcaatact ttaatcaggc
     6781 gacactcaat caggcagtcg ccggcctgaa agaagaatac ctcggacgcg gcaaactcaa
     6841 tatccaaatc acgcccaaag taaccaaact cgcccgcaac cgcgtcgaca tcgacatcac
     6901 gattgacgag ggcaaatccg ccaaaatcac cgacatcgaa tttgaaggca accaagtcta
     6961 ttccgaccgc aaactgatgc ggcagatgtc gctgaccgaa ggcggcattt ggacatggct
     7021 gacacgaagc aaccaattta acgagcagaa atttgcccaa gacatggaaa aagtaaccga
     7081 cttctaccag aacaacggct acttcgattt ccgcatcctc gataccgaca tccaaaccaa
     7141 cgaagacaaa accaagcaga ccatcaaaat caccgtccac gaaggcggac gtttccgttg
     7201 gggcaaagtc tccatcgaag gcgacaccaa cgaagtcccc aaagccgaac tggaaaaact
     7261 gctgacgatg aagcccggaa aatggtacga acgccagcag atgaccgaag ttttgggcgc
     7321 catccaaaac cgcatgggct cggcaggcta cgcatacagc gaaatcagcg tacagccgct
     7381 gccaaacgcc gaaaccaaaa ccgtcgattt cgtcctgcac atcgaaccgg gccggaaaat
     7441 ctacgtcaac gaaatccaca tcaccggcaa caacaaaact cgcgacgaag tcgtgcgccg
     7501 cgaattgcgc caaatggaat ccgcgcctta cgacacctcc aagctgcaac gctccaaaga
     7561 gcgcgtcgag cttttgggct acttcgacaa cgtacagttt gatgccgtcc cgcttgccgg
     7621 cacacccgac aaagtcgatt tgaacatgag cctgaccgaa cgttccaccg gctcgctcga
     7681 cttgagcgcg ggctgggtac aggataccgg cctggtcatg tccgcaggcg tttcccaaga
     7741 caacctgttc ggtacgggca agtcggccgc cctgcgcgcc tcacgaagca aaaccacgct
     7801 caacggctcg ctgtcgttta ccgacccgta cttcacggca gacggggtca gcctgggcta
     7861 cgatgtttac ggaaaagcct tcgacccgcg caaagcatcg accagcatca aacaatataa
     7921 aaccaccacg gcaggcgcag gcatccgcat gagcgtgcct gttaccgaat acgaccgcgt
     7981 gaatttcggt ttggtggcag aacacctgac cgtcaacacc tacaacaaag cgcccaaaca
     8041 ctatgccgac tttatcaaga aatacggcaa aaccgacggc acagacggca gcttcaaagg
     8101 ctggctgtac aaaggtaccg tcggctgggg gcgcaacaaa accgacagcg cgttatggcc
     8161 gacgcgcggc tacctgacgg gcgtgaacgc cgaaatcgcc ctgcccggca gcaaactgca
     8221 atactactcc gccacccaca accaaacctg gttcttcccc ttaagcaaaa ccttcacgct
     8281 gatgctcggc ggcgaagtcg gcattgcggg cggctacggc agaaccaaag aaatcccctt
     8341 ctttgaaaac ttctacggcg gcggcctggg ttcggtgcgc ggatacgaaa gcggcacgct
     8401 cggtccgaaa gtgtatgacg aatacggcga aaaaatcagc tacggcggca acaaaaaagc
     8461 caacgtctcc gccgagctgc tcttcccgat gcccggcgcg aaagacgcgc gcaccgtccg
     8521 cctgagcctg tttgccgacg caggcagcgt gtgggacggc aaaacctacg acgacaacag
     8581 cagttccgcg accggcggca gggttcaaaa catttacggc gccggcaata cccataaatc
     8641 cacctttacc aacgaattgc gctattccgc cggcggcgcg gttacctggc tctcgccttt
     8701 aggcccgatg aaattcagct acgcctaccc gctgaagaaa aaaccggaag acgaaatcca
     8761 acgcttccaa ttccaactcg gcacgacgtt ctaatcccgc aaatgccgtc tgaagccctt
     8821 cagacggcat ttcgcggcaa cattcgaagg agttttacca tgacccgttt gacccgcgcg
     8881 tttgccgcgg ctctgatcgg tttgtgctgc accgcaggcg cgcacgccga caccttccaa
     8941 aaaatcggct ttatcaacac cgagcgcatc tacctcgaat ccaagcaggc gcgcaagatt
     9001 caaaaaacgc tggacagcga attttccgcc cgccaggacg aattgcaaaa actgcaacgc
     9061 gaaggtctgg atttggaaag gcagcttgcc gaaggcaaac tcaaagacgc aaaaaaggcg
     9121 caagccgaag aaaaatggtg cgggctggtc gcagcgttcc gcaaaaaaca ggcgcagttt
     9181 gaagaagact acaacctccg ccgcaacgaa gagtttgcct ccctccagca aaacgccaac
     9241 cgcgtcatcg tcaaaatcgc caaacaggaa ggttacgatg tcattttgca ggacgtgatt
     9301 tacgtcaaca cccaatacga cgttaccgac agcgtcatta aagaaatgaa cgcccgctga
     9361 ccctttcaga cggcataccg aacaggaaaa ccatgattcc ggccacctac accctgtccc
     9421 aaatcaccgc gcggctcggc ggcgaatggc gcggcgaaga cacttctgtt accgccgtgc
     9481 gcccgctcgc agacgcgcag gcggaacaca tcagcttcct cgccaacccg aaatacaaag
     9541 ccgaagtcca cgacagcagc gcgggtgcgg tcatcgtttc cgccaaagcg gcagacggat
     9601 ttgaagggcg caacctgatt gtcgccgacg acccctatct ctatttcgcc aaagtcgcgc
     9661 gcctattttc acccgtcgtc aaagcgcgcg gcggcatcca tccgaccgcc gtcgtcgaag
     9721 agagtgccac cgttcccgca agctgcgaaa tcggcgcgaa cgcctacatc ggcgcaaaca
     9781 ccgtgctcgg cgaaggctgc cgcatcttgg caaacgccgt cgtccaacac gattgccgtt
     9841 taggtgatga agttgtttta catcctaacg ccgtcgttta ttacggctgc acactgggca
     9901 gacgcgttga aatccacagc ggcgcggtca tcggcgcgga cggattcgga ctcgccttcg
     9961 ccggcgattc gtggtttaaa atcccgcaaa ctggcgcggt aacgctgggc gacgacgtgg
    10021 aaatcggctc gaacaccaac atcgaccggg gcgcgatgag cgacaccacc gtcggcaacg
    10081 gcaccaaaat cgacaaccaa gtccaaatcg gacacaactg caaaatcggc tcgcacaccg
    10141 tcatcgccgc caaaaccggc atctcaggca gcgtcaccat aggcagctac tgcatcatcg
    10201 gcggcggcgt cggcacggtc ggacacatcg aaatcgccga caaaaccacc atcggcggcg
    10261 gcacgtccgt cacccacagc attaccgaaa gcggcaaaca cctcgccggc atcttcccga
    10321 tgtccaccca taaagaatgg gcgcgcaacg ccgtttacat ccaccgctta agcgaaatga
    10381 acaaacgcct caaaacgctg gaacagcagc tttcagacgg caaagacacg caataaatcc
    10441 aaaccgactt tattcaagga atacgacaga catggacgtt caactcccca tcgaagccaa
    10501 agacatccaa aaactcatcc cccaccgcta cccgtttctc cagctcgacc gcatcaccgc
    10561 cttcgagccg atgaaaaccc taaccgccat caaaaacgta accataaacg aaccccagtt
    10621 ccaaggccac tttcccgacc tgcccgtgat gcccggcgta ctcatcatcg aagccatggc
    10681 gcaagcctgc ggcacactcg ccatcctcag cgaaggcggg cgcaaggaaa acgaattctt
    10741 cttcttcgcc ggcatagacg aagcccgttt caaacgccaa gtcatccccg gcgaccaact
    10801 cgtctttgaa gtcgagctgc tgaccagccg gcgcggcatc ggcaaattca acgccgttgc
    10861 caaagtggac gggcaagtcg ccgtcgaagc cgtgattatg tgcgccaaac gcgtggtttg
    10921 agcgttcgga aaaaggtcgt ctgaaagttt cagacgacct aataaaaaga aaacccaccg
    10981 gtttaggttt attcaaaccc agggacgcag tggggattag tttcacctaa ctgtatcaaa
    11041 tatagtttga aacgtca
//
LOCUS       DAWKQB010000058        10748 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.058, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000058 DAWKQB010000000
VERSION     DAWKQB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.058"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            103..285
                     /locus_tag="WJL14_001134"
     CDS             103..285
                     /locus_tag="WJL14_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656209.1"
                     /translation="MFWYVIGFCAFVVALLSLWVNAGAFGMQEDDTPQSDYERRLGLG
                     AKLKNKNTPKSGEKRQ"
     gene            complement(335..1336)
                     /locus_tag="WJL14_001135"
     CDS             complement(335..1336)
                     /locus_tag="WJL14_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7656210.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRATWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAD
                     KIDLNFGCPAPTVNRHKGGAILLKEPELIFHIVKTLRERLPAHIPLTAKMRLGYEDKS
                     PALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVDIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1413..5246)
                     /locus_tag="WJL14_001136"
     CDS             complement(1413..5246)
                     /locus_tag="WJL14_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ7656211.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRHQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEFVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLTYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLNGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5507..5603
                     /gene="ffs"
                     /locus_tag="WJL14_001137"
     ncRNA           5507..5603
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WJL14_001137"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5771..7462
                     /gene="dld"
                     /locus_tag="WJL14_001138"
     CDS             5771..7462
                     /gene="dld"
                     /locus_tag="WJL14_001138"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ7656212.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGRALAVV
                     RPGTILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKIGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7594..7701
                     /locus_tag="WJL14_001139"
                     /pseudo
     CDS             7594..7701
                     /locus_tag="WJL14_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7757..8128
                     /locus_tag="WJL14_001140"
     CDS             7757..8128
                     /locus_tag="WJL14_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ7656213.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8283..8831
                     /locus_tag="WJL14_001141"
     CDS             8283..8831
                     /locus_tag="WJL14_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ7656214.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8966..10057
                     /locus_tag="WJL14_001142"
     CDS             8966..10057
                     /locus_tag="WJL14_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ7656215.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10514..10684
                     /locus_tag="WJL14_001143"
     CDS             10514..10684
                     /locus_tag="WJL14_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ7656216.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIAPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT         2487 a         2949 c         2921 g         2391 t
ORIGIN      
        1 gccgccttgt ccttatttaa atttaattca ctatatttgc ccgtgcgttt gtgataaatt
       61 cggcactgta tttgatatga ttttaacaaa tcgggagggg caatgttttg gtatgtaatc
      121 ggcttttgcg cctttgtcgt cgcgctgctg tcgctgtggg tcaatgccgg cgcgttcggt
      181 atgcaggaag acgatacccc gcaatcggat tatgaaaggc gtttgggttt gggggcgaaa
      241 ctgaaaaaca aaaatacgcc gaaatcaggg gaaaaacggc aataaagaca gcaaatgctt
      301 tcagccggca tttgctttga agcatgggag acagtcaaac atttatctca tgctcaaatg
      361 cgttcaggat tcggcggact tcatccgcat ccttaaccgt tcggacgcgg tcaaacagcg
      421 tttgcgccgg gtcgaacgtt tttttcatca tgcccaacca ttgcttaaga cgggcgatcg
      481 ggtatttatt gtttgcctct tttgtcaggc acagctcgaa aaactgccgt atccatttag
      541 aaacttcggc aaaatccgtg tctttgaccg gcccgccgtt ctcgtattgc ttgatttgcc
      601 gcgccaaatc ggggcggatg accgcgccgc gccccagcat gacgctgttg cagccgctga
      661 ttgttttgat gccgatatag tcctgcaggc tgaagacatc gccgttggcg gtaactggga
      721 tatcgacgct gtctcggatt ttccttatcc attcccaatg cgccggcggt tcgtaaccct
      781 cggctttggt acgcgcgtgt acggtcagtc cgcacgcgcc cccttcggca atcgcacagg
      841 cgcattccaa agcagggctt ttgtcttcat agccgagccg catttttgcg gtgagcggaa
      901 tatgtgcggg caaacgttcg cgcagcgttt tgacgatgtg gaatatcagt tccggttctt
      961 tcagaagaat tgcgccgcct ttgtgcctgt tgacggtggg cgcggggcag ccgaagttca
     1021 aatcgatttt gtccgcaccg aagcggacgg cttccagcgc attcgccgcc atattgtccg
     1081 catcgctgcc cagaagttgg acggtgcagg gcgtgccgga aaacgttttg tttccgttgg
     1141 cgatttcggg gacgtatttt aaccatgtgg cgcgggaatg cacggtatgg gtaatgcgta
     1201 caaattcgct gacgcattcg tcgtagccgc caatacgcgt cagcaggtcg cgcatcacgt
     1261 catcgaccag accctgcata ggggcaagga tgattcgggt tttttgtttg ggttcggttg
     1321 tttgtccgtc aatcatgatt cgggtaggta tttgattcaa acaatgccgt ctgaaggctt
     1381 tcggcgttca gacggcattt ccgtgtccgt tgttacagca atactttttc cacgccgccg
     1441 ttgttggctt ttttcacaaa ctcgtctagc cagttttcgc cgaggatgtg tttcgccatt
     1501 tcgatgacga tgtagtcggc aggcatattg ttgtcgtcgg tgtagcggct caagccttgc
     1561 aggcaggctg ggcaggaagt aagcattttg acgggttcac cctgcggcag ctctttgagg
     1621 tttttctcga tttcctcttg tttgcggaat ttgacttggg tggcgatatc gggtcgtttg
     1681 acggcgaaca tgccagattc gccgcagcag cggtcgctta agacgacttt ctgccccatc
     1741 aggctgctgg ccatttgggt ggcgttcatg gttttgatgg gcgtgtggca aggatcgtgg
     1801 tagaggtatt gctgaccttt tacgccgttt agtttcacgc ctttttcgag cagatattcg
     1861 tggatgtcga tgatgcggca gccagggaag atttcttcaa agcggtattt ttcgagctgg
     1921 tcgtaacaag tgccgcaact gacgacgacg gttttgatgt cgaggtagtt gagggtgttc
     1981 gccattcgat ggaaggcgac gcggttgttg gtgctcattt cttcggcttt tgccttgttg
     2041 ccgcccgcgt cttgcggata gccgcaacac atatagcccg gcggcaggac ggtttgtacg
     2101 ccgacgtgcc agagcatggc ttgaacggca agtccgattt ggctgaacag gcgttccgaa
     2161 ccgcaacccg ggaagtagaa cacggcttcg gcatcttcgg gcgcggcggg gttgcggatg
     2221 atggggatgc ttttgccgtc ttcgatgccc aataaggagc gcggtgtttt agcgggtacg
     2281 cttttgggca gtgggcggtt gatgaaatgg ataacctgtt ctttaatcgg ggctttgccg
     2341 acggttgcct tgggttcggc tttttgcttt ttcgtgccga tcggaagaag cttgccgatt
     2401 ttgtaggcaa agttttgcgc ggggaagccg gtttgtatca tggcggcgcg aagggctttg
     2461 atggttttcg ggccggtggc gttcaaaaac gccataccca tagctgcggc aggcgcaaat
     2521 cgtttgtggc cggaatcggc aaggtagttg cggacggcta cggttacgtc gccgaagtcg
     2581 atgttgacgg ggcagggttt gacgcagcgg tggcacacgg tgcagtggtc gccgatgtcc
     2641 atgagttctt caaagtgttt gatggaaacg ccgcgacggg tttgttcttc gtataagaag
     2701 gcctcggtca gcaaacccac gccgaggatt ttgttgcggg ggctgtacag caggttggca
     2761 cgcggaacgt gggtggagca gacgggtttg catttgccgc agcgcaggca gtctttgacg
     2821 gaatcggcaa tcgtgccgag gtctgatttt tccataatca gcgattccgc gcccaaaagc
     2881 tcgaaagacg gcgtgtaggc gttgcgtaag tccgagcctt tcatcagttt gtgacggttg
     2941 aaggtgtgtt tgggatcgac ttggtttttg tagtcccaaa acggctgcat ttcttcgtcg
     3001 cttaaaaatt cgagcttggt gatgccgatg ccgtgttcgc ccgaaatcac gcctccgagc
     3061 gaacgggcga ttttcataat gcgttcgact gagcggtatg ccgtctgaag catttcggca
     3121 tcgtctgagt taaccggaat attggtgtga acgttaccgt cgccggcgtg catatgcagg
     3181 gcgacaaaga cgcggctgcg tacggttttt gcgtggattt ttcccaagcc ttggataatt
     3241 ttggtgtcgg ttttgccgct gaagatttca gaaagcggct tcattacgtc cgctttgaca
     3301 gacacgcgca ggcggaaatc gcggaatgcg gtaaagcagc tttcattgtc tttggcttcg
     3361 ggcgcggcgt ggacggctga gccatagcgt gctttatagt cggcaagcgg cgcgtccaag
     3421 ttggcaagca gccattccca acgcgctttg acggcggaaa cgtgggcaag ggcgtgtttg
     3481 ccgcgttcgc ccaacagttc ggcggtcggc aggtctgttc ccattttgtc gatggggagt
     3541 ttgcccgaaa gatattgctc caatgcggca cagagtttga gtttgttttg gatggaaagc
     3601 tcgatgttga tgcgttcgat gccgtctgaa tactcgccga gcctttcgag tgggatgacc
     3661 acgtcttcgt taattttaaa ggcgttggtg tgcttggcga tggcggcggt gcggctgcgg
     3721 tcgagccaga aggttttgcg cgcttcgggc gatacggcga taaagccttc gccgtcgcgg
     3781 gcgcgggcaa gttcacagat gtgctcggcg gctgcctcta cggcggcttc gtcgtctgaa
     3841 accacgtctg ccagcaagac cattttcggt cgtcccttgc ccgccgcttt ggtggcgtag
     3901 ccgacggcgc ggacgtaacg ccagtctaaa tgttccaaac ctgccagccg aacgctgtcg
     3961 tgggcgagca ggaaatcgcg gatttcaacg atagaaggcg tggcggtggc gaccgtgccg
     4021 aaaaactcca tacacacggt gcgcgtgtat ttcggcattt tgtgcaacac gaaggcgacg
     4081 ccggtgatga tgccgtctgt accttctttt tgcacgccgg gcaggccgct caagaatttg
     4141 tcggtaacgt ctttgcccaa accgactttg cggaatttgt ggccggggat ttccaagcgt
     4201 tcggtcttaa cgatgttgat gccgtctgaa tccagcgtgt gaacgtcgaa cacggcggtt
     4261 tcttcatcgt ggattttgcc gaaattgtgg cgcacacgtt cgatacgcaa ccattcgccc
     4321 tgcgggttga ccatctgcca gtaggtgagg ttgtccaacg ccgtccccca caacacggct
     4381 tttttaccgc ctgcgttcat cgccacatta ccgcccacgc aggacgcatc ggcggaagtc
     4441 ggatcgacgg cgaacaccaa acctgcctga tgcgcggttt cttccacccg ccgcgtaacc
     4501 acgcccgcgc cgcaccggat aatcggatgc ttgccgtcca atcctgccag ttcgacaaat
     4561 tcgacaccgc gatgcttgtc gagtttttcg gtattgatga ctgcgctcat tgcgtccaaa
     4621 ggaatcgcgc cgccggtata acccgtgccg ccgccgcgcg gaataatgac caaatccagc
     4681 tcgattaagg cgcgcaccaa aggcgcgact tccgcctcag tgtcgggatt gacgacgaca
     4741 aacggatact ccacgcgcca gtcggtcgca tcagtaacgt gcgttacccg cgccaatccg
     4801 tcaaacatga tattgtgcgg tttggtaatt ttgcttaagc gttccaaaat ctgatggcgt
     4861 ttttggctgg tttcatcaaa actgctatca aaacgctcga ctgctttttc tgctgcggca
     4921 accaagacat ccacttgccg attatcgtcg cggcgtttgc ggatttcgtt caacctgtgg
     4981 cgcatttcgc gtaccagcgc ggcgcggcgt ttcggatgtt ccaacaagtc atcgaccaga
     5041 tacggattgc gcacgaccac ccaaatatcg cccaatactt caaacagcat tcgtgctgaa
     5101 cgcccggttt tacgctgtcc gcgcaggtct tgcaggattt gccacgcttc gtcgcccaat
     5161 aatcggatga cgatttcgcg gtcggtgtag gaagtatagt tatagggaat ttcccgaata
     5221 cgctgcgggg cggtagtcgt ggtcatggtg tgtccgtgtt tggcaatttt tatttgaatg
     5281 ccgtctgaaa agtcggtttc aggcgggttt gaaaaggcga taatgtagtt caatttttga
     5341 tatttttcaa tgccgaaaca ggaaataccc ttatagaaac aaaataacgc tttgagaaat
     5401 tgttgacaat attatcaaat ataaatttta ggtggtgttt ccggcgtatg accgtatcaa
     5461 aacggttgag tttttcagaa aacccgttat aatgccgttt ctctgaggcg ggtctccccg
     5521 catggcaaat cggaacaccg ggtcaggggc ggaagccagc agcccactcc ggtgcgccag
     5581 tgccgggggt ttggcccgcc gccctatttg aaacgccgga gcttcgatgt tccggcgttc
     5641 tcgtaccgcg ccgatgccgt ctgaaagccg ttcagacggc attttcttta ctttgccata
     5701 acgtataact acaatgggaa aacaatttca atgtgcatat gcacctgccg acaattttaa
     5761 ggaaactgcc atgagcgcat cacaattact gtcaagactg acccaaaccg taggcgaaaa
     5821 atacatcatc accgaccccg cgaagaccga acaataccgc caaggctacc gcttcggcga
     5881 gggcagggcg ttggcggtgg tgcgccccgg aaccattctg gaaatgtgga aaattctgca
     5941 ggcctgcgtc gaagcggacg tgattgtgat tacgcaggcg gcgaataccg gtttgacggg
     6001 cggctcgacc cccgacggca acgattacga ccgcgacatc gtgattgtga acaccatgcg
     6061 gatgaacatc atccaaacca tcaacaacaa cgaacaagtc gtctgcctgc ccggctcgac
     6121 cctgaaccag cttgaattgc tgctgaaacc tttggggcgc gaaccgcatt cggttatcgg
     6181 ctcttcctgc atcggtgcgt ccgtcttggg cggcgtgtgc aacaactccg gcggcgcatt
     6241 ggtgcagcgc ggcccggcct acaccgaaat ggcactgttc gcccaaatca acgaagaagg
     6301 caggttggaa ctggtcaacc acttgggcat agacttgggc gacacgcccg aagaaatcct
     6361 gaccaacctg caaggtcatc attatcaaaa caaagacatc aaacaagacg cgggcaaagg
     6421 gcacgaccac gcctattgcg aacacgtccg ccaagtggac gaaccgaccg ccgcgcgttt
     6481 caatgccgac cccgcccgcc attacgaagc ctcgggctgc gcgggcaaac tgatggtttt
     6541 tgccgtccgt ttggacacct tcccgcaaga aaaacaaacc gccgtgttct acatcggcac
     6601 gaacgacatc aacgagctga ccgacatccg ccgcgccgcc ttgggcgaat ttgaaagcct
     6661 gcctgtttcc ggcgaataca tccaccgcca cgctttcgac attgccgacg tgtacggcaa
     6721 agacacgttt tacgtcatca aaaaattcgg tacgcaccaa ctgccgaaat tatttgactt
     6781 gaaggcgcgc gtggacaggt tcggcaaaaa agccagcttc ctgcccaaac atttttccga
     6841 caaggccatg cagttcgtca gcaaatacct gcccgaccac ctgcccaaat ccatgcgcga
     6901 ttaccgcgac aaatacgaac accacctgat tctgaaaata ggcgggaaag gcgtggatga
     6961 ggcgcgcgcg ttcttaaaag aatattttgc acaccacagc ggcgcgtttt tcgagtgcaa
     7021 cgccgaagaa acccaagccg cgatgctgca ccgtttcgcc gtcgcctccg ccgccatccg
     7081 ctaccgcgcc gtgcatgatg acgaagtgga agacttggtc gcgctcgaca tcgccctgcg
     7141 ccgcgacgat cgcgactggt ttgaaaaact gccgtcggaa atcgacaata aaatcatcca
     7201 taaactctac tatggacatt tcatgtgcca cgttttccat caggattaca tcatcaaaaa
     7261 aggcaacgac tgcatggcgt tggaacacga aatgctgcat cttttagacc aacgcggcgc
     7321 gcaatatccc gccgaacaca acgtcggtca tttgtatgaa gccaaacccg cgctcaaaca
     7381 gttttaccgc aaacttgacc cgaccaacag cttcaacccg ggcgtaggca aaaccagcaa
     7441 aaagaaaaac tgggcggaat aagcgcgtcc gctttgaagg caggcaatgc cgtctgaagg
     7501 caaaacgccg ttcagacggc attgtcgggt ttgaagttcc ccgtcgggta atcgctttca
     7561 aaccgtatgc cgacacacgg gcgcgcccct cccaaatata gtggattaaa tttaaatcag
     7621 gacaaggcaa cgaagccgca gacagtacag atagtacggc aaggcgaggt aacgccgtac
     7681 tggtttaaat ttaatccact ataaagtctg atcctacctt cccaaagggc agggtttcaa
     7741 ccgaaaagga aacacgatga aaccatacaa aatctacacc catcccgccc tgccgcccca
     7801 agccgtcaaa caaggctggt catggccggg gctcttgttc ggcacgctgt gggcgtgttt
     7861 caaaagaatg tggggtttgg ggctggggct gacgggcgcg atattcgtgc tggctgtgtt
     7921 tgcccaactg gtttacggcg acacgcccgc cacagactcg gcgtttaacg tattgggctt
     7981 ggcggtttcc gtctggttcg gcgcaaaggg caacagcctt tatgcacgcc accttttatc
     8041 gcgcggctat accgaattgc ccgaaacggt tgaggcagcc aatccccaag ccgcattggc
     8101 gcaatatttc gggcgcgggg gccgataagc gcgttttctt ttccatcgtg ccgtctgaaa
     8161 aatgttcaga cggcatttct tataaccttt cgtttgcagc acataaccaa tcagtctttc
     8221 ctattccgtt ccgaaatgca taccatgcgc ccgtacccaa tatttgcgaa caaggaaaga
     8281 aaatggcacg tttaaccgta cacaccctcg aaaccgcccc cgaagccgcc aaagcgcgcg
     8341 tcgaggcggt acttcaaaac aacggcttta tccccaacct tatcggcgta ttatcaaacg
     8401 cccccgaagc cttggcgttt taccaagaag tcggcaagct caacgccgcc aacagcctga
     8461 ccgccggcga agtcgaagta atccagatta ttgccgcccg caccaaccaa tgcggcttct
     8521 gcgtggcagg gcacaccaaa ctcgcaaccc tgaaaaaact cctttccgaa caatccgtca
     8581 aagccgcgcg cgctttggcg gcaggcgaat ttgacgatgc taaactcggc gcgctcgccg
     8641 cctttaccca agccgtaatg gcaaaaaaag gcgcggtatc cgacgaggaa ctcaaagcat
     8701 tttttgatgc gggctacaac cagcagcagg cagtcgaagt cgtgatgggc gtagccttgg
     8761 caactttgtg caactacgtc aacaacctcg gacaaaccga aatcaacccc gaattgcagg
     8821 cttacgcctg atacggcaaa ccgcccgaat atcgggcggt ttttcaaaaa caccccctca
     8881 aacaaaaaca agccgcctaa gcggcaggga agcagccgcg catcaggcgc gcccccgatt
     8941 tccccgcaaa ccttaaggaa caaagatgaa cgcccaaacc ctgattgcca acgttgccga
     9001 attcgtcaaa accaagctca aacccatagt ggacgacatc gaccgcaaag gatactaccc
     9061 cgaagcattt atgcgcgaac tcggcgcaat cggtggattc ggcgcagtcg gtacagaagc
     9121 cgaaggcggc aacggcttgg gtttggcaac gcaaatcgcc gtattgcgcg aaatcggcaa
     9181 agaatgcggc gcaacctctt tcagtgcgtg gtgtcaggcg gcttgcgcgt ggtatctgca
     9241 ccaaacgccc aaccgggccg tcaaagacaa ataccttgcc gacatcctgc aaggcaaagt
     9301 attggcgggt acgggcatgt ccaataccgt caaacacctt gccgacatcg aaaaacacaa
     9361 cctccaagcc gagcgcgtgg agggcggcta cacagtcaac ggcgcgctgc cgtgggtgtc
     9421 caacatcggc gaagaccaca tttgggcgaa taccgcccaa atcggcggcg gctacgtcat
     9481 gttcatcaca ggcggacaat gggaaggcgt aagcctgcaa aactgccccg aattttgcgc
     9541 cctcgaaggc acgcgcacct tcagcctgaa cttcaaagac gtatttatcc ccgacgaaga
     9601 catcatcgcc gcgcccgaac agtttgccga ctacatccaa agcatcaaag caggctttat
     9661 cctcctgcaa atcggcatcg gcgcgggcgt gatagacggc agcctcggca tcatccgcct
     9721 cgccaacgtc gtcaacgccg aagtcaaccg ctatctcgac gacggctacg acagcctcaa
     9781 aacaaggctg gacggcgcgt gggcagaaac cgaacggctc gccggattgg cttggagcgg
     9841 cacgcccgac aaccttacca ccctcaagct gcgcgaagcc gccgccgtac tgacacttgc
     9901 cgccgcccaa tccgccgccc tgcattccgg cgcaaaaggc tacctgatgc ggagtcccgc
     9961 ccaaaggcgc gtccgcgaag caatgtttgt cgccatcgta actcccgcga tcaaacacct
    10021 ccgcaaagaa atagcggcaa tcgaagccgc aaaataaaca ggcggaaaaa accgaaaccc
    10081 ccatcgcgtc attcccgcga aagcgggaat ccagccccca acgtaacagg aatccatcgg
    10141 aaaaaacaga aaccctcaaa ccgtcattcc cgcaaaagcg ggaatccagc ccccctaacg
    10201 caacaggaat tcatcgaaaa aaccgaaacc tccaaaccgt cattcccacg gaagtgggaa
    10261 tccagtaatc gaaaaaccac aggaatctat cgaaaaaaaa ccaaccccaa aaaaaccggg
    10321 cggataccgc accattctcc caaaccctga tttatagcgg atgaacaaaa atcagggcaa
    10381 gacggcgaag ccgcagacag tacaaatagt acgggtgctt cagtacttta gagaatcgtt
    10441 ctcttcgagc taaggcgagg caacgccgta ccggtttttg ttaacccact atacaaaaaa
    10501 caaggaaaca caaatggcgc aatatatgtg cggcccctgc ggctggattt acgatgaaga
    10561 acacggcgac cccgaacacg gcatcgcccc cgggacaaag tttgaagaca tccccgacga
    10621 ctggaaatgc cccgaatgcg gcgtgggcaa agaagatttc tacctgttgg atttcgtgat
    10681 atagcggcag ccgcaccctt agggaaaacc ccgtcatccg gcgttccgcc tgcggcggca
    10741 gggaacac
//
LOCUS       DAWKQB010000059        10704 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.059, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000059 DAWKQB010000000
VERSION     DAWKQB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.059"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(27..653)
                     /locus_tag="WJL14_001144"
     CDS             complement(27..653)
                     /locus_tag="WJL14_001144"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ7656217.1"
                     /translation="MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAVDIIK
                     AQKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVDEAIRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
     gene            complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJL14_001145"
     CDS             complement(712..1203)
                     /gene="greB"
                     /locus_tag="WJL14_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ7656218.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLEAAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            complement(1303..2841)
                     /gene="purF"
                     /locus_tag="WJL14_001146"
     CDS             complement(1303..2841)
                     /gene="purF"
                     /locus_tag="WJL14_001146"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ7656219.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVHGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTIIARQCSDKAKLSPCLFEYVYFARPDSVIDGVF
                     VYQSRLDMGVSLAEKIKRELPMDGIDVVMPIPDTSRPSAMELAVHLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISDA
                     GDEE"
     gene            complement(3050..3547)
                     /locus_tag="WJL14_001147"
     CDS             complement(3050..3547)
                     /locus_tag="WJL14_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ7656220.1"
                     /translation="MNNLPVADLLVSAIIAACIVLSAMRGVIAEAGSMAAWVVAFFFA
                     KLFAAPFADIAFASFQPRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            complement(3540..4538)
                     /gene="ftsN"
                     /locus_tag="WJL14_001148"
     CDS             complement(3540..4538)
                     /gene="ftsN"
                     /locus_tag="WJL14_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ7656221.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            complement(4551..5825)
                     /gene="folC"
                     /locus_tag="WJL14_001149"
     CDS             complement(4551..5825)
                     /gene="folC"
                     /locus_tag="WJL14_001149"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ7656222.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAVNAEPVSDDTITASFER
                     IETARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDNPARNRNALPFPALRGTYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            complement(5856..6302)
                     /locus_tag="WJL14_001150"
     CDS             complement(5856..6302)
                     /locus_tag="WJL14_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ7656223.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITPDMADKESYRRLKVWVLWRQPKKTAETDTSD"
     gene            complement(6307..6666)
                     /locus_tag="WJL14_001151"
     CDS             complement(6307..6666)
                     /locus_tag="WJL14_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656224.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDTEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            complement(6739..7467)
                     /locus_tag="WJL14_001152"
     CDS             complement(6739..7467)
                     /locus_tag="WJL14_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656225.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQGFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            complement(7640..8419)
                     /gene="rsmA"
                     /locus_tag="WJL14_001153"
     CDS             complement(7640..8419)
                     /gene="rsmA"
                     /locus_tag="WJL14_001153"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ7656226.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVVAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8616..9305
                     /locus_tag="WJL14_001154"
     CDS             8616..9305
                     /locus_tag="WJL14_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ7656227.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPDCRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            9490..10692
                     /gene="trpB"
                     /locus_tag="WJL14_001155"
     CDS             9490..10692
                     /gene="trpB"
                     /locus_tag="WJL14_001155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ7656228.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
BASE COUNT         2183 a         2751 c         3101 g         2669 t
ORIGIN      
        1 tcattccggc ggcaattttt gttgctttaa cgggataggc ggttggcggt tgcgataaag
       61 gcggcaactt tggctgggtc ttttttgcct ttagacgctt ccacgccgcc ggatacatcg
      121 accgcttccg ctccggtgat gcggatggct tcgtcgacgt tttcaggggt cagcccgccg
      181 gcaagcaccc acggtttgcc cgaatattcc gccaacagcg tccagtcgaa gcggtgtccg
      241 gtgccgccgt attccgaagg atggtaggca tcgaacagca gtgcctgagc gtcggggaag
      301 cggtcggcgg cgtttcggat gtctgatgcc gtctgaacac gaatggcctt gatatagggg
      361 cggtggaact ggcggcagaa tgcgtcgtct tcgtcgccgt ggaattggat gatgtgtatc
      421 ggtacttcgg caaggatgcg gcggatgttt tgcgcgcttt cgttgacgaa aagggcgaca
      481 acgctgacaa acggcggcag tgcggcggtg attttttgtg ctttaatgat gtcgacagcg
      541 cgggggcttt gggggtaaaa aaccagtccc aatgcgtctg cgccggcgtg ggcggcatac
      601 agtgcgtctt ccggtgtggt gatgccgcag attttggtgc ggattttcct cattcggtat
      661 tcctttattt gggaaacggc gcgtttgtgc cgtttcagac ggcattcccg atcagtcgat
      721 tttgatgtat tcgacagaaa ggatttcaat ttcctcacgc ccttccggcg tgtttaaaac
      781 cacttcgtcg ccttcacgcg ctttaatcag acaacgagcc agcggcgaaa tccaagaaat
      841 tttgttttgc gcggtatcga tttcatcgat gccgacgatt ttgacggttt gctcgcgccc
      901 gtcgccgcgc aacagaccga cggtcgcgcc gaaaaacact tggtcggtcg cttcgcgcaa
      961 ttcgggatca acgacgacgg cggcttccaa acgcttggtc aggaaacgga tgcggcggtc
     1021 gatttcgcgc atacggcgtt tgccgtaaag gtagtcgccg ttttcgctgc ggtcgccgtt
     1081 gcctgccgcc cagttgacga tttggacgat ttcggggcgt tctttattaa ccagttgata
     1141 aagctcgtct ttcaatgcct gccatccggc gggcgtaatg tagtttttgg tttcggtact
     1201 catattgtgt gcggatgaaa cgggaaatgt gatgccgata tgggaaatgc cgtctgaaaa
     1261 cccggcgttc ggatttcaga cggcatcgcg gtttgggaag ccttattctt cgtcgcccgc
     1321 atcgctgatg ctgtgttcca tcctgctcgg gtggattttc agaccgccgc agccggattt
     1381 ctcggcagac aggcggtcga ggtaggcatc atcgatgtcg ccggtctgat aaatgccgtt
     1441 gaaacaggac gaatcgaagg attcgatttt cgggttgagt gctttgacga cggcttccaa
     1501 atcgcccaag tcttgaaaca cgatgccgtc cgcgccgatt tcggcggcga tttccgccgc
     1561 gctgcgccca ttggcaatca actcttcgcg cgtgggcata tcgatgccgt atacgttggg
     1621 atagcgcact tcgggcgcgg cggaggcgat atagactttg cgcgcgccag ccgcgcgtac
     1681 catttcgacg atttcacggc tggtcgtacc gcgcacgatg gagtcgtcca ccagcaacac
     1741 gcttttgcct gcaaattcgg tttccatcgg gctgagtttt tggcgcacgg attttttgcg
     1801 cgtcgcctgg ccgggcataa taaaggtgcg gccgatatag cggtttttaa tcaaaccttc
     1861 gcggtaaggc tttttgaggt ggacagcaag ctccatcgcg ctggggcggc tggtgtcggg
     1921 gatgggcatc acgacatcga tgccgtccat gggcagctcg cgtttgattt tttccgccaa
     1981 ggacacgccc atatccaagc gcgactggta aacgaacacg ccgtcaatca cggagtcggg
     2041 acgggcaaaa tagacgtatt caaaaaggca ggggctgagt ttggccttat cgctgcattg
     2101 tcgggcgatg attgtgccgt caaagccgac aaataccgct tcgcccggcc ggatatcgcg
     2161 ttccaaatcg taggcaagcg cgttgaaggc gacggattcg gaggcgacgg cataggattt
     2221 cctgccttcg ctgtcggttt gcgaacccaa taccagcggg cggatgccgt aagggtcgcg
     2281 gaaggcaagc ataccgtagc ccgcaatcat ggcaaccaca ccgtatgcgc cgtgcaccag
     2341 gcggtggact tcggcaacgg cgttgaaaat attgtcggca ttgagccggt gcgggtcggc
     2401 gtttttagag acttcgcggc gcaattcgtg cgcgaatacg ttgagcaaga cttcggaatc
     2461 ggagctggtg ttgacgtggc gcaggtgttt gttgcacacg ttttcataaa gttcggccgt
     2521 gttggtgagg ttgccgttgt gcgccaaaac gatgccgaac ggcgagctga cgtagaaagg
     2581 ctgcgcctcc gcgctgctgc ccgcgttgcc cgcagtagga taacggacgt gggcgatgcc
     2641 ggcgttgccg accaaatcgc gcatattgcg tgtgcggaac acttcgcgca ccatgccttt
     2701 gcctttgtgc atatggaagg taccgccttc cgccgttgca atgcccgccg catcctgccc
     2761 cctgtgctgc aacatctgca aaccgtcgta cagaagctgg ttcacgggtt catgactgac
     2821 caaacctaat acgccgcaca tatcgtcttc tccgattcga ggtttaaggg taaaacggaa
     2881 ttataaagca aacggtggtt ttttgcctga attgttgaca atatttgagt gaaggacaga
     2941 taggtgggtt tatggaggat aagatttaat ctgtgatatg gctgaggaaa ggaaaaaaac
     3001 atttcagacg gcataaaaga gaatgccgtc tgaaatatcc gtatggcaat caatcgtctt
     3061 ccggagtttc cgccgtgccg ccgctatggt tcaacacggc ttcggaaagc gatacgaaaa
     3121 acggcagtgt gtaagattgc cgccattctt cggtatcggg caggtcggtt tttgaagcga
     3181 gcatgaccag cagggtaata atcaaaatgc ctttcaatgc accgaatacg ccgcccaaaa
     3241 tgcggttggc aaagcccaaa ccgaccgccg aaactgcccc ggtcagcagc gagcggagta
     3301 ttttctggat cagacaggca atgacgaata gggaaatgaa cgacagagcc aatgcaaaca
     3361 ggcggggttg gaacgatgca aaggcgatgt cggcgaaggg tgcggcaaag agtttggcga
     3421 aaaaaaaggc aaccacccat gccgccattg agccagcctc cgcaatcacg ccgcgcatcg
     3481 cggatagcac gatgcaggcg gcgatgatgg cggagacgag gaggtcggca acggggaggt
     3541 tattcattcg ttacctgacc ggcgataccg tgtacgcgca atttgttcaa atcgcgttcg
     3601 gcatcccttg cgtttttata gttgcttgat ttgacgcggt aaactttgcc gttgtcggtc
     3661 ataatttcgg tgatggtcga atcgataccc gccgccttca ttttgcgctg gaggcttaag
     3721 gcgcgttctt tttcggcata acctgcctga atggcggctt ttttaccgga tttttccttc
     3781 tcggcggttt tggtcttgtc cgctttgtcg gttttttgtg ccgtttcgtg ttttttgccg
     3841 tccgaacggt ctttttcggc tgtttttttg ctttcagcct tgtcggcttt tttcgcttct
     3901 tttaccgcgc tgtcggattt tgccgtatcc ggtttggttt tttcggcggc agttttcggt
     3961 ttgtcggcaa ctttttcggc ggttttggtt tctttggctt tgggcttggc tttggcagtg
     4021 cgttccgctt tttgcggttt tgtttcggca gtgcgtttcg gtttttcaac cgctaccgta
     4081 tccgtactgt cggcagttgc cggcactttt tcggcagcgc gttgttttgc ctgcttcggt
     4141 gcggttttgg cggtttctgc ctgttgcagt ttctcggatg cttccaaacc tttgatgttg
     4201 ctgtcttcga ggcggtcgtt aatcagcacc agcggcgcgc ctacgttttc aggctcgctg
     4261 atttcgctgt cggcggcaga aggcttgtct tcgcctgcca agtcctgcgg tttgtcggcg
     4321 gcggatttca aggcaggggt ttgtgccgca cctgccgctt tgttttctac gccgcttgtt
     4381 tcgccggcag tctgttcggc agggccggaa ctgagggcgg ctgccagcag gatgcaggag
     4441 gcggcaacca ggcaacttgc cgttacgagg cggcggcggt tgcgccgttt gagttgttcg
     4501 taaccgctca ggacttcgtt ttgtttgttt tcggacatag aagtttcctt ttaaagtacc
     4561 gacatgacat cggcaacggt atgaaatgag ccgaaaacga cgattctgtc gttctcgccc
     4621 gcttttgagg ctgccgcccg gtaagcttcg cggacggcgg cgaatgtctg tatgttttcg
     4681 atgctgtgtt cgtgcagttt gttttgcagg ctctcgagcg tcatgccgcg cggtacgtcc
     4741 aacggtgcga tataccactc gtcaaactgg tctttaacga tttccaacac gccgtctatg
     4801 tctttgtcgg acagcatact gaatacggcg gtgcgttttt gcgcaaacgc caaattaatc
     4861 aggctgcggc gcagggcgcg ggcagcgtgg ggattgtgtc cgacatccaa aacggtcagc
     4921 ggacggccgg gcaggacttg gaagcgtccg ggattttcaa ccagcagcaa tccgcgtttg
     4981 attgcaccga tgtccacagg caatttgtcg ttcaagcatt ccaatacggt caacgtgcag
     5041 gcggcgttgg aaagttggta tgtgccgcgc aatgcgggga agggcagggc attgcggttg
     5101 cgcgcggggt tgtctgaatg ttgcggccgg aagcggtagt tccattggat gttttccatc
     5161 gcgtgaaact cgaaatcgcg ctgcaccatc agcagttttg cacctatggc ttcggcgtgt
     5221 gcgaccaatg atgcgggcgc ggggttttga ccgcagatgg caggtttgcc gctgcggaac
     5281 acgcctgcct tttcaaagcc gacctgttcg accgtatcgc ccaaaaacgc ctgatggtcc
     5341 aaatccacgc tggtaaccac cgcgcaatcg ccgtcaaatg cgttgaccgc gtccaagcgt
     5401 ccgcccaagc cgacttccaa tatcatcacg tcaacctgtt cgcgcatgaa gatgtcgacg
     5461 gccgccaagg cattgaattc aaaataagtc agtgatattt caccgcgcgc ggtttcaatg
     5521 cgttcgaagg aagcggtgat tgtatcgtcc gaaacgggtt cggcgttgac ggcgatgcgt
     5581 tcgttgtaac gcaataaatg cgggctggtc agcgtaccgg ttttgtagcc cgcctgtttg
     5641 taaatctgtg tcaggtaggc acagaccgaa cctttgccgt tggttcccgc gacaacgacg
     5701 acggggcatt gcggctcgag cttcatgcgt tttttcactt cgctcgtgcg ctccaaaccc
     5761 atgtcgatca aaccgccgct gtgggcggtt tccaaatgcg agagccagtc ttgtagtgtt
     5821 ttcatgagtg tttcgttttc aaatgccgtc tgaaatcagt ctgatgtatc ggtttcggcg
     5881 gtttttttcg gttgccgcca aagcacccag actttcagcc tgcggtagga ttctttgtcc
     5941 gccatatcgg gggtgatgca gtggcggacg gttttgccgc cggtgtccca ttgtaagaat
     6001 aaggcataag gcgtaaccat actgctgccc gacagtgccg ccgcctttgc cgttttttct
     6061 ttgccggata cgatttccgc ctgtccgtcg cggtctatgg taatggcggt tatggcatgg
     6121 cggtgtttca gattcgttat cctgagcgag tatgcgtaac ttgccgccaa agccgccaaa
     6181 ccgaaccaca tcatccggcc gtaaaaccag gtcaggcaga cggcaaggga agccgagtgg
     6241 agcgatacgg tcaggatgtt cagaatgcgg gacggcctca atgccgtctg aaaggcgcgc
     6301 acagccttac atcatgttgt cgaacacggg ggtaatgttc aattccgctt cttccatgtc
     6361 taaaaccata tcgtggattt cggtatcgaa aaactcccaa aacgccttca gccccatatc
     6421 ttgcggccat ttatccttat cgatgtccca acctgccagc tccgcctcga aaatctggcg
     6481 gtagcgttcg tcgaagtagg aaacgacggc ttccggttcg tcgaactgcg gaacgaggaa
     6541 gacggaacag ttggcacgaa gctgctctat ggtcaggtcg ggcatatttt cgtcggtgct
     6601 tttgagccat tccaaaaagc gcgcggtcgg cttgaggacg acggcggtgc ggtcaacaaa
     6661 atacatggtt ttctttctca atcatcttgc ggtgtcggga tatgctgtct gaacgttcgg
     6721 ttttcagacg gcatagcatc aatgggtcat gacctgttgc aggaactgct tggcgcgttc
     6781 gtgtttcggg ttggtaaaaa acgcttccgg cgtttcgtct tcgaggattt gccctttatc
     6841 gacgaaaatc acgcggtcgg caacttcgcg ggcaaacccc atttcgtggg ttacgcacat
     6901 catcgtcatg ccgctttctg ccaagtcttt catcactttc aacacttcgc cgaccatttc
     6961 ggggtcgagt gcggaggtcg gttcgtcaaa caacattacg cgcggttcca tcgccaaacc
     7021 gcgtgcaatc gccacgcgtt gctgctggcc gccggaaagt tgggaaggga aggcgtcttt
     7081 tttgtgtgcc agtccgacgc gttccaaaag ctccattgcc tttttctccg cctgttccgc
     7141 attttgcccc ttaaccttca tcggtgcgag ggtaatgttt tccaatacgg tcaggtgcgg
     7201 gtagaggttg aagccttgga atacgaagcc gacttcttcg cggattttgt tcaaatcggt
     7261 tttggggtcg gcaacgttga caccgtccac ccaaatctcg ccgctttcga tgctttcaag
     7321 ctggttgacg gtgcggatga gtgtggattt gccgctgccc gaaggcccgc agacgacgac
     7381 cacttcgcct tttttgattt ccaagtttac gccgttgatg acgtgcaggt ctttgaaatg
     7441 tttgtgtacg tttttgaatt tgatcatgtc ttttctttca gacggcattt gccgttgcag
     7501 gttgtcgtta cgggagctcc atatgatgaa gcgtgtagcg tctgccgtca aaaaaacggt
     7561 cgttcggatt ggtcaggcag gctgcaagcg gcagtatcag gggtaaaagc aggtatttcg
     7621 tcatcggctt actccctttt cagacggcct tgcccgccag ataattgctc aacgccacat
     7681 atttctccgg cgcgatatgt tcggcgcggt cttgcggatt gatgccgact gcctgcaaat
     7741 catcgtcgcc tgcaagctct ttcagattgt tgcgtatggt tttgcggcgt tggtggaagg
     7801 cgagtttcac gagtttggcg aaatgctcga aatcgtccgc cttgccgata cggtgtttca
     7861 ccggaatcat gcggacgacg gccgaatcca ctttcggcgc ggggtcgaac gattcgggcg
     7921 gcacgtcaat cagcatttcc atatcgaaga aatattgcag catcacgccc aagcggccgt
     7981 agtcgttgct tttcggcgcg acaaccatac gatcgaccac ttctttttgc agcataaagt
     8041 gcatatcgac gacatcgtcc gccacctccg ccagcttgaa caaaagcggc gtggaaatgt
     8101 tgtagggcag gttgccgacg attttctttt tgcctgcgat gccgttgaaa tcaaactgca
     8161 atacatcgcc ttcgtgaatc accagtttat ccgcaaacgg cagcgttttc agacggcata
     8221 cgatgtcgcg gtcgatttcg acaacgtgca ggcggttcag ctttttcgcc aaaggttcgg
     8281 taatcgccgc caaacccgga ccgatttcaa tcacgacatc atccgcctgc gggcgcacgg
     8341 cgttgacaat atcgctgata atccgcgtgt cctgcaaaaa attctgcccg aaacgcttgc
     8401 gggctttgtg ttctttcatc gtgtttcctt ttcggttgaa accccgccct ttagggcggt
     8461 agaatcagac tctatttggg aggggcgtaa ctccttccaa atcaggacgg cacatagggc
     8521 ggtgctttat gtgtcgtcct gtgtgttgaa acataaatgt gtttacagta tccgtttgat
     8581 gtcggcattg taaccgaaaa cggcagggcg tgataatgct gtttgaaggc ttgccgtgtt
     8641 tggcggtttg gtgcaaaaac cggctgtctg ccgttttgcc tgttggagga ttgaacgtgt
     8701 ctgaaaatct gcttgaaatc gaaacccatc ccttcgattc cgtgttgccg ccgaaggctg
     8761 cggtcatgat gatggggacg tttccgccca aggaagacaa acgcgcgatg cagtttcatt
     8821 atccgaattt ccaaaacgat atgtggcgcg tttacgggct ggtgtttttt aatgatgcgg
     8881 cgcatttcca aaggttgtct gaaaaagcgt ttgatgccga gaaaatcaag gcgtttttgc
     8941 acgaacgggg gattgcgtcc tgtccgaccg tgttgaaggc ggcaagggaa cacggcaatg
     9001 cgtccgacaa gtttttaaag gtagtcgaaa ccgtcgattt ggcggcggtg ttggcgaaaa
     9061 taccagactg ccgccatatt tgtacgacag gcggcaaggc gacggaaatc ctgctcgata
     9121 ttcagggtgg cggcatcaaa atgccgaaaa cgggcgaaac cgtgccgttt ccgtttgccg
     9181 gacgggattt gacgctgacg cgcctgcctt cgacttcgcg tgcctatcct ttgagtttgg
     9241 cgaaaaaagc ggcggcgtat cgggcgtttt ttgaaatggc gggcttgtgt gaaaaacagt
     9301 tataattgcc gacaatttcc cgttcggacg gcatgtttga aaaatatagt ggattaacaa
     9361 aaatcaggac aaggcggcga agccgcagac agtacaaata gtacggcaag gcggggcaac
     9421 gccgtaccgg tttttgttaa tccactataa attgccgtct gaaaatttga agcacaagga
     9481 cgaaatctga tgaagaacta ccacgcgccc gacgagaagg gctttttcgg cgaacacggc
     9541 gggctgttcg tgtccgagac cctgattccg gctttgcaag agctggcgga tgcctataag
     9601 gcagcgaaaa acgatcctga attttgggaa gcgttccgcc atgatttgaa acattatgtc
     9661 ggcaggccca gccccgttta ccacgccgcg cggttgtccg aacatctggg cggcgcgcaa
     9721 atctggttga agcgcgaaga cttgaaccac accggcgcgc acaaagtcaa caacaccatc
     9781 ggtcaggcac tgttggcaaa acgcatgggt aaaaaacgcg tcatcgccga aaccggcgcg
     9841 ggtcagcacg gcgtggcgag tgccaccgtt gccgcacgct tcggtatgac ttgcgacgtg
     9901 tatatgggcg cggacgacat ccaacgccaa atgcccaacg tgttccgtat gaaattattg
     9961 ggtgcgaacg tggtcggtgt agaaagcggc agccgcacgc tgaaagacgc gatgaacgaa
    10021 gccatgcgcg aatgggtcgc ccgcgtggac gacacgttct acatcatcgg taccgccgcc
    10081 ggccccgcgc cgtatcccga aatggtgcgc gatttccaat gcgtgattgg caacgaagct
    10141 aaagcgcaga tgcaggaagc catcggcaga cagcccgacg ttgccgttgc ctgcgtgggc
    10201 ggcggatcga acgccatcgg tttgttccac ccgtatatcg gcgaagaaaa cgtgcgcctc
    10261 gtcggcgtgg aggctggcgg tttgggcgtg aacacccccg atcacgccgc gccgattact
    10321 tcgggcgcac cgattggcgt attgcacggt ttccgcagct atctgatgca ggacgaaaac
    10381 ggtcaggttt tgggtacgca ctctgtttcc gcaggcttgg attaccccgg catcggcccg
    10441 gaacacagcc atctgcacga catcaagcgc gtcgaataca ctgttgccaa agacgacgaa
    10501 gcactcgaag cctttgactt gctctgccgc ttcgagggca tcatccccgc gctcgaatcc
    10561 agccacgccg ttgcttgggc ggtgaaaaac gcgccgaaaa tgggcaaaga ccaagtgatt
    10621 ctggtcaacc tatccggtcg tggcgacaaa gacatcaata ccgtcgcgaa gctcaaaggg
    10681 attaaactgt aacctcgtcc gtct
//
LOCUS       DAWKQB010000060        10013 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.060, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000060 DAWKQB010000000
VERSION     DAWKQB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10013
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.060"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            535..1836
                     /gene="bioA"
                     /locus_tag="WJL14_001156"
     CDS             535..1836
                     /gene="bioA"
                     /locus_tag="WJL14_001156"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ7656229.1"
                     /translation="MPSEHQHISSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQVVETQIKQMAHVMFGGLTHESAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQARGLMAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSTLPQRYFVDNPKSRFDDEWDGADLQPVRALFEAHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGLLTELADKTVQILKEHSK"
     gene            1833..2480
                     /gene="bioD"
                     /locus_tag="WJL14_001157"
     CDS             1833..2480
                     /gene="bioD"
                     /locus_tag="WJL14_001157"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ7656230.1"
                     /translation="MKGVYFVSGIDTDIGKTIATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QQLAAQYEIVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILITSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHVAQDSLNYLKCRLKDSFPEAEWLELAKTDAV"
     gene            2495..2971
                     /locus_tag="WJL14_001158"
     CDS             2495..2971
                     /locus_tag="WJL14_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ7656231.1"
                     /translation="MEHLFEEWLPDLPADVSDGIGLPMSRLLKARSLTAALRALPHLF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVFGEGCGRYFAARRSRFSHHGEEMLLTEYFLPELKRFIG"
     gene            3001..3891
                     /gene="ubiA"
                     /locus_tag="WJL14_001159"
     CDS             3001..3891
                     /gene="ubiA"
                     /locus_tag="WJL14_001159"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ7656232.1"
                     /translation="MNPKSPLFLRLSDRLDVYIRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            4076..4525
                     /gene="ptsN"
                     /locus_tag="WJL14_001160"
     CDS             4076..4525
                     /gene="ptsN"
                     /locus_tag="WJL14_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ7656233.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            4527..5489
                     /locus_tag="WJL14_001161"
     CDS             4527..5489
                     /locus_tag="WJL14_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ7656234.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            5470..6324
                     /gene="rapZ"
                     /locus_tag="WJL14_001162"
     CDS             5470..6324
                     /gene="rapZ"
                     /locus_tag="WJL14_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ7656235.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEQEWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     ADDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            6405..7346
                     /gene="ylqF"
                     /locus_tag="WJL14_001163"
     CDS             6405..7346
                     /gene="ylqF"
                     /locus_tag="WJL14_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ7656236.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGSVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            7447..8049
                     /locus_tag="WJL14_001164"
     CDS             7447..8049
                     /locus_tag="WJL14_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ7656237.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVIQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLIEMPPTDEEEPTTVPSDTLPN"
     gene            8233..9906
                     /gene="recN"
                     /locus_tag="WJL14_001165"
     CDS             8233..9906
                     /gene="recN"
                     /locus_tag="WJL14_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ7656238.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPTLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
BASE COUNT         2448 a         2523 c         2718 g         2324 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat aaaatagggc tgtaaccttc aatcggaatt
       61 tgttgcctgc gggatatacg gtatgaatgt ttgggatata tggagcagga tggtggaaat
      121 ctatcataag tataagaagc cgtgcctggt tttggcggtg gattttgtga tgggtatggt
      181 attcatagag ccgaatgagg agccgtgcat cggtaggtgc tatgcgccta tgtcggagtc
      241 ccctgatttt gctaacgctg ttgcgatggc tgttgctatg atctgtatcg tatggattgc
      301 cgttttgtct atgactgatt gaagcgttgg cagaggctgt gtgaaacggt attgggcgtt
      361 gggccgtctg attccaatcg ggcttgagga atgcgaaacg gtgtgcgctt atactgcgga
      421 cgatttgttt cgcggttttg cgcccgaaac ggatggagag gtgtgggaaa cggtctgtcg
      481 gagtagaata cgcgttttgc gtttgaatac agtaagaaga aaagagagaa acttatgccg
      541 tctgaacatc aacacatatc atcattgctc gattttgacc gtacccatct gcttcatccc
      601 tatacttcca tgaccgatcc gctgcccgtt tatcctgtca aacgtgcaga aggggtgttt
      661 atcgaattgg cggacggcac gcggttgatt gacgggatgt cgtcttggtg gtgtgcgata
      721 cacggttaca atcatcctgt tttgaatcag gtggttgaga cgcagattaa gcaaatggcg
      781 cacgtgatgt tcggcggttt gacgcacgag tcggcggtgg aactgggcaa gttgttggtc
      841 ggaattctgc cgcaggggct ggaccgtatt ttttatgcgg attcgggttc ggtttcggtg
      901 gaagttgcgt tgaaaatggc ggtgcaatac cggcaggcgc ggggtctgat ggcgaagcag
      961 aatattgcga cggtgcggcg cgggtatcac ggcgatactt ggaacgcgat gtccgtctgc
     1021 gatccggaaa cggggatgca ccatattttc ggcagcactc tgccgcagcg ttattttgtc
     1081 gataatccga aaagccgttt cgatgatgaa tgggacgggg cggatttgca gcctgtccgc
     1141 gccttgtttg aagcgcatca tgcggatatt gccgccttta ttttggaacc ggtcgtgcag
     1201 ggcgcaggcg gcatgtattt ttatcatccg cagtatcttc gcggattgcg tgatttgtgc
     1261 gacgaatttg atatcgtgtt gatttttgac gaaatcgcta ccggattcgg gcgcacgggc
     1321 aagatgtttg cctgcgaaca cgcggaggtc gtgccggata ttatgtgtat cggcaagggc
     1381 ttgagcggcg gctatatgac gctggcggca gcaatcactt cgcaaaaagt taccgaaacg
     1441 atttcgcgcg gcgaagcggg cgtgtttatg cacggcccga cgtttatggc aaacccgctg
     1501 gcgtgtgccg ttgcctgcgc ttcggtcaaa ctgcttttgt ctcaagactg gcaggcaaat
     1561 atccgccgca ttgaaagcat cttaaaaggc cgtctgaaag ccgcgtggga tattcgcggc
     1621 gtgaaagacg tacgcgtttt gggtgcaatc ggcgtgatcg agctggataa aggcgtggat
     1681 atggcgcgtt ttcaagcgga ctgcgtggcg cagggcattt gggtgcgccc attcggcagg
     1741 ctggtgtacc tcatgccgcc ttacatcatt tcagacggcc tcttgaccga acttgccgac
     1801 aaaaccgtac aaatcttgaa ggaacacagc aaatgaaagg cgtttacttc gtcagcggca
     1861 tagacacgga catcggcaaa accatcgcca ccggcgtatt ggcaaaacaa ttgttgcaac
     1921 aaggcaaaag cgtaatcacg caaaagcccg tgcaaaccgg ttgccaaaac atcgccgacg
     1981 acatcgccgt tcaccgcaaa attatgggca tcccgatgca ggaagcggac aaacaagggc
     2041 tgaccatgcc tgaaatcttc agttatccag cttcgcctca tctcgctgcc cgtcttgacg
     2101 gcagggcttt ggacttggac aaaatccgca ccgccacgca gcaactggcg gcgcaatacg
     2161 aaatcgtctt ggtcgaaggc gcgggcggtt tgatggttcc gctgacggaa aacctgttaa
     2221 ccattgatta tatccgtcag caaggctatc ccgtcatcct cataacgagc ggacggctcg
     2281 gcagcatcaa ccacaccctg ctcagtttcg ccgcgctcaa acaatatggc atccgcctgc
     2341 acagtctcgt gttcaatcac atccacgaca gccgcgacgc acacgtcgct caagacagct
     2401 tgaactatct gaaatgccgt ctgaaagaca gtttccccga ggcggaatgg ttggagttgg
     2461 caaaaacaga tgcggtataa agattgggaa aaatatggaa cacctatttg aggaatggtt
     2521 gcccgacctg cccgccgacg tttcagacgg catcggcctg ccgatgagcc ggttattgaa
     2581 agcccggtcg ctgactgccg cattgcgcgc cttgccgcat ctgttttcgg tagaactgct
     2641 gaaactgggc gaattggaaa cggaatgcgg agggcggctg gtgcgcgaag ttttgttgaa
     2701 gctggacggt atccctgttg tcgcggcaag gagcgaatgc cgtatcggtt cggcgttttg
     2761 gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgaa cgtctgtttc aagccgattt
     2821 ggaaggggcg cgttcggcgt ttgagtttgc cgttttcggc gaaggatgcg gacggtactt
     2881 tgccgctcgg cgttcgcggt tttcccatca cggcgaggaa atgctgctga ccgagtattt
     2941 tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct gcgcggcaat
     3001 atgaatccta aatccccttt atttttacgc ctgtccgatc gtttggacgt ttatatcaga
     3061 ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac ctactgggcg
     3121 ttgtggctgg cttcagacgg tattcccgat ttggcggtat tggcggcgtt tacaatcggc
     3181 acgtttttaa tgcgcagtgc cggctgcgtc atcaacgatt ttgccgaccg cgattttgac
     3241 ggggctgtcg agcgcactaa aaaccgtccg ttcgcacagg gcagggtcaa gaaaaaagaa
     3301 gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat tccgctgaat
     3361 catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta cccgtttacc
     3421 aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt cggtatcccg
     3481 atggcgtttg ccgcagtggg caacagcgtc cccgttgaag catggatact ttttaccgcc
     3541 aatgtgttat ggactctggc gtatgacacg gtttatgcca tggcggacaa agaagacgat
     3601 ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat cgccgccgtt
     3661 atgctgtgtc atggcggctt taccctgctg atggcagtat tgggggcggt tatcggtgcg
     3721 gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg ccaatatgcc
     3781 gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa caacagaatt
     3841 ggttgggtgt ggtttaccgc catttttgcc catacgtttt ttgcgaaata aggcagggca
     3901 atgccgtctg aagagccgca aactgctttg gacggcattt ctatctgtgc cgaaaagcgt
     3961 taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg gattgagatt
     4021 tgccctggca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga agcttatgag
     4081 ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg tgggcagtaa
     4141 aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat tgtcccatgc
     4201 tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt tggggcaggg
     4261 cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg cgttcatccg
     4321 cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaaa ccggtttccc tggtttttat
     4381 cttgctggtt ccggaaaatg cgacgggcga gcatttggaa gtattgtcca aactggccgg
     4441 caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg cggaagaagc
     4501 ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga ctgtttgacg
     4561 acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg gacaaccgta
     4621 tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat ttcatccacc
     4681 ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt ctcgaatcgg
     4741 gggaaacggg ttatcagttc ggcgacctgt tcgatatttc catgtctttg gttattgtgg
     4801 caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac gatattccac
     4861 tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt tacctgcaac
     4921 gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt gaaatcggcg
     4981 tgctgattac cggacattct ggcttgggca agagcgaatt ggcgttggaa ctgatttcgc
     5041 gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt ccggaaacat
     5101 tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc ttggggatac
     5161 tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc ctgcaactca
     5221 ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg ttgagtatcc
     5281 gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggtaacgctg cctgtcgcgg
     5341 tcggacgcaa ccttgccgta ttggttgaag cggcggtgcg caattatatt cttcagttgc
     5401 gcggtaagga tagtacgcgc gaatttttgg aacgccatca gacgcagctt aaagaaaacg
     5461 aacagaatca tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa atccgtcgca
     5521 ctgcgccaaa tggaagattc gggttatttc tgcgtggaca acctgccctt ggaaatgttg
     5581 cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc ggtcagcgtc
     5641 gatgtacgtt ccggcattga catcggacag gcgcgggaac agattgccta tctgcgcgga
     5701 ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt ggtccgccgg
     5761 ttttccgaaa ccaggcgagg acatcctctg agtaatcagg atatgacctt gttggaaagc
     5821 ttaaagaaag aacaggaatg gctgttcccg cttaaagaaa tcgcctattg tatcgacact
     5881 tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa ggtcgaacgt
     5941 accgggctgc tggtgatttt ggagtccttc gggttcaaat acggcgtgcc gaacaacgcc
     6001 gattttatgt tcgatatgcg cagcctgccc aacccgtatt acgatcccga gttgaggcct
     6061 tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct tgtgcaggaa
     6121 atggcggacg acatcgaaaa atttctgacg cattggctgc cgcgtttgga ggatgaaagc
     6181 aggagctacg ttaccgtcgc catcggctgt accggagggc agcatcgttc ggtctatatt
     6241 gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg gcacagacag
     6301 gcgcaaaacc tgtcagaccg ctaattccgt caaaccatta tgccgtctga aaccccgtcc
     6361 tccgtttcag acggcatttc aaatattgaa aagaaagaac agacatggca atccaatggt
     6421 ttcccggcca tatgaacaag gcgaaaaaag ccatcgccga gcgtgcaaaa agcgttgata
     6481 tggtgattga aatgctggac gcgcgtatgc ccgcctccag cgaaaacccc ctgcttgccc
     6541 agctttccaa aggtaaaccc aaacttaaaa tcctcaacaa acaagatctt gccgaccccg
     6601 agcgcaccaa aatctggctc gaacactata acagccgccc cgacacctgc gccatcgccc
     6661 tcgattcctc cgaaacaggc gcacacggca aaattaccca agcctgccgc gcgatgattc
     6721 cccaccggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt cccaacgtcg
     6781 gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc ggcaacgaac
     6841 ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg ctgtacgaca
     6901 cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac ctcgccgccg
     6961 gcggttcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa cttttagact
     7021 acctccgccg ccactacctc cctatgttgc aagaacgcta ccaagccgac aaagacccca
     7081 gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc ggcgcagtcc
     7141 tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catccttacc gacttccgtg
     7201 aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact tggctcaaaa
     7261 aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa gcagagcgta
     7321 aagggcagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt tcaggcagct
     7381 tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt cagcacggat
     7441 acccgtatga ccgacaaaat ttctcccgac gcactaattg aagccgcact gctgacccaa
     7501 accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact gtcgcaggac
     7561 aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc gttgcaactg
     7621 gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga gcggctgggc
     7681 agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac actggcgatt
     7741 atcgcctacc agcagcccgt aacgcgcggc gacatcgaag gcatacgcgg tgtggcggtg
     7801 tcgcagaacg tgatccagac cttgcaggat cgggggtgga ttgaagtcat cggacatcgg
     7861 gacacattgg gaaaacccgc attgtgggca acaacggcaa cgtttctcag cgatttgcgc
     7921 ttggacggtc tggaagaact tcctccgctg accgaactgg gcgaactggt tttgcccgat
     7981 ttgatagaaa tgccgcctac ggatgaagaa gagccgacaa ccgtaccatc cgataccctg
     8041 cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc attgcaacaa
     8101 ataagcagat aaaaacaagc actaagaaaa attaaggaaa aacttatttt taatttaaaa
     8161 aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta gaattgctaa
     8221 ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt tgaaaatctg
     8281 aatctggatt ttcaaagcgg ctttaccgta ttgactggag aaactggcgc gggcaagtcc
     8341 attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag ccaagtccgc
     8401 agcggcgcaa aagaagcgca gttgtcggca ttgttcgata tttcccattt gcctacttta
     8461 aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact cagtatccgc
     8521 cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc cgctaccttg
     8581 gcgcaactca aagccgtcgg cagccagctt atcgacatcc acgggcaaaa cgcccatcat
     8641 tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg cagcagggcg
     8701 caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa agcattgcag
     8761 gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga atggcagttt
     8821 aacgaattga atcagttgga cattaaacaa ggcgaatggg aagccctcag ccaaagccac
     8881 gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg aagcaagatt
     8941 gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt ggccaatctg
     9001 caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat cgaggccgaa
     9061 ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggac gcagcgacat caaccccaat
     9121 gaattggcgg cacaggaaca gcgtatgggc gaactgatgg ggatggcgcg gaaataccgg
     9181 atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct gcaaagcctg
     9241 caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt tgccgaatat
     9301 caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg tttgagcggc
     9361 gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga catcgtcctg
     9421 ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt tgccgccaac
     9481 aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt ggcgcgtatc
     9541 agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct gatttttgat
     9601 gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc attacgtgcg
     9661 ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc atcctgcggc
     9721 gaaaaccact ggcgggtgcg caagcacagc gagggagagc aaaccgtcag cgaaatcagt
     9781 atattggatg aaatccaacg gatcgaagag gttgcccgta tgttgggcgg agaagtcatt
     9841 accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa aaatagttta
     9901 ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa tagacagaac
     9961 tcagataaat ccgtattatc acgctttctt aatcacttga acaagtgatt gtg
//
LOCUS       DAWKQB010000061         9997 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.061, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000061 DAWKQB010000000
VERSION     DAWKQB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.061"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(119..1336)
                     /locus_tag="WJL14_001166"
     CDS             complement(119..1336)
                     /locus_tag="WJL14_001166"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ7656239.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLTAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJL14_001167"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="WJL14_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ7656240.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="WJL14_001168"
     CDS             complement(2348..2812)
                     /locus_tag="WJL14_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ7656241.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="WJL14_001169"
     CDS             3029..3805
                     /locus_tag="WJL14_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ7656242.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4050..4904
                     /locus_tag="WJL14_001170"
     CDS             4050..4904
                     /locus_tag="WJL14_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ7656243.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLSSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLSILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            4945..6153
                     /gene="pncB"
                     /locus_tag="WJL14_001171"
     CDS             4945..6153
                     /gene="pncB"
                     /locus_tag="WJL14_001171"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ7656244.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELDYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYIWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6245..7963
                     /gene="argS"
                     /locus_tag="WJL14_001172"
     CDS             6245..7963
                     /gene="argS"
                     /locus_tag="WJL14_001172"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ7656245.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLAFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRIGRLKADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEATKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8481..9899
                     /locus_tag="WJL14_001173"
     CDS             8481..9899
                     /locus_tag="WJL14_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7656246.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2293 a         2942 c         2521 g         2241 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cggagacttt gcacacggtg tcgtcgccat caatagaagc cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 cgaccgcgcc caaaatctga taggcgacac cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtactttccg gcgaattcta ctgaacggat ttgcaagact ttcgagccga
      721 ggcttgccaa ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgcgg atcggtggtg tacacgccgt ccacgtcggt atagatttgg cattcgtccg
      841 ctttgagggc tgcggcaagc gcaacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgccggcgg
      961 taaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttgt
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccgcctgccg tttgacagcg gtcagcgaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcagac agcgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg tcccctgtat ttcgatgatt tttcccgatg
     1681 cggtcatcac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgta cgcgtaccgc cgtctgcctg aatcacgtcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ccacgaccgc gcgcagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tgcccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccaa atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgct
     2881 gtttcattat agacccgccg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gagacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag aatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacgc ggcaatcaag ctgtcggttc agaaaaacca acatcggcac gcctaaaacc
     3901 gtattcacaa tctactcctt ttcaaaacat ttgcatttaa aagccgttat aatgccgtct
     3961 gaacatctgc ccgaccacat tatgcgtgaa tgccggcaga ttgttttctt ttgtaaactt
     4021 atattaaaat ccacttacca attcacgcca tgccgcccat ccctgcccca tctgcaccat
     4081 ccgagcacac tgtcgcatgg gtattcggtc aacccgttac cgatttgccg caggatttgt
     4141 ttatcccgcc cgacgcactg aaagtcgtat tgagcagttt tcaaggccct ttggatctgc
     4201 tgctctacct tatccgcaag cagaacatcg atgttctcga tattccgatg gtgaagatta
     4261 ccgaacagta tctgcactac atcgcccaaa tagaaaccta tcagtttgat ttggcggcgg
     4321 aatatctttt gatggcagca atgctgattg aaatcaaatc gcgcctgctg ctgccgcgta
     4381 ccgaaaccgt cgaagacgaa gaagccgacc cgcgtgccga gttggtgcgc cgcctgctgg
     4441 cttacgagca gatgaagctg gcggcacaag ggttggatgc gcttcctcgt gcgggccggg
     4501 atttcgcatg ggcatacctg ccactggaaa ttgccgtcga agccaagctg cccgaagtct
     4561 atattaccga cttgacgcag gcgtggctga gtattttgtc tcgggcaaaa catacgcgca
     4621 gccacgaagt tatcaaagaa accatctccg tgcgcgcgca aatgacggca atcctgcgcc
     4681 gtttgaacaa acacgggata tgcaggtttc acgacctgtt caatcccgaa cagggcgcgg
     4741 cttacgtggt cgtcaacttc atcgcactgt tggagcttgc caaagaaggt ttggtcggaa
     4801 tcgtacagga agtcggtttc ggagaaatcc gaatcagcct caatcatgag ggggcgcatt
     4861 cagacggcat ttccggcaca cggggcgggc gcgatgtgtt ctaatacgcc ccaagccgcc
     4921 accaaaaatc gggagacacg ccatatgacc ggcatcatac attcgttgct tgacaccgac
     4981 ctctacaaat tcactatgct gcaagtagtc ctgcaccagt ttccgcagac gcacagcctt
     5041 tacgaattcc gatgccgcaa cgcctcgacc gtctatccgc ttgccgacat caaagaagac
     5101 ttggaagtcg aactcgacgc gctctgccaa ctacgcttca cccacgacga actcgactat
     5161 ctgcgcagcc tgcgtttcat caaaagcgac tttgtcgatt atctcgaact cttccagctc
     5221 caacgccgct ttgtcgaagt cggcacagac gataaaggcc gtctgaacat ccgcatcgaa
     5281 ggtccgatga tacaggcgat gttttttgaa atcttcatcc tcgccattgt caacgaactt
     5341 tacttccgcc ggctggaaac ccctgccgtt atcgaagaag gtgaacgccg gcttcaagcc
     5401 aaagccgcgc gcctgaaaga aatcgccgcc gcacaaaacc ccgacgaacc gcccttcctg
     5461 atttccgact tcggcacgcg ccgccgctac aagctcgcgt ggcaggaaca cgtcatccgc
     5521 accctgctcg aagccgcccc aagcatcgtg cgcggcacca gcaatgtctt tctcgccaaa
     5581 aaactcggca tcacgcccat cggcaccatg gcgcacgaat tcctccaagc cttccaagcc
     5641 ctcgacgtgc gcctgcgtaa tttccaaaag gccgcgctcg aaagttgggt acacgaatac
     5701 cggggcgatt tgggcgttgc cctgaccgac gtggtcggta tggatgcctt cctgcgcgat
     5761 ttcgacctct atttcgccaa actctttgac gggctgcgcc acgacagcgg cgacccttac
     5821 atttggggcg acaaagccta cgcccactat caaaagctca aaatcgacag ccgcaccaaa
     5881 atgctgacct tctccgacgg gctggacatc gaacgctctt gggcattaca ccaatatttc
     5941 aaagaccgct tcaaaaccgg cttcggcatc ggcaccaacc tcaccaacga tatggggcat
     6001 acacccttaa atattgtgtt gaaactggtc gaatgcaacg ggcagtccgt cgccaagctg
     6061 tccgactctc cgggcaaaac catgaccaac aacagcacct tcctcgccta cctgcgccaa
     6121 gtgttcgacg tacccgaacc cgaaacgccg taaaccggca gaaaaagcgc acaattcctg
     6181 tttctgccgc ataaaatctt ttaaaatacc gcctgatttg aatttaaccg aaagaccgaa
     6241 cttcatgaac ctacatcaaa ccgtcgaacg cgaagccgcc gccgcctttg ccgccgcagg
     6301 catcgccgac agtcccgttg ttttgcagcc gaccaaaaat gccgaacacg gcgatttcca
     6361 aatcaacggc gtgatgggtg cggcgaaaaa agccaaacaa aacccgcgcg agttggcgca
     6421 aaaggtcgcc gaagcattgg cggacaacgc cgtgattgaa agcgcggaag tcgccggtcc
     6481 gggcttcatc aacctgcgcc tgcgccccga atttctcgcg caaaacattc agacggcctt
     6541 gaacgacgct cgtttcggcg tggcaaaaac cgacaaaccg caaaccgtcg ttatcgacta
     6601 ttcttcgccc aatctggcga aggaaatgca cgtcggccac ctgcgttcca gcatcatcgg
     6661 cgacagcatt tcgcgcgtgt tggcatttat gggcaatacc gttatccgtc aaaaccacgt
     6721 cggcgactgg ggtacgcagt tcggtatgtt ggtcgcttat ttggtcgagc agcaaaaaga
     6781 caatgccgcg ttcgagctgg cggatttgga gcagttttac cgcgccgcca aagtgcgctt
     6841 tgacgaagac cctgcctttg ccgacaccgc acgcgaatac gttgtgaagc tgcaaggcgg
     6901 cgatgaaacc gttttggcgt tgtggaaaca gtttgtcgat atttcgctct cgcacgccca
     6961 agccgtttac gacacgctgg gcttgaagct gcgtcctgaa gacgtggcag gcgaatcgaa
     7021 atacaacgac gatttgcagc ccgtggtcga tgatttggtt caaaaaggtc tggcggttga
     7081 ggacgacggc gcgaaagtcg tgttcttgga cgaatttaaa aacaaagaag gcgaacccgc
     7141 cgcatttatc gtgcaaaaac aaggcggcgg cttcctctac gcctccaccg atttggcgtg
     7201 cctgcgctac cgcataggcc gtctgaaagc cgaccgcctg ctgtacgtcg tcgaccaccg
     7261 ccaagccctg cactttgagc aactctttac cacttcacgc aaagccggct atctgccgga
     7321 aaacgtcggc gcggcatttg tcggcttcgg cacgatgatg ggcaaagacg gcaagccgtt
     7381 taaaacgcgc agcggcgaca ccgtgaaact ggttgatttg ctgaccgaag ccgtcgagcg
     7441 cgccaccgct ttggtgaaag agaaaaatcc cgaattgggc gcggacgaag ccaccaaaat
     7501 cggtaaaacc gtcggcatcg gctcaatcaa atacgccgat ttgagcaaaa accgcaccag
     7561 cgattatgtg ttcgactggg acgccatgct ctcgtttgaa ggcaacaccg ccccctatct
     7621 gcaatacgcc tacacccgcg tgcaaagcgt gttccgcaaa gcaggcgaat gggatgcaaa
     7681 tgcgccaacc gttttgaccg aaccgctgga aaaacagctt gccgccgagc tgctgaaatt
     7741 tgaagacgta ctgcaaagcg tggcggacac ggcgtatccg cactacctcg ccgcctacct
     7801 ctatcaaatt gcgaccctgt tcagccgctt ctacgaagcc tgtccgatac tcaaagccga
     7861 aggcgcaagc cgcaacagcc gcctgcaact ggcaaaactc accggcgaca cgctgaaaca
     7921 aggcttggat ttgctgggca tcgatgtgtt ggacgtaatg taaaaccgca ccgcccgatt
     7981 gcggacaaca gcctcgccat ccttatccga atctgaaaaa agcggcgcga tacaccgtat
     8041 ccgccgcccc tcccaaaatg cgaaacagac aaacgccaag caagcaagca aaaattataa
     8101 ccccctttct gccgacgcac gcactttccg cgcggcgcat tccccttttt ccgcccctca
     8161 aacccgcctt ttctccaggc agggtttcag cccgcctcct ttccctgttt tccttccctc
     8221 aacacgcgtg cgcaccccct gccgcaccgt gccgcacttt cgcgctcgga cggcatcggt
     8281 ctgtcatccg gttctctgtt ttaaataccc ctgtttcaga aagaaattgc agatgtttca
     8341 acacacagga cgacacataa agcaccgccc tatatgttgc cctgatttgg aaggggttac
     8401 gcctcccaaa taaagcctga tcctgccgcc ccgaaggaca gatgtccgag tggcggggtt
     8461 tcaaccgaaa aggaaatacg atgaatattc acgccctgct ctccaaacaa tggacgctgc
     8521 cgccattcct gccgaaacgg ctgctgctgt ccctgctgat actgcttgcc cccaatgcgg
     8581 tgttttgggt tttggcactg ctgaccgcca ccgcccgccc gattgtcaat ttggactacc
     8641 ttcccgccgc gctgctgatc gccctgcctt ggcgtttcgt caaaattgcc ggcgtattgg
     8701 cgttttggct ggcggttttg tttgacgggc tgatgatggt gatccagctt ttccctttta
     8761 tggacctcat cggcgccatc aacctcgtcc ccttcatcct gaccgccccc accccttatc
     8821 agataatgac cgggctgttg ctgctgtata tgctggcgat gccgtttgtg ttgcagaaag
     8881 ccgccgccaa aaccgacttc cggcacattg ccgtctgcgc cgccgttgtg gcggcagccg
     8941 gctatttcac cggccatttg agttactacg accgggggcg gatggccaat atcttcggcg
     9001 caaacaactt ctattacgcc aaaagtcagg cgatgctcta caccgttagc cagaatgccg
     9061 actttattac cgccggcctg gtcgatcccg tcttcctccc cttgggcaat cagcagcgtg
     9121 ccgccacgca tctgaacgag ccgaaatctc aaaaaatcct ctttatcgtc gccgaatctt
     9181 gggggctgcc ggccaatccc gaacttcaaa acgccacttt tgccaaactg ctggcgcaaa
     9241 aagaccgttt ttcggtttgg gaaagcggca gttttccctt catcggcgcg acggtcgaag
     9301 gcgaaatgcg cgaattgtgc gcctacggcg gtttgcgcgg gttcgcactg cgccgcgcgc
     9361 ccgacgaaaa atttgcccgc tgcctcccca accgtttgaa acaagaaggt tatgccacct
     9421 ttgcgatgca cggcgcgggc agttcgcttt acgaccgctt cagctggtat ccgagggcgg
     9481 gctttcaaga aatcaaaacc gccgaaaacc tgatcggtaa aaaaacctgt gccattttcg
     9541 gcggcgtgtg cgacagcgag ctgttcggcg aagtgtcggc atttttcaaa aaacacgaca
     9601 agggactgtt ttactggatg acgctgacca gccacgccga ctatcccgaa tccgacattt
     9661 tcaaccacag gctcaaatgc accgaatacg gcctacccgc cgaaaccgac ctctgccgca
     9721 acttcagcct gcacacccaa ttcttcgacc aactggcgga tttaatccaa cgccccgaaa
     9781 tgaaaggcgt ggaagtcatc atcgtcggcg accatccgcc gcccgtcggc aacctcaatg
     9841 aaaccttccg ctacctcaaa caggggcacg tcgcctggct aaacttcaaa atcaaataac
     9901 aacaatgccg tctgaacgca ccaacagcct tcagacggca ttttgcagac agaccgaccc
     9961 ttcaagccca cttttttcat catctccgat aaattgc
//
LOCUS       DAWKQB010000062         9889 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.062, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000062 DAWKQB010000000
VERSION     DAWKQB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.062"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            115..834
                     /locus_tag="WJL14_001174"
     CDS             115..834
                     /locus_tag="WJL14_001174"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ7656247.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVITGEDEGTLVHC"
     gene            1017..1979
                     /gene="mafA"
                     /locus_tag="WJL14_001175"
     CDS             1017..1979
                     /gene="mafA"
                     /locus_tag="WJL14_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ7656248.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIQPYGNHMGNSAPSVEADNSHEGYGYSDEAVRRHRQGQP"
     gene            2019..3467
                     /gene="mafB"
                     /locus_tag="WJL14_001176"
     CDS             2019..3467
                     /gene="mafB"
                     /locus_tag="WJL14_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ7656249.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGNARGSVKNRVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHE
                     VHSPFDNHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKSNIVPRAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WANRMDDIRGIVQGAVNPFLMGFQGVGIGAITDSAVSPVTDTAAQQTLQGINHLGNLS
                     PEAQLAAATALQDSAFAVKDGINSARQWADAHPNITATAQTALAVAEAATTVWGGKKV
                     ELNPTKWDWVKNTGYKTPAVRTMHTLDGEMAGGNRPPKSITSNSKADASTQPSLQAQL
                     IGEQISSGHAYNKHVIRQQEFTDLNINSPADFARHIENIVSHPTNMKELPRGRTAYWD
                     DKTGTIVIRDKNSDDGGTAFRPTSGKKYYDDL"
     gene            3477..3683
                     /locus_tag="WJL14_001177"
     CDS             3477..3683
                     /locus_tag="WJL14_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656250.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            3815..4066
                     /locus_tag="WJL14_001178"
     CDS             3815..4066
                     /locus_tag="WJL14_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656251.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            4063..4434
                     /locus_tag="WJL14_001179"
     CDS             4063..4434
                     /locus_tag="WJL14_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7656252.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            4540..5289
                     /gene="mafB"
                     /locus_tag="WJL14_001180"
                     /pseudo
     CDS             4540..5289
                     /gene="mafB"
                     /locus_tag="WJL14_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            5255..5461
                     /locus_tag="WJL14_001181"
     CDS             5255..5461
                     /locus_tag="WJL14_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656253.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            5482..5751
                     /locus_tag="WJL14_001182"
     CDS             5482..5751
                     /locus_tag="WJL14_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656254.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNILSIHKEIQDETVNRVYTD"
     gene            5774..6076
                     /locus_tag="WJL14_001183"
     CDS             5774..6076
                     /locus_tag="WJL14_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656255.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            6160..6771
                     /locus_tag="WJL14_001184"
                     /pseudo
     CDS             6160..6771
                     /locus_tag="WJL14_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253822.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            6768..7085
                     /locus_tag="WJL14_001185"
     CDS             6768..7085
                     /locus_tag="WJL14_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656256.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            7155..7884
                     /locus_tag="WJL14_001186"
                     /pseudo
     CDS             7155..7884
                     /locus_tag="WJL14_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            7888..8289
                     /locus_tag="WJL14_001187"
     CDS             7888..8289
                     /locus_tag="WJL14_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656257.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            8448..8791
                     /locus_tag="WJL14_001188"
                     /pseudo
     CDS             8448..8791
                     /locus_tag="WJL14_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8934..9290
                     /locus_tag="WJL14_001189"
     CDS             8934..9290
                     /locus_tag="WJL14_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ7656258.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITENQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTESLVKNIIH
                     ALEYAIKNEKTVIFHF"
     gene            9463..9837
                     /locus_tag="WJL14_001190"
     CDS             9463..9837
                     /locus_tag="WJL14_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656259.1"
                     /translation="MIIQNEFNLYPSNMLPERFCYPEKYVRISNDTSLIPYIQPHNFH
                     WWFENYGTEGAEVAYIFRNSILPDLNLIPFASNGEWEAYFDGNDVTGNPRVIVINLDN
                     IENHEFFNSFEEWLELAIKDTW"
BASE COUNT         3326 a         1841 c         2052 g         2670 t
ORIGIN      
        1 atttaaattt aatccactat aacagaacga cacgccatat cattgtaatt ccgaccgttg
       61 gaaattcaga cggcccaact ccgaccgacg acattcaaga aagcaaggta tcccatgaca
      121 cagcaaatca aatacaaacg cgtattactg aaactctccg gcgaatccct gatgggttcc
      181 gatccgttcg gcatcaatca cgacaccatc gtccaaaccg tcggcgaaat tgccgaagtc
      241 gttaaaatgg gcgtacaggt cggcatcgtc gtgggcggcg gcaatatttt ccggggcgta
      301 tccgcccaag caggcagcat ggaccgcgcc actgccgatt acatgggtat gatggcgacc
      361 gtgatgaacg ccttggcact caaagacgca tttgaaactt taggcatcaa agcgcgcgta
      421 caatccgcac tgtctatgca gcaaatcgct gaaacttacg cccgtcctaa agccatccaa
      481 tatttggaag aaggcaaagt cgtgattttt gccgccggta ccggtaaccc gttcttcacg
      541 accgacactg ccgccgcatt gcgcggtgcg gaaatgaact gcgacgtgat gctcaaagcc
      601 accaacgtcg acggtgtata caccgcagac ccgaaaaaag acccgtccgc cacacgctac
      661 gaaaccatta cttttgacga agccttgttg aaaaacctca aagtcatgga cgcgaccgct
      721 ttcgccctct gccgcgaacg caagctcaat atcgtcgtct tcggcatcgc caaagaaggc
      781 tcgctcaaac gcgtcattac cggcgaagac gagggaacgc tggttcactg ctgattgacc
      841 atagtgtcgg cagatatagt cgcatatggg cttcagacag ccatttatta tatggagatt
      901 atagtggatt aaatttaacc agtacggcgt tgcctcgcct tgccgtactg gtttaaattt
      961 aatccactat atttacaatt ttgatacaat ttgtttttca tcaaaggaga aaatctatgc
     1021 aagcacggct gctgatacct attctttttt cagtttttat tttatccgcc tgcgggacac
     1081 tgacaggtat tccatcgcat ggcggaggta aacgctttgc ggtcgaacaa gaacttgtgg
     1141 ccgcttctgc cagagctgcc gttaaagaca tggatttaca ggcattacac ggacgaaaag
     1201 ttgcattgta cattgcaact atgggcgacc aaggttcagg cagtttgaca gggggtcgct
     1261 actccattga tgcactgatt cgtggcgaat acataaacag ccctgccgtc cgtaccgatt
     1321 acacctatcc acgttacgaa accaccgctg aaacaacatc aggcggtttg acaggtttaa
     1381 ccacttcttt atctacactt aatgcccctg cactctcgcg cacccaatca gacggtagcg
     1441 gaagtaaaag cagtctgggc ttaaatattg gcgggatggg ggattatcga aatgaaacct
     1501 tgacgactaa cccgcgcgac actgcctttc tttcccactt ggtacagacc gtatttttcc
     1561 tgcgcggcat agacgttgtt tctcctgcca atgccgatac ggatgtgttt attaacatcg
     1621 acgtattcgg aacgatacgc aacagaaccg aaatgcacct atacaatgcc gaaacactga
     1681 aagcccaaac aaaactggaa tatttcgcag tagacagaac caataaaaaa ttgctcatca
     1741 aaccaaaaac caatgcgttt gaagctgcct ataaagaaaa ttacgcattg tggatgggac
     1801 cgtataaagt aagcaaagga attaaaccga cagaaggatt aatggtcgat ttctccgata
     1861 tccaaccata cggcaatcat atgggtaact ctgccccatc cgtagaggct gataacagtc
     1921 atgaggggta tggatacagc gatgaagcag tgcgacgaca tagacaaggg caaccttgat
     1981 tcacactacc ataaccgctt gctaccaagg aaaacaaaat gaatttgcct attcaaaaat
     2041 tcatgatgct gtttgcagca gcaatatcgt tgctgcaaat ccccattagt catgcgaacg
     2101 gtttggatgc ccgtttgcgc gatgatatgc aggcaaaaca ctacgaaccg ggtggtaaat
     2161 accatctgtt tggtaatgct cgcggcagtg ttaaaaatcg ggtttacgcc gtccaaacat
     2221 ttgatgcaac tgcggtcggc cccatactgc ctattacaca cgaacggaca ggatttgaag
     2281 gcattatcgg ttatgaaacc catttttcag gacatggaca tgaagtacac agtccgttcg
     2341 ataatcatga ttcaaaaagc acttctgatt tcagcggcgg cgtagacggt ggttttaccg
     2401 tttaccaact tcatcggaca gggtcggaaa tccatccgga ggatggatat gacgggccgc
     2461 aaggcagcga ttatccgccc cccggaggag caagggatat atacagctac tatgtcaaag
     2521 gaacttcaac aaaaacaaag agtaatattg ttccccgagc cccattttca gaccgctggc
     2581 taaaagaaaa tgccggtgcc gcctctggtt ttttcagccg tgctgatgaa gcaggaaaac
     2641 tgatatggga aagcgacccc aataaaaatt ggtgggctaa ccgtatggat gatattcgcg
     2701 gcatcgtcca aggtgcggtt aatccttttt taatgggttt tcaaggagta gggattgggg
     2761 caattacaga cagtgcagta agcccggtca cagatacagc cgcgcagcag actctacaag
     2821 gtattaatca tttaggaaat ttaagtcccg aagcacaact tgcggctgca accgcattac
     2881 aagacagtgc ttttgcggta aaagacggta tcaattccgc cagacaatgg gctgatgccc
     2941 atccgaatat aactgcaaca gcccaaactg cccttgccgt agcagaggcc gcaactacgg
     3001 tttggggcgg taaaaaagta gaacttaacc cgaccaaatg ggattgggtt aaaaataccg
     3061 gctataaaac acctgctgtt cgcaccatgc atactttgga tggggaaatg gccggtggga
     3121 atagaccgcc taaatctata acgtccaaca gcaaagcaga tgcttccaca caaccgtctt
     3181 tacaagcgca actaattgga gaacaaatta gtagtgggca tgcttataac aagcatgtca
     3241 taagacaaca agaatttacg gatttaaata tcaattcacc agcagatttt gctcggcata
     3301 ttgaaaatat tgttagccat ccaacaaata tgaaagagtt acctcgcggt agaactgcgt
     3361 attgggatga taaaacaggg acaatagtta tccgagataa aaattctgac gatggaggta
     3421 cagcatttag accaacatca ggtaaaaaat attatgatga tttataggaa aaagccatga
     3481 atatactatc cataaataat caaaactcaa ctatttcact aactcaagat gaagtttttg
     3541 ttttacgagc tatcttgaat gagatatatg cgggcgtatg tgtagattca agagaatttg
     3601 aaaatgtatc tggtgttaga aaacatgaag tagataattt acaacaacag tttgctggaa
     3661 tttataaaaa aatgacaact taacaaccca aatttttgtc agagcctagt gcaaattaca
     3721 actatgattc tattgtagcc gaaatgaaag aaaaaaatca tgggttggcg acagggttga
     3781 tgttgttaat atgcctgatg gagcacctac tagtatggat aacacgcgta ttatggcagc
     3841 acgtgaagca ggagtaaaag tggaagcgaa tgttcataat tttaatgacc gattatcatc
     3901 aaaagagaga atcaggttta agcatgatgg tattgagcct caaacttggg gagaagctat
     3961 ccagctacga attagaaagc aagaaacaca aaaaggagtt ccagaagggt ggagcaaaag
     4021 atttcctaac ggaagtattt atgatgtaaa ggtacttagg aaatgataaa acaaaatagt
     4081 tttgttccgt atcctgaagc aatgcttcct aaaggattta aatatccgca aagttattta
     4141 aaattagctc aatccactca tgccattaac tacgatgaac aatattcttt tccttggtgg
     4201 tttgaaaatg cagaaagcaa tatatcagaa gtaattgaca tttattttga aataactggc
     4261 attccaaacc tattaccttt tgctagaaac caagagtggg ctgcctgttt tgatatttca
     4321 gataaatcag gtaatcctaa aattatagta gttaatttag ataatacaaa atattacgag
     4381 acttttgaaa attttgatac ttggctaaaa gaagctgaaa atgatggttg gtagcagccg
     4441 taggtcggat tctcgaatcc gacattttcc aacagcggca tttcggaaac gatagatgcg
     4501 tcaaatattt ttgtcggatg caaatatccg acctacatct ctgcgcagca gactctacaa
     4561 ggtattaatg atttaggaaa tttaagtccg gaagcacaac ttgccgccgt gagcctatta
     4621 caggacagtg cttttgcggt aaaagacggc attaattccg ccagacaatg ggctgatgcc
     4681 catccgaata taactgcaac agcccaaact gccctttccg cagcagaggc cgcaggtacg
     4741 gtttggggcg gtaaaaaagt agaacttaac ccgaccaaat gggattgggt taaaaatacc
     4801 ggctataaaa cacctactgc ccgccctatg cagactttag atggggagat ggcaggtggg
     4861 aataagccac caaaaccaag tacgcagcaa caccctacac actctgataa caatatcggc
     4921 ttacctgcct catatgttaa acctgataca tctatttctc cgacaggaac aattcaagac
     4981 cgcatcagat ggacaaagtc caagtttcct actgagaaat ctttaaatgg acatttcaaa
     5041 gctcatggaa aagaatttgg cgatataacc attgaagact accaaaaaat ggcgtctgat
     5101 ttgttattaa aacagacatc ggacaagata ttaggttatc agacggaaca tagacgagtg
     5161 cgctatgata tcaataacaa tatctatgtt ttggccaatc caaaaacatt caaaatcaaa
     5221 acaatgttta aaccaaactt aggaaagaag tattatgatg gagaattcaa aaaagacatg
     5281 ggaaattgac ggagaaatat ggctacattg tcctgtttgc ggaactgaag ttatggacta
     5341 tgatatctgt gacgtttgtc agtggcaaaa tacaggagaa actaatatag atggtggccc
     5401 taatgaaatg acacttgcgg aggcgaaaga agcttacgca aaaggcttac caatcagata
     5461 aataagcacc tagagaaatc aatgatgacg gaatcccatg gttacctatc aaataattta
     5521 cctgttaaaa tcataaatga tattatttat gcaacccagt tagtcgaaga tttggtttta
     5581 ggaaaaataa aaattgttga ttttttaaaa tcatataaca atttttattg ttggcttggt
     5641 tttgatgagt tgcctcaatc tgagaaaata aaattcctaa gctatcttaa tatattaagt
     5701 attcataaag aaatacaaga tgaaactgtg aatagggttt ataccgattg aaaaatagta
     5761 gatagagatt aacatgttaa atgaaatttt tgaaatttat tcgagacaag gggaatcttt
     5821 gataggaatt ggaattagag aagccgcatt acccgtccct attgcaatag atatattaaa
     5881 tttatttatc aatgagagaa tacttgtatt ggggggagat atttatatca agaaagataa
     5941 ttatttttat caaacatatg ataattggta ttacgaggga agtaatttat ttaacagtat
     6001 cgacaaagca atgcattatt tatctcaaat aaaattagag aatgcatacg tatcttttgt
     6061 gttgaaattt atctaacaaa ggaagcacaa gaatagattt atagtaaaac atcaagatgt
     6121 tgaaaatgct ggcttttaat ccaacctaca ctgaccggct cagatacagc cgctcagcag
     6181 actctacaag gtattaatga tttaggaaaa ttaagtccgg aagcacaact tgctgccgcg
     6241 agcctattac aggacagtgc ttttgcggta aaagacggca tcaattccgc cagacaatgg
     6301 gctgatgccc atccgaacat tacagctact gcccaaactg cccttgccgt agcagaggcc
     6361 gcaggtacgg tttggcgcgg taaaaaagta gaacttaacc cgactaaatg ggattgggtt
     6421 aaaaatacca gctataaaaa acctgctgcc cgccctatgc agactgtaga cggggaaatg
     6481 gctgggggaa acaagccagt tgttaaatct atcagaccaa ctacgcgaga tgaattacgt
     6541 caagcattgc aagaacaagg ttttagacgt actggttcag atgaggctca atatgaaaca
     6601 tggaaaggtc ctgatggcgt gaaaatagat attcgtccaa atggagaggt tataagaacc
     6661 caaagagtgc cgcgaaccga tggtgtacag ggaaaatatc cgcaacgaca aaattatgaa
     6721 ggcaatccat tgccaaataa tcgtcatcat tctggatatt ttatcaaatg aaaaaaaata
     6781 tttttcacaa tgtaagcctt tatgaaataa tcttttccga taatggaaat acccttacat
     6841 tatcttttac agatacaatt gaaggtaatt atttcggata tatcaaatgc agtaatattt
     6901 tgaattttaa attagataca aataatttcg tagattatga ggataaggaa gatagcttgt
     6961 ttcccttgtt tatacccgaa atagagctat ataaatacca attttatagt gaaattatta
     7021 ttgatgtagg gattattata aaaatatctg ctgaaacaat taattttgag ccactgggaa
     7081 aatagtaact gctttcccag cagccgtagc aactgtattt ttacccgacg gggtaaaaat
     7141 acagttgcta catctctgcg cagcagactc tacaaggtat taataattta ggaaaattaa
     7201 gcccggaagc acaacttgct gccgcgagca tattacagga cagtgctttt gcggtaaaag
     7261 acggcatcaa ttccgccaga caatgggctg atgcccatcc gaatataaca gcaacagccc
     7321 aaactgccct tgccgtagca gaggccgcag gtacggtttg gagaggtaaa aaagtagaac
     7381 ttaacccgac caaatgggat tgggttaaaa ataccggcta taaaaaacct gctgttcgcc
     7441 atatgcagac taaggcgtta ggtacggtag atgaaattgg cgatacagta cagcaggttg
     7501 ggaaacaggc tagcggacaa aaaaccagcg gtggtaatcc tgcgattgat agcgacccct
     7561 atagcccgag tagtgtggca gctcgcatag aagccggtaa ggcgcgcagt gatttacaaa
     7621 tcaaagacat tttgagcaat actactcaaa ggaggtcccg ctgttcagta tgataaagtg
     7681 ggggattaca atgacgcact aaatgatttt aatagtctga atgttcgaaa tgtacaaaca
     7741 cgtcctaatg gaacgataac gggcaattta cctgatgggc gtgcggttaa tgctcgtaat
     7801 gatagtagtg gtggagaacc aacacttgaa ataacaatta gtaataaccg aaaaataaaa
     7861 atcagatatg gaaatacacg ataaattatg aaattaaaaa gcttagattt cccaactggc
     7921 tatttctatt ttgataatgc agcaataaac tctgataaag tagaagttat agcagttggt
     7981 tatagaaata cggataaaac cataaaaatt tttattgaag atgttattca ttttagggtt
     8041 gttgatgaat cgtattttat agatactttt atggatttaa tttcggaaga tgcagataga
     8101 gctttgcttc atgaaaatgg tggtcaatct ttttttgaac ttcttgatga gtgttatgcg
     8161 gaatggatat tgaaagaaag ttattttcct ttgaatagag aattctttaa atactatatt
     8221 tttatgtttg agcaaacatt catagaaata attggttcta gtgcaacgta ttcaattatt
     8281 gagggctagc ataagatgag taataagttg cctatctttc tttcaggcag cctgaaaata
     8341 aaactaccca agttgatggt gtacctgtat cagtgaaggg aaattttgtt gatggtaaat
     8401 ttcgcattgg tacggcaaca atgaaatcat tttaaattga gctagaaatg aacctagaaa
     8461 attatgaaaa cattttaata aaattacttt tttatcataa caacttagta aatgaatatt
     8521 cttattttat tgaaaataaa aatttattta aaatagtatc tcgaacgaaa acgagtaagg
     8581 gctttttttt cactatagaa aaaccattaa attttctaag caaaaaaact tattttgagt
     8641 ttaattttaa atatttacac tcagggaaag aacgctttgg ttcgtttatg tgctggataa
     8701 atactaattt aatggaattt gagggggttt ttttaacgac ctgctccctg ataatatgat
     8761 aataaataac ttttttgaaa taaatgatta acgatacacc aataaaaatt ggtggggtaa
     8821 ccgtatggat gatattcgcg gcatcatcca aggtgcggtt aatcctttaa tttacaaggt
     8881 aagcaagcaa aagattattt acaacaacaa actcatatca ggaacttaga caaatgaatg
     8941 aacacaacct gttaattttc tgtttaaaag acaatgtttc aattagtgaa tatactgaaa
     9001 tgattgattg ggcttataaa aacattcaat ctgaaacagt tgtagaaatt acggaaaatc
     9061 aaattattga atatcaaaat cgtggattat ggagacttgt ttctgaaatt accgataatt
     9121 ggttatttgg accaagtgag ggggattggc taatagataa ggaaagtatt ttggctgtaa
     9181 aagaaaaatt acaaaattca gatttttcta cagagtcctt agtgaaaaat attattcatg
     9241 cacttgaata tgctataaaa aatgaaaaaa cagtaatttt tcatttttga gactaatcca
     9301 atttttagta atattgatgc agagcaagca gcattagatg ccgcaaacat ggggagaagc
     9361 tattcaattt agaattaaaa aacaaattga aaatgaacta gcaccaccaa attggtctac
     9421 ccagtttcct aatggtagta tttatgatcc taaggtaacg aaatgattat tcaaaatgaa
     9481 tttaatttat atcctagtaa tatgcttcct gaaaggtttt gttatcctga aaagtatgtt
     9541 cgtatctcta acgatacatc tttaatacct tatattcagc cacataattt tcactggtgg
     9601 tttgagaatt atggaacaga aggggcagaa gtagcttata tatttagaaa ttctatcctg
     9661 cctgatttaa atcttatccc attcgctagt aatggagaat gggaagctta ttttgatggt
     9721 aatgatgtaa caggaaatcc tagggttatt gtcattaatt tagataatat agaaaaccat
     9781 gaatttttta atagttttga agagtggctt gaattagcaa ttaaggatac ttggtaagca
     9841 gctatctata aaagagatgg gaccatcctg gataactagg ataaactcg
//
LOCUS       DAWKQB010000063         9844 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.063, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000063 DAWKQB010000000
VERSION     DAWKQB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.063"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            31..855
                     /locus_tag="WJL14_001191"
     CDS             31..855
                     /locus_tag="WJL14_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656260.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAVLADALTAPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQVYKQSHSALTALQTEQVQDSEHSGKMVAKRQFRIGDIAGEHTSF
                     DKLPEGGRATYRGTAFGSDDASGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAASDIK
                     PDKKRHAVISGSVLYNQAEKGSYSLGIFGGQAQEVAGSAEVETANGIRHIGLAAKQ"
     gene            995..1672
                     /gene="tsaB"
                     /locus_tag="WJL14_001192"
     CDS             995..1672
                     /gene="tsaB"
                     /locus_tag="WJL14_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ7656261.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETCLFHQEVGSRQSALI
                     LPEIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGVPSLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGNAFALENRPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            1669..2109
                     /gene="rimI"
                     /locus_tag="WJL14_001193"
     CDS             1669..2109
                     /gene="rimI"
                     /locus_tag="WJL14_001193"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ7656262.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFTHLPEDTQRLLLE
                     VRAGNTAAQALYTAHGFSITGRRKNYYRTADGKTEDAVLMEKIC"
     gene            2103..2840
                     /locus_tag="WJL14_001194"
     CDS             2103..2840
                     /locus_tag="WJL14_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ7656263.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPAPSAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFANPERQAMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            2902..3543
                     /gene="pyrE"
                     /locus_tag="WJL14_001195"
     CDS             2902..3543
                     /gene="pyrE"
                     /locus_tag="WJL14_001195"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ7656264.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            3617..4051
                     /locus_tag="WJL14_001196"
     CDS             3617..4051
                     /locus_tag="WJL14_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656265.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGMFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTSAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            4048..5358
                     /gene="argA"
                     /locus_tag="WJL14_001197"
     CDS             4048..5358
                     /gene="argA"
                     /locus_tag="WJL14_001197"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ7656266.1"
                     /translation="MSAPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLISGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            5454..7469
                     /locus_tag="WJL14_001198"
     CDS             5454..7469
                     /locus_tag="WJL14_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ7656267.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPGWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWGERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGIPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKCRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(7583..8539)
                     /locus_tag="WJL14_001199"
     CDS             complement(7583..8539)
                     /locus_tag="WJL14_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656268.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            8775..9740
                     /locus_tag="WJL14_001200"
     CDS             8775..9740
                     /locus_tag="WJL14_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7656269.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         2168 a         2992 c         2777 g         1907 t
ORIGIN      
        1 tctattctgc gtatgactag gagcaaacct gtgaaccgaa ctgccttctg ctgcctttct
       61 ttgaccgccg ccctgattct gaccgcctgc agcagcggag gcggcggtgt cgccgccgac
      121 atcggcgcgg tgcttgccga tgcactaacc gcaccgctcg accataaaga caaaagtttg
      181 cagtctttga cgctggatca gtccgtcagg aaaaacgaga aactgaagct ggcggcacaa
      241 ggtgcggaaa aaacttatgg aaacggcgac agcctcaata cgggcaaatt gaagaacgac
      301 aaggtcagcc gcttcgactt tatccgtcaa atcgaagtgg acgggcagct cattaccttg
      361 gagagcggag agttccaagt gtacaaacaa agccattccg ccttaaccgc ccttcagacc
      421 gagcaagtac aagattcgga gcattcaggg aagatggttg cgaaacgcca gttcagaatc
      481 ggcgatatag cgggtgaaca tacatctttt gacaagcttc ccgaaggcgg cagggcgaca
      541 tatcgcggga cggcattcgg ttcagacgat gccagtggaa aactgaccta caccatagat
      601 ttcgccgcca agcagggaca cggcaaaatc gaacatttga aatcgccaga actcaatgtt
      661 gacctggccg cctccgatat caagccggat aaaaaacgcc atgccgtcat cagcggttcc
      721 gtcctttaca accaagccga gaaaggcagt tactctctag gcatctttgg cgggcaagcc
      781 caggaagttg ccggcagcgc agaagtggaa accgcaaacg gcatacgcca tatcggtctt
      841 gccgccaagc agtaaccatt gtgaaaatgc cgtccgaaca cgataattta ccgttcggac
      901 ggcatttttg cgccataccg ccctaggata cgacggcacg gcaggcaagc cggtttcact
      961 atataatgcc gtctgaacca acgagaacca caccatgcaa gccgatttca accgccccgt
     1021 cctcgccgtc gataccggaa cttcctattt gtcgctcgcg ctgcgcgccg acggcgaaac
     1081 ctgcctgttc catcaggaag tcggcagccg ccagtccgca ctgattctgc cggaaatccg
     1141 cacgttgttt cacgatgcag gcattaccgc cgccgatttg ggggcgattg tttatgcgaa
     1201 aggccccggc gcgtttacgg gactgcgtat cggcatcggc gtggcgcaag gtttggcaac
     1261 gccgtttgat actcctttaa tcggcgtacc ctcgctcgat gccgccgcct cgctgccgcc
     1321 gccgcaaagc tgcatccttg ccgctgcgga cgcacgtatg ggcgaagtgt tttatgcatg
     1381 gttcgacacg ctgaaccgcc gccgtttgag cgattatcag gtcggtcggg cggcagacat
     1441 ccggctgccg gaaggatgcg ccttttcaga cggcatcggc aatgcgttcg cgctggaaaa
     1501 ccgtccgccc ttctcaggca aacccgatat gccgaccgcc gccgacttcc tcaatctggc
     1561 ggctgaaggc ggttttcccg ccgtcgctgc ggcacacgcc gagctgctct acgtccgcaa
     1621 caaaatcgcc ttgactgcca aagaacaggc ggaacggagg gcgcgcctgt gaacatccgc
     1681 cgcgccgttt atgccgattg cgccgcactc gccgcgctcg atgccgtctg caacccgtcc
     1741 gcgtggacgc aacgccaatt tgagtccgca ctggtttcgc cgtccgaaca ggttttcctt
     1801 gcggaaaaag acggcgggat tgccgccttt atcgtttggc agaacctgcc cgacgaatcc
     1861 gaactgcacc tgattgccac cgcgcccgaa tgccgccgcc aaggaattgc gtccgccctg
     1921 ctcgaatatt ggttcacaca tctgcccgaa gacacgcaac gcctgctgct cgaagtccgt
     1981 gcaggcaaca ccgccgcaca ggcactgtac acggcgcacg gcttcagcat tacgggcagg
     2041 cggaaaaact attaccgtac agccgacggt aaaaccgaag atgccgtctt aatggagaaa
     2101 atatgttaag cgcgcgctac ctccacctgc acgaagcttt gggtttgggt ccgatgtggc
     2161 tgaaacaggc cgccgccgtc ctgccgccca aaaacacacc cgcaccctcg gcacaggcac
     2221 gtccccaaac cgtccgcgcc gccccgatcc gcccttccca accccataac ggtcaggcgc
     2281 ggctcgaaac gatgaaagcg ttggaaaccg ccgccgtaca tacgcgcaaa cccgcgcctg
     2341 aaaccgaaac gcccccgccc ggcctttcag acggcatcgc ccccgttccc gccgcttcgg
     2401 gcataaccaa acttgccgtc gtcagccttt gcccaccgat tgaggatgcg gtttacgggc
     2461 aactgttcta cggcaaagcg ggtgttctgc ttgacaacat actcaaagcc gtaggactgg
     2521 atgccgccta tgtccacaaa acctgttggg tgaaaaccgc cgccgtcggc aacccgatgc
     2581 cgtctgaagc ggcaatcgaa accgcgacgg tttccgtcat gcaggaactc gacggctgcc
     2641 gcgcccccgc cgtcctattc ctcggacagg cgtttgccaa cccggaacgg caggcgatga
     2701 ttgaaacttt gtgcggcagc cgtcccttct tcatcatcga ccatcccgcc cggcttttac
     2761 gccaacccga actcaaagcc cgcgcctggc aggtgttgaa acagttgaaa cgcgccttgc
     2821 ggcaaggcgg cggcagttga agcgcaccgc acggggcggt agaatcgcaa ctgcgtccca
     2881 atatctgaca gaaagcacaa aatgaccgat ttccgccaag atttcctcaa attctccctc
     2941 gcccaaaatg ttttgaaatt cggcgaattc accaccaaag ccggacggcg gtcgccctat
     3001 ttcttcaatg ccggcctctt taacgacggc ttgtccacgc tgcaactggc aaaattttac
     3061 gcacaatcca tcattgaaag cggcatccga ttcgatatgc tgttcggccc cgcctacaaa
     3121 ggcattattt tggcggcggc aaccgcgatg atgctggcgg aaaaaggcgt gaacgtcccg
     3181 tttgcctaca accgcaaaga agccaaagac cacggcgaag gcggcgtgtt ggtcggcgcg
     3241 ccgcttaaag ggcgcgtgct gattatcgac gacgtgattt ccgccggcac atccgtacgc
     3301 gaatcgatca aactgattga agcggagggt gcaacccccg ccggtgtcgc catcgcgctc
     3361 gaccgcatgg aaaaaggcac gggtgaattg agcgcggttc aggaagtgga aaaacaatac
     3421 ggcctgcccg tcgcccccat cgccagcctg aacgatttgt ttattctgtt gcaaaacaac
     3481 cccgaattcg gacagttcct cgaacccgtc cgagcctacc gtcggcagta cggcgtagaa
     3541 taaaaacaaa gcatatgccg tctgaaccgc cttacgcctc agacggcatc aaacctgaca
     3601 cacacgagga aataccatgc ccgcctgttt ctgcccccac tgcaaaaccc gtctctgggt
     3661 caaagaaacc caactcaatg tcgcccaagg cttcgtcgtc tgccaaaaat gcgaaggaat
     3721 gtttaaagcc aaagaccatc tggcaagcac gaaagaaccc atattcaacg atttgcccga
     3781 agctgtttcg gatgtcaaac tcgttcaccg catcggcacg agcgccatcg gcaagaaaca
     3841 gatttcccgt gacgaaatcg ccggcatcct caacggcggc acaacccagc ccgatattcc
     3901 gcccgcaacc gccgccaccc ctgctgccgc accgcaggtt accgtaccgc ccgccgcgcc
     3961 cgcccgtcag gatgggttca actggacgat tgcaaccctg tttgccctta tcgtcctcat
     4021 tatgcagctt tcctacctcg tcatcctatg agcgcgcccg acctctttgt cgcccacttc
     4081 cgcgaagccg ccccctacat ccgccaaatg cgcggcaaaa cgctggtcgc cggcatagac
     4141 gaccgcctgc tcgaaggtga taccttaaac aagctcgccg ccgacatcgg gcttttgtcg
     4201 caactgggca tcaggctcgt cctcatccac ggcgcgcgcc acttcctcga ccgccacgcc
     4261 gccgcgcaag gccgcacgcc gcattattgc cggggcttgc gcgttaccga cgaaacctcg
     4321 ctcgaacagg cgcagcagtt tgccggcacc gtccgcagcc gttttgaagc cgcattgtgc
     4381 ggcagcgttt ccgggttcgc gcgcgcgcct tccgtcccgc tcatatcggg caacttcctg
     4441 accgcccgtc cgataggtgt gattgacgga accgatatgg agtacgcggg cgttatccgc
     4501 aaaaccgaca ccgccgccct ccgtttccaa ctcgacgcgg gcaatatcgt ctggctgccg
     4561 ccgctcggac attcctacgg cggcaagacc ttctatctcg atatgcttca aaccgccgcc
     4621 tccgtcgccg tctcgcttca ggccgaaaaa ctcgtttacc tgaccctttc agacggcatt
     4681 tcccgccccg acggcacgct cgccgaaacc ctctcggcac aggaagcgca atcgctggcg
     4741 gaacacgccg gcggcgaaac gcgacggctg atttcgtcca ccgttgccgc gctcgaaggc
     4801 ggcgtgcatc gcgtccaaat cctcaacggt gcctccgacg gcagcctgct gcaagaactc
     4861 ttcacccgca acggcatcgg cacatccatt gccaaagaag ccttcgtctc catccggcag
     4921 gcgcacagcg gcgacatccc gcacatcgcc gccctcatcc gcccgctgga agaacagggc
     4981 atcctgctgc accgcagccg cgaatacctc gaaaaccaca tttccgaatt ttccatcctc
     5041 gaacacgacg gcaacctgta cggttgcgcc gccctgaaaa cctttgccga agccgattgc
     5101 ggcgaaatcg cctgccttgc cgtctcgccg caggcacagg acggcggcta cggcgaacgc
     5161 ctgcttgccc acattatcga taaggcgcgc ggcataggca taagcaggct gttcgcactg
     5221 tccacaaata ccggcgaatg gtttgccgaa cgcggctttc agacggcatc ggaagacgag
     5281 ttgcccgaaa cgcggcgcaa agactaccgc agcaacggac gaaactcaca tattctggta
     5341 cgtcgcctgc accgctgacc gcaacggaaa gccgccgcag aaatgccgtc tgaaccccgt
     5401 ttcagacggc atttccccga ttataaagac ctgcccaacc ctcaaggacc ccgatgaaat
     5461 cctaccccga cccctaccgc cattttgaaa acctcgattc cgccgaaacg caaaacttcg
     5521 ctgctgaagc gaatgccgaa acgcgcgcgc gttttttaaa caacgacaag gcgcgcgcgc
     5581 tttcagacgg cattttgaat caaatgcagg acacgcggca gattccgttt tgtcaggaac
     5641 accgcgcgcg gatgtaccat ttccatcagg acgcagaata tccgaagggc gtgtaccgcg
     5701 tgtgtaccgc ggcgacatac cgttccggct atcccgggtg gaaaatcctg ttttcagtgg
     5761 cggatttcga cgaattgctc ggcgacgatg tatatttggg cggcgtgtcg cacttggtgg
     5821 aacagcccaa ccgtgcgttg ttaacactga gcaaatcggg cggcgatacg gcgtacacgc
     5881 tggaagtgga tttggaagca ggggagttgg tagaaggcgg ttttcacttt ccggcaggca
     5941 aaaaccatgt gtcgtggcgc gatgaaaaca gcgtgtgggt gtgtccggct tggggcgaac
     6001 gccagttgac cgaatcgggc tatccgcgcg aggtgtggct ggtggaacgc ggcaagagtt
     6061 tcgaggaaag cctgcctgtg tatcaaatcg ataaaggtgc aatgatggtg aacgcgtggc
     6121 gttatctcga tccgcaaggt tcgccgattg atttgattga agcgtcggac ggtttttaca
     6181 ccaaaaccta tttgcaggtg tcgtcggaag gcgaggcgaa accgttaaac ctgcccaacg
     6241 attgcgacgt ggccggctat ctagccggac atcttttgct gacattgcgt aaagactggc
     6301 accgcgcgaa ccaaagctat ccgagcggcg cattggtggc agtaaaatta aaccgaggcg
     6361 aactcggggc ggcgcagctt ttgtttgcgc ccgatgaaac gcaggcattg gaaagcgtgg
     6421 aaacgaccaa gcgttttgtg gtggcgagcc tgctggaaaa cgtacaaggc cgtctgaaag
     6481 cgtggcggtt tgccgacggc aaatggcagg aagtcgaact accgcgcctg ccttcgggcg
     6541 cgttggaaat gaccgaccag ccatggggcg gcgatgtggt ttaccttgcc gccagtgatt
     6601 tcactacgcc gctgacgctg tttgcgctgg atttgaacgt gatggaactg accgtcatgc
     6661 gccgtcagcc gcagcagttc gattcagacg gcatcaacgt gcagcagttt tggacgactt
     6721 cggccgacgg cgagcgtatt ccttattttc acgtcggcaa aaacgccgcc cccgacacgc
     6781 cgactttggt ttatgcatac ggcggttttg gcattcccga gttgccgcat tatttgggca
     6841 gcgtcggcaa atattggctg gaagagggca atgcctttgt gttggcaaac atccgcggcg
     6901 gcggcgagtt cggcccgcgc tggcatcagg cggcgcaggg aatcagcaaa cacaaaagcg
     6961 tcgacgattt gcttgccgtc gtgcgcgatt tgtccgaacg cggtatcagt tcgccccaac
     7021 acatcggctt gcagggcggc agcaacggcg gcctgattac cgccgccgcc ttcgtgcgcg
     7081 aaccgcaaag catcggcgcg ctggtgtgcg aagtgccgct gaccgatatg atccgctatc
     7141 cgctgctctc cgccggcgca agctggacgg acgaatacgg caacccgcag aaatacgaag
     7201 tctgcaaatg ccggttgggc gaattgtcgc cgtatcacaa tctttcagac ggcatcgatt
     7261 atccgccagc gctcattacc accagcctca gcgacgaccg cgtccatcct gcccacgcgc
     7321 tcaagttcta cgccaaactg cgcgaaacct ccgcgcaatc ttggctctac tcacctgacg
     7381 gcggcggcca taccggcaac ggcacccaac gcgaatccgc cgacgaactc gcctgcgtgt
     7441 tgctgttttt gaaagagttt ttgggctaag ggcggggagc ggcactgccg ccgcgaatga
     7501 aaaaaggtcg tctgaaactg ctttttcaga cgaccttttt taatggttgt ttcaaatcaa
     7561 aatatctatg ccgccggccc catcagcact tcttcacatc cgaaggcaaa aatccgtaat
     7621 gccgtctgaa cgcttcgttg aaccgtcccg cgtggcggta gccgcaaaag tgcatggcgg
     7681 cttggacggt gctgccggat tcgatgaggg cgagcgcgtg ttccagccgc aggcggcgca
     7741 ggcatccggc gacggtttcg ccggtttgcg ctttgaaata gcgtttcagg tagcattcgt
     7801 tcagtccgac gcggcgggcg atttcggcga tggtcagcgg acgggcgaat tcgtgttgca
     7861 ggatgtcggc ggcttcgtct atgcgccgac ggcggtaacc gttgtcgtgg cggcggaagg
     7921 tgaagcgcaa taatcgggcg gagagttcca gcgcggcggc ttcgtcggca agcaggccga
     7981 agccgtccga ttcgaacgag cgttgcagca gtgggcaggc cgccgccgtc agtgccgctg
     8041 cgttttgcgc cagccgttgc agggcgaatc ggcctatcgt ttgcggcgaa aacaggcgtt
     8101 cgtccagcaa gccttcgtcg tgccagcggc gcagtttttc cagcgaaaaa tccaaatgca
     8161 gcgcgcacat gccgctgttg tcgggcagca gggtttcgga tacgtccgcc aactcgccgc
     8221 gtaccagcca gatttcgccg gcagacgggc ggtattccct gccgcccatt tgtaaccggt
     8281 tctgccccga caccatgacg aacaaggcgc agttgtggct gaaattgtgg atttcggtgg
     8341 gaaacgcgcc cgttccgccg ccgcgcatcc gcgacaaggt gatgcccgag tcgaagcggt
     8401 tgatgcacat ttccagatgc aaaccgggct gttttgcctg cgcaatgagc gcgctgtcgg
     8461 aacagccgtc caacgcccag ccggatttat cggagcggac ataggtttgg tactggcggt
     8521 agatggcggc ggtgttcatg attggatagg aacgagttgt ctaacaaatg aattaaatag
     8581 gaattattac caataatcaa gcgcagggat tggttgaaac ggaaaaggtc gtctgaaagg
     8641 gtgtttcaga cgaccttttc cgtatcggga atttgttttg ccgtatcggg aattttgcgt
     8701 tttgcggcgt ggtttctgca ggttgtttgc ttaataataa acattcttat tcgtatgcaa
     8761 aggaaccgca caccgtgaaa ccgcgttttt attgggcagc ctgcgccgtc ctgctgaccg
     8821 cctgttcgcc cgaacctgcc gccgaaaaaa ctgtatccgc cgcatccgca tctgccgcca
     8881 cactgaccgt gccgaccgcg cggggcgatg ccgttgtgcc gaagaatccc gaacgcgtcg
     8941 ccgtgtacga ctgggcggcg ttggatacgc tgaccgaatt gggtgtgaat gtgggcgcaa
     9001 ccaccgcgcc ggtgcgcgtg gattatttgc agcctgcatt tgacaaggcg gcaacggtgg
     9061 ggacgctgtt cgagcccgat tacgaagccc tgcaccgcta caatcctcag cttgtcatta
     9121 ccggcgggcc gggcgcggaa gcgtatgaac agttggcgaa aaacgcgacc accatagatc
     9181 tgacggtgga caacggcaat atccgcacca gcggcgaaaa gcagatggag accttggcgc
     9241 ggattttcgg caaggaagcg cgcgcggcgg aattgaaggc gcagattgac gcgctgttcg
     9301 cccaaacgcg cgaagccgcc aaaggcaaag gacgcgggct ggtgctgtcg gttacgggca
     9361 acaaggtgtc cgccttcggc acgcagtcgc ggttggcaag ttggatacac ggcgacatcg
     9421 gcctaccgcc tgtagacgaa tctttacgca acgaggggca cgggcagcct gtttccttcg
     9481 aatacatcaa agagaaaaac cccgattgga ttttcatcat cgaccgtacc gccgccatcg
     9541 ggcaggaagg gccggcggct gtcgaagtat tggataacgc gctggtacgc ggcacgaacg
     9601 cttggaagcg caagcaaatc atcgtcatgc ctgccgcgaa ctacattgtc gcgggcggct
     9661 cgcggcagtt gattcaggcg gcggagcagt tgaaggaggc gtttgaaaag gcagaacccg
     9721 ttgcggcggg gaaagagtag ggagtcgtct gaaaacggag cttccgaagg aagcgggggg
     9781 tttctgcgaa gctaaagtgc ggtttcaacg aattgaaaag cagcctgtat gttgaaaata
     9841 ccgc
//
LOCUS       DAWKQB010000064         9827 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.064, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000064 DAWKQB010000000
VERSION     DAWKQB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.064"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..557
                     /locus_tag="WJL14_001201"
     CDS             30..557
                     /locus_tag="WJL14_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ7656270.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAESSRG"
     gene            645..1700
                     /gene="plsX"
                     /locus_tag="WJL14_001202"
     CDS             645..1700
                     /gene="plsX"
                     /locus_tag="WJL14_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ7656271.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVHLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAVNQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNSIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSASLSKIEQGVAEQL
                     AALETAKAVQNENVGGL"
     gene            1836..2087
                     /locus_tag="WJL14_001203"
     CDS             1836..2087
                     /locus_tag="WJL14_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ7656272.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            2338..2529
                     /locus_tag="WJL14_001204"
     CDS             2338..2529
                     /locus_tag="WJL14_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656273.1"
                     /translation="MIQTILEDGDIEVKNLKTENYSGKAREILGDSYPIEHISKNIST
                     IQNIISEFMIQKQTPAPII"
     gene            2549..2752
                     /locus_tag="WJL14_001205"
     CDS             2549..2752
                     /locus_tag="WJL14_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656274.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            2779..3741
                     /locus_tag="WJL14_001206"
     CDS             2779..3741
                     /locus_tag="WJL14_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ7656275.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            3788..4159
                     /locus_tag="WJL14_001207"
     CDS             3788..4159
                     /locus_tag="WJL14_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ7656276.1"
                     /translation="MKRQAFFKPIACAAFLSAVSLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TCILIDYFVPPELFVKLGQHL"
     gene            4185..5111
                     /gene="fabD"
                     /locus_tag="WJL14_001208"
     CDS             4185..5111
                     /gene="fabD"
                     /locus_tag="WJL14_001208"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ7656277.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFANAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQVAAFIEAH"
     gene            5250..7115
                     /gene="msbA"
                     /locus_tag="WJL14_001209"
     CDS             5250..7115
                     /gene="msbA"
                     /locus_tag="WJL14_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ7656278.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIIAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            7215..8780
                     /gene="guaA"
                     /locus_tag="WJL14_001210"
     CDS             7215..8780
                     /gene="guaA"
                     /locus_tag="WJL14_001210"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ7656279.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            8842..9588
                     /gene="fabG"
                     /locus_tag="WJL14_001211"
     CDS             8842..9588
                     /gene="fabG"
                     /locus_tag="WJL14_001211"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ7656280.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRALDSAEPETIESLIADIEKAFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRSGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFAKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQAFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         2501 a         3037 c         2344 g         1945 t
ORIGIN      
        1 caaaagcccc aaccaccaag gaaattccga tgaaacagaa aatctggtac acctacgatg
       61 acatccaccg cgtcatcaaa gcattggcag aaaaaatccg gaacgccgac atcaaatacg
      121 atgccatgat tgccatcggc ggcggcggct ttattccggc acgtatgctg cgctgttttc
      181 tggaaattcc gatttatgcc gtaaccaccg cctattacga cagcgacaac gaaggacagg
      241 ttaccgaaga agtcaaaaaa gtccaatggc tcgaccccgt tcccgaagcc ctgcggggca
      301 aaaacgtcct tgttgtcgat gaagtggacg acagccgcgt aaccatggaa ttttgcctga
      361 cagagctgct caaagaagac ttcggcacca tcggcgtcgc cgtactgcac gaaaaaatca
      421 aagccaaagc aggtaaaatc cccgaaggca ttccctattt cagcggcatc accgtagaag
      481 actggtggat caactatccg tgggacgcac tcgacatcga cgaacacaac cgccttgccg
      541 aaagcagccg aggctgaccc tttcagacgg catattttcc gaaccgatgc cgtctgaagc
      601 acgcacgacc cctgccgcag accgaaaacc taccggagaa ccctatgatt acacttgccg
      661 tagatgccat gggcggcgac caaggacttg ccgttaccgt acccggcgca accgcattcc
      721 tccaagcaca ccccgatgtc cacctgatta tgaccggcga cgaaacgcaa ctgcgccaag
      781 ccctgaccgc ggcaggcgca ccgatggaac gcatcgacat ctgccacacc gcacaaatcg
      841 tcggcatgga cgaagcgccg caatccgccc tgaagaacaa aaaagactcc tccatgcgcg
      901 tcgccgtcaa ccaagttaaa gaaggcaaag cccaagccgc cgtatccgca ggcaacacgg
      961 gtgcgctcat ggcaaccgca cgtttcgtcc tcaaaaccat tcccggcatc gaacgccccg
     1021 ccatcgccaa attccttcct tccgacaccg accacgttac ccttgcactc gaccttggcg
     1081 cgaacgtcga ctgcacgccc gaacagctcg cccaatttgc cgttatcggc agcgaactcg
     1141 tccacgcact ccatcctcaa aaaggacagc cgcgcgtcgg gctggtcaac gtcggcacgg
     1201 aagacatcaa aggtacggac accgtcaaac aaacctacaa actgctgcaa aacagcaaac
     1261 tcaactttat cggcaacatc gaaagcaaca gcatcctgta cggcgaagca gatgtcgtcg
     1321 tcgccgacgg ctttgtcggc aacgtcatgc tcaaaaccat cgaaggcgcg gtcaaattca
     1381 tgagcggagc catccgccgc gaattccaaa gcaacctgtt caacaaactt gccgccgttg
     1441 ccgccctacc cgccctcaaa gggctgaaaa acaaactcga cccgcgcaaa ttcaacgggg
     1501 ccatcctgct cgggctgcgc ggcatcgtga ttaaaagcca cggcggcaca gacgaaactg
     1561 gtttccgcta tgccctcgaa gaagcctacc atgaagccaa gtctgccagc ctttccaaaa
     1621 tcgaacaggg cgttgccgaa caactcgccg cactcgaaac tgccaaagcc gtccaaaacg
     1681 aaaatgtcgg cggtctgtaa cacacacgat gccgtctgaa cgcccccgcc cctttcagac
     1741 ggcatccgcc cgcaccaaac ctgcgggcgc ggacggcgat gcgcctgtcc ggcacttccc
     1801 aaatacttcc ctgtaaaata aggagtattt gaaaaatgaa gacattagaa aaacggatga
     1861 aagctctaga caaacagatg atgaagttcg gaaaatccct tgaaggcagg cttgatgccc
     1921 gtctgattga atccgcattg gattatattc attattcgga acgttttttg gcttttgaaa
     1981 tcctgtgtac ttatatcgaa gatttcgatg tccggctgac ggaacaagaa tcccgggaaa
     2041 tttcttttat caacaaggaa tttgagatag aaagcacgtc cgattaacca ataaagccaa
     2101 tgggttgata aacatgaaaa catcgacggt cgtttttggc ggatttttta tggcagacaa
     2161 cggagagcga atccaaatcc ccgttttgga aaatcctgac attagggaaa tcaatcactt
     2221 ttttccgtat caaattttga gaaaaaagcc ggcgtccttg ttttcagaat catccccgag
     2281 ccggaatttg gcgataccga attaactatc tattttgaaa aaggatatta tttgccaatg
     2341 attcaaacaa ttttagaaga cggggatatt gaagtcaaaa accttaaaac agaaaattac
     2401 agcgggaagg caagggaaat cttgggagat tcctacccta ttgagcatat ctctaaaaat
     2461 atttctacaa ttcaaaatat aatttctgaa tttatgatac aaaaacaaac accggcacca
     2521 ataatttgat ggaaaaccaa caatcaaaat gccgtctgaa gcatgtttgg ttttcagacg
     2581 gcatttcctt cgcttaaaac agcgtatcgg caaccccgcc ctgcctgtcc acggcaatct
     2641 gcatctgaaa accatctgta tcccaaacca cacccccatc cctgtttccg tcatttgcac
     2701 cctgtccgta ttgggcaatc atcggttttt cgcttacaat agccgaatct gaaccaacta
     2761 tccaaaaagg ccgttcccat gcagtatgcc aaaatttccg gcacgggcag ctatcttccc
     2821 gccaaccgcg tcagcaatga cgaccttgcc caaaaagtgg atacctcgga cgagtggatt
     2881 accgcgcgca cgggcatcaa attccgccat attgcagccg aaaatgagaa aaccagcgat
     2941 cttgccgccg aagcagcccg ccgcgcactg gatgcagccg gattagacag cggcgaaatc
     3001 gatttaatta ttgtggcgac ggcaacgccg gatatgcagt ttccttctac cgccaccatc
     3061 gtccaacaaa aactgggcat caccaacggc tgccccgcgt ttgacgtaca ggcggtgtgt
     3121 gcgggcttta tgtacgcgct gaccacggca aacgcctaca tcaaaagcgg catggcaaaa
     3181 aacgcgctgg tcatcggcgc ggaaactttc agccgcatcg tcgattggaa cgaccgcaca
     3241 acctgcgtat tgttcggcga cggcgcgggc gcggtggttt tgagcgcggc ggacaaaccg
     3301 ggcatcatcc acagcaaact caaagccgac ggcaattatc tgaaactctt aaacgtcccc
     3361 gggcaaatcg cctgcggcaa agtttccggt tcgccgtaca tttcgatgga cggtcccggc
     3421 gtgttcaagt ttgccgtcaa aatgctgtcc aaaatcgccg atgacgttat cgaagaagcc
     3481 ggttataccg ccgctcaaat cgactggatc gtgccgcatc aggcaaaccg ccgcattatc
     3541 gaatcgaccg cgaaacattt aggtttgagt atggacaaag tcgtcctgac cgtccaagac
     3601 cacggcaaca catccgccgc atcgattccg ctggctttgg atacgggcat ccgcagcgga
     3661 caaatcaaac gcggtcaaaa cctgctgctc gaaggcatcg gcggcggttt cgcgtggggc
     3721 gcggtgctgt tgcaatattg aacccgatgc cgtctgaaac aggctttcag acggcatttc
     3781 ccatatcatg aagcggcagg ctttcttcaa accaattgcg tgtgcggcat ttctgtctgc
     3841 cgtttcgctg cgcctccccg tattgggcgc gtgttacgca atattgtccc tctatgcgtt
     3901 tgcactttac ggcatcgaca aacggcgtgc cgtgcgggga aaacgccgca ttcccgaaca
     3961 ccgcctgctg ctgcctgcct tgttcggcgg ttgggcgggc gcatacttgg gcagcaggat
     4021 attcaggcat aaaacggcga aaaagcgttt tgttgtgctg ttccgtctga ctgtttcggg
     4081 caatgtcctg gcgacctgca tcctgattga ttatttcgtt ccgcccgaac tttttgtcaa
     4141 actcgggcaa catctctgac aatccaacca aacaaaggaa taccatgtct tttgcattct
     4201 tctttcccgg acaaggttct caaagcctcg gcatgatgaa cggctttaca gggcaggccg
     4261 tcgtcaaaaa cacatttgac gaagcctccg ccatattggg gcaggacttg tgggcgatga
     4321 ttaacggcag cgatgccgaa atcatcggtc aaaccgtcaa tacccaaccc atcatgctcg
     4381 ccgccggcgt tgccgtttac cgcgcctatt tggaagcggg tggcaaaacg cctgccgccg
     4441 ttgccggaca cagcctcggc gaatataccg cgcttgtcgc cgccggcgca ttggattttg
     4501 ccaacgcggt caaactcgtg cgcctacgcg cagaactgat gcagtccgcc gtaccgcaag
     4561 gcgtgggcgc aatggcggca attttgggtt tggaagatga acaggtaagg cagatttgca
     4621 ccgaatccgc acaggatgaa gtggttgaag ccgtcaactt caactcgccc ggacaagtcg
     4681 taattgcagg caatgccgcc gccgtcggac gcgccatgac cgccgccaaa gaagccggtg
     4741 ccaaacgcgc cctgccgctg cccgtgtccg taccttccca ttgcagcctc atgaaacccg
     4801 ccgccgacaa actcgccgaa gccctcaaaa ccgtcgaaat caagcagccg caaatccgcg
     4861 tcatccacaa cgccgacgtt gccgcctacg atgatgccga caaaatcaaa gacgcgctcg
     4921 tccgccagct ttacagcccc gtacgctgga cggaaaccgt caacgccctt gtttcagacg
     4981 gcattgccga atccgccgaa tgcggaccgg gcaaagtgtt ggcgggcttg gcaaaacgca
     5041 tcaacaaaac cgccgcgtgc agcgcgctga ccgatgccgg acaggttgcc gcctttatcg
     5101 aagcgcactg acttcgttcc gcaaaaagca gcctgccctc ttcaggctgc ttttcacgtc
     5161 cgaacggcaa acagccgcat atttacgcta taatccatcc cgaccaaacc accgacagcg
     5221 gctgccgttg cagtttccgc cccaccgata tgatagaaaa actgactttc ggactgttta
     5281 aaaaagaaga cgcgcgcagc tttatgcgcc tgatggcata cgtccgcccc tacaaaatcc
     5341 gcatcattgc cgccctgatt gccattttcg gcgttgccgc caccgaaagc taccttgccg
     5401 ccttcatcgc ccccctgatt aaccacggct tttccgcacc tgccgcgccg cccgagctgt
     5461 ctgccgccgc cggcatcatt tccaccctgc aaaactggcg cgaacagttt acctatatgg
     5521 tttgggggac ggaaaacaaa atctggaccg tcccgctctt cctcatcatc ctcgtcgtca
     5581 tccgtggcat ctgccgcttt accagcacct atctgatgac ttgggtctcc gtgatgacca
     5641 tcagcaaaat ccgcaaagat atgtttgcca aaatgctgac cctttcctcc cgctaccatc
     5701 aggaaacgcc gtccggcacc gtactgatga atatgctcaa cctgaccgaa cagtcggtca
     5761 gcaacgccag cgacatcttc accgtcctca cgcgcgacac gatgatcgtt accggcctga
     5821 ccatcgtcct gctttacctc aactggcagc tcagcctcat cgtcgtcctg atgttccccc
     5881 tgctctccct gctctcgcgc tactaccgcg accgtctgaa acacgtcatt tccgactcgc
     5941 aaaaaagcat aggcacgatg aacaacgtga ttgccgaaac ccatcaggga caccgcgtcg
     6001 tcaagctgtt caacgggcag gcgcaggcgg caaaccggtt cgacgcggtc aaccgcacca
     6061 tcgtccgcct cagcaaaaaa atcacgcagg caacggcggc acattccccg ttcagcgaac
     6121 tgatcgcctc gatcgccctc gccgtcgtca tcttcatcgc cctgtggcaa agccaaaacg
     6181 gctacaccac catcggcgaa tttatggcat tcatcgtcgc gatgctgcaa atgtacgccc
     6241 ccatcaaaag ccttgccaac attagcatcc ctatgcagac gatgttcctc gccgccgacg
     6301 gcgtatgtgc atttctcgac accccgcccg aacaggacaa aggcacgctc gcaccgcagc
     6361 gtgtcgaagg gcgcatcagc ttccgcaacg tcgatgtcga ataccgttcg gacggcatca
     6421 aagccctcga cggcttcaac ctcgacatca gacaaggcga acgcgtcgcc ctggtcggac
     6481 gttccggcag cggcaaatcc accgtcgtca acctgctgcc ccgctttgtc gaaccgtctg
     6541 ccggcaacat ctgcatagac ggtatcgaca tcgccgacat caaactcgac tgcctgcgcg
     6601 cccaatttgc cctcgtctcc caagacgtat tcctgtttga cgacaccctg tttgaaaacg
     6661 tccgatatag ccgtcccgac gcgggcgaag ccgaagtcct atccgccctc caagccgcta
     6721 acctgcaaag cctgatcgac gcatcaccgc tcggactgca ccagcccatc ggatcgaacg
     6781 gcagcaactt atccggcgga cagcggcaac gcgttgccat tgcccgcgcc attttgaaag
     6841 acgcgccgat attattattg gacgaagcca ccagcgcatt ggacaacgaa tccgaacgcc
     6901 tcgtccaaca ggcgctcgaa cgcctgatgg aaaaccgcac cggcatcatc gtcgcccacc
     6961 gcctgaccac catcgaaggg gccgaccgca tcatcgtaat ggacgacggc aaaatcatcg
     7021 aacaaggcac acacgaacaa ctgatgtccc aaaacggcta ctacacgatg ttacgcaata
     7081 tctcaaacaa agatgccgcc gtccggacgg cataaacaaa atgccgtctg aaatggtaca
     7141 atcgccccga ccctttcaga cggcatcata tccgccgacc catccgatta tcttcaatca
     7201 ctgtaaaacc cattatgacc caagacaaaa tcctcatcct tgacttcggt tcgcaagtta
     7261 cccagctcat cgcccgccgc gtgcgcgaag cccacgttta ctgcgagctg cattccttcg
     7321 atatgccttt ggacgaaatc aaagccttca accccaaagg catcatcctt tccggcggcc
     7381 ctaattccgt ttacgaatcc gactatcaag ccgataccgg tatctttgac ttgggcattc
     7441 cgattttggg catttgctac ggcatgcaat ttatggcgca ccacttgggt ggtgaagtgc
     7501 aacccggcaa ccagcgcgaa ttcggttacg cgcaagtcaa aaccatcgac agcgagctga
     7561 cacgcggcat tcaagacgat acgccaaaca cgctcgacgt atggatgagc cacggcgata
     7621 aagtttccaa actgcctacc ggtttcaccg tcatcggcga cacgccgtcc tgcccgattg
     7681 ccatgatgga aaacgccgaa aaacaattct acggcatcca attccacccc gaagtaaccc
     7741 acaccaaaca aggccgcgcc ctgttgaacc gctttgtttt ggatatttgc ggcgcacaac
     7801 cgggctggac aatgccgaac tacatcgaag aagccgttgc caaaatccgc gaacaagtcg
     7861 gcagcgacga agtgatttta ggcttgtccg gcggcgtgga ctcttccgta gccgccgcgc
     7921 tgatccaccg cgccatcggc gaccaactga cctgcgtgtt cgtcgatcac ggtttgttgc
     7981 gcctgaacga aggcaaaatg gtgatggata tgttcgcccg caacttgggt gtgaaagtga
     8041 ttcacgtcga tgccgaaggg cagtttatgg cgaaactcgc cggcgttacc gaccctgaga
     8101 aaaaacgcaa aatcatcggc gcggaattta tcgaagtatt tgatgccgaa gagaaaaaac
     8161 tcaccaacgc caaatggctg gcgcaaggca cgatttaccc cgacgtaatc gaatccgcag
     8221 gcgcgaaaac caaaaaagcc cacgccatca aatcccacca caatgtcggc ggcctgcctg
     8281 aaaacatgaa gctcaagctg cttgagccgt tgcgcgactt gtttaaagac gaagtgcgcg
     8341 aactgggcgt tgctttgggt ctgccgcgcg aaatggtgta ccgccatccg ttccccggcc
     8401 ccggtttggg cgtgcgcatc ttgggcgaag tgaaaaaaga atacgccgac ttattgcgtc
     8461 aagcagacga tattttcatc caagaattac gcaacaccac cgacgaaaac ggcacatctt
     8521 ggtacgacct gaccagccag gcatttgccg tattcctgcc tgtgaaatcc gtcggcgtaa
     8581 tgggcgacgg acgcacttac gactacgtcg tcgcactgcg cgcagtcatc accagcgact
     8641 tcatgaccgc acactgggca gaactgcctt actcactgct cggacgcgtg tccaaccgca
     8701 tcatcaacga agtcaaaggc atcaaccgcg tggtttatga tgtgagcggc aaaccgcctg
     8761 ccaccatcga gtgggaataa ccagcaaaca gggctgcacc gtccggcgca gtctttcgat
     8821 tatcggacaa aaggaaaaaa tatgagcaca caagatttaa gcggcaaaat cgctttggta
     8881 accggcgcct cgcgcggtat cggtgcagca attgccgaca cactggcggc agccggtgcc
     8941 aaagtcatcg gtacggcgac cagtgagagc ggtgcggcgg cgattagcga gcggttggcg
     9001 caatggggcg gcgaaggccg tgcattggat tccgccgaac ctgaaaccat cgaaagcctg
     9061 attgccgaca tcgaaaaagc attcggcaaa ctcgacattc tggtcaacaa cgctggcatc
     9121 acccgcgaca acctcctgat gcgtatgaaa gaagaagagt gggacgacat catgcaggtc
     9181 aacctcaaat ccgtcttccg cgcttctaaa gccgtcttgc gcggcatgat gaaacaacgt
     9241 tccggccgca tcatcaacat cacatccgtc gtcggcgtga tgggcaatgc cgggcaaacc
     9301 aactacgccg ccgccaaagc aggcttaatc ggttttgcca aatccatggc gcgcgaagtc
     9361 ggcagccggg gcattaccgt caactgcgtc gcccccggct ttatcgatac cgatatgacc
     9421 cgcgccctgc cggaagaaac ccgccaagcc tttaccgccc aaaccgcctt gggcagattc
     9481 ggcgacgcgc aagacattgc cgatgcagtc ttgttcttgg cttccgatca ggcaaaatac
     9541 attaccggtc aaacgctgca cgtcaacggc ggcatgctga tgccctgacg ggcagccgta
     9601 ccgacaatgc cgtctgaagc cctttcagac ggcatttgca ttctcaggca aaatgaacac
     9661 acgccacacc ccgccctgcc cctgcggatc aggcacaagc tgagaccttt gcaaaactcc
     9721 tttccctccc gacagccgaa acccaaacac aggttttcgt ctattttcgc cccaaatatc
     9781 tcctaatttt acccaaatac ccccttaatc ctccccggat acccgat
//
LOCUS       DAWKQB010000065         9797 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.065, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000065 DAWKQB010000000
VERSION     DAWKQB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.065"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(13..1308)
                     /locus_tag="WJL14_001212"
     CDS             complement(13..1308)
                     /locus_tag="WJL14_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ7656281.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WJL14_001213"
     CDS             complement(1434..2321)
                     /locus_tag="WJL14_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7656282.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIVSGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WJL14_001214"
     CDS             complement(2321..3286)
                     /locus_tag="WJL14_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ7656283.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WJL14_001215"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WJL14_001215"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656284.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLGNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="WJL14_001216"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="WJL14_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WJL14_001217"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WJL14_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ7656285.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WJL14_001218"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WJL14_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ7656286.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WJL14_001219"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WJL14_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ7656287.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQFKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WJL14_001220"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WJL14_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ7656288.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAISAKVD"
     gene            8697..>9797
                     /locus_tag="WJL14_001221"
     CDS             8697..>9797
                     /locus_tag="WJL14_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ7656289.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCQPQKGKIFRL
                     NDDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2518 a         2584 c         2430 g         2265 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgcgt ataaaaaaca ttcggggcta aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 gggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat gacggttgtc gcatactgcc atattgttgc ggataagccc
      481 ttttgcacgc gcgcccaagg gttctgtggc aataataaag gtgctgacag caatcgcctt
      541 gcgctccaaa ggcttgaata tcggattcaa accggcataa gtattgacgg cgtacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaaacgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcagcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gcgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accgcacgct
     3241 gccccggacg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa caacggagtc ggttggtttt ccggccagct gatatagacg gtttcgtccc
     3361 aagtcggcgg tgtaatgttg cgcacatacc agtaaggggc ggggacttgg cttttgacga
     3421 cgcgcccgtt gccgagcttg atatggtaaa tggcgaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagtgt agattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggattttgag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat gttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agttaaacgt ttgatgccgt ctgaaactct tgtaaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tctttggggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccaa tggtcaacgc catagaaaag ccgtgcaagg ccgcaccgcc
     5461 gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcaaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccgac ttgcgggccg ataaattcga cttggcgcaa
     5881 ggtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt accgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cggctttcgg cttccacacc gaaccaatgg aaatattctg
     6301 caatttgcgt ctgcgtccgt accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggcgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgcca aaaatgctgc cacccgcgct gtccaagctc agactgacgg caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtcgg caccggactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagagc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caagtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttcgg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ccgcgttttg
     7921 cagcgcggca tccactttga attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggcttttc
     8281 cgatgcggct tgtcggcaca aatcaatcga cttttgcaga aatcgcattg cgttccactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cactgttgcg cctttttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gtgccgggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgca cttgtccact
     8941 cgagcaaata tctgaaccgt ttggaatctt gccagcccca aaaaggcaag attttccgcc
     9001 tgaatgacga cactgcaatc agcacaggct cgctgtctgc cgcacgcttt gccgccggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgcaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggcgtcatg catgccattg ccgaataccg cctgaaacgc attgccgtca
     9241 tcgatttcga tgtccactac ggcgacggta cggcagaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcacattcc
     9421 gcgaagccgt ccgcaggcag tggctgcccc gacttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tagacgaatc gggcaggctc aacctgcacg
     9541 aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc tgccccggca
     9601 aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa tctgctgccg
     9661 agcatatccg cgtgctggca gggctgggca aatccgatgc cgcaaccgcc tatcagaaaa
     9721 cactggaccc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg cgccaaccta
     9781 cccaatccga ccgttac
//
LOCUS       DAWKQB010000066         9771 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.066, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000066 DAWKQB010000000
VERSION     DAWKQB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.066"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..201)
                     /locus_tag="WJL14_001222"
     CDS             complement(<1..201)
                     /locus_tag="WJL14_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7656290.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMP"
     gene            440..1339
                     /gene="crgA"
                     /locus_tag="WJL14_001223"
     CDS             440..1339
                     /gene="crgA"
                     /locus_tag="WJL14_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ7656291.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSESYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPSSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1563..4778)
                     /gene="carB"
                     /locus_tag="WJL14_001224"
     CDS             complement(1563..4778)
                     /gene="carB"
                     /locus_tag="WJL14_001224"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ7656292.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4785..5333)
                     /locus_tag="WJL14_001225"
     CDS             complement(4785..5333)
                     /locus_tag="WJL14_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656293.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5336..5899)
                     /locus_tag="WJL14_001226"
     CDS             complement(5336..5899)
                     /locus_tag="WJL14_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ7656294.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6750..7139)
                     /locus_tag="WJL14_001227"
     CDS             complement(6750..7139)
                     /locus_tag="WJL14_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ7656295.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLAEILRVLQELEKQAAR"
     gene            complement(7712..7837)
                     /locus_tag="WJL14_001228"
                     /pseudo
     CDS             complement(7712..7837)
                     /locus_tag="WJL14_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7883..9016)
                     /gene="carA"
                     /locus_tag="WJL14_001229"
     CDS             complement(7883..9016)
                     /gene="carA"
                     /locus_tag="WJL14_001229"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ7656296.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNQTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLNSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2222 a         2500 c         2496 g         2553 t
ORIGIN      
        1 cggcatctgc caaatcacag catccatcca aacgaatttt tcgatttctg cctcaacatc
       61 atagccggca tcaatcacgg tttcttgaac attgtgtccg agcgcggtca aaacttcttt
      121 cgcttttttg tgaagcgtgc ggtttaaccc gccgtgagaa tgtccgaacg ccttgccgcc
      181 gtctaataat aaaatattca tttgttacct cgtttgtgaa ttgatgaatg tattgtgccg
      241 ttttacttta taatttaaaa gtgcaaaaat aagaaaacac ttttgcttca aatgaaataa
      301 tcagatgttc caacacacgg gatggcacat aaagcaccgc cctgtgtgcc gtcctgattc
      361 ggaaggggtt acacccctcc caaataaagt ctgatcctgc cgccctaaag ggcggggttc
      421 caaccgaaaa ggaaacacga tgaaaaccaa ttcagaagaa ctgaccgtat ttgttcaagt
      481 ggtggaaagc ggcagcttca gccgtgcggc ggagcagttg gcgatggcaa attctgccgt
      541 aagccgcatc gtcaaacggc tggaggaaaa gttgggtgtg aacctgctca accgcaccac
      601 gcggcaactc agtctgacgg aagaaggcgc acaatatttc cgccgcgcgc agagaatcct
      661 gcaagaaatg gcagcggcgg aaaccgaaat gctggcagtg cacgaaatac ctcaaggcgt
      721 gttgcgcgtg gattccgcga tgccgatggt gctgcatctg ctggcgccgc tggcagcaaa
      781 attcaacgaa cgctatccgc atatccgact ttcgctcgtt tcttccgaaa gctatatcaa
      841 tctgattgaa cgcaaagtcg atattgcctt acgggccgga gaattggacg attccgggct
      901 gcgtgcacgc catctgtttg acagccgctt ccgcgtaatc gccagtcctg aatacctggc
      961 aaaacacggc acgccgcaat ctacagaaga gcttgccggc caccaatgtt taggcttcac
     1021 cgaacccagt tctctaaata catgggcggt tttagatgcg cagggaaatc cctataagat
     1081 ttcaccgcac tttaccgcca gcagcggtga aatcttacgc tcgttgtgcc tttcaggttg
     1141 cggtattgct tgcttatcag attttttggt tgacaacgac atcgctgaag gaaagttaat
     1201 tcccctgctc gccgaacaaa cctccgataa aacacacccc tttaatgctg tttattacag
     1261 cgataaagcc gtcaatctcc gcttacgcgt atttttggat tttttagtgg aggaactggg
     1321 aaacaatctc tgtggataat tctataaaaa cctgtcataa aattgttttt actcacttaa
     1381 taactttgag aaaaaataaa taagatgatg tatagccgcg gcaatggcag tctatatggg
     1441 gagcgtgata gggctgttcc ttatgtacgg cttgtgtgtg gcaacatcgg ctttccaaat
     1501 gacaaaatgc cgtctgaaac cgcaaaacgg tttcagacgg cattttcaac ctgattcagg
     1561 tatcagttgc ggtttttcaa acgcccgtgc aactcttgaa cgctgtacac gcccagatgg
     1621 tcgcggcttt tcgcgccttc gctcatcgct tcgccgccgg cagtggtggt gtattgcggc
     1681 acacgttgct gcaatgcgct ttggcggatg atgtggctgt cggacacgga ttgcggatcg
     1741 ctggaaacgg tattcacgac cagtgcgatt tcaccgtttt tcagcgcgtc gccgatgtgc
     1801 gggcggcctt cggggacttt attgatggtt tggacaatca gcccgtgttc ggtcaggtat
     1861 tgcgccgtgc cgcgtgtggc gcagatgccg tagcctaagg cttggaagtt tttggcggtt
     1921 ttaatgacgc gttctttgtc ttcttcgcgc acggagagga agattttgcc ggtcgggttg
     1981 aggcgttcgc ccgcgccgag ttgggctttg tagtaggctt cgccgaagct tgcgcccacg
     2041 cccatgactt cgccggtgga gcgcatttcc ggacccaaaa tcgtatccac gcccgggaat
     2101 ttgatgaagg ggaacacggc ttctttaacg gcatagaaat cggggacaac ttctttttcc
     2161 acgccttgtt ctttcaggga gatgcctgcc atgcagcgcg cgccgacttt ggcgagcggc
     2221 acgccggtgg ctttggagac gaagggcacg gtgcggctgg cgcgcgggtt cacttccaat
     2281 acgaacacta cgccgtcctg cacggcaaac tgcacgttca tcagtccgac cacgcccagc
     2341 gcgtacgcca tcgctttggt ttggcggcgg atttcgtctt ggatttcttc gcttaaggag
     2401 tagggcggca gcgagcagcc ggagtcgccg gagtggatgc ccgcctgttc gacgtgctgc
     2461 atgatgccgc cgataaccac atctttgccg tctgaaacgc agtccacatc gacttcaatc
     2521 gcgttgttca ggaagaagtc gagcaacacg gggctgtctt cggaaacctg cacggcttcg
     2581 cgcatatatt tttgcaactg ctcggcggag tggacaatct gcatcgcgcg tccgcccaaa
     2641 acataagacg ggcgcacgac cagcggataa ccgatttctt cggctttgac gagtgcttct
     2701 tcttcgttgt gggcgatgcg gttgggcggc tggcgcaggc ctaagtcgtt caacactttt
     2761 tggaagcgtt cgcggtcttc ggcggcatcg atgctgtcgg cggacgtgcc gatgatgttc
     2821 acgccgtttt caaccagcgc gttggcgagt ttgagcgggg tttgaccgcc gtaatgcaca
     2881 atcacgcccc acgggttttc ggtgcggacg atttccaaca cgtcttccaa tgtcagcggc
     2941 tcgaaataga ggcggtcgct ggtgtcgaag tcggtggaca cggtttctgg gttgcagttg
     3001 accataatcg tttcaaaacc ggattcgcgc agggcgagcg cggcgtgaac gcagcagtag
     3061 tcaaactcga tgccctgacc gatgcggttc gggccgccgc cgagaatcat cacttttttg
     3121 cggtcggaag gacgcgcttc gcattcctct tcgtaagtgg aatagaggta ggcggtttcg
     3181 gtggcgaact cggcggcgca ggtatcgacg cgtttgtaaa ccggatgcag cttcagcgcg
     3241 tagcggtgtt cgcgaacttc tttttcgctt acgttcaaca attgtgccaa acgtttgtcg
     3301 gagaagcctt tgcgtttcag acggcgtagg gcggcgaaat ccaaatcact caaaatgccg
     3361 tctgaaaccg ctttttcttc cttcatcaag tcttcgattt gcgccaggaa ccaagggtcg
     3421 atggcgcaga tttcgtggat ttcttccagc gtgaagcccg cgcggaacgc gtctgccaca
     3481 aacagcatac gttcggggcc ggggtttgcc agttcgcggc ggatttccgc tttgtcggag
     3541 cttctcggat tgaagccgca caagcctgtt tccaagccgc gcagggcttt ttggaagctt
     3601 tcctgaatcg tgcggcccat cgccatcact tcgcccacgg atttcatctg cgtggtcagg
     3661 cggtcgtctg cggcagggaa tttttcaaac gcaaaacgcg ggattttggt taccacatag
     3721 tcgatggacg gctcgaatga cgcgggggtt ttgccgccgg tgatgtcgtt gcgcaactcg
     3781 tccagcgtaa agccgaccgc cagtttcgcc gccaccttcg caatcgggaa gcccgtcgct
     3841 ttggaagcca gcgcggacga acggctcacg cgcgggttca tctcaatcac aatcatctcg
     3901 ccgttttcag ggtttactgc aaactgaacg tttgagccgc cggtatccac gccgatttca
     3961 cgcaacactg ccaacgaagc gttgcgcatg atttggtatt ccttgtccgt cagcgtttgc
     4021 gccggcgcaa ccgtaatcga gtcgcctgta tgcacgccca tcgggtcgaa gttttcaatc
     4081 gagcagatga tgatgcagtt gtcgttctta tcgcgcacca cttccatctc gtactctttc
     4141 cagccgagaa cggactgctc aatcaacagc tcgtgcgtgg gcgacgcatc gaaaccgcgt
     4201 tcgcaaatcg ccaaaaactc gtctttattg taggcaatgc cgccgcccga accgcccatg
     4261 gtgaaagaag gacgaatcag ggtagggaag ccgacctgtt cttgcgccgc caaagcttcg
     4321 ttcatcgtgt ggcagacaaa agatttcggg caagacaaac cgattttttc catcgcttct
     4381 ttaaagcggc cgcggtcttc cgccttgtca atcgcgtctt ccgtcgcgcc aatcagctcg
     4441 acattgtatt ttgccagcac gccgttgcgt gccaaatcca gcgcacagtt cagcgcggtc
     4501 tgaccgccca tcgtgggcag aatcgcatca ggccgctcct tggcgataat cttctccacc
     4561 gtctgccaca taatcggctc gatgtaggta acatccgcca tttcagggtc ggtcataatc
     4621 gtggcggggt tggaattcac caaaatgact ttatagcctt cttcacgcaa agccttgcag
     4681 gcctgtgcgc ccgaatagtc aaattcgcag gcctgaccga taacgatagg gccggcgccg
     4741 atgataagga tggattttag gtcggtacgt ttgggcatgg gtggttactc ttgaattaaa
     4801 gaaggttgtt ttgttacata gcttttaaga aaagtatttc ccttttctaa aattgaagct
     4861 aaaacatcat cttctatgtt agataaaata ttctctaaat ctgcgcttga tttaatttta
     4921 aagaattctt ttgtttgaga atggtataca agagaaggtt tatttagctg taaaagttct
     4981 gctatattat ttaatgaacc aatactctta ccatcccata aaataaagcc ataatcagca
     5041 atttctgcca ttttcttatc ttttgctgta taaaataccc ttccagttcc tttgctgtct
     5101 acttggacaa actgccaatt ccccacatta tttcggtaaa ttttgccaga aaagtaaata
     5161 tgcacattag cataatcttg ttcttgtaga aatttttgga tagctttatc agccccatta
     5221 gcatctccaa taacaatatc aaaattattg ctaagaatat tgttaattct ttctcgtatt
     5281 tgtggattta gtcgtgaaat gctgcgtgaa ccagaaaaga aaatagtgcg catttttatc
     5341 ctctagtctt tgtcattgct aaaacaatta cagatctagc attttttgaa aacaattttt
     5401 cagttgatat ttccaatgtt gctccgctat caaataaatc atcaattact aaaatattct
     5461 tattggatag atctatattg tcaattgtga tggaattatt cagtatttct agtttttctg
     5521 atttttcttc aatatttttg agtggtgtgt gtgagctaga ttttcttaaa ataggagaat
     5581 atggaatatt caagcgatca cttagttctt tggcaattaa ttgaactggt tgattaatac
     5641 gctctgttgt aaaaggggcg ggaacaatta aatttataga ttctaaacca ctaaattttt
     5701 gtaaaatata gtctaccagc aaagaaacat tttgggtttg attacgatat tttaattgat
     5761 aaacccattc tccaatcaca cttctcttgc tatcaaagtg aggatgccct aattcatcat
     5821 aacctaacaa aatacttttt tgcatatgat ggtctaaagc aaatcctttt gtccagttac
     5881 catttagttc tatcgccatt tcattgactc cttgtcaaaa ttaagtataa cgagcaatcc
     5941 tagcaagccg tagcccgtat gtagggtatg tgcggtacgt atgcacgcgt tctccatttt
     6001 ccctgcaacc ccaacccacc gcgtgcgtgc cttgcggcac actctctgcc gatgagttca
     6061 aaggtcgtct gaaaaaacgt tcgcggcgtt tgttccacct gccttgcgat aaaggctgcc
     6121 tgaaaaattc ccgctgttga tatttcgggc attctttctc aaaccgcctg caaccgcaaa
     6181 atccgctgca acctgccccc tcccccgtgg gggagggttg gggagagggc attccccgaa
     6241 tttcggcaac ctttcccaac aactcaacca ccgaaataca agccttgtgg cttgttgccc
     6301 tctctctagc tctctcccac gggagagagg acggagtggc tgtagggcta agggttctgc
     6361 aaggaaacag gttgtgcagg ttgctttttg cttttcagac gacctgaaat caacggtaaa
     6421 tccaagtcgc tttcaaaccg cctgcaaccg caaaatctgc tgaaacctac tccctccccc
     6481 gtgggggagg gctggggaga gggcattctc cgaatttcgg caacctttcc taacactcta
     6541 accgccaaga cacaagcctt gcggcttgtt gccctctctc tagctctctc ccacgggaga
     6601 gaggatgggg tggctgttgg ggctaaggtt tctgtaaact aatcaggtta tgcaggctgc
     6661 tttttgtttt tcagacggcc tttataaatg ctcaattcag tcggctttat cctattattt
     6721 tcaataatcc aatcaaaatc agccatctgc tatcgtgcag cttgcttttc caattcctgc
     6781 aatacgcgca ggatttccgc cagtacatcg tttgtctgct gcaaaatttc gtgattccaa
     6841 aaacgcagca cggtaaagcc caggctgttg agatatgccg tccgcgcgtg gtcgtatgcg
     6901 gcttgttctg tgtgctgccc tccgtctgct tcgacaatca acttgggcgt tacgcacata
     6961 aaatcaacaa tatagtttcc catcggctgc tggcggcgga atttatagcc gttcagacga
     7021 cctgcccgca ggtgctgcca caattttgct tccgccccgc tcatttcttg gcgcatggct
     7081 ttggcgcgtt ggcgcaagat ggggttgtcg gtggtcaaga gtttctcgtg cgggttcatt
     7141 ttgttgtcgt gctgtttgct gccctctctc taactctctc ccacggggag agaggacggg
     7201 gcggttgttg gggttaaagg ggctgtaagg aaaacaagtc gttcaggcca ctttttactt
     7261 ttcagacgac ctgcaatcaa cggttgtaaa tccaagttgc ttttcaaacc gcctgtaacc
     7321 gcaaaacttg ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcattct
     7381 tcgaatttcg gcaaccttcc ccaacaccct agccgcccaa acacaagcct tgctgtttgt
     7441 tgccctctct ctaacgcttt cccacaggga gacaggacgg ggcggctgta gtgattaagg
     7501 gttttgcaag gaaaacaagg aaaacaagtt gtacaggctg ttttttattt ttcaagcgac
     7561 ctgaaatcaa cgcttgtaaa tccaagtcgc ttttcaaact gcctgcaacc gcaaaatctg
     7621 ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcatttt ccaagttgcg
     7681 gcaatctttc ccaaatccct tagtcttcta aaatacaagc cttacggttg ttgccaaatg
     7741 aaacttcttc catacccaaa acttgcgtgt aaaacgcaat ggttcggtca atgtcggcaa
     7801 cggtcagtac cagatggtcg agtgcgctaa tcttcatttc ccgctttctt tttcaggcgg
     7861 cggcagtgcc gtctgaaagc cattatgccc gttttgccgc tttcatattg ccaatgaatt
     7921 tgtcaaacaa atagccgaca tcttgcggac cggggctggc ttcggggtgg ccttggaagc
     7981 agaacacagg tttgtcggtc agctcgatgc cttgcaaagt gttgtcaaac aaggatttgt
     8041 gggtaatgcg tgcgttagcg ggcagggtgt cggcatcaac ggcaaaaccg tggttttggc
     8101 tggtgatgac gactttgccg ctgttcaaat cttgcacagg gtggttcgca ccgtggtggc
     8161 tgaagcgcat tttcagggtt ttcgcgccga tagcgaggct gatgagctgg tgtcccaagc
     8221 agatgccgaa aatcggtttg ccgctttcca tcagtttttg cacggcttcg atggcgtagg
     8281 tgcaaggctc ggggtcgccg gggccgttgg acaggaacac gccgtcggga ttgagtgcca
     8341 acacgtcttg tgcgctcgtt tgggcgggga cgacggtcag gcggcagccg cgcgaggcga
     8401 gcatacgcag gatgttggtt ttcacgccga aatcgtaggc gacgacgtgg taaggctgtt
     8461 cgtcaggggt aacgaaacct ttgcccaatt cccattcgcc ttccgtccat tcgtaagttt
     8521 ctgtgcagga aacttctttt gccaaatctt tgccgaccat actgccgaac gcggcaatca
     8581 gttcttgcgc tttttcaacc gtagcgtccg cgccggtcag aatcgcgccg ccttgcgcgc
     8641 ctttttcgcg caacagcgtg gtcaggcggc gggtatcgat gtcggcgatg gcgacggttt
     8701 ggttgcgtac caaatagtcg tgcaggcttt cggaggcgcg gaagttgctg tgcaagagcg
     8761 gcaggtcgcg gataatcaaa ccggcggcgt aaacgctgcg gctttcttca tcttcggcgt
     8821 tggtaccggt attgccgatg tgtgggtagg tgagggtaac gatttgtttg cagtaggacg
     8881 ggtcggtcag gatttcctga tagccagtca tcgaagtatt gaacacgact tcgccggaag
     8941 tcgaaccttc gtaaccgatt gatgtgccgt ggaatacgct gccgtcagcg aggacgagga
     9001 gggcgggggt gctcatgatg ggaatcctgt ttttaataaa attcttgaca atgccgtctg
     9061 aagggacttc ggcagggggc atccggtcgg aaaaatccgg tcaaaaaaaa acacgccgcg
     9121 caagatagac gcgtaacgtg cttttcgggc aagtgcctat accctgaaag cagcgtattt
     9181 taagacgaaa catacccgat ttcaagcgca aacgcggcag gcgggctgat atgttcgcgc
     9241 gaaaacggaa atccgtggcg taaaaccgcc cgctccgcgc ccactatgcc cgttacgcat
     9301 ttccccacgt ccgcccggcg aaactatggg aatacccgaa tcgtcaggaa tacctgaatc
     9361 gtcaggaata cctgaatcgt caggaatacc cgaaccgtca ttcccgcgca ggcgggaatc
     9421 cagacctgtc ggcacagaaa cttatcgggt acaacggttt cttgagattt tacgtcctag
     9481 attcccgcct gcgcgggaat gacgcgtttc atttgccgcc ccctcccgaa aaacgcaaaa
     9541 aatgccgtct gaaagctctg tagggagggc ttcagcccac cgattccatc aatcccacat
     9601 ttccgccgtt ttcaccgaat ccgccccttt tcacaatcca gtcctacgca actgaaccgt
     9661 cattcccgcg caggcgggaa tccggacttg tcggcacgga aacttatcgg gtacaacggt
     9721 ttcttgagat tttacgtcct agattcccgc ctgcgcggga atgacggcgg a
//
LOCUS       DAWKQB010000067         9766 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.067, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000067 DAWKQB010000000
VERSION     DAWKQB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.067"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(312..545)
                     /locus_tag="WJL14_001230"
     CDS             complement(312..545)
                     /locus_tag="WJL14_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ7656297.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WJL14_001231"
     CDS             complement(711..911)
                     /locus_tag="WJL14_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ7656298.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJL14_001232"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WJL14_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ7656299.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVGRYYTEARPALLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WJL14_001233"
     CDS             complement(2010..2450)
                     /locus_tag="WJL14_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ7656300.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WJL14_001234"
     CDS             2771..3709
                     /locus_tag="WJL14_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ7656301.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="WJL14_001235"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="WJL14_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ7656302.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4216..4782
                     /locus_tag="WJL14_001236"
     CDS             4216..4782
                     /locus_tag="WJL14_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7656303.1"
                     /translation="MLLKNILPFAHCLLRQALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRLILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSAALSLLKENGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYSFTNRKNSPPYLLAFEKLPQK"
     gene            4836..5210
                     /locus_tag="WJL14_001237"
     CDS             4836..5210
                     /locus_tag="WJL14_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ7656304.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCLACIVYLAKTKVLPF"
     gene            5220..5714
                     /gene="folK"
                     /locus_tag="WJL14_001238"
     CDS             5220..5714
                     /gene="folK"
                     /locus_tag="WJL14_001238"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ7656305.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPRAHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(5841..6320)
                     /locus_tag="WJL14_001239"
     CDS             complement(5841..6320)
                     /locus_tag="WJL14_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ7656306.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRSLRLSWVGSARELDRKDSAYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJL14_001240"
     CDS             complement(6540..7277)
                     /gene="ubiE"
                     /locus_tag="WJL14_001240"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ7656307.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPAMGRLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7320..7688)
                     /locus_tag="WJL14_001241"
     CDS             complement(7320..7688)
                     /locus_tag="WJL14_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ7656308.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7808..9427
                     /gene="mnmC"
                     /locus_tag="WJL14_001242"
     CDS             7808..9427
                     /gene="mnmC"
                     /locus_tag="WJL14_001242"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ7656309.1"
                     /translation="MDNLAWNGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPNFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPTFVRALLSHPLIGLHEDTPLTDISHDGEKWIASTPNGTFTATHIIYCTGANSPY
                     LPETNLATLPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRAIIRAIVRRKDLTP"
BASE COUNT         2602 a         2980 c         1973 g         2211 t
ORIGIN      
        1 ctaccgatta cacaaacaca tatcatcttt acacaatcat gcttccatca acagtaaaac
       61 atgatatgat tgccaacaat aacatctcac aataaatttt ctaattttta ttgaaaaaat
      121 caataaataa gaattctccc aaccgacaaa taaaataaag aaagggttaa tatgcaacat
      181 cgaagaagac tagcaattta ccaagcatcc aaacgtgctt cctttaccgg caggtcatcc
      241 gccccgcaaa aacgtaaaga acgttaattt gaaaaaaatg ccgtctgaag tcctgcttca
      301 gacggcattt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag ttctgccaaa
      661 aacagtaaaa atgccgtctg aacagttcag acggcatttc gaaaaccgtt ttacgcttga
      721 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccgc agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat tgtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc gaagcagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacatt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaatccgt gctgcaccaa gtgcgtggcg aaggcatggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgcctgact tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atacctgttt ttttctgact gacaaacaag gcatcgcaac tcctgccctt caacaaggcc
     1381 ggacgcgcct ccgtgtaata gcgtcccacc caatacgccg attcctgccc catcgggacc
     1441 atcctctgct tgtcgccctt gcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat taattcaagc
     1561 agggccttgt cccgcaaacc gtgcggcgtg tcggtatccg gtgcggcaag cagtcgggaa
     1621 atctgctgtt ctgtaatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgat tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agacgcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caactgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacgataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ctgacgaaat cgaccgcatc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgcaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttctaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaac caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcatc tgcccgattt cacaatcata atagcggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcatgtt
     3901 ccggtttcga tttacttagt taacggtatc aaattgcaag gtcaggttga gtctttcgat
     3961 caatacgttg ttctcctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccgacttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accggacaac ggtaaggtat ttttattttc
     4201 agacggcatt tgaaaatgtt attgaaaaac atccttccat tcgcccattg ccttttgcgg
     4261 caggcacttc ccgaaggcgg caatgcgctg gacggcaccg ccggcaacgg acacgacacc
     4321 cttttcctcg cacaaaccgc aggcatccgg gggaaagtgt gggcattcga catccagccg
     4381 caagccctga acaacacccg atgccgtctg caggaagcag gttacagcaa tgtacggctc
     4441 atcttggacg gacatgaaaa cctgaagcaa tatattccaa agccgctgga tgcagccatt
     4501 ttcaatttcg gctggctgcc cggcggggac aaaagcctta ccacccgcac ggaaaccagc
     4561 attgccgccc tttccgccgc cctatcccta ctgaaagaaa acggtatgct tattgccgtc
     4621 ctctaccccg ggcacgaaaa cggcaaacag gaggcagaag caatcgaaca atgggcaaaa
     4681 aacctgcctc aagaacagtt tgccgttctg cgttacagct ttaccaaccg gaaaaacagc
     4741 ccaccctatc ttttggcatt tgaaaaactg cctcaaaaat aactgtttgc ggtaaaataa
     4801 gccgattcta acccttattt tgatacgagc cgaccatgca gtatctgatt gtcaaataca
     4861 gccatcaaat cttcgttacc atcaccattt tggtattcaa catccgtgtt ttcctacttt
     4921 ggaaaaatcc agaaaagccc ttggcgggct tttggaaggc actgccccac cttaacgaca
     4981 ccatgctgct gtttacggga ttgtggctga tgaaaattac ccatttctcc ccgttcaacg
     5041 cgccttggct cggtacaaaa atcctgcttc tgctcgccta tatcgcattg ggtatgatga
     5101 tgatgcgcgc ccgtccgcgt tcgaccaagt tctacaccgt ttacctgctc gccatgtgtt
     5161 gcctcgcctg catcgtttac cttgccaaaa ccaaagtcct gcctttctga aacaccgtta
     5221 tgaacaacag acattttgcc gtcatcgccc tgggcagtaa tcttgaaaac cctgcccaac
     5281 aggtacgcgc cgcattggac acgctgtcgt cccatcctga catccgtctt aaacaggctt
     5341 cctcactgta tatgaccgcg cccgtcggtt acgacaatca gcccgatttc gtcaatgccg
     5401 tctgcaccgt ttccaccacc ttggacggca ttgccctgct tgccgaactc aaccgtatcg
     5461 aagccgattt cggacgcgaa cgcagcttcc gcaacgcgcc gcgcacattg gatttggaca
     5521 ttatcgactt tgacggcatc tccagcgacg acccccgact caccctgccg catccgcgcg
     5581 cgcacgaacg cagtttcgtc atacgccctt tggcagaaat cctccctgat tttattttgg
     5641 gaaaacacgg aaaggttgcc gaattgtcaa aacggctggg caatcaaggc atccgtcttt
     5701 taccggataa gtaattccgc acgcggatgc cgtctgaatg ccggtttcag acggcatttt
     5761 tcctttgccg ccaacacgcg tgcaaaaaaa tcgccccttg ggaaaggggc gcaaaaggaa
     5821 cacaaaccac taccaaaact ttaaatctgc aacattgcct gccgcatact gtatccgaca
     5881 ggatataaag ccctcactaa atcgtttcga gaaatccaaa cttcttcatc gccgacagaa
     5941 aatctgcctt tctccggcac cagctccaac aaaaacggtt gaaccgccgt atgcagcctg
     6001 tccttacacc gccccagctt tctggacaag gcggacaggg cgcgtttgta ggcactatcc
     6061 ttgcggtcaa gctcccgcgc actgccaacc cagctcaaac gcaggctgcg gctttcatcc
     6121 tcaatcagca aaatgcctgt tttgacttcc cccacctcgg gctcgcatga aagcgcaata
     6181 taatatttgc cgtcgatgtt gacatacgcc gcttcatgca cctgacagtt gcagccggaa
     6241 gtggcaagca gcgaataaac agagtcccga tgcagtttca aaatatcaag ggaaatctgt
     6301 ttgaaaatac tttccggcat ttttccctcc tttcgtctgc tgcgttgttt actggattga
     6361 ctgcattata cagacatctc ccaatcttgc aaatgataat tgttcctatt ttttaaaaaa
     6421 tttataaatt attgatggat atgtgaatta attttaagat ttaaaatctc tttctcaaaa
     6481 aaacacaaaa atgccgtctg aaacgtgttc agacggcatt ggctgcacag ccagtccgtt
     6541 taaaatttca cgcccttatg cagcgcgacg atgcccgcac tcatattgtg ataatccacg
     6601 ctgtcgaagc ccgcatccag catcatctgt ttcaaagttt cctgatcggg gtgcatacgg
     6661 atggattcgg caagatactg gtaactctcc gcatctttcg caatcagcct gcccatcgcc
     6721 ggcagcagct tgaacgaata gaaatcatac gcgccttcga gtggcttgta gattttggaa
     6781 aactccaata ccagcaatgt cccgccgggt ttcaaaacac ggtacatctc tttcagcgcg
     6841 gcatctttat gcgtcatatt ccgcaagccg aacgccacgg aaaccaagtt gaaataattg
     6901 tcagggaaag gcagtttttc cgcatcggca agcgacacgg gcaaaatcat gccttcgttc
     6961 aaaagacggt cgcgcccgac ggtcagcata gaagaattaa tatcggtcag ccaaacctcg
     7021 ccttccttgc cgacgcgttt cgcccaccct cgggacaaat cgcccgtacc gcccgcaatg
     7081 tccaacactt tatcgccttt tttcaggtgc gccgtgttga tggtgaaatg cttccacacc
     7141 ctgtgcagcc ctgccgacat cacatcgttc ataatgtcgt agtttttggc gacggagtgg
     7201 aacacttccg ccacttttcc ggctttctca tcttcgttga ccgtgctgaa cccgaaatgc
     7261 gttttctgtc cgcccatatt ctaaccacct ccctgatgcc gccctacggc atcccaaact
     7321 taacaatctg attgcttttc aaaacgggaa gcacccgccg ccgccaattt gtccaaatat
     7381 tcctgccaca tcgcatcgta tccgtatgcc agtctgtgca gatacgccca atcgtaaaga
     7441 ccgctgtcgt gcccgtccga aaaactgatt ttcagcgcat actgtccgac aggctgcaaa
     7501 tccgtgattt ggacatccgc cttgccggtc tgcaaaacat cctgtcccac gccgtgtccg
     7561 cgcacttccg cactcggcga atagacgcgt aaaaattcgg caggcagatt tttaggttcg
     7621 tccccataag tcaaaaccaa cacggtacgg tttttcaaca aacggatttc aacaggaata
     7681 ttattcaaaa aacacctcgg ttcggcatac aatatccggc aggcgcaggc gtgccttcaa
     7741 acggcgcgta ttttaacagc tttatcccga taccgatatt caaacccaca tttcagacgg
     7801 cattgatatg gacaatctcg catggaacgg cattcccgac atccgcacac tcgaccaaac
     7861 catccgcaaa cacgcacacc cgctcaacct gattgtctgc ctccccgata atcagattcc
     7921 caattttcaa accgcacaag atgcttcgga cgcggaatgc cgtctgaagc accgtttgga
     7981 tcaggcaacc cagtgcctcc agttcgacag catcaacctg attgaacaca tcctgcccga
     8041 tgtccgcttc tggctggttc ccccttcacg cacccgccgc ctgcacgaac acttccacca
     8101 catttcctgg cagaccgaag ccatcccgca aaccgaaagt aagcccgaca aaccctggtt
     8161 tgcacttcca caaacatccg aacggcaaaa accggaacac atcctcgtta tcggagcggg
     8221 catatccggc gcggcaaccg cccacgcctt agcatcatac ggcatttccg ttaccgtatt
     8281 ggaagcccga aaagccgccc aagccgccag cggcaaccgc caagggctgc tctacgccaa
     8341 aatctcgccg cacgacaccg aacaaaccga actgctgctt gccggctacg gctacaccaa
     8401 acgcctgctc ggacatatcc tgcccgaatc cgaaacctgg ggcggcaacg gcatcatcca
     8461 cctcaattac agccgcaccg aacaacaacg caatcacgaa ttgggtttgc aaaaacacca
     8521 taaccacctc taccgcagca tcacgcaggc agaagccgaa aaaatcgccg gcatccctct
     8581 gaacacgccc tacgccgaac cattatgcgg actgttttgg cagtacggcg tatggctcaa
     8641 tcctcccaca ttcgtccgcg ccctcctcag ccatccgctc attggactac acgaagacac
     8701 accgttaacc gacatttccc acgacgggga aaagtggatt gcaagcacgc caaacggcac
     8761 atttaccgcc acacacatca tctactgcac cggtgcgaac agcccctacc tacccgaaac
     8821 caacctcgcc accctgcccc tcaggcaaat acgcggacaa accggcctca caccgtccac
     8881 cccgttttcc gaacaactgc gttgcgccgt ttcaggcgaa agctacatca gcccgtcgtg
     8941 gcacggactg cactgctacg gcgcgagttt tattcccaac agcagccata ccggatggaa
     9001 cgaagccgaa gaagcctcaa accgccaagc attggcacac cttaaccccg ccctttccga
     9061 atcattgttt gccgccaacc caaaccccca aaaacaccaa gggcacgccg ccatacgctg
     9121 cgacagcccc gaccaccttc ccctagtcgg cgcactcggc gacattgccg ctatgcaaca
     9181 aacttacgcc aaactcgcgc tggacaaaaa ctatcgcatc gatgccccct gcccgtacct
     9241 gcccaatgcc tacgccaaca ccgcccacgg cacacgcggg cttgccaccg cccccatctg
     9301 cgccgccgcc gttgcagccg aaatcctagg cttgccccat cccctctcaa aacgcctgcg
     9361 ccacgcccta caccccaacc gcgccatcat ccgcgccatc gtcagaagga aggatctaac
     9421 cccttaagcc tttgccgcat gccgtcccga ccacagaaaa tcatattcaa ataaacatac
     9481 caatcctgac ggtaaaatat cgaaaacacc cataacattt tcttcccaaa ataaggagta
     9541 aggaatgcct aacaaattgc ctttgctgtc caagatgaat gttccgtaac attcgaagac
     9601 ttccttaacc ctgccagccg tttcggtttc tgtacctcct gttagataag gaaggaatga
     9661 aaaagccctg ttgattgtat gaagtaaagg gcgttaattt ttgttaatct tcccttctca
     9721 ggaactcaat atataaggat gttaattttt atttcccttc tcgagg
//
LOCUS       DAWKQB010000068         9656 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.068, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000068 DAWKQB010000000
VERSION     DAWKQB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.068"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL14_001243"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WJL14_001243"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ7656310.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WJL14_001244"
     CDS             complement(1457..1846)
                     /locus_tag="WJL14_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ7656311.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFEHYDKNKLKQNKYFNVTLSKVSPDHFTLKATPNPTTND
                     GETCVVTLNDGGIIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WJL14_001245"
     CDS             2072..3250
                     /locus_tag="WJL14_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ7656312.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQASYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKENATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WJL14_001246"
     CDS             3316..5250
                     /locus_tag="WJL14_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ7656313.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHKAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5352..6134)
                     /locus_tag="WJL14_001247"
     CDS             complement(5352..6134)
                     /locus_tag="WJL14_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ7656314.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSKTPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6273..8462
                     /locus_tag="WJL14_001248"
     CDS             6273..8462
                     /locus_tag="WJL14_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ7656315.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVQTAFVEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     VKLIENWAEQGWIETTEAAKPVLRSCHGQASHSEFVLNADQRQASDAIQTALGRFRPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPLPDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPVPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRCIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLASLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8508..9581)
                     /locus_tag="WJL14_001249"
     CDS             complement(8508..9581)
                     /locus_tag="WJL14_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7656316.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSVRVSILL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITVPAILTAA
                     VFVLYLLTFVPIFRANAFTDDPE"
BASE COUNT         2255 a         2525 c         2821 g         2055 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaataccg tctgaacagc gttcagacgg catttcgatg tgtactgcca
      121 cgtcaaatca gtggtgatgg ccgcagccgc attctttttt catatcgatt accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaaccttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagcccga tggcaaccac acgtccgcct gcgcggacgc
      421 aattcacggc agagttgaat gcggcagcag atacggcggt tacgaccgca gcgtgtgcgc
      481 cgccggtttt ttcctgaatc actttggcag cgtcttcttt ggcggcgttg acaaccaaat
      541 ccgcgccggt ttctttggca aacgccagtt tgtcgtcgtt gatgtcgatg gcgacaacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtca
      721 cgccggcaca agtaatgctg gaagcttgcg caggatccaa accttcaggg actttgaccg
      781 cgtaatcggc actcacgatg cagtaggtcg ccataccgcc gtcggcggtg tagcccgcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca gcttggaaag
     1321 gcgcggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaagatac cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tgataccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 gcgttgcctt aagggtaaaa tggtcggggc tgactttgct taaggtaacg ttgaaatatt
     1621 tgttttgttt cagtttgttt ttatcgtagt gttcaaacgt tcctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga taagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcgacgg cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggcttcttat attacggaag cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgca caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aaattggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggaaaacg cgacttccaa agaggatttg gaaagcgcgc aggatgcgtt
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggcttta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ctacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcagcagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggctgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacgaca tcgaaactgc caaaatgcag
     3541 cctgacgagc tggcggcatt gcgccgcgaa cgcttcggtt tcatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggacaac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgtgcggag aagttgttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg tgcgctcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccacaagc tgcacaaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gcatcgcgtc ggtggtttcc gtcgtcgcat tgggcaacgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acttaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tccgccatgt ccgtcatcgg cgcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaacaaggc ggccaaactc
     5221 aatccgatag acgcattggc gcaggattga ggttggcaca aagatgccgt ctgaagctgc
     5281 cgcgacaggc ttcagacggc atttgaaaaa ggcttggaac acggtttcaa gccttttgtt
     5341 ttgcagatgg attattggtt tttgcggatg atttcctcca gttggggcat cgggctgtag
     5401 ccgctttggc tgcgcccgtt ggggaagacg agggtcggcg tgccgttgaa gccgaattgt
     5461 tcgcccaagg aagtggtttc cgcgacggga ttgtcgcaga tgctgccgcc gaccgggaat
     5521 ttgcctttac gcatccaatc cgtccacgct ttggcgcggt cgggctgaca ccataagatt
     5581 tgcgccttgc gcgcggcatc ggggtgcagg ccggcaatgg gcatcataaa gctgtaaacc
     5641 gtcacgtcgg tcattttttc aaactcgtgt tccaagcgtt tgcagaacgg acaatcgggg
     5701 tcggagaaga cggcgacttt cagcttgccg ttgccgcgca cttctttgat ggctttgtcc
     5761 aaaggcaggg aggcgaagtc gattttgttc aaatcggcgg cgcgttcttc ggtcaggttt
     5821 ttgcgcgtgt cgatgttgat gagttcgccg acgaacatat agccgccttc ggcatcggtg
     5881 tagataatct gcctgccgct gacgacgact tcgtaaatgc ctttgaccgg tgttttgctg
     5941 acgctcaaca ctttcaaatc ttgggcggaa taggtttttt ccaaacgcgc tttcaaagag
     6001 gcggcaacgg atttgccggc ggactcggct ttgacggcgg gttcggcgtt ggcattggaa
     6061 acgggcgttt gcccgcaagc cagcagcggg aggacggtaa agggggtcaa gattttgatt
     6121 aacttggttt tcatataaag atgattgcgc gtgttggaaa agcggaattg tatcaaatct
     6181 ctgttgcgcc tgcattgcgc ctaggctcaa tttatcgtct gaaaatagct tccgcctgtt
     6241 aaaatacgca aaaaaatgat ttgcctgcct gtatgatcta ccaccgcatc gccgtaaacg
     6301 tgccgctttc agacggcctt ttgacttatt cccattccga tccgcttcct ccgggaacgc
     6361 gggtgcttgt gcctttccgc aataaaaccg tggtcgggat ggtgtgggaa acggatattg
     6421 cgcccgatat ggatacggcg cggattttga gtgttcagac ggcctttgta gaagaaaagc
     6481 cgttacctca aagctggcgt gatttgttgg cgtttacgtc gcgttattac cactatccga
     6541 ctgggcaggc ggtgtttgcc gcgctgccgc agggtttgaa ggaaacgcgc gcggtggaaa
     6601 tgccgcagcc gccgttgttt tacgcgctga acgaagcagg cagggcgcaa acgccgccgc
     6661 cggcgcggtt caacaaaaaa gcggctttgt gggacgcgct gctgtcgggc gaaatgacga
     6721 tggcagcgtt gaagcaggta aacgcgcagg cggtgaaatt aatcgaaaat tgggcggaac
     6781 agggttggat tgaaacgacg gaagcggcga aacccgtttt aaggtcgtgc cacgggcagg
     6841 cttcgcactc tgaatttgtg ttgaatgccg accaacggca ggcttccgat gccattcaga
     6901 cggcattggg acgcttccgg ccgtttctgc tgtacggcat caccggcagc ggcaagaccg
     6961 aggtgtattt cgatgcgatg gcgaaagtgt tggcgcaggg gcggcaggtg ttgtttctgt
     7021 tgcccgaaat caacctcacg ccgcagcttt tgaagcgggt ggaaaaccgt tttgccgacg
     7081 tgccgaccgc cgtgttgcac agccggatgg cggcaggcag gcgcacgcag gattatctgc
     7141 gcgcgatgtt ggggcaggcg aagctggtca tcggcacgcg gctggcggtg ttcacgcctt
     7201 tgcctgatgt cgggctgatt gtggtcgatg aggaacacga cggctcgttc aaacaggaca
     7261 acgaattgcg ctaccacgcc cgcgatttag cggtgtggcg ggcgaagcag agcggctgcc
     7321 ccgttgtgtt gggcagcgcg accccgagtt tggaaagctg gcacaaggcg caaagcggcg
     7381 cgtaccgcct gctgcaactg accgaacgcg cccataccgc cgcgcaactg ccgcaagtgg
     7441 acatcctcaa cgtaggccgt ctgaaactcg ataacggctt ctcgccgcaa gccttgcagc
     7501 ttttgaaaca gaactttgaa gcgggcggta tgtcgttggt gtacctcaac cgtcgcggct
     7561 tcgcgcccgc gctgttttgc ggcgactgcg gccatacctt cggctgcccg aactgctccg
     7621 ccaaaatggt gctgcaccaa cgcgcccgcc aactgcgctg ccaccactgc gaccaccgcg
     7681 aacccgtccc gttcaaatgc cccgactgcg gcaaccaaga tctgaccgcc gtcggacacg
     7741 gcacgcagcg cgtcgaagaa accctgcgcg ccttcctgcc caaggcagcc gtcgtccgtg
     7801 ttgacaggga cagcaccgcg cacaaaaacg actgggcgga tttgtaccgc tgcattgccg
     7861 acaacgaaat cgacattctg gtcggcacgc agatgctcgc caaagggcat gatttcgcgc
     7921 ggctcaacct cgttatcgtg ttgaacgccg acggcagcct gtacagcgcg gactttcgcg
     7981 cgccggaaag gctgtttgcc gagctgatgc aagtgtcagg cagggcgggg cgcgccgaca
     8041 aacccggcaa ggtgttgata cagacccaac tgcccgaaca tcctgtcttc gccgccgtca
     8101 aagcgcaaga ctatgccgtg tttgccgaaa acgaattgaa cgagcggcaa atgttcgcca
     8161 tgccgccctt cggtttccag accgccgtcc gcgccgatgc gccgcgcgtt gccgatgcga
     8221 tggaatttct caatgccgcc aaagaaaccc tcgcctcgct gttgcccgaa agcgtttccc
     8281 agttcggcgc ggccccgatg ctgatggtgc gcctcgccga acgcgaacgc gcgcaaatct
     8341 tcctcgaatc gacatcccga caggatttgc accgcgccgt gagtttgtgg gtgcaggtgt
     8401 tgcagcaaaa ccgcgacggt aaaatcagat ggtcggtgga tgtcgatccg caggaggctt
     8461 gattattggc aatccgatgc cgtctgaaac cgtttcagac ggcattttta ttctggatcg
     8521 tctgtaaacg cgttcgcccg aaaaataggt acgaatgtca gcaggtaaag cacgaacacg
     8581 gcggcggtca gaatcgcggg aacggtgatg aagaatatcg ggttcacgtt cattaaaaca
     8641 gcgcgcgaga cggcggcggc gaagaggatg gggacggcga tgcggcagag ttttgggtag
     8701 tcgagtttgg taaagccgct gtgccacagt ccggcggtca gccacaccat catcacgccg
     8761 cccatcatgc cgccgagggt aatcaggtgc aggggcgcgg aggcgggcag gttttgtaat
     8821 ttcgccgcgc ctgtccacaa atagcctgcg gcggcaaaga gctggagcag gtaataagtg
     8881 cggacgtagt gtttacgtaa gagttcgtga tggtgaagct cacgcagctt ggcgagcagg
     8941 atgaagccga cggcgagcgc ggtaaaaccg gcggtttgcg cgggcagcca aagttcggcg
     9001 gcggcgtgca agagcaggaa agtaatggcg atgtttttat aaacgatatt tggaataaaa
     9061 acagggtctt tcagacggca ttctttcagg gcttccgcgc ccaaaagaat actgacgcgc
     9121 acggatacga acatcaccgc cgccatattt agatgcactt gcgcgcgcaa caggttcaag
     9181 tcgccggttg ccgcgtatgc cgtctgaaaa acggtgaacg cggcaagcag catcagcagg
     9241 gcgaagttat ccgtgttgcg ggccagccaa atcaaccaag cgcagaacag caataaaacc
     9301 agccaataag ccgctacaaa gaatgcggca agttgcggta aaaacggcaa taaaacagcc
     9361 gcaacaagca acaacaccgc catcaaagta gtggcaggtt tcaggttgcc cgaaaaaccc
     9421 gtccagtcca acaaagccgc agtcaggaaa ccaccatatg ctgcaggcag catgagttct
     9481 aagaagattt ggcggtgcag gacgacgaca ccggggctga tgaaaaacac caacgcgccg
     9541 agtatggcaa gcactgtcgc gccgacgcaa aacgggcgca tagcaacagt atttttcacc
     9601 ccgtcgggca aaaataccaa aactcaaatc aagccgtccg gataccgttt tcggcg
//
LOCUS       DAWKQB010000069         9652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.069, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000069 DAWKQB010000000
VERSION     DAWKQB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.069"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(228..431)
                     /locus_tag="WJL14_001250"
     CDS             complement(228..431)
                     /locus_tag="WJL14_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ7656317.1"
                     /translation="MELGYTPYNLRNRCKLIQAELAQIVGVKHYIQVGRWEAEPDTET
                     RRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(443..724)
                     /locus_tag="WJL14_001251"
     CDS             complement(443..724)
                     /locus_tag="WJL14_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656318.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(762..965)
                     /locus_tag="WJL14_001252"
     CDS             complement(762..965)
                     /locus_tag="WJL14_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656319.1"
                     /translation="MNIIEQEMKHSSLRKVDVEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1045..1617)
                     /locus_tag="WJL14_001253"
     CDS             complement(1045..1617)
                     /locus_tag="WJL14_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEZ7656320.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            1596..1907
                     /locus_tag="WJL14_001254"
     CDS             1596..1907
                     /locus_tag="WJL14_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656321.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSVLSSVLPASLRRLRVGLTLTASSLRYSVARLMPNSCACCLRYAERAPRSIAS
                     A"
     gene            complement(1929..2804)
                     /locus_tag="WJL14_001255"
     CDS             complement(1929..2804)
                     /locus_tag="WJL14_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ7656322.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTQTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(2849..3139)
                     /locus_tag="WJL14_001256"
     CDS             complement(2849..3139)
                     /locus_tag="WJL14_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7656323.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3151..3330)
                     /locus_tag="WJL14_001257"
     CDS             complement(3151..3330)
                     /locus_tag="WJL14_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ7656324.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            3443..3820
                     /locus_tag="WJL14_001258"
     CDS             3443..3820
                     /locus_tag="WJL14_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656325.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            3849..4094
                     /locus_tag="WJL14_001259"
     CDS             3849..4094
                     /locus_tag="WJL14_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656326.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            4384..5295
                     /locus_tag="WJL14_001260"
     CDS             4384..5295
                     /locus_tag="WJL14_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656327.1"
                     /translation="MFDLEVALRRVLSAAPTVQDTQIEQYNQLTQIMHRTLDPKDVKG
                     NSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPPSGYSFLRKEAFLYIVGHHV
                     IFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFKAVGNCDKLSLIQQHGVKSI
                     LLDASAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSDEELEAQSEIHINLEVLLNG
                     NSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRYDEA
                     NSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            5292..5774
                     /locus_tag="WJL14_001261"
     CDS             5292..5774
                     /locus_tag="WJL14_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656328.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            5864..6367
                     /locus_tag="WJL14_001262"
     CDS             5864..6367
                     /locus_tag="WJL14_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ7656329.1"
                     /translation="MSDKFNQFINRVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGVIGAVSLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            6381..6554
                     /locus_tag="WJL14_001263"
     CDS             6381..6554
                     /locus_tag="WJL14_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656330.1"
                     /translation="MSKKSLIALMTAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKKRHK"
     gene            6479..6817
                     /locus_tag="WJL14_001264"
                     /pseudo
     CDS             6479..6817
                     /locus_tag="WJL14_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6810..7220
                     /locus_tag="WJL14_001265"
     CDS             6810..7220
                     /locus_tag="WJL14_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ7656331.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMAALKKLDALIYGKEVRQKERNSSQG"
     gene            7225..7974
                     /locus_tag="WJL14_001266"
     CDS             7225..7974
                     /locus_tag="WJL14_001266"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ7656332.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIWRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQTRPTFDLAGEI
                     LKGAPEKPRSSEKPILHSDQGWQYQMFFYSGLNLNQDRAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8295..8657)
                     /gene="ssrA"
                     /locus_tag="WJL14_001267"
     tmRNA           complement(8295..8657)
                     /gene="ssrA"
                     /locus_tag="WJL14_001267"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8720..9325)
                     /locus_tag="WJL14_001268"
     CDS             complement(8720..9325)
                     /locus_tag="WJL14_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ7656333.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2357 a         2195 c         2457 g         2643 t
ORIGIN      
        1 aggattaagg gggtatttgg gtagaattag gaggtgtttg gggcaaaaac agccgaaaac
       61 ctgtgtttgg gtttcggctg tcgggaggga agggaatttt gcaaaggtct cagagtgagt
      121 ttattttggg gcggcggcgg gtcgggggca agcggcgtgg ggcttttagg tttgggggtg
      181 ggtaaaaaat gccgtacccg gcgggcaggc ttcgcactct gaaggtttca gacggcgttt
      241 gttttttctg tccaatcgag gaactgccgc catttttcca gcggcatatc ggcccgccgc
      301 gtttcggtat cgggttcggc ttcccagcgg cctacctgta tgtagtgctt caccccgacg
      361 atttgcgcca actcggcctg tatcagtttg cagcggttgc gcaggttgta aggtgtgtag
      421 ccgagttcca tgtcgttgcg gtttattttg tttcgcatat ttttttgact gcccggcggc
      481 aggtttcggt aaggacggcg gcaaatcggg cttcgtctgc cggttttccg tcgaaaaaaa
      541 taaaatcgta aagggtgatg ccgttttcgg aatggcggta atgcccgccc gggcattcgc
      601 tgtctgtctg cctgtggatt ttggcaatca ggcggggata gcggcaatgg gtaatgaaac
      661 ggcttgcgcc gtcttgcccg ataatccatt cggggtagcg gttgaatagt gcggctctgt
      721 tcattttgtt cgggggataa agcccctcgc ggggcttgtg gtcaggcaaa tttgaatatc
      781 agtgccaaca cggcggcgat ggcggtaacc agcccggtgg ccgccatcat gggataccat
      841 cgggattctt gggctatttt tacggattcg gcgttgattt tgtgcgcgtc tgcaatgatt
      901 ttggcgattt cgacatctac ttttctcaga ctggagtgtt tcatttcttg ttcgattatg
      961 ttcatcgtac ttcctttcgt ttttggcggt tgccgccgct tgtcggatgg caggatgtct
     1021 gccatgtgta tatattgata ccttttaggt tttattgcaa gtgttttggg cggcggcttc
     1081 gtatgcttgg cggtggcggc ggctgtacca ttcggcgagt tcgcgccgct cttggaggcg
     1141 gtagctgtcg gcggtcaggt agtggtgcag gcttgagaag ccggcgcgtt ggagggcggc
     1201 gcggctgatg tggccgaggg cgaggtctgt tgtgagggtg tcttttccgg cggtcaggct
     1261 gatgttggtg aagaggtggc ggaatccgtg cattgtgtgt ttggatttgc cgggggtgct
     1321 gccgtcatag cccagtcgtc ggatggcgtt gtgggcgaat ttgatgctga tgtggtcggg
     1381 atggggggcg ggtttgcgcc gtgggcggat gccggggaaa aggtggatgt tgtcgccggt
     1441 ctgtgtgtgc agctctcgga gtatttctac cgcccagtcc gacagtggga cggtaaaggg
     1501 gtgtttggtc ttcatgtcgg cggcggggat gtgccataac cgggcggtga ggtcgatgtc
     1561 ttgccagcgg gcggaaagca gtgcggacgg acgggatgac ttccccggtt tccccatcaa
     1621 tccgcgccgc ctcgattttt ccgttggcgc gcagcatttc caccgcctgc cgcaccgaca
     1681 tcaaccgctt gccgttgcgc ctcatcgcct ccaccaaaac cgccgaaatc aatttggctt
     1741 cttccgtttt aagctccgtc ttgcccgcat cgctgcgccg tttgcgcgtc ggcttgacgc
     1801 tgaccgcctc cagcttgcgg tatagcgtgg caaggctgat gcccaattcc tgcgcctgct
     1861 gcttaagata tgcagagcgt gcgccgcgtt ccattgcttc cgcctgattc tcgactgcct
     1921 taagacgctc aatcattgcc ggattcatcg ccttctcccg tttcaccgcc caaccattcc
     1981 ggcacattgt ctgtcggtgc ttcagtcggc agggcatagc tttcgcgcag ttgctcgcag
     2041 tccaaaataa tttgattgag cgtgccgacc atctttgcct gatggctgat cccgtgtgcc
     2101 tcactgtgcg cattaagttg gtcgaacaaa tctttcagac ggctcacttg actgcggata
     2161 ccgacctcaa ggcttgttaa ctgcatcgcc aactcgctgc ctacgtcttc cgccttcggc
     2221 tctctgacaa cggtttgctt cttagccagc ttctcggcca gctcatcaat tttggctgtt
     2281 tgggttttca tcacttcgtc tttggcggcg aggttttcgc ggctttcgcg cagggcgacg
     2341 cgcagctcgc gcaccgtcat tcggtccaca tcgtcaaagg tcatgccgtt gacttcttcc
     2401 ccttcggcca aacccaccag cgtaacgtct tcttcgacca gcagctcaag cagcttcgac
     2461 ttgcccaaat ccatcagctt cggcgcggct ttctgcattt gcggggtcgc aaagcggcga
     2521 gtggctgaca tcagacgtga tgtttctgcg atacccagcc caaattggct tttcacaatt
     2581 tccataaacc gtccgtgttc cgtatgctct tttaaaataa tcagcgcacg tcccagttcg
     2641 aacatgcctt ccatcgtctg ccgtactgct tgacgaccgc gttcaaccca acgctcttcg
     2701 ctataagtct cgccgttacc ccactgctcc atcaccagca cactgcgcat cgctgcctga
     2761 ttgcttactt tgtcggttgc gataatttca atttctgtat tcatttttta tctccaaagt
     2821 ttccgacgtc ggaaactttc aaaatccgtt aatccacatc gacccgcttg ccgatttcgg
     2881 caatcttgct ttgcagccgt tcatgctgct gcctgaaccg ctctgcgatt tgcagggttt
     2941 tgatgccgta ggcgtagttg ccgttttcaa gtttgatgac caatcccgag gcaaccaaat
     3001 catcaatatc cctgctgact tgcgatggcg tcagccccag tccgaccgat aaatccttat
     3061 tgctcagacc gataatcgga tgctcgtcaa gcgcgataaa gaccctcaat agccgttgta
     3121 cccttttact ttctgccatc cgcatcctcc ttatttcagt cccagcttct tggcaatttc
     3181 atgccccttg ccgtaattgc ctttgcgctg tccgccgacc accagataca catcgcgcgg
     3241 cttaaagccg ttctctcttg cccactgcgc cagcgtttgg ccgttttggg caaaattttc
     3301 ttttagtttt tcaacagtat agccatagat agccctttcc cattgtgata aaatagtgaa
     3361 ctatttatgc actagaaatt aattagtgca ttaccaatgc gtgaattatg agattttaaa
     3421 tctcttttgc aaggattatt ttgtgatttc taaatctctt tttggtaatc gtttgaaaga
     3481 aaaaagaaag tttttagggc ttacccaagc acaagctgct gaaaaagcag gtattgagcg
     3541 cgaaacatgg ggcaaatacg aacgtggcgt atttatgcct agtggagatg tattgctctc
     3601 ttttttgaat atgggaatag atgtgagcag cttatttgct gctgaacagg gaattaggcc
     3661 gtctgaaaat tctgaaatta gtaaggaaga gtcagagtta ttagggtatt accgccaagc
     3721 cagcgataac ggcaaatttg tcattctcag tgtggcaaaa ggcgcagaga aaaaagcagc
     3781 cgaaaatact caagtttcag ccacgcataa aacagcgtga tttttaaatc caaaatggag
     3841 acagtcttat gtttgacttt gaacaacaaa tcaaatgggg tgagcgagcg gaagaaatcg
     3901 ttaaagaggc agccacccag aacaatatcg aaattcccga acctttggct tcggcattag
     3961 ctaaggcggt aaaagtgcat tatctaagcc aagctggtgt gttttctttg gtagaagcct
     4021 atgcagacac ggtaaatcca actgaaaaag aagtagatta ccaagcaatc ggcaaagagc
     4081 tatttgaaaa ataaaaaatg ccgtctgaaa ttttcagacg gcatttagga cactccgaca
     4141 gtcgggctgc tccggtgttc tgaaacggat attaagcaat tgaagcggcc gtgcatttta
     4201 aaacgcatta aaaactaccc cgacatacag gtcatcgggc aggtggttca ggtgttgaaa
     4261 gatttgaatt gatgccgtct gaatttattt tttcgacaca gatattaaaa gagaaaggac
     4321 tgtacaaatg gcaaaaaaac tactaaaact attcattatt tacgtgcgca gcaaaaccat
     4381 agcttgttcg atttagaggt agcattaaga agggttttgt cagctgctcc tactgttcaa
     4441 gatacacaaa ttgaacaata caatcagctt acccaaatca tgcatagaac tttggatcct
     4501 aaagatgtaa aaggcaattc aattggtgga ttactgatac acattggcag cggtacaaaa
     4561 gatgaacata tccgcactat gagcaataga cctgtacagc aagatgatca tggtggaaca
     4621 caagctcctc caagtggata ttcctttttg cgtaaagagg cttttttgta tattgttgga
     4681 caccatgtaa ttttttgcgg acatggtttt ttgtcagcat caacggttgc ttcttattta
     4741 agtttactta gcaataaatt gagagagaac aatccggata ttgttcaatt taatattgaa
     4801 ttcaaagcag taggtaattg cgacaagttg tcattgatac aacaacatgg ggtaaaaagt
     4861 attcttttag atgcttcggc atatcaatta tccagaaaca gactgtatca agatagccgt
     4921 tcaaccattg ctaaagcatt agggaaagtt ggtagtgtat ttacaagcga attgagtgat
     4981 gaggaattgg aagcacaatc cgaaattcat atcaatttag aagtcttgct aaacggtaat
     5041 tcgagagcca gtattgaagc gcaatcgctg atgcaggagc aggcagagga aattattgat
     5101 gatgaaacgg ttaatcaagg attttccatt accacgcaac aaggtgaagt gattcagccc
     5161 tctgatgtaa aactgtctaa atctgttaga ataggacgat acgatgaagc caactcgtta
     5221 ctccctgact cggcatttac agcaatcagt gaatatttcc tagaactcca aagcagaaac
     5281 ttaactgaac aatgaaaaaa tatctgccct tactacgttt tttgttgatt gcttgtatat
     5341 cagcattttt agcatggaaa gggcaacctt ttgttcatgg gaacgagaag gccgttgatt
     5401 taattattaa cgtatttgca atcttagccg gttttttaat tgccattatg acattgttta
     5461 gtgatatgag gtttgatgaa gatgcaaatt ggcgacaaat tcaaattcgt gaaggcgtac
     5521 aagaacaacg gtatataaaa cattccttac ttttttacac atatcttgcc gtattagttt
     5581 gtgtttttat tgtcatcttg ttggctcata aagaagaata caaaaatgga ccagccattt
     5641 tttggcttga acggagctat ttatttttag cctgtatttc cattttttat tctgtattct
     5701 tacctgggaa tttaattaaa agccgaaaag aagagtttaa aaaattaatg gaaaagaaga
     5761 aacccaaaat ctagaacgag ttttaacccc ctttaaaatc ccattcagac gaccttttct
     5821 aaaatccctg tattgatttt taattcaata cagggatttt tccatgtcag acaagttcaa
     5881 ccaattcatc aaccgcgtcc tctctcacga gggtggttac gccaaccatc ccaaagaccc
     5941 cggcggcgaa accaattggg gcatcactaa gcgcaccgca caggcaaacg gctacaacgg
     6001 ctccatgcgt gccatgacgc gtgaacaggc aatcagcatt taccgtaaag cgttttggga
     6061 gcgttaccgc gccgaccaaa tgccggaagc ggtcgcgttc caattttttg atgcctgcgt
     6121 caaccacggt tacggcaatg ccgcccgtat gctccaacgc gccgcaggcg taccggacga
     6181 cggcgttatc ggagcagtca gcctcaaagc catcaattcc cttcccgaaa acgacctttt
     6241 attgcggttc aacgccgagc gtctggtctt ttataccaag ctcggtacgt tcacctcttt
     6301 cggcaagggc tgggtacgcc gtgtggcgca aaacctgatt cacgcgtctg cagataacac
     6361 tgattaaagg gagataaacc atgtcaaaaa agtcactcat cgccctaatg accgcagcca
     6421 tgcagcccga tttcagccac agcgacctag gcattcgcta cgccatgccg actcagggat
     6481 gttggacgca agcccaccgc aagagcgggg tagccgccgc gaaacgcgca gccaaaaaaa
     6541 agcgtcacaa ataaccgcct ttttccgatg gctgggcggc ttggtctcta atccggccac
     6601 aggaaaaatc agccatacca aactatgggc aaacgtcgcc gcagccgcca tgacttggaa
     6661 gttcgtgcag gcggcggacg cgcccgaatg gctctagtgg gcttatggcg cattggtcgg
     6721 cgggtatgca ttaatcaaac gcggcatcgc ggcgattccg cagttggcag aaatcaaaaa
     6781 atccgcgaat gcggaagaat ggagcggcaa tgattgaatt tgtccgagcc aaaaaacggc
     6841 tgctttgggc atttgtgctt ttgcttgtgt ggacgtgcgg ttaccgatac gccgccgaca
     6901 aggctgaagc ggaacaaacc gccctgattg ccacctatcg gcattcttct atggttgcgg
     6961 cggaacaata tgccttgcag cttaaaaaag cgcaggacga aaggcagcgg tggtacgact
     7021 tttcccaaaa acaaggaaga aagcccgtga aaaaacagta tccgccgcaa acgaaaaaag
     7081 ccggctatct gaaaaccaag gaagaactgc ttgcggaatt ggcttgcctt aaagcggaaa
     7141 tggctgccct aaaaaagctc gatgccttaa tctatgggaa agaagtgcgg cagaaagaac
     7201 gcaactcgtc gcagggttaa ggcaatgcca tccgttgaac tgctgttggt gattgtcgga
     7261 ctgccacgcg gcaccttcta ttaccaattg gccgtccaat cggcagaaga caaatatgcc
     7321 gatttgaaac ggcatatcca tgatatttat cgacgacata agggaagata cggctaccgg
     7381 aggattgcgg cagccatccg tcacgcagga acaccggtca atcacaagaa agtcagccgt
     7441 ctgatggcga agacggggct gaaggcagtg atatggcggc gcaaataccg ctcgttcaaa
     7501 ggagaagtcg gcaaaattgc gccgaatatc ctgcgacgct gtttccatgc agaaaagccg
     7561 aatgagaaat gggtaacgga cgttaccgag ttcaatgtag gcggagaaaa gatatacctt
     7621 tctccgatta tggatttgtt taacggggaa atcgtcagtt accgtattca aacccgcccg
     7681 actttcgatt tggccggcga gatactgaaa ggtgcgccgg agaaaccgag atcgtctgaa
     7741 aagccgatac tgcattcgga tcaaggttgg caatatcaga tgttttttta tagtggatta
     7801 aatttaaatc aggacagggc gatgaagccg cagacagtac aaatagtacg gcaaggcgag
     7861 gcaacgccgt actggtttaa atttaatcca ctatacaaca acgatagaat caagttgaaa
     7921 ttaaaagggc tgagccttgt tcagtacaga attcagtccc tgaaagccgc ttgattaaac
     7981 tgtccgactt tttggggtca gttcggcttc ggcatttttt tatccgtttg ggggtaactt
     8041 gtttggaaag ctgcaagctt ataaataaag gattacattt aagttttggg taaccttttt
     8101 aaaaaaatgc gtgatgactt ttgcattctt aaggcgtttt ttggggtaat tcgtgaaaag
     8161 agttacccca aaagttaccc cataaatggc gaaaactcaa gcatacgcca gcatattcaa
     8221 ggcaacaaaa aagccttgaa actgttgaag ttcaaggctt ttttgtgttt caggatactg
     8281 ctgaaaatag ggtatggtgg aggcgggggg aatcgaaccc ccgtccgaaa gtcctctaca
     8341 aagcgttcta catacttagt tgtgtctatt tgaaaatctt atttccatca tgccgaccaa
     8401 caggcctttt ggaaaccagt taccttaaat ctcatttcct gccaaataac ccggcaggaa
     8461 accagtcaat gtaagatgac gttgcggtgg ctttcgccac acagcccatt gaccgactgc
     8521 tgcaacggct agccttaagc ggctaaagcg taagtttcgt cgtttgcgac tatttgaatt
     8581 cagtgtttta cgggaatctg agaccccggt atgcccgcat ctgcttcgca acccccgtcg
     8641 aaaccaaggt cgcccccaga aatggtttgc aaattatacg gatattgtgc ggtgctgcca
     8701 agtctgtcgg agaaatttgt cagtcttgct gccttaattt gcgtttgagc aggatgcgga
     8761 cgcagccgtc gttgccttcc cggggttcgg cgtaggcgag tacatcgggg tgttgcatca
     8821 gccagtttcg ggtcatattt ttcagaacgg gtttgtagcc tttggaacct aatccgctgc
     8881 cgtggatgat ttcgccgcat acgccgcgtt tttgggtgaa tgcgatgaat tcgttgagga
     8941 ctttttgggc ttcttcctgt gtgtagccgt gcaggtcgac atcggtaacg acgggatagt
     9001 atccgttttt caggcgttgg atgtcgtttt ttccctgtcc gtttttgctg aagctggcgg
     9061 gcgggtcgtt gtatgtgctg ccgatgtaga agtagttttc ttcgtcggcg cggttgtctt
     9121 tgggacggac tttgatgggg gttttgtcgg gcggcgcata atattgctgc cggtttttta
     9181 atggggagac ttgtccgact gcttgtgaaa aatcgaaatc ctgttcttgt ttttgcttgt
     9241 ttttttcggc tgcctgtttt tctgccgctt ctttttgggc ttgtttgccc agttgtttga
     9301 ggatgttttg gaagtcggta ttcatatttt ttcctgttat ttgtccgatg gctgtttcgg
     9361 gcggggtttt aatttgccgg aatgtttgcc aatcggggga ggatgatttt gttgccgtcg
     9421 tatgtttttt gaaagtgtga ttgtatatca aaaagaaatg cggcaaccgt cggcagtgtt
     9481 gattgccgga aatgcgggcc ggtcgaaccg atatgcccga acgcctgata aagttttaaa
     9541 aacctgcctt gcgaagcagg ttaatggttt tgccaatttt gaattgccgg aaacgcgaaa
     9601 cacggaaatc tgatgtttta taaatgttcc gatacgaact gcaaaatatt gg
//
LOCUS       DAWKQB010000070         9602 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.070, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000070 DAWKQB010000000
VERSION     DAWKQB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.070"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..546)
                     /locus_tag="WJL14_001269"
     CDS             complement(<1..546)
                     /locus_tag="WJL14_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656334.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIP"
     gene            complement(578..961)
                     /locus_tag="WJL14_001270"
     CDS             complement(578..961)
                     /locus_tag="WJL14_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656335.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1330..2034
                     /locus_tag="WJL14_001271"
     CDS             1330..2034
                     /locus_tag="WJL14_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ7656336.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2098..2664)
                     /locus_tag="WJL14_001272"
     CDS             complement(2098..2664)
                     /locus_tag="WJL14_001272"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ7656337.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2730..3146)
                     /locus_tag="WJL14_001273"
     CDS             complement(2730..3146)
                     /locus_tag="WJL14_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656338.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3198..4097)
                     /locus_tag="WJL14_001274"
     CDS             complement(3198..4097)
                     /locus_tag="WJL14_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ7656339.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJL14_001275"
     CDS             complement(4128..5585)
                     /gene="der"
                     /locus_tag="WJL14_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ7656340.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENADDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5739..6368)
                     /locus_tag="WJL14_001276"
     CDS             complement(5739..6368)
                     /locus_tag="WJL14_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ7656341.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFAVLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELAKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJL14_001277"
     CDS             complement(6369..7664)
                     /gene="hisS"
                     /locus_tag="WJL14_001277"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ7656342.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNFLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGEGADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7758..8300)
                     /locus_tag="WJL14_001278"
     CDS             complement(7758..8300)
                     /locus_tag="WJL14_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ7656343.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVGQIRQARAE"
     gene            complement(8472..8663)
                     /locus_tag="WJL14_001279"
     CDS             complement(8472..8663)
                     /locus_tag="WJL14_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656344.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8696..9232)
                     /locus_tag="WJL14_001280"
     CDS             complement(8696..9232)
                     /locus_tag="WJL14_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656345.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLK
                     KDEIFKNIERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDQDKLFKSRETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9400..9600)
                     /locus_tag="WJL14_001281"
     CDS             complement(9400..9600)
                     /locus_tag="WJL14_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656346.1"
                     /translation="MQLICADWTGIGSFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2252 a         2321 c         2436 g         2593 t
ORIGIN      
        1 cggtattttc ttaccgtatt ctgcgattgc ctgtcggaaa atgccgatca acctgcctat
       61 aacggcattt tcgccaaatt cgttcagaca gttttcccta agtcgggcag gttcaaaatc
      121 agagtggtgt tcatacattt tgatgagtgc gtcggcaagg gcatcgtcgt cgtcaacagg
      181 aaccaaatat ccgttgccgt ctgaaacaat agattccgca ccgccgcagc gtgtcgcaat
      241 gacgggcaat ccttgggaca gtgcttcgat atagaccacg ccgaaggttt cggtgcggct
      301 ggcaaggacg aatgcgtcgc tgttcctcat caaatccaag actgcttcgg gctgcaatgc
      361 gcccaaaaat gtaacggcat gggtaatgcc caagtctgcc gcctgctgtt tcagccgctg
      421 ttcttcctgt ccgctgccgc cgatgttcag gcgcagttgc gggcattgtg ccaacgcccg
      481 ggcaaaggca gtgagtagga catcgtgtcc tttgagacgg cgaaggtgcg agacggtgca
      541 gaacacgaaa tgcggtttgt tgtttttttc tctttgttca aacggtcggt tgaatattcc
      601 gcccagtatg ttgggggagg tactgccatt cgcagccgta tttgtgttgc aggacgtgtg
      661 cgaaatggcg gctgacagcg agcagtgcgg aggcgtgcgc cgctgctttt tccatcggct
      721 gccattggtg gggacggatc agaccgcgcg tgatggtgct gctgtgttcc gtgacgacat
      781 aggggatgcc gtatttttgg gaaatttttt gggcgagtat gccggcatag ttcatgcggt
      841 gggcgtgaat caggtcgggc agcccgtttt cgcggatgta gtgtttgaaa gctttcaaac
      901 ccgcacacac ccagcggatg cagtcgatgt cgatgaacgg aaagcggggg aagaaatata
      961 tgccgtgcca tgcatagatg tccaaaccgc tttgccgata ttttgcaaaa ccgtaaggcc
     1021 ggtcaggatg cttgctgttt ctttccgcaa gtagcggaac atcggtgcaa gcacggcggt
     1081 tttgatgcct ttcctctgca atgccagtgc ctgattttga aaaaaatccc gtccacatcc
     1141 tgttcggatt gcggatacca tgaggggatg acgaggacgt gcaagggttc gggcatagtg
     1201 ggattccgta tcggaaaggc ggttattata agacagacgc agaccgaata tttaaattgt
     1261 tgccttacgc taatgcaatt tagcgcgccg atatgctaga ttggcggttt tataagtaag
     1321 gatacggata tgttgcgttc tattttggcg gcttccctgc tggcggtatc ttttccgacg
     1381 gcggctgaag cattgaatta caatattgtc gaattttctg aatcggcggg tgtcgaggcg
     1441 gttcaggata caatgtccgc acgtttccaa gtgacggcgg aaggacggga caaaaatgcc
     1501 gtcaatgccg agtttgttaa aaaattcaac aatttcacca gaaaatcaaa aaatggtagc
     1561 tttaaaaccg aattggtatc gcgcagtgcg atgccgcgct atcaatatac caacggcaga
     1621 cgcattcaaa caggttggga ggagcgtgcg gaatttaagg tcgagggtag ggattttgat
     1681 gcgttgaacc gttttattgc cgatgttcag gcagatgccg cgttggaata tacggatttc
     1741 catgtgtcgc gcgaacgccg caacgaggtc atcgatcagg tcagcaagga tgccgttttg
     1801 cgtttcaagg cgcgtgccga aaagttggcg ggcgttttgg gtgcgtccgg ttataaaatc
     1861 gtcaaattga atttgggaca catcggcagc catatcgcgg gagggggagc tgctcaggca
     1921 aaaatgcttc gtgccatgcc gatggcggca agcgtcaata tggagggtgc ggattccgcc
     1981 gcgcctggtg tggaggaaat cagcatcagc gtcaatggga cggttcagtt ctgatttgag
     2041 gtgaacggca aatgccgtct gaaacccgac gataagggtt cagacggcat ttatatttca
     2101 ggctttgggc agggtaacgc cggtttgccc catatatttg ccgttgcggt ctttgtatga
     2161 ggtttcgcag atttcgtcgc tctcgaagaa gaggacttgc gccacgcctt cgcctgcgta
     2221 gattttggcg ggcagggggg tggtgttgga aaattcgagg gtaacgtagc cttcccattc
     2281 cggttcgaac ggggtaacgt tgacgataat gccgcagcgg gcgtaggtgg atttgcccaa
     2341 gcagacggtc aggacgttgc gcgggatgcg gaaatattcg accgtgcgcg ccagtgcgaa
     2401 ggaattgggc gggatgatgc agcagtcgtc ttcaacggta acgaagtttt tcgggtcgaa
     2461 gtttttggga tcgacgatgg tgctgttgat gttggtgaag attttaaatt cgtttgcgca
     2521 gcggatgtcg tagccgtagc tggacgtacc gtaggagatg atgcgtttgc cgtcggcttc
     2581 tttgatttgg ttcggctcga aagggtcgat catgccgaat tcttcgctca ttcggcgtat
     2641 ccatttgtcg gacttgatgc tcataatgtt ttccttgttt cttgcagtgt tcggacaaag
     2701 cattggggga tgccgtctga aaacggggct tatttgtttt tgggcagctt cacttcttta
     2761 atcatgccgt tttcgcattt cataatgaag agggtttcgc cgttttgcga gacggtaaat
     2821 ctgccgtgtg ccaagccttt tttgaatgtg cccgagagta ccatgttgcg gaatttggta
     2881 ctgtcggaat tgaacggttc gataaatatt tcgcggttgg cggcaacggt ataaacgcct
     2941 tgtccgtcga atttgccatt tttaaacgaa ccggtatagt tgcgcccgtc ttggcagcgc
     3001 catgtgcctt tgccggcggg tttaccgtct ttgccgacat cgccttcgta ggtgcagccg
     3061 ggttcttgat aggaagtcag aacggcggac gcggcgggga gggcgaacat catggcgggc
     3121 agtaggaatg cgagatgttt aagcataagg gttattccat tggattttgg ctgacggtat
     3181 aaaagccgtc tgaaaaatca atcttgccag ccgcccaaat aggaaaccag ctcttccaac
     3241 atgatgctga cggattccgc catcaggatt tgcgaggcga aggcgagacc ggcggcatcg
     3301 tcgccgttgc tttcggcttc ttcctgcaat acgtcgaggt attggatgcg cttgagtgtg
     3361 aagtcttgcg tgaggataaa ggcaatttgt tcgcgccaca ccaagcccag ttgggtaacg
     3421 gttttaccgt ttttgacgtg ttgaaccact tcgtcggcgg ttaaatcttg tttggatact
     3481 ttgacgacgg gaacaatatc gcccgtgcct ttgagttcgc aatcgctgtc caattcaaaa
     3541 ccgccttcgc aatgcccttg caacagccag ccggtcatca gggaagaggg cgattgtttg
     3601 gtattcggca gcgaggcttc caaaccgccc aaagcttcgc gcagcttggt caggatgttt
     3661 tccgctttgg cggaagccgc gttattgacg agcaggtagc cgtggcgggt gttaaacacc
     3721 gcttctgtac ggctgctgcg ggtaaacgct cggggcagca ggtcgtctgt aatttgctct
     3781 ttaagctctt gtttttcttt gcgaccgaca ttgcgggctt cattgttttg gatttccgct
     3841 accttctctt ccaaaatatc gcggatgacg ccggcaggca ggactttttc ctcttttttc
     3901 agggcgacgc gcaaggtaaa gtcggcaggg aaaacgagtt ccggggagaa tgaaaccggt
     3961 gcggtaaagc cttcgctgaa ccagtctaag ccttggcaat gggtaaattc agcttcagca
     4021 agtttgtcgg caagtacgtc cgcctcaggc agcttttctt tgttgagcgg ataaaaacta
     4081 atctgcttga accacataat gtttcctgtt gtttgaaatg tcgggaatta tttgctgaat
     4141 tgttttttca cactgacttt ggttttcttc ttgaagcggt ttttctcttc tttgcggcct
     4201 tcgcgtttct caatacggtt gctcaggctg acgcggcgca gcggtttttt cttcggtttg
     4261 tcgtcggcgt tttcatacgg gttttccgaa acattgtatt gaatccgcaa cggcgtgcct
     4321 tgcaggttga aggctttgcg gaacgtttgg gtcagatagc gcgtatagct gtcggaaatc
     4381 gcgtgcagcg aattgccgtg taccacaatt acgggagggt tcatgccgcc ttggtgggca
     4441 taacgcattt tcggacgcac caagccggca cgcggcggtt gctgacgctc gattgcgctt
     4501 tgcaggacgc gcgtgatttt cggcgtcggc atcttaatca tcgccgcgtt gtaggcagcc
     4561 tgaatgctgt caaacaaacc gtctataccg cgctctttca atgcggaaat aaagtggaac
     4621 ttggcaaaat cgaggaaata cagtttgcgg ttgatatcgc gtttcacttg ctcgcgacgt
     4681 tcttcgctga tgccgtccca tttattgacc gccaccacca aagcacggcc tgcctccaaa
     4741 gcgaaacctg caatcgtcgc atcttggtcg gcgatgtcct gctgcgcgtc caataccaaa
     4801 acagcgacgt ttgccgcttc aaccgcctgc atcgctttga taacggagaa tttttccact
     4861 gcttcatcca ctttgccgcg acggcgcaca cctgcggtat cgatgatggt aaacggtttg
     4921 ccttcgcgct cgaaatcgat atggatactg tcgcgcgtcg tacctgccat atcgaaggcg
     4981 atgacgcgct cttcgccgag aatggcgttt accaaggttg atttaccgac atttggacga
     5041 ccgataacgg caaaaacagg atgtttcgcc tcttcctctt ctttttcagg ctcagggaag
     5101 gtttccaaaa tatcttcaat cagataatac acgccgtcac cgtgcgcacc tgaaataaca
     5161 taagggtcgc ccaaagcaag ttcgtagaac tcggcggcaa gcacggccct gttgccgcct
     5221 tctcctttat tcacggccaa ataaacgggg cgcgggcttt ggcgcaaacg gtcggcaatg
     5281 attttatctt gcggcgtcag accggtacgg ccgtccacca aaaacacaac cgcatcggct
     5341 tcatcgacag cctgtaaggt ttgttttgcc atttcgtgca aaatgccgct gtccacaacc
     5401 ggctcgaaac cgccggtatc gatgaccaaa taaggtttgc tgccgacttt gccgtgtccg
     5461 taatggcggt cgcgggtcag gccgggcagg tcgtgtacga gtgcgtcttt ggtgcgcgtc
     5521 aaacggttga ataaagtaga tttgccgacg ttggggcggc cgacaagcgc gatggttggt
     5581 ttcatgattg agtctttctg tgtcaagtgc cgttcgggag aactgaacac gagcaggtgt
     5641 ccgttggaca cggtaaatag gttcttcacg gcaagaggcc ataaagaggg agggtaaaat
     5701 gccgtctgat aaataatgga cggcatttca gacggcattt atttcagcga atcaagtttc
     5761 atttgaacca attcgcgacc gacagaatct tgaggcattt tttctaaagc ctgtccgtag
     5821 ttttttaagg cttcctggct ttttccctgt gcggcataga catcgccttt ggtttccatc
     5881 agcagggggg cgaagtccgc ttcaaccggc gtgtcgagtg cggcaagcgc ggcatcgtat
     5941 tttttttgtt gcaacaacac aacgcccaga cgctgcgccg ccaacgcctg gatcaggctg
     6001 tctttttggt tggacaatac ccatttcaaa tggccttcgg caacatcgta acgctgcgcg
     6061 tcaaattcgg ttgctgccgc catcagcgtg gcttgggcgg cggaaatgga atgggggtag
     6121 ctttgttgga gcttggccaa ttcggcattg atttcgcttt gcggggcttt gttttgcgcc
     6181 ttttccacga tgtttgccag caccgccgcc gcttcctgat tttgggaagc cgcacggttt
     6241 tggtaaaccg tgtatcccaa gtagccgagt gccgccaaaa tcagcacggc aaacagccat
     6301 ttgcccgtgg ttttccaaaa atatttaaag ttgtccaact cttgttgttc ttccaaatgg
     6361 gctgccattt atgcgttctt ccattgttgt aaagtgtttg tcaaatcagc ggcggcgacg
     6421 gtttgctgac cgtgtgcgcc gttcatatct ttgagcgtaa ccgtaccgtt cgccagttcg
     6481 tcttgcgcga caatcagggc aaagcgtgcg ccgctgttgt cggctttttt catttgcgct
     6541 ttcaggctct gatagccgga atgctgcatc acattgaaac cttgcgcgcg caaggcttgt
     6601 gcgtatttca tcacctgcaa gtccgcccct tcgccttggt gcattgcata gacatcaggc
     6661 gcggcgttta cttccagcga gccgtattcg ctcaccaaaa gcagcagccg ctcgatgccc
     6721 attgcaaagc cgatagacgg cgcaggctta ccaccaagtt cctcaatcaa accgtcgtaa
     6781 cggccgccgc cgcacacagt tgcctgcgcg ccgagtttgt cggtcgtcca ctcaaaaacc
     6841 gtttgattgt aataatccaa accgcgaacc aagcgcggat tttcaatata ttggataccc
     6901 aaaccgtcca acatcgcttt gaagcgtgca tagtggtttt gcgaagcctc gcccaaataa
     6961 tccaccagac gcggtgccgc attgcagatt tcctgcaaat cagggttttt cgtatccaaa
     7021 acgcgcaaag gattggtttt cagacggcgt ttgctgtctt catccaattt atcttcataa
     7081 cgggtcagat attcaaccaa tgccgcacgg tgtgccgcgc gttcctcacg gttgcccaag
     7141 ctgttgattt ccaaagtcag gtattcgcgg atacccaatt tttcccacaa gtcggcagac
     7201 atcgcgataa tttccgcatc gatatccggc ccttcaaaac ccaaagcctc gataccgacc
     7261 tgatggaact gacgataacg gcctttttgc ggacgctcgc ggcggaacat cggccccata
     7321 taccacagct tttgcgggct gttgtacaga aagttgtgtt cgaccaccgc acgcaggcag
     7381 gaagccgtac cttcaggacg caagctcaaa ctcaaagaat cgtttgaatc ggagaaggtg
     7441 tacatttcct tgccgaccac atcggtttcc tcgccgatgg agcggacaaa caaaccggtt
     7501 tgctcgacaa tcggcgtacg gatttgctga taaccgtaag tgcgtgtcca gcggccgacc
     7561 gtatcttcaa acgcctgcca aaacgcagcc gtcagtttga aatctttttg ctcgacaggc
     7621 agaaggtcgt tcatgccttt gacggattgg attttttgtg ccatttcaag taagaatgct
     7681 taaatcaaat tgcgggcgat tatagcggat ttttaagggt ttgtgaggtt gggggtggtt
     7741 tgcggacggc atttgactta ctctgcacgt gcttgcctga tttgtccgac tgcaaactcc
     7801 gtctgccgct tgggatgatg tgtgaaaaac aatttatttg aaattgtctc ggcttttttc
     7861 ggcacgaccg ccaaaatctt acctgccaaa tttccctcac gggtttgcca agcatccaaa
     7921 aactgcgccc tgctcattga aacatgaccc agcgacgggt cggcaagcaa aaccgtattg
     7981 ccgtctatcc cgcgcaatac cgagaaatga tcatccttgc ggtatttcag atacacgatg
     8041 acggggattt tcaactgtgc gagctgctcg aaagacaggg catagccttt cgcttcaaaa
     8101 cccaaatctg gcataatgcg ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc
     8161 agctttttca acacttcttc ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc
     8221 gccaccgaag ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc
     8281 ctttctttcc aactctgcac tttgattttt ccgtaaacaa caggattatc gttaaacatc
     8341 ggtgcagcat tcaaacgata agataaagaa acgacaacac acgccaacag aaaaacatat
     8401 ttgaacttca tcatattgtc cacataaagg gcagcctgaa aatctttcag gctgcccttg
     8461 tcaaattatt cctagctttc ggcttttttg gcaaaccaaa caatccgatt acccgcataa
     8521 tactttccat ttattgaaat ccgacaagcc gcgcccaaaa aatgccatgc actgtcgatt
     8581 tccgcagcaa tctttgtacc gttttcttca aattccaaat attcacccaa taataaactt
     8641 gaaacagaac gcgtgggcag caagtgccca ccctacgctt attcaaataa tttgattaaa
     8701 taaagaataa agaaaatgca tcaggaacaa aattataatc tgccacctga ctcacaccgc
     8761 ttttaaagta aggggcatca aaatcaaaac cgcaaaaaaa ataatttttg cattgatttt
     8821 taatagattt aaaattcgaa tatagtgttt ctctagattt aaaaagttta tcttgatcta
     8881 tatatttgat gctttcccta tccaaaataa atatttcaaa cattaaaaaa tcattacatg
     8941 accaagccaa atcataaatt tgattcaaat gggtatcata aagataaaaa taataaggtt
     9001 tgggaacagg taaaatattt aatggaaaag gaggactaat tttcttaata tctgatgact
     9061 gatatccata tctttctata ttcttaaaaa tttcatcttt cttaagataa caagacattc
     9121 tgacaatcac aacctgttca tcggatatat tatctatttg catggcgcgt ataacacgcc
     9181 atgcctgatt aaaattagtc tcccttacct tagaatctat tatcttttcc aaagatgaaa
     9241 atgcccctta tcttgaactc tccagttaga acctggaagt ttcgtacctc ttaaatgtgt
     9301 attaatattt cttatagttt cattaaaatg ccacgcgctg ccgatttcaa cggtaatttt
     9361 cgtaccgctt tcttcaaatt ccaggtattc ccccattagc taacgcaaag aagcagacgc
     9421 catttcggct tcgttatgat aaacccgcct tccgttgata tagacttccg cccctgtccg
     9481 ctccaaattc caaaaattcc gtactgaaat ttccatatcc cgatattgtg cagaccatgt
     9541 tttttcgaag gttttcataa aacttcctat acctgtccaa tcggcacata tcaattgcat
     9601 ta
//
LOCUS       DAWKQB010000071         9587 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.071, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000071 DAWKQB010000000
VERSION     DAWKQB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.071"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..253)
                     /locus_tag="WJL14_001282"
     CDS             complement(<1..253)
                     /locus_tag="WJL14_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656347.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITGHNGKPDTKPNTAAKM
                     PSEAVGATILIWGSLQGRITFLNHPFDSQINSAFACQKQN"
     gene            complement(250..1305)
                     /locus_tag="WJL14_001283"
     CDS             complement(250..1305)
                     /locus_tag="WJL14_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7656348.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            complement(1411..1893)
                     /locus_tag="WJL14_001284"
     CDS             complement(1411..1893)
                     /locus_tag="WJL14_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7656349.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            complement(1940..3076)
                     /locus_tag="WJL14_001285"
     CDS             complement(1940..3076)
                     /locus_tag="WJL14_001285"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ7656350.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTILSTDSDTAFQEAKKLHQSL"
     gene            complement(3073..3618)
                     /locus_tag="WJL14_001286"
     CDS             complement(3073..3618)
                     /locus_tag="WJL14_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ7656351.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRSAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            complement(3615..5090)
                     /locus_tag="WJL14_001287"
     CDS             complement(3615..5090)
                     /locus_tag="WJL14_001287"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ7656352.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            complement(5201..5566)
                     /locus_tag="WJL14_001288"
     CDS             complement(5201..5566)
                     /locus_tag="WJL14_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656353.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            complement(5587..7497)
                     /locus_tag="WJL14_001289"
     CDS             complement(5587..7497)
                     /locus_tag="WJL14_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ7656354.1"
                     /translation="MNILSVENASFAVGHVALLDKTSFQLDSGEKIGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKEATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFTYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGTLQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            complement(7516..8160)
                     /locus_tag="WJL14_001290"
     CDS             complement(7516..8160)
                     /locus_tag="WJL14_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ7656355.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            complement(8324..9142)
                     /gene="opc"
                     /locus_tag="WJL14_001291"
                     /gene_synonym="opcA"
     CDS             complement(8324..9142)
                     /gene="opc"
                     /locus_tag="WJL14_001291"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ7656356.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHIGVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWLAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
BASE COUNT         2075 a         2089 c         2735 g         2688 t
ORIGIN      
        1 ggttttgttt ctgacaggca aaagcactgt ttatttggct gtcaaaagga tggttaagga
       61 aagttatgcg cccctgaagc gagccccaga taaggatggt tgcgccgacg gcttcagacg
      121 gcattttggc ggcggtgttg ggttttgtat ccggtttgcc gttgtgtcct gtgatgatga
      181 ttttgggcgc ggtttttctg ttttgatgtg tgaaatgccg tctgaaaggc ggttcagacg
      241 gcatagcggt catttttgtg cggtcaggcg gtcgaatatg ccgccgtcgg cgaagtaggt
      301 tttcatgatg ttgtcccatc cgccgaattt ttcttcggga gagaaggtgt ctaagtctgg
      361 gaagtcggct ttgtgtcttg ccaatacttc ggggttgcgg gggcgcaggt agagtgaggc
      421 ggcgagttct tgcgccggtt cgctccaaag gtattcgaga taggcgcggg cggttttttg
      481 cgtgcctttt ttcgcgacga cgctgttgac gacggcgacg gggctttcgg cggaaatggt
      541 gtagctcgga tagacgattt caaactgtcc ttgggtcagt tttttgctga cgtagttggc
      601 ttcgttttca aaagtgatga gtacgtcgcc gatgttgcgt tgtgtgaagg tggtggtggc
      661 ggcgcgtccg ccgttttcaa aaacgggggt gtttttgagg atggatgcga cgagtttttg
      721 ggcttcctgt tcgttgccgt tggtggtttt cagaccgtaa ccgtatgcgc cgaggaaggc
      781 gtagcgtccg ttgcccgagg ttttgggatt ggcgatgacg atgttaacgc cgtctttggc
      841 aaggtcgttc caatcgcgga tctgtttggg gttgtttttt cggacaagga aaaccatagt
      901 gctggtgtag ggcgcggcgt ggtcggggag ggcttgttgc cagccttttt ctaccagtcc
      961 ttttttttcg agcaggtcga tgtcggagga ttggttcatg gttacgacat cggcttgaag
     1021 gccgttggct acggataatg cctgtttgct ggagccgccg tgggactgtt ggatgctgac
     1081 ggatgtgccg gggtgttcgg attggtatgt tttgataaat aaggggttgt attctttgta
     1141 aaaatcccgt gccacatcgt atgaggcgtt gagcagggta atgttttttc cgtcggattc
     1201 ggtattggcc ggggcatttt gtccggacgg atggtttgaa tcggctgcgg ggctgcaggc
     1261 ggtgagcagg gctgcggtat agagtgccgg tgcgtaggtt ttcatatgct tgtcctgtcg
     1321 gttggtagat ggggcaactt tatacggctg tctgcgcttg tggaaataat gtttgatttg
     1381 aagattatca gttttggtta taaggacgga tcagaggtgt ttccgcatca gttcgcattt
     1441 gattttgatg ctggggtcaa gctgcaatac tgccgaaccg agcgattcgt agcggttgag
     1501 aaggtaaaac gggacggagt tgagcgatgc gtagagtttc agaaagctca aaccggattt
     1561 gtgggcgatg gtttccgcct gatgaagcag ggcagtgccc agtccgaggt tgtggaacag
     1621 ggggtggacg taaagtgcat cgagttgtgc ttcttggcag tcgatttgga aaaatccctg
     1681 tatgttgcct ttgtattcgg caacccaaag tgctttgtcg ggatcggaaa tggtcggcag
     1741 gtagctttct gtgtttaaca agccttccca tacttttagg gcgtgttcgt tgtagctgag
     1801 gatgcaggtg tattggacgg agtgcaggtg gactttgaag atgtctttgc agtcttgcac
     1861 ggtggcgggg cgtaacagtg tcagcaggtt catggcagta tgtcggcggc ttcagacggc
     1921 atctgtgccg ttggtcggat tatagggact gatgcagttt ttttgcttct tgaaatgcgg
     1981 tgtccgaatc ggtgcttaaa atggtaaagt gtcccatttt ccgccctttg tgcgcggttt
     2041 ttttgccgta aaggtgcagg tgcgcgtccg gccggctttg caggggaaac caatccggtt
     2101 cgccgccgtc ttcctgccaa acgtcgccca aaatattcgc catacagcaa gaactcagca
     2161 atttggtgtc agcaggtggc aggttgcaca tcaggcggac ctgttgctgg aattggtctg
     2221 ccgcgcaggc gtcgacggta tggtggccgg aattgtgcgg acgcggcgcg atttcgttga
     2281 cgaccaattc atgcgtgtcg ccgacaacaa acatttctac cgccaatacg ccgacgtagt
     2341 tcaattcatc ggccaaacgc tgcgccattt gtcgcgcctg ttgctgaatg tcggcactca
     2401 gtcgggctgg gacgatggag taggcgagga taccgttttc gtgaatgttt tcggcaggat
     2461 cgaaagtttg cacgttgtca ttgttcagac ggcatacgat aacggaaatt tcgccgcgca
     2521 agtccaccat tttttccaaa acgcaatcca cgccgcggtg ttcggcaaac gcggctttga
     2581 gttcatccac cgttttgacg cggatttggc ctttgccgtc atagcccaat gtagcggttt
     2641 tcaggatgcc gggcagaaat tgtatgcttt cttcagtgat gtcttcggct ttgcaaattg
     2701 cttgatacgg cgcggtttgc aggcctgctt tgcgtatcca tgccttttcc tgaatgcggt
     2761 tttgcgcgat ggcaacgcag tcgccgctgg gggaaacatt ggtatgtttg gcgagaaaac
     2821 gcatcgcatc ggcattgacg ttttcgaact cggtcgtaac agccgcacat tttgccaatt
     2881 cttccaaagc ggtttggttg tcaaacggcg cacacaaatg gcggtcggca aattccgctg
     2941 ccggcgcatt cgggttggga tcgagtacgg ttactttgta gcccatggtt ttggcagcaa
     3001 cagtaaacat tctgcctaat tgtccgccgc caagaatgcc gagcatggcg ggcggagaaa
     3061 gagatatgtt tttcatgctg actcttcaaa ttgtacaagt tgatagctat aactaattct
     3121 tgacggatgt cttgtatcgc tggaattacc agtttcagaa atacagaata ctttttccat
     3181 aaatttttct gcttttagaa attccagtat tctgtttttt tcatccttat aagcactgcg
     3241 gtctgtaccc catgcaagaa taatcatatc agcatcttcc aaacatcctt taaatttgga
     3301 aaaatcggtt tgggtgtttg ccctaattcc tgtttgctgt gtagagtaac tggaaaaaat
     3361 atttaacatt ttgaagttgg taaaaccgta catatccaag aaacgtgcaa gttgggtaag
     3421 ggttttgtcg cttctttcat catttgcttt acttggatta attcctatag caacagctga
     3481 gaaattttta ggattttctg tgttgccgct ccatctaacc gttaggattt ctcgactttt
     3541 ttcattatct gtatagagac cgtctttggt agtcggtctg agtgtttggc gaagctcata
     3601 aagtttttca taagtcattt atccaaccct tcctgcacca tctgcgcggc gcgtataacg
     3661 gctcgggctt tgttttgtgt ttcctgccat tcggaggccg gatcggagtc ggcaaccacg
     3721 cccgcgccgc tttggacgta tagcgtgttg ttttttacta cggcggtgcg gatggcgatt
     3781 gccaaatcca tgtcgttgtt gaaaccccat acgccgacgg caccgccgta gatgccgcgt
     3841 ttgctcggtt cgacttcttc gatgatttcc atggcgcgga ctttgggtgc gccggagagt
     3901 gtgccggcag ggaaggtagc ggcgaggatg tccatgttgg tcatgccgtc tttcagacgg
     3961 ccttcgacgt tggaaacgat gtgcattaca tgggagtatt tttcaatcac cattttgtcg
     4021 gtaactttga cttcgccggt tttactgatg cggccgacgt cgttgcgtcc taagtcaatc
     4081 aacatgacgt gttcggcgat ttctttggca tcgcttaaca aatcttgttc gttggcaagg
     4141 tcttcggcgg gggttttgcc gcgcaggcgc gtgccggcga tggggcggac gatgacatcg
     4201 tcgcgttcgc ggcggacgag gatttcgggc gaggagccga cgatgtggaa atcgccgaaa
     4261 tcgtagtaaa agagataagg cgaagggttg agcgtacgca gggcgcggta gagggcgagc
     4321 gggctgtcgg tgaattccat gctcatgcgc tggctgggga caacctgcat gcagtcgcct
     4381 gcgaagatgt agtctttgat tttgttaacg caggctttga acggctcttc gccgaattcg
     4441 ctgacggctt cggtgtgttt gctgccgagc gagagcggga tggcgcagct ttggcgcaac
     4501 tgggtgcgga tgtcttcgag gcgttcgcgg gcgcgttcgt agccgtcggg ctgcgacgga
     4561 tcggcgtaaa cgacgagatg gattttgccg ctcaaattgt cgatcaccgc caattcttgc
     4621 gacagcatca gcaagatgtc gggcgtgccg agcgggtcgg ctttggtggt gtttttcagg
     4681 cggtgggcga agtgttcaaa attgtagatg gtttcgtaac cgaagtagcc gaccagtccg
     4741 ccggtaaagc gcggcaggct tgggatttcg ggtgttttga agcggttgtg gaaggcttcg
     4801 ataaagggca gggggttgcc gtcgtgttgc tcgacaattt cgccgttttg ataaacatcg
     4861 acgtgtttgc cgctggcttt gagatagtgg ctgcaaggca ggccgataaa agaatagcgg
     4921 ccgaaacgtt cgccaccgac aacggattcg agcaggtagg tataggggcg gttggcgagt
     4981 ttgagataga gggaaagcgg cgtatccaaa tcggcaagga gttcttgcac gagcgggatg
     5041 cggttgtagc cttgggcggc ttgggcttgg tattcttgtt tgctgatcat ttctgctttc
     5101 ccaaagggcg gtttcggacg gcgcggcaac gggcgcgagt atagcatttt atcggaattg
     5161 ttgacagtct gaccggagat gccgtctgaa aaggacgggc ttacggcttt gttttgcgct
     5221 gccggtattg tttccattta aacagataac ctgcggttac gggaatcagg gctaagatgg
     5281 ttttgaacgt ccatgatgca taccaaggta aagaaccggg tgcgaccgat tgattcagat
     5341 acggccacca acaggcagtc aggcagccga gcagcagcgg ccagatatgc cggctgaggt
     5401 ttttttgcca aaggaggaat gatgcggcag accaaagcag ggtaagggca gtgattttta
     5461 acacggtata agcatccgct ccaaacaggg cggcgagaaa atacgtaccg gcaatccaga
     5521 ggacggcaag tatcaggaca atgatttctt caggtcgttt gaacattttt ttcttcctgt
     5581 ttgatttcag acggcattgc cgttctgttt ggtttccagc agctcccagc gttccagctt
     5641 ttccaaaagc agcatttcga tttcttcggc gcggctttgc agtgtgccgg ctttttcgta
     5701 atctttgaag atttcgggat cggaaagctg cgcgttgatt tctgcctgtt cggcttccaa
     5761 ggcggcgatt tcgtcgggca gggcgtcgag ttcgcgctgt tctttgtagg acagtttgac
     5821 cgtgcggttg gctttgggtt tttctttcac cggttcggca tcggctgttt tgggtgcgga
     5881 ttccgtctga atcttcgctt cgcgtgattt tgcgtcgatg tagtcctgat agccgccgat
     5941 atattctttt aaccttccat ttccttcaaa aataatgctt tgggtaatca cgttatcgag
     6001 gaacatacgg tcgtgtgaga caaggaatac cgtgccttga taatcgcgca gcaaatcttc
     6061 aagcagctct tgggtatcga tatccaagtc gttggtcggt tcgtccaaaa tcaaaatatt
     6121 agcaggacgg gtaaagagtt ttgccagcaa aaggcggttg cgttcgccgc cggagagcga
     6181 tgaaacggga ctttgtgcgc gggctggatg gaataagaaa tcttccagat agctcattac
     6241 gtgttttttc ttgccgccga cttcgacgta atcgttgcct tgaccgagcg tgtaaaacac
     6301 ggtgtcgttt tcgttcaacg tgctgcggaa ttggtcgaaa taggcaactt cctgcttact
     6361 gccgatacgg attctgccgt aggtcggctg caattcgccc aaaatcagct tgaggaaggt
     6421 ggttttgccg ataccgtttg ggccgattaa gccgattttg tcgccacgct gcaagatggc
     6481 ggagaatttg tccatgatga ctttgtcgcc ataggtaaac gaggcatgtt ccagttcggc
     6541 aatgattttg ccgcttttct cgccgctgtc gagcttgaaa ttgacctgcc cttgtacgtt
     6601 gcggcgttcg gcacgctggc ggcgcagttc ttccaaacgg cgcacgcggc cttcgttgcg
     6661 ggtacggcgc gcttcgatgc ctttgcgtat ccacgcttct tcctgcgcgt ggaatttgtc
     6721 gaagaggcgg ttgtgttccg cttcgactgc caactcttgc gcttttttct cgctatattt
     6781 ggagaacgag ccgggatagg aacgcaaaat accgcgatcg agttcgacaa tccgcgtggc
     6841 gatattgtcc aaaaaacggc ggtcgtgggt aatcacaacc aggctgcctt caaacgcttt
     6901 gagcaggttt tccaaccaaa taatcgcgtc gatgtccaaa tggttggtcg gttcatcgag
     6961 cagcaatacg tcgggcttct gcacccaagc ctgcgccaag gcgacgcgct ttttctgacc
     7021 gccggagagg ttgccgattt tttcgttttc cggcaaaccg agttcgccca aagtctgctt
     7081 caccgccgca tccaacttcc agccgtcctt cgcttcgatc tcaagttgca attcgttgag
     7141 ctctttcaat aaggcctcac tcgaaccatt ttccaactca tggctgacat gatgataacg
     7201 gcgcaataaa tcgcgaattt cgcccaaacc ttcggcaaca gtatcaaata cggttgcttc
     7261 cttatcaaaa aaggattcct gcggtacata aacgattttg aggttgtttt ggacaataat
     7321 ctgcccatca tcaagctttt gcacgccggc gaggattttt aaaaacgaag acttacccgc
     7381 accattacga ccgattaagc cgattttttc gccgctgtcg agttggaagg aagttttgtc
     7441 gagcagggcg acgtggccga cggcaaagga agcgttttct acagataata tattcatgat
     7501 acaaattctc aacagttacc gtttggattt taccgcaagt ttggcgcggg caatttcaac
     7561 cgcacccggc aggacggaaa caataatgat gccgcccatc accaagccca gattgttttt
     7621 tacgacgggg aagttggcaa agaaatagcc cgcgtaagaa aacaggataa cccacaacaa
     7681 gccaccgatg atgttgtagc ggataaattt ggcatagtgc attttcccca taccggcgac
     7741 gaagggggcg aaggtgcgga cgatgggcat aaaacgggca atgatgatgg ttttgccgcc
     7801 gtgtttttcg taaaaacggt gggttttatc gagatattca cgtcggaaga ttttagaatc
     7861 gggattggcg aacagcctgc cgccgaaata tttgccgacg gtaaaattga gcgcgtcgcc
     7921 gagtatggcg gcaaggctta ataatgcaac catcaaatga atatccatac cgcccagcgc
     7981 ggcaataccg ccggcggcaa acagcagcga atcgccgggc agtaagggcg taacaatcag
     8041 gccggtttcg caaaaaacaa tcaaaaacag aatcgcataa atccacacac cgtattgcgc
     8101 cgacagcgcg agcaggtgtt ggtcgatatg gaggatgaag tcgatggcgg aggcaagcac
     8161 ggcgcgttcc taaaaaaaac aaaccgcgta ttttaaccga ttggaaaaat gccgtctgaa
     8221 attttcagac ggcatcggct attcaaattc atttcacgta aaaaccgcaa accaaaatag
     8281 tttgcggttt ggcatttaaa gtgacaatga tgatttcaaa tcatcagaat tttatgccga
     8341 cgcgcaagcc gtattcacga atactggttt tcgggatggt gagcgatacg tcgccactct
     8401 tggttgttac actaaactcg ccggattctt tgtaagtgcg ttgtttgtag aacggccccg
     8461 cctcgatgct ggcggattcg cccagttttt taccaatatt tgcacccaat ccaaagcccc
     8521 agccgttggt tttttgattg atgtcttttt tgagattggt aacgccggtg tttaatttat
     8581 agcgggaatt gaggtcaaat ttcacttcag accaagggtt gatataccag ccgttaccca
     8641 gttgggaaag caaatccgcg tgaactttgg ctaaccacga ctgacggctg ctgtgaagcg
     8701 tatgcttggt ggttttaatg ctgtcttttg aagattcaaa acccaagccg gcacccacac
     8761 ggaaatttaa agaatcactt aacgtttggg tgtaggtgta gcctgtgtaa agatcgatac
     8821 ggttttcagg aacgccggtg ggcagtttta catttttagt cttacccagc ttgttctcat
     8881 ctgtttccaa attaataata ttttttttgc tgcgcccgaa accggcttcc aagcggatgc
     8941 cttggttggc atcaaaagga atatcagcac gtacgccgat gtgtttggca gctttgtgtt
     9001 tttctttcag gaaagcacga gttgaagaaa tggaagagag gtcggtgtgg acggtaaact
     9061 cattagcggt ttgaagctct tgtgcagcgg cggcagtacc ggtcagggca atcatggcac
     9121 atgtaaaaac tgtttttttc atagttaaaa tctccaaaat ttggattgta gtcggatatg
     9181 gtaacataac gtaaataatc gttacgctta caattatatt cttaagcttt cggggggggg
     9241 ggattttaca tatattaata aaaattaaca aatagttatt tgtttacaac gaattgttat
     9301 tctcacttgg ttttctgttt tttatgggaa tgacgaaatt ttaggtttct gatttcggtt
     9361 ttctgtttta agggaatgac gagacttgag atggcggcat ttatcgggag caactgaaac
     9421 cactctgccg tcattcccgc gaaagcggga atctaggttc gtccggtttc ggttatttcc
     9481 gatagattcc tgccgcgttg ggggtctgga ttcccgcctg cgcgggaatg acgaatttca
     9541 ggttgctgtt tttggttttc tgtttttgtg aaaataacag gatgaga
//
LOCUS       DAWKQB010000072         9507 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.072, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000072 DAWKQB010000000
VERSION     DAWKQB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.072"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(74..1495)
                     /locus_tag="WJL14_001292"
     CDS             complement(74..1495)
                     /locus_tag="WJL14_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ7656357.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1853..2212)
                     /locus_tag="WJL14_001293"
     CDS             complement(1853..2212)
                     /locus_tag="WJL14_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ7656358.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2209..2814)
                     /locus_tag="WJL14_001294"
     CDS             complement(2209..2814)
                     /locus_tag="WJL14_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ7656359.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIIQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2861..3343)
                     /locus_tag="WJL14_001295"
     CDS             complement(2861..3343)
                     /locus_tag="WJL14_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ7656360.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKQTTSLPLNHLLKE
                     "
     regulatory      3547..3644
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3855..4955
                     /gene="gcvT"
                     /locus_tag="WJL14_001296"
     CDS             3855..4955
                     /gene="gcvT"
                     /locus_tag="WJL14_001296"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ7656361.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5118..5504
                     /gene="gcvH"
                     /locus_tag="WJL14_001297"
     CDS             5118..5504
                     /gene="gcvH"
                     /locus_tag="WJL14_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ7656362.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPGAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5656..6903
                     /locus_tag="WJL14_001298"
     CDS             5656..6903
                     /locus_tag="WJL14_001298"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ7656363.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            7179..7604
                     /locus_tag="WJL14_001299"
                     /pseudo
     CDS             7179..7604
                     /locus_tag="WJL14_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            7610..8839
                     /locus_tag="WJL14_001300"
                     /pseudo
     CDS             7610..8839
                     /locus_tag="WJL14_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            8897..>9507
                     /locus_tag="WJL14_001301"
     CDS             8897..>9507
                     /locus_tag="WJL14_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656364.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRI"
BASE COUNT         2421 a         2361 c         2624 g         2101 t
ORIGIN      
        1 gtcggaaggg gggatattga tttgattatg ccggaattta aaatgccgtc tgaatgttca
       61 gacggcatag cgtttacagc agtttgaaaa cgaaaaagat aagggtatgt acgatgaaga
      121 cgggtgtcag gaaggcgacc gaccacatca tatagccgaa gaaagtcggc atcggtacgc
      181 cgcgctgttc ggcaatggcc ttgaccatga agttcggtgc gttgccgatg taggtcagtg
      241 cgcccatgaa taccgaaccc atagaaaccg ccagcagcga atgaaacagg gaacccgtca
      301 tcaaggcttg ggcatcgccg cccgccatat tgaaaaaaac gagataagtg ggcgcgttat
      361 ccaagaatgc cgacaatatg ccgctcatcc aaaaatacat cacattaatc ggatgacctg
      421 ccgtatcgtg aaccagcgat accaccccgc ccagcgcgcc tgcctcgcct gctttcagaa
      481 tgctcaggac gggaaagatg gtgatgaaga tgccgaggaa gagtttgccc acttcggcga
      541 tgggttcaaa gttgaattcg ttgcctgcgc ggacttgttt gggcgtgatt gccatagata
      601 cggcggtcaa tgcaatcagg atgacatcgc ggacgaggtt ttgcagggcg taacggctgc
      661 cgaggatttc aaatcccggg tgttcgggtt tccaaaggcc ggacattaga accgcgccga
      721 ccacgcccga aagcaggagg aagttccatt tgccgaagat ggcgattttt tcgggttttt
      781 cctgttgtgc cggcgtatct tgtgcaatgc tttcctgttt gaagaaacgg ttgtcgatga
      841 aatagaaggc ggtcaacagg acagcggtgc tgatcaggac gggggcgaac atatgtttga
      901 ccgtccacat gaaatctacg cctttgagga agccgaggaa gagtgggggg tcgcccaaag
      961 gggtcagacc gccgccgatg tttgcaacca ggaaaatgaa gaagatgacg atgtgcacgc
     1021 ggcgggtacg gttttggttg gctttcagca gcggacgaat catcagcatt gctgcgccgg
     1081 tcgttcccat gatagaggca agtgccgtac cgacggcaag cagggcggtg ttgagcttgg
     1141 gtgtgccgtt caagtcgccc caaaccaaaa tgccgcctga aatggtgtac agggcaagca
     1201 gcagcaggat gaaagggatg tattcttcaa cgagtgcgtg tgcgacggta tggataccgg
     1261 cggacgcgcc aaaaaccaaa ctgaacggga tgaggaagag caatgtccaa aaggcggtaa
     1321 ttttgccgta atggtgatgc caggtatgcg aaaaaaacaa gggacccaat gcgatagaca
     1381 gcaaaatcag ggcaaagggc aggctccaca gcaggtttag gtttgcgccg tccaaatctg
     1441 cggcgtaaac cgatgcgggg aaaagcatta gtgaaaacag gggtaggtgg cgcatcgtgt
     1501 ttcctcgatt caagcactgc cttgcgcggc gcgtgggagt gatacaggca ccgtgccgcc
     1561 cggacatagg cggactgtgc tgcctgtttg tttttgaaaa aagttttccg attgattgta
     1621 aagtaaataa tcaggataaa ccagtttccc aaaccggaag gcggcgggaa ggcggattgc
     1681 tgtgcttggg aatatatgtt ctttttgata aataatttta tttaaaacaa ataaatatat
     1741 gtctttaata aataatctat cgaaaacgaa aaatgaattt attttaacat atatttgcaa
     1801 tgaaacaggt ttgccccccg tttgtttgcc cttatccctt tcagtacggc attcaagatt
     1861 cgggcctgcg ccacatccat atggcgacaa gggaacaaaa aaccgatgaa accgccccga
     1921 cccaccagcg ttggggaaac tgccaaaaca ttatcaggca ggatgcggtc atcatactga
     1981 tggcgaatat tttggctttg cgcggcactg cgccgttttg ttcccagtta tgaaccatcg
     2041 gaccgaaata gcggtgccgg tgcagccagc ggtgaaagcg cggggatgcc tttgcccagc
     2101 aggcggcgga gagcagtacg aacggcgtgg tcggcaacag cggcaaaaaa atgccgatga
     2161 tacccaacag cagggaaatg cagccgcagg caattaaaag ataacgtatc attttgaaat
     2221 atttttctta ttgtgcggat aagggcagga tgtgataccg agttttgccc agccctcatg
     2281 tcccattttt tccagcaggg cgatattgcg ttcgaatatt gccgaggcgt cgggaaaggc
     2341 ttgtgcggct ttggcaatgc tgtcttcgcg gatgaggtgc agcgtcggat agggagaacg
     2401 gttggtgtag ttgccgatgt cgtctgaatc cgtgccttca aattggaaat cgggatgaaa
     2461 cggggcgatt tggatgatgc cttccaagcc gttttcgaca acggcggcat cggcaatgtc
     2521 gagcatatcg ttgaatacgt cgaaatcggg gaatagggtc gggtgaacca gcagggtagt
     2581 ttccagttcg gtggcgggtg tattgcccag tcgctgcagt tcttcgtcca agtcttccaa
     2641 aaaactgtca aggtgtttgg cttcgctgat tgcgatgcgg acaaggtttt taacgtgggg
     2701 ggctttggca aagggacaca ggttcagacc gatgacggct ttttccaacc attgtccggt
     2761 gtgttcggca acagcatctt tattttcgga agtattgata ttcattattg tcatgtaaat
     2821 gtgtttgcag attgcacgtg cgggaaaatc gggaagggca ctattccttc agcaggtggt
     2881 tgagcggcag ggaggtggtt tgtttgattt cttttaaaac aaagctcgat tgtgcatctt
     2941 gtacgccgtg gtgggacagg agcgtatcca aaacaaaatg ggaaaacgcg ttcatatcgg
     3001 taaaaaacgc ctgaagcagg tagtcggttt cccctgtcag ggcgaagcag ctcaagactt
     3061 ccggccattt tcggaccgat gcggcaaagt cttcccgcgc gtcttttgct ttgcggatgg
     3121 aaacgcggat aaatgcctga agccccaggt tgacggattc cggagacagc agcgcggcat
     3181 attggcggac gatgccggca tcttccaact gctttagacg gcgcaggcac ggagaaggcg
     3241 aaagtgcgac acgttcggac agttcgacat tggtcagcct gccgttttcc tggagaacct
     3301 gtaagatttt aatatcggtt ttgtctaaag tgagttgggg catatttgcg ttccgtttta
     3361 aggaattcgg attgtctgtc cgtatgtttg cggcaatccg cacagatgga gaccatatta
     3421 acatataaaa agttataccg tcatccggga caaattttgt tttcggaaaa tcatgtgaaa
     3481 acagaggcgg tcggtttgca tctcttttaa gacggcttgc ccaaaccgcc gattcaagac
     3541 ataatcggga aatgtgcagg agagtgttac acccaactac aatgtaacca ccgaaggcgc
     3601 agacaccctt aaatcgctca ggtatcaggg actgcacatt gaaacaaaca atctggagag
     3661 cggcgttgga ataacgtcca ccgaagggga gaaggccgtc tgaaccacca ttcagacaac
     3721 cgcgcaaagc agtgagcaga ctggtttgcc atcatgcgga tacggccgaa aatctcaggt
     3781 tcaaggacag atagggtcat ccgcgcacag gtgcgcgggt ggcatctgaa caaaaaatcc
     3841 ggagaaactt gagaatgatt actctgaaaa ccaccccatt tcatcaagcc catcaagatg
     3901 caggcgcgaa gctggtagat tttgccggct gggagctgcc catccattat ggttcgcaaa
     3961 tcgccgaaca cgaagccgtg cgcaccgacg caggtatgtt tgacgtatcc catatgcttg
     4021 ttaccgacgt agcaggcgca aatgccaaag cctttttccg caaattgatt gccaacgatg
     4081 tcgccaagct cgcttttgtc ggcaaagccc tttattccgc tttgctcaac gacaacggcg
     4141 gcgtgattga cgacttgatc gtttaccgca ccaatgaagc cgaaacccaa taccgcatcg
     4201 tgtccaacgg cgcgactcgc gaaaaagata cggcacaatt ccacaaagtc ggacaagagt
     4261 tcggtgtttc cttcaatccg cgctacgacc tcggcatgct cgccgtacaa ggccctaaag
     4321 ccattgaaaa actcctgacc gtcaaacccg aatgggcaga tgtcgtccat aacctcaaac
     4381 cgttccaagg cgcggatttg ggcaacgact ggtttgtcgc ccgcaccggc tacaccggcg
     4441 aagacggcgt cgaagtcatc ctgcccggca ccgaagccgt cgcattcttc aaagccctgc
     4501 aagcagcagg cgtacaacct tgcggtctcg gcgcgcgcga caccctgcgc atggaagccg
     4561 gcatgaacct ctacggcaac gatatggacg acgataccag cccgctcgaa gcaggcatgg
     4621 gttggaccgt tgatttgaaa gacgaaagcc gcgattttgt cggcaaagct gccttgttgg
     4681 cattgaaaga aaaaggcgtt gccgtcaaac aggtcggttt gctgctcggc aaaggcggca
     4741 tcctgcgcgc gcatatggaa gtgttgaccg acaaaggcaa aggcgaaacc accagcggcg
     4801 tattctctcc aagtctgaaa caatccatcg ccatcgcccg cgtaccgaaa gattttgacg
     4861 gcgataccgc caaagtgctg attcgcggca aggaagcgga cgtgcgcgta ctgaagctgc
     4921 cgttcgtccg caacggtcaa aaacagtttg attaatatcg gttcagacgg cattttcatt
     4981 tcatatgccg tctgaaagca ggttttaatt gtcgtccgat acggacgttt gtagaaagca
     5041 ttgaacaagg catctgtgga tattgattca tgcagatgcc gtctgaaaat aaccccctat
     5101 caatggagta tcaaaccatg agcagcaaca tcccggcaga actgaaatac gttgccagcc
     5161 acgaatggct gcgccttgaa gaagacggta tcatcaccgt cggtattacc caccacgcgc
     5221 aagagctgtt gggcgacatc gtgtttgtcg agctgcctga agtcggcgca aaccttgccg
     5281 ccgaagaaca agccggtgtg gttgagtctg taaaagccgc gtccgacgtg tacgcaccga
     5341 ttgcaggtga agtcgttgcc gtcaacgaag atttgccagg cgctccggaa actgccaaca
     5401 gcgatcctta cggtgcaggc tggttcttca aactcaaacc ggcaaacgtt gccgattacg
     5461 acgttctgct gactgccgaa caatacgcgg gcgaagtgga ttaaaccgcc cggctgcccg
     5521 atggcaaccg ccggacaaac ggaaactgca ccttcagacg gcatttttgc ggtcggaggt
     5581 gcagtttttt gtccgtgttt taaggaagca gttaggctat aataacggtc tatattcatc
     5641 tttaccgatt ttttcatgca acttaccgct gtcggactca atcatcaaac cgcaccttta
     5701 agcatacggg aaaagctggc gtttgccgcg gcctgcctgc ccgaagccgt ccgcaatctt
     5761 gcccgaagca atgcggcaac ggaggcggta atcctttcta cctgcaaccg taccgagctt
     5821 tactgtgtag gtgattcgga agaaatcatc cgttggctcg cagactatca cagccttccc
     5881 atagaagaaa tcagccccta cctttatact ttggggatgc aggagactgt gcgccatgct
     5941 ttccgcgtcg cctgcggctt ggattcgatg gtgttgggcg agccgcagat tttaggacag
     6001 attaaggatg cggtcagggt tgctcaagag caggaaagta tgggtaagaa actcaatgcc
     6061 ctgttccaaa aaaccttttc tgttgctaaa gaggtccgta ccgatactgc cgtcggcgaa
     6121 aactcggttt ccatggcttc cgcttccgtc aagttggcag agcagatttt ccccgacatc
     6181 ggcgatttga atgtcttgtt tatcggtgcg ggtgagatga ttgagctggt tgccacttat
     6241 tttgccgcca aaagtccccg gctgatgacg gttgccaacc ggacgctggc gcgtgcacag
     6301 gagttgtgcg acaagctcgg tgtcaacgcc gaaccgtgcc tgctgtccga tctgcctgcc
     6361 attttgcatg agtacgacgt ggtggtttct tcaacggcaa gccagttgcc cattgtcggc
     6421 aaaggtatgg tggagcgcgc attgaaacaa aggcagagta tgccgttgtt tatgcttgac
     6481 ttggccgtgc cgcgagacat tgaggcggaa gtcggagatt tgaacgatgc ctatctttat
     6541 acggtggacg atatggtcaa tatcgtccaa agcggcaagg aggcaaggca gaaggccgcc
     6601 gccgccgccg aaacgctggt gtccgagaag gttgccgaat ttgtcaggca gcagcagggc
     6661 aggcagagtg tcccgttaat cagggcattg agggatgagg gagagaaagc gcgcaaacag
     6721 gtcttggaaa atgcgatgaa acagcttgcc aaaggcgcaa cggcagaaga ggttttggaa
     6781 aggctgtcga tccaactgac caacaagctg ctgcattcgc cgacccaaac cttgaataag
     6841 gcgggggaag aagataaaga tttggttcac gccgtcgcgc agatttatca tttggacaaa
     6901 taacggtgcg ccggaaaagg atgccgtctg aaggggtttc agacggcatt ttttgtgcag
     6961 gccggcaaca tcgtgaaatc ccacattata tcgatgtaat cacaaagtat agtggattaa
     7021 caaaaatcag gacaaggcga caaagccgca gacagtacag atagtacggc aaggcgaggc
     7081 aacgctgtac tggtttaaat ttaatccact atattatccc gtatgcggat tggttttaag
     7141 atttgtaaat ttgatttgca tcaaaaaatc gccgatagat gattcatata atatcaatat
     7201 taaagagtat cggtatatcg gggatagtca tgtcctgttt ttcaatcaaa cgtatgtccg
     7261 cgtttcgggc gcggataacg gcgttttttg ccgcctttgt ctttttgacg gcggcactgc
     7321 ccgcttatgc ggagcgtctg cctgattttc tggcgaaaat acagccttcg gaaattgttc
     7381 cgggtgcgga ccgttacagc aagccggaag gtaagcctat ggttgcccgc gtttacaaag
     7441 gcgatgagca gttgggcttg gtctatatca cgaccgatgc ggtcaatacg cgcggttatt
     7501 cgagcaaacc gattgatacg ctgatggcgt tggctaaaga cggtacgata gccggagcga
     7561 aattggttga tcaccatgag tcgattatgc tgatcggtat cccgcatttg cccgcgcccg
     7621 ggcgggcgat acgctcaaac tggcttccgg cgtatatcaa accaaacttc acatcgacaa
     7681 accgattacg attgaagggc ctgccgaccg ttctgcaacc atcgaaggag acaggagcgg
     7741 gcgtaccata gccgtacacg cgccggacgt aacgctccgc aacctgaccg ttacccgttc
     7801 cggtatgagc ctgcccgcaa tggatgccgg tatttatctc gaagaaactg ccccgcgcgc
     7861 cctgattgaa cacaataata ttttggataa ttcggtcggc gtctatctgc atggttctgc
     7921 cgatgcgatg gtgcgggaga ataaaatcgt cggcgacgcg actttgcgcg tgaacgagcg
     7981 cggcaatggc gttaccgttt ggaacgcgcc cggcgcgcag gtcgtcggca acgatatttc
     8041 caaagggcgg gacggcattt tttccaatac cagcacgcac aacacctata aaaacaaccg
     8101 cttcagcgat ttgcgtttcg ccgtccacta tatgtacacc aacgacagcg aaatcagcgg
     8161 caatatttcc gtgggcaaca atatgggcta tgtgctgatg ttttccgagc ggctcaaagt
     8221 gtttgacaat atcgccgtcg gcagccgcga ccaaggcatc atgctcaact atgtcaacta
     8281 ttccgatatt cacgacaaca ttatcaacaa agcgggcaag tgcgtttttg cctacaatgc
     8341 caactacgat aaactgtccg ccaatcattt tgaaaactgc caaatcggca tacactttac
     8401 cgccgccatc gaaggcacgt ccctgcacga caattccttt atcaacaacg aaagccaggt
     8461 caaatacgtc agcacgcgct ttctcgactg gagcgagggc ggacacggca actattggag
     8521 cgacaacagc gcgttcgatt tgaacggcga cggcttcgga gacagcgcgt accgtcccaa
     8581 cggcatcatc gaccaaatca tctggcgcgc acccgtatcg cgcctcttga tgaacagtcc
     8641 cgcaatcagc atcgtcaaat gggcgcaggc gcaatttccc gccgttttgc ctggcggcgt
     8701 ggtggacagc aaaccgctga tgaagcctta tgcccccaaa attcaaaccc gttatcaggc
     8761 gatgaaggac gggctgctca aagaagtcga aacgcggcag ttggaatggg gcagggcgga
     8821 aaacggttct ttgaactagt ctgcttcaga cggcatccgg attcaaatgc cgtctgaaaa
     8881 cacaaaagga acaaccatga ccacacatca tgtcgaattg aggaaggtaa ccaaacgctt
     8941 cggcgcgcaa aaagccgtca accaagtcga tttggttttg aaggccggag aaagcgtcgg
     9001 acttgccgga cacaacggtg cgggcaagtc caccattatg aagctgatac tcggtctgat
     9061 taccccgacc gaaggcgaag tgatgctttt gggcgaacgt accggcagca aagcgggggc
     9121 gcggcttcgc agccaaatcg gctacctgcc cgaaaccgtt gcgttgcacc cttcgctgac
     9181 cggcatcgaa acgctggact tttacgccaa acttaaaaaa cagccgctca cccagaacag
     9241 ggggctgctc gagcgcgtcg gcatttcaca ggcggcacac cgccgcgtcg gcacctactc
     9301 gaaagggatg cgccaacgcc ttgccttggc acaagccctg ctgggcgagc ccaaagtcct
     9361 gctgtttgac gaaccgacaa ccggcctcga ccctgcatca aggcaaatgt tttacgaagt
     9421 cgtgcgcgaa ctcaacgggc gcggcgcgac cgtattgctc agcacccacg cccttgccga
     9481 gttggacgga cacgccgacc gcattat
//
LOCUS       DAWKQB010000073         9501 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.073, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000073 DAWKQB010000000
VERSION     DAWKQB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.073"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(138..1055)
                     /gene="thrB"
                     /locus_tag="WJL14_001302"
     CDS             complement(138..1055)
                     /gene="thrB"
                     /locus_tag="WJL14_001302"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ7656365.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1092..1820)
                     /gene="ubiG"
                     /locus_tag="WJL14_001303"
     CDS             complement(1092..1820)
                     /gene="ubiG"
                     /locus_tag="WJL14_001303"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ7656366.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVVETKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1972..3213
                     /locus_tag="WJL14_001304"
     CDS             1972..3213
                     /locus_tag="WJL14_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ7656367.1"
                     /translation="MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFAGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3363..4417
                     /locus_tag="WJL14_001305"
                     /pseudo
     CDS             3363..4417
                     /locus_tag="WJL14_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(4484..6136)
                     /locus_tag="WJL14_001306"
     CDS             complement(4484..6136)
                     /locus_tag="WJL14_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ7656368.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLDVPWRWAGAVLFAAAFVSMRHFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFARQMPSETRPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGFVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGSIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRKLVGDYRNYLYDVLKIAS"
     gene            6306..6877
                     /locus_tag="WJL14_001307"
                     /pseudo
     CDS             6306..6877
                     /locus_tag="WJL14_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            6874..7437
                     /gene="gmhB"
                     /locus_tag="WJL14_001308"
     CDS             6874..7437
                     /gene="gmhB"
                     /locus_tag="WJL14_001308"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ7656369.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            7466..8209
                     /locus_tag="WJL14_001309"
     CDS             7466..8209
                     /locus_tag="WJL14_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7656370.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            8206..8898
                     /locus_tag="WJL14_001310"
     CDS             8206..8898
                     /locus_tag="WJL14_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7656371.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            9009..9194
                     /locus_tag="WJL14_001311"
     CDS             9009..9194
                     /locus_tag="WJL14_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656372.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         2374 a         2948 c         2218 g         1961 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tataaaatgg aaaatacccg gctatcgtct
       61 cattttcgtt ttaatcagcc ataaaaatgc cgtctgaaac cctttcagac ggcatttctg
      121 tcaaacgccg gacgcactca atccaaactc aacagcaggt tgcggaacgc gttcgggtct
      181 ttgataaacg tcatctcgcc cgcctgcgga aaatgaaagt ccaacaggcg cgacacccaa
      241 aaacggatgc agccggcacg ttgggcggtc gggaaatacg ccttttcttc ggcactcaag
      301 gggcgcacgc cctcataacc gccgataaac gcctttttca acgcctcatc caacttattg
      361 tccgctgtcc ttgcccaatc gttgaccgca atcgccaagt catacataaa attgccccgg
      421 caggcataat agaaatcgat gaaacccgat acctgaccgc cgtcaagcaa cacattgtct
      481 ttaaacaaat cggcgtggat gatgcccgaa ggcagatgat tgccgagatt gtccttcagc
      541 gcatcgattt cggaacacag cagcgcggca tcgtcttgcg acaggacggg cagcagccgg
      601 gtgcacgcct ccgtccacca cgcatcgtaa cgcgggtttt ccatttccaa agggaaatcg
      661 gcggcggcaa ggtgcatttt ggccagcatc gcaccggtat ggaaacactg ctccgccgtc
      721 ggcagcgcgg tatccgaacc tttcaggcag gcaaccaaac aagccggctt gcccgccaaa
      781 acggaatcaa gccggccgtc tttgcgcgca accggcgcgg caaccgccac gcccttcata
      841 ctcaaatgcc ggttaagctc cagaaaaaac ggcagctctt cctgtttcaa cacttcaaac
      901 acggtcagca cataacgtcc cgaagtcgtc gtcagaaaat aattgctgtt ggtaatcccc
      961 tgcgcgatgc cctgcaggga aacaaattcc cccaaatcgt aaccgctcag gaagccgcgc
     1021 atttcatcat cggaaacact ggtatagaca gacatatcaa ctcacttgct caaaaacggg
     1081 caaacccgcc gtcagaacgc cggacggcag gcaaacatat aattcacatc ggtcgaatcg
     1141 cacagggcat aagtttgcga caatacatgg taagtcatac ccttggtttc caccacgtcc
     1201 aaacccgcct gacggcacat tcgcgccagc tcggcaggtg cgatgaattt tttccagtcg
     1261 tgcgtgcctt tggggacaaa cttcaacaga tattccgccg ccacaatcag atgcaggtac
     1321 gatttcgggt ttttattgat ggtggaaaaa aacaccatgc cgtccggttt gaccagcttg
     1381 gcacaggcgc gaacgatggc ggcgggatcg gggacgtgtt ccatcatttc catgcacgtt
     1441 accacatcga acgagtgcgg ttccgcctcg gcaaggtctt ccacgcggat acattcgtat
     1501 tcgatatcgg cgacattgtt caaagccgcg tgcaggcggg cggtttccag cgactgctcc
     1561 gccatgtcga taccctttac aaacgccgcg ccgcgccgcg ccatactttc cgccaagatg
     1621 ccgccgccgc agcccacatc caaaacccgt ttgccgcgca aatccgcgtg tccgtcgata
     1681 taatccagcc gcagcggatt gatgtcgtgc aaggttttga actcgcccga cttgtcccac
     1741 catttgtcgg caatccggct gaatttggcg atttccccct catcgacatt atattttttg
     1801 tcggacattt tccctcccat ctgacgaacc gcccactcca aaacccaaga ttatactatg
     1861 gaacacaatg ccgtctgaaa gccttttcgg gcggcgcggc gcaaatgcct tacaaatcct
     1921 tacactttac ggcataatgg cggctcgctt tttctggcag aaagacaaaa tatgcccaac
     1981 aaaacccctt cactgttcgg cggcgcgatg attatcgccg gcacggtcat cggcgcaggc
     2041 atgctcgcca acccgaccgc cacatccggc gtatggtttg ccggctcgct ggccgtgttg
     2101 ctgtacacct ggttttctat gctttccagc ggtctgatga ttttggaagt caacacccac
     2161 tacccccacg gcgcaagttt cgacacgatg gtcaaagacc tgctcggacg cggctggaac
     2221 atcatcaacg gcatcgccgt cgccttcgtt ttatacctgc tcacctacgc ctacatcttc
     2281 gttggcggcg acctgaccgc caaaggctta ggcagcgcct tgggcggcaa tgtttcactc
     2341 accgtcggac aactcgtctt cttcggcatc ctcgcctttt gcgtatgggc atccgcacgc
     2401 ttggtcgacc gcttcaccag cgtcctcatc ggcggcatgg tattaacctt tatttgggca
     2461 accggcggcc tgattgccga tgccaaaatg cccgtcctgt tcgacaccca agcccccgtc
     2521 ggcaccaact actggattta cgccgccacc gccctgcccg tctgccttgc gtcattcggt
     2581 ttccacggca acgtctccag cctgctcaaa tactttaaag gcgacgcgcc caaagtggct
     2641 aaatccatct ggacgggcac actgattgcg ctggtaattt acgtcctctg gcaaaccgcc
     2701 atccaaggca acctgccgcg caacgagttc gcccccgtga ttgccgccga agggcaagtc
     2761 tccgtcctca tcgaaaccct gtccaaattc gcccaaaccg gcaatatgga taaaatattg
     2821 tccctgtttt cctacatggc gatcgccacc tcgtttttag gtgtaacgct cggactcttc
     2881 gactacatcg ccgacatctt caaatggaac gacagcgtgt ccggccgcac caaaaccgcc
     2941 gcgctgacct tcctgccgcc tctaatttcc tgcctgctct tccccaccgg ctttgttacc
     3001 gccatcggct acgtcggcct ggcggcaacc gtctggacag gcatcatccc cgccatgctg
     3061 ctctaccgtt cgcgcaaaaa attcggcgca ggcaaaacct ataaagttta cggcggcttg
     3121 tggctgatgg tttgggtctt ccttttcggc atcgtcaaca tcgccgcaca ggtattgagc
     3181 caaatggaac tcgtccccgt atttaaagga taaaggcaaa atgccgtctg aagcccgccg
     3241 gcggcttcag acggcattgc cgcaacaaac ggcaaccgta ttccggcaca cagcgcatta
     3301 ccctgcccct cacgcacaaa tcccgccccg acaaaccggg acgcaaccat aaaggaacaa
     3361 tgatgaagct caaaatagac attgcaacca acaacttcaa acacggcggc ggcacggaac
     3421 gctacacatt ggatttggta aagggtctga acagacaaaa catcacaccg gccgtttatg
     3481 cgacgaaatt tgatcacggc attcctgaat acgccatgat cgaaccccat cttgtcgatc
     3541 aacaccggac gctgaaaaaa ctacgctcat tcctcttttc aagccggctc gctcaaacca
     3601 gaaaaaacag tgccgccaaa ctgattgcct gccaccacgc cgattacgcc gacctcctca
     3661 tctgcggcgg cacacacttg ggctacctgc accatatggc gcaaaaaccg aacctgctcg
     3721 accgcctcgc catacgccgt aaccgcagca actacgccac cgccaaactg attatggcgc
     3781 attcccatat gatgcggcgc gaactggtcg gactgtacgg cgttccccct gaaagaatcc
     3841 aagtcgcccc ccccccgcag atacggaacg cttcttcccg caacccggag aaactgccga
     3901 cctgcgcgcc aaatacggct ttgccgacta tgaaaccgtt ttcctgttcc catcgaccgg
     3961 ccacacgcgc aaaggtctgg aactgcttgc cgactttttc gaacataccg gcctgcccgt
     4021 caagctcgcc gttgtcggct ccccgctccc ccgccctatg aaaaacgtcg tcgggctggg
     4081 cttctgcacc gatatgcccg aactctaccg cgccgccgac tttaccatta tggcctccct
     4141 gtacgaaccc ttcgggctgg tcggtgtcga atccgtccta tgcggcacac gcgtcgtcct
     4201 ctccgaaaac atggcatgta cagaggtcat gaacgaagaa gccggattct ttttctcacg
     4261 ccaaaacccg gaaaccctgg cgcaagccgt tgcccaagcc gtcagcctta aaaaacaggg
     4321 cggacaccgc ctgtccgacc cgatgcgtgc actgaactac aacccgtccc tgtcgcacca
     4381 catcgaccga ctgaccgaca tactggcatc cgtataacaa aatgccgtct gaacgagttt
     4441 tcagacggca tttttgcaac cgttgcagcc gtgcggcaaa aagctaactg gcaattttca
     4501 aaacgtcgta cagatagttc cgatagtctc cgaccagttt ccggttcggg cagtcttcca
     4561 accctccgcc gcacaaaccg cgcatcgcca tattcgcacg catgaagccg tctttggctt
     4621 ccagaccagc cgcctccatc agcacgccgc ctgcaaaggc aaggtcgagg aaatcctgcc
     4681 gctgtacgaa tccgccggca atattgctcc tgacggcaaa ctgcgttaca taatccggct
     4741 gcgcgtaatc ccatttcttc ctgacggaca cgccctcaaa cggtacctga tgatcgccga
     4801 aataaccgaa aacaaagggt ttgccgcgtt cgtgcagata acggtcgaaa ctttccaccg
     4861 ctttgtccaa atcggcaatg ctcccgatgt agtcgttaag cgcggatacg gttttcgcat
     4921 tcaaatcagg tgcatccaaa tcaaacacat tgtcggtatc cgtccgatac ggcccgtgct
     4981 ccttcatggt cagcacatat acgaacatcg gctgccgcac gttttccaaa tcgggatggc
     5041 gtttttccaa aatcatccgc gcatactgca tcatttcctc actggaaata tgccacaggt
     5101 ttttgcccat cggcgcggga tagcccaaat cttgcggctg gaacatcaga ttaaagccga
     5161 aatggtcata tgctgccttg gcattgtagt tgcccttggt aaatggcgag agtgccacgc
     5221 aaaaataacc gtgttcgcgc aggttgcgga caaaaccggt ctgcaaatgc ggtacgaccg
     5281 agtaaaacac gccgcttgcc aacgcgccga aatccgtgga cggaacaccc gccaaaaacg
     5341 caaattcgga cttccacgtt gcgccgccga aagtatgcac gcgcaaaggt gaggaaaata
     5401 cggtatcttc ctgccgtccg aacattttca aatcgggaat cttggcggcg gcaaagtcga
     5461 aacagtgcgg atcgagcgtc gattccatca gggtaacgac aatatcgggt ttttcatccg
     5521 acatgccgca cggccgggtt tcagacggca tctgcctagc aaacgcttcc ccgtcgccct
     5581 cgaacacggg tacttggaaa aacaccgccc gacaggacat cggcaggttc aaaaacacgt
     5641 cgcgcccgtc gtccggcagc gaatccagcc acgtctttac cgcgcccgga tgtttggaaa
     5701 aatgccgcat cgacacgaac gccgccgcaa acaaaaccgc gcctgcccac cgccacggca
     5761 catccaaaga atccgcaccg ctccagccga aaaccgcata tgccgccaaa gccagcagcc
     5821 ccgccatacc gataaccgct tccttataat ggaacagcgt ttcccaattc cgccagtcgg
     5881 caatcagcaa aaagtcggaa atcaacagcg gctgtttgta ataatggatt ttcagcctgt
     5941 ggaacagtat cagcaccaca aaccagaccg aagcgaaatt caaggcacgt tgccactgag
     6001 ccgtcaacat cagcataccg cccgccaaaa agacaaacag cgcggatgcg aaaaaatacg
     6061 tccagcggta gtgcgacctg ataatcagca ccaacgccgc cgttacaaac aaaaacagga
     6121 aaacataggc aaccatcccg ccccactcct caaaacggca tacctgagcc gttgcgaatt
     6181 attatcgaaa gcgtgcatta taatgcagca caggcgcatc actgccgata tttcagtata
     6241 attattcgag atggaatcac tgaaaatggt gaagttacaa tgagcccttt tagaggaact
     6301 aaaaagtgaa ttataaaata tacgatggtg caaagattat taaaaaagaa aatttatcaa
     6361 ttggggatta ttctcaaatt gatgattttg tattttttaa ttgtggagag aaaagtattt
     6421 taggaagttt tgttcatata tcatcattta ccagtattat tggtggtgga gaattttata
     6481 tggatcattt ttcaggatta agcgcagggt gtagaattat taccggtagc gatgatttta
     6541 tggggggggg gggttaacca atccaacagt cccatctaaa tatactaatg ttaaaaaatc
     6601 tagtgttaga attggaaaac atgcaatttt aggaacaaat gtaacagtat taccaggcgt
     6661 tactattggt gatggggttg ctgttggagc aggggctata gtgagaaaaa atcttgagcc
     6721 gtggactatt tatgtcgggg tagattgtaa accactgaag cgcagatcac aagataaaat
     6781 attggagtta gagaaacatc ttgtagcaga atttaatcta gatttatgtc ctataatccc
     6841 atccccgaca cgccccaacc caaaggaaac gcgatgaaac tcatcattct cgaccgcgac
     6901 ggcgtcatca atcaggaccg cgatgacttc gtcaaatccg ttgacgagtg gatacctgtc
     6961 gaaggcagca tggatgcggt ggcatttctg acgcaggcag gctacaccgt cgccgttgcc
     7021 accaaccaat ccggcatcgg gcgcaaatat tttaccgttc aaaaccttac cgaaatgcac
     7081 gccaaaatgc accgcctcgt ccgtcaggca ggcggcgaaa tcaacggcat ctggttctgc
     7141 ccgcacaccg atgccgacaa ctgcaactgc cgcaagccca aaccgggtat gattgaagac
     7201 atcatcggac gcttcaacgc ccaagcctcg gaaacctggc tggtcggcga cagcctgcgc
     7261 gatttgcagg caatcgatgc cgtcggcgga aaacccgcgc tggttctgac cggaaaaggc
     7321 aaaaaaacgc tctcccaaca cggacacgaa ttacccgaac acacacaggt tttcgatacc
     7381 ttgctcgatt tctcacaata catcatgcag gaaaacgccg caccgcaagc cgactgaaca
     7441 taccgcattc cgacaaggca aaaccatgct catcatccgc aacctgattt actggctgat
     7501 actctgttcc accctgattt tcctctttcc ctttatgctg ctcgcctcgc ctttccgaga
     7561 cggggcgcac aagatggcgc gggtctgggt caaaatcctc aacctctcgc tcaaacacat
     7621 cgtcgggctc aaataccgca tcatcggcgc ggaaaacatc cccgaccgcc ccgccgtcat
     7681 ctgcgccaaa caccaaagcg gctgggaaac gctcgccctt caggacattt ttccgccgca
     7741 ggtttacgtt gccaaacgcg agttgttcaa aatccccttt ttcggctggg gcttgaaact
     7801 ggtcaaaacc ataggcatag accgcaacaa ccgccgcgaa gccaacgagc agctcataaa
     7861 acaggggttg gcgcgcaaaa acgaaggcta ttggattacc attttccccg aaggcacacg
     7921 ccttgcgccc ggaaaacgcg gcaaatacaa actcggcggc gcgcgcatgg cgaaaatgtt
     7981 tgagatggac atcgtccccg tcgccctcaa cagcggcgaa ttttggccga aaaactcctt
     8041 tctgaaatat ccgggggaaa tcaccgtcgt catctgtccg accatcccgc acgcaagcgg
     8101 cagcgaagcc gaattgatgg gaaaatgcga acacctcatc gaaacgcagc agccgctcat
     8161 ttccggcgca ggcccgtttg ccgccaaaat gccgtctgaa accgcatgac cgcctttgtc
     8221 cacacccttt cagacggcat ggaactgacc gtcgaaatca agcgccgtgc caagaaaaac
     8281 ctgattatcc gccccgccgg cacacatacc gtccgcatca gcgtcccacc ctgcttctcc
     8341 gtctccgctc taaaccgctg gctgtatgaa aacgaagccg tcctgcggca aacactggcg
     8401 aaaacaccgc cgccgcaaac tgccgaaaac cggctgcccg aatccatcct cttccacggc
     8461 agacagcttg ccctcaccgc ccatcaagac acgcaaatcc tgctgatgcc gtctgaaatc
     8521 cgtgttcccg aaggcgcacc cgaaaaacag cttgcgctgc tgcgggactt tttggaacgg
     8581 caggcgcaca gttacctgat tccccgcctc gaacgccacg cccgcaccac acaactgttc
     8641 cccgccccct cctcgctgac ctctgccaaa accttctggg gcgtgtgccg caaaaccaca
     8701 ggcatacgct tcaactggcg gctggtcggc gcaccggaat acgttgccga ctatgtctgc
     8761 atacacgaac tctgccacct cgcccacgcc gaccacagcc gcgcgttctg ggcactgacc
     8821 cgccgcttcg caccctacac gcccgaagcg aaacagtggc tcaaaatcca cggcagggaa
     8881 cttttcgcct taggctgacg cggcccgggc aggcatccgc caattcagac ctcctcccaa
     8941 acggacatcc ggaaagcagc atcacagccg gcgacacata ccgctgccgc cgctttcaga
     9001 cggcatccgt gccggaacag gcacgcgccc gcccgattca aaccgcgatg acgctttgcc
     9061 gccggttcgg ggcaggatgg cggcacacgc gccgtctgcc gcgtttcatt tcacaccgct
     9121 cttccgaaac ccgaaacccg cccggtccga cgtgcggtat gaaacgctta agctggcgcg
     9181 aagtctttta ctgatttgcc cgcgaaaatg ccgtctgaaa ggttttcgga cggcattttt
     9241 ttgcgtttcc caggatggcg gcggattcgt aaaaggcggt cagggtggat tgtaggatgg
     9301 gttgagacct gccgaatccg ccgcatctgc caaatctacc gccgtcattc ctacgaaagt
     9361 gggaatctag aacgcggggt taagaaaacc tgcaccgcgt cattcccacg aacctacatc
     9421 ccgtcattcc cacgaaagtg ggaatccaga acgtaaaatc tgaagaaacc gttttacccg
     9481 ataagtttcc gcaccgacag a
//
LOCUS       DAWKQB010000074         9419 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.074, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000074 DAWKQB010000000
VERSION     DAWKQB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.074"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(113..1555)
                     /gene="aldA"
                     /locus_tag="WJL14_001312"
     CDS             complement(113..1555)
                     /gene="aldA"
                     /locus_tag="WJL14_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ7656373.1"
                     /translation="MKQLAMYINGRFENDFNGEWRNVLNPSTEEAIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1590..1790)
                     /locus_tag="WJL14_001313"
     CDS             complement(1590..1790)
                     /locus_tag="WJL14_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656374.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVVYLGIN"
     gene            complement(1799..2188)
                     /locus_tag="WJL14_001314"
     CDS             complement(1799..2188)
                     /locus_tag="WJL14_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656375.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2396..3313)
                     /locus_tag="WJL14_001315"
     CDS             complement(2396..3313)
                     /locus_tag="WJL14_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656376.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRILNAAFAGGA
                     HQVNRLADTLNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3486..4286
                     /locus_tag="WJL14_001316"
     CDS             3486..4286
                     /locus_tag="WJL14_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656377.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGKVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4334..5110
                     /gene="mlaE"
                     /locus_tag="WJL14_001317"
     CDS             4334..5110
                     /gene="mlaE"
                     /locus_tag="WJL14_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ7656378.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5161..5655
                     /gene="mlaD"
                     /locus_tag="WJL14_001318"
     CDS             5161..5655
                     /gene="mlaD"
                     /locus_tag="WJL14_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ7656379.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5692..6282
                     /locus_tag="WJL14_001319"
     CDS             5692..6282
                     /locus_tag="WJL14_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ7656380.1"
                     /translation="MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6339..6617
                     /locus_tag="WJL14_001320"
     CDS             6339..6617
                     /locus_tag="WJL14_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ7656381.1"
                     /translation="MHTELNNGTLHISGDVTVKTLTAAAFTRFRQQCRLKDTRAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6614..7453
                     /locus_tag="WJL14_001321"
     CDS             6614..7453
                     /locus_tag="WJL14_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ7656382.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPG
                     TQP"
     gene            7578..7940
                     /locus_tag="WJL14_001322"
     CDS             7578..7940
                     /locus_tag="WJL14_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ7656383.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7937..8323
                     /locus_tag="WJL14_001323"
     CDS             7937..8323
                     /locus_tag="WJL14_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7656384.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8320..8808
                     /locus_tag="WJL14_001324"
     CDS             8320..8808
                     /locus_tag="WJL14_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ7656385.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8890..9399)
                     /locus_tag="WJL14_001325"
     CDS             complement(8890..9399)
                     /locus_tag="WJL14_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7656386.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGPVVGKNVMMEPECLLYSTNHKFDRENK
                     RFEGYTEIRPITLENDVWPGRRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPA
                     VVKKNLPEG"
BASE COUNT         2164 a         2644 c         2377 g         2234 t
ORIGIN      
        1 agacaaaaac atcgacctcg gtcatgactg attgccgatg aagcaataaa aatgccgtct
       61 gaacctgaaa acgggttcag acggcatttt ctatcggggt tttaaagcgg cattaaatgt
      121 cggtttccaa ataaacgact tgggtttgca gatattcttc caaaccgtgt ttgccgtccg
      181 cgccgccgat accggatttt ttccaaccgg cgtggaaacc ctgcatggct tcgaagtttt
      241 cgcggttgat gtaggtttcg ccgaattgca ggcggcgggt aacgtagaag gcttcgttta
      301 aattagtcgt ataaacagaa ctggtcagac caaactcgca atcgttagcc agtgcgatga
      361 cttggtcgag cgtgtcgaaa gcggaaacgg gcagcacggg gccgaaggtt tcttctttca
      421 taatgtccat actgttgtcg gtgtcggtca gcagggtcgg ctcgaagaaa tagccgcgcc
      481 cttcggcgcg tttgccgccg caaaccaatt tcgcaccttg tttgactgcc cgttccactt
      541 tttcggcaac ggctttgacg gcgcgctctt caatcagcgg gcccatttcc aatgcgcctg
      601 cttcggcttc ggcagggttg ccgtagcgca cgcctttcat cgcggcggtc attttttcga
      661 taaacgcgtc tttcaggctg ctgtggacat agacgcgctc ggcgcagttg cagatttgac
      721 cggtgttgcc gacgcgcgaa gccaagatgg atttcaccgc caaatccaaa tccgcgtctt
      781 tcaacacgat ggcgggggct ttgccgccga gttccaacga aactttggtg atgttggcgg
      841 aagcggcttc catcacttgg cggcccgctt cgacggagcc ggtcaggctg accatatcga
      901 cttgcggatg ggcggacaag gcattgccga tttccgcgcc cgggccgttc accacgttga
      961 acacgccggc gggcagtccg accgcatcga cgatttcggc gaagatgtgg cagttgatcg
     1021 gggttacgct gctgggtttg acgacgatgg tattgcccgt taccaaagcg gggcccattt
     1081 tgcgggcaat caggaagaag gggaagttcc acggcaaaat gccggcaatt acgcccagcg
     1141 gacgtttgaa caataaaata ttttcgcgcg gacggtcgct ttggatgatt tcgccttcgt
     1201 aacggcgcgc ccattcggct tggtaatcga gatagtcggc ggtgaacatc acttccacgc
     1261 gcgccaagtc tttggttttg ccgccttcgg cgacaatggt gtcggtcagc tcgtcggcgc
     1321 gttcgcgtat gccttgggcg attttgcgca aatacgcgcc gcgttcgacc gcaggcagac
     1381 gctcccaagc cggttgcgcc gcacgcgccg ccgctacggc gcggtcaacg tctgctttgc
     1441 cgcctttggg ttcgcgggcg atggcctctt cggtggacgg gttcaatacg ttgcgccatt
     1501 cgccgttgaa atcgttttca aagcgtccgt tgatgtacat ggccaattgt ttcatttcag
     1561 gttctccggt tttgtagtca gatgtagttt tagtttattc ccaaataaac taccgtacaa
     1621 gtttcttttg aaatgtttag acgagaagct actatgtctg catattgttc tgctaattta
     1681 tattcactcg aatgcaactg ccgattattc caatctgcat tcgcaccagt aaaagcccca
     1741 gcattgccgc ctgtggcata accaatcgct aaactgccaa cggtatccac aacaggtttc
     1801 aggctgcctg aatttctcta ataaatgaaa cacttattct atcaaccgtt aatgtaacca
     1861 aacaattatc aagataagag tagtttatta aatactcatc ttcttcaaat tgtatatttt
     1921 gtagggttag atgattattt tttaattctg ccgttatttc atacttgaaa ctatcatcta
     1981 tttttctaaa tccaatattt gaagttgatt tatccagtat gtaatcatta aagatttcat
     2041 aggataattc cacttcataa aattgatttt caaatatttt gaatgggcag tacgatataa
     2101 aacaatgtag ttcataacca tttatttcaa tcaatactgc ctcttcaatg gtgttatcta
     2161 actttttgat tttacattta tataacattt atcttgcctt tggataaatt gttttgtttt
     2221 ttggattgtt attcaaagtt ctccaaaagt cttttcctga ttttgtatgt atcgtggtaa
     2281 tactaccatt ttcattggtt ccaacaaaaa taaatttaga ttgacctttg gaatcagtac
     2341 ccaaaaactg aacatatcca gacatgcgga tttcgggttt taaatcaaat gcggcttacc
     2401 ggttttcttt tttggtttcc gcaggcgtgc tgccgaaata ttgcctgaat gcctgagtaa
     2461 agctggaaac gtggcggtag ccgcataaat atgcggtttc gcctatgctt tttcccccgt
     2521 tttgcaacag atagagcgcg tgctgcattt gtttgtggtg cagccattcg cttgccgtaa
     2581 tgccgaaatg gtcgcgcata cggcgttgca gcgtcctttc gctgatgttc agcgtgtctg
     2641 ccagccggtt gacttgatgt gcgccgccgg caaacgcggc attcaggatg cggctgaagt
     2701 cttcagacgg catagcgtct gcttccgccg tttgccccgc cgccggcccg atgccgtctg
     2761 aaaccgtgtc ccacaagtcc gacagcagcc gcaacacgtc cgcctcgcgg cgcaatgttt
     2821 cgcccaaatg cccctttggg acggctttca ggcaggatgc cgccaagccg cgcaggttcg
     2881 ggggcaaatc ccatatcctg accggttcgc ggtaaagcag gggtgcgaaa cgcgcgtatt
     2941 ccggacgcag cagccattgt tccatacctt taattgtcat tttgaccgtt ttgccgcctc
     3001 ggtaaagata gcggctgaac aggacttctt ccccgacagc aattaggaca atcttgccgc
     3061 cgtccgcatc gatttggaag cggcagcggt tgatgccgaa gtccaaactg ccttccagca
     3121 agagcacgaa cgacacataa ggttcggcga ggcggctgct gcaaaaatca caccgtgccg
     3181 ttactgtgcc gccgtggatg gaaatgccgt tggacaaagt atcgaaacgg tagctgccct
     3241 ccacatagct gccgccgccg cttttgacta gattgacgaa ccggctgctg ttcaaatgtt
     3301 ctgctttttc catagttttc tgataaaggt tatcatttga aagataacat ttttcgccac
     3361 agcaaacaat ccgccgccga caacggcatt ttcacaacat ttaccgtccg tatgccgtct
     3421 gaaaattgcc cgtcttcccg ataagcctta aaatacacgc cgtcaaacct accgtccgtc
     3481 cgtacatgag cccatccccc tttatcgaaa tgaaagacgt cgcttttgcg tatggcgacc
     3541 gcccgattct gaacgacatc aatttcagca ttccgcaagg caattttgcc gccgtgatgg
     3601 gcggttcggg cagcggcaaa accacgctga tgcggctgat tacgggtcag atccgtccgc
     3661 agtcgggcaa agttctgata gaagggcgtg atttggcggg cttttcggct gacgaactct
     3721 acgaacaccg ccgccgcatg ggcgtattat tccaacacgg cgcgctgttt accgatttgt
     3781 cggtattcga caatatcgct tttccgatgc gcgaactgac acgcctgccg gaagcggtga
     3841 ttcgagattt ggttttgttg aaactgaacg cggtcggttt gcgcggcgtg gaaaacctga
     3901 tgccgtccga gttgtccggt gggatgtcgc gccgcgtcgc gcttgcccgc acgattgcgc
     3961 tcgaccctga aattatgttg tacgacgaac cgtttaccgg cctcgatccg atttccttgg
     4021 gcgtgattgc ccacttgatc agccgcgtca ataaggcttt gcgttcgacc agcgttatgg
     4081 taacgcacga cattgaaaaa tctttggaaa tcgtcgatca ggtgattttt ttggcgcacg
     4141 gcgaaattat gttctccggc tcgccgcagg aaatgcgcga actggattcg ccttgggtgc
     4201 gccagtttgt cggcgggctg gcagacggcc ccgtagcata ccgctatccg gcgcaaacgt
     4261 cgttgcagca ggatttgctc gggtaaatga gcaggtgccg tctgaaaccg ttgaacttta
     4321 catatagaaa tttatgaatt ttatccgttc cgtcggggcg aaaaccctcg gccttattca
     4381 atctctcggc agtatcacgc tgtttctgct gaatattctg gcgaaatccg gtacggcttt
     4441 cgtccgtccg cgcctgagcg tgcgccaagt gtattttgcc ggcgtgctgt cggtgttgat
     4501 tgttgccgtt tcagggctgt ttgtcggcat ggtcttgggt ttgcagggct atacgcagtt
     4561 gtcgaaattc aaatccgccg atattttggg ctatatggtc gcggcttcgc tgttgcgcga
     4621 actgggtccg gtgttggcgg cgattctgtt tgccagcagc gcgggcggtg cgatgaccag
     4681 cgaaatcggt ttgatgaaaa cgaccgaaca gctcgaagcg atgaacgtga tggcggtaaa
     4741 ccccgtcgcc cgagtggttg cgccgcgctt ttgggcgggc gtgttttcca tgccgctttt
     4801 ggcttcgatt ttcaacgtgg cgggtatttt cggcgcgtat ttggtcggtg taacctggct
     4861 gggcttggac agcggtattt tctggtcgca aatgcagaac aacatcacga tacattacga
     4921 tgtaatcaac ggtctgatca aatccgccgc gttcggcgtg gcggtaacgc tgattgccgt
     4981 gcatcagggc ttccactgcg tcccgacctc ggaaggcatt ttgcgcgcca gcacgcgcac
     5041 ggtggtttcg tccgccctga cgattttggc ggtcgatttt atattgaccg cgtggatgtt
     5101 tacagattga caggccgtct gaagacgaaa cgcggaacat cggatattca aggaacttta
     5161 atgaaaaaga acatattgga attttgggtc ggactgttcg tcctgattgg cgcggcggcg
     5221 gttgcctttc tcgctttccg cgtggccggc ggtgcggcgt tcggcggttc ggacaaaact
     5281 tacgccgttt atgccgattt cggcgacatc ggcggtttga aggtcaatgc ccccgtcaaa
     5341 tccgcaggcg tattggtcgg gcgcgtcggc gctatcggac ttgacccgaa atcctatcag
     5401 gcgagggtgc gcctcgattt ggacggcaag tatcagttca gcagcgacgt ttccgcgcaa
     5461 atcctgactt cgggactttt gggcgagcag tacatcgggc tgcagcaggg cggcgacacg
     5521 gaaaaccttg ctgccggcga caccatctcc gtaaccagtt ctgcaatggt tctggaaaac
     5581 cttatcggca aattcatgac gagttttgcc gagaaaaatg ccgacggcgg caatgcggaa
     5641 aaagccgccg aataaacgta tgccatcata ttcaaactag gacacttaat catgaaaaaa
     5701 tcctccttca tcagcgcatt gggcatcggt attttgagca tcggcatggc atttgccgcc
     5761 cctgccgacg cggtaaacca aatccgtcaa aacgccactc aagtattgag catcttaaaa
     5821 agcggtgatg ccaacaccgc ccgccaaaaa gccgaagcct atgcgattcc ctatttcgat
     5881 ttccaacgta tgaccgcatt ggcggtcggc aacccttggc gcaccgcgtc cgacgcgcaa
     5941 aaacaagcgt tggccaaaga atttcaaacc ctgctgatcc gcacctattc cggcacgatg
     6001 ctgaaattaa aaaacgccaa cgtcaacgtc aaagacaatc ccatcgtcaa taaaggcggc
     6061 aaagaaatca tcgtccgcgc cgaagtcggc gtacccgggc aaaaacccgt caacatggac
     6121 ttcaccacct accaaagcgg cggtaaatac cgtacctaca acgtcgccat cgaaggcgcg
     6181 agcctggtta ccgtgtaccg caaccaattc ggcgaaatta tcaaagcgaa aggcgtggac
     6241 ggactgattg ccgagttgaa ggctaaaaac ggcagcaagt aatccataag ccatgccgcc
     6301 agactgaaga tggcatggat tttttcctac cgaacactat gcatacagaa ctcaacaacg
     6361 gaacactgca catcagcggc gacgtaaccg tcaaaaccct gaccgccgcc gcctttaccc
     6421 gcttcagaca gcaatgtcgt ctgaaagaca cccgcgcggt tgatttgagc ggcgtcggac
     6481 gcgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct gcgcggttgc aaaggcagcg
     6541 tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc cgaactgtac gaaatcaaag
     6601 actggctgaa atcatgaaaa aaaccgccta tgccttcctc ctgctgatcg ggttcgcttc
     6661 cgcccctgca tttgccgaaa cccgccccgc cgacccttat gaaggctaca accgcgccgt
     6721 tttcaaattc aacgaccaag ccgaccgcta cattttcgcc cctgccgcgc gcggctaccg
     6781 caaagttgcg ccgaaacccg tccgcgccgg cgtgtccaat ttttttaaca acctgtgcga
     6841 cgtggtcagc ttcggcagca atatcttgcg cttagacatc aaacgcgcaa gcgaagacct
     6901 tgtccgcgtc ggtatcaaca ccactttcgg tttgggcggg cttatcgaca tcgccggcgc
     6961 gggcggcatt cccgacaata aaaacacctt gggcgacacg tttgcttcgt ggggatggaa
     7021 aaacagcaat tatttcgtgt tgcccgtctt agggccgtcc accgtccgcg acgcgctcgg
     7081 cacgggtatt acctccgttt attcgcccaa gaatatcgtc ttccgcaccc ctgtcggacg
     7141 ctggggcacg actgccgtat ccgccgtcag tacgcgcgaa ggcctgctcg atttgaccga
     7201 cagtctggac gaagccgcca tcgacaaata cagctacacg cgcgacctct atatgaaagt
     7261 ccgtgcgcgg cagaccggtg caacacctgc cgaaggtacg gaagataaca tcgacatcga
     7321 cgaattggtc gaaagtgccg aaaccggcgc ggcggaaact gccgttcaag aagattccgt
     7381 atccgaaaca caggcagaag cagcagggga agccgaaacg caacctggaa cacaacctgg
     7441 aacacaacct taaagccatg ccgtctgaaa atccgtgttc ggacggcatt cccttctgtc
     7501 ggaacggaca gtccgaaacc acagattggg ctataatctc ttttttgcgc ataccgcgcg
     7561 acagcaaagg aaaaaccatg tacgaagtca accgcagcgt attcgtcctc atccctttag
     7621 aacctttttg gaactggctg caaaccctgc cgggcaacca cctcgacgga ctgactcttg
     7681 aagacatcca agccgacgcc aattcctacc ttgtccgccc gtgcgaaacc gccgacgaag
     7741 tatgggacga aatcgaagcc cgttttgaag acattttcgc cgccgaactt gccgactggt
     7801 gcgaagacga acgcgagtgg cccgcgctcg atgccgacat tttcaacgaa tggttcgaca
     7861 tccagctttc taccgtcatc accgacctcg aacacgaacc gcttgcccgc gaagccttcc
     7921 aacccatcaa cctgaactga tgaagctgac cgtccgcaac taccatctcg acggctacgg
     7981 ccatgtcaac aatgcgcgct acctcgaatt tttcgaagaa gcgcgctggg cgttttttga
     8041 agaacgcgga ctgctgcacg agttggcagg cctcatactg attgtcgccc gcatcgacat
     8101 ccgatacagc cgccccgccg tcgaaggcga cgtattgcag ttttcatgcc gcctgaaaac
     8161 ccccggcacg cgccgtattg tgctgaccca gacgattaca ctgccaaacg gcaaaaccgc
     8221 cgcagaagcc gacattaccc tgatgcccgt ccacgccgcc acacaacgca ccgtcagcct
     8281 gcccgccacc cttgcccgcg cattggaagc cttgtccgaa tgaaaaaaat actcaccgcc
     8341 gccgtcgtcg cactgatcgg catcctcctt gccatcgtcc tcatccccga cagcaaaacc
     8401 gcgcccgctt tctccctgtc cgacctgcac ggaaaaaccg tttccaacgc cgacctgcaa
     8461 ggcaaagtta ccctgattaa tttttggttt ccctcctgtc cgggttgtgt gagcgaaatg
     8521 cccaaaatca ttaaaacggc aaatgactat aaaaacaaaa acttccaagt ccttgccgtc
     8581 gcccagccca tcgatccgat agaaagcgtc cgccaatatg tcaaagacta cggtttgccg
     8641 tttaccgtca tgtatgatgc ggacaaagct gtcggacagg cgttcggcac acaggtttat
     8701 ccgacttccg tccttatcgg caaaaaaggc gaaatcctca aaacttatgt cggcgaaccc
     8761 gatttcggca aactctacca agaaatcgat accgcgctgg cacaatagcg atgccgtctg
     8821 aagcgcaaaa aaacagaaac gatgttcctg ttttttcaga tcatccgccc cgacacgttc
     8881 agacggcatt caaccttccg gcagattctt tttcaccact gccggattgc ctgccgccaa
     8941 agagtagggc ggaatgtctt ttgtaaccac cgcgcctgcg cccacgaccg agccgcgtcc
     9001 gacggttacg cctgccatta caatcaccct gcgccccggc cagacatcgt tctccaacgt
     9061 aatcggacgg atttccgtgt agccctcaaa gcgtttgttt tcacggtcaa acttgtggtt
     9121 ggtcgaatag agcaggcatt ccggctccat catcacattt ttgccgacca ccggcccacg
     9181 gcagatttca cagtttgccc cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac
     9241 atacgccccg cgttcgatat tgaccccgcg tccgatatgc ggagaaaccc gccgcgccaa
     9301 aaaaccgcgt acccgtctgc cgacgatgcc gcgcatatgc gacggcggca acaccgaacc
     9361 ggcaaaacgc agcagcagga aaaagaattt tgatcccatc aaacctccct caaaagaac
//
LOCUS       DAWKQB010000075         9355 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.075, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000075 DAWKQB010000000
VERSION     DAWKQB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.075"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..174)
                     /locus_tag="WJL14_001326"
     CDS             complement(<1..174)
                     /locus_tag="WJL14_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656387.1"
                     /translation="MPSERLSDGIFPCVLNQVHETPDAFGGNVGCRTMFGGDQRTKCA
                     GKGLMPSEGSDGIG"
     gene            complement(192..749)
                     /locus_tag="WJL14_001327"
     CDS             complement(192..749)
                     /locus_tag="WJL14_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ7656388.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(825..2459)
                     /locus_tag="WJL14_001328"
     CDS             complement(825..2459)
                     /locus_tag="WJL14_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ7656389.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2863..3969)
                     /gene="serC"
                     /locus_tag="WJL14_001329"
     CDS             complement(2863..3969)
                     /gene="serC"
                     /locus_tag="WJL14_001329"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ7656390.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPCLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSDGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4334..4765
                     /gene="rimP"
                     /locus_tag="WJL14_001330"
     CDS             4334..4765
                     /gene="rimP"
                     /locus_tag="WJL14_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ7656391.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4793..6295
                     /gene="nusA"
                     /locus_tag="WJL14_001331"
     CDS             4793..6295
                     /gene="nusA"
                     /locus_tag="WJL14_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ7656392.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6307..9195
                     /gene="infB"
                     /locus_tag="WJL14_001332"
     CDS             6307..9195
                     /gene="infB"
                     /locus_tag="WJL14_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ7656393.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAATRAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKNGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKT
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILMVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMIKGYNEIMEGDQLECFDIVEVARTL"
BASE COUNT         2501 a         2693 c         2382 g         1779 t
ORIGIN      
        1 accgatgccg tctgaacctt cagacggcat gagacctttg cctgcgcact tggtgcgctg
       61 gtcgcctccg aacatggtgc gacacccgac atttccgccg aacgcatcgg gcgtttcatg
      121 aacctggttt aaaacgcatg gaaaaatgcc gtctgaaagc ctttcagacg gcattttgct
      181 tgagattccg tttaccaatg gctgacaaac gcttccaaat cggtattctt gggcttatgc
      241 acttcctctg tcggcgtgcc gaccatcatc agcccgatga ttttatcctt atccgcacat
      301 ccgaaagcct cacgcaacag ggggctgttg acccacatcc ccgttatcca gacattgtcg
      361 aaaccttgcg ccgttgccgc cagttgcagc gcatacgccg cacaccccgc cgtcagcatc
      421 tgctcccatt ccggtttcgg cttaggcaca tcgcggttcg gcgcaaacgt taccccgatg
      481 accatcggcg ccatattgcc cactttttcc gcctttttca tcgcatcgtc gccgaaattc
      541 aattcggcaa ccgtttgctt taacacatcg cgaaaacgtt gcaatcctac ctcgccttga
      601 atcacggtaa aacggaaggg gcgcatattg ccgtgatcgg gaacttgggt tgccgcctga
      661 aatatttgtt ccaactccgc cgcatcgggg gcggggtgct tcagcttttt ggaagatcgg
      721 cggttcgtca ataattttaa agcatccata tcgttatact ccggtcatcg gtcgggtgtt
      781 cggacacata tgccgtccga aggcttcaga cggcatatcc ggcatcagcg cggacggcgg
      841 caggctgcca atatatccat ttccttccga taggtttggc tgttggaaat gtccatcagc
      901 cccaataccg tgctgaaata gtggtcgtgc gaatattcgt tttccgccgc tttttgtttg
      961 aggcattgga aatctatgcc cccgtgttgg cggaaggctt tggaaaacca cataaccatc
     1021 gggatatgcg tctgcccgga aggcgcgatg gcgtaaggcg cggcgtgcag gtacatcccg
     1081 ttttcgccca aactttcgcc gtggtcggaa acataatgca ccacgctttc caaatcgtcg
     1141 cggttttcaa gtttgcggat aaccttgtcg ataaactggt ccacatacaa aaccgtattg
     1201 tcgtaagtgt tgaccagcgt ggcgcgggtg catttgttga tttcgttggt gtcgcaggtc
     1261 ggcgtgaatt tgcgttcggc ttcggtatag cgttcgtaat acgtcggccc gtggctgccg
     1321 atggtatgca ggattaaaac cgcgtcttta tcgtttttgt tgaggacttc gtcgaactta
     1381 gtcagcagga tattgtcgag gcactcgccg ttgcggcagt attcgggcag gttgagcgag
     1441 gtaacgtcgg tattcggcac tttgccgcac acgcccttgc agccggaatc gttttccaac
     1501 caggtaactt ccacgccggc gcgctgcacg atgtccagca ggttgtcttg gtgttcggct
     1561 ttgatttcgt cataatccgt gcggtcgaag gttgagaaca tacacggcag ggagtgcgcg
     1621 gtcgatgtgc cgcagcttct gacctgcggg aaattgacaa tttcatcgcc gcgcgcggca
     1681 agcagcggcg tagtttggcg gctgtaaccg ttcaaacccc agttggcggc acgcgtggtc
     1741 tcgcccacga ccagcaccac gaaacggcgc aggctgccgg ccggccggtt ttgcacgacc
     1801 gccatatcca attgcgtata aggaatattg gaacgcttcc aatctttgta tttcgacacg
     1861 cccgcgccga tgaaattaga cggcacaatc agatgggtta ctgatttatt gttgcggaaa
     1921 aacgaggcgt aatcctgata ttgcaacatt gcgatgccca acgcgcacaa aaaggaaacg
     1981 gcggcaagca caaggcgcgt caaaagctcc ttataccaaa cgcggtattt aaccttgacg
     2041 gcgatatacg ccagcgcggg caatacgccc aaacatacaa tccacagcac atagcccggc
     2101 gtaatcaggc gcgcgctttc ggcagccgta gtttgcaaga cattattcaa catcgacttg
     2161 ttgaaataga tattgaaaaa tatttcttgg taagacaccg ccgcactgat aaccaatatc
     2221 aacggaatca ataccttatg cacgaaaggc agggcaatga cgtgaaaaac gaaattactt
     2281 aaaaaaaaca gcaccaccgg catcgtatag aggaagatat ccgccccggt gccgttaaaa
     2341 ggatgaagct cgacaacttt ggcaaaaaag gcgtaattca ataccagcga ggaatacagg
     2401 gaaaggaagg caatcagcgc ggaagagccg agcttcggcc tcaggttcgg ttttatcatt
     2461 tggaatgtgt cggataaggg ttggaaaagg catccggcat ttggaatccg gattattgaa
     2521 aaagattctt aattataagg caacggagca aagcagggca agaaaacggc ggctgtgcgg
     2581 gggggttccg cccgccattc aaacgtccgg cagacataaa aacatcgtaa gcaagattcg
     2641 aaccggtctg caaccgcccc tgccagaaaa acgggcaaag catttcatat tggaaaaacc
     2701 cggccgcgcc gccgacggga cagtccggca caaacagcat cacgctcgga ttgaaaagga
     2761 cggatagccg ccggcagcac aatcttacca cctccaaaac gccgccggag aacgccgaaa
     2821 cagccgatgc cgtctgaagc cgcttcagac aacatcggga catcaaccgt aacgccgttg
     2881 gaaatcgcgc ataaaatctg ccaaagcccg cacgccttca agcggcatcg cattataaat
     2941 gctggcacgc ataccgccga cggttttata gcccttaagc aggcacaagc cctgcaattc
     3001 ggcttccagc acaaaacggc ggtcaagctc ctcatccccc gtttggaaca cgacattcat
     3061 tttagaacgc gcattcggac ggatacggtt gatataaaaa ccatcgctgc cgtctatcgt
     3121 ctcatacaag gtttgcgcct tcagccgatt gaccgcttca atttttttca caccgccctg
     3181 cgcctgtagc cagcggaaca ccagccccga catataaatc gcgtaagttg acggcgtgtt
     3241 gtacataccg tcgcggttca aatgcgaacg gtagttgaac acatcgggaa tatcgttcgg
     3301 acaacgctcg agcaaatcct cacgcacaat caccaccgta actcctgccg gcccgatgtt
     3361 tttctgtgcg cctgcgtaaa tcagtccgta gtcggcaaca tcaaactcgc gcgacaaaat
     3421 ctcgctggac atatcgcaca ccagcggcgg catgccttct gaaaggcacg gcacttcacg
     3481 gtattgcagc ccgttgaccg tttcattgac ggcaaaatgg acaaacgccg aatcgggtgc
     3541 aacatcccac gtttccacag gcggcaggtc gagatagtcg aactgctcgc cgccatgcgc
     3601 cgccaaacgg atttccgtat cggtcaaacg gctcatctgt tcataagcga tacggctcca
     3661 gttgcccgtt accaccgcgt cggcagtgcg gaaaccgtgt gccagattca tggctgccat
     3721 attaaattgg gttgttgctc cgccctgcag aaacaatatc ttatagttgt caggcacttt
     3781 caaaagctgc ctcaaatcct gttccgcatg atgcaggatg ctcaaaaaca tttccgaacg
     3841 gtggctcatt gccatcacag gaaaacccgt accgttgtag tccaacattt cctgccgcgc
     3901 cgtttccaac acggcttcgg gcaatacggc agggccggcg gaaaaattgt aaatcggata
     3961 aagagacatg atgcagcctt gattctgaac aataacccgc ccgattttag gcttgcagca
     4021 ggctttgtgc aaggatggaa aatacctgtc ctccgcccga ttccatgccg cccgaacacg
     4081 gaaaataata tcaatatatt gatttacaaa cataaaaatc atgcacgcga caaatagata
     4141 catttgtttt gtcaacaata ttcacgattt cccattacaa acctccctta cacccgcttt
     4201 tttccgtccc aaaaacacaa aataaatcaa cactttcatt tctccgcaaa agcggttata
     4261 atcacgccga tttttcaact ttgacgaaaa atggccacgg ggccattttt ttattatgta
     4321 catagggagc agcatggata tccaaaccat cctcgaaaaa accctgcccg gcctgggcta
     4381 cgaactggtc gatttcgaac tgaccgcgca aggaacattg cgcgtgttca tcgacaaaga
     4441 aagcggcatt accgtcgaag actgcgcaac cgtcagcaac cacttgagcc gcgtcttcat
     4501 ggttgaagac atcgactaca aaaacctgga aatttccagc cccggactcg accgcccctt
     4561 gaaaaaagcc gccgacttcg tgcgctttgc cggtcagaat gccaaaatca aaacccgcct
     4621 gccgatagac ggtcagaaaa actttatcgg taaaatcgaa ggctgcgaaa acgataccgt
     4681 taccgtatcc ttcgacggca aaaccgtaca aatcgaattg ggcaacatcg acaaagcccg
     4741 tctgcgcccc gaattcaaat tctaaaacac aacaatattg gagatgttca aaatgagtcg
     4801 tgaaatgtta cagctggcag aagcactggc aagcgaaaaa aacgttgatg cggaagtcgt
     4861 cttccaagca ctggaattcg ccctgtctac cgccgccaag aaaaaggcag accgcgaaca
     4921 catggacgtg cgcgtccaaa tcaaccgcga caccggcgaa taccaaacct tccgccgctg
     4981 gctgattgtc gccgatgaag actataccta tcccgatgtc gaaaaaacca tcgaggaaat
     5041 ccaagaggaa attcccggca ctaccatcca aatcggcgaa tactacgaag agcagctgcc
     5101 caacgaaggc ttcggccgcc aagccgcgca aaccgccaaa caaatcatcc tgcaacgcat
     5161 ccgcgatgcc gagcgcgagc agaatctgaa cgagtttctc gccgtcaaag aagacatcgt
     5221 gtccggcacg gtcaaacgcg tcgaacgcca cggcatcatc gtcgaagtcg ttgccggcaa
     5281 actggacgcg ctgattccgc gcgaccaaat gattccgcgc gaaaacttcc gcagcggcga
     5341 ccgcatccgc gccctcttcc tgcgcgtcga agaaatcggc aacaccggcc gcaaacaagt
     5401 catcctgagc cgtacttccg gcgatttcct cgtcaaactg tacgccaatg aagtacctga
     5461 aattgcagac ggcatgcttg aaatccgcgc tgtcgcccgc gacccgggcc agcgtgccaa
     5521 agtcgccgtc aaagccaacg accagcgcat cgatccgcaa ggcacctgta tcggcgttcg
     5581 cggttcgcgt gtcaatgccg tcagcaacga attgtccggc gagcgcatcg acgttgtcct
     5641 ttggtcgccc gagcctgccc aattcgtcat gagcgcgctc tcacccgccg aagtcagccg
     5701 catcgtcatc gacgaagaca aacacgccgt cgatgtcatc gttgccgaag accagctcgc
     5761 gctcgccatc ggacgcggcg gtcaaaacgt gcgccttgct tccgacctga ccggctggca
     5821 gctcaacatc atgacttccg ccgaggcaga cgaacgcaat gcggcagaag atgccgccat
     5881 ccgccgcctg tttatggatc acttgaacgt ggacgaagaa accgccgacg tactggttca
     5941 ggaaggtttt gcaaccttgg aagaagtcgc ctatgttcct gccgccgaac tgcttgccat
     6001 tgaaggattt gacgaagaaa tcgtcgatat gctccgcaac cgcgcccgcg atgccatcct
     6061 gaccatggcg attgccgccg aagaaaaact gggcgaagtg tccgacgata tgcgcaacct
     6121 cgaaggcata gatgccgata tgctccgcag ccttgccgaa gcaggcatta ccacccgcga
     6181 cgacttggca gagcttgctg tggacgaact gattgaaatc accggtgtaa acgaagaaac
     6241 cgcaaaagcc gtcatcctga ccgcacgcga acactggttt accgaagaca aataaagggg
     6301 gtacagatga gtaacacaac cgtagaacaa tttgccgccg agctgaaacg ccccgtcgaa
     6361 gacctgttga aacagttgaa agaagccggc gtcagcaaaa acagcggcag cgattccctg
     6421 acgctggacg acaaacagct tctgaacgcc tacctgacca agaaaaacgg cagcaacggc
     6481 ggcaccatca gcatccgccg caccaaaacc gaagtcagca ccgttgacgg cgtaaaagtc
     6541 gaaacacgca aacgcggacg cactgtcaac attccttctg ccgaagagtt ggcagcacag
     6601 gtaaaagccg cccaaaccca agccgcacct gtccggccgg agcagacggc agaagacgcg
     6661 gcaaaagccc gagccgaagc tgccacacgt gcagaagcac gtgccaaggc agaagcggaa
     6721 gcggcaaaac tgaaagcggc aaaagcaggc aacaaagcca aacctgccgc gcagaaaccc
     6781 accgaagcaa aagccgaaac cgcacccgtt gcggcggaaa ccaaacccgc cgaagaaagc
     6841 aaagcggaaa aagcccaagc cgacaaaatg ccgtctaaaa aacccgccga gcccaaagaa
     6901 aaagccgcca agccgaaaca cgagcgaaac ggcaaaggca aagatgccaa aaaaccggcg
     6961 aaacctgccg cacctgccgt gccgcaaccc gtggtcagcg cggaagaaca ggcgcaacgc
     7021 gatgaagaag cacgccgtgc cgccgcactt cgcgcccacc aggaagccct gttgaaagag
     7081 aaacaggaac gccaggcacg ccgcgaagcc atgaaacaac aggcagaaca acaggcaaaa
     7141 gccgcacagg aagccaaaaa cggcagacag cgtcccgcca aacctgccga gaaaccgcag
     7201 gcagccgcgc cggccgtcga aaacaaacct gtcaatccgg caaaagcgaa aaaagaagac
     7261 cgccgcaacc gcgatgacga aggtcaaggc cgcaacgcca aaggcaaagg cggaaaaggc
     7321 ggacgcgacc gcaacaatgc acgcaatggc gacgacgagc gcgtacgcgg cggcaaaaaa
     7381 ggcaaaaaac tcaaactcga gccgaaccaa cacgccttcc aagcaccgac cgaacctgtc
     7441 gtacacgaag ttttggtgcc tgaaaccatt accgttgccg acttggcaca caaaatggca
     7501 gtcaaaggcg tggaagtggt caaagccctg atgaagatgg gcatgatggt taccatcaac
     7561 caatccatcg accaagacac cgccctgatt gtcgtcgaag aactcggaca catcggcaaa
     7621 cccgccgcag cggacgatcc cgaagccttc ttggacgagg gcgcagaagc agtggaagcc
     7681 gaagcattgc cgcgtccgcc cgttgttacc gtcatgggac acgtcgacca cggcaaaacc
     7741 tcgctgctgg actacatccg ccgcgccaaa gtggtacagg gcgaagcggg cggcattacg
     7801 cagcacatcg gcgcgtacca cgtcaaaacg ccgcgcggcg tgattacctt cttggacacc
     7861 ccgggtcacg aagcctttac cgctatgcgc gcacgcggtg cgaaagcaac cgacatcgtg
     7921 attcttatgg tcgccgccga cgacggcgtg atgccgcaaa ccatcgaagc gattgcccac
     7981 gccaaagcgg caggcgtacc gatggtggtt gccgtcaaca aaatcgataa agaagccgcc
     8041 aacccagagc gtatccgcca agagctgacc gcacacgaag tcgtgcctga cgaatggggc
     8101 ggcgatgtac agtttatcga cgtttccgcg aaaaaaggaa caaacatcga cgcgctgctc
     8161 gaagcagtct tgctcgaagc cgaagtattg gaactgaccg cacctgtcga tgcgcccgcc
     8221 aaaggcatca tcgtcgaggc gcgcttggac aaaggccgcg gcgcggttgc cacattgctg
     8281 gttcaaagcg gtacgctgaa aaaaggcgac atgctgctgg ccggtacggc attcggcaaa
     8341 atccgcgcga tggtcgatga aaacggcaaa tccattaccg aagccggccc gtccatcccc
     8401 gtcgaaatcc tcggcttgtc cgacgtaccg aatgcgggtg aagacgcgat ggtattggcg
     8461 gacgagaaaa aagcgcgcga aatcgccctc ttccgccaag gcaaataccg cgacgtgcgc
     8521 cttgccaaac agcaggcggc gaagctggaa aatatgttca acaatatggg cgaaacccag
     8581 gcccaatctt tgtcggtcat catcaaggca gacgttcagg gctcttacga ggctttggcg
     8641 ggcagcctga aaaaactgtc caccgacgag gtgaaagtga acgtgttgca cagcggcgta
     8701 ggcggcatta ccgaatcgga tgtcaacctt gccatcgctt cgggcgcgtt cattatcggc
     8761 tttaacgtgc gtgcagatgc ctcttcgcgc aaacttgccg aaaatgaaaa cgtggaaatc
     8821 cgctactaca acatcatcta tgatgccatc gacgacgtga aagcggcgat gagcggtatg
     8881 ctttctcccg aagagaaaga gcaagtaacc ggtacggtcg aaatccgtca ggttatctcc
     8941 gtttcaaaag tcggcaatat tgcaggctgt atggttaccg acggcgtggt caaacgcgat
     9001 tcccatgtcc gcctcatccg caacaacgtg gtcatccaca cgggcgaact ggcttcgttg
     9061 aaacgctata aagacgacgt caaagaagtc cgtatgggct ttgagtgcgg cctgatgatt
     9121 aaaggctaca acgaaattat ggaaggcgac caactcgaat gtttcgacat cgtcgaagtg
     9181 gcacgcaccc tgtgattttg atgcggaaaa atgccgtctg aagcgttcag acggcatacg
     9241 aaacgggttc tgtatcatac agaacccgtt ttttgtcgca aatcggcttc agacagccct
     9301 cttgccttat cccgatttga atctgacttg ccatacaaac aggcttcaga cggca
//
LOCUS       DAWKQB010000076         9274 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.076, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000076 DAWKQB010000000
VERSION     DAWKQB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.076"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            162..1505
                     /locus_tag="WJL14_001333"
     CDS             162..1505
                     /locus_tag="WJL14_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ7656394.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAITQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1508..2740
                     /locus_tag="WJL14_001334"
     CDS             1508..2740
                     /locus_tag="WJL14_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ7656395.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALAN
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAITGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2733..3509
                     /locus_tag="WJL14_001335"
     CDS             2733..3509
                     /locus_tag="WJL14_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ7656396.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVKDAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3509..4135
                     /locus_tag="WJL14_001336"
     CDS             3509..4135
                     /locus_tag="WJL14_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ7656397.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4139..4732
                     /gene="nqrE"
                     /locus_tag="WJL14_001337"
     CDS             4139..4732
                     /gene="nqrE"
                     /locus_tag="WJL14_001337"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ7656398.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPANQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYGFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4746..5963
                     /gene="nqrF"
                     /locus_tag="WJL14_001338"
     CDS             4746..5963
                     /gene="nqrF"
                     /locus_tag="WJL14_001338"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ7656399.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6116..7171
                     /locus_tag="WJL14_001339"
     CDS             6116..7171
                     /locus_tag="WJL14_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7656400.1"
                     /translation="MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVHLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            7197..7412
                     /gene="nqrM"
                     /locus_tag="WJL14_001340"
     CDS             7197..7412
                     /gene="nqrM"
                     /locus_tag="WJL14_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ7656401.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7536..8114)
                     /gene="grpE"
                     /locus_tag="WJL14_001341"
     CDS             complement(7536..8114)
                     /gene="grpE"
                     /locus_tag="WJL14_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ7656402.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8298..9116
                     /gene="cysE"
                     /locus_tag="WJL14_001342"
     CDS             8298..9116
                     /gene="cysE"
                     /locus_tag="WJL14_001342"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ7656403.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2235 a         2588 c         2307 g         2144 t
ORIGIN      
        1 cagcttccgc aaggaaaaaa gcagcaaatc tgacaaacag gtacaattcc tgatagaatc
       61 taacactttg ctacattaat ttattcaatg cttttgatgc cacgcgacac ggtatcaaaa
      121 cacgccgttt ttaccttaac catcacaagt aatgcaaata tatgattaaa atcaaaaaag
      181 gtctaaacct gcccatcgcg ggcagaccgg agcaagtcat ttatgacggg cccgtcatta
      241 ccgaagtcgc gttgcttggc gaagaatatg ccggtatgcg cccctcgatg aaagtcaagg
      301 aaggcgatgc cgtcaaaaaa ggccaagtgc tgtttgaaga caaaaagaat ccgggcgtgg
      361 tgtttaccgc gccggtttca ggcaaaatcg ccgccatcca tcgcggcgaa aagcgcgtac
      421 ttcagtcggt cgtgattgcc gttgaaggca acgacgaaat cgagttcgaa cgctacgcgc
      481 ccgaagcgtt ggcaaactta agcggcgaag aagtgcgccg caatctgatc caatccggtt
      541 tgtggactgc gctgcgtacc cgtccgttca gcaaaatccc tgccgtcgat gccgagccgt
      601 tcgccatctt cgtcaatgcg atggacacca atccgctggc ggcagaccct gtggttgtga
      661 tcaaagaagc cgccgaggat ttcagacgag gtttgctggt attgagccgt ttgaccgagc
      721 gtaaaatcca tgtgtgtaag gcagctggcg cagacgtgcc gtctgaaaat gctgccaaca
      781 tcgaaacaca tgaattcggc ggcccgcatc cggccggttt gagtggcacg cacattcatt
      841 tcattgagcc ggtcggtgca aacaaaaccg tttggaccat caattatcaa gatgtaattg
      901 ccatcggacg tttgtttgca acaggccgtc tgaacaccga gcgcgtgatt gctttgggtg
      961 gttctcaagt caacaaacca cgcctcttgc gtaccgtttt gggtgcgaaa gtatcgcaaa
     1021 ttactgcggg cgaattggtt gacgcagaca accgcgtgat ttccggttcg gtattgaacg
     1081 gcgcgattac acaaggcgcg cacgattatt tgggacgcta ccacaatcag atttccgtta
     1141 tcgaagaagg ccgcagcaaa gagctgttcg gctgggttgc gccgcagccg gacaaatact
     1201 ccatcacgcg tacgaccctc ggccatttcc tgaaaaacaa actcttcaag ttcacgacag
     1261 ccgtcaacgg tggcgaccgc gccatggtgc cgattggtac ttacgagcgc gtaatgccgc
     1321 tagacatcct gcctaccctg cttttgcgcg atttaatcgt cggcgatacc gacagcgcgc
     1381 aagcattggg ttgcttggaa ttggacgaag aagacctcgc tttgtgcagc ttcgtctgcc
     1441 cgggcaaata cgaatacggc ccgctgttgc gtaaggtgct ggaaaccatt gagaaggaag
     1501 gctgattatg ggcttgaaac attttctgga aaaaatcgaa ccgcacttcc tgccgggcgg
     1561 caaacatgaa aaatggtatg ccctctacga agctgcggcg acgattttct atacatccgg
     1621 tgcggtaacg cgcaaagcgg cacacgtccg cgatgcgctc gactccaaac gcatgatgat
     1681 tttggtgtgg ctggctttgt tccctgccat gttctacggt atgtacaacg tcggcgcaca
     1741 ggcattcggt gcgttaacgc ccgatttgct gcaacaaagc atcgccaacg actggcatta
     1801 cgcccttgcc aacgctttgg gcatcaatat gtcgtctgaa gcgggcgtgt tgggcaaaat
     1861 gctgttcggc gcgatttact tcctgccgat ttacgcgacc gtatttattg tcggcggttt
     1921 ctgggaagtt ttgttcgcca ccgtgcgcaa acatgaaatc aacgaaggtt tctttgttac
     1981 ctcgattctg tttgccttaa tcgttccgcc cacgctgccg ctgtggcagg cagctttggg
     2041 tatttctttc ggcgttgtgg ttgcgaaaga agtattcggc ggtacaggta aaaacttcat
     2101 gaaccctgcg ctggcaggcc gtgccttcct gttcttcgcc taccctgcca acttgagcgg
     2161 cgatgcggtt tggacggcgg ttgacggcta ttccggcgca accgcgctgg cgcaatgggc
     2221 ggcacacggt gcagacggcc tgaaaaacgc cataaccggt caaaccatca cttggatgga
     2281 tgcgtttatc ggcaaactgc ccggctccat cggcgaagtc tccactttgg cactcttaat
     2341 cggcggcgcg tttatcgtgt ttgcccgcat cgcttcttgg cgcattattg ccggcgtgat
     2401 gatcggtatg attgccatgt cttcgctgtt caacttcatc ggttcggaca ccaacgctat
     2461 gtttgctatg ccttggtact ggcatttggt cgtcggcggc ttcgccatcg gtatgctgtt
     2521 tatggcgacc gaccccgttt ccgcttcctt taccaatgtc ggcaaatggt ggtacggcgc
     2581 actgatcggt gtgatgtgcg tattaatccg cgtggtcaat ccggcttacc ccgaaggcat
     2641 gatgttggcg attctgtttg ccaacctgtt tgccccgatt ttcgactatt tcgtcgcaca
     2701 agcgaacatc aaacgcagaa aggcgcgcag caatggctaa gaaattcgat aaagacagct
     2761 tcagcggcac gctgattgtc gtgttggcgg tcagcctgat ttgctcggtc atcgttgccg
     2821 gtgcggtcgt cggcttgaaa cccatccaag agaaacaaaa actccaagac aaacaaggct
     2881 atatcttgag cgtagccggt ttgatggata aggacaccga catcggtaaa acttttgccg
     2941 agcgtatcga gcaacgcgtg gtcgatttgg cgaccggcga atatgtgaaa gacgcgccga
     3001 aagacttcag tgcgcgcatc gcaggcaaag accccgcgca aagcatccgc atcaaacccg
     3061 aagacgattt ggcaggcatc aaaagccgtg ccaaatacac cgaggtttac ttggtaaaag
     3121 gtgaagacgg caaaatcggc caaatcatcc tgcctatgca cggtaacggt ttgtggtcgg
     3181 tcatgtacgg ctttgtcgcc atccaacccg acggcaacac catcaacggc attacctact
     3241 acgagcaagg cgaaaccccg ggcttaggcg gcgaaatcgg caatcctttg tggcagcaaa
     3301 aattcgtcgg caaaaaactg tttgacggac aaggcaaact cgccctgcac gtcggcaaag
     3361 gcgcgggttc ggacaaagaa cacggcgtag atgccctctc cggcgcgtcg ctgacatcca
     3421 aaggcgtaca aggttcgttc gcctactggt tcggcgaaaa cggctatatc ccctacctga
     3481 acaaattgaa atcagcagga gcgcaataat ggctgatatg aaacgcttga aacatttgat
     3541 gttttcaccc tttatcgaca acaacccgat tgccttgcag gttttgggta tttgttcggc
     3601 tttggcggtt accaccaaac ttcagacggc catcgtgatg ggtatttccg tagctttggt
     3661 aaccggtttt tccagcttct tcatttcgct ggtacgcaac tacatcccca acagcatccg
     3721 catcatcgtg caaatggcga ttatcgcgtc gctggttacg ctggtcgacc aactcttgca
     3781 ggcatttgcc tacgaattgt ccaaacagct ttccgtattc gtcggtctga ttattaccaa
     3841 ctgtatcgtg atgggccgcg ccgaagcatt tgcgatgaaa gagccgccgc tggaaagcct
     3901 gatcgacggc atcggcaacg gcgcgggcta cgggatgatg ctgcttgtcg tcgccaccgt
     3961 ccgcgaactg attggctcgg gcaaactctt gggctacacc gttttccaaa ccgtgcagga
     4021 cggcggctgg tatcagacca acggcttgtt cctgctcgcc cccagcgcgt tcttcatcat
     4081 cggctttttg atttggggac tgcgtacctg gaaacccgaa caggcggagg aataagctat
     4141 ggaacactat ttgagcctct ttatcaaatc cgtcttcatc gaaaatatgg cgctatcctt
     4201 ctttttgggt atgtgtacgt ttttggcggt atccaaaaaa gtatccaccg cattcggttt
     4261 gggtgtggcg gtgattttcg tactcggact gtccgtcccc gccaaccaat tggtttactc
     4321 gctgctcaaa gacggcgcga ttgtcgaagg cgtggatttg acctttttga aattcatcac
     4381 cttcatcggc gtgattgccg ctttggtgca gattttggaa atgttcttgg ataaattcgt
     4441 ccccgccctc tataacgcgc tgggcatcta cctgccgctg attaccgtaa actgcgcgat
     4501 tttcggtgcc gtatcgttta tggcgcagcg cgaatacggc ttcggcgaat ccgtcgtgta
     4561 cggcttcggt gcgggcttgg gctggatgtt ggcgattgtc gctttggcgg gcattaccga
     4621 aaaaatgaaa tattcggacg ctcccaaagg cctcaaaggc ttgggcatta cctttatcgc
     4681 cgccggcctg atggcgatgg cgtttatgtc gttctctggc atccagttat aagaaaggga
     4741 tcggtatgga gattatttta ggtatcgtga tgtttaccgt catcgttttg gttttggcac
     4801 tgatgattct gtttgccaaa tccaaactgg tcagcgaagg cgacatcacc atcaaagtca
     4861 acggcgaaaa agagctgacg atgcccgccg gcggcaaact cttgggcgcg cttgccaacg
     4921 aaggcatctt tatcccctcc gcctgcggcg ggggcggttc gtgcggacaa tgccgcgtcg
     4981 tcgtaaaaag cggcggcggc gacatcctgc cgaccgagct gtcccacatc agcaaacgcg
     5041 aagcacgcga aggctgccgt ctgtcgtgcc aagtcaacgt caaaaccgac atggacatcg
     5101 aagtccccga agaagtgttc ggcgtgaaaa aatgggaatg caccgtcatc tccaacgata
     5161 acaaagccac gttcattaaa gaactcaagc ttgccattcc cgaaggcgaa gaagtcccct
     5221 tccgcgccgg cggctacatt caaattgaag ccccgccgca caccgttgcc tacaaagact
     5281 tcgacattcc cgaggaatac cacgaagact gggacaaata caatttgtgg caatacgttt
     5341 ccaaagtgga cgagccgatt ttgcgtgcct actctatggc ttcgtatcct gaagaaaaag
     5401 gcatcattat gctgaacgtg cgtatcgcca cgccgccccc gcgcgtacct gatgcgcctc
     5461 cgggacaaat gtcgtcctac atctggtcgc tcaaacccgg cgacaaagtt acgatttccg
     5521 gcccgttcgg cgaatttttc gccaaagaca ccgatgccga aatggtattt atcggcggcg
     5581 gtgcgggtat ggctccgatg cgttcccaca ttttcgacca gttgaaacgt ttgaactcca
     5641 aacgtaagat taccttttgg tacggcgcac gttccaaacg cgagatgttc tatgtcgaag
     5701 acttcgacca actcgcagca gaattcccga acttcacgtg gcacgtcgcc ctgtccgacc
     5761 cgctgccgga agacaactgg gacggctaca cgggcttcat ccacaacgtg gtttacgaaa
     5821 accatctgaa aaaccacgaa gcaccggaag actgcgaatt ttatatgtgc ggtccgccaa
     5881 tcatgaacca gtccgtgatc aaaatgctca aagacttggg cgtggaagac gaaaacatcc
     5941 tcttggatga tttcggcggt tgagtattcg gcttcaaata caaaaggaag aaacctcggt
     6001 ttcttccttt ttctatgccg atgccatccg aacacccttc agacggcatc ggcatagaaa
     6061 cataccggcc gttttgcgat ataattcaaa ccatttgcca aaaccgtcaa acactatgcc
     6121 gtctgaaaca cgcctgccga actttatccg caccttgata tttgccctga gttttatctt
     6181 cctgaacgcc tgttcggaac aaaccgcgca aaccgttacc ctgcaaggtg aaacgatggg
     6241 cacgacctat accgtcaaat acctttcaaa taatcgggac aaactcccct cacctgccga
     6301 aatacaaaag cgcatcgatg acgcgcttaa agaagtcaac cggcagatgt ccacctatca
     6361 gcccgactcc gaaatcagcc ggttcaacca acacacagcc ggcaagcccc tccgcatttc
     6421 aagcgacttc gcacacgtta ctgccgaagc cgtccacctg aaccgcctga cacacggcgc
     6481 gctggacgta accgtcggcc ccttggtcaa cctttgggga ttcggccccg acaaatccgt
     6541 tacccgtgaa ccgtcgccgg aacaaatcaa acaagcagca tcttatacgg gcatagacaa
     6601 aatcattttg aaacaaggca aagattacgc ttccttgagc aaaacccacc ccaaggccta
     6661 tttggattta tcttcgattg ccaaaggctt cggcgttgat aaagttgcgg gcgaactgga
     6721 aaaatacggc attcaaaatt atctggtcga aatcggcggc gagttgcacg gcaaaggcaa
     6781 aaacgcgcgc ggcgaacctt ggcgcatcgg catcgaacag cccaacatcg tccaaggcgg
     6841 caatacgcag attatcgtcc cgctgaacaa ccgttcgctt gccacttccg gcgattaccg
     6901 tattttccac gtcgataaaa gcggcaaacg cctctcccat atcattaatc cgaacaacaa
     6961 acgacccatc agccacaacc tcgcctccat cagcgtggtc gcagacagtg cgatgacggc
     7021 ggacggcttg tccacaggat tattcgtatt gggcgaaacc gaagccttaa agctggcaga
     7081 gcgcgaaaaa ctcgctgttt tcctgattgt cagggataaa ggcggctacc gcaccgccat
     7141 gtcttccgaa tttgaaaaac tgctccgcta aacatacaaa ccgattagga aacaccatga
     7201 aaaccctgct ccttaccttc ggcatcttcc tgaccgtcat catcggtatg gcggtcggct
     7261 atattttctc caaacgcagc atcaaaggca gttgcggcgg cattactgcc ttgggtatga
     7321 aaaaaatgtg cgactgcgac acaccttgcg acaccctgca aaagaaactg gatgaagaaa
     7381 aacaggcagg cggcatacgg gttgaccgtt aacgccgaaa caaaatgccg tctgaagccc
     7441 gacaggcggg ttcagacggc attgccgctt tacttcgatc ggtttatacg caaaacgcgc
     7501 ttcaggaaat aaatcagatt attccccaga cgccttcagg cttccttctg tgccaccgta
     7561 accatagccg ggcgcaacac gcggtcggac agcgtataac ccttcttcat cacgcccacc
     7621 acggtattgg gttcctgttc gcttgccacc gcctgcatcg cctgatggat attcggatcg
     7681 agcttatcgc ccgctttagg attgatttcc ttgatttgcg tagcatcaaa tgctttctgc
     7741 aactcgttca aagtcatctg cacgcccatt ttcagcgcat cgaaattgcc gctctgatcc
     7801 aaaagcgcca tttccagata atccttgacc ggcagcattt ccacggcaaa cttctgtccg
     7861 gcgaacttgt gcgtatccgc aatttcctgc tggtggcggc ggcgcaggtt ttgctcgttt
     7921 gccaaagcgc gcagctgctc gtctttcaac tgcgcttcca gctcggcaat ccgcgcctgc
     7981 aaatcctcat aagccggctc tgcggcagcc tgttcctgca caccgtccgc atttcctact
     8041 gtctcgacgg tttccaccgc ctccacattt tcaaccgctt cttcactgtt ttgctgctgt
     8101 gtctgttcgc tcatatcgta tcccttaaaa caaattggaa atcaaaatcc agttattacc
     8161 cgcgcaagat aaggacattt tcaagaaact tcaagcaaag gcagagaatt tcaaattcat
     8221 tttcaaatcc cctaccgaaa aaataatata gacggtaaga tactgccgac catatcgatt
     8281 ttcaaacgtc atcactcatg aaaaaagacc atttaaacac aaccggtttc gacctctggc
     8341 acaccatccg cgaagaaacc gcggccgcgg tctccgccga accgatgctg gcaagttttt
     8401 tgcaccaaac cgtgttgcgc cacgagtccc tcggctccgt ccttgcctac cacctttcca
     8461 gcaaactcgg cagcccgatt atggacgtgc gcgcgctgtt tgaaatctac cagcaggcgt
     8521 tgggcagcga cacccaaatc ggcaaatgcg tcgaagcaga cttaaaagcc atctacgaac
     8581 gcgatcccgc ctgcgacgaa tattcgctgc cgcttttata tttcaaaggc ttccacgcga
     8641 ttcaggcaca ccgcatcaac catcggctgt atctcgacgg gcgcaaaacg ctggcgtatt
     8701 tcctgcaaaa ccgtatgtcc gaagtattcg gcgtggacat ccatcccgcc gcccgtttcg
     8761 gatacgggct gatgctcgac cacgccaccg gctttgttgc cggcgaaacc gccgtgttgg
     8821 gcaacaatat ttcgattttg cacggcgtaa cgctcggcgg ttcgggcaaa gaaggcggcg
     8881 accgccaccc taaaatcggc gacggcgtga tgatcggcgc aaacgcctcg atattgggca
     8941 atatccgcat cggcagcaat gccaaaatcg gggcgggcag cgtcgtggtt tcagacgtgc
     9001 cgccgtccat cacggttgtc ggcgtacccg ccaaacccgt ggcgcgatcg ctcaaaacac
     9061 cgtcggcgga tatggatcaa aatatccagt ttaccgaaat cgactttatg atttgaatat
     9121 ccgcaccaat gccgtctgaa acgccaaacg ggcttcagac gacattgccc gcttcaagcc
     9181 tgcgccttga tatgccgccg atgtcggcac ttgccagtcg gacaaaatgc cttttattcg
     9241 atacacccga ttcccacgca accggaaata tagt
//
LOCUS       DAWKQB010000077         9234 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.077, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000077 DAWKQB010000000
VERSION     DAWKQB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.077"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            89..298
                     /locus_tag="WJL14_001343"
     CDS             89..298
                     /locus_tag="WJL14_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ7656404.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            387..1601
                     /locus_tag="WJL14_001344"
     CDS             387..1601
                     /locus_tag="WJL14_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ7656405.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLDDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIRDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVYQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WJL14_001345"
     CDS             complement(1688..4267)
                     /gene="clpB"
                     /locus_tag="WJL14_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ7656406.1"
                     /translation="MRFDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDIMPSRELQAVLNLTDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDSAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDETVRAVSDAIRRSRSGLADPNKPYGSFLFLGSTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQDNIRNIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4527..5537
                     /gene="trpS"
                     /locus_tag="WJL14_001346"
     CDS             4527..5537
                     /gene="trpS"
                     /locus_tag="WJL14_001346"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ7656407.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNYRFQELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNALTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5543..5884
                     /locus_tag="WJL14_001347"
     CDS             5543..5884
                     /locus_tag="WJL14_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656408.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6203..6526
                     /locus_tag="WJL14_001348"
     CDS             6203..6526
                     /locus_tag="WJL14_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656409.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(6735..8243)
                     /gene="ppx"
                     /locus_tag="WJL14_001349"
     CDS             complement(6735..8243)
                     /gene="ppx"
                     /locus_tag="WJL14_001349"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ7656410.1"
                     /translation="MTTNPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(8359..9090)
                     /locus_tag="WJL14_001350"
     CDS             complement(8359..9090)
                     /locus_tag="WJL14_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7656411.1"
                     /translation="MSREINAGRHHFLYGGISGGGVHYWDNKDFSEQSLRLSFGYKNR
                     SVTRSFGIVPFVEQNLLGGSRYNFVGGFNADFSQRLSERWRLTLNAGNIWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHNITKEAEQASIRKGLRVGAVKTFDG
                     GLGLRANLRYTRRMFDAPGTIVYRFPRKDHEYQANLSLWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         2135 a         2386 c         2432 g         2281 t
ORIGIN      
        1 gatttgaatt tttcagaaaa tgaagcggac gtttgggcgg aggcaacttg tttgataaga
       61 tagcaatatt tcaaaacgga gaaagatcat gccttatgtc aatattaaag taaccggcgg
      121 caaggaagca ccgactgccg cgcaaaaagc ggaactgatc ggcggcgtaa ccgaattgct
      181 ggcacgcgtg ctgggcaaaa atcccgaaac aacggttgtc gtgattgacg aagtggatac
      241 cgataactgg ggaataggcg gcaaaagcgt cagcgaacgg cgcaaagagg gcaggtaaaa
      301 agcctgaaaa tctcggtttg atgctttaaa ttccgcgtga aaaagagtac attcccaccc
      361 attgcccaaa atttacggaa cacatcatgg ataaatttcc caagtctgca aagctcgatc
      421 acgtctgtta cgacatacgc gggccggttc acaaaaaagc ccttcagttg gaagaggagg
      481 gcaataaaat ccttaaactc aatatcggca accctgcgcc gttcggcttt gaagcccctg
      541 atgaaatctt ggtcgatgtc atccgcaacc tgccgacttc gcaaggctat tgcgattcta
      601 aagggctgta ttccgcccgc aaagccattg ttcactacta tcagaccaaa ggtttgcgcg
      661 atattacggt tgatgatgtc tatatcggca acggcgtgtc cgagctgatt acgatgtcta
      721 tgcaggcatt gctcgacgac ggcgacgaaa tcctgattcc cgcgcccgac taccctttgt
      781 ggacggcggc ggcaacgctt gcgggcggta cggtacgcca ttatctgtgc gacgaagaaa
      841 acggctggtt ccccaacctt gccgatatgg aagccaaaat cacgcccaaa accaaagcca
      901 tcgtcgtcat caatcccaat aatccgacag gcgcggtgta cagcagggaa atcctgttgg
      961 aaatcgccga actggcgcgc aagcacggtt tgattatttt cgccgacgag atttacgata
     1021 aaatccttta tgacggagcg gttcaccacc acatcgccgc gcttgccccc gaccttttga
     1081 cggtaacgtt caacggtttg tccaaatcct accgcgtcgc cggcttccgc caaggctgga
     1141 tggtgctcaa cgggccgaaa catcacgcaa aaggttacat cgagggtttg gatatgctct
     1201 cgtccatgcg cttgtgtgcc aatacgccga tgcagcacgc gattcagacg gcattgggcg
     1261 gctatcagag cattaacgaa tttatcctgc cgggcgggcg gcttttggaa cagcgcaaca
     1321 gggcgtggga actggtcagc caaatccccg gcgtgtcctg cgtcaaaccg atgggtgcga
     1381 tgtatatgtt cccgaaaatc gataccgaaa tgtaccgcat ccgcgatgac atgaaattcg
     1441 tttacgattt gctggtgcgc gaaaaagtct tgctggtgca gggaacgggg tttaattgga
     1501 tcaagcccga ccatttccgc attgttacgc tgccttacgt ctatcagatt gaagaggcga
     1561 tgggcaggct ggcaagattc ctgcaaacct accgccaata aggggacggt ttgtctgccg
     1621 aggataaaaa atgccgtctg aaacggagat tcccgtttca gacggcattt tcaacagcag
     1681 gaacgaatca ggcaaatttc agtctgtcgc cgtcggcttc caccctgatt tcgctttcgg
     1741 gcgcatagtt tccggcaagc agggcttttg ccagcgggtt ttcgatttcc gactggatgg
     1801 cgcgtttgag cggacgtgcg ccgtaaatcg ggtcgaaacc ggctttggcg atgatgtcca
     1861 gagcggcatc ggaaacagtc aggcggaggt tttgtttttc caaacgtttt tccaagcctt
     1921 tgagctggat tttcgcaatg ttgcggatat tatcctgatc cagtccgtgg aacacgacca
     1981 cttcgtcgat gcggttgatc atttcaggac ggaaatgttc tttcacatcc tccatcacaa
     2041 cttcttttac cgcttcgtaa tcctgaatgc ccatttgttg gatgtgctgg ctgccgatgt
     2101 tggaagtcat cacaatcacg gtatttttaa aatctaccgt cctgccttgc ccgtcggtca
     2161 aacgaccgtc atccaatact tgcagcagga tgttgaacac gtcgggatgg gctttttcca
     2221 cttcgtccag cagaatcacg ctgtacggtt tacggcggac ttgttcggtc aggtaaccgc
     2281 cttcttcgta gccgacatag cctggaggcg cgccgattaa gcgggcaacg gcgtgttttt
     2341 ccatatattc ggacatatcg atacggatga ggtggtcttc gctgtcaaac aggaaacctg
     2401 ccaatgcctt acacaattcg gtcttaccca cgccggtcga gcccaagaac aggaagctgc
     2461 cgtagggttt attcgggtcg gcaagaccgg aacggctgcg gcggatagcg tcggacacgg
     2521 cacgcacggt ttcgtcttga ccgactacgc ggcggtgcaa cacttcttcc attttcagca
     2581 acttgtcgcg ttcgccttcc atcatttttg acacaggaat gccggtcata cgggaaacaa
     2641 cttctgcgat ttcctctgcg ccgacattat tacgcaagag tttgtttgcc ggttttgtgc
     2701 tgtccgtatc tgcccgttcg gcggctgcac gctgtttttc caaatgctcc aaatcttcat
     2761 acatcaattt tgaagccagt gccaaatcgc cttgccgttt tgcctgttcg attttaattt
     2821 tgacttcgtc aatttgtttc ttaatattag cagcaccgtc tgaaattgct ttttcggctt
     2881 tccagatttc gtctaaatcg gcgtattctt tttgcagacc gttgatttcc tcgtctatca
     2941 gttccaaacg ttttttgctg gcatcgtctt tttctttttc aacgtgcgcc ttttccatcc
     3001 gaagctgaat tagacggcgg tcgattttgt ccattgcttc cggcttggtt tctttttcca
     3061 tcttgacacg gctggcggct tcgtcaatca aatcaatcgc tttatcaggc aggaagcggt
     3121 cggtaatgta gcggtcgctc aactccgctg cggcaacgat agcagggtcg gtaatatcga
     3181 taccgtggtg gatttcataa cgctcctgta aaccgcgcaa gatagcgatg gtgtcttcta
     3241 cacttggctc gccgaccaat actttttgga agcggcgttc gagtgccgcg tctttttcga
     3301 tgtattggcg gtattcgtcc aaggtggtcg cgccgataca gtgcaattcg ccacgtgcca
     3361 aagccggttt cagcatattg cccgcatcca tcgcactgtc ggttttgcct gcgccgacca
     3421 aagtatggat ttcatcaatg aaaatcagag tgttgccgtc gtctttcgcc aaatcgttca
     3481 atacgccttt caagcgttct tcaaattcgc cgcggtattt cgcgccggca atcaaagccg
     3541 ccaaatccaa aaccagcagg cgtttgttac gcagggattc aggtacttcg ccattgacga
     3601 tacgttgcgc caagccttca acaatagcgg ttttacccac acccggctca ccaatcagca
     3661 cagggttgtt tttggtgcgg cgttgcagca cttgaatcgc gcggcggatt tcgtcgtcac
     3721 gaccgataac ggggtcgagc ttaccgtctc gggcgcgctg ggtcaggtcg agcgtgtatt
     3781 ttttcagcgc atcgcgttgg tcttcggcat tggcatcgtt cacgttttgt cctcctcgta
     3841 ccgcgtcaat cgcggcattg atgttttgtt cggtcgcgcc ggcttctttc aaaattttgc
     3901 cggccgcatc gttctgctgc accaaggcaa gcaggaaaag ttcactggcg atataggcat
     3961 cgccgcgttt ggttgcggct ttgtccgtca ggttcaacac cgcctgcaat tcacggctgg
     4021 gcataatgtc gccgccctga ccggacactt tcggcaagct gtttaaatgc tgctgcaaac
     4081 gctgtttcac ttgcggcacg ttcacgcccg catgagccaa gagcgcggca gcaccgctgt
     4141 tttggtcgtc aagcagggct ttcagtacaa agcccgcttc cagatagctg ctgtccgcag
     4201 ccaacgccaa actctgagct tctgcaaggg cttgttggaa tttggcggtt aatttgtcga
     4261 atcgcatttt tgtttccttt tcaaaatgtc cgctgtcgaa gcctatatgt gcatcattgt
     4321 ggataactca agttctgttt tctgtttttc tatatttaat tcgatatatc attgaattta
     4381 aagtatataa aaatgtataa taatgtgtat aactatatct tcttaatatg gaaaagtctg
     4441 ttgtcggctg gatgtaggtg gcaaatcggg tataatcggc gcatcttttt ccctttcaga
     4501 cggcattgat gccgtaagga cattttatga gcaaaaaacg agttctgacc ggcgtaacca
     4561 ccaccggcat cccgcatctg ggcaactacg tcggcgccat ccgccccgcc gtccgcgcgg
     4621 cgcaaaaccc cgataccgaa tccttcctct tcctcgccga ttaccacggc atcatcaaat
     4681 gccacgagcc ggagatgatt caccaatcca cccaagccgt tgccgccact tggcttgcct
     4741 gcggactcga ccccgagcgc accaccttct accgccaaag cgacattccc gaagtgatgg
     4801 aattgaactg gattctgacc tgcatcaccg ccaagggttt gatgaaccgc gcccatgcct
     4861 acaaagccgc cgtgcaggca aatgcagaaa acaatcagga agatcctgat ttcggtgtgg
     4921 aaatgggttt gttcagttat ccgattctga tgactgccga catccttatg ttcaacgcca
     4981 acgaagttcc tgtcgggcgc gaccaaatcc aacacgtcga aatggcgcgc gacatcgccg
     5041 gccgcttcaa ctaccgtttc caagaactct tcaccctgcc cgaagtgaaa atcgacgaaa
     5101 acgtcgaact tttggtcggc ttggacggac gcaaaatgtc caaatcttac ggcaacacca
     5161 ttccgctttg ggaaaacgac aagaaaaccc aaaaatcggt caacaaaatc atcaccaata
     5221 tgaaagagcc gggcgagccg aaacagcccg atgaaagccc attgtttgaa atctacaaag
     5281 ccttctccac accgtctgaa acggtggaat ttacgaaaat gcttgccgac ggcttggctt
     5341 ggggcgaggc aaaaaaactt ttggcggcga aaatcaacgc cgaactcgcc gaaccgcgcg
     5401 aacgctacaa cgcattgacc gccgaccctt cgcaaatcga agagattttg caggcaggcg
     5461 cgcaaaaagc gcgtaaagaa gcacgcgaat tgttggacaa agtacgcgat gcggtcggca
     5521 tccgcccgtt gaaataaacc cgatgccgtc cgaacctctt aaaggcttgc cggaacaaaa
     5581 cgtcgtccgg ctgaccggca agcatcccaa cgacttggaa gccgtcagcg gcaaatgcat
     5641 ggaaaccgac gataagggcg gtccggtagg ctgggcggaa aacggcgtgt gccatacctt
     5701 gtttgccaaa ctggtgggca atatcgccga agacggcggc aaactgacgg attacctgat
     5761 ttcgcattcc gccctgcaac cctatcaggc aggcaaaagc ggctatgccg ccgtgcagaa
     5821 cggacgctat gtgctggaaa tcgacagcga gggggcgttt tatttccgcc gccgccatta
     5881 ttgaggtatt cggacatccc ggaatatatt tgggtttttc aaaccctgca ggaaaaagtc
     5941 cgcaccgtcg ggaaactcaa aaggaggggg atatgtgtta caattttccg aactgttttc
     6001 ataaaatagt tttcggacgt gtttcaatat ggcattgatg ccgccttatt gttcgagaaa
     6061 aaacctttat atttaaatat aatgggtttt aactaaacgg gaaaccgttt tctctccggc
     6121 cgatgggcaa aatcagccga ttgatggaac acggtccgat ttttaagcaa aaccttaatc
     6181 aacaactcaa gaaggataag acatgaaaaa attattgatt gccgcaatga tggcggctgc
     6241 cttggcagct tgttcgcaag aagccaaaca ggaggttaag gaagcggttc aagccgttga
     6301 gtccgatgtt aaagacactg cggcttctgc cgccgagtct gccgcttctg ccgtcgaaga
     6361 agcgaaagac caagtcaaag atgctgcggc tgatgcaaag gcaagtgccg aggaagctgt
     6421 aactgaagcc aaagaagctg taactgaagc agctaaagat actttgaaca aagctgccga
     6481 cgcgactcag gaagcggcag acaaaatgaa agatgccgcc aaataatttg ttgccttggc
     6541 aaaaatgatg ggatgccgcc tgccggcaac ccaaacgaaa ccgcctgaag attttcaggc
     6601 ggtttttgag ttgtatgccc cttgtttttt acgcctgcat gaccgttccg agcagatgtg
     6661 ccgcgcatat cgggatgttg cggacaggca aatgccgtct ggacagggtt tggacggcat
     6721 ttgttccgca aggttcagac ggcctcgact ttaaacggca tattgatttt ttgccattgg
     6781 acgctttcat attccaatgc gtcggcaatc aggggatggc gttccagcca ttccctgtca
     6841 atacgcagga tgaagccgca gctttccgta tccgtgcgca actgcatatt tttcgggaaa
     6901 gacaggtctt ggcgcgaacg gcagaacagg gcagcaaggc gcagggacaa aacggcatac
     6961 cacaacattt cgttggtgcc gatgatgccg ctcatttttt tcatatcgcc gcgatgaccg
     7021 atgaccagtt gggcaagtat ggtctgttct ttgcgtgaga aacccggcat atcggcgttt
     7081 tcgaggatgt aggcggaatg cttgtgatag ccggtgtggg cgatgtccaa accgatttcg
     7141 tgcagcgcgg cggcgcgtcc gagatactgt tgccacaagg caagctcttg aactgtaacg
     7201 tttttagcgt ggcagaggct gtccataaag gtttgcgcgg tctcggcggt gcgtttcgcc
     7261 tgattgaggc tgacgtggta gcggtgttgg aactcggcaa ccgtttgtcc gcgcatatct
     7321 tcgtttaaac cgcgcccgat caaatcgtaa aacacgccgt cgcgcagggc ggcttcggtt
     7381 acggtcatcc tgtcgagttt catttcctca aacgccgcca tcatcacggc aagtccgccg
     7441 gcaaaaactt cgatgcgttc cggtttcagg ttttcaaatt tggctttttt gaccgaaccg
     7501 gcttcgatga tgcgttcggc gagggcgcgc atgcctttgt aggtaatgtc cgcctcttgg
     7561 ggcatttcgg cggcaagcac gtcgcggatg gattttgccg aacccgatgt gccgacggcg
     7621 aaatcccaac cttcgcgcct catatttttg ctgatgcgct ggatttcgtt gcgggcggcg
     7681 gaaatggcag attggaagtc tttggcggtg attttgtttt ggaagaagcg caggctgtag
     7741 gtaacgcagc ccaagggcag gctttcggta atgtcgggat tcagcgtcga accgatgaca
     7801 aattctgtcg aaccgccgcc gatgtcgata accagcattt tgccgccgcc cggggggagg
     7861 gtgtggatca cgccggtata aatcagccgc gcctcttcgc gcccggcgat gatttcgatg
     7921 gggaaaccca gtgccgcttc ggctttggga aggaaatctg cgatgttttt ggcgacgcgg
     7981 aatgtattgg ttgccacggc gcgtacctgt tcagggcgga agccgcgcag gcgttcgccg
     8041 aattttgcca gacagtccaa agcctgttct tgggaagcgg cactcaggtt tttctgttcg
     8101 tccagtccgg cggcgaagcg caccatctgt ttgaacgaat cgatgacttt taattgtccg
     8161 ttgttgtttt cgcaaatctg gaggcggaaa ctgttagaac ccaaatcgac ggaggcaaga
     8221 acgtttgcgg gattggtggt catggcggat accggtgggg gaaaaacgca atgttactct
     8281 gacggcgcag gcgttgacaa taaatgatgc ggcggttttt gattctgccc acggatgttg
     8341 ccgacggcat ttttgcgctt atttgaaatc cttttccacg ctcatgaaaa tctgcgtgtt
     8401 tttgcgtgtg taaaaacttt tcatattgct gtcgattttc aggtagcgga aattgagttg
     8461 cggcgtaaag cccttccaag agattttgtc gtgccacaac gacaggtttg cctgatattc
     8521 gtggtctttg cgcgggaagc ggtacacaat ggtcccgggt gcgtcaaaca tcctgcgggt
     8581 gtagcgcagg tttgcccgca gacccaagcc gccgtcgaac gttttgaccg cgccgacacg
     8641 caaacccttg cggatggaag cctgttccgc ctctttcgtt atgttgtgcg accagtccgc
     8701 accgccgtaa agcagccagt ctttcggcgc ggaatacatc agcgtcgcgc ccgccagcgg
     8761 catatggctg tcgtatcggg cggcggtgcg gtcttcctga taatgcttcc atatattgcc
     8821 cgcgtttagt gtcaaccgcc agcgttcgct caagcgttgg gagaaatcgg cattgaagcc
     8881 gccgacgaaa ttgtatcggc tgccgcctaa gaggttttgc tcgacaaacg gcacgatgcc
     8941 gaacgagcgc gttaccgaac ggtttttata gccgaacgac aggcgcaggc tctgttcgct
     9001 gaaatctttg ttatcccaat aatgcacgcc gccgccgctg atgccgccgt agaggaaatg
     9061 atgcctgccc gcattgattt cgcgcgacac gcctaagccc tagcgcaaac cgtgtgcctt
     9121 ttgcggcagg ctgtcggcgg ttttcgtcca gcttctgccc gcaaattcaa tcgttttttc
     9181 ggacgaagcg ttgtttatag cggattaaca aaaatcagga caaggcgacg aagc
//
LOCUS       DAWKQB010000078         9122 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.078, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000078 DAWKQB010000000
VERSION     DAWKQB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9122
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.078"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(59..1315)
                     /locus_tag="WJL14_001351"
     CDS             complement(59..1315)
                     /locus_tag="WJL14_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ7656412.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNV
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVAKLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     HTGKYLRLNSGEHQTDGFFAAVLQRK"
     gene            complement(1663..2004)
                     /locus_tag="WJL14_001352"
     CDS             complement(1663..2004)
                     /locus_tag="WJL14_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656413.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEVDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2208..3065)
                     /locus_tag="WJL14_001353"
     CDS             complement(2208..3065)
                     /locus_tag="WJL14_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ7656414.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADSAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVARLNGKPYHIELTCKYYGGGTGRPEDMHGLDPTDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3126..3941)
                     /locus_tag="WJL14_001354"
     CDS             complement(3126..3941)
                     /locus_tag="WJL14_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ7656415.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4185..4970
                     /locus_tag="WJL14_001355"
     CDS             4185..4970
                     /locus_tag="WJL14_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ7656416.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLVILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMSGEDGWLESGDIVAANPKVLAQMLKIISAHV"
     gene            5229..7349
                     /locus_tag="WJL14_001356"
     CDS             5229..7349
                     /locus_tag="WJL14_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7656417.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDSRQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMDP
                     EGAVSGSVNIETKKAADEGNRKIGLGWFSNNRAQGTFDLGQRFGENKAFGVRANGKLR
                     HGDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTH
                     NWSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFKADYPNSWAKTANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTDKKKSQSGDAKRFSPMLMAAWVSQP
                     DLVVYGNYMEDLERADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKR
                     PGYWRGQTTTNAKTKKKTLTYGDNSDFARYKAQGGAAGDEQGMERSRGIEFNTYANLL
                     NNTLRPTLGLMYLQSTVKNYPNAADNLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNG
                     NVSYFGKSYQDTKKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQR
                     GQFDRSFAVVGMPRTYWLKAELDF"
     gene            7474..9024
                     /locus_tag="WJL14_001357"
     CDS             7474..9024
                     /locus_tag="WJL14_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ7656418.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLHNAQKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSAKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     ARISAKKMTEEQIRNDLIAAVKGEASGLFTHNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2274 a         2156 c         2424 g         2268 t
ORIGIN      
        1 atgaaatgcc gtctgaacgc tttgaaaagg gttcagacgg cattttgttc aaaccggttt
       61 atttgcgttg caatacggcg gcgaagaagc cgtcggtttg gtgttctcct gagttgaggc
      121 gcaggtattt tccggtatgc aaatcgactt tcaggctttg cagcagttcg gcacagttga
      181 cgggttcaaa ttcgggatgt tcggacagga aacgttcgac ttgcagctcg ttttcttcgg
      241 gcaggatgct gcaagtggcg tacaccaagc ggccttgcgg ttttaccagt ttggaggcgg
      301 catcgaggat gctgtgttgc tgttccaaaa gtttggcgac ggtttcggcg gattggcggt
      361 atttgagatc gggattacgg cgtaaagtac ccaaaccgga gcagggcgca tccactaaaa
      421 cgcggtcggc tttgcctgcc agtcgtgcga tacggctatc atgttcgctg ctgatgcgtt
      481 cggggtggac gttggtcagt ccggcgcggg tcatgcgcgg tttgaggttg gcaaggcgtt
      541 tttcggcgat gtcgaaggcg tagattctgc ctttgttcgc catttgcgcg ccgatggcga
      601 gggttttgcc gcctgcgccg gcacagaaat cgacaatgat ttcgccgcgt tttgcgccca
      661 ccaataaagc aagcagctgg ctgccttcgt cttggacttc cagtgtgccg tccaaaaaca
      721 gttcgtgttt gttaagcgcg attttgtttt tcaggcggat gccccaaggc gaataaggcg
      781 ttgcctccgc atcggcactt tcggcttgca acaatggcag cactttatcg cgtttgcctt
      841 tcaaggtatt gacgcggatg tcgagcggcg cggcttggtt gatgctgcgt ccgaaggcga
      901 ggatttcttc ttcgttccaa tgctgttgca gttgttcgac cagccattgc ggcaattctg
      961 cggcggtatt cagaccgtct gaaaactcgg ttttgcgggc tttcaaattg ccgaggaacg
     1021 ctgtttcttc ttcatcaagc aggtctttga tttggctgat gttggtgctt ctgccgagaa
     1081 ccagtgcggc gagtgcggct ttgcgtggct gcgcgtgagg acggcgcagg gcagtgctga
     1141 ttttttgata gtggcgcagc gcggcaaagg cggtttcggc gatttcgtgg cggtcttggc
     1201 ggccgagctt tttatgttcg cggaagtagg cggagaggac ggcatcggaa ggctgtttga
     1261 aagtcagcat ttcagccaaa actttggcgg tatggtcgag ttgtgcggcg ttcataatgt
     1321 aatgggttct cttactgtgg gttattcata atcaatctaa cgtccttaaa ataacaatag
     1381 catctttatc attacttagt attctatccg caataagaaa gatgtgttaa aagtgcgcaa
     1441 tcgggatttg tctaaatttc tccgttaact acaaaggcag cctgaaaaaa gttctatttt
     1501 caggtcgctt gaacagcatc cgatataatc acatcagcga tttcattttg taatcactcc
     1561 ccaaaacatg aggaaaaaaa caaaatttat tttcttcgtt cagcatatct atcctaaata
     1621 tgccatcact ccattgagtt tttaatttgt atttcgctct tgttaaatgt ttttctgttt
     1681 gccgatttct tttgctaatg taaaaaatac tctaccactc tcattggtta aagttttcaa
     1741 gtattcatca acatcagtaa cattaataac aactgtttca tttgtatgtt tattaaattc
     1801 taaatcaata ataatattcc cattaaccaa tatttcatat atatctactt cttcaggcag
     1861 catagacaaa caataagcac ttgaagctga aaactctttt ttcagatact gaagaagaaa
     1921 agtattttta attaaaatat tattaaattt atctaatgat tgcttgactt ctttagttgt
     1981 ccaattttta acattattct tcattgtatt ctacctgggg tcgtagttat taaattacca
     2041 tacttatttg tttggatcgt cataatatta gttggcatgt tgttgttata tttatcatat
     2101 ccaatatcct tattaactcg gatgactctt tcataaattg tatgggaaat gagatttcca
     2161 tcactaagcc tctcaataga tatacgcgtt aatgttggga aagggtttca gacggccttc
     2221 ataatgcgtt cgatttcgtg ccaaaagccg tccggccgac attccaaaac ggcaatcaag
     2281 ccttggtcga tacggcggat ttcggtttcg ttcaatgttt cggtttcggc ggcacgcgca
     2341 ggtgcgaggt aggccatgcg gtcgagcaga tggtagcgga cttcttggcg ttcaaacccg
     2401 tattccgccc agtcttgaat atagatgccg cgccagccgt atgggttaag cggcggctca
     2461 aaagcatgga aactttgtgg aaaaaatccg atgccgcgca cgatggaaac gggttttacg
     2521 tcaagcggca gaccgtgttg ccgcaaggtt cggatgccgt ctgaagtgtg cggcagaccg
     2581 agttgggcgg tcagtttggc ggcttttccc aacagcgtgt ctgtggggtc gagtccgtgc
     2641 atgtcttcgg gtctgcccgt gccgccgccg taatatttgc aggtcagctc gatatggtag
     2701 ggtttgccgt taagccttgc cacaaaatcc gccgcgcctt gcgtattgcc gtccgaaccg
     2761 gaaaccgtga ggttgtgcgc gagcagttcg gcgtgcggcg cgttggcaaa ccaaaatgcc
     2821 agcagttctt cagcgtaaat gcccaagcgg tgtccgaacg gggcgcgtcg ggtgaggtgt
     2881 tccgtcagcg gcgcggaatc ggcatccaat gccaaaaggt aacggaaacc gcgttccccc
     2941 aatagttcgc gcacgcccaa ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc
     3001 gaggcgaggt cgcggacggg ttggctggtg agcttccacc ataatgcgtc tagggcgtaa
     3061 ttcatgctta tccgaatatc agtcatgccg tctgaaaagc tggaaatgtt ttcagacggc
     3121 cttaatcagt cttttttatg gatacgatgg ctgacggtat tgaaaaaacc gcgcaggata
     3181 tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg gcgcatcaga
     3241 cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc catgtgggcg
     3301 accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag atgggtcatg
     3361 ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg ggcgaggttg
     3421 agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc ttggacttct
     3481 tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc gcctcggttt
     3541 gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc agtaatttcg
     3601 cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc gtccaaagaa
     3661 gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat gaagctttct
     3721 tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt ttccgtcatc
     3781 ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat tgttttcatc
     3841 gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat gatgcggatg
     3901 ttgtccagat aagcgggcag ggcgggtttg agagtagtca tgttcggttt tcggtcggaa
     3961 tggaataggg tataatgcgc cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt
     4021 tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc aggcggtatt
     4081 ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc gctccgaagg
     4141 actgtacgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat ccgtttttga
     4201 atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc gcgggcaatc
     4261 tcgatgccgt caaaaccgac agcaaagcct tcaacgattt tgtttccgat gttgaccgca
     4321 attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac aaaatcactt
     4381 gtgaagaaag cggctcccac ggcaaagccg cagccgagta cgaatggatt atcgatccgc
     4441 tcgacggcac gaccaatttc cttcacggtc atccccaata cgccatctct atggcgctgc
     4501 tgcacaaagg cgtgttgcaa gaagctttgg tttacgcccc cgaacgcaac gacgtatata
     4561 tggcttcgcg cggcaaaggc gcgttgctca acgaccgccg catccgcgtt tccaaccgca
     4621 tcgaattgaa ccgctgcctg atcggtaccg gcttccctgt tgtcgatcaa agcatgatgg
     4681 acaagtatct ggtgattttg aaagatttct tggccaaaac cgccggcggc cgtcgtgaag
     4741 gcgcggcttc tttggatttg tgtgctgtag caacaggtcg ttttgacggc tttttcgagt
     4801 ttaacctcaa accgtgggac attgccgcag gtgcattgat tgttcaggaa gcgggcggta
     4861 tcgtaaccga tatgtcgggt gaagacggct ggctggaaag cggcgatatt gtggctgcca
     4921 atcccaaagt gctggcgcaa atgttgaaaa tcatttccgc acacgtttaa atttcagccc
     4981 gctcccggga atgcgggaaa acgggcgggt atgccgtctg aagtatcgcg ccgatatttc
     5041 agacggcatt tttactggca gcgggggcag gaaggtggaa acggctaaat gcccgaaact
     5101 gccgaggatg gaaactaaac ggagaaccct gtgcaatttg tttatagtgg attaacaaaa
     5161 accagtacgg tgttgcctcg ccttagctca aagagaacga ttctctaagg tgctgaagca
     5221 ccaagtgaat gggcaaagcc aacgccttta ccgcgccgat taccgtcgtc aattacgacg
     5281 aacaagccct aaacaacacc gaagcgcgta ctttggtgga tgccgtagcg aaaaaagacg
     5341 cttccgtttg gcagttcggc ggcgaaagca acacgctgac cggactgtat ttcagaggtt
     5401 atcagcttga ctcgcgccaa ttcagcgtca acggtttggc aggtatgtac ggcacgcaag
     5461 gcacggccag cgtgcaagtt ggatcggcac aactgattaa aggcgcatcc actgccgtaa
     5521 acggcatgga ccctgaaggt gcggtatccg gttccgtcaa tatcgaaacc aaaaaagccg
     5581 ccgatgaagg caaccgcaaa atcggtttgg gctggttcag taacaaccgc gcccaaggta
     5641 cgttcgactt gggccaacgt ttcggcgaaa acaaagcatt cggcgtgcgc gccaacggca
     5701 aactgcgcca cggcgacacc ccgcgccacg gttacagcga agacaacaaa gaatttgcgg
     5761 taaatgccga ctatcgcggc gaaacactgc gcgtagcgtt cgattccatc tacgcaaaac
     5821 gcaaaaccaa cggcggtcgc gcgcgtatgc aggacattca aaacgccggc ggacgcttgt
     5881 ttggcgcgcc cgatggtaaa accaacctgc tgccttcttg gaactggcaa aataccgccg
     5941 gccaaaccaa tatgcttacg tttgaatggg atgcgtttga taatgcacag attacaggcg
     6001 gtatcggcta caacaaggca cgctattacg gcacgctgat ttccccgacc gtatgtttga
     6061 atgctaccag cacttgtacc gatatgcaaa acacaaacgc cagcggcaaa gtaacggcac
     6121 gcaactatga ctacaatacc ggtactgcgc gcctgaccga ccaatacttc cgcactttaa
     6181 gcatgaacct gtctgcgcga ggcgaatttg aaaccggccc ggtaacacat aactggagta
     6241 cggctttcga ccgcgttatc cgccagcgga aaaccataaa cggcagcggg aacggcaaca
     6301 gcaagataga agtgaaggca aacgaaaata ttgcgaatca attggcatcg ttcaaagccg
     6361 actatcccaa ttcttgggcg aaaaccgcca acttggatgc caatatcaaa gttaacagcc
     6421 tggctttgtc cgacaccttg ggcttcgttg ataacaaata ccgcctgact ttgggcggac
     6481 gtttccaagc ggttgaatac actgataaga aaaaatcaca gagcggcgat gccaaacgct
     6541 tcagtccgat gctgatggcg gcgtgggtgt cgcaacctga tttggttgtg tacggcaact
     6601 atatggaaga cttggagcgt gccgacatca aaaccgatga ttccggcgaa acgacaatgg
     6661 cgaaaccgcg tgtcagccgt cagtttgaag tgggcgtgcg taaaaactgg ggcaatttcg
     6721 taaccacgtt aaacgcgttc caaatcaaac gcccgggcta ctggagaggt cagacaacca
     6781 ctaatgcaaa aactaagaaa aaaacgctga cttacggcga taattccgat tttgcccgct
     6841 ataaagccca aggaggcgca gccggcgacg agcaaggtat ggagcggagc cgaggtattg
     6901 agttcaacac ctacgcgaat ttgctcaaca atactttgcg tccgactttg ggtctgatgt
     6961 atctgcaatc gaccgtgaag aattacccga acgcagccga taatcttgtt aacggcgtac
     7021 aagtcgccaa tccacgcgta attgccaagg caggtttgga atgggataca ccgtttgcca
     7081 aaggcttgac tttaaacggc aacgtttcgt atttcggcaa gtcttaccaa gatacgaaaa
     7141 aacaatacgc cttcccgtct tacactttgg ttgacgtagg cgcgcgctac aaaaccaagc
     7201 ttggcaaaaa cactctgacc gtcagcagct cggtagaaaa cctgtttaac aaaaactact
     7261 ggcaggtaca gcgcggacaa tttgaccgta gcttcgccgt cgtcggtatg ccgcgtactt
     7321 actggctgaa agcggaattg gatttctaag ctgcctccgt ttccgaaaat gccgtccgaa
     7381 caggtttcag acggcatttc ttttaaagaa atatggcggg atacgcttaa ataatgctat
     7441 tctagcgtct ttgaaacctg cttgtttgac gtgatgaaaa aacctttgat ttcggttgcg
     7501 gcagcattgc tcggcgttgc tttgggcacg ccttattatt tgggtgtcaa agccgaagaa
     7561 agcttgacgc agcagcaaaa aatattgcag gaagcgggct tcttgaccgt cgaatcgcac
     7621 caatatgagc gcggctggtt tacctctacg gaaacgacgg tcatccgctt gaaacccgag
     7681 ttgctgcata atgcgcagaa atacctgccg gataacctga aaacagtgtt ggaacagccg
     7741 gttacgctgg taaaccatat cacgcacggt ccttttgccg gcggattcgg cacgcaggcg
     7801 tacattgaaa ccgagttcaa atacgcgcct gaaacggaaa aagttctgga acgctttttt
     7861 ggaaaacaag tcccggtttc ccttgccaat accgtttatt ttaacggcag cggtaaaatg
     7921 gaagtcagtg ttcccgcctt cgattatgaa gagctgtcgg gcatcaggct gcactgggaa
     7981 ggcctgacgg gagaaacggt ttatcaaaaa ggtttcaaaa gctaccggaa cggctatgat
     8041 gcccccttgt ttaaaatcaa gctggcagac aaaggcgatg ccgcgtttga aaaagtgcat
     8101 ttcgattcgg aaacttcaga cggcatcaac ccgcttgctt tgggcagcag caatctgacc
     8161 ttggaaaaat tttccttaga atggaaagag ggtgtcgatt acaacgtcaa gttaaacgaa
     8221 ctggtcaatc ttgttaccga tttgcagatt ggcgcgttta tcaatcccaa cggcagcatc
     8281 gcaccttcca aaatcgaagt cggcaagctg gctttttcag ccaagaccgg ggaatcgggc
     8341 gcgtttatcg atagcgaagg gcagttccgt tttggcacgc tggtttacgg cgatgaaaaa
     8401 tacggccctc tggacatcca tatcgctgcc gaacacctcg atgcttctgc cttaaccgta
     8461 ttgaaacgca agtttgcacg aatttctgcc aaaaaaatga ctgaagaaca aatccgcaat
     8521 gatttgattg cggcagtcaa aggcgaggct tccggattat ttacccataa cccagtattg
     8581 gacattaaaa ctttccgatt cacgctgcca tcgggaaaaa tcgatgtggg cggaaaaatc
     8641 atgtttaaag acatgaagaa ggaagatttg aaccaattgg gtttgatgct gaagaaaacc
     8701 gaagccgaca tcagaatgag tattccccaa aaaatgctgg aagacttggc ggtcagtcaa
     8761 gcaggcaata ttttcagcgt caatgccgaa gatgaggcgg aaggcagggc aagtcttgac
     8821 gacatcaacg agaccttgcg cctgatggtg gacagtacgg ttcagagtat ggcaagggaa
     8881 aaatatctga ctttgaacgg cgaccagatt gatactgcca tttctctgaa aaacaatcag
     8941 ttgaaattga acggtaaaac gttgcaaaac gaaccggagc cggattttga tgaaggcggt
     9001 atggtttcag agccgcagca gtaatgggtc aaatgccgtc tgaaatgttc aggcggcatt
     9061 ttgttgtatt ggaaactgcc gttttcaacc attcctttag ataagccgtg tcggattcgt
     9121 tt
//
LOCUS       DAWKQB010000079         8921 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.079, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000079 DAWKQB010000000
VERSION     DAWKQB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.079"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            295..993
                     /locus_tag="WJL14_001358"
     CDS             295..993
                     /locus_tag="WJL14_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ7656419.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAH"
     gene            complement(1050..2054)
                     /locus_tag="WJL14_001359"
     CDS             complement(1050..2054)
                     /locus_tag="WJL14_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ7656420.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQILHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASRKGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARATGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2124..4532
                     /locus_tag="WJL14_001360"
     CDS             2124..4532
                     /locus_tag="WJL14_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ7656421.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4637..5671
                     /locus_tag="WJL14_001361"
     CDS             4637..5671
                     /locus_tag="WJL14_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ7656422.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKAAKVAKV
                     AKVAATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQIS
                     ESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNSALKNLSPAQRRDFADN
                     IIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSEN
                     AAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVH
                     ALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRD
                     LHSGAYVDIR"
     gene            complement(6906..8786)
                     /locus_tag="WJL14_001362"
     CDS             complement(6906..8786)
                     /locus_tag="WJL14_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ7656423.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGIKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGTYRPSISIYFDVDNE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDKEDNVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVRHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT         2121 a         2520 c         2416 g         1864 t
ORIGIN      
        1 gaaaggggtg taacccttcc gaaccagggc aacgcatagg gcggtgcttt atgtgccgtc
       61 ctgtgtgttg gaacatcatt agttccccaa cagccgtatc cgtctggggc gacggcgcgt
      121 attgtacgac agaaaccgct tcagacggca tcgggtttga tgccgtccga agcggtttcc
      181 ccaaacgaaa acgtcctttt ccggaggcaa actgtatcaa cacgcggaaa tgcaataaaa
      241 tagccggatt ccgattgatt tccaacatct gtttccaaca tcacggagag ccgtatgaaa
      301 tccaaacacc tcgccctcgg cgttgccgcc ctgttcgcac ttgccgcgtg cgacagcaaa
      361 gtccaaacca gcgtccccgc cgacagcgcg cctgccgctt cggcagccgc cgccccggca
      421 gggctggtcg aagggcaaaa ctatactgtc cttgccaacc cgattcccca acagcaggca
      481 ggcaaagtcg aagtccttga gtttttcggc tatttctgtc cgcactgcgc ccacctcgaa
      541 cctgttttaa gcaaacacgc caagtctttt aaagacgata tgtacctgcg taccgaacac
      601 gtcgtctggc agaaagaaat gctgacgctc gcacgcctcg ccgccgccgt cgatatggct
      661 gccgccgaca gcaaagatgt ggcgaacagc catattttcg atgcgatggt caaccaaaaa
      721 atcaagctgc aagagccgga agtcctcaaa aaatggctgg gcgaacaaac cgcctttgac
      781 ggcaaaaaag tccttgccgc ttacgaatct cccgaaagcc aggcgcgcgc cgacaaaatg
      841 caggagctga ccgaaacctt ccaaatcgac ggtacgccca cggttatcgt cggcggcaaa
      901 tataaagtcg aatttgccga ctgggagtcc ggtatgaaca ccatcgacct tttggcggac
      961 aaagtacgcg aagaacaaaa agccgcgcac taagcccgtt tggaaaatgc cgtctgaaac
     1021 ttggttttca gacggcattt tgattgggtt taaaacgtaa agcccgtttc cagttcttca
     1081 tcgccgacca gttcgaccaa gagcgcgtag agcggggcga gttcggcata acggcgcgat
     1141 acgcggcgca ggtaattgag gaaacgcggg atttcgggac ggtatttgtc tttgccgtcg
     1201 cggtagtaca ggcgtgcgaa gatgcctgca accttcaagt gccgctgcac gcccatccat
     1261 tcgaaccagc ggtaaaactc gtcaaacgcc tcggggacgg gcaagccggt agcccgcgcc
     1321 ttttcccagt agcggataac caagtccaag acaaattctt cttcccattc gataaaggca
     1381 tcgcgcaaca gcgacaccaa atcgtaggaa atcgggccgt aaagcgcgtc ttggaagtct
     1441 aaaacgcccg gtctgccgcg cgtcagcatc aggttgcgga cgataaagtc gcggtgcaca
     1501 tagactttgg gctgcgccaa caggggcggc agcagcgtat cgacggtttg ctgccaaagt
     1561 tggcgttgtt tgaatgttaa ttcgcgcccc aattcttttg cgacaaacca ttccgggaac
     1621 aggttgattt cgcgcaacat cgtttcacgg tcatattcgg gcaaaacccc tttacggctc
     1681 gccttctgca attcgaccaa ctcgccgatg gcctccaaaa gcagggcttt gtgcgccgtt
     1741 tcgccctgtt cctgaagcat cgcggtcaaa aacgtcgtat tgcccaagtc gttcaatacc
     1801 acaaacccca aatccgtgtc cgcgtgcaat atctgcggca cattgaccat gtcaaacagt
     1861 ttctgcactt ttaaataagg cgcgacactc atcttgtcgg gcggcgcgtc catacagacg
     1921 acgctgccgc cgtctgaaaa cgttgcacgg aaatagcggc ggaaatcagc atccgccgcc
     1981 gcgaaggaca gatcgaagtc ccgttcggga tagatggtct gaagccaatt tttcagttcg
     2041 gtttgccgtt gcataacagt actaaagcat ttcaggttac aataaacgct attctaactg
     2101 gcaaaccgac ttgaggggcg attttggctc gtttattttc actcaaacca ctggtgctgg
     2161 cattgggctt ctgtttcggc acgcattgcg ccgccgccga tgccgttgcg gcggaggaaa
     2221 cggacaatcc gaccgccgga ggaagcgttc ggagcgtgtc cgaacccata cagcctacca
     2281 gcctgagcct cggttcgacc tgcctgtttt gcagtaacga aagcggcagc cccgagagaa
     2341 ccgaagccgc cgtccaaggc agcggcgaag catccatccc cgaagactat acgcgcattg
     2401 ttgccgacag gatggaagga cagtcgcagg tgcaggtgcg tgccgaaggc aacgtcgtcg
     2461 tcgaacgcaa tcggacgacc ctcaatgccg attgggcgga ttacgaccag tcgggcgaca
     2521 ccgttaccgc aggcgaccgg ttcgccctcc aacaggacgg tacgctgatt cggggcgaaa
     2581 ccctgaccta caatctcgag cagcagaccg gggaagcgca caacgtccgt atggaaaccg
     2641 aacacggcgg acggcggctg caaagcgtca gccgcaccgc cgaaatgttg ggcgaagggc
     2701 attacaaact gacggaaacc caattcaaca cctgttccgc cggcgatgcc ggctggtatg
     2761 tcaaggccgc ttccgtcgaa gccgatcggg aaaaaggcat aggcgttgcc aaacacgccg
     2821 ccttcgtgtt cggcggcgtt cccattttct acaccccttg ggcggacttc ccgcttgacg
     2881 gcaaccgcaa aagcggcctg ctcgttccct cactgtccgc cggttcggac ggcgtttccc
     2941 tttccgttcc ctattatttc aaccttgccc ccaatctcga tgccacgttc gcgcccggcg
     3001 tgatcggcga acgcggcgcg gtctttgacg ggcaggtacg ctacctgcgg ccggattatg
     3061 ccggccagtc cgacctgacc tggctgccgc acgacaagaa aagcggcagg aataaccgct
     3121 atcaggcgaa atggcagcac cggcacgaca tttccgacac gcttcaggcg ggtgtcgatt
     3181 tcaaccaagt ctccgacagc ggctactacc gcgactttta cggcaacaaa gaaatcgccg
     3241 gcaacgtcaa cctcaaccgc cgtgtatggc tggattatgg cggcagggcg gcgggcggca
     3301 gcctgaatgc cggcctttcg gttctgaaat accagacgct ggcaaaccaa agcggctaca
     3361 aagacaaacc gtatgccctg atgccgcgcc tttccgccga ttggcgcaaa aacaccggca
     3421 gggcgcaaat cggcgtgtcc gcccaattta cccgcttcag ccacgacagc cgccaagacg
     3481 gcagccgcct cgtcgtctat cccgacatca aatgggattt cagcaacagc tggggttacg
     3541 tccgtcccaa actcggactg cacgccacct attacagcct caaccgcttc ggcagccaag
     3601 aagcccgacg cgtcagccgc actctgccca tcgtcaacat cgacagcggc atgaccttcg
     3661 aacgcaatac gcggatgttc ggcggcggag tcctgcaaac cctcgagccg cgcctgttct
     3721 ataactatat tcctgccaaa tcccaaaacg acctgcccaa tttcgattcg tcggaaagca
     3781 gcttcggcta cgggcagctt tttcgtgaaa acctctatta cggcaacgac aggattaaca
     3841 ccgcaaacag cctttccgcc gccgtgcaaa gccgtatttt ggacggcgcg acgggggaag
     3901 agcgtttccg cgccggcatc gggcagaaat tctacttcaa aaacgacgca gtcatgcttg
     3961 acggcagtgt cggcaaaaaa ccgcgcagcc gttccgactg ggtggcattc gcctccagcg
     4021 gcatcggcag ccgcttcatc ctcgacagca gcatccacta caaccaaaac gacaaacgcg
     4081 ccgagaacta cgccgtcggt gcaagctacc gtcccgcaca gggcaaagtg ctgaacgccc
     4141 gctacaaata cgggcgcaac gaaaaaatct acctgaagtc cgacggttcc tatttttacg
     4201 acaaactcag ccagctcgac ctgtccgcac aatggccgct gacgcgcaac ctgtcggccg
     4261 tcgtccgtta caactacggt tttgaagcca aaaaaccgat agaggtgctg gcgggtgcgg
     4321 aatacaaaag cagttgcggc tgctggggcg cgggcgtgta cgcccaacgc tacgttaccg
     4381 gcgaaaacac ctacaaaaac gctgtctttt tctcacttca gttgaaagac ctcagcagtg
     4441 tcggcagaaa ccccgcagac aggatggatg tcgccgttcc cggctatatc cccgcccact
     4501 ctctttccgc cggacgcaac aaacggccct gaccgtcgga aacccggcag gagcaccgtt
     4561 cccgcacaag acggcattcc accgacaacc ccaaacccgc catcaaaggc aggattcaaa
     4621 cgataaggaa agaatgatga aaatcaaagc cctgatgatt gccgccgcat tgctggcagc
     4681 agccgatgtc cacgccgcac cgcaaaaggc aaaaaccgca tccgccaaag ctgccaaagc
     4741 tgccaaagct gccaaagttg ccaaagttgc caaagttgcc gccacggcgc aaaaagaagc
     4801 cgcacccgca caacagcagg gcggtatccg cttttcagac ggcattgccg ccgttgccga
     4861 caacgaagtc atcacgcgcc gccggcttgc cgaagccgtt gccgaagcca aagccaacct
     4921 gcccaaagac gcgcagataa gcgaatccga gctgtcccga caggtgctga tgcagcttgt
     4981 caaccaatcc ctgattgtac aggcgggcaa acgccgcaac attcaagcaa gcgaagcgga
     5041 aatcgatgcc gtcgtcgcaa aaaattccgc cctcaaaaac ctcagccccg cccaacgccg
     5101 cgattttgcc gacaacatca ttgccgaaaa agtccgccag caggcagtga tgcagaacag
     5161 ccgcgtgagc gaagctgaaa tcgatgcctt cctcgagcag gcgcaaaaac aaggcatcac
     5221 cctgcccgaa ggcgcaccgt tgcgccaata ccgcgcccaa cacatcctga ttaaagccga
     5281 cagcgaaaac gccgccgtcg gcgcggaaag caccatccgc aaaatctacg gagaggcccg
     5341 cagcggcaca gacttttcca gcctagcgcg ccaatattcg caagacgcga gcgcgggcaa
     5401 cggcggagat ttgggctggt ttgccgacgg cgtgatggtt cccgcctttg aagaagccgt
     5461 ccacgcgctc aaacccggac aggtcggcgc gcccgtccgc acccaattcg gctggcatat
     5521 catcaaattg aacgaagtgc gcgatgccgg cacacctcag gaacgtatcc gcaattccgt
     5581 gcggcaatac atcttccaac aaaaagccga acaggcaacc gtcaacctgt tgcgtgacct
     5641 gcattccggc gcgtatgtcg acatccgcta aggcggtttg aagcaaaaag ccataccgat
     5701 cggcaaaaat ccgggcggta tggctttttg gatttcgagt tacttttaca ccgtcattca
     5761 tcattcccgc gaaagcggga atctagaaac gaaaagtaac aggaatttat cgggaatggc
     5821 tggagtttaa aggactggat tcccgccgtc gcgggaatga cgggattttg ggttgtggta
     5881 atttatcgga aaaacaaaaa aacctatgcc gtcattcccg agcaggcggg aatccggtta
     5941 tttaaaactg cagaaattta tccgaagcaa caacaatctt tccatcgtca ttcccgcgta
     6001 ggcgggaatc tagcaacgaa aagtaacagg aatttatcgg gaaaacagaa acctctatgc
     6061 cgtcattccc gcgaaagcgg gaatctagga cgtagaatct aaagcaaccg ttttatccga
     6121 taagtttctg tgccgaaggg tctggattcc cgctttcgcg ggaatgacgg cgcataagtt
     6181 cccgtgcgga cagacctaga ttcccacctg tgtgggaatg acgattcaga agttgcccga
     6241 aactcaaaaa aaaacgaaac cgaacgaacc ggattcccgc tttcgcggga atgacggcgc
     6301 ataagttccc gtgcggacag acctagattc ccacctgtgt gggaatgacg atgcagaagt
     6361 tgcccgaaac caaaaaactg aagccgaacg gtctggattc ccgctttcgc gggaatgacg
     6421 gcgcataagt tcccgtgcgg acagacctag attcccacct gcgtgggaat gacgattcag
     6481 aagttgcccg aaactcaaaa aaaacgaaac cgaacgaacc ggattcccgc ttttacggga
     6541 atgacgggat tttgggttgt ggtaatttat cgggaaaacg gaaaccccta tgccgtcatt
     6601 cccgcgcagg cgggaatcta ggacgtagaa tctaaagaaa ccgttttatc cgataagttt
     6661 ctgtaccgaa gaatctggat ttccgctttc gcgggaatga cggcatatca gtttctgtgc
     6721 ggataaacct agattcccat ctgcgtggga atgacgggat tttagattgc gggtatttat
     6781 cgggaacggc ggcttggaag ttcattgaaa cggaaaaaca acggaaaccc aaaaaaccgg
     6841 attcccgact gtgggaatga tgagattcag gtttctgttt ttgccggagt ttgccgtatc
     6901 gggcttcaga cggcattgcc tgccgttgta cccgcgggtg cgactgcctt gatgtagttg
     6961 agcgagacaa actgcttctc ggcatctaat tcggtgattt tgaacaatgc ctgcgatttg
     7021 ggcagcgcgt caaacggaat accggtcgcg cgtgttacta gcggcagacc ttcgatgcgg
     7081 acgaggtctt ctttcagaat cgtcgccgtc agctcgctcg tgccttgctg ttgcaggtaa
     7141 accaggctcc agtaggcttc catttggcgt tggaagtcgg cgtaggcggt ataggcggca
     7201 tcaaagtcgc gcagtgcggc gaaaagctcg gcatcgctgt tttgatacag cggctcggca
     7261 gtgtcgtcta tcaggctgat cagctgcttt tggttgatgt agtcggcggc gcggcgcagc
     7321 ggcgaggtaa accagccgta atgccgcacg cccatgccga tatgcggctc ggatttggtg
     7381 ctcatgcgta cttttccggt gggttggacg cggaagaggc cgggcaggtc gttgtcatgg
     7441 agcatttgcg cccaagtgct gttggcaaga atcatcatct cgctgaccag cgtatcgatg
     7501 ggcgaaccgc gttcgcggcg gacgacggat acgttgtctt ctttatccaa ttcgatgctg
     7561 taatcgtatt gcggcgcgcg gtcgggttcg tatttgccgc gcgctttttg cagggcggtg
     7621 gcgaattgat aaaaccaaat caggtcttga tggtgggcga acatcatttc gcccgcttcg
     7681 tccaagcccg cttcggcgtt gaaatgcggt tcgatggctt ggatacgcag gttttcagcg
     7741 atgttgaccg cttcgatttt gcaggtcggc gcgccgacgt tgaactcgtt gtccacatcg
     7801 aaataaatgc tgatggaagg gcggtatgta cctgcatcaa ggctgaacgc ggcaatccag
     7861 ttttcgggca acatcgtgat tttgccgccg ggaaaataaa ccgtgcttaa gcgttccatg
     7921 atgttttttt ccattttgtc gccttgctta atggcaagcg acggcgcggc aatgtggatg
     7981 ccgacgcgtt ttatgccatt gcccaaatcg gtcaggctta aagcgtcgtc cacttcggta
     8041 gtcgattcgt cgtcaatgga aaaggcggta acgtcggctt tgggtaagtc aggcatttca
     8101 ggaagcggaa ggtcggggaa gcctgttcct ttggggaaat atttgatttc aaacccgtct
     8161 tgcaggtatt ggggaatgga cgtaatgccg cccgtttttt tcgccaattc gtaggcagag
     8221 gttttcagcg cgtcggcggc tttggtaaag gctttgtagg tcagcgactg cttgtcgggc
     8281 gcgtgcagga tggttttcaa atccgccgcg atttcagacg gcatctcgcc gcgtttcaag
     8341 gcttccgccc aagcgtcgat ttgcgcgtct tgctgttttt tgcgttcgat ggcggcaagt
     8401 gcttgtttta aagtttcttc gggcgcggct ttgaacacgc ctttggcttt tttgtagaaa
     8461 tacatcggcg cggcgtaaag cgcaatcaaa gttgccgcca gctcggtttt ggtcggcgca
     8521 tggccgtaat attcttcggc gatggcttcg gcggtaaatt cctcttcgcc gcatacttcc
     8581 cacaataaat cggtgtcgat gtccgccgcc tgtgcctgcg cgttttccaa aaacgccgcc
     8641 atatcgccgt caaactcggc aaagacgttg ttcgccttca ctttggtgcg tttgccgtgt
     8701 ggggtatcga cttggtaggt ggcatcgttt ttttggatga tggcggcgat tttgaattgg
     8761 ccggactctt cgtaaaaaat attcattttt cggatttttc tgtggaaact caagcgggcg
     8821 attttagcag attaccgaaa atgccgtctg aaaaaaggtt gggagagggt tggcgcgtct
     8881 ttgcggtgct tgcgttatag tggattaaca aaaaccagta c
//
LOCUS       DAWKQB010000080         8913 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.080, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000080 DAWKQB010000000
VERSION     DAWKQB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8913
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.080"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..230)
                     /locus_tag="WJL14_001363"
     CDS             complement(<1..230)
                     /locus_tag="WJL14_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7656424.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAVLFY
                     RKIRTVINHHLALAADEVFEGSVELDESYFGGR"
     gene            complement(916..1881)
                     /locus_tag="WJL14_001364"
     CDS             complement(916..1881)
                     /locus_tag="WJL14_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ7656425.1"
                     /translation="MRNAVGLDISAKTFDVVTIINGETDYRKFSNDEQGCKNLKEWIS
                     AKREKDIYVCMEATGNYYEQAADCLAEEYHVSVINPLKIKAYAQKRFSRVKNDKQDAK
                     LIAEFCQTALIEELPKREKPTEQQYSLKRLLSLQSQLLEQQTSQKNRIKAAKDSFVQK
                     IHEKQLKELENHLNAVKKKIDQTIKSDKKMKELTKRLETIPSVGKTTAISLMSYLINS
                     TFENAKQFTAYAGLNPHQNISGTSVNKKSKMTKYGNRRIRGSLFMAALVAFKNNYFPA
                     FTNRLKKAKKPKMLIIGALMRKILVVAFNLYKTETDFDKTRYQTA"
     gene            complement(2742..3800)
                     /locus_tag="WJL14_001365"
     CDS             complement(2742..3800)
                     /locus_tag="WJL14_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ7656426.1"
                     /translation="MIYLITGNMGTGKTSKAVSMILNNEDGLFKMQLEDGTEVDRPLY
                     FCHIDGLDAKKFNAHELTEEQIMSAPLRDIIPDGAVLIVDEAHYTYPVRSSGRAVPPY
                     IQELTELRHHGHTVIMMTQHPQQLDIFVRNLISKHIHLERKALGTKQYSWYKCVTSLE
                     NPAAVSNTESKNFTPPKEAFKYYKSSSQHIKFKKKVPLAVWALILIIGFIGWKIYTVY
                     GISQKAINPEQFETTSQVSAASETINQEADLNTAAQSQKINQDLKADDFIPTLAEKPE
                     SKPIYNNVRQVKTFERIAACIEGGKTGCTCYSDQATPLEEVTKEMCKQYARNGLPFNP
                     YKEQDNSANNAQKAPTTS"
     gene            complement(3803..4090)
                     /locus_tag="WJL14_001366"
     CDS             complement(3803..4090)
                     /locus_tag="WJL14_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ7656427.1"
                     /translation="MPAALIPLIAFLLRMLIVRIIIATGLTFVSFAGYLVALNKFKEY
                     FLNAANSMPGDIYNLLLIGGFGTGFNYLFGAFSFWVSMKALNKLTTVLPRG"
     gene            complement(4092..5570)
                     /locus_tag="WJL14_001367"
     CDS             complement(4092..5570)
                     /locus_tag="WJL14_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ7656428.1"
                     /translation="MLGMFSVNSYAERFKYPIGNSDVRLDIDHTKSVVTDFRVDGQRF
                     SGRIIEPSIIEHVPTGARSLEKIPVKVTASVSRAGVLAGVGALVRQGAKLGKRAVPYV
                     GTALLAYDIYETFKDEIKEQGYQYDPETDKFVKGYEYSNCIWEHAENGIKTYGCYGVD
                     SSIMRLMSDYSRFPEVKELMEHQMEIVGRNYWEMVRKNRNDSFRNYNFSRCYFNWNGG
                     NCNIGEDINDARSFINFSLIRNPKYKEEMDAKKLEEILALKVDANPDKYIQATGYPGY
                     SEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEA
                     PETKPKPAPTPETNPKEKENPREEDQDNPKPTPTPGETPSPNESPKDRREEKKPDGNG
                     GLLCDLFPKILACAEMGEPSENDFEGIAIPKAVNEETWSPDNMFPSSGVCPKDKTFHV
                     FGKAFSVSYQPLCTLMENVRFAVIIGFIIMSAFITFGSLRKE"
     gene            complement(5548..5853)
                     /locus_tag="WJL14_001368"
     CDS             complement(5548..5853)
                     /locus_tag="WJL14_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7656429.1"
                     /translation="MFITEAQLALYKYQPSSKYFGKTMSSTFASELLEYSKVNKFIIH
                     EAIQWFLNIRISNNIWKIYFSDESVAYIKILELQDDSSRGIEIKTFDFNINAGDVFG"
     gene            complement(5982..6257)
                     /locus_tag="WJL14_001369"
     CDS             complement(5982..6257)
                     /locus_tag="WJL14_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656430.1"
                     /translation="MYYQVGNQCLEQNQAENVYFSLVVPKITEDGKIIKPEYNGKDWK
                     IDGQIIKADLPKCDPSENLKDGMYIGWLIFGIVASVYFVTVIKRLLR"
     gene            complement(6268..6486)
                     /locus_tag="WJL14_001370"
     CDS             complement(6268..6486)
                     /locus_tag="WJL14_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656431.1"
                     /translation="MKIMNNLKKYGAKIAVFAAAPLALAANTYAALPETAKQALEGAK
                     ADGLEAGWIVIGVFAALFVIAIVKRVMR"
     gene            complement(6569..6772)
                     /locus_tag="WJL14_001371"
     CDS             complement(6569..6772)
                     /locus_tag="WJL14_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656432.1"
                     /translation="MSRYQQKFIVQELENYEFIFPDQFGDIGFTQNLKEAGQYENYED
                     AFNAGLEEIGGHFQIFSFYIREE"
     gene            complement(6790..7101)
                     /locus_tag="WJL14_001372"
     CDS             complement(6790..7101)
                     /locus_tag="WJL14_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656433.1"
                     /translation="MFDQLQVTTYPATLLGAKQFKGEIDGNKIDTCTVLVASPMPANG
                     NSVGFTAASMKFGESHNFDRLKNLKFPCSVNVTVAMESTGKGLVPKLLDFEVKGASTK
                     A"
     gene            complement(7122..8471)
                     /locus_tag="WJL14_001373"
     CDS             complement(7122..8471)
                     /locus_tag="WJL14_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ7656434.1"
                     /translation="MRAEDRAETAGACVGAPCAPQTATPLNAPPRLIGGEQKNPNLQG
                     GEQPIEILESHQVEFEYFSQYVTDGKGRLIEIPLRRGREDGAFIDQITFTIHEESIPK
                     VTQKAYVTDAEYLAKYSELLQEILGFGISAKLPYKGKFFYQSCYQLGPTNVEYGKVHY
                     GGQRETILVELNGTGCTAAKPGWENRLYEFLQKCVRPKITRVDVAHDFFNGEYTPQHA
                     LTDHDNGFFDCHNVRPKSECKGVAWRHEDGSGKSFYIGKRGNAKYTRIYEKGKQLGDK
                     SSPWVRFETEFRNGDIEVPIDILIHAGSYIGGAYPVCEQIFKCEAKRMDSTVKEVNLT
                     FEHKQFYARQQVGKWVNFLKDIGWTAEDIVKDLTKGVEGYPKGLQPEEYDSSNACAFY
                     IHEEQKAINALNIEVLELELNQEKQYEYPQDRQKQHERDYDKEQYQISDQLEDWKYL"
     gene            complement(8468..8683)
                     /locus_tag="WJL14_001374"
     CDS             complement(8468..8683)
                     /locus_tag="WJL14_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656435.1"
                     /translation="MCIAFYTVNPDQYEYEKSRAYIVRIFVNDEIVETTVFPITNPQN
                     TYKTRQEAEEYGRLTVKALMDKQEKTA"
BASE COUNT         2099 a         1831 c         1920 g         3063 t
ORIGIN      
        1 cgtccgccga aatagctttc gtccaactcg acagagccct caaaaacctc atcggcagcc
       61 aaggctaaat gatggttgat aaccgtgcgg attttacggt agaacagtac tgccgaattg
      121 ggatggatac ccaaaatatc ggcagcggaa cgggcggtaa cttcgagtac aaaaaaacgg
      181 agcagttctt tttgtacttt tttctttaat ttgcagtgcg ttatcttcat atttcgaggg
      241 taacatatct gctaatctag tacagcccca aaaatatacc aaaaacagca aaacaaattg
      301 taaggatacg tataggcttt gtaaaggtaa attgtgaaaa aagcagtttt ttaaacgaat
      361 gaaacggctt cgggctgaaa tatatgctga tgccctgtcc ttcccgtatt tctcgtgtgt
      421 tgtcaaagtg caggctgctt tgaaatcggt attgccatct atgaaccacc actttgcttt
      481 atttcagcgg gtttgagatg tatataagaa tattgttttg aataagttta aaaaaattga
      541 taaccattct tgacggtttt ttaaggaaag agtagagtgc caattctatc ttgtgtgata
      601 cacgaagcaa caaggaaaac tatctagaat gtccttgtat gggcatagat catagatcat
      661 ttccaggctg cctgtggttg ttttaacttt aagatggagg ggtttgccta gaacatcagt
      721 gcttatctcc aagacagcct tcccaatccc ttgtgcctct taataaagat gtttcttgat
      781 tattcaaaca aaataaattg taaatttatt atcttgttgt tttcattggt tatttagttt
      841 tgcatttttt cggaatacaa aaatccaagc ggatttttta caatccgctt ttgctatttt
      901 tatcagttcg gttagctaag ccgtctgata acgtgtttta tcgaagtcgg tttctgtttt
      961 ataaagatta aatgcaacga ctaaaatttt tctcatcaat gcgccgatga tcagcatttt
     1021 gggctttttg gcttttttca gcctgtttgt gaatgctgga aagtagttgt ttttgaatgc
     1081 gactaatgcg gccatgaaca gggatccgcg tattcttcgg tttccgtatt tggtcatttt
     1141 gctttttttg ttgacgcttg tccctgatat gttttgatgc ggatttaatc cggcataggc
     1201 tgtaaactgc tttgcgtttt cgaatgtgct gttaataagg tagctcatta gcgatatggc
     1261 tgttgttttg cctactgacg gtattgtctc taaccgtttt gtcagctctt tcattttttt
     1321 gtctgatttg attgtttggt cgattttctt ttttactgca tttagatggt tttcaagttc
     1381 ttttagctgt ttttcatgta ttttctgtac aaatgaatct tttgctgctt tgattctatt
     1441 tttttgcgat gtctgttgtt ctaaaagttg gctttgaagt gatagcagtc ttttcaagct
     1501 gtattgctgt tcagtcggtt tttctctctt tggcagctct tctattaatg ctgtttggca
     1561 aaactctgcg attaattttg cgtcttgttt gtcgtttttg actctgctga atcgcttttg
     1621 tgcataggct ttgattttta gcgggtttat tactgatacg tggtattctt cggccaagca
     1681 gtctgcggcc tgttcgtagt agtttcctgt tgcttccatg catacgtata tgtctttttc
     1741 tctttttgcc gatatccatt ctttcaggtt tttgcagcct tgttcatcgt tgctgaattt
     1801 tctgtaatcg gtttcgccgt tgattatggt aacgacatca aatgtttttg cggatatgtc
     1861 taatcctact gcgtttctca tgttaatttt ccttacgatt caggctttaa tgcctttgat
     1921 gtttctcaat ttcaaacata agaaaaccgc cgtccttatc tttctttcaa tcttgcgatt
     1981 aggaagtaat caggttcggc ggctttgtcg tcggttagct attccgagac gtttgaagta
     2041 tatcagaatc gtatgaatta attttttgta tttgctggtt tatttaaaaa tttctgttgc
     2101 gacccgctgc attgtgtttt tttactgctt tcagtccggt tttcgccggt atggggtttg
     2161 tgtgcgattg gagaacaacc gataaaaagg tgcaaggggg tattcataaa gatttggaaa
     2221 aaaacactcc aaatctttac aaaacttccc ccttgcacct ttttatcggc cgttctctgt
     2281 caaacgcacc cccatacctc agcgaaaacc ggactgaaag cagtaaaaaa aaacaattcc
     2341 gctagatatt ttcccccgcc ctagctcctc cgtcctgaag ggctgggaaa ggtttctgaa
     2401 actcaaacag gcgcggcaag cgcggaaagg tagattgata tgacttagat ttgatgatat
     2461 gacaatttgt agagcggtgg ggataagcaa aatccccgag cgcgaactag gggatttttg
     2521 cattcctgcc aagttttaaa ctttacagga aattttaaaa tgcgaattac cgttagcctc
     2581 ggaaaactgc atttgactgt atcggtagat acgcggttta ttctagccgt gttcatgctg
     2641 tttagtcaat aacccacgat tagaaggccg ccatgcagcg gcctttctta attcgctgcg
     2701 ccgacaaccg cgccgctttg cggctcgaac ttcgcgcccg cttaggacgt tgtaggggct
     2761 ttttgcgcgt tgtttgcgct gttgtcctgt tctttgtatg gattaaaggg taggccgttt
     2821 cttgcgtact gtttgcacat ttcttttgtt acttcttcca gcggtgtggc ttgatcgctg
     2881 tagcaggtgc agccggtttt tccgccttcg atacaggccg ctatgcgctc aaaggttttg
     2941 acttggcgga cgttgttgta aatcggtttg gattcgggtt tttcggccaa cgtcgggata
     3001 aaatcgtctg ctttcaaatc ctgattgatt ttttgtgatt gtgctgctgt atttaaatct
     3061 gcttcttgat ttatcgtctc tgatgctgct gaaacttgtg atgttgtttc aaactgttct
     3121 ggatttatgg ctttttgtga tattccataa actgtataga ttttccagcc gataaatccg
     3181 attatcagaa ttaaagccca aacggccaac ggtacttttt tcttaaattt gatgtgctgg
     3241 cttgatgatt tgtagtattt aaatgcttct ttgggcggtg tgaagttctt cgattctgta
     3301 tttgaaacgg ctgcgggatt ttctaagctg gttacgcatt tgtaccacga atattgtttt
     3361 gttcctaatg cttttctttc taaatggatg tgcttggata tcaggtttct aacgaagata
     3421 tcaagctgct gcgggtgttg tgtcatcatt ataaccgtat ggccgtgatg tcttaattct
     3481 gttaattctt gaatataagg tggtacggct cggcctgatg atcttaccgg ataggtatag
     3541 tgtgcttcgt ctactatcag aaccgcgcca tcagggatga tgtctcttag cggcgcggac
     3601 atgatttgtt cttccgttag ttcgtgtgcg ttgaactttt ttgcgtctag tccgtcgata
     3661 tggcaaaaat ataagggccg atctacttct gttccgtctt cgagttgcat tttaaacaat
     3721 ccgtcttcgt tgtttaaaat cattgaaact gcttttgatg tttttccggt tcccatgttg
     3781 ccggtaatca gataaatcat ttttatcccc tcggtaatac cgttgtcagt ttgttcaatg
     3841 ctttcatgct tacccaaaaa gagaacgcgc cgaaaagata gttaaaacct gttccgaatc
     3901 cgcctatcag tagaaggtta taaatgtctc caggcatgga gtttgccgca tttaaaaagt
     3961 attctttgaa tttatttaac gcaaccaaat accccgcaaa cgatacaaat gtcagacctg
     4021 ttgcaataat gatccgaacg atcagcattc ttagcagaaa tgcaattaat ggaattagag
     4081 cagccggcat tttattcctt tctcaatgat ccgaatgtga taaatgcgga catgatgata
     4141 aagccgataa ttacggcgaa tcttacgttt tccattaatg tgcataatgg ctgataactt
     4201 acgctaaatg cttttccgaa aacgtggaat gttttgtctt tcggacaaac tccgcttgac
     4261 ggaaacatgt tatcgggcga ccacgtttct tcatttacgg ctttgggtat ggctatgcct
     4321 tcaaaatcgt tttctgaagg ttcgcccatt tcggcacagg ctaggatttt aggaaagaga
     4381 tcacagagca atccgccgtt tccgtctggt ttcttttctt ctcgccgatc ttttgggctt
     4441 tcgttcgggc ttggtgtttc gcccggtgtg ggcgtgggtt tgggattgtc ttgatcttct
     4501 tcgcgcgggt tttctttttc ctttggattg gtttcgggcg taggcgctgg ctttggcttg
     4561 gtttcaggtg cttctgcgct tccgggtgtg aggtcgggac gcgggattac ttgaacatcc
     4621 actgtggtgt tgccttgcga atccctgccg aatgttgcga caacctgaac gggattcccg
     4681 ttcctgtccg tgacgggccc catattcact tttgttccgg gtgcgacttc tactttttcg
     4741 gaataaccgg gataaccggt tgcctgtatg tatttgtcgg gattggcatc gacttttaag
     4801 gctaaaatct cttccagctt tttggcatcc atttcttctt tgtatttcgg attgcgtata
     4861 agggaaaaat tgatgaaaga tcttgcgtca tttatatctt caccgatatt gcaattaccg
     4921 ccgttccagt tgaaataaca acgactaaaa ttgtagttcc taaaagaatc attcctattc
     4981 ttgcgtacca tttcccaata attgcgcccg acaatttcca tttgatgctc catcagttct
     5041 tttacttccg gaaatctgct gtaatctgac ataagacgca taattgaact atcaacgccg
     5101 taacagccat aggttttaat accgttttcg gcgtgttccc aaatgcaatt actgtattcg
     5161 tagcctttta caaatttatc ggtttcggga tcgtattgat agccttgttc tttaatttcg
     5221 tctttaaatg tttcgtagat gtcataggcc aagagcgcgg ttccgacata tggaaccgcc
     5281 cttttaccca gttttgcgcc ttgacggact aatgcgccaa cgcctgccaa gactccggcg
     5341 cgggatactg atgcagttac tttgacagga attttttcaa gagagcgtgc acctgttggc
     5401 acgtgttcta ttattgaagg ttcgataatt cgacctgaaa aacgctgacc atcaacacga
     5461 aaatctgtta ctacagattt tgtatgatca atatccaatc taacatctga atttccaatg
     5521 ggatatttaa aacgttcggc ataagaatta accgaaaaca tccccagcat taatattaaa
     5581 atcaaacgtt ttaatttcaa ttccacgact agaatcatcc tgtaattcta aaatttttat
     5641 atacgcaaca gactcatcag aaaaataaat tttccaaata ttattagaaa tccttatatt
     5701 taaaaaccat tggattgctt catgaattat aaatttattt acttttgaat attctaaaag
     5761 ctcactagcg aaagtagatg acattgtttt gccaaaatac ttgctggacg gctggtattt
     5821 ataaagtgcc aactgggctt cagtgataaa catgattcca acttcctttt ttgttgttcg
     5881 gaagtcggat tatctgccta accgttttga atatcaatcc ttgaacagga tggcggcaat
     5941 gatgatcgga acggaaagac cgacatagaa atagaaatcc atcatctcaa gagcctttta
     6001 atcacggtta cgaaatagac ggacgccacg attccgaaaa tcagccagcc tatatacatg
     6061 ccgtctttca ggttttcgga cgggtcgcat ttaggtaaat cggccttgat gatttggccg
     6121 tctattttcc aatctttgcc gttgtattcg ggttttatga ttttcccgtc ttcagttatt
     6181 ttaggtacta ccaagctgaa atagacgttt tcggcctgat tctgctctaa acactgattt
     6241 ccaacttggt agtacatttt gccgacctta tcgcattaca cgtttcacaa tcgcgatcac
     6301 aaacagtgcc gcgaatacgc cgataacgat ccaaccggct tctagaccgt cagcttttgc
     6361 gccttctaat gcttgttttg cagtttcggg caatgctgca taggtattgg ctgccagtgc
     6421 taacggagca gctgcgaaaa cggcgatttt cgcgccgtat tttttcaggt tattcatgat
     6481 tttcatgatg ttttccttag ttggttaaaa aaatggtttc gcgggctttg tgaaggtttt
     6541 acagaccgcc cgccgagcct gttaactttt attcttctct gatgtagaag ctgaaaatct
     6601 gaaaatgtcc gccaatctct tctaatccgg cattgaatgc gtcttcataa ttttcatact
     6661 gtccggcttc tttcagattt tgggtaaatc caatatcgcc gaactgatcg gggaagatga
     6721 attcatagtt ttccagttcc tgaacgataa atttttgctg atacctgctc atattctgga
     6781 ctttctgcct taggctttgg tcgacgcgcc tttcacttca aaatcaagca gcttgggtac
     6841 taagcctttg ccggtagatt ccattgcgac ggttacattt acggagcagg ggaatttgag
     6901 gtttttcagc cgatcgaaat tgtggctttc tccaaatttc atgctggcag cagtaaaacc
     6961 tacgctgttg ccgttggcgg gcatggggct ggctaccagt acggtacagg tatcgatttt
     7021 gttgccgtca atctcacctt tgaactgttt tgcgcccagg agcgtggcgg gataggtggt
     7081 tacttgtaat tgatcaaaca tgggtatttc ctttctttga tttatagata tttccagtct
     7141 tctaactggt ctgatatttg atactgctct ttgtcgtaat cgcgctcatg ctgtttctgc
     7201 ctatcttgcg gatactcgta ttgcttttcc tgatttaatt caagctctaa gacttcgata
     7261 ttcagggcgt ttatggcttt ttgctcttca tgaatataga aagcacaggc gttgctactg
     7321 tcgtactctt cgggctgaag acctttggga tagccttcaa cgcctttggt taggtctttg
     7381 acgatatctt cggccgtcca accgatgtct tttaaaaaat tgacccattt gccgacttgt
     7441 tgccttgcgt agaattgttt gtgttcaaac gtgagattga cctctttaac tgttgaatcc
     7501 atgcgcttgg cttcgcattt gaatatctgt tcacagacgg gatacgctcc gccaatatac
     7561 gaaccggcat gaatcaatat gtctatgggg acttctatat ctccgtttct gaactctgtt
     7621 tcaaatcgaa cccacgggct tgatttatcg cctaactgct tgcctttttc gtaaatccgg
     7681 gtgtatttgg cgttgccgcg tttgccgata tagaagcttt tgccgcttcc gtcttcgtgt
     7741 ctccaagcaa ctcctttgca ttcgcttttc gggcggacgt tatggcagtc aaaaaagccg
     7801 ttatcgtggt cggttaaagc gtgttggggc gtgtactctc cattgaaaaa atcatgtgct
     7861 acgtcaaccc ttgttatttt tggccggaca cacttttgca gaaattcata aagcctgttt
     7921 tcccaaccgg gttttgctgc tgtgcagccg gtgccgttca gctctacgag tatggtttct
     7981 ctttggccgc cgtagtggac tttgccgtac tcgacatttg tagggcctag ctgatagcag
     8041 gactgataga aaaatttgcc tttgtatggt agtttggcac tgatgccaaa acctagaatt
     8101 tcctgcagca attcgctgta ctttgcgagg tactcggcat ctgttacata ggctttttgg
     8161 gttactttgg gtatggattc ctcatgtatc gtaaatgtga tctggtcgat gaatgcaccg
     8221 tcttccctgc cgcgtcttag cggaatttct atcagacggc ctttaccgtc ggttacgtat
     8281 tgagaaaaat attcaaattc gacttgatgc gattcaagaa tttctatggg ctgttcccca
     8341 ccctgcagat tagggttttt ttgctccccc cctattagcc tagggggggc attcaacggc
     8401 gtggccgttt gcggcgcgca aggcgcgccc acacacgccc ccgccgtttc tgctctgtct
     8461 tcggctctca tgcggttttc tcctgcttat ccataagagc cttgacagtc agacggccgt
     8521 attcttcagc ttcttgcctt gttttatatg tgttttgcgg gtttgtaata ggaaatactg
     8581 ttgtctcgac aatttcgtca tttacgaaaa tgcgcacaat gtaggcgcgt gatttttcgt
     8641 attcgtattg atcgggatta acggtataaa aagcgatgca cataagaaag ccccttcact
     8701 ggctagagtt aaaggggctt gatgtttgta aagacttgcg ccccttgatg gggtgcaata
     8761 tataagaagt gattaggaag taattagtgt gtgaaactat agttcctttt atagctttcc
     8821 aataaaatta aagtatttaa aagcttgttt agttccaatt tgttaatttc tgtatctaaa
     8881 taatgttcag aatagtatta ttttttaaat aaa
//
LOCUS       DAWKQB010000081         8855 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.081, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000081 DAWKQB010000000
VERSION     DAWKQB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.081"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(61..1203)
                     /locus_tag="WJL14_001375"
     CDS             complement(61..1203)
                     /locus_tag="WJL14_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ7656436.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKYYAG
                     WLAAMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1252..1674)
                     /locus_tag="WJL14_001376"
     CDS             complement(1252..1674)
                     /locus_tag="WJL14_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656437.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1667..2476)
                     /locus_tag="WJL14_001377"
     CDS             complement(1667..2476)
                     /locus_tag="WJL14_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ7656438.1"
                     /translation="MTQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPDQWMGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLYGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2553..2930)
                     /locus_tag="WJL14_001378"
     CDS             complement(2553..2930)
                     /locus_tag="WJL14_001378"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ7656439.1"
                     /translation="MIYGIGTDIVSLKRIVRLSKKFGQAFAERILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYAPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFAVAEK"
     gene            complement(3131..3898)
                     /locus_tag="WJL14_001379"
     CDS             complement(3131..3898)
                     /locus_tag="WJL14_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7656440.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGYSGLIKL"
     gene            complement(3908..4687)
                     /locus_tag="WJL14_001380"
     CDS             complement(3908..4687)
                     /locus_tag="WJL14_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ7656441.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQSIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFLDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRRDCIYKGIEENRNKVSKEMVLALDYLVNVIKNAS
                     DIQEFSAQDDDLIIIDNVNGLHAGTDYTDKNRHYIRARITV"
     gene            complement(4734..5462)
                     /gene="pdxJ"
                     /locus_tag="WJL14_001381"
     CDS             complement(4734..5462)
                     /gene="pdxJ"
                     /locus_tag="WJL14_001381"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ7656442.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5486..6277)
                     /gene="recO"
                     /locus_tag="WJL14_001382"
     CDS             complement(5486..6277)
                     /gene="recO"
                     /locus_tag="WJL14_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ7656443.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVLVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6311..7438)
                     /gene="pheA"
                     /locus_tag="WJL14_001383"
     CDS             complement(6311..7438)
                     /gene="pheA"
                     /locus_tag="WJL14_001383"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ7656444.1"
                     /translation="MLECTANHRSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7477..8709)
                     /locus_tag="WJL14_001384"
     CDS             complement(7477..8709)
                     /locus_tag="WJL14_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ7656445.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYHVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
BASE COUNT         2090 a         2386 c         2302 g         2077 t
ORIGIN      
        1 ggccggatat taaaaaaagc cgtctgaaca tttttcagac ggcatttttg tacactttcc
       61 tcaacggttc gatccctgca ccatatcaat caggaacagg cggcaatcga ggttgccgtt
      121 ataaagcgga attttgcgtt ttggcgacag gcgcatgaat ttgggcattt cccgatctcc
      181 ggtaaacatt gccgccaacc agcctgcgta atatttcttc aaccacgtcc ccaactgcgg
      241 ataaagcgcc tgcaaggcgc ggacttcctc aaggcgcacg ccgtagggcg gattggacac
      301 cataatgccg cccacgccgt tcggacggac ggattgcgcg tcggcaacgc tgaaggaaac
      361 gatgtcgtcc accccggcac tgcgtgcgtt ggccaatgcc gtctgaatga tgcggcggtc
      421 gctgtcgctg cccgaaatcg gtgcaaaagc cggtttaacc tgcgcttcgg cacggcggcg
      481 caaatccgac cacagcgttt tatcgaaatt ttgcagtttt tcaaaaccga aacggcgcat
      541 catacccggc gcgcggcggg cggcaatcca agcggcttcg atagcaatcg tgccgctgcc
      601 gcaaaacggg tcttgaaacg gctgcgtgcc gtcgtagcct gccgagagca gcaatccggc
      661 ggcaaggttt tcgcgcagcg gggcttcgcc ggtatcgaga cggtagccgc gtttgaacag
      721 ggcttcgccc gaagtgtcga tgaagatttc gacattgcgt tcgtccaaaa aggcgtggat
      781 gcgaacatcg ggcgcggctt tgtccacgct cggacgtgcg tcgtaaatgt cgcggaaagc
      841 gtcgcagacg gcatctttga cggtcagtcc gacaaattgg atgctcttaa cgttggcacg
      901 ctttgcctcg actttgactt tgaacgtctg ctgtaaagta aaccaattaa accagttgat
      961 atttttggcg agtttgtaga tgtcgcgctc attgcggtat gtccctttgg tcaggcgcag
     1021 caggatacgg ctggcagtac gcgaatgcag gttggcggcg taaacctgtt ccaatccgcc
     1081 ccggcaggaa acgccgccgt caaacacttg tacatcggta cagccgaggc tttcgagttc
     1141 ttgagataaa acggtctcca agccgcgcgg gcaggtggcg aaaagtgtat ataaagtatt
     1201 cataacgtgt cctttccgga cggatgttcc ggcaatggcg gattgaaagg tttattcggc
     1261 ggtaaatgcc gtctgaaatg tccggctgcc gattgtaatg tccgccgtaa aatcgcgcct
     1321 gccttcgaca cagatgggca ggcggatgcg tactgcctgc caagtccccg acggttgccg
     1381 ctcgaacata tagcggttga aacccatatc catatttttc atactgaagc tgatgctgac
     1441 ctgttccgtg ccggcgggcg cgtgttcgat ataaatatca aacggttttt tggttgaaac
     1501 ggcggcggcg cggacgcggc ttccgtccgg cagcgtgcaa ccctcggtca aatcgcattg
     1561 cgccgccaca gcttgcggct gctgcgcctg ccaccattgc aacaaaacga gcttgacggc
     1621 ggcaaaggca atcagcagca gtgcggcaag cagtaattta cggtttttat tcatgattct
     1681 tccctattgt ttcatccctt accaccacgt gcgcccccaa tccgagccat agttttccat
     1741 aacgtgcaac cgtctgtccg tttgctgcaa gtacgagcgg cacggatacg ccctgccgca
     1801 gggcttctgc cgcctcttgt gcctgaacat catcgcacac gcgccaaacg accgcatccg
     1861 cgcccttttc ctgcgcctgc cgccattgtt cgcgggtctg taccatcatc cacacgctgt
     1921 cggcgtgttc gggtctgtcc tgccattgcg ccgcctccat caaaacgttc gcaccgcttc
     1981 cctctgccga acccaaaggc aggacgcggt acgcgcccat actgttctca ccgtacaaac
     2041 ccgttttcag gctgccgtac aaacggcgcg ggacggacag cgaacgcaaa agcgcgccgt
     2101 tggcaggcag catgggggca acggtaaaat cacccgcctt ctgccaagac cattcctgcc
     2161 cttcgcggga ttgcggttcg cccatccatt ggtcggggtt tacccatagg aatttcaggc
     2221 agacgcgggc gtgttcgtag gaatggattt tagtcagcca aggcgtggcg gcgaggatgc
     2281 ggatgccgag ttcttcttca aactcgcgtt gcaaggcttg gaagtcggtt tcgcccgctt
     2341 cgaccttgcc gccggcaaat tcccaatatc cggcataggg tttaccttcg gggcgggaac
     2401 tgagcaggta gtcgccgtcc cggttgagca ggatgccggc aacgacgcgg ataaggggtc
     2461 gggtgtcttg ggtcatggga agggttggag aaggtcgtct caaaatccgg aaaggtcgga
     2521 ttcgggcatc cgacctgcca atgggtactg ttttattttt cggcaacggc aaacgccaat
     2581 acggtgtctt cttcgtcgct catgctgagg ctgacgcggc tgatgccttg ttcctccagc
     2641 catttggaca gggcgggggc atagaaaaat tcgggtttgc ccaatgcgtc gtgcccgatg
     2701 ccgatgttgc ggaaggaaac cgcgccgcgt atgcccgtac cgacggcttt ggcgaaggct
     2761 tctttggcgg caaagcgttt ggcaaggtag ttgacgggtt tgcccgcctg agggaattca
     2821 agcagttctt cgggggtgag gatgcgctcg gcaaacgcct gtccgaactt tttgctcagg
     2881 cggacgatgc gcttgaggga aacaatatct gtgcctatgc cgtaaatcat aatttgcgct
     2941 ccttcgccct tggttgcggg tagtgatgaa tgtgatgatg aatgccgtca atttcttggg
     3001 gcgttaaaat ggcgtgaata tccacttttg gggttttgtc ggtaacaatt ttcattgtaa
     3061 ccttttgcat ttgaaattct aaaacatcgt gccaattatt taataaggag ttaaggcatt
     3121 taataatgaa ttaaagtttg attaaacctg aatacccgat tttcagagcc aataccaata
     3181 aaacaataaa aattaacatt ttaaaaaaat ttgggctaat cttagtcctt aaccgaattc
     3241 caacatacaa tccaataaca gacaatacgg acagtaaaaa tattaaaccg tattcactct
     3301 tatttaataa ccaatactgg tctcttagca tatatatttg aacaattttt gccaaaagat
     3361 agcatagatt gcttgatttt gcgatacgat ttttattctc tgtttcgcta agcaaaaata
     3421 ttaacaatat gggagacatg gcattggttg aaccgccgat gatgcctgcc aaaaacccaa
     3481 aaagaaccat attcttatta ttggcaacta cttgaatatt ttttgctttt gcacatacat
     3541 ttaaaatacc attgacagaa taatacaatg taatgattgc catcagtaaa agcagccaag
     3601 acactggaag tatcaaaagc aacttcaccc ccaaaatgct gccaacgacg ctgccgatag
     3661 caagcaattt ataggttttt aaataataaa caatctcttg ccaaaaaccc tttttgttat
     3721 tgctgcatag aaccaacaag ctcattaaca ggcttggtaa tgccaccaag gcaacaacct
     3781 tagacaatgg catgataaaa gccaatgcgg ttgtaccgag catcggaaat cccatgcctg
     3841 taattccgtg cagtattgcg gcagcaacaa aaacgataga ttgcattatt tcttgcataa
     3901 ccgtccttta tacagtaatt cttgctctaa tataatgcct gtttttatcc gtataatcag
     3961 ttccggcatg caaaccattg acattgtcaa taataatcaa atcatcatct tgtgcagaaa
     4021 attcttgaat atcactcgca ttttttataa catttacaag ataatcaagt gcaagtacca
     4081 tttcctttga tactttattt ctattttctt caataccttt atagatacaa tcccttctaa
     4141 atctaatcat ttgagtattg accgataaga tattacccca tctcacacct tgttttttat
     4201 caaatgatgc tggtgtttta aatggatata aattgcccgt taatgttttt aagtgttttt
     4261 taccggtttc tgttttagat aactgattga caatatctga tgaacttaaa aatagggaat
     4321 tacctccatc attggctgat tttactacat acataaccaa ataactttcc gggttttcac
     4381 taaaagatga atcactatga agtggacatt ctccaacatc ttccgagaaa gttatatcat
     4441 tatttattac atagtcatcg ccgcgatatt taatatccaa aaatatagat ttaaaaccat
     4501 cgtctttgtg gtttgtcagc attcctaatt gagatagaaa gtcaaataat ttcttgtttc
     4561 tccggctgtc atcaagatgg tttagattaa gaccgcgaac aatacaatac ccaatactct
     4621 gtactgtatt gtgaatttct tctatcaaac tattattgtt tgcagataaa atatactttt
     4681 gattcataac atactcctgt cgtgaaatgc cgtctgaaac ggtttgcctg cccttacggc
     4741 agcagccttg ccctgaacat cgcctccttc atttggcgca cggcctcggg caatccgagg
     4801 aaaagtgcct gcgaaatcag cgaatgcccg atgttcagtt cgcggatggc gaggatttgg
     4861 gcgatggggg taacgttgtg tatggtcagt ccgtgtccgg cgttgacgac caagcccaaa
     4921 tcgctggcaa aatgcgcgcc gttttggatg cgctcgaact gcctgatttg ttcggcgtgg
     4981 ctgcgcgcgt cggcatacgc gcctgtgtgc agctcgacaa cgggcgcgcc gacatcacgg
     5041 gcggcttgga tttgcctgtc gtcggcatcg ataaacaagg acacgcgtat gcccgcgtcg
     5101 gtcaggattt tggtgaattc ggcaattttg tcttgttgcg ccaatacgtc caagccgcct
     5161 tcggtcgtga tttcctgacg tttttccggc acgatgcaca cgtcttccgg catcacttta
     5221 agcgcgtttt cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg cgtgcggatg
     5281 gcgtttttga cagcaaacac gtccgcgtct ttgatgtggc ggcggtcttc gcgcaggtgc
     5341 atggtaatca ggtcggcacc gtgcgtttcg gcaaccagtg ccgcctccac ggggctggga
     5401 taagtcgtgc cgcgcgcatt gcggacggta gcgatgtggt cgatgttgac acctaaaagc
     5461 ataatctttc cttttatttc tgccttcaga cggcatttga agccgtgccg tccgaagtcg
     5521 ggacggtttc ccgggcggtt tctttgcggt caaactgccg tatctgttcc aacacctgcc
     5581 gcgatttcag cccctcgggc aacaggtggc ggataaaaag ccgtgtgatt ttcaatgcct
     5641 gttgcaggct ttcggcagtg cggaaactgc cttcgcgcaa atcgatcagg ctctgcccgg
     5701 gggcgacaac gccggcggcg tgcggcggta cggcaaatcc ttttccgacg gggaagacgg
     5761 ctgtttccgg gcggacaagg tatgtgccgc ctgccgcaat cgtcccgccg tccccgtcgc
     5821 ggttcaaatc gggggcaacg cccaacaggt tcagcagccg ccactcgaaa cggcgcaagt
     5881 cgtcgatata agcggctttg cagcacaccg cctccatcac ttccgccaac gcgtcgtata
     5941 actcgggcaa cggatcttcg cggacggtca acttcagcat caactcgttc acatacaatc
     6001 cgctgaacaa agccctaccc tgcggctgcg gccacccgcc tatccattcg gcgcgatgta
     6061 gggtcttcaa ctcttgcgaa ccataccaag acgcactcac cagcacaaac ggcaccaaca
     6121 cgccgcgcaa ttcgctctgc cgtttgcgcg cgcttcttgc caaaagtgcc acacgcccgt
     6181 aacggcggct gaacgcttcg acccgcaggc tgctttcgcg ccagggcgag gaagccaaca
     6241 ggaagacggg ttcgtggttg actcggtgtt cggacatcat gcctgaaatg ccgtctgaac
     6301 gctgccgccg ctacaaaacg gcggtcgggt acgaaccgat gactttgacg aacgaagcgc
     6361 gttcgcccaa gcgttccaat gccgtctgaa tctgcgcgtc ccggcggtgt ccttcgatgt
     6421 cgatgaagaa caggtattcc cacaaaacgg atttactcgg acggctctca aacttggtca
     6481 tggaaatacc cgactcagtc agcggttgca gcagcgaggc aaccgcgcct gcccggttgg
     6541 gcgcggaaac ggccagcgaa gtcttgtcgc tgccgcttgc accggtttcg tgatgtccca
     6601 tcaccaagaa gcgcgtggtg ttgttcggtt cgtcttcgat gcactcggca accatatcga
     6661 gtccgtagat ttccgccgcc gtgcgtccgg cgatggcggc aaccgtaccg tcgtccgatt
     6721 cggcaaccag ccttgcggct tcggcattgc tggacacggc aatccgttcg gcgttgggca
     6781 ggtgtctgcc caaccagtcg ttgcactgcg ccaacgcctg cgcgtgggaa aagactttgg
     6841 caatgccttc ggtgctgccg ttgtttttac gcaaaaggtt gtggtggatg cgcaaaacga
     6901 cttcgccgca cgcctgcaac gcggtaacgg caagcaaatc caacgtgcgc ccgaccgagc
     6961 cttcggtcga gttttccacg ggcgcgacca gataatccgc ctgacgggtt tcaacctgct
     7021 tgaagcagtt gtctatggtc ggacacgcca tggtgtgcgc ggcgtgtccg aaatgcttga
     7081 tcgccgcctg ctgggtaaac gtgccctgcg gccccagata ggcgatggtc agcgggcgtt
     7141 cgacggcgag gcactcgctc atcacttccc gaaacaggcg tgctaccgat tcgtcgggca
     7201 gcgggccttt gttcaaatcc tgaatgcggc gcaacacggc gacttcgcgt tcgggacggt
     7261 acactgcgcc cgtgcctttc agctcgccga tggcgtgggc gtgttgcgca cgttcgttga
     7321 gcaggcgcag gatttcggca tcgatggtgt cgatggcgtt gcggtgggga aggaggagtt
     7381 cgtcgatagt ttgggacata atttcgcctg aacggtggtt tgccgtgcat tctaacatca
     7441 aatgccgtct gaaacggttt cagacggcat tttccgttaa aggtattcgc tttgcccgtt
     7501 ttctttccac gcacgatgcg agcagagcca caaaagcgca atgccgcaag aggtagacgc
     7561 ggtcatggtt gccgccatca cggttgccga accgtcgtgc aagaaggtcg ccgccatacc
     7621 cacccccgcg ccgattaaag attggaatac acccaatacg gcgtttgcgc tgccgccctc
     7681 ttctttgaaa taggacataa aacacgcctg cgtgtttgca ccgaccaagc cctgcgtacc
     7741 gacggaaaac atcacgcacg cgaccagcag ccaaaacggg ggcaacccga aaaacagcac
     7801 ggcggcgagt tgggacaggt tggcggcaaa ctggacgaca atcccccaca gcaggatgct
     7861 ttgcggatgc acgccggttt tgagccgcca cgcggtaata cggttgaaaa acatcatcgt
     7921 gatgatgttg agtgcaaacg cccaagcgta ctggtgcggc gtaacgtggt agagctgctg
     7981 gtacacgaag gaagattcgg tcagaaaggc gaacatcgaa ccgaagctga atgcctgaaa
     8041 aaacagataa cccatcgcgg cacgggtttt caatacgcgt ttgaaccgcc cagccaccag
     8101 cccgaacaca tccctgccga ttttgccgcc gacggcgggc ttgggcagga aatactgtac
     8161 caaaccgagc agcaccagcg aatacgccgc cagaaaaaca aaaatcgcct gccagccacc
     8221 caagccctgc aacaatgcgc cgaccatggg tgcgaccagc ggcacaacca tcaaaatgat
     8281 gccgataagg gcaaacatct gggcggcttt gcgtccggaa taataatcgc gcaccattgc
     8341 gccgacgatg accacagtca tgcccgcacc gaatgcctgc acgacgcgca ggttgaggag
     8401 ctgttcggca ctcgaagcaa atacgatggc ggcaacggca aggcagtaga cggccagtcc
     8461 ggtcagcgcg acgggtttgc gccctttgat gtcggacacc gaaccgccga ccacctgtcc
     8521 gaacgccgtg ccgaacataa acaaactcag gctctgttcg atgcggtgga catccgcgtt
     8581 cagcgactgc gccatttcgg gaatcgcggg caggtaggca tcgatggaaa acggcatcag
     8641 cgtaaccagc atcgccatca aaaccgccat cagtttttcg ctcatttcag gataatgggc
     8701 agaaggcata aattctcgtc cgattcgcaa acaaaccgcc cggcggcgga aaacacaata
     8761 cgcggaaacg gaaaatttta acacaccggc acaaacaggc ggcaacaaaa gccccggcaa
     8821 aaccggacga accggaacga tatagtggat taaca
//
LOCUS       DAWKQB010000082         8740 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.082, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000082 DAWKQB010000000
VERSION     DAWKQB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.082"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            213..1748
                     /locus_tag="WJL14_001385"
     CDS             213..1748
                     /locus_tag="WJL14_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7656446.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRRHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            1745..1837
                     /locus_tag="WJL14_001386"
     CDS             1745..1837
                     /locus_tag="WJL14_001386"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ7656447.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(1873..3117)
                     /gene="lysA"
                     /locus_tag="WJL14_001387"
     CDS             complement(1873..3117)
                     /gene="lysA"
                     /locus_tag="WJL14_001387"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ7656448.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFENYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLIEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYARAVQKLMGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIAPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGSSMASNYNTRNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(3128..3298)
                     /locus_tag="WJL14_001388"
     CDS             complement(3128..3298)
                     /locus_tag="WJL14_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ7656449.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QSKPQSASQTQK"
     gene            3369..3692
                     /gene="cyaY"
                     /locus_tag="WJL14_001389"
     CDS             3369..3692
                     /gene="cyaY"
                     /locus_tag="WJL14_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ7656450.1"
                     /translation="MMTESEFIRMSEELFEHIEDQIDENGWDFDCQFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     KIAEL"
     gene            3719..4735
                     /locus_tag="WJL14_001390"
     CDS             3719..4735
                     /locus_tag="WJL14_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ7656451.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTALLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            4761..5178
                     /locus_tag="WJL14_001391"
                     /pseudo
     CDS             4761..5178
                     /locus_tag="WJL14_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
     gene            5200..5706
                     /gene="luxS"
                     /locus_tag="WJL14_001392"
     CDS             5200..5706
                     /gene="luxS"
                     /locus_tag="WJL14_001392"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ7656452.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVVNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            5852..8668
                     /gene="polA"
                     /locus_tag="WJL14_001393"
     CDS             5852..8668
                     /gene="polA"
                     /locus_tag="WJL14_001393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ7656453.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QEAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIRNKNTNARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VDGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2061 a         2263 c         2294 g         2122 t
ORIGIN      
        1 gctaatctag tacagcccct aaaatttttc gttttcaaac cttcaccgct tgccatcagc
       61 gttaaatttt tttacgataa gcacatagat tgtaaacaat cggtcacaag ccggtttgtt
      121 ttttcagaag acattatccc tgtcagacgc tgtttctata tatgtttgcc tacaacggct
      181 tgtttttaat aaataactca agaggtatca acgtgtctga ttccaaaacg aaagaacgcg
      241 ccacattcgg cacgcgccgc gcgtttatga ttgccgccat cggatccgcc gtcggcttgg
      301 gcaatatttg gcgtttcccc tatattgctt ttgaaaacgg cggcggcgcg ttcatcctgc
      361 cctatctggt cgcgcttctg acggcgggca ttccgctgct gctgctcgat tatgccatcg
      421 gccaccgtta ccggggttct gcgcccttgg ctttccgccg cctcggacgc tggtttgagc
      481 cggtcggctg gtggaacgtg atgaccaata tcgtcatctg catctattac gcagtgatta
      541 tcggttgggc ggcaagctat acctattatt cggtcaacgc tgcctggggt gcggatccgc
      601 agggtttttt ctttaaggac ttcctgcaaa tggcgggccc ggaagccttg ggtttggatt
      661 ttgtcggcaa agtcgccggt cctttggcgg gcgtgtgggt ttttaccgcc gccattatgg
      721 ctttgggcgt gcaaaagggc gtggcgcgcg cctcgtcgtt ctttatgccg ctgcttttgg
      781 tgatgttttt gattatggtc ggcatttcgc tgacgctgcc gggcgcggca aagggcttgg
      841 acgcattgtt tacgcccgac tggtcgaaac tcgccgattc caaggtctgg gtggcggcat
      901 acgggcagat tttcttttcg ctttccatct gcttcggcat tatggttacc tattcttctt
      961 acttgaagaa gaaaacggat ttgggcggca cgggcttggt ggtcggtttt gccaacagca
     1021 gctttgaact gctcgcgggt atcggcgtgt ttgccgcatt gggctttatg gcgcaggcgg
     1081 gcggtaaggc ggtcaacgag gttgcctccg gcggcatcgg tttggcgttt atcgcctttc
     1141 cgaccattat caaccaggca ccgatgggct ggctgatcgg catattgttt ttcggttcgc
     1201 tggtgttcgc cggcgttacg tcgatgattt ccatccttga agtgattgtg gcggcgattc
     1261 aggacaagct gaacatcggg cgcgtcaacg ccacgctgct ggtctgcatt ccgatgggca
     1321 ttgtttccac gctgctgttc ggtacggcga cggggctgcc ggttttggac gtgatggaca
     1381 aattcgtcaa cacctacggc attgttgccg ccggctttgt ttatgttgcc gccatcatca
     1441 tcagcggcag gctgccggaa ttacgcaggc acctgaatgc cttgtcctcc atccgcatcg
     1501 gcggcttgtg gacggtctgc gtcgtggtta ccgtcgtaat gctcggctat atgctgttta
     1561 aagataccag cggcctgatg gagaaaaatt acgaaggtta tccggatggt ttcctcagta
     1621 ttttcggctg ggggatgtcg gcggcgttgg tcgtgttcgg gctgctgctg tcgttgctgc
     1681 cttggaaaca cggtcaggat ttcaacgtca aagacgaaca cgaacatgaa caaggagacg
     1741 aaaaatgagt acttccgcca ttgtgatgat gattgtctca atcgtgataa tctggggagg
     1801 gctgctgctt tccctgttaa ggctgccgaa cgagtaagcc tttagagcgt taaaaatgcc
     1861 gtctgaactg cttcagacgg cattttgatg ttcggcttgc aggcaggcga gttcgtttgc
     1921 catttgctgt tctaaggttt cgcgtcggcg gatgagtcgg tattcgttgc cgtcaaccaa
     1981 tacttccgcc gcacggttgc gcgtgttgta gttgctcgcc atgcttgagc catacgcacc
     2041 ggcgctgcgg ataagcagca aatccccttc ttcgcaggcg atggtgcggt ctttgccgag
     2101 gaagtcgccg gtttcacaaa tcgggccgac gatgttggcg gtcagcggcg cgatgttttt
     2161 ggtttcaacc gcttcgatgt ggtggtaggc atcgtatagg gccgggcgca ttaaatcgtt
     2221 catcgccgca tcgaccatga cgaagttttt ctcttcgccg tgtttgacaa actcgacgcg
     2281 cgtcagcaac gcgcctgcgt tgccgaccaa gctgcggccg ggctcaagaa tgagtttcag
     2341 acggcgtgtc cccatcagtt tttgaaccgc ccgggcatac gcgcccaagt cgggcacatt
     2401 ttcgtcttgg taaacaatgc cgacgccgcc gcctaagtcc aaatgttcca aaacaatgcc
     2461 ttcggcggca agtgcgtcaa ccagaattaa aatgcgttcg caggcttcga taagcgggct
     2521 taagtcggtc agttgagaac cgatatggca gtcgatgccg atgattttca aattgggctg
     2581 ttgtgcggcg tggcggtagg cttcgagcgc gtcggcgtag gcgatgccga atttgttggc
     2641 tttcagacct gtggagatgt agggatgggt tttggcatcg acatcggggt tgacgcgcag
     2701 ggagacgggc gcggttttac ccaaacgcgc ggcaactttt tgaatacggt cgatttcggg
     2761 aatgctttcc atattgaagc atttcacgcc cgcattcagg gcaaactcga tttccgcctc
     2821 gcttttgcct acgccggaaa aaatcgtttt cgccgcatca ccgccagccg ccaaaacgcg
     2881 tgccaattcg ccgccggaca caatgtcaaa accgctcccc aaagaagcaa agtgtttgat
     2941 gatgcttagg ttgccattgg ctttaaccgc atagcagacg agcggggaga gctcggcaaa
     3001 ggcagtttga tagttttcaa atgcttcggt cagcgcggat tggctgtaca cataaagcgg
     3061 cgtgccgaat gcttcggcaa ggcgggggta ggggacttgt tcgcaaaata gggtcatgtt
     3121 ttcgttttca tttttgggtt tgtgaagcgg attgcggttt gctttgaagt tgcaaaccgg
     3181 tttggattac gccgaaacgc gccttgtcgc cttctttggg caggtagaga tcgcctttgt
     3241 aaccgcaggc cgagagcagg agggcggttg ccgccgcaaa aaatacgccg tatttcatcg
     3301 gtaaacttcc ttcataagcg cgaatgtggc aagattcggt atcttaaaca aaaaacacgc
     3361 aaaaagccat gatgaccgaa agcgaattca tccgcatgag cgaagaattg ttcgaacaca
     3421 tcgaagacca aatcgacgaa aacggctggg atttcgactg ccagtttgcc ggaaacgtcc
     3481 tgaccatcga agccggagac ggcacgcaaa tcatcgtcaa ccgccacacg cccaatcagg
     3541 aattgtggat tgccgcaaaa agcggcggct accatttcgc cgagcaaaac ggcaaatggc
     3601 tggcaacgcg agacggacga gatttttatg acgttttaaa cgaagccctg agcgcggctt
     3661 cgggcgaagc ggtaaagatt gccgaattgt aatttgagtg ttatcacgga gagaaaaaat
     3721 gaacacacgt cccttttatt tcggacttat atttatcgcg attatcgcta tacttgctaa
     3781 ctatttagga aacactgatt tttcccatca ttatcatatc agtgctttaa ttattgctat
     3841 cttgctggga atggcaatcg gcaataccat ttatccgcaa ttttcaacac aagtggaaaa
     3901 aggcgttttg tttgccaaag gcgcgcttct tcgcactggc attgtgctgt atggtttccg
     3961 cctcactttt ggcgatattg ccgatgtagg attaaatgct gtcgttaccg atgcaatcat
     4021 gctaatttca accttctttt ttaccgcact tttaggcatt cgttatctaa aaatggataa
     4081 acaattggtt tatctcactg gggcaggttg cagcatttgc ggtgcggcag cagtgatggc
     4141 ggcagagcct gttaccaaag cagaatccca taaagtttca gtggcgattg ccgtagtggt
     4201 cattttcggg acgcttgcta tttttactta ccccttgttc tacacgtggt cacaacattt
     4261 aattaacgcc catcaattcg gtatttatgt tggttctagt gtacacgaag tggctcaagt
     4321 ttatgcgatt ggggaaaata ttgatcctat cgtggcgaat actgccgtca tttccaaaat
     4381 gatccgagtg atgatgctcg cccccttttt attaatgctt tcttggttat taacacgtag
     4441 taatggagta tcagaaaata catcacacaa aattacaatt ccttggtttg ctgtactttt
     4501 tattggtgtt gccattttta attcttttga tttattacca aaagaactcg tgaaattatt
     4561 cgttgaaatc gattctttct tattaatttc atcaatggct gcgcttggct taacgacgca
     4621 agcaagcgca atcaaaaagg caggattaaa accgtttgtt ttaggaatac taacttattt
     4681 atggctagtg gttggtggat ttttagtgaa ctatggaata tcaaaattaa tataaaattc
     4741 actaaagaga gcgttaccca atggcacaat taccgctata tctgacttct gaaatcaaag
     4801 actttactgt cggcacgcct aaagttttgg aatcattttc caaacatatc cttatggtgt
     4861 tgtctttgaa gacgacggcg acacgggcta cttctatgcc gcttcgcaag acgggattat
     4921 agatgccttg cacatctata atgtcgaaga tgtatccgac aaacatatcc caatcatgtt
     4981 ttgattttat gggatgatgc ctgcaccata gccgcattgc gtatcaacga ctacattcat
     5041 gccgtctatg attttgtcgc gcaggcagga tattgccgca acggcttccc tgaagcaggc
     5101 ggcgaatggg tgaaagtcga aaaccgcatc ttggacgatg aattgctgga caaaatccta
     5161 tcccgaaaat ctacataacc tactcaaaag gataacacga tgcccctgtt agacagtttc
     5221 aaagtcgacc acacccgtat gcacgccccc gccgtacgcg tggcgaaaac catgaccacg
     5281 cccaaaggcg acaccattac cgtgttcgac ctgcgctttt gcattcccaa caaagaaatc
     5341 ctgcccgaaa aaggcataca cacgctggag catttgttcg caggttttat gcgcgaccac
     5401 ttgaacggca acggcgtcga aatcatcgac atttccccga tgggctgccg caccggtttc
     5461 tacatgagcc ttatcggtac gccttccgaa cagcaggtcg ccgatgcgtg gctggcttcg
     5521 atgcaggatg tggtcaatgt caaagaccaa agcaaaatcc ctgagttgaa cgaataccaa
     5581 tgcggcactt atctgatgca ctcgcttgcc gaagcgcaac aaatcgcgca aaacgtgttg
     5641 gcgcgcaaag tggcggtgaa taaaaacgaa gaactgacgc tggatgaagg gctgctgaac
     5701 gcctaatccg ccaaaaatgc cgtctgaaca agggtttcag acggcatttg ccttttccgt
     5761 tataatccgg ggttgtgcgg gggcgagttt taagccggca tcgtccttcc ctattttttt
     5821 ctgtccctta tcggttttaa gcgggttttt tatgtccaat agacctacac tcctcctcgt
     5881 tgacggctcg tcctacctct accgtgcgta tcacgcgatg gggcaaaacc tgaccgcccc
     5941 cgacggcgcg ccgacgggtg cgctgtatgg cgtattgaat atgttgcgcc gtttgcggtc
     6001 ggaatatccg cacgattatt gcgcggtggt ttttgatgcg aaaggcaaaa atttccgcca
     6061 tcaaatgttt gaagaataca aggcgacgcg cccgccgatg cccgacgatt tgcgcccgca
     6121 ggcggaagca ctgccggatt tagtgcgcct gacgggctgg ccggtattgg tgattgggca
     6181 agtggaggcg gacgatgtga tcggcacgct ggcgaaacag ggggcggaac atggtttgcg
     6241 agtcattgtt tcgaccggcg ataaggacat ggcgcagttg gtggatgagc gcgttacgct
     6301 ggtgaacacg atgagcggag aaacgctgga tattgaaggc gtgaaggcaa aattcggcgt
     6361 gcgccccgac caaatccgcg attatctcgc gctgatgggc gataaggtgg acaacgtgcc
     6421 gggcgtggaa aaatgcggcc cgaaaacggc ggtgaagtgg ctggaagcct acggctcgtt
     6481 gcaaggcgtg atagaacacg cttcggaaat caagggcaaa gtgggcgaaa acctgcaagc
     6541 cgcgctgccc caactgccgc tgtcgtatga tttggtcacg attaaaaccg atgtggactt
     6601 gcacgccgag ctttcagacg gcatcgaaag cctgcgccgt accgcgccga aatgggcgca
     6661 actggttgtc gatttcaaac gctggggctt ccgcacctgg ctgaaagaag cggaatcaaa
     6721 catgaatacc ggctcgaccg atgatttgtt cggcagcgac agcatcggcg agcaggcggc
     6781 tttgaatgcg gaaatgccgt ttgaaaaaca agccgaaaaa gccaccgccc ccgaaaaact
     6841 ggattatcaa gccgttacca ccgaagcgca gtttgccgct ttgttggaca aactgtcgcg
     6901 ggcggacaca atcggcatcg atacggaaac cacgtcatta gacgcgatga acgcctcgct
     6961 ggtcggcatc agcatcgctt tccaagcagg cgaagcggtt tacatccccg taggacacag
     7021 cctgaccgcc gcacccgaac agcttgattt gcaagacgtg ttaggccgtc tgaaaccgca
     7081 tttggaaaac cccgccctga aaaaaatcgg gcaaaacctc aaatatgacc aacacgtttt
     7141 cgccaattac ggcatcgccc tgaacggcat cgccggcgac gccatgctcg cttcctacat
     7201 cattgagagc catctcggac acggcttgga cgaattgtcc gaacgctggc tgggcttgga
     7261 aaccattacc tacgaatcgt tgtgcggtaa aggcgcgaag caaatcggtt ttgccgatgt
     7321 cgccatcggg caggcgaccg aatacgccgc ccaagacgcc gatttcgccc tgcgcctcga
     7381 agcgcacctg cgcgcacaaa tggacgaaaa acagcttgaa atgtatgaga aaatggagct
     7441 gcccgtcgcg caggtattgt ttgaaatgga acgcaacggc gtgcaaatcg accgcgccga
     7501 actcgcccgc caaagcgcag aactcggcgc cgagctgatg aagcttgaac aagaagccta
     7561 tgccgccgca ggacagccgt tcaacctcaa ctcgcccaaa cagcttcaag aaatcctgtt
     7621 cgacaaaatg ggcatcccca ccaaaggcct gaaaaaaacc gccaaaggcg gcatttccac
     7681 caacgaagcc gtgctcgaac agctcgcgcc cgactacccc ctgcccaaaa tcatcctgca
     7741 aaaccgcagc ctggcaaaac tcaaatccac ctacaccgac aaactgcccg aaatgatttc
     7801 ccccaaagac ggccgcgtgc ataccaccta cgcccaagcc gtcgccatta caggacgctt
     7861 ggcaagcaac aaccccaacc tgcaaaacat ccccatccgc accgccgaag gccgacgcgt
     7921 gcgccgcgcc ttcaccgcac cgcaaggcag cgtcatcgtt tccgccgact attcccaaat
     7981 cgagctgcgc atcatggcgc acctctccgg cgacaaaacc ctcattgccg cgttccaaaa
     8041 cggcgaagac gtacaccgcc gcaccgccgc cgaagtgttc ggcatcgccc ccgaaaacgt
     8101 ctcgtccgaa caacgccgct acgccaaaac catcaacttc ggcctgattt acggcatggg
     8161 ccaatacggc cttgccaaat cattgggcat cgacaacatc tccgctaaaa actttatcga
     8221 ccgctacttc gcccgctacc ccggcgtcgc cgaatacatg cagcgcacca aagaacaagc
     8281 cgccgcccaa ggctttgtcg aaaccctgtt cggcagaagg ctctatttgc ccgacatccg
     8341 caacaaaaac accaacgccc gcgccggagc cgaacgcgca gccatcaacg ctcccatgca
     8401 gggcaccgcg tccgacctca tcaaacgcgc catgatagac gtgtcccgct ggctttcaga
     8461 gtgcgaagcc tccccgtggg acgaactctt acaaagcaaa ctgattatgc aggtgcatga
     8521 cgaactggtg ctggaagtcg ttgaaaccga actggatttt gtcaaagaaa aactgccgca
     8581 gattatggcg aaagtggacg gcggattatt ggatgtaccg ctggtggctg aggttggcgt
     8641 aggggagaat tgggaagagg cacattgatt gaaaggtgtt atatgctatc tttatttaaa
     8701 taaaatttaa tttttggtat attttttcta aatgttccta
//
LOCUS       DAWKQB010000083         8738 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.083, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000083 DAWKQB010000000
VERSION     DAWKQB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.083"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(276..1367)
                     /gene="ychF"
                     /locus_tag="WJL14_001394"
     CDS             complement(276..1367)
                     /gene="ychF"
                     /locus_tag="WJL14_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ7656454.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(1482..3158)
                     /locus_tag="WJL14_001395"
     CDS             complement(1482..3158)
                     /locus_tag="WJL14_001395"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ7656455.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGKLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLADLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALAKGLPNLLKHISNLKNVFGLPIVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            3333..3764
                     /locus_tag="WJL14_001396"
     CDS             3333..3764
                     /locus_tag="WJL14_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7656456.1"
                     /translation="MSSNAWLFWALASAGFASLTAIFAKMGLQGIDSDFATFIRTLVI
                     LAALLLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(4010..4705)
                     /locus_tag="WJL14_001397"
     CDS             complement(4010..4705)
                     /locus_tag="WJL14_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ7656457.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4723..5223)
                     /gene="hpaC"
                     /locus_tag="WJL14_001398"
     CDS             complement(4723..5223)
                     /gene="hpaC"
                     /locus_tag="WJL14_001398"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ7656458.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRSLD"
     gene            complement(5338..5778)
                     /gene="nadR"
                     /locus_tag="WJL14_001399"
     CDS             complement(5338..5778)
                     /gene="nadR"
                     /locus_tag="WJL14_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ7656459.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(6121..6630)
                     /locus_tag="WJL14_001400"
     CDS             complement(6121..6630)
                     /locus_tag="WJL14_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ7656460.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPDEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(6709..6784)
                     /locus_tag="WJL14_001401"
     tRNA            complement(6709..6784)
                     /locus_tag="WJL14_001401"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6749..6751),aa:Lys,seq:ttt)
     gene            6995..8074
                     /gene="apbC"
                     /locus_tag="WJL14_001402"
     CDS             6995..8074
                     /gene="apbC"
                     /locus_tag="WJL14_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ7656461.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIAKIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         2053 a         2480 c         2232 g         1973 t
ORIGIN      
        1 aagttaatcc actatactcg aaacgcggcg gcgcaaatgc cgtctgaaag gtcattttgt
       61 atcggggctc gggatattcg gaatgccgga cggctttttg caacagcggg gcaggcggtt
      121 tgttttgcag gaatcgggga gggcaaatca gaaatgcggg cgggatttta ttttgatgca
      181 gccgcattcc ggcggtatgg aaacgccgaa agccaccaaa accgacttca gacggcattt
      241 tcggcaaagc cgtctgaaaa cctgccgcat ttgggttaca cgttaaacaa aaagtgcatc
      301 acatccccat cctgcacgac gtattccttg ccttccacac gcattttgcc ggcttctttg
      361 gctttggctt cgccaccgag cgagacaaag tcgtcgtaag aaatgacttg ggcgcggatg
      421 aagcctcgtt caaagtcggt gtgaatcacg ccggcggctt gcggggcggt gtcgcctttg
      481 tgtatcgtcc acgcgcggac ttctttcacg ccggcggtga agtaggtttg caggcccaag
      541 aggtcgtagc cggcgcggat caggcggttc aagccgggtt cttccaagcc catttcggcg
      601 aggaactcgg ctttttcgtc gtcttccaat tcagcaattt cgctctccat cgcggcgcat
      661 acggcgacga cgggggcgtt ttcttttgcc gccaattctt tcaggcggtc gaggtgcggg
      721 ttgttttcaa aaccgtcttc ggcaacgttg cccacataca tcgccggttt ggcggtcagc
      781 aggaacagcg gtttgagcat cgcgcgttct tccgcgtcca aaccgaagga acgcacgggt
      841 ttgccttcgt ccagatgcgg cagcagtttt ttgcacaaat cgaccagctt ttgcgcgtct
      901 ttgtcgcctg aacgggcgcg tttttcttcg cggacgatgg ctttttccac acttgccaag
      961 tcggcaagcg ccaactctgt accgatggtt tcgatgtcgg caatcggatc gactttgccg
     1021 gaaacgtgga cgatgttgtc gtcgtcaaag cagcgcacga cgttgacgat ggcatcggtt
     1081 tcgcggatgt tggcaaggaa ctggttgccc aagccctcgc ctttgctcgc gcctgcaacc
     1141 aaaccggcaa tatcgacaaa ttcgacgatg gcaggctgca ttttttgcgg attgacgatt
     1201 tttgccaatt cggccatacg cggatcgggg acttcgacga tgccgacgtt gggttcgatg
     1261 gtacagaaag gatagtttgc cgcttcgata cccgattggg tcagcgcgtt aaaaagggtg
     1321 gatttgccga cgttgggcaa accgacgatg ccgcatttca aactcatgtt ttttcctgaa
     1381 aatagagaaa tttaacggcg gattatagca taccgccgcc cgcgttccga aaaaatgccg
     1441 tctgaaacgg cttcagacgg catccggttt cagaaaaccg ttcagaacaa gccgtgaatc
     1501 acgccttctg cgtccacatc gattttctcg gcagccggaa ctttgggcag gccgggcatt
     1561 ttcatcatgt tgccgcacag ggcgacgatg aaacctgcgc ctgcggaaac agtgataccg
     1621 cgcacggcga tgcggaagcc ttcggggcag cccaacagtt tggcgttgtc actcaaagag
     1681 tattgggttt tcgccatgca gatcggcatt ttgtccaagc ccagtttttc cagtgaagcg
     1741 atttcggcag acgcttccgc gctgaaatca acatcttccg cgccgtacac tttttgggca
     1801 atcgcacgga ttttgtcttt gatgcccaac tcgacatcgt aggcgaaacc gaagttattg
     1861 gtttgacttt caatggcgtt gacgactttg cgcgccaaat ccgcgccgcc cgcgccgcct
     1921 ttgccccaca cttcggtcag ggaaacttca acgccgtgtt cggcacaggc tttttcaatc
     1981 atcgccaact cggcatcgga gtcggacacg aagcggttga gcgcaacgac gatgggcagt
     2041 ccgaatacgt ttttcaggtt ggaaatgtgt ttcagcagat taggcagacc ttttgccaaa
     2101 gcgtctaaat tttcttcgcc gaggttggcg cgttccacgc cgccgttgta tttcaaagcg
     2161 cggacagtag ccacgacaac agccgcatca ggttttaaat ccgccagacg gcatttgata
     2221 tcgcagaatt tttccgcacc caaatccgcg ccgaagcctg cttcggtaac ggcgtagtcg
     2281 gcaaggtgtt tcgccagacg ggttgcggtt acggagttgc agccgtgggc gatgttggcg
     2341 aacgggccgc cgtgtacgaa ggccggagtg ccttcgatgg tttgtaccaa gttgggctta
     2401 atcgcatctt tgagcagggc cgccatcgcg ccgtgtgctt tcaaatcttt ggcgtaaacg
     2461 gggctgccgt ctttggcgta ggcgacaagg atgttgccca aacgctcttt caaatcgctg
     2521 atgtctttgg caaggcagaa taccgccatc acttcggaag caacggtaat gtcgaaaccg
     2581 tcaggacgca tcacgccgtc aacaggtttg cccatgccgt cgatgatgtt gcgcaactgg
     2641 cggtcgttca tatcgaccac gcgccgccac agcacgcgtt tcggatcgat gttcaactcg
     2701 ttgccttggt agatatggtt atcgagcatg gcggcgagca ggttattcgc cgcaccgatg
     2761 gcgtggaagt cgccggtaaa gtgcagattg atgtcttcca tcggcaaaac ttgggcatag
     2821 ccgccgcctg ccgcgccgcc tttcacgccg aacaccggcc ctaaagaagg ttcgcgcagg
     2881 gcaatcacgg catctttgcc gatgtggcgc aacgcgtccg ccaaaccgat ggttacggtg
     2941 gttttgcctt cgcccgccgg agtcgggttg atcgcggtaa ccaaaatcaa tttaccttgt
     3001 ttttgcggca gtttgaacgc ttcggcagga ttgattttcg ccttgtaatg accgtaaggc
     3061 tcaatgttgt cggcattcag accaagcttg gcggcaattt cgccaatcgg gcgcatggtg
     3121 gaggattggg cgatttcggc atcggttttg aagctcatga ttttccttta gaaatgagga
     3181 gggacatgcc gtctgaaagc atcaggcgac aaacaggtgg attgaaaata atatcaggca
     3241 tattataacg ttatccgcac caaacccgca gtgaaatttt tgacgctgca acaaaaatac
     3301 cgttcatatt gttcacaatc caaggagaaa acatgagcag caacgcatgg ctgttttggg
     3361 cattggcatc ggcaggcttc gcctcattga ccgccatctt cgccaaaatg ggtttacagg
     3421 gtatagattc agatttcgct accttcatcc gcaccttggt catcctcgcc gctttgttat
     3481 tgtttttaac ctacaccggc aaatggcagg gcgtgaacgg ctttacgggg cacaactgga
     3541 cattccttat cctatccggc cttgctaccg gcgcatcttg gcttgcctac ttcaaagccc
     3601 tgcaactggg taacgcctcg caagtcgccc ccgtcgacaa attcagcctg gtcttggtcg
     3661 cgctgatggc ggtggttttc ttggacgaac gcccgaacac gcaggaatgg ataggcttgg
     3721 ggctggtaac ggcgggcgtg ttggtgctgg cgttgaaacg ttaaaccgaa tccgccatac
     3781 cgcctgaaac cgggttttga caatctgacg ggcggcggtc tgaacaaagt tacccaataa
     3841 aatccgacac cgcgccgacg gcaaaccgcc gcatccgaca ggggtcggct tcagatggca
     3901 tttgttggac ggaaagcccc gaacgcccta ttttcacgcc tccacgaaaa tatgccggac
     3961 atccggtcgg aaccgttcgg cagccgactc ggcaagccac cgttttattt cacttccaag
     4021 cccctgcaag ggcttgagcc tctttcagac ggcataccgt gccgacatcc agccacaagc
     4081 ccgtatgctt ctgtccgctc acgcggtttt gccgcatttc gccccgcaat acgggcgcga
     4141 gtttcgccac actgcccgct tcgattccgt caaacatttc aggacggtaa atacccacgc
     4201 cgctaaatgt caatccgttg ccgccggata cttccgactg tacgctgccg tccggcagca
     4261 gggaaaaatc gccgtcaggg ttgtgcggcg gattttccac cagccacaga tgggcggaaa
     4321 tatgttccgg cagggacgat gccgtctgaa acgcggcggt aaaatcgatg tcggtcagca
     4381 cgtcgccatt gacgaccaaa aacggggtat cgcccaacag cggcaatgcc cgtgcgatac
     4441 cgcctgcggt ttccaagccg ccttcaggtt cgggcgaata ggcgatgttc acgccataag
     4501 ccgagccgtc gcccaaagca tcttctatct gccgacctaa ccaagcatgg ttgataacga
     4561 tttcggtaaa ccctgcctgc ttcagacggc ataggtgcca accgattaga ggcttacccg
     4621 ccacatcgag cagcggcttc ggagtggtat cggtcaaagg acgcatacgc tcgccgcgtc
     4681 ctgccgccag tatcatcgct ttcatatata tctgtccgaa tatcagtcta aagatctaaa
     4741 ctgccgtctg aaataaagca gcgcggggcg tttgcacccg cagtttttga tttcgtcgag
     4801 cctgacgtaa aacacaaaat gcgtgccgat ttcatgtttg ccgacaatat gcccgtgcag
     4861 gtgcgccaac gcgccttcta tttcaagttg tcccgttttg ccgcgatgcc agatgtggta
     4921 ggcaaaccgc tcttcgggcg acaggccggt cagcccggca aaatgttcgg caacatcctg
     4981 atgttcgtcc gccagcgtat tgatgcagag gctgccgttt tccgacagga tcggaatgat
     5041 tcgcgcactc cggttgatgc acagcatcac ggtcggcggc tcgtcggtaa ccggcgcaac
     5101 cgccgtcatt gtaatgccgt aacgccctgc cgcaccgtct gtcgtgatga catgaacgcc
     5161 tgccgcacag gatgccatcg catcacggaa cgaagtttga aaagttttct gcaaatccgc
     5221 catttttccc ctttaaactg tcccctatat aagaatgctg cacacaaggc accccccatg
     5281 tgcagcagtt ctgattcaaa aagccgtcgg tcggacattt ccgcgcgtta cggcgtatta
     5341 cgagttcaac gcatcctcga ttttggcaag ttctgccaac aggtctttaa gcagcagcat
     5401 tttctcgcgg cccagcactt cctcgatagc gtcgtaacgc tcgtccactt cttcgccgat
     5461 ttcctcatac agcttctcgc cctcggcagt cagcttcaga aaaacacgtc gttggtcgtt
     5521 ggaaggtttc aggcggacaa ccaaacccgc tttttcaagg cgggtcagga taccggtcag
     5581 gctggggcgc aaaatgcacg cctgattcgc caaatcttga aagtccagcg tgccgttttc
     5641 cgccaaaaga cggataatcc gccattgctg atcggtaata ttcgcctgat tcagaatagg
     5701 cctgaattgg gtcatcaggg cttcccttgc ctgtatcaga ctgatattga tagacgcatg
     5761 ttttgattgg gtaggcattg ttcaagtctc caagttatcg aaaatcaaac tttcaaaccg
     5821 tcgggaaagc ctgtgggcgt aaattttgat gcaaccgtta tataacaaaa cgaacatata
     5881 gcaacaatac gctataaacc gcatcggacg actgggtata aaagacttta attccgataa
     5941 tcctatctaa aaatatttta atagttatat cttagtctat tttccctaca atctcaacaa
     6001 agtattacat ttactgtcga aagaactttc ttccagaaaa aacaaaagcc gccgcatcag
     6061 acgagcaagg tatatgccgt ttgattctct acatagcgta aagtttaaca aaaacaaaag
     6121 ttaaccgaaa acatccgcct gaaaaattcg tgcgcgcaag ccccaataac tgctgattcc
     6181 cgtcgtataa tggaccattt tcccattttt gaccaaaacg acggcaggcg ttgcgacaat
     6241 ccgccaagac cttgccaaac ccccgtcctc atcgttgacc gtcggaaagc ccaagccgcg
     6301 ttttgccata tacgccgcca cttccgccga actgccggaa cgtaccgcca cgccgacgac
     6361 cggcacgccg tccgccgcca aatcatcgat tatcggcgac tgataacggc acacgccgca
     6421 ccagctcccc caaaaataca ccaaaaccgc cttatctcgg ctaaactgtc ccaaagtcag
     6481 ccgctgcccc gacagcaggg tcaaaggccg ccctgccgca ccggcaggtt cgtcgggctt
     6541 gcgtatccaa tccaaaaaca gcgacaccaa taaaaacacc aatgccgtct gaacggcaaa
     6601 tttgatgccc gaaagcagtt tctttttcat acgctctctc aaacggtacg cccgcgcaac
     6661 cggcaggcaa acaaaaagcc aagtctcaaa acttggcttc cggttatttg gtgggtcgtg
     6721 agcgattcga acgctcgacc aacggattaa aagtccgctg ctctaccggc tgagctaacg
     6781 acccgataag ccgtgcatta tacagcacca tcctgcctcg tcaagcaaat tttacaggct
     6841 taattgcaga ccactgtttg cacgggatat tttgacaacg gattttcaca atccgccgca
     6901 taccgtgtaa aagttcgcac aaggaaaggc aaaccgcccg aaatcaatgt acactttccg
     6961 cccgtttccc aaactgcaca cagaaacaca cattatgaac atacaaaaca tccgcaccct
     7021 cctcgacacc gtcgccgttc cgaatacggc acgcacgctt ggcggcgaaa aggccgtccg
     7081 ttcggtcgaa cagcgttcag acggcatcca tatcgccctg catttcggct tccccgtcgc
     7141 gcacattgcc tcagaaacag ccgaccgcat acaggaaatc ctgatgccgc tgacaggcga
     7201 cacacacatc catctgtcca tggacactga aatcggcaca cataaagtcc aacctggcgt
     7261 taccaccatc aaaggtgtga aaaacatcat tgccgtcgcc tccggcaaag gcggcgtggg
     7321 caaatcaaca accaccgcca accttgccgc cgcaatggcg cggatgggcg cgcgcgtcgg
     7381 cgtgctcgat gccgaccttt acggcccgag ccaaccgacc atgttgggtg tggacgaccg
     7441 caaacccgat cagaaaaacc aaaaactcat tcccgtcgaa tcttcagacg gcatacaggt
     7501 catgtctatc ggctttctcg tcgataccga ccaagccgtc gtctggcgcg ggccgatggt
     7561 cagccaagcc ttgcagcaac tgatgtttca aagcgagtgg gacgaagtgg attatctttt
     7621 catcgacctg cccccgggta cgggcgacat ccagctcaca ctgtcccaac gcatccccgt
     7681 taccgggtcc gtcatcgtaa ccacgccgca ggacatcgcc ctgatagacg cgcgcaaagc
     7741 cgtggatatg ttccgaaaag tcaacattcc cattttaggt gtattggaaa atatgtccgt
     7801 ccacatctgc tccaactgcg ggcatagcga agcactgttc ggcacggacg gcggcaaaga
     7861 tttggcggcc cgcctcaacg tccccctgct cggacagctt cccctaagcc tgcccgtgcg
     7921 cgaagccatg gacggcggca caccggcgca actgttcgac gaacaccccg ccatcgccaa
     7981 aatctacacc gatgccgcat tccaaatcgc cctgggcatt gccgacaaag gcaaagactt
     8041 cagcagccgg ttccctaaaa tcgtcgtcga ataaagccgc gtccgaaacc gcaacagcaa
     8101 tgccgtccca agccccgcgc ctgccgatat gccgtcattc ccgcgcaggc gggaatctag
     8161 aacgtggaat ctaagaaacc gtgttgtaaa ggcagaccga tgccgtcatt cccacgaaag
     8221 tgggaatcta gaacgtaaaa tctcaagaaa ccgttatacc cgataagttt ctgcaccgac
     8281 aggtctggat tcccgcctgc gcgggaatga cggttcgggt atttctgacg atttaggcat
     8341 tcccgacaag gtggattttc aaggtgttgt atagggtgta ggaggattcg taaaaggtga
     8401 gttatagggt gggcttcagc ccaccgattc caacgattcc accaatccta caccgttccc
     8461 atagactcaa atcaacacag aaacttatgc gccgtcattc ccgcgcaggc gggaatctag
     8521 accttagaac aacagcaata ttcaaagatt ataaaagacc cgtcattccc gcgtaggcgg
     8581 gaatctagac cattggacag cggcaatatt caaagattat ctgaaagtcc gagattctag
     8641 attcccactt tcgtgggaat gacgggatgt aggttcgcgg gaatgacggg atgtaggttc
     8701 gtgggaatga cgcggtgcag gtttccgtac ggatggat
//
LOCUS       DAWKQB010000084         8699 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.084, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000084 DAWKQB010000000
VERSION     DAWKQB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.084"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(252..3104)
                     /gene="gcvP"
                     /locus_tag="WJL14_001403"
     CDS             complement(252..3104)
                     /gene="gcvP"
                     /locus_tag="WJL14_001403"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ7656462.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVDEKSMDEFVGNTVPQ
                     SIRMPSELDLPEALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQNLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPKGVKRIADRIHALASAFADALVSDGLNVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISLQPNSGAQGE
                     YTGMLSIRRYQEAQGEAHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIIHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASAGQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIATLKSIRREVQKVIDGEWPKDDNPLVNAPHTAADITGNWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3135..3494)
                     /locus_tag="WJL14_001404"
     CDS             complement(3135..3494)
                     /locus_tag="WJL14_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656463.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLVGGFPLSLPLAVLLVLTCCILARCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3442..3633
                     /locus_tag="WJL14_001405"
                     /pseudo
     CDS             3442..3633
                     /locus_tag="WJL14_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
     gene            complement(3769..4410)
                     /locus_tag="WJL14_001406"
     CDS             complement(3769..4410)
                     /locus_tag="WJL14_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ7656464.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4400..4951)
                     /locus_tag="WJL14_001407"
     CDS             complement(4400..4951)
                     /locus_tag="WJL14_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ7656465.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLILKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4955..5473)
                     /locus_tag="WJL14_001408"
     CDS             complement(4955..5473)
                     /locus_tag="WJL14_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ7656466.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5473..7134)
                     /gene="pqiB"
                     /locus_tag="WJL14_001409"
     CDS             complement(5473..7134)
                     /gene="pqiB"
                     /locus_tag="WJL14_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ7656467.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7127..8500)
                     /locus_tag="WJL14_001410"
     CDS             complement(7127..8500)
                     /locus_tag="WJL14_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ7656468.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCRDEAL
                     PPHTVDCPECGCRADVPQLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
BASE COUNT         1935 a         2237 c         2457 g         2070 t
ORIGIN      
        1 tccactatat ttgtttgtcc gtgtggaaat gacatcaaag ggtgtgaata ggcttttcat
       61 ctttgaaatc tgtctgcccg atcggatgaa taaaaagtag gatgataaaa aagctgatgt
      121 atggatactg gtttaaccca tttgttttaa agttatagtc gagatacgtg tatcggtgca
      181 accagtctgc cgtcgacgaa aatgccgtct gaaagtgttt cagacggcat ttgtctccgg
      241 ggtttcaacg gtcagtcttc ataattttcc atcggtgggc agctgcacac gagattgcgg
      301 tcgccgtaca cgtcgtccac gcggttgacg aagggccaaa acttgtgttc gcggacgaac
      361 ggcaacggga agacggcttc ttcgcgggaa tacggatgcg cccagttgcc ggttatatct
      421 gcggcggtgt gcggagcatt caccagcgga ttatcgtctt tcggccattc gccgtcgatg
      481 actttctgca cttcgcggcg aatggatttc agggtggcga tgaagcggtc gagttcggct
      541 ttgctctcgc tttcggtcgg ttcgatcatc agcgtgccgg caacggggaa ggagacggtc
      601 ggggcgtgga agccgtagtc catcaggcgt ttggcgatgt cggtttcggt aatgccgctt
      661 tcggctttga gcggacgcaa gtcgacgata cattcgtgcg cgacgcggcc gtttttgcct
      721 gtatacagga tcggatagtc ttcgctcaga cgtttggcga cgtagttggc gttgagcaat
      781 gcccagcgtg tcgcctgttc catgccttgt ttgcccatca tggtcaggta catccaagta
      841 atcggcagga tggacgcaga accatatgct gcggcagcca cggcagtttg cccggcactt
      901 gcgctgtggg tgtcggtcaa agtgtgtccc ggtgcgaacg gagccagatg ggctttcaag
      961 ccaatcggac ccatgcccgg gccgccgccg ccgtgaggga tacagaaggt tttgtgcagg
     1021 ttcatgtgca acacgtccgc gccgacctcg gcaggctgca tgatgccgat ttgggcgttg
     1081 aggttggcgc cgtccatgta aacctgtccg ccgttctcgt gaataatgcg gcagatgtcg
     1141 cggatgcctt cttcgtacac gccgtgggtg gacggatagg taatcatgat ggcagacaaa
     1201 gcgtcgcggt gttgctcggc tttggctttc aaatcgtcaa tgttgacgtt accgtgttcg
     1261 tcggtgtcga caacgacgac tttcaagccc agcatggccg cagtcgcagg gttggtaccg
     1321 tgggctgatt ttggaatcag gcagatgttg cggtgcgctt cgccttgggc ttcttgataa
     1381 cggcggatag agagcatacc ggtatattcg ccttgtgcgc cggagttcgg ctgcagggaa
     1441 atcgcgtcaa agccggtgat ggctttcagg ctgttttcca tatcggcgag caattcgcgg
     1501 tagcccgcgg tttgcgcttc gggagcgtag ggatggatgt cggagaactc cgcccaagta
     1561 atcggcagca tttccgcagt cgcattgagc ttcatggtgc agctaccgag cgaaatcatg
     1621 ctgcggttca tcgccaaatc gcggtcttcg agttttttca ggtagcgcag catttcgtgt
     1681 tcggtgtggt aactgttgaa cacaggatgt tgcagaatat cgtcctgacg cagcaattct
     1741 gcattcagac ggcctttgac atcatcggca aatgcgaacg tatccttgcc ggtaaacgcg
     1801 cggtacaaat caaccaaatc ttcgcatgcc gatgtttcgt ggaatgcagc cgcaacttga
     1861 gtatcgttaa cgcggcgcag gttatagccc gattccaaag cagcggcaaa cacttggtct
     1921 gctttctctt tgttgccgaa atcgacggta acggtatcga agaagacctt atgaaccaca
     1981 ttcaggccgt ctgaaaccag cgcatcggca aaggcagaag ccagcgcgtg aatgcggtcg
     2041 gcgatgcgtt tcacgccttt ggggccgtgg taaacggcat acataccggc caagttcgcc
     2101 agcaatgcct gcgcggtaca aatattggat gtcgcttttt cgcggcggat gtgttgctcg
     2161 cgggtggaca acgccatgcg caaggcaggt ttgcccgatg cgtctttgga tacgccgatg
     2221 atgcggcctg gagcggaacg tttgaactcg tctttaaacg cgaaataagc agcgtgcgga
     2281 ccaccgaagc ccattggcac accgaagcgt tgagtgttgc ccaacgcaat gtccgcgccc
     2341 aattcagccg gagacttcag caaaaccaaa ctcatgatgt cggcggcaac ggccacgatc
     2401 gtgccttttg ctttcagacg gccgataacg tcctgcaagt tttgcacatc gccgtctttg
     2461 ccgacgtatt ggaacagcgc gccgaagtat tcgccttcgt cggcttgggc aaaatcgccg
     2521 accaccagtt caaagccgaa atatttggcg cgggttttca tcacgtccaa agtctgcgga
     2581 tacacgcgct cgtccacgaa gaaacgctcg gattttacct tgcccacgcg gtgcgccatc
     2641 gccatcgctt cggcggcggc agttgcctcg tccagcaaag acgcgcccgc cacgggaaaa
     2701 ccggtcaaat cgatgcacac ttgttggaag ttcaacaaag cttccaaacg accctgggca
     2761 atttccgcct gatacggcgt gtaggcggtg taccaacccg gattttccaa tacgttacgc
     2821 aaaatcacgt tcggcacgcg ggtcgggtaa tagcctaaac cgatatagga tttgttgatc
     2881 atgtttttcg acgcaatgcc tttcaatttt gccaacgcgt ccgcttcggt caaggcctcg
     2941 ggcaaatcga gttcggacgg catacggatg ctttgcggca cggtattgcc gacaaattcg
     3001 tccatgctct tctcgtcgac agcggcaagc aacgccgctt cgtcgccgaa actcaaatgg
     3061 cgcgcggcaa attcgttggg gttgaacagt tcggacagtt tcatttctac tccttcgggt
     3121 ttgtagggct gccatcaggc agaattttta gacatcggat gattcgggca agggtagcac
     3181 aaaagacggg ataatggcgg acaacgcgcc agaatgcagc aggtcaggac aagcagcacc
     3241 gcaagcggca gcgataaggg aaagccgccg accaaaacca tgaaacctgc aggcaccagc
     3301 caagctaagg gatgcgccgg aagcggaaaa acaatattga gcagaaccaa aatcacgaca
     3361 aacagcggca gcaccgccat caggttggca cacctgtatg ttgcgtacgg gtcggacgct
     3421 tcttcaggtt cgcaatattc catgatttca gcctgtccgg gctttatcca cagcaggctc
     3481 ggacggtcgg tcatgccgtc tgaaccggca ggccgtcttt ttcaaaaaat gccataccgc
     3541 tgcaacttca tgcggctgta tggcgtaggt tttgcacttc agacggcata tgcgtcccga
     3601 agcagtttgg tgtcccagtc ggttcatgat tatagtggat taaatttaaa ccagtacggc
     3661 gttgcctcgc cttgccgtac tatttgtact gtttgcggct tcgtcgcctt gtcctgattt
     3721 ttgttaatcc actatatata aacaacttgg gtgcggataa acctttgttt atctaaaccc
     3781 ctgttttctc aaaaattgat tcaaatcttc ggcaaatttc cgatagcctt tgccgttggc
     3841 gtggatttgg tcggatttca gattattatc gcccaaaatt tccgcccacg cgccgccgaa
     3901 cagcggaatg ccgtattcct cggacaaatc ctcatacagc ggatgatcgc tcaaatgccc
     3961 gaacaacgca cccagtgtga tgtgcggcac gccgacgagg acggcgggga tgttttcctt
     4021 ctgcacggtt tcgatgattt tcgcgatatt ggcgcgggtc tgctccttgg gaactttgcg
     4081 cagaaagtcg ttgccgccta tgccgacaat cacaagcttg ggtttgcgtg ccaacagcgc
     4141 gggcaggcgc gacagggctt gggcggatgt atcgcccgat acgccgccgt tgacaatatt
     4201 ccaacccgtc agtttttgca gttgcgcggg gtaggattcg ccggggtttg cgccgtagcc
     4261 gaaggtaagc gaatcgccca aggcaagtac ggtgcttcct tcgggaattt tggcgtgggt
     4321 tcgggcggat tttctgccgc aggcggtaag gagcaacgcg cctgcgccga ggaggaaggt
     4381 tcttctgttc attggttttt cagatggcat cgagccattt cgggtggtat ttcagggttt
     4441 cacggtaaac cggacagctt tcggcgtgcg cgcctttgag gtagccggta ctcatcagaa
     4501 attcgccgac gatttcgccg ccgacgaatt tgaaatgttt tttaaagagt ttaacccatt
     4561 cgtctttgct tcgcggatgg tgcgcgtcga gccagttctt gaacgagccg tattcttgtt
     4621 gcaacgcttg gatttgccgt gcattgaaaa tggcggcatc gattttcagg cggttgcgga
     4681 caatacccgc atcggcaagc agccgctcaa tatcggcttc gccaaatgcg gcaacagtat
     4741 cgatgtcgaa cccttcaaat gccgtctgaa acgcctgccg cttcttcagt atcagcgtcc
     4801 agctcaatcc tgcctgattg atttccaaca ccagccgctc aaacaattca ttgtcgtcct
     4861 caatcggaaa accgtattgg gtgtcgtggt aatgtttgtt cgggttgtcg gtgttttcgg
     4921 gaagtgaggc ggcaaattcg caatagttca cggtttactc gaccatctgt tgcgccgcct
     4981 gtttcagtcc ctgttcgagt gcggcggtca tggcggcgta gccgtcaccc tgctgttcgg
     5041 tttcgatatg gaagggtctg ttcgtaccgt cgggtaggac ggcgtagccg ctgatgaggg
     5101 ttttgcccgt gtagctgcct tggaatgcgt cgatatagac cgtccatttt tcggtactgc
     5161 cgctgcgtga ggcaggaaca aagatgcgtg tgctgtccaa acggttgaat gcattgctca
     5221 acgccgcttc gagcatatcg tccaaggtgt ctgcccagac gtggttttgt gcggtgttga
     5281 ggcggtaggg gtcggtttga tagaccagtc cgccgcgttt gagcggttcg gcaagacgga
     5341 cttcgacggc agtttcgccg ccttgcgttg caggacggat gtagcggctg tcgggcaaca
     5401 cgaaatattg tgtgctttgc acagtaccgc aggcggcaag cgtcagggcg gcggcaatcg
     5461 ggaagaggcg cattatcggc ttcctttcgg gatagggtct ttgctgctgc tgttgaaaat
     5521 cagcgcgttg ggtttttctt tcaaagtgtt aatgacgggt tgaacgtctt ttaaggtttt
     5581 gtccaaactt tgcagcgtat tttgtacgtc gccgtagata ggcgattgag gcgatacgcc
     5641 ttgcagggtt gtgcgcaact ctttcagggt ttggttcagt tcgttcggaa tgttttgtgt
     5701 ctgcggtttg ccgaccagtt tgtcgatgga gcttagggcg gcattggcag atttgagtgt
     5761 ggatttgagc tcggcaagcg aaccgttcaa ttcggcaacc gtcttatcta aaggcagttt
     5821 gtcgaacttg tccagcaaat ccgccaattt gacctgcaaa tcgtccaaac cgccgccctg
     5881 ggtcgcgata acggtatcgc ctgcataaac ggtatgcggt cgcagcttgg gcgaggcgga
     5941 aggctgatcg ttcaactcaa tcattttgct gccggtcagc aggttgttgc tggagatggt
     6001 ggcggtcagg cctttgttta aggccgtctg aaattgttgt ttccaatgtt ctttgctttg
     6061 ttcgtcggca ttgatttcca aacgggaagg ctcaatacgg atgcgtacgg gaatccagcc
     6121 gttttcaaac aggtgcaggc tgtcgttgcg gtcgaaataa ggaacatcgg aaaccacgcc
     6181 gacattcagc cctttgtact cgacaggcga accgacggtc agtccgcgca cggattgttt
     6241 gaaaaacgcg gtgtagtaca gcgaacggtc atcaggcagg ttggcgactt cgctgcggct
     6301 gtcgtaaagc gtgaagctgt cttcgctttt gacgttttta ctgtttttgg ttttcggcga
     6361 atcaaatgaa atcgcgcccg acagcagggc aggcagaggg gcggaattga gtttgatgcc
     6421 gctgcctgtg gtttcgatat tgatgccgct ttccagccag aaacggctgg cggaatgaat
     6481 cagtttgtcg ttggggcttt ggatgaagat ggtgtaatgc acgctttggt cggacgggtc
     6541 gaaatgcgcg ctttcgactt gcccgaccat aaagttttca tacaaaacag ggctgttgac
     6601 gttgaggatg cggtcgtttt taccaatcaa attcaagcgc agcccgcttt gcccgatggc
     6661 ggtaacgggc ggaatgtcct gcacttggaa cacgtctttt gcctcgtcgc ttttgccggg
     6721 tgtaaaggcg atgtacgaac ccgaaagcag cgtacccaaa ccggttacgc cgctttggtc
     6781 gatacgcggc ttgaccaccc aaaactgggt atcgctgcgg atgaggccgg atacgtccgc
     6841 attgagttgg gcggttactt ccacgccttt ttggtcgtcg cgcagtttga ttcgggtaac
     6901 gcgtccgaca tcgatgctca atactttgat gaccgtattg ttgacctcaa tgccttccgc
     6961 gctgtccatc aagagcgtaa ccacaggccc cctgttgcgg atttccttaa cccaaagcca
     7021 gccgccggca atcagcgcga tcagcggaac cagccagacg gcagagagga aggtgttgtt
     7081 tttgcggacg cgtgcttggg cgtgtccgtt tggaggaggg ctgttgtcag tcatgttttt
     7141 ccgtttcatt gaaagcaatg ccgtctgaag cgcgtttgtc ccaaagcagg cgcgggtcga
     7201 aataataggc ggacagcatc gtcagaatca cgaccaggca gaaatagact gccgcactgc
     7261 ccggaatgac gcgcgcggca taagtgtgga acgaacacat caaaataata atcacaaaaa
     7321 tatcaatcat cgaccagcgg ccgaccgctt cggtgatgcg gtagaggtgc gacaatttct
     7381 ttgcacccgt tggcaaagcg aagcgggcgg acgcaatcaa aaccgacatt gccgcaatct
     7441 tcagtaccgg caccaaaata ctcgcgctga aaataaccgc cgcaatcagc ctgtcgccct
     7501 cgtcccacat ataagcgatg ccgttaagga tggtattgac ctccgtggcg gcaggattgg
     7561 acgaaatcat aatcggcagg atattggcag ggaaatacaa aataaccgcc gccgtcagaa
     7621 acgccgacga aatactcaga cttttcggcc gtcggcggta cagttccgca ccgcacacgc
     7681 cgcaggggga ttcggcactg tcgcggaaat acaggcagcg gctgcaacag gttttacctt
     7741 ccgatgccgt ctgaaccgca ttatcccccg tcagccgccc gatttgaaaa tacacccaat
     7801 gctggggaac cgataccgaa gtccgaatca gcataaccga cagcgcgaac atcagataaa
     7861 acgccgatcc gaagcgaacc tctgccacag acgagagctt gatatacgcc accaaagtgg
     7921 aaacaaaaaa cacatccacc atcatcgcct gtctcaagcg caccatcaca cgcgttgcca
     7981 aacgcagcgc aggatacgcc tgtttccgta tcagcgcggc atagacatac aggcacagca
     8041 gcagaaacag aaccggcgcg ccgaaggtca gcacaaacat cacttcggcc aaaaaaccat
     8101 aatcctgaaa caccatcagg cgcatcatct cgggcagcga aaggacggat gccgcacccg
     8161 gtatcccgac ctcgatatac gtcataccgt aagcaaacgc catcaaaatc agcgaagccg
     8221 ccgcataagc gggcggggcg gaaaaaggat gcctgcccac cctgaagagc ttatagccgc
     8281 agcgcgggca gaacgccgcc tcccccctgt ccaattgcgg cacatccgcg cggcagccgc
     8341 attccgggca atctaccgta tgcggcggca gggcttcgtc gcggcagttg ccgcgaaggc
     8401 tgtgggggat attttcggca aacggcttca tcgggaaagt ggcaaatata agtggcggca
     8461 ttataaagga aaaaaggcga tacacccaat caggatgcaa aaccggcagg ttcggcagta
     8521 tgaacaagcc cttggtgtcc ggcaaaaata tttttcgagc ggctgtccgc cgactgccga
     8581 acccatttga taaaatcccg attctgtaaa gaatagtaaa atacctgctt aaaaacatca
     8641 cgcaaatgat attttttcca aaaatatgtg ataataaatt tcgtttatga ttctttatt
//
LOCUS       DAWKQB010000085         8661 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.085, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000085 DAWKQB010000000
VERSION     DAWKQB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.085"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(171..863)
                     /locus_tag="WJL14_001411"
     CDS             complement(171..863)
                     /locus_tag="WJL14_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ7656469.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKMPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERARKYLLPRLEKHAAETGLTPAATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="WJL14_001412"
     CDS             complement(971..2164)
                     /locus_tag="WJL14_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7656470.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJL14_001413"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="WJL14_001413"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ7656471.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="WJL14_001414"
     CDS             complement(3017..3946)
                     /locus_tag="WJL14_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ7656472.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKL
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQVYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJL14_001415"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="WJL14_001415"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ7656473.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="WJL14_001416"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="WJL14_001416"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ7656474.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQSGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDSGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQLSLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKPAYDSYGNTRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7216..7755
                     /locus_tag="WJL14_001417"
     CDS             7216..7755
                     /locus_tag="WJL14_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ7656475.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(7945..8160)
                     /locus_tag="WJL14_001418"
     CDS             complement(7945..8160)
                     /locus_tag="WJL14_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656476.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8168..8371)
                     /locus_tag="WJL14_001419"
     CDS             complement(8168..8371)
                     /locus_tag="WJL14_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656477.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8284..8643)
                     /locus_tag="WJL14_001420"
     CDS             complement(8284..8643)
                     /locus_tag="WJL14_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ7656478.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
BASE COUNT         2152 a         2400 c         2154 g         1955 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccatgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcgacgtat tcgggcgtgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcagcgggag tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtatttg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcattttag caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa gccgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aataggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatacc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtta gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgattag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgtcca ctgctttgac ctgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtccacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagataga
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgcgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga caatcacctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggtg cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cagtttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattgccc gccgccaaag
     3841 caaccgtgtc cattgcaccg gcatgaccga tgccgtaacg ccgatgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtaaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggcgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt actgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc cttatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactc tccgccgtaa cctggccgcc gcaatccgcc
     4861 cttgtgctgg caggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg catcgcatgg
     4921 ctgttgcaaa gcggacaagc cagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgtttgggc gcgatgattc ccatcaatgt ccgcgccatg
     5041 tggctcggca cgttccacgg tctctgccac cgctttttgc gcctgcacca ccgcgacgca
     5101 ggcctgccgt cttcctttca aatcctcgac agcggcgacc agctttccct gatcaaacgc
     5161 ttgctcaaaa gcctcaacat cgccgaagaa atcatcgcac cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggcttgcgc gcttccgtgt tgagcgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggaa acgcagcggt atttgccgtc
     5521 ggcgacgacg accaaagcat ttaccgtttc cgtggcgcaa acgttggcaa tatgaccgcg
     5581 ctgatggaag aattccacat cgacgcgccc gtcaaactcg aacaaaacta ccgctccgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtt atcgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaattcgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cgtggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctatcgcagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ttacaaaatc tacggcggcc tgcgctttta tgaacgccaa
     5941 gaaatcaaac acgccctcgc ctacctgcgc ctcgccgtca atcccgacga cgacaacgcc
     6001 ctcttgcgtg tcatcaactt cccaccgcgc ggcatcggtg cacgtaccgt cgaaaatctt
     6061 cagacggcct caaacgaaca aggcatcacc ctctggcaag ccgcctgcaa cgccggcgcg
     6121 aaagccgcca aagtcgtcgc cttcgtccgc ctgattgaag ccctgcgcaa ccaagtcgga
     6181 caactgtccc tgtccgaaat catcgtcggc atcctcaaag acagtggctt gaccgaacac
     6241 taccgcaccc aaaaaggcga caaccaagac cgtctcgaca accttgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aacttcgaaa tcctgcctga aaacatttca
     6361 gacgatcccg ccttccccat cctcgctttc ttgagcaacg ccaccctcga atccggcgaa
     6421 aatcaagcag gtgcaggcga aaaagccgtc caactgatga ccgtccacgc cgccaaaggc
     6481 ttggaattca acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agcctcgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agcctgccta cgacagttac ggcaacacgc gccaaaccgc cgcatccaaa
     6781 gataaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt cggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa tttcggcaaa cagggcgtga aagagttgga caccaagttt
     6961 gcgaaattgg aagagatgta gaagggagat ggcagaggcc gtctgaatcc ggtaacaaca
     7021 aattttgccg aaacctccgc cgtcattccc gcgcaggcgg gaatccagac ctttcggcac
     7081 ggaaacttat cggataaaag gtttctttag attccacgtt ctagattccc gcctgcgcgg
     7141 gaatgacgga cggtaaaagc cgggtataga tacctaaccc ttgtttatag ctgaaaatca
     7201 ataaggaaga acattatgtc ccaagttttt aaagattttg atttgtcctc cgtatgggaa
     7261 tccgacagtt gggcagatga aaactaccaa gaagccccgt ttacccctga aattttggct
     7321 gccgtagaaa gtgaactggg ctataaattg ccgcaaagtt ttattgaatt gatggcggta
     7381 caaaacggcg gaatatttgt caaaaactgt tttccgacca cacagagaaa ttcgtgggcg
     7441 gaaaatcatg tgcaaatttg cgaggtatcg ggcatcggct ttgaaaaaga agggagtttg
     7501 tgcggcgaaa cagggcaaaa actttggctg gaagaatggg aatatccgcc catcggcgtg
     7561 tattttgcca acgacccgtc aggcggtcat gccatgtttg ccttggacta tcgggcgtgc
     7621 ggcaaagacg gcgagccgaa agtggtgttt gtcgagcaag aatcggattt tgaaatcgtc
     7681 gaacttgccc ccgattttga aacctttatc cgcagcttgc ggcatgaaga tgagtttatt
     7741 gacgaggaaa tataaaacgg cggttgaaaa accggaatca tcaagggaaa acgggcgaaa
     7801 taacggataa tcgcttgaat ccgtaaggaa aacggtttgg tggaacgcgc catccaagac
     7861 ctttgcaaag aaaaactgtc tccgacagca ttgacattat taacagaact tatcaagttt
     7921 ggagctatct caaatataat tcggttatcc ttttgtatcc attaaatcat atgcttcaat
     7981 taattgttgt tctagctctt gtaccaattt tgaattgcga attcctgaca caatctcaaa
     8041 ttcttctgca tctatgcaaa cacctgcata aatttcaata acaagggaac gcaataattg
     8101 aagctcttct cttgttaaag aaataataat gtcatcacct gtgtaattga ttatattcat
     8161 aataatttta tttttgtttg tcaaagtaag ttttgcctaa ggttggtcta aatgcagttc
     8221 caccatcttt tgaatttggg tctctgatta caattgctcc agacttatca tcccaaattg
     8281 ctcttatgtg tttggattgt aatcttcgaa ttcccaagaa aaaaatcgta ataagtttga
     8341 aagtgtcaaa tcccaagttt cttttgagca atattctaat attttatcaa tttcactttt
     8401 aataatctta tgatcaaact gttctaatat taatgcatta gaccaaaaaa aaccttcttt
     8461 attacaatga tgggaaatcc atttaggaga acaaatgcaa agtgaaaaaa tagatgagcc
     8521 ttgttctcct tcgattccga tatccaaatc tatccatcta tggaaattat ctggaatttc
     8581 gggggtaaat ttttcaaaat caatatcata taaatttatg ctttttaaat ccaatttaat
     8641 cattagggct gtcctagata a
//
LOCUS       DAWKQB010000086         8617 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.086, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000086 DAWKQB010000000
VERSION     DAWKQB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.086"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..284)
                     /locus_tag="WJL14_001421"
     CDS             complement(<1..284)
                     /locus_tag="WJL14_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7656479.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTV"
     gene            complement(554..1657)
                     /locus_tag="WJL14_001422"
     CDS             complement(554..1657)
                     /locus_tag="WJL14_001422"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ7656480.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1737..3293)
                     /gene="dnaA"
                     /locus_tag="WJL14_001423"
     CDS             complement(1737..3293)
                     /gene="dnaA"
                     /locus_tag="WJL14_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ7656481.1"
                     /translation="MTLAEFWPLCLRRLHDMLPHGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVCYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMRNLPHEPRQAAGSASRPESAA
                     VAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3581..3715
                     /gene="rpmH"
                     /locus_tag="WJL14_001424"
     CDS             3581..3715
                     /gene="rpmH"
                     /locus_tag="WJL14_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ7656482.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3718..4083
                     /locus_tag="WJL14_001425"
     CDS             3718..4083
                     /locus_tag="WJL14_001425"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ7656483.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            4149..4370
                     /gene="yidD"
                     /locus_tag="WJL14_001426"
     CDS             4149..4370
                     /gene="yidD"
                     /locus_tag="WJL14_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ7656484.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4542..6179
                     /gene="yidC"
                     /locus_tag="WJL14_001427"
     CDS             4542..6179
                     /gene="yidC"
                     /locus_tag="WJL14_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ7656485.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQTAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKSEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(6260..6973)
                     /locus_tag="WJL14_001428"
     CDS             complement(6260..6973)
                     /locus_tag="WJL14_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ7656486.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(7032..7622)
                     /gene="maf"
                     /locus_tag="WJL14_001429"
     CDS             complement(7032..7622)
                     /gene="maf"
                     /locus_tag="WJL14_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ7656487.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVDVL"
     gene            7770..8273
                     /locus_tag="WJL14_001430"
     CDS             7770..8273
                     /locus_tag="WJL14_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ7656488.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8307..8486
                     /gene="rpmF"
                     /locus_tag="WJL14_001431"
     CDS             8307..8486
                     /gene="rpmF"
                     /locus_tag="WJL14_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ7656489.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKVKGE"
BASE COUNT         2107 a         2562 c         2062 g         1886 t
ORIGIN      
        1 acggtactca acgcctcaat gcccgtagct gctttaccgg ccgccgagtt ggtatggaac
       61 tcaatcgcca catccgagcc gcgaatcagt tttacagctt cacgcagcgg catattgcct
      121 ttgcctgtgc cgtcggtttt aacagtcaaa ccgtagtcat cgcgcaaaat agcagccacg
      181 atgttgcgca tatcctgcgc caagtccgcc tcacggtcgc tgccgttgac cgcacccggg
      241 tcggtgttgc tgtgtccagc ggttaaggtt acggttttgc ccattcatca tctccaaaaa
      301 atattgattg catatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag
      361 tacagatagt acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt
      421 gagctaaggc gaggcaacgc cgtactggtt tttgttaatc cactatacct ttccgacagc
      481 cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac gggcatcgtg
      541 ctccggaaaa ccgtcaaatt cgcatcggca tcacgatata tttgaaattc ggattgttcg
      601 gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata tcgtcggaat
      661 ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg agttcgccgc
      721 cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg ctgcacacga
      781 cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg ttggcaagaa
      841 tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa atcttgtcgt
      901 tgtccaaagg aatcacgcgg ttgaaatcgg ggaatttgcc gtcgatgacc ttgctgacga
      961 tggtcgtgcc gttgcattgg aaacgcacct gtttgtccag cagctcgatt tgaatcggat
     1021 cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc aaaatcactt
     1081 cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg tgtccgtcgg
     1141 tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg agataatagc
     1201 ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg aaacgctcct
     1261 gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga aaatcggcgg
     1321 caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga cggctgtcgt
     1381 cccaatccag cgacaccagc gcaccggcag gcagtgcccg taaaatgtcc tgaaatttct
     1441 tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca gtgtcgattt
     1501 ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca atcaggacgt
     1561 tggacaggat gggcagggta tggcggcgtt cgacgatgcc ggtaacggct tgcagcggct
     1621 tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatttt cgctccttaa atcggttgga
     1681 ttttttaccc ttcggacggc atgggcaata tcatgccgtc tgaaaggcgc gttcggtcag
     1741 ttttgaatca gaatcagcag tttctcgtaa tcctgcgcca actcggggtc ttcctcgcgc
     1801 agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgtcc gccaaacgaa
     1861 tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat ggcaacctga
     1921 cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat tttgatgcgg
     1981 taatatttcg ccaccgcatc gatgatgata tcggcggtga tgactttgtg cttctcggca
     2041 ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg gttcataaag
     2101 cggctgctcg ctccgacacg attaaacgcg ccttcaagct cgcgcacgtt ggaacggatc
     2161 agattggcaa tgaacagcgc ggcttcgtct tcgatactga tgcccgccgc ttccgccttt
     2221 ttctgcaaaa tggcgatgcg catttccaat tcgggcggct cgagttccaa agtcagtccc
     2281 catgaaaaac gggatttgag gcggtcgtcc atgccttcga ttttcgcagg caacacatcg
     2341 caagtgagga tgagctgttt tttctcgttg tggaaatggt tgtacagata gaaaaactct
     2401 tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag caggtcgtat
     2461 tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt cataaagctg
     2521 cggatgtagt cgtccgaatg catatagcgc actttggcat cgggacggtt tttcaacagt
     2581 tcgttgccga ccgcctgcac aaggtgggtt ttacccaaac ccgtactgcc atagaggaag
     2641 aacgggttgt aactctgtcc cgggctttcc gcaatcgcct gcgccgcagc cgccgcaagg
     2701 cggtttccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag attggtctgc
     2761 tcgtaacgcg cctcttccgc atcgcgctgc gcgtccgtcc gtgctttggc aactgccgcc
     2821 gattccggcc gggaagcaga cccggcagcc tgacgcggct cgtgcggcag gtttctcata
     2881 cgttctgcca aaatatccgc cgccgttttc gacgcagcgg gtttgacagg ctcttcagac
     2941 ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc gtgcaaggac
     3001 ggctcggcag gttcgacagc accttcaacc gccgccatct cataacacac gccttctccc
     3061 ggtttgaata cgaaggcgga acggccggca gccaactctt ccctcaccgc ttctattttt
     3121 ccggcaaact ggctcttgag catattgcag gcaaactggt tcttgccgta caccacccat
     3181 acgcccccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg cccgtgaggc
     3241 aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt catggatagg
     3301 ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca aaaagaaaac
     3361 aaatctgttg aacgcgcgtc ggttcaaaac gcgactgccc gattataccg actcacgaat
     3421 attttatcca caacccgtgc aaaaatttat ccacagaaag gcggcagaaa tccgcaggca
     3481 atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa attggagtgt
     3541 aattcacggt ttatctatct accgattttt aggaaacatc atgaaacgca cttatcaacc
     3601 ttccgttacc aaacgcaaac gcacccacgg cttcctcgtg cgctccaaaa cgcgcggcgg
     3661 acgcgcagta ttggccgcac ggcgcgccaa aggccgcaaa cgcctggcgg tgtaattttg
     3721 gactaccgct tcggaaggca gtaccgcttg ttgaaaacgg atgatttttc atccgttttt
     3781 gcattcagaa accgccgcag ccgcgacctg ctgcaagttt cgcgttcaaa cggcaacggg
     3841 ctggatcatc cccgcatcgg tctggtggtc ggcaaaaaaa ccgccaaacg cgccaacgaa
     3901 cgaaattata tgaagcgcgt catccgcgac tggttcagat tgaacaaaaa ccggctgccg
     3961 ccgcaggatt tcgtcgtgcg cgtccgccgt aaattcgaca gggctaccgc aaaacaggca
     4021 agggcggaac tggcacaact catgttcggc aaccccgcaa ccggatgcag gaaacaggca
     4081 tgatcagaac ggtactctgc aggcaaggtt cagacggcaa cgggtttccc atactaagga
     4141 acatcccgat gaacttccta ttgtccaaac tcctgctggg actgatacgg ttctaccaat
     4201 attgcatcag cccgctgatt ccgccgcgct gccgttatac gccgacctgt tcgcaatacg
     4261 cggtcgaagc ggtcaaaaaa tacggcgcat tcaaaggcgg ccggctcgcc atcaagcgca
     4321 ttgcacgctg ccaccctttc ggcggacacg gacacgaccc cgttccctga cccgacgcaa
     4381 tattcaaatt gcacgctttc cttttatttc ccatcggttt ctatataatg ccgtctgaac
     4441 tttcgggctg gcggcacgac cgtcgggtat gaagcccgcc cttattcccc gtctatcgga
     4501 acacgcaacc tgcggcattt ccgaccattc aggaaactct tatggatttt aaaagactca
     4561 cggcgttttt cgccatcgca ctggtgatta tgatcggatg ggaaaagatg ttccccactc
     4621 cgaagcccgt ccccgcgccc caacagacgg cacaacaaca ggccgtaacc gcttccgccg
     4681 aagccgcgct cgcgcccgca acgccgatta ccgtaacgac cgacacggtt caagccgtca
     4741 ttgatgaaaa aagcggcgac ctgcgccggc tgaccctgct caaatacaaa gcaaccggcg
     4801 acgaaaataa accgttcatc ctgtttggcg acggcaaaga atacacctac gtcgcccaat
     4861 ccgaactttt ggacgcgcag ggcaacaaca ttctaaaagg catcggcttt agcgcaccga
     4921 aaaaacagta cagcttggaa ggcgacaaag ttgaagtccg cctgagcgca cctgaaacac
     4981 gcggtctgaa aatcgacaaa gtttatactt tcaccaaagg cagctatctg gtcaacgtcc
     5041 gcttcgacat cgccaacggc agcggtcaaa ccgccaacct gagcgcggac taccgcatcg
     5101 tccgcgacca cagcgaaccc gagggtcaag gctactttac ccactcttac gtcggccctg
     5161 ttgtttatac ccctgaaggc aacttccaaa aagtcagctt ctccgacttg gacgacgatg
     5221 ccaaatccgg caaatccgag gccgaataca tccgcaaaac cccgaccggc tggctcggca
     5281 tgattgaaca ccacttcatg tccacctgga tcctccaacc caaaggcgga caaagcgttt
     5341 gcgccgctgg cgactgccgt atcgacatca aacgccgcaa cgacaagctg tacagcacca
     5401 gcgtcagcgt gcctttagcc gctatccaaa acggtgcgaa atccgaagcc tccatcaacc
     5461 tctacgccgg cccacagacc acatccgtta tcgcaaacat cgccgacaac ctgcaactgg
     5521 ccaaagacta cggcaaagta cactggttcg cctcccccct cttttggctt ttgaaccaac
     5581 tgcacaacat catcggcaac tggggctggg cgattatcgt tttaaccatc atcgtcaaag
     5641 ccgtactgta tccattgacc aacgcctctt accgttcgat ggcgaaaatg cgtgccgccg
     5701 cgcccaaact gcaagccatc aaagagaaat acggcgacga ccgtatggcg cagcaacaag
     5761 ccatgatgca gctttacaca gacgagaaaa tcaacccgct gggcggctgc ctgcctatgc
     5821 tgttgcaaat ccccgtcttc atcggattgt attgggcatt gttcgcctcc gtagaattgc
     5881 gccaggcacc ttggctgggt tggattaccg acctcagccg cgccgacccc tactacatcc
     5941 tgcccatcat tatggcggca acgatgttcg cccaaaccta tctgaacccg ccgccgaccg
     6001 acccgatgca ggcgaaaatg atgaaaatca tgcctttggt tttctccgtc atgttcttct
     6061 tcttccctgc cggtctggta ttgtactggg tggtcaacaa cctcctgacc atcgcccagc
     6121 aatggcacat caaccgcagc atcgaaaaac aacgcgccca aggcgaagtc gtttcctaaa
     6181 tgccgcagca tgaaaaatgc cgtctgaaac ctgttcagac ggcattttta ttgttcaccc
     6241 cctatcgggg cggaaatctt caacctgcat acatcacaaa aatcgtcggg cgttttttca
     6301 gattgggcat ttcttttctt tttcgccact gcacaaccgt ctggctgatg atttcctgag
     6361 tcggcaacgt caaatccgta gccgtacaca aacgcgtttc agaatgcagg ttttccaccg
     6421 catccgccaa caacacatcg ttgcgatacg gcgtttcgat aaacagctgc gtttcattct
     6481 gctggcgcga acgttgctcc aaagccttca aactctgaat ccgctcgttt ttttcagacg
     6541 gcagataacc cttaaacgca aaactctgcc cgttcgcacc cgaagccatc aaagccagca
     6601 gcaggctgga aggcccgacc agcggacgca cttcaaaacc gtgtttatgc gccaaagcca
     6661 ccagattcgc acccggatcg gcaacagccg ggcaacccgc ctcgctgaca atgcccatac
     6721 tgcgcccttc ttgcaaaggt ttcagcaatt ccagcaaagt cttcaaatcc gtgtgttcgt
     6781 tcagcgtttg caaattcagt tcgcggatag gcattgtcac gcccaaatgt ttcaaatgcg
     6841 cgcgcgccgt tttttccgct tccacgacaa aatccgtcag cccgacaatc gcctgttgtt
     6901 catgcggcaa caggcacggc gtatcgggcg tacccaaagg cgtaggaatc aaatacaaaa
     6961 caggagacat cattccctca ctcatcggtt aaaaatgccg tctgaaccct tcagacggca
     7021 taaacgggca gttacaaaac atccacgccc tcatttttca aaaaatcgac cagacggaaa
     7081 accggcaaac cgattaaagc attcggatcg gtactctcaa tccgttcgat caacaatgca
     7141 cccaaagcct cgctcttcaa cgcacacgag caataaaccg catcaggctc gcgctccaaa
     7201 tagcggagga tgtgcagctc gtccaacttc ctcatcacga ccaccgtctt atcgatatgc
     7261 cgctgcatcc tgcccgtaac cgtatttagc agaacgaccg cgctgtaaaa ctcaatctcc
     7321 ctgccgctca aatgcatcag catcttttgc gcgttggcaa ggttcatcgg cttgccccac
     7381 tgcctgccgt cgcaccacgc cacctggtcc gcaccgacaa tcaacgcctc ggggaaacgt
     7441 ccggccaacg acctcgcctt accctcggca aggcgcaatg ccgtctgagg ggcggattcc
     7501 cccaacatcg gcgtttcgtc aaaatcgggg gacgccgcct gaaaggcaat gccgagcctt
     7561 tccatctgtt cgcggcggaa aaccgaactc gtacccaaaa tcaaaggcag ttccaaaccc
     7621 atcccatcct ccttaccgtt gaaaacacgc ccgaaggggc agtaaaatcc agccatgcgc
     7681 cgaaacacgg atacccgcct tcggcgtacc gcaacatttt tcttaaaaat attgacgtta
     7741 gaacatctaa attatatcat atcccgttta tgtcagaccc taatttgatt gacccggaaa
     7801 tttttgccgc cgaagggcag aacctgcaag gcagttttct gctggaagaa ttggatgaac
     7861 gcgtcagttc gcacgattat cccgccgaca ggcagaccaa aatatcgttt acactgaccg
     7921 gcggtcgcga ccggctgcaa cgcctgttcc tcgacctgaa cgtcaaagcc gatatgcccc
     7981 tgatttgcca gagatgtatc aaacccatgc cgttcatgct tgatgaaagc agccgtatcg
     8041 tcctgttttc caacgaagag tccttggacg aatccatgct tgccgacgaa gaactcgaag
     8101 gcatactgat tgaaaaagaa ctcgacgtgc gcacattggt agaagaccaa atcctgatgt
     8161 ccctgccctt ttcgccgcga cacgaagact gcggcgacaa tgggacactg gaagaagtca
     8221 atcgggacaa acccaacccc tttgctgttt tggcaggttt gaaaagcaat tgattaggac
     8281 acagtttatt tatctaggag cttgaaatgg ccgttcaaca aaacaaaaaa tccccttcca
     8341 aacgcggtat gcaccgttcg cacgacgcgc tgaccgcgcc tgcactgtct gtcgacagca
     8401 caaccggcga agtacaccgc ccgcaccaca tctcccccaa cggtatgtac cgcggccgca
     8461 aagtggtcaa agtcaaaggc gaataatccc tattcgactg actgaaaaag ccagaacatt
     8521 gccatgcaat tactggcttt ttttgcattg gacgcaccat ccgtccaaac tttcgccata
     8581 cgtcaacaca caggggcaaa gcgttccgta taatacc
//
LOCUS       DAWKQB010000087         8546 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.087, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000087 DAWKQB010000000
VERSION     DAWKQB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.087"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(80..598)
                     /locus_tag="WJL14_001432"
     CDS             complement(80..598)
                     /locus_tag="WJL14_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ7656490.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(618..2003)
                     /locus_tag="WJL14_001433"
     CDS             complement(618..2003)
                     /locus_tag="WJL14_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ7656491.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            2242..3783
                     /locus_tag="WJL14_001434"
     CDS             2242..3783
                     /locus_tag="WJL14_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ7656492.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAGASALVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGGYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETRQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPGWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITLPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFAVCIFLLLDGGEGGK
                     RTETL"
     gene            3812..4411
                     /locus_tag="WJL14_001435"
     CDS             3812..4411
                     /locus_tag="WJL14_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ7656493.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            4408..5013
                     /gene="nadD"
                     /locus_tag="WJL14_001436"
     CDS             4408..5013
                     /gene="nadD"
                     /locus_tag="WJL14_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ7656494.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRHGETYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHKMPGELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRHNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            5072..5458
                     /gene="rsfS"
                     /locus_tag="WJL14_001437"
     CDS             5072..5458
                     /gene="rsfS"
                     /locus_tag="WJL14_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ7656495.1"
                     /translation="MNEQELQDLQKMVEVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            5512..5982
                     /gene="rlmH"
                     /locus_tag="WJL14_001438"
     CDS             5512..5982
                     /gene="rlmH"
                     /locus_tag="WJL14_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ7656496.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWQQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            6057..6323
                     /locus_tag="WJL14_001439"
     CDS             6057..6323
                     /locus_tag="WJL14_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ7656497.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWMAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            6480..7163
                     /locus_tag="WJL14_001440"
     CDS             6480..7163
                     /locus_tag="WJL14_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ7656498.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            7287..7799
                     /gene="coaD"
                     /locus_tag="WJL14_001441"
     CDS             7287..7799
                     /gene="coaD"
                     /locus_tag="WJL14_001441"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ7656499.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            7831..8487
                     /locus_tag="WJL14_001442"
     CDS             7831..8487
                     /locus_tag="WJL14_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ7656500.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFDYQNRQIEIVAPLKNEWQTENIHRALEEFCMENKID
                     "
BASE COUNT         1861 a         2262 c         2505 g         1918 t
ORIGIN      
        1 atcaatgtaa tccattctgt tcccgatgtt tatgccgtct gaaacccatc ctgtgtcggg
       61 cttcagacgg catattgctt caaagcaggt tttccgaggc aacccagttc agaatatcgg
      121 cttcgaccgc ttcgggcgtg ccggggttgg cgattttgac cccgtattcg ccttcgccca
      181 gttcctcgag gatttccagt tgcgaatcga gcatccctgc tttcatgtaa tgtcctttgc
      241 gcgacatcat gcgctcgagg ttgatgtctt gcggcggact gaggtggatg aaggcagctt
      301 tgccttcggc tccgcgcaga atgtcgcggt agccgcgttt gagggcggaa caggttacga
      361 tggtgtggtt cgcaccgttt tgcgcctgtt gcgtcatcca gtcgcgcaga ttgcccaacc
      421 acggatagcg gtcttcatcg gtcagcggaa tacccgcgcc catcttgtcg cggttggctt
      481 gggtgtggaa ctcgtcgcct tcggcatagg gacattgacc gaggtgtttc tgcagggaca
      541 gcgcggcggt ggtcttgccg cagccgcata cgcccataac gacaaaatgc gtagtcattt
      601 actatccttt ccgtctgtca gacgatggcg aacagcagtg cggacaaggc aaagccgatg
      661 agtgcgatga gggtttggtt gaccgtccag gttttcagcg tggtcggtac gtccatgtcc
      721 aagagacggc cgaccagcca gaagccggag tcgttgaagt ggctgcaacc gaccgaacct
      781 gccgccgttg ccaatacgat acaggcgagc tgccagtcgg taaagccggc ggcggcaacg
      841 gcaggagcca tcagcgcggc ggcggtggtc agggcgacgg ttgccgaacc ttgcgcgata
      901 cgcagtgcca aggcgacaag gaaacagccc aaaaggacgg gaatgcccaa atccgccatg
      961 ctgtcggcga gtgccttgcc gatgccggaa gcgcgcaaaa cgccgccgaa cataccgccc
     1021 gcgccggtaa tcagaatcac ggaacagacg ggggcgagtg cgccgtccac ggttttttcc
     1081 aacgcgctgc cgctttcgcc gcgtttgcgt cccaagacaa acagtgcgac caatacggaa
     1141 atcagaaggg cgatcggtgt cgaaccgatt atttttgccg tctgaaccca ggtttcgtcc
     1201 gcacttacga gtttttcgct gatgagggcc gatacgccgg tattcaggaa aatcagcagc
     1261 atgggaatca gcatgatggc gacgaccgtt cctgctttgg caggttcttt cggcaggtcg
     1321 ttgtcttgcg tgccgccgct gagcagttcg ggaacgggaa catggatggt gcgccccaac
     1381 actttgccga gcatatagcc gctgaaatac catgtgatga aggcggtcgg cagacccaaa
     1441 atcaaaactt ggccgatgtt cgcgccgtaa aattcggaag cggcaatcgg gcccggatgg
     1501 ggcggcagga agacgtgcat gacggaaaat gcgccgatgg aggcaagcgc gaagggcagt
     1561 acgtcctgtt tcatgcgccg tgcggtggcg aacacgatgg gcagcatgac gattagtccg
     1621 gcatcgaaga aaatcgggaa gccgaaaatc agcgaggcaa cgcccagcgc gaacggtgcg
     1681 cgtttttcgc cgaacatccg gatcagcgcg tccgccagcg actgtgcgcc gccggatgtt
     1741 tcgaccagac gtccgagcat cgcgcccagg ccgaccagaa gcgccacgcc gccgagcgtg
     1801 ccgccgaagt ttttgaccag tacgtcgttg acaatgctgc ctgtgggcaa accggttgcc
     1861 aaagccgtca gcaggctgac gatgaccagt gtcagcagcg cgtggatgcg gaatttgacg
     1921 attaaaatca gaatgaggat gattgccgcc gccgaaatgc ccaacagggt ttgcgcggac
     1981 agcgtctgtg tccagccgtc catttgtaaa gcctttctct gtaataaaag tacaaaattg
     2041 tcaggttttt ttaagttgtg cgaaaacgat agcacaaatc gggcggtaag ttgtttgtct
     2101 gaagttatat tcctgtttat ttgaacgatt tatttcagac ggcgggagga aggcggatac
     2161 ggtatgtagt cggattgaaa tatacggtat gtggggcttt tgggtacaat cgcaacatca
     2221 gtgtttcaag tggggaaggc gatggatgga cggcgttggg cggtatgggg tgcttttgcc
     2281 ctgctgcctt cggctttttt ggcggtaatg gtcgttgcgc ctttgtgggc ggtggcggcg
     2341 tatgacggtt tggcgtggcg cgcggtgctg tcggatgcct atatgctcaa acgtttggcg
     2401 tggacggtat ttcaggcagc ggcaacctgt gtgctggtgc tgcctttggg cgtgcctgtc
     2461 gcgtgggtgc tggcgcggct ggcgtttccg gggcgggctt tggtgctgcg cctgctgatg
     2521 ctgccttttg tgatgcccac gttggtggcg ggcgtgggcg tgctggctct gttcggggcg
     2581 gacggcctgt tgtggcgcgg acggcaggat acgccgtatc tgttgttgta cggcaatgtg
     2641 tttttcaacc ttcctgtgtt ggtcagggcg gcgtatcagg ggtttgtgca agtgcctgcg
     2701 gcacggcttc agacggcacg gacgttgggc gcgggggcgt ggcggcggtt ttgggacatt
     2761 gaaatgcccg ttttgcgccc gtggcttgcc ggcggcgtgt gccttgtctt cctgtattgt
     2821 ttttcggggt tcgggctggc attgctgctg ggcggcagcc gttatgccac ggtcgaagtg
     2881 gaaatttacc agttggtcat gttcgaactc gatatggcgg gggcttcggc actggtgtgg
     2941 ctggtgttgg gggtaacggc ggcggcaggg ttgctgtatg cgtggttcgg caggcgcgcg
     3001 gtttcggata aggcggtttc acccgtgatg ccgtcgccgc cgcagtcggt cgggggatat
     3061 gtgctgctgg cgtttgcggc ggcggtgttg tctgtgtgct gcctgtttcc tttgttggca
     3121 attgttgtga aagcgtggtc ggccggcgaa tcgtggcgtg tgttaatgga aagtgaaacg
     3181 aggcaggcag tgtggaatac tttgcgcttc tcggcggcgg cggtgtttgc ggcggcggtt
     3241 ttgggtgtgg tgtatgcggc ggcggcgcgg cggtcagcgt ggatgcgcgg gctgatgttt
     3301 ttgccgttta tggtgtcgcc ggtttgtgtt tcggcgggcg tgctgctgct ttatccgggg
     3361 tggacggctt cgttgccgtt gctgctggcg atgtatgcgc tgctggcgta tccgtttgtg
     3421 gcaaaagatg ttttatcagc ctgggatgca ctgccgccgg attacggcag ggcggcggcg
     3481 ggtttgggtg caaacggctt tcagacggca tgccgtatta cactccccct cttgaaaccg
     3541 gcgttgcggc gcggtctgac tttggcggcg gcgacgtgtg tgggcgaatt tgcggcgaca
     3601 ttgttcctgt cgcgtccgga atggcagacg ttgacgactt tgatttatgc ctatttgggg
     3661 cgtgcgggtg aggataatta tgcgcgggca atggtgttga cattgctgtt gtcggcattt
     3721 gcggtgtgca ttttcctgct gttggacggc ggcgaaggcg gaaaacggac ggaaacgtta
     3781 taatgcgaac cctttttcag aggacggcaa aatgagcgag ttggataaca tccttgccca
     3841 caaccggcag tttgtcgaat cgggcgaata tgaaaaatac tttaccgaca aataccccga
     3901 acgcgggctg gcggttttgt cctgtatgga tgcgcggatt atcgggctgc tgcccgacgc
     3961 gttgggtttg aaaaacggcg atgccaagct gattaaaaat gccggcgcgc tggttacgca
     4021 cccgtggggt tcggtgatgc ggagcctttt ggttgccgtg tttgaattga aggtcagaga
     4081 gattatggtc atcgcccatc acgattgcgg tatgcagggg ctgaatgccg aagaattcct
     4141 cgggcgcgtc cgggaaagcc ggattcccga agaccgtatc gaaaccctgc gttatgccgg
     4201 tatcgacctc gacggctggc tgaccggttt cgacaacgtc gaagacagcg tgcgccacac
     4261 ggtggacctt atccgtaacc atccgctgat gccgcgccat attgccgttc acggattggt
     4321 catccatccc gttaccggca aactgacgct ggttgtggac ggcagtgttt cagacggcat
     4381 ggacttatcg gaaggaatgg aaacatcatg aagaaaatcg gactgttcgg cggcactttc
     4441 gacccgatac acaacgggca tctccacatc gcccgcgcct ttgccgacga aatcggcttg
     4501 gacgcggttg ttttcctgcc ggcaggcggc ccgtaccaca aagatgccgc ctccgcttcc
     4561 gccgccgacc gccttgccat ggtcgaactg gcgacggcag aagacgcgcg ttttgccgtc
     4621 agcgactgcg acatcgtccg tcacggcgag acttatactt tcgacaccgt ccaaattttc
     4681 cgccagcagt tcccctcggc gcaactctgg tggctgatgg gcagcgacag cctgatgaag
     4741 ctgcatacat ggaaaaaatg gcagatgctc gtgcgcgaaa ccaatatcgc cgtcgccatg
     4801 aggcagggcg acagcctgca caaaatgccg ggcgaactgc acgcatggct gggcaagtcc
     4861 cttcaggacg gcagcgtccg catcttgtcc gccccgatgc ataatgtgtc gtcaacggaa
     4921 atccgccaca accttgccgg ccaaggcgtt tcagacggca tcccgcctgc cgccgcacgc
     4981 tacatccgcg aacacggttt gtatgaaaaa taaagtcaaa tcagtaagga acggctataa
     5041 tgccgtctga aaacatcccg ttaggaacat aatgaacgag caagaactgc aagacctgca
     5101 aaaaatggtc gaggtcgccg tcaacgccct cgaagacatc aaagccaaag atatttccgt
     5161 tctcgaaacg caagacaaaa cttcgctgtt tgctagaatg attatcgcca gcggcgacag
     5221 tacccgccaa gtcaaagcac tggccaacaa cgttgccgtc gatttgaaag aagccggttt
     5281 tgaaatcctc agtaccgaag gcgacagcgg cgaatggacg ttggttgatg caggagacct
     5341 cgtcgtccac gtcatgctcc ctgccgtgcg cgacttctac gacatcgaca ccctctgggg
     5401 cggcgagaaa ccgagtttcc acgccggaat gcagaagccg tggcacgcgg cagactgatt
     5461 cccgatgccg tctgaatgtt cagacggcat tttcctgtca gggagaaagc attgaacatt
     5521 accgttttgg cagtcggcac caaaatgccg cgctgggttg atgaggccgt cgccgaatac
     5581 gccaaacgct tcggacgcga cgtcgcctac gcactcaaag aaatcaaacc cgaaaaacgc
     5641 ggcgcgggcg tgaatgccgc ccaaggtatg gcggcggaag aaaaacgcat cctcgaagcc
     5701 gttccgcaag gcgcgttcct cgtcgttctt gacgaacgcg gcaaagcacc gacctccgtc
     5761 gagctggcgg aacacctcaa aagctggcag caaaacggcg aacacgtctg cttcgtcatc
     5821 ggcggcgcgg acggcatgac cgaccgcctc aaacaacagg cgcgcataat gatgcgcctg
     5881 tccagcctca ccctgccgca cggcatggtg cgcgtccttc tgaccgagca gctctaccgc
     5941 gccgcctcca tcctgcacaa ccatccctat catcgggaat aagaaggctt ttgtccgtac
     6001 ccctaatcgg ttaaaatcac cccgttattt ctgaacacac agcaaaggaa tccgctatgg
     6061 cacgcatggt attttgcgtt aagctcaaca aagaagccga aggcatgaaa ttcccaccgc
     6121 ttcccaacga attgggcaag cgcatttttg aaaacgtatc gcaagaagca tggatggcgt
     6181 ggacgcgcca ccaaaccatg ctgattaacg aaaaccgctt aagccttgcc gatccgcgcg
     6241 cgcgcgaata cctggctcag cagatggagc agtatttctt cggcgacggc gcggatgccg
     6301 ttcagggata cgttccgcaa taacggtttt ccgttttgaa cacaggctgt ccgaaactgc
     6361 ttcagacggc ctttaaaata cgccggacaa ctttatcttc acgcaaccgg acaaattttg
     6421 acattgggca atgttaaaat cccccattat tcccaaccgc gctttcagga gcagatgata
     6481 tgcaacacga cgtttacgac tacaccgcgc atacggtttc taaaaacacc gtcctgcaga
     6541 aaacctaccg cctgctcgga ttttcattca ttccggctgc cgcaggcgcg gcacttgccg
     6601 ccaatgccgg tttcaatttt tacgccgcct tcggttcgcg ctggataggg tttgccgtcg
     6661 tgttggcgtt tttctacggt atgatccact tcatcgagaa aaaccgttac agcaataccg
     6721 gcgttaccct gctgatggta ttcacattcg gtatgggcgt attgatcggc cccgtgctgc
     6781 aatacgcgct ccatattgcc gacggtgcga aaatcgtcgg cattgccgcc gcgatgaccg
     6841 ccgccgtctt tttaacgatg tccgccttgg cgcgccgaac ccggctcgat atgaacgcgc
     6901 tcggacgctt cctgaccgta ggtgcggtca ttctgatggt cgccgtggtt gccaatctgt
     6961 ttttgggtat tcccgcactc gccctgacca tttccgccgg ttttgtcttg ttcagttcct
     7021 taatgattat gtggcaggta cgcaccgtca tcgacggcgg cgaagacagc cacatcagcg
     7081 cggcactgac actgtttatc tcgctttaca acatcttcag cagcctgctg aacatcctgt
     7141 tgtctttaaa cggcgaggat tgatgccggt aaaacgccgt ccgactatgc cgtctgaaaa
     7201 tgcttcagac ggcattttac tttgggcata caatacggat gcacacgcac caacacacag
     7261 ccgactatga agccgggagg aatcaaatgt taccgaatac accgcgccgc gccgtttatg
     7321 ccggcagttt cgatccgccc acattggggc atctgtggat gatacggcag gcgcaatcta
     7381 tgtttgacga actcatcgtc gccatcggca ttaaccccga caaacgcagc acctataccg
     7441 tcgctgaaag gcaggatatg ttgtgcgata ttactaaaat gtttcccaac gtcagaaccg
     7501 atgtatttga aaaccgattt ctggtgcatt acgcccgtga ggtagatgca ggattcatcg
     7561 tgcgcggcat ccgttctgct tcggattacg aatacgaacg ttccatgcgc catatcaaca
     7621 gcgacctcgc ccccgaaata tccaccgtat tcctcatgcc gccgcgcgaa atcgccgaag
     7681 tgtcgtccac tatggtcaaa ggactggtcg ggccggaagg ctggacggaa acgatccacc
     7741 gctacgtgcc gcaagctgtg tacgaaaaaa tccttgccga acatcaacac gaaaattgaa
     7801 cccttctatt tttgcaaggc atatatggat atgttggaaa ttttgttccg ccatcaagac
     7861 ttcgtcgcca tcaacaaacc gggcggcata tccgtccacc aagacagcgg cgagaccgga
     7921 ctggcccgaa cactcgccat acagttgggc gtagagcgtg tgtggctgct gcaccgattg
     7981 gacaaacaga ccagcggcat tttactgttt gccctcaaca aagaaagcgc gtccgccctt
     8041 agcggacagt ttgccggcaa aagcatcaaa aaaacctatt tggcactgtc cgaccgcaag
     8101 ccgtccaaaa agcagggttg gattaaaggc ggaatggaaa aatccagatg tggaatgtgg
     8161 aagttgacgc gcaatacaga aaatatcgcc gttacccgat tccacagcat cagtatcgcc
     8221 gaaaaactgc ggctgttcat cctagaaccg catacgggca aaacgcacca attgagagtg
     8281 gcgatgaaaa gtttgggcag tccgattctg ggtgacagct tatacggcgg aacagaatcc
     8341 gaaaccatgt ttctgtatgc atggaaaata caatttgact accaaaaccg acaaatcgaa
     8401 attgttgcac ccttaaaaaa cgaatggcag actgaaaata tccatcgggc actggaagaa
     8461 ttctgtatgg aaaataaaat tgactgatat aaattaaatt tataaacaaa tagataaata
     8521 tttttaaaaa aatttcttca gaaaag
//
LOCUS       DAWKQB010000088         8463 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.088, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000088 DAWKQB010000000
VERSION     DAWKQB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.088"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJL14_001443"
     CDS             complement(208..1593)
                     /gene="pntB"
                     /locus_tag="WJL14_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ7656501.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1659..2042)
                     /locus_tag="WJL14_001444"
     CDS             complement(1659..2042)
                     /locus_tag="WJL14_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ7656502.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLAGLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2046..3587)
                     /locus_tag="WJL14_001445"
     CDS             complement(2046..3587)
                     /locus_tag="WJL14_001445"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ7656503.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEDELPLLKEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIASINIFGGFAVTRRMLN
                     MFKKG"
     gene            3877..4710
                     /gene="serB"
                     /locus_tag="WJL14_001446"
     CDS             3877..4710
                     /gene="serB"
                     /locus_tag="WJL14_001446"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ7656504.1"
                     /translation="MPHALVLQFPSAAALPSDFPLRLPEPDCADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLPDMDFDELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDIPMLKETGIGVAYRAKPKVRAAADACINFGGLERVRGLF
                     R"
     gene            4776..5998
                     /locus_tag="WJL14_001447"
                     /pseudo
     CDS             4776..5998
                     /locus_tag="WJL14_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6159..7751
                     /gene="purH"
                     /locus_tag="WJL14_001448"
     CDS             6159..7751
                     /gene="purH"
                     /locus_tag="WJL14_001448"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ7656505.1"
                     /translation="MSVIKRALISLSDKTGAVEFAQTLHKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGQPQIGEFPSRFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLQISLTTPLEAGANRFELKRVGGGLLVQTP
                     DIHRLNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMS
                     RVDSTRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQE
                     VFDAADEHGIAMVVTDVRHFRH"
     gene            8349..>8463
                     /locus_tag="WJL14_001449"
     CDS             8349..>8463
                     /locus_tag="WJL14_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ7656506.1"
                     /translation="MLIHYIDIAKRNFVIAVSSLSKTKTETNNPKGIAHTIE"
BASE COUNT         1894 a         2310 c         2357 g         1902 t
ORIGIN      
        1 taaaaatcca tcttcaacac ggagatggat tttgtttgtt tcgttgattt tgtgtcagtt
       61 tcagatgtag gtgcttattc ggacgggccg tctgaaaatg tttgccccaa tgcaaaaaaa
      121 atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa acagattgaa
      181 aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa tgtcatccac
      241 ggttttcttc gcatctccga aacacattac gctgttttcg ttgaagaaca gcgggttttg
      301 tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt ttgccttcca
      361 tacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg cggcaggatt
      421 gacggtatcg ttcgcaccaa tcaccaagac cacatcggtt tcggggaagt cgtcgttgat
      481 ttcgtccatt tccaacacga tgtcgtaggg gactttggct tcggcaagca gtacgttcat
      541 atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttctg tgccgttttt
      601 acgcaaaagc tcggtgattt cggcaacggg gtattgcgct tgtgcgactg ccataccgta
      661 gcccggggta atgatgacac tgtttgcgcc tttcagcatt tcggcaacat cggcagcttt
      721 gacttctcgg tattccccta tctcttggct gccggaagat gatgtgccgc tgtcgctgcc
      781 gaaaccaccg gcaattaccg agacaaacga gcggttcatg gctttgcaca taatgtagga
      841 cagaattgcg ccgcttgagc cgaccagcgc gccggtaacg atgagcaggt cgttggagag
      901 cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatag atacgaccac
      961 gggcatatct gcgccgccga tggaggcgac caagtgccag ccgaatgcga gggcgatcag
     1021 agtcattatc agcaggatga agccgctgcc gtcaatgccg acaaatacga gcagcaacac
     1081 aaacgatacg ccaagtgcca gtgcgttgag cttgtgtttg gcgggcagtt gcagcgggct
     1141 gctgctgatt ttgccgttga gcttgccgaa tgcgaccagc gagccggtaa aggttaccgc
     1201 gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat cgtgcgaaac
     1261 gttgcccggc gcgatatagc tgttgaagcc gaccaaaacc gccgctaggc cgacgaagct
     1321 gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg ctttgtagat
     1381 gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt gggtattgtc
     1441 ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat agcagccctg
     1501 tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa ttgcggcaac
     1561 gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt cgatgatttg
     1621 ttttcaatgc cgtccgaact gttcagacgg tatctgtttc aagcagccgc gacaaacagc
     1681 ccgacggtgc aggcaaatcc gcctgcccac atcagtgagc gcatagcggc tttgtcggcg
     1741 atatagcacc agataaaggc gaggcggaac aggatgaaca ggcccgcaag cgtgttgatg
     1801 gtcgattgcg ccgcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc aaacggtgca
     1861 aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc ttgcgtgtgc
     1921 gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc ttttttggca
     1981 tacgccgcac aaaaaagcgg caataggcag gcaatcagaa tgcaccaata ggcgaaagtc
     2041 atggcttacc ctttcttaaa catattcagc atccggcgtg ttaccgcaaa gccgccgaag
     2101 atgttgatgc tggcaatcag gatggcaaca aacgacagca gcgaaacaaa gccgttgccc
     2161 tgaccgattt gcagcagcgc gccgacgacg atgatgccgg aaatggcgtt ggtcaccgac
     2221 atcagcggtg tgtgcagcga gtggctgacg ttccaaacga catagtagcc gatgacgcag
     2281 gcgaggacga acacgataaa gtggttcagg aatgctgcgg gtgcgaccgc gccgacccac
     2341 agtaccaaca cggcggcgat gacggcgggc gcgagttttt tccacagggg aacgggtttc
     2401 ggttcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg ctgggcggaa
     2461 acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgt gggtaacggt catgttgcgg
     2521 ataatcacgt cttcgaagtc caacgtgatt tcgccgtctt tgttcgggct taacagcttg
     2581 gtcaggttga ccaagttggt ggcgtaaagc tgggaagact gtccggcaag gcggtttgcc
     2641 atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc gcccgggcgg
     2701 gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga gccggatttc
     2761 atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg aatggcggcg
     2821 gtagtgatga tgatgtccac ttctttcgcc tgctcggcaa agagtttcat ctcggcggcg
     2881 ataaattcgt cgctcatcac tttggcgtag ccgtctccgc tgccgcccga ttcttgcggg
     2941 aagtcgagtt tcaaaaactt gccgcccatc gattcgattt gttccgccac ttccaagcgg
     3001 gtatcgaacg cgcgcaccac tgcgccgagc gagtttgccg taccgatcgc cgccaaacct
     3061 gccacacctg cgccaatcac caaaacctgc gcgggcggca ctttgccggc ggcggtgatt
     3121 tggccggtga agaaacggcc gaaggcgttg gcggcttcaa tcacggcgcg gtagccgctg
     3181 atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg gggcaccatg
     3241 tccattgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc ctcgttttgg
     3301 cgcggccaca ggaagctgac gatggtctgt ccttctttga gcagcggcag ctcgtcttcg
     3361 gacggcgcgt taaccttata aatcaaaggg cagacccaaa ccgccgcttt gtcggcaacg
     3421 gttgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc tgcaccgctt
     3481 tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg cgtacaggcg
     3541 acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc tgaatccttt
     3601 ttcgggttgt ttatatgtat cgtgggttaa atttaaatcg gggcggggcg gagcaacgcc
     3661 gtaccggttt aaagccgact cacttcaaat gttaatatat tttagataat ccccttataa
     3721 cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg acacatactg
     3781 ccgtttgtgt ttgaagtcgt tttccgattg cagtcccgcc cgccgtaaca ttctttgccg
     3841 tttcactata taatccgcat tttttgagcc gccgttatgc cgcacgccct cgtcctccaa
     3901 tttccctccg ccgcagccct gccttccgac ttccccttac gcctgcccga acctgattgc
     3961 gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag cgaaaaagac
     4021 gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgccggatat ggatttcgac
     4081 gaactcggtt tgattgtcag cgatatggat tcgacgctga ttaccatcga atgcgtcgat
     4141 gaaattgcgg caggcgtggg tttaaaagac aaggtagcgg aaattaccga gcgttcgatg
     4201 cgcggcgaac tcgatttcga acagtcttta cgcagccgcg tcgcgctgtt ggcgggattg
     4261 gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc cggtgcggaa
     4321 tttttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc gggcggcttc
     4381 acgtttttta ccgaaaggct gcaacaacgc ctcggcttcg aataccagca cgccaatgtt
     4441 ttggaaattg aaaacggcag gctgaccggc cgtctgaaag gtagaatcat cgacgcgcag
     4501 gcaaaggcag atttattgct cgaataccgc agccgcctcg gattgcagcc acatcaggtg
     4561 ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaaacggg cataggcgtg
     4621 gcttaccgtg ccaaaccgaa agtgcgggca gccgccgatg cctgtatcaa tttcggcggt
     4681 ttggagcgtg tacgcggcct gttcaggtag gcggatagga aacggatgtc gtctgaaaaa
     4741 cgtgttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa ctttggtttg
     4801 ccctggcagc agcaggcgtt atcggcggtt tggtcggcat tgtgctgacg gaactgatgc
     4861 acttcataca gcatacggca tacggttatg gcgcggacgg tgcgtacatc tcgttccgcg
     4921 aaggtgtggc ccgtgcttcg gaagggcggc gtttgggcgt attgatcctg tgtggtatgt
     4981 tggcaggcgg cggctggtgg ttgctcaaac gtttcggcaa gccgcaaatc gaaatcaaag
     5041 ctgccttgaa acagccgttg caggggctac cgtttctgac gacggttttc catgttctgc
     5101 tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcg ccgcgcgaaa
     5161 tgaccgccgc gtttgcttcg gcaggcggca aacgcttggg tttggatgaa ggcgaaatgc
     5221 gcctcttgat tgcctgtgcg tccggcgcgg gtttggcggc cgtgtataac gtgccgctcg
     5281 cctccacgct gtttatcctc gaagcgatgc tgggcatgtg gacgcagcaa gctgtcgccg
     5341 ccgcgttgct gacttcggtc attgccaccg ccgtcacacg catggcttag gcgacgtgca
     5401 gcaatatcat cccgccaacc ttgccgtaaa tacttcattg ctttggtttt ccgccgtcat
     5461 cggcccgata ttgggcgcag ccgccgtctt tttccagcgt accgcccaaa agttcccctt
     5521 catcaagcgc gacaatatca aaatcattcc cttggcagtc tgtatgtttg cactcatcgg
     5581 cgcggttgcc gtgtggtttc ccgaaatttt gggcaacggc aaagcaggca accaactgac
     5641 ttttggcggt ttgaccgact ggcaatacag ccttgagctg accgccgtca aatggctggt
     5701 cgtcttgatg gcgttggcgg caggcgcata cggcggcctg attacaccgt cgatgatgct
     5761 cggcagcacc gtttcttttg ctgccgcagc cgcgtggaac agcgtcttcc ccgaaatgcc
     5821 gtctgaaagc gcggcagtcg tcggagcggc ggtgtttctc ggcgtatcgc tcaaaatgcc
     5881 gctgaccgcc attgtgttcg tattggaact gacctacgcg ccgctgtccc tgctgctgcc
     5941 tttgtgcatc ggtatggcgg gggcattggg aacatcggga aaaatgggct tcaaatgaat
     6001 gccgacaaat gccgtctgaa aggttttcag acggcatttc tttgtaaatt gtcaacaata
     6061 tctgcaaaaa cttactgcca aaaaactgaa aatagcggat aataccgccc cgaaaaagtt
     6121 cccctttccc catccaactg tcagaaaagg aagccccaat gtccgtcatc aaacgcgccc
     6181 tgatcagcct atccgacaag acaggcgcag tcgaatttgc ccaaaccctg cacaaactcg
     6241 gtgttgaaat cctctctacc ggcggtacag caaagctctt ggctgatgca ggcgttcccg
     6301 ttatcgaagt tgccgactat accggttttc ccgaaatgct cgacggccgc gtgaaaacct
     6361 tgcatccgaa aatccacggt ggcatcctcg gccgtcgcga tttgggcgaa cacgtcgcca
     6421 agatggaaga acacggcatc ggcaacatcg acctcgtgtg cgtcaacctc taccccttcg
     6481 ccgccaccat cgccaaacca aactgcacgc tggaagacgc gattgaaaac atcgacatcg
     6541 gcggcccgac tatggtgcgc tccgccgcga aaaactggaa acacgtcgcc atcgttaccg
     6601 acaccgccga cttccccgcc attgccgccg aactcgaagc caacaacggc gcgttgagcg
     6661 acaaaacccg tttcaacctc tcacgcaaag cgttcagcca caccgcccaa tacgacggca
     6721 tgatttccaa ctatttaacc tcgctttcag acgacgtttt gagcggccag ccccaaatcg
     6781 gcgaatttcc cagccggttc aaccaaagct ggattaaagt gcaagacatg cgctacggcg
     6841 aaaatccgca ccaacgcgcc gcgttctacc gcgatgtata ccccgccgca ggcagccttt
     6901 ccgcctacaa acaactgcaa ggcaaagagt tgtcgtacaa caacattgcc gatgccgatg
     6961 ccgcttggga agccgtcaaa tcctttgacg cgcccgcctg cgtgattgtg aagcacgcca
     7021 atccttgcgg tgtcgccgtt gcagccgata ccttgaccgc ctacaaactc gcctacgcta
     7081 ccgacactac cagcgcattc ggcggcatca ttgccttcaa ccgcgaagtg gacggcgaaa
     7141 ccgtcaaaca aattaccgac aaccagttta tggaagtctt gatggcgccg aagtttaccg
     7201 ccgaagccct cgaaatcgct gctgccaaga aaaacgtgcg cgtattgcaa atttctctaa
     7261 caaccccgct cgaagcaggt gccaaccgct ttgaactcaa acgcgtcggc ggcggattgt
     7321 tggtgcaaac ccccgatatt caccgcctca accgcgccga tttgaaagtc gtttccaaac
     7381 gccaaccgac cgagcaagaa tggaacgacc tgatgtttgt ttggaacgtc gcaaaatacg
     7441 tcaaatccaa cgccatcgtc ttcggcaaag gcggtcaaac ctacggcatc ggcgcaggcc
     7501 aaatgagccg cgtggacagt acccgcatcg ccgcccgcaa agcgcaggac gcaggcttgg
     7561 acttaaacgg cgcatgtgcc gcctctgatg ctttcttccc cttccgcgac ggcgtggacg
     7621 tgattgccga gcagggcatc aaagccatca tccatccggc aggttcgatg cgcgatcagg
     7681 aagtcttcga cgcagcggac gaacacggca tcgccatggt cgtaaccgac gtgcgccact
     7741 tccgccatta acgcagatga acaaggcaat gccgtctgaa gggctttcag acggcatttt
     7801 gcgctatttt gtgaaggtag ggatgacggt tcgggtattc ctgacagggt ggattttcaa
     7861 ggtgttgtat agggtgtagg aggattcgta aaaggtggga tgcagggtgt gcttcagccc
     7921 gctgcatcaa aaatttttgg agaatcggcg ggagtcggcg gttttggttt cggcggggac
     7981 ggtggaaatg ggtaacattg acggaatcga cggaagcggt gggcttcagc ccaccgcttg
     8041 tatattggaa tcaccgtatc atagcgccac ccgcgcccac ccaaggcaca caaccgttgc
     8101 gtagctcagg gagcggcagg gcaacccatc gacacaaccg gacagttgcc ggacaacaca
     8161 accgaatgca aggcaggttt atgatgagta cccaatacca ttacgcaggt atagtgaatt
     8221 aaatctaaac cagtacagcg ttggttcgcc ttagctcaaa gagaacgatt ctctaaggtg
     8281 ctgaagcacc aagtgagtcg gttccgtact atccgtactg tctgcggctc gccgccttgt
     8341 cctgattttt gttaattcac tatatcgaca tcgccaaacg aaacttcgtc atcgccgttt
     8401 cgtctttgtc taaaaccaaa accgaaacca acaaccccaa aggtatcgcc catactatcg
     8461 aat
//
LOCUS       DAWKQB010000089         8406 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.089, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000089 DAWKQB010000000
VERSION     DAWKQB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.089"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJL14_001450"
     CDS             complement(308..1240)
                     /gene="cysK"
                     /locus_tag="WJL14_001450"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ7656507.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1434..2039)
                     /locus_tag="WJL14_001451"
     CDS             complement(1434..2039)
                     /locus_tag="WJL14_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656508.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJL14_001452"
     CDS             complement(2036..2887)
                     /gene="dapF"
                     /locus_tag="WJL14_001452"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ7656509.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            3024..3320
                     /locus_tag="WJL14_001453"
     CDS             3024..3320
                     /locus_tag="WJL14_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656510.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGFYVPDCRKSSLSPKNI"
     gene            3334..4002
                     /locus_tag="WJL14_001454"
     CDS             3334..4002
                     /locus_tag="WJL14_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ7656511.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(4075..6198)
                     /gene="pnp"
                     /locus_tag="WJL14_001455"
     CDS             complement(4075..6198)
                     /gene="pnp"
                     /locus_tag="WJL14_001455"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ7656512.1"
                     /translation="MMFDKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVA
                     VTTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRP
                     LFPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYING
                     VYVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAIN
                     AINEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWS
                     AVKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTG
                     VLPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEV
                     GRMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCL
                     SLLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQ
                     MDIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKI
                     REVIGKGGETIRAITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGK
                     VYEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEV
                     DDRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6432..7295)
                     /locus_tag="WJL14_001456"
     CDS             complement(6432..7295)
                     /locus_tag="WJL14_001456"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ7656513.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7493..8356
                     /gene="rfbD"
                     /locus_tag="WJL14_001457"
     CDS             7493..8356
                     /gene="rfbD"
                     /locus_tag="WJL14_001457"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ7656514.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         2001 a         2440 c         1943 g         2022 t
ORIGIN      
        1 gtggattaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
       61 cggcttcatt gccttgtcct gatttttgtt aatccactat atctgtactg tctgcggctt
      121 cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa tatttccctc aagcgatgtg
      181 gcggcaccaa cacctgatta aaacacaacc ggcgttttcg gcatatacgc aaaaacgcat
      241 tttccggcac ggcgttacac cgataccgaa aaatgcgtct gaatgtttcg acaatccgat
      301 taaagcatta tgccaaatct gcaaaaagtg gcgtagagag atagcgttcg ccataagaag
      361 gcagcagcac gactatcagc ttgccttcgt tttcaggctg tttggcaagc tgcaacgcac
      421 tccaaaccgc cgcaccggaa gaaataccca ccaaaatgcc ttctttttcc gctattgcgc
      481 gggcggtttc aaaagccgct tcgttcgaca ctttggtaat gctgtcgtaa attttggtat
      541 tcaaaacggt cggaataaag cctgcgccga tgccttgaat cgggtgcggg cctttttcgc
      601 cgccgctcaa tacgggtgaa gcttcaggct cgacggcaac cactttaact tcgggtttgt
      661 attttttcaa cacttcgccc acgccggtaa tcgtaccgcc cgtgccgacg ccggcaacga
      721 agacatcgac tttgccgtcc gtatcccgcc aaatttcctc ggcggttgtt ttgcggtgga
      781 cttcggggtt tgcctcattg tcgaactggc gcggcataaa ataagtgtcg ggatgcgcgt
      841 ccaccaagga tttcgctttg gcaatcgcgc ccgccatacc ttcggcggca ggggtcagaa
      901 tcagctccgc accaaacgcg cgcaacagca ttttgcgctc cttactcatg ctttccggca
      961 tggtaatcgc cagcttgtag ccgcgtgcgg cacataccat tgccaaaccg acacccgtat
     1021 tgccgctggt tgcttcgaca atgacggtgt ttttgttgat tttgcccgct ttttcggcac
     1081 cctcgatcat tgcttcggca atgcggtctt tgacgctgct gcctggattg aaaaattcca
     1141 gtttcacggc aacctccgcc ttcaaacctt cggtcagacg gttcagtttg accaaaggcg
     1201 tgttgccgat tagttcggtg atgctgtttg caattttcat cttctgctcc tgaataataa
     1261 ggtttcgaca gtaaggcgca atccccatag gcatcctgtc cgattgcagg ggatttgcgc
     1321 cgaaacataa acaaatttcg gatatggctt tatcctaaac ctatgccgcc taaaaaacca
     1381 atatccaaaa tttctttctt tataactttt tgttccgata cggccgcacc gtattatctt
     1441 ccaatttctg aaagcagcag caccagtacc gagacctgca tcaacgctgc cgtcaacccg
     1501 ccgagtcgca gctgcgagcc gccgaacgtt gacggcggct gccggtctat accgcagcgg
     1561 tggacatagg caaccgccat cagcacaaac tgcaacacga accaataaac cggcttcagc
     1621 gcataaagtt tgagcagtgc cgcaaacaca aacacttgaa ccgttcccga aaagcaataa
     1681 tgcaccaacg cacatattcc gacaaaagca agcgtcatcg atacattact gacggcaaac
     1741 agcccgagca gaggatgttc ggggtctgcc tgccatccgt ttccatccgt tttccggttc
     1801 caatgcccga tgaaaaaaaa tatgctgccc aaagtcaggt aaaaatgggg gatgaacaag
     1861 ggcgcggcgc gggtcattcc ccatatgccg ggcatcagct ttgcccccaa aaccgatatg
     1921 cccagccaca gcataatact cgcccaaaac cattgcaaca ttcctgcgcg gaatatcaga
     1981 aacagaaaaa ccgccgcata cagtaaggca gacaaaaaat ccaaatgcag caaaatcatg
     2041 aatacgccaa ctcgccttca aacaccgttt ccgcagggcc ggtcatcatc acatcgccgc
     2101 cgcaggccca ttcgatatat aaagttccgc ccggcaaaat cacctctacc gttttccctt
     2161 catccaacaa ccccagacgg atacccgcca ccacagccgc acacgcgccc gtaccgcacg
     2221 cttgggtttc gcccacgccg cgctcgaaca cgcgcaaacg aatcgcggtt cggctgacaa
     2281 tctgcataaa gccgacgttg acgcgttcgg gaaactgcct gtgcggttcg ataagcgaac
     2341 cggtttcgcg cacctgcgcg cattctacgt catcgaccac aatcaccgca tgggggttgc
     2401 ccatattgac gcagctgaca ggctgaatgc cggattcgag atgcaccccg taaatacagg
     2461 catcatcccc ctcgcccgat tcggggacaa acggtatttc agacggcata aacctcggtt
     2521 tgcccatatt gaccgtaacc atgccgttat cggacaattt cggaaaaata acgccatttg
     2581 ccgtttcaac acaaatttct ttcttatcgg tcaaaccctt gtctgcaaca aaacgggcaa
     2641 aacaacgcgc cccgttgccg cactgcccga cctcgctgcc gtcggcattg aaaatacggt
     2701 aacggaaatc cacgccttcg gtttccgaac gcccgaccac caaaagctgg tcgaagccca
     2761 cgccccggaa acggtccgcc catgccgcaa tcggcgcgtc ctcgggggta aaatcctgac
     2821 tgaccgcgtc aatcaccata aaatcgttgc ccaaaccgtg cattttggta aatttcaaag
     2881 tgttcatatt gttccgaaat cccgtatcgt ctgttgcttc cgaatccgaa atcccccgca
     2941 ccgaagggaa atgcttccat ctcccgccgc ctgaggatta aaatcagcca ttatactgtt
     3001 tgttacccct tacaagaaac accatgttcc gcatattact gttgcttttc ccttgtgcaa
     3061 tccttgccgc ctgcacccga aacataccgg caaacccggc cgtttcaaat tgcgcgcatc
     3121 ttcaaaaccg caccatcctg tattttgacg cacacggcaa acccgccgac ggcgcagtaa
     3181 agggcggatt ttacaaaatt atcgaaatac agggtgcaaa ccgcatcctt attcaagact
     3241 ttaacagcac gggcaatgcc ctcggcggat tctatgtgcc ggactgccgg aaatcatccc
     3301 tttcaccgaa aaatatctga cttcaaactg cctatgaacc acgacatcac tttcctcacc
     3361 ctgttcctac tcggtttctt cggcggaacg cactgcatcg gtatgtgcgg cggattaagc
     3421 agcgcgtttg cgctccaact ccccccgcat atcaaccgct tttggctgat cctgctgctt
     3481 aacacaggac gggtaagcag ctatacggca atcggcctga tactcggatt aatcggacag
     3541 gtcggcgttt cactcgacca aacccgcgtc ctgcagaata ttttatacac ggccgccaac
     3601 ctcctgctgc tctttttagg cttatacttg agcggtattt cttccttggc ggcaaaaatc
     3661 gagaaaatcg gcaaaccgat atggcggaac ctgaacccga tactcaaccg gctgttaccc
     3721 ataaaatcca tacccgcctg ccttgcggtc ggaatattat ggggctggct gccgtgcgga
     3781 ctagtttaca gcgcgtcgct ttacgcgctg ggaagcggta gtgcggcaac gggcgggtta
     3841 tatatgcttg cctttgcact gggtacgctg cccaatcttt tggcaatcgg cattttttcc
     3901 ctgcaactga aaaaaatcat gcaaaaccga tatatccgcc tgtgtacggg attatccgta
     3961 tcattatggg cattatggaa acttgccgtc ctgtggctgt aagccccatt cattcccgat
     4021 acccgtaaaa aataccgtct gaaacctgtt cagacggcac tttcacccta agcgttactc
     4081 ggcggcattt tcctcacggg caggcgcgtc cagcagggct ttgatggaca gacggacacg
     4141 gcctctgtcg tccacttcca atgctttcac gttcaccacc tgaccgactt gcaggtagtc
     4201 gccgacattg cgtacgcgct cgtgggcgat ttggctgatg tgtaccaaac cgtctttgcc
     4261 cggcatcaca ctgacaatcg cgccgacatt gttgtcgagg attttcacca cagtgccttc
     4321 gtacactttg cccacttcca cttcggcagt aatctgctcg atgcgttttt tcgccgcatc
     4381 gccggcttct tgagtggttg cggcaatggt aatcgtaccg tcttcggcaa tattgatttc
     4441 cgtaccggtt tcagcggtaa tcgcacggat ggtttcaccg cccttaccga taacttcgcg
     4501 gattttgtct tggttgattt tcatcgtgaa caagcgtggc gcgtgtgcgg acagctcttg
     4561 cgggcccgca acggcggctt tcatctgatc caagatgtgc agacgcgctt ctttggcctg
     4621 cgccaaagcg atttgcataa tctctttggt aatgccttgg attttgatgt ccatttgcag
     4681 cgcggtaacg ccttcggtcg taccagccac tttaaagtcc atatcgccca agtggtcttc
     4741 gtcgcccaaa atgtcggtca ggacggcaaa cttattgcct tccagaatca gacccatcgc
     4801 gataccggca acgtgtgctt tcaaaggcac gccggcggac agcaggctca agcagccgcc
     4861 gcagacggaa gccatagagg aagagccgtt ggattcggta atttcggaga ccacgcgcat
     4921 ggtgtagctg aaatcttcag gtttcggcaa tacggccaac aatgcacgtt tagccaaacg
     4981 accgtgaccg atttcacggc gtttcggcgc gcccatgcgg cccacttcgc cggtagagta
     5041 cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat tcgccggaca gcgcgtcgat
     5101 gatttgctcg tcgcgcgaag tacccaaagt cgctacggca agggcttggg tttcgccacg
     5161 ggtaaacaat gcggaaccgt gcgtgcgcgg caatacgccg gtttggatgt tcagcggacg
     5221 gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggccg tccaaaattt ggctgcggac
     5281 gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg ttggctgcca gcgtgtcggt
     5341 ttcttcggta atcaaggctt cttttaccgc actccaagct tcgtccaatt tggcagaacg
     5401 cgcttgtttt tgacggattt tgaacgcttc tttaatggtt tcgccggcaa tcccgcggac
     5461 tttggcaacc agttcctcat tggtttcagg tgctttccaa tcccaaagtt ccggattgac
     5521 ttcgtcggca aattcattga ttgcattgat ggcaacctgc atttgatcgt ggccgtaaac
     5581 caccgcgccc agcatcacgt cttcgggcag gattttggct tcggattcca ccatcaacac
     5641 ggcttttgaa gtaccggcga ccaccaagtc caattgcgat ttcgccaatt cggctttagt
     5701 cgggttcaaa acgtacacgc cgtttatata acccacgcgt gccgcaccaa tcggaccggc
     5761 aaacggtacg ccgctcaaca ccagcgcggc agacgcgccc aacattgcag gaatgtcgga
     5821 atcgatttcg ggatcgacgg acacgaccat cgctacgatt tggatgtcgt ggtagaaacc
     5881 ttcggggaac agcggacgaa tcgggcggtc gatcagacgg ctggtcagga tttctttttc
     5941 gctttgtttg ccttcgcgtt tgaagaaacc gccggggatt ttacctgcgg cgtaagtgcg
     6001 ttccaaataa tcgacggtca gggggaagaa gtcttggcct tctttcactt ctttgttggt
     6061 ggtaacggca accaaaacca cggtgtcgcc catagagact ttaacggcag cggcggcttg
     6121 gcgggcaatt tcgccggttt ccaaagtaac ggtctgatta ccgtattgga aggtcttaac
     6181 gtgtttgtcg aacatcattg ttcctttcaa aataccgcac tgctaaaaca ctaataatgc
     6241 acactaaaat ccgaatgtgc atagttaggg tttcagaccg tgcggcaggt tgtaaacaag
     6301 ctttcagacg acatttcgga cgctgaaagc aattccagat tataaaggca accatcctta
     6361 aaatccagta ttgatacaat aaaaaggccg tctgaaccat atttctttca gacggccttc
     6421 aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt cccgatactt ctcgacagtt
     6481 ttctgaatca catcggcagg cactttcggc gcaggggctt ttttgttcca accgctttgt
     6541 tccagccagt cgcggacgaa ttgtttgtca aaagacggcg gattggtgcc gacttcatat
     6601 tgatccgccg gccagaagcg gctcgaatcg ggagttaata cctcatccat cagcgtcagc
     6661 gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc aaataataat accgcgcgat
     6721 ttggcatatt ccgccgcttc ggtgtaaagc cgaaccgcct tggcgcgcac ttcttccgcc
     6781 aattctttgc cgataatgcg tccgcattct tcaaagctga tgttttcatc gtgatcgccg
     6841 actgcggctt tggtcgatgg cgtaaaaatc acttcaggca gttgttgcgc ttcctgcata
     6901 ccttcgggca gccgaatacc gcaaaccgag ccggtttttt gataatcttt ccaaccgctg
     6961 cctgccagat aaccgcgcac aatcgcctct actttcaccg gagtaagctt tttagccacg
     7021 acggcgcgtt tttctaaaac tttggcttcg ttttcaggca aaacatcgta aaccgtttga
     7081 ccggtaaagt ggttgggcat aatatgcgcc agttttttaa accaaaaatt ggaaatctgc
     7141 gtcagaatct cccctttgct cggaatcggg tcgtccaaaa tcacatcaaa cgcggacagg
     7201 cggtcggaag cgaccatcag catacgttta tcgtcgattt catataaatc gcgcactttt
     7261 ccagaataga tctttaccaa accaatctca ctcatttcgc ccccctgaaa atatcttgaa
     7321 aataccgacc cgacacccga caggtttgaa tcgcaaaccg atattctagc cgaagtcggc
     7381 gcaaaacaat acccatggca caaaaagcca acccgtcaac cgtcggcaaa gttttggcac
     7441 tataataccg acagcaagtc ctacaataca cttttaccaa aggaaatacc tcatgagaat
     7501 cctattgaca ggctcgaaaa gccaactggc acgctgcctg cgcgaccgtc ttccggaaga
     7561 ctgggaaacc attgcgacgg attccgcatc cctagacatt accgatgccg atgccgtctg
     7621 caacatggtc aaaagtttcc aacccgacgc cattgtcaac acggctgcct atactgccgt
     7681 cgacaaagcg gaaggcgatg cggcagcggc atttgccgtc aatgcttccg ccgtttacaa
     7741 ccttgccttg gcagcacatc gcgcccatgc ccgattcatc cacatctcaa ccgactatat
     7801 ctttgacgga aaagggaaaa gaccctacca ggaaagcgac tttaccaacc cttccaatgt
     7861 atacggacaa tccaaaaccg ccggcgaact gctcgcactg tctgccaatc ccgacagcct
     7921 tatcctgcgg acttcttggc tgtttagcga atacggggac aactttgtcc gcaccatgct
     7981 gaaccttgcg cgggaacgtt ccccgctgtc cgccgtccac aaccaaatcg gctgcccgac
     8041 ctatgccggc gacttgtccg ccgccatcat ccgcctgttg cagcagtcca atcccgttcg
     8101 cggcatttac cactacgtcg gcggcaaatc cgtatcctgg tacgaatttg cccaacatat
     8161 tttccaagcg gcatcgcaac agcagacatc cttccccgtt cccgaattga ctgccgtttc
     8221 agacaaggaa tatccgaccg ccgcccccag gcccgcatac agcattttgg actgccgcaa
     8281 aatcgaaaac gacttcagca tcaaaccgtc agactggcaa aaagcccttg cacaggtcgt
     8341 ttccaagctg ctctgatgcc gcccgcctct ccgtttccgc cgtcaagcac cgccttggcg
     8401 gttttc
//
LOCUS       DAWKQB010000090         8352 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.090, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000090 DAWKQB010000000
VERSION     DAWKQB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.090"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(145..957)
                     /locus_tag="WJL14_001458"
     CDS             complement(145..957)
                     /locus_tag="WJL14_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ7656515.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGVDWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTILAGREVPHLLAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
     gene            1042..1914
                     /locus_tag="WJL14_001459"
     CDS             1042..1914
                     /locus_tag="WJL14_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ7656516.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLADILTADDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            complement(1971..3125)
                     /locus_tag="WJL14_001460"
     CDS             complement(1971..3125)
                     /locus_tag="WJL14_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ7656517.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(3170..3538)
                     /locus_tag="WJL14_001461"
     CDS             complement(3170..3538)
                     /locus_tag="WJL14_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7656518.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            3712..4155
                     /locus_tag="WJL14_001462"
     CDS             3712..4155
                     /locus_tag="WJL14_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ7656519.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLASPPERINIGAVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            complement(4282..4902)
                     /locus_tag="WJL14_001463"
     CDS             complement(4282..4902)
                     /locus_tag="WJL14_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ7656520.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RECLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(5260..6459)
                     /locus_tag="WJL14_001464"
     CDS             complement(5260..6459)
                     /locus_tag="WJL14_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ7656521.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHELHDRIQAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAACILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGLDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVGCGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            complement(6873..8042)
                     /gene="prpF"
                     /locus_tag="WJL14_001465"
     CDS             complement(6873..8042)
                     /gene="prpF"
                     /locus_tag="WJL14_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ7656522.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
BASE COUNT         1849 a         2400 c         2284 g         1819 t
ORIGIN      
        1 ttcagttgcg taggactggg tcgtcgaaag gggcggattc gatggattcg atgaaaacgg
       61 tagaaatgtg ggattgatgg aaatggcagg ctgaagccca tcctacagcc cgctgattcc
      121 ccggcaccga tgccgtctga accttcagac ggcatccaaa acccggtatt tcaaggcggg
      181 caggcggttg cggacgctgt tcaggcggtt ggcatcgatg tctgccgtaa cgatgccttc
      241 gccttcgggt aatacgtcca atacgtcgcc ccacggatcg acaatcatgc tgtgtccgaa
      301 cgtgcgccgt ccgttttcgt gcaaaccgcc ctgtgccgcc gccacgacgt aacattggtt
      361 ttcgacggca cgcgcgcgca gcagcagctc ccaatgcgcc ttgcccgtcg tgtgcgtaaa
      421 cgcagcgggc agcatcaata cgtcaaacgg cagctggcgg cggaaaaatt cgggaaagcg
      481 gacatcgtaa caaatgcccg ccgccaccga cacgccttct gccaacaagt gcggcacttc
      541 ccgccccgcg aggatggtat cggcttcggc atagcgttcg cccaaaccgg aaaagccgaa
      601 gaggtgcatt ttgtggtaca gccccgtccg tttgccgtcg cgcccgtaca ccaataaagt
      661 attcatgacc ttgcccgcct cggggctttg cagcggcacg gttccgccga acagcaccac
      721 gccgcattct ttcgccgttt cgctcaatgc cgtctgaaag cgtccgccgc ccaaaggctc
      781 ggcaagcgcg agtttgtcgg tatcgtttgc gcccatcagc acccaatatt cgggcagcag
      841 cacccaatcc acaccctgct ccgccgcccg tgcgaccagg cgtttcatgg cagcgacgtt
      901 ggtttccggc gacacgcccg acaccatctg cacggcggca actctgattt tgtccatttc
      961 ttctcctttt tcctgccgac cttgcggcat attccgccga taggctacca tgacggtact
     1021 ttgcgaaaac cgtttacaca aatgcccatt ttgaaccata ttgccctcat cggtgtaggg
     1081 ctgatcggcg gttcgttcgt tctcgacctc aaaaggcagg gactcgtccg caccgttacc
     1141 ggtatcgaca ccgaccgcga caacctcgaa cgtgcattgg aacgcggcgt gattgaccgg
     1201 gcttccgttg ccatcgacgc ggacagcatc ggcggtgcgg acttggtact gattgccacg
     1261 cccgtcgcca ccgttcccgc cattttgacc gcgctgcgcc ccgttttgcc ggaacacact
     1321 tggatttccg atgtcggcag caccaaatct tcggtcatcg aagccttccg ccgctgtctg
     1381 cccgaccgcc tgcaccgctg catcgccgcc cacccgattg ccggttcgga cagaagcggt
     1441 gcgcaagccg cgcagttcgg gctgttccgc cacagaaaac tcatcatcac gccacacggc
     1501 ggcgaacatt cagacggcat tgccttgata gaaaacctgt ggcacgcggt cggtgcggaa
     1561 atttatacga tggacgcgca acgccacgac gcggttttcg ccgccgtctc ccatatgccc
     1621 cacctgaccg ccttcgccta tgtccaccag attctcgacc accccgacgg acaggaatat
     1681 ctgaaattcg ccgccacggg ctttcgggac ttcacccgca tcgcctccgg tcatcccgcc
     1741 gtgtgggcgg acatctgcct tgccaacaaa gacagcctgc tgcaactggt tcaaggcttg
     1801 ggcaaacagt tggacgtttt ggcagacatc ctgaccgccg acgaccgcga agccctgtac
     1861 cgctattttg aagaagccaa aaccacacgc gaccgctggc tggacggcaa ctgaccgccc
     1921 tgccgtctga aaagcaaacc gcccagaccc gaaatctgcg gcggtttgtc tcaaccgggc
     1981 ctgccgtccg aacgcggacg aatcagccaa ggaatatact tccacgcata caccaaaagc
     2041 gcgagtgcaa acaaaaccga agaggtgcgt atgctgtgcg tgtaggcagt gccggaagaa
     2101 aatacggcaa ccatacggac ggcggttgcc gccatcatca gccaaaacgc aacgggaacg
     2161 gctttgggcg gcggataaat cggattgccc gtatgaccga gcgcggtacg cgccatcatg
     2221 cccaaagtca gcacgccgat accgccgacc ccgatcagat gcacacccag attgaggaaa
     2281 gcgggtttga aataagacgc gccgaccgca atcagcccca atccggtaaa cagatagccg
     2341 gcaaacagaa tccacagcat cggctctttc aacacaggct tataccacca gcggtacacc
     2401 tgcacggtaa aaatcacacc tgccgcaaac gcgaaagccg ccgacagcca aggcatcacg
     2461 ccgtgcgcca tcagcatggc ggtcagcatg ggcagccaca gcgaagcctg cgccacccat
     2521 ttcggactgg gaatctgcgg cacattcaac cgtttggacg taaaaaacga aataatccgc
     2581 gtaccaatca gaccgataaa acccgacacc atcaccaagc ccgactgcaa tccgctcaag
     2641 agtccgccta ggttgccgtt gtgcagctgg acgtggaacg ccgcgtgcgt accgcccaag
     2701 acgaacagcg cgaacacggc aacataattg cgttgattct gcgaacggat aacgggcaaa
     2761 gccatgcaca ccgcgccgta ccagaaaaac agcgtaccga gtatgccgct tgccgacgca
     2821 ccccaacccg ggataaaggc ggcaatccgc gcagccagcc aaaagatagt caagccgacc
     2881 agaacgccgc cccgcgtggg cggctgcccc gtccaagtgg cgacggcggt cagcaggaag
     2941 gcgatgacga ccagtccggc ataaccccaa atcatctcat gcgcgtgcca atagaaaccg
     3001 gacagctcgt gcgttcccgt gtagccgaaa ccccacagca atacggacaa tgcgccgtac
     3061 agagccgcca gtgaataaaa cgggcggaac gccattgccc aaacggggtg cttggtaaat
     3121 ttcatgtgtg ttccttgttt atcattaata tatttgccgc aaaacggaat catgccggca
     3181 aaaacggttc aacggcggga aacagctcgc gttcttcaaa gcgcgcgtga tcgcgcaggg
     3241 ttgtggcaaa agcggtattc cacgccgcgt taccgtattc ggggcttgcc atcatctgcc
     3301 gcagtcgggc gtggtctttc tcgaaacgtt gtttcaattc gggggcgaca ttctgccaaa
     3361 ttggggcaaa cttggcttct tcttcgcgaa aatgggtttc caattcggaa aaatgcggtt
     3421 cgagttcgtc ccgatgccgt tcttccggcg tccgcaacag acgcacgcac agggaaagcg
     3481 aatggtggtg gtcacgcgaa agcccgataa gggcgggatg tcgtttcaac ggtttcatga
     3541 ttttgagtta caataacaaa ttatcaaacg gtagcgcgga tgtttcagac ggcataccgc
     3601 cgcagacagg ctgcattcta ccgccccgac ggacagggtt tcaaccatta aggtattgtc
     3661 cgtgaacctt aaaccgtacc gataagaata acgatccata ccgagaaaat catgtatttg
     3721 acacaacata cggactacgg gctgcgcgtc cttatctaca ctgccatcaa cgacgatgcg
     3781 ctggtcaaca tcagtaccat cgccgtaacc tacggcattt ccaaaagcca tctgatgaaa
     3841 gtcgttaccg cgctggtcaa aggcggcttc ctccacagcg tacgcggaaa aggcggcggg
     3901 ctgaggctgg cctcgccgcc cgaacgcatc aatatcggcg cggtcgtccg ccatctcgaa
     3961 ccgatgcagc tggtcgagtg catgggcgag aacaacgaat gcctgattac accgtcctgc
     4021 cggctgacgg gcatactcgg cggcgcgatg aagtcgtttt tcacgtatct ggacggtttc
     4081 acgctccaag acctgctcaa caagccgacc tacgacctgc tctacgaacc gagaattccg
     4141 attgcggtgc agtaatgcat caaaatgccg tttcaaacgg atattccgac cgagcgcgat
     4201 acgcgccggc atttcggact gccgccggca aggcgcgcaa tgccgtctga acagccgatg
     4261 cttcagacgg catcggtttc cctacaaacc gaacaccgcc gtatcgccgc aaccttggcg
     4321 caccaattcc gtgccgtcgg tcatatcgac gacggtggtc ggctcggttc cgcaccagcc
     4381 gccgtcaatc accaaatcga cggcgtgttc caaacactcg cggatttcat aaggatcggt
     4441 caatggttcg ccgtcttcgg gcagcatcag ggtgcagctt aaaagcggtt cgcccaattc
     4501 ccccagcagg gcttgtgcaa tggcattatc gggaatacgc agcccgatgg ttttgcgttt
     4561 cgggtgcagc gtgcgcgccg gcacatcctt cgtcgcctgt aaaataaaag tataaggccc
     4621 gggtgtggcg gctttaagct gacgaaactg tacgttgtca attttggcgt acgtgcccaa
     4681 ctcgctcaaa tcggcgcaca tcagggtcag gtggtgtttc aaatcgattt tgcggatgga
     4741 gagtatgcgt tccatcgccg ccttatcgcc gagtttgcaa cccaaggcat aacaggaatc
     4801 ggtcggataa acgaccacgc cgcctttatt gacgatttcg accgcctgct tgatgaggcg
     4861 ttcttggggg ttgtcgggat gaatagcgaa aaactgtgcc atagtttgtg tccttatcgt
     4921 catgttttcc gtattgtacg cccgaaattc aaaatcccgt catttccaca aaaacagaaa
     4981 atcccgtcat tcccgcgcag gcgggaatcc ggttcgttcg gtttcggtca tttccaacaa
     5041 atccctgtcg ctttgcgttg ctggtttcct gctttcgtag aaatggcggc ggagggtttc
     5101 tgttttccga caaatattta tgtttacagg atgaaattcc accgtccgca caaaaatgca
     5161 agataacaaa aaaatgaaca atgccgtcta aaatcctcat cagcctaatc gggcgtttgg
     5221 ggtttcagac ggcattgtgc ggactcgtcc cttttttggc tacaagatgc cgacaagttc
     5281 ggcagtgtcg caggcaatca tcagttcttc attggtcggg acaaccaaaa cagccggaga
     5341 agaatcggtc gggctgataa tacccgaatt gccgtagcgt ttttccatat tggctttggt
     5401 gtcgatgtgc agacccaaga aatcaagata ggaaacggtt ttggcacgga tattacgcga
     5461 gttttcgccg ataccgccgg tgaacacgag tgcgtcaacg ccgccgcagc ccacagccat
     5521 cgaagcgatg tatttggcga ggcggtaggt cataacttcg agggcgaggc gcgcgccttc
     5581 gtggccttcg tcggcggcga tttcgagggt gcggcagtcg ttggagagtt cggaaatacc
     5641 gagcaagcct gattttttat tcagcatttc atcaacttgt gcaacatcca aaccggcgtg
     5701 tgaagtcaga tagctgtata cgcccgggtc gatatcgccg cagcgcgtac ccattaccaa
     5761 accttcgatc ggcgtgaaac ccatactggt atcgacggat ttgccgtttt tgatggcggt
     5821 aatggatgcg ccgttgccta agtgggcaat aatcatgcgg atgtcttcca gaggtttgcc
     5881 caagatgcat gcggcttcag gggcaacgta acgcatactg gtgccgtgga aaccgtagcg
     5941 gcggaaagcg tattttttac gcaactcgcg cggcacggca taagtgtagg cacgctccgg
     6001 catggtttgg tggaacgaag tatccatcac gccgacattg ggcagaccgg ggaaatgttc
     6061 ctgtgcggcg aggatgccgc tgatgttggc ggggttgtgc agcggcgcaa gcggaatgca
     6121 ggcattgagt tcgtccatta cggcctggtc gatcaaaaca gactcgctgt atttttcgcc
     6181 gccgtgggcg atgcggtggc cgacggcttg aatgcggtcg tgcagttcgt gtttttccag
     6241 ttcgttcaac agcataccca ccgcgccggc gtggcagttc cggccgctca ggggaacttg
     6301 gcgtttgttg ccgtctttgc tgaacgtaat gacggcttcg ggcgtggtca ggcgttcgcc
     6361 gaggcagctt aggacgacgc tgccgctttt gcggtcgata acggcacctt tgagcgatga
     6421 actgccgcag ttcagaacaa gaatgagttg gtcggacatg atgtttctcc ttgtagtttg
     6481 tggttgtttt ttggtgtggg gtcgtctgaa aaaggatggg cgttgggcgg agtgtgtttc
     6541 agacggcctc tttgggcaaa gcgtatgccg cattttctat cgttattccg tccccgccta
     6601 cgcgaggaca ggctgtggcg ggaatccaga atgtagaact cacgaaacct gttttcccct
     6661 accaatcctc catattgaca ggtctagatt cccgcctgcg cgggaatgac ggtaatacgg
     6721 gaagctgtct tttatcgggg tttccgtgtt cttccttgtg atttgccgcc ttccgccaca
     6781 gggcaaggga aaggaatggt cagtgatttg atgtttactt gtttttcaga cgacctgttt
     6841 atggctcgcg ggcaaaccca tgctacgtca atttaaaaac aatcttccgg caccctgacc
     6901 caaccttcca tcatcacgcg tgcgctgcgg ctcataaccg ctttggtggc cgtccattgt
     6961 ccgtcctgac attcggcggc tgcaccgacg cgcaatgtgc cggaaggatg cccgaagcgc
     7021 acttctttac gcgttccgcc gcctgcggca aggttgacca gcgtaccggg cacggcggcg
     7081 gcggtcgcaa tggcaacaga ggcggtaccc atcatcgcgt ggtgcaattt gcccatgctc
     7141 agggcgcgta ccagcaaatc gatgtcggcg gcattcacgg ttttgccact ggaggcggtg
     7201 taatcggcgg cgggcgcgac gaaggcgact ttcggcgtgt gcgcgcgggc ggcagcttcg
     7261 gatacgtcgc tgatcagacc cattttcagc gcaccgtaag cgcgaatttt ctcgaatttt
     7321 tccaaagctg cggcatcgtt gttgatgtcg tcttgcaact ctttgcccgt gtagcccaag
     7381 tcggcggcat tcaggaaaac ggtcggaatg cccgcgttga tgagcgtggc tttcaaacgg
     7441 cctatattcg gcacatcaat ttcatcgacc aaattgccgg ttgggaacat actgccttcg
     7501 ccgtcggctg gatcaagaaa ttcgatttgt acttcggctg ccgggaacgt tacgccgtcg
     7561 agctcaaaat cgcctgtttc caaaactgcg ccgttttgca tcggtacatg ggcaataatg
     7621 gttttgccga tgtttttctg ccagattttg actgtgcaga tgccgtctga aggaatcttg
     7681 cctttatcga ccaagccttg ctcgatggca aatgcgccca cggcggcggt gaggttgccg
     7741 cagttgccac tccaatcgac aaaaggtttg tcgatggaaa cttgcccgaa aaggtaatcg
     7801 acatcgtgat cggcgcgttc ggacttgtcc aaaatcaccg ccttgctggt ggacgagctg
     7861 gcgttgccca aaccgtctat ctgcttgccg tagggatccg ggctgccgag tacgcgcaag
     7921 aggattttgt cgcgtgcgct tcccgcttcc cgcgccgcct cgggcaggtc ggaacgtttg
     7981 aaaaacacgc cttttgatgt accgccacgg tagtaaacgg cgggaatttt aatttgcggc
     8041 atttctagat tctccttatg tagcgtgggc tctgcccacg atttttatag tggattaggc
     8101 tgccactccg accaaagccg tttgattccg caaactgtcg gggttcgccc caatctacgg
     8161 ctactgtgtt gtgatacggc agattattaa accccgtcat tcccgcgcag gcgggaatcc
     8221 agatttatcc gcacagaaac tcatcggata aaaaggtttc ctcaattcca ctttctggat
     8281 tcccgcctgc gcgggaatga cgatttacag tatagtggat taaatttata gtggattaac
     8341 aaaaaccagt ac
//
LOCUS       DAWKQB010000091         8225 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.091, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000091 DAWKQB010000000
VERSION     DAWKQB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.091"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WJL14_001466"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WJL14_001466"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ7656523.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1479..1715)
                     /gene="acpP"
                     /locus_tag="WJL14_001467"
     CDS             complement(1479..1715)
                     /gene="acpP"
                     /locus_tag="WJL14_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ7656524.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1947..2954
                     /locus_tag="WJL14_001468"
     CDS             1947..2954
                     /locus_tag="WJL14_001468"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ7656525.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTDNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     HQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVYGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGGDAADKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     R"
     gene            3287..3610
                     /locus_tag="WJL14_001469"
     CDS             3287..3610
                     /locus_tag="WJL14_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656526.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIALYKEDSYLVNMV
                     KRIFK"
     gene            3697..3978
                     /locus_tag="WJL14_001470"
     CDS             3697..3978
                     /locus_tag="WJL14_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656527.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4031..4504
                     /locus_tag="WJL14_001471"
     CDS             4031..4504
                     /locus_tag="WJL14_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ7656528.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4758..5093
                     /locus_tag="WJL14_001472"
     CDS             4758..5093
                     /locus_tag="WJL14_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656529.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            5268..7958
                     /gene="glnE"
                     /locus_tag="WJL14_001473"
     CDS             5268..7958
                     /gene="glnE"
                     /locus_tag="WJL14_001473"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ7656530.1"
                     /translation="MSDNRLDTARHHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRELRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYSAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQEMLKKLAELGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDQQTQTLPSSPEQQQLLAESMGFDSYAAFSDDL
                     NIHRNKVNQLFNEILSEPEEQTPDNSEWQWAWQDKPGEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAAQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKTCGGDTEAQMDTLRHFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAKKTEVTGELLAHYGNVRKLWREVFGEEAATA"
BASE COUNT         2052 a         2114 c         2155 g         1904 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcgtg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aaacccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tttggaattt
     1381 gcggcaggta tttaaacgga gtttaagcag tttgccatat aagggaaaag cctctattgc
     1441 gcggtgcagc agaggctgtt gtgtcgggcg acgaccggtt agccgttgtg ggcattgatg
     1501 tagtcgatag ccagttggac ggtggtgatt ttttcggcat cttcgtcggg gatttcgcag
     1561 ccgaatgctt cttccaaagc cataaccagc tccacggtgt ccaaagaatc cgcgcccaag
     1621 tcgtcttgga aagaagattc gtttttcacg tcggcttcgt ttacgcccag ttgttcagca
     1681 acaatttttt taacttgttg ttcgatgttt gacatatcag tcgttccttt atgccttgcg
     1741 gcaggttgtt taagggaaat aaatcggtgg tattgtaccg acttttaata gagttttcta
     1801 tctaatgact attatatcaa tatttgccga tttgtacatt tttgggtgcg gcgggttttg
     1861 tcgttcaagt ttgacctgtg tgccgtatgt ttggcgggat ttcggttaaa atggcggcat
     1921 ttccatctga agcagaaagc cctgtcatgt atccacttgc ccgtcgcatc ctgtttgcac
     1981 tcgatgccga aaaagcccac cacttcacgc tcgacgcgct ctacacggtt tataaattgg
     2041 gtttgattcc tgtaaccgac aaccgtacca aacctgtaaa attgatgggt atggatttgc
     2101 ccaaccctgt cggacttgcc gccggactcg acaaaaacgg cgaatacatc gacgcattgg
     2161 gcgcgctcgg ctttggtttc atcgaaatcg gcacggtaac gcccaaaccg cagcccggca
     2221 acccgcagcc gcgcctcttt cgcgttcccg aacaccaagg catcatcaac cgcatgggtt
     2281 tcaacaacca cggtatcgac accatgatac gcaacatcga aaaaagtaaa ttcagtggcg
     2341 tattgggcat caacatcggt aaaaacgcgg ttacacccat cgaaaacgct gccgatgatt
     2401 atttaatctg ccttgaaaaa gcctacgcac acgcaagcta cattaccgtc aatatttcct
     2461 cgcccaacac caaaaacctc cgcgcgctgc aaggtggcga cgagttgagc gcattgcttg
     2521 aggctttgaa aaacaaacag gcacagcttg cctctgtata cgggaaatac gttccgctcg
     2581 ccgtcaaaat cgcccccgat ttggatgaag cacaaatcga agacatcgcc cacgttgtca
     2641 aatccgtcga aatggacggc atcatcgcta ccaataccac catcgacaaa tcaagtctcg
     2701 gcagccatcc gctcgcaggc gagcagggcg gtttgagcgg gctgcccgtt catgaaaaaa
     2761 gtaatcgggt gttgaagctg ttggcagacc acatcgacgg caagctgccg attatcggcg
     2821 taggcggcat tatggaaggc ggggacgcgg cagataaaat ccgcttgggc gcgaccgccg
     2881 tccaagtgta cagcggattg atatacaaag gtccggcatt ggtcaaagaa tgtttgaagg
     2941 ctttggcgcg atgacgtgtc ccgcccaaaa tgccgtctaa acgcacgttt tgccgttcag
     3001 acggcatttt catttccttt ttccgcctgc cgccccttga aaatccctta cgcgccgccc
     3061 tgtttgaaac aaagcaaacg cccgccgcga accggacaat cttgatacgc agccaaagca
     3121 agtctggcgg taagttgtta aatatataag caattgatac aaaaaataat tttatactgt
     3181 ggcggacggc ttgggaaaac gcaaaatatg aacggcgttg aaattcatga tgtggaaaat
     3241 aaggatacgg cggataatca aaagatttta tatttaagga tttgatatgt ttaaggacga
     3301 actaaatgaa tttatccgat tgatatccga tccggaaagt gaattggatg aatggtattt
     3361 gagtgatttt aaagatgaac atatatggga aatgcaaagt tacgaagcat tttcatgttt
     3421 gcgggaagca gtcccctatt tgtttgctta tccccgatac gggtatgaac tgttggagat
     3481 aatttccgcg ttaaaagaaa catctgatac gacagaatta ttttatgagc cgggtatcgt
     3541 gcctttgctg attgctttat ataaagaaga tagttatctg gtaaatatgg tcaaacggat
     3601 attcaaatga tgaaaggggc gcgggcgttt gcttggcgga aggcaggtaa ctcaatttga
     3661 aggacgcgga agccgatacg ttaaaaagga tgaaaaatgg aaaacgggca acggaataac
     3721 cggtttcctt tggaaaaacg aattttttat ctggagcatt cggggcggta tctgatgatt
     3781 tgcgccttat cggattattc ccaaaacaaa catactgtcg ttatggcaaa tttcatctat
     3841 ccggatgaaa aaacggattg gcggaatttg gatgatttat tcaatgagtt ggttttagag
     3901 gaattgcagg cttcatttat ggattggcat ccgactgttg aagaggcaat cagccgtcat
     3961 ttggaagact tttcgtaaca ggcgtcaatc ggaaggggcg gaatgacggg gcaggcaatc
     4021 ggagtaaaaa atgaaccttg atttaaccgc gcaaaaagtc cgtctttctt ggaaggatat
     4081 tctgtggggg tatgggaata aatacttggg ttgggctgat gtggcagctt atgcccgaaa
     4141 aatgacgctt tcagatcatg atgaacgtgt gttcaaacta tctttaatca acaaatccaa
     4201 tattcttgaa ttaaagcctg ttctggaaga tttggcttcg gaaatgaggg attattcccc
     4261 taaaaattgg ctgtacgtcc tcttaagcga tgtattccat agaaaagaag aatttgagga
     4321 tcctttgggg gaagttgaaa aaatttatgc agattttgat tatccggaag aaatagaatc
     4381 atttgtcagg tatatgccgc ccaaagacgg ttatattcct tctgcccaca cctatgaaga
     4441 aaatattgcc cggttatatt ctcactggga acactatttg aacaacggcg gagggcaggg
     4501 ttaaaaccgg caatccgatg ccgtctgaag cattatccgg ccttcagacg gcaaacggat
     4561 tagagcggaa gatttgaaag cgggggcggg ctgcattccg gaagcggcaa aacccgaacc
     4621 gcccgcatcg ttgccgataa accgataagg aaatcgtttt gccggaccgg gtgcaaatgt
     4681 ttgataacaa tcctgccgcc cggcaaaaat gctttaaaga aagcaggatt ggttaattta
     4741 aaaggaaaaa ttaaaaaatg acggttgata aggaacaagt agaaaagatg atttattctg
     4801 aaaataaaca gagtgttatt gacggtatgc tcggtatgac cttcagctcg gaggaggatg
     4861 aaatcccctg gatttccgag aagttgacgg agttgtcgaa acataaagac ttggatattg
     4921 ccagattgtc attaacctgt ttcggacatc tcgccaggat gcatgaaaat atcggcgatt
     4981 gcgataaagt gattgcttta ttgctctcaa aacaaggtga tccggatttt caaggttttg
     5041 ccgaagatgc gttagacgag atttctttat ttatatttaa aaagcgtccg taagcgcgga
     5101 ttaaacctgt caatccgatg ccgtctgaag cgttatccgg ctttcagacg gcatcgaagg
     5161 ttgctatgat atagtggctt gactttaaac cggtacggct ccgccttgcc ctgatttgaa
     5221 gtgaatctgc catcatcttc ccgtcatcct tccaaacgga atccgaaatg tccgacaacc
     5281 gcctcgacac cgcccggcac cattccctgt tccttgcccg ccagctcgac aacggcaaac
     5341 tcaagcccga aattttcctg cccatgttgg acaaggtgtt gaccgaagct gatttccaag
     5401 cttttgctga ttgggacaaa atccgcgcgg aagaaaacga ggaagaattg gcgcggcagt
     5461 tgcgcgagtt gcgccgttat gtcgtgtcgc agattatcgt gcgcgatata aatcgcatca
     5521 gcgatttgaa cgaagtaacc cgtacgatta cgctgtttgc cgattttgcc gtcaataccg
     5581 cgctggattt cgcctacgcc tattatcggg acatgtacgg cacgccaatc gggcgttata
     5641 ccaaatcgcc gcagcattta agcgtggtgg cgatgggcaa ggcgggcggc tatgagttga
     5701 acgtgtcttc cgacatcgat ttgattttcg tctatcccga atcaggcgac accgacggca
     5761 ggcgcgaacg gggcaatcaa gagtttttca ccaaagtcgg gcagaaactg attgcgctgc
     5821 tgaacgacat caccgccgac ggacaggtgt tccgcgtcga tatgcggctg cggccggacg
     5881 gtgattcggg cgcgttggta ttgagcgaaa ccgcgctgga gcaatatttg attacgcagg
     5941 ggcgcgaatg ggaacgctac gcgtggtgca aaggccgcgt ggttacgccg tatccgaacg
     6001 gaatcaaatc gctggtgcgc cctttcgtgt tccgcaaata tttggattac agcgcgtatg
     6061 aggcgatgcg taagctgcac cgccaaatca gcagcgaagt cagcaaaaaa ggcatggcgg
     6121 acaacatcaa actcggcgca ggcggcatcc gcgaagtcga atttatcgcc cagatttttc
     6181 agatgatacg cggcggacaa atgcgcgcgc tgcaactgaa aggcacgcag gaaatgctga
     6241 aaaaacttgc cgagctgggc atcatgccgt ctgaaaacgt cgaaaccctg ctcgccgcct
     6301 accgcttcct gcgcgacgtc gaacaccgcc tgcaatactg ggacgaccag caaacccaaa
     6361 ccctaccctc ctcgcccgaa cagcaacaac tgctcgccga aagcatgggt ttcgacagct
     6421 acgccgcctt ttcagacgac ctcaacattc atcggaacaa ggtcaatcag ttgttcaacg
     6481 aaattttgag cgaacctgaa gagcaaacac ccgacaacag cgaatggcaa tgggcatggc
     6541 aggacaaacc cggcgaagaa gggcggcgat gccgtctgaa ggcgcacggg ttcgatgccg
     6601 aaaccgtcgc cgcaaggctc gaccaaatcc gccacggcca taaataccgc catctttccg
     6661 cacacgccca gccgcgtttc gatgcggttg tgccgctgtt cgtacaggcg gcggcagcgc
     6721 aaagcaaccc gaccgataca ttgatgcggc tgttggattt tctcgaaaac atcagccgcc
     6781 gatccgccta tctcgccttc ctcaacgaac atccgcaaac cttggcgcaa ctggcagaga
     6841 ttatgggcca aagctcgtgg gtggcggcgt atctgaacaa atatccgatt ctgctggacg
     6901 aactcatcag cgcgcaatta ttagatacgg cgtttgattg ggacaaactc gccgccgaac
     6961 tttcagacgg catcaaaacc tgcggcggcg acaccgaagc gcaaatggac accctgcgcc
     7021 atttccagca cgcccaagtc ttccgtctcg ccgtccaaga cctcgccgga ctgtggacgg
     7081 tagaatccct ctccgaccaa ctctccgccc tcgccgacac catcctcgcc gccgccctgc
     7141 tgtgcgcgtg ggcggacatg cccaaaaaac accgcgacac cccgcaattc gccgtcgttg
     7201 gttacggcaa actcggcggc aaagaactcg gctacgcctc cgacctcgac ctcgtctacc
     7261 tctacgacga tccccacccc gacgcaggcg acgtgtacag ccgcctcgcc cgccgcctga
     7321 ccaactggct ttccgccgcc actggcgcag gcagcctcta cgaaaccgac ctgcgcctgc
     7381 gccctaatgg cgacgccggt ttcctcgccc acagcatcgc cgcctttgaa aaataccagc
     7441 gcgaaaacgc ctggacgtgg gaacaccaat cccttacccg cgcccgcttc atctgcggca
     7501 cacccgaaat tcagacggcc ttcgaccgca tccgcaccga aatcctcacc gccgaacgcg
     7561 accaaaccgc cttgtcaggc gaaatcatcg aaatgcgcga aaaaatgttc cccacccacc
     7621 cgcctgccga cagtaacgtc aaatacgcgc gcggtggcgt ggtcgatgtc gaatttatcg
     7681 tccaatatct gatacttgcc catgcccgcc agtatccgca actcttggac aactacggca
     7741 acatcgccct cttaaacatc gccgccgact gcggtttgat tgacaaaacc ctcgccggac
     7801 aaagccgcac cgcctatcgc ttctaccgcc ggcagcaaca taacaccaaa ctgcgcgacg
     7861 ccaaaaaaac cgaagtaacc ggcgaactgt tggcacatta cggcaatgtc aggaaattgt
     7921 ggcgggaagt gttcggcgaa gaagcggcaa ccgcctgaac aaaaaatgcc gtctgaagcc
     7981 tgacaatctg ggtttcagac ggtattttcg taccgtgccg ttttaaggtt gcggcagagc
     8041 taaagcgatt tatcgggaat ggctgaaacc caaaaaccgg attcctcttt cgcgggaatg
     8101 acgggatttc aggcttctgt ttttgtggga atgatgggat ttgttaccca agcaaaaatc
     8161 aaaacaaaca aataagaacc gtttaaaacc ccgccgtttc cattaaaata gcgcattcta
     8221 ctttt
//
LOCUS       DAWKQB010000092         8050 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.092, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000092 DAWKQB010000000
VERSION     DAWKQB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.092"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(145..1941)
                     /locus_tag="WJL14_001474"
     CDS             complement(145..1941)
                     /locus_tag="WJL14_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ7656531.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGILSDMVSLTGKRTLAQSLAAKNIRIEHPDGLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2398..3651
                     /locus_tag="WJL14_001475"
     CDS             2398..3651
                     /locus_tag="WJL14_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ7656532.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3657..3809
                     /locus_tag="WJL14_001476"
     CDS             3657..3809
                     /locus_tag="WJL14_001476"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656533.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            3806..5317
                     /gene="lysS"
                     /locus_tag="WJL14_001477"
     CDS             3806..5317
                     /gene="lysS"
                     /locus_tag="WJL14_001477"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ7656534.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5501..5731)
                     /locus_tag="WJL14_001478"
     CDS             complement(5501..5731)
                     /locus_tag="WJL14_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ7656535.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLETEYSELNLNRYSVGSP"
     gene            complement(5735..6613)
                     /locus_tag="WJL14_001479"
     CDS             complement(5735..6613)
                     /locus_tag="WJL14_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ7656536.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIILNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            complement(6643..7770)
                     /locus_tag="WJL14_001480"
     CDS             complement(6643..7770)
                     /locus_tag="WJL14_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7656537.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
BASE COUNT         1769 a         2034 c         2245 g         2002 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atttgcttgc
       61 ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa gggctttcag
      121 acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt tgatcagcca
      181 cgcttttgcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg cgtggtagcg
      241 gttgacccag tcgatttcgc cgtcggtcat gagggcggtg tccatcaggc gggtgtcgat
      301 ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt cttgaggggc
      361 ggcgacggct tggttggcgg caaggttttc aatgcggatg ccccattttc ccgggcggta
      421 gagtccgggt tcgatggagg taaccatgcc ttttttcatg gcggtttcgg gcgtggcggg
      481 ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc ctacgccgtg
      541 tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc aaatcgcatc
      601 aatcagcggc gaggggatgt tttcggggaa cacggcttcg gcaagcgcga tatgggcttt
      661 gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag ggacgacgcg
      721 ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagca aaagcccgtt
      781 gccgctgatg gtgctgtggc tttcgggcgt tgcgctgtaa tgcggcagtg cgccgttggc
      841 gttgaagcct gcgatggtgt cgaaactcaa tgaaatgaag cctgggcgca cgctgcggtg
      901 gcgataaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc cgatgatgtc
      961 ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg cttcgcggat
     1021 gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt tgattccctc
     1081 gataaggcgc acgctttcgg gcaggcgcac aagcgtgctg acggcggttt tgttcggttc
     1141 gataagcaac gcgccgccga tttgcgcgag tttgtcggca acttgggcgt aaggctcgac
     1201 cgtgatgcct gcggtttgca gcgcggcggc ggcttcggca ttcagacggc atcggtcggt
     1261 aaacaggacg gcgttgtctt tgccaatcaa tagataggac acgaaaacgg gattgaaagg
     1321 cacgtcgctg ccgcgcaggt tggtcagcca ggcgatgtcg tcgagcgagg aaaccaagtg
     1381 gtaatccgcg cctttttccg ccatcacggc gcgcacgcgg gcgagttttt cggcggcggt
     1441 ttcggagacg taggcggggt cgtggacgaa cacggtttcg gcaggaatgg ccgggcggct
     1501 tgtccacact tggtcaagca ggccgtcggg gtgttcgatg cggatgtttt tggcggcgag
     1561 cgattgcgct aaagtgcgtt tgccggtgag cgagaccata tcggaaagga tgccgacggc
     1621 ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt gcccgctttt
     1681 ttgcagttcg atgccgctgc ccgcaagctg cttggcggct tgttcccaat agcggctgtc
     1741 cacccatacg cccgcttcgt cggcggtaac gacgaatgtg ccgaccgagc cggtaaagcc
     1801 cgacaattcg cgccgcgcct gccaatgttc gggcaggtat tcggacaggt gggggtcggc
     1861 ggaagggatg acgagtgcat ccaagccttg cgccttcatg gcttcgcgca atgcggacag
     1921 gtaattcgat acggtattca taacagttcc tccaatcggg ttttgcggct tcagacggca
     1981 tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat cgccagcctg
     2041 ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaaagc gatgtaaggt
     2101 tgtttgttgt aaaattttag gcataagata tcgttttatg cctttttttg gaagtaatat
     2161 ggaaaatata gtcaagtttg agcatgaagt tctaatagtg tgtgttggca ggtatttgga
     2221 ttacgttaca atacgcgctt tcatcaaaat ttccctaccg agcggtatcc tgtttttgaa
     2281 gacggtagga tttgggtttg tttgggggaa tcccgcccaa attggagcaa tagatagggc
     2341 tgtgtccgaa tatgcggctc tgtgtgttga aacacggtta aaaatacgga agactttatg
     2401 tccgaacaac atatttcgac ttggaaaagt aaaatcaacg cattgggacc ggggattatg
     2461 atggcttcgg cggcggtcgg cggttcgcac ctgattgcct cgacgcaggc gggcgcgctt
     2521 tacggctggc agatcgcgct catcatcatc ctgaccaacc tcttcaaata cccgtttttc
     2581 cgcttcagcg cgcattacac gctggacacg ggcaagagcc tgattgaagg ttatgccgag
     2641 aaaagccgcg tttatttgtg ggtattcctg attttgtgca tcctctccgc cacgattaac
     2701 gcgggcgcgg tcgccattgt aaccgccgcc atcgtcaaaa tggcgattcc ctcgctgatg
     2761 tttgatgccg gcacggttgc cgccttgatt atggcatcct gcctgattat tttggtgagc
     2821 ggacgttacc gcgctttgga tcgcgtttcc aaaatcatca tcgttacttt gagtatcgcc
     2881 acgcttgccg ccgccggcat cgctatgtcg cgcggtatgc agatgcagtc cgattttatc
     2941 gagccgacac cgtggacgct tgccggtttg ggcttcctga tcgcgctgat gggctggatg
     3001 cccgcgccga ttgaaatttc cgccatcaat tctttgtggg taaccgaaaa acaacgcatc
     3061 aatccttccg aataccgcga cgggattttt gatttcaacg tcggttatat cgccagtgcg
     3121 gttttggctt tggttttcct tgcactgggc gcgtttgtgc aatacggcaa cggcgaagca
     3181 gtgcagatgg cgggcggcaa atatatcggg caattgatca atatgtacgc cgttaccatc
     3241 ggcggctggt cgcgcccgct ggtggcgttt atcgcgtttg cctgtatgta cggcacgacg
     3301 attaccgttg tggacggcta tgcccgtgcc attgccgaac ccgtgcgcct gctgcgcgga
     3361 aaagacaaaa cgggcaacgc cgaattcttt gcctggaata tttgggtggc gggcagcggt
     3421 ttggcggtga ttttctggtt tgacggcgta atggcgaatc tgctcaaatt tgcgatgatt
     3481 gccgcttttg tgtccgcccc tgtgtttgcc tggctgaatt accgtttggt caaaggtgat
     3541 gaaaaacaca aactcacatc aggtatgaat gcccttgcat tggcaggctt gatttatctg
     3601 accggtttta ccgttttgtt cttattgaat ttggcgggaa tgttcaaatg ataacgatgc
     3661 cgtctgaaac cgcaaaccgc tttcagacgg cattgccgcg tttatggaaa aaaatgccga
     3721 catcgcgtaa aatacgcgca aattttgtga ttcggtcagg tcgtctgaaa cagattgcgc
     3781 ctgatttatt ttttcggaaa accctatgag cgaacaaaac catccgcaaa ccgagccgca
     3841 gttggacgaa aaccaaatca tcgccctgcg ccgcgaaaaa ctgcacaaca tccgccaaca
     3901 gcgcaacgcc tatcccaacg acttcaaacg cgacagcttc gccgccgatt tgcacgccca
     3961 atacggcgaa atcggcaaag aagaactcga tccgcaaggc attcccgtca aagtggccgg
     4021 ccgcatgatg ctgaagcgtc aaatgggcaa ggcgagcttt gccaccattc aagacgtgtc
     4081 cgggcaaatc cagctttatc tgaacaacaa aggcgtgagc caagaagttt tggacgactt
     4141 caaccattgg gatttgggcg acatcgtcgg cgcggaaggc actttgttca aaaccaacca
     4201 cggcgaactg accgtacgcg tgtccggcat ccgcctgctg tccaaatccc tgcgccctct
     4261 gccggacaaa cataaaggct tgagcgatca ggaaaccaaa taccgtcaac gctacgtcga
     4321 tttgattgcc aacgaagaat cgcgcaatac cttcatcaaa cgcagccaaa tcatccaatc
     4381 cgtgcgtaat tttatggtgg gcgagcatta tctcgaagtc gaaaccccga tgatgcaccc
     4441 gattcccggc ggcgcgacgg caaaaccctt cgttacccat cacaatgcct tagatattcc
     4501 gctttatttg cgaatcgccc ccgagctgta tctgaaacgc ctggttgtcg gtggtttgga
     4561 acgcgtgttt gaaatcaacc gcagcttccg caacgaaggc atgtccgtgc gccacaaccc
     4621 cgaattcacc atgatcgaat tctacgaagc cttctccgac tacgaacgca tgatgcagat
     4681 ggcggaagac atcatccgca acgcatcgca cacggtaaac ggcacggcaa acatcactta
     4741 caacggcaaa gaagtcgatt tggaaagccc gtttgaacgc ctgaccattc tcgaagccat
     4801 caaaaaatac aatccgcact acaccgacga gcagttgaac gatgcggaat ggctgaaaaa
     4861 agaaatcgtc aaacacggcg aaagcctgcc gccgtccccg ggcatcggca gcctgcaact
     4921 cgcgctgttt gaaggttgcg ccgagggcaa gctgtggaat ccgaccttca tcgtcgatta
     4981 cccggtcgaa gtttccccgt tggcgcgcgc ttcggatacc aaacaaggtc tgaccgaacg
     5041 tttcgaattg ttcgttgtcg ggcgcgaact ggcaaacggc tattccgagt tgaacgaccc
     5101 cgaagaccaa gccgaacgct tcaaagcgca agtggcgcaa aaagacgcgg gcgacgacga
     5161 agcgatgcac tacgatgccg actacatccg cgcgatggag ttcggtctgc cgccgaccgg
     5221 cggttgcggt atcggcatcg accgcttggt aatgttgctg accgattcgc aaaccatccg
     5281 tgacgtgatt ctgttcccgc agatgcgccc cgagtaatca taaaaacagt tgaaatggca
     5341 atgccgtctg aacccgattg gattcagacg gcattttgta tggcaggatt gatagtgaat
     5401 taacaaaaat caggacaagg cggcgagccg cagacggtac aaatagtacg gaaccaattc
     5461 acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag ccaacgctgt
     5521 accggtttaa atttaattca ctatattcgg tttccaactg accgacccat tctgacaagg
     5581 ccgacaggcg ttgttcggat ttcgccacga acgggttttc ggtatcccac gcgtagcctg
     5641 ccaaaatcga agaaagcata cggctgattt ccgggctgcg gtcgggcgcg tacacgacgt
     5701 tttggaagcg gaaatcgtac caaccgtcca cacatcaggg gcggtaatcc gaatcaacac
     5761 gccttcttgc ctgtgcggcg gacataaacc gttttttgac ctccagcacc acacctaaaa
     5821 tcacgctgcc ttcgggcagg tcgagggctt cagacggcct gcccgtctga taaatcagca
     5881 tcctgcccgc aatatcgata acaaggtgcg cgacgaccag ccgcttgtcg gacagttcaa
     5941 tcagtacgat gtcttcgtcc gcagggcgca tacgcggctc gaccaccagc aaatccccct
     6001 tttcgataac cggcgacatg gacgtgtcgg gcatggcaac ggcaaacgta tattgtgaag
     6061 gggcgaccac cgaagcaaac aggtcgaaac tgcccgtgag tttgcctgta cgggcgaaag
     6121 ccgccgcctg acggtctgac aacaaaggac ggggtgtgag cctcatcgac agtgctttgc
     6181 cgtcggcatc gctgtggttg tgcgtatgcg tatgatggtt gttgatggtc gtatcgcctt
     6241 ggacatcgtt aaggattatg ctgtttgaac ctgccacgga tttggtcatc tcattgtagg
     6301 cttgcgtcgt gatgcgctct tgcttggtca tacctgttac agcctgtttc agcatttcta
     6361 acgctatgtg attcttccta agggtcgcta taccggcttt tgcagcttgc tcattaccac
     6421 aagaggggca accccagcta cttcctatga acatattgga gtacctcgaa caggtttggt
     6481 ttccatgttt agggcagttg attacacacg gcttggctgt accctcaaac tcgaccaact
     6541 taatgccacg atgaccgaaa cgttcctcaa cctcagcagc agcttcttca aatgtttttg
     6601 accttcttgc catattgacc tccggattat tgattatttt ggctatgctt taacgaggga
     6661 ttggaatgag gtaaagctgt tgccctctcc ccgcaatagt tccattaggc gcggatgaat
     6721 gaatagtttg tccctgccaa tggcgatttc ttgcagcaca cctatgtctg aaagctcttt
     6781 caggtactta gaggccgtct gccgtttggc tatccctgcc gcttctaggt tggcaatgcg
     6841 tgtatatggc tgctcaaaca gaagatttac cagttcgtgc gtgtagattc cttgtgcgtg
     6901 tgtccgtatg tgttgacgtg tctgctcgaa caggcggcgt atcgcatcta ttttcgatac
     6961 cgtccaatcg gcggtgtcgg ctacgccgtc taagatgtag attatccagc tttcccaatc
     7021 ctgccgttcg gttacgccta aaagcaggcg gtaatagtcc gccctgtttt cgatgatgta
     7081 gcggctcaaa tacaaaatag gcaaatccaa aagccctttt tcaatcaata gcaggctgtt
     7141 caatatgcgc cccgtccgcc cgttgccgtc cgtaaacgga tggatggctt caaattggta
     7201 atgtgccgcc gccatgatga taagcgggtc taaatcgccg ctttcgtgaa taaaccgctc
     7261 ccaatttgcc agcttgccgc gtatggtttc ttctccttcg ggcggggtat agacaacatt
     7321 tccgctgttg cctcctttta gggctgtgcc gcctgttttg cggatggcca tttcgtaggg
     7381 gtgcttgatg gcgttgcaga ccatgatggc ggtttgtgtg cataaagggc ggctcgtcag
     7441 tgattcatag cctgcaaaca gggcggtgcg gtattgcagg gcttctttcg tggcagggtc
     7501 ttgccgttcc gtatccattt gcagggattg aaacagctta tccgttgtgg ttacgatgtt
     7561 ttcaatttcc gaacttgcac gggcttccat aacaggaagg gtgttaatca gcatggcttg
     7621 attcggtatc agttctgccg cctgctttaa acgggcaagg gatgcacggg cggctataca
     7681 acgtttcagg atggttttgc tttcaatatc ctgttttggc ggcaggggtg gtaaatcgtt
     7741 atagggaata tcgggtttcc agttgctcat atttaaaatt tcaaaaaatt taaagatgtt
     7801 tccagtatat gtttacgccg tgtatatatc aaggatatat gtttaaaaat ttggcttttg
     7861 taaatatatg ggcggtaaaa ccgcattatt ttatgctgtt acctatttta caccttgtct
     7921 gcacacgatt tccaaacaac ctaaaaagaa acctgccgaa agacaggttt aaagaggtca
     7981 ttttaaatgt gaactaaagc ttacaccctc gtagggtatc gtgtatatca gacgtactta
     8041 atatacacta
//
LOCUS       DAWKQB010000093         7886 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.093, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000093 DAWKQB010000000
VERSION     DAWKQB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.093"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            296..1609
                     /locus_tag="WJL14_001481"
     CDS             296..1609
                     /locus_tag="WJL14_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ7656538.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1827..2002
                     /locus_tag="WJL14_001482"
                     /pseudo
     CDS             1827..2002
                     /locus_tag="WJL14_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            1998..3248
                     /locus_tag="WJL14_001483"
     CDS             1998..3248
                     /locus_tag="WJL14_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ7656539.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRIFVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3283..4776)
                     /gene="rng"
                     /locus_tag="WJL14_001484"
     CDS             complement(3283..4776)
                     /gene="rng"
                     /locus_tag="WJL14_001484"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ7656540.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            4990..5247
                     /gene="grxC"
                     /locus_tag="WJL14_001485"
     CDS             4990..5247
                     /gene="grxC"
                     /locus_tag="WJL14_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ7656541.1"
                     /translation="MQTVTMYTGPFCPYCTMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5268..5711
                     /gene="secB"
                     /locus_tag="WJL14_001486"
     CDS             5268..5711
                     /gene="secB"
                     /locus_tag="WJL14_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ7656542.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5797..7839
                     /gene="recG"
                     /locus_tag="WJL14_001487"
     CDS             5797..7839
                     /gene="recG"
                     /locus_tag="WJL14_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ7656543.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAIGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1650 a         2059 c         2333 g         1844 t
ORIGIN      
        1 tcccgcgtgt acggcacgcc ccgcgctccg caatgatagg ggcggtttcg tttgaatcgg
       61 cgcaccggga tgaatgcccc cgcgccgtcc cgataccgat ttgccggttg ttccgtatcg
      121 gttgccgccg tgaaatgacg gacggatgca ggaatggcgg caggcgtgaa tgggaggaaa
      181 tgaccgattg acttgtttca aaataaaaaa gtagaatgca gggcttatcc cgcgtctgtg
      241 ggcggttttg cctgcacggt cggttttctg tttaaaataa aataaaggag aaatgatgct
      301 gacgtttatc ggactgctga ttatcggggt catcgtatgg ttgctgctga cggaaaaagt
      361 gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg gcgttgctgg cggggtttga
      421 tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa tcggtgatgc agattgtgat
      481 tatgtttatg ttttccattt tgttttttgg aatcatgaac gatgtggggc tgtttcgtcc
      541 gatgataggc ggtttgatta agctgactcg gggtaatatc gtggcagtga gtgtggggac
      601 ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt gcgacgacgt ttttattggt
      661 cgtccccgcc cttttgccgc tttacaagcg tctgcatatg aatccttacc tgttgttttt
      721 gctgctaact tccagtgcgg gattgattaa ccttctgccg tggggcgggc cgactgggcg
      781 ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat aaacctttgt tgacggtgca
      841 aattatcggt gtagtgttta tccttgcgct gtccctgctt ttgggtgtgc gtgaaaaaag
      901 gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg gatttgataa agccggtgcc
      961 tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg ttttggtgga atgtcctgct
     1021 gtttttggcg gcgatgagcc tgcttttttc gggcatcttc ccgccgggtt atgtatttat
     1081 gctggctgca acggcggcgt tgcttttgaa ttaccgcagc ccgcaggaac agatggagcg
     1141 gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc attattttgg cggcaggtac
     1201 gtttttgggg attttgaagg gtgcggggat gttggacgcg atttccaaag acattgtgca
     1261 tatcctgccg gacgcgctgc tgccttatct gcatattgcc atcggtgtgt tgggcattcc
     1321 gcttgagttg gttttgagta cggacgctta ttatttcgga ctgtttccga ttgtggagca
     1381 gattacctcg caggcgggcg tggcgcccga agcagcaggt tatgcgatgt tgatcggcag
     1441 tatcgtcggc acttttgtta cgccgctttc gccggctttg tggatgggct tgggtttggc
     1501 gaaattgtcg atgggcaaac acatccgtta ttcgtttttc tgggcgtggg gtttgtcgct
     1561 ggcgatattg gccagttcga tagcggcagg aatcgtgcct ctgccgtaaa cggcgaagcc
     1621 gtctgaagcc tgaatcgttc ctgtggtctg tcgcaggctc tgcctgcccg tgtgttgcaa
     1681 tcgcgcccca atgccgtctg aaggcagttg ccggcgcggt tttttgtgcc gcagttttga
     1741 aaaataccgg caagcgtaaa ggccgctaaa ttgcggctaa ttttggggtg ttagaatagg
     1801 cacggtttat tttgaaggga aagttgatgc gtgttttgct ggtggaagac gatgcgatga
     1861 ttgcggaggc ggtgtcggca agtttgaaag acggcggcta tgcggtggat tgggtcaaaa
     1921 acggcgcgcg gctgccgctg cccagcctta tgacttgatg ctgctggatt tgggtttgct
     1981 ggggcgggac ggtttggatg tttgttccgc tggcaatgct tgcaggtatg ttttcctact
     2041 acgaaacctt ccacgaaacg gaagcgttgc aggacgacct gctccgtcag gcggcattgt
     2101 acgtcgcacc cgattccaaa cccgaaactt tgcccgaggg cgacggcgat acgcgcattt
     2161 tcgtgcagat gccgcagcag gaagaccctg ttgtcagcct gcctgcgcat ctggcggacg
     2221 gtctgcacac gcttcaggcg gacggggacg acgattatta ccgcgtctat atccgcacga
     2281 ccgaacaggg acggattgcc gttatgcagg aaaacgaata ccgtgaagat ttggcggcgg
     2341 atgcggcacg gcaaagcgtg ttgcccctgc tggcggcact gccgctgatg atactgctga
     2401 ccgtgtggat tacgcacaaa gccatgcgcc ccgtccgcaa attgtcgcaa agtctcgaac
     2461 aacgccgaat caatgacctg tctgctttga gtgtggacaa tattcccagt gaaatcagag
     2521 ggttcgtaac cgccatcaac ctgcttttga aacgtgcgga tgaagacatc cgccaccgtc
     2581 agcggtttgt cgccgacgcg gcacacgaat tgcgtacgcc gatgacagcc ctttcccttc
     2641 aggcggaacg gctcaacaat atgtcgctcc cacccgatgc agcgcggcag cctgccgttt
     2701 tgcagcagag catcaggcgc aacaaacacc tgctcgaaca gcttttggca ctggcgcgtt
     2761 cgcagtcgga tgaaacccct ctgacgaaaa cgacattcgg tctgcaaagc cgtttccgcc
     2821 aagtgttgca ggaactgatg ccgctggctt tggaaaaacg tcaggacatc ggcgtggcgg
     2881 tcggaggcga tgtcgaagtg tctgccgacg aaacggaaat ctatacgctg attaaaacct
     2941 ttgccgacaa cgcggtacgt tacacgccgc ccgaaggcag gatcgatttg ggtttcacgg
     3001 acgaagggaa atatctcgcc gtgtgggtgg aagataacgg gaacggcatt cccgaatccg
     3061 aacgcgcccg cgtcctcgat ccgttttacc gtattttggg aacggagcag caggggacgg
     3121 ggctgggtct gtccatcgcc gacacgcttg ccaaaaaata cggcggacat ttggaactga
     3181 ccgacagccg acgtttcgga cacggtctgt tgatacgggc gttgttggac aaggaaaccc
     3241 tgaaatagac agatgccgtc tgaaggcttc agacggcatt ttttacatca gcactatatc
     3301 gtattgttcc tgcgtgtaag cggtttcgac tgccaaagaa atcggtttgc cgatgaaatc
     3361 tatcagcatt gccaaggatt gcgattcttc gtccaaaaac aaatcgatga cgttgggggc
     3421 ggcgaggatg cggaaacttt cggcatcgta acggcgcgct tcgcggacga tttcgcgctg
     3481 gatttcgtag catacggttt gcggcgtttt cagacggcct ctgccttggc aggaagggca
     3541 gggttcgcag aggacttggt ttaagttttc gcgcgagcgt ttgcgcgtca gctcgaccag
     3601 tcccaggctg gtaaaaccgt gcagggtaac gcgggtacgg tcgaaggcga gggctttggc
     3661 aagctcctgc aacacggctt cgcggtggct ttcctgcgcc atatcgatga agtcgatgat
     3721 gatgatgccg ccgaggttgc gcagcctcaa ttcgcgggcg atggtgtggc aggcttcgag
     3781 gttggtgcgg aaaatcgttt catcgaaatt gcgtgcgccg acgaagccgc cggtgttcac
     3841 gtctatcgtg gtcatcgctt cggtggattc gataatcagg tagctgccga agttgaggtt
     3901 gacgcgcggt tgcagggcgc ggctgatttc ctgctcgacg ttgtgggttt caaatagcgg
     3961 gcgttcgcct ttgaacaatt ctatcctgcc caatgcgccg tggacgtatt gttcggcaaa
     4021 acgcgtcatg cggccgtggt ttacggtgga atcgacgagg attttttgcg tgtcgcagcc
     4081 gaccatatcg cgcaacacgc gcaaactcaa gggcaaatcc tgataaagca gggtttccgg
     4141 cggccggatt ttcgcctgtt cttggatgtg ttcccacact ttggtcaggt agtcgatgtc
     4201 ggactggagc tgttcgtcgg tggcgttttc ggcgttggtg cggatgatgt agccccggca
     4261 ggcattttcc ggcaggagct tgtcgaggcg ttcgcgcagg ctgctgcgtt cggcatcgtc
     4321 ttcgatgcgt tgggacacgc cgatgtggtc ttcttgcgga agatggacga ggaaacgccc
     4381 cgccagcgag atttgggtgg aaagccgcgc gcctttggtg ttgatcgggt ctttgatgac
     4441 ctgcaccaaa acagactgcc cttcaaacag catatgttcg atgcgctggg tttcttcggg
     4501 gttgcggcgt tgttcgagga catcgacgat gtgtaaaaac gccgcgcgtt ccaagccgat
     4561 gtcgataaac gcgctctgca tcccaggcag cacgcggcgc accacgccca aatagatatt
     4621 gccgactagg ctgtgttcgc tgttgcgctc gatgtgcagc tcgcagatat tgttttcctc
     4681 caacaccgct acgcgcgttt cctgcggcgt gatgttgacc aatatcgttt cgggcgggcg
     4741 cgcgatgtct ttggggatgg ggagtcctga caacatggtt tttcctgaaa atgtaataaa
     4801 aatatttttg catccgttcc gccgcgcagc gcgggtgcaa aaatattttt cacaatgtct
     4861 atcatacttt aaaaaagaaa ctttgacaca ttccgcacat gccgtttgaa cgtgcatctg
     4921 ttccgcactt gccaaacggg ggaaatgccc ctatattgat tatcattgca aaactttcgg
     4981 aaacccaata tgcagaccgt taccatgtac acaggtccgt tttgccccta ctgcacgatg
     5041 gcgaaaaggc tgctgcacgc ggcaggtgtc ggacatatcg acgaaatccg tgtcgatgca
     5101 agtcccgaag cctttgccga aatgcagcag ctttccggac agcgcagcgt gccgcagatt
     5161 ttcatcggcg aaacgcacgt cggcggattt accgacctct accgcctcca gcaggaaggc
     5221 gggctggacg gactgctgaa cccttaaccc ccaactagga aaacaaaatg agcgaagaac
     5281 tgcaacccgt attcagcatc gagcgactgt atgtcaaaga cttgtctttg gaagtgccgc
     5341 acgcgccgca aatctttttg gaacagggcg agcccgaagt ggatatgcgc gtttccaccg
     5401 gcagccaaaa gctggaagac ggctactaca acgtggatgt aaccgttacc gtaaccgcca
     5461 aattggataa cgagcgcacg atgtttttga acgaagtaac ccaaagcggt attttccgtt
     5521 tggaaaacat ccccgaagaa gatgccgacc tgctgctcgg cgtggcgtgt ccgaacatcc
     5581 tcttccctta cgcgcgcgaa gccgtttccg gtacggtaac gcgcgccggc ttcccgcccg
     5641 tcctgctcgc gccgattaat tttgaggcga tttaccaaca acagcaggaa gccgaagccg
     5701 ccggagcttg attcctgcct gtcgatgccg tctgaatccg tttcagacgg catttttgtt
     5761 ttcggataaa ataggcgcgt ccaaaggaat ctgcctatga tgtcgcccga aacccgaaaa
     5821 cagctcaaaa tcaccgatgt ttccgccaag aagctcgaca aactcaacct ccataccgcg
     5881 tgggatttgg tgttgcacct gccgctgcgt tacgaggacg agacgcacat tatgccgatt
     5941 aaggacgcgc cgattggcgt gccgtgtcag gtcgaggggg aggttatcca tcaggaagta
     6001 acgttcaaac cgcgcaagca gctgattgtc caaatcgccg acggctccgg cagcgtcctt
     6061 tttctgcgct tcatccactt ttacgccagc catcagaaac agacggcgat cggcaaacgc
     6121 atccgcgccg tgggcgaaat caaacacggc ttttacggcg acgagatgat tcatcccaaa
     6181 atccgcgatg ccgagggcgg cggtttggcg gaaagcctca cgccggttta cccgaccgta
     6241 aacggtttga accagcccac tttgcgccgt attattcaga cggcgttgga cgttacgccg
     6301 ctgcacgaca cgctgcccga tgccctgctg tgccgtctga agctgccaca cctcgccgaa
     6361 agcctgcgtc ttttgcattc gccgccgccg agtttcacga ttcatcagct ttcagacggc
     6421 acgctgcctg catggcaacg gctcaaattc gacgaacttt tggcgcaaca gctttcgatg
     6481 cgcttggcgc gacagaagcg tatcggcggc acggcggcgg cattgggcgg cgacggcaca
     6541 ttgacccaag ccctgcgcca agccctgccg tttgccctga ccgatgcgca agaaaaagtt
     6601 gtttccgaaa tctgccgcga tatggcgcaa acctacccga tgcaccgcct gctgcagggc
     6661 gatgtcggca gcggcaaaac cattgtggct gctttgtctg ctttgacggc tatcgaatcc
     6721 ggtgcgcagg tggctgtaat ggcgcccact gaaatccttg ccgaacagca ttttattaaa
     6781 tttaaacaat ggctcgaacc tttgggcatt gaagttgtcc gcctttttgg cagtttgcgt
     6841 aaaaaagcca aagacgaagc caaagccaaa ctggctgacg gcagcgtcaa aatcgccgtc
     6901 ggcacgcacg ccctgttttc agacggcgtg gcgtttcaca atttgggctt gaccattgtg
     6961 gacgaacagc accgtttcgg cgttgcccaa cgcctcgccc tcaaaaacaa agggcgcgaa
     7021 gtccatcagc tgatgatgtc cgccacgccc atcccgcgca cgcttgcgat gagttttttc
     7081 gccgatttgg acgtgtccgt catcgacgaa ttgccgcccg ggcgcacacc gattaaaacg
     7141 cgcctcgtca acaacgtccg ccgcgccgaa gtcgaaggct tcgtcctcgg cacttgccga
     7201 aaagggcggc aggcgtattg ggtctgccca ttgattgaag aaagcgaaac cctgcaactg
     7261 caaaccgccg ccgaaaccct cgcccggctt cagacggcat tgcccgaact caatatcgga
     7321 ctggtacacg ggcgcatgaa ggccgccgaa aaggccgaag tgatggcgcg gttttcttcg
     7381 ggggggctga acgtcttggt cgccaccacc gtcatcgaag tcggcgtaga tgtgcccaat
     7441 gccgccctga tggtcatcga acacgccgag cgcatgggct tggcgcagct tcaccaatta
     7501 cgcggacggg tagggcgcgg cgcggcggaa agcgtgtgcg tcctcctgtt tgccgaaccc
     7561 ttgggcgaac tcgccaaagc gcggctgaaa gtcatctacg aacacaccga cggcttcgaa
     7621 atcgcccgcc aagacctcaa tatccgcggc ccgggcgagt ttctcggcgc gcgccaaagc
     7681 ggcgtgccta tgctgcgttt cgccaaactc gaagaagacc tgcacctttt ggaacaggcg
     7741 cgcgaaaccg ccccgatgct gattgagcaa aaccccgaaa tcgtcgaagc gcatttggca
     7801 aggtggcttt ccggcaggga agggtatttg ggcgtgtgag caaaatgccg tctgaaatat
     7861 tagaaaaaaa agtgtaacgg aaatct
//
LOCUS       DAWKQB010000094         7796 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.094, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000094 DAWKQB010000000
VERSION     DAWKQB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7796
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.094"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..1337)
                     /locus_tag="WJL14_001488"
     CDS             complement(<1..1337)
                     /locus_tag="WJL14_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ7656544.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSG"
     gene            complement(1399..2748)
                     /locus_tag="WJL14_001489"
     CDS             complement(1399..2748)
                     /locus_tag="WJL14_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ7656545.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAKMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSVTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAVLETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRT"
     gene            2891..3517
                     /locus_tag="WJL14_001490"
     CDS             2891..3517
                     /locus_tag="WJL14_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ7656546.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYIVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(3581..4999)
                     /locus_tag="WJL14_001491"
     CDS             complement(3581..4999)
                     /locus_tag="WJL14_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7656547.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            5326..6054
                     /locus_tag="WJL14_001492"
     CDS             5326..6054
                     /locus_tag="WJL14_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ7656548.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(6113..6601)
                     /locus_tag="WJL14_001493"
     CDS             complement(6113..6601)
                     /locus_tag="WJL14_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ7656549.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCAAIVLMCRPRRKAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVALTVWWAWAR
                     AK"
     gene            complement(7292..7756)
                     /locus_tag="WJL14_001494"
     CDS             complement(7292..7756)
                     /locus_tag="WJL14_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ7656550.1"
                     /translation="MKELDKIDFRILKILQQNARIPMMELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         2009 a         2104 c         1964 g         1719 t
ORIGIN      
        1 tttcccgaca ggcggatttg aagtttctcc acatatccgg cgcgttcggc gcggctggtt
       61 tcggggcggt gcgtattgag cgacgaccat ttccaatgac tgcgggcttt gttgagttcg
      121 ggcgggagtc tggcggcgtc ccacggggct ttgcggctgt gcagctcgat atccgactgt
      181 gccgcgtgtc cgcgcgtttg caggacgtgg agcaaatcga gggcgcgggc ggcgagcagg
      241 gtcagggttt cggggtcggt gtgcagggtt tgacggccgg cgagtttgtc agaaatggtg
      301 cgggtattgg gcaggatgcc gcccaaaaag ccgccgattg ccgtacccaa tccgagcgag
      361 ccgccgagtg tggcgatgtc cagccccaag ccgatgagcg cgccggttgc cgcgcccgtg
      421 ccggtgcgga tgccgtattg tttgagcaat tcgctgtcga acgggtcttg gcggaaggct
      481 tgcggcatcc agtcgccgcc gtcgatttcg ctgtggtaga aacggtaaag ggcaaacagc
      541 cgctgctgca tctgccgttc gagttggcgt atttccgcct gcatggtttg cagcacggtg
      601 gcggtatcct cgttttcgtc cacttcctgc ctgaaggcgg cggcatcaat taaaaagtcg
      661 gcgatttcgc ggcgcgcttc gccgtccagc cgctgccatt cgcgccggcg catggctgtc
      721 aggcggtcaa gtgtgctgcg ttcgggcaac atggtggcga ggttttccca caggcgtagt
      781 tcgccttcaa aatcaaaggc gacggtgtcg aaccctgcga aaacgtgcag gtttctcctc
      841 gccagcatgg ttgtccacga ttcgggaggc tgtccgccgg taaagttgaa cacgggcata
      901 accggtttgg cacaccatga aaggatggtc aactcgtccc tgtatttgtc gaggacgggt
      961 tcgcgcgcgt cgatgacgta cattgccata tcgctttgca agacttgccg taagactttg
     1021 gcttcctgat tgaaatcatg gtgcgcgccg tggctgccga gaaactgttg cagccgttcg
     1081 atgccgtctg aacgcgtatc cgtatgggtt tccagccatt ccaaaacacc gcccgcgtct
     1141 tcgagtccgg gcgtgtcata caggaaaacc agcgtgtctg cgccgtcgct gatggcggct
     1201 tcttcgacat ggcgcgtagt ggacggggca tttttgactt cgccgaagcc gctgtcgcgc
     1261 aatagggtgc gcaggagcga ggttttgccg gtattggtat gcccgacgac ggcgagggaa
     1321 aggggttgtt tgttcatgat gtttttgaag aatggatttt cagacgacct tttaaggatg
     1381 gcggctttat ggggcgtttc aagtgcggtc gttggttttc agacggcctt cctgcgccgc
     1441 tctgtcgggt tccagccacg ccgcgccgca ttcggtcagc gcgttacgcc aatgttccag
     1501 cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca ccgcgccgcc
     1561 ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg gcacagtttg
     1621 ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct ccagcacggc
     1681 aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca gcctgccctc
     1741 gaaccattcg ccgtcctgcc attcggtctc cagcatgacc gcccatttcg gcgcatcgtt
     1801 caagacgatt ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg catcggtgat
     1861 tttgttctgc cagcggcgga tgaccgcctg ataatagggc ttttccaaat ccaagccgtt
     1921 ttcgcttgtt ttcaaaagga ttttgcatac cgcccaagcc aagaggcgcg gcaggatgcc
     1981 gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg caatattgcc
     2041 gttcagacga ccttcgatga ccgcccgcgc atcaggcacg ggaaaaccca gtttcgcagg
     2101 cagccatgcc aacatttcca ccagccgtac cgaagacgaa tcgcccaaca gcgtgctttc
     2161 ccagttgaac gtatattgcc gcaccaaaag cagcaacaat accgacacca gcattccgag
     2221 cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta ccgaaggttg
     2281 ccgccactcg tccgcataca gccgcaacac cgcctgattg acagggtctt tgccccgaaa
     2341 ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca acattgccaa
     2401 ccatactgcc agcatcagcg tattcatgcc caacacgccc gccaaaacca aaaagaaatt
     2461 cagaccctga ttgtccatta gaagataagt aactgaaaaa ccggtaaaaa acgcaaacgt
     2521 cgccgccgcc acccacaacc agaacgaccc cgcacgcaca cgttccaacg tctcccgcag
     2581 catacggttc ctgtcgatca tcttcgcccg acggatgatt ttttcctccg tactgccgtc
     2641 cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa agccgccttc
     2701 ttccaaaata cggaccagct caaccagttt tcgggatgga ttcaacataa aatgccgtct
     2761 gaaaataaaa aacagatttt aacacacgca ttttcaagaa tattcacagt gtaggcaaag
     2821 agtaaatccc acacagaggc aaaagtatcg gcgtaaactg actgcctcta ctttcccgaa
     2881 agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga gcggtttgat
     2941 tctggcggcg gcaggtttgg cgctgatgct gctgaaaacc ataggacacg gggacggcta
     3001 ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt tgagttcctc
     3061 gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa ccgaccactg
     3121 catgatttat gtgctgattg ccggaagcta cacaccgttt gcactggttt ctttgagaaa
     3181 cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca
     3241 agaactcacc attggacgga aaagcgaaaa acgactgctg tctattgcga tttatatcgt
     3301 aatgggctgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc cgccggcagg
     3361 actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact ggtttgtaaa
     3421 cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg gcggcagcat
     3481 cacccaattt gtcagcgtgt acggttacgt aatctgaatg ccgtctgaaa agcaaaacct
     3541 cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg tcgtggaact
     3601 cgtggaattc atactgatag gacaaatccc gacccgcttt tttctgtgcc aaataatcat
     3661 cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc gggataacca
     3721 taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg agcgtaccta
     3781 aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc ctgaaaagaa
     3841 aacggatgtt ggactcgccg aaataatacc agccgataat ggtcgtcaag gcgaaaaagg
     3901 tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta aaggcaaatt
     3961 gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc ggtcagcagg ataatcaatg
     4021 ccgtagccgt acataccaaa atcgtatcga taaatacacc gacaaatgcc gccatacctt
     4081 gctgcacagg atgtttcaca tcagcggttg catgtgcatg cggggtagaa cccatacctg
     4141 cttcgttgga aaacagaccg cgcgccacgc cgaaacgtat cgcttcacgc ataccgatac
     4201 ccgcagcacc gcccaaaacg gcttcaggat tgaaggcggc ggtaaagatg tggttgaaca
     4261 tcggcacaat atagtcggaa aattcaaaca ggataacgac ggcgcacaaa atataaacaa
     4321 ccgccataaa cggcacgaca aactgggcaa tattggcaat acggttcacg ccgccaatca
     4381 caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc acatcaaatg
     4441 caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata aagcccaatg
     4501 cgataatcag cgcgatggag aaaaagcccg acaggaaacg cgccgcgccc ctgccgattt
     4561 tcggagtcag accgtgcgta atataaaacg ccggcccacc gatgtatttg ccgtggctga
     4621 cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc cccaaaacgg
     4681 cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg gtcgccacgc
     4741 cggcgacgtt gcccgtaccg atttgcgcgg atatggcaac cgccaacgcc tgaaactgcg
     4801 ataaagactt atcgtcttta tcgcctttgg caaacaagcc gccgaatacg gatttgaatc
     4861 ccgcgcctag cttggtaatc tgcggcgcac cgagatacag cgtaaaaaac aggccgatac
     4921 ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta cccaccagaa
     4981 cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac tgccttccaa
     5041 aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca gatgccttaa
     5101 attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt aattaaaaca
     5161 tttctttatt tttaagcgga aagcggagga aatcgctttc agacggcatc gacaacggca
     5221 cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg accgtaaaaa
     5281 atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc aggccatagc
     5341 aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg caaaatcttc
     5401 acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga tcccggcgca
     5461 aatccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc caaagacaat
     5521 gtgcaacgcg ccatcgacaa aggcacgggc aacttggaag gcgtggaata catcgagttg
     5581 cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct gaccgacaac
     5641 aaaacccgca ccgttgcgga cgtacgccac gcgtttacca aaaacggcgg caacttgggt
     5701 accgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt cgaacccggc
     5761 gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga cgtggttacc
     5821 aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg cgtaaaatcc
     5881 gctttggagg cggcaggtta caaatccgtt gacggcgacg ttacgatgcg cgcccaaaac
     5941 gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga cgcgctggaa
     6001 gacttggacg acgtgcaaga cgtttacact tccgccgtat tgaatctgga ctgatacgca
     6061 gcacagcaga catacaataa aatgccgtct gaacctttca gacggcattg atttatttag
     6121 cccttgccca cgcccaccaa accgtcaggg caaccgccag aaaatacgcc acactccaaa
     6181 caggcaaagc aacccccagc agataatccg gttcggcaca atttccgaac ccgcgcacga
     6241 caggctcgaa ccaatcaaac aaaggccagc ccttcaatcg aaacgtccac ggcgcaccgc
     6301 acgaaggagc cgtacccggc ggcagcgact gcagccacaa ctgatatgcc gcaacagaaa
     6361 tacccgtaac ggcgggaatg ctgataaaga cagcaccgaa caaaccgcct gcctttcttc
     6421 ttggtctgca catcaggaca attgccgcac acaatgcggt tgccaagacg cacaaccgct
     6481 ggctgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc aaagagccgg
     6541 caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat aacggggtca
     6601 ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat atcccgagat
     6661 ggcaagggac aaaacggcga tttgcccgca caatgcccac aaaaaaatac atccggagga
     6721 tttgaatttc agcaaattta gcgaatcaaa agtttatttc aatgaaatca tatgattttt
     6781 ttgaataagc ggattgatgg gtttttgaag gaatttgtta ccggatagcc atcgggcaag
     6841 ttttttgcaa aatttgaatc ggtcgggtaa attttcaaaa aataattgac agcggataag
     6901 aaacggcgga taattcccgc cgtcgagttg cttgatgcag cttgattttt ctcctctatt
     6961 tctcctttgt agacttggca cacattcaac tggatgtgtg cattttttta tctccgcttt
     7021 ttttgaaatt taattacttt tattgtttga aattccattc tttaagaaat ttaacaagaa
     7081 aaacgcttgc tttttgaccg gaaaagctgc ataattcatt ccgttagctg gttcgagtag
     7141 tcagttaata gtttctcctc tatttctcct ttgtagactt ggcacacatt caaccggatg
     7201 tgtgcatttt tttatctgaa gcaacaagcc tctgtgcgtg atgttgttat gtttcattta
     7261 ggtgtcaaac cgcatagcca gcctgaaata ttcaatccaa atccaaaact ggattttctt
     7321 tgacctcctc catcacaaca taactcctac tctccgaagc ggcaggcagt tgcaatagga
     7381 tattgcctag catatcccga taggcagaca tatcgggcaa acgtacttta atcagatagt
     7441 cgtattcgcc cgacaccaag tggcattcca taatttgcgg aattttcagc acttcttttt
     7501 tgaaatcttc gaaaatattg cccgatttgg attgcagctt cagctcgaca aaaaccaata
     7561 gtggtttgcc caacagatgg ggattgagat gggcgtgata gccggaaata taatgttccc
     7621 gctccaaacg gcgcaccctc tctgtaacgg gcgtggtgga caagcctacc ttctcggcaa
     7681 gctccatcat cgggatgcgg gcattctgtt ggaggatctt aagaatgcgg aaatcgattt
     7741 tatctagttc tttcatttgg attgccttgt atttattatt gattttaaca aataga
//
LOCUS       DAWKQB010000095         7791 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.095, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000095 DAWKQB010000000
VERSION     DAWKQB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.095"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(86..766)
                     /gene="tsaA"
                     /locus_tag="WJL14_001495"
     CDS             complement(86..766)
                     /gene="tsaA"
                     /locus_tag="WJL14_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ7656551.1"
                     /translation="MTYTIVPIATARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            881..1516
                     /locus_tag="WJL14_001496"
     CDS             881..1516
                     /locus_tag="WJL14_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ7656552.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDMVIVGGLATDYCVKTTVLQL
                     LKGSNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKYINNQ"
     gene            1553..2521
                     /gene="waaC"
                     /locus_tag="WJL14_001497"
     CDS             1553..2521
                     /gene="waaC"
                     /locus_tag="WJL14_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ7656553.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKMNLLQAAYLLKHAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            2747..3496
                     /locus_tag="WJL14_001498"
     CDS             2747..3496
                     /locus_tag="WJL14_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ7656554.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLATDILPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3507..4442
                     /locus_tag="WJL14_001499"
     CDS             3507..4442
                     /locus_tag="WJL14_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ7656555.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKVDLLVAGSGASAVV
                     ESAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTIFVRPIYAGNAFETVQVDSEKLVLTFRATAFDAAPVQGGH
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4505..5116
                     /locus_tag="WJL14_001500"
     CDS             4505..5116
                     /locus_tag="WJL14_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ7656556.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(5168..5485)
                     /locus_tag="WJL14_001501"
     CDS             complement(5168..5485)
                     /locus_tag="WJL14_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ7656557.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5642..6913
                     /gene="purD"
                     /locus_tag="WJL14_001502"
     CDS             5642..6913
                     /gene="purD"
                     /locus_tag="WJL14_001502"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ7656558.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6947..7549
                     /locus_tag="WJL14_001503"
     CDS             6947..7549
                     /locus_tag="WJL14_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ7656559.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
BASE COUNT         2066 a         1528 c         2086 g         2111 t
ORIGIN      
        1 ctataacttg atattgcggc ttatggcgaa caaacaacag ccgtttgttc aactgctatg
       61 cggcaaaacc ggatttttgc ccgatttaaa gcggggttgg ggaaagatca ataacggttg
      121 cacggttttc ctcgatttga aatctgattt cgtaatccgc aatattcatc acataaatcc
      181 gttcgggaat attctgatag gcggggcgcg gatcttgggc aatgctttgg ctgataaggt
      241 ttttggtgtt tgcagataaa ttttccgcgc cgatgttttc ctgccaaacg acttccaact
      301 ctacgggttt gccgctgacg aaaccagatg cggcatcggg tttggattcg acaaagggga
      361 tataaggctt gatgtccaca atcggcgtgc cgtcgagcag gtctgcgccg ctgcaataaa
      421 ggcggacggg tttgccggtt tcgatgcggt tgagttttaa gagtgatagt ccgagatggt
      481 tggggcggtg ggggctgcgc gtggcgaata cgcccatttt ttgtttgccg ccgagccgtg
      541 gcgggcgcac catttgtgcc cagccttcat ccaatacgcc gtgaaaaata aaacttatcc
      601 acacataatc gaaatcttcc agcccgcgca cgctgtctgc ggtaaattcg ggattcagct
      661 cgatgcatac ttctgcggcg gagactaaac cgggctggcg ggcgatgccg aatttctgtt
      721 tgtagggcga tcgggcggtg gcgataggga cgatggtgta agtcatgagg attgtggata
      781 agtttttcgg aaaaacctta tcttatcaca gcctgtttta ccgcttttgg gcagtaattt
      841 gttataatac agggcatttt aattgcgttg aaatgatgag atgattgttt ccattgatgt
      901 tgatgcacaa aaaactttta cgcctttgtg tcctaaagag ctgcctgtaa acgaggggca
      961 tttgattgtc gaagaattga atgcccaagc cgctttggcg gatttgcgcg tgatgacgaa
     1021 agatgcgcat catatggtag cgaaatggct tgtggataac cctgttgata tgttgaagcc
     1081 gacaggtttg cctgatgcgg atttgacttg ggtggctcat gcgatggtcg gtacgcgcgg
     1141 ctatgaatta ttggacggac tgccttctgc caaagaatac gattattgcg tttggaaagg
     1201 tgttgatcct gaattgcatc cttacggcgc gtgttttcac gatattgagg aaaaactgag
     1261 cactggattg attgagtggc tgcgttgtca aaatacggat atggtcattg tcggcggttt
     1321 ggctacggat tattgtgtta aaacaacggt tttgcagttg ctcaaaggta gcaattggca
     1381 ggttatcgtc aatgaagcgg cttgtcgggg tattgcgccg gataccattg aaacggcatg
     1441 gcaggaaatg cgttctgccg gtgctgtcat cttaaaaaac gccgaagaaa ttaaaaaata
     1501 tattaataat caataagtta aaatcgtttc acgtgaaaca tacttcttaa atatgaaaat
     1561 tttgcttgtc cgcttgtcta gtatgggcga tttaattcac actttgcccg caatcgaaga
     1621 tttggcgcga caatgtcctg atgtagaact gcattggcta tgtgaggctg gatttgcaga
     1681 tattgcgcgc ctgcatccgt ttgtaaaaaa aatccatgtg atgaaatggc ggcaatggcg
     1741 caaacatctc tttcaggctg aaacttggcg ggaaatgggt catctgaaac agactttgcg
     1801 gcaggaagca tttaattttg tattggacag tcaaggtctg attaaaagcg cgtgtttcgc
     1861 taaaatggcg aaatccccta tttatggttt ggataaaaat agcgcgcgcg agggactggc
     1921 tgcgctggcg tatgcgaaaa catacgctgt accgaaaggg aaaaatgctg tttggcgcaa
     1981 tcgtgagctg ttcgcccaag tatttgggta tgcgatgcct gaaacgcagg tatttggctt
     2041 gactgttcct gaggcaggcc gtctgaaaaa tttagcgcag ccgtattatg cggctttgca
     2101 tgcgaccagt cgggacagta agttatggcc tgtggaaaac tggcggtcgt tgctgcaaaa
     2161 gttgaatgaa gaacagcaat gcaatattta cctgccttgg ggaaatgaag atgaaaaaac
     2221 gcgtgccaaa caaattgcag acggactgcc gtttgctatt gtgtgcgata aaatgaattt
     2281 attgcaggca gcgtatctgc tgaaacacgc ggtcggaatt gttggcgtgg ataccggttt
     2341 gctgcatttg gcaaacgcct tggaaaaacc tgtggtcggc atttataccg ataccgatcc
     2401 gattaaaaca ggcgttcagg tttcgccaat tgcaaaaaat ttgggcaata tcgggcagat
     2461 tccgaccgca gatttggttt atcaaacgtt gatggattgt gttgcagcag acaaaggcta
     2521 aagggttgtc tgaaactgat atagcacctg atatttttca gtttgttaag cataagaatg
     2581 tatatctacc agcctgagta aatgctttaa aaatggtata atcggcaagc attatccgaa
     2641 agatttttga atgaattcgt ttttaaggaa aacggatttg gttttatagg gaagaaggtt
     2701 tggtttcgct ggatggtgaa agcagcgttc tttttaggag ggttcaatga aagcactggt
     2761 cgcagtaaag cgcgtagtgg actacaacgt caaagttcgt gtaaaagccg atggttcgga
     2821 tgtggatatc ggcaatgtca aaatgtcgat gaatccgttt gacgaaatcg ctgtggaaga
     2881 agctgtccgt ttgaaagaag ccggaaaagt aagcgaaatc gtagcggttt ctttgggtga
     2941 gaaaaaatgc gaagaaacct tgcgtacagc tttggcgatg ggtgccgacc gtgccattca
     3001 tgttgaaacc gatactaaac tggagtctct ggccgttgct aagttgctga aagccgttgc
     3061 ggacaaagaa aatccgcaaa ttttcttttt gggcaaacaa gcgattgatg atgatgccaa
     3121 tcaagtggcg caaatgctgg cagctttgct gaatgcggcg caaggtacgt ttgcttcaaa
     3181 agtacacatt gaaggtggtg aagtacagat tgtgcgcgaa attgatggcg gcgaagaaac
     3241 catagcatta aaactgcctg ctgttatcag tgctgatttg cgtttgaacg agccgcgctt
     3301 tgtcaaactc cccaatatta tggcggcaaa gaaaaaacct ctggaaaaac tggcgcctgc
     3361 cgatttggct accgatattt tacctcgtct gaaaacggtg aaatttgccg aacctaaggc
     3421 gcgtcaggca ggcgtaaaag tagcaagcgt tgccgaattg gttgaaaaat tgaaaaacga
     3481 agccaaagtg atttgaggag gctgaaatga gcgtattgat tattgtcgaa cacgacaaca
     3541 aacagttgaa tcctaccact ttgcatgctg ttaccgctgc cgccaagctg ggtaaagtcg
     3601 atttattggt tgccggaagc ggtgcatctg ccgtagtaga gtccgcaaaa caagtggcgg
     3661 gtgtggaaaa agttttggtt gcagatgctg cccattatgc cgaaggtttg gctgaagagt
     3721 tggctccgct ggttgttaaa ttggcagcgg attaccgcta tgttgcagca acggcaacta
     3781 cattcggtaa aaaccttttg cctcgcgtag cagccttatt agatgtaccg caaatttctg
     3841 atttgaccga gattgtggat aacacgattt ttgtgcgccc catttatgca gggaacgcat
     3901 ttgaaaccgt gcaagttgat tcggaaaaat tggtgctgac cttccgtgcg acggcttttg
     3961 atgcagctcc tgtacaaggt ggtcatgccg aggtaattaa tgttgaagca acccctgccc
     4021 aaaacctgag ccgttttgtt aaccgtcagc tttcccattc cgatcgtcct gaactgactc
     4081 aggcaaaagt gattgtttcc ggtggtcgtg cgttgggtag tgcggaaaaa ttcaatgaag
     4141 tgctgacacc gttggcggat gttttaggtg cggcaatcgg tgcatcccgt gcggcggttg
     4201 atgccgaata tgcgccaaac gatgctcaag tcggacaaac cggtaaagtg gttgcgccgc
     4261 aactctattt tgcaatcggt atttcaggtg cgattcaaca cgttgctggt atgcaagaca
     4321 gtaaggtgat tgttgcaatc aataaagatg ctgatgcgcc gattttcaat gtagccgatt
     4381 acggattggt tggcgattta tttgaaatcg ttccacaatt aacagaagca ttgaaaaatt
     4441 aatgtttcac gtgaaacgtt cagacgacct tgttattaat gaggtcgtct gaaaacattt
     4501 cagcatgatt acgacttata aaaccattac ttcctcgacg caggctgagt ttaaagataa
     4561 aggcagccgt tttattgcat ttgcctatcc gattcggaca ttggctgatg tgaaaaaata
     4621 cctcgatccg ttaaaggaag agcatcataa agcacgacac tggtgctatg cctatcgttt
     4681 gggtgtggat ggcatgcaat ttcgtgccaa cgatgatgga gagccatcag gaagtgctgg
     4741 acgaccaatt ttgggacaga ttgattcggt gggtattacc gacgttttgg ttgtggtcgt
     4801 ccgctatttc ggtggtactt tgttaggtgt tccaggtctg atacatgcgt acaaagaggc
     4861 gacggctcaa gcgttggcag ttgcagaagt agttgaaaag aatattgaaa aaactgtttg
     4921 gctgaaatgt gaatatccgt ttttgaatga agcgatacgc attgccaaac aatatcaggc
     4981 ggatatattg gagcgggatt tgcagttgga ttgccgattg acggtcagtt tgtcgttggt
     5041 aaattatgag gcctgtgttg cggcttggaa gaatactcgg cagattgaag taaatacgga
     5101 aaagcctttt gaatgaaagg tcgtctgaaa tgctttgtga tggtatttca gacgacctct
     5161 tcgctgacta atcttcttgt gtatatttgt ctaaggcatc gacgctggag cgcaaatata
     5221 accaagccaa ttgatggaat tcgcccagcg cgttccatag cgcatcttca ctcataatgc
     5281 tgtattcgcc ttccgtactc aaatccagtt ctaccccgtc ttcaatagcc tgatgacacg
     5341 cctgatactc gtgggtatag aaattgtcca tgtcgcgaat taaagtgacg acatatttcc
     5401 aacggcggat gtcttcttcc agttgcggta gtaaatgaca ccattcgcgg tatttgcttt
     5461 ggatttcatc aatacgtttt tgcatggttg cttgtccttt tttaatttgt gaataagttg
     5521 aataggttgc atagtggaat gaagtggtct ttgtttgatt ccaccaaaat ttcttcagat
     5581 gggacaaaag tccctttgta tcgagttttc gttaaaagta tatatttgtc aaggagatgg
     5641 catgaaactg ctggttatcg gtaatggcgg tcgcgaacac gcgctggctt ggaaattggc
     5701 gcagtcgcct aaagtggaaa cagtatttgt tgcgcctggt aatgccggta cagcgattga
     5761 acccaagctg caaaacatcg acttgactgc gcatcaggat ttgattgaat tctgtcgtaa
     5821 agaaaatatt gtttttaccg tcgtcggccc tgaagcgcct ttggcggcag gtgttgtgga
     5881 tgatttccgt gctgcaggac taaaaatatt cggtccgaca caatatgcgg cgcagttgga
     5941 aagttccaaa gatttcgcca aagcatttat ggcgaaatac aatattccga ccgcgcaata
     6001 tcaaaccttt gaaaacgccg atgccgcaca tgattacgtc aatcagaaag gtgcgcctat
     6061 cgttatcaaa gccgatggtt tggcggcagg taaaggcgtg attgtggcga tgactttgga
     6121 tgaagcgcat gctgcgattg atgatatgct gctggacaac aaaatgggca atgccggcgc
     6181 gcgtgttgtg attgaagatt tcctgcaagg cgaagaagcg agttttatcg tcatggttga
     6241 tggcaataat gtactgccta tggcaaccag ccaagaccac aagcgtcttt tagacggcga
     6301 caaaggtctg aatacgggcg gtatgggtgc gtacagccct gcgcctgtgg taacgcctgt
     6361 tgtgtacgaa cgcgcgatga atgaaattat tttgccgacc gtagcaggga tgaaagcgga
     6421 ggggcatgag ttcaccggtt tcctgtacgc aggtttgatg attgatcaaa gcggtgcacc
     6481 ccatacgatt gagtttaact gccgtttcgg cgatcctgaa acccagccga ttatgagccg
     6541 actgaacagc gacttatcgg atttggttga agcggcaata gacggcaaac tcgatagcgt
     6601 aaccgcggaa tggagcccgc aaactgccgt gggcgtggta ctggcggcgc aaaattaccc
     6661 cgaaacgccc aaaaaaggcg atgttatttc cggtctggat gctgccaacc aagtaggcaa
     6721 agttttccat gcaggtacaa cggcaaacga gaaaggtgac gtattgacca acggcggacg
     6781 cgtattgtgt gttgtgggat tgggcgataa tgtggcgcaa gcgaaagcta aggcatatgg
     6841 cgcgttggaa aaaatcagtt tcgacgggat gcaataccgt aaagacattg ccgataaagc
     6901 gattaaccgt taattagaaa gaggatgatg ccgtctgaag ccaagtatga ctgatgtgct
     6961 tcagacggta ttttatatta tgttatttcc gagaattatc gcagcatcag cgcaacgtaa
     7021 gttaagcgtt tatttgaaaa aaggcggttt ggtcgcttat ccgaccgaat cttgttatgg
     7081 cttggggtgt cttccgacat tagccaaagc actcggtaag ttggcgcatt tgaaaaaaag
     7141 accgcaacac aagggtatga ttgtcatcgg gaatcaattt gagcaattgc agcctttact
     7201 tcaaatgccg tctgaaaatc tgcaggatat gcttagaaaa gaatggcccg cccctaaaac
     7261 gttcctatta tccgccaaat cctgtgtctt acctgagttg cgcgggaaac agcgcagcaa
     7321 gttggcggtc agggttcccg cccatgtagg tgcgcgccgc ttgtgtcagg ctttgcaaac
     7381 gcctttggtc tcgacttcgt gcaaccgcgc aggcaaacgc gcgtgtcgga cggagaggga
     7441 agtcagaagg caattcggac gcgatgtgtg gattgttggt gggagaatcg gcaggcagaa
     7501 atcgccgagt cagattattg acggggaaac aggtaaacgt ttgcgttaga tatgggtact
     7561 tgaatgaaaa ctttttttgc atacacaaag aaaaaatttt tcggatagca tttctctaca
     7621 tgattgttat tgcacagaga taaaggtaga aggacgcaac gtctgcttta attttgagga
     7681 cggttttgtc gttccggaca ataatccgaa caatcaagcg ggcaagcatc tgaaaacaga
     7741 tttttcccgt gttgttttaa tccataaaaa tgaaaacgcc gaagatgatt a
//
LOCUS       DAWKQB010000096         7784 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.096, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000096 DAWKQB010000000
VERSION     DAWKQB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.096"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            106..434
                     /locus_tag="WJL14_001504"
                     /pseudo
     CDS             106..434
                     /locus_tag="WJL14_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            446..712
                     /locus_tag="WJL14_001505"
     CDS             446..712
                     /locus_tag="WJL14_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656560.1"
                     /translation="MDFEFGFKTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADSTNIAAALERIENKLEKVNEKLDRKADKS"
     gene            709..906
                     /locus_tag="WJL14_001506"
     CDS             709..906
                     /locus_tag="WJL14_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656561.1"
                     /translation="MSDPILEVLARIEAKQDDMLANQARMDEELQQIKKDCKKSAAVY
                     GGLGGVIVTTGWELLRAKFGG"
     gene            908..1414
                     /locus_tag="WJL14_001507"
     CDS             908..1414
                     /locus_tag="WJL14_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7656562.1"
                     /translation="MAHPKEIREKLRRLYVSDGQTLEIAAMMCEIPIATARSWKRAAK
                     ESSDDWDKVRAAYTLAGGGIEDLSRSLLAGFLVQYQSTMTMLQDTSIEGLMPSERAKL
                     LTSLSDAFTKTVAANAKVMPETSKLATAIEVLELFGEVVRERYPQHLQAYVELVEPLG
                     VEIEKKYR"
     gene            1435..3057
                     /gene="terL"
                     /locus_tag="WJL14_001508"
     CDS             1435..3057
                     /gene="terL"
                     /locus_tag="WJL14_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ7656563.1"
                     /translation="MKSKEFLKSLAEYAAQLRQIIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            3057..4625
                     /locus_tag="WJL14_001509"
     CDS             3057..4625
                     /locus_tag="WJL14_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ7656564.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            4612..5907
                     /locus_tag="WJL14_001510"
     CDS             4612..5907
                     /locus_tag="WJL14_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEZ7656565.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDMERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLNIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            6017..6433
                     /locus_tag="WJL14_001511"
     CDS             6017..6433
                     /locus_tag="WJL14_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ7656566.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            6664..7725
                     /locus_tag="WJL14_001512"
     CDS             6664..7725
                     /locus_tag="WJL14_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656567.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
BASE COUNT         2035 a         1890 c         2199 g         1660 t
ORIGIN      
        1 cgccgccgag tttggcggct atgttgccga actggaaaat caaaatcagg cttggcgcga
       61 ctgggtcaac agtcaagcgg cagttgacgg ttcggagggt gcgcgatgac gacttatcgt
      121 gagttggtgc aacgcacggt cgcctgccgc catgcggatt tagagctggg attgagccgc
      181 gcacgcgagc aagagccgtt tgtcattcat gtttccgatt tgttggataa ggccggtatc
      241 gattacgcgg tacgcatgga taaggatttt cagacgacct tttgtgtgga gttttccgcg
      301 acgcccctgc tgatgtgatt ggtattttgc ggaaatatta ctcagtcttt tcagacggcc
      361 aaaaggtcga ggcggcgagt cgtcatcccg aaggctacgc ggtccgtatc gtatttggcg
      421 atgtgccggt ttaaaggggt tttaaatgga ttttgaattt gggtttaaaa ccctgtggcc
      481 gattgcaacg gcggcgtttt ggttttgggt caacggcatt tcaggccgtc tgaaagaggc
      541 ggacaagcgc attgacgacc ttaaagagga gctgcacgcg gtcaagctct cttatcacac
      601 caagcaagat gccaaggcag acagcactaa tattgcggca gccttagaac gaattgaaaa
      661 caagttggaa aaagtaaacg aaaaattgga caggaaagcg gacaaatcat gagcgacccg
      721 attttggaag tcttggcgcg tatcgaagcc aagcaggatg acatgctcgc caatcaggct
      781 cgaatggacg aggaattgca gcaaattaag aaagactgca agaaatctgc ggcggtttac
      841 ggcggtctcg gcggtgtgat tgtaacgacc ggctgggagc tgctgagagc caagttcggg
      901 ggctgatatg gcgcatccga aggaaatccg cgaaaagctg cgccgattgt acgtcagcga
      961 cgggcagacg cttgaaatcg cggcgatgat gtgcgaaatc ccgattgcga ccgcccgcag
     1021 ctggaaacgc gccgccaagg aaagcagcga cgattgggac aaagtgcgcg ccgcctatac
     1081 cttggcaggc ggcggcatcg aagacttgag ccgttcgctg ctggcgggtt ttttggtgca
     1141 gtaccagtca acgatgacaa tgttgcaaga cacgtcgatt gaagggctga tgccgtccga
     1201 gcgcgccaaa ctgttgacaa gcctgtccga cgcgtttacc aagaccgtgg cggcaaacgc
     1261 caaagtgatg ccggaaacgt caaaactggc gacggcaatc gaggtgttgg aattgttcgg
     1321 cgaagtggtc agggagcgat acccgcaaca cttgcaggct tatgtcgagc tggtcgagcc
     1381 gttgggtgtg gaaattgaaa agaaatacag gtagcaactg cataaggtaa ttgaatgaag
     1441 tccaaagagt ttttaaagtc acttgccgaa tatgccgccc aactccgcca aattattgag
     1501 gcagaggtgg acggcttcga tgcgtcgccc aaggctattg ctgcacgccg tgccaaggtg
     1561 tttgacccgg taggcggtta cgagtatttc gtcaacacct actttccgca ttatatccgc
     1621 tcccctgaaa aatccgaact gcatgcgttt ttattcagcc gtctgccgga gattatccgc
     1681 tcccccaaag gggaaaatga ggcggtgggt gcgccgcgtg gcgagggtaa gtcgacgcag
     1741 gttactcagt tgtttacgct gtggtgtatt gtgacaggcc aaaaacatta tgccgttatt
     1801 gtgatggaca gcatagatca ggcatacccg atgctcgaag ccatcaaggc ggaattggaa
     1861 tttaatccgc gcttgaaaac cgactttccg gaagtatgcg ggcagggccg cgtatggcag
     1921 gccggtacga ttgtgacggc caatgacgtt aaagtccaag tggccggtag cggtaaaaag
     1981 ctgcgcggtt tgcgtcacgg cccttaccgt cctgacttaa ctgttttgga cgatattgag
     2041 aatgacgagc aagtccgcaa ccccgaacag cgcgacaagc tcaatgcgtg gctgactaag
     2101 actgtattgc ctttgggcgg tgtcggccag aaatacgatg tgatatatat cggcacgatt
     2161 ttgcattacg acagcgtact taaccgcact ttgaataacc cgttttggca cggtattaag
     2221 tttaaggcga tgaaacgctg gcccgaccgc atggatttgt gggacagatg ggaggaactt
     2281 ttccgaaacg acggcgagac tgtggccgag gcgttttatc tcgccaataa agacgagatg
     2341 gagcgcggtg cgcaaacaag ctgggcggct cgcggcgtac tcgcgctgat gaaaatccgt
     2401 gcgcgtgacg gtcatgcgac atttgacagc gaatatcaaa acgacccggt cagcggcgaa
     2461 gatgcgccgt ttgccaagtc gatgaagttt tggaacgacc tgccgtccga tttggtgtat
     2521 ttcggtgcgc tcgacccgtc gctgggtaaa gcgggggcga gccgtgaccc gtcggcgatt
     2581 atcattggcg gttatcaacg tgaaaccggc aaactgtatg tcgtggaagc tcagattaaa
     2641 aaacgtctcc ctgatttgat tattgaggac gttatcagat tgcaccgtca atatcgttgc
     2701 aaactgtggt ttgttgagac ggttcagttt caggaatttt tgaaagatga gctggtcaag
     2761 cgcagcgcgg cgcgtggaat acctgtcccg gcgcgggcgg tcaagccggt atcggacaag
     2821 ctcttgcgga ttgagacttt acagcctcac atggcgaacg gtttgattct gttgaatgag
     2881 agccaacaga cgctgataca gcagttccgc cattttccaa aggctgatca tgatgatggt
     2941 cctgatgcag tgcatatgct ctggtcgggg gcggtggcca attgtgtgcc gatagaatgg
     3001 caaagcccta ccgataacga ttttgatgac gagataaaaa gtaaatggag ccgataatgg
     3061 caaaaaagaa caataaaact aaaatccaaa agcccgaagc tgcattgcag acggacgtgg
     3121 ctcaaattac ggcgaccggt cgggttatcg ccgagcatcc gtccaatttt attacaccgc
     3181 aaaagatgcg ggccctcttc gaggatgcgg aaagcggcga catccgcgcc caacacgagc
     3241 ttttcgcgga cattgaggag cgcgacagcg acatcgcggc aaatatgggg acgcgcaaac
     3301 gcgcgctgct gacgctcaac tggcgcgtcg ccccgccgcg aaatgcgacg cccgaagaag
     3361 aaaagctgtc cgaccaagcc tacgaaatga tggacagcct gcctaccctc gaagacctga
     3421 ttatggattt gatggacgcg gtagggcacg gattttctgc gttggaggtc gagtgggtat
     3481 tttcagacgg cctttaccta ccccgaaact ttatccaccg cccgcaaagc tggttcaaat
     3541 gggacaaaga caacgggctg ctgctgcgta cccgcgaaaa tccggaaggc gaagcgttgt
     3601 ggccgctggg ctgggtcgtt catacccaaa aatcgcgcag cgtccagcag gcgcgcaacg
     3661 ggcttttccg cacgctttcc tggctgtata tgttcaaaca ctacgccgtc cacgattttg
     3721 ccgagttttt ggagctgtac ggcatgccca tccgtatcgg caaatacggc gcgggcgcaa
     3781 ccaaagagga aaaaaacacc ctgcttcgag cggtggcgga aatcggtcac aacgcggcag
     3841 gcatcatgcc agaaggtatg gaaatcgagc tgcacaacgc tgcaagcggt acgatggcaa
     3901 ccagcaatcc gtttttgcag atggccgact ggtgcgaaaa atcggcggcg cggctgattt
     3961 tggggcaaac gctaaccagc ggtgcggacg gaaaatccag caccaacgcg ctgggcaata
     4021 tccacaacga ggtacgccgc gatttgctgg tgtcggacgc aaaacaggtg gcgcaaacca
     4081 tcacaagcca aatcatcgga ccgttcctgc aaatcaacta tcccaatgcc gacccaaacc
     4141 gcgtgccgaa atttgaattt gacacgcgcg agccgaaaga catcgcggtc tttgccgacg
     4201 ctatcccgaa actggtggat gtcggcgtac aaatccccga aagctgggtg cgcgacaaac
     4261 tggtcattcc agatgtgcag gagggtgagg ctgtgttggt gcggcaggta ccggacaatc
     4321 cgataaacag aactgcattg gcggctttat ccgcccacac cgtaccatct aaggctacgg
     4381 gcaggcatca ggaaatattg gacggcgcgt tggatgacgc gctggttgag cccgatttca
     4441 attctcagct caacccgatg gtgcgtcagg cggttgccgc acttaatgct tgcaacagct
     4501 acgaggaggc agatgccgca ctgaatgcgc tttatccgaa tttggacaac gcgaaactgc
     4561 gtacctatat gcagcaggcc ttgtttatca gcgatatttt gggacaagac catgcccgcg
     4621 cctgatttgg gatttgcctt aagtctgccg ccaaaaaagg caatcgagtg gctggaaagt
     4681 aaaaaggtta cggcggagag ctaccgcaat ctgacagcct ccgaaattgc caaagtctat
     4741 acgattgccc gcatgaccga cttggatatg ctcaacgaca tcaaaacttc gatggttgaa
     4801 tcggcaaaaa gtggacagtc gtttgacgat tggcgaaaag gtatcttgaa tctgctcagc
     4861 aacaagggct ggctgcatcc gaacgggcat aacggtaagg atatcatcga cccagccacc
     4921 ggcgaggtat tcggttcgcc gcggaggttg gagacgattt accgtaccaa catgcaaact
     4981 gcctacaacg ccggtcaata tcaaggatat atggcaaata ttgatgcacg accttattgg
     5041 atgtatgacg cggtaggcga cagccgcacc cgtccggcgc attcggcaat agacgggctg
     5101 gtgtaccgct acgacgaccc gttttgggca acgttttacc cgcccaacgg ctacaactgc
     5161 cgctgctcgg tcatcgctct gtcggagcgg gatatggaac gccaggggcg gattgtcggg
     5221 caaagcacgg cggacaatct ggtcgagacc cataaaatct acaacaaaaa aggcgatact
     5281 tatctgaccc ttgcctataa agcaccggat ggcagtctgt acacgaccga tcgaggattt
     5341 gattacaacg ccggacgaat gaactaccgc cccgatttag acaagtacga ccgtgcgttg
     5401 gcgcatcaat ttgccaaagc ggaaatgggt ggtgcggatt ttaaaaccag ctttaaacag
     5461 cttgaaaaag agttttatga agtcaagcaa cgtttgaata ttgatggcaa gcccgataaa
     5521 gagcagaaaa tcaaaatccg aaacgcgcta tcaagacagc ttaaatttgc tgcgggtgta
     5581 ttgagcaagg aaatgcaaga actggcaggt atgactcgag cgacggtgtg gctgtctgat
     5641 gatacgttgg ttaaacaggt agacagccgt gaggggcaga atttcgatga ctcctactat
     5701 gcttttttgc cggatatgct gcaaaaccct gaacatgtca tccgcgacaa tcgtgaattg
     5761 attttcacag ctcgctataa aggctcggca ttgtgggcag ttttaaaata tattaaggag
     5821 gtggatgaga tttatctaca gtcgtaccga atcagcaacg acaaagagat tgccaaattt
     5881 atggcgaaga agaaagtatt gaaatagacg ttgggcaagg ctcgaattca cttgcacacg
     5941 ctctcgtaca ccctaaaggg caggctgcgg tatcgagatt atcaccgctt ttccaacgcc
     6001 tataaggaat cataccatga ttgatgtcaa aatagacaat atctttgtcg tcctaaacca
     6061 aatcgagcgg cttggcaacg ggatcgaaaa ccgctacctg ctgatgcgcc gactgtccga
     6121 aaccatgcac acggcggtca agctcaattt ccgctacgca ggccgtccga aatggttggg
     6181 gctaaaatac cgcgacggca agccgctttc ggattcgggt cgtctgaaag acagtttttc
     6241 cacactgtca gacaacgata cagcccttgt cggtacgaat atcgtctatg ccgccatcca
     6301 caacttcggc ggtatggcgg ggcgcaaccg caaagttcgg attccgcaac gggaattttt
     6361 gacgctgacg gacgacgaca aacaggcttt gatggacgat gtgcaggatt atttttcggg
     6421 tctgataccg tgaatttata aaaccctcaa aaacgcgctt tttagcgcgt ttttttatgc
     6481 gggtaataca aacccctgcc caagatataa aaatcaatcc tagacgcttc taaaaagccc
     6541 ctgaaaacga ttaattgtgt atcgcgcgga caggttttaa aaaaatggcg ggagggtttg
     6601 aagcacgcct actctttgtt gttttttcaa ataggcaaaa tgaccgtatt gagagaggta
     6661 cacatgtcca aaaatgcaca aaaaacccta cttgccgtgt gcagtttcga ggtgcagcca
     6721 aaagacgggc gaatccaact gctgccatat ggcgaatttc gcgcagtaga cggtcgtccg
     6781 actgatgtcc ctgcgtggta tctgaccgaa gaaaacggtc atgatgtcgc gttgttggcc
     6841 aacagctcgc gcaatcagtt ggttgtcgat tatgaacacc agacgctcta caaagagaaa
     6901 aacggacaac ctgcacctgc cgccggttgg atgcgttggc tggagttcac gcctaaaggc
     6961 atgtttgccg aagtggagtg gacggacaag gcggctgcgg caattgccgc aaaagagtat
     7021 cgctacatct ctgctgtgtt ttcctatgac acaaagggat atgtaagcaa aatttttcac
     7081 gccgcgctga caaatttccc cgcgttggac ggtatggacg aggtgctggc ggcagcgtcg
     7141 gcgcaaattt taaaaccgga aacggagcaa aaccctatga aagagttgtt acagcaactg
     7201 ttcgacctgc ctgatgcggg cgaagaagaa ctgaaggcgg cattgtccgc gctcgtggaa
     7261 gccaagccga aagacgtggc attgtctgcc gacgtgttcg cgcagctggc ggaaaaagac
     7321 agccgcatcg cggcattgac ggcgcaaacc gccaagcctg atttgactaa atacgcgcct
     7381 atctcagtgg ttcaagagct gcaaagcaaa gtcgccgcgc tgactgccaa gcaggaagca
     7441 gacaaaggca acgaattgat taccgccgcg ctgacttcag gcaaattgct gcctgctcag
     7501 aaggagtggg caaaaggcgt attgaaacag ccgggcggct tggcattttt gaccggcttt
     7561 attgaaaacg cccagccggt cgctgcactg gcaggctcgc aaacgggcgg caaagcaccc
     7621 gacgaacgcg tcgccgcact gactgcggaa gaggcagccg cagcaaaaat gctgggcatg
     7681 tccggcgaag aatttgtaaa aatcaaagaa agcgaaggta agtaatggac aaatcagcga
     7741 ttttgaccgc aatcacggca gcattccgca aagaatttca aacg
//
LOCUS       DAWKQB010000097         7728 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.097, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000097 DAWKQB010000000
VERSION     DAWKQB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.097"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(99..494)
                     /locus_tag="WJL14_001513"
     CDS             complement(99..494)
                     /locus_tag="WJL14_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656568.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(478..795)
                     /locus_tag="WJL14_001514"
     CDS             complement(478..795)
                     /locus_tag="WJL14_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ7656569.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKS
                     ENVIDCFSFFWNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDD
                     VFV"
     gene            complement(923..1201)
                     /locus_tag="WJL14_001515"
     CDS             complement(923..1201)
                     /locus_tag="WJL14_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656570.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(1208..1492)
                     /locus_tag="WJL14_001516"
     CDS             complement(1208..1492)
                     /locus_tag="WJL14_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656571.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(1500..1697)
                     /locus_tag="WJL14_001517"
     CDS             complement(1500..1697)
                     /locus_tag="WJL14_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656572.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(1702..1992)
                     /locus_tag="WJL14_001518"
     CDS             complement(1702..1992)
                     /locus_tag="WJL14_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656573.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGRSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQPVKKG"
     gene            complement(2037..3381)
                     /locus_tag="WJL14_001519"
                     /pseudo
     CDS             complement(2037..3381)
                     /locus_tag="WJL14_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            complement(3467..3682)
                     /locus_tag="WJL14_001520"
     CDS             complement(3467..3682)
                     /locus_tag="WJL14_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656574.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(3683..4033)
                     /locus_tag="WJL14_001521"
     CDS             complement(3683..4033)
                     /locus_tag="WJL14_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656575.1"
                     /translation="MQNGGGKIYQTADNVEGIMLLKVVPERTVSADAKTRDPMWDNAA
                     LQTSEGVNFIARFLGFFSDGEYRYVDVLQPNHSDIIRYSGKDFPLNQILNHMHPARYA
                     VTFENNVDSKLRRH"
     gene            complement(3970..4312)
                     /locus_tag="WJL14_001522"
                     /pseudo
     CDS             complement(3970..4312)
                     /locus_tag="WJL14_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4548..5975
                     /locus_tag="WJL14_001523"
     CDS             4548..5975
                     /locus_tag="WJL14_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ7656576.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     VVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            6044..7447
                     /locus_tag="WJL14_001524"
     CDS             6044..7447
                     /locus_tag="WJL14_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ7656577.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
BASE COUNT         1949 a         1847 c         1888 g         2044 t
ORIGIN      
        1 cataacacct cctaatttta cccaaatacc cccttaatcc tccccggata cccgataatc
       61 aggcatccgg gttacctttt aggcggcaac aggcgcactt agcctgagac ctttgcaaat
      121 ttgtcggttt cggggtcgta ttggtagcct cgtgcctgta tgtcttcttt gaaagtttcg
      181 tatacgtcgt gggctaaaag ggctgttccg acatagggaa ccgcccttgt gctgaatttc
      241 gcgcctaagc gggcaagttt gccgaccccc gccaatacgc cggcgcggga tacgctggcg
      301 gttattttgg cgttgattcg ggcttttgcg cccgtaggga tgtgtgttaa atctaccgtt
      361 tttattaaat cagatgaata agttttacta tttttaggta caaacttatg aattttcgca
      421 ccttgtccgg tatcaactga aacagtttca gatattttta ctgcatttgc attcgcttca
      481 aacgaataca tcatcaaaat tgcaattatc gacaatttcg caaaattcaa atttgtatat
      541 tttatgacca tctttcagag attctttaat taccatttct gaattatcag aaaatgagat
      601 tagccaaata tcatgtttaa ttcttctatt ccagaaaaaa gagaaacaat caataacatt
      661 ttcagactta ttaatcttcg caaattcaac aaattcagat tgcgctataa ccgccatcga
      721 ttgcccaaaa tacttgctgg acggctgata tttataaagt gccaactgcg cctgagtgat
      781 aaacggcttg ttcatggttc tgcctttcaa tgattgtttt gaaagcctga ttttgacacc
      841 ataacttcat gcgctcaatc cttaaacaga accgccccga ttaatacggg tacggaaacg
      901 ccgagataaa aataaaaatc catcatttca aaaccttttt cagcagggaa acaaagtaaa
      961 cggacgcgag gatgccgaat actatccagc ctgtttcaag accgctttgc aggttgtctt
     1021 tcggactgca ttccgccaat aaaagcctta gcggctgacc gtccgacatc ttccacaggc
     1081 tgccgttata ttccggcctg acaatctgtc cgttttcttt gattcttggt actaccaagc
     1141 tgaaataaag gttttcagcc tggtgcttct caagacattt atttccgact tggtagtaca
     1201 tgccgtctta cttcatcact ctcttaacga tggaaaagac aaaaagcgcg gcgaaaacgc
     1261 ccactacaat ccaaccggct tccataccgt ccgcttttgc ggcttccaaa gcgttttttg
     1321 ctgtatcggg caacgttgca tttgcatgtg cggccaaagc caggggagca gctgttacaa
     1381 cagccagttt tgcgccgtat ttacggcagg tgttaataaa tttcatgata ttttccttca
     1441 aaaagtgttt ggcggtaatg gatggagcgt ttttcagacg accgccgaac atccgaaaat
     1501 cagtctttca aaaatccgaa tacgacaaat tcgtattggt tgccgatttc ttccaaacct
     1561 gcgttaatcg cttcttcgaa gtcgtagaaa taatcggcat tggtgattaa tttggtatgt
     1621 ccgatgtcgc ccgtttcagg agagagatac agaaagtccc ctgttgatac ggactggaca
     1681 acatagactt tctgcattca atcagccttt cttaacgggt tgaaaaccga tgactttcag
     1741 tttttgggtt ttgcccgtag tgacgatttc tacgttcagg tttgcttcga tgggaaattg
     1801 ggcgtttcgg aactgctcga aattggcaga gccgccgaaa tcgtattcag tagtagagct
     1861 gcccaatgcg ttgccttggg agctgtctaa gggtgtggcg acaatcaggc ggcaatagtc
     1921 gaagctcctg ccttcgattt gtccgttgaa ttttttaacg ccgacgatgt ggccttgaag
     1981 ttggatgttc attttttggt ttccttgtgt gattaaacgt ctttcgggca gacactttaa
     2041 gcccatgaaa tcggtagtct tgcgaatttg tcgtaaatga agttgttata gctttcttca
     2101 ttgttgacgt gtttttgctg ttcaagctgt ttttcaagat tctcgtaata ttcgtacata
     2161 tagtaagggt ctttgtacgg tttgaatgcg ggctgttcat gaatggcttg agctttcaaa
     2221 aaggcgcagt cgtaggcttc gggagccaaa gacttgggca gcttgtgatg actcggctca
     2281 atcagttcaa acagtttggc tttgtccaat tcgggaaaaa tgaatttcag accgtttgcc
     2341 gcacgtccaa actgtttttt tacccattca aggtagcggt cggctgaaat gaccttatct
     2401 tccttaaccg cgtgtatgcg cgttgccttt tggacgaatc gttcgcaaat cggatatgcg
     2461 ccgccgaaat attcgcccgg attctgcaaa acttcgaaag ggataacgat atcttttgct
     2521 ttgaattcaa tttcaaatcg cgtccatgtg cttgttttat cgcccaactg cttgcctttt
     2581 tcatagacgc ggacatattt ggacgattca cgggagccga taccataggt cttgcctttg
     2641 gtcattttgg cttcatcgtc ttcttcccaa tctgacccca aacattcgcc ttttggtttg
     2701 acgtgatgac aggtaaacat acctttattt cggtcttcac gggcttggtt cgggctgtat
     2761 tcgccgttga aaaagtcttt tgcgatatca acgcgtgtta tttttgggcg gattgcatta
     2821 gtcaggaatg cgaaaagtcg tgattcccag ccttcttttg cgacgccgca accggtgccg
     2881 gtcagttcga aaagaatggt attgttttgt ccgccaaaat ggacgcgacc gtataggacg
     2941 tcttccgaac ccatcaacca acagcgctca taaaaacgcc cgcccgaacc tttggactct
     3001 ttgtagatac cgaaaccgaa aacttcttcg gcgagcatgg acgcggcgcg aataaaatct
     3061 tcgtcttcca aaagacttac acgaacgccg tatttatcga aaaagggttt ttcatgaaat
     3121 gaaaagctaa tttgatcaat gaaagccgaa tctgatacac cgcgccgaag aggaacgcct
     3181 aacaggtttc ctttgccgtc cgttatgtac gtttcgtaac attcgaagac ttcctgaacc
     3241 ctgcctgccg tttcggtttc tgtacccccc cctgttagat aaggggggaa ggtttgaagc
     3301 ggttgccggc ttcctgccgt ccgctggcgc gtccgtcatc acgccggcaa ccgcctttgt
     3361 catcccttgc ttatcttcca tggtgcgaat cctcaaaaac gggcaaaaaa aaagtcctgt
     3421 tacttgtaga aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata
     3481 tataaggttt taaaacgcta agaacaaaat ccgataacgg ctctgaagaa gtaagtcttt
     3541 ctttattgca caagatagca aacttccacg gcattctcgc cccacccgtg ccgtccaagc
     3601 ctttttcaaa gacatagatg gttttcttgg caatcacttc gtcagtttgg cggtcgataa
     3661 tccgtatggt cgcacctgcc actcagtgcc tgcgcagctt ggaatcgaca ttgttttcga
     3721 acgttaccgc ataacgggcg gggtgcatat ggttaagtat ttgatttagc ggaaaatctt
     3781 tacctgaata ccgaataata tcggaatggt tgggttgcag gacatccaca tagcggtatt
     3841 ccccatcgct aaaaaatcct aggaaacgag caataaaatt tacgccttcg ctggtctgta
     3901 aagccgcatt gtcccacatc gggtctctgg tttttgcatc tgccgaaacg gtacgctcag
     3961 gtactacctt caacagcata atcccttcca cattgtccgc cgtctggtaa atctttcccc
     4021 cgccgttttg cattgttcgt taaacacggc ttcggcttct ttgtattttc tgtcccactc
     4081 ttcttgtgcc tgtatttctt ctttgctcgg gcagaattgt ttgggaataa tccaaacaaa
     4141 cagcatcagg atagcggcgg cggtcaggct gcctgaaagg attttgccgg ggttttttgt
     4201 aggcaaagcg gacgagaaac caaagcaaca gcagcatggt gtcccaatag ccgattgaga
     4261 ataggatggc caaaccttct aggaaatggc gtaaatcgtt tgtggtaaac atgggtagtt
     4321 cctgtggtta aatgtgcagg ctgctttttg ccgaaccttg ccgcatctca aaagcagcct
     4381 gcgcttcagc gttgcgttac gcagtaaaat aatgaatatt tgtaacgact tgggtatttt
     4441 ttgtcaatat tcccgccttt cccttaacag ctgccgcgct ttccgttaaa attcctttac
     4501 atatttatat tgtttcctgt ttctatattg ccaaggttat acccgttatg tttttctccg
     4561 ccctgaaatc ctttctttcc cgctacatta ccgtatggcg caatgtttgg gcggtgcgcg
     4621 accagttgga accgcccaaa cgcacggcgg aagaacaggc gtttttgccc gcgcatttgg
     4681 aactgaccga tacgccggtc tctgccgctc cgaaatgggc ggcgcgtttt attatggcgt
     4741 ttgcgctttt ggctttgttg tggtcctggt tcggcaaaat cgatattgtg gcggcggctt
     4801 cgggcaaaac ggtgtcgggc gggcgcagca aaaccatcca gccgctggaa acggtggtgg
     4861 ttaaggcggt acatgtgcgc gacgggcagc atgtgaaaca gggagaaacg ctggcggaac
     4921 tggaggctgt gggaacagac agcgatgtgg tgcagtcgga gcaggctttg caggctgccc
     4981 aattgtccaa actgcgttat gaagcggtat tggcggcatt ggaaagccgt accgtgccgc
     5041 atatcgatat ggcgcaagca cggtctttag gtctctccga tgccgatgtg caatcggcgc
     5101 aggtgttggc gcagcaccag tatcaggcat gggcggcgca ggatgcgcaa ttgcagtcgg
     5161 ctttgcgcgg ccatcaggcg gaattgcagt cggccaaggc gcaggagcag aagctggttt
     5221 cggtgggggc gatcgagcag cagaaaacag cagactaccg ccgtttgcgg gccgacaatt
     5281 ttatttcgga acatgcgttt ttggagcagc agagcaaatc ggtcagcaat tggaacgatt
     5341 tggaaagtac gcgcggtcag atgaggcaga ttcaggcggc cattgcacag gcggagcaga
     5401 atcgggtgct gaatacgcag aacctgaaac gcgatacgct ggatgcgctg cgccaggcaa
     5461 acgaacagat tgaccaatac cgcggccaaa cggataaggc aaagcagcgg cagcagctga
     5521 tgacaataca gtcgcctgcg gacggcacgg tgcaggaatt ggccacctat acggtgggcg
     5581 gtgtggtgca ggctgcccaa aaaatgatgg tggttgcgcc cgatgacgac aaaatggacg
     5641 tggaagtttt ggtattgaac aaagacatcg gttttgtgga acagggacag gatgcggtgg
     5701 tgaagattga gagttttccc tatacgcgct acggttatct gacgggcaag gtgaaaagtg
     5761 tcagccatga tgcggtaagc cacgaacagt tgggcttggt ttatacggcg gtggtgtcgc
     5821 tggacaaaca taccttgaat attgacggca aagcagtgaa tctgacggcg ggcatgaatg
     5881 tcacggcgga gattaaaacg ggtaaacggc gggtgctgga ttatctgtta agcccgctgc
     5941 aaaccaaatt ggacgaaagc tttagggagc gataggcgga tccgtactgg gcatttgtta
     6001 tccgccggtt cggacatgca gactgcctga aaccattgcc cggatgacat tgctcaatct
     6061 aatgataatg caagattacg gtatttccgt ttgcctgaca ctgacgccct atttgcaaca
     6121 tgaactattt tcggctatga aatcctattt ttccaaatat atcctacccg tttcactttt
     6181 taccttgcca ctatcccttt ccccatccgt ttcggctttt acgctgcctg aagcatggcg
     6241 ggcggcgcag caacattcgg ctgattttca agcgtcccat taccagcgtg atgcagtgcg
     6301 cgcacggcaa caacaagcca aggccgcatt ccttccccat gtatccgcca atgccagcta
     6361 ccagcgccag ccgccatcga tttcttccac ccgcgaaaca cagggatgga gcgtgcaggt
     6421 gggacaaacc ttatttgacg ctgccaaatt tgcacaatac cgccaaagca ggttcgatac
     6481 gcaggctgca gaacagcgtt tcgatgcggc acgcgaagaa ttgctgttga aagttgccga
     6541 aagttatttc aacgttttac tcagccgaga caccgttgcc gcccatgcgg cggaaaaaga
     6601 ggcttatgcc cagcaggtaa ggcaggcgca ggctttattc aataaaggtg ctgccaccgc
     6661 gctggatatt cacgaagcca aagccggtta cgacaatgcc ctggcccaag aaatcgccgt
     6721 attggctgag aaacaaacct atgaaaacca gttgaacgac tacaccggcc tggacagcaa
     6781 acaaatcgag gccatagata ccgccaacct gttggcacgc tatctgccca agctggaacg
     6841 ttacagtctg gatgaatggc agcgcattgc cttatccaac aatcatgaat accggatgca
     6901 gcagcttgcc ctgcaaagca gcggacaggc gcttcgggca gcacagaaca gccgctatcc
     6961 caccgtttct gcccatgtcg gctatcagaa taacctctac acttcatctg cgcagaataa
     7021 tgactaccac tatcggggca aagggatgag cgtcggcgta cagttgaatt tgccgcttta
     7081 taccggcgga gaattgtcgg gcaaaatcca tgaagccgaa gcgcaatacg gggctgccga
     7141 agcacagctg accgcaaccg agcggcacat caaactcgcc gtacgccagg cttataccga
     7201 aagcggtgcg gcgcgttacc aaatcatggc gcaagaacgg gttttggaaa gcagccgttt
     7261 gaaactgaaa tcgaccgaaa ccggccaaca atacggcatc cgcaaccggc tggaagtaat
     7321 acgggcgcgg caggaagtcg cccaagcaga acagaaactg gctcaagcac ggtataaatt
     7381 catgctggct tatttgcgct tggtgaaaga gagcgggtta gggttggaaa cggtatttgc
     7441 ggaataaagc aggctgaaac ggttatgaaa ttcccaaagc agcctgcacc ccgtttcgaa
     7501 agtgcaggct gctttgggat tgatccgata ctttcacatt ctcattgcct ttaaataaag
     7561 cacagttaaa aacacattta aattaagtgc gaattaattc aaatgtatat tcaattaaaa
     7621 tcaaaatagg acagtagtgc atcgtcaaat cgggcgtaat cagacaaaac ggttctcaga
     7681 taccgcttaa tattcgccca cactttctca atcgggttga gctcaggt
//
LOCUS       DAWKQB010000098         7661 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.098, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000098 DAWKQB010000000
VERSION     DAWKQB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.098"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            322..456
                     /locus_tag="WJL14_001525"
                     /pseudo
     CDS             322..456
                     /locus_tag="WJL14_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            411..626
                     /locus_tag="WJL14_001526"
     CDS             411..626
                     /locus_tag="WJL14_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7656578.1"
                     /translation="MFLFCIAMLVIGIPMILVENVIGRRKGVNALDAFGGPMNGKPIA
                     KIWKLVGWMGCSARSASWLITWYSAAG"
     gene            829..1637
                     /locus_tag="WJL14_001527"
                     /pseudo
     CDS             829..1637
                     /locus_tag="WJL14_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            1881..2537
                     /locus_tag="WJL14_001528"
     CDS             1881..2537
                     /locus_tag="WJL14_001528"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ7656579.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSDSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            2692..4377
                     /gene="rpsA"
                     /locus_tag="WJL14_001529"
     CDS             2692..4377
                     /gene="rpsA"
                     /locus_tag="WJL14_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ7656580.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            4388..4702
                     /locus_tag="WJL14_001530"
     CDS             4388..4702
                     /locus_tag="WJL14_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ7656581.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(5052..5459)
                     /locus_tag="WJL14_001531"
     CDS             complement(5052..5459)
                     /locus_tag="WJL14_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ7656582.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            5607..6716
                     /locus_tag="WJL14_001532"
     CDS             5607..6716
                     /locus_tag="WJL14_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ7656583.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            6725..7552
                     /gene="fghA"
                     /locus_tag="WJL14_001533"
     CDS             6725..7552
                     /gene="fghA"
                     /locus_tag="WJL14_001533"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ7656584.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         2039 a         1870 c         1835 g         1917 t
ORIGIN      
        1 ttcaaattcc ctctctttca tcattattta aatgctatca ttaatataat taacaaaatt
       61 tcatttccat acaccgatgt agaagaataa caaattaatc tatttataaa ctccgattct
      121 tcctcatcgg gttatactca tcagaaatac tcactgaaat atatgccttt tacatggaaa
      181 atcactagac agttgaaaag caatcagtca gttcctgtta aaaaacgcgt gtagaatttc
      241 cgctttatct caatcggaca cggtttcaat atgtcttccc cttcaaatac caatcgtcaa
      301 acctggtcca gccgattaac ctatatcctg accgttgccg gcgcgactgt cggtttcggc
      361 gcgacgtggc gtttcccgta tttggtcggt gaaaacggtg gtgcgcgtat gtgtttttat
      421 tctgtatcgc gatgctggtt atcggcatcc cgatgatttt ggtggaaaat gtcatcggac
      481 ggcgcaaagg cgtgaacgcg ctggatgcgt tcggcggccc gatgaacggc aaacccattg
      541 ccaaaatttg gaaactggtc ggctggatgg gctgctcggc gcgttcggca tcatggctta
      601 ttacatggta ctcggcggct gggtaatcag ctatatcgtt aatattattg gaggaaattt
      661 gaatatttcc agccccgtcg acggtgtggt tacaaaaggc ttctttgccg aacacattga
      721 aacagccctt gggaaattgc gttttatacg ctgctttttg tcgccgtgaa ccaatggatt
      781 ttggtcaaag gcgttatcgg cggcattgaa aaagcggcaa aatagctgat gccgctgctg
      841 tttttgttcc taatcgcgat ggtcgtccgc aacgttaccc ttccgggcgc aatggaaggg
      901 gttgctttct atctgaaacc tgatttcagc aagattaccg ccgaactgtt cgtcttcgtt
      961 ttggggcagg tattttttgc cctgagcttg ggtttcggcg tgatgattac cttgtccagc
     1021 tatttggata aaaacgaaaa tctggttcag acggcagtta tcacggcaat taccaatacc
     1081 atcatcgcca tacttgcggg ctttatgatt ttcccgtcgc tcttcagctt cggcgttgcc
     1141 cccgattccg gcccgacttt ggtgttccaa agcttgccga ttgtgttctc acatatgtgg
     1201 gcgggatctg tgttcgccgt gattttcttc tcgctgctcc tgattgccgc gttgacaact
     1261 tcgctgacca tttatgaagt gttgattacg accattcagg aaaaaaccaa aatccgccgt
     1321 accgccgcga ttacgattgt attggctgcc atcttcattt tcggcaacat cccgtccatt
     1381 ctgagctatg gtccgtggaa agacgttccg tgttcggcaa aaatattttc gatgccttcg
     1441 actacatcag cggcaacatc ttgtttatgc tgaccgcgct cggttccgcg ctgtttgccg
     1501 gttttgtgat gaaggacgaa gcgaaggacg aattgcttta taaaggcaac catacgacgg
     1561 tcaatatttg gtttgcttat gtgaaatatc ttgtgccgct ggtgattctg ctgattttcg
     1621 tcagcaacct gttctaatcc gcagcaatcg atgccgtctg aaggtcatac ctctttcaga
     1681 cagcattgcc ttgatgcccg ccacaatacc cgcaacgccg aaatccgaac cggcttgccg
     1741 acttctgtcg gatgtttccg ggcaggcggc gttccgtctg cttagacaat cgtcctttaa
     1801 aacaggtaga atccgcccca atgggaaata catccttcag acggcaaaac ccatacccca
     1861 aaccatcagg aatccccctt atgaacagac aaaaagtcat cgccatcgac ggcccgggcg
     1921 catcgggcaa aggcacggtt gccgcccgcg ttgccgccgc attgggatac gattatctcg
     1981 ataccggcgc actctaccgc ctgaccgccc tatatgcaca aaaacaaggc gtgggatggc
     2041 acgatgaaga aaacgtttcc gaactggcaa aaaaactgcc cgccgtattt tcagacagcc
     2101 gcatcctgct cggcggcgaa gacgtttcag acggcatccg gacagaagcc atcggcatgg
     2161 gcgcatccgc agtcgcacag ttgcctaaag tccgcgcctc cctgctgcaa cgccaacgcg
     2221 attttctgac cgaaaaagga ctggtcgccg acggacggga caccggatcg gtcgtcttcc
     2281 cccaagccga acttaaaatc ttcctgacgg cagaatccaa aatccgtgcc gaacgccgcg
     2341 ccaaacaaat cggcatcccc tgcgaaggtt tggcattcga gcgcatcctg tccgacatcg
     2401 aagccagaga cgaggcagac cgaaaccgca aggttgcccc cctgaaacaa caacccgatg
     2461 ccctgctttt ggatacgagc cgcctgacta tagaagaaac tgtaaaaaaa gtgcttgatt
     2521 ggtatcgtaa agtttaaatt ttcaggtata atcgcccgca ttgcgtttca gacagcatga
     2581 atttccccat atgccgtctg aaaccttatt tacccgtctg ccctgccaag gacggcagac
     2641 atccaccaac ccaccccgca ccccttggcg gtgtaccgaa aagagttata tatgtctatg
     2701 gaaaattttg ctcagctgtt ggaagaaagc tttaccctgc aagaaatgaa cccgggtgag
     2761 gtgattaccg ctgaagtagt ggcaatcgac caaaacttcg ttaccgtaaa cgcaggtctg
     2821 aaatcagaat ccctgattga tgtagctgaa ttcaaaaacg ctcaaggcga aattgaagtt
     2881 aaagtcggcg acttcgttac cgttaccatc gaatccgtcg aaaacggctt cggcgaaacc
     2941 aaactgtccc gcgaaaaagc caaacgcgct gccgattgga ttgccctgga agaagccatg
     3001 gaaaacggca acatcctgtc cggcatcatc aacggcaaag tcaaaggcgg cctgaccgtt
     3061 atgattagca gcatccgcgc attcctgcca ggttctttgg tcgacgtacg tcctgtaaaa
     3121 gacacttctc acttcgaagg caaagagatc gaattcaaag tgattaaact ggacaaaaaa
     3181 cgcaacaacg tcgttgtttc ccgccgcgcc gttctggaag ccactttggg tgaagaacgc
     3241 aaagccctgc tggaaaacct gcaagaaggc tccgtcatca aaggcatcgt taaaaacatt
     3301 accgattacg gtgcattcgt tgacttgggc ggcatcgacg gtctgttgca catcaccgat
     3361 ttggcatggc ggcgcgtgaa acacccgagc gaagtcttgg aagtcggtca ggaagttgaa
     3421 gccaaagtat tgaaattcga ccaagaaaaa caacgcgttt ccttgggtat gaaacaactg
     3481 ggcgaagatc cttggagcgg tctgacccgc cgttatccgc aaggcacccg cctgttcggc
     3541 aaagtatcca acctgaccga ctacggcgca ttcgtcgaaa tcgaacaagg catcgaaggt
     3601 ttggtacacg tctccgaaat ggactggacc aacaaaaacg tacacccgag caaagtcgta
     3661 caactgggcg acgaagtcga agtcatgatt ttggaaatcg acgaaggccg ccgccgtatc
     3721 tctttgggta tgaaacaatg ccaagccaat ccttgggaag aatttgccgc caaccacaac
     3781 aaaggcgaca aaatctccgg tgcggttaaa tccattaccg atttcggcgt attcgtcggc
     3841 ctgcccggcg gcatcgacgg tctggttcac ctgtccgacc tgtcctggac cgaatccggc
     3901 gaagaagccg tacgcaaata caaaaaaggc gaagaagtcg aagccgtcgt attggcaatc
     3961 gacgtggaaa aagaacgcat ctccttgggt atcaaacaac tggaaggcga tccgttcggc
     4021 aacttcatca gcgtgaacga caaaggttct ttggttaaag gttccgtgaa atctgttgac
     4081 gccaaaggtg ctgttatcgc cctgtctgac gaagtagaag gctacctgcc tgcttccgaa
     4141 tttgcagccg accgcgttga agatttgacc accaaactga aagaaggcga cgaagttgaa
     4201 gccgtcatcg ttaccgttga ccgcaaaaac cgcagcatca aactttccgt taaagccaaa
     4261 gatgccaaag aaagccgcga agcactgaac tccgtcaatg ccgccgccaa tgcgaatgcc
     4321 ggcaccacca gcttgggcga cctgctgaaa gccaaactct ccggcgaaca agaataaggt
     4381 tgcagacatg acaaagtctg agttaatggt tcgtttggca gaagtgtttg ccgccaaaaa
     4441 cggcacgcat cttctggcaa aagacgtaga gtacagcgta aaagtcttgg ttgacaccat
     4501 gactagatcg cttgcccgag gtcaacgcat cgaaatccgc ggtttcggca gcttcgattt
     4561 gaaccatcgt cctgcccgca tcggtcgcaa tcccaaaacc ggcgagcgtg tggaagtacc
     4621 tgaaaaacat gtaccccact tcaagcccgg taaagaattg cgcgagcggg tcgacttggc
     4681 tttaaaagaa aatgccaatt aaaccttagc atcaaaacgc cgctgttacg cggcgttttt
     4741 tctgtggttt aacttcatcc gttgcttcaa taccttgagc caagcaagca acggattaga
     4801 gcgtggattt tttatagtgg attaacaaaa accagtacgg cgttgcctcg ccttagctca
     4861 aagagaacaa ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttacac
     4921 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatatca ttgcttacaa
     4981 tccgcttttt aaacaacaaa tttttgattt ctattacgaa caggacaaaa atcctgctta
     5041 ttgcactaaa actaagccgt ttcaggaatt tgcggcaaat ttacagcttt taccgagcct
     5101 aatgctttcg ctttttggta aaacgccaat ttgtattcaa gcaaatctaa atagcgtttt
     5161 aattcggcaa tttgacactt cacattttct atttgatttt caaacaagga aaggcgttct
     5221 tcaatggtat cgtcgccaat gacggtacat tccgcaaagc gtttgatgtc ttttaagctc
     5281 attcccgtat ttttcaagca ttgcaataag cccaaccatt gcaaatcgtt atcggtaaaa
     5341 cagcggttac cgtattcatc acgtccgata ttgggcaaca aaccttcttt gtcgtaaaaa
     5401 cgtagggtgt gggcggagat gcctattttt tcggcggctt tggcagtagt ataagtcatt
     5461 ttccctcctt ctaaacaaaa acagtaaaaa acacttgctt tagagttaac tctaaagtgt
     5521 aaactgttgc tatgttgctc aggcaaggca actttgtcaa tgaattaaga ggaaagacaa
     5581 tggaaatgaa acaagccgat tcaaccatca aatctcgtgc ggcggtggca ttcgccccta
     5641 accaaccctt acaaattgtg gaaatcgacg tagaaatgcc gcgtaaaggc gaggtgttaa
     5701 tccgcaatac ccacactggc gtgtgccata ctgatgcgtt tacgttatca ggaagcgatc
     5761 ctgaaggcgt attccctgtg gtgcttggac acgaaggtgc gggtgtggtc gttgctgtgg
     5821 gcgaaggtgt gtcaagcgta aaaccgggtg atcacgtgat tccgctttac accgccgaat
     5881 gtggcgaatg tgagttttgt tgttcaggta aaaccaactt gtgcgtctca gtgcgtgata
     5941 cacaaggtaa aggcttaatg ccggacggca cgacgcgttt ttcttatcaa ggtcagccga
     6001 tttatcacta tatgggctgt tcgactttca gtgaatactc cgttgttgcc gaagtttcac
     6061 tggcgaaaat caaccctgaa gccaaccatg aacaagtatg tttgctcggc tgcggcgtta
     6121 ccacaggtat tggtgcggta cataatacgg caaaagtgca agaaggcgac tctgttgccg
     6181 tgtttggttt gggggcgatt ggtttggcgg tggtgcaagg tgcgcgtcaa gccaaagccg
     6241 gccgcattat cgccattgat accaatccat caaaatttga gttggcaaaa cagttcggtg
     6301 caacggattg tttgaacccg aacgattacg ataaaccaat taaagatgtg ttgttagaca
     6361 tcaacaaatg gggcattgac catacctttg aatgtatcgg caatgtaaac gtaatgcgtc
     6421 aagcattaga aagtgcacat cgtggttggg gtcaatccat tatcatcggc gtagcaggtg
     6481 caggacaaga aatttcaacg cgtccgttcc agttggtaac aggtcgtgtt tggaaaggtt
     6541 cagcatttgg cggtgtgaaa ggtcgctctg aactgccgaa aatggtggaa gattcaatga
     6601 aaggcgacat tgagttagaa ccgtttgtaa cccacacaat gacactcgat caaatcaata
     6661 aagcctttga cttaatgcac gaaggtaaat cgatccgcgc cgttattcac tactaaggta
     6721 tgcgatgaaa ctgattgaac aacatcaaat ttttggcggt tcgcaacaag tttgggcgca
     6781 tcatgcccaa acgctgcaat gcgaaatgaa atttgccgtc tatttgccaa ataatccaga
     6841 aaatcgaccg cttggtgtga tttattggct ttccggcttg acgtgtacgg aacaaaattt
     6901 cattaccaaa tcaggctttc agcgttatgc ggcagaacat caagtaattg tggtggcctc
     6961 cgataccagc cctcgcggag agcaagtgcc gaacgatgat gcttacgatt taggacaggg
     7021 tgcaggcttt tatttgaatg cgaccgaaca gccttgggcg gcgaattatc aaatgtatga
     7081 ttacattttg aacgagctgc cccgtctgat tgagaaacac tttcctacca acggcaaacg
     7141 ttccattatg gggcattcaa tgggcggaca cggcgcattg gtattggcgc tgcggaatca
     7201 ggaacgttat caaagtgttt ctgccttttc gcctatttta tcgccaagcc tcgtgccgtg
     7261 gggagaaaaa gcctttactg cttatttagg gaaagaccgt gaaaaatggc agcaatatga
     7321 tgcgaactca ctcattcaac aaggctataa agtgcaaggt atgcgcatcg atcaaggctt
     7381 ggaagatgag tttttgccga cacaattgcg taccgaagat tttatcgaaa cctgccgtgc
     7441 ggcaaaccag ccggtcgatg tgcgtttcca taaaggctac gatcacagct attacttcat
     7501 cgccagtttt attggcgagc atattgctta tcacgccgcg tttttgaagt aaaccaaaga
     7561 gcgttcagtg ttcaaagcag ttttgggata gccggcacga gggcggtaag aagtgccggc
     7621 ataaacgtat gccgtctgag ccggaagaag ccgactctac g
//
LOCUS       DAWKQB010000099         7659 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.099, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000099 DAWKQB010000000
VERSION     DAWKQB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.099"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            33..1703
                     /locus_tag="WJL14_001534"
     CDS             33..1703
                     /locus_tag="WJL14_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245501.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ7656585.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLFIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLVDRFHNTGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGEIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKDVKYDTRINTAVPQVNPIDEPVF
                     NPKGSVGSAHSWSITARIQYAKLPRQGRIRYIPPKNYSPSAPLPKGPNNGYLDKFGNE
                     WTKGPSRTKGQEFEWDVQLSKTGREQLGWASRDGKHLNISIDGKITHK"
     gene            1700..1942
                     /locus_tag="WJL14_001535"
     CDS             1700..1942
                     /locus_tag="WJL14_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ7656586.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2020..2265
                     /locus_tag="WJL14_001536"
     CDS             2020..2265
                     /locus_tag="WJL14_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7656587.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            2268..2657
                     /locus_tag="WJL14_001537"
     CDS             2268..2657
                     /locus_tag="WJL14_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656588.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            2684..2833
                     /locus_tag="WJL14_001538"
     CDS             2684..2833
                     /locus_tag="WJL14_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ7656589.1"
                     /translation="MRLSDGIFDIKRITKRAFDSKGTSEIPDYTWHHHQDTGCIGWKA
                     MNKRR"
     gene            2836..3222
                     /locus_tag="WJL14_001539"
     CDS             2836..3222
                     /locus_tag="WJL14_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656590.1"
                     /translation="MWKIYKENSTDLNFSLGSIYCQAINITEFKMWVEKIIREMDLDE
                     IPNYFFDLTDLQSLFHLIDIIGFVPENNLSKNQDNALTGIAFLRGIDVYDPPVSKEKA
                     LKALEKHPEIYQKFRHFFPFVELPPL"
     gene            3370..3759
                     /locus_tag="WJL14_001540"
     CDS             3370..3759
                     /locus_tag="WJL14_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656591.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKHPEIYQRFQHFFPFVELPLL"
     gene            complement(3800..5257)
                     /locus_tag="WJL14_001541"
     CDS             complement(3800..5257)
                     /locus_tag="WJL14_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ7656592.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILTAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(5272..6102)
                     /locus_tag="WJL14_001542"
     CDS             complement(5272..6102)
                     /locus_tag="WJL14_001542"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ7656593.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGYGEGAPKRSDTIEPILKHPDGRKMLDWL
                     RAQPLLIREGNRVMVHAGILPQWRITKAESLAGEAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPLYLRPWFKAPDRQNLD
                     HTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFTK
                     "
     gene            complement(6180..6569)
                     /locus_tag="WJL14_001543"
     CDS             complement(6180..6569)
                     /locus_tag="WJL14_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ7656594.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFKNLQAVAEAAGGTLADIVKLNAYLTDLANFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(6705..7229)
                     /gene="nspA"
                     /locus_tag="WJL14_001544"
     CDS             complement(6705..7229)
                     /gene="nspA"
                     /locus_tag="WJL14_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ7656595.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGVRVKF"
     gene            complement(7336..7596)
                     /locus_tag="WJL14_001545"
     CDS             complement(7336..7596)
                     /locus_tag="WJL14_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656596.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKNRMPAPATIRQIP
                     LFFPNRLQVSPIRRVMPPPPFKGNARVNGFAVGKAAGYRYVS"
BASE COUNT         1998 a         1659 c         2134 g         1868 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggaa cttgccgagc gcagcggtca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgttcatcc agcaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg attcagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgccg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgtc gaccgtttcc
      601 acaataccgg tagtatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcgggcaatg ccgccgaagc tttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aggctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccgtca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgag atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gaaagaatgt gaaactggca aacaaacgcc
     1261 acccgaagac caaagtgccg tttgacggta aagggtttcc gaattttgaa aaagacgtaa
     1321 aatacgatac gagaattaat accgctgtac cacaagtgaa tcctatagat gaacccgtct
     1381 ttaatcctaa aggttctgtc ggatcggctc attcttggtc tataactgcc agaattcaat
     1441 acgcaaaatt accaaggcaa ggtagaatca gatatatccc acctaaaaat tactctcctt
     1501 cagcaccgct accaaaagga cctaataatg gatatttgga taaatttggt aatgaatgga
     1561 ctaaaggtcc atcaagaact aaaggtcaag aatttgaatg ggatgttcaa ttgtctaaaa
     1621 caggaagaga gcaacttgga tgggctagta gggatggtaa gcatttaaat atatcaattg
     1681 atggaaagat tacacacaaa tgactaattt aaaattagat ttctactctg aagttattat
     1741 aaaagattct tgccctaatg atttgttaga aaatggtgag actattaaag gaaaaaaagg
     1801 agtggtatta ggtataagcg aagaagatgg tataatctat ggctatacta ttttactttt
     1861 tgatataaaa tattgtatat atatagataa gaaatatatt ataccgactg gaaaaaaatt
     1921 ctctcgcgat gatttttatt agctaattta agtaatttaa gcaagttttt tagatactga
     1981 aactggtaat tatgtttatg taagcataag gagttcagat tgaaggattc tacagggcaa
     2041 atgaggctgg caaccaagga tttggcggaa gccattaaac gaggagaagt acgtagttct
     2101 gcttttacaa caaagcaact aaaggcaatc gaaaaaggag aatctaaaat accggattac
     2161 acttggcatc atcatcaaga cactggaagg atgcaattga ttaatgaaga cttgcatcat
     2221 gataccggcc atatcggttg gagagcgatg agtaaaggaa agtaactatg tggaaaatca
     2281 taaaagagga tagtgatgat ttaggatttg caattaaatg cttattctct caatctattg
     2341 atttaaatga attcaagtta tggattgaac aagtaatacg cgatatgccc atcgaggaca
     2401 tcccttttta tatttttgac ttggcggatt tcgatgtagg aattggcgat attggcaata
     2461 ttgtaggttt tgctccgagt tccagcctgc cgaaatcgaa aaaaaatgcc ttgaccggca
     2521 ttgccttctt aagggggata gatgtctatg accctcccgt ttcaaaagaa aaagcattaa
     2581 aagccttaga gaaatatcct gaaatttatc agaaatttca gcatttcttt ccgtttgtag
     2641 agctgcctcc gctttaaaca gtcaaaatgc cgtctgaaac aatattcggc tttcagacgg
     2701 catttttgat ataaagcgga taactaaaag agcgtttgac agcaaaggaa catctgaaat
     2761 accggattac acttggcatc atcatcaaga tacaggatgt attggttgga aagcgatgaa
     2821 caaaagaaga taattatgtg gaaaatttat aaagaaaata gcactgactt aaatttttcc
     2881 ctaggtagta tatattgcca agcaatcaat attaccgaat ttaaaatgtg ggtcgaaaaa
     2941 atcataaggg aaatggactt agatgaaatt ccaaattatt tttttgatct aacagatctt
     3001 caatcactat tccatctaat tgatattata ggatttgttc ccgaaaataa cttatcaaaa
     3061 aatcaggata atgcattaac aggcattgcc ttcttaaggg ggatagatgt ctatgaccct
     3121 cccgtttcaa aagaaaaagc attaaaagcc ttagagaaac atcctgaaat ttatcagaag
     3181 tttcggcatt tctttccgtt tgtagaactg cccccgcttt aaacaatcaa aatgccgtct
     3241 gaaacaatat tcggctttca gacggcattt ttgatacaaa gcaggtaacc aaaagagcgt
     3301 ttgacggcaa aggagaatct aaaataccgg atgtatcggt tgggaagcaa tggataaagg
     3361 taaataatta tgtggaaaat tagtaaagaa aattgtgaag atttaggatt tgcaatagtc
     3421 tgtatgttct atgatgctat taatctttct gaatttaaat tatggttgga tatagttgtc
     3481 agagatactc ctattgatac aattccattg tatatttttg atttgattga ttttgataag
     3541 agtatagggg aaatttatga tgtaattgga gtcgttaatt atggttacat ttcaaatgat
     3601 caaaaaaatg cattaacggg cattgccttc ttaaggggga tagatgtcta tgatccgcct
     3661 atttcaaaag aaaaagcatt aaaagcctta gagaaacatc ctgaaattta tcagaggttt
     3721 cagcatttct ttccttttgt agagcttccg cttctttaaa ggacaagatg ccgtctgaaa
     3781 agttttcaga cggcattttt tatttcttcc agtaggcggg ggtgaagagg atgaagacgg
     3841 tgaagatttc cagcctgccc aagagcatgg cggtaacgca gatccatttc tgcatcacgt
     3901 ccaaaccggc gtaattgccg gcgggcccga cttcgcccag gccggggccg gcgttggtga
     3961 tgcaggcgat gacggcggtg aaggcggtgg taaattccat accgctcgcc atcagcagga
     4021 agctgaagag gacgacggtc ataaagtaga tgaagatgaa ggacataacg gtcagcgcga
     4081 ggcggtcggg tatggccttg ccgctgattt tgacggtgcg gacggctttg gggtgcagca
     4141 gcaccatcat ttcgcgcagg ctgaatttga acaggacgag ggcgcgtatg gttttgatgc
     4201 cgccgccggt cgagccggag ttggcgagga tgttggcgag gaaaaacatc cacagggaaa
     4261 tcaggagcgg ccattgtgcg aagtcggtgt tggccagccc gtttgccagt ccgatggaga
     4321 cgaagttgaa ggcggtgtag cgcagggatt cggtaaaacc ggcgtaatag ccggtgtgcc
     4381 acaggtacag ggcggcggta aggatgctgc cggagagcag cagcagcatc gtccggcatt
     4441 cttcgtcttt ccaataggtt ttgaggctgc ggctgttgag ggcggcgaaa tggctggcaa
     4501 aattgatgcc gccgacaatg gtgaaaacga tgatgaccgc ctcgatgagg ggggagttgt
     4561 aataagctat gctggcatcg tgggtggaaa atccgcccag cgagagggtc gccattgcgt
     4621 gacagacggc atcgaaccag ctcatcccgg caaaatgcag gcaggctgcc gcgaggatgg
     4681 tgatcagggt gtagccgaac cagagttttt tcgccacttg ggaaatgcgc ggcgacattt
     4741 tgctttcttt gtcaatgccg gggatttcgg ctttgaataa ctgcgtgccg cctacgccga
     4801 gcataggcag gatggcgacg gcaaggacga tgatgcccat cccacccagc cagttgagca
     4861 tatgccgcca aaagttgacg gagggggcga gcccgtcgac gtgggggatg acggtcgcgc
     4921 cggtggtggt cagtcccgac atcgattcaa aaaatgcgtc ggtaaagccc atattcggga
     4981 aatacaggta catcggcatc gccgccatag cggcaaacgc cagccacaac atcaggacga
     5041 gggtgaagcc gtcgcgcggg cgcagttcgc gcctgaaccg gagggtggcg agccggacga
     5101 tgtacgagcc ggaaagggta acggtcgcgg tggtggcgaa ggcggtgtac gcgccgtccg
     5161 aaaaggcgta ggagagggcg gcgggtatca gcaggataaa ggaaaacagc atacccagtc
     5221 gggagaggac gtgggcgatg ggcaggattt tgtgcatagt ggggcggtcc gttattttgt
     5281 gaagcttttc cagtctatgc cgccggcggc ttggacttgg gtaatttctt ccgtttcgag
     5341 attgacggcg gtcagctgtc cgccccacag tgcgccggtg tccagcgaga tgacgttgtc
     5401 ggcattcgtg tagcccaagg aagaccagtg tccgaagatg atggtgtggt cgaggttttg
     5461 ccggtcgggg gctttgaacc acgggcgcag gtaaagcggc atttttttca ctgtggattt
     5521 gtagtcgaaa tccagttcgt ttttaaaggt cagggcgcgc atccgcgtga aggcgttgac
     5581 gatgaagcgc aggcgggcat agccttccaa accttcgtcc catgcggccg gtttgttgcc
     5641 gtacattttg gagaagaatt tgacgtattt tttgccgcgc agttcggctt cggcttctcc
     5701 ggcgagcgat tcggctttgg ttatgcgcca ttgcggcagg atgccggcgt gtaccatcac
     5761 gcggttgccc tcgcgtatca acagcggttg cgcgcgcagc cagtcgagca tttttctgcc
     5821 gtcggggtgt ttgagtatgg gttcgattgt gtcgctgcgt ttgggcgcgc cttcgccgta
     5881 gccgacggca agcaggtgca ggtcgtggtt gccgaggacg atttgcacgc tgttttcgtg
     5941 ccggatgcag aattgcagcg tttcgaggga tttcgggccg cggttgacga tgtcgcccgt
     6001 cagccagagg gtgtctgtgc cgtggttgaa gccgattttg ccgagcagcg cggtcagttc
     6061 gtcgaaacag ccttggatgt cgccgattgc gtaatgtgcc attgcagatg ttgtgaagtg
     6121 ggaaagtgtt gcggttcgga cggcacggtc ttgaaatatc atgccgtctg aacgtggaat
     6181 tatgcgttca aaacgaggac ggcttcggct tcgacctgca cgcctttggg cagcgaggca
     6241 acgccgacgg cggcgcgggc ggggaagggc tcggcaataa attccgccat cacttcgttg
     6301 aagacggcga agttggcaag gtcggtcagg taggcgttga gcttgacgat gtcggccagc
     6361 gtgccgcctg cagcttcggc gacggcctgc aggtttttaa agacctggcg cgcttcggcg
     6421 cggaaatcgc cgttgccgac aacggtcatc gtggcgggat cgagggggat ttgaccgctc
     6481 atgtaaacgg tgtcgcctgc tcggacggct tggctgtatg cgccgatggc ggcaggggct
     6541 ttgtcggtgt ggatgatggt tttggacatt tcggattcct caaaaaatag ggcggcagaa
     6601 gccgcagcat tcgggattat cgtacaaaac cgccggcttg tgtagttgcg gtggcagaaa
     6661 acaaaaccgc cgaaggctcg gcggtttgca gaataaggcg catatcagaa tttgacgcgc
     6721 acaccggcgg acagttcgcc ggaacggacg tttttgacag tgttgacttt gccgatgtag
     6781 ttgtagcggt agccggcatc caaatcgaca ttcggggtaa cggcatagct tacgcccgcc
     6841 aatacgccga ggccggtgga ggtttggctg aagctgtcgc tgccgcccaa gtcgacggag
     6901 gcgcggttga ggctcaagcg cgcgccgaga tacggtttga cgggcgattg ggtgtcgaag
     6961 tcgtaaatgg cggacgcgcc gatgctgtaa agtttgaaat cggtggatgg ggctttatag
     7021 tttttgtagc gcgtgtaatc gacggcgaag cggaggtcgt tgatgcggta gcctgcggag
     7081 atgcgcgggc tgaagccttt ggcagaacct aaagagcttg aggcttttgc gtgtgcggca
     7141 tcggcttgga cgtaaaagcc ggatgcgcct tccgccagtg cggcggccgg gagagcgagg
     7201 gcaatcagtg tggcaagtgc ttttttcata ttttggttcc tttatggtca gttagaaaaa
     7261 ttgttaagaa tccgttaaag aattctgctt tattatactt aaattttctt tttgcatcgt
     7321 aatattttta atacttcaag atacgtagcg gtatccggct gctttgccga cggcaaagcc
     7381 gttaacccgc gcgttgcctt taaatggtgg tggcggcatc acgcggcgga tgggtgaaac
     7441 ttgcaaacgg tttggaaaaa acagcggtat ctgtcggatt gttgcaggtg caggcatacg
     7501 gttttttgtg tgcgtctgtg ccttaagcgt cggacatttc cggcggcggc tgtgccgtct
     7561 gaaacgcccg gcgggggatg cggctgcgtt ttccatcgat aagcatattt tccggacgcg
     7621 ttcggggggg ttttcccggg cggccgccga tttgtttgc
//
LOCUS       DAWKQB010000100         7628 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.100, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000100 DAWKQB010000000
VERSION     DAWKQB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.100"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            8..1876
                     /locus_tag="WJL14_001546"
     CDS             8..1876
                     /locus_tag="WJL14_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ7656597.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKDWLPGGFLGVDIFFV
                     ISGFLMTTILHREMSGGGRFSLKAFYIRRIKRILPAFFAVLAATLAGGFFLFTKDDFF
                     LLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLVA
                     RKSLRVQFGFLAALCALSLAASFIPSALDKYYLPHLRACELLIGSLTAVWMRCRQPAV
                     GRRCAAVGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKKF
                     FQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSSAAAIVLTLAFSLISYHCI
                     EKPFKKWQGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNNT
                     GKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWAATMVSADACAYVEGYASR
                     VFQNWAACRAVYRYAEEHLPRYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLHK
                     LSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQALRPDDESTLKANARIRELAAK
                     YPNVYIIDAAAYIPADFQIGGLPVYSDTDHINPYGGTELAKRFSEKQRFLNTRHNH"
     gene            1938..3233
                     /gene="tyrS"
                     /locus_tag="WJL14_001547"
     CDS             1938..3233
                     /gene="tyrS"
                     /locus_tag="WJL14_001547"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ7656598.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGTKPEAQRILAEEMTRLIHGEAALQAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            3532..4452
                     /gene="ribF"
                     /locus_tag="WJL14_001548"
     CDS             3532..4452
                     /gene="ribF"
                     /locus_tag="WJL14_001548"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ7656599.1"
                     /translation="MKIRLGRHNAPDFPQGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            4594..7383
                     /gene="ileS"
                     /locus_tag="WJL14_001549"
     CDS             4594..7383
                     /gene="ileS"
                     /locus_tag="WJL14_001549"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ7656600.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYSVVLARQLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
BASE COUNT         1814 a         2188 c         1932 g         1694 t
ORIGIN      
        1 aaacattatg agccaagcct taccctaccg cccggacatc gacacattgc gcgccgccgc
       61 cgtcttgtcc gtcatcgtgt tccatatcga aaaggattgg ctgccgggcg ggtttctcgg
      121 tgtcgatata ttctttgtga tttcaggctt tttgatgacg acgattcttc accgcgaaat
      181 gtcggggggg gggaggtttt ccctgaaggc attttatatc cgccgcatca agcggattct
      241 gcccgcattt ttcgccgtat tggcggcaac gctggcaggc ggcttctttt tattcaccaa
      301 agatgatttc tttcttttgt ggaaatccgc gctgaccgcc ttgggtttcg cctctaacct
      361 gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc ccctgctgca
      421 catctggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatac tgctgctgct
      481 tgtcgcccgc aaaagcctgc gcgtacagtt cggcttcctt gccgcactgt gtgccttaag
      541 ccttgccgcc tcctttatac cttccgcgct cgataaatat tacctgcccc acctgcgcgc
      601 ctgcgaatta ctgataggat cgctgaccgc cgtgtggatg cgctgccggc aacctgccgt
      661 cggcagacgc tgtgccgccg tcggcgcatt gtttgccgtg tgcatattgt caacctgcct
      721 gttttcctat tcggaacaaa ccgcctattt cccgggcccc gccgctttga ttccttgcct
      781 ggctgttgcc gcgctgattt atttcaacca ttacgaacac ccacttaaaa aatttttcca
      841 atggaaaatc accgttgccg ccggtttgat ttcctattcg ctttatctgt ggcattggcc
      901 gatattggcc tttatgcgct atatcggtcc ggacaacttg ccgccttatt cgtcggcggc
      961 agcgatcgtc ctgactctgg cgttttccct gatttcttat cactgcatcg aaaaaccgtt
     1021 taaaaaatgg caaggttcgt tcgcacaatc cgttttatgg atttatgcct tgcctatgct
     1081 cattttgggg gcgggctcgt ttttcgcgat gaggctgccg tttatggcgc aatacgaccg
     1141 cttggggctg acgcgttcca acacctcctg ccacaacaat accggcaaac aatgcctatg
     1201 gggggatacg gaaaaacagc cggaactgct ggttttgggc gactcccacg ccgaccatta
     1261 caaaacattc ttcgatgccg tgggcaaaaa agaaaaatgg gctgccacta tggtttccgc
     1321 cgacgcttgc gcctatgtgg aaggctacgc gtcccgcgta ttccaaaact gggccgcctg
     1381 ccgcgccgtt taccgctatg ccgaagaaca cctgccccgg tattcaaaag tggttttggc
     1441 gatgcgctgg ggcagccaaa tgcccgaaaa cagccgctcc cttgcctatg atgccggttt
     1501 tttccaaaaa ttcgaccgta tgctgcacaa actctcatcc gaaaaacaag ccgtttacct
     1561 gatggccgac aaccttgcct cgtcttacaa cgtacaacgc gcctatatct tgtcttcccg
     1621 cataccgggt taccgccaag ccctgcgccc ggacgacgaa agcaccctga aagccaatgc
     1681 ccgcatcagg gaattggcag ccaaataccc caacgtctat attattgatg ccgccgccta
     1741 tatccccgca gattttcaaa tcggcggatt gccggtttac tcggacacag accacatcaa
     1801 cccttacggc ggcacggaat tggcaaagcg tttttccgaa aaacaacggt ttctcaatac
     1861 gcgccataac cattgattcg cttaaatttg ttacaatcgg cggtttgcaa aaacgctaat
     1921 ttttttgaaa gagaccgatg agcgtcatcc aagacctgca atcgcgcggc ctaatcgcgc
     1981 aaacgaccga catcgaagcc ttagacgctt tgttgaacga acaaaaaatc gccctttact
     2041 gcggcttcga cccgaccgcc gacagcctgc acatcggaca cctgttgccc gtattggcat
     2101 tgcgccgctt ccaacaggcg gggcatacgc caattgcact ggtgggcggc gcgaccggta
     2161 tgattggcga ccccagcttc aaagccgccg aacgcagctt gaattccgcc gaaaccgttg
     2221 ccggctgggt agaaagcatc cgcaaccaac tgaccccttt cttaagcttt gaaggcggaa
     2281 acgccgccat tatggcgaac aacgccgact ggttcggcaa aatgaactgc ctcgacttcc
     2341 tgcgcgacat cggcaaacat ttctccgtca acgccatgct gaacaaagaa tccgtcaaac
     2401 agcgcatcga ccgcgacggc gcaggcattt ccttcaccga gttcgcctat tccctgctgc
     2461 aaggttacga cttcgccgag ttgaacaaac gccacggcgc ggttttggaa atcggcggct
     2521 ccgaccaatg gggtaacatc actgccggta tcgacctgac ccgccgtctg caccaaaaac
     2581 aagtattcgg cctgaccctg cctttggtta ccaaatcaga cggcaccaaa ttcggcaaaa
     2641 ccgaaggcgg cgcggtgtgg ctgaacgcga aaaaaacctc gccgtatcag ttctaccaat
     2701 tctggctgaa agtcgccgat gccgatgtgt ataaattcct gaaatacttc accttcctgt
     2761 ccatcgaaga aatcgacgcc atcgaagcga aagataaagc cagtggcacc aagcccgaag
     2821 cgcaacgcat cctcgccgaa gaaatgaccc gactgattca cggcgaagcc gccctacaag
     2881 ccgcacagcg catttccgaa agcctgtttg ccgaagatca aagcagcctg accgaaagcg
     2941 acttcgagca gctcgccctc gacggcctgc ctgcatttga agtttcagac ggcatcaatg
     3001 tcgtcgaagc cttggtaaaa accggtttgg catcctccaa taaagaagcg cgcggctttg
     3061 tgaacagcaa agcggttttg ctcaacggca aacctgccga agccaacaac cccaaccacg
     3121 ccgccgaacg ccccgacgat gcctgcctgc tgaacggcga acacaaacgt ttcggcaaat
     3181 acactatcct tcggcgcggc aaacgcaacc acgcgctttt ggtttggaaa taatccgatt
     3241 gccgcagaaa tgccgtcatt cccgcctgcg cgggaatgac ggcggagggt tgtttgtctc
     3301 ggtttacctg gttaaaaaag aacgattttc actgatgttg catcaggttt ggggcgatgt
     3361 ttcaacacat aggatcgcgc ctgatgcacg ctttatgcgt ttggcgcgtt cggcggcggg
     3421 aaatttgcct gcttttccag cgtcgggcgg gcgtaacggg cggcacactg tctataaacc
     3481 gcaataccgt ttacaatgac cgcctgtttc accacatacc cgaacgcaac aatgaaaatc
     3541 aggctggggc ggcacaacgc gcccgacttt ccacagggtg ccgccgtaac cataggcaat
     3601 ttcgacggcg tacacctcgg acacaaacac atcctccaaa aactccgcct cgaagccgac
     3661 gcgcgcggac tgcccgtcgt tgccgtcatc ttcgaacctc aacccaaaga attttttgcc
     3721 gccctcgcag gcaaaacccc accctgccgc atcagccccc tgcgcaccaa gctcgaatta
     3781 ttggaaggca caggctgtgt cgatgccgtt tgggttttgc gtttcgatca aaatttttcc
     3841 gaaatatccg cgcaagggtt tatcgaccgc ctgctgcgtc aaaccttgaa tacgcgttat
     3901 ttgctcgtcg gcgacgattt ccgtttcggt gcggggcggg aaggctgttt tgaacttttg
     3961 gcacaacagc ccgatatgca gaccgagcgt acgccttccg tcatcgtcga agacatccgc
     4021 accagcagta ccgccgtgcg acaagccctt tcagacggca accttgccta tgcgaaaaaa
     4081 cttttgggac acgactacgt cttgagcggc agggtggtgc acggcagaaa actcggacgc
     4141 accttaaacg ccccgactgc caacatccgc ctgccccgcc accgttatgc actcggcggc
     4201 gtgttcgtcg tcgaagcgga cggcgcattc ggcacgcggc gcggcgtggc gagcttcggc
     4261 ttcaatccca ccgttgatag cggctgttct caaaagcttg aagtccacct gttcgacttt
     4321 caaggcgacc tgtacggaca acggttgaac gtccgcttcc tgcacaaact gcgcgatgaa
     4381 gaaaagtttg acggtatggc agaactgaaa aggcagatag cagccgatat ggaagccgca
     4441 aagtgttggt agaaaaacct tatacaaacc atccgattgg gctacaatta atcttttaac
     4501 ttttcagacg gcataggggt ttcccgttgt gaaatactgt ttgaggcgca atgccgtctg
     4561 aaaccgaaat attgtaacaa tagagattaa aaaatgaccg attacagtaa aaccgtaaac
     4621 ctgctcgaga gcccgtttcc gatgcgcggc aaccttgcca agcgcgagcc tgcatggctg
     4681 aaaagctggt acgagcaaaa acgctaccaa aaactgcgcg aaatcgccaa aggccgtccg
     4741 aaattcatcc tgcacgacgg ccctccgtat gccaacggcg acatccacat cggtcatgcc
     4801 gtcaataaaa tcctcaaaga catcattatc cgcagcaaaa ctcaagccgg ttttgacgcg
     4861 ccttatgtgc cgggttggga ctgtcacggt ttgcctatcg aagtgatggt ggaaaaactg
     4921 cacggcaaag acatgcctaa agcccgtttc cgcgaattgt gccgcgaata cgccgccgaa
     4981 cagattgccc gtcagaaaaa agactttatc cgcttgggcg tgttgggtga ctgggaccat
     5041 ccttacctga ccatggattt caaaaccgaa gccgataccg tgcgtatgct cggcgaaatc
     5101 tacaaatccg gctatctcta ccggggtgcg aaaccggttc aattctgctt ggattgcggc
     5161 tcttcattgg ccgaagcgga agtggaatat aaagacaaaa tctcgcccgc gattgacgtt
     5221 gcctatccgt ttaaagacac cgccgcgctt gccgccgcat tcggtttggc aggtatcgaa
     5281 ggcaaagcgt ttgccgttat ttggacaacc acgccttgga ctttgcctgc gagccaagct
     5341 gtttcagcgg gtgcagacgt ggtgtatcaa ttaatcgata cgcccaaagg caaattggta
     5401 ttggccaaag atttggcgga agacgctttg aaacgctacg gcttttcaga cggcatttct
     5461 attcttgccg aaaccaccgg cgacaagctg gaaaacctgc acatgaacca tccgttcctc
     5521 gaacgcgata ttcccatgct taacggcgaa cacgttacca ccgatgccgg taccggcttg
     5581 gtgcacaccg cccccgcgca cggtttggaa gactacgccg tctgcaacaa atacggcatc
     5641 gagctttaca atcctgtcaa cgccgaaggc aaatacatcg gcgaaacgcc gcgcgttgcc
     5701 ggtatgcgcg tttgggaggc gaaccccgtc atcctgcaat ggttggaaga aaccggcaac
     5761 cttttggcaa gcagtaaaat cgaacacagc tacgcccact gctggcggca caaaacgccg
     5821 ctgatttacc gcgcgaccgg tcaatggttt gtcggtatgg acaaagccgg tgccgacggc
     5881 aaaaccctgc gcgacaaagc catcaaggcc gtggacgaca ccgaattctt cccgtcttgg
     5941 ggtcgcgcgc gtttggaagc catgattgaa ggtcgtcctg actgggtggt ttcacgccaa
     6001 cgctattggg gcacgccgat gactttcttt gttcacaaag aaacgggcga gctgcatccg
     6061 aactctgccg aacttttgga aaaagttgcc ctgaaaatcg aagaaaaagg catcgaggct
     6121 tggttctccc tcgataaaag cgaattatta agcgcggaag attgcgaaca ttacgacaaa
     6181 ctccccgaca cgatggacgt atggttcgac tccggttcga cccattattc cgttgtgaaa
     6241 cagcgcgaag aattggactg gcctgctgac ttgtacctcg aaggcagcga tcaacaccgg
     6301 ggctggtttc agtcgtccat gctgaccggc tgcgcctcat caatgggtcg cgcgccgtac
     6361 aaacagctgc tgacccatgg tttcgtggtg gatcaaaacg ggcgcaaaat gtcgaaatcc
     6421 atcggcaacg tcgtcgcgcc gcaagaagtg tataacgagt tcggcgcgga catcctgcgc
     6481 ctgtgggcgg catccaccga ttacagcggc gaattggcga tttccaaaga aatcctcaag
     6541 cgcgtaaccg aaagctaccg ccgtatccgc aataccttga gctttttgtt tgccaacttg
     6601 agcgacttca atcctattga agatgccgtg caacaggcgg atatggtgga aatcgaccgc
     6661 tactccgtgg tattggcacg tcagctgcaa gagcgtctgg caggcgatta ctatccgcgt
     6721 tatgccttcc actttgccgt aaaagacatt gtttctttct gctcggaaga cttgggtgcg
     6781 ttctacctcg acatcctgaa agaccgcctc tacaccacca aagccgacag ccatgcacgc
     6841 cgcagcgcac aaactgccct gtatcacatc acacgcagtt tggttctctt gattgcaccg
     6901 attttgtgct tcaccggcga agaagcgtgg gacatcatcg gcggcggcga agaagacagc
     6961 gtcctcttcc atacttggca cgagttcccg accatcaacg aaaaaaccga agccgaactg
     7021 gtgaaaaaat ggacggcaat ccgcgaagcc cgcgaagcgg taaccgccgc catcgagcct
     7081 ttgcgcgccg acaaaaccgt cggttcgtcc ttgcaagccg aagccgaaat caccgcgccg
     7141 gaagaaatgg ccggctacct gaacgccttg ggcgaagaat tgcgctttgc catgctggtg
     7201 tctaaagcag aagtgaaagt aggcagcgaa cttgccgttg ccgctaaagc cagtgatggt
     7261 gaaaaatgcg aacgctgctg gcactacacc cgcgatgtgg gcgcggttgc aggctatgaa
     7321 accgtctgca aacgctgtgc agagaatgtc ggcggagaag gcgaaacgcg ccattacgcc
     7381 tgataaagtt tgagcaaatg ccgtctgaaa ccgccgaaca gcatttcaga cggcattttt
     7441 tgtgccgcga tttgtcttta taacggcgga ggggtttcaa gattgcggtg ttgtcggaat
     7501 gcaactgaac cgtcattccc acgaaagtgg gaatctagaa tctcgaagtt tcagtcattt
     7561 ccgatagatt cccgccgcgt cgtgggtctg gattcccgcc tgtgcgggaa tgacggattt
     7621 cgagattg
//
LOCUS       DAWKQB010000101         7618 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.101, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000101 DAWKQB010000000
VERSION     DAWKQB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.101"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(65..481)
                     /gene="dksA"
                     /locus_tag="WJL14_001550"
     CDS             complement(65..481)
                     /gene="dksA"
                     /locus_tag="WJL14_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ7656601.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     VTTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(625..1416)
                     /locus_tag="WJL14_001551"
     CDS             complement(625..1416)
                     /locus_tag="WJL14_001551"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ7656602.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYIGG
                     TRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTIWLEDEAQMHGI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAINEGVCACVRRSQEMERQYQ"
     gene            complement(1465..1872)
                     /locus_tag="WJL14_001552"
     CDS             complement(1465..1872)
                     /locus_tag="WJL14_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ7656603.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1872..2558)
                     /locus_tag="WJL14_001553"
     CDS             complement(1872..2558)
                     /locus_tag="WJL14_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ7656604.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGRKPHSVSLIAVSKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNAAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2678..3721
                     /locus_tag="WJL14_001554"
     CDS             2678..3721
                     /locus_tag="WJL14_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ7656605.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3884..5110
                     /locus_tag="WJL14_001555"
     CDS             3884..5110
                     /locus_tag="WJL14_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ7656606.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLQRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRINDLNLVS"
     gene            5228..7378
                     /gene="yccS"
                     /locus_tag="WJL14_001556"
     CDS             5228..7378
                     /gene="yccS"
                     /locus_tag="WJL14_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ7656607.1"
                     /translation="MKALPIKSLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDNPDIRHLRRLLDNLGS
                     VDQQFRQLRHSDSPAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1870 a         2548 c         1653 g         1547 t
ORIGIN      
        1 ggtgcgcctt tgtgaatatg ccgtctgaaa ctcggggact cagacggcat tttgtttgcc
       61 gccatcagtc ggcaaactgt tttttcatcc tctctcggcg ttcttgggac tcaacagata
      121 aagtggctgt cgggcgtgcc agcagccgct tcagaccgat aggctctccc gtatcggcac
      181 agaatccata atccccttca tcaatattgc ggatggtcgc ctgtatttta ctgagaagtt
      241 ttcgttcccg atcgcgggta cggagttcca atgcgtactc ttcttcctgt gtggcacggt
      301 cggcaggatc gggggctgat tcgtgttctt ggaggtgccc tgtcgtaaca gatgcattct
      361 ctatgagttc atcttgcatt ttaaccagca attcacgaaa aaaagccaaa tggtcgtcat
      421 tcatataatc gtcttccggc ccattccaat tcaaaatatc ttgttctgtc agctttgcca
      481 taatgtatgt cctctcagtt caccgaaaaa aaagcaccac aaagtggctt tattcctttg
      541 atttttataa ggcttattgc ctaaggcgtt tttgaaaacg cgcatcataa cacgttttgg
      601 attggttttt ttattttctc tacattattg atattgccgt tccatttcct gcgaacggcg
      661 cacacaggca caaacgcctt catttatggc ttcggcgaca cgatgccgtc tgaaggcttc
      721 cacggcttcg tgagttgtcc cgccttttga cgttacattt ttttgaagct tctcgaaatc
      781 ttcaccagtc tgctcggcaa gggcaaccgc tcctttaaat gttgccagac tgagcgcgcg
      841 tgcttctgcc atatcaaacc cttgtcggat ggcggcattt tgcaatgcgt ccagcagata
      901 aaacacataa gccggcccgc tgccgctgat gccggtaatg ccgtgcattt gcgcttcatc
      961 ttccaaccaa atggtcaaac cgactgattt catgattcga tcggcaatcc tgcggtctgt
     1021 ttccgatact tccgcttcgg caaacatacc ggatacgccc agcccgattt ttccgggtgt
     1081 attcggcata acccggacaa tgcggcgtgt tcccccaatg taacggctga gcgtatcgac
     1141 cggcaatccg gctgcgacag aaagcaccaa tgcgccgttg gtgcggatat ttttgcacgc
     1201 ggcttccata tcctgcggct tgacggcaag gattaaaaca tcgtcggaat gaagctccgg
     1261 caaggtttca gaagtttcga cccccaactc tttttgcaaa cgttcgcgct tttccgcacc
     1321 ccggttggca atatggatgc ggtaaccgcc ttgtttgacc aatccgcccg caacggcagc
     1381 cgccatattg ccgccgccga gaaaataaac attcattatt tctaccctat tcaaaattac
     1441 cttactaaat tcattcccaa ttttttacca tcggacgcgc tcggacagac cgttgcgccg
     1501 caacccctgc ttttccaagc agtcaaatcc tccctgtttg aacgtctcaa tcaaaggttt
     1561 gccgtcacgc gtacgccaat ccagcccgca ggcttcaaac ttccctattt tcctgtaaaa
     1621 gtaagattgt tgtgcggatt catattcgtt tgccctgacg gaacgattgc cgtctgaaag
     1681 cggtatctcg tagtcctcta aagacggcgg actctcgccg ccaatatccc aaaaatcgat
     1741 ggcagccggt tttctcggct tacaccagcc ggacagggac gaacactcat accaacccgc
     1801 caactggcag cctgcaaccg taaatgcaag caacgcagcc attgccgtat attttattgc
     1861 ccgaatttcc atcagcccct tttcccgaaa atcgcgctgc cgatacggac gtgtgtcgca
     1921 ccgcactcaa tggcggcagg catatcgtcc gacatcccca tagacagcac gtctgcctta
     1981 acgccagccg cattgaggtc ggcaagcagt ttccgcatcg tttgaaattg caccttcaac
     2041 tccgtttcac tgctgttggc tttggcaaca cacatcagtc cgcgtacgac gatattcggc
     2101 agcttcgcca cttccacagc aagcgcgact gcttcttcgg gcgcgacacc gtgcttcacc
     2161 gcctcgcccg caatgttcac ctcgatacac acctgcaaag gcggcattga ggaaggacgt
     2221 tgcccgctca gccggacagc ggttttcaga cggcatacgg tatgcaccca atgcgcacgt
     2281 tcggcgacaa acttggtttt gttggactgc acatcgccga tgacgtgcca cacgatgtca
     2341 gtcaaatccg ccaactcttc cgttttaccg taccactcct gaatatagtt ctcgccgaaa
     2401 tcacgctgtc cggcggcgta aacttcgcgg atgccgtctg aagggaaagt cttactgacg
     2461 gcaatcaggc tgacggaatg cggcttcctt cccgcctgca gaaccaattt tccgatacgg
     2521 tcggacacct cacaataacg ttcttgcaac accgtcatag attatcccct aattaaaaat
     2581 gattaaacag ttgaaacccc tccagtcagg gcggtacaat caaggttgtt agaaccattc
     2641 caaccaatcg aaacattata ctaaacagag ccgcattatg cagattaccg acttactcgc
     2701 cttcggcgct aaaaacaaag catccgacct tcacctgagt tcgggcatat cccctatgat
     2761 tcgggttcac ggcgatatgc ggcgcatcaa ccttcccgaa atgagcgcgg aagaggtcgg
     2821 taatatggta acttcggtga tgaacgacca ccagcggaaa atctaccagc aaaacttgga
     2881 agtcgacttc tcgttcgaac tgcccaacgt cgcccgattc cgcgtcaacg ccttcaacac
     2941 cggccgcggt cccgccgccg tattccgcac cattcccagc accgtcttat cgctggaaga
     3001 attgaaagcc ccgagcattt tccaaaaaat cgcagaatcg ccgcgcggca tggtattggt
     3061 taccggccct accggttcgg gcaaatcgac cacgcttgcc gcgatgatca actacatcaa
     3121 cgaaacccag ccggcacaca tcctgaccat cgaagacccg atcgaattcg tccaccaaag
     3181 caaaaaatcc ctgattaacc agcgcgagct gcaccagcac accctcagct tcgccaacgc
     3241 gctgcgttcc gcattgcgcg aagaccccga cgttatcctt gtcggcgaga tgcgcgaccc
     3301 cgaaaccatc ggcttggcac tgaccgccgc cgaaaccgga cacttggttt tcggcacgct
     3361 gcacacgacc ggcgcagcaa aaaccgtcga ccgtatcgtg gacgtattcc cggcgggaga
     3421 aaaagaaatg gtgcgctcta tgctgtccga atcgctgacc gccgtcatct cccaaaacct
     3481 gctgaaaacg cacgacggca acggccgtgt cgcctcgcac gaaatcctga ttgccaaccc
     3541 cgccgtccgc aacctcatcc gcgaaaacaa aatcacgcag attaactccg tcctgcaaac
     3601 cgggcaggcg agcggtatgc agacaatgga ccaatcgctg caatcgctgg tgcgccaagg
     3661 gctgatcgca ccggaagtcg cacgcagacg cgcgcaaaac agcgaaagta tgagtttctg
     3721 acacacaacc gccttccggc cataccggcg ggaaaacaag gcgcaaacac gcggggcggg
     3781 acgcagcatc ccgcccggct acctttccga acaagaagcg tccgccttcc tgttgaaacc
     3841 tgccgccgca aactgcaagg cttaaaccga aaagaagcta acgatgaata ccgataacct
     3901 gcacgacatc ttggacgaaa tggttcaagt gtattctcaa aaaaaacaaa gccgatccga
     3961 aaccccggcc gaaatcggcg cacacttcca cccgctgctc gaccgcctgt gcgaaaccgc
     4021 agaagcacaa aacgcgtccg acatccttat cagcaaagga ttcccgccct cgttgaaaat
     4081 caacagcgca ttaaccccgc agccgcaaaa ggcgctgacg ggcgaggaaa ccgccgccat
     4141 cgccgcatcg acgatgaacg ccgaacaatc ggaaatattc cggcgcgacg gcgaaatcaa
     4201 ctactccgtc cagtcgcgca gcggcacgcg ctaccgcgcc aacgcctacc acagccaagg
     4261 cagcgcaggt ttggttttgc agcgcatcaa ccacgtcatc ccgcaaatgc aggaattggg
     4321 cctgcccgaa aaactcaaag acctcgccgt cgcaccgcgc gggctgctga ttatcgtcgg
     4381 gcctaccggt tcgggcaaat ccaccacgat ggcgactatg ctcgaacacc gcaacaaaac
     4441 cctgcccagc catatcgtta ccatcgaaga cccgattgag tttatctaca aaccgcgccg
     4501 ctgcatcttt acccagcgcg aaatcggcgt cgacaccata aactggcaga cggcggtaca
     4561 aaacgctatg cgccagtccc ccgacgtggt ctgcatcggc gaagtccgca gcagggaaag
     4621 tatggaatac gcgatgcagc tcgcccaaac cggccacctg tgcattttta cgctccacgc
     4681 caacaccgcg ccgcagtcgc tcgaacgcat actcaacttc taccccaaag aacagcacaa
     4741 ccaaatactg atcgacatcg ccctcaacct gaccggcatc atctgccaac gcctcgccct
     4801 caaacaagac aaaacgggca ggacggcggt tgtcgacttg ctcatcaaca cgcccgccat
     4861 ccaagacttc atcctgaagg gcgacctgat gaacatcagt aaaatcatgg aaaccgccaa
     4921 aaccgacgga atgcagacga tggatcaaaa ccttttcgaa ctgtaccgtc acggcatcat
     4981 cagttacgaa gaagccctgc gccagtccgt ttccgccaac aacctgcgat tgcacatcca
     5041 actgcacaaa gaaggcaaaa cgcccgaact cctttacgac aggattaacg acctcaacct
     5101 cgtttcctaa ttgaagaaac acccgatgcc gtctgaaaac ccctgccgtt ttcagacggc
     5161 atcgcttcat catccgcatc cgtgtgctac acttcattta cctcataaca ataaatccga
     5221 caaacccatg aaagcacttc cgatcaaatc actcctcatc acctcgcttc ccgttttcgc
     5281 cagtgttttt accgccgcct ccatcgtctg gcagctaggc gaacccaagc tcgccatgcc
     5341 cttcgtactc ggcatcatcg ccggcggcct tgtcgatttg gacaaccgcc tgaccggacg
     5401 gctgaaaaac atcatcgcca ccgtcgccct gtttaccctc tcctcgctca cggcacaaag
     5461 taccctcggc acagggctgc ccttcatcct cgccatgacc ctgatgacct tcggctttac
     5521 cattttaggc gcggtcgggc tgaaataccg caccttcgcc ttcggcgcac tcgccgtcgc
     5581 cacctacacc acacttacct acacccccga aacctactgg ctgaccaacc ccttcatgat
     5641 tttatgcggc accgtactgt acagcaccgc catcatcctg ttccaaatca tcctgcccca
     5701 ccgccccgtc caagaaagcg tcgccaacgc ctacgacgca ctcggtggct acctcgaagc
     5761 caaagccgac ttcttcgacc ccgatgaggc agcctggata ggcaaccgcc acatcgacct
     5821 cgccatgagc aacaccggcg tcatcaccgc cttcaaccaa tgccgttccg ccctgtttta
     5881 ccgccttcgc ggcaaacacc gccacccgcg caccgccaaa atgctgcgct actacttcgc
     5941 cgcccaagac atacacgaac gtatcagctc cgcccacgtc gactaccaag agatgtccga
     6001 aaaattcaaa aacaccgaca tcatcttccg catccaccgc ctgctcgaaa tgcagggaca
     6061 agcctgccgc aacaccgccc aagccctgcg cgcaagcaaa gactacgttt acagcaaacg
     6121 cctcggccgc gccatcgaag gctgccgcca gtcgctgcgc ctcctttcag acagcaacga
     6181 caatcccgac atccgccacc tgcgccgcct tctcgacaac ctcggcagcg tcgaccagca
     6241 gttccgccaa ctccgacaca gcgactcccc cgccgaaaac gaccgcatgg gcgacacccg
     6301 catcgccgcc ctcgaaaccg gcagcctcaa aaacacctgg caggcaatcc gtccgcagct
     6361 aaacctcgaa tcaggcgtat tccgccatgc cgtccgcctg tcccttgtcg ttgccgccgc
     6421 ctgcaccatc gtcgaagccc tcaacctcaa cctcggctac tggatactac tgaccgccct
     6481 tttcgtctgc caacccaact acaccgccac caaaagccgc gtccgccagc gcatcgccgg
     6541 taccgtactc ggcgtaatcg tcggctcgct cgtcccctac ttcaccccgt ctgtcgaaac
     6601 caaactctgg attgtcatcg ccagtaccac cctctttttc atgacccgca cctacaaata
     6661 cagtttctcc accttcttca ttaccattca agccctgacc agcctctccc tcgcaggttt
     6721 ggacgtatac gccgccatgc ccgtacgcat catcgacacc attatcggcg catcccttgc
     6781 ctgggcggca gtcagctacc tgtggccaga ctggaaatac ctcacgctcg aacgcaccgc
     6841 cgcccttgcc gtatgcagca acggcgccta tctcgaaaaa atcaccgaac gcctcaaaag
     6901 cggcgaaacc ggcgacgacg tcgaataccg cgccacccgc cgccgcgccc acgaacacac
     6961 cgccgccctc agcagcaccc tttccgacat gagcagcgaa cccgcaaaat tcgccgacag
     7021 cctgcaaccc ggctttaccc tgctcaaaac cggctacgcc ctgaccggct acatctccgc
     7081 cctcggcgca taccgcagcg aaatgcacga agaatgcagc cccgacttta ccgcacagtt
     7141 ccacctcgcc gccgaacaca ccgcccacat cttccaacac ctgcccgaaa ccgaacccga
     7201 cgactttcag acagcactgg atacactgcg cggcgaactc gacaccctcc gcacccacag
     7261 cagcggaaca caaagccaca tcctcctcca acagctccaa ctcatcgccc ggcagctcga
     7321 accctactac cgcgcctacc gacaaattcc gcacaggcag ccccaaaacg cagcctgaaa
     7381 aagtttcggc attttgtaag agagggcaga ttgtcagaca ggttgcaaga tagtggataa
     7441 agctttgcgc agggtaaatg cgtagcaact gaaccgtcat tcccacgaat ctacattccg
     7501 tcattcccac gaaaacagaa aaccaaaaac agaaacctaa aatcccgtca ttcccacgaa
     7561 agtaggaatc cagttttttg agtttcagtc attcccgata aattgcctta gaattgaa
//
LOCUS       DAWKQB010000102         7556 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.102, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000102 DAWKQB010000000
VERSION     DAWKQB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.102"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            15..419
                     /locus_tag="WJL14_001557"
     CDS             15..419
                     /locus_tag="WJL14_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ7656608.1"
                     /translation="MGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAG
                     HGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGW
                     LLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL14_001558"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WJL14_001558"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ7656609.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRVAFPVVMFETPHRIGATLADMAELFPERRLMLAREITKTFETFLSGTVGEIQT
                     ALAADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            1382..1729
                     /locus_tag="WJL14_001559"
     CDS             1382..1729
                     /locus_tag="WJL14_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ7656610.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1734..2327
                     /locus_tag="WJL14_001560"
     CDS             1734..2327
                     /locus_tag="WJL14_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ7656611.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            2391..2999
                     /locus_tag="WJL14_001561"
     CDS             2391..2999
                     /locus_tag="WJL14_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ7656612.1"
                     /translation="MKPKPHTVRTLTAAVLSLALGGCVSAVVGGAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3007..3657
                     /locus_tag="WJL14_001562"
     CDS             3007..3657
                     /locus_tag="WJL14_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656613.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHASS
                     SAPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGAQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3697..4476
                     /gene="map"
                     /locus_tag="WJL14_001563"
     CDS             3697..4476
                     /gene="map"
                     /locus_tag="WJL14_001563"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ7656614.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4631..4930
                     /locus_tag="WJL14_001564"
     CDS             4631..4930
                     /locus_tag="WJL14_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ7656615.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5168..5542
                     /locus_tag="WJL14_001565"
     CDS             5168..5542
                     /locus_tag="WJL14_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ7656616.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5730..7196)
                     /locus_tag="WJL14_001566"
     CDS             complement(5730..7196)
                     /locus_tag="WJL14_001566"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ7656617.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERT
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1943 a         2284 c         1851 g         1478 t
ORIGIN      
        1 aagaggcgga atgggtgggc agcctgttcg gcctgcacaa atctttcggt ttccttacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cggttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaagtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 atcgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcgccgcgcg attggttgcc
      661 gtccgccgcc aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtgatt tcgcgcgcca gcattaatcg gcgttcgggg aacagttccg ccatatcggc
      781 aagcgtcgcc ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccacccgcac
      841 ccatttggca aacaatttcc tacgttcgcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cagccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga cgagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaaatgttt
     1321 ctgaaacata agaatgccgt ctgaaaaaca aacattataa attataaagg ttaaaccgat
     1381 tatgcgccta aaccacaaac agggcgaggc aggggaagat gccgcgcttg ccttcctcca
     1441 atcccaaggc tgcacactgc ttgcccgcaa ctggcactgc gcctacggcg aaatcgacct
     1501 gattgtcaaa aacggcggca tgattctgtt tgttgaagta aaataccgca aaaatcggca
     1561 attcggcggt gccgcataca gcatttcccc atccaaatta ttgaaactgc aacgaagtgt
     1621 agagtattat ctgcaacaga acaggttgac aaacgtaccg tgccgcctcg acgcggtact
     1681 catcgaaggc agccgcccgc ccgagtggat acagaatatt acaggttgac gatatgacga
     1741 cattacaaga acgcgttgcc gcccattttg ccgaaagcat ccgtgccaag caggaagccg
     1801 gaaaagtatt ggtcgagccg accgtacagg ctgccgagct gatgctgcaa tgcctgatga
     1861 atgacggcaa aatcctggcc tgcggcaacg gcggttcggc tgccgacgcg caacacttcg
     1921 ccgccgaaat gaccggccgt tttgaaaaag aacgcatgga actcgccgct gtcgcgctga
     1981 caacagacac ttccgcgctg acagccatcg gcaacgacta cggtttcgac cacgtattca
     2041 gcaaacaggt gcgcgcgctc ggacgtgcag gcgatgtatt ggtcggcatt tccacctccg
     2101 gcaattccgc caacgtcatc gaagccgtca aagccgcaca cgaacgcgat atgcacgtca
     2161 tcgccttgac cggccgcgac ggcggcaaaa tcgccgccat actcaaagac accgacgttt
     2221 tgctcaacgt tccccatccg cgcaccgccc gtattcaaga aaaccacatc ctgctgatac
     2281 acgccatgtg cgactgtatc gactccgtac tgctggaagg aatgtaaccc ttttcagacg
     2341 gcatggcgca aagcaatgcc gtctgaaacg cccaagaaag gaagcacccg atgaaaccca
     2401 aaccgcacac cgtccgcacc ctgactgccg ccgtcctcag ccttgccctc ggcggctgcg
     2461 tcagcgcagt cgtcggcggc gcggcggtcg gcgcgaaatc cgccgtcgac cgccgaacca
     2521 ccggcgcgca aaccgacgac aacgtaatgg cgctgcgtat cgaaaccacc gcccgctcct
     2581 atctgcgcca aaacaaccaa accaaaggct acacgcccca aatctccgtt gtcggctaca
     2641 accgccacct gctgctgctc ggacaagtcg ccaccgaagg cgagaaacag ttcgtcggtc
     2701 agattgcacg ttccgaacag gccgccgaag gcgtgtacaa ctacattacc gtcgcctccc
     2761 tgccgcgcac tgccggcgac atcgccggcg acacttggaa cacatccaaa gtccgcgcca
     2821 cgctgttggg catcagcccc gccacacagg cgcgcgtcaa aatcgttacc tacggcaacg
     2881 taacctacgt tatgggcatc ctcacccccg aagaacaggc gcagattacc caaaaagtca
     2941 gcaccaccgt cggcgtacaa aaagtcatca ccctctacca aaactacgtc caacgctgac
     3001 tcggcaatgc cgtctgaacc gccttcagac ggcattgccc gacaccccaa aagcacaatc
     3061 aaaatggcaa aaaaaccgaa caaacccttc aggctgaccc ccaaactcct gatacgcgcc
     3121 gtattgctca tctgtatcac cgccatcggc gcattggcaa taggcatcgt cagcacattc
     3181 aacccgaacg gcgacaaaac cctccaaacc gaaccgcaac acaccgacag cccccgcgaa
     3241 accgaattct ggctgccaaa cggcgtagtc ggacaagatg ccgcccaacc cgaacaccac
     3301 cacgcctcct catccgcacc cgcacagccg gacggcacag acgaaagcgg cagcggactg
     3361 ccgtcccctg ccgcacccaa gaaaaaccgg gtcaaaccgc aacctgccga cacagctcaa
     3421 accgacaggc agccggacga cgccggagca caagctgaaa acacactcaa agaaaccccc
     3481 gtactgccca caaacgtccc ccgtcccgaa ccccgaaaag aaacacccga aaaacaggca
     3541 cagcccaaag aaacacccaa agaaaaagaa acgcccaaag aaaaccatac caaaccggac
     3601 accccgaaaa acacgccgcc taaaccccat aaagaaattc tcgacaacct cttctgaccc
     3661 ggcacggcag gcacacccgc aatccaagga agcattatga acggcatcat catcaaaacc
     3721 cccgaagaaa tcgaaaaaat gcgcgagctg ggcaaactcg tcgccgaagc cctcgactac
     3781 atcggacaat tcgtcaaacc cggcgtaacc accgacgaaa tcgacaaact cgtttacgac
     3841 taccacgtca acgtccaagg cggctatccc gcccccctgc actacggcaa cccgccctac
     3901 cccaaatcct gctgcacctc cgtcaaccac gtcatctgcc acggcattcc cgacgacaag
     3961 ccgctcaaag aaggcgacat tatcaacatc gacctcacca tcaaaaaaga cggcttccac
     4021 ggcgactcca gccgtatgtt taccgtcggc aaagtctccc ccatcgccca acgcctgatc
     4081 gacgtaaccc acgcctccat gatggcgggc atagaagccg tcaaacccgg cgcgacactg
     4141 ggcgacgtag gttacgcctg ccaacaggtt gccgaaaacg ccggctattc cgtcgtacag
     4201 gaattctgcg gacacggcat cgggcgcggt ttccacgaag ccccgcaagt gttgcactac
     4261 ggaaaaaaag gacagggctc cgttctaaaa ccgggtatga tttttaccgt cgaaccgatg
     4321 atcaaccaag gcaaacgcca cctgcgtatc ctcaacgacg gctggacggt ggttaccaaa
     4381 gaccgctccc tctccgccca atgggaacac gaagtcttgg tgaccgaaac cggctacgaa
     4441 atcctcaccg tcagccccgc ctccggcaaa ccctgaagcc ggacgtatcc gccccataaa
     4501 aacaaacaat gccgtctgaa agaaacggca gatatgatat ataatataaa aacaggcttg
     4561 acccggcaca ttacgaaaac aaagcaaatc ggaatttgcc ccgcaaccag acaaacttaa
     4621 aggaagtttt atgaaaatat ttgaaaatat agaagatgtt aaagccatcc gtaaaaagac
     4681 cgggctgaac cagatagact tctggggcaa ggtcggcgtt acccagtctg gaggctcgcg
     4741 ctacgaaacc ggccgcaaaa tgcccaaacc cgtacgcgaa ctgctccgcc tcgtccatat
     4801 cgaatgcatc gatttggcga aagtcaacaa aaaagatatg gaaatcgccg ccctgttgaa
     4861 aaaacaccat cccgacctgt atgccgagtt gtccaaacag accaagtccg aaagaaaaaa
     4921 acaaagttaa accgcaacct ccggatgccc gaaggttttt catttccgaa aaacgcaaac
     4981 aatgccgtct gaaacaccgg acaggccgcc gtatcccgcc tgccgcccct gcctcaaacc
     5041 gccgaaccgc ccgaacccgc ctttttacaa actttatcca atttcctgtt tatttcagga
     5101 tacgccgaca ttagaatgtc aaaccgtccg aaacgggcaa actccacatc catccaaagg
     5161 aataaaaatg aaacttctga ccaccgcaat cctgtcttcc gcaatcgcgc tcagcagtat
     5221 ggctgctgct gccggcacga acaaccccac cgttgccaaa aaaaccgtca gctacgtctg
     5281 ccagcaaggt aaaaaagtca aagtaaccta cggctttaac aaacagggcc tgaccacata
     5341 cgcttccgcc gtcatcaacg gcaaacgtgt gcaaatgcct gtcaatttgg acaaatccga
     5401 caatgtggaa acattctacg gcaaagaagg cggttatgtt ttgggtaccg gcgtgatgga
     5461 tggcaaatcc tatcgcaaac agcctattat gattaccgca cctgacaacc aaatcgtctt
     5521 caaagactgt tccccacgtt aatcaggcaa caaaaaacag cgttttcaga aatgaaaacg
     5581 ctgttttttt gaccgttcca ttattcgcaa aagggaaaaa acgattacct accccgtgta
     5641 ccaaaacctg ccctgccgga tgaagggcat aaccggcagg gacggcatca acaccatatg
     5701 ggggtacggc ttttcttgga agattcggtt taaatatcca acactttcgc ggtataggcg
     5761 ataatttcat ccgccctttc ggggttttcg ttcaacttga tgccgtaacc cggtaccagc
     5821 tctttcagac ggccttccca agacggggtg cgttcgggga agcattggtg catcagccgg
     5881 atcatcagcg gcacggcggt cgatgcgccc ggcgacgcgc ccagcaatgc ggcgagcgag
     5941 ccgtcggcgt gtgcgacaat ctccgtacca aactgcaaca cgccgccttt ttcggagtct
     6001 tttttaatga tttgaacgcg ttgccctgcg gtgatgagtt cccagtcgtc ggggtttgcc
     6061 tcggggtagt attccagcag ggaggcgaag cgttcttctt tggttttacg caattcgccc
     6121 agcaggtatt tggtcagcgg catattcgcc cagccggcgc gcagcatagg atagaggttg
     6181 tccatatgga tggacagcgg caaatccata agtgagcctt gcttgaggaa gttggaacgg
     6241 aagcctgcgt aagggccgaa cataaggtgg cgtttgccgt ccacgttgcg tgtgtcgagg
     6301 tgcgggacgg acatcggcgg cgcgccgacg gaagcctgcc cgtacacttt ggcgttgtgt
     6361 tgttcggcgg tttcggggtt gctgttgcgg aagaacaggc cggacacggg aaagccaccg
     6421 tagcctttgc cttcggggat gccggatttt tgcagcaggg tcagcgcgcc gccgcccgcg
     6481 ccgaggaaga ggaagcgggt acggagggtg agctgcccgt cggggttgcg ggtatcggcg
     6541 gttttgagca cccacgcgcc gtcggattcg cgtttgatgt cttcgacgtg gcggttgaac
     6601 tcggttttta cgcccttgcc ctgcaaatat ttcaccattt ggcgcgtcag ccgtccgaaa
     6661 tcgacatccg tgccttcggc ggagtagttg gcggcgacgg gttggttttc gtcccggccg
     6721 cgcatcatca gcggagccca atcggaaatt ttgttccgat cggtggaaaa ttccatattt
     6781 tcaaaaagtt tttgggtttt aaacgcgtca taacgttttt gaagataaga acaatggtct
     6841 tcattcatca ccaaagacat atgcggcacg gcattgatga aggaattgtc ttccaacttg
     6901 ccttccgcga ccaacgtcgc ccaaaactgg cggctgacat gaaactgttc ggcaatattg
     6961 agggcgcgcg ccggatcgat aatcccattt gcacccaacg gcgcatagtt caattcgcac
     7021 agcgcggaat gccccgtgcc ggcgttgttc cacgcgtttg acgattccaa cgccacatct
     7081 tccaagcgtt caatcagggt gatttcccaa gacggttcga gttctttgag caaaacgccc
     7141 aaagtcgcgc tcataatgcc gccgcccacc aagacaacgt ctgtcgcttc agccatggtt
     7201 tactcctaaa aaacaggcat cttctgccct tatggttatt tgccgtacta caaacgcctg
     7261 aatcgcaaaa gcagggaaaa ccggcaatgg tgtgtgtccg agtatgctgt ttcagggttg
     7321 gaatgcgttg caagtatggc ttccgacacc gcttcagggg cttgtaatat gttatcgtga
     7381 atgtagtgga ttttactggg aaatgcaaag tttttctgtc gcccgccaag cccgggaact
     7441 gcgaaatgaa aaataaaaat agttatttat ctatatatat caaattttta atagataaaa
     7501 aaccaaaatt gtttatatat taatttttaa aagattgtca gcatattgcg ttaagt
//
LOCUS       DAWKQB010000103         7334 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.103, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000103 DAWKQB010000000
VERSION     DAWKQB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.103"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            200..412
                     /locus_tag="WJL14_001567"
     CDS             200..412
                     /locus_tag="WJL14_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ7656618.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILESVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            560..2143
                     /locus_tag="WJL14_001568"
     CDS             560..2143
                     /locus_tag="WJL14_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ7656619.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLSAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLDRRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSVD"
     gene            2225..3322
                     /locus_tag="WJL14_001569"
     CDS             2225..3322
                     /locus_tag="WJL14_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ7656620.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGHNPFFSA"
     gene            3436..4551
                     /locus_tag="WJL14_001570"
     CDS             3436..4551
                     /locus_tag="WJL14_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ7656621.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQAIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4676..5131
                     /locus_tag="WJL14_001571"
     CDS             4676..5131
                     /locus_tag="WJL14_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ7656622.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5202..5678
                     /locus_tag="WJL14_001572"
     CDS             5202..5678
                     /locus_tag="WJL14_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ7656623.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5666..6592
                     /locus_tag="WJL14_001573"
     CDS             5666..6592
                     /locus_tag="WJL14_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ7656624.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6657..7214
                     /locus_tag="WJL14_001574"
     CDS             6657..7214
                     /locus_tag="WJL14_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ7656625.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATHGLYQNVPVQPVKIRRIVVGQ"
BASE COUNT         1917 a         1802 c         1914 g         1701 t
ORIGIN      
        1 tgcgccgatt gtcaaggcga tgatgctcaa ccagggtttt tgcgaagcgt tcattgttgt
       61 cgtcctgatg ggaattgccg ccattatgcg caagcgtccg tctttttcca aaccgccgca
      121 ccgtttccgc gtttgcccaa gttcacgccc cgacttacaa taacgcccgt cagattgcag
      181 ttccaacgaa aggaaaatga tggaaaccct tatcctcgac atcggcggca tgagctgcgg
      241 cggctgcgtc aaaagcgtta cccggatact ggaaagcgtc aaaggcgtgg caagcgtcga
      301 agtcagcctt gaaaacaaaa gtgcgaccgt cggttacgat cccgcgcaaa ccgatgcagg
      361 ggcgttgatt gaagccgttg aagacggcgg ctatgatgcg gcgttgaaat aaagcggcaa
      421 aaatgccgtc tgaagccttc gcgcctccca gatgccgtct gaagtacgct ccgcgccttc
      481 agatggcatt tgatttagtt gaacgggtag gatgaattgg ttaaacttgc gggcaatagt
      541 gccttcagac aaacaaaata tgccgaacaa aaagaaaaaa gccgcctttg cgcgtgctgc
      601 aacggagaac ggtgtgccgg acgtgtccaa catcaaaaat tcatatgatg atttgatgta
      661 tgaatccaaa gcattttccc aaaccgccat caacgccttg gaggctcgcg cccgcatgat
      721 gggtttgcag cccgcgcccg ccgccaatgc caaggtcttg gagctgggct gttcgatggg
      781 cggcaacatc atcacgcagg ctctttatta cccggatgcg gaatttgtcg gtatcgactt
      841 gtccggcagg caggttgcgc agggcaacgc catcattgaa aaaatgggct tgaaaaatgt
      901 gcgtctggaa gaaaaagata ttttgaccgt cgatgagtca ttcgggaagt ttgactatat
      961 catcgtccac ggcatttggt cgtgggtgcc tgacgcagtt aaagacaaaa ttttttcgat
     1021 ttgccggaac aacttgaccg aacacggcat tgcctatatt tcatacaatg tttaccccgg
     1081 ctggaaacgg caggagcagt tgcgcgaaat tatgtacttt gccggcaggg atgtgcttga
     1141 agaacccttg gaagcacgga cgcggaaagg cttggacgcg ctcaaggcgc tggcggaaat
     1201 tttagaaaac gacaagggct tggacggcgg cggcaaactt tcggcgattc aaaaaatatt
     1261 gaatcataat ttttattaca tcgcacacga atatatggag gcgtttaacg acccgattta
     1321 cgtcaacggc ttcatcgaat gggcaaaccg ccaccggctg gcgtatatcg gggataccga
     1381 tttgcacgcg tcttttgttt cttggatggc ggagcatacg cgggagcgga ttctggcatt
     1441 ggcagggggc gattatattg ccaaagaatt ttacagcgat attttatccg accgccaatt
     1501 ccgccgttcg cttttatgcc gtgaggaagt cggggatact gtcaggcgtg acgagtcggt
     1561 tgccgtcgaa gtgatagaaa gtttgaattt ccgcccggca agaggggaaa caatcaactt
     1621 tgatgaaaac gataccctgc tttcgggcat acgcgatgtg atgaaaaccg gagaggcgtt
     1681 taaaacggaa gatgttgcgg aaaatctcga ccgccgattt cccggtctgg aatttgaccg
     1741 catgaaaatc aattcccagc ttttattgca aaccattctc gggcgttttt ctgtttcatc
     1801 agacaatgcg ggcaaaccat tttttgaaga ccataaaacc tatgtgccgg cgcgttttac
     1861 agattatgcc gccgcctttg tggaacacgg cgcggaagcg tttgttcggc ctgccaaccg
     1921 ttacaacgaa agcaccccct cattcggata cgggcatttg tacattatgc ggcaattatc
     1981 gcggccgacg agcaaacagg cattgattga aacggttgcc gaaaacttga acatcgtcag
     2041 caccacgccc gacggtttga cattccatcc gcctgccgaa gtgtatgtgg aagaaatatt
     2101 ggcagacttg gcagacaggc attttctcgt ttcggtggat tgacgggcaa gtcccggcgg
     2161 caatccgtcc gatatatttt cagacaggaa agacacacac tgcgaaaagc ggtacacgac
     2221 acatatgaac cagacattta ccttgcccga tacgcgcccg tatccccaaa atccgattaa
     2281 gaaccacctg ctgctcaatg cctaccagtt ggcgcacaat tcttcccagg cttcgcgcaa
     2341 actctcgtcc ggccaacttc aaaccgaaat cagggggatg cttgagcaaa accactatat
     2401 caacctttcc ctcgcgctga cgatgtcgcc cgatgccgga acttatgccg cgctgctttc
     2461 cagtgtgaac gcggtactcg attgcgagaa agaaggcgaa gtgcagtggt tcgccctgcc
     2521 ggtcgtgctg gtgtccggct gcaagaaaga acgggcaatc gagatgaagc tgccgacgga
     2581 gggattgttt gcctgtttgc aaaactatcc gcacctgcgc gcgttgacgc aagagacgca
     2641 atggctgcct tatcttgtac attcctccga tttgagtgcg gtcgcgccgg atgagtggtg
     2701 gcgtgccaaa caaaataccg aagcggcggc gcaacactta cgccgtttcg ccccgcgccc
     2761 tttgcttttg cccgaagggc agtccgtcca cgtcgtttac gcgctgggtt tcggcagcgg
     2821 caaagttcag acggcattgg gtcagaacct tcttcaggca ggtctgccct tgatgcaggt
     2881 atggcaggaa aatcttgcat cggaaggcgt tacgctgttt gccaatccgc tttccccgga
     2941 ttctccggta cgcgcgcttt ccgacggcag ccacacgcgc cagcgcatgg cgatggatgt
     3001 gtttgcggca aacgcgatac gcgccgtccg tatgcagagt ccgcgcgtcg gcgtggtcgc
     3061 tgcggcaaag gcgggcggac aggttttatt cgggtttaat gcgaccgacg gcgcgtttga
     3121 agtcgtgccg caggtgtttt cgtggcagct ttcctttacc gacaatattg gcgttattca
     3181 gcaaaatttt ttggatttaa tggcagaatg ccgtgtagaa cacgtttacc tgttgcacaa
     3241 tcccttgggc gaacaggaaa acatcccgag ctatgcggaa gcattgaaac gggaagggca
     3301 caatccgttt ttcagcgcat agtgatttgt agtagtgata ggctttctca tttctataat
     3361 aaatcaaaat aaagagcaca acactttttc attctgtgtt gtgccttgag tgaaacgaaa
     3421 ggatgaatta taaatatgaa gattgtaatt gcaccggatt cgtttaagga aagcttgaca
     3481 gctcaacagg tagctgaagc aataaaaaga ggcttccaac aagcgatagc agatgtggaa
     3541 tgtctcctct gtcctgtcgg tgatggggga gaaggcactg tagatgctat ccgacattct
     3601 cttgacctag aagaaaaatg tctccaagtg acaggacctt ttggacaaaa agaagtcatg
     3661 cgctattttc aaaaagaaca actagcccta ttcgaagttg ctgatttggt tggtcttgga
     3721 aaaatcccgc tagagaaacg aaatccatta caaatccaaa ctcgtggtat tggagaattg
     3781 attcgccacc tcattagtca agaaattaaa gaaatctata ttggcgttgg cggtacggcc
     3841 agtaatgatg gaggtattgg gattgctgct ggtttaggtt atcaatttta tgatgaggat
     3901 ggaaatgcct tacccgcttg cggtcaatcc ttactaaacc tagcttctgt ttcaacagaa
     3961 aatcgctata aaattcctga agatgttcac attcgtattt tagcagatgt tgtgagtccc
     4021 ttatgtggac accaaggtgc gacctatacg tttggcaagc aaaaagggtt ggattctact
     4081 atgtttgagg ccgtagatca ggcaatacaa gatttttatg aaaaagtctc ccctgcaaca
     4141 ttaaaactta aaggagcagg agctggtgga ggcatcgctg gtggtttgtg cgcctttgct
     4201 caggcaagta tcgtatctgg aattgacacc tgcctggact tgattgactt tgataagaaa
     4261 gtgtcagatg ttgacttggt tatcattggt gaaggaagac tagatcgtca aagtttagca
     4321 gggaaagcgc ctattggtgt agcaaaaaga acccctgtcg gagttcctgt tgtcgctatt
     4381 tgtggcagtc tcgttgaaga tttgccttcc ctgccatttg aaaatatcca agctgccttt
     4441 tctattttgg agaaaagtga acctttagaa gatagtttga aaaatgccag tctctatttg
     4501 gagcatacgg cttctaatat tggacactta ttaaatatgc ctaagattta gccaaaccat
     4561 ttcttccaga tggatgtttt ggctggttct gccttatgcg gttacgatta accgaccgat
     4621 gccgtctaaa agggtttccg cttcagacgg catcgcgccg gaacagggag gggatatgaa
     4681 aaaaccgaaa atcctttttg tctgcctcgg caacatctgc cgttcgccga tggcggaata
     4741 cattttgcgc cgccgcgccg ccgaagcggg cattcccctt gaagcggaca gcgcggggac
     4801 atcgggctgg cacgacggcg aagatatgca ccgcgagacg gcaaagatat tgaaaaaaca
     4861 cggtatcgat gcttcaggct ttaccagccg caaaatccgc caaagcgatg cggcggcgtt
     4921 tgactgcatc atcgcgatgg acggcaagaa tttgtccgaa ttggaaagaa ccttcggcag
     4981 gcggccggaa aaaatattca agctgaccga cctgataccc gaaagcggtt acgaccatgt
     5041 ccccgatccg tggtatacgg gcgattttga agaaaccttc aggcttgcgg atgcgggctg
     5101 tcgggcattg ttggaaaaga tttccaaata aagcaattga atacaaatat aaaaccgcct
     5161 cctgccgtat cggttgttca gacggcataa acagagaatt tatgaaaaca aatttcaaac
     5221 agaaaattat cgaacaggca cgcagcgaag gcttgcaggt aaccgctttg cgcgagcagg
     5281 tattagatat tgtcttgcag caaagcggcg tgattaaagc ctacaacgtc ttgtcgcaga
     5341 tgcagcagca aagcgagggc gtgcttgcgc cgccgaccgc ctaccgcgcc cttgattttt
     5401 gggcggagca gggcgttttg cacaaagtgg cggcggtcaa cggctatatt ttgtgcagcc
     5461 acgcgcagca cgagtgcgac gaccattgcc acgaccacga agaagccgaa gcgcaccaca
     5521 gcgcgtttat tttggtctgc accgaatgcg gcacggcgga cgagcaaacc ctcagccacg
     5581 agtgggcggc actgcgcgca ggcgttgccg aaagcggctt tgcgctgaaa gaagaacacg
     5641 ttgttttaac cgggatttgt aaaaaatgtc agaaatgaaa aaaaccaaag tccacctgat
     5701 ttcaggtttt ctgggaacag gcaaaaccac cgcgctcaaa agcctgatgg cgcagaaaga
     5761 cccgaacgaa aaatgggtca tcatcgtcaa cgagttcggc gaaatcggca ttgacggcgc
     5821 ggtattgagc gacaacggca tccctgtggc agaaatcgcc ggcggctgtt tgtgttgcac
     5881 cgccggcccg caaatgggcg taaccgtgca gaaaatgctg cgcgacgcca agcccgaccg
     5941 cctgatgatt gaagcaagcg gactggctca cgcagccagc gtcatcgacg aactcaaagc
     6001 caaaccgctg gacagccttt tggaaatcgg cgccgtcttt accgtcgtca atccgcgcca
     6061 gttcatcaac cccgattacg cgcaacaggc gttgtataaa gaccaaatcg gcatctgcga
     6121 cgtattggtc gccagcaaaa ccgatttatg cacccccgaa cagcttgccg aatttcacga
     6181 caaagcggca aaactgttcc cgcccaaagc caaagtggtt gaagtccaaa acgcacaact
     6241 cgacatccgg tggctcgata ttcccgtcat cgaaaaatca cgctaccgcc tcaaagccct
     6301 gccggataat accatgggct tccagtcgca aggtttcaca ttccccgccg gacgcgattt
     6361 cgacggcgaa aagctgacca actttttcaa cgacctgccc aaaatgaccg aaggactcgt
     6421 ccgcgccaaa ggcgtgtttc aagtattggg aacgtgggtg tggctcaact gggtagacgg
     6481 acagtggggc gcgaaccaag tctcatggcg gcgcgattcg cgttttgaat tgattgccaa
     6541 atcgtttgat gcggatttaa tcgaacaaaa gctcaaagac gcattggaat agcgttcacg
     6601 cgccttcaga cggcatatga aatgaaaatg ccgtctgaag gtctgaggag gacgggatga
     6661 aacccaaatt caaaaccgtt ttaaccgcgc tgcttttggc ggtttccctg ccgtctatgg
     6721 cggcaaccca tgttttgatg gaaaccgata tgggcaatat ccgtttggtt ttggacgagt
     6781 ccaaagcccc caaaaccgtt gctaatttcg tgcgctatgc ccgaaaaggc ttttacgacg
     6841 acaccgtttt tcaccgcgtt atcgacggtt tcgttatcca gggcggcgga tttaccgaag
     6901 acttggtaca aaaggcaacc gataaggccg ttgccaacga atccggtaac ggcttgaaaa
     6961 acactgccgg taccatcgcc atggcgcgga cggccgatcc ggattccgcc accagccaat
     7021 tctttatcaa tctggcggac aatgattcac tcaactacaa aaacggacaa tacggctata
     7081 ccgttttcgg cagggtcgaa agcggcatga acaccgtttc caaaatcgcc cgcgtcaaaa
     7141 ccgccacgca cggcctttat caaaacgtac ccgtacagcc cgtcaaaatc cgtcgcattg
     7201 ttgtcggaca gtaacacgca gacagatgtt cagacggcgt cgcccgtttc acaaaaaacg
     7261 ccgtctgaag tgaaaaaata ttttaaaaca gatagttgat attgacaaat tcaaaccgag
     7321 gattttaaaa tgct
//
LOCUS       DAWKQB010000104         7310 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.104, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000104 DAWKQB010000000
VERSION     DAWKQB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.104"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(195..1064)
                     /locus_tag="WJL14_001575"
     CDS             complement(195..1064)
                     /locus_tag="WJL14_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ7656626.1"
                     /translation="MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
     gene            complement(1159..2076)
                     /gene="hisB"
                     /locus_tag="WJL14_001576"
     CDS             complement(1159..2076)
                     /gene="hisB"
                     /locus_tag="WJL14_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ7656627.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            complement(2073..3170)
                     /locus_tag="WJL14_001577"
     CDS             complement(2073..3170)
                     /locus_tag="WJL14_001577"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ7656628.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFSSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            complement(3216..4505)
                     /gene="hisD"
                     /locus_tag="WJL14_001578"
     CDS             complement(3216..4505)
                     /gene="hisD"
                     /locus_tag="WJL14_001578"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ7656629.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            complement(4502..5401)
                     /locus_tag="WJL14_001579"
     CDS             complement(4502..5401)
                     /locus_tag="WJL14_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ7656630.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            complement(5505..6158)
                     /locus_tag="WJL14_001580"
     CDS             complement(5505..6158)
                     /locus_tag="WJL14_001580"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ7656631.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            complement(6237..6890)
                     /locus_tag="WJL14_001581"
     CDS             complement(6237..6890)
                     /locus_tag="WJL14_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ7656632.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            7167..7251
                     /locus_tag="WJL14_001582"
     tRNA            7167..7251
                     /locus_tag="WJL14_001582"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7201..7203,aa:Leu,seq:caa)
BASE COUNT         1503 a         1749 c         2139 g         1919 t
ORIGIN      
        1 cttcgtcgcc ttgtcctgat ttttgttaat ccactatata gtcataatcg ggactgattc
       61 caagcgtggg gtgatatagt ggattaaatt taaaccagta cagcgttgcc tcgccttgtc
      121 ctgatttaaa tttaatccac tataaaaatg ccgtctgaaa tatgttcaga cggcattgtg
      181 tttggaaaag gcaatcagtg ttctgccaat ttcaggtaaa cgccggaaac gtcctgttcg
      241 ccgtagccgg cttcgactgc tttgcggtag ctggcagcaa cggtttcgac ggcgggcagg
      301 gtgttgcctg cctgttcaag ctctttgacg gcgaggttga ggtctttgga ggcgtgtttg
      361 agggcgaagg cgggtgggaa ttcgcggttt gcccacaggg attttttggt ttggaacatg
      421 ggcgagtcca ttgccgagcc gccgatggct tcgacgatgg tgtcggtatc gatgccgaac
      481 tgccgcgcca tcagcatcgc ttcgctgtac gcttcgccga aaatgcccaa gagcgagttc
      541 aagacgagtt tcgcgcccga acctttgccg acatcgccga aatggaaggt ttttttgccg
      601 acgagggaaa atattttttg cagcgggttt aaaacggctt cgctgccgcc gaacagaatc
      661 agcagcgtgc cgttggtggc gggcccgacc gatccggaaa cgggtgcttc ggcaaactgt
      721 ccgcctgcgg cttcgacaag tgctttgacg gcgaggtttt cggtcgggga gatggtgctc
      781 atgttgacga tgattttgcc ggccaatccg tcgcggactc cgttcaggat gtcgcacacg
      841 gcggcatagt cggaaaccat caggaaaatg acgggatagt cgcggacgag ttcggcggtg
      901 ttgccgtaaa cttttgcgcc tttggcggag atgggggcag ttttgtcggg cgagcggttg
      961 tatacgccga cttcgatgcc gccgtccaag agccgcgtta ccataggcag acccatttgc
     1021 cctaagccta tccagccgat ttgtgcgtat tcgtttgcgg acatgatgtt tactcctttt
     1081 ctgtttgtag tgtagtgtaa tcttatctgt tccgataccg tctgaaaagc ctgcgggtgt
     1141 ttcagacggt atggtttttt atgcggtcag cgtgcctttg gtcgaggggg tctgtcctgc
     1201 catgcgcggg tcgtgttcga ctgccatacg cagggcgcgc ccgaaggctt tgaatacggt
     1261 ttccgcctga tggtgggcgt ttttgccgct gaggttgtcg atgtgcaggg tcatcatact
     1321 gtggttgacg atgccgtgga aaaattcttc aaacaaatcg acatcgaaac gtccgattag
     1381 tgcgcgggta aattcgatgt tgtacacgag tccggggcgg ccggaaaggt cgatgacgac
     1441 gcggctgagg gcttcgtcga gcgggacgta ggaatgtccg taacggcgga tgcctttttt
     1501 gtcgccgagt gcctgccgga ttgcttgtcc gagtgtgatg ccgatgtctt cggcggtgtg
     1561 gtggtcgtcg atgtgcaggt cgcctttgca gctgatgtcg atgtcaatca tgccgtggcg
     1621 ggcgatttgg tcgatcatgt gttcgaggaa gggtacgccg gtatccagcc tgcttttgcc
     1681 gctgccgtcg aggttgatgg agacggtgat ttgggtttcg caggtattgc ggttgacggt
     1741 aacgctgcgg cagccggcag cagatgcggt ttcggcaatt tgggtttcgg cagttccggt
     1801 gctttctgcg gcggtttcgg ggacagtgcg ttcgcggtgt tttctgtcga gccagccttt
     1861 gggtttgccg gtgtgttttt cgagttttgc catcaggctg ggacggatgc cgcgtgcatc
     1921 tgggtcttct gcctgctttt caaggcgttg tttgagtttg tagagtgcga cgggggtacg
     1981 gtagccgcag agtttggcga gcttggacag cgaacctgct tcttgggcga gggtcagaaa
     2041 gttgtgcagt tggcgttgtg ttttagtcaa attcatagga aatccgttgg ttggcagtaa
     2101 agttggcgaa tgatgttgag aacggcatcg ttttgtgcgg ggctgcctac ggtaatgcgc
     2161 aggcagtgtt ccaaaagcgg gtgcgcgcca tgcagttttt taaccaagat gcggttttgt
     2221 ttgagcgtgt caaacaacaa atcggcatcg ggtacgcgta tggtaatgaa gtttgcctga
     2281 cttgaaaagg tgttcagacg gcatattttg cccaattcgg cgaacatccg ttcgcgttcg
     2341 tttttcaggc tgtcgatgtt ggcagagatg atgccgtagt gttgcagggc gagtttggcg
     2401 gtggtcaggc tcagttggtt catattgtag ggcggcagga ttttttgcag ttcgccgatg
     2461 acttcgggac agcctgccgc atagccgata cgcagtccgg caaaaccgat tttgctgacg
     2521 gtacgcatga cgatgaggtt gggaatgctg cccgcctgcg gcaggaagct gtcgccgttg
     2581 aatgcgccgt aggcttcgtc gacgacgacg atgccgtctg aagcttcgat gacggcttcg
     2641 atttcggcac gcgtgaaaca tacgccggtg gggttgttgg ggtaggcgat aaaggtcagg
     2701 gcagggcggt gcttcctgac ggcttcgagg acggcgggca ggttgagggt gaaatctccg
     2761 ttcagtggaa cgccgacata atccatgccg tacagcgcgg cgttgtggcg gtacatgacg
     2821 aaactgggtt cggctgccaa cattgccgcg cccggtttgg cggtcagcat cgtgatgaac
     2881 tgtatcagct cgtccgagcc gttgcccagc gcgatgtcgg cgcagtcggg aatgtcgaac
     2941 gccgaacgta atgcttcctg taaaccgctg ccggagggat tggggtaaag atggatgggc
     3001 gcggcggcaa gccgtgcctg ccattcctgc aacagcgttt catgtccggc aaaagggtgg
     3061 acgggacttt ccatcgaatc gagtttggca aagccgggcg gaacgtcggc aatctgatat
     3121 gccgacatag cttgtatgtc gtcgcggatg aaggagcgga cggatttcat cgtgtttcct
     3181 taatggttgg aatatgcctg tacgccgttt cggcattatt tcatacggaa ctctgccgcg
     3241 cgggcgtggg cggtcaggct ttcgccgtgt gccagcacgc tggcggtttc gcctaatttt
     3301 tgcgcgccct gttccgaaac ctgaatcagg ctggagcgtt tttggaaatc atatgtcccc
     3361 aaaggcgagg aaaagcgggc agtgcggctg gtgggcaata catggtttgg accggcgcag
     3421 tagtcgccga ggctttcgcc ggtgtagcgt cccatgaaaa tcgcgccggc gtggcggatt
     3481 tttttcgccc attcctgcgg attttcgact gacaattcca agtgttcggg ggaaatgtag
     3541 ttggcgattt cgcaggcttc gtccaagtct ttggcgagta tcatcgcgcc cctgttgccg
     3601 agcgaggctt cgatgatgtc gcggcgcggc atggtttcga tgagcctgtc catagcggct
     3661 tctacttcgt cgagatacgc ttgcgacgtg ccgattaaga tggcttgggc aatttcgtcg
     3721 tgttcggcct ggctgaacaa atccatcgcc acccaatcgg caggtgtcgt gccgtcggcg
     3781 atgaccagga tttcagacgg ccccgccacc atgtcgatgc cgaccacgcc gaacacgcgg
     3841 cgtttggcgg cggcgacgaa ggcgttgccc ggaccggtga ttttatcgac ttgggggatg
     3901 gtttccgtgc cgtaggcgag ggcggcaacc gcctgcgcgc cgccgacggt gaagactttg
     3961 gttacgccgg cgacgtatgc ggcggcaagc acgatgtcgt tgcgttcgcc tttcggtgtc
     4021 ggcacgacca tgatgatttc tttcacgcct gcgacgtggg cgggcatggc gttcatgatg
     4081 acggaactcg gatatgccgc cttgccgccg gggacgtaaa tgccgacgcg gtcaagcggg
     4141 gtaatctgtt gtcccaacag cgtgccgtct tcatcggtgt agctccacga ttccattttt
     4201 tggcgttggt ggtagctttc gacacggcgg gcggcggtct gcaatgccgt ctgaacgtcg
     4261 ttcggaatgc gctcgaacgc cgcgttcaaa tcggcttgcg tgagtattaa atcatcgatg
     4321 cttttagcgt ttgtctgatc gaatttgttg gtgtattcaa tcaaagccgc atcgccgcgc
     4381 ttttgcacgt cggcgcaaat gtcggcgacg atgcgttcgg tttcggggtt ttgcgcggtt
     4441 tcaaaagcca gcagggcttt gagtccggct tggaaatcgg gcgattgggt gttgagtttt
     4501 ttcatgattt ggttttttcc ttttttattt ttggattggg ggtcgtctga atattctctt
     4561 gttcagtttc tgtctctgtt ttgggtctga tatacgcttt ttctcccccg ctaccaaact
     4621 tcaattcggg caggcatttt tgttgcagac cgagtttggc taatacctct tcgctacgag
     4681 aggtagtgcg gaaacggtaa cgggattgtt ttctaccgtt tttggtttcc tcaaccattg
     4741 tttcaaaacc gtccacttcg tatgcactga caacagcgtt ttgcagaccg gtgtgaacgc
     4801 cgataacgta tttcactttt gaagcaacat ctttaccgat aacccagttg cccaacgtac
     4861 gcgatttgag gttggcatcg tcttggttgt cgaaaaggta gtctaattct tcgtcagtat
     4921 ctaaatcgaa agcattgtgg attttgatgg cgagaatcag cccgtcgggg ttaatctcgt
     4981 taagtgggag agaagagaat ccaaagcggc gatctagttc ttctacgcta ataccacccg
     5041 gaccatgccc ggcaatttta tttttgagtt ttttacccaa gacagattta acaaaatgaa
     5101 ttaaggcaga ttcggcggcg agtgcttcga cttcagtcag atgatagctg atgatatagc
     5161 gaccgagttt cttgcatttg gagatggctt tgagtttccg atcgataatc tcgccggaga
     5221 ctgggtcttg tgaacgacta gcaacccatt catgctcgaa gatacgatta ccgcagcctt
     5281 tgccgatgta gaggattttg tcttttttca ggtcggtcag acaatagaca taaaaccgac
     5341 gctcgccgtt attcaaaacg gaaagggttg aatctgagaa tttttttatt tttgcaacca
     5401 ttttaacttc tcctatacag tttactgccg gtgatatttt cagacgacct gtcggcggga
     5461 acggataggg tcgtctgaaa acgcatcttt attcaaatgg atgcttactt cgccttcact
     5521 gcgccgccga acgcctgaat aatcggctcc agcagcgcgt atttcgtttt caaagcagcc
     5581 ttgttgacca ccaggcggct ggaaatgtcg acgatgtgtt cgactgcttc caagccgttt
     5641 gccttcaagg tgttgcccgt cgaaaccaag tccacaatcg catcgctcaa gccgaccagc
     5701 ggcgcaagtt ccatcgagcc gtacagtttg ataatgtcca catggacacc cttgccggca
     5761 aaatgagatg ccgcgatttc aggatacttc gtggcaatct tcagacggca tccgggttgc
     5821 gaagctgctt cgtaatcaaa ccctttacgc acagcaacca tcatgcggca cttggcaatc
     5881 tccaaatcca aaggccggta aagccccgtg ccgccgtgtt cgatcagcac gtctttgccc
     5941 gcaatgccga agtccgccgc gccgtagcgg acataagtcg gcacatcggt tgcgcggaca
     6001 atgacaaggc ggatgttttc atggttcgtc ccgataatca gcttgcgcga tttttcaggc
     6061 tcttcagtcg gaacaatgcc ggcagcggca agcagcggca gcgtctcctc aaaaatgcgc
     6121 cccttggata aggcgatggt caaagcatta tcctgcatgg tgtggatgtc ctttgtcggt
     6181 tgaaacaata atgccgtctg aataggcgcc gacggtgcag acggcatacg gttttatcag
     6241 agcagagtcc ttacatcggc agcaatcgtt tccggctcgc ttccgtaagg cgagaaaatg
     6301 gcaacctcac cgtttttgtc gatgagatac gcaccggaag agtggtcgac caaatagttt
     6361 tcgctgtcgt ctttttgatt gactttggca gaaaccacgc ggtattgctg cttgatgacc
     6421 ggcaggtttt ggtcgcccgt tgccgtcaga ccgataaagt ccggattgaa ctgtttggca
     6481 tacttgccga tgatttcagg cgtgtcgcgt tccggatcga tgctgacgaa caccactttc
     6541 acgtccttag cctgcccgcc caactgcttc aacgtgtcgc tgtacgtcaa aagctctgtc
     6601 gggcagacat cgggacagtg cgtaaagccg aaagacagaa tcacgacctt gcctttcaaa
     6661 tcgctcaggc tgaaaggctt gccttcgccg tcggtcagcg tgaaatcgcc gccgatgtct
     6721 tccttacgca tatccgtacc gcgcgtttgc ggctttgccg cattttccgc agctgacgcg
     6781 gatgcacttg aagaagcggc ttgcgccgca ctgttgtctt gaggtttgca ggcggcaagc
     6841 gcggcaagta cgaaaacgcc cggcaaaaag gaacgcggta cggaaaacat gacaatctcc
     6901 caagaataaa tagcaaaacc tgattcgtac ggaatttgaa tatttccatc ttaacgcgtt
     6961 tggcagattt aatgtagtat ttttttacgt tgtttgcacg gcaggaattg ccgaatcata
     7021 cagaatcttt ggagcggaaa gaacagtaag attaaaataa taaaaattct atttaaaatc
     7081 aaaaataaaa taaaaacaga taaaataatt tcaaaaaaaa ggtttacaga atttcaaaag
     7141 ccattataat gacgactcct tcaccagccc aggtggcgaa attggtagac gcaggggact
     7201 caaaatcccc cgccgcaagg tgtgtcggtt cgagtccgac cctgggcacc acaaccgctt
     7261 ttagagcggt tatttttttg tctattgctg tttaatgaaa tagataaaac
//
LOCUS       DAWKQB010000105         7302 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.105, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000105 DAWKQB010000000
VERSION     DAWKQB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.105"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(58..480)
                     /locus_tag="WJL14_001583"
     CDS             complement(58..480)
                     /locus_tag="WJL14_001583"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ7656633.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(491..1888)
                     /gene="atpD"
                     /locus_tag="WJL14_001584"
     CDS             complement(491..1888)
                     /gene="atpD"
                     /locus_tag="WJL14_001584"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ7656634.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(1926..2801)
                     /gene="atpG"
                     /locus_tag="WJL14_001585"
     CDS             complement(1926..2801)
                     /gene="atpG"
                     /locus_tag="WJL14_001585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ7656635.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYQNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2826..4373)
                     /locus_tag="WJL14_001586"
     CDS             complement(2826..4373)
                     /locus_tag="WJL14_001586"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ7656636.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEHEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4384..4917)
                     /locus_tag="WJL14_001587"
     CDS             complement(4384..4917)
                     /locus_tag="WJL14_001587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ7656637.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(4922..5392)
                     /locus_tag="WJL14_001588"
     CDS             complement(4922..5392)
                     /locus_tag="WJL14_001588"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ7656638.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5463..5699)
                     /gene="atpE"
                     /locus_tag="WJL14_001589"
     CDS             complement(5463..5699)
                     /gene="atpE"
                     /locus_tag="WJL14_001589"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ7656639.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5756..6622)
                     /gene="atpB"
                     /locus_tag="WJL14_001590"
     CDS             complement(5756..6622)
                     /gene="atpB"
                     /locus_tag="WJL14_001590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ7656640.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6612..6965)
                     /locus_tag="WJL14_001591"
     CDS             complement(6612..6965)
                     /locus_tag="WJL14_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ7656641.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1919 a         1942 c         1660 g         1781 t
ORIGIN      
        1 aacttggaaa aataaaaagc acgaccaaac ggtcgtgctt ttccaaacca aacaagttta
       61 tttcttgtgc gaacggatat agtccaaagt tttgagctgt gcaatcgcag cagccaatgc
      121 tttatgcgcc tccgccaaag ccttatcgtc tttagcttgg gaaatgcccg cttctgcggc
      181 ttttttcgcc tcttccgcac gtgcctgatc catctctgca ctgcggacgg caacatccgc
      241 caagacagtt actttatcag gctgtacttc caaaacaccg ccggaaacag caaccaaaac
      301 ctctttatcc tcgcccggaa cggtcaaacg caaagccccc ggccgcacca aactcataat
      361 cggctcgtgt cgcggataaa taccgagttc gccctgtaca gtcggaacaa cgataaatgt
      421 tgcctcgcct gaatagattt tctgctcgct actgaccacc tcaacttgca tgatgctcat
      481 gccgacctcc ttagtttaag gttttcgctt tctctactgc ttcttcaatg ctgccgacca
      541 tatagaatgc ctgctcgggc agatgatcgt attcgccgtt caagatggct ttgaagccgg
      601 caatggtatc gcgcagggcg acatatttac ccggagaacc tgtaaacact tcggcaacgt
      661 ggaacggttg ggacaggaag cgttggattt tacgcgcacg cattacggtc agtttgtctt
      721 catcagacaa ttcgtccata cccaagatgg cgatgatgtc gcgcaattct ttgtatttct
      781 gcagggtgga ctgtacgccg cgcgctacgt cgtagtgctc ttgacccaat accatcggat
      841 ccagttggcg cgaagtagaa tcaagcggat cgactgccgg gtaaataccc aaagaggcaa
      901 tatcgcggct caatacgacg gttgcgtcca agtgggcgaa cgttgttgcc ggagacgggt
      961 cggtcaagtc gtccgcaggt acatatacgg cttggatgga agtaatggaa ccggtttggg
     1021 tagaggtaat acgctcttgc aaacgaccca tttcttctgc caatgtcggt tggtagccca
     1081 ctgcagacgg catacggccc aacaatgcgg atacttcggt accggccagg gtgtaacggt
     1141 agatgttgtc cacgaagaac aatacgtcgc ggcctttgcc gttttcgtct ttttcgtcac
     1201 ggaagtattc cgccatggtc aaaccggtca atgcgacgcg caaacggttg cccggaggtt
     1261 cgttcatctg accgtaaacc attgccactt tatccaatac gttggaatct ttcatctcgt
     1321 ggtagaagtc gttaccttcg cgggtacgct cacccacgcc tgcgaacacg gacaagccgc
     1381 tgtgcgcttt ggcgatgttg ttgatcaatt ccatcatgtt cacggtttta cccacaccgg
     1441 caccgccgaa cagacctact ttaccgcctt tggcaaacgg acacagcaag tcaatcactt
     1501 taatgcccgt ttcgagcaat tcggttgtgg aagacagttc gtcaaactta ggagcggctt
     1561 ggtggatggc acgactcttg tcggtatcga tcggacctgc ctcgtcaaca ggcgttccca
     1621 atacatcgac aatgcgtccc aacgtacctt tacctaccgg cacagtaatg ggcgcaccgg
     1681 tattgctcac agtcatgccg cgtttcaaac cgtccgagct gcccatcgca atggtacgga
     1741 ctacgccgtc gcccaaaagc tgttggactt ccaaagtcag accgttttcg tctaatttca
     1801 aagcgtcgta aacgcgcgga atcatgtcgc gtggaaattc cacgtcaaca accgcaccga
     1861 taatttgtac gattttgcct tggctcatta tcgtatccta atttccgtac aggattcaga
     1921 cggcatcaga cagccgccgc acctgctaca atttctgaca attccgtggt aatcgcagct
     1981 tgacgcgatt tgttatatac caaacgcaac tctttgatgg cattgcctgc attgtctgtt
     2041 gcagctttca tggcaaccat gcgggctgcc tgttcggatg ccatattgtc gctcaacgcc
     2101 tgataaacca cagactctaa atagcggcga accaaatatt ccaacactgc aagtgcagtc
     2161 ggttcgtagc ggtattccca gctgaacggt gatttgggag ctgaatcgcc aatcacgttc
     2221 tcaccgatag gcagcaatac ttccattctc ggttcttgac gcatggtatt gacaaaaccc
     2281 gaatacacca gatggattct gtcaatttca tgtttctcat accgttggaa gagttctgtc
     2341 aaaggtccga gcagcatttc catttttggg gtatcgccca aatttacggc actggcaacc
     2401 acattcagac caatgctctg acacgccatc agacctttac tgccaaggca tacgatttcc
     2461 tcttcaatac cttgattctg atactcttga acttgtgcca aaaacttttt cagcacgttg
     2521 gcgttcaaac cgccacacaa acccttatca gacgtaatca aaataaaacc gacacgtctg
     2581 atttcccgat gagattccag taacggaata ccatgatcgg tattggtttg cgcaagatgg
     2641 ctcatcacca tacgcacttt ttcggcatac ggacgcgcca aacgcatccg ttcctgagtc
     2701 ttccgcattt tagaggttga caccatctgc atcgctttag tgatcttttg ggtattctga
     2761 acactgcgga ttttggtgag aatctctttt cctactgcca tttcagactc ctttcacttc
     2821 aagccttatg cctgataggc gtaagaagat ttgaaggatt tcatggctgc ttcaagcgtt
     2881 ttctcgctct cgtcggacat tgcacctgaa gcattgacgg cttccaaaac ttccggatgt
     2941 tgggtacgga caaagctcaa aaattcagat tcaaaagcca gagctttggc aaccggaaca
     3001 tcagaatacg aaccgttgtt gattgcccaa agggtcaaag ccatttcagc cgtattcaac
     3061 gtactgaact gtttctgttt catcagttcg gttacgactt cgccatgctc caattgtttg
     3121 cgcgtagctt catccaaatc ggatgcaaat tgcgagaacg ccgccaattc acgatattgt
     3181 gccaacgcca aacggatacc gccacccagc tttttaatca ctttggtttg tgcagcaccg
     3241 cctacgcggg atacggaaat accggcattg attgcaggac ggataccggc gttgaagagg
     3301 tcggtttcca agaaaatctg accgtcggta atcgaaatga cgttagtcgg aacgaaagca
     3361 gatacgtcgc ccgcttgggt ttcgataatc ggcaaagcag tcagagaacc ggttttgcct
     3421 tttacttcgc cgttggtcaa tttctccact tcgtgttcat tgacacgtgc tgcacgttcc
     3481 aacagacggg agtgcaggta gaacacatcg ccgggatagg cttcgcggcc gggcggacgg
     3541 cgcaaaagca gggaaatttg acggtaagcc acagcctgtt tggacaaatc gtcataaaca
     3601 atcaaggcat cttcgccgcg gtcgcggaag aattcaccca tcgtacaacc ggagtaaggt
     3661 gcgatatatt gcaatgccgc cgcttcagat gcagttgcag caaccacaat cgtgtgttcc
     3721 atcgcgccat gctcttccaa tttgcggacc acgttggcaa tagaagatgc tttttgaccg
     3781 atagcgacat agatacagat aacacccgta cctttttggt tgacgatggc atccagcgct
     3841 acggccgttt taccggtttg acggtcgcca ataatcaact cacgctgacc gcgaccgaca
     3901 ggaaccatag agtcaatcgc cttcagaccg gtttgcatcg gctggtcaac cgatttgcgc
     3961 gcaatcacgc ccggtgcgat tttttcgata ggggcggtca aagttgtatt aatcgggcct
     4021 ttgccgtcga taggccgacc caatgcatca acgacgcgtc cgaccagttc gcgtccgacc
     4081 ggcacttcca agatacgacc ggtacaggta accgtgtcgc cttctttaat atgttcgtgc
     4141 tcgcccaaca ctacggcgcc gacggagtcg cgctccaggt tcatcgccaa gccgaaagtg
     4201 ttacccggga attcgagcat ctcaccttgc attgcatctg acaaaccatg gatgcgaacg
     4261 ataccgtcag ttaccgaaat caccgtacca cgggtacgca cttcggcatt tacagacaga
     4321 ttttcgatct tggctttaat caaatcgcta atttcagcag gattaagctg catgaaaact
     4381 ctcctaattc gtcatagtcg tgtacaaggc actcaatttg ccttgtacag acaaatccaa
     4441 aacctgatca cccacttcaa cttttatgcc gccaatcagc tccggttcga tttcgacaga
     4501 gattttcagc tcgctgtcga aacgcttatt cagcatttgc gccaactcgc cgacctgttt
     4561 gtcggtcaac ggataggcac tgtaaatgac ggcagatttg atatggttga atgataaggt
     4621 caagtcttga tattgagcat atacttccgg caatatcgac aaacgtttct gcccggccaa
     4681 gacgataaca aagtttttca actccttgtc tttcaaaccg accaaatcga tgaggatatc
     4741 tgctttttct gaagcattcg tttcaggacg gtcaatcaat gaagccacct tcccttcctg
     4801 aacaaccgcc gcaagttttt ccagtccgcc caaccaagac tcaatttggc ttttttcctg
     4861 agccagaccg aacaatgcct ttgcataagg tctggcaatc gttgcgaact ctgccataag
     4921 attacagctc ctgtttcagg gtatcgagca gttttgcgtg tttggaagca tcgacttcgc
     4981 tgcgcaaaat agattcggca cctttgacag ccaacacggc aacctgctcg cgcagggatt
     5041 cgcgtgcgcg gaacaattcc tgctccacat cggcctttgc ctgagctgca atgcgcgccg
     5101 cctcggaaga agcctgttct ttggcttctt cgacaatttt ggcggcacgt ttttcggcgt
     5161 tggcaaccat ttcggaaacc tgattacgcc cttctgccaa gagttctgca accttttttt
     5221 cagcctgctc gaaatcgctt ttaccacgct cggcagctgc caagccttcg gcgacttttg
     5281 cggcacgctc atccaaagct tttgcaatcg gcggccacac gaatttcatg gtaaaccata
     5341 ccaaaccgaa aaagacgatg atttgagcga ataatgttgc attgatattc acgttactta
     5401 accttcgtat tggggttaat caaacaggct gcgcctgtac ggaacggacg aatccgtcct
     5461 gattatgcac ctgcaaacgg gttaacgaag gcgaacagca gtgcaatggc gacaccaatc
     5521 aagaatgcgg catcaatcaa accggcaatc aggaacagtt tggtttgcag cggaccgatc
     5581 agttcgggct gacgggcaga agactccaaa tatttagaac cgaccattgc gataccgata
     5641 gaggcaccca gtgcgcccaa tgcaacgatc aaaccacatg cgatagcaat caaacccatt
     5701 ttaaactcct taaagaaaca aaggttaaac tacaaaaaca aactacttag gaaaatcagt
     5761 gcgcatcatg tgcctgtccg atatagacga acgccaacgc catgaaaata aacgcctgca
     5821 gggtaatcac caaaatatgg aaaatcgccc atgccaaacc ggcaataatg tggaatacaa
     5881 acagaatcgg atccatgact tcgacgctgc cggaagccgc ccaagcaccg ccaagcaagg
     5941 ctatcaacaa gaataccaac tcgcctgcat acatattgcc gaacaaccgc ataccgtggg
     6001 atacggtttt agaaagaaac tcgaccaaat tcagcaggaa gttcgcaggt gcgagttttg
     6061 caccgaacgg cgcgctgaac aactcgtgaa accatccgcc caatcctttg attttgatgt
     6121 tgtaatagat acaaatcagc aacacgccga cagcgagtgc caaagtggtg ttcaaatcgg
     6181 cagtcggtac gacgcgcaac agggcgtgat ggttgccggt aatgccctgc cataccatcg
     6241 gcagcaaatc gaccggcagc atatccatcg cgttcatcag gaaaatccag acaaacagcg
     6301 tcagacccaa cggcgcgacg gcttttctag acttttcgtt gtgaatgatg ctcttacaca
     6361 tatcgtccac aaactcaaac aagatttcca cggcggcctg gaaacgtccg ggaacgcctg
     6421 ccgtcgcttt ttttgcaccg cgccacaaca ggaagctgcc gattacgccc aacaggacgg
     6481 caaaaaagac ggcatcaagg ttaataaacg aaaaatcagc aatgtttttc agtccctgac
     6541 cctgagtaac atccgacaaa ctggtcaagc tctgcaagtg gtgcttgatg tagtcggcag
     6601 cggtaatggt ttcacctgcc ataatctttc actctcaaca atactaaaaa aaccaaatgg
     6661 ctgacaccga gcagccccat cagaaacggg gcgaacacca gcgattgatg ccatacagca
     6721 aatacggcga gcatggacaa cagcgacagc actactttta aaatctctcc gaagacgaac
     6781 atcctgcttt gcaggaaggg gtttcccctg aaaagtttta aaagtaaaac tgcaacaaac
     6841 gtgggaagca ggtaggacaa accgccaccg accgccgaaa ggaatccggc aaaaccccat
     6901 acggcaaagg caactgcggc gcatatggac aatacggcgg attgtaggat gataatctgc
     6961 ttcataaagg gaatgtttcc gcctcgaatt tgaggcgcgg ctaatataat ttagaagcct
     7021 tattacgtca agcaatatgt taatctttgt gaaacaacgt atcccaatcc gccgcgctcg
     7081 ccgcctgaat aacggcgaca ggtgtcattc taacacacat tacatataat tacaggatat
     7141 taaggagttt gtccgcaatt tctttacact tttaatgttc ttacgtgatt tgtttgcttt
     7201 acgtggaaat aataaaaaat caacgcgaaa ttgtagcagt ttatcggtcg gattgtcggc
     7261 agtttgggga atttgctcaa taaataaaag gtcgtctgaa aa
//
LOCUS       DAWKQB010000106         7244 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.106, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000106 DAWKQB010000000
VERSION     DAWKQB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.106"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(304..1563)
                     /gene="rho"
                     /locus_tag="WJL14_001592"
     CDS             complement(304..1563)
                     /gene="rho"
                     /locus_tag="WJL14_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ7656642.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WJL14_001593"
     CDS             complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WJL14_001593"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ7656643.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4579..5400
                     /locus_tag="WJL14_001594"
     CDS             4579..5400
                     /locus_tag="WJL14_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ7656644.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5640..6302)
                     /locus_tag="WJL14_001595"
     CDS             complement(5640..6302)
                     /locus_tag="WJL14_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ7656645.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6315..7142)
                     /locus_tag="WJL14_001596"
     CDS             complement(6315..7142)
                     /locus_tag="WJL14_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ7656646.1"
                     /translation="MKPDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNRWYQHLCRE
                     RGLEPIALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQSLKQNKNTV"
BASE COUNT         1502 a         1739 c         2152 g         1851 t
ORIGIN      
        1 aggcaactct aacttaattt gaatttccct agttatctag gacagcccca aatttataca
       61 aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt tcattgccgg
      121 tacggatgcg ttcaagccgg ttttgcaaag gccgcgcttt cggcaagcgg acacggacac
      181 tgccggcggt tgcgccgtta acggggggag ggaggagctg cgccggccgt gtgaatgaaa
      241 gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat gcgggtggcg
      301 cgtttatttg ccgcgcatca gttcaaagaa atcatcattg tttttagagg ctttgatttt
      361 cccgattaaa aattcggctg cctcgatttc gtccatcggg tgcaggaact tgcgtaagag
      421 ccacatacgt tgcaactggt cgttcggcac aagcagctct tcgcggcgcg tgccggattt
      481 gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcaa ggtgcaattc
      541 catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc tgccggtttc
      601 aaccaatgcg gtggcgatga tggtcagcga accgccttct tccacgttgc gcgccgcgcc
      661 gaagaaacgt ttggggcggt gcagtgcgtt ggcatcgaca ccgccggtta ggattttgcc
      721 cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat ccagcaggat
      781 gaccacgtct tttttgtgtt ctaccatacg cttggctttt tcaagcacca tttcggcaac
      841 ttggacgtgg cgttgcgccg gttcgtcaaa ggtggaggaa accacttcgc cgcggacgga
      901 acggctcatt tcggttactt cttcgggacg ctcgtcaatc aagaggacga tgagttcgac
      961 ttcgggatag tttgaggtaa cggcgtgggc aatgttttgc agcatcacgg ttttaccgct
     1021 tttgggcggg gcaaccaaga gggcgcgctg acctttgccg ataggggaaa tcaggtcgat
     1081 ggcacgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca actgttcggt
     1141 cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt ccgggtggtc
     1201 gccgttgatg gtatcaagcc tgaccagggc aaaatagcgt tcgttgtctt ttgggacgcg
     1261 cacgcttcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt gggtgggcga
     1321 gacatagatg tcgtcggggc cggcaagata ggacgtgtcc gcgctgcgga ggaagccgaa
     1381 gccgtcgggc aggatttcaa gcgtgccgga gcaggtgaaa ccctcgcctt ttttcatcat
     1441 ctggcggacg atggcaaata cgagatcttg tttgcggaat cggttggcgt tttcgatgcc
     1501 gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt cggagacgtg
     1561 cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg aaagaagaag
     1621 ctgaccgttg ccggttgctc ggggaagggg gaattgtagg cagtcggcgc gtgggtgtca
     1681 aatattatcg cggacggggc atcggcagga aatgccgtct gagcggagct gcttggaaaa
     1741 aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt tcaattcatt
     1801 cgccaaatat agccaagttt cgatgacggt atccgggttc agggaaacgc tttcaatgcc
     1861 ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac cgcagatgcc
     1921 gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca ctttgacggc
     1981 agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga gaccgagggt
     2041 cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca ggaattgttc
     2101 cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc cgtttttgcc
     2161 gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc ccaaagtgcg
     2221 gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga cgcgtttcaa
     2281 ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac gcgccgcacc
     2341 acggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc cgaccaggtt
     2401 ggcgtattcg ttggatttga agtcggacat acggacgatg gttttacgcg gataaaccga
     2461 tgcggccaat gtcgccacgc cttcggcgat tttatcgacg tagaagtcga caggggacgc
     2521 gtaaccggcg atacggcggg taatttccgc ttttaattcg tcgtcttgtt tgtcaaattc
     2581 caacaaggct ttggggtgga taccgatttg gcggttgatg ataaattcca tacgcgccaa
     2641 gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcgggat tgccgacgtt
     2701 catcatgact tttacaggtg ctttaggcat gttgtccaaa gcaacatcgg taatttgtac
     2761 gtccagcagg ccggagtaga tgaagccggt atcgccttcg gcacaggata cggtaacttc
     2821 ttgaccgttt ttcagcaatt cggttgcatt gccgcagccg acaacggcag gaatacccag
     2881 ttcgcgcgcg atgatggcgg cgtggcaggt acgtccgccc ctgttggtca cgatggcgga
     2941 agcgcgtttc atcaccggtt cccaatctgg gtcggtcatg tcggtaacca gtacgtcgcc
     3001 ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgtaccttgc cctgaccgac
     3061 tttctgaccg atggcgcggc cttcgcacaa gacggttttt tcgccgttga tagaaaagcg
     3121 gcgcaggttg cggctgcctt cttcctggga tttgacggtt tcgggacggg cttgcaggat
     3181 gtagagtttg ccgtccaagc cgtcgcgtcc ccattcgatg tccatcgggc ggccgtagtg
     3241 tttttcgatg gtcagtgcgt aatgcgccaa ctcggtgatt tcttcgtcgg taatggagaa
     3301 gcggttgcgg tcttcttcgg ggacatcgac gttggttacc gatttgccgg cttctgcttt
     3361 gtcggtaaaa atcattttga tgtgttttga gcccatggtt ttgcgcagga tggcaggttt
     3421 gcctgctttc agcgtgggtt tgaacacata gaattcgtcg ggattgactg cgccttgtac
     3481 gacgttttcg cccagaccgt aggatgaagt gacaaagacg acttggtcgt aaccggattc
     3541 ggtatcgagg gtgaacatca cgccggacgc gccgctgtcg gaacgcacca tacgctgcac
     3601 gccggcggaa agggcgacga tgtcgtgttc gaagcctttg tggacgcggt aagaaatggc
     3661 gcggtcgttg tacagggagg cgaatacatg gcgcatcgct tctttaacgt tttccaagcc
     3721 gttgatattc aggaaggttt cctgttggcc ggcaaatgat gcgtcgggca ggtcttcggc
     3781 ggttgcggaa gagcgtacgg caacggaaat gtccgcgccg ccggcatcgg caaccatttt
     3841 gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aagggcgtgt ccaaaatcca
     3901 ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat ctaattttgc
     3961 cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc ggtaggcatc
     4021 ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct gactgatcat
     4081 ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac gcaggttttc
     4141 aaaccaaatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg ttaaaaaaga
     4201 aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg ttttatccgt
     4261 tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg gtttgaaact
     4321 tgcagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg tttgaatagg
     4381 ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc caaaaaatac
     4441 tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta atccgaggta
     4501 ggtgtgctgc gagtgctttc cttgtgtctg ccgctgctgt tatgatggga ttttaaacct
     4561 gtgttttaag gatggaagat gagcagtccg cgccatgtgt tttacatttc cgaccgtacc
     4621 ggtctgactg ccgagaatat cggcgaggct ttgctgaacc agtttggcaa tctgtcgttc
     4681 aaacgccata cgcatccgtt tgtcgatacg ccggaaaagg cgcgcgcggt ggtggagaag
     4741 gtcaatcgga gccggcagga aaacggtcag cgtccgattg cgtttgtcag tgtggttgat
     4801 gacgaaatcc gtcggattat caaaggggcg gatgcttttc agattaattt ctttgagact
     4861 tttttgggac tgttggagaa agaactcaat accgaagcca cggcatccga gcaggggcat
     4921 cacagtatcg gtaatacgaa gcgttacgat gcgcgtatgg aggcggtgaa tttctcattg
     4981 aaccacgatg acggggtcag cgataagaac cttcaggaag cggatgtaat cttgatgggt
     5041 gtatcgcgtt cgggcaaaac gccgacctgc ctttacctcg ccctgcaata cggcatccgt
     5101 gcggcaaact atccgctgat tcccgacgat ttggaatcgg ccgatctgcc gcgtatggtc
     5161 aagccttata gggataagct gttcgggttg accatccagc cggaacgttt gcaggccatc
     5221 cgccaagagc gccgcccgaa ttcaacttat gccaaaatcg atacatgccg cagcgaggtg
     5281 gcggacgcgc agagtatgtt cagacggcat gggattccgt ttgcgaatac gacggataaa
     5341 tcagtggaag agcttgccgt acacatcctt caggcgtgca agctcaaacg caggttttga
     5401 cgggttttga ttcggtttga aggtggaact gccgtctgaa atcaggtttc agacggcagt
     5461 tttatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt
     5521 acggagccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     5581 gaggcaacga cgtactggtt tagatttaat ccactatatg tttgtggggc ggatattttt
     5641 cagggctgta ttttgtccag acattcgagc agatcgagcg gcgtgcggat gtggaaatcc
     5701 gcctgccatg agccggtatc gtcttcggga gcgatgtagc cccattcggc gaggacggtt
     5761 ttcatgccgg cgttgcggcc tgcttggatg tcgcgttcgg catcgccgac gtagagtgtg
     5821 tgttgcgggt cggcgtggat ttgtccgcac gcatacagca tgggtttgat gctgggcttg
     5881 ggttcgccgc aggtgtcgcc gctgacgacg acggcgggtg ggatgatgaa gccgagtttg
     5941 ggggcgagtt tgtcggtgaa gcgcatgggt ttgttggtga tgatgcccca tttgatgccg
     6001 cgtctgtcga gttcggcgat gagttcgttt acgccgccga agagggtggt gtcttgggcg
     6061 tagcggctgt cgtattcgtc aaggtattcg gtgcgccatc gggcatagtc ggggtggtcg
     6121 ggggtgatgc ctgtgccgag cttgagcagt cctgctgcgc cgtggctggc ttggttgcgg
     6181 atttcgtcca tgcttttggg cggcagtccg tggcgggcga gcagggtatt gagtgcgccg
     6241 ccgaggtcta gggcggtgtc ggcaagcgtg ccgtcgaggt cgaagagtac ggcgtgtatc
     6301 atgtatgttc cttttcagac ggtatttttg ttctgtttca aactctgctc gaaatcttcc
     6361 agcatatcca attcgaagcg gctgaagccc gctttttcgc gcgcttcgat gttaacatag
     6421 ccgcggaaga taaacatatc gtaacgggca atcaggctgc ggaacagggc aatcggttcc
     6481 aaaccgcgtt cgcggcagag gtgttgatac caccggttgc cgatggcgac atggcccact
     6541 tcgtcgcggt aaataatgtc caacacgccg caggtttccg aatcgccgcg ctgcgccacc
     6601 ttcgcgcgta tccccggcgt aacgtctagc ccacgagctt ccaatacgcg cggcactaaa
     6661 gccatacgca acaaaggatc gtaggcggtt ttgtatgcca tatcccataa atgattgtgc
     6721 gcctcgaaat cgccgtaatc gaagccgaaa gcgcgcagcc tttcgcgcat caggcggaag
     6781 tggtacacct cttccttcgc cactttcacc cagtcgcgga caaactgaaa cggcagcgtg
     6841 cggaaacggt atgccgcgtc caaagccaga ttgacggcat tgaactcgat atgcgcaatc
     6901 gcgtgcagca tcgccgcata gccttcggtc gtgttcattt tgcgcggggt cagttgtgac
     6961 ggcgcgacca aaaccggctt gtccggccgc cctgcgcggg ggaaatccag cggcggcgtg
     7021 tttgtttcag caccgtccat gttctgaacg gcggcaaacg cctcatccgt cagccgtcct
     7081 ttttcatcgg ggtcgcccga aagcagggcg cgttccagca aagcataaat atcgggtttc
     7141 atctcaagtc cgccgtgttc ggaaaacaaa tattatagcg tttaaaaaaa acaagatgag
     7201 gcatataatc tccgcgattc ggcattccgc gcccaaaccg tcaa
//
LOCUS       DAWKQB010000107         7137 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.107, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000107 DAWKQB010000000
VERSION     DAWKQB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.107"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            176..2026
                     /locus_tag="WJL14_001597"
     CDS             176..2026
                     /locus_tag="WJL14_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ7656647.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPADKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENASDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAATSGLLDGNNAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNIIG
                     KEARKSPNAAALLSEMESGLSLEQSSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAVKRALVLFQNSQ
                     SAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAHEI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMANAAYYASLFGAPHIPLKQRMGIVPAR"
     gene            2315..2527
                     /locus_tag="WJL14_001598"
     CDS             2315..2527
                     /locus_tag="WJL14_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ7656648.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            2659..3105
                     /locus_tag="WJL14_001599"
     CDS             2659..3105
                     /locus_tag="WJL14_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ7656649.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(3430..3822)
                     /locus_tag="WJL14_001600"
     CDS             complement(3430..3822)
                     /locus_tag="WJL14_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ7656650.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTSPENTSAETAPRPAKKGLKLVK"
     gene            complement(3894..4499)
                     /locus_tag="WJL14_001601"
     CDS             complement(3894..4499)
                     /locus_tag="WJL14_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ7656651.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LGHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            complement(4601..6277)
                     /locus_tag="WJL14_001602"
     CDS             complement(4601..6277)
                     /locus_tag="WJL14_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ7656652.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPDE
                     PAVYTAVEALAGSPTPLVAHLFGQIDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHIQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNICGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADAPQGWQTVWQGARPRNKDSKFALIRKTGENILKTTD"
     gene            complement(6433..7062)
                     /locus_tag="WJL14_001603"
     CDS             complement(6433..7062)
                     /locus_tag="WJL14_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ7656653.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLVNLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
BASE COUNT         1866 a         1946 c         1903 g         1422 t
ORIGIN      
        1 gggcaaaggc agaggttcgc accaaaacgg caaatgattg aaaaatataa attatgtcgg
       61 aaacattcca aagccggtgc agtttcggca tataattcgg gcaaacgcct gttcagacgg
      121 cattttgtct tttccaaccc tgaccgttca gggttccgat ccttaaggaa atccgatgta
      181 cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg ccgcgtgttc
      241 ttcgacaaac acactgccag ccgacaagac cccggcagac aatatagaaa ctgccgacct
      301 ttcggcaagc gttcccaccc gccctgccga acccgaagga aaaacgttgg cagattacgg
      361 cggctacccg tccgcactgg atgcagtgaa acagaaaaac gatgccgccg tcgccgccta
      421 tttggaaaac gccagcgaca gtgcgatggc ggaaaatgtc cgcaacgagt ggctgaagtc
      481 tttgggcgca cgcaaacagt ggacgctgtt tgcacaggaa tacgccaaac tcgaacctga
      541 agggcgcgcc caagaagtcg aatgctacgc cgattcgagc cgcaacgact atacgcgtgc
      601 cgccgaactg gttaaaaata cgggcaaact gccttcgggc tgcaccaaac tgttggaaca
      661 ggcagccaca tccggcttgt tggacggcaa caatgcctgg aggcgcgtgc gcggactgct
      721 ggccggccgc caaaccacag acgcacgcaa ccttgccgcc gcattgggca gcccgtttga
      781 cggcggcaca caaggttcgc gcgaatatgc cctgttgaac atcatcggca aagaagcacg
      841 caaatcgccg aatgccgccg ccctgctgtc cgaaatggaa agcggtttaa gcctcgaaca
      901 gagcagtttc gcgtggggcg tattggggca ttatcagtcg caaagcctca atgtgcctgc
      961 cgccttggac tattacggca aggttgccga ccgccgccaa ctgaccgacg accaaatcga
     1021 gtggtacgcc cgcgccgcct tgcgcgcccg acgttgggac gagctggctt ccgtgatctc
     1081 gcatatgccc gaaaaactgc aaaaaagccc gacctggctc tactggctgg cacgcagtcg
     1141 cgccgcaacg ggcaacacgc aagaggcgga gaaactttac aaacaggcgg cagcaacggg
     1201 caggaatttt tatgcggtgc tggcggggga agagttgggg cgcaaaatcg atacgcgcaa
     1261 caatgttccc gatgccgacg caaacagcgt cctccgtatg gcggaagacg gtgcagtcaa
     1321 acgcgcactg gtactgttcc aaaacagcca atctgccggt gatgcaaaaa tgcgccgtca
     1381 ggctcaggcg gaatggcgtt ttgccacacg cggctttgac gaagacaagc tgctgaccgc
     1441 cgcgcaaacc gcgttcgacc acggttttta cgatatggcg gtcaacagcg cggaacgcac
     1501 cgaccgcaaa ctcaactaca ccttgcgcta tatttcgccg tttaaagaca cggtaatccg
     1561 ccacgcgcaa aatgttaatg tcgatccggc ttgggtttat gggctgattc gtcaggaaag
     1621 ccgcttcgtt ataggcgcgc aatcccgcgt aggcgcgcag gggctgatgc aggttatgcc
     1681 tgccaccgcg cacgaaatcg ccggcaaaat cggtatggat gccgcacaac tttacaccgc
     1741 cgacggcaat atccgtatgg ggacgtggta tatggcggac accaaacgcc gcctgcaaaa
     1801 caacgaagtc ctcgctaccg caggctataa cgccggtccc ggcagggcgc gccgatggca
     1861 ggcggacacg cccctcgaag gcgcggtata tgccgaaacc atcccgtttt ccgaaacgcg
     1921 cgactatgtc aaaaaagtga tggccaatgc cgcctactac gcctccctct tcggcgcgcc
     1981 gcacatcccg ctcaaacagc gtatgggcat tgtccccgcc cgctgacgta ccgatgccgt
     2041 ctgaaacccg cccggtcttt cagacggcat ttttatcccg aacggcattg acggcgaacc
     2101 ataaatataa gacaatccga aaattgtttt tcctgctttt tcaagcagct tgacacggca
     2161 caagccgacc cgttaggagg tgatgtttcc gtcacggcgc gtatcccgcc gccgcaaggc
     2221 acagcgatac ggtaaacctt caacaccgtc tgccctaccc tttccaccga tatgatgggc
     2281 agatgaaaca accgaattta ttaaaggaaa taaaatgcct gcaatccgcg taaaagagaa
     2341 tgaaccattt gaagtcgcta tgcgccgttt caaacgcgcc gtagaaaaaa ccggcctgct
     2401 gaccgagctg cgcgcccgcg aagcctacga aaaaccgact accgaacgca aacgcaaaaa
     2461 agcggcagcc gtaaaacgcc tgcaaaaacg cctgcgcagc caacaactgc cgcccaaaat
     2521 gtactaaacg ttcaagtaca gattacaggt cagccctgtg atatgaggac acaccgcaag
     2581 acctgctctg cggtgtgttt tgcttttcag acggcatcga aacccgccgt ttccatccga
     2641 catcccagcg aggacatcat gagcctgaaa atccgcctta ccgaagacat gaaaaccgcg
     2701 atgcgcgcca aagaccaagt ttccctcggc accatccgcc tcatcaacgc cgccgtcaaa
     2761 cagtttgaag tggacgaacg caccgaagcc gacgatgcca aaatcaccgc catcctgacc
     2821 aaaatggtca aacagcgaaa agacagcgcg aaaatctaca ctgaagccgg ccgtcaggat
     2881 ttggcagaca aagaaaacgc cgaaatcgag gtactgcacc gctaccttcc ccaaatgctc
     2941 tccgccgggg aaatccgtac tgcggtcgat gccgccgttg ccgaaaccgg cgcggcaggt
     3001 atggcggata tgggcaaagt gatgggcgta ttgaaaaccc gcctcgccgg caaagccgat
     3061 atgggcgaag tcaacaaaat cctgaaagcc gtgctgaccg cctgattgcc cgaatatcgg
     3121 acaaaatgcc gtctgaagcc cgtatcgcag gttcagacgg cattttcatg gtttgatgat
     3181 atttataaaa attttgcaac acgctcatgc acagcaactg ccgctgctcg ttggcgttgc
     3241 cgctctatcg cttggttagc tgcaccataa ttgcactcta atgctgatgc aataacgtcc
     3301 caatatcgaa ttggcagggg caacacggtt tcgatacacc gaaacgggtt ttgccgataa
     3361 acggattccg tttgcgcccc atcggacaaa atgccgtctg aaacacgatt ccgttcagac
     3421 ggcatagatt tatttgacca atttcaagcc ttttttggcg ggccggggcg cggtttcggc
     3481 agaggtgttt tcaggcgacg tgtcggggcg atacgcttcc aattcaaacc ccataccttc
     3541 gccggtctcc cgtgcgaaaa ggctgaggac gtgtccgaca ggtatccata tgtcgtgcgc
     3601 ctgtccgccg aagcgggcgg aaaagctgat ccaatcgttg tcgatttgaa ggttttgcgt
     3661 ggcggtcgcg ccgatgttga gcataatttc gttgtcgcgg acgtactgca tggggacgcg
     3721 cgtgtgttcg ttgacccaga caaggatgtg cggtgtgagg ctgttgtcgc tgcaccattc
     3781 gcaaagtgcg cggaggatgt agggtttggt tgaggtgggc atgatgggtt ccgtgtggtg
     3841 tgcctgaatg tgaaaatgcc tgcaaaacgg cgggttttgc aagcatttcg gacttatttg
     3901 cgcatggctt tttcggcggg tgtcagtgct tcgataaagg cttcgcgctg gaagatgcgc
     3961 tcggcgtatt tgagcagcgg cgcggcactt ttgcccagtt tgacatcgta gtggccgagc
     4021 cgccacagca gcggggcaag ggcgacatca atcatagaaa aatcttcgcc gaggatgtat
     4081 ttgcttttgc tgaacgaagg ggcaagcatg gtcagaccgt tgccgatggc ttcgcgcgct
     4141 tttgcctgtt ccttgttggt ggcggcgggg ttttccaaaa cttggacgtg gttaaacaat
     4201 tctttttcca tacggtacag caccagccgg cccctgccgc gcataacggg atcgccgggc
     4261 ataagctgcg gatgggggaa gcgttcgtcg atgtattcgt tgatgatgtt ggactcgtgc
     4321 agcaccaaat cgcgctcgac cagcacggga acttggttat acgggttcat gacggcgagg
     4381 tcttcgggct tgttgtaaat atcgacgtct ttgatttcaa aatccatacc tttttcgtac
     4441 aaaacgaagc ggcagcggtg gctgaagggg caggtaatgc cggaatagag ggtcatcata
     4501 atgattgtcg ctcctgtgtg atgcctgcaa aacggctgat ttataaaggt ttaatcgcgc
     4561 aattatacgc gatttccggc acttaatcca gaaattcggc tcaatctgtt gtttttaata
     4621 tattttcccc ggttttccgt atcagtgcga acttactgtc tttgttgcgc gggcgcgcac
     4681 cctgccagac cgtctgccag ccttgcggcg catccgcgtt ttggggcaag cggacgatgc
     4741 ggtagcggca ttgtacatcg ccgacgcggt gcggcaatgt gccgtactgc gtccaaacaa
     4801 tccgcgtgtg taggtcgccg ccgcctatgt cgatacactc gatgccgtct gaaagctccc
     4861 gttttaattc cggggaaagc gatgcctcca tactccggac gacgggcgcg tggcttttcg
     4921 ccgcgtccag ccacggcagg aacagcgtca tcagcaaagc ccaggtcagg gtaacgcctg
     4981 ccgcccagtt ggtaaccgcc tgcctgccgc atatgttttt gcgggtaatc gcccacagcc
     5041 acaagggtgt gaacagtacg gcaaccgcca tcggaatggg atcgatatca ggaacataat
     5101 acgggctgaa ataggcggcg cgttcggcaa gcttggcggg ccagccgtaa ttcatggcga
     5161 aaaagcccgt ccacaggaac acggcaaaca gtccgaacgc cataatgccg aaccagttga
     5221 caaacgccgc cgcgccgcgt ctcaggctgt ccagttgcgc cgcgccgaac agggcaagcg
     5281 gcggaagcag ccagacgagg ttatcctgaa aacgctgcgg attgacggca agcagcacca
     5341 aaacggcaag catccagacg acgcccaaaa tcccccagtc ggtcgaaaac aggcgcgtgc
     5401 ggcaaaccgt ccaaaccgcc agcggcagcg caggcaatgc aaaccaaagc aggtttttca
     5461 gatagtaaaa caaactgaat gccgtctgaa tgtgccgcac gccgccgaac gtaccgaaaa
     5521 cgtgatcgtc gagccattgc gcgaacagcg cgggctgcgt ttttgccaag agcagcgggt
     5581 aaacggtcat aagcggcagg gcaaaggcaa gcgaggcgac tgccgtcaac atcaaacgcc
     5641 tgctttgcca cggacggaaa aacatcagca cgggcagggg cagcatcagg gcaaatgccg
     5701 ccggataagc tgctgccaac gacatcagcg tccaacccgt accgagcaga aaagaggcgg
     5761 caatcacgcg ccggcgagcc aaagaataac cgtgcagcac cagtccggcg gcggcaaagg
     5821 cggcggcagc ggggttgaga aagtgtacgg tcggaatcag cccgatacag ccgatgagaa
     5881 tcaggactac gctgcgcccg tggtgtctgc ccaaaaagtt gaaaccggca aagccgcagg
     5941 aagtcagtcc gacaacggcg aaaaacacgc cggcaaagcg tgcggcatca tacgggtcgg
     6001 cagcccacgg cgacagcaaa tgtttgaacg cggcggcaac ccaaagatac acgggcggta
     6061 tgccgaaatc gatttgaccg aacagatggg caaccaaagg ggtggggctg cctgccagtg
     6121 cttcgacggc ggtatagacg gcaggttcgt caggattcca caaatcgtgg gaaaacacgc
     6181 cgggccacaa ccaggcaaac gccatcaaca gcaacagcca cggcttttcg tgggttttgg
     6241 cgggcgggcg ggcatcgggc ggggtatagg tcagcataag cgtgagaaga aatggacgga
     6301 ttgccaagtg tagcaaatat tcgcacaaag gtcgtgcaga gacggcatca gataaaatcc
     6361 gacagcatca gacacaaaaa gaccggcaac aaaaaagact gcacatggca gtctttgcag
     6421 atactatctt tttcataata ttttttcctg gcccgacaca gtccatagca tatcaaaact
     6481 gttgttttca atcaggatat atacccccaa tcctaaataa acaacagcaa caaaccatct
     6541 gctatatttt tccaaagttt ctccaacaga agggacttgt gctaattttt gggcagaaaa
     6601 aaccaagaga taaatcatga ctagaaaggt aagtaaagcc actatcaaat tcactaaatt
     6661 taaggtagta aaatatggaa caaagacacc aatattgtca gcaccacaac ttgcaaaagt
     6721 aatcatagcg actagaaaaa tcaggttttt attatctttg cgcaaaccct ctttggcaat
     6781 agcctctcca tcggaatctc ctaaaagcaa aactttgagg cctaggaaaa tcggaatcaa
     6841 gccgagcaaa cctaaaatct ctttactagg aatataatct aagacaaatg caaaaagcaa
     6901 acttagcaat atcaggctaa cagagcctag aaattgtcct aaatagatgt taatgatgtc
     6961 ttttctgctt tttcttttgg caaaaaataa cattaggata ataagtaagt ctacggctgt
     7021 cccagaatac aggattattg aagtaacgac attttgaatc ataaaacatc tcattcaaat
     7081 atatttttta atgtattcaa acattaaact ttgtagatgt caacttcaac cccatca
//
LOCUS       DAWKQB010000108         7102 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.108, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000108 DAWKQB010000000
VERSION     DAWKQB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.108"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(69..428)
                     /gene="rnpB"
                     /locus_tag="WJL14_001604"
     ncRNA           complement(69..428)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WJL14_001604"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJL14_001605"
     CDS             complement(507..1610)
                     /gene="prfB"
                     /locus_tag="WJL14_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ7656654.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIATGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1713..2606)
                     /locus_tag="WJL14_001606"
     CDS             complement(1713..2606)
                     /locus_tag="WJL14_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ7656655.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRRIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PSKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2948..3397
                     /locus_tag="WJL14_001607"
     CDS             2948..3397
                     /locus_tag="WJL14_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ7656656.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3471..3779
                     /locus_tag="WJL14_001608"
     CDS             3471..3779
                     /locus_tag="WJL14_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656657.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3895..4737
                     /locus_tag="WJL14_001609"
     CDS             3895..4737
                     /locus_tag="WJL14_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ7656658.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4727..5473
                     /locus_tag="WJL14_001610"
     CDS             4727..5473
                     /locus_tag="WJL14_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ7656659.1"
                     /translation="MQPEISAQYQHRFAQAIREGEAADGLPQERLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5495..6082
                     /locus_tag="WJL14_001611"
     CDS             5495..6082
                     /locus_tag="WJL14_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ7656660.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTALDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6088..6267
                     /locus_tag="WJL14_001612"
     CDS             6088..6267
                     /locus_tag="WJL14_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ7656661.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6508..6948
                     /locus_tag="WJL14_001613"
     CDS             6508..6948
                     /locus_tag="WJL14_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656662.1"
                     /translation="MIKETLMRPIFLSFVLFPILITACSTPDRSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1791 a         1891 c         1632 g         1788 t
ORIGIN      
        1 acacaaccta aataaaaatg ccgtctgaac catatttcag gtttcagacg gcatttgcct
       61 gtcggatgca caccggacag gcggtaagcc gggttctgtc tcggacagtc attcctctag
      121 gcataccgtt gccggtatgc tcaagcaacc tacccgaacg ctcggcgggc agcgtcattg
      181 cgttctgttt ggtcttgctc cgaatggggt ttggcctgcc gcatattgtt accaaatgcg
      241 cggtgcgccc ttaccgcacc ttttcaccct tgcctgtgct gccaaagcag ccatcggcgg
      301 ttttgctttc tgttccactt tccgtcgcgt taccgcgccc ggccgttaac cggcattcta
      361 ccctgcggag cccggacttt cctccccgta tgccttacgc gatacgcggc gactgtctgc
      421 ccgtcccgtg tgcggcgcgg attataacac gaaacgcaaa aatgccgtct gaaacggtac
      481 aggtttcaga cggcatacag cctaaactac acgccctgtt tcaggctggc ttcgataaag
      541 ccgtccaagt cgccgtccaa tacggctttg gtgttgccga cttcgtagcc tgtgcgcaag
      601 tctttaatgc gtgaggagtc caagacgtac gaacggattt ggctgcccca acctacatcg
      661 gatttacctt cttccaacgc ctgtttctct tcgttgcgtt tgcgcatttc caattcatac
      721 agtttggact tcaacatttc catcgcggcg gctttgttgg cgtgttgcga acggtcgttt
      781 tgacattgca ccacaatccc cgtcggctcg tgggtaatac gcacggcgga gtcggttttg
      841 ttgatgtgct gaccgcccgc gcccgatgcg cgataggtgt cgatacgcaa atcggcgggg
      901 ttgatttcga tttcaatgga atcatcgatt tcagggtaaa cgaacacgga ggcaaacgaa
      961 gtatggcgtt tgttgttcga gtcaaacggt gaatagcgca ccaagcggtg aacgccggtt
     1021 tcggtacgca gcaaaccata ggcgtattcg ccttccactc ggatagtcgc gcggttgatg
     1081 cccgcgattt cgccgtcgtc ttcttcgagg atttcgattt tgaagccttt gcgctcggcg
     1141 tagcggctgt acatacggaa cagcataccc gcccagtctt ccgcttccgt accgcccgcg
     1201 cctgcggtga tgtcgataaa gcagttgttc gggtcggcgg gctggttgaa catccgtttg
     1261 aactccaaat ccgccatctg tttttccagc cccgccacat cttcctgcac ggcggcaaaa
     1321 ccttcttcgt cgttttcttc gacggtcatt tcaatcagca tgcggttgtc ttcgatgccc
     1381 gtcgcgatgt tgtcgagcgt caacacgatg ccttcgagga ttttgcgctc tttgccgatt
     1441 tcttgggcgc gtttcgggtc gttccaaagt tcggggtctt cggaaagacc gataacttct
     1501 tccaatcggt ctttcttacc ctgataatcc atataaactc ggatgtcttc gctgcgcttt
     1561 tccaaatcat tcagggtatt gttgagctgg ttgattactt cggcttccat gattcttttg
     1621 ttctttcaaa attttagggg cgtattgtac gggattcggg tatttttttc tatggataaa
     1681 gccttctgga aacacgttcg gacggcatag cgtcaataac ggtatgccgc cagtttgcgt
     1741 ttgatttcag gcaatgcggc acgtgctgcc tcctcaccca accggatggc gcgttttttc
     1801 tgatcgaatc cgccgactgc acccaaatcc aaaacctgcg gtttgataac cacatccgcc
     1861 tgccccaact cattttgcaa cgcggaaacg ctcattacgt tcagcgtctg atcgagataa
     1921 gagaagaagc cttggctgat gtttttgctc ggacgggcgg aaatatcgac ggcaatcacg
     1981 aaattcgccc cctgccgccg ggcggcactg acgggcacgg gctgcgacag accgccgtca
     2041 acatatgtat gcctgccgat gataacgggt tggaacacat tgggaatggc ggcggaagcg
     2101 cgcacagcct gcccggcatt cccttgattg aaagcgacgg ccttgccggt ttcaaaatca
     2161 gtagcaacgg cggcaaattt gatgggaaac tgctgaatcc gcctgccgcc gacttttcgg
     2221 ttgatgtaat tttgcagctt ttcgcctttg ataaaaccac tggtggacaa ggttaaatcg
     2281 accaaatcgg ttttacctaa aatttcggct tccaattcga ggcggtcggg cgacataccc
     2341 gatgcaaaaa ggctgccgac tatcgaacct gccgatgtgc cggtaaccac cttcacagga
     2401 ataccgtttt ctttcaaaac cttaataata cctacatggg caaatccttt agatgcgccg
     2461 ccaccgagtg ccaaaccgac cactgcggcg ggtttggcgg tttgcaccgg cttgcgggca
     2521 gcattatttc ccgccgtgcc gcaggcggca agcaacgcgg cggcggcgat tgccaaaagc
     2581 ggtctgattt ttgaaaacgt taccatattt tccattcctt tatatatcgc accccgtcaa
     2641 aaagagggat tgcttttctt aacaaccccc tttgacagcc aagcaaatgg gggctttgtt
     2701 aagtcatcat caaaattaat atttcttttt ttcctttacg gaaattatat ttgaaggcat
     2761 actatccaag gtgggaatta tctcacaaca ccgccgttat ccaaatatcc cgcctttttc
     2821 ccttcctttc catcaaaata ctttcttttt atattcatta acttgttaaa tcattggctg
     2881 ccgggtgtca gtttttccga caaaatccgt ctaatggggt atcaacagaa ccaaaacagg
     2941 aacacttatg aaaatcggaa caacttggca gacggcatcc gctatgctgg ttttgcgtct
     3001 gtttgccgca tatgaatttt tggaatcggg tttgcaaaaa tggaacgggg agaattggtt
     3061 ttccgaaatc aacgatcagt ttccattccc gttcaacttg ctgccggacg cgttaaactg
     3121 gaatctcgcc atgtatgcgg agcttttact gcccgtactg ttgcttttgg gtttggcaac
     3181 gcgtctgtcg gcattagggc tgatggtcgt taccgccgtc gcttgggctg cggttcacgc
     3241 cggttcgggt tacaatgtct gcgacaacgg ttataaaatg gctttaattt atatcgtggt
     3301 attaatcccg ctgcttttcc agggtgcggg cggatggtcg ctggatacgc tgctgaaaaa
     3361 acggttttgc ccccaatgcc gtctgaagca agattgattc agtcgtggaa tctgacttta
     3421 aacattccaa ccttatctcg ttaacttgat attctgaaaa ggaaatagaa atgaacaaaa
     3481 acattgctgc cgcactcgcc ggtgctttat ccctgtcttt ggccgccggt gcagttgctg
     3541 ccaacaaacc ggcaagcaac gcaacaggcg ttcataaatc cgcccatggc tcttgcggcg
     3601 cgtccaaatc tgccgaaggt tcgtgcggcg cggctggttc taaagcaggc gaaggcaaat
     3661 gcggcgaggg caaatgcggt gcgaccgtaa aaaaaaccca caaacacacc aaagcatcta
     3721 aagccaaggc caaatctgcc gaaggcaaat gcggcgaagg caaatgcggt tctaaataat
     3781 cccacccctt caaaccaagc cgcgtttttc agtaaaatgc ggctttttta acggcaaaca
     3841 aagatttttt aacaagcaca tcattctttt gtgccatccg aaccgggtaa aaatatgatt
     3901 caacacgcag gcttgggcta ccgccgcgac ttggcggaag actttctctc gctttccgaa
     3961 aacagcccga tatgctttat cgaagccgca ccggaaaact ggctgaaaat gggcggctgg
     4021 gcacgcaaac agtttgaccg tgtggcggaa cggctgccgc tggcgttgca cggattgtct
     4081 atgtcgctgg gcgggcaagc accgctggat actgatttga tagacggcat caaagaaatg
     4141 atgcgccgtt acgattgcac gtttttctcc gaccatttga gctactgcca cgacggcggt
     4201 catctttacg atttgttgcc gctgcccttt accgaggaaa tggtgcatca tacggcgcgg
     4261 cgtatccgcg aagtgcaaga ccgtttgggc tgccgcatcg ccgtggaaaa cacgtcctac
     4321 tatctgcatt ccccgcttgc cgagatgaac gaggtcgagt tcctcaacgc ggttgcacgt
     4381 gaggccgatt gcggcattca tctggacgtg aacaatatct acgtcaacgc cgtcaatcac
     4441 ggcctgttgt cgccggaggc ttttttgaaa aacgttgacg cagatcgcgt gtgctatatc
     4501 catattgccg gacatgacgt ggaaacgccg gaattgttga ttgatacaca tggcgcggca
     4561 gttttgccga ctgtttggga cttgctcgaa cttgcctatg ccaagctgcc gacgattccg
     4621 cccaccctat tggaacgcga ttttaatttc ccgccttttg ccgaactcga agccgaggtc
     4681 gccaaaattg ccgactatca aacgcgtgcc ggaaaggaat accgccgtgc agcctgaaat
     4741 ctccgcccaa taccaacacc gtttcgccca agccatacgc gagggtgaag ccgccgacgg
     4801 actgccgcaa gagcgcctga acgtctatat ccgcctgata cgcaacaata tccacagctt
     4861 tatcgaccgc tgctataccg aaacgcggca atactttgac agcgaagaat ggagccgtct
     4921 gaaagaaggt ttcgtccgcg acgcgcacgc ccaaacgccc tattttcaag aaatccccgg
     4981 cgagttccta caatattgcc aaagcctgcc gcttttagac ggcattttgg cactgatgga
     5041 ttttgaatat acccaattgc tggcagaagt tgctcaaatt ccggatattc ccgacattca
     5101 ttattcaaat gacagcaaat acacaccctc ccctgcggcc tttatccggc aatatcgata
     5161 tgatgttacc gatgatttgc atgaagcgga aacagccttg ttaatatggc gaaacgccga
     5221 agatgatgtg atgtaccaaa cattggacgg cttcgatatg atgctgctgg agataatggg
     5281 ttcctccgcg ctttcgtttg acaccctcgc ccaaaccctt gtcgaattta tgcctgagga
     5341 cgataattgg aaaaatattt tgcttgggaa atggtcaggc tggactgaac aaaggattat
     5401 catcccctcc ttgtccgcca tatccgaaaa tatggaaggc aattccccgg gccaaaacca
     5461 tctatccgca taaaattacc ttgttcccga tactatgccg ctacccgacc tgaccgatgc
     5521 cgaattaata gagtcgcgta aactgcttct gcattttgcg cggcttcagt tgcccgacca
     5581 ccctgatttg gctgaagatt tagtgcagga aacattgctg tccgcataca gcgcaggcga
     5641 cagttttcaa ggcagggcac ttgtcaacag ctggcttttt gccatattga aaaacaaaat
     5701 tattgacgca ttacgtcaaa tcggaaggca gaggaaagtc tttaccgcac tggatgacga
     5761 gctactggat gaagcatttg aaagccattt ttcccaaaac gggcattgga cgccggaagg
     5821 gcagccgcaa cattggaaca ctccggaaaa atcattaaac aacaacgaat tccaaaaaat
     5881 tctgcaaagc tgcctataca acctgcctga aaacaccgca cgggtattta ccctgaagga
     5941 aatactcggt ttttcatccg acgaaataca acaaatgtgc ggtatcagca cgtccaacta
     6001 ccacaccatt atgcaccgcg cccgagaatc attgcgccaa tgcctgcaaa tcaaatggtt
     6061 caaccaagaa aacccgaagt aaacgttatg aaaaaatgcc gcgatatcgc cctgcttctt
     6121 tccaaacatc aggaccggga aaccaccccg ggcgagaaga tttccatata cacacacctg
     6181 ctgttctgtc cgtattgccg tgaatataaa agacaacttc aaaccatcaa aagatcactg
     6241 gcaaaaacaa ccagaacttc aaaataaatg ccgtctgaaa aggcttcaga cggcataagc
     6301 tgacggaaac aaatcaaacc gatttactgt tatcggcagt tcatccataa tacacgcttc
     6361 aaaagcagca tattcctcca tacggaatat ataaatacgc aaaacacgaa ggctgcatca
     6421 atttgctata tttgctttat ttgccttatt tcacagacgg cgctacccct cccgcccaac
     6481 ccgttctttc tgaatgagca gatttcaatg attaaggaaa ccctaatgcg cccaatcttc
     6541 ctatctttcg ttttattccc tattttgata accgcctgca gcacaccgga caggtctgcc
     6601 cgatgggaaa atatcggcac aatctcaaac ggcaatattc atacatatat caataaagac
     6661 agcgtgagaa aaaacggaaa tctgatgatt ttccaagata aaaaagttgt taccaatcta
     6721 aaacaagaac gttttgccaa cacccccgca tacaagactg ccattgccga gtgggaaatc
     6781 cactgcaaca acaaaacata ccgcttaagt tcgctacaat tgtttgatac aaaaaacacg
     6841 gaaatttcca cacaaaacta cacagcctct tccctccgcc cgatgagcat cctgtccggg
     6901 acattaaccg aaaaacaata tgaaaccgta tgcggaaaaa aactctgatt gcaacttata
     6961 cacaaactta cccacaaacc ttatcataaa aatgccgtct gaaatactga aatatcagca
     7021 tttcggacgg cattttgcca tttctccgaa aagttatcct cgaagttatc cacattattt
     7081 ttttaaaccg gcttccatcc ga
//
LOCUS       DAWKQB010000109         6836 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.109, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000109 DAWKQB010000000
VERSION     DAWKQB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.109"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..4088)
                     /gene="hrpA"
                     /locus_tag="WJL14_001614"
     CDS             complement(<1..4088)
                     /gene="hrpA"
                     /locus_tag="WJL14_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HEZ7656663.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDNKGAVLNNDRNNNPFLVKGSAQLILNEVRGTASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARVDAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGG"
     gene            complement(4198..5940)
                     /gene="hrpB"
                     /locus_tag="WJL14_001615"
     CDS             complement(4198..5940)
                     /gene="hrpB"
                     /locus_tag="WJL14_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ7656664.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLVHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            complement(6001..6240)
                     /locus_tag="WJL14_001616"
     CDS             complement(6001..6240)
                     /locus_tag="WJL14_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7656665.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTDL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
BASE COUNT         1565 a         1581 c         1680 g         2010 t
ORIGIN      
        1 tagccgccgg gtgcggacaa tttgggcgga gtagggcttt cgaagctggg cagttttagc
       61 gtttcgatag tgctgccgcg tccggcctgt ttctgccata cggttgagtt ggtttctaat
      121 ttttcttccg actggatacg gttcacaatg cctttgagga taattttcgc atcgacacgg
      181 gctttttcgc ctacacctgc ctgaatgtcg gcaccggcca gcgtggtttt gaattcggta
      241 ccttcgagca cggtatccca gcctgaacgg gtggctgcag tttgggcgac gacgcggaca
      301 ggcaatttgg tttcgttcag ttcgttttta ctgtaattgc tcttacctac cttgatgccg
      361 ataaagcggc ggcttttttg gacatccaac tcgtgcttgt ggatgccttc ttctgccagc
      421 agttgcagct cttcacccgc aaccagggta actttacctg caggggcatt gaagcgggtg
      481 gtattagctt cgatgttgcc gcctgcctga agcgtgatac cgttggcggt cagctcgacg
      541 ggggctggca taatcaggtg gtcgcgggtg ctggtaaact tggtttttct gatgattttg
      601 ccgcttttac ctttggtttt taagaaggta taggcatcgt tttgtccagc ctccagtaca
      661 atatcactat gggctttgat gtctatgctg cctgagggag ctttgatttc ggatgcaccg
      721 ataataatac gtgcatcatc gagtgccgca gctgcatgaa tacttacccc cgtacgtccg
      781 gtcaaacgtg aaggcttgtt cagagcagct ttgtcgtagt gactcttgta ggtgggcttg
      841 ccaatttcat attggtcggt tatgccgtca atcagaatag cagccgcctc tgaatctgct
      901 gcctttggca atacgcctgc ggcgtgaagg ttcagttttt tggaagcggt aatatcggaa
      961 ccgctgattt cgatgccttg tgcggaaatc aagtcaatat tttgtgcaga aagcttggct
     1021 tgcaggtatt ctttgccttt gggtttttta cctttaactt ccttgttgat ggcttgaata
     1081 tagaaagcga gacggtcgcg ttcttcttgc agggttggaa tcagcttgct tttaggcgag
     1141 ctttttttca actgcgcaat ctgctgttcc aattctttgg atttttggtt gagttcagcc
     1201 gctttttgtg taggaaaata attgctgaat gagttgttta cggcttcgat attcaacttg
     1261 cctttggtgg tggcgacaac caagttttta ccggctgtaa ttttagaacc tcttaaatct
     1321 gtttctcctg taaccagacg gatattgcct tttgcttcca atgaggaaac ttgagcacta
     1381 ggcgcacctg catttcctcc ttttgcagac aacagcaatt ttccgcctgt tttgatgctc
     1441 aggtcggtat gcgcactgat gcggttggca ggttcgatgg ttaatgtgcc gctacctgct
     1501 tcaatattca gccgtccggc caatggtttt aattcggcat tatcttccaa agtcttggtc
     1561 gaaacggtac tccaattgat gttgccgcgc ttgactaggg cggtaccggc agtaaggttg
     1621 attgcacccg ctctcagcgt ggtgttgtcg gcaatttggg aatagcgcgc attgagtgcc
     1681 aatacaccgt tagccaccag cttgttggca gaaggcagtt tgtcgttttg ccaaatctgg
     1741 ctgccggtaa tgcttagatg acggtgtgcg taggcatcta cttggttgag cgttacccgc
     1801 tcgtgttgtg cattaagatg cgtattatgg gtagactcca gcttggtatt ttctatgctc
     1861 aatgcccggt cggaatgaat gttcaatgcc ccgcttttgg catggacgtt aaggtttttt
     1921 aagtcggcat taccaccgtt gtttttgatg ctgatgtgtt ttccgttgat tgaattgcgt
     1981 tgttttccgt caccaagctg aataccgttg ccggcaacca acgtaatatc tcctgaagat
     2041 gaagtgatat tggtattgtc tgctttcaga cggcctttac caaccgatcc tgcattgaca
     2101 tcggccttgg ctgtcagggt attgtgaccg gtaaagtcgg cattaccgtt ggccaataag
     2161 gatacatgac cgtctgcaga aacagcatga aggccgtctg aaacgatatt gccctgcaat
     2221 gcggtggttt cgagagcctt ggctgcgttc agcttggtat tgcgaagctg aatattgcct
     2281 ttggctgcct gaatgtgcag attacccgag ttggtacgca gattggtatt ggtaacattc
     2341 agcgagcctg cctccacacc catgtctttt gaggcagtga gggttttact ggtgccggta
     2401 atatgggcag cgttatccga tttcaaatgg atgctggcgg cagacaaatc tttatcaaca
     2461 ttcaaattca gatctttacc tgtatgaaca tacagattgc cgggagtatt cagattggta
     2521 gttttggcag taatgttatc gtctgccagt aaagcaagct gcttgccgcc cttgatactg
     2581 cctccgttta agcggatatc ggaggtaact gttgaagctt ccaaagaaag cggtttgcct
     2641 gcttcgatgt gtgcggtgtc tttggcatct attacggcgg aactgctgat ggtgccgttg
     2701 gataatacgg taacatctgc cccggtaatg cgtgtgttat tgcctaattc ggcgttgcct
     2761 ttgctggaac tgtatacggt agtgccagtc tgaatactgg cctccttgat gacggtacgg
     2821 ccgccggtcg acagattggt cgaaccttta gcattgttca catttgcttt gctttcgata
     2881 accaaattac ggccggcatt taataccgta gcagcagggc ggttgccgtt gttttgaacg
     2941 accgctccgt tacgcaaacc gatgtcctcc ccggtatcaa taaccaataa gcctttgctc
     3001 tctatccggc cgccattgga gataaatgcg cctgctgcgc ctgtttcggt ggtttcgatg
     3061 gagagataag tcggtgatgc ttcagtgctg tcggcagtgg tggcgatgcg gccgctgttt
     3121 tcaatacggc cggaggaagt aacgatcaat tgcttggccg cttcgagtgt gcccgcattt
     3181 ttgacaccca cgcctttttc attggcaatc agggtaatgc tgtcggcgta cataccaccc
     3241 aatgcggcag tatcgagggc aacggtcggt ttcgtacctg ctgccgttcc tgcgctgatt
     3301 tcaccgctgg cgtaatctac tttctgtgcg ccggtcgaaa ccgccaggtt tttaccctgc
     3361 aatttcccct gcaaagcaac cgcacgggca agcacttcgg tgtaatcggc tccgcctttg
     3421 tcgttccaac ctgacgtacc gacggtcaat gtgccttggc gcacgtcaaa tcccgtcaag
     3481 gcaccgtctt tgccgatttg aggcgtaccg gtggttaaga taccgcggcc gacgttttta
     3541 aagccgccgc cattaacggt aatgccgttg gggttggcaa taatcacgtc ggccttttga
     3601 ccgcctacgg taacgatgcc gttgagtttg ctagccgtac cgcgtacctc gttcaaaatc
     3661 aattgcgcac tgcctttgac cagaaacgga ttattgttac ggtcgttgtt taacactgcc
     3721 cctttgttgt caacatcaaa ctgcgtatag cggttgtggc tcaatccgcg tccattcgga
     3781 gtttggatat tcaccaaggg ggcaccagtg ttggttttaa ggataacgac ctgctggttt
     3841 ttaggtgctg atttgtcggt ggtaatttgg gcatgggcag gcaataccat actcagggaa
     3901 accaaagagc agaccaaggt tttaagggtg gttttgagtt tgccgcaaag gtcgcctgaa
     3961 gttttcagtg aaacagaaac cgaactgcct gcctgtttac ctttgccctg gctgttggca
     4021 gtttcggcta ctgcaaccat ggtgctgtgc tttttactaa agataatgcg atgtaaacct
     4081 ttattcatgt ctattccatt ttgaagatga acgtactgcg cgccaagtac gtaggtaaag
     4141 tttgacggtc tgaggataag gaaagaccgt ctaaatatca gtaaaaaaat tcagaggtta
     4201 gaaactgtaa ttcaagttga agccgtaaac ggtgttggcc gtctgaaaac cttcgggctt
     4261 gtgcagcggc ttgccggcaa ataagtcata agcaaatata ccgcctactt tatggctgcc
     4321 tctgaagccg gccactgcac ccgccagccg cttgcccgag gcatattggg cactttcgcc
     4381 cgatacccga ccatagtccg cgccgagata gaattgatgg cgcggatgga aatgccagcc
     4441 caaagtgttc tgccagtaga aaccacgctc tccggaaagg ctctgttcac cgtcaaatcc
     4501 tcgaacggtg tagcggctgc cgatagacaa cttgtcttgg gcaaccaaag gcgttttgtt
     4561 ccattgtgca tggatagcgg tttcgtagga aaactgctgt ttgcccaaca taaacggggc
     4621 cgctgcatcc aatccggcgg ttatgatttt catacgggat gtgcctggaa tagtaccgcc
     4681 gccgttttct tccggtgcgg gcatactttg gcgcataccg gtaccccgtt tgtaagataa
     4741 cttgccgtcc agttgccagc ggttgaggta agcacggtgg cgcaattcgg cttcccagcc
     4801 tgcagagcgg cggcgttgca cttcgatttc ggcatcgtcg atgtatttat aggtttggcg
     4861 tgtccataat ttcattccga ctgaagtttt atgaaacctg ttacgccaaa gcatgcgctc
     4921 ggcggccagg ctgctctgat attgtttgcc gttgtaatcg taattgacgg aatagccttc
     4981 ggttgcttcg tggtaacgat gtccattgtg attaaaagaa aacagccatt tttttacggg
     5041 caccgaataa tgcacgctgt aacttctgga tccgctttca gtttccgtac cggtggcatc
     5101 agtcaagtcc gttttgtgca ccaaaccgcg tccatatgaa acataaaaca aatcgcttaa
     5161 gcccaaaggg ttatcgaacg ataaagcgac atttccttga tatttgccgg tcgttttgcc
     5221 gcccgcatca tctataccga tactgaaccg tatgggttta ttctgctgcc atttgatctg
     5281 taaatcgctt ttgccttctt cttcggacgg tataatctga atatctgttt taacactcga
     5341 caaacgacgc aggttttcca agccctgctc tacatcgcga agattgagaa ttttgttcct
     5401 atataaggga aatttgttat tgaatgtact aatactgcct tcggcagact tcccatcccg
     5461 tttttcttca tagcggatat cccctatttc gcctgctgat acccgtaatt tcagaattcc
     5521 cgaatccata ttctgtggtt ggataatagc ttgggaagtg aggtagccac gcacgatcag
     5581 tatctgttgc gcagcttttt gtagcctgct caaattattg gaacctaaac acatcccagt
     5641 tttaaaagct gtttctttca tgagcacaga aggaagaaaa gaaaatttgc gcgccgtctt
     5701 atcatctaaa ctaatgtaat ttacccgagt acacggtgtt tcatcttcac tcaggacata
     5761 attgttcttc tccaatggtt gctcgaaacg gacatttgca tcagttaaca attcagcatc
     5821 tatgtgctgc tgacgctgca tggaacggat aagttctgca tcgttttcat cggcagctaa
     5881 ggttttaagg ggtatgacag ccaggataac caacagacat ggagcaggaa aaaatttcat
     5941 gacatcaata ttattttagc aatatttact attttgtcat aaatttaaaa gtatttacag
     6001 ttatagaatg agacctttgc aaaattcccc aaaattccca ccaagacatt taggggattt
     6061 tggggaattt tgcaaaggtc tcggacagta ttttgaacgc agtgcgcgta aatccgtatg
     6121 gaaaccatga aatcccgcca cagccgccag acatgccaag ccgcattctg atatttctgt
     6181 ttgcaggata acaggcagct ttttctttaa gcccaaagac aggttttgca gatggggcat
     6241 agatttcctt tttgaaaaat agggattagg aagttggatg tattttagaa aggccgcctg
     6301 aaaaggtttc agacgacctt ttgcgactag ctgctatttt atttaaagct tttctctaac
     6361 aaacgagcta atatttttcc tgtaataaaa cagataaaaa acagcatcca atacgtcaga
     6421 ttggaaaaat cggtcgtata gagaatcaac atataaagaa gcagcatgat gccgagtgcg
     6481 atgaattgat aatgtttggc aaacatcatg acctcctcaa ctattaaggc aaaccgcctg
     6541 aatattctcg ttcaatcgtt tcggcaattt ccctataacg tcgataccat gaccagtcga
     6601 aattttcaat ggcatggctc gcaaacgtac caaattcagg catccctatg cggctacctg
     6661 ctaaagctcc gattgtagct ccccaaccag gcggattact gaacgtattg tttggcaatc
     6721 ctaaagattt atcaggattt cccgtatcgt taagccctgc agattcgcaa atttcgcaat
     6781 aatatgagct ttgttacgca ttacctgagc ctccgaccca agtcatttca tgaaca
//
LOCUS       DAWKQB010000110         6782 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.110, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000110 DAWKQB010000000
VERSION     DAWKQB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.110"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJL14_001617"
     CDS             complement(75..1421)
                     /gene="mnmE"
                     /locus_tag="WJL14_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ7656666.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAQALCGKTPKP
                     RVATYADFTDEDGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPLELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1552..1707
                     /locus_tag="WJL14_001618"
     CDS             1552..1707
                     /locus_tag="WJL14_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656667.1"
                     /translation="MEKRNTYALLLGISSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1704..2135
                     /locus_tag="WJL14_001619"
     CDS             1704..2135
                     /locus_tag="WJL14_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ7656668.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVFALACAFVIGRSRDQGKN"
     gene            2269..6618
                     /gene="app"
                     /locus_tag="WJL14_001620"
     CDS             2269..6618
                     /gene="app"
                     /locus_tag="WJL14_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ7656669.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGRNPDQHRFSYQIVKRNNYKPDNSHPYNGDYHM
                     PRLHKFVTDAEPVEMTSDMRGNTYSDKEKYPERVRIGSGHHYWRYDDDKHGDLSYSGA
                     WLIGGNTHMQGWGNNGVVSLSGDVRHANDYGPMPIAGAAGDSGSPMFIYDKTNNKWLL
                     NGVLQTGYPYSGRENGFQLIRKDWFYDDIYRGDTHTVFFEPRSNGHFSFTSNNNGTGT
                     VTETNEKVSNPKLKVQTVRLFDESLNETDKEPVYAAGGVNQYRPRLNNGENLSFIDYG
                     NGKLILSNNINQGAGGLYFEGDFTVSPENNETWQGAGVHISEDSTVTWKVNGVANDRL
                     SKIGKGTLHVQAKGENQGSISVGDGTVILDQQADDKGKKQAFSEIGLVSGRGTVQLNA
                     DNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNATTTSTVTITGNESI
                     TQPSGKNINRLNYSKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLSGGTNLNG
                     NITQTNGKLFFSGRPTPHAYNHLGSGWSKMEGIPQGEIVWDNDWINRTFKAENFHIQG
                     GQAVISRNVAKVEGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEKTITDDKV
                     IASLTKTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNGNLSLVGN
                     AQATFNQATLNGNTSASGNASFNLSNNAAQNGSLTLSDNAKANVSHSALNGNVSLADK
                     AVFHFENSRFTGQLSGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNSAYRHDAA
                     GAQTGSVSDTPRRRSRRSLLSVTPPTSVESRFNTLTVNGKLNGQGTFRFMSELFGYRS
                     DKLKLAESSEGTYTLAVNNTGNEPVSLDQLTVVEGKDNKPLSENLNFTLQNEHVDAGA
                     WRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAGRDAAEKT
                     ESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETRPATTAFPRARRARR
                     DLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDRRNAVW
                     TSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDDGIGNS
                     ARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGGKIRRRVLHYGIQARYRAGFG
                     GFGIEPYIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHISITPY
                     LSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAKGPQLE
                     AQHSAGIKLGYRW"
BASE COUNT         1789 a         1976 c         1675 g         1342 t
ORIGIN      
        1 aaacggatcg gattcccgtt tttatgggat aacggaatgt tcagccggac accgccacga
       61 tgctttcgat ccgtttattt tccgatgcag aatctcgaaa aaatcacgcc gagcaggtcg
      121 tccgccgtaa actcgcccgt gatttcgccg cacgcgacct gcgccagccg caagtgttcg
      181 gcaaacagct cgatttgatg gttgccgcac aatgccgcca gcgacaattc ttcctgcgct
      241 gctttgagtg cgttgacgtg ccgcgtccgc gccaaaaaca gcccttcgct ttcgccctgc
      301 caaccggcct cgcgcaacaa cgtccgtttc agcgcgtcca agccgtcgcc ggttttcgcc
      361 gacagtgcga tgacggtttc cgcgcccgta ccgaacccgc ctgccgcgtg tgcgtgcaaa
      421 tcggatttgc tgtggatttc gatgcgtttc aactccagcg gcaacgcgtc caaaatcgcc
      481 cgtgtctttt cattcaaacc ctcgcgcgga tcgaccaaca ccagcgcgac atcggcttcg
      541 gatacggctt tgcggctgcg ttcgatgccg atacgctcga ccacgtcgtc cgtctcgcgc
      601 aaacctgccg tatcgacaat atgcaccggc acgccgtcaa tcaggatacg ttccctgacc
      661 gcgtcgcgcg tcgttccggc aatatcggta acaatcgcca cttcgtcgcc cgccaacgcg
      721 ttcagcaggc tggacttgcc cacattcggc gcgccgacca atacgacatt cagaccttcg
      781 cgcaaaatcg cgccctgctg cgcgttggca agcacatcat ccacggcgcg gcgcaagccg
      841 tccagtttgc cgcgtgcgtc tgccgcttcg agaaaatcaa tgtcttcctc gggaaaatcc
      901 agcgtcgctt cgaccagcat ccgcaaggta atcaaatctt cgaccagacc gtgtatccgc
      961 cgcgaaaaat cgcccttgag cgagcgcaac gccagacgcg ccgccgaacg gctggatgcg
     1021 tcaatcaaat ccgccacgcc ttccgcctgt gccaagtcca gtttgtcgtt caaaaacgca
     1081 cgtttggtaa actcgcccgg ttcggcaagg cgcgcgccca attccaaaca gcggttcagc
     1141 agcatatcca tcaccaccgg cccgccgtgt ccctgaagct cgatgacatc ttcgcccgta
     1201 aaacttgccg gtgcggcaaa aaacagtagc agcccgctgt cgattgcctg accgtcctcg
     1261 tccgtaaaat cagcataggt tgccacgcgc ggcttgggcg ttttaccgca caaagcctgc
     1321 gccatcggca gcaggttttt ccccgatatg cgtatcacgc ccacgccgcc gcgccctggt
     1381 gcggtagcga ctgccgcaat cgttggaacg ttatccgaca taaaaccccc gaaaattcaa
     1441 aacagccgcg attatagcaa atgccgtctg aagtccgacg gtttggcttt cagacggtat
     1501 aaaaccgcaa aaatgcttga taaatccgtc cgcctgacct aatataacca tatggaaaaa
     1561 cgaaacacat acgccctcct gctcggtata agctcgctgc tgggtctgtt ccatcccgca
     1621 aaaaccgcca tccgccccaa tcccgccgac gatctcaaaa acatcggcgg cgattttcaa
     1681 cgcgccatag agaaagcgcg aaaatgaccg aaaacgcaca ggacaaggcg cggcaggctg
     1741 tcgaaaccgt cgtcaaatcc ccggagcttg tcgagcaaat cctgtccgac gagtacgtgc
     1801 aaataatgat agcccggcgt ttccattcgg gaccgttgcc gccgccgtcc gacttggcgc
     1861 aatacaacga cattatcagc aacggggcag accgcattat ggcaatggcg gaaaaagaac
     1921 aagccgtccg gcacgaaacc atacggcaag accaaacctt caacaggcgc gggcaactgt
     1981 acggcttcat cagcgtcatc ctgatactgc tttttgccgt cttcctcgta tggagcggct
     2041 accccgcaac cgccgcctcc cttgccggtg gcacagtgtt tgccttggcg tgtgctttcg
     2101 tgattggaag aagccgagac caaggcaaaa attaatgaca aatcctaggg cgtgcttcat
     2161 atccgcccga acgccgaacc gcacatatag gcacatcccg cgcgccgcct ggaagcggaa
     2221 gccgcgccct cccaaacaaa cccgaatccc gtcagataag gaaaaataat gaaaacaacc
     2281 gacaaacgga caaccgaaac acaccgcaaa gccccgaaaa ccggccgcat ccgcttctcg
     2341 cctgcttact tagccatatg cctgtcgttc ggcattcttc cccaagcttg ggcgggacac
     2401 acttatttcg gcatcaacta ccaatactat cgcgactttg ccgaaaataa aggcaagttt
     2461 gcagtcgggg cgaaagatat tgaggtttac aacaaaaaag gggagttggt cggcaaatca
     2521 atgacaaaag ccccgatgat tgatttttct gtggtgtcgc gtaacggcgt ggcggcattg
     2581 gtgggcgatc aatatattgt gagcgtggca cataacggcg gctataacaa cgttgatttt
     2641 ggtgcggaag gaagaaatcc cgatcagcac cgtttttctt accaaattgt gaaaagaaat
     2701 aattataagc ctgacaattc acacccttac aacggcgatt accatatgcc gcgtttgcat
     2761 aaatttgtca cagatgcaga acctgtcgaa atgacgagtg acatgagggg gaatacctat
     2821 tccgataaag aaaaatatcc cgagcgtgtc cgcatcggct caggacacca ctattggcgt
     2881 tatgatgatg acaaacacgg cgatttatcc tactccggcg catggttaat tggcggcaat
     2941 acacatatgc agggttgggg aaataatggc gtagttagtt tgagcggcga tgtgcgccat
     3001 gccaacgact atggccctat gccgattgca ggtgcggcag gcgacagcgg ttcgccaatg
     3061 tttatttatg acaaaacaaa caataaatgg ctgctcaacg gagttttaca aaccggctac
     3121 ccttattccg gcagggaaaa cggtttccag ctgatacgca aagattggtt ctacgatgac
     3181 atttacagag gcgatacaca taccgtcttt tttgaaccgc gcagtaacgg acatttttcc
     3241 tttacatcca acaacaacgg tacgggtacg gtaacagaaa ccaacgaaaa ggtttccaat
     3301 ccaaagctta aagtacagac agtccgactg tttgacgaat ctttgaatga aactgataaa
     3361 gaaccagttt acgcggcagg gggtgttaat cagtaccgtc caaggttaaa caacggtgaa
     3421 aacctttctt ttatcgatta cggcaacggc aaactcatct tatcaaacaa catcaaccaa
     3481 ggcgcgggcg gtttgtattt tgaaggtgat tttacggtct cgcctgaaaa caacgaaacg
     3541 tggcaaggcg cgggcgttca tatcagtgaa gacagtaccg ttacttggaa agtaaacggc
     3601 gtggcaaacg accgcctgtc caaaatcggc aaaggcacgc tgcacgttca agccaaaggg
     3661 gaaaaccaag gctcgatcag cgtgggcgac ggtacagtca ttttggatca gcaggcagac
     3721 gataaaggca aaaaacaagc ctttagtgaa atcggcttgg tcagcggcag gggtacggtg
     3781 caactgaatg ccgataatca gttcaacccc gacaaactct atttcggctt tcgcggcgga
     3841 cgtttggatt taaacgggca ttcgctttcg ttccaccgta ttcaaaatac cgatgaaggg
     3901 gcgatgattg tcaatcataa tgccacaaca acatccaccg ttaccattac agggaatgaa
     3961 agtattacac aaccgagtgg taagaatatc aatagactta attacagcaa agaaattgcc
     4021 tacaacggtt ggtttggcga gaaagatacg accaaaacga acgggcggct caaccttgtt
     4081 taccagcccg ccgcagaaga ccgcaccctg ctgctttccg gcggaacaaa tttaaacggc
     4141 aacatcacgc aaacaaacgg caaactgttt ttcagcggca gaccgacacc gcacgcctac
     4201 aatcatttag gaagcgggtg gtcaaaaatg gaaggtatcc cacaaggaga aatcgtgtgg
     4261 gacaacgact ggatcaaccg cacgtttaaa gcggaaaatt tccatattca gggcgggcag
     4321 gcggtgattt cccgcaatgt tgccaaagtg gaaggcgatt ggcatttgag caatcacgcc
     4381 caagcagttt ttggtgtcgc accgcatcaa agccatacaa tctgtacacg ttcggactgg
     4441 acgggtctga caaattgtgt cgaaaaaacc attaccgacg ataaagtgat tgcttcattg
     4501 actaagaccg acatcagcgg caatgtcagc cttgccgatc acgctcattt aaatctcaca
     4561 gggcttgcca cactcaacgg caatcttagt gcaaatggcg atacacgtta tacagtcagc
     4621 cacaacgcca cccaaaacgg caaccttagc ctcgtgggca atgcccaagc aacatttaat
     4681 caagccacat taaacggcaa cacatcggct tcgggcaatg cttcatttaa tctaagcaac
     4741 aacgccgcac aaaacggcag tctgacgctt tccgacaacg ctaaggcaaa cgtaagccat
     4801 tccgcactca acggcaatgt ctccctagcc gataaggcag tattccattt tgaaaacagc
     4861 cgctttaccg gacaactcag cggcagcaag gatacagcat tacacttaaa agacagcgaa
     4921 tggacgctgc cgtcaggcac ggaattaggc aatttaaacc ttgacaacgc caccattaca
     4981 ctcaattccg cctatcgcca cgatgctgca ggcgcgcaaa ccggcagtgt gtcagacacg
     5041 ccgcgccgcc gttcgcgccg ttccctatta tccgttacac cgccaacttc ggtagaatcc
     5101 cgtttcaaca cgctgacggt aaacggcaaa ttgaacggtc aaggaacatt ccgctttatg
     5161 tcggaactct tcggctaccg aagcgacaaa ttgaagctgg cggaaagttc cgaaggcact
     5221 tacaccttgg cggtcaacaa taccggcaac gaacccgtaa gcctcgatca attgacggta
     5281 gtggaaggga aagacaacaa accgctgtcc gaaaacctta atttcaccct gcaaaacgaa
     5341 cacgtcgatg ccggcgcgtg gcgttaccaa ctcatccgca aagacggcga gttccgcctg
     5401 cataatccgg tcaaagaaca agagctttcc gacaaactcg gcaaggcaga agccaaaaaa
     5461 caggcggaaa aagacaacgc gcaaagcctt gacgcgctga ttgcggccgg gcgcgatgcc
     5521 gccgaaaaga cagaaagcgt tgccgaaccg gcccggcagg caggcgggga aaatgtcggc
     5581 attatgcagg cggaggaaga gaaaaaacgg gtgcaggcgg ataaagacag cgccttggcg
     5641 aaacagcgcg aagcggaaac ccggccggct accaccgcct tcccccgcgc ccgccgcgcc
     5701 cgccgggatt tgccgcaacc gcagccccaa ccgcaacctc aaccccaacc gcagcgcgac
     5761 ctgatcagcc gttatgccaa tagcggtttg agtgaatttt ccgccacgct caacagcgtt
     5821 ttcgccgtac aggacgaatt ggaccgcgtg tttgccgaag accgccgcaa cgccgtttgg
     5881 acaagcggca tccgggacac caaacactac cgttcgcaag atttccgcgc ctaccgccaa
     5941 caaaccgacc tgcgccaaat cggtatgcag aaaaacctcg gcagcgggcg cgtcggcatc
     6001 ctgttttcgc acaaccggac cgaaaacacc ttcgacgacg gcatcggcaa ctcggcacgg
     6061 cttgcccacg gcgccgtttt cgggcaatac ggcatcggca ggttcgacat cggcatcagc
     6121 acgggcgcgg gttttagcag cggcagtctt tcagacggca tcggaggcaa aatccgccgc
     6181 cgcgtgctgc attacggcat tcaggcacga taccgcgccg gtttcggcgg attcggcatc
     6241 gaaccgtaca tcggcgcaac gcgctatttc gtccaaaaag cggattaccg ctacgaaaac
     6301 gtcaatatcg ccacccccgg tcttgcgttc aaccgctacc gcgcgggcat taaggcagat
     6361 tattcattca aaccggcgca acacatttcc atcacgcctt atttgagcct gtcctatacc
     6421 gatgccgctt cgggcaaagt ccgaacacgc gtcaataccg ccgtattggc tcaggatttc
     6481 ggcaaaaccc gcagtgcgga atggggcgta aacgccgaaa tcaaaggttt cacgctgtcc
     6541 ctccacgctg ccgccgccaa aggcccgcaa ctggaagcgc aacacagcgc gggcatcaaa
     6601 ttaggctacc gctggtaacc gccggatatg ccgaaagggg tctgacgatg ccaccgtgcg
     6661 ctgtcaaacc ctttttctgc cgccgccttg tgcctgctcc attgtctgat aaccgtcaaa
     6721 ttgattttta gcccatgttt ggtgggtcgg ggaaatctat atcttcgtcc gtgccgaaat
     6781 ag
//
LOCUS       DAWKQB010000111         6774 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.111, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000111 DAWKQB010000000
VERSION     DAWKQB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.111"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(452..1012)
                     /locus_tag="WJL14_001621"
     CDS             complement(452..1012)
                     /locus_tag="WJL14_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ7656670.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1429..2469)
                     /gene="mutY"
                     /locus_tag="WJL14_001622"
     CDS             complement(1429..2469)
                     /gene="mutY"
                     /locus_tag="WJL14_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ7656671.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVEQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLLITPFEAQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2632..3715
                     /locus_tag="WJL14_001623"
                     /pseudo
     CDS             2632..3715
                     /locus_tag="WJL14_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3971..4609)
                     /locus_tag="WJL14_001624"
     CDS             complement(3971..4609)
                     /locus_tag="WJL14_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ7656672.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4790..6625)
                     /gene="edd"
                     /locus_tag="WJL14_001625"
     CDS             complement(4790..6625)
                     /gene="edd"
                     /locus_tag="WJL14_001625"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ7656673.1"
                     /translation="MNPTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVHEGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
BASE COUNT         1521 a         1612 c         1915 g         1726 t
ORIGIN      
        1 ctgattaaag tgagtgcgca aagccacctg aaagcgatgt gtttgaacct gttgaaagcg
       61 gccaacaggc taagtgcgcc cgctgccgcc taaaaggcgg cccggatgcc tgattatcgg
      121 gtatccggag aggattaagg ggggatttgg gtagaattag gcggtatttg gggcgaaaac
      181 agccgaaaac agctgaaaac agctgaaaac ctgtgtttgg gtttcggctg tcgggaggga
      241 agggaatttt gcaaaggtct caaatagtac ggaatcgatt cacttggtgc ttcagcacct
      301 tagagaatcg ttctctttga gctaaggcga ggcaacgctg tactggttta aatttaattc
      361 actatactgc tgaagtcgtg aattaaatgc taaatatcta gcaaaaaagt gcggtcaaaa
      421 tttaccgcac ttttcgtttc gaacaatcga attatttaat cacgccacat gccatacgtg
      481 ggccgccacc gccaagtgga gctggatgat cggagtgatt atcaccaccc gtgtggatca
      541 taatagagtg accgcgaaca tcatctaaat gtttaagacg tggtgctaaa acaggatttg
      601 ttgctgtgcc atcatgcaat acagttaatg caggtaaatc acctaagtgt gcatcatctt
      661 gccatgggta accatgttgt tttgcacctt taggatccca gtgaccgcct gcgcctaaac
      721 cagctgtcaa tttaccttct ttttcttttg gctcacagct tgggttttca tggatgtgga
      781 aaccatgtaa gccttcgctt aatccttgta aatcaggggt aaacacaaga ccatagttag
      841 attcagtaat agtcactgta cccacatctt tgttaccgtt tactggatca agttgttgca
      901 ctttcacttc aatagaagca cctttaggta tcgtattatg ctcgtgtgct tgcgcaacac
      961 taactgaaca tactgcacta accgctagtg ctaataaggt tttcatattc attgcaatct
     1021 cctttattgc agtaaaaact cgcgactatt ttagtcaatt ttgttttaaa agccgataaa
     1081 tttttacgaa aattttacat taattgttat ttgcagaaaa ttagaacccg ttatcacgtc
     1141 ttggataaat gccaaaacca ttgtgattcc gagaattaaa aagaaaaatt tcggaatcct
     1201 catccttttc tgaaacctga ttgcggtatc catggctttg tctttgtcat ttaagtttgt
     1261 gttcatgttt ttgaccttct ttgcttttta aatccttttt aaattgccga cttcttccgt
     1321 tccaaatgtc ccatttgaac attatcccgc cgcctaaaat acgacccaaa ttcatcggtt
     1381 gttcttgctg cgatacctgc aaacaacagt tcaatgggtt tattttgttt attctaacct
     1441 attaccgttt aaataaattt ccaaaggctt gggcaaaccg taatctttca aatgcgccgg
     1501 ctttatccaa atgccgtctg aagggctttc agacggcatt tgtgcttcaa agggcgtaat
     1561 caatagcagc cggtgcgtca ggcggtgggt cagggcggtt tgttcgtcca tatctgccat
     1621 ggtcagggag aatttggcgg caaagtcgga aagcccgttc aaactttcaa aacacggcac
     1681 gcaatacagc ccgccccaaa tgcctttggc ggggcgtttt tccagcaaaa tcgcgccgtc
     1741 ccggttgcgg acaatcagcc agtaaagcgg cagggtcggt acttcggcgg cggttttttt
     1801 gcgcggcagc tcggcggtgc ggttttgctt tttcgcttcg cagatgtccg ccatcgggca
     1861 ttggtggcac aagggtttcg tccgtttgca cacggtcgcg cccaaatcca tcaaaccttg
     1921 tgtataggca ggcatatcgg cgttttcaga cggcagcagg ctttcggcaa gtgtccagag
     1981 cgagttttca aattttttgt cctgcggatt gccgtcgcgg gcgaacacgc ggcagagtac
     2041 gcgtttgacg ttgccgtcca aaatggtttc gcggcggttg aaggagaagg cgcaaatggc
     2101 ggcggcggtg cttctgccta cgccgcagag ggtttccaag tctttgcgct ccgacggaaa
     2161 cgtgccgccg aattgttcga cgacttgttg cgcggctttg tgcaggttgc gcgcgcggct
     2221 gtaatagccc aagcccgccc acaacgacaa cacttcgtct tgcggcgcgg cggcaagcgt
     2281 ctgaacggtc gggaattttt ctaagaaacg cggatagtag tccaacacgg tggcgacctg
     2341 cgtttgctgg agcatgattt cggaaagcca gacgcaataa gggtttttga cctgccaagg
     2401 gaggtggtgg cgaccgtgtt gtttttgcca gcggatgagc cgttcggaga aggggatggg
     2461 tgtgttcatt aatatcaatc ggtggtttta tttatattta aaacagtatg ttattgtata
     2521 acattgtgaa aataattctt attgacttat tttttgtagg ggcatataac tcatataagg
     2581 aaactttatt gcggtattga aattatttat caacaagcaa ggagtatcag catgaaagca
     2641 atggtttatc acggcgcaaa cgacatccgt tttgaagaaa aaccccgtcc gcagattatc
     2701 gatccgaccg atgcggtggt gaaaatcgtc aaaaccacga tttgcggtac cgatttgggt
     2761 atttggaaag gcaaaaaaac ccgaagtcgc cgacggccgt attctcggtc atgaaggcat
     2821 cggtattgta gaagaagtcg gcgaggctgt aaaaaacatc aaagtcggcg ataaagtcat
     2881 tatttcatgc gtcagcaaat gctgtacttg cgacaactgc aaaatccaac tttattcgca
     2941 ttgccgcaac ggcggctgga ttttgggcta catgattgac ggcacgcagg cagaatacgt
     3001 ccgcacgcag gcagaatacg tccgcacgca ggcagaatac gtccgcacgc cttatgccga
     3061 caacagcctc gtgccgctgc ccgacaacgt caacgaagaa gtcgccctgt tgttgagcga
     3121 cgctttgccg accgcacacg aaatcggcgt gcaatacggc gatgtcaaac ccggcgacac
     3181 cgtattcatc gcaggtgccg ctcctgtcgg tatgtccgcc ttgccgaccg cccaactgta
     3241 cagccccgcc gccatcattg tttgcgatat ggacgaaaac cgtttgaaac tggcgaaaga
     3301 gttgggcgca acccatacca ttaaccctgc ttccggcgat gtaagcaagc aagttgccgc
     3361 tatcgtcggc gaagacggcg tggactgcgc gatcgaagcc gtcggtatcc ctgcaacttg
     3421 gaatatgtgc caagacattg tgaaacccgg cggccatatc gccgttgtcg gcgtacacgg
     3481 tcaatccgtt gatttcaaac tggaaaaact gtggattaaa aagcttgcca tcactaccgg
     3541 cctggtaaac gccaatacca ccgaaatgct gatgaaggca atttccagca gctccgtcga
     3601 ttacaccaaa atgttgaccc accgtttcaa attcagcgaa ttggaaaaag cctacgacgt
     3661 gttcaaacac gccgccgaaa atcaagcgat gaaagtggtt ttggaagcgg attaatcggc
     3721 gttgaatcag ttgtttttta agcggatgcc gtctgaatgc gtttcagacg gcattttttc
     3781 agccgcgtag tatgggctgt ccggctgctg tcgaatgaac tgaacgttat ttttattatg
     3841 cggaaggcgg atgggcattt taggggcagt atgtcggagt ggtgctgccg tgtatgccga
     3901 ctgcgccgcc aagcggcaaa atgccgtctg agactggaaa gggcttcaga cggcattttt
     3961 acgatgcggt tcaggctttg ggtttcaatg ccgccgcttc tttggcgaga cgggtgatgg
     4021 tgtcccagtc tttgtttttc acggcttctt tcggcgtcag ccatgagccg ccgacgcaca
     4081 ggacgttggg cagtgccaag taatcgggcg cggtggcgag gctgatgccg cctgtcgggc
     4141 agaagcgaac atcggcgtaa gggccgtaca gggctttgag catggctttg ccgccaacga
     4201 cttcggcagg gaagagtttg agggtgtcga tgccgtgttc caaagccagt tggatttcgc
     4261 ccggggtggc aacaccggga atcaggggga tgccgctgtt gtggccggct ttggcgaggg
     4321 attcgtgcaa acccgggctg atggcgaaaa ccgcgcctgc gtcttcgacg gctttgagct
     4381 gttcagggtt ggtaaccgta cccgcgccga cgatggcgtt gggcacttct ttggcaatca
     4441 ggcggatggc atcgaggccg acaggggtgc gcagggtgat ttcgagggtg gggatgccgc
     4501 cttcgacaag ggcgtgggac aaatcgacgg cagtactcaa gtcgtcaatc gccattaccg
     4561 gtacaactgc gccggcggtc aggatttcgc ggggggtcag tttggacatt tcggttctcc
     4621 gggtgggatg gggtatttta ttaagatggg acaggttgta ggtcggattc aagaaaccga
     4681 catcttggac ggctgtgtcg gattcgagaa tccgacctac gtctatctta aaacacttga
     4741 ataatttttc agacggcctt cggtctgaaa aggccgtctg aaacgcgcat caggcaaatt
     4801 cgccgccgaa gctcatcgcg ccggtttcgg cgctgctggt catgctgcgg aagttggcga
     4861 aaagttcgcg gccgcagcct tgttggttcg cgcccaagtc gatgctttcg acttctcggg
     4921 cgttccattc ggcttcgtta atcaggacgt tgagttcgcc ggtaacggag tcgaagcgga
     4981 tcaggtcgcc ggtacggatt ttggcgatgt tgccgcccat cagggcttcg ggtgtcatgt
     5041 ggatggaggc tggaactttg ccggacgcgc cggacatacg gccgtcggtc agcagcgcca
     5101 ctttgaagcc gcggtcttgc aggatgccta aaggcggggt cagtttgtgc aattcgggca
     5161 taccgttggc acgcgggcct tggtagcgga cgacgcacac aaaatcgcgt tccaactcgc
     5221 cgcgttcaaa cgcagccaac acttcgcgtt ggtcgttgaa cacgatggca ggcgcttcaa
     5281 taatgcggca gccttcgtgc acggcggaca ctttaatcac gccacgtccg atgttgcctt
     5341 tcatcaggcg cagaccgccg tcgggggaga acgggttgtc agctttacgc aggatgtcgt
     5401 cgttgccgct ggtttcgggg gcttcgcgcc attcgagttt gccgtcgatg aggaaaggct
     5461 ctttggtgta gtggcgcata ccgtgtccga cgacggtatc gacatcgtcg tgcaacaggc
     5521 ctgcattcag caattcgcgg ataacgaaag gcagtccgcc cgctgcggta aagtggttca
     5581 cgtcggcctt gccgttcgga taaacgcgaa tgagcagcgg gatgatggag gaaatttcgt
     5641 cgaagtcgtc ccagttcaaa atcacgccgg cggcgcgtgc catggcaacg aggtgcatgg
     5701 tgtggttggt cgaaccgccg gttgccatca ggccaatcaa ggcgttgata aaggattttt
     5761 cggtcaacat ttcgcccaaa ggtttaatcg tgccgttttt gatgccgcgc gcgaggtgtc
     5821 cggcggcgta gcgggttagg gcttcgcgca ggtcggtgta agggtggacg aaggcggcgg
     5881 caggcaggtg cacgcccatc atttccatca tcatttggtt ggagtttgcc gtgccgtaga
     5941 aagtgcaggt gcccgggctg tggtaagaac ccatttcgct tttcaaaagt tcgttgcgtc
     6001 cgaccttgcc ttcggcgaaa agctggcggg tgcgggcttt ttctttgttg ccgataccgc
     6061 tggacatcgg gcctgcgggg acgaagatac ccggaatatg accgaacgaa agcgcgccta
     6121 tcatcaaacc cggaacgatt ttgtcgcata cgcccataaa caggctgccg tcaaacattt
     6181 gatgcgacag cccgacggcg gtactcatgg caatcacgtc gcgggagaac agcgacagtt
     6241 ccataccggc gtagccttgc gtgatgccgt cgcacatggc gggcgtgccg ccggcgactt
     6301 gggcggtcgc gccgtttttc tgcgcttcgt ctttgatttg gtcagggaag tctttaaacg
     6361 gctggtgtgc ggaaaccatg tcgttgtagg cggtgatgat gcctaagttg gggacggttt
     6421 cctgaagcat ttcgattttg atacttttag gcatggcagc atagccgtgt gccaagttgc
     6481 tgcagccgag ctggttgcgc tccaagcgtc ccatttgttt ggcgctgcgg attttcgcca
     6541 gatatttttc acgcgtcgga cggctgcgtt caataatgcg cccggtgatt tcggcgagtt
     6601 tggagtggat aggagtgggg ttcatgttcg gtctcctgtc ggaagtgggt ggtatgggga
     6661 ttttgtttgc gcgtttctga ttttttgtat tgggtgtggg gaatttggcg tttcagacga
     6721 cctttcaaat cttacaaggg gtcgtctgaa aacctcacta tagtggatta actt
//
LOCUS       DAWKQB010000112         6733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.112, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000112 DAWKQB010000000
VERSION     DAWKQB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.112"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WJL14_001626"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WJL14_001626"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ7656674.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WJL14_001627"
     CDS             1102..1512
                     /locus_tag="WJL14_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ7656675.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLAANGLEMAGGGDCGDFSCLFQKSGSRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WJL14_001628"
     CDS             1533..3596
                     /locus_tag="WJL14_001628"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ7656676.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAIAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARRLNATVAADEALLDEVTALVEYPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="WJL14_001629"
     CDS             3613..4659
                     /locus_tag="WJL14_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7656677.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHG
                     KIWKKPIRHEDIAAFFPFGNPIRNNTMIMRRSVIEGGLRYDTECDWAEDYQFWYEVSK
                     QGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGVWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4701..5540
                     /locus_tag="WJL14_001630"
     CDS             4701..5540
                     /locus_tag="WJL14_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7656678.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMWFFLDRFAALPSEGLHPVDWMMFGNPDDRERMPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRCLNSKQQRRDSPANTFKHRLIRALTKISREREKRRQRREQLIGKIIV
                     PFQ"
     gene            5552..5689
                     /locus_tag="WJL14_001631"
     CDS             5552..5689
                     /locus_tag="WJL14_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEZ7656679.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLIKNRRQARSDSAGKEQE
                     I"
     gene            5689..6495
                     /locus_tag="WJL14_001632"
     CDS             5689..6495
                     /locus_tag="WJL14_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ7656680.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHFGMAGYIISR
                     KAMRFFLDRFAVLPSERLKAVDWMLFSSFLDKGGMTVCQLTPALCVQSETLPSQLKNG
                     RQESYRNRRSPKVLLKRALGKIGREIERARERKRQKKLEKHLGRHVVPFE"
BASE COUNT         1682 a         1859 c         1777 g         1415 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgcaggtac ttcccacccc gccacctgcc
      241 tgcgcgcact cggccccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccca
      301 aagacggccg ctacggcgac aaccccaacc gcctgcaaca ttattaccaa ttccaagtcg
      361 ccctcaagcc cgctcccgcc aatatccaag acctctatct cgactctctg cgcgaattgg
      421 gcatcgatcc caaagtccac gacatccgct ttgtcgaaga cgactgggaa aaccccaccc
      481 tcggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctatttcca acaagtcggc ggcatcgact gcacccccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgct ggacggtaat acagtaactt acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgactg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgaagaaaa cgccgccctc gccctgcctg
      841 cctacgagct ggtcctcaaa gcggggcata cgttcaacct tttagacgca cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaacgccg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gaggacgatg ccgaagcgtt cgaccgcttt
     1261 atggacgagc tgattgcgta tcttgcggca aacggcttgg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccggctccc gaggcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggt cgcgaagatg tcgccacttt gcacgccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagcga aacctataaa
     1501 caggcagatt aaccaaattc aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccattgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt taaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa acaggatgtg tacgcctacg aatacgtcca aaccggcaaa ccgctgggcg
     1921 aacttttgga agacattatc aatcaagccg ttaagaaact gccgattccg aaagtgatgc
     1981 gttggggcag cagcacgttt accttcgtgc gccctgttca cgggctgatt gtgctgcacg
     2041 gcggcgacat cgtaaacgtc agcgttttgg gtctgcaaag cagcaatcaa accttgggac
     2101 accgcttctt gtccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgaacaaa
     2161 tgcgcgggca aggcaaagtc gtcgcttcgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaagg gcaggcgcgc cgtctgaacg cgaccgttgc cgccgatgaa gcattgctgg
     2281 acgaagtaac cgcgctggtc gaataccccg ttgttttgga agccggtttt gaggaacact
     2341 tcctcgccgt gcctcaagaa tgcttgattt tgacgatgca gcaaaaccaa aaatacttcc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtctcc aacctgcaaa
     2461 ccgcagaccc gtcgcacatc atccaaggca acgaacgcgt tttgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaaa caagaccaaa aggcgacttt ggaaagccgc ctgcccaagc
     2581 tggcaaacgt ggtttatcac aacaaaatcg gttcgcaagc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gctaaagcgt tgggcgcaga tgccgccgcc gccgaacgcg
     2701 ccgcgcgtct ggcaaaagcc gacttggtaa ccgaaatggt cggcgagttc cctgaattgc
     2761 aaggcaccat gggcaaatac tatgcccgtt tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgataaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg cttgctcaac gagcaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgttgccgcc gtattggcga
     3181 aacagccgcg ccgtttggac gacgtagtgg cgaaactgca agccgttgcc gcgttcaaac
     3241 aactgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaat ctgctgaaaa
     3301 aagccgatgc cgagttgggc gcggttaacg aaagcctgct gcaacaggac gaagaaaaag
     3361 ccctctttgc cgccgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaagcc gcaagtcgat gccttctttg
     3481 acggcgtgat ggtgatggcg gaagatgccg ccgtaaaaca aaaccgtttg aacctgttga
     3541 accgtttggc agagcagatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagcctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg agattttgat tgtcgatgac
     3781 ggctcgacag acggtacgct tgccattgcc aaggattttc aaaagcggga cagccgtatc
     3841 aaaatccttg cacaagctca aaattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcattgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcac
     4081 cacgaacacg gcaaaatttg gaaaaagccg atccggcacg aagacatcgc cgcctttttc
     4141 ccttttggca accctatacg caacaacacg atgattatgc ggcgcagcgt cattgaggga
     4201 ggcttgcgtt acgatactga atgcgattgg gcggaagatt accaattttg gtacgaagtc
     4261 agcaaacagg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcatccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gccgcccgcc
     4561 ggcgtgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcaccg attgctgaaa aaccgttgaa aaacgccgct ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttagcttcc gccgcagaac
     4741 gcagggcgca cattgccgca accttcggcg cgcgcggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg caatggcgga actcgtaccc ggtttgtcgg
     4861 cgcaccccta tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 aacaggcatt ggacgaaggt ctgccgtata tcgccgtatt tgaagatgat gtcttgctcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctactgcggg cgtgcctttc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttcccga aaggcgatgt ggtttttctt ggacaggttt gccgccctgc
     5221 cgtctgaagg gctgcacccc gtcgattgga tgatgttcgg caaccctgac gacagggaaa
     5281 ggatgccggt ttgccagctc aatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgctgc ctgaacagca
     5401 aacagcaaag gcgcgattcc cccgccaaca cattcaaaca ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgtc ggcaaaggcg cgaacagtta atcggcaaga
     5521 tcattgtgcc tttccaataa aaggagaaaa gatggacatc gtatttgcag cagacggcag
     5581 gatgcggcgg ctgtttacct tgaggcaata cttcggcatt ttacgccggc tgattaaaaa
     5641 ccgccggcag gcgcggtcgg attcggcagg gaaagaacag gagatttaat gcaaaaccac
     5701 gttatcagct tagcttccgc cgcagaacgc agggcgcaca ttaccgatac cttcggcgtg
     5761 cgcggcatcc cgtttcagtt tttcgacgca ctgatgccgt ctgaaaggct ggaacaggca
     5821 atggcggaac tcgtacccgg tttgtcggcg cacccctatt tgagcggagt ggaaaaagcc
     5881 tgctttatga gccacgccgt attgtggaaa caggcattgg acgaaggtct gccgtatatc
     5941 gccgtatttg aagatgatgt cttgctcggc gaaggcgcgg agaaattcct tgccgaagac
     6001 gcttggctgc aagaacgctt tgaccccgat tccgccttta tcgtccgctt ggaaacgatg
     6061 tttatgcacg tcctgacctc gccctccggc gtggcggact acggcgggcg cgcctttccg
     6121 ctgttggaaa gcgaacattt tggtatggcg ggctatatta tttcccgaaa ggcgatgcgt
     6181 ttctttttgg acaggtttgc cgttttgccg tctgaacgcc tgaaggcggt ggattggatg
     6241 ctcttcagca gttttctcga caaagggggg atgacggttt gccaactgac ccccgccttg
     6301 tgcgtccaaa gcgaaaccct gcccagccaa ctgaaaaacg gcaggcagga atcgtaccgc
     6361 aaccgccgct ccccgaaggt tttgttgaaa cgcgctttgg gaaaaatcgg cagggaaatc
     6421 gaaagggcga gggaacggaa acgtcagaaa aaattggaaa aacacctcgg gcggcacgtt
     6481 gtcccgtttg aataggtttt ggcctttcct tatttggcgg gtgtgcgctg tccctaaatt
     6541 tatgtcgggg aatccggttt gttcggtttc acttgttttc gggtaagccc taattcgtga
     6601 ttcccgagaa aacagaaagc caaaaacaga aacctaaaat ctcgtcattc tcgcgcaggc
     6661 gggaatctag gtctttaaac ttcggttttt tccgataaat tcttgccgca ttaaaattct
     6721 agattcctgc ctg
//
LOCUS       DAWKQB010000113         6559 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.113, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000113 DAWKQB010000000
VERSION     DAWKQB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.113"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            304..1737
                     /locus_tag="WJL14_001633"
     CDS             304..1737
                     /locus_tag="WJL14_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ7656681.1"
                     /translation="MNEYSQLIKHPDISLSPISDGIGVGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTESRGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLIVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALVVLAYEAGVPQDLLPVVSGRASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSGIYDAFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1846..2634
                     /locus_tag="WJL14_001634"
     CDS             1846..2634
                     /locus_tag="WJL14_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ7656682.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEKDRINVVTAAEVAVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            3002..4558
                     /locus_tag="WJL14_001635"
     CDS             3002..4558
                     /locus_tag="WJL14_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7656683.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4575..5321
                     /gene="surE"
                     /locus_tag="WJL14_001636"
     CDS             4575..5321
                     /gene="surE"
                     /locus_tag="WJL14_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ7656684.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGMPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGEVSDREEGTDFGEC
                     EAGFITVTPLQIDLTAYRDMAETAVFWHTD"
     gene            5424..6431
                     /locus_tag="WJL14_001637"
     CDS             5424..6431
                     /locus_tag="WJL14_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7656685.1"
                     /translation="MLKQTTLLAACTAVAALLGGCATQQPAPVIAGNSGMQTVPSAPV
                     YNPYGATPYNAAPAANDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNMLSIGQIVKVKPAGYAAPKTSAVE
                     SRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGI
                     VWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHN
                     SSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTEASRTQLHFEVRQNGKPVNPNSYIA
                     F"
BASE COUNT         1512 a         1691 c         1846 g         1510 t
ORIGIN      
        1 gttcggattt ttgccatact ctaccacacg ttgcaactgc aatctttgct ccttatatat
       61 tgcaccccat caaagggctg cattactttt ctttctaaag attactatcg tataaaaaat
      121 atttttctta aacgacaggg attgcgcccg cgccatattc agacaccgcc cggatgttgc
      181 gctgcccgat cggatgcttc agacggcatc ggaagggttt gcagttttgg aatatgaaga
      241 tgataatgtc cgcgagattg acggcatttg aaagcggtta ttccgcatac cggaggatac
      301 gaaatgaacg aatattccca attaatcaag catcccgata tttccctttc cccgatttca
      361 gacggcatcg gggtcggcaa tccggcaacg ggcgaaattt tggcgtatgt ccgcaatacg
      421 ggttcggaca aactgaaaaa cctgattcaa aaggcggcgg cagcgcaaaa attatgggcg
      481 gcgaaaactg ctttggaacg cgccgacata ttgtggcgtt ggtatttttt agttaaagaa
      541 aacaaagaag cactggcgcg cctgatgacg atggagcagg gcaaaagcct gaccgagtcg
      601 cgcggcgaaa tcgattatgc ggcttcgttt gtgcgctggt ttgccgaaga agcgcggcgg
      661 attgacggcg atgtgttaac gagtgtaaaa gcgtcgcaaa aactgattgt gttgaaacag
      721 cctgtcggcg ttaccgccgc gattacgccg tggaacttcc catctgcaat gattgcgcgc
      781 aaggctgcgc ctgctttggc ggtgggctgc gcgatgatcg tcaaacccgc atcgctcacg
      841 cctttgagtg cgtatgcgtt ggtcgtgctg gcttatgaag cgggcgtacc acaggatttg
      901 ttgcccgtcg tcagcggtcg cgcatcggaa atcggccgtg aatttgccac gaacccgatt
      961 atccgcaaaa tcagcttcac cggctctacc gaagtcggtg cgaaaatttt tgccgacagc
     1021 gcggcggaca ttaaaaaact cagtttggag ctgggcggca acgcgccgtt tatcgtgttt
     1081 gacgatgccg atttggacaa agccgtcgaa ggtgcgctcg ccagcaagtt ccgcaacagc
     1141 ggtcagacct gcgtctgcac caaccgcgtt tacgcccaat cgggcattta cgacgcattt
     1201 tgccgcaaat tgagtgaaaa agcagccgcg ctcaaattgg gcaacggctt ggaggatggc
     1261 gtaaaccaag ggccgctgat tgaggaaaaa gcggtagaaa aagtcgagca gcacatcgcc
     1321 gacgcgctct caaaaggcgc ggtctgtctg accggcggca aacgcagcgc gttgggcgga
     1381 acgttttttg agccgaccgt cttaagcggc gtaacggcgc aaatggcggt agctcgcgag
     1441 gaaaccttcg ggcctttgtg tccggtattc cgttttgaaa ccgaagccga ggtcatcgag
     1501 gctgcgaaca atacggaata cggtttggca gcttaccttt tcaccgccga caccgcccgc
     1561 caatggcgcg tcggcgaagc cttggaatac ggcatggttg gcatcaatac gggcttaatc
     1621 agtaatgaag cggcaccgtt tggcggcgtg aaacgcagcg gtttgggacg tgaaggcagc
     1681 aaatacggtg cggacgaata tctagaattg aaatatctgt gtatggatgt cgggtgacgg
     1741 atgccgtctg aacggcaggg tttcagacgg cgccgcattt taagcagtct ctatctgttg
     1801 tacaatgcgc cctgttttta cggttatttt tgatttgaac aagatatgat ggaaaacgga
     1861 aaaacagtcc cgagatggcg ttttgccttg aaaagtgcgg gctggcacct cttaatcagc
     1921 ctgtcggttg cagggctggc ggcattgctg gtttttaagg tttggtatcc ttatccttat
     1981 gccgagctga cgggagggct gtcgctttat cagctggtgg tggctgtcga tattgtatgt
     2041 ggtccgctgc tgactttaat tttggcaagc ccgaagaaaa agacaaaggc acgcatggtc
     2101 gatttttcca tggtcggcat catccagctg gcggctttgg tgtacggtct gcacagcgtt
     2161 tctttggcgc gtcccgtggt ggaggcgttt gaaaaagaca ggattaatgt tgtgaccgcc
     2221 gccgaagtgg cggttgaaga cctgcacaaa gcgccggaag ggctgcaaag cctgtcgtgg
     2281 ttcggcatcc gccgcattgc attgaaagaa cctgaggatg cggatgagaa gaacaagacg
     2341 ctggatttgt ccctgaaagg tatcgagccg agtatgcgtc ccgaccggtg gctgccgtat
     2401 tccgacaagg aagcagaaga aatccgcaaa catctgaaac cgctgaaagt cttggcggat
     2461 gcgagaaaaa cgacggttgc ggacattctg aaacaggcag gtctcgccga aggggaggag
     2521 ctgtattacc tgccgtttac cagcagcagg cagaaagagt ggatagtcat taccgataaa
     2581 gagggcaaca ccaaaggcta cgcgccgata gacggcttca tcatcacccc ttaagcgttg
     2641 ggactccgtc cgcactcgaa catccgttct tcgcggcggt agaatcagac tgtatttgag
     2701 aggggcgcaa cccctcaatc agggcaacac ataggggaag gctttatgtg ccgtccgtgt
     2761 gttgaaacat ttcaaaataa gctcatgccg tccgaacatc ctttcagacg gtatggcgtt
     2821 ttgccattgc cccgataagc tgttaaacta tttaaattat ttcgaccgaa ggtacacgca
     2881 catgcaagac cccgagcaga gcagcaagcc cgcccgccgt tttttgtgcg taccgtctgt
     2941 tcaggcagct tgagcgcttc aggctgcctt ttcgcgtttg aacttgagga atacgaaaat
     3001 catggatttc agttggttgg cagaaccgca tacctggata ggttttgcca cgcttttggt
     3061 gttggaagtc gtattgggga tagacaatct tgtctttgtg gcgattttgg caaacaaggt
     3121 ccagcccgca cggcgcgacc gagcacggat tatcgggctg gggctggcag tcgtcatccg
     3181 catcattatg cttgctttta tggcgcacat catcacgctg accgagccgc tgttccaaat
     3241 cggcggcctc gccgtttccg gcaaggacat gattatgctc gcgggcggta ttttcctgct
     3301 ttacaaagcc accaccgaac tgcatgaacg cctcgaaggg cacaaccgtt ttaccgttgc
     3361 cgacaaccaa aaaaaacacg cgccgttttg gggcgtggtc gcgcaaatcc tgatactgga
     3421 tgccgtgttt tccattgatt cggtcattac tgcggtggcg atggttgacc atatcgtcgt
     3481 ggcgatgggt gcggtcgtcg tcgcgatggc tgtaatgatt tctgccagca aactcttgac
     3541 cgaatttgtc gacagacacc ctaccgtcgt gatgctctgc cttggttttt tgttgatgat
     3601 cggtttcagc ctgattgccg aagccttcca tttccacatt cccaaaggct acctctacgc
     3661 cgccatcggc ttctcgattt taatcgaact gtttaaccag atttcccagc gcaacagccg
     3721 caaaaacgac tacatcggca gctcgtggcg caaacggact gctgaaaacg ttttgggtat
     3781 gatgggcata cgcgaaagcg tgcttgccga cgcgggcggc gaatccgggg acgacgcgca
     3841 ttttgaagaa aacgaaaaat cgatgatacg cagcgtgctg acgcttgccg aacgcccgat
     3901 tatgggggtg atgatcccac gccgcgacat cgaacggctg gacatttccc aaagccgcga
     3961 agaacagtgt gcccaactgc aaaacacgcc ttacagccgc ctgctcgttg tcggtaaggc
     4021 gggcgtggac gaacctttgg gctacatcaa caaaaaagac ctgctgtccc aactgctgga
     4081 aacaggcggt ctcgacattc agacggcatt gcgtcagccg ctcgttttgc ccgacagcac
     4141 caccgcgctg ggcgcaatcg aactcttccg ccaaagcagc gcggattatg ctttggtggt
     4201 ggacgagttc ggcgcggtat tgggcatggt aaccatgaaa gacctgctcg aaaccatcgc
     4261 aggcgagttc cccgaagaat ttgagcgcga agaagaaccc gccgttcagg ggaatcccga
     4321 tgaaagcctg acagtggaag gcgcgttgga atatgtggag ctggcatccc aactcaatct
     4381 gccgcagcag caggaagatg ccgatttcca tacggttgct gggctgatta tggaagaatt
     4441 gcaaaccatc cccgatgtcg gtgattttgc cgatttccac ggctggcggt ttgaagtggt
     4501 cgaaaaagaa gggcagcgca tcgagcgggt caaaatcacc aaattgcccg aagaataagc
     4561 attcaggata gaaaatgaac gttttgattt ccaacgacga cggctacctc tccgaaggca
     4621 tcgccgtttt ggcgcgcgtt acggcggaat ttgccaacgt cagggtggtc gcgcccgaac
     4681 gcgacaggag cggggtcagc aattcgctga cactggaacg ccctttgcag ttgaaacagg
     4741 cgcaaaacgg cttttactat gtcaacggca cgccgaccga ctgcatccac atcgggcagt
     4801 ctgtattttc ggattttcag gccgattttg tcttttcggg catcaaccgg ggcgcgaata
     4861 tgggggacga cacgctttat tcggggacgg ttgcggcggc aaccgaagcc taccttatgg
     4921 gcatgcccgc cgtggcgttt tccttaaacg acgcttccgg acgctattgg gcaaccgcag
     4981 aacaggcact gtggacattg ttggcgcatt ttttcaaaaa ccccccgcag tcccctattt
     5041 tgtggaacat caatatcccc gccgtcgcgc cggaagatgt acggggcatt aaaatcgccc
     5101 gtttgggcag gcggcaccac ggtcagaacg tcatccccgc gcgcaatccg cgcggcgaac
     5161 agatttattg gataggaccg gtcggcgaag tttccgatcg ggaagaggga acggatttcg
     5221 gcgaatgcga agcgggtttc attaccgtaa cgccgctgca aatcgatttg accgcttatc
     5281 gggacatggc ggaaacggcg gtcttttggc atacggactg accgttttat caaataccgt
     5341 cgggctttgc gcttgagaag aacgcgcatt tttattatga tatatcgcat tcaggctata
     5401 tatcggaaca tgaaggttat cttatgttga aacaaacgac acttttggca gcttgtaccg
     5461 ccgttgccgc tctgttgggc ggttgcgcca cccaacagcc tgctcctgtc attgcaggca
     5521 attcaggtat gcagaccgta ccgtctgcgc cggtttacaa tccttatggc gcaacgccgt
     5581 acaatgccgc tcctgccgcc aacgatgcgc cgtatgtgcc gccggtgcaa agcgcgccgg
     5641 tttatacgcc tcctgcttat gttccgccgt ctgcacctgc cgtttcgggt acatacgttc
     5701 cttcttacgc acccgtcgac atcaacgcgg cgacgcatac tattgtgcgc ggcgacacgg
     5761 tgtacaacat ttccaaacgc taccatatct ctcaagacga tttccgtgcg tggaacggca
     5821 tgaccgacaa tatgttgagc atcggtcaga ttgttaaagt caaaccggca ggatatgccg
     5881 caccgaaaac ctcagccgta gaaagcaggc ccgccgtacc ggctgccgtg caaacccctg
     5941 tgaaacccgc cgcgcaaccg cctgtgcagt ccgcgccgca acctgccgcg cccgctgcgg
     6001 aaaataaagc ggttcccgcg cccgccccgc aatctcctgc cgcttcgcct tccggcacgc
     6061 gttcggtcgg cggcattgtt tggcagcgtc cgacgcaagg taaagtggtt gccgatttcg
     6121 gcggcaacaa caagggtgtc gatattgcag gaaatgcggg acagcccgtt ttggcggcgg
     6181 ctgacggcaa agtggtttat gcaggttccg gtttgagggg atacggcaat ttggtcatca
     6241 tccagcataa ttcttccttc ctgaccgcat acgggcacaa ccaaaaattg ctggtcggcg
     6301 aaggccagca ggtcaaacgc gggcagcagg tcgctttgat gggcaatacc gaggcttcta
     6361 gaacgcagct tcatttcgag gtgcggcaaa acggcaaacc ggttaatccg aacagctata
     6421 tcgcgttctg actttctgcc tgaaatcaag ttggaaatgc cgtctgaaag gttttcagac
     6481 ggcatttttg tttttggcgg ctgtgttttc aaattattcg gaagggatgg ggcatataaa
     6541 tttgtttgac tgtgtttgg
//
LOCUS       DAWKQB010000114         6549 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.114, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000114 DAWKQB010000000
VERSION     DAWKQB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.114"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WJL14_001638"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="WJL14_001638"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ7656686.1"
                     /translation="MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTIVKSRLTVEPKR
                     VGEPLVLDGSAKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMVDANGINLDQFALWYSQAGTPVLDAQGRL
                     KNNVFELTIKQTVPPTPDMADKQPMMIPVKIGLLNCNGEAVAFDYQGKRATEAVLLLT
                     EAEQTFQFESVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGVELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2847..3641)
                     /locus_tag="WJL14_001639"
     CDS             complement(2847..3641)
                     /locus_tag="WJL14_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ7656687.1"
                     /translation="MTTILAFDIETVPDVQCIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWEAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(4390..5259)
                     /locus_tag="WJL14_001640"
     CDS             complement(4390..5259)
                     /locus_tag="WJL14_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ7656688.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5310..5846)
                     /gene="ruvC"
                     /locus_tag="WJL14_001641"
     CDS             complement(5310..5846)
                     /gene="ruvC"
                     /locus_tag="WJL14_001641"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ7656689.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5849..6088)
                     /locus_tag="WJL14_001642"
     CDS             complement(5849..6088)
                     /locus_tag="WJL14_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ7656690.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6118..>6549)
                     /locus_tag="WJL14_001643"
     CDS             complement(6118..>6549)
                     /locus_tag="WJL14_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7656691.1"
                     /translation="NIPVWVNGDITSPQKAQAVLKQTAADGIMIGRGAQGRPWFFRDL
                     KHYAEHGVLPPALSLAECTATILNHIRAMHAFYGDTAGVRIARKHIGWYIDEMPDGEQ
                     TRREINRLDSAAAQYDMFAGYLERLAEKTDSWACAYRPNAF"
BASE COUNT         1499 a         1699 c         1694 g         1657 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcctgc
      241 accaggcggg cggcgacctg cgggttaaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgagc gggctttgtt gggattttcg aggctgaact tcggatgctg caaggcggtt
      421 tgaacctgtt gcagggtgtc gctgcggcgg cttgagccga cgagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcggcgt atttctcggc aacggtttcg atgtgcgcgg gatcggcgcg caggacgaag
      661 gcgcggcaga cattgcgcag cgtgcgccaa ccggcggctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag aaagcggacg
      781 gcaagtatat ccaacaaggc ttcgcgcgcc tgatggtagc gcagcgggtc gatgttttcc
      841 gcgccgtccc acagctcggc ttcagacggc acacccaaaa gcagggcttt gaaagcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 aactcgacgc cgtctgaaag cgcggcaagg ttggcggcga cggcacggcg gtagagcgtt
     1021 tgtgcggctt cccagcgcgt gaaggcgtcg ctgtcatggg cgagcagaag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcacc ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgct ttcgaactgg aaggtctgtt cggcttcggt cagcagcaac
     1201 acggcttcgg tcgcgcgttt gccctgataa tcaaatgcca ccgcttcgcc gttgcagttc
     1261 agcagcccga ttttgacggg aatcatcatc ggctgtttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaac acattgtttt tcagacgccc ttgagcatct
     1381 aaaaccggcg tacctgcttg gctgtaccac aaggcgaatt ggtcgaggtt gatgccgttc
     1441 gcgtccacca tcgccgcgcg gaaatcgtcg caggtaacag cctgtccgtc gtggcgttgg
     1501 aaatagagct tcataccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatat
     1621 cgggcggggc gcaccggatg tgcggtcgga cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttttcgat acggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttccttc agcgaaagct ggaaccagtc gcggcaggtc
     1801 acgcggttgc ccgtccagtt gtggaaatat tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtgtt aaagatgttc
     1921 aaacccttgt tttccatcgc acccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcattgcgtt tttcagcgat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgtgaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacagat aactcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaatactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtccgcaat cgtcagcgtc tcgccttcca acacataatc cgccgccacg
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctctcccacc
     2581 ctcttcggct cgaccgtcaa acgcgacttc acaatggttt gcggttcgtt aatatcaaaa
     2641 tgtaaatcgg ttttgagaat atggtaggcg ggcgtttgat agtctttgag ataatgcacg
     2701 gttttgctca tttttttctt tcaatgttat tttgtttgac tggaaaaggc ttcagacggc
     2761 atgggcaaac cctgcaccga tatgccgtat gaaaccgaaa catcagtgcg gacgcaccgt
     2821 caaacaaccg gttttaattt caatctttat ttccacgctt ggacaaattc tgcccaatgc
     2881 ggtttgtctt ctgtttgggc ggagagatag tttctgatcc gcttgatttc catttcgtat
     2941 tcgtctgcgt cgaatctgcc gctgaccaga cagaaacgca gatacatcag ataggtattt
     3001 accgcatcgg tttcgcaata attgcggatt tccttcagcc tgcccgcgtg gaacgcctcc
     3061 cacaccttgc tgccgtccat acccagcttg cccggaaaac cgcacagttt cgccatatcg
     3121 tccaacggca cattcgccct cggctggtaa agcgcgagca aatccatcaa atcgcaatga
     3181 cgttggtggt agcggctgat gtagttgttc cacttgaaat cgcggctgtc gccgaaatcg
     3241 ccgtcgccca tatcccaata gcgcgcggcg ttgatgccgt gtagcagcga acggtaatgc
     3301 agaaccggca ggtcgaaacc gccgccgttc caactgacca gttgcggcgt atgtttttca
     3361 atcaactcga aaaatttggc gataaccact tcctcgccgt catccatctc gccgattgta
     3421 ccgacatgga ctttatcctg cccccaacgc atgcagcacg aaatcgccac aatctgatga
     3481 agatgatgct gcataaaatc cccacccgtc tgagcacggc gtttttgctg ggcaaacagc
     3541 accacttcat cgtcgggcag cgaggacggc aagtcataca gcgtacggat acactgcaca
     3601 tcgggtacgg tttcaatatc gaaagccaaa atcgtggtca tgacagcacc ttgtatttaa
     3661 aacagacttg cgcctattgt gtcattaaaa ggcggataaa aaaagaggca accccccaca
     3721 ggattgcccc aatacctcaa atcagagatt tacgcttcac aaacaataca ggctttcgcc
     3781 tgcggctttg cccgcgtagc tcaactctac gccggcaaac ttttgtttca ccgtttccga
     3841 tgaaaccccg accaatcgca agattgaccg gaaaatcctt tcagacggca tttcctgcct
     3901 gtcgtgtaat tccatgtagc gaaatgtacg ccattttcta cgctttgcca agcatttttt
     3961 acaatataaa tgtcaaaaca ttaattttat aaaattgctg aaaatattaa atatatgaat
     4021 ttttatttta aatattttaa taaatataaa tttaattttg atttatattt aaatttaagc
     4081 ataaaatgtc aaatattaaa ggaaatatga aaagcatata ttaaatattt atttataacg
     4141 ctatgttttt aaagaaaatt aattttaata tattaactag attgtctgca gatattcatg
     4201 ggtttgcggt atttcttcca aaacctgctt cgaattttcc gaccaagtct taaaaatatt
     4261 gtttttgaga tacttaaata gcagcgatta tcaaatgaaa tctgttcata taatctacca
     4321 tttcgcattt aaaaaaacaa tcaggagttt cgactcgaaa cgcctgatat gttttgtaat
     4381 tttacgtagt cagtaaaaat cggggctgcc ttccggacgg gttttgaaac gcttgtgcag
     4441 ccaaaaatac tgctcgggat gttcgcgcac gcgttcctcg ataaaacggt tcatgcgctg
     4501 cgcgtcggcc tgcgcatctt cactcggaaa ggattcccaa gcagggtaga aatgcaatgt
     4561 aaccgtattg tccgcctcgc ggacagggat ggcgggtatc acttttgcat ttgcaagcgc
     4621 ggcaatgcgg ctcaagccgg taatcgttgc cgtccgaata ccgaagaaat cgacaaaaac
     4681 cgaatcgttg cgtccgaaat cctgatcggg cagatacaga aacggcgcgc tgcttttgcg
     4741 gaactgtttg acgagggcgc gcagcccttc ggtgcgcccg ataaggaaaa cgttgtgata
     4801 gcggttgcgg cctttcaaaa tctgttcgtc caatatcttg tttttttggt gggaatacat
     4861 actgatcagc ggaacatcct gattgagcgc gtacaccgcc atctcgaacg cggtgaagtg
     4921 cggatacagg atgatgactt tttcccctgc cgccagagcg tcgtccaaat aatgtttgtt
     4981 gcggtagcgc accagtgatt tcaaacgccc ggcgggcgcg taccaatata aaccgtattc
     5041 caacatcagt ttcgccatat gtttgaaatg ctgtttcaac acggttttac gcttttttcc
     5101 gtcccactcg ggaaagcatt ttgccaaatt gatttcgccg atacggcggc ggggtttgac
     5161 caaaaggtag gcgagcaagc ctgtcagatc agcaagtttg tgcagcagcg caaacggcag
     5221 aaactgcaaa acatacagta caaaaaatat aaatttcatc tcgatacaca ttttcttttc
     5281 aggtggcaaa atacaaatgc cgtctgaaac tattgaaacc tgccgcgctt gacctgcatc
     5341 cccgaaggat tgagtttggc ggcaagcccg tggttacgta aggcgtgggt cagcgccacc
     5401 gccaaaccgt cggcggcatc cggctggggc gttcccgaaa gtcccaacat ctgcaccacc
     5461 atatgctgca cctgttcttt tgccgcctta cccttgccga ctaccgcctg tttgacctgc
     5521 aaggccgtgt attccgaaac gggcagctta tggctgacca atgccgccaa tgccgcgccc
     5581 ctagcctgac cgagcatcag cgtcgatgca gggttgacgt tgacgaacac ctgttccact
     5641 gccgcctgtt gaggcttgta aacagtaacg acttcgccga tatgccgcac aatcacggca
     5701 atcctgtctg ccagaggcgc atcggcaggc gttttgatgc aaccggaggc gacgtaaaaa
     5761 tgatcgcgcc ccctgacatc gatgacaccg aaacccgtta cgcggctgcc cgggtcgatg
     5821 cctaagatac ggacggttgc cgacatattc acaacaaacc gtgttgaatc agcttcttac
     5881 gcagggtatt gcggttcagc cccaacatga cggaggcttt ggactgatta ccgccgcatt
     5941 gctccatcac gcacaccagc agcggctttt ccacctgatg caataccata tcgtacacgc
     6001 cgcaaggttc ggtaccgttc aggtctttga aatattgttc taaattttgt ctgatgcatt
     6061 gagaaatatc gggaagggta tggggcatga ttgcactttc aaaggataat caagtgttca
     6121 gaaggcattt gggcggtagg cgcacgccca actgtcggtt ttttcggcaa gtctttcaag
     6181 ataacctgca aacatgtcgt attgcgccgc cgcactgtcc aagcggttga tttcacgacg
     6241 tgtctgttcg ccgtcgggca tttcgtcgat gtaccagcct atgtgtttgc gtgcgatgcg
     6301 cacaccggcg gtgtcgccgt aaaacgcgtg catggctcgg atgtggttca aaatagtggc
     6361 ggtacattct gccaaactca aggcaggcgg taaaacaccg tgttcggcgt aatgtttcaa
     6421 atcgcggaag aaccacggtc tgccttgcgc gccgcgccct atcataatgc cgtctgcggc
     6481 ggtttgtttg aggacggctt gggctttttg cggcgaggta atgtcgccgt tgacccagac
     6541 cgggatgtt
//
LOCUS       DAWKQB010000115         6487 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.115, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000115 DAWKQB010000000
VERSION     DAWKQB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.115"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..1465)
                     /locus_tag="WJL14_001644"
     CDS             complement(110..1465)
                     /locus_tag="WJL14_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ7656692.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            1662..2483
                     /gene="nadE"
                     /locus_tag="WJL14_001645"
     CDS             1662..2483
                     /gene="nadE"
                     /locus_tag="WJL14_001645"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ7656693.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(2553..3026)
                     /locus_tag="WJL14_001646"
     CDS             complement(2553..3026)
                     /locus_tag="WJL14_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ7656694.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAELLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQAPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            3329..3661
                     /gene="trxA"
                     /locus_tag="WJL14_001647"
     CDS             3329..3661
                     /gene="trxA"
                     /locus_tag="WJL14_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ7656695.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(3714..4661)
                     /locus_tag="WJL14_001648"
     CDS             complement(3714..4661)
                     /locus_tag="WJL14_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ7656696.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMRSEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            4989..6377
                     /locus_tag="WJL14_001649"
     CDS             4989..6377
                     /locus_tag="WJL14_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7656697.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFARLEVLILDEAD
                     RMLDMGFIDDIETIVEATPEDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDADIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGWRGDHKPSKEGFGGKTRGEGFKKEGFKRDGFKKTGEGFKGKRKAGDS
                     FAGKGERRYKDR"
BASE COUNT         1542 a         1750 c         1712 g         1483 t
ORIGIN      
        1 cggttttttg aatttcagtc atttctgata aaccccggcg gctttccgct tccggactcc
       61 cgcttttgcg ggaatgacgg cggaggggga gcggtttttc cgattgggtt taaatgcaat
      121 cgaacaaatc ctgctgccct tgttctttgc ttacgcgcac gtcggtttcg ccgtcggcga
      181 aggtaatgtg cagcttctgc ccctgcttca aaacatcggc gttgcggatg acttgtccgc
      241 gtgtgttttt gacgacggaa aagccgcgct ccagaatgtg ctgcggcgaa acggcttcga
      301 gcaatgcggc ttgggcggtc aggctttggc ggcggtgggt aagcagttgg ctgaaggcgg
      361 cgggcaatgc cgtctgaagg cggacgatgt tgtttttgca aacggaaaca tcaggacggt
      421 aatgttgcag ggcttgggtt tggcgttcaa aacgggcggt gtgggcacgg aggttttgct
      481 tcatcgagta agacagcgtt tgcgccagct tgccgattga agcgcgctgc tcatcaagtt
      541 tttggcgcgg atgacggatt tgccgcgcaa gccagtcgag tttttggctg gcatcgaaat
      601 agcgttgttc caaaacggtt ttcagacggc cttgcgcctg agcgagacga tgtagcgatt
      661 cttggcggtt ggggctgacc agctccgccg caccggtcgg cgtgggcgcg cgcacgtcgg
      721 cgacaaaatc ggcaagcgta aaatcggttt cgtgtcccac gccactgacg acgggaaccg
      781 tgcaggcttc gattgcgcgc acgaccggtt cttcgttaaa cgcccacaag tcttcaatgc
      841 tgccgccgcc gcgacagaca atcaacacgt cgcattcggc gcgttgcgag gcggttttaa
      901 tcgcttgggc aatttgtaac tcgctgcctg cgccttgaac aggtgtcgga taaacgataa
      961 cgggaatttc gggcgcgcgg cgtttcaagg tggtaacgac atcgcgcaat gccgctgctg
     1021 ccaggctggt gacgatgccg atacattgcg gacgggtagg caacggcttt ttgcgttccg
     1081 ccgcaaacgc gccttccgcc tgcaactgtg ccttcaaccg ctcgtaggct tcgtaaagct
     1141 gccccaaacc tttgagccgc acctcattca cggtaatttg aaattcgccc cgcgcctcgt
     1201 aaatactgat ttttcctgat acttcgatat ggtcgccttc tttcaaaggc ttcgccaaac
     1261 gcgccgccgc acctttgaac atcgcgcaac gcacctgcgc gcggctgtct ttgagcgaga
     1321 aataataatg tccgctggcg gcacgggtca ggttggacac ttcgcccgca atccacaggc
     1381 cggcaaggtg gttttccaaa agacttttgg caaatgcgtt caactcggaa acggacaaca
     1441 cgtcagaatg aaaaaaatca gacatagaat caatcaaata ataaaaaata tgaatatgtt
     1501 ttgaagccta aggcggtatt agtccgccta aattgtcaac aatattataa cacgcgccat
     1561 cttgccgccc gccttttccc gtatgacttt tttaagcggg gaatgggaaa aatattcatc
     1621 aacccgccct caatctattc aaattacacc gccgacaagc tatgatgcgg atattttcga
     1681 caggaggaaa aatggatacg caggcagtta tcacacatat tgtccgatgg ttggacgaat
     1741 acgccgcccg ggcaaatgca aaagggtttg tcgtgggcgt ttccggcggc atcgattccg
     1801 ccgtcgtctc cgcactcgcc gcccgcaccg gccgcaccac gctgcttctg gatatgccga
     1861 tacgccaaca ccccgaccag cttgaacggg caaggctgca catccgcaat ctgcaacggc
     1921 aatatgccaa tgtaagcgca caaacggtcg atctgaccga caccttccag acctttgaac
     1981 aaaccgtcgg cgttcatcag acggcatttg acaatcagcc gctttccctc gccaacgcca
     2041 gaagccgcct gcgtatgctg accctgtact actacgggca gatacacgga ctactggtta
     2101 cggggacagg taacaaaatt gaagatttcg gtgtaggatt ttttaccaaa tacggcgacg
     2161 gcggcgtgga catcagcccg attgccgacc tgaccaaaac ccaggtttac cggcttgccg
     2221 aagcattggg cgtggacgag gcgattcaaa aagccccgcc gaccgacggc ctgtgggata
     2281 cggaacgcac cgacgaagaa cagatgggcg caagctatcc cgaactggag tgggcaatgg
     2341 gcgtgtacgg cacgcgcaaa cccgaagatt ttgaagggcg gcagcgcgaa gttctggaaa
     2401 tctatacgcg gctgcaccgc gcgatgcagc acaaaatcaa cccgattccc gtgtgccgca
     2461 ttccgcccga attgttgggc tgaaacgtgg aaatgccgtc tgaagcggaa aaccgtgttt
     2521 cagacggcat tgacatatcc ggctcctatc ccttaagaat cgagtacgcg ggcaaacaaa
     2581 atatcgtttt ccaaatgaat gtggtcgttc aaatcctcca ccatttcttt ggccaaagcg
     2641 taaagccgcg tccagcttcc gcaggctcct tcgggcgctt ggaaattatc ggtcagctct
     2701 ttgagccttg cgatggcgcg gtcgtgttct tcatgttcgt gcatcatcac gctgatgggc
     2761 atcgccgcac cccgaccgac accctgatta atcatcggaa acagcatcct ttcctctttc
     2821 atcatatgca tcagcagttc gttctgcata taggcaagca gctcggcaat ttccgccgga
     2881 aaggtatcgg catgaacttg ggccactttt tgcgccagcg gcaccaattc ttcaaattgt
     2941 gcacggtgta cattgtggta gcgttgcagg atatgatcga cggttgcacc aaagggagca
     3001 gtttcccaaa cggaaaaatc agtcatggca gggttccttt tatttatagg gtttcgggtt
     3061 tggttttgaa cattcatact ttaagaatct atttaaacgg agcatacacc gcccgtgcgt
     3121 ttctgtacag cttcaaaaac attccttaca ttttgataat aaaagtaatt ttcaaaaata
     3181 aaatactgtc cgaaccgttt ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa
     3241 ctattatccc gcccgcccac ttgaaatttt tatgcccaag ccctatcctg cacgctatcg
     3301 tgccaatccc aaccgaaaag gaaaaataat gagcagcgaa ttgatcgtac acaccggcga
     3361 tgccgccttc gagcaagatg ttttgaaatc cgacctcccc gtcctgctgg acttttgggc
     3421 tccgtggtgc ggcccgtgca aaatgattgc cccgattttg gacgacattg ccgccgaatt
     3481 tgaaggccgt ctgaaagtag tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg
     3541 tttcggcgtg cgcggcattc cgaccctgat ggtgttcaaa aacggcgacg tcgtcgccac
     3601 caaagtcggc gcattggcaa aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg
     3661 ataaagcgca atcgaaaaag ccgccggaag attccggcgg ctttttcgca cccttaagat
     3721 ttgtggcgga tttcccagca ccgatggatt tttttgttgc ggaaatcttc gggaacggat
     3781 tgtttggaaa tgtctttgac ggcgtattgt tccgatacca agtcgtctaa gacgaagctg
     3841 cgcaggttgt tggaaaagta caaaatgccg tctgaagcga gcagcttcac cgcgccgtca
     3901 atcagctttt tgtgatcgcg ctgaatgtcg aggatgtcgg acattttctt gctgttggaa
     3961 aaactgggcg gatccatcac aatgaggtcg aactgcctgc cttccccata tgccgtctga
     4021 agatattgga acacgtcggc gcggacgatt ttgtgccgtt ccgtatcgat gccgttcaat
     4081 tcaaaattgc gtctcgccca atcgagataa gtgttggaca aatcgacagt ttcgctggat
     4141 gccgcgccgc cggtggcggc atagacagtg aagctgccgg tgtaggaaaa caggtttaaa
     4201 aaacgtttgc ccgccgccgt ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa
     4261 agccccgtat ccaaatactt atcaaggttg acccaaaact tgcggccgtt ttcggtgatg
     4321 acgaaatcgt cgcccgcctt gccggttttt tcgtattgct gcaaaccttt ttggcgttcg
     4381 cggcgtttga ggcggatttg ttcgggcgca aaaccggtaa cgaaggcgac ggcttccaag
     4441 acttcggcaa gccacgcttc gtattcttcg gaccgcatca gccagccggt atcgtattcc
     4501 tgaaggtgga tttggtcgcc gtaaacatcg acagcaaagg ggaattgggg gatgtcgcgg
     4561 tcgtaaatgc gccaggcttc gatgccgttg cgtttcgccc atttcataag gtgtttgatg
     4621 tttttgccca agcggttggc aaacggtgtg atgtcggtca ttggtttcag gcggaataaa
     4681 gtggaaaacg gcaattttac tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc
     4741 ctataccatc cgtcgcttat cttgtcatac gaagccatcg ccttccaacc taaaccgccc
     4801 ttacgggcgc gtttcttctg ttgctttgat tttgcaaagc gtatctgtgc aggttgccgt
     4861 cgatgtaaac cacaagcaag ccgcttgcga caaccctgta acttcacatt ccccgtatcg
     4921 ttacccttcc ctgcttcagg ccgtctgaac ctttcggacg cgagcgttgt tgtcttccaa
     4981 ggatagccat gtctattaaa tttgccgatt tgaaccttga taaaaacatt ttgtccgccg
     5041 tcagcagcga gggttacgaa agcccgacgc cgattcaggc gcaagccatt ccgtttgctt
     5101 tggaaggccg cgacatcatg gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc
     5161 tgttaccgac tttgcagcga ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgcg
     5221 cgttggtgtt gacgccgacc cgcgaattgg cggctcaagt cgagaaaaac gcgctggcgt
     5281 atgccaaaaa tatgcgttgg ttccgtaccg tcagcatcgt cggtggtgca tcgttcggct
     5341 accaaacccg cgccctgagc aaaccggtcg atctgattgt cgccacgccg ggccgtctga
     5401 tggacttgat gcaaagcggc aaagtcgatt tcgcccgctt ggaagtgctg attttggacg
     5461 aagccgaccg tatgttggat atgggcttta tcgacgacat cgaaaccatc gtcgaagcca
     5521 cgccggaaga ccgtcagact ttgttgttct ccgccacttg ggacggcgcg gtcggcaaac
     5581 tggcgcgcaa actgaccaaa gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag
     5641 gcaaaatcga agaacaactg ctgtactgcg acgatatgcg ccacaaaaac cgcctgctcg
     5701 atcatatctt gcgcgatgcc gacatcgatc aatgcgtgat tttcacgtcc accaaagcca
     5761 tgaccgaagt catcgcggat gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg
     5821 gcgatatgcc gcaaggctgg cgcaaccgca cgctgatgga tttgcgcaaa ggccgctgca
     5881 aaattttggt tgccaccgac gttgccgcac gcggtatcga cgtaccgacc attacccacg
     5941 ttatcaacta cgacctgccg aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg
     6001 gccgcgcagg ccgcacgggt attgcgatta cgtttgccga agtgaacgaa tacgtcaaag
     6061 tccacaaaat cgaaaaatac attaaccgaa aactgcccga actgaccatc gaaggcatgg
     6121 aaccgacccg caaacgcaaa tccgcaggcg gcaagccgaa aggcaaaggc ggctggggcg
     6181 atcgtaaatc cggcggttgg cgcggcgatc ataaaccgag caaagaaggc ttcggcggca
     6241 aaacgcgcgg cgaaggtttc aagaaagaag gctttaagag agacggtttc aaaaaaaccg
     6301 gcgaaggctt caaaggcaaa cgcaaagccg gcgattcttt tgcaggcaaa ggcgaacgcc
     6361 gttacaaaga ccgctaagcc ccaacctgcc gcataaacca atgccgtctg aaaccgattt
     6421 cgagtttcag acggcatttt tgcaatgttt cagcaccgcc cggctttgat acccaaagga
     6481 ttaatct
//
LOCUS       DAWKQB010000116         6306 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.116, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000116 DAWKQB010000000
VERSION     DAWKQB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.116"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..960
                     /locus_tag="WJL14_001650"
     CDS             <1..960
                     /locus_tag="WJL14_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7656698.1"
                     /translation="AEPHVPVSIPTATPLPKGEVTLSSDNGKIENINTTSTGSTSVIS
                     IQERAVTKNYFGVESQEKSFIFKTPGGAQYTLSSYADPIVPSYSSPDFKIPDRHAGQR
                     LADGSRIFICCSDSGATREAEITKQDYMKFGAWIGPNGEIDLFAGGFPVGKTPTSSSY
                     YGSSTPETQGKGKITYQVWGIRVKDGQFVTSSYTPPKGSSFTGYTNTPVLSFITANFN
                     SNKLAGEIRGNSDYGPSVKIENATISGPSFSGNATSGGKTGNLEGKFFGKFNGSYGNT
                     ETSIGGKITFKDDRSLDTVFGGVSYVKKLDETANRDTEHLTKQ"
     gene            988..3420
                     /gene="hupB"
                     /locus_tag="WJL14_001651"
     CDS             988..3420
                     /gene="hupB"
                     /locus_tag="WJL14_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ7656699.1"
                     /translation="MSIPFKPVLAAAAIAQAFPAFAADPAPQSTQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDDENNPRYAPLSDGTALVSSP
                     VWQNQNRSSAWVKGLEFNGTWNLDSIGLPQGTHAGVNVSYIKGKAKQTNGQETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNNTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4103..5737)
                     /gene="groL"
                     /locus_tag="WJL14_001652"
     CDS             complement(4103..5737)
                     /gene="groL"
                     /locus_tag="WJL14_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ7656700.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5830..6120)
                     /locus_tag="WJL14_001653"
     CDS             complement(5830..6120)
                     /locus_tag="WJL14_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ7656701.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
BASE COUNT         1540 a         1864 c         1519 g         1383 t
ORIGIN      
        1 gccgaaccgc acgtccccgt gtccatcccc accgccacgc cgctgcccaa aggcgaggta
       61 acgttatcaa gcgataacgg caaaatcgaa aacatcaaca ccaccagcac cggaagcaca
      121 tccgtcattt ccattcaaga gcgggcggtt acaaaaaact atttcggtgt tgaatcacaa
      181 gaaaaatcat tcatcttcaa aacacccggc ggcgcgcaat acaccctttc atcctacgcc
      241 gaccccatcg tccccagcta ctcctccccc gatttcaaaa ttcccgaccg ccacgcaggg
      301 cagcggcttg cagacggcag ccgcatcttt atctgctgca gcgactccgg ggcaaccaga
      361 gaagcggaaa ttaccaaaca agattatatg aaattcgggg catggatagg tcccaacggc
      421 gaaatcgacc tcttcgccgg cggcttcccc gtcggcaaaa caccgacatc aagcagttat
      481 tacggcagca gcacgcccga aacccaaggc aaaggcaaaa tcacttatca ggtttggggc
      541 atccgcgtca aagacgggca atttgttacc tcctcttata cgccgcccaa aggcagctct
      601 tttaccggct acaccaacac ccccgtcctt tcctttatca ccgccaattt caacagcaac
      661 aaactggcag gcgaaatccg tggcaacagc gattacggac cgagtgtgaa aattgaaaac
      721 gcaacgatca gcggtccgag cttttccggc aatgccacct ccggcggcaa aaccggcaac
      781 ttggaaggca agttttttgg aaaattcaac ggcagctacg gcaacactga aaccagcata
      841 ggcggcaaaa tcacttttaa agacgaccgc tccctcgata ccgtattcgg cggtgtgagt
      901 tatgtaaaaa aacttgatga aaccgccaat cgagataccg aacaccttac taaacaataa
      961 accatctcat ccctacaaag gtttcttatg tccatcccct tcaagcccgt attggctgcc
     1021 gccgccatcg cccaagcgtt tcccgccttt gcggcagacc ccgcgccgca gtccacccaa
     1081 acgctgaacg aaatcaccgt taccggcacg cacaaaaccc aaaaactcgg cgaagaaaaa
     1141 atccgccgca aaaccttaga caagctcttg gtcaacgacg aacacgacct ggtgcgctac
     1201 gaccccggca tttccgtcgt cgaaggcggc agggcgggtt ctaacggctt taccatacgc
     1261 ggcgtggaca aagaccgcgt cgccatcaac gttgacgggc tggcgcaggc ggaaagccgc
     1321 tcttccgaag ccttccaaga attgttcggc gcgtacggca acttcaacgc caaccgcaac
     1381 acttccgagc cggaaaactt ttccgaagtt accatcacca aaggcgcgga ctcgctcaaa
     1441 tccggcagcg gcgcattggg cggcgcagtc aattaccaaa ccaaatccgc aagcgattat
     1501 gtttccgaag acaagcccta ccatttgggg ataaagggcg gcagcgtcgg caaaaacagc
     1561 caaaaattca gcagcatcac cgccgccggc aggctctttg gtttggatgc cttattggtt
     1621 tatacccgcc gcttcggcaa agaaaccaaa aaccgctcga ctgagggcga tgtcgaaatt
     1681 aaaaacgacg gatatgtcta taacccgacc gatacaggcg gacccagcaa gtacctgacc
     1741 tatgtagcca caggggttgc gcgctcccaa cccgacccac aggaatgggt aaacaaaagc
     1801 accctgttca agctgggcta caacttcaac gatcaaaacc gtatcggctg gatttttgaa
     1861 gactcgcgca ccgaccgttt taccaacgag ctgtctaatt tgtggacggg tacgaccacg
     1921 tctgccgcaa cgggcgacta ccgccaccgc caagacgtga gctaccgccg ccgctccggt
     1981 gtcgaataca aaaacgaatt ggaacacggc ccgtgggaca gccttaagct gcgctacgac
     2041 aagcagcgca tcgatatgaa cacttggact tgggacatcc cgaaaaatta cgatacaaac
     2101 ggcatcaacg gtgaggttta ccattcgttc cggcatatcc gccaaaacac cgcgcaatgg
     2161 actgccgatt ttgaaaaaca actcgacttt tccaaagccg tttgggcggc gcaatacggc
     2221 ttaggcggcg gcagagggga caatgccaac tcggattaca gctatttcgc aaaactgtac
     2281 gcccccaaaa tcctcgcttc caaccaagcc aaaatcacga tgctgatcga aaaccggtcg
     2341 aaatataaat ttgcctattg gaacaatgcg tttcacttgg gcggcaacga ccgcttccgc
     2401 ctgaatgcgg gcatacgcta cgacaaaaac agcagcagcg cgaaagacga tccgaaatac
     2461 accaccgcca tccgggggca gattccccat ttgggttcgg aacgcgcgca cgccggcttc
     2521 agctacggca cggggttcga ctggcggttt accaagcatc tgcacttgtt ggcaaaatac
     2581 agcaccggct tccgcgcacc gacttcggac gaaacctggc tgctgttccc acaccccgat
     2641 ttctatctga aagccaaccc aaacctgaaa gccgaaaaag ccaaaaactg ggaattgggt
     2701 ctggctggca gcggcaaggc gggcaacttc aagctctcgg gcttcaaaac caaataccgc
     2761 gactttatcg aattgacgta tatgggcgtt tcgtcagacg atgaaaacaa ccccagatac
     2821 gccccacttt cagacggcac ggcattggtc agctcacccg tttggcaaaa ccaaaaccgc
     2881 tcctcagcgt gggtgaaagg tttggaattt aacggcacat ggaaccttga cagcatcggt
     2941 ttgccgcaag gcacgcacgc aggtgtcaat gtcagctaca tcaaaggcaa ggccaaacaa
     3001 accaacggac aggaaacccc gattaacgcc ctttccccgt ggacggcggt ttacagcctg
     3061 ggctatgacg cgccttccaa acgctggggc atcaacgcct acgccacgcg caccgccgcc
     3121 aaaaagccgt ccgacaccgt acacagcaac gacgacttga acaacccgtg gccttatgcc
     3181 aaacacagca aggcctatac gctgttcgac ctttccgcct acctcaacat cggcaaacag
     3241 gttacgctcc gcgccgccgc gtacaacatt accaacaagc agtactacac ttgggaatct
     3301 ttacgcagca tccgcgagtt cggcacggtc aaccgcgttg acaacaatac ccacgccggc
     3361 atccaacgct ttacctcgcc gggcaggagc tacaatttca ccatcgaagc gaagttctaa
     3421 gctcctgatg tgccaatgcc gtctgaaacc cgatgatttg ggtttcagac ggcatttttt
     3481 acagtcgcac cgccgcccct ttcaggcttg taccaagccc aatcccgcaa ctctccgtca
     3541 ttcccgcaac tctccgtcat tcccgcaact ctccgtcatt cccgcaactc tccgtcattc
     3601 ccgcgactct ccgtcattcc cgcgactctc cgtcattccc gcgactctcc gtcattccca
     3661 cggaagtggg aatctagaaa cgcaaagctg caagaattta tcggaaatga ctgaaactca
     3721 acgaacctgg attcccgcct gcgcgggaat gacgaagcta tccgcacaga aacctgcacc
     3781 acgtcattcc cacgaaagtg ggaatccaga acgtaaaatc tgaagaaacc gttttatccg
     3841 atacgtttcc gcaccgacag acctagattc ccgctttcgc gggaatgacg gcggaaaggt
     3901 tgctgttttt ccgataaatt cctgccgctc ttcgtttttg ggatggcggg aaataaaaca
     3961 aaagcgcgcg tatcaaaaaa caacaatgca aagaacggcg ttggcaattt taaggtttgc
     4021 gccttagccg cacttattcg caaaaaaaac cgcacggcgt tgaccgtgcg gcttgttgtc
     4081 tgaaggcttc agacggcatt gcttacatca tgccacccat accgcccatg ccgcccatat
     4141 cgggcatagc aggtttttct tcagggattt cggcaatcat gcaatcggtc gtcagcatca
     4201 ggccggcgat ggacgcggcg tgttgcagcg cggaacgggt tactttggcg gggtcgagta
     4261 cgcccatttc gatcatgtcg ccgtattcgc cggagcctgc gttgtaaccg tagttgcctt
     4321 tgccttccaa cactttgttc accaccacgc taggttcgcc gcctgcgttg gcaacgattt
     4381 ggcgcagcgg agactcaacg gcgcgcaaga cgatttgtac gcctgcgtct tggtcggcat
     4441 tgccggtgtg caggttttcc aaagcagcac gggcacgcaa cagggctacg ccgccgcctg
     4501 caaccacgcc ttcttcaacg gctgcgcggg tagcgtgcag cgcgtcttcc acgcggtctt
     4561 ttttctcttt catttcgact tcggtcgcgg caccgacttt gatgactgcc acgccgcctg
     4621 ccaatttagc cacgcgctct tgcagttttt ctttgtcgta atcgctggtt gcggtttcga
     4681 tttgttggcg gatttcggca acacgcgctt cgatttgggc tgcgtcgcca aagccgtcga
     4741 tgatggtggt gttttcttta ccgatttcga tgcgtttggc ttgacccaag tcgtccaaag
     4801 tcgctttttc caaagacaga ccgacttctt cggaaatcac cacgccgccg gtcaggatgg
     4861 cgatgtcttg caacatcgct ttgcggcggt cgccgaagcc aggggctttg acggcaacgg
     4921 ttttcaggat gcctcggatg ttgttcacga ccaaagtcgc caaggcttcg ccttctacgt
     4981 cttcagcgat aatcaacagc ggacggctgg cttttgccac ttgttccaaa acaggcagca
     5041 agtcgcggat gttgctgatt tttttgtcga acaacaatac aaacggattg tccaaagcag
     5101 cgatttgttt ttcagcgtcg ttgatgaagt aaggagacaa gtagccgcgg tcgaactgca
     5161 taccttcaac cacgtccagc tcgttttcca aagatttgcc gtcttcaacg gtaatcacgc
     5221 cttctttgcc gactttttcc atcgcttcgg cgataatcgc gccgacttgt tcgtcagagt
     5281 tggcggaaat cgagccgact tgggcgattt ctttggaagt gtcgcaaggt ttggcgatgt
     5341 tttttagctc ttcaaccaaa gcggcaacgg ctttgtcgat accgcgtttc aggtcggtcg
     5401 ggttcatacc ggcggtaacg tatttcatac cttcggcaac gatggattgc gccaatacgg
     5461 tggcggtagt cgtaccgtcg cctgccacgt cgttggtttt ggacgcgact tctttcacca
     5521 tttgcgcgcc catattttca aacttgtctt tcagttcgat ttctttggcg acggttacgc
     5581 cgtctttggt gatgtgcggg ccgccgaaag cgcggtcaac gactacgttg cgacctttag
     5641 gacccaaggt tacgcgcacg gcgtttgcca gaatgttcac gccgtttacc attttttggc
     5701 ggacttcatt gccgaattgt acgtcttttg ctgccatttc aattctccaa aaatcattaa
     5761 aactgtctga taaaaccgtt tatgccgtct gaaggcggtt tgccgtttca gacggcatcg
     5821 tgtccgtatt tatttttcaa cgataccgaa aatatcttct tcgcgcatta ccaacagctc
     5881 ttcgccgtcg gcttttacgg tttggccgct gtatttgccg aagatgattt tgtcgccgac
     5941 tttgacatcc agcggacggc gcgcgccgtc tttaccgatt ttgcccgcac ccacggcgat
     6001 cacttcgccc atatcgggtt tttcggcggc cgcacccggc aaaacgatgc ccgatgcggt
     6061 tttctcttca gcttccaagc gtttgacgac gacgcggtcg tgtaaaggac ggatggtcat
     6121 atttatgctc cgataaatgt tttgaaaaca atcatctgcc cgaacggttc ggacagattg
     6181 aagtggaaac cggacagccg tcaggcggct gcccgtataa gtcggcaaaa ttagggtgtg
     6241 tgcgggcaaa ttcaagtgag gcggaaaaaa tttatttccg ccgtttttta tagtggacta
     6301 aattta
//
LOCUS       DAWKQB010000117         6284 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.117, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000117 DAWKQB010000000
VERSION     DAWKQB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.117"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..2045
                     /locus_tag="WJL14_001654"
                     /pseudo
     CDS             1..2045
                     /locus_tag="WJL14_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            2287..3753
                     /locus_tag="WJL14_001655"
     CDS             2287..3753
                     /locus_tag="WJL14_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7656702.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(3954..4922)
                     /locus_tag="WJL14_001656"
     CDS             complement(3954..4922)
                     /locus_tag="WJL14_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656703.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            5000..>6284
                     /locus_tag="WJL14_001657"
     CDS             5000..>6284
                     /locus_tag="WJL14_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ7656704.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAEGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQ"
BASE COUNT         1457 a         1608 c         1902 g         1317 t
ORIGIN      
        1 gtcgggttaa tctattaaca ttatctgttt tatcgctgtt ttgcacgcca tatgtttgag
       61 gttcggatgc gtacgatccc gtcaaagaag ccgagattaa aaacaaattt attttagaag
      121 cggcggaaga cagaaattcc cacgtttggc gcggcccgtg cagcatatct tttgattgct
      181 tcggtatgtt cagagctcag cttggttcaa atactcgttc taccaaaatc ggcgacgatg
      241 ccgatttttc attttcagac aagccgaaac ccggcacttc ccattatttt tccagcggta
      301 aaaccgatca aaattcatcc gaatatgggt atgacgaaat caatatccaa ggtaaaaact
      361 acaatagcgg catactcgcc gtcgataata tgcccgttgt taagaaatat attacagata
      421 cttacgggga taatttaaag gatgcggtta agaagcaatt acaggattta tacaaaacaa
      481 gacccgaagc ttgggaagaa aataaaaaac ggactgagga ggcgtatata gaacagcttg
      541 gaccaaaatt tagtatactc aaacagaaaa accccgattt aattaataaa ttggtagaag
      601 attccgtact cactcctcat agtaatacat cacagactag tctcaacaac atcttcaata
      661 aaaaattaca cgtcaaaatc gaaaacaaat cccacgtcgc cggacaggtg ttggaactga
      721 ccaagatgac gctgaaagat tccctttggg aaccgcgccg ccattccgac atccatatgc
      781 tggaaacttc cgataatgcc cgcatccgcc tgaacacgaa agatgaaaaa ctgaccgtcc
      841 ataaagcgta tcagggcggt gcggatttcc tgttcggcta cgacgtgcgg gagtcggaca
      901 aacccgccct gacctttgaa gaaaaagtca gcggacaatc cggcgtggtt ttggaacgcc
      961 ggccggaaaa tctgaaaacg ctcgacgggc gcaaactgat tgcggcggaa aaggcagact
     1021 ctaattcgtt tgcgtttaaa caaaattacc ggcagggact gtacgaatta ttgctcaagc
     1081 aatgcgaagg cggattttgc ttgggcgtgc agcgtttggc tatccccgag gcggaagcgg
     1141 ttttatatgc ccaacaggct tatgcggcaa atactttgtt cgggctgcgt gccgccgaca
     1201 ggggcgacga cgtgtatgcc gccgatccgt cccgtcaaaa attgtggctg cgcttcatcg
     1261 gcggccggtc gcatcaaaat atacggggcg gcgcggctgc ggacgggcgg cgcaaaggcg
     1321 tgcaaatcgg cggcgaggtg tttgtacggc aaaatgaagg cagccggctg gcaatcggcg
     1381 tgatgggcgg cagggctggc cagcacgcat cagtcaacgg caaaggcggt gcggcaggca
     1441 gttatttgca tggttatggc gggggtgttt atgctgcgtg gcatcagttg cgcgataaac
     1501 aaacgggtgc gtatttggac ggctggttgc aataccaacg tttcaaacac cgcatcaatg
     1561 atgaaaaccg tgcggaacgc tacaaaacca aaggttggac ggcttctgtc gaaggcggct
     1621 acaacgcgct tgtggcggaa ggcgttgtcg gaaaaggcaa taatgtgcgg ttttacctgc
     1681 aaccgcaggc gcagtttacc tacttgggcg taaacggcgg ctttaccgac agcgagggga
     1741 cggcggtcgg actgctcggc agcggtcagt ggcaaagccg cgccggcatt cgggcaaaaa
     1801 cccgttttgc tttgcgtaac ggtgtcaatc ttcagccttt tgccgctttt aatgttttgc
     1861 acaggtcaaa atctttcggc gtggaaatgg acggcgaaaa acagacgctg gcaggcagga
     1921 cggcgctcga agggcggttc ggcattgaag ccggttggaa aggccatatg tccgcacgca
     1981 tcggatacgg caaaaggacg gacggcgaca aagaagccgc attgtcgctc aaatggctgt
     2041 tttgatgcgc cgggaaatgt tttgacgtac aggcggcaca ccggcacggc accgcgcgcc
     2101 gccccgcaaa ccaatccgaa ccctgccgtc ccgaaaggcg gggcataatg atgaaaccgg
     2161 cggaaaaccg ccggtttttt gccgccgttt gaaacccgat tctggcttca gacggcattg
     2221 tcgcggcatc gggcggcagg gtttggaaca gcggcataaa aaactgatac aatccgccgg
     2281 ttgataatgg ttatttttta tttttgtggg aagacattta tgcctgcacg aaacagatgg
     2341 atgctgctgc tgcctttatt ggcaagcgcg gcatatgccg aagaaacacc gcgcgaaccg
     2401 gatttgagaa gccgtcccga gttcaggctt catgaagcgg aggtcaaacc aatcgacagg
     2461 gagaaggtac cggggcaggt gcgggaaaaa ggaaaagttt tgcagattga cggcgaaacc
     2521 ctgctgaaaa atcccgaatt gctgtcccgc gcgatgtatt ccgcagtggt ctcaaacaat
     2581 attgccggta tccgcgttat tttgccgatt tacctacaac aggcgcagca ggataagatg
     2641 ttggcacttt atgcacaagg gattttggcg caggcagacg gtagggtgaa ggaggcgatt
     2701 tcccattacc gggaattgat tgccgcccaa cccgacgcgc ccgccgtccg tatgcgtttg
     2761 gcggcggcat tgtttgaaaa caggcagaac gaggcggcgg cagaccagtt cgaccgcctg
     2821 aaggcggaaa acctgccgcc gcagctgatg gagcaggtcg agctgtaccg caaggcattg
     2881 cgcgaacgcg atgcgtggaa ggtaaatggc ggcttcagcg ttacccgcga acacaatatc
     2941 aaccaagccc cgaaacggca gcagtacggc aaatggactt tcccgaaaca ggtggacggc
     3001 acggcggtca attaccggct cggcgcggag aaaaaatggt cgctgaaaaa cggctggtac
     3061 acgacggcgg gcggcgacgt gtccggcagg gtttatccgg ggaataagaa attcaacgat
     3121 atgacggcag gcgtttccgg cggcatcggt tttgccgacc ggcgcaaaga tgccgggctg
     3181 gcagtgttcc acgaacgccg cacctacggc aacgacgctt attcttacac caacggcgca
     3241 cgcctttatt tcaaccgttg gcaaaccccg aaatggcaaa cgttgtcttc ggcggagtgg
     3301 gggcgtttga agaatacgcg ccgggcgcgt tccgacaata cccatttgca aatttccaat
     3361 tcgctggtgt tttaccggaa tgcgcgccaa tattggatgg gcggtttgga tttttaccgc
     3421 gagcgcaacc ccgccgaccg gggcgacaat ttcaaccgtt acggcctgcg ctttgcctgg
     3481 gggcaggaat ggggcggcag cggcctgtct tcgctgttgc gcctcggcgc ggcgaaacgg
     3541 cattatgaaa aacccggctt tttcagcggt tttaaagggg aaaggcgcag ggataaagaa
     3601 ttgaacacat ccttgagcct ttggcaccgg gcattgcatt tcaaaggcat cacgccgcgc
     3661 ctgacgttgt cgcaccgcga aacgcggagt aacgatgtgt tcaacgaata cgagaaaaat
     3721 cgggcgtttg tcgagtttaa taaaacgttc tgattgctgt tccttttcgg aggaaaccct
     3781 gccggcggcg gtatcacggc gggcatcggc ggctttcggg cggtgctttg cgtgccgccg
     3841 cgtgtgcgga aacgcattcc ggtttttccg gcataacggc gatgcgaggt aaaatgccgt
     3901 ctgaaacccg attcgggctt cagacggcat tgtcgcggtt gcggcgggcg ggttcaccag
     3961 attccgtcaa aggttttcgc gccgcgccaa aatttccacc tgtcggcggg tttgaaggtc
     4021 agcgtaccgc cgtgttgtcc gtccgtggtg atgtccagcc gtttgatttt gccggtacgg
     4081 acggcttcgt agatcggtgc gaaccagtgt tcttcccact gctgtaaaac cgatgcatac
     4141 cgctccctgt cccctgtcag ggcggtcagg cgcaaatcgt ccataaacag gatatggtgc
     4201 gtgtcgggca ggtgtgccgc cgtttcttca taggcgcgga agttgtcggg caatgcgcgg
     4261 cggtcggggt ggaaacggct ccaaaccgta tcggcgaaaa gcgtgccgcc ttgactgctt
     4321 ccgtgcagac tgtcccaaag ccataagccg ttcaactcgg gcagcccgcg tttcttgcgg
     4381 ttatggttga cggggtgcgc cgccagccac atttggattt cggtttggac gcgcagccat
     4441 tccaacgcgt cttccccgtc cggttgatcg tccgcaccca acagtccgcc caagtccaaa
     4501 acgggcttcg cgccccagcg gtacgcgcgg ggaagggaaa ccagccataa ttcgggcagg
     4561 acgggaacga aacgccacgg aatatcgccg taaaacgccg acagatcgcg gcagagccgt
     4621 tccgcttcat ccgtaccgac atccaaatat tccgccgtca gcacatttgc ctgatgcatc
     4681 cccatctttt gccagacggg cgtggcgagc gcgacggttt cagacggcat attcaggctt
     4741 tgcgccgcgc gttccgccag tctgccgcac cacaaataac gcgcgtaaaa tgccgaagcc
     4801 gtgcagcttt ggcggtgcag cgagccgtat tgcaggattt tgttgaaggc gggcaggcag
     4861 agaggtattc gggtttcgtc ttcatccaaa ttgagcgagg gaatggcgag ggtgagtttc
     4921 atcgtttgac gtttcagaaa tgcaggtcag gcgcaacatt atagaggatt cggcgcaaac
     4981 gccgtcaaaa aggaacaata tggctgtctt cccactttcg gcaaaacatc ggaaatacgc
     5041 gctgcgcgcg cttgccgttt cgattatttt ggtgtcggcg gcatacattg cttcgacaga
     5101 gaggacggag cgcgtcagac cgcagcgcgt ggaacaaaaa ctgccgccgc tgtcttgggg
     5161 cggcagcggt gttcagacgg catattgggt gcaggaggcg gtgcagccag gcgactcgct
     5221 ggcggacgtg ctggcgcgtt cgggtatggc gcgggacgaa attgcccgaa taacggaaaa
     5281 atatggcggc gaagccgatt tgcggcattt gcgtgccgac cagtcggttc atgttttggt
     5341 cggcggcgac ggcggcgcgc gcgaagtgca gttttttacc gacgaagacg gcgagcgcaa
     5401 tctggtcgct ttggaaaaaa aaggcggcat atggcggcgg tcggcttctg aggcggatat
     5461 gaaggttttg ccgacgctgc gttcggtcgt ggtcaaaacg tcggcgcgcg gttcgctggc
     5521 gcgggcggaa gtgcccgtcg aaattcgcga atccttaagc gggattttcg ccggccgctt
     5581 cagccttgat ggtttgaagg aaggcgatgc cgtgcgcctg atttacgaca gcctgtattt
     5641 ccacgggcag caggtggcgg cgggcgatat tctggcggcg gaagtcgtta agggcggcac
     5701 aaggcatcag gcgttctatt accgttcgga caaggaagga ggagggggcg gcaattatta
     5761 cgatgaagac ggcagggtgt tgcaggaaaa aggcggcttc aacatcgagc cactggtcta
     5821 tacgcgcatt tcttcgccgt tcggctaccg tatgcacccc atcctgcaca cttggcggct
     5881 gcacacgggc atcgattatg ccgcaccgca gggaacgccg gtcagggctt ccgccgacgg
     5941 cgtgattacc tttaaaggcc ggaagggtgg ctacggcaac gcggtgatga tacgccacgc
     6001 caacggtgtg gaaacgctgt atgcgcactt gagcgcgttt tctcaggcag aaggcaatgt
     6061 gcgcggcggc gaggtcatcg gttttgtcgg ttcgaccggg cgttcgacgg ggccgcacct
     6121 gcattacgag gcgcgcatca atgggcagcc cgtcaatcct gtttcggtcg cattgccgac
     6181 acccgaattg acgcaggcgg acaaggcggc gtttgccgcg cagaaacaga aggcggacgc
     6241 gctgcttgcg cgcttgcgcg gcataccggt taccgtgtcg caat
//
LOCUS       DAWKQB010000118         6210 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.118, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000118 DAWKQB010000000
VERSION     DAWKQB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.118"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..826)
                     /gene="pglH"
                     /locus_tag="WJL14_001658"
     CDS             complement(<1..826)
                     /gene="pglH"
                     /locus_tag="WJL14_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ7656705.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPRFRSKTTRLF
                     YLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYLP"
     gene            complement(846..1382)
                     /locus_tag="WJL14_001659"
     CDS             complement(846..1382)
                     /locus_tag="WJL14_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656706.1"
                     /translation="MLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDN
                     LKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYD
                     TPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVNTLVRQPLLREAMGKRLHAHVA
                     ALCSDDEIYRTTMAAYTM"
     gene            complement(1334..1918)
                     /locus_tag="WJL14_001660"
     CDS             complement(1334..1918)
                     /locus_tag="WJL14_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656707.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVNFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPRNFLSGKPSKAASCSARCRVWTPSAPCI"
     gene            complement(1915..3336)
                     /locus_tag="WJL14_001661"
     CDS             complement(1915..3336)
                     /locus_tag="WJL14_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ7656708.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANAPPARLSATAESAAALLAAALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
     gene            complement(3531..4640)
                     /gene="ribD"
                     /locus_tag="WJL14_001662"
     CDS             complement(3531..4640)
                     /gene="ribD"
                     /locus_tag="WJL14_001662"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ7656709.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAHGSQVVGQG
                     FHVKAGEPHAEVHALHQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPPVAGKGFAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETADGK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGGGSGLFCLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNGNAF"
     gene            complement(4672..5136)
                     /gene="nrdR"
                     /locus_tag="WJL14_001663"
     CDS             complement(4672..5136)
                     /gene="nrdR"
                     /locus_tag="WJL14_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ7656710.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            complement(5165..6007)
                     /locus_tag="WJL14_001664"
     CDS             complement(5165..6007)
                     /locus_tag="WJL14_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ7656711.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT         1273 a         1576 c         2054 g         1307 t
ORIGIN      
        1 agggtaaata accgtaaaac ttgatggcgc gattttcata ttgtttgagt ttctccaaat
       61 cgggtcctcc gcccataatg tgcaattcga cgttttcgcc gtcgtctaaa agtttccgaa
      121 cacctttgca cacggtttcc acgtcgtagc tgtaactgag cgtgcccaag tagaaaaggc
      181 gggtggtttt ggagcggaat cgcggggggg cgatggcggc aaaatccgta ccgatataga
      241 cggcttcgcc gggtacgttc ggattggctt ctttggcgcg gtcgagataa gtctgcgata
      301 cggcaaccag cgcgtcggcg cagcggtagg cacggttggc gcgtgaagca aagggcagca
      361 gcttgtgcgg tacttttttc aaaaatggta cgaccgagga gaaagactcc ggccatacgt
      421 cctgcacatc gacaatcagt ttgtagccca aacgcgcttt gtgtttgccc aacagcaggt
      481 tggtcgccat cagcggatag gcagaataga cgatgtcctg ttccgccgcc tgcgggctgt
      541 gcagccagcg tgccaaattg cggacgaata cgcggtggct ggcgacgcgg gcgagggaga
      601 cgttttttcg gtagccaggt tcgtccagca gcttgacgcg cagcctgccg tttgaagtgg
      661 cggcagcatc ttcatgccgt ctgaatgatt tgtcgtagtg gcggaaacgg ctggtaatca
      721 gcaacacgtc gtgcgattgc gacaatcgtt ctgccagata ccaaaagcgg ttgaaatacg
      781 gttcggacgg cagcgagcaa taaggggcga ctatggtgat gttcatcggc ggctttcaga
      841 cggcattaca tggtatacgc cgccatggtg gtacggtaga tttcgtcgtc ggaacagagt
      901 gcggcgacgt gtgcgtgcag ccttttgccc attgcttcgc gcaacagcgg ttgtctgacg
      961 agcgtgttta cggcggcgat aaaggcttct tcgtcgccga aggggatgca gtaacccgtt
     1021 tccccgttga cgaccatttc ggaaatgccc gccatatcgt aggtaacgac gggcgtgtcg
     1081 taaagtccgg cttctaagat gttgttgccc acgcctgcgc cgtggtcgcc gatgcagtgg
     1141 ggcgtgttca ccaaaatatc cacgtctttg aagtaggcgg tcaaatcgcg cacgccgccg
     1201 aggaaggtaa ctttgccgtc tatgcccaga cgttccgcct gcgctttcaa attgtccatt
     1261 tcttcgccta tgcccgccac gttcaggcgt acgggcatat cgcggtcgac catttttttg
     1321 aaaatgtcca gcatcagatg cacggcgcgg acggtgtcca gacgcgacag cgtgccgagc
     1381 atgacgcagc ctttgacggt tttccagaaa ggaaattccg gggggggggg agtgcgttgt
     1441 aggcgtagac aatccggtcg gcggggaaac cgtggcggat gagtttttcg cgttcgtgtt
     1501 tgcagttacc gatgatgtac acgcccagct tgtcgaagag tttggcgatt ttggggtagg
     1561 ttgccgcgtc caaaccgtgc gagtggtaga tgattttggt tttcggcgaa acgattttgg
     1621 cggcaagggc gcaggcgggt acgacgcgcg ccatttggca gtcgatgatg tcgggttgtt
     1681 cgcgccgcag cattcgggcg taggcaatca tacctttgag gtagccgaaa aggccgccgc
     1741 gatagaaatt gacgggttgc caacgtatgc ccaaggcttg cgcctctttg agcaacggtc
     1801 cgtccgaaga ggcaagcatg atgtcgtgtc cgtgctgcct gagtagcctg ccgaggcgga
     1861 cggtggcggt ttccgtgccg cccaagcctg ccatagatgt ggtcaaaacg attttcataa
     1921 tgggaaacct tgttttttca aataatgaaa cagtttgtgc aaatctttcc ggtggcgcag
     1981 gatgcagcct gccagatata ccgcccatac gccggcaaac agggggtagt ttgccggagt
     2041 gccgaagcag gtgtaggccg ccgaggaggc caggcagaac aatgtgtgca tataaagcgg
     2101 caggcgtttg agcggctgcc acaggcggca ggagctttcg gtcttgaaaa caaaaaacag
     2161 ccaaaatgag gcggcacagg caaccgccgc gccgcgcgcg ccgccggacg gtacggcaag
     2221 ccccagcagc agcaggtttg ccgccagcgc gcccaaggtg gcgagcgcga tcgggcgcgt
     2281 tttgcggacg acgttcaaac cgatgccgct gatttctacc agcgtgcaaa acagcggagg
     2341 cagcatacac gatacgacga taaaccggac ggcggcgtag ttttccggca gcaggaggga
     2401 ggcgaggggc gagaaaatgc cggtcaggca gagggcggcg gcaagcaggg cggcggcgga
     2461 ttctgccgtt gccgagaggc gggcgggcgg ggcgtttgct tcgattgcgc ggaaaatata
     2521 cggtgtccag accgttgaaa agatgctttg gaacaataat gccgctccgc cgaacgaaat
     2581 acccatcgaa taaacgccga gctgttctag gccggcatat tttttcagga acaaacggtc
     2641 ggcggatgcc agcccccaat aggcgatgct gcttagtgcg atcggtatgc cgtagcgcag
     2701 gccgcgatgc aggacggcgg atgaaaacgg tgcgcgccgg acggccttca gacggcatcg
     2761 gttttgaaac agcaaaaagg cggcggcggc aaggtttgcc agcgcgtaaa cggcggtcag
     2821 gacggcggtg ttcgccggaa agtgcagcag cccgaccgtc agcggcagca gcagcaggat
     2881 ggcgagcttg gacacgagtt gcgcggacga aaaggcaagg gcgcgtcctt ccatacgcaa
     2941 aaccagtaag agaaagcgga tgggcaggaa gctcagttca aacagcacca gcccgatgcc
     3001 ggcggcggca tcgtcgagcg aaaacaggat ttcagacggc agggacgggc gggaaagcag
     3061 cagggcggct atcgcggcgg cagacagcag cggcggcagg aacagggttt tgaacaaagt
     3121 gtctttgtcg gcggcggcat agtattcgcg gacgtatgcc tgatccagcc cgaggcacaa
     3181 taccgacacc gtcagtcccg ccgccgtctg catcagcacg atgcgcccga tgtcgtcggc
     3241 agggaaatac cacgacagca gcggcaggat gatgacggct aaaaccgcgc tgccgatcga
     3301 gcctgccgcg tagccgagga tttcttttgt gtccattttt gatgtccggt cggcagcggg
     3361 attctgcctg tgtcgtctga agcctttctt gatcggaatt tgacggcttt caggccgtcg
     3421 cggctgccgg cggggtgcgg cagtccgggt tgcgcttttt cgggtgggcg gcggtctgaa
     3481 tggtgccgtt ttttatcggc gttattatat agtgaaatgg cgcaaaccct ttaaaaggcg
     3541 ttgccgtttt ttcggaacac ggttttgatg tcgtgtccga ggatttcgct tgaaacaggt
     3601 gtccacaagg gcggtgcgga aagggcggcg cggttttcgg gcaggcaaaa taaaccgctg
     3661 ccgccgccga ggattttggg cgagcggtat aaaacgattt cgtccgccaa atcttctgcc
     3721 aaaaatgcgg atgtgagttc ggagcctgct tcgaccatga tttcgccgaa accttcgtcg
     3781 gcaaggaggc gcatcaggtg gtgcaggtcg attttgccgt ctgccgtttc agacggcatc
     3841 aggatgcgga tgtgtgcgtg ttcccgatag gggcggagtt tatcttcatc gcgttccaag
     3901 gtggcgatgt aggttggaga ttgtccgtcg gtcagaaggt ggctgtccaa gggcaggcgc
     3961 aggcggctgt ctaaaacgat gcgtgcgggt tggcgcaaag ttggaaaagc gcggacgttg
     4021 agccggggat tgtccgccaa caccgtgccg atgccggtca gcaccgcgca gctttcggca
     4081 cgcaaaacct gtacgtcggc acgcgcgtct tcgccggtaa tccaaaagct gctgccgtct
     4141 gaaagggcgg ttttgccgtc cagcgaaacg gcgcatttga ggcggacaaa ggggcggcgg
     4201 cgttcgatgc gcgacaggaa gcctcggttg agttcccttg cctgatgttc gagtaaaccg
     4261 cattccgtct tgatgcctgc tgcttcgagc atggcaaacc ctttgcctgc aaccggcggg
     4321 ttggggtcgc gcatggcggc aacgacgcgt gttacgccgg aacggagcag agcttcggcg
     4381 cagggcggtg tgcgcccgta atggctgcac ggttcgaggg taacaaaggc ggtcgcgcct
     4441 tgagccattt cgcccgcctg atgcagggcg tggacttcgg cgtgcggttc gcccgctttg
     4501 acgtggaagc cctgcccgac aacttggctg ccgtgtgcga taacgcagcc gacgcgcgga
     4561 ttgggcgaag tggaaaaacg ccccaaagcg gcaagtcgga gggcgttttc catcatggat
     4621 atatctgtgt ccgaaaacat ggggataccg tatcagtatg ggttggggga atcaggcttt
     4681 gccgcctgtt ttgacggctt gcgccagcca cgaggcaaag tctgccggat tgtcgaagcg
     4741 tttgtgcagg gcggcgaaac ggacggcggc ttccgtgttg tgctcaagca gctcttttag
     4801 caccatgtcg gcaagtgcgg cagaggggat gtcgcgctga cccgaagtgt agagcctgtg
     4861 ttccgtcagg cggacggttt cgtcgatctg ttcgggtgtc agggcggatt ttcgggcggc
     4921 ggcggtcagg tcgttgcgga ggcgttgtgc attgaagggc gaacgttttt tgtccggacc
     4981 gatgacggcg ggcattttga gttcggcggt ttcgagcgtg ccgaagcgtt tgccgcagtt
     5041 ggggcagtgg cggcggcggc gcacggcgtt gcgttcttcc atcagacgcg aatcggcaac
     5101 gcgggtgtcg gggtgggcgc aaaacgggca tttcatggcg tttcttcctg atgccgtctg
     5161 aacgtcaaac cgatacgccg gcggcgcggg cgatttccag accttcttcg gcactcatat
     5221 agacggggtt ttcgggacgg tcgtgtcgga cgatgttgcc ttcgcggaac atgaccagtt
     5281 cgtccacggc aagttgggac catgattcgt cgcgggtcag cggcagggtg gcgataacgg
     5341 cgacgcggtc ggagggcgtg gttacttcgg caaaatcgac catcacgtcg tcgtcgagca
     5401 ggcgcgcctt gccgaacggg gcttggcgga cgatgtagtg cagtagtgtg ctggcgtggg
     5461 caaacagggc aatgccgtct gaaagcataa agttaaacag cccgaacttg cggatttcgt
     5521 gcgtcagccc cgcaatcgcg tcaaacagcg tgtcgtcgtc gggacgggcg gcaaagcggg
     5581 tgcgcaggcg gttaaggatg tggcagaacg cgcgttcgga atcggttgtg ccgacggggt
     5641 ggaaaaattc gccctgttcg ggaaaaaaat caatcaaatg tccgttgtgg gcaaacagcc
     5701 agtagccgcc ccacatttcg cgcataaagg gatgggtgtt cgccagcgag gtttgtcctt
     5761 gtgatgcttt gcggatatgg gcgacgacgt tttccgattt aatctggtag gcgcgcacga
     5821 ggtcggcgac gggcgaattg gcactgggtt tgtcgtcatg gaacaggcgc acgcctttgc
     5881 cttcgaaaaa gccgatgccg aaaccgtcgg catggtggtc ggtaatgccg cccctgcggc
     5941 ggaagccttc aaaggaaaac ataatatcgg tcggcgtatt gcagttcatg cccagcagtt
     6001 gacacatggt ttgtcccaat gattcagatg gtcgcaagta ttcggattat accccgaact
     6061 gaaaatgccg tctgaaatac ggcttgttcc ccgttattcc ctcgaaaaca gaaaacagaa
     6121 aacaaagacg gaaacttaag attccgtcat tcccgcgcag gcgggaatcc gacttgtccg
     6181 gttttggttg tttttcgttc cgtaactttt
//
LOCUS       DAWKQB010000119         6206 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.119, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000119 DAWKQB010000000
VERSION     DAWKQB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.119"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            120..1673
                     /locus_tag="WJL14_001665"
     CDS             120..1673
                     /locus_tag="WJL14_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ7656712.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1764..3791)
                     /locus_tag="WJL14_001666"
     CDS             complement(1764..3791)
                     /locus_tag="WJL14_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ7656713.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEIGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLGLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGVLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4080..6086
                     /locus_tag="WJL14_001667"
     CDS             4080..6086
                     /locus_tag="WJL14_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ7656714.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDVHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1591 a         1889 c         1446 g         1280 t
ORIGIN      
        1 cgtatgctac cgcatactgg cctttttctg ttagggaaag ttgcacttca aatgcgaatc
       61 cgccggcata ttgttttcaa atgagggcgt tctccgtccg caaccataaa ggaagtttca
      121 tgaaccggac ttatgccaat ttctacgaaa tgctgaccgc cgcctgccgc aaaaacggca
      181 acggcacggc agtgttcgac ggcaaggaaa aaaccgccta ccgcgcgctc aagcaggaag
      241 ccgaagccgt tgcggcgtat ctgcaaaata tcggcgtgaa gttcggcgac acggtcgcgc
      301 tggcggtttc caattccacg gaatttatta ccgcctattt cgccgtatcc gccatcggcg
      361 cggttgccgt accgatgaac acatttttga aaaacagcga atacgcgtat atcctgaacg
      421 actgcaaggc gcgcttcctg ttcgcctcgg ccggcctgtc aaaagaattg gcgggcttga
      481 aggcgcaaac gcccgtcgaa aaaatcattt ggacgggcca aagccgtccg gacggcgaaa
      541 tggcggaagg cgatgccttt tttgaagacg tgcgccgctt ccccgaaaaa cccgacttgg
      601 gccgccaacc ccggataaat gatttggcac acatcatcta cacctccggc acgacggggc
      661 atcccaaagg tgcgctaatc agctacgcca acctgttcgc caacctgaac ggcatcgaac
      721 gcatctttaa aatctccaag cgcgaccgct ttatcgtttt cctgccgatg ttccacagct
      781 tcacgctgac ggctatggtg ctgctgccga tttatatggc gtgttcgatt attttggtca
      841 aatccgtttt ccccttttcc aacgttttga aacaggcact gctcaaacgc gcgaccgtgt
      901 ttttgggcgt gcccgcgatt tacaccgcga tgagcaagac gaaaatccct tggtatttca
      961 gatggttcaa ccgcatccgc ctgtttatca gcggcggagc acctttggcg gaacaaacca
     1021 tcctcgattt caaagccaag ttcccccgcg ccaaattgct ggaaggctac ggactgagcg
     1081 aagcctcgcc cgtcgtcgcc gtcaatacgc ccgagaggca aaaagcccgc agcgtcggca
     1141 tccccctgcc cggtttggaa gtcaaagccg tcgatgaaga attggtcgaa gtgccgcgcg
     1201 gcgaagtggg cgaactgatc gtcaggggcg gttcggtgat gcggggctac ctcaatatgc
     1261 ctgccgccac cgatgaaacc atcgtcaacg gctggttgaa aacgggcgat ttcgttacca
     1321 tagacgaaga cggctttatc tttatcgtcg accgcaaaaa agatttgatt atttccaaag
     1381 gtcaaaatgt ctatccgcgc gaaatcgaag aagaaatcta caaactcgat gccgtcgaag
     1441 ccgccgccgt catcggcgtg aaagaccgtt atgccgacga ggaaatcgtc gccttcgtcc
     1501 aattgaagga aggtatggat ttgggcgaga acgaaatccg ccgccacctg cgtaccgtgc
     1561 tggcaaattt caaaatcccc aaacaaatcc actttaaaga cgggctgccg cgcaacgcta
     1621 cgggcaaggt attgaaacgg gtgttgaagg agcagtttga cggaaacaaa tgaacgccgt
     1681 gccgtccgaa gccccgtccg gcaaaaaaaa tgcggtgaat ctgcttcacc gcattttttt
     1741 ggcaaacgcc ggagcgagga tatttactct gccaattcca cctgctcgtg cgccatcagt
     1801 tcctgaccga catcatccaa caacatcaaa taacgttcgt agtttttcaa aatgtcggca
     1861 atcagctcgt ccttgttcat atactgcaca tcgtacccga cgcgcccgtc gaaaaaataa
     1921 gcgtagggtt tgtaagttgt ctgatgccgg atatgcggca gcttgccgtc gttaatcaac
     1981 tggtcggata catcctgccc gacagactta atcccgtaca taaaatcgcg catcgtctct
     2041 ttccgaatga cgaactcgat tgcgggctcg tcctgatgaa acatcttatc gacccggacg
     2101 ctcaagccgt attcttccga aagctcccgt tgtaactcgt gcatagcggg cgatgcggta
     2161 tgtttgagga attttaaaat atcctgctcc tgcgtctggc tcattatctg caccagccgt
     2221 tctttccact tgccgcccgt ccaaaataca ctggtagggt taacccgggt ctcaaaatat
     2281 ttcttatccg cactcaatcc tttccacagg ctgaaacaca ttatcagcat cagcagggca
     2341 aacggcaggg aaacaatcag ggtcatagac tgcaggttgc cgagtccgcc cgagcgcatc
     2401 agcaaaacgg caacggcaga catcagcacg ccccacataa ccgcctgcca ccgtggcgcg
     2461 ctcaagcctt tgtcccgaga ggtaatattg ttcaggacat aaatcccgga atcggcagaa
     2521 gttacaaaaa acagagaaat gaccagcagg ctgacgatgc tcgtcaattc gggcaggggg
     2581 aggtaattaa agaatttaaa aagcagcgtt tccggagagg aggtcatctt ttcgagcact
     2641 ccccccgcaa ccccgtcatt cagccaaatc gccgtattgc cgaagacggt aaaccacaaa
     2701 acgccgaaca ggccggggat gagcaaaacc ccgaagacaa actcgcggat ggtgcgcccc
     2761 tttgaaatgc gcgcgataaa caaacccaca aacggcgccc aagaacacca ccacgcccaa
     2821 taaagcaccg tccaagattc aaaccacggc ttgtgttccc gttcgtacgc ataagtttta
     2881 aaactgaggc gcaccagatt tccgaggtag ttccctatgt tgtcgccgaa tgccgacaac
     2941 aggtaaacag tgggacccgc cgccaaaaca aaaaacagca gcaaaaacgc aagacccagg
     3001 ttcaactcgc tcaacacctt cacacccttc cccacgccgg atattgccga aacgacggcg
     3061 agggacatga cggcggcgat aatcaaaacc tgcacgctga agctgttttc ggcaatccag
     3121 cctatttcct gcaatccggc gcccagttgc gaagccccga accccaatgt ggtgatgatg
     3181 ccgaaaaaag tagcaagcaa cgccataata tcaatggcat cgccgaacct tccggaaatt
     3241 ttttctttca acagggggta aaaacaagaa cgcagggcaa gcggcagctt gtagcggaaa
     3301 ccgaaataag ccaaagccaa tgcaatcgta ccgtacaccg accaagcgtg aacgccccaa
     3361 tggaacaccg tgtgcagcaa tgcctgctgc tgcctgtgtt ccggcgtgcc ggccgtaatg
     3421 tccgaaaaat aatgcatcaa cggctctgcc acgccgaaaa acatcagacc cacgcccatc
     3481 ccggccgcaa acagcatcgc cagccacgac aggaagccga attccggcac atcttcatcc
     3541 cgtccgagcc tgatgtttcc caaactgctg accgagagta tcagcaggaa acccagaaaa
     3601 atggaaaacg ttaaaacata aaaccagctg aactcggtaa aaatgacttc ttttgcccga
     3661 tcgagccaca tctgcacctg atccggcacg gttaaaacca ataccaccaa aacacacaca
     3721 aaaaacaaag tcgtcaaaat aaccatcgga ttaaatgacg ttcggcgttc tataaattca
     3781 gacagggaca aaccttctca ctcctttgtt aaaaacagac aaacccggtc atcgggcaaa
     3841 gcggtcaaaa cctgccggta aaataccggc ttccggatga cgaaatgcac aaaccggccg
     3901 aaattgttat aatcgaaaaa attaaaaatc aatacggatt attctatcag attgatttat
     3961 cgatatttat tatatcatta atatagtttg atttcaaacc ggcggaaaat gcgccgccca
     4021 ccgcaggcgc gccctccccg ctatcggggc gtttaacgcc ggattgccga tgcggtacaa
     4081 tggctgatat gaagaaaatt acccctcaaa acctgcgccc cctgctttcg gaaagcttgg
     4141 gacataccga ttttgtcaac gtcctcaacg cactgattaa atttttgcgc cgtggcggca
     4201 aaaaatgtgc gggggaacgt ttcgacctga ttatcgacac attcaaacaa gacagggaat
     4261 tactgtcccg cttcagccgg tgtttttaca tctggctcgc gcaaatacac atttatccgg
     4321 cactcatcaa actcggcatc ttctcgcgcc acagctttgc ccgggaaatg ggcatacgca
     4381 tctacgaacg cttcagcccg tcatataaag attttgccaa cttgggcgaa gtcttccttt
     4441 atcttttcca ttccgaaaac gacgacaaat ggctgcaaac gctcaatatc cgccaatggc
     4501 tggttttata cgaactcatc cggagccacg ccgagccgtc caaattgcag acggcgggca
     4561 tccgccttgc cgatgcgcgt ttgcgcgcca tcgaaatgct gtctgtctgg acggcatccg
     4621 aagccatcga acccgacctc atccgcatcg ccccgcgcct gctggaagcc gattcttcct
     4681 tcgtcgccct ccaacgcgaa accgccaaac tggtcgaaca ctaccgcaac ggcaccacgc
     4741 cttacgacac cgcccacctc gaagtgatgt tcgaccaatg tttcagccag attgactatt
     4801 tgcgccgcaa agggacgggc gccggctccg gttcgtcggt caaagtcgcc cacctgctcg
     4861 aacggctccg gcagaccgta gaccgtctaa agctgctcac cgacatccaa accggtgccg
     4921 gcaacagcaa ccgcctgacc atcgcgctga tgaactccct catctacgcg gcggtcgaac
     4981 aatacagcac ccgccacctg cgccgcagca gcatccgtat gctcgcccgc agcattaccg
     5041 aaaacaaaag ccaccacggc gaacactaca tcacccgcaa ccgcaaagaa tatttcaaaa
     5101 tgttctactc ggcggcaggc ggcggcatca tcatcgccct gatggcgctg ctcaaaatcc
     5161 gcatcggcac actcggcctc agccccttcc tgacttcctt gtcggctggg ttcaactacg
     5221 gcatcggctt tatgatcatc catatgctgc actgcaccgt cgccaccaaa cagcccgcga
     5281 tgactgccgc cagctttgcc gaacaggtcg acctcaacga aggcggcaaa gcggtggaca
     5341 acaaactcgc caagctcctc atcgacgtat gccgctccca aagtgtcgcc gtcttcggca
     5401 acgtttccat cgccatcctt ttggcgtgcg ccatatcgtt cggctatgcc catctgcacc
     5461 aacagcccat actcgatgtc cacaccgccg cctaccagtt caaatccata gacatcatcg
     5521 cttacccgac gctgtggtat gccgccattg caggtctgtg gctgttctgc tccggcatca
     5581 tcgcaggctt cttcgacaac cgcgccgact acctcaacct gcgccaacgc ctgcccttca
     5641 accccttgct gcgtaaaata atgcgccccg gaccccgccg cgtcctcgcc gcctacatcc
     5701 acaaacacta cggctcactg gcaggcaact tcatcttcgg gatgctcttg ggtatgaccg
     5761 gctatttcgg acacctcctc gggctgccgc tggacatccg ccacgtcgcc ttttcctccg
     5821 ccaacctcgg ctatgccgcc gtcagcggca acgtcggttt gggcacgttc gtactcggca
     5881 ttttcagcgt cctcgccatc ggcctggtca acctctgcgt cagcttcagc ctcgccctct
     5941 tcgtcgccct gcgctcgcgc ggcacgaaaa tcggcagcat ccgcaatctg attaagagtt
     6001 tttggaatca gattaaaagc aatccctgca tacttttcct cccgcccgcc aaagaacagg
     6061 gacatcctcc ttcggacaag ccttgaccgg caatgccgtc tgaagcggga ttcgccccga
     6121 ataccgccct gatgcgggaa atccccataa aaggatgcaa aaatgccgtc cgaaccgaaa
     6181 cgtggttcag acggcatttt aaaagg
//
LOCUS       DAWKQB010000120         6118 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.120, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000120 DAWKQB010000000
VERSION     DAWKQB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.120"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WJL14_001668"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WJL14_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ7656715.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGVPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDIVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="WJL14_001669"
     CDS             complement(2288..2560)
                     /locus_tag="WJL14_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656716.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="WJL14_001670"
     CDS             2746..3432
                     /locus_tag="WJL14_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656717.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="WJL14_001671"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="WJL14_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ7656718.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="WJL14_001672"
     CDS             3919..4065
                     /locus_tag="WJL14_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656719.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..4526
                     /locus_tag="WJL14_001673"
     CDS             4062..4526
                     /locus_tag="WJL14_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ7656720.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPELPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="WJL14_001674"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="WJL14_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ7656721.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFHNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1384 a         1356 c         1627 g         1751 t
ORIGIN      
        1 aaaaaatttc cagagaaccg atacaacagt tggaacttgg gcttgggaat attacggtag
       61 ataaacttgg aaactctgtt atgctatggt cttttgtcgc aattgaaaaa aacgcgaacc
      121 gaatggttcg cgttttttca gacggcatta attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgatgtca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgcgcgt aaaccatttc ccccagtttt tggctggctg
      301 tgcccagcgc ttcggctttg gcatcgatag ctgctttgtc gtcgccttta actgcttctt
      361 cagcttcttt caacgcggct tcgatttttt ctttctcggc tgcatcgagt ttgtcgccgt
      421 agtcggccaa agattttttc acggagtgaa tcagggcttc ggcttggttg cgggaagcga
      481 ccaattcagt cagcttttta tcttcctcag cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt gatgttggct gctttgcctg
      601 tgcctttgtc tttggcagaa acgtgcagga taccgttggc gtcgatgtcg aaggtgactt
      661 cgatttgcgg cacaccgcga ggtgcgggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc ggaggcgcgt tcgcgttcac cttgcagtac gtggatggtt actgcacttt
      781 ggttgtcttc ggcagtagag aacacttgag acgctttagt cggaatagtc gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc tcaatacttc gccttggatc gctgcgccta
      961 cggcaacggc ttcatcaggg ttcacgtctt tgcgcggttc tttgccgaag aagtctctca
     1021 cggcttcttg tactttcggc atacgggact gcccgccgac taaaattacg tcgtcgatgt
     1081 cgccggtgct caagccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcattgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttgatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcagacgttg cagagccatg acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttgaact cgtcaatgat gtagtcgatc agacgttggt
     1381 cgaagtcttc accgcccaag aaagtatcgc cgttggtagc caatacttca aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgttttacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tggcttgacg ttggctgtcg ttgaagtagg ccggaacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgcgctt tcacccacgc gtcgccgttg ttggctttga
     1801 tgatttcaaa aggcatggat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaaacg tttggcggcg taaatagtgt ttttggcgtt ggttaccgct tggcgtttgg
     1921 caggcgcgcc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcatttaa gcataatatt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaatttttc gcacagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac cttgggcaat
     3001 gaagttgata cggacgagtt tgtctttgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg cgatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tctatggagg gggttttgaa tgacggcgat tcgattttgg tcaatcatgg tgaaaatacg
     3241 ccgagggacg gtctgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggattat caatgtgatt tctgcaaacg aggcttatcc tgcttttgaa
     3361 atcaatttga acgatttgac cgatgatgtg gagattatcg ggcgtgtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaattg caggcggtca aacttatcta ttggaacaat
     3481 tcctttttca aaggcgaagc ctgcttgcct ttaaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaatcac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gacgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggt gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttagaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcttaccc gccccaagtg gatcgcgggc
     4141 ttatccggga gtgccttaag gagaatctcg gcgaggaaaa ggcggaagga ttgatcgaat
     4201 cgctcgattc caaacctcaa gtgctggttg aggaaaaatg cacttgggcg aaacgggaag
     4261 agttgcatga ttatttcagc tatttgggtt tggatattat tacccggaga tatatggagt
     4321 tagaaacggt cgtgccgccg gaggaaggtg agggcgaagg agagggcgcg gatggggaaa
     4381 tgcccgaata tcttgaactt catggcgggc gggaagatga tatttccgca ccttcgcaac
     4441 ccgaactgcc gacccgcaat atcaaaccgc tgcttttcgg gctgctgatt gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggtcatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgtg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gccgtccgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gttttgtcca cacgccccga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgttg ggtcagtcca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccatcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac gagtggcggt taaagttttg cgccccaacc ttttgcccct tatcgaacag
     5041 gatttgtcgc tgatgcgttt cggcgcggcc tgggtcgaac gcttgttttc agacggcagg
     5101 cgtctgaagc cgctcgaagt ggtggcggag ttcgacaaat acctgcacga cgagttggat
     5161 ttgatgcgcg aagccgccaa tgccggacag ctcgggcgca atttccacaa cagcaatatg
     5221 ctgattgtgc ccaaggtgtt ttacgactac tgcaccagcg acgtgctgac gattgaatgg
     5281 atggacggta cgccggttgc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggctttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgacaaac gttatctcgc catcaatttc
     5521 ctcgcctttt tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggtcggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcattcctt cattgccgcg caaaatcagt
     5941 gcgttgattg atgaaaaccg ccagcaggaa atgcgtgatg cctatatcca tttggtcaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaatcatcg cccgactccg cccgtttata aggaaatc
//
LOCUS       DAWKQB010000121         6051 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.121, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000121 DAWKQB010000000
VERSION     DAWKQB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.121"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(51..1085)
                     /gene="purM"
                     /locus_tag="WJL14_001675"
     CDS             complement(51..1085)
                     /gene="purM"
                     /locus_tag="WJL14_001675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ7656722.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGCIRER
                     QGDEHQTQVA"
     gene            complement(1267..1464)
                     /locus_tag="WJL14_001676"
     CDS             complement(1267..1464)
                     /locus_tag="WJL14_001676"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656723.1"
                     /translation="MFRTESEAGASVPQSDFSGPDAGVSTGKKKILILMMASKNSSAR
                     PPKEMVKKSNASFDGTQYIRN"
     gene            complement(1606..1690)
                     /locus_tag="WJL14_001677"
     tRNA            complement(1606..1690)
                     /locus_tag="WJL14_001677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1654..1656),aa:Leu,seq:tag)
     gene            1838..2515
                     /locus_tag="WJL14_001678"
     CDS             1838..2515
                     /locus_tag="WJL14_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656724.1"
                     /translation="MPAALIKDFLLTQGLKLPPDEVRTAYLTAKTVMDMGMASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRSTDVRSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            2508..3101
                     /gene="ribA"
                     /locus_tag="WJL14_001679"
     CDS             2508..3101
                     /gene="ribA"
                     /locus_tag="WJL14_001679"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ7656725.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            3257..4270
                     /locus_tag="WJL14_001680"
     CDS             3257..4270
                     /locus_tag="WJL14_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656726.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     RGGILFDLQQLADKKVDLILHPSSFNYRDIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            4492..5829
                     /gene="ribB"
                     /locus_tag="WJL14_001681"
     CDS             4492..5829
                     /gene="ribB"
                     /locus_tag="WJL14_001681"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ7656727.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKVGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
BASE COUNT         1518 a         1591 c         1495 g         1447 t
ORIGIN      
        1 aaaatgccgt ccaaacccat gtttgagacg gcatttcgct atagaagcaa tcaggcaacc
       61 tgggtttgat gctcgtctcc ctgacgctca cggatgcaac ctaaacggta aactgtttca
      121 ccttgttcac ccaagagacc ctgaaccgca tcggcatctt cggcagcaac aataacgacc
      181 atgccgatgc cgcagttaaa ggttcggtac atttcttggg tttccacatt gcccgccttt
      241 tgaagccatt ggaagagctt gggcaattcc cacgatttag catcgatttg tgcaaccgtg
      301 ttttcaggca acacgcgcgg cacgttttcg gtaatgccgc cgccggtaat gtgtgccata
      361 cctttaatgg taaatttttc caaagcggca agaatcggtt tcacatacag acgggtcggc
      421 gcaataacag cctcacgcaa ggttttgcca ttatcaaact cggcatccaa atcgggattg
      481 tcgcgttcga tgattttacg gataagggaa tagccgtttg aatgtgcgcc gttggaagcc
      541 aaacccaata ccacatcgcc cgcgccgatg ctgcggccgg taatgacatt ctctttttcc
      601 actacgccga cggcaaaacc cgccaaatcg tattctccga cgggatacat acccggcatt
      661 tcggcagttt ccccgccaat cagggcgcag ccggattctt cgcaaccttg tgcaatgcct
      721 ttgatgacat cggttgcgcg cggaacatcc aattcgccgc aggcgaaata atccaagaaa
      781 aacagaggtt ctgctccttg aaccaaaata tcgttgacac tcattgcaac aaggtcgatg
      841 cccaccgtat cgtgtttatc ccaatcaaag gcaagtttga gcttggtacc cacgccgtcc
      901 gtaccgctga ccaatacggg atttttatat ttcttgccga tttcgaccaa tgcgccaaaa
      961 ccgcccaaat cccccaatac ttccggacgc atggtgcgtt tggcaaacgg tttgatgttt
     1021 tcgaccagtt ggtcgcctgc gtcgatatcg acacctgcat cgcggtaact caatgaagta
     1081 ctcatcgttt ttccttggta aatggggatt ggacggtaaa ataacggggc gtattctacc
     1141 ttatttcacg tttgcaggtt cagattttta gacaatattg taaacagtcc gccatatgcc
     1201 cgcgcgtgtc gggtttggcg ggaccgtccg caggattaac gggcagaaac ccgcctgccc
     1261 tttccctcaa ttccttatat attgcgttcc atcaaaagac gcattacttt tcttaaccat
     1321 ttcttttggc ggacgggcag aagagttttt tgatgccatc atcaaaatca atattttctt
     1381 ctttccggtt gaaaccccgg catccgggcc ggaaaaatct gattgcggga cggaagcacc
     1441 cgcttccgat tcggtgcgga acaaatggcg gtgcttttat gtgccgtttt atgtgttgaa
     1501 acacataggc agataaaaag ccgcccgctg aaaagcagac ggcttatatt ttgtgccacc
     1561 gaattgtccc aataagcatt aacaatatga tttatatatt tttattggtg cggacggaga
     1621 gactcgaact ctcacacctc tcggcgccag aacctaaatc tggtgcgtct accaatttcg
     1681 ccacatccgc ataagggtgg agattataca gattttgtcc gattgcgcaa ggctttgggc
     1741 gtaatagttg aggcttatgc tttgcagcgg taaaatccgc tattcatccg ctcggcatcg
     1801 gaatccggcg gttttatttt attgacggaa tttgagtatg cctgctgctt tgattaaaga
     1861 ttttctgctg acccaaggtt taaagttgcc gccagacgag gtacggacgg cgtatctgac
     1921 cgcaaagacg gtgatggata tggggatggc ttcgattgag cgttcggttt tgtggcttga
     1981 gcatgaggat tggaggcttg ccgattattt ggagcaaaac agcgggaatg aggaaggtct
     2041 gaaacggctt ttcatggctt tggattcggt gttttcacgc tcgacagacg tgcggagtgc
     2101 ggcggtttat gccttgatgc catctgaaaa ccaggctttc caactgatat gcctgtcccg
     2161 acagggcgag gttttggaaa acctgtggga tttggatgaa gcggcaggca aggtttcgct
     2221 ggcttgccgt tcggcgcaaa gcggttggat gaatgttgcc tcggatgtac gccgttggtt
     2281 ggatttgggg gagctttcgg gagaacgcaa tcatgcttcg gcggcgcaaa tttccattcc
     2341 ggtctgcacg gaaagtggcg gtgtgttggg cgtggttcat gtggaatttg aatgcgcaga
     2401 gtgtgcgggt acggcagcac aggtggaatg ggtggctctt gccttggctt tgtccgaacc
     2461 tttgaaactg ctgttgggta tgtctgccgg aaaagatggg agtgaagatg tctgaaatgt
     2521 tgaaccatgt ggcatcctgc cgcctgccga ccgaatgggg cgtatttacg atgcacggct
     2581 ttgaagaggc aaacgggcag gaacacgtcg cgctgaccgt cggtaatttt tcagacggca
     2641 atccggttct gacgcgcatc cactccgaat gcctgacggg cgatgcgctg ttctcgagaa
     2701 aatgcgactg cggacctcaa cttgaagcgg ccatgagggc ggtacagaca gaggggcgcg
     2761 gcatcatcgt ctatctgcgt caggaaggac gcggcatcgg gctgattaac aaaatccgcg
     2821 cctatcatct gcaagaccaa ggtatggata cggttgaagc caatttggca ctcgggctgc
     2881 ccgtcgatgc ccgcgatttc cgtttggcgc aatctatcta cgaatatctg ggcatccgct
     2941 cggtcaaact gctgaccaac aaccccgaaa aaatccaaac cctgaaagat gcggggatta
     3001 acgtggtcga acgcatcccc ctgcacgtcg gggaaaacct tgaaaacaaa cgctacctcc
     3061 aaaccaaagc agacaagctg ggacatctga tgtcggaata aggcaaagtt gcagggaacg
     3121 ggcatcctgc gccgcctttc gggaaacagg tttccatacc ttgataaagc aataagtttt
     3181 atagtggatg caaacgcatt gcgagcgcgg gtttgaggca tacgcgcaaa catcttaata
     3241 taatggattg atatttatga ttttctccat catcgtccct atttacaatg tggaaaaata
     3301 cctccgctgc tgcgtggatt ccgtgcttgc cgaaaatttt gccgattatg aaatgatttt
     3361 ggtcgatgac ggttcgccgg acggctgcgg gaagatttgc gacgaatatg caggcaaata
     3421 tccgcatata aaagtgattc atcaagaaaa cggcgggctg tcggatgccc gcaacgccgg
     3481 tatccgggcg gcaaaaggcg attacctaat ctttttggac agcgacgact attgggccga
     3541 caccaatcgt tcaaaaaacg cgcggggggg gattctcttt gatttacaac aacttgcaga
     3601 caaaaaggtt gatttgatcc tgcatccctc gtccttcaat taccgcgaca tccccaaagg
     3661 ggcggacttt tcggataatg attttgtccg ccattttgaa acgctggtgg aggggcggta
     3721 ctatatcgcc aacgcgtgga caaaaatcgt caggcgggaa ataatcatca aaaacaatct
     3781 gtttttccca aaaggataca ttcacgagga tttcccgtac agtttgcaat tggcgcgttt
     3841 tatcaagact tttgcctttt acgataaccc tttttaccag taccgcgttc tcggcggctc
     3901 tatcagccac aacatcaaat acaaaaattt cagcgatgtg ctgacgcatc tcgaccgggg
     3961 tgtggatttt ttagtcgaaa acaaaaattc ccccatctac ggcggtttgc aaaaatttgt
     4021 cttcgacaat atcggctatc tgaggtctat attggtaagg ctttattttt ccaaaaacat
     4081 tatcctcatc taccggaaat atttttcatt taaagaaaaa tgcagaaaga tattcggcgc
     4141 gaaggcaatc cgtccggttt ttatcgggaa gaccgcattc atcataggat tgccgatatt
     4201 gcgcctgctc gtaccgccta tgctgtaccc ggcaatcaag gccgtttatc agaaattttt
     4261 ttcggaataa gcttccggca aaccccaaat cagaagtgcg cgggaagttg ggacaaacag
     4321 cccccggccg gcaaggattg cggcaatgcc gtctgaagcc acgaatccgg cttcagacgg
     4381 catttgttta ccaaaaagca aataattcgg tttggcgaaa aaaacagatt tgctttttgg
     4441 taaatacgcg attacaatcc gctacatccg atttctacaa aggatgaaac gatgaccgac
     4501 acagccggtc tgcgccgccg caacctgcgg caatggatag cagaacacta cggcggtttg
     4561 caaacccgtt ttgccgaagc cgttgccctc aacacaggcg aactctccgc ccttttgaaa
     4621 aacaaatcct tcggcgagaa aaaagcccgt aaaatcgaac aggcggcaaa aatgcccgcc
     4681 ttctggctcg ataccgaaca caccgcccgc ccgcccgaac acacaggaaa acacaccatg
     4741 tcccatattt cccccatccc cgaaatccta gccgacatca aagtcggcaa aatggtcatc
     4801 atcaccgatg ccgaagaccg agaaaacgaa ggcgacctgc tgatggcggc gcaattcgtc
     4861 acgcccgaag ccatcaactt catgatcaaa cacgcgcgcg gcttggtctg cctgccgatg
     4921 gacggcgaaa tggtcgaaaa actcgggctg ccgatgatga cccaaaaaaa cggcgcgcaa
     4981 tacggcacca actttaccgt ctccatcgaa gccgcacacg gcattaccac cggcatttcc
     5041 gccgccgacc gcgccctgac tattcaaacc gccgtttccc cgaccgccaa acccgaagac
     5101 atcgtccaac ccggtcatat ctttccgctt cgcgcccaaa aaggcggcgt actcgtccgc
     5161 gccggacaca ccgaagccgg cgtcgacctg gcgcaaatga acgggctgat tcctgccgcc
     5221 gttatttgcg aaatcatcaa cgacgacggc acgatggcgc gtatgcccga actgatgaaa
     5281 ttcgccgaag aacacaagct caaaatcggc acgattgccg acctcatcga ataccgcagc
     5341 cgtaccgaaa gcctgcttga agacatgggc aatgcgcctg tacaaacccc gtggggcgag
     5401 ttccaacaac acgtttacgt cgacaaactc tccggcgaaa cccacctcgc cctcgtcaaa
     5461 ggcacgccct ccgccgacac cgaaaccctc gtccgcgtcc acgaaccctt cagcgtcatg
     5521 gacttcatcc aagctaaccc gcgtcattca tggtcgctgc ccaaagcact cgagcgcatc
     5581 caacaggccg aaagcggcgt agtcatcctt ctgcaccgca ccgaagacgg cgcatccctg
     5641 ctcgaccgca ccctgcccaa aggtgcaaac caagcctaca aatgggacag caaaagctac
     5701 ggcatcggcg cgcaaatcct cgccggcctc aacgtcaaaa aactgcgcgt cctcgggcag
     5761 ccctcatctt tcaccggcct gaccggcttc ggtttggaag tcgtcggctt tgaagaagcg
     5821 gaaaaataat atagtaaatt caaatacttt atatttgctt tatttattgc attatttccg
     5881 tgcaaacgaa aacccggtct gttgggttgg attttgtttt tgcaaatttc gggtaacttc
     5941 taattcgtca ttcccgcgca ggcgggaatc cggttcgtcg ggtttttgtc atttccgata
     6001 aattcctgtg gctttggttt tttggattct ctctttcagg gaaagaacgg c
//
LOCUS       DAWKQB010000122         6040 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.122, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000122 DAWKQB010000000
VERSION     DAWKQB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.122"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJL14_001682"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="WJL14_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ7656728.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWL
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYEAAVQSAFQDVANALTAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJL14_001683"
     CDS             complement(1515..4718)
                     /gene="mtrD"
                     /locus_tag="WJL14_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ7656729.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMADEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJL14_001684"
     CDS             complement(4730..5968)
                     /gene="mtrC"
                     /locus_tag="WJL14_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ7656730.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQADVQTASEAKTASEAE"
BASE COUNT         1366 a         1704 c         1617 g         1353 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcacctt ccgccgaata gctgctgcgt
      181 tccgcatcga gcaagtcgag cgcgccggat acgccgtgtt tgtaacgcag accgaccaaa
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccaac
      301 tgctcgcgcg cggtcaatgc gtttgccacg tcttgaaatg cggattggac ggcggcttca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat cgaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatggaa ggtgcgaaca accaaacgcc ggtgccgctt
      481 ttgaacagcc cgcccaattc ggcagaatgc gtatcgacgc ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagcttc
      661 tcaacaaaaa actgcttgtc caacggcaaa ccggcgggca ggtcgtcggg tatcggttgg
      721 ttaatcaggg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat tagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcataa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatac ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacccaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaaagtcc tgatgccgtc tgaaaacccg tgggcgttca
     1501 gacggcatgg ttgcttaatg ttggctgtcg tccgaaccgg taatgcccgc ttcggcggcg
     1561 tgttttactg ccatttcgtg ttcgtgcgcg gtttctttga agaatttgcg caccaccaca
     1621 tagaaaagcg gaacaaggaa cacggacaag agcgtgccga tgagcatccc ccagaatacg
     1681 gttgtaccaa tggcgcgctg gctggcagaa cttgcaccgc cggcaatata caggggaacc
     1741 acgcccaaaa taaaggcgaa cgaggtcatg ataatcggac ggaaacgcag gcgggctgct
     1801 tccaaagcgg cttcaaccgc gcttttccct tgcgcttgaa ggtctttggc aaactcgata
     1861 atcaaaatcg cgtttttcgc actcaaaccc atcacggtaa cgaaaccgac ttgaaagtag
     1921 atgtcgttgg cgaacgaggg aacgctgccc aacagccctt caaacaggtt gcgcccggtt
     1981 acgcccgcag ccgcaccgat caaacccaac ggaatcacaa ggatgaccgc cagcggaatc
     2041 gaccagcttt cataaagcgc ggcaagtacc aaaaatacgg ctgcaaccgc caaaccgtac
     2101 aaaatcaggg tttgcgagcc gccttttgcc tcttcgcgcg actgtccgcc ccactccagg
     2161 ctgtaaccgc cgcccaattc gtcaaccatt ttttgaaccg ccgccatagc ctgcccggtg
     2221 gaaacgccgg ttgcaggcga agcggacagc ttcatcgaag gataaccgtt gaagcgtacg
     2281 ctctgttccg taccgttttc ccaagaaaca gtagcaatgg tggaaagcgg tacggcgacg
     2341 ccggatttgt tcggcacggt caggttcaaa atatcggcag gctgcatacg ggcatcctcg
     2401 tcggcctgca ccatcacgcg ttgcagacgg ccttggttcg ggaagtcgct gacataagac
     2461 gaactcagcg cgcttgccaa tgcggtgcgg atgtcggcaa acgaaatgcc ttgcgccgcc
     2521 gcggcggcac ggttgatgtc gattttcaac tgcggcgagt cttccaaacc gccagcacgg
     2581 acggtgctgg ggtcaaacaa accgctggca cgcattttct gaatcaactc gttgcgcttc
     2641 gccagcaatg cggtatggcc ggtattattg cggtcttgca ggttgatgct caggcccgaa
     2701 ccattgccca actccagaat cggaggcggg acgacggcga tgccaaaacc gtctttaagc
     2761 gtccccatca tcatacccgt cagcttgccg gcaatcgcaa cggcatcgct gccgggcgcg
     2821 gtacgctcgt tccaatcttt caatatggca aaacccatcg ccatattctg accgctgccc
     2881 gaaaagctga agccggaaac ggtaatgatg ttttctattt caggaatgct tttcgccagt
     2941 tgggtaactt gcgccaaagt cgcattggtg cgctcttggg tcgctcctgc aggcagttgc
     3001 acgctgacca tgacgaagcc ttggtcttcg gtcggcagga atgaagtcgg caggcgcata
     3061 aacaggaaca cgcccacaac cgccaagccg atatagacaa ccatcatgcg gaaagtctta
     3121 cgcagcactt tggcaacccg gccttcgtaa ccgtgcgtcc aactattgaa tttcttgtta
     3181 aaccagccga agaaaccttt tttctcttcg tgatgccctt tcgggattgt cttcaacatt
     3241 gtggcacaca aagcaggggt aagggtcagc gcaaggaagg cggagaatgc gattgatgac
     3301 gccatcgtca gggcaaactg tttgtaaata ttgcccgtcg ccccgctgaa catcgccaac
     3361 ggtacgaaca cggaaatcag aacggcggta ataccgatga ccgcacccga aatctgaccc
     3421 atcgcttttt tggtcgcttc tttgggcggc aagccttcat ccgccataat gcgctcgacg
     3481 ttttcaacca ccacaatcgc gtcatcgacc acgatgccga tgaccaaaac catcgcaaac
     3541 atggtcagta cgttaatcga catgcccata taagagatga aggcgaaacc gcccaacagc
     3601 gaaatcggta cgacgatggt cggaatcagc gtataacgga tgttttgcag gaagagatac
     3661 attacgacaa acaccaacac catcgcttcg attaaagtgt gaatcacttt ttcaatcgaa
     3721 atttcgacga atttggaagt atcgtaaggg gttttccagc tcataccctg aggaaagtat
     3781 ttttccaacg tcgccatgcg ttctttaacc gcctttgccg tcgccatcgc attgccgctg
     3841 ttggacagca tcaccgccat accggtggta tttacaccgt tcagacgggt tgaggaagaa
     3901 tagtcttcca tacccagtcc gacccttgcc acatccttca ggtaaacatt agaaccgtcg
     3961 gtattggcgc ggagaatgac gttgccgaat tcttcggccg tacccaactg cccttgcgcc
     4021 gttacggtag ccgtaaccgt ctgtccgcga acggcgggaa gcgaaccgat agaacccgct
     4081 gaaatctgga cgttctgggc ggacagcgcg ctgccaacat cggcaaacga caaattgtag
     4141 ttttgcagtt tcttaggatc aacccaaatc cgcatcgcgc gttgcgcgcc gaacaggcgt
     4201 acctgcccca cgccttcgat acgctgcaac tcgggaacga tattacgctg cgcgtagtcg
     4261 ttcatctctt cggttgactg cacatccgac gaaagcatca caatcatcag gaaattggaa
     4321 cgcgccttgg atacggttac gccgtattgc tggacggttg ccggcagcgt gctcaatact
     4381 tcggaaagct tgttctgcac ttccacctgc gccagattct cgtcggtatc gggcgtaaag
     4441 gtcaggctca cgctgccgct gccgctcgaa tcggcggaag tggacatata atccaaacct
     4501 tccacgccgt tcatattccg ctcgatcacg gaaagcacgc tgtcttccat tacctgcgcg
     4561 gacgcacccg gataagtggc gtgcaaagta atggtcgggg cggcgacgga cggatattgc
     4621 gaaacaggca ggcttttgat gccgaaaata cccgccgcaa taatgaaaat cgaaataacc
     4681 cacgcaaaaa tagggcggtc gataaaaaat ttagccatcg atgccttcct tattccgctt
     4741 cagaagcagt tttggcttca gatgccgtct gaacgtcagc ttgaggcgcg gcggcttggt
     4801 tttcagacgg cgcccattct ttgggcgtta cctttttcgc acccgttata ccggcgatac
     4861 tgatgccttc cacaaccacc ttgtccccgt ccttcagacc cgacgtaaca atccaattcg
     4921 taccctgctg ttgcgcaacc gttacctcgc ggggttccat accgccttgg gcattcacaa
     4981 tcatcacggt atctttcgca ccgcgcgtta ccgcctgctg cggcacaaca aatgcgttat
     5041 ccaccgccac ttggtccatc agcacgcgca catacaggcc gggcatcagg atattctgat
     5101 cgttcggtac ggcggcgcgc agggtaatct gaccggtcga ttcgttgacg gtcggatcgg
     5161 caaacagcag gcggcctttt tcagggtaaa ctgtgccgtc gtcaaatttg atgccgaccg
     5221 caatcacacc atccgccgcc agcagtttgc cttcggctat ctgacggcgc aatttcatca
     5281 cttcggatgc agactgggta acgttcacat acatcggatt ggtttggcgg atggttgcca
     5341 gcacggtcgt atcgcccgca ttcaacagcg taccttcgga aactttggac tgaccgataa
     5401 agccggaaat cggcgcggta atgcgcgaac ggttcagatt aatgccggca gatttgattg
     5461 ccgcctgtgc tgctttgaca cctgcctcgg cagaacgttt cgccgttacc gcagcatcgt
     5521 attcctgccg gctgacggct tcggcggcaa ccaaaggctt gtatcgcgcc aaatccgcat
     5581 ccgctttggc aagcgttgcc tgagccgttg ccagttgcgc gcgcgcgctt tccagatttg
     5641 cttcataagt ggaactgtcg atctgataca gcggctgtcc ggcacggaca taactgcctt
     5701 cttggaacag gcgtttttgg ataatgccgc caacttgcgc acggacatcg gcggtacgca
     5761 gcgattccaa acgtcccggc aactcgacgg tcaacgcaac ggtttgcgga tggacggtta
     5821 cgacaccgac gacgggagcc ggtgcttccc gacccgcagg ctgcccgccc tgcgccgcgt
     5881 ctccgccttt accgcaagac gacagtacca atgcaacggc ggcagccaac gcggccgcac
     5941 gcatcgcctt aaaagcataa aaagccatta tttatcctat ctgtctggtt tgatgtaaag
     6001 ggttttgcca atcaacaggc attcttatag tggattaaca
//
LOCUS       DAWKQB010000123         5991 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.123, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000123 DAWKQB010000000
VERSION     DAWKQB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.123"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            61..507
                     /gene="yciA"
                     /locus_tag="WJL14_001685"
     CDS             61..507
                     /gene="yciA"
                     /locus_tag="WJL14_001685"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ7656731.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
     gene            complement(558..1379)
                     /locus_tag="WJL14_001686"
     CDS             complement(558..1379)
                     /locus_tag="WJL14_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ7656732.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYCVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFGDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1498..1956
                     /locus_tag="WJL14_001687"
     CDS             1498..1956
                     /locus_tag="WJL14_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ7656733.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PEFTRMGKMVKGEEPYDVEKFKQAAASFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2120..3235)
                     /gene="lst"
                     /locus_tag="WJL14_001688"
     CDS             complement(2120..3235)
                     /gene="lst"
                     /locus_tag="WJL14_001688"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ7656734.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFKQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3336..3440
                     /locus_tag="WJL14_001689"
                     /pseudo
     CDS             3336..3440
                     /locus_tag="WJL14_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            3668..5893
                     /locus_tag="WJL14_001690"
     CDS             3668..5893
                     /locus_tag="WJL14_001690"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ7656735.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWAKDSKTHVATMQSGDFFHNEQSVTVPDATSVSIVFTDKQGNKKELRDPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVVDAAGKVLMQHD
                     VEAGDIWRMCQTKDDPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1610 a         1745 c         1338 g         1298 t
ORIGIN      
        1 gtaacgccgt actggtttaa atttaatcca ctatatattt caataaaaca aggaaacatt
       61 atgcagcacg aagaaggcaa ccgccaacgc cctcaaggcg aactgctcct gcgtaccgtc
      121 gctatgccgc gcgataccaa tcccaaccaa gacattttcg gcggctggat tatgtcgcag
      181 atggatttgg gcggcggcat attggcggcg gaaatcgcgc ggggacgcat cgttaccgtc
      241 gccgttcagg aaatgaactt catccgcccg gtcaaggtcg gcaacgtcgt ctgctgctac
      301 gggcattgcg tccgcgtggg caatacttcc ctccagctta aagtcgaagt ctgggtgaaa
      361 actttgatga acgattgcgt taccgaagac cgccacctcg taaccgaagc cgtgttcact
      421 tatgtcgcca tcgatgcgga aggcaatccg cgcccgattc cgaaagaagg caaccccata
      481 ttggcgggct tattgcctac tccgtaaaaa tacccgtaaa aatgccgtct gaaactgtct
      541 tcagacggca ttccgcatca tttgaaccaa gatctgatgc gggaaaacag cgacatactc
      601 tgtttttcaa agtcttcccg cacttccggc acggcttttt ttatcacctt cattcctgca
      661 aaagtctgcg ccacgggcat aatttcaatc gtgttcacat tcatatgcgc cggccggcgg
      721 tacagccaca atgcggtttc cgcaatatct tcggggcgga taaattccac accctcatac
      781 acgcctgctg ccttctcgtc atcgcctttg aagcgcacgt tggaaaactc cgtattgccg
      841 cacaaacccg gctcgatatt ggtaacgcgg atgttcttat ccgccaactc cgcgcgcaaa
      901 ttcaggctga actggcgcac aaacgccttg gttgccccgt aaacgttgct gccggcataa
      961 gcataattac ccgcaatcga acccaaattc atcacatagc cgccgccgcg ttccaccatt
     1021 tgcggcaaaa tcttgcgcgt cagaaacgtc aaacccaaaa cattggtttg aatcattgtt
     1081 tcccaatctc caaaatccgc cttgtccgcc gtgtccaaac ccaaagccag cccggcattg
     1141 ttgatgaggc agtcgatgtc ggaaaattca tcggggatgc cgtttaaggc attttccacc
     1201 gattcgcggc gcgacacgtc catttccaaa gggtaaaaca aagcacccaa ttcatccgcc
     1261 aaggcctgaa gcctgtccgc acggcgcgcc gcaccgataa cgcagtatcc cgccccgaca
     1321 aacgcgcggc acatcgcttc gccgaatcct gccgaagcac cggtaattaa aacagccatt
     1381 gtttttcctt tctttctcgt ttcccaaacc tgtttcaggt atcataacac ccttcagacg
     1441 gcatcgcgcc ggctgaaaat gacaacaacc ataccaccac gacaggacac attcaatatg
     1501 aaaacccaaa tcagccttgc tgctgccgcc atcacactgc tcctttccgc ctgcggaggc
     1561 agcggaatac cttcccaacc caaaggcgaa atttccgaaa accgcaccgc cgcgttcaaa
     1621 tccatgatgc ccgaattcac acgtatgggc aaaatggtca aaggcgaaga accttacgat
     1681 gtcgaaaaat tcaaacaggc ggcagcgtcg tttgccgaaa gcagcaagaa accgttcaca
     1741 ctttttgagt ccgatccgca aggcaacggg cgcgcactgc cagccgtttg gtcggatggt
     1801 gcaaaatttg aagccgaaaa aacaaaattc gccgccgccg tcgaaaaact caacgccgcc
     1861 gcccaaaccg gcaaactgga tgaaatcaaa gccgcctacg gcgaaaccgg cgcaagctgc
     1921 aaatcctgcc acgacagttt ccgcgcgccg gaataacatc cgaaacagaa cgatgccgtc
     1981 tgaagccttc agacggcatt ttctttgtca tttcagcaca cgcaaagcaa tcagaaatta
     2041 tcttcaattc aaaaaatacg ccatatcctg atttttgcct tacttactcc gccattttct
     2101 attttgattt gctatgcgat taatctttat cgtcaaatgt caaaatcggg atgccggatt
     2161 gggtaaacag ggcatacacc ggcttgagcc aataatcttc cggaagggaa gccggtttca
     2221 atgcgtaaac gtgcacattg ggaaaatcct tcatcgtcaa cgccgcgccg ctgaaaaagg
     2281 tataaatttc atacctcgta tgcgggtttt tcttaatctc gcgcaaaata tagtcttcga
     2341 tgacataggg cgaatttaat gtggttacgc cggaaagccc gtaggtttgg cgggggtgcg
     2401 gtgcgacata ttgtatgttg aagttttttg ccgccttttc cgaaatttcc ttcatctcct
     2461 tgtcgggcga acccaaaagt atccgcaccg tgccgcccgt ttcgtccccc gccttcagtt
     2521 cggacgcatc gaacagcggc aggtaagtca tcttgcggcg gccgtcgtcc ataatgtttt
     2581 tcaaaccctt gaatatcgtg taatgctcgt cggaagcatt acgggttttg gcgatgctcc
     2641 aatctccgat catcatccgg gcaaaattcc gcttgatcgt cccgtttaca gaaaactcat
     2701 cgcccaaata gctgctgctt tgaattaaat tgcctgtccc gtcgtcaaag gttttgattt
     2761 ccgcatccgg gtaagtgctc aaaaaggcgg caatgctgac tttttccaaa cttgccaaat
     2821 aaatccgttt gaccttcggc agcagcatcg actttacctt cagctccgcc atcgtcggaa
     2881 tgaaattaaa cgatttgttc aaaccgtagg gcaggtggaa aaagtacgcc cgctccgctt
     2941 tatcctttat ctgcttgaaa taataatcgt atttttcatt cctgttttca gacatcagca
     3001 ccacataaaa ccgctccccc ggatgctgcg ccataatcct ttccgccacc ttcatctgca
     3061 atatggtata gcagaaaatc agattgaccg gttccccctc ttcattgaag agtttgtcct
     3121 tcagcaggga aaccgcattc ctttccccat gatttacccg gtcaaatgta taaaatatcc
     3181 cgaaacaaaa aacaatcaaa cacaacacgg tcaaacaagc ctttttcaag cccatatccc
     3241 taaaactcca ttccgacaaa ttgaacatac gccccgagtg tcccgggacg gaaaaatgcg
     3301 gaattttagc agatattccg acagttaccg aatattatag tgatttaaat ttaaatcagg
     3361 acaaggcgac gaagccgcag acagtacaag cagtacgaca aggcgaggca acgctgtact
     3421 ggtttaaatt taaaccacta taaaatcaat ttcaccgaaa atatgccgcc gcacatcagg
     3481 cacagcacct ccgaccgccg ccaaacgcgg gaaaaccatt tcccccttcc tttcatgcag
     3541 ccgcgttcaa acacgccaag atcgaacaaa gcgcgaacaa agccgccaaa atatgccata
     3601 atagccgcgc acattccccg cgcaatgtgt tccaaaagaa aaaaccaacc tacaagagaa
     3661 taatcctatg actcaaaaat ccaccattgt ttatacccat accgacgaag cccccgcgct
     3721 ggcgacccaa tcgctgctgc cgattgtgca ggcgtttgcc cgccacgccg atattgatgt
     3781 caaaactgcc gacatttccc tgtccggccg tattttggcg gcattccccg aatatctgac
     3841 cgaggcgcag cgcgtacccg acgcgcttgc cgaattgggc gaactggtga aacaacccga
     3901 tgcaaacgta atcaaactgc cgaacatcag cgcgtccgta ccgcaactga ctgctgcgat
     3961 taaagaattg cagtctaaag gctttgccgt tcccgactat cccgccgacc cgcaaaccga
     4021 cgaagaaaaa gccgtacgcg aacgctacga ccgcatcaaa ggcagcgcgg tcaatcccgt
     4081 tttgcgcgaa ggcaactccg accgccgcgc ccctaaggca gtgaaaaact ttgccaaaaa
     4141 aaatccgcac agtatgggcg cgtgggcgaa agattccaaa acccacgttg ccaccatgca
     4201 aagcggcgac tttttccata acgaacaatc cgttaccgta cccgatgcga cttccgtgtc
     4261 catcgtgttc accgacaaac aaggcaacaa aaaagagctg cgcgatcctg ttgccctgaa
     4321 agccggcgaa atcatcgacg cgaccgtaat gagcaaaaaa gccctgctcg ccttccttgc
     4381 cgaacaagtg aaagacgcga aagcaaaagg cgtattgttc tcgctgcaca tgaaagccac
     4441 gatgatgaaa gtgtccgacc cgattatctt cggacacgcc gtcaaagtgt tcttcgcgcc
     4501 tgtttttgaa aaattcggcg acaaattggc tgccgcaggc gtcaacgtca acaacggctt
     4561 cggcaacctg cttgccaatc tggacaagct ggatgcggac acccgcgccg ccgttgaagc
     4621 tgaaatcgct gccgtttacg ccgccaaccc tgatttggca atggtcgatt ccgataaagg
     4681 cattaccaac ctgcacgttc ctagcgatgt catcgtcgat gcttctatgc ctgcgatgat
     4741 ccgcaattcc ggccgtatgt gggacaaaaa cggcaaagcg caagacacca aagccgtgat
     4801 tcccgacagc agctacgctg gtgtttacca agcaaccatc gatttctgcc gcgaacacgg
     4861 cgcattcgat ccgacaacaa tgggtactgt gcccaacgtc ggactgatgg cgcaagcggc
     4921 ggaagaatac ggttcacaca acaaaacctt cgaaatcgaa gccgacggtc aggttcaagt
     4981 ggttgatgca gcaggaaaag tcctgatgca acacgacgtt gaagcaggcg acatctggcg
     5041 tatgtgccaa accaaagacg atccggttaa agactgggta caacttgccg tcaaccgcgc
     5101 ccgtctgagc aacacgcctg ccgtgttctg gctcgacgaa aaccgtccgc acgacaagag
     5161 cctgctcgcc aaggttaaag cctaccttgc cgaactggat accaatggcc tcgacatccg
     5221 cgtcctcgct cctgaagaag ccgccaagtt cagcttgggt cgtctgaaaa acggcgaaga
     5281 caccatctcc gtaaccggta atgtcttgcg cgactacctg accgacttgt tcccaatttt
     5341 ggaactcggc acaagcgcga aaatgctgtc tatcgttcca ttgatgaacg gcggcggtat
     5401 gtttgaaacc ggcgcgggcg gttctgcacc gaaacatgtt caacaattcc tcgaagaaaa
     5461 ccatttgcgc tgggactcgc tgggcgaatt cctcgcactc gccgtatcgt ttgaacattt
     5521 ggcgcaaaaa accggcaatg ccaaagccca agtcctcgcc gacactttgg atgcagccac
     5581 cgaaaaactg ctgttgaacg acaaatcgcc taaacgtaaa gccggcgaac tcgacaaccg
     5641 cggcagccat ttctacctca ccctctactg ggcgcaagaa ttggcggcgc aagacaaaga
     5701 tgccgaactg aaagccgcat ttacgccatt ggcagccgct ttgaccgccg acgaagcgaa
     5761 aatcgttgcc gagctttccg ccgtacaagg caaagcggcc gacatcggcg gctactacgc
     5821 cgccaatcct gaaaaagccg cacaagtgat gcgtccgagc gtaaccttta atcaggcact
     5881 aaacgcctta taagcacaaa ggtaaaaatg ccgtctgaac attcgtcgtt cagacggcat
     5941 tagttatccc tatctgcctg attatgatta aacgccgcac ccattggata t
//
LOCUS       DAWKQB010000124         5988 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.124, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000124 DAWKQB010000000
VERSION     DAWKQB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.124"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..79)
                     /locus_tag="WJL14_001691"
     CDS             complement(<1..79)
                     /locus_tag="WJL14_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7656736.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="WJL14_001692"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="WJL14_001692"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ7656737.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKIVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WJL14_001693"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="WJL14_001693"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ7656738.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTVALHYVYNTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRCESEYDAFGVGHSSTSIGAALGMAAADKQLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNIENLVDVLEDLR
                     GRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGVPVSDGMETVEIGKG
                     IIRREGEKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGSAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WJL14_001694"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="WJL14_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ7656739.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDAGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /locus_tag="WJL14_001695"
     CDS             4804..5868
                     /locus_tag="WJL14_001695"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ7656740.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
BASE COUNT         1352 a         1833 c         1560 g         1243 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatac agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctacctccc aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcgt gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt cgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg catcgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt atgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acattgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgca cgacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caacttcacc ggcacgcccg acatgatcaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaaattgt cgaagcctaa accctcacgc
     1621 cgaaaaaatg ccgtctgcag cgtttcagac ggcattttgc cttgtatcgg cagacgacgg
     1681 cgcggccggg cggcttaatt tgccgcatcc cgatccgaca gccacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaag acgggtttgc agatgccgtg tttcgccaac
     1861 acttccagca ccgcgctgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagttttccg gcgaccgcca atgcaggggc gaccatactg
     2041 ccgaaggcaa tgaatgcggt tttctcacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcacg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg cgtctgcctg atagcaggtc gaaagcagca ggcggcattc attttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agcttaaatc gtacaaaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catcccttcg caagccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg cgatgccgac atcgaaatag cggtcgggga atcgttgttc aaactcaacc
     2521 aagccgctgc cctcgcgcat ggcgggggta atcgcaacca gtcgggaatc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacgggat cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcaga
     2761 agctgcgggc ctttgcgtcc gcgcaggtct tccaatacat cgaccagatt ttcgatgttg
     2821 tgtccgtcca cggggccggt atagcggaag ccgaagtttt caaacaaaga cagtgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttat gttcgacttt ttgggcaaac
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 agtccgtgca tatcgcgcac gacgttgctg gcaaggtatt tgggcaacgc accgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgccc
     3121 gcgcagttca aggcttcaaa cgcctgaccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caactgtttg tccgccgccg ccatgcccaa cgccgcgccg
     3241 atggaggtgg aggaatgccc cacgccgaac gcgtcgtact cggactcgca acgtttcgga
     3301 aaacccgcca aaccgccata ttggcgcatg gtgtgcatct ggtttttacg tccggtaaga
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgttg
     3421 taaacgtagt gcagcgcaac cgtcagctcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gtgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccgggtgca acggccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc tgatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccggtcagc ggaacctgcc gctgcttgcg tcctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatat acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagt tcgaacagcg cgtgatcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cggcagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt tgaccagcca ggcgcaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgctgc
     4441 gacaagccct gcggcacgcc gcctgcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agcgcaggtg cgcgcaaata cgcgggcgcg
     4981 ccgtttttgc gccacctgat tttggcggct gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcctgcgg cgtatccgtt
     5221 gaaggcgaaa tcggcgtatt gggcaacctc gaaactggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgta aagtcaacat cgataccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatatttgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tacctcgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaaacc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgttta
//
LOCUS       DAWKQB010000125         5948 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.125, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000125 DAWKQB010000000
VERSION     DAWKQB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.125"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            284..1231
                     /locus_tag="WJL14_001696"
     CDS             284..1231
                     /locus_tag="WJL14_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ7656741.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAG
                     SMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAVAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="WJL14_001697"
     CDS             complement(1384..2508)
                     /gene="rluD"
                     /locus_tag="WJL14_001697"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ7656742.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTLWIKEGAVIVNDKPSQPKDKMIGGEQIRVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2507..3310
                     /locus_tag="WJL14_001698"
     CDS             2507..3310
                     /locus_tag="WJL14_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ7656743.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3368..5587)
                     /locus_tag="WJL14_001699"
     CDS             complement(3368..5587)
                     /locus_tag="WJL14_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ7656744.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHDGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQAAAFLAEYTLRFLVWLAGVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRPPSVPENEAAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEAELVKKYGSNLYSQVLVLGHHGSNTSSSGLFLHTVAPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1454 a         1885 c         1425 g         1184 t
ORIGIN      
        1 ttctccgagc tttatatgtt tcaacagagg acggcacatc aagcaccgca ctgcgtgttg
       61 cccgaacgag actgccctcc gattagattc tatcgctata aacagacggg tttcaaccga
      121 aaaggaatgg gaatgaagtc cgtttccgac acctctcggg cgatgccgtc tgaaaaccaa
      181 tctccacttt cagacggcat tgtttaggca cggtcatttt gcaaattaag gttaaggcgt
      241 gtatgcttgc cttcccttaa aaacaaacac gcaacgcatt attatgaata tcctcagtaa
      301 aatcagcagc tttatcggaa aaacattttc cctctgggcc gcgctctttg ccgccgccgc
      361 ttttttcgcg cccgacacct tcaaatgggc ggggccttat attccttggc tgttgggcat
      421 tattatgttc ggtatgggtt tgacgctcaa accttccgac ttcgatattt tgttcaaaca
      481 tcccaaagcc gtcataatcg gcgtaatcgc acaattcgcc attatgccgg caaccgcctg
      541 gctgctgtcc aaactgttga acctgcctgc cgaaatcgcg gtcggcgtga ttttggtcgg
      601 ctgctgcccg ggcggtacgg cttccaatgt gatgacttat ctggcgcgtg gcaatgtggc
      661 tttgtcggtt gccgttacgt ctgtttccac cctgatttcc ccattgctga ctcccgccat
      721 cttcctgatg cttgccggcg aaatgctgga aatccaagcg ggcagtatgt tgatgtccat
      781 cgtcaaaatg gttttgctcc ccattgtttt gggtttgatt gtccataagg ttttgggcag
      841 taaaaccgaa aagctgaccg atgcgctgcc gctggtttcc gttgccgcca tcgtgctgat
      901 tatcggcgcg gttgttgggg caagcaaagg caagattatg gaaagcggtc tgctgatttt
      961 tgcggttgtc gtactccaca acggcatcgg ctacctgctc ggcttctttg ccgccaaatg
     1021 gaccggtctg ccttatgatg cacaaaaaac gctggccatc gaagtcggta tgcaaaactc
     1081 gggcctggcc gccgcgcttg ccgccgcaca ctttgccgtt gcgccggtcg ttgccgttcc
     1141 gggcgcattg ttcagcgtgt ggcacaatat ctccggctcg ctgctggcaa cttattgggc
     1201 ggccaaagcc ggtaaacata aaaaacccta aacgcctttc atcataatgc cgtctgaacg
     1261 gggcaaacga aaccggcggt ttccgcccga ccccttcaga cggcctttgt ttgaaatcgg
     1321 cgggaacgat tgcccggctg ctccggttcg gctgcctgcg ttcgccccgc ctttcaagcc
     1381 gcctcactcc cttacataaa ccacttcgac atcgtagtcg tcttcgttcc aatcgtcatc
     1441 gtcgtccgcg ccgaatttgt cctgccattc ctcttcattg ctcaaagacg aatccaaacc
     1501 ggcttcaaga cggaggacag acaataaatg atatatgtcg tccggaatcg gtgcctcaaa
     1561 cgaaacggtt tcgccgcttt ccggatgggt aaaactcaag cggtaggcgt gcaacgcctg
     1621 acgcgcgccc agacttttaa cggcttcttt caccgtgtcg ccgcacgggt ggcgcgggtt
     1681 gccgtaaacc gggtcgccgg caagcggatg gttggcctca cgcatatgga cgcggatttg
     1741 gtgcgtcctg cctgtttcga gcgagcattc gatatagctg tgcgccagat agcgttccaa
     1801 cactttgacg tgggtaacgg ctggtttgcc gccgaatttg acgactgcca ttttcaggcg
     1861 gttgtgcgga tcgcgtccga tttgggtttc gattttaccg tcaaagggaa cgatgccgtt
     1921 ggcgacggcg cggtaaatgc gtttgaccgt gcgttcctga agctgccgca caagggaatt
     1981 ttgcgccggc agggttttgg caaccaccat cagcccgctg gtttccttgt cgaggcggtg
     2041 gacgatgccc gcgcgcggaa tttgggacag ctcggggcaa tgcgccaaca ggccgttgag
     2101 cagcgtcccc gtccagttgc ccgccgccgg atgcaccacc agtccggccg gtttgttgac
     2161 gacgatgacg gtatcgtctt cgtaaacaat atccaaagcc ataggctctg gaacaaacgc
     2221 cagattttcc tcactcggac ggacggttac acggatttgc tcaccgccta tcattttgtc
     2281 tttgggttgc gaaggtttat cgtttacaat aaccgcgccc tctttaatcc ataatgtcag
     2341 gcggctgcgc gaatagtcgg gcagaagttt cgccaacacc gcatccaacc gcccgccggc
     2401 aagctcgagc ggaacggtca gaccgatacg gttttcagtt tcggaagctg acgcaaagtc
     2461 tgaatcgtcg ctataatcgg attcattatc aaaggaagta ttctgcatga aaaaaattct
     2521 tttaacggtt tcattaggtt tggcactgag tgcctgtgcc actcaaggta cggtcgataa
     2581 agatgcccag attacccaag attggagtgt ggagaagctc tatgccgaag cccaggacga
     2641 gctgaacagc agcaattata cgcgggctgt caagttatac gaaatcttgg aatcgcgctt
     2701 ccccaccagc cgccatgccc ggcagtcgca actggatacc gcatacgcct attataaaga
     2761 cgatgaaaaa gacaaagctc tggcggcaat cgaacgcttc cgccgccttc acccgcaaca
     2821 cccgaatatg gattacgcgc tgtatctgcg cggcttggtg ctgttcaacg aagaccagtc
     2881 cttcctgaac aaactggcct cgcaagactg gtccgaccgc gacccgaaag ccaaccgcga
     2941 agcgtaccag gcgtttgcgg aactcgtcca acgcttcccc aacagcaaat acgccgccga
     3001 tgcgaccgca cgcatggtca aactggtcga tgcactgggc ggcaatgaaa tgtcggtggc
     3061 gcgctactac atgaaacgcg gcgcatatat cgccgccgcc aaccgcgccc aaaaaattat
     3121 cggcagctac caaaatacac gctatgtcga agaatcgctc gccatcttgg aacttgccta
     3181 ccaaaaactc ggcaaaccac agcttgccgc cgatacgcgc cgcgtgttgg aaaccaactt
     3241 cccgaaaagc ccgtttttga cgcacgcttg gcagcccgac gatatgcctt ggtggcgtta
     3301 ctggcattaa gcccatcctc gaaatgccgt ctgaagccct ttcagacggc attttatgtt
     3361 tcaaacctca ttcaaacggt tttttctgcc aatagaattt ataccctctc aaacgttgag
     3421 ccttcacgcc gccgcgcccc aagccgaatt gcagcgcgcc cgacaaatcg gtacgcagca
     3481 gtttgatgcc gtgtgcgcgg acacggttct gcaccgcttc ggtcggatgt ttgtaggcgt
     3541 tggcataacc gcttgacgct accgcatatt ccggtgcgac cgtatgcaaa aacaaccctg
     3601 atgatgaagt attgctgccg tgatgcccca ataccaacac ctggctgtac aggtttgagc
     3661 cgtatttttt aaccaactcc gcctcgcctt tcgtatccaa atcgcccgtt accagcagtg
     3721 ccgcaccacc agccacaaca cgcaaaacac aacttttccc attatcatcg atgtttttgc
     3781 gttcagacgg catcaaaaac tcgaaatcca cgccgtccca ttgccaacgc tgttccgcac
     3841 aatgccgcgc cccctcataa aattctggct gcccggcata aatcccgccg ttcggtatct
     3901 tgccgactgc ctgaaaaccg ccgtcgtggt cgctgtcgtg atgcgacaga accagcttgt
     3961 ccaaacggcg gacacccgcc gcattcaaac tcggcacaat ccccatctgc gccgccgctg
     4021 ccgttccggt gtcaaacaaa agatgatgat ttgccgtctg aaccgacacc gacaaaccct
     4081 gccccgcatc ccaaaccgta accgcagcct cattttccgg cacgctgggc ggacggtaaa
     4141 acacaaaccc tgccaacagc aacaccgccc acggacgcaa acccaagccg cgcggcagca
     4201 acaacagcaa agcggcacac accgccaaca ccagcagcgg caaaggtgcg gcggcaacgg
     4261 caaactccgg cgacacaccg gcaagccata ccaaaaacct caaagtatat tctgccaaga
     4321 atgctgccgc ctgctgaagc ggcgcaaacg gcacgaccga acccagcaac gccagcggcg
     4381 tcaataccca agaaaaccac ggaatcgcca ccgcattgac caaagggctg attaaaggca
     4441 gcgaagcaaa cagataaccg agcaaaacca acgacaacac cgaagccgcc cactgcccgc
     4501 gcaaagcagt ttgccgtttc ccctcgtgca aacgccccga acacgcccat atcagggccg
     4561 ccaccaaacc aaaagacagc caagtcccca cacccaagac cgccgacggg tcgaacagca
     4621 gcaccgccgc caacgcctgc caccacgtcg cccacgccga cagtcttccc ctacgccaag
     4681 cccacgcaaa cgccgccaac atcaaaacac tgcgctgcgt cggcacggaa aaaccggcaa
     4741 gcagcgcgta aaacagcgcg cctgcacacc ccgccgccaa aacccacacg cgcggccggg
     4801 caggaaggcg cggggaacag gcaagcagcc gcttcgccag ccacgcaaac atcaccgcca
     4861 ccatcgtaac gtgcaaaccc gaaatactga ccaaatgcgt cagccccaac ggccggaacg
     4921 cctgccacaa ttcggggcgc aatgccgact gttcgcccac gctcaacgcg cgcatcagcc
     4981 cgatgccgtc tgaaagcccg ccgtccgcat ccgcctgctg ccaattacgg ctgatgcggc
     5041 tgcgccaaac cgcaatcccc caaccgcttc cgtcatgcag caaaaccctg tccgcaccga
     5101 ccgtccccgc gccgcctatc ccgttggata atgcccacgc ctcacggttc aaaccgcgca
     5161 ggttcaactc cccgacgacg gggtgcacac gtgccgttat ccgccatctg ctcccgaccg
     5221 cccattcgcg ccgtttgtag tccgacagca gcaaatcaaa cgtccgacca ccgctgtcca
     5281 cagcctttgc cgcaaactgc acgcgccgcc cgtccgacct cggcatatcc gccacttcca
     5341 ctgtcaaagg cacgcctgaa accgcctccg cccgccattg cgaagacaat gccgcctccg
     5401 tcctgaatac gccgtaagcc gcgcccgcca acacgcacag catcagaccg gcaaacgcaa
     5461 aacgccttgc aaacacagca aaaaccgcaa aagccgccag ccaaaacagc caatgcggca
     5521 cgaccggcag cgcaaacgaa gccgccgcgc cgacaaccca acacggcaaa aaccaacgac
     5581 ggaacacgtc tccccacccc agatatttca aaaataaacg gataataaag caaatccgaa
     5641 aaaccgtcat aacaagattt gaaaatgata tagtggttta aatttaaatc aggacaaggc
     5701 gacgaagccg cagacagtac aagcagtacg acaaggcgag gcaacgctgt actggttttt
     5761 gttaaaccac tataagaatg caaacgaaaa gcctcagtat tatccgagac aacggcaaac
     5821 tttccatcgt cattcccacg aacccacatc ccgttattac cacgaaagcg ggaatctagg
     5881 actcagggtt aagaaaacct acatcccgtc attccctcaa aaacagaaaa ccaaaatcag
     5941 aaacctaa
//
LOCUS       DAWKQB010000126         5928 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.126, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000126 DAWKQB010000000
VERSION     DAWKQB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.126"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            55..1593
                     /gene="murJ"
                     /locus_tag="WJL14_001700"
     CDS             55..1593
                     /gene="murJ"
                     /locus_tag="WJL14_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ7656745.1"
                     /translation="MNMLGALVKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGMAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHAGLSLAIGLGACINAGLLFFLLRRHGIYRPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1726..2682
                     /locus_tag="WJL14_001701"
     CDS             1726..2682
                     /locus_tag="WJL14_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ7656746.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRSAPAYRRELLPDSTGKT
                     KTAYDFSDGISPDAPLVVLFHGLEGGSGSHYAVELMLAVRDRGWNGVVVHFRSCGGVA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAV
                     TLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2733..3515
                     /gene="trpC"
                     /locus_tag="WJL14_001702"
     CDS             2733..3515
                     /gene="trpC"
                     /locus_tag="WJL14_001702"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ7656747.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPPEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADDIEAEVGKLF"
     gene            3580..5880
                     /gene="recQ"
                     /locus_tag="WJL14_001703"
     CDS             3580..5880
                     /gene="recQ"
                     /locus_tag="WJL14_001703"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ7656748.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQESIVNALAGGRSLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVANLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLAVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNILRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVYGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1278 a         1755 c         1633 g         1262 t
ORIGIN      
        1 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaat
       61 atgctgggag ctttggtaaa agtcggcagc ctgacgatgg tgtcgcgcgt tttgggattt
      121 gtgcgcgata cggtcattgc gcgcgcattc ggcgcaggca tggcgacgga tgcgttcttt
      181 gtcgcgttca aactgcccaa cctgcttcgc cgcgtgtttg cggagggggc gtttgcccaa
      241 gcgtttgtgc cgattttggc ggaatataag gaaacgcgtt ctaaagaggc gacggaggct
      301 tttatccgcc atgtggcggg gatgctgtcg tttgtactgg tcatcgttac cgcgctgggc
      361 atacttgccg cgccttgggt gatttatgtt tccgcacccg gttttgccaa agatgccgac
      421 aaatttcagc tctctatcga tttgctgcgg attacgtttc cttatatctt attgatttca
      481 ctttcctctt ttgtcggctc ggtactcaat tcctatcata aattcagcat tcctgcgttt
      541 acgcccacgt tcctgaacgt gtcgtttatc gtattcgcgc tgtttttcgt gccgtatttc
      601 gatcctcccg ttaccgcgct ggcttgggcg gtttttgtcg gcggcatttt gcaactcggc
      661 ttccaactgc cctggctggc gaaactgggt tttttgaaac tgcccaaact gagtttcaaa
      721 gatgcggcgg tcaaccgcgt gatgaaacag atggcgcctg cgattttggg cgtgagcgtg
      781 gcgcagattt ctttggtgat caacacgatt ttcgcgtctt atctgcaatc gggcagcgtt
      841 tcatggatgt attacgccga ccgcatgatg gaactgcccg gcggcgtgct gggggcggca
      901 ctcggtacga ttttgctgcc gactttgtcc aaacactcgg caaaccaaga tacggaacag
      961 ttttccgccc tgctcgactg gggtttgcgc ctgtgcatgc tgctgacgct gccggcggcg
     1021 gtcggaatgg cggtgttgtc gttcccgctg gtggcaacct tgtttatgta ccgtgaattc
     1081 acgctgtttg acgcgcagat gacgcaacac gcgctgattg cctattcttt cggtttaatc
     1141 ggtttgatca tgattaaagt gttggcgccc ggcttctatg cgcggcaaaa catcaaaacg
     1201 cccgtcaaaa tcgccatctt cacgctcatc tgcacgcagt tgatgaacct tgcctttatc
     1261 ggcccgttga aacacgccgg gctttcgctc gccatcggtt tgggcgcgtg catcaatgcc
     1321 ggattgttgt tcttcctgtt acgcagacac ggcatttacc ggcccggcaa gggttgggcg
     1381 gcgttcttgg cgaaaatgct gctctcgctc gccgtgatgg gcggcggact gtgggcggcg
     1441 caggcttgcc tgccgtttga atgggtacgc gccggcggaa tgcggaaagc ggggcagctc
     1501 tgcatcctga ttgccgtcgg cggcggactg tatttcgcat ctctggcggc tttgggcttc
     1561 cgtccgcgcc atttcaaacg cgtggaaagc tgaccgatgc cgatatcctt tttcggaaaa
     1621 cgctttttcc gcgcctatat tcgggcgcgc atcccccgcg tccgcaccgg ccggacgggc
     1681 tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat accttatgat tttgacaccg
     1741 ccggacacac cctttttcct ccgcaacggc aatgccgaca cgattgccgc caaattcctg
     1801 caacgctccg cacctgcata ccgccgcgag ctgcttcccg acagcacggg taaaaccaaa
     1861 accgcctacg acttttcaga cggcatttcg cccgatgcgc cgctggtcgt gctgtttcac
     1921 ggtttggagg gcggcagtgg cagccattac gcggtcgaac tgatgctcgc ggtacgcgat
     1981 cggggttgga acggcgtagt cgtccatttc cgcagctgcg gcggcgtagc gaacaccgcc
     2041 ccggtgttct accacttggg cgataccgcc gaaattgcct ttactttgga cacgctcgcc
     2101 gcgcgttacc gtgaaatata cgccgtcggc gtatcgctgg gcggcaacgc gctggcaaaa
     2161 tatttgggcg aacagggcga aaacgcgctg ccgcaagccg ccgccgtcat ctccgcaccc
     2221 gtcgatgcag aggcggcagg caaccgcttc gacagcggca tcacacggct gctctacacg
     2281 cgctacttcc tccgcacact gatacccaaa gcacggtcgc tccaaggttt tcagacggca
     2341 tttgccgcag ggtgcaaaac actgggcgag tttgacgacc gtttcaccgc accgctgcac
     2401 ggctttgccg atcggcacga ctactaccgc caaacttcct gcaaaccgct gctcaaacac
     2461 gttgccaaac cgctgctcct gctcaatgcc gtcaacgacc ccttcctgcc gcccgaagcg
     2521 ctgccccgcg cagacgaagt gtccgaagcc gttaccctgt tccagccgac acacggtggt
     2581 catgtcggct ttgtcggcag caccggcggc aggctgcacc tgcaatggtt gccgcagacc
     2641 gtcctgtcct atttcgacag cttccgcaca aacaggcgtt aacggtttga tgctaatatt
     2701 ccccctttcc ccagacgaat acggaacacg acatgaccga catcctcaat aaaatcctta
     2761 ccgccaaggc acaggaagtt gccgcccaaa aagccgccgt caacgccgaa cacatccgcg
     2821 cacttgccgc agaagccgcg cccgtccgca gctttatcgg ttcgatacgc ggcaaacacc
     2881 gcctcaacct gcccgccgtc attgccgaaa tcaaaaaggc aagcccgagc aaagggttaa
     2941 tccgtccgga cttccgaccg cccgagattg cacgcgccta tgaaaacgcc ggggcggcgt
     3001 gtttgtccgt actgaccgac gaaccctatt tccaaggttc gcccgaatac ctcaaacagg
     3061 cgcgcgaagc cgtatcgctg cccgtgctgc gcaaagactt catcatcgac gaatatcagg
     3121 tttatcaggc gcgcgcctgg ggggcggatg ccgtcctgct gattgccgcc gcactggaac
     3181 gggaacaatt ggaacgcttt gaagcggtgg cgcacgaatt gggcatgacc gtcctgctcg
     3241 agctgcacga tgaaagcgaa ttggaaaaat gccgcaacct gaccacgccg ttgtggggcg
     3301 taaacaaccg caacctgagg acttttgaag tctccctcga ccaaaccctg tcgctgctgc
     3361 ccgcgctgga aggcaaaacc gtcgttaccg aaagcggcat tacaggtaag gcggatgtgg
     3421 aatttatgca atcgcgcggc gtgcatacct tcctgatcgg cgaaacgttt atgcgtgccg
     3481 acgatattga agcagaagtg ggcaaactct tctaaatccc gatttcagac ggcatattgc
     3541 cgccgaccga tcaacatcct taccgagcac ggaaccatca tgcaccgacc gaccgccaaa
     3601 cagattttgc acgaagtatt cggttatccc gaatttcgcg gcaggcagga gtctatcgtc
     3661 aatgctttgg caggcggcag gagtttgatg gtgctgatgc cgacgggcgg gggcaagtct
     3721 ttgtgttacc agattcccgc gctgatgcgc gaaggcgtgg cggttgtcgt atcgccgctg
     3781 attgcgctga tgaacgacca agtggcaaac ctgcatgtgg ccgggattga agcggcggca
     3841 gtcaacagcg gcacatcggc agacgaggcg cgcgagattg ccgacaagct tgcccaaggc
     3901 cgtctgaaac tgctttatgt tgcgccggaa cgcttggtta ccgaccgctt tttgcgtttt
     3961 ctcgaccaac aaaccgtcag tctgtttgcc attgatgagg cgcattgcgt cagccaatgg
     4021 ggacacgatt tccgccctga atatcaacag ctcggcatgc ttgccgaacg ctatccgaac
     4081 gtcccgcgca tcgctctgac cgctaccgcc gatgccgcca cgcgcgccga catcaagcat
     4141 tatctgcact tggacgatgc gcccgaattt gtctccagct ttgaccgtcc gaatatttat
     4201 tatcaggtta tcgaaaaaaa caacggcaaa aaacaattgc tggatttcat ccgcaaagaa
     4261 atgacggggc aaagcggcat tgtgtattgc ctaagccgca aaaaggtgga agatgtggcg
     4321 cagtttttgc gtgaaaacgg attaaacgcg attccgtatc atgctggttt gagcatggac
     4381 gtacgcgagg aaaaccaacg ccgctttacg catgaagaca atattatcgt ggtggcgacc
     4441 gtggcgttcg gcatgggcat agacaaaccc gacgtgcgct ttgtcgccca tctcgatatg
     4501 ccccagagtg tcgaacattt ctatcaagaa tcggggcgcg ccggacggga cgggctgccg
     4561 gccgcaagct ggctgtgtta cggtttgaac gattgggtgt tgctgcgcga acggattgcc
     4621 gaaggcaaca gcgacgaggt gcaaaagcaa atcgaaatgc aaaaactcga tgccatgctt
     4681 gccgtctgcg aaaccgccgc ctgccgccgc gtactgctgc tcaaacattt cggcgaagca
     4741 tccgaaccct gcggccattg cgacaactgc ctgcatccgc ccgtacggtt tgacggcacg
     4801 gtgttggtgc aaaaattact cagctgcgtg taccgcgccg gacaacgttt tgccgccggt
     4861 tacatcacca acattttacg aggtaaaagc gacgattgga tacgcggcaa ccggcacgaa
     4921 caactgtcca cattcggcat cggtacggag ttgtccgaca aagaatggcg cagcgtcatc
     4981 cgccagtgta tcagcctcgg ctacctcacc gtcaacatta cccgatatca ggcattgcaa
     5041 ctgaccgaag ccgccaaaaa agtcctcaaa ggcgaaaccg aagtgatgct gcgtccgctc
     5101 aagcgcgaca agcccgccgc ccgcaccctc aaagacaact ggctgcgtac cgaacgcgaa
     5161 gaacgcctgt ggcaggcatt gcgcgtttgg cgtatgaaac aggcagaagc cgaaggcatc
     5221 cccgcctata tgattttcgg cgacaaaacc ctgcgcgacc ttgtcgaaaa aatgccgcaa
     5281 gacctcaacg gtctgcacga catctacggt ttgggcgaag ccaaaatcga ccgtttcgga
     5341 cacggcatcc tcgaagtctg ccgcaacgcc gccggcttca gccgcgacgc ggtcatccgt
     5401 ccgcaaaccg aacgcgaaca acaactgcgt caaaaactcg aagcctggcg gtatgaacag
     5461 gcaagggcgg aaaactgcgc cctgcatgcc gtcctctccg acgaaagcct tgccgatatg
     5521 cttgccgata cgcccgaaac cgaaaccgac ctcgaaggcg tgtacggctt gggcagcgta
     5581 cgcgccgcca aatacggacg ggacatcctc gccgtctgcc gtccgttttc agacggcatc
     5641 gatgaaaccg ccaaacgcaa acgccgcctg atgcgcgccc tgatccaatg gtgcggcgaa
     5701 acggcaaaac acgaacagtc cgaaccctac cgcattctca gcaaagccgc actgcgcgcc
     5761 attgccgcca aacagccgga aggcttggcg gagcttgccg ccgtatacgg cgtaggcgaa
     5821 gaaaaagccg cacgttacgg tgcggcggtg ttggcggtat tggaacgaaa tgccgtctga
     5881 agcccgttac caagtttcag acggcattgc ctctatttaa aaattcct
//
LOCUS       DAWKQB010000127         5895 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.127, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000127 DAWKQB010000000
VERSION     DAWKQB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.127"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..403
                     /locus_tag="WJL14_001704"
     CDS             20..403
                     /locus_tag="WJL14_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ7656749.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKLA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            518..991
                     /locus_tag="WJL14_001705"
     CDS             518..991
                     /locus_tag="WJL14_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ7656750.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            1117..2220
                     /gene="mnmA"
                     /locus_tag="WJL14_001706"
     CDS             1117..2220
                     /gene="mnmA"
                     /locus_tag="WJL14_001706"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ7656751.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIVGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDDETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            2291..3961
                     /locus_tag="WJL14_001707"
     CDS             2291..3961
                     /locus_tag="WJL14_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ7656752.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSELRLILGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLVVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVKKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            4073..5707
                     /locus_tag="WJL14_001708"
     CDS             4073..5707
                     /locus_tag="WJL14_001708"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ7656753.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1543 a         1584 c         1492 g         1276 t
ORIGIN      
        1 acattttgaa aggataccga tggaaccttc ttcttacgcg gcagaaaaaa aaggcaaagg
       61 cggcatcagg cgcgtcatta acgcattcgg ctattcgata gacggcatcg ccgccgccta
      121 ccgttacgaa gcggcattcc gtcaggtttt gtggctgaac gtactgctgg tgtgcgcggc
      181 atttttttgg gtttccgaaa ccgccgtccg cctgccgttg attatcgcgt cttttgtgtc
      241 ggtcattgtc gaactgttca acaccgccgt cgaagctgcc gtcgatcata cttcgaccga
      301 aaaacacgag ctggccaaac tcgccaaaga cgcaggttct gccgcacaat tgttcgcgat
      361 gctgatgttg gcggcggttt ggctgtctgc cctgttcggg taaaacgctt gcagcaggat
      421 ttgtaatcct ttaggattag tatgtggtat ttattattca cttaatccgc tataagatta
      481 agcacattcc ccgtctgata ttataaggag cagaaagatg aaaaaattat tggcggccgt
      541 gatgatggca ggtttggcag gcgcggtttc cgccgccgga gtccacgttg aggacggctg
      601 ggcgcgcacc accgtcgaag gtatgaaaat aggcggcgcg ttcatgaaaa tccacaacga
      661 cgaagccaaa caagactttt tgctcggcgg aagcagcccc gttgccgacc gcgtcgaagt
      721 gcatacccac atcaacgaca acggcgtgat gcggatgcgc gaagtcaaag gcggcgtgcc
      781 tttggaggcg aaatccgtta ccgaactcaa gcccggcagc tatcacgtga tgtttatggg
      841 tttgaaaaaa caattaaaag agggcgacaa gattcccgtt accctgaaat ttaaaaacgc
      901 caaagcgcaa accgtccaac tggaagtcaa aatcgcgccg atgtcggcaa tggaccacgg
      961 tcatcaccac ggcgaagcgc atcagcacta atctgctgaa aatatttgaa atgccgtctg
     1021 aaaaagcccg ggctttcaga cggcattttt atgaccgcct ttaaaatgtg ttaaaatctg
     1081 cctttaaaaa ccgccgtttc caaccatatt ctacgcatga atacgacagc aaacccttcc
     1141 aatatcatcg tcgggctctc cggcggtgtc gattcttccg taaccgccgc cctgctcaag
     1201 cagcagggtt atcaagtgcg cggtgtgttc atgcagaact gggaagacga cgacaacgac
     1261 gaatattgca gcatcaaaca ggattcgttt gatgccatcg ccgttgccga tattgtcggc
     1321 atcgatatcg acatcgttaa tttcgccgcg caatataaag acaaagtctt tgcttatttt
     1381 cttcaggaat acagtgcggg gcgcacgccg aatcccgatg tgttgtgcaa cgccgaaatc
     1441 aaattcaaat gctttttgga ttacgccgta gggcagggcg cggataccat cgccaccgga
     1501 cactatgcgc gcaaagaagt ccgcaacggc gtgcattacc tgctcaaagg tttggatcga
     1561 aacaaagacc aaagctattt cctctaccgc ctcaagcctt tccaactcga gcgcgcgatt
     1621 tttccgttgg gcggtttgga aaaacccgaa gtccgccgcc ttgccgccga gttcaaactg
     1681 ccgactgccg ccaaaaaaga cagcacgggc atctgcttca tcggcgaacg cccgtttcgc
     1741 gagtttctgc aaaaatacct gccgaccgac aacggtaaaa tggttacgcc cgaagggaaa
     1801 accgtcgggg aacacgtcgg actgatgttc tacacgctgg ggcagcgcaa aggtttgggc
     1861 atcggcggcg cgggcgaacc gtggtttgtt gcggctaaag atttgacgaa aaacgaactg
     1921 attgtcgtgc aaggacacga ccatccgctg ctctataccc gcagccttgt gatgaacgat
     1981 ttgagtttca cgctgcccga acgcccgaag gcaggacgct atacctgcaa aacgcgttac
     2041 cgtatggcgg acgcgccttg tgagttgcgc tatttggatg atgaaaccgc cgagctggtg
     2101 tttgacgaac cgcaatgggc ggttacgccg ggccagtctg ccgtgctgta cgacggcgac
     2161 atctgtttgg gcggcggtat catccaaacg accgacaaac ccgtcatcat cacacgataa
     2221 aggtaatgcc gtctgaaacg gttttcagac ggcattgttc cgctcaattc cactttaaag
     2281 accgatacct atggaaaaaa tctggttaga cagctacgag aagggcgtca gtgccgaaat
     2341 cgacatcacg caatacaatt ccgtcagcga cgtattccgc caaagcgtgg aaaaatttgc
     2401 cgaccaacct gcttttcaaa acatgggtaa gacgctgact tatgccgaaa ccggcaaact
     2461 ggcaacagat tttgcttctt atctgcaaaa cgtccttaaa ttgcctcgag gagagcgcgt
     2521 ggcaataatg ttgcccaatg tactgcaata tccggttgcc cttttcggca ttttgcaggc
     2581 aggtttggta gcggtgaaca ccaatccgct ctataccccg cgcgagctgg agcatcagtt
     2641 gaaagacagc ggcgcgaccg ccatcatcgt tttggaaaat ttcgccaaca cgctggagct
     2701 ggtgctgccg cgcacgcaga tcaaacacgt catcgtcgcc tctgtcggcg aaatgttcgg
     2761 gctgcttaaa ggttcgctga tcaatttcgt catccggaaa atcaagaaaa tggtgcccga
     2821 ataccgtatt cgggaaaccg tttcctttca gacggcattg aaagaagggg cgaagcacgt
     2881 tttccaacct gtcgcattaa accgcgaaga taccgcgctg ttgcaataca cgggcggcac
     2941 gacaggtgtt gccaaagggg cggtgctgag tcacggcaac atctgcgcca atatgcttca
     3001 ggcaaaagaa tggattaaaa accagttgcg cgagggcaaa gaaaccgtta tcgccgccct
     3061 gccgctgtat catatttttg ccttaaccgt gaatctgatg atttttgcca atgccggctc
     3121 gaaaatcatc ctgattacca acccgcgcga tatgaaaggc tttatcggag aactgaaaaa
     3181 acagcgggtt aatgtattta tcggcgtgaa cacgctgttt aacgcgatgg ttaaccgtcc
     3241 tgattttgcc gaagtcgatt tttcagaatt gcggctgatt ttgggcggcg gtatggcgac
     3301 ccaaaaagcc gttgccgaaa aatggaaaaa aatcaccggc acgcccatcg tcgaagccta
     3361 cggtctgacc gaagccagcc ccggcgtgtg ctgcaacccc ttaaacatcg aatcatacag
     3421 cggcagcatc ggtttgcccg tctcgtccac cgaagtcgaa ctgcgcgacg caaacggcaa
     3481 agaagtcccc gtcggacagc cgggcgaact ttgggtgcgc ggcccgcaag tcatgaaagg
     3541 ctattggaac cgtcccgaag aaaccgctaa agccatagac gcgcgcggtt tcttggaaac
     3601 cggcgatatt gccgtcatgg acgaaaaagg ctggttgaag ctggtcgatc gtaagaaaga
     3661 cctcgtcgtc gtttccggat tcaatgttta tccgaacgaa atcgaggaag tcatcgcgca
     3721 tcacggcaaa gtgatggaag tcgcctgtat cggcgttccc gacgaaaaaa ccggagaagc
     3781 cctaaaagta tttgtcgtca aaaaagaccc gtcattgacc aaagaagaac ttatcgcctt
     3841 ttgccgcacc gaattaaccg catataaagt accgaaaaat atcgaattcc gcgacgagtt
     3901 gcccaagtcc aacgtcggca aaatcctgcg ccgcgagttg cggcaaagta ccgggaaata
     3961 aagaaaagat gccgtctgaa aacagccgtc caccgttcag acggcatccg tccgtttgcc
     4021 agaaccgcgc gcttcacgtt aaaatcacgc attccaacat gggtattcca tcatgaccaa
     4081 attcattttc gtaaccggcg gcgttgtctc ctcactgggt aaaggtatcg ccgccgcttc
     4141 tattgccgcc atcctcgaat cgcgcggctt gaacgttacc atgctcaagc tcgatcctta
     4201 tatcaacgtc gatcccggca cgatgagccc gttccaacac ggcgaagtgt tcgtaaccga
     4261 cgacggcgcg gaaaccgacc tcgacttggg acactacgaa cgctttatcg attccacgat
     4321 gacccgccgc aacagcttca gcacgggaca ggtgtacgaa aacgttatcg ccaaagaacg
     4381 acggggcgac tatttgggcg gcacggttca agtcattccg cacattaccg acgaaatcaa
     4441 acgacgcatc catgaaggcg cggcaggtta cgatgtggcg attgtcgaaa tcggcggcac
     4501 ggtcggcgac attgaatcgc tgccgttttt ggaagccatc cgccagatgc gcagccagtt
     4561 gggacgcaac aacaccctgt tcgcccactt gagctacgtt ccctacatcg ccgctgcagg
     4621 cgaaatcaaa accaagccga cccagcacac cgttaaagaa atgttgagca tcggtttgca
     4681 acccgacatc ctgatttgcc gtatggacag gacaatgcct gcggacgaac gccgcaaaat
     4741 cgccttgttc tgcaacgtgg aagaacgcgc gattgtcggc agctacgatg tggacagcat
     4801 ctacgaatgc cccgaaatgc tgcacgacca aggcatcgac aacatcatta ccgagcagtt
     4861 gcagcttaat gtgcaacagg cggatttgac cgcgtggaaa aaaatcgtcc acgccatcca
     4921 aaacccgaaa cacaccgtca aaatcgccat ggtcggcaaa tacgttgatt tgaccgaatc
     4981 ctacaaatca ctgattgaag ccttgaaaca cgcaggtgtt cacaccgaaa ccgacgtaca
     5041 aattaccttt gttgacagcg aaagcatcga gaaaaacaac ggcgacgttt ctatgctcaa
     5101 agatatggac gccatcctcg ttcccggcgg ttttggttcg cgcggcgtgg aaggcaaaat
     5161 cgccgctgtg cgctatgccc gcgaaaacaa cgtgccatac ttgggtatct gcctcggtat
     5221 gcagattgcg ctgattgaat acgcccgcga cgtggcaggt ttgaaaggtg cgaattccac
     5281 tgagttcgac ctcaaatgcg ccgcccccgt cgttgccctg attgacgaat ggcaaactgc
     5341 cgacggcagc gtcgaaaccc gcgacgaatc cgccgatttg ggcggcacca tgcgtttggg
     5401 tgcgcaagaa gtcgaattga aagcaggcag cctcgccgcc aaaatctacg gcagcgaaca
     5461 catccgcgag cgccaccgcc accgctacga agtcaacaac aactacgttc ctacgctgga
     5521 acaagcaggt ttggtcatcg gcggtgtatc tgccggacgc gaacgcttgg tcgaaaccat
     5581 cgaactgccg aaccatcctt ggttcttcgc ctgccagttt catcccgaat tcacttccaa
     5641 cccgcgcaaa gggcatcctt tgttcaccgc gtttgtcaaa gctgcgttga acaataaaaa
     5701 agcctgataa agcgttactt gatgatcaaa atgccgtctg aaagcctgac aaagtctttc
     5761 agacggcatt ttcgcaaatc ggggattata aaagccatca atttaatttt tatggtttta
     5821 tctaatattt accaactgaa tattgttaag cggggcgtga aactcaagtt gagacctttg
     5881 caaaattccc aaaat
//
LOCUS       DAWKQB010000128         5816 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.128, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000128 DAWKQB010000000
VERSION     DAWKQB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.128"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            334..5769
                     /gene="iga"
                     /locus_tag="WJL14_001709"
     CDS             334..5769
                     /gene="iga"
                     /locus_tag="WJL14_001709"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050177927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ7656754.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGANN
                     GITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQQADADKKVQAFSQVGIVSGRGTLVLNSPDQINPNNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTDRASTITLTGKSLITAPQNLSVYEIRNDYDDDDYYGYYSYRKP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSAQKDAHFSKNNEVVFEDDWINRTFKATEITVNQSASFSSGRNVS
                     NITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVRDRSHLSVSLANHHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     ERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEAE
                     REAQALAVRRKAEAEEAKRQAAELARRHEKEREAAELSAKQRVGEEERRQTAQSQPQR
                     RKRRAAPQDYMAASQDRPKRRGHRSVQQNNVEIAQAQAELARRQQEERKAAELLAKQR
                     AEAEREAQALAARRKAEAEEAKRQAAELAHRQEAERKAAELSANQKAAAEAQALAARQ
                     QKALARQQEEARKAAELAVKQKAETERKTAELAKQRAAAEAAKRQQEARQTAELARRQ
                     EAERQAAELSAKQKAETDREAAESAKRKAEEEEHRQAAQSQPQRRKRRAAPQDYMAAS
                     QNRPKRRGRRSTLPAPPSPSFDSSAYAAPRALHNPDWYENDYEEIPLDALEDENVSES
                     VDTSDKQPQDNTELHEKYENDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVET
                     VSLQPRAAQPRAQAATQPQAQAAAQADAVSTNTNSALSDAMASTQSILLDTGASLTRH
                     IAQKSRADAEKNSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVL
                     TYSDSQHTFDQASGKNTFVQANLYGKYYLNDAWYMAGDIGAGSLRSRLQTQQKANFNR
                     TSIQTGLTLGNTLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLD
                     FAYRFKVGNLTVKPLLSAAYFANYGKGGVNVGGNSFVYKADNQQQYSAGAALLYRNVT
                     LNVNGSITKGKQLEKQKSGQIKIQIRF"
BASE COUNT         1946 a         1404 c         1305 g         1161 t
ORIGIN      
        1 cgtcattccc acgaaccccc atctcgtcat taccacgaaa gcgggaatct aggacgcagg
       61 gttaagaaaa cctacatccc gtcattcccg cacaagcggg aatccaaaaa caaaaagtcg
      121 cagaaattta acggaagaac cgaaccccga aaatcggctt cccgccttcg cttgaataac
      181 agaacaaagg aagcggaaat aaaaacatcg ggattctttt tttaaattga caataaaaca
      241 ccaaaattaa tgatttacac aacaaaaaat agtcaacacc gccaaaccgg cgtaaaatac
      301 aagacattat cacccttaaa acggtaaaac cttatgaaaa ccaaacgttt taaaattaac
      361 gccatatcct tatccatctt tcttgcctat gcccttacgc catactcaga agcggcattg
      421 gtcagagacg atgtcgatta tcaaatattc cgtgactttg cagaaaacaa aggcaaattt
      481 tttgtcggcg caaccgattt atcagtgaaa aacaaacaag gtcaaaacat cggcaacgca
      541 ttatccaacg taccgatgat tgattttagc gttgcagatg tcaacagacg tacattaacc
      601 gtcatcgatc cccagtatgc cgtcagcgtc aaacatgtaa aaggagacga aatctcttat
      661 tacgggcatc acaatgggca cttagatgtt agcaatgatg aaaacgaata tcgttcagtt
      721 gcacaaaatg actacgaacc aaataaaaat tggcatcacg gtaatcaagg tcgtcttgaa
      781 gactacaaca tggcacgcct taataaattc gtaacagaag tcgcacctat tgcaccaacc
      841 agtgcaggcg gcggcgttga aacctataaa gataaaaacc gtttttctga gtttgtgcga
      901 gttggagcag gtacgcaatt tgaatataac agccgctaca atatgacaga gctttcacga
      961 gcttatcgtt atgccattgc aggtacgcct tatcaagacg tgaatgtcac atcgaatcta
     1021 aatcaagaag gcttgattgg cttcggtgat aattcaaaac atcattcccc agaaaaactc
     1081 aaagaagttt tatctcaaaa cgcattgact aactatgctg tgttaggcga tagcggttca
     1141 ccattatttg cttatgataa acaagaaaaa cgctgggtct ttttaggtgc ttacgattat
     1201 tgggcaggct atcaaaaaaa ctcttggcaa gaatggaata tctataaaaa agaatttgca
     1261 gatgaaatca aacaacgcga taacgccggc accatcaaag gttatggcga acatcattgg
     1321 aaaaccacgg gcacaaacag ccatatcggt tcgacagcgg taaggcttgc cggcaatgaa
     1381 aggggtgcaa acaacggaca gaatgttacc tttgaaaaca acggcacttt ggtattggat
     1441 caaaacatca accaaggcgc gggcggtctg tttttcaaag gcgattacac agtcaaaggt
     1501 gcaaataatg gcatcacttg gctaggtgca gggattgatg ttgccgacgg caaaaaagtc
     1561 gtttggcaag tcaaaaatcc gaatggcgac agattggcaa aaatcggcaa aggcacatta
     1621 gaaataaacg gcacaggcgt taaccaaggg caattaaaag tcggcgacgg tacggttatt
     1681 ctgaatcaac aagccgatgc cgacaaaaaa gtccaggctt tctcccaagt cggcattgtg
     1741 agcgggcgcg gtacattggt gctgaacagt ccggaccaga ttaacccgaa taatctgtat
     1801 ttcggtttcc gtggcggtcg tttggatgcc aatggcaatg acttgacttt tgaacacatc
     1861 cgcaacgtgg atgaaggcgc gcgcattgtc aaccacaaca cagaccgcgc ctccacaatc
     1921 acattaaccg gcaaatcatt aattaccgct cctcaaaatc tttctgttta tgaaatccga
     1981 aatgattacg atgatgacga ttattatggc tattacagct atagaaaacc cattccgcaa
     2041 ggcaaagatc tttattacaa aaactatcgt tattacgccc taaaatccgg cggcagcgtg
     2101 aacgcaccga tgcccgaaaa cggacaaacg gaaaataacg actggatttt aatgggcagc
     2161 acacaagaag aagccaagaa aaacgcgatg aaccacaaaa acaatcagcg tattagcggt
     2221 tttagcggtt tctttggcga agaaaacgga aaaggacata acggcgcatt aaaccttaat
     2281 ttcaacggca aaagcgcgca aaaccgtttc ttgttaacag gcggcacgaa tttaaacgga
     2341 aaaataagcg taactcaagg caatgtcttg ttatcaggtc gtccaacacc acacgcaaga
     2401 gattttgtga acaaatcttc agcccaaaaa gacgcacatt tctccaaaaa caatgaagtc
     2461 gtatttgaag acgactggat aaaccgcaca ttcaaagcca cagaaattac ggttaatcaa
     2521 tccgcatcat tctcttccgg cagaaatgta tccaacatta ccgccaacat caccgcaaca
     2581 gacaacgcca aagtaaattt gggttacaaa aacggcgatg aggtttgcgt gcgctcggac
     2641 tataccggtt atgttacctg caacacaggc aacttatccg ataaggcttt aaacagcttt
     2701 ggagcgacac agattaacgg caatgtgaat ctgaaccaga atgcggcatt ggtcttgggc
     2761 aaggctgcat tatgggggca aattcaaggg caaggaaaca gccgtgtcag cctaaaccaa
     2821 catagcaaat ggcatttgac tggcgacagc caagtacaca atctgtcatt ggcggatagc
     2881 catattcatt tgaacaatgc ctccgatgcg caaagtgcaa atcaatacca cacgctcaaa
     2941 atcaatcatt tatccggtaa cgggcatttt cattatctga cgcacttggc ggaaaatctt
     3001 ggggataaag tgcttgtgaa ggaatccgca tccggtcatt atcagctcca tgttcaagat
     3061 aaaacaggcg aacccaatca ggaagggctg aatctctttg atgcatcatc cgtacgagac
     3121 cgttctcacc tttccgtttc cttggcgaat catcacgtcg atttaggcgc attgcgttat
     3181 acaatcaaaa cagaaaacgg tattacccga ttgtacaatc cttatgccga gaaccgccgc
     3241 cgagtcaaac cggccccgtc tcctgccaca aacacggctt ctcaagcaca aacggacagt
     3301 gcacaaattg ccaagcctca aaatatcgtc gtcgcaccgc ctagcccgca ggcaaatcaa
     3361 gccgaagaag caaaacgcca acaagcaaaa gcggagcaag tgaagcgtca gcaagcagaa
     3421 gcagagcgta aatcggcaga gttagcaaaa caaaaagcag aagccgaacg cgaagcaaga
     3481 gagctggcga cccgccaaaa agcagaacaa gaacgcagca gcgccgaact tgcacgtcga
     3541 catgaaaaag aacgcgaagc ggcagagttg tcggcaaaac aaaaagtcga agccgaacgt
     3601 gaggctcaag cattggcggt acgccgaaaa gcagaagccg aagaggcaaa acgccaagct
     3661 gcagagcttg cacgccggca tgaaaaagaa cgcgaagcgg cagagttgtc ggcaaaacaa
     3721 agggtcgggg aagaggaacg ccgtcaaaca gcccaatccc agccgcaacg ccgcaaacgc
     3781 cgtgccgcac cgcaggatta tatggcagct tcccaagacc gtcctaaacg ccgcggacac
     3841 cgttcggtac agcaaaacaa tgtggaaatt gcccaagccc aagcagaact tgcgcgccga
     3901 cagcaggaag aacgcaaagc ggcagagttg ttggcaaaac aaagggcaga agccgaacgt
     3961 gaggctcaag cattggctgc gcgccgaaaa gcagaagccg aagaggcaaa acgccaagct
     4021 gccgaacttg cacatcgcca ggaagcagaa cgcaaagcgg cagaattgtc ggcaaaccaa
     4081 aaagccgcag ctgaagccca agcattggcg gcacgccaac aaaaagccct tgcgcgtcaa
     4141 caggaagaag cacgaaaagc tgcagagtta gccgtcaaac aaaaagcgga aaccgaacgc
     4201 aaaaccgccg aacttgccaa gcaaagggca gcagccgaag cagcaaaacg ccagcaagaa
     4261 gctcgccaga ccgccgaact tgcacgtcgt caagaagcgg aacgccaagc agcagagttg
     4321 tcggcaaaac aaaaagccga aacagatcga gaagcggcag aatcggcgaa acgaaaagct
     4381 gaggaagaag agcaccgtca agcagcccaa tcccagccgc aacgccgaaa acgccgtgcc
     4441 gcacctcagg attatatggc agcttcccaa aatcgtccga aacgtcgcgg acgcagatct
     4501 actctgccgg caccgccctc gccatcattt gattcgtcag cttacgcagc acccagggcc
     4561 ttgcataatc cggactggta tgaaaatgac tatgaagaaa tccccttgga tgcgttggaa
     4621 gatgaaaatg tatctgaatc agttgacaca tcagacaaac agcctcaaga caatacggaa
     4681 cttcatgaaa aatatgaaaa tgattatgaa gaaatcccct tggatgcgtt ggaagacgaa
     4741 gatgtatccg aatcggttga cacatcagac aaacagcctc aagacaatac ggaacttcat
     4801 gaaaaagttg agacagtgag tttgcaacca agagccgcgc agccgcgagc ccaagccgcc
     4861 actcagccgc aagcccaagc cgccgcgcaa gctgatgcag tcagcaccaa taccaactcg
     4921 gctttatctg acgcaatggc aagcacgcaa tctatcttgt tggatacagg tgcttcatta
     4981 acacggcaca ttgcacaaaa atcacgcgct gatgccgaaa aaaacagcgt ttggatgtcg
     5041 aacatcggtt atggccgtga ttatgcttcc gcacaatatc gccggtttag ttcgaaacgc
     5101 acgcaaacac aaatcggcat tgaccgcagc ttgtccgaaa atatgcagat aggcggcgta
     5161 ttgacttact ctgacagtca gcatactttt gatcaggcga gcggcaaaaa tacttttgtg
     5221 caagccaacc tttatggtaa gtattattta aatgatgctt ggtatatggc cggcgatatt
     5281 ggtgcaggca gcttgagaag ccggttacaa acgcagcaaa aagcaaactt taaccgaaca
     5341 agcatccaaa ccggccttac tttgggcaat acactgaaaa tcaatcaatt cgagattgtc
     5401 cccagtgcgg ggatccgtta cagccgcctg tcatctgcag attacaagtt gggtaacgac
     5461 agtgttaaag taagttctat gtcagtgaaa acactgacgg ccggactgga ttttgcttat
     5521 cggtttaaag tcggcaacct taccgtaaaa cccttgttat ctgcagctta ctttgccaat
     5581 tatggcaaag gcggcgtgaa tgtgggcggt aattccttcg tctataaagc agataatcaa
     5641 cagcaatatt cagcaggtgc cgcgttactg taccgtaatg ttacattaaa cgtaaatggc
     5701 agtattacaa aaggaaaaca attggaaaaa caaaaatccg gacaaattaa aatacagatt
     5761 cgtttctaaa atactaaatt catagcaaaa taaaatgccg tctgaactca ggcttc
//
LOCUS       DAWKQB010000129         5791 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.129, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000129 DAWKQB010000000
VERSION     DAWKQB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.129"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            131..565
                     /locus_tag="WJL14_001710"
     CDS             131..565
                     /locus_tag="WJL14_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ7656755.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(578..1441)
                     /locus_tag="WJL14_001711"
     CDS             complement(578..1441)
                     /locus_tag="WJL14_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ7656756.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLARLAGVGRQTELLVRNTEQFIAGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1449..2066)
                     /locus_tag="WJL14_001712"
     CDS             complement(1449..2066)
                     /locus_tag="WJL14_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ7656757.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGYNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDSRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2229..2843)
                     /locus_tag="WJL14_001713"
     CDS             complement(2229..2843)
                     /locus_tag="WJL14_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ7656758.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQTDLQEG"
     gene            3049..4821
                     /locus_tag="WJL14_001714"
     CDS             3049..4821
                     /locus_tag="WJL14_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ7656759.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFSRFTQQTGTTVETAWENGTHLPTQDEQLQMIFILQESLSNIR
                     KHAHATHIKFRLLKQDGSFTMTIQDNGQGFDTENIGEPSGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLTGASEESLK"
     gene            4818..5474
                     /locus_tag="WJL14_001715"
     CDS             4818..5474
                     /locus_tag="WJL14_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ7656760.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1434 a         1621 c         1343 g         1393 t
ORIGIN      
        1 ttccgattcc gccgttatgt ctgcctgccg gacatacgta cgcattatta acaaaagtta
       61 accgcgataa taccatcttt cacacgtcaa tctagtatat ttcctaaaat ttccaacaag
      121 aggaaaagcc gtgccactgc ctgctccctg ccgttttgcc aaacctgccg cctctttttt
      181 aagtatggct ttgctttcct gccagctttc ccacgccgcc acggcttata tccccctgaa
      241 cgattttcaa ccgaactgcg acatacgccg gctcggactg acacagggtc agcacaatga
      301 actgcgtaaa atccgcgccg ccttcaaaat ggcgggcgac agggcgcgtt tgaaggttat
      361 gcattccgaa cacagccgcc gtcggtctgt cgtcgaaatc atttcctcgg atgtttttaa
      421 tcggaacgag gcgcgcgatt atgtcgaaag ccgctatttg tccggtatgg attttgcggt
      481 ggacgaattg gaaatccaac accggttctt ccatatcctc acaccgcaac agcagcaaat
      541 gtggctttct tcctgcctca aataatcccc gaaacgctca caacgcccgt tgtttcggca
      601 gcctgcccgc ccagtcgcag gcaaactgcc acgcggaacg tcccgaacgg ctgccccgcg
      661 tctgcgccca ctgcaatgcc gccatctgtg cggtttcatc atacggcacg ccgaaatctt
      721 ccagccaatt ttgcaccgcc gccagataat cgttttgatc gaacggataa aaactgagcc
      781 acaatccgaa tcggtcggac agggatattt tttcttccac cgcttctttc tgatggattt
      841 ccccccgcat ccccgtcgta ccggcattct cgtcaaaata ttcgggcatc aggtgccgtc
      901 tgttggaagt cgcgtaaacc aaaacgttgg cgcaacgttg agacagaccg ccgtctaacg
      961 cggttttcaa tgccttatag gtttcatcgc cgctttcaaa cgacaaatcg tcgcaaaata
     1021 cgataaattt ttccggacgt tctttcaaaa gcgtcaacag gtaaggcagg ccgattaaat
     1081 cgcttttatc gacttcgatc aggcgcaatc ccttatccgc atattcgtgc agcagggctt
     1141 tgaccagcga ggatttgcct gttccgcgcg cgccgctcat caatacgtta ttcgcgggtc
     1201 tgccggcaat gaattgttcg gtattacgca ccagcaattc ggtttgcctg ccaactcccg
     1261 ccagccttgc caaggggaag gtgtgcggat cgggcaagtg ttccaaaaaa ccttttttgc
     1321 ccgcactctg ccagcggaag gcaagcgcgt tccaatccgt atgcccgggt tcgggcggaa
     1381 gcacggcatc caaacgccgc aaaacggcat aggctttatc gagaaattcg ttcaattcca
     1441 tctctacctc actttgcata tctttgcgcc atcagccgtt cgacggtatc gacgattgcc
     1501 tgcgtattcg gatcgatttc gatgttgatt ctgtcgccga cctttctgct gccgaacagc
     1561 gtccgttcca aagtttcggg aatcaaatgg acattgaaac ggctgtcttc gactttgcct
     1621 atggtcaggc tgcaaccgtc caagccgacg aaccctttgg tcaggatata gggtttgagt
     1681 tcgtgcggga gcgaaaacca aaccgtgcgg ttgtacccgt cccgttcgat ttcgacaata
     1741 ggcacggttg ccataatgtg tccgctcatg acgtgtccgc cgatttcgtc tccgaaacgc
     1801 gccgcccgtt cgatgttgac gcaatcgcct tccttcagca gccctaaatt ggtttttgcc
     1861 aaagtttccg ccattaaatc gaaactgacg cggtttcctt cgatttcggt aatcgtcagg
     1921 cagcagccgt tgttggcgac cgatgcgccg cgttgcagat tgtccgccgc ctcttgcgga
     1981 agctcgacga cataagtctg aaatgcctcc gacgggcggt ggattgccgt cagttttccc
     2041 aatccttgaa caatgcctgt aaacataatc ctgtttccct gtgtcggtaa aaatggtgca
     2101 aattgtagca tctccccgcg aaaaatgccg tctgaaatgc cttcagacag cattatgcct
     2161 ccgattcggg caaaaaccgc ccggtacggc ttgacctttc ctttccacgc cggtcggcgg
     2221 tcttaccctt atccctcctg caaatcagtt tgcgtgttca agtcggcaaa atgcccgtca
     2281 aactcgaatc tgaccggtcg cgccctttgc tgctgcaacc agcttcttaa tgttttcata
     2341 cccgaaaaca gatagggaat cgcgctttgc agaatctgcg ggcggatata cataatgttg
     2401 tagtgcatcg ttatcggcgt ttccacataa tacgcattgc acaacggtgt gcgtttcgac
     2461 acggtttcaa acctcgccac caaatcgtcc ggcagatacg gcatatcgca cggcacaacc
     2521 aaaagccagt cggcagtcgc caactgcaaa tcgttggctg cggtacacaa tgccgaaagc
     2581 gggccgaaat gctgccactg ccgtgcgtcc ggaaaaatat gcggacttct tcgagcatat
     2641 tcttccaaat tccggttggt gctgatggcg atatggctga cctgcggcct gaccctgtcg
     2701 atgacatgat ctatcagtgc cttacccccc aaaagagcaa gccccttgtc ctcgcctccc
     2761 atacggctcg cctgaccgtc ggccagtatc agggcaaaag ttttcattgc ggatgttctc
     2821 ttggaaaagt tcgaggtttt catgattgca gtctgccgtt cccaatgact caaaatgccg
     2881 tctgaagcag acggcaaata aattcatatt atctgaattt tatcataaca tgatttaaca
     2941 ctgaagcggt gcggattaag ttttccttaa ccaattcttt ctgagcagtt gtatctaatt
     3001 ccaaagaatg atattgtttg cattatttgg aacaattttt cgccgagcat gatactgcca
     3061 gcccgttttt cagacggcat cagcctttcc ctgcgcctga aactcctgac cggactgtgg
     3121 gtcgggttgg cggcattgtc tgtcgttttg acactgctgc tctctttgcg tctggaaaac
     3181 gcggcctccg tcatcgaaga ggcgggcaac ttgagaatgc aggcataccg tctggcatac
     3241 atggcgggtg aaggctcgcc ccgtgcgcaa attgacaatc aggttgccga atttgaaaaa
     3301 agtttaaaac gcattgccca aagcgatgcc atccatccgc tgattccttc ggacacccct
     3361 cttgcttatg atttgataca atccatgctg attatagatt ggcaggcaca catcctcccc
     3421 ccgctccagt cctaccggcg accgactcag gtcgatctct accgctttgc cggaaacatc
     3481 gaactgtttt tgcaggcatt ggaaaatgcc aacgaaaaaa acacatggtg gctcaggcgt
     3541 tttcaatggg caattatgtt gatgacgctg gtgtcgtctg tactgatgct gttttggcac
     3601 cagatttggg ttatccggcc gctgcaggcg ttaagggaag gtgcggaacg catcggacgg
     3661 aggtgtttcg atattccggt tcccgaaggc ggtacgccgg aattcaaaca ggtcgggcgt
     3721 tgtttcaatc aaatgggcgg caggttgaaa attttatatg atgatttgga aggacaagtc
     3781 gccgagcaga cacgcagtct cgaaaaacaa aatcaaaacc tgaccctgct gtaccaaact
     3841 acacgggatc tgcaccaatc ctacataccg caacaggctg cagaacattt tctaaaccgt
     3901 atcctgcccg ccgtaggagc agattccggc agagtttgtt tggacggcgg atccgatgtt
     3961 tatgtttcca ttcatcatgc ggattgcggc acagcagctt cggatttggg gaagtaccat
     4021 gaggaaatct tccccattga gtaccagaac gaaacattgg gcaggctgtt gctcagcttt
     4081 ccaaacggca tttctcttga tgaagacgac cgcatcctgc ttcaaacact aggcaggcaa
     4141 ttgggcgtat cgcttgccgg cgcaaaacag gaggaagaaa aacgcctgct tgcagtattg
     4201 caggaacgca acctgattgc gcaaggatta catgacagca tcgcacaagc attaacgttc
     4261 ctaaacctac aggtacagat gctggaaacc gcctttgccg aaaacaaacg ggaggaagcc
     4321 gcagaaaaca tcggcttcat caaaacaggc gtgcaggaat gttatgaaga tgtccgcgaa
     4381 ctgctgctca acttccgtac caaaatcagt aataaagaat ttcccgaagc cgttgccgac
     4441 ctattctcgc gctttacgca acagaccggc acgactgtcg aaaccgcttg ggaaaacggc
     4501 acgcacctgc ctacacagga cgagcagctc caaatgattt tcatcctgca agaaagcttg
     4561 tccaacatcc gaaaacatgc ccacgccacc catatcaaat tcagactgct caaacaggat
     4621 ggaagtttta caatgaccat tcaagacaac ggacagggtt ttgacacgga aaacattgga
     4681 gaaccatcgg gcagccatgt cggactgcat atcatgcagg agcgggccaa acgtatccgc
     4741 gccgtattga aaatctattc ccaaccccaa cagggaacca ccgtctcatt gacgggtgca
     4801 tctgaagaaa gcttgaaatg actattaaaa ttatcctgat agacgaccac accctcttcc
     4861 gcagcggcat taaagccctt ttgtcgcgcc aacacggttt tgaagtcatc ggcgaagccg
     4921 cagacggcct ctcgggcatt aaaatgatca accggctgca acccgatgtc gtcctgcttg
     4981 accttgatat gcccggtatg aacggacgcg aagcactctc ccaaatcatc agcatcaatc
     5041 cgcagcaggc agtgattatg ctgaccgttt ccgaagacaa cgacgacttg accgaatgta
     5101 tgcgcatcgg cgcgcgcggc tacctgctga aaaacatcaa cgccgacttt ctgctcgaaa
     5161 gcatacgcaa ggccgccgaa ggcgataatg tattctcgcc cgagatgacc gccaaactcg
     5221 ttaaaagcct gatttccccc caacctgccc aagggacaca ggcactctcc tcacttaccc
     5281 ctcgcgaact ggaaatcttg ggctatctcg ccgcaggaca cagcaacaaa atcatcgccc
     5341 gccacctcga tcttgccgaa tccaccgtca aagtccacgt tcaaaacctg ctccgcaaac
     5401 tcaacctcag cagccgggtg caggccgctg tttacgccat ccggcacaac gtcccccaac
     5461 ccgtgctgga ataaggcggg ttcagacggc atattagggg ttttaatccc cgtacggtca
     5521 ttcggataac agaccaagca tgtaagttta tgcccccata agtacgcttg gcatagcagt
     5581 aatattgttc ggttcagtgt tttccgtttg cccctatctg atactgcaat atcagctatg
     5641 ccgtctgaaa acgcatcatc atgatatttt cagacggcat aataaaaagc ggaaatacta
     5701 atgcagggta aaatgttcca ttccgaatcc cataaatata caatggctta tatcgtttaa
     5761 gcatgtgtaa cccaccctca tatcaatcaa t
//
LOCUS       DAWKQB010000130         5660 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.130, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000130 DAWKQB010000000
VERSION     DAWKQB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.130"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            99..815
                     /locus_tag="WJL14_001716"
     CDS             99..815
                     /locus_tag="WJL14_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ7656761.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            964..1866
                     /locus_tag="WJL14_001717"
     CDS             964..1866
                     /locus_tag="WJL14_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ7656762.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1909..2682
                     /locus_tag="WJL14_001718"
     CDS             1909..2682
                     /locus_tag="WJL14_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ7656763.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TMEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2794..4482
                     /locus_tag="WJL14_001719"
     CDS             2794..4482
                     /locus_tag="WJL14_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ7656764.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSVEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTIERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTSVFNRTVTLKDSWQPK"
     gene            4575..5531
                     /gene="gshB"
                     /locus_tag="WJL14_001720"
     CDS             4575..5531
                     /gene="gshB"
                     /locus_tag="WJL14_001720"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ7656765.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1309 a         1539 c         1568 g         1244 t
ORIGIN      
        1 aaatttgtgc aatgctttaa tttccccgac acgatttgaa acacagccgc cttaaaacat
       61 cttaagaaat ttgaaagcgt aaaaatacgg aaaacatcat gaactttgaa aacgacgaca
      121 ttatccatgc gccgaccacg tcttccctga ttctcgaaga gcggcacgat tcggagctgt
      181 tccgtgttta cgccctgatt ttggacggca ttaccgatca ggtgctgctg cctggcaaaa
      241 agctgaccga atccgaactt tgccgccaga tggtgtgttc gcgcaacacc gtccgcggcg
      301 cgctgtcgct tttggcgcac gacaagattg tcgatttgca acccaacagg ggcgcgttcg
      361 tccacgttcc cgatttgaaa gaaatgcagg atgtgttcaa tgcgcgcatc gaaatggaga
      421 cgatgatttt gaatatcctc gcagatttgc cggatttgga aacgcgcctc aagccgcttt
      481 atgcgatgat acggcgcgaa gaagaggcct ccggcagggg agaccgcgtt ggctggaacc
      541 gcctgtccaa tgcctttcac gtcgaactgg cgcgcctggt gggcaacgat gtgctgttcg
      601 acattatgaa cacgctgtgc gcgcggtctt ccctgattgt cgccgtggcg ggcgtgcatc
      661 gcgaggaaaa acacgccatc aatacgcata cgcattccga acaccgcgaa atcctcgacc
      721 tgctgctggc gggcaggcgc aaccgcgtgg tcaaaatcct gcgccgccat ttgggcaact
      781 gtatggagcg tttggaaaag actttggaag attgaatgcc ggagcggata agccgcctga
      841 accttcaggc ggttttttaa tggcgaacct gatgccgtct gaaatatgga tgcgggtatc
      901 tgcaattttc agacggcaat ttttaaaccg catatatcat gcggcaataa aggaggtcag
      961 gagatggaca gcctgatgac gttgctttcg gtattgatac cgatgtttgc cggatttttt
     1021 atccgtgtgc ccaagcctta cctgcccgct ttggataagg tgctatcggt cttggtgtat
     1081 gctgtgctgc tgctgatcgg cgtctcgttg tcgcgcgtgg aggatttggg ttcgcggttg
     1141 gacgatatgg cgttgacggt tctgtggctg tttgtttgta cggtcggggc gaacctgctt
     1201 gctttggcag tgttgggaaa gttattcccg tggcggataa aggggaaagg gaagggcgtt
     1261 tcggtcggtg tgtcgggcag tgtggggcag ctcggatgcg tgctgctcgg atttgcatcc
     1321 ggcaaactga tgcgcgatat ttggatgccg tctgaaaacg cgggtatgta ttgtctgatg
     1381 ctgctggtgc tcctcatcgg cgtacagctc aaaagcagcg gcgtatcgtt gcggcaggtt
     1441 ttggtcaacc gcaggggtat tcggttgtcg gtctggttta tgctttcatc tctttcaggc
     1501 gggctgctgt ttgccgcatc ggcagacggt gtgtcgtggg tgaaaggttt ggcgatggct
     1561 tccggcttcg gttggtattc cctctcgggt ttggtgatga ccgaggctta cggcgcggta
     1621 tggggcagta tcgcgctttt gaacgatttg gcacgagagc tgttcgcgct ggcatttatt
     1681 ccgctgctga tgaagcgttt tcccgatgcg gcagtggggg tcggcggcgc gaccagtatg
     1741 gatttcacat tgcccgtgat tcggggtgcg ggcggcttgg aagccgtacc ggtagcggtc
     1801 agcttcggcg tggtggtcaa tatcgccgct ccgtttctga tggtggtgtt ttccgctttg
     1861 ggctgaacgc ggtaaaatcg gcatcccgat gcaaggaggc agaaaacgat gaaaccgaaa
     1921 atccaaaggc acggagagat tttaagcctt gtccgccggc atcagtttat gtcggtggac
     1981 gagcttgccg ccgcattgga cgttaccccg cagacgatac gccgcgacat ccgcgaattg
     2041 gaggatgctg gcagcctgaa acgccaccac ggcggcgcat cctcgggcgg aaacttgccg
     2101 gaggggctgc ctgccagccg ccaaattcaa tgtcaaaacg aaaaaaacgc cattgcccgg
     2161 ctgattgcgg aacatattcc cgacgagtca tcgctgtttg tcagtatcgg cacgacgatg
     2221 gaagccgtgg catcggagct ggtgagacgg cgcggcagcc tgcggattat taccaacaat
     2281 atccacgtcg cctccatcgt ttcggcgcgg acggattaca cggtcatcat cacttccgga
     2341 gtcgtccgcc ctttggacgg cggcattacc ggcgtggcaa ccgtcgattt tatcaaccag
     2401 ttcaaagtcg attatgccgt gatgagtacg cacggcgtgg aaagcgacgg ttcgcttttg
     2461 gacgacgact acaaagaagt cagcgtgatg caggcgatga ttgccaacgc gcgcgtccgc
     2521 ttcctcggcg tggaccacag taaattccgc agcaacgcgc tggtcaggct cggcgacatt
     2581 acggcgtttg acaaagtatt taccgaccgg ctgcccgata ccgcgatgca gaagatgctg
     2641 aaagaggcgg gggtggaatg cctgattgcc gatgccgtct gaacgctatg tcaaagcgcg
     2701 caagtcgggt acaataaaca catcatcaaa ccgcttcaga cggcataagg aaccgcccca
     2761 atgccgtctg aagccatctg ttcaaagaaa actatgctca ataaagacca atttgcggac
     2821 aaccatttta tccgcaccat catcgaagac gacctcaaaa gcggcaaaca cgaagccgtc
     2881 caaacccgtt ttccgccgga gcccaacggt tacctgcaca tcggacacgc caaatccatc
     2941 tgcctgaact tcggtttggc gtatatttac gacggcttgt gcaacctgcg ttttgacgat
     3001 accaaccccg aaaaagaaaa cgacgaatac gtcaacgcca tcaaagaaga tgtcgagtgg
     3061 ctcggtttcc attgggcggg cgagccgcgt ttcgcttcca actatttcga ccagctttat
     3121 gactacgccg tcggtttgat taaagacggc aaagcgtatg tcgatgattt gacgcccgaa
     3181 gaaatgcgcg aataccgcgg tacgctgacc gaagcgggta aaaacagccc ttaccgcgac
     3241 cgcagcgtcg aagaaaacct cgacctgttc acacgcatga aaaacggcga gttccccgac
     3301 ggcagcaaaa ccctgcgcct gaaaatcgac atggcatcgg gcaacatcaa tatgcgcgac
     3361 cccgtcatct accgcatccg ccgcgcccat caccacaaca ccggcgacaa atggtgtatc
     3421 tacccgatgt acgactacac gcattgcatc tccgatgcca tcgaaggcat cacgcattcc
     3481 ttgtgtacgc tcgaatttga agcacaccgt ccgctgtacg actgggtgtt ggataacatc
     3541 cacgcgccgc atgccacccg tccgcgccaa tacgagtttt cccgtttgga gcttttgtac
     3601 accattacct ccaaacggaa attgaatcag ttggttgtgg aaaaacacgt ttccggctgg
     3661 gacgatccgc gtatgccgac tatttccggt atgcgccgac gcggctacac gcccgaaggc
     3721 ctgcgtctgt ttgccaaacg cgccggtatt tccaaatctg aaaacatcgt cgatatgagc
     3781 gtgttggaag gcgcgattcg cgaagagttg gaaaattccg ccccacgcct gatggcggtg
     3841 ttgaacccgc tcaaagtgac cctgaccaac tttgaagccg gcagaaccca aagccgccgt
     3901 gccgcgttcc atccgaacca cgaggaaatg ggcgagcgcg aagtacctat ttcacaaacc
     3961 atctatattg aagccgacga ctttgccgaa aatccgccta aaggcttcaa acgcctgatt
     4021 cccggcggcg aagtacgctt gcgccacggc tatgtcatca agtgcgatga agtcgtcaaa
     4081 gacgaagcag gcaatgtggt tgaactcaaa tgcagcatcg accacgacac cttgggcaaa
     4141 aatccagaag gccgtaaagt taaaggcgtg attcattggg tttccgccga acacgccgcc
     4201 gaaatcaaag tccgtctgta cgaccgcctc tttaccatcg agcgtcccga tgccgtgcgc
     4261 ggcgaagacg gcgaatacct gccgttcacc gatttcctca atccggaatc cgtcaaggaa
     4321 atcaccgcct acgccgaacc tgccgccaaa gatttgccgg cagaaagccg ttggcagttc
     4381 gagcgtatcg gctacttcgt aaccgaccgt aaagaccacg gcaaagacac gtcggtgttt
     4441 aaccgcacgg tcacgctgaa agattcttgg cagcctaagt aaaaccccat ccttgccgtc
     4501 tgaatattgt tcggacggca tttccccttt gcccgcgaaa tgcggcacat tcggcacacc
     4561 ggaaaggaaa tatgatgaaa gtcctcttta tcgccgaccc gatggcaagt ttcaaaacct
     4621 acaaagacac cacctacgcc atgatgcgcg agatggcaaa acgcggttgg cggctgtttc
     4681 ataccttgag cggggaattg tcggttaaaa acggtctggt ggttgctgat gcgtctgctt
     4741 ttgagtttct cggcgcacaa gacgaccacg acaaaaactg gtttgccgcc gccgacaaag
     4801 ttcagacggc attgaaagca tttgatgccg tgattatgcg taccgatccg ccgttcgata
     4861 tgcaatacct ctacgccacc caattactga cgctggcgga acagcagggc gcgaaagtct
     4921 ttaacagcgg acagtcgatg cgtgacttca atgaaaaact ggcgattttg aacttcagcc
     4981 gctttaccgc gcccacgctg gtaacgaccc gttccgccga tgtccgcgca tttttgaaag
     5041 aacacggcga cattatcatc aaaccgctgg acggtatggg cggtatgggt attttccgcc
     5101 tgaccgaaaa agaccccaac atcggcagca tcctcgaaac cctgatgcgg cttgattccc
     5161 gcaccattat ggcgcaacgc tacattcccg aaatcgtaca cggtgacaaa cgcatcctga
     5221 ttatcggcgg cgaagtcgtc ccctatgctt tggcgcgtat cccgcaaaac ggcgaaacac
     5281 gcggcaacct tgcggcaggc ggacgcggcg tggcgcagga attggacgga cgcgaccgcg
     5341 aaattgccga aacgctcgcc cccgaactca aacggcgcgg catcctgctg gccggtttgg
     5401 acgttatcgg cagcaacctg accgaagtca acgtaaccag cccgaccgga ttccaagaaa
     5461 ttatgaaaca aaaaggtttc gacgtggcgg caatgtttgc cgatgccgtt gccgcgtggt
     5521 cggtacgtta aaccgatgcc gtttgaaagg cttttgcttc acaaccgttt ggtttgttcg
     5581 ggcaggcagg gttttccctc agccggcagg gatgcggtta atgccgtacg gacaccccgc
     5641 cgccccgttt tcagacggta
//
LOCUS       DAWKQB010000131         5658 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.131, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000131 DAWKQB010000000
VERSION     DAWKQB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.131"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(554..3295)
                     /gene="tbpA"
                     /locus_tag="WJL14_001721"
     CDS             complement(554..3295)
                     /gene="tbpA"
                     /locus_tag="WJL14_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ7656766.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDTNQYAHFVVEEECKNRNYEACKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLSDPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDENRKYGPIRGNGKYAGNHKYGGLFTNGENGALVGAEY
                     GTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADGS
                     DKNCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGSN
                     LRHQDYYFQNAKRAYSLKTPPKNGNQNSPNGDKSKPYWVSIGGGNVVTEQICRLGNNT
                     YTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTLSW
                     NAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGIVF
                     KGDFGNLEASWFNNAYRDLIVRGYEAQIQNGKEEAKGDPAYLNAQSARITGINVLGKI
                     DWNGVWDKLPEGWYSTFAYNRVHVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYDQP
                     EGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTIKK
                     HFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3382..5484)
                     /locus_tag="WJL14_001722"
     CDS             complement(3382..5484)
                     /locus_tag="WJL14_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7656767.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRFKRRNWHPRVNPKEDKIELSENDWEQTDNGNIKTPS
                     KQKNIINALLGSEGALLQDSSQEGQSISKVTDHHNFQYVWSGFFYKHIQTKFETTGGK
                     MTARSGPDGYIFYKGKDPSRKLPVSGEVMYKGTWDFLTDVKANQKFTDLGNASTKPGD
                     RYSAFSGELDYIVKQENDKKDGHVGLGLTTEITVDFEKKTLSGKLIKNNSVITTNNDK
                     HTTQYYSLEATLKGNRFNGKATATDKPKENEIKQHPFVSDSSSLSGGFFGPQGEELGF
                     RFLSDDKKVAVVGSAKTKDKAESGNGNGNGTSGGASVSASNGAAGTSSENSKLTTVLD
                     AVELKSGDKEVKKLDNFSNAAQLVVDGIMIPLLPEASESGSNQANQGTNGGTAFTRKF
                     NHTPKSDEKDTQAGTAENGNPAASNTAGDANGKTKTYEVEVCCSNLNYLKYGMLTRKN
                     SKSAMQAGESSSQADAKTEQVEQSMFLQGERTDEKEIPNDQNVVYRGSWYGHIANGTS
                     WSGNASDKEGGNRADFTVNFGTKKINGTLTADNRQAATFTIVGDIEGNGFSGTAKTAD
                     SGFDLDQSNNTRTPKAYITNAKVQGGFYGPKAEELGGWFAYSDDKQTKNATDASGNGN
                     SASSATVVFGAKRQKPVQ"
BASE COUNT         1213 a         1436 c         1388 g         1621 t
ORIGIN      
        1 ccgataaatt gccttagcat tgaatgtctg gattcccgcc tgcgcgggaa tgacgggatt
       61 tgagattaca gtgtatcgag aaggatggga aacggtggga attgtgtaaa aaatgccgtc
      121 tgaagccttt cagacggcat ttgcggcatt cggacattta aaaaccccat cattcccgcg
      181 caggagggaa tccgaataca tccgaacaga aacctgcatc ccgtcattcc cgcgaaagcg
      241 ggaatctaga acgtaaaatc taaagaaacc gttttatccg ataagtttcc gcaccgacag
      301 gtctagattc ccgcctgcgc gggaatgacg ggatttgagg tttctgtttt tgattttctg
      361 ttttcgcggg aatgacggtt tagaagttac ccgaaacctc aaaaaaacct aaaccgaacg
      421 gactggattc ccgcctgcgc gggaatgacg aaatttcagg attacggtgt atcggggatg
      481 atgggaaacg gtgggaattg tgtaaaaaat gccgtctgaa gcctttcaga cggcatttgc
      541 ggcgtttgga cgtttaaaac ttcatttcca agctaaatgt gtagttgcga ccgggggcgg
      601 catatcggtt gtaaacgccg acatttttgt gttgattgac tgcgccgccg gcagtttgcc
      661 gcacattttc ccaagtaaca tagcggtagt tgaggaggtt gtacacgccc gcacggaggg
      721 tgaagtgttt tttaatcgtg taataaccgg acacatccac aatataccaa gggcgggtac
      781 ggcgcgcggt ggcttttgta ttgcggctgt tgccgttgag caaagcccgg ctgcccaaca
      841 actctgtgat ttccttggct ttggaataag tcagcatacc gttcacaccc catttgcctt
      901 ccggttggtc atagcccaag ccgacgacat agcgcgaggg ttggatggca tcaaacaggt
      961 gtgattgaat atcggtgcgg tctgcgcgtt ttttgatgtc gcggacatgg acacgattat
     1021 aggcaaatgt agaataccaa ccttcgggca atttatccca tacgccgttc caatcgattt
     1081 tgcccaaaac attgatgccg gtaatccgcg cgctttgggc attgaggtaa gccgggtcgc
     1141 ctttggcttc ttctttgccg ttttgaattt gcgcttcata accccggaca atcaaatcgc
     1201 ggtaggcatt gttgaaccaa cttgcctcca agttgccgaa atcgccttta aacacgatgc
     1261 cggcttcttt gttgaacgat ttttccggat cgattttgac cgccttgctt tgaacacccg
     1321 cccgccagcc gtacatttcc gcaaacgagg gcaggcggaa gccggttgaa gtgcggtaag
     1381 tcaaatccag ccagtcggca ggtttgagaa cgatgccggc gttccaggac agggtgcggt
     1441 gcgtgccggt ggaaacgctg ccgtcgtccg aatgcgtgct gcggtagtcg tagcgcaagc
     1501 ccgcgccgac atccgcccac ctgcccaaac ggacattgtc ccgaactgcc gcgtaatagc
     1561 ttttgccgtt gatgctgcgc ggcgtgcagt ccgtataagt attgttgccc aagcggcaga
     1621 tttgctccgt aaccacattt cccccgccta tgctgaccca atagggtttg ctcttgtcgc
     1681 cgttggggct gttttgattg ccgtttttag ggggcgtttt caacgaatag gcgcgtttgg
     1741 cgttttgaaa ataataatcc tgatggcgga gattagagcc aaagcggtca aacccgagat
     1801 tcacgctcag gttgtggcgg attttggcgg tatcgaagga ttttttgaat gccgcctgca
     1861 agagcctgtg gctttccccg taaatcacgc ggtcggattt gtaataggaa aacggcttgt
     1921 cggcactcgg acggcaattt ttgtccgaac cgtcggcaga acagtgcgtc tgctgaaaat
     1981 gattgtccaa accgataccc tgccggtcat aagagaggcg ggcataatcc gcccaagtgt
     2041 ctttatcggc attggtatag acatattcca aaccgtagcg gcttttggtg tgcgtctcgt
     2101 cgtaaaacac gcctgtaccg tattccgcgc ccaccagcgc accgttttcg ccgttggtaa
     2161 acagtccgcc gtatttgtgg ttgcccgcat atttgccgtt gcctcttatc ggtccgtatt
     2221 ttctattttc atcaaaaacc gccttggtca ggaatgccgg aaccgtcata tcgcgcgtgt
     2281 cgaaagtttg ttgcgtgtgt tcgagtatgc cgccgatgta gtgccgtttg ttttcaaaac
     2341 gaaaacccgg gcggaacagc cacgaccggc tttcgtatga aagcggatcg gagaggaagc
     2401 ggttggggcc cgtgtagtct cgggtggaaa ccgtttgacg ttcgtctttg ccgacaacat
     2461 cttttttcgg attcgcttta cacgcttcgt aattcctgtt tttacattct tcttcgacca
     2521 cgaaatgagc gtactgattg gtatcctcaa ccggcaccag cctgttaaag ctctgaacgc
     2581 cgcgtccggc atcttcgtgg gcgcggattt cccccgcgcg ccgcccggtg tggatcagca
     2641 aagcctccgc accgccgatg cgccccgcca gcgcgatgga ttgggtaagc ccccggtttt
     2701 tgccggaata ggcggtttta ctctgaatgc cccactgcct gccttccccg ataacatcgt
     2761 cggcggtttt ggtttgaaat gcgaccgagc ccgccaatgc gccgctgcct tgttcgaccg
     2821 agtttgagcc tttgctgatt tcgacagctt tgacgttttc atactcgatt tcattgattg
     2881 cgccgctgct gcccgccgtc ctcgtcccgc ccaatgccgc ctgcgcggtg taggactgta
     2941 tttgcgccaa gccgtccacc gtcaaggaaa cgcggttttt gtccatgccg cgtatcgagt
     3001 agcccgaact tgcgccgcgc ccctgttcga ccacggcgat gccggggtcg taacgcgtca
     3061 ggtcgcggat atcgagtacc tgttccttgc tgagggtgtc ggcggttttg accaatttgc
     3121 ccaaaccggt tacttcgtta tcgcggcggg ttttctgttt tttggctttt acctgtatgg
     3181 tatccaactg tttttcctgt gcttgtccgg cttgcacatt ttctgcataa acgggcagcg
     3241 cagtcattaa agacaggcat aaaatattta atcggaacaa atgttgctgt tgcatagtgt
     3301 ttccctaatc ttcgctttca gacggtatcg gaaggagcgg tgccgtctga ggccttattc
     3361 ttgattgttc ggcaaccgtg cttattgcac aggcttttgg cgtttcgcac cgaatacgac
     3421 agttgcactg cttgctgaat ttccattgcc ggatgcatct gttgcatttt tcgtttgttt
     3481 atcgtccgaa taggcaaacc atccgcccaa ctcttcggct ttgggcccgt aaaaaccgcc
     3541 ctgcaccttg gcgtttgtga tatatgcctt aggcgtgcgg gtgttattgc tttgatcgag
     3601 atcaaaacct gagtcagcag ttttcgccgt accggaaaaa ccgttgccct caatatcgcc
     3661 cacaatggta aaggttgccg cctgcctgtt gtcagcggtt aacgtgccgt taattttttt
     3721 cgtaccgaaa ttcacagtaa agtccgccct gttgccgccc tctttatcgg aagcattgcc
     3781 gctccagctt gtgccgttgg caatatgccc gtaccaagac ccccgataaa cgacgttttg
     3841 gtcgtttgga atctcttttt catcggtgcg ctcgccttgg aggaacatac tttgttcaac
     3901 ttgttccgtt ttagcatcag cttgactact gctttctcct gcctgcatcg cggacttgct
     3961 gtttttgcgc gtcaacattc cgtatttcag ataattgagg ttggaacagc agacttcgac
     4021 ttcataggtt tttgttttgc cattggcatc acctgccgta tttgaagcgg ctggattgcc
     4081 attctccgcc gtacctgctt gggtgtcttt ttcatcgctt ttcggcgtgt ggttaaattt
     4141 gcgggtaaag gctgttccgc catttgtacc ttgattggcc tgattgctcc cgctttcgga
     4201 agcctcgggc aggagcggaa tcataatgcc gtcgacaacc agttgggcgg cattgctgaa
     4261 gttgtcgagc tttttgactt ccttatcgcc cgatttcagc tcgaccgcat ccaaaaccgt
     4321 ggtcagctta ctgttttcag acgacgtgcc tgccgcaccg tttgatgcgg aaacgcttgc
     4381 accgcccgaa gtaccgttac cgttaccgtt accgctttcg gctttgtctt tggttttcgc
     4441 gctgccgaca acggcaactt ttttatcgtc gctcaaaaag cggaaaccca attcctcacc
     4501 ctgcgggccg aaaaagccgc cgcttaaaga agacgagtcg gaaacaaagg gatgttgttt
     4561 gatttcattc tcttttggtt tgtcggttgc cgttgccttg ccgttgaagc ggtttccctt
     4621 aagcgtcgcc tcaaggctgt aatattgcgt ggtgtgttta tcgttattag tagtgattac
     4681 actgttgttt ttaattaatt ttccgctgag ggtttttttc tcaaaatcaa ccgttatttc
     4741 cgttgttaat cccaaaccta cgtgcccgtc ttttttatca ttctcttgtt tgactatata
     4801 atccaactcc ccggaaaaag cactatatcg gtcgccgggt ttcgtagaag cattccctaa
     4861 atctgtaaat ttctgatttg ctttcacatc agttaaaaaa tcccaagtac ctttgtacat
     4921 aacctcccct gaaacaggaa gttttctcga gggatctttg cctttataaa aaatataacc
     4981 gtcaggccca cttcgggctg tcatctttcc accagttgtt tcaaatttag tttgaatatg
     5041 tttataaaaa aaacccgacc atacgtattg gaaattgtga tggtccgtaa ccttagatat
     5101 actctgacct tcttgacttg aatcttgcaa taatgctccc tcacttccaa gtaaggcatt
     5161 aataatattt ttttgtttgg aaggggtttt gatattacca ttatccgttt gttcccaatc
     5221 attttcagag agttctatct tatcttcttt aggattcacc ctcggatgcc aattccgccg
     5281 cttgaagcgc attgcaaaac cgtatccgcc ttggtctttt tgggcttgcg gcttttcgga
     5341 agaaacatct tgatattttg gcgcgggacg cggggcttcg gtatcgacag aatcaagatc
     5401 gaaactgccg cctccgccca aacaagcact caacaaaaac acaggcagca ccatagcagc
     5461 ctgatttacc aatggattgt tcatatagac ccaattcaat taaagaatga taaggattat
     5521 tattttattt atttttaaca aatttgcaaa tactttttta tttttttagg gaatacacca
     5581 aaatcccgtc attcccgcgc aggcgggaat ccgaacgcgt ccgcacggaa acctatatcc
     5641 cgtcattccc acgtatat
//
LOCUS       DAWKQB010000132         5639 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.132, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000132 DAWKQB010000000
VERSION     DAWKQB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.132"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(122..883)
                     /locus_tag="WJL14_001723"
     CDS             complement(122..883)
                     /locus_tag="WJL14_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656768.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            983..1234
                     /locus_tag="WJL14_001724"
     CDS             983..1234
                     /locus_tag="WJL14_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656769.1"
                     /translation="MSSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1286..2092
                     /locus_tag="WJL14_001725"
     CDS             1286..2092
                     /locus_tag="WJL14_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ7656770.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2399..3922
                     /locus_tag="WJL14_001726"
     CDS             2399..3922
                     /locus_tag="WJL14_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ7656771.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLDKGEELPVDFTNRLIYYVGPVDPVGDEIVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPKWQAKIGIIPVES"
     gene            4020..5432
                     /gene="trkA"
                     /locus_tag="WJL14_001727"
     CDS             4020..5432
                     /gene="trkA"
                     /locus_tag="WJL14_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ7656772.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1404 a         1714 c         1364 g         1157 t
ORIGIN      
        1 cgtcgccttg tcctgatttt tgttaatcca ctatattaat attttgcgcc tgttctatga
       61 tgctttcaag tcgggtgaga atgcaaatgc cgtctgaaac ggctttcaga cggcatggca
      121 atcagcgttt gtattttaac tcgtacttga tgtcgttgag gattttgcgg acatcgtgtt
      181 ccaacacgtc ttcgactacc gcacccgcct gctcgtgcag catctgctgg agctgatagg
      241 taaaaaccgc catctgcttt tgcaccgccg tacggatgat accgttgacg gtatcggtca
      301 gatgcgggcg caggcgtttg atcagccgtt cggtcagctc ctgttcggac aggcagaaca
      361 cttcgcgccg gttgacggct ttcgggttca gaatattgat ttggacgggc atcaacgctt
      421 cttccgcacc gccttcgccg ttttccgcaa ccccgtctgc cgccgtaccc gattctgccg
      481 catcggcgtt ttccccgcct tcaatctgtc cggtttcaaa ttcggcactg tcttttttgg
      541 catcaaaccg gattctccgc cgcgattcga tgtgtttttc cgaaaccgac atttgcaggg
      601 aagcctgcgc gttgagccag ttttcctgaa ggacgatcat cgggtcggtt tcgacttcct
      661 cgccgcaatc ggcaacggcg gcattgtgtt cctcctgcca ttttttcaga tacgccttca
      721 acacacgggc tcggctctca tcgtccagtt tcggcacagg cgcgtccgtt ccggtttcag
      781 aggggcggga cagcggcgcg taagtcggca ctgccttcat acggcgcgtc tgacgcaggt
      841 tttccaaacg tttttcccaa ttcggctctt tattcgcatc cattttcggc ttccggttct
      901 taatctttgc aggcagacaa acccgcgccc aaagcgcggt ttgatataat ggcgcatttt
      961 aacagattcg cgaggataca tcatgagcag catcgaacag cgtttggaat atctggaaga
     1021 ggcgaacgac gtgctgcgta tgcagaacca cgtcctgtcc accgccttca aagccctgat
     1081 ccgcgccctt cccgccgaca ccgccgaagt cgcggtcgag tccatccagc ttgcatttga
     1141 agacgccttg gcagaattga gctatgagga cagcccgcat acggatttgt tccacgacgt
     1201 tacttatgcg tttttccgtg aaaaagaacg ttaattttat gttaaactga ttttttaggc
     1261 tttttgatta ccgaaaggaa ttttgatgaa tatgaaaaaa tggattgccg ccgcccttgc
     1321 ctgttccgcg ctcgcgctgt ctgcctgcgg cggtcagggt aaagatgccg ccgcgcccgc
     1381 cgcaaatccc gacaaagtgt accgcgtggc ttccaacgcc gagtttgccc cctttgaatc
     1441 tttagactcg aaaggcaatg tcgaaggttt cgatgtggat ttgatgaacg cgatggcgaa
     1501 ggcgggcaat tttaaaatcg aattcaaaca ccagccgtgg gacagccttt tccccgcctt
     1561 gaacaacggc gatgcggacg ttgtgatgtc gggcgtaacc attaccgacg accgcaaaca
     1621 gtctatggac ttcagcgacc cgtattttga aatcacccaa gtcgtcctcg ttccgaaagg
     1681 caaaaaaata tcttcttccg aagatttgaa aaacatgaac aaagtcggcg tggtaaccgg
     1741 ctacacgggc gatttctccg tatccaaact cttgggcaac gacaacccga aaatcgcgcg
     1801 ctttgaaaac gttcccctga ttatcaaaga actggaaaac ggcggcttgg attccgtggt
     1861 cagcgacagc gcagtcatcg ccaattatgt gaaaaacaat ccgaccaaag ggatggactt
     1921 cgttaccctg cccgacttca ccaccgaaca ctacggcatc gcggtacgca aaggcgacga
     1981 agcaaccgtc aaaatgctga acgatgcgtt gaaaaaagta cgcgaaagcg gcgaatacga
     2041 caaaatctac gccaaatatt ttgcaaaaga agacggacag gccgcaaaat aagcccgccc
     2101 atccgaacac aatgccgtct gaagcccttt cagacggcat tgttcatcaa tcggcctaca
     2161 atgaactgcc tgctgatttc tccctaccgc aaagcaacag gcaaagatta caaatatcaa
     2221 aatccgagta aaacagtatt ttattaaaac aaattgataa tcaagagatt agaattatgt
     2281 attgtcttta ccgtacaaac gctggcacta tttcaacctg ataaaaaaca gccttcaaaa
     2341 aggttgttta aaacagcagc agacacttac cgccacaacc ttgaaaagga acacaatcat
     2401 gaccgtcatc aaacaggaag actttatcca aagcatttgc gatgccttcc aattcatcag
     2461 ctactaccat cccaaagact acatcgacgc gctttataag gcgtggcaga aggaagaaaa
     2521 ccccgccgcc aaagacgcga tgacgcagat tttggtcaac agccgcatgt gtgccgaaaa
     2581 caaccgcccc atctgccaag ataccggtat cgcgaccgtg tttttgaaag tcggtatgga
     2641 tgtgcaatgg gatgcagaca tgagcgtcga agagatggtt aacgaaggcg tgcgccgcgc
     2701 ctacacttgg gaaggcaata cgctgcgcgc ttccgttctc gccgaccccg ccggcaaacg
     2761 ccaaaatacc aaagacaaca cgcccgccgt catccatatg agcatcgtgc cgggcgacaa
     2821 agtcgaagta acctgcgcgg caaaaggcgg cggttctgaa aacaaatcca aactcgccat
     2881 gctcaaccct tccgacaaca tcgtcgattg ggtattgaaa accattccga ccatgggcgc
     2941 gggctggtgt cctcccggca tcttgggcat cggcatcggc ggtacgcccg aaaaagccgt
     3001 gttgatggcg aaagaatccc tgatgagcca catcgacatc caagaattgc aggaaaaagc
     3061 cgcgtccggc gcggaattgt ccaccaccga agccctgcgc ctcgaactct ttgaaaaagt
     3121 caacgcgcta ggcatcggcg cgcaaggctt gggcggtctg accaccgtgt tggacgtgaa
     3181 aatcctcgat tacccgaccc acgccgcctc caaaccgatt gccatgattc cgaactgcgc
     3241 cgccacccgc cacgtcgaat ttgaattgga cggctcaggc cctgtcgaac tcacgccgcc
     3301 gcgcgtcgaa gactggcccg atttgactta cagccccgac aacggcaaac gcgtcgatgt
     3361 cgacaagctg accaaagaag aagtggcaag ctggaaaacc ggcgacgtat tgctgttgaa
     3421 cggcaaaatc ctcaccggcc gcgatgccgc acacaaacgc ctcgtcgata tgctcgacaa
     3481 aggcgaagaa ttgcccgtcg atttcaccaa ccgcctgatt tactacgtcg gccccgtcga
     3541 tccggtcggc gacgaaatcg tcggcccagc aggtccgacc accgccaccc gcatggacaa
     3601 attcacccgc caaatgctcg aacaaaccga cctcttgggc atgatcggca aatccgagcg
     3661 cggcgcggcc acctgcgaag ccatcgccga caacaaagcc gtgtacctca tggcagtcgg
     3721 cggcgcggcg tatctcgtgg caaaagccat caaatcttcc aaagtcttgg cgttccccga
     3781 attgggcatg gaagccattt acgaatttga agtcaaagac atgcccgtaa ccgtcgccgt
     3841 agacagcaaa ggcgaatcca tccacgccac cgccccgccc aaatggcagg cgaaaatcgg
     3901 catcatccct gtcgaatctt gaggcgccat gccgtctgaa cacaaaatct gccttcagac
     3961 ggcatttccg cccccggttg cggtacaatc caccatttca tcactcggcg acccacaccg
     4021 tgaaaatcct cattttaggc aacggacagg taggttctac cgtcgcacaa aaccttgccg
     4081 ccatacccaa caacgacgta accgttatcg acatcgacga aaaagcattg caggaaacag
     4141 gcagccgcct cgatgtccaa accgttttcg gcaacggcgc atcccccttc acattagaac
     4201 gcgccggcgc ggaagatgcc gacttgctgc tcgcgctctc ccgcagcgac gaaaccaaca
     4261 tcgtcgcctg caaagttgcc gccgacctgt tcaacatccc cggccgcatc gcgcgcgtcc
     4321 gttccagcga atacctcgaa tacctcagcc ccaagctcga aaacaacgaa aacggcagcc
     4381 tttccatatt cggcataacc gaaaccatca gccccgaaca gctcgttacc gaacagcttg
     4441 ccggcctgat agactgcccg ggcgcattgc aggttttacg ttttgcagac gaccgcgtgc
     4501 ggatggtcat catacaggcg cggcgcggcg gactgcttgt cggacgcagc attgccgaca
     4561 tcgcccaaga tttgcccgac ggggccgact gccaaatctg cgccgtttac cgcaacaacc
     4621 gcctcatcgt ccccgcgccg caaaccgtca tcatcgaagg cgacgaaatc ctgtttgccg
     4681 ccgccgccga aaacatcggc gcggtcatac ccgaattgcg ccccaaagaa accagcaccc
     4741 gccgcatcat gattgccggc ggcggcaaca tcggctaccg tctcgccaag cagctcgaac
     4801 acgcatacaa cgtcaaaatc atcgaatgcc ggccgcgccg tgccgaatgg atagccgaaa
     4861 acctcgacaa caccctcgtc ctgcaaggtt cggcaaccga cgaaaccctg ctcgacaacg
     4921 aatacatcga cgaaatcgac gtattctgcg ccctgaccaa cgacgacgaa agcaacatta
     4981 tgtccgccct tttggcgaaa aacctcggcg cgaagcgcgt catcggcatc gtcaaccgct
     5041 caagctacgt cgatttgctc gaaggcaaca aaatcgacat cgtcgtctcc ccccacctca
     5101 tcaccatcgg ctcgatactc gcccacatcc ggcgcggcga catcgttgcc gtccacccca
     5161 tccggcgcgg cacggcggaa gccatcgaag tcgtcgcaca cggcgacaaa aaaacttccg
     5221 ccatcatcgg caggcgcatc agcggcatca aatggcccga aggctgccac attgccgccg
     5281 tcgtccgcgc cggaaccggc gaaaccatta tgggacacca taccgaaacc gtcatccaag
     5341 acggcgacca catcatcttt ttcgtctcgc gccggcgcat cctgaacgaa ctggaaaaac
     5401 tcatccaagt caaaatgggc tttttcggat aaaccgcccc attccggaca cattgccgcc
     5461 aagcggtatg gaagcggaat aatggtaggt gggcttcaga cggcatcgtc ccccaccgtt
     5521 attcccgcgc aggcgggaat ccggtctgtt cggtttcagt tgtttttggg tttcgggtaa
     5581 tttccaaatc gtcattcccg cacaggcggg aatccaaacc attggacagc ggcaatatt
//
LOCUS       DAWKQB010000133         5602 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.133, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000133 DAWKQB010000000
VERSION     DAWKQB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.133"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(325..1371)
                     /gene="recA"
                     /locus_tag="WJL14_001728"
     CDS             complement(325..1371)
                     /gene="recA"
                     /locus_tag="WJL14_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ7656773.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVADEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1625..2389
                     /locus_tag="WJL14_001729"
     CDS             1625..2389
                     /locus_tag="WJL14_001729"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ7656774.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPIVTMSMGQTGTVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2409..4424
                     /gene="rep"
                     /locus_tag="WJL14_001730"
     CDS             2409..4424
                     /gene="rep"
                     /locus_tag="WJL14_001730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ7656775.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKHLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAEMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNAWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFALMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVVKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIIKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGVRIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNAYAHEHECSLYEAAQTEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4492..5550)
                     /gene="dinB"
                     /locus_tag="WJL14_001731"
     CDS             complement(4492..5550)
                     /gene="dinB"
                     /locus_tag="WJL14_001731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ7656776.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCTALLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         1294 a         1527 c         1557 g         1224 t
ORIGIN      
        1 tgagaaaccg ttttactcga taaatttccg tgccgagggg tctggattcc cgcgttcgcg
       61 ggaatgacgg catatttttt gcatttgata taaagggtcg tttgaatttt gttcagcaag
      121 tgcaaagtgt tgcacataaa agggcgtagg cttcatccgt aaagtcggcg caggatagag
      181 gcaaagcggg cgtaggtcgg gctgtagcaa ctgtattttt caccccgtcg ggcaaaaata
      241 ccaaaactca aatcaagccg ttcggaggcg gctcaaaaaa acggtacttc gcagcagaag
      301 taccgtttgt cgggatttta agttttattc ttcggggcgt tcgccgtcgg tttcgtcctg
      361 tgtcccttcg gtgatgtgca tttctacgcc gttgagggcg cggattttcg catcgatctc
      421 atcggcaacc tcaggattct ccttcaacca gacacggacg ttgtccttgc cctgcccgat
      481 tttcgcaccg ttgtagctgt accacgcgcc ggatttgttg atgatgtcgt ttttcacgcc
      541 gatgtcgatc aattcgcctt cccaactgat gccttctccg tagaggatgt caaactctgc
      601 ctgacggaac gggggggcga ctttgttttt gatgactttg acgcgggttt cgttgcccaa
      661 tacctcttcg ccttttttga tggatccggt gcggcggatg tcgaggcgga cggaagaata
      721 gaatttcagc gcgttgccgc cggtggtggt ttcggggctg ccgaacatta cgccgatctt
      781 catccggatt tggttgatga acacaaccag cgtgttggtt tttttgatgt gtccggtcag
      841 tttgcgcaaa gcctggctca tcaggcgcgc ctgcagtccg acatggctgt cccccatatc
      901 gccttcgatt tcggctttgg ggacgagtgc ggctacggaa tcgacgacta ccatatctat
      961 gccgcccgaa cggacgagtg tgtcgcagat ttccaaagcc tgttcgccgg tatcgggctg
     1021 ggacaggtaa agctcttcga cttttacgcc gagtttgcgg gcgtaaacgg gatcaaaggc
     1081 gtgttcggca tcgacaaagg cgcacacgcc gccgtttttc tggcattggg cgacggcttc
     1141 gaggcagagg gtggttttgc cggaggattc ggggccgaag atttcgacga tgcgcccgcg
     1201 cggcagaccg ccgactccga gggcgaggtc taatccgagc gatccggtgg aaatgacttc
     1261 gaggttttct tcctgctggc tgccgtccat tttcatgatg gcgcctttgc cgaaactttt
     1321 ttcgatttgc gccagtgcgg cggcaagggc tttgcttttg tcgtctgaca ttggggttac
     1381 tccggaacaa atgcggtatg tgggatgcgg cgcaacacgg gctgcggcgc gggatgtgta
     1441 tcgttttccc gatgtgcggg ctatcggtaa tgctgcttca cggggttgcc attatcgcat
     1501 atttccttgc ttgccgatat gcggcaggac gcggcggctt gtgccggaat ggaatctgga
     1561 tgccgtctaa aaggcggccg gctttgttat aatggcggct gttttttctg tgtgtgcctg
     1621 ttttatgtgt tcctgccttg ttgtcaaaaa taccgttatc ggaagcggac gcaccaaaat
     1681 cgccgtgccg cttgtcgccc gcgatgctgc cgaactttcc gccgtacttg agcaaatcaa
     1741 aaatatgccc ttcgatattg cggagttccg cgccgacttt ttggaatgcg cgggcagcat
     1801 cggcgaagta ttgcgccaca cgcagaccgt ccgcgacgcg ctgcccgaca aaccgctgct
     1861 gtttacgttc agacggcatg gcgaaggcgg ctcgttcccg tgttcggacg attattattt
     1921 tgaactgctc gacgcgctga tcgaaagccg cctgcccgac atcatcgaca tcgagctgtt
     1981 ttccggcgaa accgccgtcc ggcgcgccgt ggcaaatgct caaaaaaacg gcatcgccgc
     2041 cctactctgc aatcatgagt ttcaccgcac gccgccgcaa gaagaaatcg tatgccgtct
     2101 gaaacagatg gaggactgcg gcgcggacat ctgcaaaatt gcggtgatgc cgcaaagcgc
     2161 ggaagatgtg ctgactttgc tttccgccac gctcgaagcg aaacggcttg ccgccaaacc
     2221 gattgttaca atgtcgatgg gacagacggg gacggtcagc cggcttgccg gacaggtgtt
     2281 cggctcaagc atcacgttcg gttcgggaac gcaaaactcc gcgccggggc aaatcggcgt
     2341 atccgccctc cgtgcggcac tcgactgtct cgaaagcggc gcagactgat ttcagacggc
     2401 atcaaaacat gatgaaactc aatccccaac agctcgaagc cgtccgttac ctcggcggcc
     2461 ccctgctcgt ccttgccggt gcaggcagcg gcaaaaccgg cgtaattact caaaaaatta
     2521 agcatttgat tgtcaatgtc ggctatctgc cgcataccgt cgccgcaatt acctttacca
     2581 acaaagccgc tgcggaaatg caggagcgcg tcgccgaaat gctgcccaag tcgcaaacgc
     2641 gcgggctgac aatttgcacg ttccattctt tgggcatgaa gattttgcgc gaagaggcga
     2701 accatatcgg ttacaaaaaa aacttttcca tcctcgattc caccgacagc gcgaaaatca
     2761 tcggcgaact tttgggcggt acgggcaaag aagccgtatt caaggcgcag caccagattt
     2821 ccttgtggaa aaacgattta aaaacgcctg aagatgtcgt tcagacggcc tctaatgcgt
     2881 gggaacaaca aaccgcacgc gtgtatgcga gctatcagga aaccttacaa agctatcagg
     2941 cagtggactt cgatgactta atccgcctgc ctgccgtgct gttgcagcaa aacagcgaag
     3001 tgcgcaacaa atggcagcgg cggctgcgtt atctgctggt tgacgaatgc caagatacga
     3061 atacctgcca atttgcgctg atgaaactct tgaccggtgc ggaaggtatg tttaccgccg
     3121 tcggcgacga cgaccagtcc atctacgcgt ggcgcggtgc gaatatggaa aacctgcgca
     3181 aaatgcagga aaactatccg cagatgaagg tcgtcaaact ggagcaaaac taccgctcca
     3241 ccgcgcggat tcttaaaatc gccaacaaag tcatcgaaaa caaccccaag ctgtttacca
     3301 aaaaactttg gtcgcaattg ggcgaaggcg agccggtcaa agtcgttgcc tgccaaaacg
     3361 agcaacacga agccgactgg gtcgtcagcc aaatcatcaa acaaaaactc atcggcggcg
     3421 acaaaaccca atacgccgat ttcgccgtgt tataccgcgg aaagcatcag gcgaggattt
     3481 tcgaggaagc attgcgcggc gtgcgcatcc cctaccggct ctccggcgga caaagctttt
     3541 tcgacaaagc cgaaatcaaa gacgtgttgt cttatgtgcg gctgcttgcc aaccccaacg
     3601 acgatcccgc ctttctgcgc gccgtaacca cgcccaaacg cggcatcggc gatgtaacgc
     3661 tgggcaagct caacgcctac gcgcacgaac acgaatgcag cctgtatgaa gccgcgcaaa
     3721 ccgaagaagc ccttgccacg ctgaacaata ccaaccgcca acacctgcaa gcctttatgg
     3781 atatgttcgg cagctaccgc gccaaagccg aaacaggcga agcaggcgag ttcatcaaca
     3841 gcctgctcga agaaatcgac tatgaaaacc acctgcttgc cagtgaagaa ggcaaagctg
     3901 gcgaaatcaa atggcgcaac gtcggcgatt tggtatcatg gtttgcgcga aaaggcgggg
     3961 aagacggcaa aaacatcatc gaactcgccc aaaccgtcgc cttgatgacg cttttggaag
     4021 gaaaagacga agaagaaacc gatgccgtct cgctatccac gctacacgcc gccaaaggtt
     4081 tggagtatcc ctatgttttc cttgtcggtt gcgaagaagg tgttttgccg cacaacgaca
     4141 gcatcgaaga aggcaacgtc gaagaagaac gccgcctgat gtacgtcggc atcacccgcg
     4201 ccaaacgcca gcttacgctg acccactgcg tcaaacgcaa aaaacaaggc atatggcagt
     4261 tccccgaacc cagccgattc atagacgaaa tgccgcagga agatttgaaa atcctgggtc
     4321 gcaaaggcgg cgagccgatt gtcagcaaag aagaaggcag acgcaacctt gccgatataa
     4381 tcggaaggct cgacaaccta aaaaaaagcg gcgcggcgga ttaaaccgga gccgcaatgc
     4441 cgtctgaagg cttcagacgg catatttttt ggacggcgcg cgtaaagcgg tttacgccca
     4501 caaatcctgc tgctggtttt tcggcacaag atgccccacg ccgataccga tgaggcggaa
     4561 cgcgtcttcc gtctgcggcg ggacgcgcgc catcaacatt tgcgcagcct gcagcagagc
     4621 tgtgcagtcg ggcaatacgg aggaataagt cagcgtgcgc gtgatgatgc ggaaatcgta
     4681 ggtcttcagc ttgagcgtta cactttgggc ttcgacgttt ttacgcgtga tttgccgcca
     4741 caagtcttcg gcaagatggg gcaagtgtcc ggcagcctgc tcgagcggca ggtcttcggg
     4801 cagggtgatt tctgtggaga tttggagacg ttcgcgttcg gctttgacgg ggcgttcgtc
     4861 cgtaccgcgc gccaaatcat agaggcggta gccgtagcgt ccgaaatggt ttaagagctc
     4921 gccgcgctcg aaacggcgca aatcacccgc cgtctgcata cccagcgact gcattttttt
     4981 cagcgttacc ttgcccactc cggggatttt gcccaaaggc agggtttcca aaaatgccat
     5041 gactttgtgc ggcggcaaca caaactgccc gttcggcttg cgccagtccg acgcgatttt
     5101 cgccagaaat ttgttcggcg cgatgcccgc ggatgcggtc aaacccgttt ccgcaaaaat
     5161 ggcggcacgg atttctttgg caacttcgct ggcgtaaggg atgtttttga aattacgggt
     5221 aacgtcaaga taggcttcgt ccagcgacag gggttcgatt aaatcggtat aacgcctgaa
     5281 tacggcatga atctgcgcgg aaacctgacg atacaaatcg aaatgcggcg gcacatacac
     5341 cgcttgcgga cacagccttt tcgccgttgc caccgacatc gcggaatgca gcccgaactg
     5401 ccgtgcctca tacgatgcgg cgcaaatcac cgaacgcgcg ccctcccacg cgacgaccac
     5461 cggccgccct ttcaaatgcg gctgttcgcg cagctctacc gatgcgtaga atgcgtccat
     5521 gtcgatgtgg atgattttgc gtgaagacat cggctctgct gaggataaaa aggatattct
     5581 actgccggca tcgggcaaat tc
//
LOCUS       DAWKQB010000134         5549 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.134, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000134 DAWKQB010000000
VERSION     DAWKQB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.134"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(42..809)
                     /locus_tag="WJL14_001732"
     CDS             complement(42..809)
                     /locus_tag="WJL14_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ7656777.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJL14_001733"
     CDS             complement(897..1724)
                     /gene="mutM"
                     /locus_tag="WJL14_001733"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ7656778.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRSDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1776..2441)
                     /locus_tag="WJL14_001734"
     CDS             complement(1776..2441)
                     /locus_tag="WJL14_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ7656779.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2619..4595
                     /locus_tag="WJL14_001735"
     CDS             2619..4595
                     /locus_tag="WJL14_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ7656780.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDIYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIAAPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPNTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRWVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4663..5355
                     /locus_tag="WJL14_001736"
     CDS             4663..5355
                     /locus_tag="WJL14_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ7656781.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHSLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
BASE COUNT         1456 a         1783 c         1197 g         1113 t
ORIGIN      
        1 ccacaaaagc gaatcatact gccacattcc gacaaccggt ttcagacagc gatatccgca
       61 tcgtcggcga caacggcacg gatgctttct tcgattttat cttttaaagc ataacggtct
      121 tcgctttccg ccgcatccgc cacgcaaacg aaatcgactt ttatcgtcaa ttttttcata
      181 gacacgatgc gccacaggca ggtcggcaaa ccgacatcgg catatgaggg acgagccgtc
      241 ctttttcccg tttcgtcata ataacgcagc gcgaccgcca aaacctttgc ccccgcatcg
      301 atggcggatt ggaacagcgc ggctttgaac ggcaaaagcc ccaatccgga ggaagtccgc
      361 gcttcgggga aaaaactgac gttttgaccg cgttgcaagg tttcgcagac ggcgcggtta
      421 atcggttcga tgtcgcgccg cgaattgcgg ttgatgaaca ccgttcccgc gttctgcccc
      481 atcttgccca ataccggcca gcttttgatt tcctgcttgg cgataaagct gctcggataa
      541 accgcgctca tcgcgaaaat atccagccag gacacgtggt tggcggcaac caagacaccg
      601 ttcggatgtt cgggtgcggg tctgcccacc tccaatccga tatccaaagc cgccaaaacc
      661 cccctgccca actcgattac cgcccgattg cgcgactcgg ggcaaccgcc gtcaataccg
      721 cgcaggtttt tcccggtttt gaacagccag accgtcaagc ggcacaagcg gcgcagacgt
      781 gtaaaaaatg aagctttatt tgaagacatg aacaattctt tcccgataat cggtgtttgt
      841 tgttgaaacg ttcggacggc ataccgataa aatgccgtct gaaaccgttt tccgccctat
      901 ttctgacaat tcgggcagta aaacgtgccg cgctgcccca aagtttcttt cacaaccaaa
      961 ccgccgcact gggggcacgg ctgattgtgc cgcccgtaca ccgtatattc ctgttggaag
     1021 tagccgcttt tgccgtcgct gtccacaaaa tccctcaagg tactgccgcc cgtttcaatg
     1081 gcgcgccgca acaccgcttt gacggtttca accaaaagcg cacactcttt ctttttcaga
     1141 cggttggcag gacggtgggg cgaaatgccc gctctgaaca ggctctcatt ggcataaata
     1201 ttgcccacac cgaccacgac cgcattgtcc atcagggcaa gtttgaccgc gcgcttctgc
     1261 gccttcagcc tcacatacag ataatccgtg caaaatgcct ccgacaaagg ctccggcccc
     1321 agtttttcca acagcggatg atgttcttcg attccctcat accaaagtat cgcgccgaac
     1381 tttctcggat cgcggtaacg catgaccgtg ccgtctgaaa acacaatatc gacgtgatcg
     1441 tgtctgtccg acctgccgat acgtccgtcc gacggcgtaa aaatccgcaa actgcccgac
     1501 atccccaagt gaatcagcaa aatccccgtt tggaaacgga caatcagata tttggcacgc
     1561 ctgccgcagg acaaaacctg ccgaccggac aaaatctccc ccaaatcggg attgatctgc
     1621 cagcgcagct tcaattggcg caataccacg gcttccaccg ttttcccttc aatatgcggc
     1681 gcgatgccgc gcaacgtcgt ttccacttcc ggcaattccg gcataacccc tcccgacatt
     1741 tcttctgaca gatgccgtct gaaagacggc tgtccctaat ccgcaatcct tgccgcaccc
     1801 gccgcaaggg ctttgccgcc caaatacccg tcccacgcgg gcaggggcgt tacgcccgcc
     1861 gcccttttga ccaaaaccaa accgtacact gcggcacact gcggccacca acggtcgccc
     1921 gccttttcca taaaccgcca aaagcgtatt tgcccgagcg acgaaacggg cggcagatac
     1981 accataaatt tcccaaattc aatatcgaaa ccgacatccg caagctgcct tttcaactcg
     2041 ggcagcggca gacaaaaccg tttttccggc aggcgttcgc cgtcaaacca acggctgaat
     2101 ccccagagcg aatacggatt gaaacccgtc agcatcaaac gtccggacgg tttcaatatc
     2161 cggtgcgctt ccgacaggat ttgcgaagga acaccgcctt ccagagtatg cgggaaaagc
     2221 agcatatccg cagaaacatc cgccaaagcc atattttccg ccgacatcga catatctcgc
     2281 ggcacacaga caacatcttc agacaggctc agccacggac cgcccacctg aaccgcacac
     2341 attcccgaaa aacggtatga atccagatac cgcccgaaga aatcctgttc caattttgca
     2401 acataccgcc ccatcgccgt atcttcaaac catgaatcca tattgcgtcc gtttcaaaca
     2461 gattgcctgc cgatattcta ttccaaacag gatttctgtc aaaaaacaca tcggccgccc
     2521 atttccgaat ccgcataaag ttcctgtaaa acttgacgct ttttcagtca aacagtacca
     2581 tcggacgata aaatatgttt attccgccga tataaatcat gtccaaactc aaaaccatcg
     2641 ccctgaccgc gtcaggtctg tccgtttgtc cgggtttcct atacgcccaa aacacctcat
     2701 cacaccaaat cggtttggcg attatgcgct taaactcttc aatactcgac ctgccaccga
     2761 caaaacaata tttccaatcc ggcagcctgt ggagcgagct gcgccaaggc ttccggatgg
     2821 gcgaagtcaa tcccgaactg gtacgccgcc acgaaagcaa attcatcgca agccacagct
     2881 atttcaacag ggtcatcaac cggagtagac cctatatgta ccatatcgcc aacgaagtca
     2941 aaaaacgcaa tatgcccgcc gaagccgccc tgcttccctt catcgaaagc gcgttcgtca
     3001 ccaaagccaa atcacacgtc ggcgcatcgg gcctgtggca gttcatgccc gctaccggca
     3061 ggcattacgg cctggaaaaa acaccggttt acgacggcag gcacgacatt tacgccgcca
     3121 ccgatgccgc actcaactat ctgcaatacc tctatggact gttcggcgac tggccgctcg
     3181 cctttgccgc ctacaactgg ggtgaaggca acgtcggacg cgccatcaac cgcgcccgcg
     3241 cccaagggct cgaaccgacc tacgaaaacc tgcgtatgcc caacgaaacg cgcaactatg
     3301 ttcccaagct gctcgccgtg cgcaacatca ttgccgcccc ccaatctttc ggcatgaata
     3361 tcagcgacat agacaacaaa ccgtattttc aggcagtcga accggaccgt ccgctcgaca
     3421 acgaagccat cgcccggctt gccggcatca cgcaaagcga gctgctcgcc ctaaaccccg
     3481 cattcaacgt ccccgcgttc atccccaaaa gcaaacgcaa actgctgctt cctgtcgcgt
     3541 ccgtacaaac cttccaaagc aactacctca acgccgcacc cgacagcctg ttttcatggg
     3601 aagtctatac gcctgccgcc aaaaccagct tgtccgacat ctcgacggca accggcatga
     3661 gcattgccga catcaaacgc ctcaacaacc tgaacggcaa ccttgtcaac gcaggacgca
     3721 gcatccttgt cgccaagaac ggcaaaaccc ttcagacggc atcggaatcc gtcgtttcca
     3781 tcgacatcga caatacgccc aacacctacc gttccaatat gccggcaggc acggtgaacg
     3841 tcggcattgc ccgaatccga cccgccgccg cacagacagc ggacattacc gtcgcacctt
     3901 tgccgcagaa aaccgtccgt acggaacccg atccccttgt ccgtattgcc gaacctgccc
     3961 ttgcgacagc cgcagcgcaa cctcaaaccg aaaaacagac cgccatgccg tctgaaaccc
     4021 aaaccgcaac actcgcgcag atcgtccccc aaaacgacat gcaggcggca gacgaactca
     4081 tgcagcttgt tgcccgaaac aacctgcgcc gacaggctga agaaaccatc tctgccgtca
     4141 tcggcacgcc tgacacagtt gccgaacaca acatttcctc atctccgcaa cataccgttg
     4201 ccgccgacgg caaacgctgg gtacgtttgg aaacgcgcgt agccaaagct gccgacggcg
     4261 aagccgaaat ctccccgctc catgccagca tccaccgcgt tgtagaaggc gacaccctgt
     4321 tcaacattgc caaacgctac aacgtcagtg tagccgacct gattgtcgcc aacaacatca
     4381 aaggcaacac catccaaaaa ggacaggtac tccgcctggc gcaggcagcc cctgcccaaa
     4441 cccgtattga aaaagtatcc tacaccgcgc gcaaaggcga caccttcaaa agtatcgccg
     4501 cgcgcttcaa tatccatatc gacgacatcc gccggctcaa tcccaacctg aacaccatca
     4561 atccgggaca gagggtcaaa ctgattggaa gctgattcgg atacggcaca tgacaggact
     4621 ttctcagtcc tgtctttttc atcccacatt tcccatacca tcatgaaact tatcaaatac
     4681 ctgcaatatc aaggcatagg aagccgcaag cagtgccaat ggctgattgc cggcggttat
     4741 gttttcatca acggaacctg catggacgac accgatgcag acatcgattc ctcatccgtc
     4801 gaaacgttgg atattgacgg ggaagcagta accgtcgttc ccgaacccta tttctacatc
     4861 atgctcaaca agcctgaaga ttacgaaact tcgcacaaac ccaagcacta ccgcagcgta
     4921 ttcagcctgt tccccgacaa tatgcggaac atcgatatgc aggcggtcgg caggctggat
     4981 gcagatacga ccggcgtatt gctgattacc aacgacggca aactgaacca cagcctgact
     5041 tcgccgagca gaaaaattcc caagctgtac gaagtaacgc tcaaacaccc cacaggagaa
     5101 acgctctgcg aaaccttgaa aaacggcgtg ctgctccacg acgaaaacga aaccgtttgt
     5161 gccgccgatg ccgttttgaa aaacccgacc accctgctgc tgaccattac cgaaggaaaa
     5221 taccaccaag tcaaacgcat gatcgccgcc gccggcaacc gcgtgcaaca ccttcatcgc
     5281 cggcgattcg cacatctgga aacagaaaac ctcaaacccg gggaatggaa atttatcgaa
     5341 tgtccaaaat tctgaaataa catccaagaa ttccatttat attcatccac atactcatta
     5401 aatatatggt ttaacccaat ttaacgcaaa aaatcaattt acgatataat ccatttgtct
     5461 gagtaacgcc tgttcaagca ggcttatgag taagacgttt tccccgtaat gtgttttgcc
     5521 atctatactt ctccccttgt atagatggt
//
LOCUS       DAWKQB010000135         5452 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.135, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000135 DAWKQB010000000
VERSION     DAWKQB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.135"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(254..3091)
                     /locus_tag="WJL14_001737"
     CDS             complement(254..3091)
                     /locus_tag="WJL14_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HEZ7656782.1"
                     /translation="MNRRHTFPLTLTALAIAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGIDRLDAFRQTYDIQKQNKKAEYFLAEGERES
                     KPVAKLAGNGNYLKNQLNRWVEERKKNNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     SNKSGVYDGNDFRDGLYFVPNIEEWKGDTNLVKGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEAYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEHT
                     GEYTADDKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKY
                     FDFSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSF
                     QELFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVA
                     DQKTKLPDSAGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYHKLPEGLYTTLAYN
                     RIKPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDEL
                     AYLAGDQKRYSAKRASSSWSTADVSAYLNLKKRLTLRAGIYNIGNYRYVTWESLRQTA
                     EGTANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3088..5247)
                     /locus_tag="WJL14_001738"
     CDS             complement(3088..5247)
                     /locus_tag="WJL14_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ7656783.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSVETTPVNRPAVGAAMRLLRRNIAFHREDGTAIPDSKQAEEKLSFKEG
                     DVLFLYGSKKDKLQQLKDKIHQRNPNVEIRTSENENKKYGYEFVDAGYVYTTKGKDEI
                     EWTSNHKQFTYRFGYDGFVYYSGEHPSQSLPSAGTVKYSGNWQYMTDAIRHRTGKAGD
                     PSEDLGYIVYYGQNVGATSYAATADDREGKHPAEYTVDFGKKTLTGKLIKNQYVQKKT
                     DEKKPLTIYDITATLDGNRFTGSAKVNTEVKTKHADKEHLFFHTDADQRLEGGFFGDN
                     GEELAGRFISNDNSVFGVFAGKQKTDASNASDTNPAMPSEKHTKILDSLKISVDEATD
                     SNARKFAISPMPDFGHPDKLLVEGREISLLKDTQTIDLADGRKMTVSACCDFLTYVKL
                     GRIKTDRPASKPKAEDENSEDEIGESEENEEDLVAEEENTEDEVVEDEDREEDEVSED
                     GNSEDEEEIAEEDDDEAEEEEVEETEEESPVEESGGDSGSILPTPEAPKGRDIDLFLK
                     GIRTAEADIPKTGTAHYTGTWEARIGVPADGTTFIQKDNYANQGAKAEFDVDFGAKSL
                     SGKLTEKNDTTPAFYIEKGVIDGNGFHALAHTRENGIDLSGQGSTNPKNFKADNLLVT
                     GGFYGPQAAELGGNIIDSDRKFGAVFGAKKDDKEATR"
BASE COUNT         1162 a         1463 c         1275 g         1552 t
ORIGIN      
        1 atttgaaatt gcggcattta tcgggagcaa cagaaaccgc tccgccgtca ttcccacgaa
       61 agtgggaatc cagctttttg agtttcagtc atttccgata aattgcctta gtattgaatg
      121 tctagattcc cgcctgcgcg ggaatgacgg cggaaatttt gtttatatcg aatcaaaaaa
      181 acctgcacct taatcagttg gcggtttagt ccgacttttg gggtgcagat caagctttca
      241 gacggtattt cctttaaaac ttcatttcaa gcgcgaggct gaagttcctg cccggtgcgg
      301 cataccttcc atagttgctg tcgccgccgt gccggtttgc cgtgccttcc gcagtctggc
      361 gcaaggattc ccaagtaacg tagcggtagt tgccgatatt gtagataccc gccctcaatg
      421 tcagccgttt tttcagattc agataggcgg aaacgtctgc cgtcgaccaa gaagacgacg
      481 ctctttttgc cgaatatcgt ttttgatcgc ctgccagata agcaagttca tcagggtttt
      541 tccctttgga ataggtcagc ataatgtttg cgccccattt tccctcgggc tggtcgtatc
      601 cgaaccccaa aacataacgc gacggctgta ccgcatccaa agcatagctg cggagggata
      661 gtcccggccg gttggatacc gatttcggtt tgatgcggtt gtacgccaaa gtcgtgtaca
      721 aaccttcggg cagtttgtga tacacgccgt tccagtcgat ttttcccaag atgttgatgc
      781 cttggagcga catattttgg gcattgtaat aatcgcgtat atcaatctct gtcaatcgtc
      841 ctgccgaatc cggcaatttg gttttttgat cggcaacggc aatcatatcg gtatagcggt
      901 tgcggaagct gctgatttcc aaaaagccga agtcgccttt ccactgcaaa ccgatttcgc
      961 ggttggctgc cttttccgat ttcagggcgg gacgctgcca gcctttcgga taatcgtgat
     1021 aaatgtctat cccgaaaagt tcttggaatg aaggcgttct gaagccgctg gaggcgcggt
     1081 aagacaaaga aaaatgccgg ttcggtttga acacgatgcc gctgttccac gaacgatcaa
     1141 cataccgtcc gctgcggacg agttcttccg acgtggtgaa gtttttccgg tcatacctgc
     1201 cgcccaagct gaaatcgaaa tatttgccga ttgaaaaacg gtcgttcaaa gaaatatgga
     1261 tattgctgcc gttgattttt cttggcacgc atttgcggga acgcagggtt tcgatgtagc
     1321 cgcagaccga cccttcgacg acttcgggct tacccaaacg gtacttgtct ttgtcgtcgg
     1381 cggtgtactc ccccgtgtgt tccgaaatcc ttgccgcatt gtgggaaagc tgttcggggc
     1441 gggagatcgc gttggaagca tcgtaaccga agcccaaagt cagatggtgt ttcgtccatt
     1501 tgtttttcag cgatttctca aacgaggcat tcaaaacatt gtgctgttcg cggtagtgga
     1561 aacggtcgct gctgtcgtag gaatacggtt tgtccgccga cgcgcggcag gatttgtcca
     1621 cggcaggata cacggcgcaa ttcagcttca gcgtgttgtt gtcggttgcc acgccctgtt
     1681 tgtcaaacga caacaccgcc ttatccgccc agttgtcaga atacgcttcg ttttcataac
     1741 gatacagcaa acccatacgg cggcggcggt gatgttcgtc aataaatttg gtgcgggaat
     1801 atttcaaacc tatgcccttg accaaatttg tatcgccctt ccactcttct atattcggca
     1861 caaaatacaa gccgtcgcgg aaatcgttgc cgtcgtacac cccgctcttg ttgctaaact
     1921 ttttcgcctc atccgtaccg taatactgtt tttccgtcat atcgcggatg tcgtaacgct
     1981 gtttggtatc ctcaaacacg ccgccgacat aatgcctgcc gccgaagcgg tagcccagct
     2041 tggcaagcca agagccgctg cggtaatcca tcggatcggg caatatcctg ccgccgcccg
     2101 tgtaagcttg ggcggacagg ttttcgtggc gtgcctgagc ctcccgcacc atcgcttctt
     2161 cttcggcact taaaggctga ttgtttttct tacgttcttc cacccagcgg ttgagctggt
     2221 ttttcaaata gttcccgttg cccgccaatt ttgccacggg cttggattca cgctcgccct
     2281 ccgccagaaa atattccgcc tttttattct gcttctgaat atcgtatgtc tggcggaacg
     2341 cgtccaaacg gtctatgccg tatgccaccc cgtccgcaat atcgccgtgc gggcgcgttt
     2401 cccgcccttg gcgttcggtt cggattagca gcccttccca accgtcttta ctgaatcccg
     2461 cgccgagcga cttcataaat tggcggtttt tactgccgta ggcggttttt gcctgtatcc
     2521 cccagctttt gccgtctgaa atcaggtctg ccgcttcttt ggtgcggaaa gcgaccgcgc
     2581 cgccgagtgc gccgctgccg tgatcggacg aaccggcacc tttgtcaatt tctaccgtgc
     2641 tgatgttttc atattcgatt tcgttgattg caccgctgcc gccgcgtccg ccgtatccgc
     2701 tcaacgatcc ctgcacggta aacgcctgta tttgggcaac gccgtcaacc gaaaccgcca
     2761 cacggttttt atccacgccg cgtatcgagt agccgccgct cgcgccgttg ccctgttcga
     2821 caaccgccac gcccggatcg tagcgcgtca ggtcgcggat accgagtacc tgttctttgt
     2881 tcaacgtttc cgacgttttg acgattttgc ccaaaccggt cgcctctttc gatcgccgtc
     2941 ccactttggc ggcacggacg gtaacctctt tcaaggattg ggtctgcgcc gcatcaggtg
     3001 ccgccgtttc agggtttgcc gcatatgacg ggaaggcggc ggcaatggcc aaggcggtca
     3061 gggtaagcgg gaatgtatgc cttctgttca tcgtgttgcc tccttgtcat ctttcttcgc
     3121 accgaatacc gcaccgaatt tccggtcgct gtcgataata ttaccgccca attccgccgc
     3181 ctgcgggcca taaaagccgc ccgttacaag aagattgtcg gctttgaagt tcttcgggtt
     3241 agtcgaaccc tgcccggaaa ggtcaatacc gttctcccga gtatgcgcca aagcgtggaa
     3301 accgttgcca tcaatcacac ctttttcaat ataaaaagcg ggggttgtat catttttttc
     3361 tgtcaactta cctgaaagcg acttcgcgcc gaaatcaacg tcaaattctg cttttgcccc
     3421 ttgattcgca tagttatcct tttgaatgaa cgtagtgcca tctgccggca cgccgatacg
     3481 cgcttcccaa gtgccggtat aatgcgccgt tccggttttt ggaatgtcgg cttccgccgt
     3541 gcggatacct ttcaggaaaa ggtcgatgtc cctgccttta ggggcttccg gagtgggcag
     3601 gatgctgcct gaatcgccac cgctttcttc caccggcgat tcttcttcgg tttcttcaac
     3661 ttcctcctct tcggcttcat catcatcttc ttcggcgatt tcttcttcgt cttcactgtt
     3721 accatcttcg gaaacttcgt cttcttctct gtcttcatct tctacgactt cgtcttccgt
     3781 gttttcttct tcagcgacca aatcctcttc attttcctcg ctttcaccaa tttcatcttc
     3841 ggaattttca tcttccgcct ttggtttact tgccgggcgg tcggttttta tccgtccgag
     3901 tttcacatag gtcaaaaagt cgcaacaagc actgacggtc attttcctgc cgtcggcaag
     3961 atcgatggtt tgagtatctt tgagcaagga gatttcacgc ccttcgacaa gaagtttgtc
     4021 gggatgacca aaatcgggca taggggaaat ggcaaactta cgggcattgc tatcagtcgc
     4081 ctcgtcaacg gaaattttca gagaatccaa gattttggtg tgtttttcag acggcatagc
     4141 aggatttgta tctgatgcgt ttgatgcgtc tgttttttgt ttgcctgcga atacgccgaa
     4201 tacgctgttg tcgttgctga taaaccgtcc ggcaagctct tctccgttat cgccgaaaaa
     4261 accgccctca agccgctgat cggcatcggt atggaaaaac aaatgctctt tatcagcgtg
     4321 tttcgtcttc acctcggtgt taactttggc actgccggta aagcggttgc cgtccaatgt
     4381 tgcagtaatg tcgtaaatgg tcagcggttt cttttcatcg gttttctttt gcacatactg
     4441 atttttaatt aatttacccg tcaaagtttt cttaccgaaa tctaccgtat attcggcagg
     4501 atgttttccc tctcggtcgt cggcagtcgc agcataagaa gttgctccga cattttgacc
     4561 gtaataaacg atataaccca aatcttcgct aggatctcct gcttttcctg ttcgatgacg
     4621 tatggcatcg gtcatatatt gccagttgcc ggaatatttc accgttcccg cgctcggtaa
     4681 agattgcgaa ggatgttctc cggaataata tacaaaaccg tcataaccaa accggtaggt
     4741 aaactgcttg tgatttgaag tccactcaat ttcatctttt ccctttgtag tatatacata
     4801 acctgcatct acaaattcat aaccatattt tttattttca ttttctgatg tcctaatttc
     4861 tacattagga ttgcgttgat gaattttatc cttaagctgc tgaagtttat cttttttgga
     4921 accgtataaa aacagaacat caccttcttt aaacgacagc ttttcttctg cttgtttgct
     4981 atcgggaatt gccgtgccat cttcacgatg aaaagcaata ttccgcctca acagccgcat
     5041 tgccgcaccg acggcgggcc ggttgaccgg cgtggtttct accgaaggcc cggcaggggg
     5101 cgaagtggga acgtccttag acttgaaagt gacggggtac gcggtcggcg ttgattcgac
     5161 aacaggctgc acgccgaaat tgccgccgat acaagatgcc aaaagtaagg gcaacaagac
     5221 aatgccgcca taattcggtt tacacatccc tacttttcct cctatttgat taataataat
     5281 tatcattata ttaatatgta cagataatat caacccgttt ttatagtgaa ttaacaaaaa
     5341 tcaggacaag gcgacgagcc gcagacagta cagatacatt ccgtcattcc cacgaaccta
     5401 catcccgtca ttcccacgca cctgcatccc gtcattccca cgcacctgca cc
//
LOCUS       DAWKQB010000136         5444 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.136, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000136 DAWKQB010000000
VERSION     DAWKQB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.136"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            195..320
                     /locus_tag="WJL14_001739"
                     /pseudo
     CDS             195..320
                     /locus_tag="WJL14_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(471..1514)
                     /locus_tag="WJL14_001740"
     CDS             complement(471..1514)
                     /locus_tag="WJL14_001740"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ7656784.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1511..1804)
                     /locus_tag="WJL14_001741"
     CDS             complement(1511..1804)
                     /locus_tag="WJL14_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656785.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            2172..2492
                     /locus_tag="WJL14_001742"
     CDS             2172..2492
                     /locus_tag="WJL14_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ7656786.1"
                     /translation="MSIYLDFGISPKTFRQTYLYQKPKLFKGAVRNLEAASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLLYNH
                     IIVD"
     gene            2511..3320
                     /locus_tag="WJL14_001743"
     CDS             2511..3320
                     /locus_tag="WJL14_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7656787.1"
                     /translation="MPRLAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQ
                     LTAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHL
                     AVGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYE
                     AFSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKIS
                     VDELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3549..3902
                     /locus_tag="WJL14_001744"
     CDS             3549..3902
                     /locus_tag="WJL14_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656788.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3963..4679
                     /locus_tag="WJL14_001745"
     CDS             3963..4679
                     /locus_tag="WJL14_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ7656789.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            4744..5238
                     /locus_tag="WJL14_001746"
     CDS             4744..5238
                     /locus_tag="WJL14_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ7656790.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
BASE COUNT         1492 a         1217 c         1375 g         1360 t
ORIGIN      
        1 gggattttag gtttttggtt tgttttcacg gcaatgatgg gattttaaat tgcaggtatt
       61 taccgggaaa aacggcaagc cctccgccgt cattcccgcg caggcgggaa tctagaaatg
      121 aaaaaccacg gtgttatcgg caatgactga aaccggagaa atgacgaaat tgaagcgtgt
      181 ggaaatgtgt cggaaatcgc tgaagccgga taaactatag tggattaaat ttaaatcagg
      241 acaaggcgac gaagccgcag acagtacaga tagtacggca aggcgaggca acgctgtact
      301 ggtttaaatt taatccacta tagattcccg cttttgcggg aatgacggta ttttagattt
      361 ttgttttcgc ggaataatga gccaaaagcc gtccgaaccc aaaaaaaccg tcgctttcag
      421 acggcatcaa aaaaccgcag gcacgatgcc tgcggtttgc cgtttgcgct tcaggggagc
      481 agggggattg cgcccaagcc gtgtgtttcc ttcagtccga acatgatgtt catattttgc
      541 accgcctgac ccgccgcgcc tttgacgagg ttgtcgatga cggaaaggac gacccacaca
      601 tcggattgcg ccgcctgttg gatgctgatg cggcagaggt ttgcgccgcg cacgctgcgg
      661 gtttcgggtg tcgaaccggc gggcaggatg tccacgaaca ggctgtcgcg gtaatagtcg
      721 cgcaggatgg tttcggggca gatgccgtct gaaaggtgga ggtaaacggt ggcgtgcata
      781 ccgcgtatca tcggcgcgag gtgcggcgtg aacacgaatc cttcggcgat gccgtcctga
      841 agcccggcga tggtctgcct gatttcgggc aggtggcggt gtccagctat gccgtatgct
      901 ttgaagttgt cgccgacttc gcacaacagc gaaccgacat tgcctttcct gcccgcgccg
      961 gacacgccgg atttgcagtc ggcaatcagc ggcataccgg gcttcagacg gcattgccgc
     1021 aacagcggca cgagcggcag ggatacgcag gtcgggtagc agccggggtt ggcgacgagg
     1081 cgtgcctgtg cgacggcttc gcggttgagt tcgctcaatc cgtacacggc ttgggaaacg
     1141 aggtcggggg cggcgtgggt catgccgtac cagtgttccc aggtcggaat gtcccgtatg
     1201 cggaagtcgg cggaaaggtc gatgacgcgc acgccctgtt caatcaggcg cggcgcgtct
     1261 ttcatggcga tgccgttggg cgtggcgaag aagacgatgt cgcattgttc caaacctgcc
     1321 tcgtcgggcg tttggaaggc gaggccgtac acgccgcgca aactcggaaa gtaatcggca
     1381 actgcagttc ccgcttcgct gcggctggtt acggcggcga cttcgacatc gggatgggcg
     1441 gcaagcaggc gcagcagttc cacgcccgtg tagcccgtcg cgccgacaat gccgactttg
     1501 atttttttac tcatggtttt tcctttgtgt ggttggcggg tatgccgtcc gaacgctgtc
     1561 cgacggcttg tccgacgggg ttttgattgg cggcggaagg acggtacaca aagcgtttga
     1621 aaagcgtgtc taatttaatg gcatccgcct ctgtttttaa aacccagccc tgccgtccgg
     1681 aataaacata accgtaggcg cgaaagtcgg caggatattt cggtgcctgc cccaaaccca
     1741 aaccgtatgc ggcgaaagcg gtggtcgtgg agaaggaaag caggaggagg acgagggttt
     1801 tcataaggtt ccaaaccgaa cggggcgatg aagcccgatt atagcaaaag gtcgtctgaa
     1861 aaccgttttc agacgacctt ttatttaatt acaataaaaa tgttaacaac aaaaaacaaa
     1921 ccgctttttt tcgtttggaa acaaaacaaa tttttatagt ggattaaatt taaaccagta
     1981 cagcgttgcc tcgccttgcc ttgccgtact atctgtactg tctgcggttt cgtcgccttt
     2041 tcctgatttt tgttaatcca ctataactaa aattgtcaac aattccttga cgcacacaca
     2101 aaaaaaactt taatatttgt aaatatatgt aacattataa attttaaata ttttctgagt
     2161 aaggggaagt aatgagcatc tatctcgact tcggcattag tcctaaaacg ttccgacaga
     2221 cttatctgta tcaaaagccc aagctcttta aaggagcggt tcggaatctc gaagccgcat
     2281 cttggaaaga tatcaacgag atataccaac gagcagaccc aaccgcaccg ctgtttcatc
     2341 tgcgtaaaaa aggtgcaatt gttcctaaag aagaatacgt cgaaagtttc gacgatttgg
     2401 gcaaaactcg ctaccgtttt attaaatccg ttatctacga acatatgaag aatggtgcgt
     2461 cgttgctcta taaccatatt atagtggatt aaatttaaac cagtacagcg ttgcctcgcc
     2521 ttgccggcgc acatactatt gttagtgggt atcttgcttt tggcagcgac gaatcttata
     2581 aaaaccattg ggacatccga gatgtgtatg ccatccagct tttcggcaag aaacgttggc
     2641 aacttactgc ccctgatttc cctatgccat tgtatatgca acagactaaa gatactgata
     2701 tttccattcc tgaacatatc gatatggata ttatccttga agcaggtgat gtcctctaca
     2761 tcccgcgcgg ttggtggcac agacctatcc cgctcggctg tgaaaccttc cacctcgctg
     2821 tcggtacctt tccgcccaac ggctataatt acctcgagtg gctaatgaag aaattcccca
     2881 ccatagaaag tctgcgccac agtttctcag actgggagca agataggacg cgtatcaacg
     2941 atactgccgc acaaattgct gccatgattg ccgaccccgt caattacgaa gccttcagtg
     3001 aagacttcct cggcaaagaa cgtaccgata ccgcttttca tctcgaacag ttcgcgaatc
     3061 ccaacgctac tccgctttca gacgacgtca ggttgagatt aaatgccaat aatttggata
     3121 cgttggaaaa gggatatttg attgggaatg ggatgaagat aagcgtagat gagttgggga
     3181 aaaaagtgtt agaacacatc ggtaagaatg aaccgttatt gttgaaaaat ctactggtta
     3241 acttcaatca gggaaaatat gaagaagtta ggaagttgat ttatcagttg atagagttag
     3301 attttctgga aattttgtga gggattctat gaaaaactgg aagcaattta catttttcgt
     3361 aatattagta atagcttgtt atcaagtgct atatttttta tcagatatgt ttcttctcga
     3421 ctacattaac aaatatagtt ggaatttgaa ttttattcag ggtactttga attttttttc
     3481 aatatatctg ccatatgttt ttgttagccg aatttttagg aataccaacc aagaaaagga
     3541 gtataaaaat gattgaactt caacttcatg aattgaagct ggtttcaggg ggaggtcctg
     3601 taacagacaa tatagctgga aatgtagcta atgctgccac aaccaaagga ggtcccacat
     3661 ggggggattt ggttgcaata cctgctgccg cagcggcagt ttattatctg ccgaaaaatg
     3721 cttacggtgc tgctggtgca aatggtgtat acaatgtgac tcgtaattgg gtaaatgatg
     3781 ccgttaatgc acctccttat aacggaagac caatctttga gattgaacat ggattaactg
     3841 ctcccgcaac aaaagcagat aaatcaggaa acggctacac tgacggtaca gattactgct
     3901 gatatttctc atcgtccaga caatctctag gggtcgtctg aaactttctt aacttcaatt
     3961 ttatgaatag acccaagcaa cccttcttcc gtcccgaagt cgccgttgcc cgccaaacca
     4021 gcctgacggg taaagtgatt ctgacacgac cgttgtcatt ttccctatgg acgacatttg
     4081 catcgatatc tgcgttattg attatcctgt ttttgatatt tggtaactat acgcgaaaga
     4141 caacagtgga gggacaaatt ttacctgcat cgggcgtaat cagggtgtat gcaccggata
     4201 cggggacaat tacagcgaaa ttcgtggaag atggagaaaa ggttaaggct ggcgacaagc
     4261 tatttgcgct ttcgacctca cgtttcggcg caggaggtag cgtgcagcag cagttgaaaa
     4321 cggaggcagt tttgaagaaa acgttggcag aacaggaact gggtcgtctg aagctgatac
     4381 acgagaatga aacgcgcagc cttaaagcaa ctgtcgaacg tttggaaaac caggaactcc
     4441 atatttcgca acagatagac ggtcagaaaa ggcgcattag acttgcggaa gaaatgttgc
     4501 agaaatatcg tttcctatcc gccaatgatg cagtgccaaa acaagaaatg atgaatgtca
     4561 aggcagagct tttagagcag aaagccaaac ttgatgccta ccgccgagaa gaagtcgggc
     4621 tgcttcagga aatccgcacg cagaatctga cattggccag cctcccccaa gcggcatgag
     4681 acagaacaaa gctggcttga acgcaccatt gccgatattt ctcaagaagt tttggatttt
     4741 gaaatgcgct ctgaacaaat catccgtgca ggacggtcgg gttatatagc aataccgaac
     4801 gtcgaagtcg gacagcaggt tgatccttcc aaactgctct tgagcattgt tcccgaacgt
     4861 accgagctat atgcccatct atatatcccc agcagtgcag caggctttat caagccgaaa
     4921 gacaaggttg tcctacgtta tcaggcatat ccctatcaaa aattcgggct tgcttccggc
     4981 agtgtcgtat cagtagcaaa aacggcactg ggcagacagg aattgtcggg attgggcatg
     5041 gtatcctccg atttggcgaa gagcaacgaa cctgtttatc tcgtgaaaat aaaacccgac
     5101 aaaccaacca tcactgcata cggtgaggaa aaaccgctgc aaatcggcat gacgttggaa
     5161 gcagacatcc tgcacgagaa acggcggctg tacgaatggg tattggagct gatttatagt
     5221 atgtcgggca aactgtaaga cttgaggctg aaagataaag aagttgtctg aaaccttttc
     5281 agacgaccag aagacaataa aaaacacaaa cttcaaaact gcctgaaacc atcctctttt
     5341 acaggcactc cgtctgtaaa agcaaggata gggcatctgc ccttttgatt gtttgattaa
     5401 cgatacaagg agtttcaaaa tgagagtttt atagtggatt aaca
//
LOCUS       DAWKQB010000137         5413 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.137, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000137 DAWKQB010000000
VERSION     DAWKQB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.137"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1805
                     /locus_tag="WJL14_001747"
     CDS             <1..1805
                     /locus_tag="WJL14_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7656791.1"
                     /translation="IEKLAKQPEYAYLKQLQTAKNVDWKQVQLAYDKWDYKQEGLTGA
                     GAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTVSTAAAMQTAALAS
                     LYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQMGADIAQLNSKVRT
                     ELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLGNAALGALVNSFQG
                     EAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVGEIVAESILGGRNP
                     ATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNALNFDSTPTNAKKH
                     QPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIRNGITGPIVITSYGVYA
                     AGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAVTVGNAWEAPVGAL
                     SKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQPVSAGF
                     EHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTVDVGKVI
                     GTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            1812..2321
                     /locus_tag="WJL14_001748"
     CDS             1812..2321
                     /locus_tag="WJL14_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656792.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            2381..4069
                     /locus_tag="WJL14_001749"
     CDS             2381..4069
                     /locus_tag="WJL14_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7656793.1"
                     /translation="MRSMIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLF
                     EAPRLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVN
                     WNQVQLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFA
                     SLASQASVSLINNKGDVGKTLKELGRSRTVKNLVVAAATAGVSNKLGASSLATWSETP
                     WVNNLNVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKGLDQHY
                     VAHKIAHAVAGCAAAAANKGKCQDGTIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVK
                     KITAYAKLAAGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNG
                     KINVRDLKQTLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKGEAAQK
                     VKEVLEKNRPYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQ
                     GLLKTGDRFYLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            4078..4455
                     /locus_tag="WJL14_001750"
     CDS             4078..4455
                     /locus_tag="WJL14_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656794.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            4733..5107
                     /locus_tag="WJL14_001751"
     CDS             4733..5107
                     /locus_tag="WJL14_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656795.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
BASE COUNT         1723 a         1084 c         1245 g         1361 t
ORIGIN      
        1 aaatcgaaaa gctggccaaa cagcccgaat acgcctacct gaaacagctt cagacggcca
       61 agaacgtcga ttggaaacag gtgcagctgg cctacgacaa atgggactat aaacaggaag
      121 gcttgaccgg agccggtgca gcgattgtgg tgattattgt aaccgctcta acttatggat
      181 acggagcggc tgcagcgggt agcgtaactg ccgcaggaag tagtacagcc gcagctgcaa
      241 caacgacagc ggcagcaact accactgttt ctactgcagc tgccatgcaa accgcagctt
      301 tagcctcctt gtatagccaa gcagctgtag ccatcatcaa taataaaggc gatgtaggca
      361 aagcattaaa agatctcggc accagtgata cggtcaagca gattgttact tccgcactga
      421 cggcgggtgc attaaaccag atgggagcag atattgccca attgaacagc aaggtaagaa
      481 ccgaactgtt cagcagtacg ggcaatcaaa ccattgccaa ccttggaggc agattggcca
      541 ccaacctcag caatgcaggt atctcagctg gtatcaatac cgccgttaac ggcggcagct
      601 tgaaagacaa cttaggcaat gccgcattgg gagcattggt taatagcttc caaggggaag
      661 ccgccagcaa aatcaaaaca accttcagcg acgattatgt tgccaaacag ttcgcccacg
      721 ctttggcggg ttgtgttagc ggactggtac agggaaaatg taaagatggg gcaattggcg
      781 cagcagttgg ggaaatcgta gctgaatcca tacttggcgg cagaaaccct gctacactca
      841 gcgatgcaga aaagcataaa gttatcagtt actcgaagat tattgcgggc agcgtggcgg
      901 cactcaacgg cggcgatgtg aatactgcgg cgaatgcggc tgaggtagct gtggtgaata
      961 atgctttgaa ttttgacagc acccctacca atgcgaaaaa gcatcaaccg cagaagcccg
     1021 acaaaaccgc actggaaaaa atcatccaaa gtattatgcc tgcacatgcg gcaggtgcga
     1081 tgactaatcc gcaggataag gatgctgcca tttggataag caatatccgt aatggcatca
     1141 caggcccgat tgtgattacc agctatggag tttatgcagc aggttggaca gctccgctga
     1201 tcggtacagc tggtaaagca gctatcagca cctgtatggc taatccttct ggttgtactg
     1261 tcatggttac gcaggctgct gaagcgggcg cgggaatcgc cacgggtgcg gtaacggtag
     1321 gcaacgcttg ggaagctcca gtaggagcgt tgtcgaaagc gaaggcggct aagcaggcta
     1381 taccaaccca gacagttaaa gaacttgatg gcttactaca agaatcaaaa aatataggtg
     1441 ctgtaaatac acgaattaat atagcgaata gtactactcg atatacacca atgagacaaa
     1501 cgggacaacc ggtatctgct ggctttgagc atgttcttga ggggcacttc cataggccta
     1561 ttgcgaataa ccgttcagtt tttaccatct ccccaaatga attgaaggtt atacttcaaa
     1621 gtaataaagt agtttcttct cccgtatcga tgactcctga tggccaatat atgcggactg
     1681 tcgatgtagg aaaagttatt ggtactactt ctattaaaga aggtggacaa cccacaacta
     1741 caattaaagt atttacagat aagtcaggaa atttgattac tacataccca gtaaaaggaa
     1801 actaactaaa tatgagtaac tttgaaaaaa aatatatttt agaattaaat gatgctttaa
     1861 gccatttaaa tcataactct acctcatttg atttattgaa agttttgatt tcatggttat
     1921 caaacgatat tgtcattgat aaatttaaaa ttttaggtta tgactttagt aaatatatcg
     1981 aaatgaatcc cgatgactat ccggttgaaa aatctatatt gaatagagag gaaattattt
     2041 atctcaaaaa caatatttat cgtaaaatat catcaggaaa ttttaaattt caatactttg
     2101 tacaatatat tagagatatt ttagaatatt tatttattga acatattgaa agagtctgtc
     2161 cttactgcga atggggtgaa atgcaaaaat tagaagaaca aaatacgcat gaaacggtgt
     2221 atctctgtac tcaatgtgga tgtgcttttt ataacgataa ttcacaattt ttattaaaaa
     2281 cccctttaac cattccaatg aaacgtgatg aatttaaata aacaagccgt agcctgcatg
     2341 aaccctaaaa tccacgtgta gcgtgtgtgc gccagcacgc atgcgttcca tgatttacgg
     2401 ctcaatgccg tctgaaaagc tcacaatttt tcagacggca tttgttatgc aagtaaatat
     2461 tcagattccc tgtatgctgt acagacgcgg gagtgttaag ccccccttgt ttgaagctcc
     2521 gcggctcctg ccgagcttca ccgaccccgt tgtgcccaag ctctctgctc ccggcggcta
     2581 cattgtcgac atccccaaag gcaatctgaa aaccgaaatc gaaaagctgg ccaaacagcc
     2641 cgagtatgcc tatctgaaac agctccaagt agcgaaaaac gtcaactgga accaggtgca
     2701 actggcttac gataaatggg actataagca ggaaggctta accagagccg gtgcagcgat
     2761 tatcgcgctg gctgttaccg tggttactgc gggcgcggga gtcggagccg cactaggctt
     2821 aaacggcgca gccgcagcag cggccgatgc cgcctttgcc tcactcgctt ctcaggcttc
     2881 cgtatcgctc atcaacaata aaggcgatgt cggcaaaacc ctgaaggaac tgggcagaag
     2941 ccgcacggta aaaaatctgg ttgtagcggc ggcaacggca ggcgtatcca acaaactcgg
     3001 tgcctcttcc cttgccactt ggagcgaaac cccttgggta aacaacctca acgttaacct
     3061 ggccaatgcg ggcagtgccg cgctgatcaa caccgctgtt aacggcggca gcctgaaaga
     3121 caatctggag gcaaatatcc ttgccgcttt ggtcaatacc gcgcatgggg aggcggcgag
     3181 taagattaaa ggactggatc agcactatgt cgcccacaaa atcgctcatg ccgtagcggg
     3241 ctgtgcggcg gcagcggcga ataagggcaa atgtcaggac ggcacgatcg gtgcggctgt
     3301 gggtgagatt gtcggggagg ctttggttaa aaataccgat tttagcgata tgaccccgga
     3361 acaattagat ctggaagtta agaaaattac cgcctatgcc aaacttgcgg caggtacagt
     3421 tgcaggcgta acgggaggag atgtcaatac tgctgcacaa accgcacaaa acgcggtaga
     3481 aaataatgcg gttaaagctg ttgtaactgc tgcaaaagtg gtttataagg tagccagaaa
     3541 aggattaaaa aacgggaaaa tcaacgttag agatttaaaa cagacgttga aagacgaagg
     3601 ttataattta gccgacaacc tgaccacctt attcgacgaa acattggatt ggaacgatgc
     3661 caaagccgtt attgatattg tcgtcggaac agagctgaat cgcgctaata aaggggaagc
     3721 ggcacaaaag gtcaaggaag ttttagaaaa aaatcgtcct tatatcccta ataaaggtgc
     3781 tgtaccgaat atgagtacat acatgaaaaa taatcctttt ggaaaacagc tggctcaaat
     3841 ttcagaaaag acaacgcttc cgacgcagca agggcagtct gtcttcttgg taaaaagaaa
     3901 ccaagggtta ttaaaaaccg gtgataggtt ttatttagat ggccaacata aaaatcattt
     3961 agaggttttt gataaaaatg ggaactttaa gtttgttcta aatatggatg gttcgcttaa
     4021 ccaaatgaaa actggggcag caaaaggtcg taaattaaac ttaaaatagg aaattttatg
     4081 gaaacattga atgatataaa aaaaatcttg attaatgtgg ggctttatca agggtttgat
     4141 ttgacagatc caaaagtatc agaagaagtt aatcatgaaa cagctaatat gaaatggatt
     4201 aaagattata cttcagacgg gaattgggat aatgaattta aggaggattt aaaaaacttt
     4261 ttagattata tggaagtatg ccaattagcc ctaaacgata aaaatttcaa aattgccagt
     4321 aattctttat ttatggctat gatttacgca ggtaatctat ctcttatatt tgattcaata
     4381 aaaactgata tatcaacatt attgagtgct gagtataaaa agaatagttt ttcatggcca
     4441 tctcttgatg aatagaaagc aagttgtagc ctgcatgaaa tctaaaaccc atgcataagg
     4501 tgtgggcttc agtatacgcg ttccatgatt tacggccata tgccgtctga aaagctcaat
     4561 tttttcagac ggcatttgtt atgaaagtaa atatttagat tccctgtata ctgtttagac
     4621 tcgtgtgtgc tgagtaagct gtagtctgca tgaaacctaa aactcgctca aaattaagct
     4681 aagacattag cagggcaagg gcgaaaattg aatcttaaat aaggtgattc agatgaaaaa
     4741 ttttaatgta gtaaaagaaa gtttaagaga gttaggaatt aaacaaggat ttgatcttta
     4801 tgagaaagcc acaactgaaa aattgaatag tgaagatcct cttgacttac aatggctttc
     4861 taactattca tctgattgga atgatgaatt agaagaagac tttgattctt tttttcagca
     4921 tatgaaggaa tatcaatatg ctattgacaa tgaagacatt aaatctgcat gtagttcact
     4981 atgtgaagct atgctctatg ttggtaatat taaaaatttt tttgagtttc tcaaaagcga
     5041 tatgattaga ctgttgagag gtgaaagtaa aacaacagac tttcaatggc cgcaatttga
     5101 tgaatagcag caagctgtag cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctt
     5161 cagcgcgcac gcgttccatg atttacggct caatgccgtc tgaaaagctc aattttttca
     5221 gacggcattt gttatgcaag taaatattca gattccctat atactgccca gacgcgtgcg
     5281 tgctgaagac acctcctacg cttgctgcag aactttcggg taaaaccggt gtgagcatta
     5341 gcgcaccgta tgccaatgag aacagtcgca tcctgctcag caccacggat atcagttcgg
     5401 aaaacggcaa aat
//
LOCUS       DAWKQB010000138         5364 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.138, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000138 DAWKQB010000000
VERSION     DAWKQB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.138"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1210..1455)
                     /locus_tag="WJL14_001752"
     CDS             complement(1210..1455)
                     /locus_tag="WJL14_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656796.1"
                     /translation="MLKSIELNSHIRNRLAEYLKGRGMDFQTAMQEEKGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            1567..1995
                     /locus_tag="WJL14_001753"
     CDS             1567..1995
                     /locus_tag="WJL14_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ7656797.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDVPRGNPEYV
                     RLSGMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLMLLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRMFNEIPVLLMVAALYLVVFKPF"
     gene            2007..2483
                     /locus_tag="WJL14_001754"
     CDS             2007..2483
                     /locus_tag="WJL14_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ7656798.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISELSRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2833..3321)
                     /locus_tag="WJL14_001755"
     CDS             complement(2833..3321)
                     /locus_tag="WJL14_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ7656799.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(3389..4444)
                     /gene="aroG"
                     /locus_tag="WJL14_001756"
     CDS             complement(3389..4444)
                     /gene="aroG"
                     /locus_tag="WJL14_001756"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ7656800.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGGNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWDATEE
                     LLALLAGANKKRMARAG"
     gene            4796..5001
                     /locus_tag="WJL14_001757"
                     /pseudo
     CDS             4796..5001
                     /locus_tag="WJL14_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5090..>5364
                     /locus_tag="WJL14_001758"
     CDS             5090..>5364
                     /locus_tag="WJL14_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656801.1"
                     /translation="MRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKS"
BASE COUNT         1340 a         1328 c         1273 g         1423 t
ORIGIN      
        1 cagtgtcatg agaaattttc ctttgtcaag tgttaaaatt ataatgatta tatactacat
       61 tatactacat cgcaatgaaa aatcaggaaa agcaaaaaac cctccgccgt cattcccgcg
      121 aaagcgggaa tctagacctt tcggtgcgga aacttatcgg ataaaacggt tttccctgca
      181 tcctggattc ccactttcgt gcgaatgacg ggatataggt ttcactacgg acgtgttcag
      241 attaccgcct gcgcgggaag gacgggattt caggttgctg tttttgattt tctgtttttg
      301 tgggaataac gggactttgg atgatgtgca tttatcggga aaacaaaaaa ccctccgccg
      361 tcattcccgc gaaagcggga atctagaatt ttaatgcggc aagaatttat cggaaaaaac
      421 tgaagtttaa agacctagat tcccgcctgc gcgggaatga cggtttagaa atcacctgaa
      481 acgagaaaaa gaaccgaaac cggacaagtc ggattcccgc acgggcggga aggacggaat
      541 ttcagattgt tgtttttgat tttctgtttt gtgggaataa cgggattttg gatgatgtgc
      601 atttatcggg aaaactaaaa accctccgcc gtcattcccg tgaaagcggg aatctagaat
      661 ttcaatgccg caagaattta tcggggatgt ccggggttta aaggtctgga ttcccgcctg
      721 cacgggaatg acggtttaga aatcacctga aacgagaaaa agaaccgaaa ccagataagt
      781 cggattcccg cacgggaggg aaggacggga tttcaggtta ctgtttttga ttttctgttt
      841 tgtgggaata acgggatttt ggatgatgtg cattttcggg aaaactaaaa accctccgcc
      901 gtcattcccg cgaaagcggg aatctagaat tttaatgcgg caaaaattta tcggaaaaaa
      961 ccgaagttta aagacctaga ttcccgcctg cgcgggaatg acggtttaga aatcacccga
     1021 aacgagaaaa agaaccgaaa ccgaacaagc cggattaccg cctgcgcggg aatgacggct
     1081 caaaagttac gaaacgaaaa ataaccgaaa ccaaacaagt cggattcccg cgcgggcggg
     1141 aatgacgata agcgtaattt caatctgcca tattgtcttt tcacttgcct gaaaacagat
     1201 ttcagccacc tatccctgca tccggcggct gatgtagtcg gcaatcaaat cccgcttttc
     1261 ccaaaaaaac ttctgcattt tttgttcgga atacagtttg cggaccagag tgggcaaacc
     1321 gctgtggacg atggcggcga tttctttgtt gcctttttct tcctgcattg ccgtctgaaa
     1381 atccataccc ctgcctttca gatattctgc aaggcggttg cggatgtggg aattgagttc
     1441 gatggatttg agcattttgc ctccgttttg cggtaaggat ttattcgatt tgtttatccg
     1501 cctgatttta tcacgtttcc caagcggttt gcctgtacaa taccgctttt cggcagggtg
     1561 tgggcgatga tgttttcttg gttcaagctg tttcacttgt tttttgtcat ttcgtggttt
     1621 gcagggctgt tttacctgcc gaggattttc gtcaatatgg cgatgattga tgtgccgcgc
     1681 ggcaatcccg agtatgtgcg tctgtcgggc atggcggtgc ggctgtaccg ttttatgtcg
     1741 ccgttgggct tcggcgcggt cgtgttcggc gcggcgatac cgtttgccgc cggctggtgg
     1801 ggcagcggct gggtacacgt caaactgtgt ttgggcttga tgctcttggc ttaccagttg
     1861 tattgcggcg tgctgctgcg ccgttttcag gattacagca atgctttttc acaccgctgg
     1921 taccgcatgt tcaacgaaat ccccgtgctg ctgatggttg ccgcgctgta tctggtcgtg
     1981 ttcaaaccgt tttgacggag catcttatgc cgatagaaat cgaacgccgt tttttgattg
     2041 gaaacgacaa ctggcggcaa tacgccgacg aaccgctgct gttgaggcag ggttatctgt
     2101 ctgttgaaaa agaacgcacc gtccgtgtcc gtattgccgg acaacgggcg tggctgacgc
     2161 tgaaaggcta tatttcggaa ctcagccgca gtgagtttga atacgaaatc ccgcttgccg
     2221 atgcggaaaa gatgatggaa acgatgtgtc cgtttaagat ggaaaaatgg cgttatccgg
     2281 tcagatgggg cggcagcctg tttgaaatcg atgtttttct tggcgataat gcgcctttgg
     2341 tcgtcgcgga aatcgagttg cccgacgaga atgccgattt tgaccgtccg gactggctgg
     2401 gacgggaaat cactgccgac ggtatgttta ccaatgccta tttgagcagg catccgttct
     2461 caagctggaa aaatgccgtc tgaagcggct tcagacggca ttctccgaca accatcgcaa
     2521 atccaaagac agcctctata tccttatttc tgctttcttt gttccattat tcccaataaa
     2581 tccccataac ctaaaatctc gtcattcccg cgcaggcggg aatccggttc tttaaacttc
     2641 ggttttttcc gataaattgc tttagctttt cgtttccgga ttcccgcttt tgcgggaatg
     2701 acgatggaag attgttgtta tttcggataa attaccctaa ctttaaaatc ccggattccc
     2761 atcttcgggg gaataacaat attttctatt tcagcccatc atttgcgggg gattttatag
     2821 agagtttata tttcaatatc tcaaataatg cacaaaagca tagctcgtgc ctttgctgct
     2881 gacacggcgt tccgtccgct ctgcttcttt ccaatgcgtc cggtctattg cggggaaaaa
     2941 tgcatctcct tccacagaca aatccacttc ggttatccgc aaatcggtcg ccaatggcat
     3001 cgcttgtccg tatatctgcg cgccgcccat aatgacggct tcttccgcgc ctgcgcacaa
     3061 tgccaatgcc gcctccaaac ttgccgccgt ttccgcgcct gccgcgcaat aatccgcctg
     3121 ccggctgatg acgatgttcc tccgtccagg caggggtttg acgggcaggg attcccacgt
     3181 tttccgcccc ataatgacgg gtttgcccaa ggtataggct ttgaaaaatg cgaaatcttc
     3241 ggggatgtgc caaggcatag cattgcccgc cccgatgcac aggttttccg cacacgccgc
     3301 aattagggtt attttgagca tatttcctcc acaaaaatgc cgtctgaacg cttcagacgg
     3361 cattttatga cattctgcgt caaaaatctc aaccggcgcg cgccatacgt tttttgtttg
     3421 cacctgccaa caatgccaac agttcttcag tcgcgtccca accaatacac gcatcggtaa
     3481 tgcttttgcc gtacacttcc ggcttgtcct gcctgccttc gaccaaatgg ctttccacca
     3541 tcacgcccat aatattgccg ccgtcctgtt ccaattgggc ggcaatgtct tgcgccactt
     3601 ccatctgtcg ggtgtaatcc ttgcggctgt tggcgtggct gcaatcaatc atcagcttgt
     3661 cggttacgcc tgccgcacgc agttgttccg ccgcctcgct gacgtgtccc gcatcataat
     3721 tcggctcttt tccgccgcgc aaaatgacat gacagtcagg attgccgccg gtatggacaa
     3781 tggcggaatg cccggccttg gttacagaca ggaaatgatg cgaatggctc gccgcaccga
     3841 ttgcgtcgat ggcaatcttc aaattgccat ccgtaccgtt tttaaagccg acggggcagg
     3901 acagcccgct tgccaattcg cggtgaactt ggctttcggt cgtccgcgcg ccgattgccc
     3961 cccaagaaat caagtccgca taatattgcg gcgtaatcat atccaaaaac tcggtagagg
     4021 caggcatacc catattgttc agcgacaaca acaggctgcg tgcctgacgc aaaccgaaat
     4081 tgatgtcaaa cgtaccgtcc aaatgcgggt cgttaatcaa acctttccaa cccaccgtcg
     4141 tcctcggctt ctcgaaataa acgcgcatca caatcaaaag ctcgttttca tactgcttgc
     4201 ggagtttcaa caaacgctcc gcatattcca acgccgcttt cggatcgtga atcgaacacg
     4261 gcccgataat aaccaacagc cgcttgtccc tgccgtgaac caaatcggaa atttcctgac
     4321 gggtgcggtg aaccaagccc gaagcctctt tggaaatcgg cagctcgtaa agatgggcaa
     4381 tcggcggcaa caactcttta acttctttaa tcttaatatc gtctgtgggg taatggtgtg
     4441 tcatcttgca tctttcctga tgggttttta tacgcggctt aggttacatc gtttaccttg
     4501 cacaaacaag tgcaacatat tactacaaag ctattttatt tcaaaataac tgatatatct
     4561 tttttcatag attttaaatt tcatattttt aatattttag gaattttatt ccaaagaaaa
     4621 agaaacagat tgataaaaac aatacccgcc attcattcgg gcaaaaccgc acggaacatc
     4681 cgttttccca tttccacgac aatccggtac agaacacccc gctgccgatt ccgcctgcac
     4741 atccggcaaa atttcatacg caaggaaaat gttgaaaaca aaaagaactc attgcttgaa
     4801 aaaagattta atgggaatgc gggtatttcc cacacttttt ccatttattt atcctcatta
     4861 taggaagaaa aacatgaaaa aaccctgctt gcactctcac ttgcagccgt gtccgccgtt
     4921 acggtagccg atattcccga gtctaacggc aaaggcaaag ccatcatcgg cgataaggaa
     4981 aaacaccgtt accaagcatg aaaaccttgc tttgaaagtc catggcgaaa tccgccaaga
     5041 tttgggggac ggatgatccc taaccccttg ggcggatgca tatgcagatt tgcgcggcaa
     5101 aaccaaagtg atgacgaccc agatgtgtgc ttcccgcgat gtcagcaaaa gcgccaaagg
     5161 ttggagtgtc ggtatcggtc tgaatgtagg caaacagttg accgacagcg tcggtctcga
     5221 gtttgatcca tactaccgtc acaaaacaat ctgcaaaccc cgtgagattg ttttggacgg
     5281 cgacaaaacc aaaatgggcc gctccaaatc caacgagtac ggcttccgcg taaccgcaac
     5341 gttctatagt caattaaaat caaa
//
LOCUS       DAWKQB010000139         5300 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.139, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000139 DAWKQB010000000
VERSION     DAWKQB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.139"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(220..295)
                     /locus_tag="WJL14_001759"
     tRNA            complement(220..295)
                     /locus_tag="WJL14_001759"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Lys,seq:ttt)
     gene            complement(364..1338)
                     /locus_tag="WJL14_001760"
     CDS             complement(364..1338)
                     /locus_tag="WJL14_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ7656802.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            complement(1371..1820)
                     /locus_tag="WJL14_001761"
     CDS             complement(1371..1820)
                     /locus_tag="WJL14_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ7656803.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            complement(1861..3132)
                     /gene="waaA"
                     /locus_tag="WJL14_001762"
     CDS             complement(1861..3132)
                     /gene="waaA"
                     /locus_tag="WJL14_001762"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ7656804.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRRTGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRQVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TVKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSSEEGGMQMQ
                     ARADGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            complement(3195..4643)
                     /gene="gnd"
                     /locus_tag="WJL14_001763"
     CDS             complement(3195..4643)
                     /gene="gnd"
                     /locus_tag="WJL14_001763"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ7656805.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYSDTTRRTHYLAGKGILFVGAGVSGGEEGARRGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQTLLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGTDPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
BASE COUNT         1323 a         1469 c         1270 g         1238 t
ORIGIN      
        1 accgtactgg tttttgttaa tccactataa tatttcaata ctcgggacaa cgcataaagc
       61 atcacccgat gaaggttgca gaggcggaat tataagggat tttcgggaaa aatacggaag
      121 ccgcaccaaa gaatttgacg aaatgccgcg ctttccgaac aaggattgtc ggaagacaaa
      181 aaaagccgag ttttgaaaac tcagcttttt tgctttatct ggtgggtcgt gagcgattcg
      241 aacgctcgac caacggatta aaagtccgct gctctaccaa ctgagctaac gacccgataa
      301 gctcgaaatt ctatacatca agcctgatgc tgtcaaccat tttgtcggcg ttcagacggc
      361 attttattta tcaggctgtt ttttcgtata aattaacgag gtcagtatcg atgcacccaa
      421 cgcgccgaac acgaccgaca gtgaaacgga aatcgggata tgcacccaat gcattaccag
      481 cattttcaca ccgataaaac tcaacacgaa tgccaagccg tatttcagga agataaagcg
      541 ttccgccaca tccgccagta ggaaatacat cgcccgcaaa cccaaaatag cgaaaatatt
      601 ggaagtcagc acgataaacg gatcggtggt aacggcaaag acggcgggaa tactgtccac
      661 agcaaacacg acatcgctca attcaatcat gaccagcacc aaaaacagcg gcgtggcgat
      721 ttttttgccg ttttcgacgg taaaaaattt ctcgccgtga aattccgtgc cgaccggaac
      781 gactttcttg acggcattca gcagcctgct gtttgccaaa tcctctttct catcgccttc
      841 gggcttcatc atgtgtatac cagtatagag caggaacgcg ccaaacagat acagaatcca
      901 ctcaaactgc cgaaccagtg ccgcgccgac gaaaatcatg acggtgcgca ataccaatgc
      961 gcccaatacg ccgtacagca gcacgcggtg ctgaaactgc ggtgcgactt tgaagtagcc
     1021 gaatatcatc aggaacacga aaatattgtc gactgccaac gatttttcca aaatgtagcc
     1081 ggtaaagaat tccaatactt tttcttttgc gactgccgcg ccgtagccga gattgccggc
     1141 gagttcaaaa tacagccagc ccgcgaacag gcaggatacg gcaacccaca agccgctcca
     1201 tgccaaggct tctttgacgc cgactttatg gctgccgttt ttcttcagcg aaaacatatc
     1261 caaggcaatc atgaccagca ctgccgcaaa aaaaacgccg taaaacaacg gcgacccgat
     1321 gccgggatat tctgtcatgg ttcaatctcc tgatttgaaa tgtaattgtg ttaccagctg
     1381 atataaaaca tcgcttttgc caaaaagaca atcagcagca tatgggtaaa gacgacggtg
     1441 tgtatgtatt tcgaccagcc gacggtcagt gtggaacgcg ccattttgac gacggcgatg
     1501 gcgaagtgcg ccaacacgct gaacgccaac aggattttca gcgtcagcat cgtaccgaag
     1561 gaagtggcaa acggttcgcc caatatagaa agatagcggt ttgccgccat cacgatgccg
     1621 ctggcgaaca gcagtccgac cacaaacggc atcaccctga cggcgcggta agacattgcc
     1681 ttttccactt cgcgccgcgc ctcgcacgac acccgtcccg tatgcaggac ggacaaaacc
     1741 agcacttcaa aaaacacgcc gccgacaaag gcgatggcgc aatacaggtg gacgatgtgc
     1801 gcgacggcat aaatactcat acggtactcc aaccggaaaa ctcggatacg gattgtatca
     1861 ctatcgtccc cgatgtccgc ataccgcttc ccgtaccgcc tcggcgattc tcgcgcccgc
     1921 tccgcgatgt tgtgcgataa agccgtccgc gcgcgcctgc atctgcattc cgccctcctc
     1981 ggacgataag tttttttcaa cggcttcccg ccacgcgtcc gccgattcga cttgaaccgc
     2041 cgcacccgat gccaaggcgt gccggcaggc ttcggaaaaa ttgtaggtcg aaaagccgaa
     2101 tatcgtcgga acgccgcagg aaagcggttc gatgatgttc tgacagcccg aatcgaccag
     2161 actgccgccg acaaaagcga catcggcgca caggtaatac gcatacagct cgcccatact
     2221 gtcgcctatc cacacctgcg tatcgggttc gaccggcaaa ccgtcgctgc gccgctgaac
     2281 cttaaacccg aagcgtttta ccgtttcaaa taccgtctga aaatgctcgg gatggcgcgg
     2341 cacgacgacc agcagggcat cgccgcgata ttgttgccac gccgccagca gtttttccgc
     2401 ctcgtcttca ccccgataaa cgcgcgtgct gccgcacacg gcaacctgcc ggtctccgat
     2461 gcgtttttca aactgccccg ccagcgtttt catctgttcc gacggcatca ggtcgtattt
     2521 ggtattgccg cacacctgca cggatgccgc gcccaatttc gccaaccgcg ccgcatccgc
     2581 ctccgtctgt gccagacagc ccgtcagcga agcggcggca ggacggatca ggctgcggac
     2641 tttcagataa ccgtttagcg atttttccga cagccgcgca ttcgccaaaa acagcggcac
     2701 ccccgtgcgc cggcattcct tcatcagatt gggccagatt tcggtttcca tcaaaatgcc
     2761 gaacatcggg cggtgttcgc gcaaaaactg ccgtacccac gtttttttgt catacggaag
     2821 atagcggcat tgtgcatcag gaaacagaac ttgcgcggtt tcccgtcccg tcggggtcat
     2881 ctgcgtcatc agcagcggcg catcgggaaa acgctgccgc aactcgcgta tcaaaggttg
     2941 ggcggcacgc gtttccccga ccgaaacggc gtgtatccaa accgcgccgg taacgggatt
     3001 cggatacggc ttgccgaaac gctcgccccg atgcgcccga tatgcagggg cacttccgga
     3061 gcgtttgtcc aaataacgcc gtatccatat cggcgcaagc agccacaata catcataaag
     3121 ccattggaac atctttctat ttcctgcaaa acaaatgccg tccgaacggt tcggacggca
     3181 tttcggcaac ggaatcaaat atcgtaggtt gtcgaagcgg tatctccgcc cttgcccgtc
     3241 cagttggtat ggaaaaactc gccgcgcggt ttgtcggtgc gctcgtaagt gtgcgcgccg
     3301 aagtagtcgc gctgtgcctg caagaggttg gcaggcagac gttcggtcgt gtagccgtcc
     3361 aagaacgtaa tcgccgaagc catgcagggc atagggatgc cgcattcgac cgccttggca
     3421 accaccttgc gccacgccgg caggcagttt tccaaaatat ttttgaaata cggatccgta
     3481 cccaagaaca ccaaatcggg attgttttca taggcatcgc ggatattgct taagaatgcg
     3541 ctgcgaatga tgcacccctc gcgccacagc agcgcagtgt tgccgtagtc caaatcccag
     3601 ccgtagcttt cgcccgcttc gcggatcagc ataaagcctt gtgcgtagga aatgatttta
     3661 gatgcaagca gggtctgtct caacgcctcg acccattctt gtttgccgcc ttcgacgggc
     3721 gtaacggttc gggcgaacag cttgccggtc tgcacgcgct gttctttgaa cgacgaaacg
     3781 cagcgggcga ataccgcttc ggaaatcagc gtcaacggaa tacccaaatc caaggcattg
     3841 atgcccgtcc atttgcccgt gcctttttgc cctgccgtat cgaggatttt ctcgaccagc
     3901 ggttcaccgc cttcgtcctt atagcccaaa attgccgcag tgatttcaat cagataagaa
     3961 tccagctcgg ttttgttcca ctcggcaaac acgcggtgca tttcgtcgta agacagcccc
     4021 aagccgtctt tcataaactg gtacgcttcg caaatcaact gcatatcgcc gtattcgatg
     4081 ccgttatgca ccattttgac aaaatgcccc gcgccgtccc tgccgaccca gtcacaacac
     4141 ggttcgccct gcggcgtttt ggcggcaatc gcctgaaaaa tcggtttgac ggcttcccaa
     4201 gcgcgtttat ccccgcccgg cataatggac gggccgcgcc gcgcgccttc ttcgccgccg
     4261 gatacgcccg cgccgacaaa taaaatccct tttccggcaa ggtaatgtgt ccgccgtgtc
     4321 gtgtcggagt aattggcatt gccgccgtca ataatgatgt cgccttcttc caacagcgga
     4381 agcagttgtt cgataaagtc gtcaaccacc gaacctgcgc gaaccatcat catgattttt
     4441 cttggttttg ccagcttatc gaccaagtct tgcagggagt acgcgccgat aataccggtc
     4501 ccttttgccg cgccgtttaa aaattcgtcc accttaccga ttgttcggtt gtaggcgaca
     4561 accttaaatc cgcaatcgtt catattcaaa atcaggtttt gccccataac cgccaaaccg
     4621 attacgccaa tatcgccttt cattgcagga agctccgtta tagatttaat ttatcgaccg
     4681 caactctacc tgatttacac ttgtttaaca atccttaact ttttaatttt ttgaaaagat
     4741 gcctttacgc tttaccgtgc gtttccctga aggcttataa ataaaatata aaaattaaat
     4801 aaaaaaacga tgattatatt gataggagaa attttctgtg ggtaactttt ttttatttta
     4861 aaaatcatca ggattacctt tttttagggt gtcggtaagg cggattccct tttgtgcata
     4921 cctgtggatt gttttttatg gggaataagt tttgtggata gtttgcttgt tgtgcagatg
     4981 gcatcctact tttctttacc gaatggctgc cgatgtcttt aagaaccgga atactgtgga
     5041 ggtttgagag gaaagtgtgt ttggaacgtg tggaaatggt caggtgtcgg cacgaatgtc
     5101 ttatttctgc atatcggcag agtgcgcatc cgaatttgtg tataagtggt ggaaaaaatg
     5161 agatttgcgg gtaaatctca caatatttca gtcagataac tttggattgc ttgtgtataa
     5221 ataaactttc ggatggggat acgtaacgga aacctgtacc gcgtcattcc cacgaaccta
     5281 catcccgtca ttcccacgaa
//
LOCUS       DAWKQB010000140         5230 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.140, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000140 DAWKQB010000000
VERSION     DAWKQB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.140"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(461..2737)
                     /locus_tag="WJL14_001764"
     CDS             complement(461..2737)
                     /locus_tag="WJL14_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7656806.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVGLETVTVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDVDYDNPGLSCGFHDDDDAHAHTHNGRPWIDLRNKR
                     YELRAEWKQPLPGFEALRVHLNRNDYHHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYNQPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTLRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNVGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3157..3747
                     /locus_tag="WJL14_001765"
     CDS             3157..3747
                     /locus_tag="WJL14_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ7656807.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRNTMPECLEVTAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            3811..4869
                     /gene="trpD"
                     /locus_tag="WJL14_001766"
     CDS             3811..4869
                     /gene="trpD"
                     /locus_tag="WJL14_001766"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ7656808.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRYVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGRMELG"
     gene            complement(5023..>5230)
                     /locus_tag="WJL14_001767"
     CDS             complement(5023..>5230)
                     /locus_tag="WJL14_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7656809.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKFQWHLK"
BASE COUNT         1225 a         1204 c         1458 g         1343 t
ORIGIN      
        1 aaacagaaac ctttccgcgt cattcccatg aaagtgggaa tctagaaccc aaatgctaag
       61 gcgatttatc ggaaacggct gaaaccgaac gaattggatt cccgcctgtg cgggaatgac
      121 gactcattag ttacctaaaa cttaaaaaac agaaaccttt acgccgtcat tcccacgaaa
      181 gtgggaatct agaacccaaa tgctaaggcg atttatcgga aacggctgaa accgaatgaa
      241 ttggattctc gcctgcgcgg gaatgatggg tttctgcctt tgatttttct gcttttgcga
      301 gaatgacggc gtgaaagcaa gaatgatgaa acaaagaaaa tgggaatgat ggcatagtgg
      361 tttgttcttt gtctttgcca tatttcctaa caaattgatt aaaaagaaaa aaggttttca
      421 gaatgccgtc tgaaaacctt ttttgtttgc ctgtccgatt ttaaaacttc acgttcacgc
      481 cgccggtaaa gctgcggccc atttgcggcg tatcagagag gaagctgctg tgggcgtaaa
      541 cggattggtt gagcaggttg tcggctttga cgtaccaatt ccactcgcca tagcgcgtat
      601 tgcggcggta gtttgcaccg acgttgagca tatggtgtcc gggcgtgcgc gtttcgtagc
      661 gggcgagttt gttttgggcg aacacgcggt agtagtccaa gttggcatcg atacggtcgg
      721 tcagcgaggc tttcaggtgg acgccgaggc gcgcagccgg aacgcgaggg gcgttttggt
      781 cgtcctgtgc gatgaaagga cggttgccgt aggcatcttc cctaccgggt agggatggca
      841 ggtttttcag acggcctcgt acatagtcgc cggaaacgcc gatgcggtag cgcagtgtcg
      901 gtttgaagta gatttcgcct tccgcgccgt agaagtcggc accggattgg ttgtagcgca
      961 cgagcttcat ttcgctgtcg tcttcgatgg atttggggcc gcgtccgtcg tttaaggttt
     1021 gggcgtaaat gtagttgccg aagcggttgc ggtagagtgc cagattgtat tgccagcggt
     1081 cgccttcgta gcccagcgcg agttcgatgt tgttggaacg ctctttgttg aggtgtttgt
     1141 tgccgacttc aaaagtgttg gtggcaacgt gtttgccgtg tgcgtacagc tcttgcgttg
     1201 acggcaggcg ttcctgatgg gaggcggtca ggctgagttt gtgttgtggc gtgaaatacc
     1261 agttgcccga aagtgcgaac gagcgggcgg tttggcggtg tgcgccgagg tcgggcaggg
     1321 gctggttgta gtagttttcc cgatcaatca atgctttgtc gtactggatg gaggcttttt
     1381 gtttttccac gcgtacgccg ccttcaagcg tgaagttgtc ccagtttgcc tgttctacac
     1441 caaaaaagct gtaatgttgc actttattgt caagcagcat cggttgtttg accgcttcgg
     1501 atgtggcaga taaagcactg gatttttgtt gtaaatattg cacgccccaa ctgcctttca
     1561 gacggcctat aggttggtgg cgcaactcga tacgggcgtt ttgcgtttgg ttgttaaaaa
     1621 agttttctac tgcatcgcct gctttttcgt cgtggtggta gtcgttgcgg ttcagatgta
     1681 cgcgcagggc ttcaaaaccg gggagcggct gcttccattc ggcgcggagt tcgtagcgtt
     1741 tgttgcgcag gtctatccac ggtctgccgt tgtgggtgtg tgcgtgtgca tcatcgtcgt
     1801 cgtggaagcc gcagctcaag cccggattgt cgtaatcaac gtcttcttcg gtcaacaagt
     1861 gcggataaag ctgtaaatag cgtttgttaa tcaagctctt ttgccagatg atgtcggcgt
     1921 ggcaatcatc gtattcgtgg ctgtgggcag gcagaccata ttggtcgcga cggtcgctgt
     1981 acgctacgcc gataaaacct ttttcgccaa cccaagacag cccgatgctg cccgtttgcg
     2041 aatcggcgtg gctgtcgggc aggcgtttca gattgcggta acgcggtacg gcgtaatccc
     2101 ccgatttgcg gtacagccct tccgtgtgca atacaaagtt tttgcccaaa ccgatattga
     2161 tgccgccgga cgtgagtttt tccagattgc cgctgctcaa acgcaatccg agttcgcccg
     2221 atacgccgtt ttcaggcatt ttttcgggga ttttgccatc ggcaacatcg accagccccg
     2281 ccacattgcc cgagctgtac aagagcgtaa ccggcccgcg caggatttcg acctgttgcg
     2341 acaaggcggt atctaccata atggcgtgat cgggcgaaaa atccgccata tcgcctgttt
     2401 cgccgtgatg gttcaacact ttaatccgcc tgcctgtttg accgcgaata acgggagcgg
     2461 acgcgccgcc gccgtattgc gaggcgtgaa tgcccggcac gccgtccaaa gcatcgccca
     2521 agttgacggc tttttgtcgc aaggtgtcgc cgctgatgat tttgtcggag gcggtcgaag
     2581 tgtgcagcag cccggatgtg gcgcgcggac ggcttttgcc gacgacggta accgtttcca
     2641 agcccaccga ttgctcagtt tcatgcgctt gggcgaggag gggtgtgttg attaaaagaa
     2701 ttgataaaac aatgggtttg agtgtagttt gtgccatttt ggcttctcgt cgcatttcaa
     2761 aagtttgtta ttatataaca ttacattttt tatatcataa gattttgaga acactcagag
     2821 ggcataggca aaagtttttc aaatgaaacg gttgcggcat cgggcggtgt ccatttgtat
     2881 ccgccgtcat tcgggggcgc ggttgatgtt gacgcagatt ccgctgtgtt tgcccactat
     2941 gttcggctgc gggataacca aaatttatga gtgcataaaa acggcacgtt cccgaacggt
     3001 cgaaaaggtg gcaaaatggc gtacttgtca gaacgcgagg ctctgcccag gttcacgagg
     3061 gcgcatcggg cacgctaaga tataaggggt atggatggtg gtatcggaaa tgcagttaat
     3121 aacaaacaaa ttataaatca ataggttaat cacaaaatgc ttttgtttat cgacaattac
     3181 gacagtttta cttacaacat cgtccagtat ttcgctgaat tggggcagga agtcgccgtg
     3241 cgccgcaacg atgatattac gttggaagaa atcgaggcat tgaatccgca atatctcgtt
     3301 atcggacccg gcccgtgttc gcccaaagaa gcggggattt ccgtggcggc gatgcgccat
     3361 tttgccggcc ggctgccgat tatgggcgtg tgcctcgggc atcagacgat aggcgaggcg
     3421 ttcggcggca ggatagtccg cgccaaaacg ctgatgcacg gtaaggtgtc gcccgtgtcc
     3481 cattcgggca agggtatgtt taagggtttg cccaatccgg ttacctgtac gcgttatcac
     3541 agcctcgtta tcgatcggaa tacgatgccc gaatgtttgg aagtaacggc ttggactgag
     3601 gacggcgaga ttatgggtgt gcgccataag gaatatgccg tcgagggggt acaattccac
     3661 cctgaagctc tgctgaccga acacggacat gatatgttaa acaatttttt agttgaattt
     3721 caaaacttca aaccgcaaaa aatctgacgt gatgccgtct gaagcccttc agacggcatt
     3781 ttcgtccgaa tattgaacgg aggacaaaaa atgattacac cgcaacaggc tatcgaacga
     3841 ttaatcagca ataatgagtt gttttacgat gaaatgaccg acttgatgcg ccaaatgatg
     3901 agcggaaaag tgccgcccga acaaattgcg gcgattttga caggattgcg tatcaaggtt
     3961 gaaaccgttt ccgaaattac cgcagctgca gccgtcatgc gcgagtttgc gacaaaagtg
     4021 ccgctggaga atgcagaggg gctggtcgat atcgtcggta cgggcgggga tggtgccaaa
     4081 accttcaata tttcgacgac ttcgatgttt gttgccgcag cggcaggcgc gaaggttgcc
     4141 aaacacggag gccggtcggt ctcttcctcc agcggtgcgg ctgacgtgat ggagcaaatg
     4201 ggcgcaaacc tcaacctgac tcccgaacag attgcccaaa gtatcagcca gaccggtata
     4261 gggtttatgt tcgcacccaa tcaccacagc gctatgcgct atgtcgcccc ggtgcgccgt
     4321 tcgctcggtt tccgaagtat tttcaacata ttgggtccgt taacgaatcc tgcgggcgcg
     4381 ccgaaccagc ttttgggcgt gttccacacc gatttgtgcg gcatcctttc cagagtattg
     4441 caacagctcg gctccaagca tgttttggtt gtttgcgggg agggcggttt ggatgaaatt
     4501 acactgacgg gcaaaacacg cgttgccgag ctcaaagacg gaaaaatcag cgaatacgac
     4561 atccgcccag aagatttcgg tatcgaaacc cgccgcaatt tggatgaaat caaagttgcc
     4621 aatactcagg aatctttgtt gaaaatgaat gaggtgctgg aaggaagaga aggggctgcg
     4681 cgcgatatcg tattgctcaa caccgccgcc gccctgtatg ccggaaatgt cgctgcttcg
     4741 ctttcagacg gcatatctgc cgcacgggaa gccatcgatt caggcagggc caaatcgaaa
     4801 aaagaggagt ttgtcggttt tcaaccacaa caaagatgcc attttcttgg aaggatggag
     4861 cttgggtgat gccgccatga ttatggaact tttgtggcaa aacataagca cttcacgaag
     4921 agaacttacc aaactgtttt tatataaaaa cttttggggt tgtactagat aaccagacca
     4981 aattcccatt aactaattgt cttaaaatct gaatttgaga ttctatttca aatgccattg
     5041 gaatttcttt aaatgcagcc ccaaatgctc tttgggaatg ccgttaaact tacgtaaatg
     5101 gctaaattca ctaatatcaa gcacatcata actacgaaag gtatccgtgt atacaatgcc
     5161 atcaggctta actttctttc ggataattgg caacaatgtc gctgattgcg cattaggaac
     5221 aacgacggta
//
LOCUS       DAWKQB010000141         5229 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.141, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000141 DAWKQB010000000
VERSION     DAWKQB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5229
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.141"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WJL14_001768"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WJL14_001768"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ7656810.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WJL14_001769"
     CDS             785..1771
                     /locus_tag="WJL14_001769"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ7656811.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WJL14_001770"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WJL14_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ7656812.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WJL14_001771"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WJL14_001771"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ7656813.1"
                     /translation="MKHLHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAHEAGVPERMRQMFHGKKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WJL14_001772"
     CDS             4450..5001
                     /locus_tag="WJL14_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ7656814.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALLDEMAREADKKELPKRL"
BASE COUNT         1320 a         1459 c         1393 g         1057 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccgacata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 cggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagtttccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gtccatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaacgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggcatt gtggcacagg acgcgcagca taaatttttc cgtttcggca
     2281 tgtccaccgt cgcctatgtc gatacgcaca cgcagctgat ggcggcatct gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtctg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg tatcccacaa atgccgtctg
     2701 aaatgccgaa caacggtcgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacacct tcacgactta cccgcatggt
     2821 cgaaattgtg gaaccacttt gacgacagca aaacattgca tatgcgcgaa atgtttgagc
     2881 aagatccgca gcgtgcggaa cgctactggc tgcaggtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagttggcg catgaagccg
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcaaaaa aatcaatacg acggaaaacc
     3061 gcgccgtcct ccacgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gcccaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagcgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggcccgctga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccgcgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgctgt tcatcatcgc ctccaaaaca tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgacg
     3481 aagaagccgt tgccaaacac ttcgccgccg tttccaccaa tcaaaaagcc gtcgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggattg ggtcggcggt cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcattgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac acttcatcaa cacaccgctc gagcgcaacc
     3721 tgcccgtcat tctcgccctc atcggcatct ggtatatcaa ctactacggc ggcggcagcc
     3781 acgtcatcgc gccttacgac caacatttgc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag taacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ctggggcgaa acgggcatta acggccagca cgcctttttc caactgctgc
     3961 accaaggcac gcacattacc cccatcgacc tgattgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggcgc
     4141 gcatcgaaga gctggtcccg cacaaaacct tctccggcaa ccgcccgacc aacctcattc
     4201 tcatggacaa ggtcaacccg cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg tcgcaaaagc
     4381 acgacagctc gaccgaacgc ctgatcaacc tctacctgca gaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcagacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catatatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagc gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagtcgacc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 cagttctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg tgaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcgg aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttctc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgccaa agcggctctg aacagcgcgg ttcaggcagt gcccgccgat attgccgaaa
     5041 ccgttatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgt
//
LOCUS       DAWKQB010000142         5184 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.142, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000142 DAWKQB010000000
VERSION     DAWKQB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.142"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(165..1520)
                     /locus_tag="WJL14_001773"
     CDS             complement(165..1520)
                     /locus_tag="WJL14_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ7656815.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINM
                     DAVRLAGETIKRTAEITLEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(1535..1807)
                     /locus_tag="WJL14_001774"
     CDS             complement(1535..1807)
                     /locus_tag="WJL14_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ7656816.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(1933..2670)
                     /locus_tag="WJL14_001775"
     CDS             complement(1933..2670)
                     /locus_tag="WJL14_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ7656817.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(2951..3850)
                     /gene="prmB"
                     /locus_tag="WJL14_001776"
     CDS             complement(2951..3850)
                     /gene="prmB"
                     /locus_tag="WJL14_001776"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7656818.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            3995..4588
                     /locus_tag="WJL14_001777"
     CDS             3995..4588
                     /locus_tag="WJL14_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656819.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGTLTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHATSKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDGHKHYDHFILKDE"
     gene            4700..>5184
                     /locus_tag="WJL14_001778"
     CDS             4700..>5184
                     /locus_tag="WJL14_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ7656820.1"
                     /translation="MTKLYAEIAKMETQDDGTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLIDRPANPDAVSTCFKADKGAEAVNNDTEHNAT
                     Y"
BASE COUNT         1430 a         1318 c         1146 g         1290 t
ORIGIN      
        1 ggcttagacg actttttctc atagggataa ttctaactta atttgaattt ccctagttat
       61 ctaggacagc cccatattct tttctaaaga cattttaccc gatgcctata aacaaaaatg
      121 ccgtctgaag cctttcagac ggcatttccc catcaaaacc gcaatcagtt tttcatcgat
      181 tgaaccggtg cgggaatcct gccgccccgg ttgacgaaca cttcgcatga gccttctttt
      241 accggcatta caggcgcgta gcccaacagg ccgccgaact cgacgctgtc gccgacggtt
      301 ttaccggtta ccggaataat gcgcacggca gtggttttgc tgttgatcat gccgatggcg
      361 gcttcgtcgg caatgatgcc ggaaatggtg tgcgcgggtg tgtcgccggg aacggcaatc
      421 atatccaagc cgaccgaaca aacggcggtc atcgcttcga gtttatccaa cgtcagcacg
      481 cctgcttcgg cggcggcaat cataccttcg tcttcggaaa cggggataaa cgcgccactc
      541 aaaccgccaa ccgcgctcga agccatcatg ccgccctttt tcacggcatc gttcagcaat
      601 gccaaagctg ctgttgtgcc gtgcgtaccg cagacgctca aacccatttc ttcaagaatg
      661 cgcgccaccg agtcgccgac ggcaggggtc ggtgccagcg acaagtcgag aataccaaac
      721 gggatattca gcatttttga ggcttcgcgg ccgatgagtt cgcccacgcg ggtaattttg
      781 aaggcggttt tcttcacaac ttcggcaact tcggtcaatg tcgttgcatc cgaattttcc
      841 aacgcggctt ttacgacacc cgggccggat acgccgacat taatcacagc atccgcttcg
      901 cctgagccgt gaaacgcgcc cgccataaac gggttgtctt ccaccgcgtt gcagaacacg
      961 acgattttgg cgcagccgaa accttctagt gtgatttcag ccgtgcgttt gatggtttcg
     1021 cccgccagtc tgaccgcgtc catattgata ccggcgcgcg tactgccgat attgatggag
     1081 ctgcacacga tatcagtagt cttcatcgct tcgggaatgg aacggattaa cacctcgtca
     1141 gaaggcgaca tacctttttg caccagcgcg gaaaagccgc caataaaaga cacgccgatg
     1201 gctttggcag ccttatccaa agtttgcgcc acgctgacgt aagaatcagc atgggtggcc
     1261 gccgcgattt gggcaatcgg cgtgacagaa atgcgctgat tcacaatcgg cacgccgtat
     1321 ttggcagaca gatattttgc tgtcgccacc aagtctttgc cgaccgtggt aattttgttg
     1381 taaatatttt ggttcaacac gtcgatgtcg gtgctgatgc agtcgtgcaa atcaatgccg
     1441 atggtaatgg tgcggacatc gaaattctgg tcggcaacca ttttgacggt ttctaaaatt
     1501 tcgccggatt ggatactcat cacattcctc cggctcaaat gcggtgcatc gcttggaaga
     1561 tttcttcgtt ttgcatacgg atgtcgagcg cgagtttttt gctctcttcc gcaaacaaat
     1621 ccaaaacctc ctgccgcgat ttgctgcatt ttgaagtgtc caccaagata atcatggtaa
     1681 aaaaatcatc catcagctgt tggctgatat tgaggatgtt gattcggttt tccgccaaaa
     1741 ttttggaaac atcgtacacg atgccgacgc ggtctttacc gatgacggtg atgactgaat
     1801 tgttcacagg cttactcctt gcagatatcc gttaaagtcc gaaattatac caccgttgga
     1861 ttttgaagaa atattgtcaa caatatatac atacaaaatg ccgtctgaaa ctatttcaga
     1921 cagcatcaag attcagggtt cgattaaata accatcctta tcccactggg ttttcctgac
     1981 caacttatca tcctgataaa cagcttcact ctttttagaa ccatcttcat accactctaa
     2041 gactactcca ttacgtttat gatgatggat agacagttcc gagagtaatc gaccgctttc
     2101 atcccatgtc agaattttgg caggctcatc attgaccata accatttccg ttttgatatt
     2161 gccgtcggca taccattgct tccatacgcc gtttgcctta ttctgtttaa actggatttc
     2221 gctttccttg ccgccgttac ggtaatagcg gtaccccgta ccttcactca aaccattttt
     2281 ataaggcata acggcagatt ttttaccgtt cggataccag ttgacccact ccccgtccgg
     2341 cttacccttg ctgaagcccc ccgccatttt tttctgacca ttaaaatgcc acaaaatcaa
     2401 cataccgttt tgcagggtag gcacaaaaga tttgatttgc gttgaagcaa cgatataagg
     2461 ttcggaatat ttcttcatcg acggataata aaaatcctgc gcgtgcgcaa taccctccgc
     2521 cacactatat tgcctgatat aagcggcaga agacatcgtc gccgtcagtt tcccgttctg
     2581 attaaaataa acagaatagg tctgcgccgg caaagcggcc gaaaaaccca acaggacagt
     2641 tgaaaataca atccgagata attttttcat tgcaatagcg atataaaaac aaggctgtgt
     2701 tttagtaatc tgttgatttc aattatttgc aagggaaaag acaattattt tccggttagg
     2761 aataaaccta ttctattgaa tagattgaag ccaagtacgc ctatcaacac tatattaaaa
     2821 cactgccaaa aacaattaac ttataaacaa tatggtaagg atttctctgc caagcatcaa
     2881 acccgagaca acgtatcgta aaaatgccgt ctgaaaacaa atcgtcttca gacggcattt
     2941 ccctttcaac tcactcttca cccaataact gctcgcgcgt caagaggaaa acaaaaccgt
     3001 cgcccccgct ggtttccaac caagtaaaag gcaactccgg atacgctgct tccaatacat
     3061 ccctgttatg cccgatttcc accagcaata cacctttggg attcagaaac tttgccgcat
     3121 tcagaagaat ctgcctggtg gcatccaacc cgtccgcccc gctgcccaat gccaattccg
     3181 gttcgtgcaa atactcttca ggcaataact caaccgattc cgcatccaca taaggcggat
     3241 tggaaacaat caaatcataa gtgccttcca atccttcaaa caaatccgta tgaataagcc
     3301 ggatgcgttc ttccaaacca taatcttcga cattaatccc tgccacttcc aaagcatcca
     3361 agctcacatc aaccgcatca atttgggcat caggataatg atgcgccatc tgaatggcaa
     3421 ggcaaccgct tccggtgcaa agatccaacg cattatgcac caactcatcg tattctatcc
     3481 aagggcgaag tccgtcaccc aacaattcat aaataaaaga acgaggtatg attacgcgct
     3541 catccacata gaaatcaaac tctccctgcc atgcctggtg tgtcaaataa gcggctggaa
     3601 tgtgttcgac agcacgacgc tcaataaccg ccagcacttc ctctttttca gcttccaaga
     3661 gttttgcatc aagatatggg gcaagcatat ccaaaggcaa attcaaagta tgcagaatca
     3721 aataagccgc ttcatcatgc gcattatctg ttccatgacc aaaaaagagc cctgcctcat
     3781 taaaacggct gactgcaaaa cgtaaaatat cgcggatagt cgtcaattct tgtgctgcct
     3841 gattaaacat aatatgaacc attctgcgta tagatacttt taattataac agaaacaaca
     3901 agcaaacctt ttcatatcgc caaataacca cccaatctac ccatacaact acataaatgc
     3961 ccgcgcgaaa accatcgccc gaacggaaac gacaatggcc gacggtatgg gcaatctgat
     4021 tggctgggaa aaaacggggc ttgttgccgg taagcagtgg ataaccgcaa aagacgacaa
     4081 ggtgtccgat gtctgcaatg ccaacggcga gatgggcgta atcgggcttt acgagccttt
     4141 ctcacacggc acattgacga tacccggtca tccgaactgc cgatgcgagg ttgtttccgt
     4201 atcgggtggc gaattggggg aatttgccga aaaaaaggag cttcgtaaag cggctatgca
     4261 gtatgcgcgg gataacttta tcggcaaaag ctatgtcaat gaaaacagcg ggcatgaact
     4321 gaaggtaact tggcaaggtg tgaaacacgc tacgtcaaag gcaaatcagg cggaattatc
     4381 catcatgaca aaacttgatg acttattgcg ctacgcaaaa tatgagggtt cttattcgga
     4441 taggaaaggt catcctgata ttattgcagc acataagtat cgtgccgttg ccaaggttgg
     4501 gaatgagtct ttaaatatcg gtgtgattgt aagggaattt ccagacggac ataaacatta
     4561 cgaccatttc atcttgaagg atgagtaaag cccttttgca gtgtcgttct ggagcggata
     4621 gcgttaaggc aagtacgctt ccagccttac aaaagggctt taaattcagc atgccattta
     4681 tacaggcagg agtaaaccca tgacaaagtt atacgcagaa atcgccaaga tggagacgca
     4741 ggacgacggc acggtcaagg tttggggtta cgcttcaagc gaggaaatcg attcggacgg
     4801 cgaagtcatc gcggcggcag ctatgaaggc ggcgattccc gattatatga agtttggcgc
     4861 ggggcgcgag atgcacggct caaacgctgc gggaacggca attgaaatca acgttgaaga
     4921 cgacggcata acctttttcg gtgctcatat catcgacccc gttgtcgtgt cgaaagtcaa
     4981 aacaggcgtt tacaaaggct tttccatcgg cggcagcgtt accgcccgcg atgatttgaa
     5041 caagtcgcaa atcacgggct tgaagctgac agaaatcagc cttatcgacc gccctgccaa
     5101 tcccgatgcg gtgtctacct gctttaaggc ggacaaaggt gcggaagcgg taaacaacga
     5161 tacagaacat aatgctacat attt
//
LOCUS       DAWKQB010000143         5177 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.143, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000143 DAWKQB010000000
VERSION     DAWKQB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.143"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            186..1370
                     /gene="ubiM"
                     /locus_tag="WJL14_001779"
     CDS             186..1370
                     /gene="ubiM"
                     /locus_tag="WJL14_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ7656821.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(1641..1796)
                     /gene="rpmG"
                     /locus_tag="WJL14_001780"
     CDS             complement(1641..1796)
                     /gene="rpmG"
                     /locus_tag="WJL14_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ7656822.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(1828..2061)
                     /gene="rpmB"
                     /locus_tag="WJL14_001781"
     CDS             complement(1828..2061)
                     /gene="rpmB"
                     /locus_tag="WJL14_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ7656823.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(2311..3837)
                     /gene="farB"
                     /locus_tag="WJL14_001782"
     CDS             complement(2311..3837)
                     /gene="farB"
                     /locus_tag="WJL14_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ7656824.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKCRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(3861..5015)
                     /gene="farA"
                     /locus_tag="WJL14_001783"
     CDS             complement(3861..5015)
                     /gene="farA"
                     /locus_tag="WJL14_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ7656825.1"
                     /translation="MDTHTDETKLQNTQAKRKRRLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVWKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
BASE COUNT         1316 a         1456 c         1249 g         1156 t
ORIGIN      
        1 accccacacc cgatttgata cacattaagc tgaaagtaaa aatccgcata caccctcccc
       61 tgcatatttc ttcaacagtg ggttttgata taatcgccta tctgttacag atagttcaaa
      121 cggcattcaa acccttacaa ataccgtctg aaatcatcgg catccgtcta tgcaaaggat
      181 attttatgag tttacacagc gacatcctcg tcgtcggcgc aggcccggcg ggtttaagtt
      241 ttgcagccga acttgccggc agcggtttga aagttaccct gatcgaacgc agcccactga
      301 ctgttctgca aaatcctcca tatgacggac gcgaaatcgc cctgacccat ttttcccgcg
      361 aaatcatgca gcgtttgggt atgtgggaca aaattcccga aaacgaaatc taccctttgc
      421 gcgatgccaa agtgctgaac ggacgttccg actaccagct ccactttccc caaccgaccg
      481 aggcgcgcgg cgagcctgcc gactgtttgg gctatctgat ttccaaccac aatatccgcc
      541 gcgctgccta cgaagtcgta tcccaactcg acaacattac cattctgacc gatacggccg
      601 ttaaagaagt caaaacatct gacaacgaag cacaagtcat tttagaaaac ggcaaaatcc
      661 tgaccgcccg cctgcttttg gcggcagaca gccgtttttc gcaaacccgc cgacaactcg
      721 gcatttcttc agatatgcac gactacagcc gaaccatgtt cgtctgccgc atgaaacata
      781 ctctttccaa ccaacatacc gcttacgaat gcttccatta cgggcgcacc atcgcactgc
      841 tgccgctgga agaacacctg accaataccg tcattaccgt cgataccgat aaaatcaaca
      901 gcgttcaaaa cctttcgccc gaagaattgg cggcaagcgt aaaagaacag ctcaagggca
      961 ggctcggcga tatggaattg gtcagcagca tccaccatta cccccttgtc ggtatgattg
     1021 ccaaacgttt ctacggcaaa cgcagcgcgc tgatcggcga tgcggctgtc ggtatgcacc
     1081 ccgttaccgc acacggtttc aacttgggtc tgtccagcgc agatattttg gcaaaactga
     1141 tactcgaagc cgagcagcgc ggtcaggaca tcggcgcggc ttccttgttg gaaaaataca
     1201 gcagcaaaca tatgctgcat gcccagccgc tttatcacgg aaccaatatg atgctgaaac
     1261 tgtttaccaa tgagaccgcg ccggcaaaac tgctgcgcgg tttggtattg cgtgccggca
     1321 acaacttccc gccgctgaaa aaactgatta ccaaacaact gaccggttga aaactgccga
     1381 aatgccgtct gaaacgtttc agacggcatt tttgtattat agtggattaa caaaaatcag
     1441 gacaaggcga cgaagctgca gacagtacaa atagtacgga accgattcac ttggtgcttc
     1501 agcaccttag agaatcgttc tctatccttg gcagacggtt tgcagcagat tcgcatacgg
     1561 cagatgtttc aagcggaaag gaaacacgat aaaaataaca aagcctccga gcagtcggag
     1621 gctttgcttt caaactgaaa ttatttcagt ttagtttctt tatacactac gtgtttgcgg
     1681 gcaactgggt caaatttttt gatttccaat ttgccgggca tagtgcgttt gtttttggta
     1741 gtggtgtaga agtgaccagt acctgcactg gattccagtt tgattttatc gcgcattgca
     1801 gtaatcctta attaaatagt gtttaaatta agcttcgccg cgagcacgca aatcagccaa
     1861 tacgacatca atgcctactt tgtcgatggt acgcagtgca gcgttggaaa cgcgcaggcg
     1921 aacccagcgg ttttcacttt ctacccaaaa acgacgtgat tgcaagttgg gcaaaaaacg
     1981 gcgtttggtt ttgttgttgg cgtgcgatac gttgttgccg gacatcgggc gtttgccggt
     2041 cactttgcaa actcgtgcca taatcagtct ccaaacttct aaaatagtaa aacgcgattt
     2101 ataccacgaa aaacagtgtt agttcaagtg ttttaaagaa aaacggctgt tttgcgcgga
     2161 taatccttcc gattgtcgca tctgtggaac gtttgcaggt gcgaaacaga tggggcgcga
     2221 ttatagcgga tttcgggaac tgctgcattc aaaatgccgt ccgaacatat ttccagtatt
     2281 ttcagacggc atttcaagtt ttcaaatccc tcaatgtcca ccgccgccgc cgttgtggaa
     2341 cggcggtttt gccagccata tgacgggtat catgataatg aataacaagc tgcccgccat
     2401 aaagatttcg ttcgagccga taataaaacc ctgctgggta atggtattgt tgatgatgcc
     2461 tagggtttga atgtcggaaa cgccgtgctg ggacaaatga gcggccgttt cgtgcaatgt
     2521 tgcggaatag ggcgtgatgt gttcggcaaa gcgcgtgtgg tgcaacgctt cgcgccgttc
     2581 ccacagggtg ctgacgacgg atacgccgac accgcccatc agcacgcgca ggaaattcga
     2641 caggctgccg gcggcggcaa tctgcccgcc cttcatgtgc gacagtgtga tggtagtcag
     2701 cggcaggaag aacatggcaa cgcctactcc ctgccaaaac tgcggccaga tgacgttgcc
     2761 gatatccatg tcggcataaa aatcggtgcg ccaatagaaa gtgaaggcaa aggtcaggaa
     2821 gctggcggtt acgagcaggc gcatatcgat tttattgccg aacctgccga ttaacggaga
     2881 caggaagacg ggcaggatgc cgacgggcgc ggcggcaagc cccgcccacg tggaggtata
     2941 gcccaggttg gtctgcaaca ctaacggcag cagggtcagc gtccccatat acaccataaa
     3001 acccaatgat gtggcaatga tgccgacggt aaaattccga tctttaaaca gcgataaatc
     3061 gacaatcgga tatttttctc ccaattccca aacaataaaa tacgacaagc acaccagtgc
     3121 gactacgccc aaggtaatga tttctccaga ggcgaaccag tcgagttcct tacccctgtc
     3181 caacatcatc tgtaacgcgc cgataccgac taccatcaat gtaagcccga catagtcggt
     3241 cggcatttta acggtttccg tttcccgaca tttcaaatgt ttccatgtaa tccatgccga
     3301 tatgataccg atagggatat taatgaagaa aatccaaccc caatgccagt ttccggaaat
     3361 ccagccgccg agtatcggcc cgagaacagg ggcgacaacg acggtcattg cccacaatgc
     3421 cagtgccagc gtccgttttg cgggcggata ggatgccatt aacaggcttt gcgacaaggg
     3481 aatcagcggc ccggcgataa agccctgcaa gatgcggaaa acaaccagcg actgaaggtt
     3541 gggggcaata ccgcacagcc acgatgtgat gacgaaaccg acagcggcgg cggtaaacaa
     3601 tttgacctcg ccgatacgtt ttgccaaaaa gcccgttaac ggcacggaaa cggcgtttgc
     3661 cacagcaaaa gaagtaatga cccacgtccc ctgcgtggtt gccgcaccga ggttgccggc
     3721 gatgacggga acggcgacat tggcgatagt cgtatctaaa acttccataa atacggcaag
     3781 ccccaaagac agcgtaaccc acgccaatgc cgcaccctta agcggtggat aatccattgt
     3841 gtcctccgtt tcagacggca tcagggcgcg gattgcccga ggatttcatc gaccgtccga
     3901 tcgacttccg accagtcggt actttccatt tccggcaatg ccgcacccgg cgtttttgaa
     3961 acaggcgcgc ctgcggcgga agtatccact ttaaccgtca tcgacaaacc gatacgcaac
     4021 ggatgcctgt ccacatcttc gcggttcagc acgatacgga cggggacgcg ctgcaccact
     4081 ttaatccagt tgcccgttgc gttttgcgcc ggaatcagcg aaaacgcgct gcccgtacct
     4141 gccgaaaaac ctgccaccct gccgcgataa acaatttgtt tgccgtacaa atcggacacc
     4201 agctcggcag gctgtccgat tttcatatgc cgcaactgcg tctctttaaa attagcatcc
     4261 acccacacat ccgacagcgg caccaccgcc atcagcggcg cgcctgccgc cacctgctgc
     4321 ccgacctgca ccgaacgctt cgccacctga ccgtccgccg gcgcgcggat ttgcgtccgc
     4381 tgaaggttca accacgcatc tttcaacctg ccgattgccg tctgaaccgc cggctgttcg
     4441 cgcaaagaaa cctgaccgcc caaagccgca cgtgccgaag attcttccgc caaagccgct
     4501 ttgaccgccg cctgcgcctg agacactgcc gcacgggcgt gtgccagctc ttcggcggac
     4561 accgcgcccg attccgccaa agcagaccgg cggcgcaaat catcctgtgc gcgtgccaaa
     4621 tccgcccggc gcaaggcaac ctgcgccccc gcctgcgaag tggcggcatt ttgccggcgg
     4681 ttttgccgca ccgcctgaac cagctcgttt tttgcccgct cgtaagccag cacatcatta
     4741 tcgtcgtcca ataccgccag cacgtcgcct tttttcacgg catccgtatc gtcgtgcaaa
     4801 accttccaca ccgtaccgcc cttttgcggc gtaacctgaa ccacgcgtcc ggcaacataa
     4861 gcgtcttccg tttcctcttc gtgctgccac cataaaaaaa acgccgaccc ggcggctgcg
     4921 gcggcaagcg cgaacagcag cgtcaatgcc gtcaggcggc gtttgcgttt ggcttgcgtg
     4981 ttttgaagtt ttgtttcgtc cgtgtgcgta tccataagat tgggttccga atttcctgat
     5041 ttcatataaa caatgttctt tcaataggaa gcagattcta tatccgcgca ataacgcaat
     5101 caagtcagta ttttagatgc aatattcaaa aaacaacgct tcaggcggca aaactgaaaa
     5161 tatatttcgt ttatata
//
LOCUS       DAWKQB010000144         5176 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.144, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000144 DAWKQB010000000
VERSION     DAWKQB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.144"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(479..1414)
                     /locus_tag="WJL14_001784"
     CDS             complement(479..1414)
                     /locus_tag="WJL14_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ7656826.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPEAIDYVDERKGVP
                     LAAMRHILMYTEAAASHAYLFEHDLNKFKQYAYVAGKLGILRSVNSTDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYVKIASMHGFDLGIDHEIAPRDLTVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(1417..2037)
                     /locus_tag="WJL14_001785"
     CDS             complement(1417..2037)
                     /locus_tag="WJL14_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656827.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDATGKMTEQLFDSVAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSEDWIKQVANSLPD
                     GSPAKAAVFKAEREGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            complement(2116..2541)
                     /locus_tag="WJL14_001786"
                     /pseudo
     CDS             complement(2116..2541)
                     /locus_tag="WJL14_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(2589..2861)
                     /locus_tag="WJL14_001787"
     CDS             complement(2589..2861)
                     /locus_tag="WJL14_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656828.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNGMD"
     gene            complement(3049..3537)
                     /locus_tag="WJL14_001788"
     CDS             complement(3049..3537)
                     /locus_tag="WJL14_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656829.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVNFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLESH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(3534..>5176)
                     /locus_tag="WJL14_001789"
     CDS             complement(3534..>5176)
                     /locus_tag="WJL14_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7656830.1"
                     /translation="IETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPA
                     AAAVVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAA
                     GTSAAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAG
                     VLQGISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAA
                     LGAIVSTVHGEVASKIKFNLSEDYIAHKIAHAVAGCASAVANKGKCRDGAIGAAVGEM
                     VGETLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLL
                     ARRRVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAILRTPGEPWTRHDVQT
                     YRQYYNQLRESRGFAVEPIYRIRINNGNEFNRIMSSKYPYNELYVANPKSATGYFRVD
                     SYDPATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGTVISNVPSTPTTIRGRKLE
                     GKLILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
BASE COUNT         1283 a         1223 c         1076 g         1594 t
ORIGIN      
        1 tcttcctctt agcagattag gtgtacttgg aacattggaa atgacaaagt gagtgcgcaa
       61 agccatctga aggcgatgtg tttgaacctg ttgaaagcgg ccaacaggct aaatgcgccc
      121 gctgccgcct aaaaggcagc tcggatgcct gattatcggg tgtccgagga ggattaaggg
      181 ggtatttggg taaaattagg aggtatttgg ggcgaaaaca gctgaaaacc tgtgttgggg
      241 tttcggatgt cggggaaggg cttttttgca aaggtctcaa ctcatgttat tgcaaagatc
      301 tcatgccttg ttttgccagc aggttgagtt cttctccggc aatcagcgtg gtaaagcccc
      361 gccacaacac agatctatgt actgtggaag ggcttcaatt tatagctttc gaaaatcttt
      421 cagctttaag acggcctaaa gttgtaatct ataaaacagg agctcagagt tttacggctc
      481 actctcgctt cgcgttacca aataccagct tatcggtttt gaacgtatag taatctatcc
      541 aatcctgcag atattcatac ggataagaca agtcatactg tttcataaaa tcgaagatgt
      601 cttgatattc gtctgccggc agcggatcgt aaacagtcaa atccctcggc gcgatttcgt
      661 ggtctatgcc caaatcgaaa ccgtgcatgg atgcgatttt gacataggta acgatttgcg
      721 gctgcaggta gaaatcgaaa taggacaatg tttctttggc agccatacgc gcggtttttt
      781 tatcgaaaag cggctctaag gcggccttca tcgcctcggc atcctgttcg gcgaaagcga
      841 ggaagaagcg gtaatcgtac aaccgctttt gcagccattt gctaggggtg ggatgcgcca
      901 agactttctc gctacgttgt ttcaaccgat caagctgctt accctccacc atcagcagag
      961 tattgtaaat catatgacgg ttgaggtcgt atcggtttac gaaggcttct gtatcgttgg
     1021 cgatgttgtc gatattgcgc accagaaact cgcgcagctg cgggctgtcg ctcatcagca
     1081 tcagaaacat cggatcttga atgttgagca tgtcacaggg aaagaagaag ggttcagggt
     1141 ctgtagaatt tacactccgc aaaataccca gctttcctgc aacataagca tattgcttga
     1201 atttattaag atcatgttca aatagatagg catgggaagc cgcagcttca gtatacatta
     1261 aaatatgtct catcgctgca agcggaacgc cttttctttc atctacataa tctatagctt
     1321 cagggcaacc gctattgaaa ttagcagtat tgcctatgat tacattagta atatgctcat
     1381 accatttttg ggtggtcatc atattgtgcc ccattgttat ctccttatat tggttttaga
     1441 aggaactttg acagaaagaa taacggcctt acctgtttga cgatcaacgc ctgctattgc
     1501 cgtttttaat ttgccttccc gctccgcttt gaagacagca gctttagcag gactaccatc
     1561 aggtaagcta tttgcaactt gtttaatcca atcctcactc atttgcgtat atccacccgc
     1621 accattcgga ttcagctgta ccgtaccgtt cctaatctgc ttactttcta caatcaaaac
     1681 gacactacca tcggcagcct gccatacatg atcaaaaccg ttatttccgc cgtatttgcc
     1741 gcccgaaagc actctgaagc cattttgttt agctacagag tcaaataact gctcggtcat
     1801 tttacctgtc gcgtcccttc cttcgccaat agctttcagc aattgtttgt cagttgctgc
     1861 agtaaccctt aattcaattt gaccgatttt gattttggtt ttgctgacat agccctgtgc
     1921 agtggcaacg gttgctgcct tgtccaaggc ttttttcatg ccttgtaaat tttttgccgc
     1981 tttcgctact ttataggcct gtatcgattc accgataccc ggaaccacac ccagcaaagc
     2041 aaacaggtga tcggcagcgg tctgtgcttg tacaaaactc tgtatatcac cgataattgg
     2101 aataaatcct gtctgaagaa gcaaaacttc tcgatattct tcaggatttt cacgtatcca
     2161 tttttgttgt tccttttgaa attcatcaca ctttgcacca acgcatctag gatacaaagt
     2221 attattctcc accgctatct gtgccgcatt cgccgctgta ttcacatccc cgcctacaac
     2281 ggcagacgca gtgccggcaa ccagttttga ataggcagta atcttcgctt tagctttttc
     2341 gatttcggtc gcactcatac gactgaaatc agtattctta accaaagcct caccaacaat
     2401 ctcaccgact gcagcgccta tcgcaccatc ctgacacttg cccttattcg ccgccgctgc
     2461 cgcacagccc gctatggaat gggcaatctt gtgggtggta tatcgctgcc agccctctct
     2521 gttgataatc ttgcgtatga ttacgccgtt tgatggctaa tcgtacctac gcaggcttaa
     2581 aatttccatc aatccatacc atttgagttt tcatcttcat aatatggatg atcaaacaaa
     2641 tctccttccc aaatacattc actcgttata ccttgggatt ccatgaaagg aacataagaa
     2701 taaaaatctt tttcatattc tccataaaat atattcatac ctttgagcca tactgcatcg
     2761 cattcatcac acagaataat ttcatcatga agatatttag gtttcgaatg gtagagatag
     2821 ccttgttgac aagcaggaca cattttcatt ctcttattca tgattattcc atataatcaa
     2881 tttatttttg tgggtaagca atagtgattt gattgctgcc tttagtctgt acaacaatac
     2941 gtatatagca aactgataat ttcctggttt ttatcagctt tatcctcaga ttccaaaccc
     3001 aataataaaa gccgtttgaa atttttttca gacggcctta aaacttaatt aaataatgtc
     3061 cggatttaac accaaatcat attcaccaaa ctttggaaat agaattacct tatctttttg
     3121 ggaatataat tctgtttttt catatcgctc attattaata aaatcctctt tcaaaacata
     3181 gcaatcatcc gtattttcat tgaagaaata agtatatcct tctgctaagt catgagattc
     3241 caaagttata taatctctcc atacagccat agaaagaaaa taataattcg aattggaata
     3301 tttttgaaat tgatgggtta aataatccct tccaaattta tcgaaaaaat ttaccgaaaa
     3361 aaaacctact tcctctcgca aggtaattac atattcaggt tgtgtcgccg aacctattac
     3421 ggcagtatat aactcccctt taagatgttt ttcctcagct tgtttctcag aaaaaggctt
     3481 atgaagtctc ttataaccat aagaccactg ctcacaataa aaaatatctt tcttcatttg
     3541 taaatccttc ctgttgtatc tctaatgata acattttctt cttgtgccgc ccttaataca
     3601 gattgtggaa ttggattgac ctgagcagga acttctaaaa taagttttcc ttcaagcttt
     3661 cttcctctta tcgtagtagg tgtacttgga acattggaaa tgacagtacc agggctatat
     3721 tttctaacag cctccttgat ataaccaatc cccgtacttt cttggatttg agaaaattgg
     3781 gtaaattttc ttgaaataat ttcccttgtc gcaggatcat acgaatctac cctaaaatac
     3841 cccgtcgccg atttaggatt ggctacataa agctcattat aagggtattt tgatgacatg
     3901 atacggttaa attcattgcc gttgtttatc ctgattctat aaattggttc aacagcaaag
     3961 cctctggatt cccttaattg attataatat tgcctgtatg tttgtacatc atgtcttgtc
     4021 cacggctctc caggagtcct cagaatagca atcccgttaa atttcggatc cagccttcgg
     4081 atttgattgg taatggcctg gatttcaaga atggcatatt catgttccaa ttcttgtcgc
     4141 ggagtccaac gtatatttac cctcctgcga gctaaaagac tattattctc cactgccaca
     4201 gtagccgcat tcgccgccgt attcacatcc cctttaacca atgccactgc gctgcctgcg
     4261 ataatctgcg agtaggctat gactttttgg cgttcttggg gtgacagttt gcctacatcg
     4321 cgtccgtcca acagggtttc tcccaccatc tcgccgactg ccgcgccgat tgcgccgtcc
     4381 cgacatttgc ctttatttgc taccgccgat gcacagcctg ctacggcatg ggctatcttg
     4441 tgggcaatgt agtcttcgct gagattaaat ttgattttgc tcgctacttc tccgtgtacg
     4501 gtactgacta tcgcacccag tgcggcatcg cccaagttgt ctttcaggct gccgccgttg
     4561 atggcggtat ggacacttgc ggcagcggtg ctgttgatca ggttagcggt cagtttgcct
     4621 gctgcgggac tgtgaaaatg tttgctgacg gcttcggctg cttgggtgtt cagcccgctt
     4681 atgccctgca gtacgcctgc ggttacggcg gcggtggcgg cctgtctgac ggtgctgctt
     4741 ttgcccagtt ctttcagggt atggtttatg tctcctttgt tgttgatgag ggaaactgcg
     4801 gcttggctgg cgagactggc cagtgcggct ttgcctgcgg ctgtggtgat agctgcagct
     4861 gatgtgcctg ctgctactcc ggttgtggct gccgttcctg ctgctactcc agttccggct
     4921 gccgttcctg ctgcggctcc tcccgctcct gcgcctgccg cgcccgccgt tccggcggct
     4981 gccggggcgg acagtgcgcc gtaggtcaat acggttacga cgataacgac gacagctgct
     5041 gctgcgggtg tcatgccctc ctgtttgtag tcccatttat cgtaagcaag ctgcacctga
     5101 ttccagttga tgtttttcgc aacttgaagc tgttttaaat aagcatactc gggctgtttg
     5161 gtgagggttt cgattt
//
LOCUS       DAWKQB010000145         5041 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.146, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000145 DAWKQB010000000
VERSION     DAWKQB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.146"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(14..352)
                     /locus_tag="WJL14_001790"
     CDS             complement(14..352)
                     /locus_tag="WJL14_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656831.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(487..1347)
                     /locus_tag="WJL14_001791"
     CDS             complement(487..1347)
                     /locus_tag="WJL14_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ7656832.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            1505..2074
                     /locus_tag="WJL14_001792"
     CDS             1505..2074
                     /locus_tag="WJL14_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ7656833.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRVQDVETHLVVSKGAEMAR
                     ASETDYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMRRVAEMGGIVFPPVPAMYRKPQTAD
                     DVVAHSVAHALSLFGIDTPDFAEWQGTAD"
     gene            complement(2343..3209)
                     /locus_tag="WJL14_001793"
     CDS             complement(2343..3209)
                     /locus_tag="WJL14_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656834.1"
                     /translation="MKPFFKTLSAAALALILAACGGQQDSAPAASAAAPSADNGAEKK
                     EIVFGTTVGDFGDMVKEHIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHK
                     PYLDDFKKEHNLDITEAFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARALV
                     MLNELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYA
                     ISSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVSEAYNSDAFKAYAHKRFEGY
                     KYPAAWNEGAAK"
     gene            complement(3410..4096)
                     /locus_tag="WJL14_001794"
     CDS             complement(3410..4096)
                     /locus_tag="WJL14_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7656835.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVIFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRVIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(4098..4835)
                     /locus_tag="WJL14_001795"
     CDS             complement(4098..4835)
                     /locus_tag="WJL14_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ7656836.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDTGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKTRVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
BASE COUNT         1011 a         1242 c         1527 g         1261 t
ORIGIN      
        1 tccgaataaa ggattagcaa cggcctgccg ctttaagcaa agcattgcat tgacttttgc
       61 ctttgtgcgt tccgcctccc aaacaaattg catcggaagt ggtaacgccg attgtgctga
      121 ttacactggt aacatagcat tggctcacgc gcttacccac agttgcggta aagttgatgc
      181 gtatgccttc attgctgcgg ttgctgattt ttacggcatt tgggctgacg cccaaggcaa
      241 acgcggcacg ttcctgaagt ttctggtcgg aaacggttac attattgatt gagccgcaac
      301 ctgctaatgc caacgcaacg aacgcagccg aaacgatgat gcgtgtgttc ataatttcct
      361 cgaaaattaa aaatgaaaac aggaaaacga ttcttacgtg aagcagaaaa atgtcaatag
      421 aattatattt cccacttaaa atctggaaag ctattctcta tatttcagac ggtatatccc
      481 gcaaaattaa ggtcggtaat ctatgcccaa ctgcttcaag atatggtcga aggtttcggg
      541 cgtgtcgaaa tgcaggatga ttttgccttt tttatggttg gcggttttga tttcggcgtt
      601 gacacccagt ttttcagtca gcaaatcatt caggcggccg atgtcggcgg cggcagtctt
      661 tttgggctcg ggacgtttgt tttgaagggc ggcttggctg cggcgttcga cttcgcgcac
      721 cgaccagccg tttttgacgg ccttttgcgc caattcgagc tgttcgacga cgggcagggt
      781 cagcagtgcg cgggcgtgcc ccatttcgag gcggcgttgg taaagcattt cctgcacggg
      841 ttcgggcagg cttaaaaggc gcaggctgtt ggaaatcgcg cttcggcttt taccgacggc
      901 ttgggcgatg gtttcgtggg tcagtccgaa ctcgtcggca aggcgtttca agccttgtgc
      961 ttcttcgatg gggttgaggt tttcgcgctg gaggttttcg atcaaaccca ttgccaatgc
     1021 ggtttcgtcg ctgatggttt tgataacggc agggatttcg gtcagaccgg caatctgtgc
     1081 ggcgcgccaa cggcgttcgc ctgcaatcag ttcgtatcgg gacagtccgt gttcgcgcac
     1141 gatgacgggc tgtatcacgc cttgcgcctt aatcgaatct gccagctcct gcaaggcttc
     1201 gtcatcgatt tgaacacgcg cctgatagcg gccgggccgg atatctttaa ccgcaaccgt
     1261 ggtcaatcgg tcgccgctgc tgttgtctgc gccgttggcg agcagcgaat ccaaaccgcg
     1321 ccccaatccg ccttttactt ttgccatacc gccctcccgt gcctattcag ataggatgtt
     1381 aaatcgggta ttttatcgga tattgggtgt tgccgacaat ttgtatccgt gtttatcgga
     1441 tttctgtttt ttcactataa tagccggttt gccgttgcag gcggttttat gggaaaggcg
     1501 gatgatggta cggcgtttga taatcggcat cagcggggcg agcggtttcc aatacggcgt
     1561 gaaggctttg gaacttttgc gcgtgcaaga tgtcgaaacg caccttgtgg tatcgaaagg
     1621 cgcggagatg gcgcgcgctt cggaaacgga ttatgcgaga gacgaagtgt acgccttggc
     1681 cgacttcgtg catccgattg gcaatatcgg ggcgtgcatt gccagcggta cgtttaaaac
     1741 ggacgggatg ctggtcgccc cctgttcgat gcggacgctt gcctcggtcg cacacggctt
     1801 cggcgacaat ctcttgacgc gtgcggcgga tgtggttttg aaagaaaggc ggcggctggt
     1861 gctgatggtg cgcgaaacgc cgttgaacct tgcccacttg gacaatatga ggcgggtggc
     1921 ggaaatgggc ggcatcgtgt ttccgcccgt gccggcgatg taccgcaagc cgcagacggc
     1981 ggacgacgtg gtggcgcaca gtgttgcaca cgcgctgtcg ctgttcggaa tcgatactcc
     2041 ggattttgcg gaatggcagg gtacggcgga ttgaacagac gggcggatgc cgtctgaacc
     2101 gcattccgtg ttcagacggc atttgcccgt ccttgtttgc gcgtcaatcc ggacctgtcg
     2161 gtttcggtct gattggaaat ggcggcaaac ccaagcttcc ggatttctgc cttttcggga
     2221 atgacgaaag tgtgcttgcg gttcggtcgg atatcaaacc ctcttctgca aaatgcccga
     2281 tgtattcaaa aaaaatgccg tctgaactgt tcagacggca tttttcacgc accgttgccg
     2341 atttatttgg cggcaccctc attccaggcg gcagggtatt tgtagccctc gaagcgtttg
     2401 tgcgcatagg ctttgaacgc gtcggagtta taggcttcgc ttacgtcttt cagccattgg
     2461 ctgtctttgt cggcagtttt gacggcagac cagttgacat aggcaaagct cggctcttgg
     2521 aacagggctt cggtcagctt cataccgctg cttatggcgt agttgccgtt gacgacggca
     2581 aaatccacgt cggcgcggct gcgcggcagt tgtgcggctt caagctcgac gattttgatg
     2641 tttttcaggt tttcagcgat gtccgctttg gatgcggtca gcggattgat gccgtctttg
     2701 agtttgatcc aacccagttc gttgagcatc accaaggcgc gggcgaaatt ggacgggtcg
     2761 ttgggcgcgg atacggtgct gccgtctttg acttcttcca gcgatttcag cttgcccgga
     2821 tacagtccca aaggtgcggt cggcacttgg aaggcttcgg tgatgtccag gttgtgttct
     2881 tttttgaaat cgtcaagata gggtttgtgt tggaagacgt tgatgtccaa ctcgccttcc
     2941 gccaatgcca gattcgggcg cacatagtcg gtaaattcga ccagtttgac ggtgtatcct
     3001 tttttctcca gctcgggttg gatgtgttct ttgaccatat cgccgaagtc gccgaccgtc
     3061 gtgccgaaga cgatttcttt tttctccgcg ccgttatcgg cggaaggggc ggcggcagag
     3121 gctgcgggcg cgctgtcctg ttggccgccg caggcggcga ggatgagcgc gagggcggcg
     3181 gcggaaaggg ttttgaagaa gggtttcata ttttctcctg atgttgtggt ggtttcaaac
     3241 aaaaatgacg ggcagggagt cctgccgtcc ggattcggcg ttcagacggc atttgccgcg
     3301 aacaggggga ttttatagca tttttaacgg tgcggcggat atttcaccct accgtatcgt
     3361 tctcaagcct gaaaaccggc ttcatccggg cttcagacgg cattcgggtt taacgtttgt
     3421 ccaatttccg cgccaacgcg ttgccggtgc tttgaatcag gatgaccagc agcacgagga
     3481 gggcgacgat gaagacgatg acttcggttt ggtagcggta gtagccgtag cggatggcga
     3541 ggtcgcccaa gccgccgccg cctatcattc ctgctgccgc gctgtatgac aaaagtccga
     3601 tggcaagcac ggtgatgctg gaaaccatac ccgcgcgcgc ttcgtttaag aggactttgc
     3661 ggacgatggc aaccggcggc gcacccatcg cggcggcggc ttcaatcacg cctttgggga
     3721 cttcgcgcag gttttgttcc accaaacggg caaaataaaa caatcccgac acgctcaaca
     3781 ccagcgaggc ggcaaccggg ccgatggtgc tgccgacgat gacgcgtgtg gcgggtatca
     3841 tcgcaatcat caggatgacg aaggggaagg cgcgcatgag gttgactaag ttgtcgagca
     3901 ggaaatttac cggcttgttg taatgcaact ggcggctgga ggttacgaag agcagcacgc
     3961 ccagcagcgt gccgaagatg acggcgaatg tggtggacaa gccgaccatc acgaaggttt
     4021 cgcccaaggc gcggacgatt tcgcctttca taccgatgat gttggaaacg gcttgttgga
     4081 aggtcaggtc tgccatttca gtcctctctg atgagttcgc gcccgatgtc ggatcgggcg
     4141 tggatttggt tgccgcgtac ttcgacgatt tcgacgactt tgcctttatc caagagggcg
     4201 gcgcggtcgc acaggcggcg gatgacgctc atttcgtggg ttacgatgac gatggttacg
     4261 ttgaatcgtt tgttgatgtc ttccaaacat tccaagacgc tgcgcgtggt ggcgggatcg
     4321 agggcggaag tgggttcgtc ggcgaggatg acttggggtt tgggcgcgag cgcgcgggcg
     4381 atgccgacac gctgtttttg tccgccggaa agctgggcgg gatagtggcc ggcgcgttcg
     4441 gtcagtccga cgatttcgag gcattcggca acgcgcgttt tgattttttc agacggccat
     4501 ccggcgattt ccaaaggaaa ggcgacattg ccggcgacgg tgcggttgtt caacaggttg
     4561 aactgctgaa acaccatgcc gatattctgc cgcgcctgac gcaatgcggc ggcatcaagc
     4621 gcggtcagct cttgtccgca gacgttgacc ttgcccgtgt cggggcgttc caacaggtta
     4681 atcagccgca acagggtgga tttgcccgcg ccggaatagc ccatcagccc gaagatttcg
     4741 ccgtcgcgga tttcgaggct ggtcggctcg acggcggcaa aacgggtctt gtcgcgcgtt
     4801 tggtaatgct tggaaacctt gtccaaaata atcattgtct ttcccataca acaaagcccg
     4861 atgtcggaca caacgggcgc ggaagataaa gctgaaattg tcggaacgct ttagctgtta
     4921 tgcccgcaag ctgtgtcaaa tcggcaggtt aattttcgta ggatattatc gggaaagcgt
     4981 ttttttgtca ataaggcagg aagcgggcaa ccatttcgga caatatagtg gattaacaaa
     5041 a
//
LOCUS       DAWKQB010000146         4912 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.147, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000146 DAWKQB010000000
VERSION     DAWKQB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.147"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJL14_001796"
     CDS             complement(54..1538)
                     /gene="tldD"
                     /locus_tag="WJL14_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ7656837.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      1613..1716
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1783..3006
                     /gene="cytX"
                     /locus_tag="WJL14_001797"
     CDS             1783..3006
                     /gene="cytX"
                     /locus_tag="WJL14_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ7656838.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            3003..4103
                     /locus_tag="WJL14_001798"
     CDS             3003..4103
                     /locus_tag="WJL14_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ7656839.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            4124..4741
                     /gene="thiE"
                     /locus_tag="WJL14_001799"
     CDS             4124..4741
                     /gene="thiE"
                     /locus_tag="WJL14_001799"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ7656840.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLREYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTKAANPEAVVKAFQALWDG"
BASE COUNT          963 a         1520 c         1400 g         1029 t
ORIGIN      
        1 aatgtaaaag gccgcctgaa aacctttcag acggcatcgt gccggattcc gcgtcagatt
       61 gcgctgccgc cgacggtcag cccggcatcg atacgcaggg tcggctgccc cacgccgacg
      121 gggacgctct gcccttcttt gccgcacacg ccgacaccgc tgtccaacgc ggtatcgttg
      181 cctatcatcg aaacgtgttt cagcacttcg ggcccgttgc cgataatggt cgcgcctttg
      241 acggggtatt gcagcctgcc gccttccacc caccatgctt cggacgcgct gaacacaaat
      301 ttgccgctgg taatgtccac ctgtccgccg ccaaagttga cggcgtaaat gcctttgtcg
      361 atggacgcga tgatttcttc cggctcatag ctgccatttt ccataaaggt attggtcatg
      421 cgcggcatag gggcggaagc gtaactttcg cggcggccgt tgccggtgga ttgcgtaccc
      481 gtcaggcggg cattggtttc gtcctgcata tagccgacta aaatgccgtc ttcaatcaat
      541 acggtgcggc gggtttcgtt gccttcgtcg tcgatgttga gcgaaccgcg ccggccggca
      601 atatcgccct gatcgacgac ggtaacgcct ttggcggcga cgcgctctcc gatccggccg
      661 gaaaagacgc tggtcccctt gcggttgaaa tcgccttcca aaccgtgtcc gaccgcttcg
      721 tgcagcaaca cgcccggcca gccgttgccc aaaacgacgg tcatttcgcc agcgggcgcg
      781 gggcgggatt cgaggttggt gagtgcctgt ttgacggcgg catcgacaaa ctgccgcacc
      841 aagttttcat cgaaataagc caagtcgtag cgtccgccgc cgcccgcgct gccctgttcg
      901 cggcgttcgc cctgtttggc gataacggta acgttcaggc gcaccatcgg gcggatgtcg
      961 gcggcgtgtt tgccgtccag acgggcgagg taaaccatat cgtattcgca ggtcaaaccg
     1021 gccatcactt gcacgatgcg cggatcggcg gctttggcga ttgcttccac tttgttcaac
     1081 agcgcgactt tggcggcgga atcgaggccg gcaatggggt cggacgcgga acaaaccggc
     1141 ttgccgcgcg tttcagacgg cattttggcg gacaccttgc cgcctgccgc cccaatcgcg
     1201 cggacggcgc gggcggaacg gtttatcgaa tcgatgcaca ggctgtcggc gtaggcaaag
     1261 gcggttttat cgcccgaaac ggcgcgcacg cccacgccct gattgatttg gaagctgccc
     1321 gatttgacga tgccctcttc caaatgccag ctttcataag cggtgcgctg gcagtagatg
     1381 tcggcgtaat cgacgtggtg cgcgccgatg atgcacaggc ttttggcgag cagttcgggg
     1441 gaaaggcggt tggcttcgag cagccgcgcc tgtacggcgg aataggtcgg atgcatagtg
     1501 tcggcgcata aaaaatcagg ggcttgatta tacggcattt gttatggaaa tgcgccgtgc
     1561 cgccgaaaat gtaagaattt tgccaacgca cgttgctttg tgtaaactta aagctttctt
     1621 gtcggagtgc cgccgccggg cggctgagat tgcgaaagca gaatccgtag aacctgtcgg
     1681 ggtaatgcct gcgtaggaaa caaaccgtca aatgccttat caggcttccg ttcccttttc
     1741 cgcacttccc cgcccacctt ccttcttttt aaaggacgtc atatgtcggg caatgcctcc
     1801 tctccttcat cttccgccgc catcgggctg atttggttcg gcgcggcggt atcgattgcc
     1861 gaaatcagca cgggtacact gcttgcgcct ttgggctggc agcgcggtct ggcggctctg
     1921 cttttgggtc atgccgtcgg cggcgcgctg ttttttgcgg cggcgtatat cggcgcactg
     1981 accggacgca gctcgatgga aagcgtgcgc ctgtcgttcg gcaaacgcgg ttcagtgctg
     2041 ttttccgtgg cgaatatgct gcaactggcc ggctggacgg cggtgatgat ttacgccggc
     2101 gcaacggtca gctccgcttt gggcaaagtg ttgtgggacg gcgaatcttt tgtctggtgg
     2161 gcattggcaa acggcgcgct gattgtgctg tggctggttt tcggcgcacg caaaacaggc
     2221 gggctgaaaa ccgtttcgat gctgctgatg ctgttggcgg ttctgtggct gagtgccgaa
     2281 gtcttttcca cggcaggcag caccgccgca caggtttcag acggcatgag tttcggaacg
     2341 gcagtcgagc tgtccgccgt gatgccgctt tcttggctgc cgctggccgc cgactacacg
     2401 cgccacgcgc gccgcccgtt tgcggcaacc ctgacggcaa cgctcgccta cacgctgacc
     2461 ggctgctgga tgtatgcctt gggtttggca gcggcgttgt tcaccggaga aaccgacgtg
     2521 gcaaaaatcc tgctgggcgc aggtttgggt gcggcaggca ttttggcggt cgtcctgtcg
     2581 accgttacca ccacttttct cgatgcctac tccgccggcg taagtgccaa caatatttcc
     2641 gccaaacttt cggaaacacc catcgccgtt gccgtcgccg ttgtcggcac actgcttgcc
     2701 gtcctcctgc ccgttaccga atatgaaaac ttcctgctgc ttatcggctc ggtatttgcg
     2761 ccgatggcgg cggttttgat tgccgacttt ttcgtcttga aacggcgtga ggagattgaa
     2821 ggctttgact ttgccggact ggttctgtgg cttgcgggct tcatcctcta ccgcttcctg
     2881 ctctcgtccg gctgggaaag cagcatcggt ctgaccgccc ccgtaatgtc tgccgttgcc
     2941 attgccaccg tatcggtacg ccttttcttt aaaaaaaccc aatctttaca aaggaacccg
     3001 tcatgacccg tatcgccatc ctcggcggcg gcctctcggg aaggctgacc gcactgcagc
     3061 ttgcagaaca aggttatcag attgcacttt tcgataaagg ctgccgccgg ggcgaacacg
     3121 ccgccgccta tgttgccgcc gccatgctcg cgcctgcggc ggaagcggtc gaagccacgc
     3181 ctgaagtggt caggctgggc aggcagagca tcccgctttg gcgcggcatc cgatgccgtc
     3241 tgaaaacgcc tgccatgatg caggaaaacg gcagcctgat tgtgtggcac gggcaggaca
     3301 aacctttatc caacgagttc gtccgccatc tcaaacgcgg cggcgtagcg gatgacgaaa
     3361 tcgtccgttg gcgcgccgac gacatcgccg aacgcgaacc gcaactcggc ggacgttttt
     3421 cagacggcat ctacctgccg accgaaggcc agctcgacgg gcggcaaata ttgtctgcac
     3481 ttgccgacgc tttggacgaa ctgaacgtcc cctgccattg ggaacacgaa tgtgcccccg
     3541 aagacttgca agcccaatac gactggctga tcgactgccg cggctacggc gcaaaaaccg
     3601 cgtggaacca atcccccgag cacaccagca ccctgcgcgg catacgcggc gaagtggcgc
     3661 gggtttacac acccgaaatc acgctcaacc gccccgtgcg cctgctacac ccgcgctatc
     3721 cgctctacat cgccccgaaa gaaaaccacg tcttcgtcat cggcgcgacc caaatcgaaa
     3781 gcgaaagcca agcacctgcc agcgtgcgtt ccgggctgga actcttatcc gcactctatg
     3841 ccgtccaccc cgccttcggc gaagccgaca tcctcgaaat cgccaccggc ctgcgcccca
     3901 cgctcaatca ccacaacccc gaaatccgtt acaaccgcgc ccgacgcctg attgaaatca
     3961 acggcctttt ccgccacggt ttcatgatct cccccgccgt aaccgccgcc gccgtcagat
     4021 tggcagtggc actgtttgac ggaaaagacg cgcccgaacg cgatgaagaa agcggtttgg
     4081 cgtatatccg aagacaagat taaagccgtc tgaaaggaac cccatgacct tcctgccact
     4141 caaatccccg ctcaaattct acgccgtcgt ccccaccgcc gattgggtcg agcgcatggt
     4201 cgaagcaggt gccgacacgg tgcaactgcg ctgcaaggcc ctgcacggcg atgaattgaa
     4261 acgcgaaatc gcccgctgtg tcgccgcctg tcagggcagc catacgcagc ttttcatcaa
     4321 tgaccactgg cgcgaggcaa tcgaagcagg cgcgtacggt gtgcatctcg gacaagaaga
     4381 catggacacc gccgaccttg ccgccatcgc cgccgccggt ttgcgcttgg gtttgagtac
     4441 gcactccgtt gccgaactcg accgcgccct gtccgtacac cctagctaca tcgccagcgg
     4501 cgcgattttc ccgaccacga ccaagcaaat gcccaccgcc ccgcaaggct tggacaaact
     4561 gcgcgaatac gtcaaacagg caggcggcac gcccgtcgtc gccattggcg gcatcgattt
     4621 gaacaacgcc cgagccgtac tcgccaccgg cgtttcctca ctcgccgccg tccgcgccgt
     4681 aaccaaagcg gcaaatcccg aagcggtggt taaagcgttt caggctttgt gggatggata
     4741 aaaccgaaag aagaaaattc aattgccgtg taggcaaaac ttagcccgtt atcgcaaaca
     4801 tacttaactt taaatgtggc atatcatcaa attccgtcat ttccgcgcaa gcgggaatcc
     4861 gccttaaaac ttgagaaacc atcatttgaa aaacagtttc cgaatttcaa aa
//
LOCUS       DAWKQB010000147         4832 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.148, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000147 DAWKQB010000000
VERSION     DAWKQB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.148"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(92..574)
                     /locus_tag="WJL14_001800"
     CDS             complement(92..574)
                     /locus_tag="WJL14_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ7656841.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDISCGC
                     "
     gene            complement(727..888)
                     /locus_tag="WJL14_001801"
     CDS             complement(727..888)
                     /locus_tag="WJL14_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656842.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            complement(962..1423)
                     /gene="recX"
                     /locus_tag="WJL14_001802"
     CDS             complement(962..1423)
                     /gene="recX"
                     /locus_tag="WJL14_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ7656843.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(1491..2198)
                     /locus_tag="WJL14_001803"
     CDS             complement(1491..2198)
                     /locus_tag="WJL14_001803"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ7656844.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            2605..3870
                     /locus_tag="WJL14_001804"
     CDS             2605..3870
                     /locus_tag="WJL14_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ7656845.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGITLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEALFKEFQEKGFITNE
                     AGYGKEITNAELLALDVDVLAPCALENQLTSENAGKVRAKIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(3928..4734)
                     /locus_tag="WJL14_001805"
     CDS             complement(3928..4734)
                     /locus_tag="WJL14_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ7656846.1"
                     /translation="MKKTLTLLAVSALFAASAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKQMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTT
                     DGKGEVDIIPLRQGFWKASVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
BASE COUNT         1040 a         1104 c         1430 g         1258 t
ORIGIN      
        1 aatttaatcc actatatttg cttgatttca tccgtttgca atggaaacgg atggcgcgat
       61 gtgcctgtct ttcagacggc atagtccgcc gtcagcagcc gcaggagatg tcttcagagg
      121 cttgcaggct gatgtctctg cgttttttgg ctttttcgta gcggcagcgt tggcggtcgg
      181 tcaggatttc cagcggaggg acagggacgg gtttgccgtc tttgaccgca accatcgtga
      241 agtagcagct gttggtatgg cggatttcgc cggtgcggat gttttgtgct tcgacacgga
      301 tgccgatttc catcgaggtg cgtcccgtat agttgacagc ggcgtaaaag gtaaccaaat
      361 cgccgatgtg gatgggttct ttaaacagga ctttgtcaac cgacagggta acgcaataat
      421 tgccgctgta acggctggcg caggaatagg cgacttggtc gagcaggagc aggagttcgc
      481 cgccgtgtac gttgccgctg aaattggcgg tgtccggcat catcagttcg gacataatga
      541 gttcgtgcga aggcagttgg cgttgttgtg tcatggtgta cctgttttaa gaaagggtgt
      601 tttgaaatgg gtgcgcgaaa attttaggga atggggaagc ggaattcaag ctaaaaaaag
      661 ctatggcggg atagtctgca gaaatgggcc gccgatgccg tctgaagcct tcagacggca
      721 ttttgtttag gaaagccaaa aacggaagac caggaaaaac acgagtgcca atacgcccgc
      781 ccaaacgacg agcatgaatc cagcaactgc ccgtcccata cggtaaaccg ggtttttgcg
      841 ttcgtcttcg atgggggagg tgtggtgttt ttcgggttgt ccggacattt tgttctcaat
      901 cggaaaagtt ggaaacgggc gtaaggttat gttgccgtgc aaaaagattc aagaaggaag
      961 gtcagcagcc gtcttcccaa ttttcgtccc aagcgtgttt caatgccgtc tgaacggtat
     1021 cggcatcgaa tccgcgatag gcgaggaaac gcgcctgttt ttgtttttct ttgaggtcgg
     1081 cggcggggtg tttgaatttt ttacgcaaca cggctatggc ggccagtttt tcgcttgagc
     1141 ggtcgggaag caggttgcgg ctggtttctt catctatgcc ctgttgcgcc aaagcctgtt
     1201 tcagcctcaa tgaaccgtgt ttgcggcttt tgctgcggat ataggcttcg gcatagcgca
     1261 aatccgactg ccagttgcgt tcggcaaatt cgtttaacac gttttccaac tcctcttcgc
     1321 tttcggcgtg cggtgcaagt ttgcgtttca gaccgatgcg gctgagttct tggcgcgaga
     1381 ggatgtccat cgcgcgggcg cgtagggatt tttgcggttt catggcggcg gtttcggcgt
     1441 ggggcaggtt tcagacggca tagcggcgca aaccggagcc gtccgaatgc ctactcttct
     1501 ttgtttttct gaggttgcag gttttcgtaa atttcgggca gcgagccgat aatccagtcg
     1561 gggcgggtcg catcgtcttg cgagagcagc gtcatatcgc cgtaaccgaa ggtaacgccg
     1621 acgctcaggc agccggcggc tttggcggcg atgatgtcgt tgcgcgagtc gccgaccata
     1681 accatgtttg caacatcgat acccaaaact tcggcggcgt gccgcagcgg cagggggctg
     1741 ggttttttct cgggcaggct gtcgccgccg agtatcaggc tgaagtagtc ggcaagtccg
     1801 agctgtttca acagttcgac ggcaaggatt tcgtttttgt tggtaatgac tgccagcggg
     1861 atgcccaaag atttgagcaa tgccagcccg gcttcggttt cgggataggg gcgggtgaag
     1921 acgctcaaat ggtcgcggta gtatttcata taggatacga aacctttttc ccacagttcg
     1981 gaatcggctt cgcggtcgcg gtcgttggag aggacgcggt gaaccagttt gccgatgccg
     2041 tcgcccacat agctttcgac cactttggcg ggcagcggtt tcataccgag ttgttccaac
     2101 atcgcttctg cggcggcggc aaggtcgggg acggaatcgc acagtgtgcc gtccaaatcg
     2161 aaggcgacgg cttggacgtg ttcgatggct gcattcataa tctgatttcc tgtcgggttg
     2221 aaagggttta ttttaacatc gtttgcacag ggcttctaat gtatagattg ttaacaatcg
     2281 caacaggttt tgcaggcggt gttttcggat ttcggggcgg aattgggcaa gggtttgaaa
     2341 tcccggcgat gtgccaatta aatttttaaa aaataaaagg atgcctatat ctttatttaa
     2401 ttggacattt atttttcgga cgcgcgcgct gctttttcag gcggagtccg ggctgattcc
     2461 gtgcggcttg aaaaataaaa tcatatatag attaatcaaa tcgggcttat gccgactaca
     2521 cgctattaca ttttgtcggg aaattggtta tagtaatgag cgaaatgcac gcccctgaaa
     2581 atctttttgt gaaaaggaag caaaatgtct gaagcccttg caaaagaaac cctcaatccg
     2641 tttgaaatcg cgcgcaaaca ggtcaaaacc gcctgcgacc ggctgaaaac cgatccggcc
     2701 gtttatgaaa tcctgaagag tccgacccgc gtgttggaag tcaatttccc cgtcaaactc
     2761 gacgacggca cggtcaaaac cttcacgggc taccgttcgc aacacaacaa cgccgtcggc
     2821 ccctataaag gcggcgtgcg cttccatccc ggtgtgaatc tggacgaagt caaagccctg
     2881 tcgatttgga tgaccatcaa atgctgcgtg gccggtattc cttacggcgg cggcaaaggc
     2941 ggcattacct tggatccgcg cgattattcc gaagcggaac tggaacgcat cgcacgcgct
     3001 tattccgaag ccatttctcc gctcatcggc gagaaaatcg acattcccgc acctgatgtg
     3061 aacaccaacg gcaaaattat gtcgtggatg gttgatgcct atgaaaacgt agtgaaacat
     3121 tctgcaccgg gcgtatttac cggcaaaccg gttgagttcg gcggctcttt ggcgcgtacc
     3181 gaggctaccg gttacggcgt gaacctcgcc gccgtccaag ctttggaaaa actgggcaag
     3241 gacgtgaaag gcgcaaccta cgccatccaa ggcttcggta atgtcggtta ccacacaggt
     3301 tattacgcgc atcaatccgg cgcgaaagtc gttgcggttt ctactgttga cgttgccatt
     3361 tacaacgaaa acggcttgga tatggaagct ttgtttaaag agttccaaga aaaaggcttc
     3421 atcaccaacg aagccggtta cggcaaggaa attaccaacg ccgaactttt ggctttggat
     3481 gtggacgtac tcgccccttg cgcgctggaa aatcaactga cttctgaaaa cgccggtaaa
     3541 gtccgcgcga aaatcgtggt cgaaggcgcg aacggcccga ctacgcccga agccgacgtt
     3601 atcctgcgtc aaaacggcgt attggtcgtg cccgatattt tggcgaactg cggcggcgtg
     3661 gtggtatctt attttgaatg ggtacaaaat ctgcaaggct attattggga gtttgacgaa
     3721 gttcaggaaa aagaaaccgt cgtcctgcgc cgtgcgttcc gcgatatatg gaacttggcg
     3781 caagagtatg atgtcgattt gcgaaccgcg tcttacatga tgagtatccg ccgtgttgaa
     3841 aaagcgatga aactgcgcgg ttggtattga tgtttgaaca aaaaatgccg tctgaagcct
     3901 tcaggcggca tttttgtgcg ggcgggatta atggtgagaa tggccgattt ggaaggttaa
     3961 agttgagtag ttcgcctgtt tttggcacac gctttgatcg gggaagtcgg ctttgtgttc
     4021 gacactggct ttccaaaagc cttggcgcaa ggggatgatg tccacttcgc ctttgccgtc
     4081 ggtggtgtcg gagaaagcct gtgcttcggt tttgtgcgtt ttgctgcggt cgctggtgtc
     4141 gaagccgtca aaggtggcgg taacggtggc attgggcagc ggttcgccac ggaacagaac
     4201 gcggactttg aagcgttcgc ctacgtgaat gttggcggga ttgtccagcg ggacgatttc
     4261 caagttttgt ccgaccggtt tggtgatgat ggcggtgtcc gcgctttcgt gtccgacgtt
     4321 gacgatgttt ttgccgaaca ttcgggtttg ttcgcaatag cttgcgtcag gcatttgttt
     4381 gatgcccgcc tgtttccagc ctgctttgtt ttttgaccag aaagtaggct gatattcggc
     4441 gatgacgagg taactgccgt ccttaacggg acggttgctt cggtactggt agttgtatgt
     4501 gccgcgttga atcatgtttt ccttgccttt ttcggtaacc agctgcatcg gtttgctgaa
     4561 gatgtgcagg cggtctttgg cgatgggttc gagttcggga aattcgccgt agcccaagtc
     4621 ggctttaagg tattcgccgc cgtgcgtgtg ggcggtttcg acccagacgc ggtgggcgtg
     4681 ggcggatgcg gcaaataggg cggaaacggc gagcagtgtc aatgttttct tcatggtgca
     4741 gcctttgtct cgtgtgggtt aaagattttg ttatgttata acaaaatagg tatcgtgtga
     4801 agacggattt gctgtctgaa aactgtcttt tt
//
LOCUS       DAWKQB010000148         4773 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.149, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000148 DAWKQB010000000
VERSION     DAWKQB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.149"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            107..1615
                     /gene="nadB"
                     /locus_tag="WJL14_001806"
     CDS             107..1615
                     /gene="nadB"
                     /locus_tag="WJL14_001806"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ7656847.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(1793..2458)
                     /gene="pgmB"
                     /locus_tag="WJL14_001807"
     CDS             complement(1793..2458)
                     /gene="pgmB"
                     /locus_tag="WJL14_001807"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ7656848.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDAVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(2472..4730)
                     /locus_tag="WJL14_001808"
     CDS             complement(2472..4730)
                     /locus_tag="WJL14_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ7656849.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINAFNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMRHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGTLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHTVALEK"
BASE COUNT          960 a         1353 c         1379 g         1081 t
ORIGIN      
        1 caccttagag aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttaaattt
       61 aatccactat accgtttcag acgatctctc aaacaaggac aacaccatgc aaaccgattg
      121 cgacgtattg attgccggaa acgggctggc ggcactgacg ctcgccctgt cgctgcctga
      181 atcgttccgc atcgtcattt tgtgcaaaaa ccggctggac gacactgcca gccgtcatgc
      241 gcaaggcggg attgcggcgg cgtggtcggg agaggacgac atcgaaaaac acgttgccga
      301 taccttggag gcgggcgcgg gtttgtgcga cgaagccgcc gtccgcacca tcctgtcgca
      361 gggcaaactg gcaatcgaat ggctgctggc gcagggcgtg gcgttcgacc aaaataataa
      421 cggcctgcac ctgacgcgcg aaggcgggca cacctgccgc cgaatcgccc acgtcgccga
      481 ctacacgggc gaagccgtca tgcagagcct gactgtccaa atacgctgcc gcccgaacat
      541 ccgcgtttgc gagcagcaga tggcgttgga catccaaacc gaatcaggtg cggcgtgcgg
      601 actgaccgtc ctcgactgcc gaacgcaagc aacctacagc atccgcgccc gccataccgt
      661 actcgcaggc ggcggcttgg gacagattta cgccgccacc accacgccgc ccgaatgcac
      721 gggcgacgcc atcgccatgg cgatacgcgc aggctgcgca gtcgaaaacc tcgaatttat
      781 ccaattccac cccacaggct tggcaagatc gtctgaaaac ggccgcacct tcctgatttc
      841 cgaagccgtg cgcggcgaag gcggcatcct gaccaaccaa gcgggcgaac ggtttatgcc
      901 gtattacgac cgccgcgccg aactcgcgcc gcgcgacatc gttgcccgcg ccatcgccgc
      961 cgaaatcgcc aaacaaacgc aagacttcgt ctcactcgat atcagccatc aacccgcagc
     1021 gttcgtccgc cggcatttcc cgtccatcca tcggcactgt ttatcccaat gcggcctgga
     1081 tatcacgcgc caagccatcc ccgtccgccc cgtgcaacac tacacctgcg gcggcatcca
     1141 aaccgacccc tgcggcagaa cttccctgcc gcagctctac tccttaggcg aaaccgcctg
     1201 caccggcttg cacggagcca accgccttgc cagcaactcc ctgctcgaat gtgtcgttac
     1261 cgccagactt gccgcccaaa ccatcgcaga cggacaagca ttccaagcca aaacgtctcc
     1321 aaggccgtct gaaagcctct cctccgaagc aggcatcttt tcagacggca tccaaagcgc
     1381 attcagccgc cccgtcctgc aagcgttcaa ccaacgccat ctgggcatcc tccgcaacga
     1441 taccggtctg cgctgcgcca tcgcccagct gcggctttgg aaacaaaacc aaaccgaacc
     1501 gcacaccgcg tccgaatacg aaaaccgcaa cctgctcgaa tgcagcctcg ccgtcgccca
     1561 agccgcatac aggcggagac agaacatcgg cgcgcatttt aatagtgatt gttaaggttg
     1621 gctggttaga aaaaaaatcg ttcaaataaa tttgaatagc atggttaaga aattggcatt
     1681 tagtttttag ggttgcgtaa gttgggttat caatctgttc taaaagcaga aaggccgtct
     1741 gaaacactgt tcagacggcc ttttacttcc ttgacacttt atctgacgcg ttttacctgc
     1801 ccgactgtgc ccacacgttt tgcaggtagg cgtaggtcag ctcggcggta tcggacacca
     1861 gcgcgatgtc gctgcccaag tcttcggctt tgcccacgcc gataggcaag gcgccggcgg
     1921 ctttgatggc ggcgacacct gcggcagcgt cttcaatgcc gatgcagcgg cggatgtccg
     1981 cgcccacgcc ctcggcggcg gcgaggaaga tgtcgggggc gggtttggaa tgcgcgacgg
     2041 cggcagggtc ggcgacggcg tcgaagaagt gggtcagccc catgcgttcg agcaggaacg
     2101 ggccgttttt actggcggac gcgagggcga tttttttgcc gtttgctttt aatgcttcca
     2161 gcagcggcaa aatgccgaga tacacgtctt cgggtttgac tgcctgaatc atctcgacgt
     2221 agttgtcgtt tttgcggcgg gtcagttcgg cgaactcggc ttcgccgacg gttttgccgc
     2281 cgtgtgcgag gatgcgtttg agcgaatcgt cgcgcgatac gcctttgagc tgctcgttaa
     2341 acttgcggtc gatgctgatg cccagttctt cggcgagctt tttccatgcg cggtagtggt
     2401 attcggcggt gtcggtgatg acgccgtcga gatcgaagag cactgcggta aacgtcattt
     2461 ttgcgccctc cttatttttc caacgcgaca gtgtggctgc cgtcgagcgt gatgtctttg
     2521 ccgtacacct gcaaatcgag cggctcgcct ttgagcaggg tgaagacgac gttttctttg
     2581 ccgacggcga ctttaatcag acggcctcgg tagttgatat ggaaggcata gcctgtccat
     2641 gcgctcggca ggaacggtgc gaagctgagt gtgccgcccc aagttttcat ttgggcgaaa
     2701 ccttggacga tggcgagcca cgagccggtc atggaggtga tatgcaggcc gtcttctgtg
     2761 tcgttgttgt agttgtccaa gtccaggcgg gcggtgcgcc ggtacatttc cacggctttt
     2821 tcttctttgc ccagttcggc ggcgagaata gagtggatac agggcgacag cgagctttca
     2881 tgcacggtca tcggttcgta gaaatcgaag ttgcggcgtt tttcgtcgat attgaaacgg
     2941 tcgccgaaga agtagatgcc ttgcaacacg tccgcctgct tgatgaaggg cgaacgcagg
     3001 attttgtccc acgaccattt ctggttgagc ggcaaatcgt cgggcgaaag cgcggacacg
     3061 ggacggatgt ctttgtcgag gaagccgtcg tgttggacga atacgccgag ttcttcgtca
     3121 tgcggacggt acatattcgc gctgatgtcc gcccattttt ccaactcggc ggcacgcacg
     3181 ttcaaatccg ggcgcgggta tttcgccagg gcttcgcggg tgtagtccaa tacccaggcg
     3241 gcgagggtgt tggtgtacca gttgttgttg atgttgtttt cgtattcgtt cgggccggtt
     3301 acgccgtgaa tcatgtattt gccgttgcgt ttggagaagt ggacgcggtc cgcccagaag
     3361 cgggacactt cgaccaagac ttccaagcct tctttggcaa gatagccctc gtcgccggtg
     3421 tagttggtgt agttgtagat ggcgtaagga atcgcgccgt tgcggtggat ttcctcgaag
     3481 gtgatttccc attcgttgtg gcactcgata cccgtaaacg ttaccatcgg atagagtgcg
     3541 cccgccaagc cctgttcgcg cgcgttgtgc tgcgcctgcg gcagttggtt gcggcggtat
     3601 tgcagcaggt tgcgggtaac ttcgggttcc gccagtgcga ggtagagcgg tacggcatag
     3661 gcttcggtat cccaataggt cgcgccgccg tatttttcgc cggtaaagcc tttggggccg
     3721 atgttcaggc gcgcatcttc gccgtagtag gtggagaaca gttggaacag attgaaacgg
     3781 atgccttgct gcgcttcgtc gctgccttcg atgaccacgt cggcgatttc ccaacggtgc
     3841 agccagcctg ctttgtgcgc gtccagcaag gtttcaaacg caacgcctgc gactttttcc
     3901 gacaaggcgc ggcctgcggc tttcactgct tccaagccct gataatcgcg gctggtggta
     3961 acaataaccc gtttttcaaa ggttttgggc gtgccgccga cttcgacctc gaaagaattg
     4021 gagacttgcc agtcggtttg gctgccgccg agggctttga agctgccggc gaaagtttgc
     4081 tcggcgttga cgataaattg ttccacgccg aaagggttgg caacggtttg ggcggcaatg
     4141 taggagagac tgtctgaaac gcctttgtcc aatacctgcc agaatttttc ttcgtagttg
     4201 gagtcttcgt ttttcacgtc ggcatcgatg atggaatcga tgcggacttg gtgggtttta
     4261 ccgtcaacgg atacggcttc ccagcggatg accgccagct ctttttgcgc gacggacagg
     4321 aatttgcaca cattgaaacg cacaccgaat acggtaaacg agcggcgcaa cacgccgtgc
     4381 cgcatatcga gttcgacgga gaagccggca acgtcgtttt tcgccaagtc cacttcctgc
     4441 ccgtcgacaa agattttgac tttgctgaaa ttgaacgcgt tgatggcttt gccgaaatat
     4501 ttgggatagc cgtttttcca ccagccgacg cgggttttgt cggggaacca cacgccggcg
     4561 atgtaggtgc ctaagtggct gtcggcggaa taggtttcct caaagctgcc gcgcataccc
     4621 atatagccgt tgcccaagct ggtcaggctc tcttgcagcc gtttgtgttc tttttccagt
     4681 tttgccgaac gcagcgtcca agggctgatt tccatgattc ttgtgtacat ttatgaagct
     4741 cctgtttgga ttgatttgag ggaatggtga aat
//
LOCUS       DAWKQB010000149         4769 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.150, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000149 DAWKQB010000000
VERSION     DAWKQB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.150"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(471..1007)
                     /locus_tag="WJL14_001809"
     CDS             complement(471..1007)
                     /locus_tag="WJL14_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ7656850.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1041..1397)
                     /gene="folB"
                     /locus_tag="WJL14_001810"
     CDS             complement(1041..1397)
                     /gene="folB"
                     /locus_tag="WJL14_001810"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ7656851.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1453..2055
                     /gene="plsY"
                     /locus_tag="WJL14_001811"
     CDS             1453..2055
                     /gene="plsY"
                     /locus_tag="WJL14_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ7656852.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPTTALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGEKR"
     gene            2143..2988
                     /locus_tag="WJL14_001812"
     CDS             2143..2988
                     /locus_tag="WJL14_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ7656853.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     AVLIDPPYEEKQDYWRVTETLKSALKRFESGCYLIWYPCLSREESRKLPEELKKLVPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            3234..4208
                     /locus_tag="WJL14_001813"
     CDS             3234..4208
                     /locus_tag="WJL14_001813"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ7656854.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(4367..4585)
                     /locus_tag="WJL14_001814"
     CDS             complement(4367..4585)
                     /locus_tag="WJL14_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ7656855.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         1224 a         1401 c         1018 g         1126 t
ORIGIN      
        1 gagctaaggc gaggcaacgc catactggtt tttgttaatc cactataccg ttctcaaatt
       61 caaattgggc ttttaggtct ataacaaaga aaaagccttg gcaggataaa cctaccaagg
      121 ctttttatta aaaaaaacgc tctattactg tgtctaaaaa tgcaaaaaaa gtaaccatca
      181 taaccctttt gccaaaatca atatcatctt ccgatgaatg aattgacggt atataagaaa
      241 gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagattttc attattaacc
      301 attcttaaaa atgatcacgt tcgtttgggt aattattata cccttttccg tcttattact
      361 aaagcacgaa tgactttaaa ggacgcgccc agagacaagc cgaaaaggca tatctcatcc
      421 gccaaacaaa atgcccgcca atctactccg gagggcagtt caacaccctg tcaatccttc
      481 atcagccagt attgcaaacc gattaatgtc ttgccgtctt taatttcatc gtttgccaat
      541 gcctgacgga cttcttcttt cgacatcaat acggtttccg taatctcgtc ttcgtcattg
      601 gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca gatacatttt ttcgttgcaa
      661 aaaccgaccg ccgtataaaa actgtaaagc aggcgtacgc tgtcggtggt ataaggcgtt
      721 tcttcagcca gttctcgtaa agcgcatgct gccatatcct cgcccgccac atccagcttg
      781 cccgcaggaa gttccaatgt cgcctgattt gccgcatacc gccactgccg taccaaaacg
      841 accttttcct catccgttac cgccaacacg cacgccgcgc ccggatgccg gatgacgata
      901 cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatgct gacgaaaccg
      961 ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc atatctcctt
     1021 tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc cacgccaacc
     1081 tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca caccgcgccg
     1141 aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag caggaaatcc
     1201 tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac cgtattggca
     1261 atatcgtcgt ccgaacccgc tttctcggga acgccgatgt ccaaatcgac aatcagggtc
     1321 tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt gatgccgtgc
     1381 aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac cattctaagt
     1441 aaagtaccgc aaatgttcaa cataccggct gttgccgttt cctatctgat cggctcactt
     1501 tctttcgccg tcatcgtgtc caaatattac ggcatggacg acccgcgcac ctacggatcg
     1561 ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc cgcgctgacg
     1621 ctcttgggcg atgccgccaa aggtttggtt gccgttttgc ttgcacgcgt gcttcaagaa
     1681 ccgctcggtt tatccgacag cgcaatcgcg gccgtcgcac tcgccgcgct ggtcgggcat
     1741 atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc attgggcgtg
     1801 cttctggcac tctctcccac aactgccttg gtctgcgcgt tgatttggct tgtgatggca
     1861 ttcggcttca aggtgtcctc ccttgccgca ttaaccgcca caatcgccgc cccccttgcc
     1921 gcactgtttt ttatgccgca tacttcttgg attttcgcaa ccctcgcaat cgccatattg
     1981 gtgttgctcc gccataagag caacatcctc aacctgatta aaggcaaaga aagcaaaatc
     2041 ggcgaaaaac gctgattcgg catttgtttt ccattacgtc atgccgtctg aaaccatgtt
     2101 cccgcgttca gacagcattt ccgcacaaac ggctttcata ccatgctcag ttacagacac
     2161 gcattccacg ccggcaacca cgccgatatg ctcaaacact ttaccttgtt tttagtgctg
     2221 caatatttca accgcaaaga caagccctac tggtacatcg acacgcacgg cggcgcgggt
     2281 gtgtacaatc tcgaaggcag cgaggcgcag aaagtcggcg aataccggca aggcatcgcc
     2341 ctgctccgac aggcgcaaaa cctgcctgcc gaactctctg actttgccgc gcacatacaa
     2401 aaaatcctgc cctcgcccga actttactgc ggttctccgt ggctggcaca atcgctgacc
     2461 cgcgtcggcg acaaattacg cctgtttgag ctgcacccca ccgactttgt ccatctgcaa
     2521 aacaatatgg gcgaagcagg attgggcaaa cgcggacaag tgttgcgcga agacggttac
     2581 aaagggctga tttccctgct gccgcccccg ccgcgccgtg ccgccgtcct catcgacccg
     2641 ccttacgagg aaaaacagga ctactggcgt gtaacggaaa cgctgaaatc cgctttaaaa
     2701 cggtttgagt ccggctgtta tctcatctgg tatccctgcc tcagccgcga agaaagccgc
     2761 aagctgcccg aagaactgaa aaaactcgtt cccgacaact accttcacgc cgaactgcac
     2821 gtccacgcgc cgaaagccga cggtttcggt atgcacggca gcggaatgtt cgtcatcaat
     2881 ccgccctatc tgttggcgga acaattggcg gcaaacctcc ccgccctgac gcggctgctg
     2941 gcgcaagacg aaggcgcgcg ctaccttttg gacagcaaaa tccgctgacc ccgatgccgt
     3001 ctgaaaccct tcagacaaca tctcccgtca aataaacaaa accggacgat ataccggcag
     3061 tcggcacttt taagaataaa tatcccctca tccgcctgcc gcgttccgac ttgccccatg
     3121 ccgttttttt acactacaat cccctagact atcctgcaaa acgcaccaaa ggaatacgcc
     3181 acaataacct gccgcctttc agacggcaca aacctttccg aaacgagcaa actatggaca
     3241 cactgacccg attcctcccc gaacacctgc aacaaaacca gctgcccgaa gcactcggcg
     3301 gcgttttact gtccgttgtt tccgcctgca ccgaaatcaa cgccaaagtc cgcctcggcg
     3361 cactggccgg cgtattgggt atggcgggca cgggcaatat tcagggcgaa gaccaaaaaa
     3421 aactggatgt tatcgccaac aacatcatga ttgacacact caaagccaac cctgccgttg
     3481 ccggtttggc aagcgaggaa gaagactctt tcgtaagtgc cggtgaaaac ggacgctatc
     3541 tcgtcctatt cgatccttta gacggatcgt ccaatattga tgtcaacatt tccgtcggta
     3601 cgattttctc catccttgaa aaacccgaag gcgcattggc aaccgaatca ttccttcaaa
     3661 cgggcagaca gcagcttgcc gctggttacg ttctttacgg gcctcaaacc cagctcgtat
     3721 tcacattcgg acacggcgta tatatgttca cgctcaatgc cgaaaacgaa tttgtgctga
     3781 caaaagaaaa accgaaagta cccgaaagta ccaaagaatt tgccatcaat atgtccaacc
     3841 gccgccactg gctgcccccc gtccaacaat acatcgacga gctcttggcg ggcgaaaccg
     3901 gcacgcgcgg caaaaactac aatatgcgct gggtagccag catggttgcc gaaatccacc
     3961 gcatactgat gcgcgggggc gtgttcatgt atccgcaaga caaacgcgac ccgtccaaac
     4021 ccggcaaact gcgcctgatg tatgaagcca atcctatggc cctgatactc gaacaagccg
     4081 gcgcatcggc aagcaacgcc cgtcaggaca tactcggcat ccgtcccgaa agcctgcacc
     4141 agcgcgtcgc cgtcattatg ggcagcagcg aagaagtgga ttacctcaac cgcctgcatt
     4201 caaaataaaa ggcagcctga ccgataaaga aaggcggcag cgcgcatccg acgctgccgc
     4261 cgatatttcg tttatagcct taaaaagata gaaaaccacc tttccgaacg gcaaaaatgc
     4321 cgtctgaaaa gttttcagac ggcattttaa aaattaaagc ccgaatttat ttgcccgctg
     4381 ctttttgcag gatggcgtac aactcgtctt tcagtttcaa tttggctttt ttcagctcat
     4441 cgatggtttc cgcaccgctg gtaaccggat tgttgaccag accggtaatt ttatcgtcca
     4501 gctcgttgtg ttcgtcgaac agacgggcga agcgagaatt ttcctgtttc aatttggaaa
     4561 tcaaatcacg gtattctggg aacatatcgc tttcctctct tggtttacgg ttaaaaagtc
     4621 aaattgaatc tttacattaa ttataacaaa gataatattt ttatccagtg ttttatcgga
     4681 tacggcaaaa ttttctttta acaccgccgt ccggttgata aaaacgttga caatatcacg
     4741 ctttacgcat aaattttcag cctttccta
//
LOCUS       DAWKQB010000150         4733 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.151, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000150 DAWKQB010000000
VERSION     DAWKQB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.151"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(391..825)
                     /locus_tag="WJL14_001815"
     CDS             complement(391..825)
                     /locus_tag="WJL14_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ7656856.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJL14_001816"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WJL14_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ7656857.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2398)
                     /locus_tag="WJL14_001817"
     CDS             complement(1556..2398)
                     /locus_tag="WJL14_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ7656858.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPVPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPGNASAKADSEQGNGE
                     DKGTGIKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2653..3291
                     /locus_tag="WJL14_001818"
     CDS             2653..3291
                     /locus_tag="WJL14_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ7656859.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3743..4543)
                     /locus_tag="WJL14_001819"
     CDS             complement(3743..4543)
                     /locus_tag="WJL14_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ7656860.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
BASE COUNT         1182 a         1214 c         1036 g         1301 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgcctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacaacccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aacactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgggaaaa accactgctt ttcacgattt
     1681 taacggactc aacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 catcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt gataccggtt cccttatctt
     1921 ccccattgcc ctgctcgctg tctgctttgg cagaagcatt gccgggatgt tcggcaggtt
     1981 tttcagacgg cttctcgacc ggtttttccg ccggtttcgg gacaggcttc gcttccggct
     2041 taggctctgg tttcggtttt tcttcgggtt tcggcttttc ttcaggtttc ggctcttcct
     2101 taggctgctg aatatccgca tccgcctttt tcgtaaccac cggcttcaaa accggcttgg
     2161 gcggctcgac aggtttgggc ggctcgggca cgggttgcgg ttcgggcgcg gcaggcgcgc
     2221 ctgcaccttc gggtgcgccg tcccctccgc caaaatcgcc caaatcgaca aattcaataa
     2281 cattgcctga ctctatcacg ggcagcttgt gcgcctgcca gagcaatgcc accattgcca
     2341 aatgcagcag tgcgacggaa aacacgactg cgggggttaa aattcgttct ttatccataa
     2401 ttcgggcata ataatagcaa caattcctat ttgcaaccta tttttacaat ttttggtcat
     2461 atgaatgtct gttccgttca caggcaaacc gtgtttaaac gctgtattac agcaaatcat
     2521 cagataacgg gccggcagaa aaaatgattc cgtctgattt cttattccaa taaaatcagg
     2581 ttagatgata tattgccgct tctgtctgtc agccgtttcg ggctgcacac cacatctgtt
     2641 caaaggaaaa ccatgtttca aaattttgat ttgggcgtgt ttctgcttgc cgtcctgccc
     2701 gtgctgctcg ccattaccgt cagggaggtg gcgcgcggct atacggcgcg ctactgggga
     2761 gacaacactg ccgaacaata cggcaggctg acactgaacc ccctgcccca tatcgatttg
     2821 gtcggcacaa tcatcgtacc gctgcttact ttgatgttta cgcccttcct gttcggctgg
     2881 gcgcgtccga ttcctatcga ttcgcgcaac ttccgcaacc cgcgccttgc ctggcgttgc
     2941 gttgccgcgt ccggcccgct gtcgaatctg gcgatggctg ttctgtgggg cgtggttttg
     3001 gtgctgactc cgtatgtcgg tggggcgtat cagatgccgt tggctcaaat ggcaaactac
     3061 ggtattctga tcaatgcgat tctgttcgcg ctcaacatca tccccatcct gccttgggac
     3121 ggcggcattt tcatcgacac cttcctgtcg gcgaaatatt cgcaagcgtt ccgcaaaatc
     3181 gaaccttatg ggacgtggat tatcctgctg cttatgctga ccggggtttt gggtgcgttt
     3241 attgcaccga ttgtgcggct ggtgattgcg tttgtgcaga tgttcgtctg actggctttc
     3301 agacggcata aacgctccag aaaacgcggc aggacatatt gccctgccgc gttttcctgt
     3361 aatgtaatct tatttttttc atcattatta gaaccaagtt gcatgataat acctttcatt
     3421 aactgaaaca ctgattaaga aactccagtc tgtctaatga tgaggttttc acatcgccaa
     3481 aacttgccaa tcaaatgctg gatttattgc cgtctgagat ttggtcaaat ccaaaggcga
     3541 cattcttaga ccctgtgtgt aaatcagggg tatttttgcg tgaaatcgtc aaacgcttgg
     3601 atgaaggctt gaccaatcaa ataccagata aacaaactcg cattaaccac attttaaaaa
     3661 atcaagtttt tggaagtact gccacgtatg taggtagctt tgaccgatat ttgcataaaa
     3721 actcctttgc tggtgaaagg aattattttg ccaattttaa aatatttctg gcaccaaata
     3781 gtacaatgac aaagacaatc atgccaatga ttaaatcagg atagctagaa tgagtcaata
     3841 acgtcaatgc tcccgccgct atcacaccga tattgatgat aatgtcattg gatgtaaaaa
     3901 tcatgctggc tttgatatgg atttctttat tttgattttt gctcagtaga tataagcaca
     3961 gccagtttgc aatcaatgcc aaaaatgccg tgccaatcat cagttgataa ttgggcagct
     4021 gctcagcacc gataaaacgc ctaatcactt ctatcacccc aaataacgcc aatattatct
     4081 gcgttatccc cgccaaaaat gccacacgtt ttttatacgc cagcgtcata ccaatggctg
     4141 atagcgccaa tatatagaca aagctgtccg ccagcatatc tagactatca gcaatcagcc
     4201 ccatagaatt agcaaaaata ccaaccgaac actctatgat aaaaaacaca aagttaatca
     4261 tgagcacttg atataataat cttttttcta agtgctcatc aggcttgtta aacactatct
     4321 tatcaacaat cacttcggtg gaaatgatat gactatcaaa attaagcggt tcaagtactt
     4381 gtaaaatcgt tgtatcttga ttatcgtgat agacggttaa gcaccgccca gcaatatcaa
     4441 actgtaattc ataaatatca gacacatctt ttaaacgcat gcgaatgagc tgttcttcgg
     4501 acgggcagtc cattttggta atgttaaaaa tggtcttttt catctattta gttccttgtt
     4561 ttgatcaggt tggctcaaat aaatctgtgt ttatattgct gcttggtaat ttttggatgg
     4621 tttgagtaaa ttgattaggt taaaatttac ctttggaagt accgccacgc ataatagttt
     4681 agatatgttt ataatctctg gataaaaaaa cgtaataagt gtttgctgga taa
//
LOCUS       DAWKQB010000151         4727 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.152, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000151 DAWKQB010000000
VERSION     DAWKQB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.152"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            166..945
                     /gene="pgeF"
                     /locus_tag="WJL14_001820"
     CDS             166..945
                     /gene="pgeF"
                     /locus_tag="WJL14_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ7656861.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALDGTPDADAS
                     VDDTGKAVCAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAATAFEGIGSGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            997..1476
                     /locus_tag="WJL14_001821"
     CDS             997..1476
                     /locus_tag="WJL14_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656862.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1478..2476
                     /locus_tag="WJL14_001822"
     CDS             1478..2476
                     /locus_tag="WJL14_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ7656863.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2597..3016
                     /locus_tag="WJL14_001823"
     CDS             2597..3016
                     /locus_tag="WJL14_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656864.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3091..3606
                     /locus_tag="WJL14_001824"
     CDS             3091..3606
                     /locus_tag="WJL14_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ7656865.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3690..4574)
                     /locus_tag="WJL14_001825"
     CDS             complement(3690..4574)
                     /locus_tag="WJL14_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ7656866.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIDEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1054 a         1245 c         1323 g         1105 t
ORIGIN      
        1 ataagggcat cattcccgca ccggcaagaa tccgaacccg aacgtttgaa aacaatcccg
       61 aatctccgaa ttcccgcctg tgtgggaatg acgaaaaaac aagcattcat ttgccccgaa
      121 ggcagttaat caaccctttc cgccacacac ctattccaat atccaatgaa aaccatcaca
      181 gaaaccctaa atctcgcccc gaaaggcaaa aacttcctga ccgccgattg gcccgcgccc
      241 gccaatgtga aaaccctgat taccacgcgc aacggcggcg tgagccaagg tgcgtatcag
      301 agtttgaacc tcggtacgca cgtcggcgac aatcccgaag ccgtgcgccg caaccgcgaa
      361 atcgtgcaac agcaggtcgg attgcccgtt gcctacctca atcaaatcca cagtaccgtc
      421 gtcgtcaatg ctgccgaagc attggacggc acgcccgatg ccgacgcttc cgtggacgat
      481 acgggtaaag ccgtctgtgc cgtgatgacc gcagactgtc tgcctgtttt gttttgcgac
      541 agggcgggta cggcggttgc cgccgcacac gcgggctggc gcggtttggc gggcggcgta
      601 ctgcaaaaca ccatagccgc aatgaaggtt ccgcccgtcg aaatgatggc gtatctcggc
      661 cccgccatca gtgcggatgc gtttgaagtc ggacaggatg tgtttgatgc gttctgcacg
      721 cccatgcccg aagccgccac cgcatttgaa ggcataggca gcggcaaatt ccttgccgac
      781 ctttacgcgc tcgcccgcct gattctgaag cgcgaaggcg tgggcggcgt atatggcggc
      841 acgcattgta cggttttgga acgggatact ttcttttcct accgccgcga cggagcgaca
      901 gggcgtatgg cgagcctgat ttggctggac ggcaatgccg tctgaacacg ccgctgatat
      961 aatctaccga ctttgtgctt ttgagaaagg caagccatga acaaactgtt tcttactgcc
     1021 gcagtgctga tgctgggcgc gtgcggtttc cacctgaaag gtgcagacgg catttctccg
     1081 ccgctgacct accggagctg gcacatcgaa ggcggacagg cattgcagtt tcctttggaa
     1141 accgcgctgt atcaggcttc gggtagggtg gacgatgctg ccggcgcgca gatgaccctg
     1201 cgtatagaca gcgtttccca aaacaaggaa acctacaccg ttacccgtgc ggcagtcatc
     1261 aacgaatatc ttttgatatt gacggttgaa gcgcaggtat tgaaacgcgg cgagccggtc
     1321 ggcaaaccga tgaccgtgtc cgtccgccgc gtccttgctt atgccgacaa cgagatcttg
     1381 ggcaaacagg aagaggaagc ggcattgtgg gcggaaatgc ggcaggatgc cgccgaacag
     1441 attgtccgcc gcctgacctt tctgaaggcg gaatgacgtg gcggcacata tcggacgcat
     1501 tgatacggac gcgcctttga aacctctgta cgtcatccac ggcgaggaag aactgttgcg
     1561 tatcgaggca ttggacgcat tgagggcggc ggcgaagaaa caaggttacc ttaatcggga
     1621 agtttatacg gcagacaatg ccttcgattg gaacgaactg ttgcaaaccg caggcagtgc
     1681 gggtctgttt gccgatttga agctgttgga actgcatatc cccaacggaa aacccggcaa
     1741 aaccggcggc gaggcgttgc aggattttgc cgcccgattg ccggaagata cggtaacgct
     1801 ggttttgctg cccaaactgg agaaaaccca gctccagtcc aaatggtttg ccgcattggc
     1861 ggcaaagggg gaagtgtggg aagccaaacc ggtcggcgcg gcggctttgc cccaatggat
     1921 acgcggacgg ctggacaaaa tcggtttggg tatcgaggca gacgcattgg cactgtttgc
     1981 tgagcgcgtg gaaggcaatc tgttggcggc gcgtcaggaa atcgacaagc tcgggctgct
     2041 gtatccgaaa gggcataccg tcaatatcga tgaggcgcaa accgccgttg ccaacgtcgc
     2101 ccgcttcgac gcgttccaac tggcaggcgc gtggatgaag ggcgatgtcc tgcgcgtatg
     2161 caggcttttg gacggattgc gggaagaggg cgaagaaccg gtgctgttgc tgtgggcggt
     2221 tgccgaagac gtgcggacgc tgatccggct tgctgccgcc ctgaagcagg ggcagagcat
     2281 ccaatccgtc cgcaacagcc tcaggctttg gggcgacaag cagacgctcg caccgcttgc
     2341 ggtcaagcgg atttccgtcg tccgcctgct tgacgcgctc aaaacctgcg cccaaatcga
     2401 ccgaatcatc aaaggcgcgg aagagggcga cgcatggacg gtattcaaac ggcttgtcgt
     2461 gtcgctggcg gaataaagcg gtaatcccca aaatccgaaa atactgtaaa ataccgttaa
     2521 tcctgaaaag tattcaccaa tccgtccgaa aacatttcag acggcacgac cacctcaata
     2581 aaggaacatc aaccctatgg acaataagac caaactgcgc ttgggcggcc tgattttact
     2641 gaccaccgcc gttttaagcc tcattatcgt attgattgtc gattcctggc cgcttgccat
     2701 cctgcttgcc gccgtcatcg tcgccgccgc tgcgggcggc tttgtttgga catcccgccg
     2761 acagcaacgc cagtttatcg aacgtctgaa aaaattcgac atcgatcccg aaaaaggcag
     2821 aatcaacgag gcaaacctgc gccgtatgta ccacagcggc ggacaacacc aaaaagatgc
     2881 gattaccctg atctgcctgt cgcaaaaatg ttcggtggac gaggcgcacg ctatgttcaa
     2941 aaaacgcccg acacgtcagg aaatcaatca aatggcggca aaacagtcgc gcggtcagaa
     3001 acgtccgcac cgttaaccgt ccgaggcata tctgtatgaa tgccgtctga agcctgttgg
     3061 cgtttcagac ggcatattct gattgaaaag atgatgacac tgaaaaccgc cccgctcaaa
     3121 cgccgctttg ccgccatgct gtacgaaatg ctgctggtcg gtgcggcaac ctgtttggca
     3181 gcattgtttg ccggtattgc cgccattttt ctgaatcccg tttctatcgc ggtttctgca
     3241 ttggtaacga gtatcctgat aatgggatca tggtggcttt attttcgcgc caactggcac
     3301 ggtcaggggc agaccttggc gatgaggaca tggaaaatcg gcttgtgcga ccttaacggc
     3361 atacagccgt ctttgcacct gctgcgcctg cgctttattt gggcgtgcat atttatcgtc
     3421 ttcattccta tgcttgctta tgccggatta cgccacttcc tcggcattcc gcccaagggc
     3481 gcggccggcg cggcattgat ttggctgatt ttaccgtggg ggttcgcact gctgaatccc
     3541 gatcggcagt ttctgtatga ttttcttgca ggaacaagat tggtggcggt caaaggaaag
     3601 ccttaagcct ttataccgca aaggtttcaa actgaaaaaa tgccgtctga aagggctttc
     3661 agacggcatt tggttatcgg ggaaaccgat tattcgatat tctgcacttg ttcccgcatc
     3721 tgctcgatta agactttcag ttcgaccgag gcttgggtgc attcggcggc aatggatttg
     3781 ctgcccaaag tgttggcttc gcggtttaat tcctgcatca ggaaatccaa gcgttttccg
     3841 ctgctgcctt tgtgttcggt aacgatgcgg cgtacttcgt cgatatgcgt gcgcaggcgg
     3901 ctgaattctt cgtcgatgtc ggatttttga ataaagaggg caaattcctg ttgcagcctg
     3961 tcattattga tgtcgccgac tgcttcgacg aggcgggcgc ggattttttc tttatgtgtt
     4021 tccagcaggg taggaaagag ttcgctcaat gcatctatga tttcttccat gctttcgagg
     4081 cgttgtaaca ggtgctcgcc caattttttc ccttcgcgcc ggcgtgcggc agtaaagtct
     4141 tttaacgctt tttcggtcag ttcggtaatg ctttttgcca attcctccgt attttccctt
     4201 tggcttgcca atacgccggg gaaacgcagg atgtcggcaa cgcccagttt tgccaaatcg
     4261 tgatgcttgc ggaggttttt gttgatttcg gcaagctggt tcaccaaatc cgagttgagt
     4321 tccaaggact gtctgctgtt ttccgtatct tgaatttgga ttttgcattc gactttgccg
     4381 cgcgcgatat gggatgaaat tttctcgcgg ataccgcttt ccaaatagcg caaatcatcc
     4441 ggcatcctga tttgaatgtc caaaaagcgg tggttgacgg cgcggatgtc gaggttgatg
     4501 cgtttgctgc cgcactctgc cgccgcgttg gcaaagccgg tcatgctgtg gatgtggata
     4561 tttccgctgc tcatgtcgtt ctccgaagcc cgttaaaatg gaatcaatat atcacatctg
     4621 tatggcggca agcgttttcg ggtgtgaaaa attgaaggtt ttgcagcggc agattggaat
     4681 cacgctcttt tgttgctgca aggaagggaa atgtatagtg gattaac
//
LOCUS       DAWKQB010000152         4530 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.153, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000152 DAWKQB010000000
VERSION     DAWKQB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.153"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(190..1053)
                     /gene="rpoH"
                     /locus_tag="WJL14_001826"
     CDS             complement(190..1053)
                     /gene="rpoH"
                     /locus_tag="WJL14_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ7656867.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(1317..2855)
                     /gene="lnt"
                     /locus_tag="WJL14_001827"
     CDS             complement(1317..2855)
                     /gene="lnt"
                     /locus_tag="WJL14_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ7656868.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPFGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(2924..3292)
                     /locus_tag="WJL14_001828"
     CDS             complement(2924..3292)
                     /locus_tag="WJL14_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ7656869.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            complement(3192..3650)
                     /locus_tag="WJL14_001829"
                     /pseudo
     CDS             complement(3192..3650)
                     /locus_tag="WJL14_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            3846..4127
                     /locus_tag="WJL14_001830"
     CDS             3846..4127
                     /locus_tag="WJL14_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656870.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSARLGMPLLLVSPANVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYISWLGLAEPCRFRR"
     gene            4127..4519
                     /locus_tag="WJL14_001831"
     CDS             4127..4519
                     /locus_tag="WJL14_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HEZ7656871.1"
                     /translation="MMPPVTGVPNSHLLFDVAFAVVVLSLLIRGMTIPVMARLLKVAM
                     PNKPEPKDTHDIWLAKKEIVRMSAFKVVAESEAEGHHPDTVEPISDSFGARCFALIRN
                     GIRIEMQSDTVLQAGIWRGTSCPTARSI"
BASE COUNT          976 a         1038 c         1411 g         1105 t
ORIGIN      
        1 attccgccaa tctgtcggaa aagcagctga tgcggcagtg tctggtgcat gtctgctttt
       61 tgatttcggc aattgcaacg gcgtggacgg ataaaatcgt gtacagcacg atacacaagc
      121 ctcattgggg tttctattat aaaaaaacac ccgacaaacc ggcttgtcgg gtgtttaatt
      181 atgtctattt caaaccgctt cggcttcttc ggtcaggaaa ccacgcagtt tctgcatggc
      241 ttttgcttca atctggcgga tgcgctcggc agagacgccg tattctgcag caagctggtg
      301 cagcgtcagc ccgccgtcgt cttgaagcca gcggctttcc acaatacggc ggctcctgtc
      361 atccagttgc gccaaagcgt tttgtaaacc ttctgtttgc agggcgtaat gcgcctgttt
      421 cgatagttgt cggctcggtt cggaatcgtg gtcggcaagc cagtcgatgg gggcgaaact
      481 gtcctcgtcg tcgctgttgt ctgccatgat ggcgatgtcg tgtcccgtca tgcgctgttc
      541 catttccaga acttcggaaa gtttgacacc caaatcgtcg gcgatgtctt gtgcctcttt
      601 gggagacagg gcgttgaggt ttttacgcat actgcgtaaa ttgaaaaaca gcttgcgttg
      661 cggtttggtg gtggcaacgc gaaccaaacg ccagtttctc aaaataaact cgtggatttc
      721 ggctttaatc cagtgtacgg caaatgaaaa cagacgcgcg cctcgacctg gctcgtagcg
      781 tttgaccgcc ttcatcagtc cgatattgcc ttcctgaatc aggtctgcct gattcagccc
      841 gtagccgtca tagccgcgcg cgatggaaac gacgacgcgc aagtgggaca ggatgagttg
      901 tttggcggcg ttgaggtcgc cctttatccg gcgttcggcg aggcgggttt cttcttcctg
      961 ggacagcatc gggatgctgt tgacggtgtg gatgtattgt tcgaggctgc cgttgccgct
     1021 ttggatggcg ggtaatgcga aagcgttatt catttggggc atttcctttc ggctgaaact
     1081 gcgtatcggc ggtttgctgt gttgggatgc agtatatcac tgcttggctt gtattttgta
     1141 tgtttggcag gagatatgcg ctaaggtttg aaagacagga aaattttgta aggcaggttt
     1201 gattgatttt gtaaacctga tggctcaatc cgatttcgga attatattac atacgtagtt
     1261 gtatgtaaac agccggtttg aaaaaagaca gcccgtctgg acgggctgtg caggtatcag
     1321 tgttctttgt ttcggaagat gaaaagaatc agtgcggcta gggtcaatat gcccatcaac
     1381 caccatgaac tgccggtttt catatagggc gtttcgccga catagccttt gatgtgtcct
     1441 tccaatacgg tttccgtatc gggttgggct tgggcgatga tgttgccttt gggggagatg
     1501 atggcggttg cgccggtgtt ggtggcacgg accatatagc gtccgagttc catagcccgc
     1561 gcctgcgatt gttggaggtg ctggtacatg gcgttggatt ttccgtacca cgccatattg
     1621 ctggcattgg caagcagtgt ggcatctttg gcggcggcaa tcagttcgtc gccgaatccg
     1681 tcttcgtaac agatgttgaa ggcgattttt tggtttttca tcagcagggc ggattgctta
     1741 ccgccgcctt tgcggaagtc ggaaaggggc atattcatca ttttgtaaag cggcgtggtc
     1801 aggaagggca gcggtttgta ttcgccgaag gggacgaggt ggtttttggc gtagtagggg
     1861 atgccgtcct gattgttttc ctgataaccg gtcaggttga tgacggcgtt ttcgtaaccg
     1921 ttgccgtccg aagtgtattg gctgatgccg acggcgagcg cgctgccgtt gttttgcgcc
     1981 tgttcggcaa attttgccag tatgttttcc ggcaggtttt ggcgcatgac ggggatggcg
     2041 gtttcgggca ggatgacgat gtcggcagtg gttttgccga cctgttcgta atatttctgt
     2101 atggtcggga tgacttggtc ttcacgccat ttgagggttt ggtcgatgtt gccttgaagc
     2161 agggcgacgg tgctgcggct gccgtcgggg cgggtgaagt cggtttgtcg ggcggtgtag
     2221 cctgcggcaa gcagggcggc aatcaggata attggaagca ggcgtttgcc cgaacgtgcg
     2281 gtgttatcac tcgccaaaac cagccagaca ccgagaaagg cggttgccag tgtaaccatg
     2341 tggatgccgc caaatggggc aaagccggcg agcgggctgt ccggggtgat ttgggagtag
     2401 ccgattgcgc cccagccgaa tccggtcagg aaacgttcgc gggcaaactc ggtcagtgtc
     2461 cacaggatgg gcagtaccaa accgattttt atgccccgag gcagggtaaa ttttttccac
     2521 agccagaaac acagtgccgg ataaagggca aggtaggcgg ggagtaggaa ggtcagcggt
     2581 acggcataga ggtcgggcag gccggaaacg tcgtgcaggg cggtgtgtat ccagtagaac
     2641 tgtgtcgtgt atgcggtcag gccgaacagg taggcggaag agacagcaaa acgcggacgc
     2701 agttcgatga ggcggacgaa ggcaccgaaa atcaagggca tcagccaaaa gtggtagtag
     2761 ggtgcgaagg taaagggggt ggcggcggca aaaaggatga gcaaaggcca gtagagggcg
     2821 gggtgctgcc agtatttgtc cagtttggaa accatattca tctgtctgtt cggaagatac
     2881 cgtctgaaca tctttcaaac ggcatcggta tttgaaaaag aaatcaatgc ctgccgaaac
     2941 gattcatcag cggcaaggcg ggggcgcagg caatcgaacc ggaaagcagc ccgacaacga
     3001 ggttggcgaa gtactccgcc cagccagcgt cccagtgttg cgcgtgcagg aaatcgtgca
     3061 gaaggccgag gttgtgggcg accagtacgc cgccgataag aaacatggca agcgtgccga
     3121 ccacgctcaa accgcgcata aagcaaggca taaaggcagt cagcatttgc cccaaactgc
     3181 gcgaaaaggt ttgtgggcgg cgcatcagca gcatgcctaa gtcgtcgagt ttgacgatga
     3241 cggcgacgat tccgtacacc aaaacagtca tgccgatgcc gattgccgcc atcacgagcg
     3301 agcgcgtcat gtcggtgcag aaacttgtgc agcagctttt ccacgccttc aaagcacaga
     3361 taaatcccgc ctgccgtcaa aagcggcgta atgagttgcg gcaggaaggt ggaaagcagc
     3421 agagccgcag gtaccaaaac cggcttgttg gaaaaagaac ctttcgccat cgaccaaaca
     3481 atcggcaact cgcgttctgc cgatacgccc gtaacccggt tggcattggg tgccaaatcg
     3541 tcgccgacca tgccggcggt tttctttgcg gcggctttgg tcatcagggc aacatcgtcc
     3601 aaaacggcgg tgatgtcgtc cggcagggta aatagtgagg caaatgccat taaagaatcc
     3661 tgaaatgcgg cgcaaagtcc gacattatat aggagaacgc ggatttgggc ggtttcaggc
     3721 ggcatgaaac aggaaaatgc cgtctgaacg ctgtggcgga cgtgaagtaa agtttcgtga
     3781 aaagaaaatg ccgtgttaca gtctttcgat tttaatttaa tgaattttaa gggagaatcg
     3841 ttagcgtgga ttggatgggc agtctgttcc tgccgggtgg cgcactgttg tttctgagcg
     3901 tggtttcgac cactttgtcc gcacgtttgg gaatgccttt gctgctggtt tctcctgcca
     3961 acgtgttgga cggggcggcg gaagccttgg cgattgcggc gttcctgatg ctggtcgcgc
     4021 gtccgccggc agtgttcggc ggtttgtgga aattcaatta cagcctgcgt gaaaaggcgt
     4081 atatcagttg gttgggtttg gcggagccgt gccgatttcg ccggtgatga tgccgccggt
     4141 aacgggcgtt cccaattcgc acctgctgtt tgatgtggcg tttgccgtgg tggtgctgtc
     4201 gctgctgatt cggggaatga cgattcctgt gatggcgcgg ctgttgaagg ttgccatgcc
     4261 caacaagccc gaaccgaaag atacgcatga tatttggctg gcgaaaaagg aaattgtcag
     4321 aatgtcggcg tttaaagtgg ttgccgaatc tgaagcggag ggacaccatc ccgatacggt
     4381 cgaaccgatt tccgattcgt ttggcgcacg ctgtttcgca ttgatacgca acggcatccg
     4441 aatagaaatg cagtccgaca ccgtgcttca ggcggggatt tggcgtggta catcctgccc
     4501 gacggcaagg tcgatatagt ggattaaatt
//
LOCUS       DAWKQB010000153         4498 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.154, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000153 DAWKQB010000000
VERSION     DAWKQB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.154"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(57..1850)
                     /locus_tag="WJL14_001832"
     CDS             complement(57..1850)
                     /locus_tag="WJL14_001832"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ7656872.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFGSAKPKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="WJL14_001833"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="WJL14_001833"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ7656873.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKLRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="WJL14_001834"
     CDS             3218..4480
                     /locus_tag="WJL14_001834"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ7656874.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRNRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1025 a         1235 c         1254 g          984 t
ORIGIN      
        1 ccgcaatgcc gtctgaagcc tttttatgct tcagacggca tcgtctgttt tcacgctcaa
       61 atcgtaccgc tgccttgcgc tttcggtttg gcagaaccaa attccagttt gcttaaggca
      121 gagaggtagg ctttggcagt ggcgaccaaa acgtcggtat ccgcaccctg accgttgaca
      181 acgcggttgc cacgcgccag acggacgctg gtttcgccct ggctttccgt accttgcgtg
      241 acggcgttga cggaataaat ctgcaaagcc gcgccgcttt gcgccacgct ttcaatcgct
      301 ttgaaaatcg catccacggg gccggagcct gtggcggaag cgcgtttttc ttcgcctttg
      361 atgctgaaca cgatgtcggc gcgcggctct tcgccggttt cggtgctgat tttttgggag
      421 atgaatttgt agctctcggc attcatgctg cccatttcgt cggataccag tgcgtgcagg
      481 tcttcatcga agatttcgcg ttttttgtcg gcgagttctt tgaagcgggc aaacgcggcg
      541 ttcagtgcct cttcgctttc caactcgatg cccaaatccg ccagcttggt tttgaaggcg
      601 ttgcggccgg acaatttgcc caagctcaaa cggtttgttg cccagccgac cgattcggcg
      661 gacataatct cgtaagtttc gcggtgtttc agcaccccgt cctgatggat gcccgattca
      721 tgcgaaaagg cattggcacc gacaatggct ttgttgggct gcacgggata gcccgtaatg
      781 gtggacacca gtttggacga aggcacgatt tgcgtggtat cgatgccggt ttccaagccg
      841 aacaagtcgt ggcgcacttt caacgccatc acgatttctt caaccgaagc attgcctgca
      901 cgttcgccca agccgttgac agtacattcc acctgacgcg caccgccttt taatgcggca
      961 agcgaattgg caaccgccaa gcccaaatcg ttgtggcagt gtgccgacca aacgactttg
     1021 ccgccgttgg gcgttttgac aatcagttcg cggaaaaatt cttcggtttt gtacgggatt
     1081 gaatagccga cggtatcggg aatattgatg gtggtcgcgc ccgcttcaat taccgcgccg
     1141 cagatttcgg caaggaaatc gatttccgaa cgcaacgcgt cttcgcagga aaattccaca
     1201 tcgtcggtgt attcacgagc gattttcacc gctttgaccg ccgcctcaat cacctgcttc
     1261 ggcttcatct tcaatttgta ctccatatgg atggggctgg tggcgatgaa ggtgtggatg
     1321 cgttttttcg gcgcgggcgc aacggcctca cccgcctgac ggatgtcccg ctcgatggca
     1381 cgggacaatg aacagaccgt tgatttggta atggttttcg caatcgcatt gaccgcctcg
     1441 aaatcgcccg ggctggcagc ggcaaaaccc gcttcgatga tgtccacacc caatttttcc
     1501 agctggcggg cgacgcggat tttttcctct ttggtcatag cggcgccggg cgattgttcg
     1561 ccgtcgcgca gggtggtgtc gaaaatgata acgcggttgg cctgtgtcat ttctgttctc
     1621 tccagagatt cgtttttttg taaaaaagca ttcaaatcca gcggcctgcc ttgcgattcg
     1681 gcaagcgcgg tcagcttttg cagttgcgcc aaaggcagcg acccgcgcgt gcgccattta
     1741 tagatggcgg cggtcgtcgc ggcattttcg ggatcgtgct gtttcaacgc ttcggcaagc
     1801 gcgttcacgc cgccgaaata agcaaccaag cggtctatat ctaattgcat ggtagtcctt
     1861 gtctaaaata aatcagccta cagcagaaat atggacagga ttatacgcat tttggacaaa
     1921 acggcaatat aaatttgaaa aatctcaata agccgccata gattttatta attttataca
     1981 ttttgtctat tttatcgcag acgcggcgtg attgttgaca attgcggcag aacagggcaa
     2041 attcggcggc acaaggcggc aaacgagaca ggacaaacca acaaggacgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacccg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcac aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacc ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggctcgggt tcggcaaacg gcacaggcgg tatgctgact aaaatcaaag cggcgacgat
     2761 tgcgaccgag tccggcgtac cggtctatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagcggca gataatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagcagg ggcggcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg aaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac cgtaaccgtg tacagcaagg caaccaaaca
     3061 gcctttgggc aaagggcgag tcctgttcgg ctctgccgcc gccgaagacc tgctcaaatt
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgttt gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtcaagac ttggaagccg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacggc aaacgcattt gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactttgccc aacggcttgg aaatcgtcaa
     3541 aaagcgcgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gagaatgccg tcgtgccggt cattgaaacg gggacgggca ttgtccacat
     3901 ttatatcgac aaggacgcgg attgggacaa agcgctccgt atcgtgtaca acgccaaaac
     3961 cagccgcccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacggctgtt ggttcgcaac cgtatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gccgtgaaaa ccgtcgcttc
     4201 ggtcgaagag gcggttgggc acatcgaagc ccacggcacg caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg cccgatgggt ttgaaagaac tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctg
//
LOCUS       DAWKQB010000154         4431 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.155, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000154 DAWKQB010000000
VERSION     DAWKQB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.155"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(1..134)
                     /locus_tag="WJL14_001835"
                     /pseudo
     CDS             complement(1..134)
                     /locus_tag="WJL14_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1142..1912)
                     /gene="xth"
                     /locus_tag="WJL14_001836"
     CDS             complement(1142..1912)
                     /gene="xth"
                     /locus_tag="WJL14_001836"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ7656875.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1972..2193)
                     /locus_tag="WJL14_001837"
     CDS             complement(1972..2193)
                     /locus_tag="WJL14_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ7656876.1"
                     /translation="MLIQLLDGDCKITELSESLSMPATAVSNHLSRLRVEGLVDFTRY
                     HRIIEYRLISEDAATILRTIRDLENKRAV"
     gene            complement(2504..3385)
                     /gene="nadC"
                     /locus_tag="WJL14_001838"
     CDS             complement(2504..3385)
                     /gene="nadC"
                     /locus_tag="WJL14_001838"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ7656877.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQVVRAGQTLAAVEGNACAL
                     LAAERTALNYLTHLSGIATATARAVAEVAKYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(3460..4398)
                     /locus_tag="WJL14_001839"
     CDS             complement(3460..4398)
                     /locus_tag="WJL14_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656878.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
BASE COUNT         1023 a         1111 c         1191 g         1106 t
ORIGIN      
        1 gccacttggg ctttgccgtc aaaatgcgtg tgggtgtggt cgtcgatttg tttgtccaat
       61 tcgtcaatca gccggtcaaa atgggcaatc agttgtttga cgcttccgac ttgcgtttca
      121 tgaaactgat gcagatatag tggattaaca aaaatcagga caaggcgacg aagccgcaga
      181 cagtacaaat agtacggcaa cgctgtactg gtttaaattt aatccactat attttgtaat
      241 agctgccatt atgcctacta ggatatatac cttctatata atccttattt aaataaaaac
      301 ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa gattttatct ttctcatcag
      361 gtttatattt gctatcaata tcaaaaagag aataaggatt acaatgagtt cgacatacta
      421 ggtaaaaatt tatatcttgg atcatgaaat attcgatatt tatcaggatc gaaagatgct
      481 aaaaattcat caatattggt actggatatt ttgtcatcac taataaacat cgctagagat
      541 gggcgacttt gattttccat ttgttgatta gtaaaggata taaatactaa cttgccattg
      601 tcaaaccaca tagatttata tagtggatta aatttaaacc agtacgtcgt tgcctcgcct
      661 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
      721 tatagttatt gcctttggga gaatcaattc tgacagcaca taacataggt tcatctttac
      781 agtcgttata ttgggtttca tcaaactcaa ctgcacagca caataaagac aataggaaaa
      841 aaaggagatt aatttattac tatacataag gcagcctgat tattcattct aaaataaatc
      901 cggttttcaa tatttgattt tattccggat tatatacaca ataaatttta ccgctccaaa
      961 atctgatatt aaccctatgg atttcataaa ttcttcagaa aaataggctg tgcttgatca
     1021 atccgccgac tccggctgtt tgtcagggaa aggcaattat ttttcggtca ggcagaaagt
     1081 tatcctatcg aatcacttga agatatgaat gcttgtcaac gccatatcaa aacacggtct
     1141 tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct cggacgctcc agcgcgcgcg
     1201 tctccaaatc gacacggaca tccttcaacg ccgccgccat cgcaggcgac accaaaatat
     1261 ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc gcgatagtcg aaccaggtat
     1321 agaacgcgcc ttcgggatgg acttggcgca ggctgtcggt cagtcccaaa tccagcaggt
     1381 tttgaaacca ctgccgttcg acggacgaac agtggatttt ttcgtgccat ttttcagggt
     1441 cgtaacagtc cgcatcggca ggcgcgatat tgaaatcacc cagcagcacc agtttgccgt
     1501 agcgggtcat ttcatcgcgg acaaactccg tcagtgcggc aaaccattgt tctttatatt
     1561 tgaatttcgg gctgtcgagg gcttcgccgt tgacgcaata gacattgatg acgcgcacac
     1621 cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc cggcagcgcg ggcagcccga
     1681 tatgcacgtc ttccggcgcg ttgcggctga cgattgccac gccgttgtag gttttctgcc
     1741 cgctccaaac acagtgccag cccatcattt gcaaagcggc ggccggaaat ttgtcctgat
     1801 cgagtttgag ttcctgcaaa accaaaatat cgggcggatt gtcggcaagc aggttttgca
     1861 cctgcggcag ccgcacattg agcgaattga cgttccaagt ggtgattttc ataacatttc
     1921 cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa ggattttaac actataccgc
     1981 gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt gccgcgtctt cggaaatcag
     2041 gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc agaccttcca cgcgcagtcg
     2101 gctcaaatgg ttggaaaccg ctgtcgcagg catggacagg gattcggaca gctcggtaat
     2161 cttgcaatcg ccgtccaaca gttggatcag tatagtggat taacaaaaat caggacaagg
     2221 cgacgaagcc gcagacagta cagatagtac ggaaccaatt cacttggtgc ttcagcacct
     2281 tagagaatcg ttctctttaa gctaaggcga ggcaacgccg tactggtttt tgttaatcca
     2341 ctatacctgc ctgaaacgct gttttttcta aagcatcacg accttcacga aaaatgccgt
     2401 ctgaagaata tttcagacgg catttcctcc ttgaggcaat aaccggatta cggttaggcg
     2461 gttccttgtc tgacatatca gacaactgcc gacaccctaa aactcatgcc aagaaatcca
     2521 aacctatgtc caacgagcgg ctgctgtggg tcagatagcc gagggcgatg ccgtccacgc
     2581 cggtttgcgc cacgcgcttc aggcggtcga agccgatgcc gcccgatgct tcgcaataga
     2641 cggtgtgggg gtgggcggtt tgcgtgtggc agcggtttgc cgcttctttc agggtttcgt
     2701 cgtccatgtt gtccagcaaa atccgttccg cgcccgctgc gatggcttcg tccagttgtg
     2761 ccaacgtgtc cacttcgatt tccacgcagg tcaatgctcc gaccgcctgt tttgcctgcc
     2821 gaacggcttg ggcgatgctg ccgcaatagg cgaggtggtt gtctttgatg agcacggcgt
     2881 cgtccaagcc catgcggtgg ttcacgccgc cgcctgccct gacggcgtat ttttgcagga
     2941 cgcgcagcag ggggatggtt ttgcggctgc acacgatgtc tgtaccgtat ttggcgactt
     3001 cggcaacggc acgcgcggtg gcggtggcga tgccgcttaa gtgcgtgagg tagttgagcg
     3061 cggtgcgttc ggcggcgagc agcgcgcagg cgttgccttc gacggcggca agcgtctgac
     3121 ctgcgcggac gacttgcccg tctcggattt cggcttggaa gcggacggac ggatccatcg
     3181 tctgaaaggc gaggcgcgcc aagtccatac cggcgatgac gccgtcttcg cggctgacga
     3241 ggaagagttt ggcggtttta tcgggcgcga tgacggcggc ggacgtaata tcgccgcgcc
     3301 tgcccaaatc ttcgctcaag gcttgttcca ccatggggcg caacagggtg tcgggcaggg
     3361 ggaagagggt gttttcagac ggcatggggt ttcctttgtt gaaaaagatt gatgttgcac
     3421 cgatgggcaa aacttcgttt gatgtcgtct gaaaacgggc taacggctgc ccagcggcaa
     3481 tccgatgtcc gaaatcagcc tgtcgggcag gacgtcgtcg cggaagcggc aaagctgcgc
     3541 gggacggcct ttgctgcccg atacgccggt atccgacggc tcgataaggt tttgctgctg
     3601 aatctggcgg cggaagtttt gcttgtgcag caatctgccg ctgatggctt cgacgctgtt
     3661 ttgcagttgc agcaacgtga attcgggcgg catcagttca aaaatcacgg ggcggtattt
     3721 gattttggcg cgcaggcggg acagggcggt cgccagtacg cgacggtgat cgtggcgcat
     3781 gggctgcccc gtgagcgcga agtcgaagtt tgcctgcggc tcggcggctt ccactatcag
     3841 accgctttca tacagcattt catagcgttg taaaacgtat tcttccgacc agttttccgg
     3901 ttcgaccccc cagcacaaat gaatgcgctt gagccgcatt tgacgcactt cctccgtgtc
     3961 cgccgaattt gcccaaatgc gcaggcggct gacgacggcg tcgcgctgcc cgccgtcggt
     4021 gcgcaagtct tcccacggga aatagccgta gcagtcctgc catttcgcat ccgggtgcag
     4081 gatgctgtcg gctgcctcgc gcaccagccc caaatagctg acgtacaaga cgggcatgcc
     4141 gtgttcgttg cggcggtggg tatcgacaaa ggtgtaaagc tgttccacat agcccatagg
     4201 ctgcgaagtc tgcttggcaa cccacagctt cacgcccgcc tgcagggaat tgcgcagagg
     4261 ggagagcggg ccgttgggca ggagtgtgcc ttgggcgacg gttaataccc gcaggccgcc
     4321 gtcggtaacg gcaatcaata cgggaaccag ctcgacgatg ctttgcggcg gggcttcggc
     4381 ttcggggtag gcatccatgc tcggctttct gcgggtggga aaggctttat t
//
LOCUS       DAWKQB010000155         4396 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.156, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000155 DAWKQB010000000
VERSION     DAWKQB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.156"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(44..3088)
                     /locus_tag="WJL14_001840"
     CDS             complement(44..3088)
                     /locus_tag="WJL14_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ7656879.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAGFEQVQRSRIAETDHLADDVLN
                     GGWQEETAAIANDGSEGVAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSRRASDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPGATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETILGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDNPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGMSDDLLGISRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3253..3933
                     /locus_tag="WJL14_001841"
     CDS             3253..3933
                     /locus_tag="WJL14_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ7656880.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     DSAGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3962..4321
                     /locus_tag="WJL14_001842"
     CDS             3962..4321
                     /locus_tag="WJL14_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ7656881.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPASLSPATGNLFANWTGA
                     FLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGTAVK"
BASE COUNT          900 a         1289 c         1111 g         1096 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcgattat agccgatacg caatgcgcgc tgcacgccag
      181 aaatgctggc tttgcgcgtt ttcaaaacaa cggacacggc ctcgtcgtac atcggatcgg
      241 tttcgccgtc gccgctccgg ctgattccca gcaaatcgtc ggacataccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gtttcagata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaacgcg ccgtgaacgc gctgcggata ggccgtaccc ggcggcagga
      421 acagcatatc gccctgcccg agcaggtttt ccgcacccat ttggtcaaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacactg gggcgttgtg tggcaaggat aagatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cttcgatttt cttgcctgcc gtcatcatca
      661 ggtcggcaaa ctcatcaacc acgaccacga taaacggcaa tttttccaaa ggttcgggat
      721 tgtcgggcgt gaggctgaac gggttgccga ttttctcccc ccttgctgcg gcttcggcga
      781 ttttttgatt gaaacccgca agattgcgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggtgctttgc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggcgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccgag gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag atttagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgccccgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcggat accgcaccgg tttcttcaga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatgcccgg cgggacgggc gttcagacgg cacattttca aacggacaaa
     1621 ccgcctgact gtcatgcccg aacgcttcgg tttccgacaa atattgcccg cttgaccgct
     1681 ctgccacacc ctcactgccg tcattcgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tgatcggttt cggcaatgcg gctgcgttgc acctgctcga
     1801 atcctgccgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacagtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgcccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctttcccctg ccctgcatga cggcggaacg gcgcattccg tgcattttcg gctttgactg
     2281 cagaaaaggc agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact
     2341 cggaaaacgc ttctttgtcc gcatcgaaca tacgggaata cgtgcgttga agcacagggt
     2401 cgtccaaacc gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga
     2461 ccgctgtttc gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg
     2521 cgctgtttaa agcacgcgtt gcttcttcca aagtgatttc cttaaaaacc ggacggacgg
     2581 gcgaaatttc gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc
     2641 ggcggttgtc gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag
     2701 cttctgcttc ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg
     2761 atgcggtcgg aacatccgca cgatttgcag aagcaggcgg aacgggcctg tccggttcgg
     2821 attcgtgcgc cagatagttt cggcagaaac ggacgacacc gacaaacagc caatacactg
     2881 ccgttttgac ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt
     2941 tgatttcagg cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc
     3001 gcatccactc gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca
     3061 aaataacgat caaaactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg
     3121 ggtttatttt aacgtgtttc catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac
     3181 cgattcggct ttcggtttgc ccccgccgac agtataatag ccgtcccttc ccttaatctt
     3241 tgcggcattg ccatgatttt ccaaacagtc tggttttcag atatggtact gtcggtttcg
     3301 tggattgtcc ttatcctgat actggcagct tccgcgccgt ccgcattccg ctcgcttgcc
     3361 cgctaccgtt ccgcccttcc actgtgtacc gtaatttttt ccgcagcatg gtgtctgaac
     3421 gcctctgccg gcggcggaca gcttgcacag atgaactacc acctgctcgc cgtcaatctg
     3481 gttgcactga tggtgggcac ttccgccgcc ctttggcttg ccgcgctgct gatgctgccc
     3541 tactgcctgc tgtttgccga ttctgccggc gcatatccgc cgaacgcttt ggtgctgatt
     3601 ctgcccgcgc tggtcgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccgcccaat
     3661 attttcatct ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgttgacc
     3721 gggctggtgc tgaccggtat tttggatgct gcaaacgctt ttccgtccga aatattgtgg
     3781 acgaccgccc tgcccgtctt tattctgctg gcgtgggcgg aagccttcct cagcggcatt
     3841 tcaactgcca tatttgttgc actgaagccc cattggatta acactttcga cgacaaccgc
     3901 tatcttaagt ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac
     3961 catgctttcc aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg
     4021 gctgctcaac ctcgccgttc ccgcatcgtt gtctcccgcc acgggcaacc tgtttgccaa
     4081 ctggacgggc gcttttctta tcggaatctt tgccgaaacc gtcaaccatc cgcaatggaa
     4141 gctgctgctg attaccggtt ttctcggcag cctgaccacg ctttccggat tttcactgga
     4201 aaccgtaacc ctgctccaat cgaatcgtcc tgcttcagca cttgccaata ttttcctgca
     4261 cacggcaggt tcgctgctgc tgacttggct cgggctgaag atagggacgg cagtcaaata
     4321 accgcaacaa attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta
     4381 aatactggct gtgcca
//
LOCUS       DAWKQB010000156         4301 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.157, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000156 DAWKQB010000000
VERSION     DAWKQB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.157"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            38..461
                     /locus_tag="WJL14_001843"
                     /pseudo
     CDS             38..461
                     /locus_tag="WJL14_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WJL14_001844"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WJL14_001844"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ7656882.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NILMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WJL14_001845"
     tRNA            1210..1285
                     /locus_tag="WJL14_001845"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WJL14_001846"
     tRNA            1311..1386
                     /locus_tag="WJL14_001846"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WJL14_001847"
     tRNA            1410..1485
                     /locus_tag="WJL14_001847"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WJL14_001848"
     tRNA            1507..1582
                     /locus_tag="WJL14_001848"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="WJL14_001849"
     tRNA            1608..1681
                     /locus_tag="WJL14_001849"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="WJL14_001850"
     tRNA            1784..1873
                     /locus_tag="WJL14_001850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="WJL14_001851"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="WJL14_001851"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ7656883.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3946)
                     /locus_tag="WJL14_001852"
     CDS             complement(3080..3946)
                     /locus_tag="WJL14_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ7656884.1"
                     /translation="MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGWAINAV
                     IAGKVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLNNSLECDNHFIRKGDRRQLYRHYRLVARLRVLISNREAFGYLCVG
                     AAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSERNIKAGT"
     gene            complement(3957..4076)
                     /locus_tag="WJL14_001853"
     CDS             complement(3957..4076)
                     /locus_tag="WJL14_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ7656885.1"
                     /translation="MQRSHKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
     gene            complement(4192..>4301)
                     /locus_tag="WJL14_001854"
     CDS             complement(4192..>4301)
                     /locus_tag="WJL14_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7656886.1"
                     /translation="VEQSFGTLHCKFRYARAAYFGLICARCRLKGSPDA"
BASE COUNT         1088 a         1040 c         1106 g         1067 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttcag cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccccatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc gccgccgccg ttatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcctgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatca tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 ttaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaaca tattgatgtt tatcgcatct ttgctgacgg
     1081 tttggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgt gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgcg agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac catggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatac
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aataacaata ccgctccaag cggttatttt tttatctgtc
     1921 ggacggggat ggacaatatt gatatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagaaga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatctgcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctca
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggcg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgcttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgttccgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gcgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca accagtctgt
     3361 aatggcggta cagctgccgc cggtctcctt ttcggataaa gtggttgtcg cattccaagc
     3421 tgttgttcag gcggaaatac aggttttcgc taatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gccgctgccg ctgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca cattaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac accttccccg caattacggc attaattgcc cagccgccaa
     3841 acaccggata caccagcatc aaaaggtttt ccagtccgac cagggaaaat gtgccaatca
     3901 atcgcttgcg gtgggtttgg gctatgtgtt tcaacatttt ccacatattt gcccccctat
     3961 tttttatttt ccgagattgt tttttccatc acttcagcca gtgcatccaa atcggcaaac
     4021 agtcgggttg tgcgcttgtc gccgaatgtg gcaaatactt tgtgagacct ttgcaaaatt
     4081 cctttccctc ccgacagccg aaacccaaac acaggttttc agctgttttc gccccaaata
     4141 ccgcctaatt ttacccaaat acccccttaa tcctcccctg atacccgata atcaggcatc
     4201 cgggctgcct tttaggcggc agcgggcgca aatcagtccg aaataggctg cccgagcgta
     4261 gcggaattta cagtgcagcg taccgaagct ttgttcaacc a
//
LOCUS       DAWKQB010000157         4115 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.158, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000157 DAWKQB010000000
VERSION     DAWKQB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.158"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..144
                     /locus_tag="WJL14_001855"
     CDS             <1..144
                     /locus_tag="WJL14_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEZ7656887.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IQT"
     gene            274..1710
                     /locus_tag="WJL14_001856"
     CDS             274..1710
                     /locus_tag="WJL14_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ7656888.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSIAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTHQPRSPVRPALAVSLILLSIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQFNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWLITFHFVCLTFVVFNTANPD
                     DAGAVFSALFANANGWNAPQQADMLLLASFASAMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1721..2704
                     /locus_tag="WJL14_001857"
     CDS             1721..2704
                     /locus_tag="WJL14_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ7656889.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAAPETAPQTGET
                     EWKQNTEAAAVRTGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHYVQVVWLGIPYMKKAKLDGQMRYLDKLLSEYLKGKIILIPTAHTLSGGKDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2701..3894
                     /locus_tag="WJL14_001858"
     CDS             2701..3894
                     /locus_tag="WJL14_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ7656890.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNHGWAAKDGVHFSAKGYQRSAEMLADSLEELVRS
                     AAIRQ"
BASE COUNT          902 a         1337 c         1034 g          842 t
ORIGIN      
        1 aacgtcggta tcggcagcgg cgcactgttg ggacattggg taacgcaata tgtcggtatt
       61 tcccgtatcg gcatcgccgg tatgcttgcg gcggcggccg ggctgtggct gtgcctgaac
      121 ttgaaccgcc atatccaaac ctgaccgcgc gatgccgtct gaagcccctc ccgcccttca
      181 gacggcattt tgattgaaga aatatccgcc cctgcttaaa ataacgggct ttgccgtgtt
      241 ttaacgccta ttttcttatt ccaaggatgg tttatgccgc tgctgtctgt cgagttcgca
      301 ctgttctttc tcgccttcct gccgatttac tggggcttgg cgaaataccc gtccgtccaa
      361 aacctgctgc ttttggctgc cggtatgggc tggctctacc atatcggccc cgtatttgcg
      421 gcaatcgtcg tcctttattc ctcctgcgtg taccttttgg gcgaactgct ccgttccgat
      481 cgcgaaaata cgcgccgctt ttggctgggg tgcggcattg ccgtctcgct gaccgtcttg
      541 ggctttttca aatatttcga ctttttccgc ccgctgattg cacaatatgc cggacaaggc
      601 ggcgcaatcg acatcctgat gccgctgggg ctttcgtatt acaccttcca gtcaatcgcc
      661 tatttggttt actgcttccg tgcgccgcac gccgcgcgtt tcgagtggca cgaactgctg
      721 ctgcacctga gttttttccc caccgttacc tccggcccga ttatccgcgc cgccgcattc
      781 aaaagcgcgg acggcgaaca ggcgggcgca ttggcgcaaa tccgtaccca ccaaccacgc
      841 tcacccgtcc gccccgcact cgccgtttcc ctgattctgc tgagtattgc caaaaaatgg
      901 tggctggcgg ggatgctggc ggaaaactgg gtatcgcccg tatttgaaaa ccccgcccaa
      961 ttcgacggct ggggcgtatt ggcgggcgtg tacggctata ccttccaact cttcctagac
     1021 ttttccggat attccgattt ggttatcggc atggcaatgc tgctgggctt taggctgcct
     1081 aaaaacttct ccgcaccgct tcgtgcttta aacatccgcg cattttggga caaatggcac
     1141 atcagccttt ccacctggat acgcgactac atctacatcc ccttgggcgg cagcaaaaaa
     1201 ggctttttgc ggacacagtt caacctgatg gcggcgatgg tactctcagg catctggcac
     1261 ggctacggct ggaatttcct catttggggc gcgctgcacg gcacggcact ggtgctgctc
     1321 aacacgggcg accgctattt cggacgcaac gcactatgcc gtctgaaata cctcgcaccg
     1381 ctctcgtggc tcatcacctt ccattttgtc tgcctgacct tcgtcgtctt caataccgca
     1441 aaccccgacg atgcaggcgc agttttcagt gccctctttg ctaatgccaa cggctggaat
     1501 gcgccgcaac aggcagacat gctgctgctt gcctcgtttg catccgccat gctgctctac
     1561 ccttacctgc aacgcgcttt cgacggcgcg gtcaaaggtt tggaaaaaat cccgatgtgg
     1621 ctgtggttta tccccgtttc cgccgtcctg ctgctgatta tcgtattggc cccctcgggg
     1681 atacccggct ttatttatgc caatttttaa gggtttggac atgaaaaact ttctttccct
     1741 tttcgcctcc atactgatgt ctgccctgat tgccgtgtgg ttcagccaaa accccatcaa
     1801 cgcctactgg cagcagacct accaccgcaa cagcccgctc gaaccgcttg ccgcctacgg
     1861 atggtggcgg agcggtgcgg cattgcaaga aaacgcctac gccctttcag acggcatcaa
     1921 agccttcctg tccggcgaaa cgccgccgac ggctcaagac ggcggttcgg cagatatgcc
     1981 gtctgaagcc gccgcacccg aaaccgcccc tcaaactggc gaaacagaat ggaaacaaaa
     2041 caccgaagcc gccgccgtcc gaacagggga caaagtcttt ttcgccggcg actcgctgat
     2101 gcagggcgtt gcacccttcg tgcaaaaaag cctgaaacag caatacggca tcgaatccgt
     2161 caacctcagc aaacaaagca cggggctgtc ctacccctca ttcttcgact ggccgaaaac
     2221 gattgaagaa accctgaaaa aacatcccga aatcagcgtg ctcgccgtct tcctcggtcc
     2281 gaacgacccg tgggatttcc ccgttggcaa acgctacctc aaattcgctt ccgacgaatg
     2341 ggcgcaagaa tacctgaaac gcgtcgaccg catccttgaa gccgcacaca cgcactacgt
     2401 ccaagtcgtc tggctcggca tcccctacat gaaaaaagcc aagctcgacg gacagatgcg
     2461 ctacctagac aaactgcttt cggaatattt gaaaggcaaa atcatcctga ttcccaccgc
     2521 gcacaccctg agcggcggga aagaccgcta caccgactcc gtcaacgtca acggcaaacc
     2581 cgtccgctac cgcagcaagg acggcataca ctttaccgcc gaaggacaaa aactgctggc
     2641 ggcaaaaata atggaaaaaa tcgtttttga accaagtacg caaccatcaa gtacacagcc
     2701 atgaacccca aacacctcat cgcattttcc gccctattcg ccgccacgca ggcagaagcc
     2761 ctacctgtcg cctcagtcag cctcgacacc gttaccgttt ccccgtccgc cccctacacc
     2821 gatacaaacg ggctgctgac cgactacggc aacgcctccg cctcgccttg gatgaaaaaa
     2881 ctccaatccg tcgcacaagg cagcggcgag accttccgta tcctgcaaat cggcgactcg
     2941 cataccgccg gcgacttctt taccgacagc ctgcgcaaac gcctacaaaa aacttggggc
     3001 gacggcggca taggctgggt ttaccccgcc aacgtcaaag ggcagcgcat ggcggccgtc
     3061 cggcacaacg gtaactggca aagcctcacc agcaggaaca acaccggaga cttcccgctc
     3121 ggcggcatcc tcgcccacac cggcagcggc ggcagcatga ccctgaccgc atcggacggc
     3181 atagcaagca agcagcgcgt ttccctgttt gccaaacccc tgcttgccga acaaaccctg
     3241 accgtcaacg gcaacaccgt ctccgccaac ggcggcggct ggcaggtact ggatacgggc
     3301 gcggcactgc ccctgaccat acacaccgaa atgccgtggg acatcggctt catcaacatc
     3361 gaaaatcccg ccggcggcat taccgtttcc gcgatgggca tcaacggcgc acaattaacc
     3421 cagtggtcga aatggcgtgc cgaccgtatg aacgaccttg cccaaaccgg cgccgatcta
     3481 gtcatccttg cctacggtac caacgaagcc ttcggcgaca acatcgacat tgccgatacc
     3541 gaacagaaat ggctggatac cgtccgccaa atccgcgaca gcctacctgc cgccggcatc
     3601 ctcatcatcg gcgcgcccga atccctgaaa aacacgctcg gcgtatgcgg cacacgcccc
     3661 gtccgcctga ccgaagtcca acagatgcag cggcgcatcg cccgtcaggg gcagacgatg
     3721 ttctggtctt ggcaaaacgc gatgggcggc gtttgcagca tgaaaaactg gctcaaccac
     3781 ggatgggccg ccaaagacgg cgtacacttt tccgccaaag gctaccaacg gtcggcggaa
     3841 atgctcgccg acagcctcga agaactcgtc cgctccgctg caatcaggca ataatcggac
     3901 aggaggcgga cggtatttcc gcaacagggg gatgccgtct gaaacgcata ccttcatatt
     3961 gcttcagacg gcatagccac cccgcgcacg gtttgccgga cgcaaccggc attcgcctca
     4021 agcatcggaa ggacgggggc gaacctccgg catacggcgc aaaggcggcg tttgatatgc
     4081 cgtctgaagg caaagatgat aaactgccgc cttcc
//
LOCUS       DAWKQB010000158         4017 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.159, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000158 DAWKQB010000000
VERSION     DAWKQB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.159"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            414..2294
                     /locus_tag="WJL14_001859"
     CDS             414..2294
                     /locus_tag="WJL14_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ7656891.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQKGWQ
                     KGLHAVLFADYEFGLPLTGVDSERGGNLAMHWFADCADIDAASWLAQHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENRRCALLDRHLCRLKTAAQALN
                     LPLPDGCENQIKQYIAHLPDGAFRIKALLASDGISLSRAVLNHLADKQRVIISPTILP
                     AQNYLRRFKTTHRALFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
     gene            complement(2353..3870)
                     /locus_tag="WJL14_001860"
     CDS             complement(2353..3870)
                     /locus_tag="WJL14_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ7656892.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVDNDAPRVVDGDFLLAHPKMLEHSLRDVLNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          961 a         1325 c          909 g          822 t
ORIGIN      
        1 aaggcgaggc aacgctgtac tggtttttgt taatccacta tacttggagc tgatcttgct
       61 tttcgcctaa ttctacgttt tcaaacggtt gcagctggtg gtctgccata aaggtctcct
      121 tattgtattt caggttggaa atgtttcaac acacaggacg acacataaag cgtcgcccta
      181 tgtgttgccc tgatttggaa ggggttacgc ccctcccaaa taaagtctga ttctaccgcc
      241 ctaaagggcg gggtttaaac cgaaaaggaa atacgatgaa aatccgtttt cacaatttta
      301 caccctcgcc ccccactttc tctacataaa attacatttt gccgatattt gccgaatcgt
      361 ctgaaaatat gtgtaaaaag agcgtataat caaaacattt gccccgaatt gccatgccct
      421 atttcgccct gtttgacgat gccgtaagcg gccgcgcaaa acgctatcaa aatcatgtag
      481 aaagccgttt tttccgtccc gaagaactcg atgctttgga cggcgcgctg caaaagggct
      541 ggcaaaaagg gctgcatgcc gtgttgtttg cagactacga attcggtttg ccgctgacgg
      601 gggttgattc cgaacgcggc ggcaatcttg ccatgcactg gtttgccgac tgcgccgaca
      661 tcgatgccgc aagctggctt gcccaacatt cagacggcat ccccgccggc atttccacgc
      721 cgcaatcctc cgtatccgaa accgattacc tcgaccacat ccgccaaatc cacgaagcca
      781 tccggcgcgg cgacacctat caaatcaact acaccacccg cctgcacctg caagcctacg
      841 gcaatcccgt cagcctctac cgccgcctgc gccagcccgt cccctatgcc gtcttgtccc
      901 acctgcccga tgtggagggg caatccgcgt ggacgctgtg tttctcgccc gaactcttcc
      961 tcaacatcgc ttcagacggc accatcagca ccgaaccgat gaaaggcact gcgccgattt
     1021 tgggcgacgg acaagacgaa cgccgcgccg ccgagttgca aaccgacccg aaaaaccgcg
     1081 ccgaaaacgt gatgattgtc gatttgctgc gcaacgacct cggcaaaatc gcccaaaccg
     1141 gcaaagtatg cgtgcccgag ccgtttaaag tatcgcgttt cggcagcgtc tggcagatga
     1201 ccagcaccat ccaagcccaa gccctgccaa acacctcgtt cgccgacatc ctccgcgccg
     1261 ccttcccctg cggcagcatc accggtgcgc ccaaaaaaat gagtatgcag attatcgaat
     1321 cgctcgaaac cgaagcgcgt gggctttata cgggcagcat cggctacctg aaaccttgcg
     1381 cgggcgggtt ggggtttgaa ggcatattca acgtcgtcat ccgcaccttg tcgctcaaac
     1441 ccgtttcggc ttcagacggc attgtttcag gcatcggcgg cccggattca aacgcgcaag
     1501 cccgaacggc agggcaaggc ggggcaacgc cgcatccgtt tgactccaat ccgccgtatc
     1561 ggggcgtgta cggtgtcggt tccggcatcg tcatcgacag cgaccccgcc gccgaatatc
     1621 gcgaatgcgg ctggaaagcc cgtttcctca acgaattgcg ccccgatttc ggcatttttg
     1681 aaaccctgcg cgtggaaaac aggcgatgcg ccctgctcga ccgccaccta tgccgtctga
     1741 aaaccgccgc ccaagccctc aacctgcccc tgcccgacgg ctgcgaaaat caaatcaaac
     1801 aatacatcgc ccacttgccc gacggcgcgt tccgcatcaa agccctgctc gcttcagacg
     1861 gcatcagcct gtcccgcgcc gttttaaacc atttggctga caaacagcgc gtcatcattt
     1921 cgccgaccat cctgcccgca caaaactacc tgcgccgctt caaaaccacc caccgcgccc
     1981 tcttcgacca agcgtggcaa accgccgaaa cacaaggcgc gttcgacagc ctgtttttca
     2041 attcagacgg catcctgctc gaaggcggca gaagcaacgt gttcgtcaaa catcgcggac
     2101 aatggctcac gccctcttta gatttagaca ttttaaacgg cataatgcgc caagccgtgt
     2161 tggacgaacc gcaaaaatat ttgcaaacaa atcaagtaat cgaaacacac atcacacaaa
     2221 aaacactgca agaagccgaa gaaatccgcc tctccaacgc cttgcgcggc gtatttgccg
     2281 ccgcccttgc ctgaacgcgc aaaaatgccg tccgaacctg tttccaaagt tcggacggca
     2341 ttatcccacc attcaaaacc gccaatccgc cgacacaaac acctcgctgt tgcggcgttt
     2401 cgcatacggc acattacttt ccgtcctgcc gaaacgataa ttcaacgcgg gcacgatacc
     2461 tttgtacgac aacttgtcgt ggctcaaagc cagcgagaca ttccattcgc ggttgcgttg
     2521 cgcctctgtc gagaaagccg caacgccctt atagttgcgg cgggcataag acgcggaaac
     2581 ccgactgttc aaaccgccca actgccgcca ctcctgcgcc cagccggcat aaacaccgtt
     2641 gcgccggtag gcggcattat tgaccgcgcc gcccaccgtt tcgcgtttcg gcacaaaccg
     2701 cacaaactgc cagccgccga acacagttgc cgattcgccc aaacgttttg ccgacgaaac
     2761 ataaaacccg tcctgccgac cgttattgta ttccgcccta tcctgttcgc ggtagcgttg
     2821 gcggtaatgt tccagcgcga ccgaaaattg ccatcccggg tttgggcggt aagtatggga
     2881 caactgcacg ccgactccgt gcgccagcat atacggcggc aggcggcggt tgtttacccg
     2941 ttttgttttc gcatcaaagc cgtcgctgcc cgacaactgc acctgataaa acggcaaaat
     3001 ccccgccgtc tgccgtgcat ttttatactg ccaacccaaa tacgctctgc cgaacccgtc
     3061 gtcataagct gattttttac tgaaataata gctcgtgccg ccgatattgg aacggaacaa
     3121 caaataatga ttatctgcca acgccgtcag tttttccgcc tcgatttcat aattcaagcc
     3181 tgccgcccgc tccgcccggc tgacactgca tatctgccgg cctccgtttt gccggcaata
     3241 ctgcggcgcg gcattattgg catttctatt gaccgccgga ctgatgccgc ccgaaaaacg
     3301 ccagcccgtc agcccctccg cttttttccg aaaacgcccc acattttcca aaaccggcgc
     3361 cggcaaatcc aatttttccg cctcggcaaa atgcctttct gccgacttca gccggaaatc
     3421 gtcaaactcc gccgccgcca aatccagcaa aatccgctcg tctgccgcat tttccccgtg
     3481 cagttcccga taccgcgcca ccgcctccgc cggccttccc gccaatttcg ccagcaaagc
     3541 ccgcgccctg ccgtacaaaa ccgcgtcata atccggcagc ttggcataca aatccgccaa
     3601 cgaagcgatc aaatccgcct gattgccgtt gagcacgtcg cgcaaactat gttccaacat
     3661 tttcggatgc gccaacaaaa aatccccgtc aaccacgcgc ggggcatcat tgtcaacttt
     3721 ccaatccgat tccgcccact tatccgacac cgaccgctgc acctgcaaca atgccttgtc
     3781 atccaaaatc gcgggcgcat ccgccccata ggcggcagaa acacctgccg cacaccaaac
     3841 aaccaaaaaa ccgtatctga aatacaacat accctgtcat ttacctttct ggcaaacacg
     3901 ccgccgaagc acgtcaaacc atccgaaaaa caggcagaaa cccgtgaaaa ccggctttac
     3961 cgtctgaaag cgggcaggca aaaaaccgcc gcccgatttt caaagggcgg atttcac
//
LOCUS       DAWKQB010000159         3996 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.160, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000159 DAWKQB010000000
VERSION     DAWKQB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.160"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(67..1317)
                     /locus_tag="WJL14_001861"
     CDS             complement(67..1317)
                     /locus_tag="WJL14_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ7656893.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1384..1579)
                     /locus_tag="WJL14_001862"
                     /pseudo
     CDS             complement(1384..1579)
                     /locus_tag="WJL14_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WJL14_001863"
     CDS             complement(1798..3573)
                     /gene="ggt"
                     /locus_tag="WJL14_001863"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ7656894.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3741..3975)
                     /locus_tag="WJL14_001864"
                     /pseudo
     CDS             complement(3741..3975)
                     /locus_tag="WJL14_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
BASE COUNT         1001 a          956 c          938 g         1101 t
ORIGIN      
        1 tccgcctgaa acattatgag atttcaggcg gcattggatt gcttagcgga atatttttaa
       61 aaaggcttac gcgccgtaaa cggggtattt attgcacaaa gcagttactt gttcgcggac
      121 tttggcgagg ttggcttcgt cttcggggtt agacaatacg tcggcaacca agttcgccaa
      181 tacgcgcgcg tcggcttcgt taaaaccgcg tgtggtcatg gcggcggaac cgatgcggat
      241 gccggaggta acgaagggtt tttccggatc attcgggatg gcgtttttgt tgacagtgat
      301 gtgcgctttg cccaaagcag cttcggcggc tttgccggta attttcatcg gttgcaggtc
      361 aacgaggaaa acgtggcttt cggtgcggcc ggaaacgatg cgcaaaccgc gtttaaccaa
      421 ctcttccgcc atggcggctg cattgatttt cacttgtttt gcgtattgtt tgaactcggg
      481 ttgcaatgct tctttaaacg ccacggcttt ggcggcgata acgtgcatca gcgggccacc
      541 ttgcaggctt gggaagatgg aagagttcaa cgctttttcg tgggtattgt cgcggcacaa
      601 aattacgccg ccgcgaggac cgcgcagggt tttgtgggtg gtggtggtaa cgaagtcgca
      661 gaacggcacg ggattgggat attcgccgcc ggcaaccaga ccggcatagt gcgccatatc
      721 gacaaagagg tatgcgccga ctttatcggc gatttcgcgg aattttgccc agtcgatttg
      781 caacgcgtag gcagacgcac ccgccacaat cattttgggt ttgtgttcga gcgcgaggcg
      841 ttcgacttcg gcataatcga gcacttcgtt ttcatccaaa ccataagtaa cagcgttgta
      901 gagtttgcct gagatattaa cgctcgcgcc gtgggtcagg tggccgccgt gcgccagaga
      961 catacccaaa atggtgtcgc ctggttttaa aacggaagcg tacacggctt ggttggcttg
     1021 cgagccggag tgcggttgga cgttggcgta ttgcgcgccg aacagttttt tgacgcggtc
     1081 aatcgccaat tgttcgacaa tatcgacgta ttcgcagccg ccgtagtagc gtttgccggg
     1141 gtagccttcg gcgtatttgt tggtcagctg ggaaccttgc gcttccatta cggcgcagct
     1201 gacgtagttt tcagaggcaa tcagctcgac gtggtcttgc tggcgttggt cttcttgggc
     1261 aatggctgct gccaaatcgg ggtcatattg tgcgagggta acgctttttg aaaacatgtt
     1321 ctcggctcct ttgtgtaatc agggtatcat gagtgttttt tgtataaaaa aatacttcaa
     1381 aacctaaggc agatagtcca taatgcgtaa attttctttg gcattatcag gtaatttatt
     1441 taacatgctg gtttttagcg tctcattacc tttatgttag tacaaggcta cccaaaggca
     1501 atgcatcaaa tggtaaattt tcggcagttt gtgcaaaatc aatcaaataa ccagcaccaa
     1561 ccacatcttt atcagccata acccgctgat acaccacttg ccaagacaac tctcctgccc
     1621 caccatgtct taaaatcaga tgaatggctt gtaatgctgt gtttggcgtc tcacaaattg
     1681 cgaagatctg ctcagaagct tgcttgatgg cttgtatttt tgtttgatta ctcacaattt
     1741 ccccctattt taatattaac ttaatgcggt caaattcaca aaatacaagc ttcacctcta
     1801 atcacccatc actcgacctt ctcggcgtgg atcggcacca ccaaccagcc tgcttggctc
     1861 gataataatg gcttgaacac ctgaatttag ctcacgcacg tcagtcttat agcccaaatc
     1921 atttaatgct tgttgccact ggagggcggt tgtacccgtt tctagttcat agctaccaaa
     1981 gcgatttaat aaattgggtg cactgatggc atcttggata tccatattcc agtcactatg
     2041 tgccacaatc gtcttagcga catagccaat gatacggcta ccacctgggg agccgattgc
     2101 catataaggc ttgcctgctt taaatacgat ggttggtgcc attgaggagc gtggtctctt
     2161 gccgggctcg acacgattgg cgacctgttt gccctgcttt attggctcaa aactaaagtc
     2221 tgtcagctca ttattcagca ggtagccatt tgccatcaaa gttgagccaa acgcattttc
     2281 aatggaagtc gtcattgata gcacattgcc cgccttatcc acaattgata tatgactggt
     2341 agaaggtaac tcaatcgctt gtgaggacac ccactcatga ataaaatcgc ctgcagatac
     2401 gctaggcaat gccttatccg actgctcaag cagctggctg cgatgtttta ggtagtcttt
     2461 agaaatcaac tggcgaatgg gtactggtac aaaatcaggg tcgcccaaat atacatcacg
     2521 atccgcaaac gcaagcctag aagcgtcgcc caagagacgt aaaccttcgg catcataccc
     2581 cacctgattg ggtgaaaatt catttaaaat ccccaaaatc tgacccacag caatccctcc
     2641 tgagcttggt gcacccatac cgcatacttc ataaatacga taagtcacac aaacaggcgg
     2701 gcgttccacc acttgataat cagataaatc ttgtaaggat aattgaccgg ggttatcctt
     2761 agcattttgg acaactgaaa cgatattttg ggcatattta ccagtatgca gagcttttgc
     2821 accttgagct gctaacgcct gaacactgtc agcaaattct aaatttttca gcaagctgcc
     2881 tgcttgtagc ggcacaccat tcggcaaaaa ataagcggct gtttttggat agcgtgccaa
     2941 atgctgctga ttttgctcaa ccgagatggc aagccttggc gacacctcaa agccttgttt
     3001 tgccaagcgg atcggtgtat caaataattt tccccaaggc aatacaccgt atcgctgatg
     3061 tattgtctcc atcagtttag ggatagcagg cgtacccacc gagcgaccac cgaccaccgc
     3121 ttccataaat ttcaatggtt gaccatcttt atccaaaaat aattccggcg tcgcacgcat
     3181 cggtgccgtc tcacgcccat caaatgtggt caatgttttg gcggtattat cccaatacaa
     3241 cacaaatgca ccaccgccca agcctgacga ctgtggctct accaagctta gtgtcgtctg
     3301 caccgccacc atcgcatctg cagcgctacc gccttgcttt aagatatcat agccagcttg
     3361 tgttgctaat ggattggctg acgctaccat aaaatcactt gcaatcacct gcttttgttc
     3421 ggtcagtccc gttgcatgtt caggcgtgtg agcgtctgca cctgtgatga cagcagaatg
     3481 agtattaacc ttaccttgat tggcatggat gacttgacat ccagagattg tcatagacat
     3541 tatcaatgca gtcaataaat atgttttagc cacaagcact ccttcgcctg agtttgattg
     3601 atgattcata caaggcatgc tgattattgt atttaatatg actaaataat tcaatccaaa
     3661 ctatcaatct tgaccatcaa aaaaagaccg ctaatgtcat cagcagtctt ttttgatatt
     3721 tattttaaga tattaagtaa tcagaccttt gggctatgct cttcaatgag tggttttagc
     3781 tcacctgatt ggtacatttg tgataatatc actaccaccg attaactcac cattaaccca
     3841 aagctgtgga aaggttggcc gattggcgat gagtggtaga gtactgcgaa tttctgggtt
     3901 ttctaggata ttaacaaaag caaagggtct gccaattggg tcagcacctc tactgcacgc
     3961 gctgaaaatc cacattgggg aaactggggc gtgcct
//
LOCUS       DAWKQB010000160         3986 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.161, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000160 DAWKQB010000000
VERSION     DAWKQB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.161"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WJL14_001865"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WJL14_001865"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ7656895.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="WJL14_001866"
     CDS             1160..2290
                     /locus_tag="WJL14_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ7656896.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /locus_tag="WJL14_001867"
     CDS             2361..3212
                     /locus_tag="WJL14_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ7656897.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="WJL14_001868"
     CDS             complement(3295..3957)
                     /locus_tag="WJL14_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ7656898.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          876 a         1174 c          976 g          960 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgata tgcgtggctt tcacgccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcgag
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaat catacgccgc tgtccaacag cgtgtattac gcctatttcg aaccttactc
     1501 cgaagaacag catttaaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatcg gcaatcaggt
     1621 cgaaagcgat ctgaaaatct ggattaccgc ccgccagcat ccgggcgaaa ccatggcgga
     1681 atggtttgtc gaggggctgc tcggcaggct gctcgatccg caagacccga cagcgcgcac
     1741 cctgctcgac cgcgccacgt tctatatcgt ccccaatatg aaccccgacg gttcggcact
     1801 gggcaacctt cgtaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tttggaaaaa agccccgaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccgttcaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgctcg
     2521 acgacttcct gacatggggc attttgggcg taattttggg cgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggtc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccactgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagac ctcgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcact cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa tttgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc caatgggttt tgcgcc
//
LOCUS       DAWKQB010000161         3845 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.162, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000161 DAWKQB010000000
VERSION     DAWKQB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.162"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(96..533)
                     /gene="rnhA"
                     /locus_tag="WJL14_001869"
     CDS             complement(96..533)
                     /gene="rnhA"
                     /locus_tag="WJL14_001869"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ7656899.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDALVGRHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            646..1515
                     /gene="tehB"
                     /locus_tag="WJL14_001870"
     CDS             646..1515
                     /gene="tehB"
                     /locus_tag="WJL14_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ7656900.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKVENLPEVLLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSMYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFDVTAADHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWEQVEYKEELGSMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(1565..2371)
                     /gene="thiD"
                     /locus_tag="WJL14_001871"
     CDS             complement(1565..2371)
                     /gene="thiD"
                     /locus_tag="WJL14_001871"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ7656901.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCEAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
     gene            2432..2629
                     /locus_tag="WJL14_001872"
     CDS             2432..2629
                     /locus_tag="WJL14_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656902.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            complement(3673..3748)
                     /locus_tag="WJL14_001873"
     tRNA            complement(3673..3748)
                     /locus_tag="WJL14_001873"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3712..3714),aa:Glu,seq:ttc)
BASE COUNT          853 a          938 c         1117 g          937 t
ORIGIN      
        1 tctggattcc cgcctgcgcg ggaatgacgg tggaggacga tgccgtctga atcccattag
       61 cggtttcaga cggcattttc ttgtcggtgc ggcagtcagg aaaactgcgc tgcgccacgg
      121 tttgccaaat cgtcggcgcg ttcgttttcg gcgtgtcccg cgtgtccttt cacccaagtc
      181 cagctgactt ggtgccgtcc gaccagagcg tcgagttctt tccacaagtc gtcgtttttg
      241 acgggctgtt tggcggcggt tttccagccg ttgcgcttcc agccgtgtat ccagttttcc
      301 ataccgtttt taacgtattg cgaatcggtg cagatgatga cggtgcagcg gcgtttgagc
      361 gatttcagcc cttcgataac ggcggtcagc tccatgcggt tgttggtggt ttgcgcttcg
      421 ccgccgaaaa gttctttttc gtggctgccg tagcgcatta atacgcccca gccgcccgcg
      481 ccgggattgc ctttgcacgc gccgtcggtg taaaggtaaa cgggtgtgtc catattgtgc
      541 ctttgtgtgg tgtccggcgt gattataagc ggtttgcgcg ccgtgccgtc tgaaagattg
      601 cggcggattg aaataggtat ataatgtgca actttatttt tgaatatgaa ggagcggata
      661 gtgggtcaaa gcggcgagtt gttttgtttc ggacagatgc ctgtatggaa agtggaaaac
      721 ctgccggaag ttttgttgtc gggttattcg tccgaaggag gggaatgggt ctgcctgaat
      781 gtgttgcagg gcgatgtcga agtccgcacg caggacgggg cggcagaggt ttggtcggcg
      841 gaaagcggcg attgcgtgtt tgcgccgcag caggtgtttt cggtcaaacc gaaaacggac
      901 gatgccgaaa tccgcttgtc gatgtattgc gcggcggcag actattttca caaaaaatac
      961 gggatgagtg ccacgcattc tgcggtcgcg gcggcacagg ataccgtacc ggcgggcagg
     1021 gcgttggata tgggctgcgg acaggggcgc aacgcgctgt tcctcggttt gaaggggttt
     1081 gacgtaaccg ccgccgatca caatcccgcc gccttggcaa acgtggcgga gctggcagag
     1141 gcggagggct tgaacgtccg cacgctggaa tatgatttga atgccgccgc cttgcagggt
     1201 gagtttgatt atattgtggc aacagtggtg ctgatgttcc tgatgccgca gcgcgtgcct
     1261 gacgtgattg ccgatatgca ggcgcatacg gcggcgggcg ggtacaactt aatcgtatcg
     1321 gcaatggata cggcggattt cccctgcccg atgccgttcc cttttaaatt taaagagggc
     1381 gagctgaagg attattatcg ggattgggag caggtcgaat ataaagaaga attgggttct
     1441 atgcacgcca aagacgaaaa cggcaatccg atacggttta aatttgtaac gatgctggcg
     1501 aaaaagcctg aataatccgc aaaaatgccg tctgaacatt ttgtttcaga cggcattttt
     1561 acgtctagtc ccgatacgcc caatgattga ccgggccgtg tcccgcgccg atttccaaag
     1621 ggtttgagat tgccgccgtg atgtaggcct tggcagtctg tacggcttcg caaacgtccg
     1681 agccttttgc caattcggcg gtaatgcagg cggaaaacgt gcagcccgtg ccgtgcgtgt
     1741 gggcggtcgg aaagcgcggg ctgtccaatt ccagcgtttc gttttgcgta aacagccaat
     1801 ccgtgcagcg tccgctgctg ctgccattca aatgtccgcc tttgatgacg acatttttga
     1861 caccgtaatc aagcaggatt tttgccgcac gttccgcatc tttacggttt tcgatatgca
     1921 cgccggtcag ggcttccgcc tcgggcaggt tgggggtcaa tacatccgta tcgggaagca
     1981 gcaggcgcgt cagtgccgca acggcggaat cctgcaacag cggcgcaccg cctttggcaa
     2041 tcatcacagg gtcgagtacg cgcctgccga agtcgcagtg tttcagcttg tcggcaacgc
     2101 attcgatgat ttccgccgtg ccgagcattc ctattttgta ggcgcggatg tcaaagtctt
     2161 ccctgattgc ttggatttgt gcggtgatgg tttcggtcgg gacgagatga accgccgaca
     2221 cgcccaaggt attttgtgcg gtaacggcgg tgatgacgca cgttccgaac acgccgcgca
     2281 tctgaaacgt tttcaaatcc gcctgaatgc ccgcaccgcc gcccgaatcc gaaccggcga
     2341 tggtaagcgt ttgcacaaaa gagcttttca tttgttcctc cttaatattg tgtccgataa
     2401 aaagggctaa tgcctgtaaa aaagcacggc gatgccgtct gaaaaccgca gcgcgggggt
     2461 ttgtggtgca ttatatgccg tattgcagga caaggcacag ggcagggcgg cggggtgcat
     2521 cgtagcaact gtatttttgc ccgacggggt gaaaaataca gttgctacac ccactgataa
     2581 tgaaaaaaga gagcaatgcc gtctgaaccg cgtgttgcgg cgagcataaa ccggcacgct
     2641 ttattcgtca tttccacaaa aacagaaacc taaaattccg tcattcctac gaaagcggga
     2701 atccagtgtg ttcggtttca gttgtttttg ggtttcgggt gatttccaaa tcatcattcc
     2761 cgcgcaggcg ggaatctagc ccttggggca gcggcaatat ttataaagcc gtctgaaaat
     2821 tcagaagttc tagattcccg ttttcacggg aatgacgaag ggttgtggga atgacgaagt
     2881 ttcagacggc atcgccccgc cccgtcattc ccgcataagc gggaatccaa accattgggc
     2941 agagcagcct taaaggttat aaaagactcg tcattcccac gaaagtggga atccagacct
     3001 gtcggcacgg aaacttatcg gataaaacgg tttcttcaac cctacgctct agattcccgc
     3061 ttttgcggga atgacgaagt ttcagacggc atcaaatgct ttgtcaggct tcagacagca
     3121 tcgtccaccc tctgccgtcc ttcccgcgca ggcgggaatc caatgtgttc ggtttcggtt
     3181 gtttttgggt ttcgggtaat ttccaaatcg tcattcccac gaaagtggga atccagacca
     3241 ttgggcggcg gaaatattca aaagtcgtct gaaaattcag aggttctaga ttcccgtttt
     3301 cacgggaatg acgaagggtt gtgggaatga cgaagtttta gacggcattg aatactttga
     3361 tgctttggtg ggctgaagcc caccctacaa tatagagaaa atgccgccaa acggtatgga
     3421 agcggaaaca atggtggggc tggcttcaga aacaatggta gggtgggctt cagcccaccg
     3481 ctttagacgg catccgcgca ccgtcattcc cgcgcaggcg ggaatccagc cttgtcggca
     3541 cggaaactta tcggataaaa cggtttcttc aaccctacgc tctagattcc cgttttcacg
     3601 ggaatgacga agagtgtgag aatgacggtt cataaataaa aaaacctgct taaaacaagc
     3661 aggtttttga attggcacgc ccacggggat tcgaaccccg gttgccgccg tgaaagggca
     3721 gtgtcctagg cctctagacg atgggcgcgt gggaatgggc attatacata cttctagaaa
     3781 tttagcaata cgatgccgtc tgaaacttcg tcattcccgc gaaagcggga atctagaatc
     3841 tccaa
//
LOCUS       DAWKQB010000162         3838 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.163, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000162 DAWKQB010000000
VERSION     DAWKQB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.163"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..412)
                     /locus_tag="WJL14_001874"
     CDS             complement(<1..412)
                     /locus_tag="WJL14_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ7656903.1"
                     /translation="MTHISSPRNEFIRGIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTSMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNM"
     gene            560..1012
                     /gene="dut"
                     /locus_tag="WJL14_001875"
     CDS             560..1012
                     /gene="dut"
                     /locus_tag="WJL14_001875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ7656904.1"
                     /translation="MNIEVEMKVLDERMADFAPAYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1089..2276
                     /gene="dapC"
                     /locus_tag="WJL14_001876"
     CDS             1089..2276
                     /gene="dapC"
                     /locus_tag="WJL14_001876"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ7656905.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFKPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGDGYVRIALVVDVATCVKAAETIVS
                     LYR"
     gene            2538..3317
                     /gene="yaaA"
                     /locus_tag="WJL14_001877"
     CDS             2538..3317
                     /gene="yaaA"
                     /locus_tag="WJL14_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ7656906.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKKLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3400..3490
                     /locus_tag="WJL14_001878"
     tRNA            3400..3490
                     /locus_tag="WJL14_001878"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3434..3436,aa:Ser,seq:tga)
     gene            3703..3792
                     /locus_tag="WJL14_001879"
     tRNA            3703..3792
                     /locus_tag="WJL14_001879"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3737..3739,aa:Ser,seq:cga)
BASE COUNT          988 a          894 c          950 g         1006 t
ORIGIN      
        1 gcatattgaa tgcgggcaaa ccggctgctt tccgtttttg gatttcggag aatgccatcg
       61 cccagctttc atcacacata aaaaacagtg cgggcacggc ttttttcagc ggtatttctt
      121 tcaggtgcgg ggcaagtgcc gcccccatca ggatatgccg agaattaatc ataaaggtta
      181 cggtggcgat aagcagtatc ggcagaggtt ccgcccacag gttgaccgtg gcaaactcgg
      241 agccgccggc gaagttcata ctggtcatca acaacatttc cagccagctc atgccttttt
      301 gtccaccctg cataccgagt attaatgccc aaggcagaag tccgataagt atgggcgaac
      361 tttctttgat gccgcgtata aattcgttac ggggcgagct tatgtgtgtc atattataat
      421 attgatatgt aagaaggtcg ggcattatac acagacggag tatttcaaaa caaaacctat
      481 ttgctgtgtt tcagtgtaaa cacggcttgt gtataatcca ccatctttag acagaccgac
      541 cgtatgtggg agcaaggcaa tgaatattga agtagaaatg aaagtgttgg acgagaggat
      601 ggcggatttt gcccctgcct atgcgacggc gggttctgct ggtttggact tgcgcgcctg
      661 tttggatgag gaagtcgtct tgcagccggg ggaaacgttt cttgtgccga caggtttggc
      721 aatttatttg gccaatcctt catatgccgc cgttttgctg ccccgttccg gcttggggca
      781 taaacacggc attgttttgg gcaatttggt cggtttgatt gactccgatt atcaagggga
      841 attgaaggtg tcgttatgga acagaagcag cgaaccgttt actgtcaaac cgtttgagcg
      901 tatcgcgcag atggttatcg tgccaatcgt gcaggcgcgt ttcaaacgtg tcgaggaatt
      961 tgtcggaagc agccggggtg agggcggctt cggcagtacg ggtttgcatt gaatataaaa
     1021 tgccgtctga tgggtgccgt caggttcaga cggcatatct tccgatatac cgacaaccgg
     1081 agaacattat gaatacctta ctcaaccaac tcaaacccta tccctttgcc cgactgcgtg
     1141 aagcgatgca gggcatttcc gcccccgaag gtctggaagc cgtcccactg catatcggcg
     1201 aaccgaaaca tccaacaccg aaagtcatta cagatgcgct gaccgcctca ttgcacgagt
     1261 tggaaaaata tccgctgacg gccggtttgc ctgaactgcg tcaggcgtgt gcgaactggt
     1321 taaaacgccg ttatgatggg ttgacgctga attccgacac cgaaattctg ccggttttgg
     1381 gcagtaggga ggcgttgttt tcttttgtcc aaaccgtgtt gaaccctgtt tcagacggca
     1441 tcaaacccgt aatcgtcagc ccgaatccct tttatcagat ttatgaaggt gcgacacttt
     1501 tgggcggcgg tgaaatccat tttgccaatt gccccgcgcc gtctttcaaa cccgattggc
     1561 gcagtatttc cgaagaggtt tggaaacgca ccaaactggt gttcgtctgc tcgcccaaca
     1621 accccagcgg cagcgtgctg gatttggatg gatggaaaga agtttttgat ttacaggata
     1681 aatatggttt cattattgcc tcggatgaat gctattccga aatctatttc gacggcaaca
     1741 aacctttggg ctgcctgcaa gccgctgcac agttgggtcg aagcaggcaa aaactgctta
     1801 tgttcaccag tttgtccaag cggtccaatg ttccaggttt gcgttccggt tttgtcgccg
     1861 gcgatgccga actgcttaaa aactttctgc tttacagaac ctatcacggc agtgcaatga
     1921 gcattcccgt gcagcgcgca agcattgccg cttgggatga cgaacagcac gttatcgaca
     1981 accgccgcct gtatcaggaa aaatttgagc gcgttattcc cattttgcaa caggtatttg
     2041 acgttaaatt accggatgcc tcgttttaca tctggttgaa agtccccgat ggcgacgatt
     2101 tggcatttgc acgcaattta tggcaaaaag ccgctatcca agtattgccc ggacgttttt
     2161 tggcacggga taccgaacag ggcaatcccg gggatggtta tgtgcgtatc gctttggttg
     2221 tcgatgtcgc aacttgtgtc aaagctgcgg aaaccattgt ttccctatat cggtaaagaa
     2281 taaaaaatgc cgtctgaact tttgttcaga cggcattttt caatatttta cggttgaatt
     2341 tgctataacg gtatttatag tggattaaca aaaatcagga caaggcgacg aagccgaaga
     2401 cagtacagat agtacggcaa ggcgaggcaa cgctgtactg gtttaaattt aatccactat
     2461 acttcacttt taatcggctt gcccgcaaac acgtttaaac ttaaaatccc cgtgtttgac
     2521 acaataccga gcagattatg ttttttgtcc tttcccctgc gaagaacctt aatgaaaaag
     2581 accctgcccc tgtcagcgag tttacccaac ccgacctgct ggcagagtcc gacattctaa
     2641 tgcagcagtt gcgcgagctt gcgccgcaac agattgccga actgatgcac gtttccgaca
     2701 aaattgccct cttaaacgcg cagcgcaatg cagaatggaa cacgccgttt acgccggaaa
     2761 acgccaaaca ggcggtcttt atgttcaacg gcgatgttta cgaaggtatg gatgcaaaca
     2821 cattggatat tggacagata cgctatctgc aaaaccatgt ccgcctgctg tccggtctgt
     2881 acggccttct gcgtccactg gacctgatac agccttaccg tttggaaatg gggacggcat
     2941 ttgccaattt gcgcgggaag aatttgtatg agttttgggg agacatcatt accaaccttt
     3001 taaatgacac gcttgcccaa gcaggcagca atacgcttgt caaccttgcc tcacaggaat
     3061 atttcaagtc cgtcaacacg aaaaaacttc gggcgcggct gattaccccg atatttaaag
     3121 acgaaaaaaa cggcaaatat aaaatcatca gtttctatgc caagcgcgcg cgaggattaa
     3181 tggtgcgcta tgcggcagaa cacaatatta ccgatcctga aatgctgaaa aattttaatt
     3241 acgaaggcta cgcatttaat gacgcggctt caaatgaaag cgaatgggtg tttatgcgtt
     3301 cggaacaaat aaagtgaaaa caataaatta agtatttccc aaaaaaagtg cttggcaaaa
     3361 tgtataaact tcattattat tcctactctt caagaagacg gaagcgtggc agagcggttt
     3421 aatgcaacgg tcttgaaaac cgtcgagggt tgatagccct ccgtgagttc gaatctcacc
     3481 gcttccgcca atccttgagg tcaaaaacca aataaaaatg aagtagcata aagcatcggc
     3541 atttttattt ggttttactt catcgtgttt ccttttcggt tgaaccccac cctttagggc
     3601 ggtagaatca gactttattt gggaggggtg taaccctttc tgaatcaggg taacacatag
     3661 ggcggtgctt tatgtgtcgt cctgtgtgtt gaaacatctt tcggaaacgt ggcagagagg
     3721 ctgaatgcag cggactcgaa atccgctgag ggtgcaaatc ctccgtgggt tcgaatccca
     3781 ccgtttccgc cacaaaacaa aaccgccctg attcggggcg gttctttttt gttcaagt
//
LOCUS       DAWKQB010000163         3803 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.164, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000163 DAWKQB010000000
VERSION     DAWKQB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.164"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            354..3554
                     /gene="nalP"
                     /locus_tag="WJL14_001880"
     CDS             354..3554
                     /gene="nalP"
                     /locus_tag="WJL14_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ7656907.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGTSAPDFNAGGT
                     GIGSNSRATTAKSAAISYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHTG
                     DFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNEN
                     YKNYTAYMRKEAPEDGDGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIGG
                     RSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTTS
                     RAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFIF
                     SASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAPY
                     EASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAVG
                     VDSKFGWGLLDTGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGSQ
                     LQLHGNNTYTGKTVIEGGSLVLYGNNKSDMHVETKGALIYNGAASGGSLNSDGIVYLA
                     DTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAIIGGKLYMSARGKGAGYLN
                     RTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNAA
                     RTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGIR
                     PYGATFRAAAAVQHANAADGVRIFNNLAATVYADSTAAHADMQGRRLKAVSDGLDHNA
                     TGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSEN
                     SANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLMQ
                     LGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGL
                     KLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLG
                     ADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1031 a         1032 c         1006 g          734 t
ORIGIN      
        1 atagtacgga accgattcac ttgttaaaga atcgttctct ttgagctaag gcgaggcaac
       61 gccgtactgg tttttgttca tccactataa ctagggaaat tcaaattaag ttagaattat
      121 ccctatgaga aaaagccgtc taagccggta taaacaaaat aaactcattg agctatttgt
      181 cgaaagttca aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
      241 agacaacccc tataataata taattcaaaa tataaaaacg ggttacatct aaacattacg
      301 gaatttttat tccctcgcct gaattctatt gtcagattca aggagacctc atcatgcgaa
      361 cgaccccaac cttccctaca aaaactttca aaccggctgc catggcgtta gctgttgcaa
      421 caacactttc tgcctgctta ggcggcggcg gcacttctgc gcccgacttc aatgcaggcg
      481 gcaccggtat cggcagcaac agcagggcaa caacagcgaa atcagcagca atatcttacg
      541 ccggtatcaa gaacgaaatg tgcaaagaca gaagcatgct ctgtgccggt cgggatgacg
      601 ttgcggttac agacagggat gccaaaatca atgccccccc cccgaatctg cataccggag
      661 actttacaaa cccaaatgac gcatacaaga atttgatcaa cctcaaacct gcaattgaag
      721 caggctatac aggacgcggg gtagaggtag gtatcgtcga cacaggcgaa tccgtcggca
      781 gcatatcctt tcccgaactg tatggcagaa aagaacacgg ctataacgaa aattacaaaa
      841 actatacggc gtatatgcgg aaggaagcgc ctgaagacgg agacggtaaa gacattgaag
      901 cttctttcga cgatgaggcc gttatagaga ctgaagcaaa gccgacggat atccgccacg
      961 taaaagaaat cggacacatc gatgtggtct cccatattat tggcgggcgt tccgtggacg
     1021 gcagacctgc aggcggtatt gcgcccgatg cgacgctaca cataatgaat acgcatgatg
     1081 gaaccaagaa cgaaataatg tctgcagcca tccgcaatgc atgggtcaag ctgggcgaac
     1141 gtggcgtgcg catcgtcaat aacagttttg gaacaacatc gagggcaggc actgccgacc
     1201 atttccaaat agccaattcg gaggagcagt accgccaagc gttgctcgcc tattccggcg
     1261 gtgataaaac agacgagggt atccgcctga tgcaacagag cgattacggc aacttgtcct
     1321 accacatccg taataaaaac atgcttttca ttttttcggc aagcaatgac gcacaagctc
     1381 agcccaacac actgacccta ttgccatttt atgaaaaaga cgctcaaaaa ggcattatca
     1441 cagttgcagg cgtagaccgc agtggagaaa agttcaatgg ctccaaccat tgcggaatta
     1501 ctgccatgtg gtgcctgtcg gcaccctatg aagcaagcgt ccgtttcacc cgtacaaacc
     1561 cgattcaaat tgccggaaca tccttttccg cacccatcgt aaccggcacg gcggctctgc
     1621 tgctgcagaa atacccgtgg atgagcaacg acaacctgcg taccacgctg ctgacaacgg
     1681 ctcaggacat cggtgcagtc ggcgtggaca gcaagttcgg ctggggactg ctggatacgg
     1741 gtaaggccat gaacggaccc gcgtcctttc cgttcggcga ctttaccgcc gatacgaaag
     1801 gtacatccga tattgcctac tccttccgta acgacatttc aggcacgggc ggcctgatca
     1861 aaaaaggcgg cagccaactg caactgcacg gcaacaacac ctatacgggc aaaaccgtta
     1921 tcgaaggcgg ttcgctggtg ttgtacggca acaacaaatc ggatatgcac gtcgaaacca
     1981 aaggtgcgct gatttataac ggggcggcat ccggcggtag cctgaacagc gacggcattg
     2041 tctatctggc agataccgac cgatccggcg caaacgaaac cgtgcacatc aaaggcgatc
     2101 tgcagctggg cggcgaaggt acgctgtaca cacgtttggg caaactgctg aaagtggacg
     2161 gtacggcgat tatcggcggc aagctgtaca tgtcggcacg cggcaaaggg gcaggctatc
     2221 tcaaccgtac cggacaacgt gttcccttcc tgagtgccgc caaaatcggg cgggattatt
     2281 ctttcttcac aaacatcgaa accgacggcg gtctgctggc ttccctcgac agcgtcgaaa
     2341 aaacagcggg tagtgaaggc gacacgctgt cctattatgt ccgtcgcggc aatgcggcac
     2401 ggactgcttc ggcagcggca cattccgcgc ccgccggtct gaaacacgcc gtagaacagg
     2461 gcggcagcaa tctggaaaac ctgatggtcg aactggatgc ctccgaatca tccgcaacac
     2521 ccgagacggt tgaaactgcg gccgccgacc gcacagatat gccgggcatc cgcccctacg
     2581 gcgcaacttt ccgcgcagcg gcagccgtac agcatgcgaa tgccgccgac ggtgtacgca
     2641 tcttcaacaa tctcgccgct accgtctatg ccgacagtac cgccgcccat gccgatatgc
     2701 agggacgccg cctgaaagcc gtatcggacg ggttggacca caacgctacg ggtctgcgcg
     2761 tcatcgcgca aacccaacag gacggtggaa cgtgggaaca gggcggtgtt gaaggcaaaa
     2821 tgcgcggcag tacccaaacc gtcggcattg ccgcgaaaac cggcgaaaat acgacagcag
     2881 ccgccacact gggcatggga cgcagcacat ggagcgaaaa cagtgcaaat gcaaaaaccg
     2941 acagcattag tctgtttgca ggcatacggc acgatgcggg cgatatcggc tatctcaaag
     3001 gcctgttctc ctacggacgc tacaaaaaca gcatcagccg cagcaccggt gcggacgaac
     3061 atgcggaagg cagcgtcaac ggcacgctga tgcagctggg cgcactgggc ggtgtcaacg
     3121 ttccgtttgc cgcaacggga gatttgacgg tcgaaggcgg tctgcgctac gacctgctca
     3181 aacaggatgc attcgccgaa aaaggcagtg ctttgggctg gagcggcaac agcctcactg
     3241 aaggcacact ggtcggactc gcgggtctga agctgtcgca acccttgagc gataaagccg
     3301 tcctgtttgc aacggcgggc gtggaacgcg acctgaacgg acgcgactac acggtaacgg
     3361 gcggctttac cggcgcgact gcagcaaccg gcaagacggg ggcacgcaat atgccgcaca
     3421 cccgcctggt tgccggtctg ggcgcggatg tcgaattcgg caacggctgg aacggcttgg
     3481 cacgttacag ctacgccggt tccaaacagt acggcaacca cagcggacga gtcggcgtag
     3541 gctaccggtt ctgacggaca ggaagcagac agccgcaaag atcacggtct ttgcggctgt
     3601 ttcttatgaa aagaaaaccc tattccaatt gcctgcttct attgtttcaa gacttcttcc
     3661 aaagattcgg catcaatcag atgtatagcg gattaacaaa aatcaggaca aggcggcgaa
     3721 gccgcagaca gtacaaatag tacggaaccg attcactcgg tgcttgagca ccttagagaa
     3781 tcgtttcttt gagctaagtc gag
//
LOCUS       DAWKQB010000164         3793 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.165, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000164 DAWKQB010000000
VERSION     DAWKQB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.165"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="WJL14_001881"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="WJL14_001881"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ7656908.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKIGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGKACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPSGFGIRVDSHIYQGYRIPPYYDSLIGKVCVVG
                     KDRDQAMAKMRVALAELAITGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1573..2028)
                     /locus_tag="WJL14_001882"
     CDS             complement(1573..2028)
                     /locus_tag="WJL14_001882"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ7656909.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2010..2267)
                     /locus_tag="WJL14_001883"
     CDS             complement(2010..2267)
                     /locus_tag="WJL14_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ7656910.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            2384..3424
                     /gene="queA"
                     /locus_tag="WJL14_001884"
     CDS             2384..3424
                     /gene="queA"
                     /locus_tag="WJL14_001884"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ7656911.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSHLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT          792 a          928 c         1159 g          914 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacctggg
      181 tcgttgaaca agtcgcggtg cagcggcgta ttggttttga tgccggtaat cgccagttcg
      241 gcaagggcta cgcgcatttt agccatggct tggtcgcggt ctttaccgac gacgcagact
      301 ttgccgatca ggctgtcgta gtaaggaggg atgcggtagc cttggtaaat gtggctgtcg
      361 acgcggatac cgaagccgct gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtatg ggtcttcggc gttgatacgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacaatg
      541 tccacgccgg tgatgagctc ggtaaccgga tgctcaacct gaacgcgtgt gttcatctcg
      601 ataaagaaga actcgccgtc ttcgtacagg aattcaaacg tacctgcgcc gcgatagccg
      661 atgcgtttgc aagcgtcggc acaggctttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaagat atacggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgatttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cgggtggaca gcgtccgcgc agcttacttc ggcggcggca
     1261 ataatggcgg ggacgttgag gtagctttgt gcagatgcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtgattc cggcaacccg tccgtaaaaa
     1501 aaaggtgcgg gaacgccgaa gggggaaagc atttcagacg gcatcggaag tttatgccgt
     1561 ctgaaaacag gattatccga taatgaagag cggttcgccg aattcgacgg gcgtaccgtt
     1621 ttcgaccaaa atttctttga ccgtgccgga tttttcggct tcgatttcgt tcatcagctt
     1681 catcgcttcg atgatgcaca gcgtgtcgcc ggctttaacc tgttggccga cttcgacaaa
     1741 aggcgcggca ttcgggccgg gtgcgcggta gaacgtgccg accataggtg atttttgcgc
     1801 gtcggacaaa tcgcgggcgg caggtgcggc ggcaggcgcg gatgccgcaa cgggtgcggc
     1861 ggcaggcgtt acggccggcg cggcggcagg tacgggcgcg gcgtaaacgg gtgcggcggc
     1921 gatggttcgg gtgatgcgga ctttttcctc gccttcggtt acttcgattt cggcgatacc
     1981 cgattcttca accaaatcaa tcagtttttt taatttgcgc aaatccattt ctgttccttt
     2041 aaaggctgcc ggtgtttcgg cgggttgttg cgttggtttt tcagaaaaca gccggcttgc
     2101 ggcaacggcg caaaccgggc ggaatggtgt tcggatacgg ttttcctgtg gtggaaaaca
     2161 accgtgagaa tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa aatatcaaaa
     2221 aacggcggtt tttgggggaa atgtgcagga agtttgattt tgcgcacaag atgccccggg
     2281 tcaagagtgt ttattctaat ctgttggttt ttcgggcaaa gatgccgtct gaaaaagggc
     2341 taaagtgcgt ataatggcgg cttgcccgaa cgagagtgta aaaatggata tttcagattt
     2401 tgattttacc ctgcccgaac acctgattgc ccagcatccg cccgaagtgc gcggcagcag
     2461 ccaccttttg gtcgcgctgc ccgatatgcc gctgcaagac cgggtgttcg gcgatttgcc
     2521 ggattatgtc gaggcgggcg acgttttggt gttcaacaac accaaagtca tgaaggcgcg
     2581 gctgtttggg cagaaagaca gcggcggcag gatcgaagcc ctgatcgagc gtgttttaga
     2641 cagtcatacc gcactggcgc acatccgttc gtccaagtcc cccaagcccg gtatggggct
     2701 ggtgtttgaa ggcggcatcc gcgccgtgat ggtcgggcgt gagggcgaac tgttctgcct
     2761 gcgttttgaa ggcggagaga ccgtttacga acttttggaa cagaacggac acctgcccct
     2821 gccgccttat atcgaacgtg ccgccgatgc ggacgacgac agccgctacc agactgttta
     2881 tgccaaatat cagggcgcgg tcgccgcgcc gacggcgggc ctgcatttta cggaagaact
     2941 tttgcgccgt ctgaaagaca aaggcgcggt aaccgcagaa gtaaccctgc acgtcggtgc
     3001 gggaacgttc caacccgtgc gcgtcgataa aatcgaagaa cacaaaatgc acagcgaatg
     3061 gtttgaagtg ccgtctgaaa ccgccgccgc cgttgaggcg gcaaaagccc gggggaacaa
     3121 agtctgggcg gtcggcacga cttccatgcg cgccctcgag tctgccgcgc gtgcgacggg
     3181 tcgtctgaaa gcggggcagg gcgacaccga tattttcatc acgccgggct accgttttaa
     3241 tgttgccgac aggctggtta ccaatttcca tctgccgaaa tcgacgctgc tgatgcttgt
     3301 cagcgcgttt tcgggtatgg gtcatatccg cgccgcgtat cgttatgcgg tcgaacgcga
     3361 ataccgtttc ttcagctacg gcgatgcgat ggttttgggg cggaacgaag gggtggtacg
     3421 ttagaacagc ctcaagtcta ttgggggaag acaagtgccg tctgaacgga cgttcggacg
     3481 gcattctttt gttgatttga atgaaaggaa ctgtatatga catagattca ggcgcgagag
     3541 gcgagaaact cagtcattgt tgatgacgcg caatacgtta aaattcattc cgttttggtc
     3601 gtataaacat attacatacg ccattttttg tattcaaggc tgcctgaaaa cagtgggcaa
     3661 gaatatctaa cccaacgaaa agctgaaaaa gcaacaaaaa ataatctgca ccttagtcgg
     3721 ttggttagtc caaccttagg ggtgcagacc attcaatctt tcaggctgcc tttgtttgtt
     3781 tataagataa att
//
LOCUS       DAWKQB010000165         3750 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.166, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000165 DAWKQB010000000
VERSION     DAWKQB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.166"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(88..906)
                     /locus_tag="WJL14_001885"
     CDS             complement(88..906)
                     /locus_tag="WJL14_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7656912.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPDPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIGNPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDVDIFRNFSL"
     gene            complement(986..2236)
                     /locus_tag="WJL14_001886"
     CDS             complement(986..2236)
                     /locus_tag="WJL14_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ7656913.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDADDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJL14_001887"
     CDS             complement(2233..2694)
                     /gene="tsaE"
                     /locus_tag="WJL14_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ7656914.1"
                     /translation="MSDLPSISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2896..3708
                     /locus_tag="WJL14_001888"
     CDS             2896..3708
                     /locus_tag="WJL14_001888"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ7656915.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          837 a          909 c         1112 g          892 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcggaaaa tatccacatc
      121 aggctgggga ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggcg aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatacagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaagg gatttcctat ttttggggct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttggataa aggcgtgtag tctgtcccgc ttatagtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttccg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggatcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactgt acacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gtccgttaaa acagataaag gaagccgatc ggacaattcg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacaa
      961 ctatattgaa acaccgcctt ttcaatcaac cccgcttcaa tacggatgta ttgatgtagc
     1021 gttggacacc cgaggcaatg gattgggcgc actgccgacg gaaggattcg ctgcccagca
     1081 gcttctcttc ggcaggattg gacaggaagg cggtttcgac caggatagac ggcatatcgg
     1141 gcgcgcgcaa aacggcgaaa ttggcttcgt ccaccctgcc tttgtgcaag tggttgagcc
     1201 tgcccaattc ttcaagcacc agtttgccga gtttgcggct gtcgcgcagc gtggcggttt
     1261 gggtcatatc gagcagggcg gtatcgacgt tgcggttgcc gctggtcggt acgccgccga
     1321 ccgcgtcggc attgttttgc gtctgttcca agaatttggc ggcggagctg gtcgcgcctt
     1381 tggtgttcag catataaacc cctgtgccgc gcgcggaggg gctagtgaaa gcgtcggcgt
     1441 ggatggagac gaatacgtcc gcccgccgtg ctcgcccttt ggcgacacgc acgcccaagg
     1501 ggatgaacac gtcttcgttg cgcgtcataa agacgttgta acccaaggct tccaattgtt
     1561 ttttggtttc gcgggcgatg gagaggacga cgtgtttttc ctgcaaaccg cccgggctga
     1621 cggcgccggg gtcttcgccg ccgtgtccgg ggtcgatcat aatgacgggt ctgcgcccgt
     1681 ttttgccgcg ttcggtttgt gcgggagtgt tttgagcgag gtcggcttca ggggagccgc
     1741 gacgggtttt gttcaggctg ccgttgagca gcgccatcat cggatcgtcg gcatccatcc
     1801 cttgcggata gagatcgacg acgaggcggt ttttaaagcc gccgacgggg gggagggaaa
     1861 aaacttgcgc gtaggaaggc tgtttcaaat cgatgacgag gcggacggtg tcgggcgtgt
     1921 tctgaccggc gcgtatattg cggataaagg ggtcgtccgc cataaccttc tgagacagtc
     1981 cgtgcaatac ggtattgatg ttcgcgtttt gtatgtcgac gaccaacctg cccgggttgt
     2041 cgagcgtgaa gtgttggtat ttgagcgcgg ttgtgctttc cagcgtcagg cgggtgtagg
     2101 tgtgcgacgg ccatatccgt acggcggtaa actgcggggc gcgtaccgtt ttggcaacgg
     2161 cggatgcgat ggggcttagg gcgaacagtg tgccggcggt gcggcggatg atttgtcttc
     2221 gtgtcagttt ggtcatagcg gcaggctttc gcgtcctcgt tcggtatggg cggtcagcag
     2281 gcattttctg ccgccgccgt cgtgtgtcaa tgttgcggtg atgtcggcgg gcggggtaaa
     2341 ttcgcctccc tgttgcggcc attcgatcag gcagacgctg tttgcggcaa acagttcgtc
     2401 aagccccgcg tcttcccatt cttcggggaa cgagaagcgg tagaggtcga aatggtgcag
     2461 ggtgaagcgt tccagcggat aagattcgac gatggcgtag gtcggacttt tgactgcgcc
     2521 ctgatgaccc aatccgcgca ggatgccgcg tgtcagcgtg gttttgcccg cacccaaatc
     2581 cccttcgaga taaatgacca gcggtgcgtt taaacgggaa gaccacgccg cgcccaaatc
     2641 gagtgtggcg gcttcgtcgg caaggaatcg ggagatagag ggtaaatcag acatggaaac
     2701 ggtttgttgt aaggtctagg gtattatggg cagttttgca ggttttgcaa actttgcacc
     2761 cgaggggcgg atgcttcttg tccgagcatt ataacagcca aatccgcgtt ctgctttcag
     2821 acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt atgtttagga
     2881 cggattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt ttttgactcg
     2941 ggaatcggcg gtttgaccaa tgtacgcgcg ctgatggagc ggctgccgat ggagaacatc
     3001 atttatttcg gcgacacggc gcgcgtgcct tacgggacga aatctaaggc gaccatcgaa
     3061 aatttctcga tgcagattgt cgatttttta ttggaacacg atgttaaggc gatggttatc
     3121 gcgtgcaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac gggcaatatg
     3181 cctgtgttgg acgtgatttc cgccggcgcg aaagccgcgc tagcaaccac gcgcaacaat
     3241 aaaatcggca ttatcgccac caataccaca gtcaacagca acgcctatgc gcgcgccatc
     3301 cataggaaca accccgacac gctcgtccgc acgcaggccg cgccgctgct cgtccctttg
     3361 gtggaagagg gctggttgga acacgaagtt acccgcctga ccgtatgcga atacctcaaa
     3421 ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt tcccttgctc
     3481 aaacccttaa tcggcaggga ggcgggcaat gtcgcgttgg ttgattctgc aattacaacg
     3541 gccgaagaaa ccgcacgcgt ccttgctcag gaaggattgc tcaataccga caacaacaat
     3601 cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat cggcgagcgt
     3661 tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa cgatgacgga
     3721 aagctgcccg agattacaga aacctaaaat
//
LOCUS       DAWKQB010000166         3675 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.167, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000166 DAWKQB010000000
VERSION     DAWKQB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.167"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            154..327
                     /locus_tag="WJL14_001889"
     CDS             154..327
                     /locus_tag="WJL14_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ7656916.1"
                     /translation="MKSERRHWEWCVDYDGEGVVRDYRFTHKVEKDERSVNRDAVGVI
                     RKEAGKSLSQPEK"
     gene            391..1674
                     /gene="hemL"
                     /locus_tag="WJL14_001890"
     CDS             391..1674
                     /gene="hemL"
                     /locus_tag="WJL14_001890"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ7656917.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAVREAALGGLSFGAPTEGEIAIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1890..2162)
                     /locus_tag="WJL14_001891"
     CDS             complement(1890..2162)
                     /locus_tag="WJL14_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ7656918.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2173..2736)
                     /gene="orn"
                     /locus_tag="WJL14_001892"
     CDS             complement(2173..2736)
                     /gene="orn"
                     /locus_tag="WJL14_001892"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ7656919.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPRRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="WJL14_001893"
     CDS             complement(2754..3641)
                     /gene="prmA"
                     /locus_tag="WJL14_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ7656920.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGEILEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEDTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGFAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
BASE COUNT          763 a          942 c         1070 g          900 t
ORIGIN      
        1 acggaagagg gaatcgcaca aacggttgtt aaaggcaaaa cgacccgggc ggaggtagag
       61 gcacgtttcg ggaagcgcaa ccctttcggt tgttatgcct atcatgaagt cagcctgccg
      121 atttataatt ttttgccgac caatttcatt tatatgaaat cagaacgccg gcattgggag
      181 tggtgtgtgg attacgacgg ggagggagtt gtccgggact accgctttac acataaagtg
      241 gaaaaagacg aacgttccgt caaccgggat gcggttggcg tgatccgcaa agaagcgggc
      301 aaatccttgt cgcaacctga aaaatgataa aatggggctt tctgcttcca agcccgaaac
      361 ctgccgttca ggcggcattt gaggataaat atgaaccgta acgaaatttt attcgaccgc
      421 gccaaggcgg tcatccccgg cggtgtgaat tcgccagtcc gcgcattcgg cagcgtcggc
      481 ggcgtgccgc gcttcatcaa aaaagccgaa ggcgcgtatg tttgggatga aaacggcacg
      541 cgctacaccg actatgtcgg ctcgtggggg cctgcgattg tcggccacgc gcaccccgaa
      601 gtcatcgaag ccgtgcgcga agccgcgttg ggcggtttgt cgttcggcgc gcctaccgag
      661 ggcgaaatcg ccattgccga agaaatcgcc aaaatcatgc cgtccgtcga acggctgcgc
      721 ctcgtcagct ccggtaccga agcgaccatg accgccatcc gtctggcacg cggctttacc
      781 ggccgtgaca aaatcatcaa atttgaaggc tgctaccacg gccattccga cagcctgttg
      841 gtgaaagcag gcagcggtct gctcaccttc ggtaatccct cttccgccgg tgtgcctgcc
      901 gactttacca aacacacttt agtcctcgaa tacaacaaca tcgcccaact cgaagaagcc
      961 tttgctcaaa gcggcaatga cattgcctgt gtgattttgg agccttttgt cggcaatatg
     1021 aatctggtta ggccgtctga agccttcgtc aaagccctgc gcgaattgac cgaaaaacac
     1081 ggcgcagtgt tgatttacga cgaagtgatg accggtttcc gcgtcgcgct cggcggcgca
     1141 caatcgctgc acggcatcac gcccgacctg accacgatgg gcaaagtcat cggcggcggt
     1201 atgccacttg ccgcgttcgg cggacgcaaa gacattatgg aatgtatttc cccgctgggc
     1261 ggcgtgtatc aggcaggcac cttgtcgggc aacccgattg ccgtcgccgc cggtttgaaa
     1321 acgctggaaa tcatccgccg cgaagggttt tatgaaaacc tgacggcgcg taccgaacag
     1381 ttggttcaag gttttcggac ggcagcggat gcggcgggca tcgagttcac cgccgacagc
     1441 gtgggcggta tgttcggtct gtattttgcc gcacacgcgc cgcgaaatta tgccgatatg
     1501 gcgcgttcca atattgaagg cttcaaacag tttttccacg ggatgctcga ccgcggcatt
     1561 gccttcggcc cgtccgctta tgaagcaggt ttcgtttctg ccgcgcatac gcccgagctg
     1621 attgacgaaa cggttgcggt tgcggttgaa gtgttcaagg cgatggctgc atgatgtttt
     1681 gacggacaga gtttctctgt tcgatttgtt tggcagattg aagtaagaat gcacaccgcc
     1741 gtcattcccg cgcaggcggg aatccagacc tttcagtttc tgtaatgatt gaaaataacg
     1801 ggccgaaatc agccgcgccc ggcaggaagc cgccgccacc tgaaacaatg ccgtctgaag
     1861 ccttcagacg gcattttatc ggaagcgcat cagaaaaaac ggatagcgac atatgcggta
     1921 aaagcaaggg caattacata gataaacccc tttataaatt caaaagtctc atctatatcg
     1981 ctatattttt ctttcagttc gtcagatttt cgataacgtt tcattttcaa gaccacttca
     2041 tgaccaacaa gcccgacctt ccggattccc gcctgcgcgg gaatgacggg cgtgtatatt
     2101 tttgatttca atctgctgta aaaatgccgt ctgaaatgca ggatttgcgc ttcagacggc
     2161 attttttgtt tcttattgcg cttcggctct cggggcggaa atcagaaagt gttcgcggta
     2221 gtggcgcatt tcttcgatgc tctccaaaat gtcgtccaat gccttgtgcg aaccgcgttt
     2281 gacgacgctt ttggcaacgg gcggattcca gcgtttggca agctctttca gtgtggaaac
     2341 gtcaaggttg cggtagtgga agtagttttc cagtttcggc atatatttga ccataaaacg
     2401 ccggtcttgg tggatggaat tgccgcacat cggcgtggcg cgtctgggta cccattccga
     2461 cataaagtcc agcagtttct gttcgacttc ggcttcggta tgcgacgatt cgcgtacgcg
     2521 ctgtgtcagc cccgtcctac cgtgtgtggc ggtgttccat tcgtccatat tgtcgagcag
     2581 ctcgtcgctt tggtggatgg cgtaaacttc ggattgcgcc aatacattca aatccgagtc
     2641 ggtaataatc atcgcgactt cgataatgcg gtcggtttcg ggattaagcc ccgtcatttc
     2701 catatcgagc cagcataggt tgtttttatc ctgcatgatg tttcctttgc ttgtcagcgt
     2761 tttgtcccgc tcagtcgcgc ccatccttct tctgtttccg caggatcgag atcgaaccat
     2821 tggctgtaaa tgccgccgag ttcttcggct tgttcgtcca acaaaccgga taacacgatg
     2881 cggccgcctt gtttggtgcg ggcggcgagc atttcgccga gcatacgtaa agggttggcg
     2941 aggatgttgg cgacaactac gtcgaattgc ccttgaggca gaccgtcggg caggaagaat
     3001 tgtgcatcga cgttgttctg ttcggcgttg tccttgccgg cgcggacggc ttgttcgtca
     3061 atatccacgc cgacggcgaa acctgcaccg agtttgaggg cggcgatggt caggatgccc
     3121 gaaccgcagc cgtagtcgag gacgctttcg ccgtttttga gttgcgtatc cagccatttg
     3181 aggcagaggc gcgtggtcgg gtggctgccg gtgccgaagg cgaggccggg atcgaggcgg
     3241 aggttgacgg cagtgtcttc gggggcttcg tgccaagagg gggtaatcca caggcgttcg
     3301 gaaattcgga tggggtcgaa ttgagcttgg gtcaggcgta cccagtcttg gtcttcgagg
     3361 atttcgccgg tgtatgccaa gtcttttaat ccgcattctt gtgcggcggt ttggatgatg
     3421 gcggcggctt cgtcgtgttc gccgaacagg gcgatgactt tgctctgctg ccagatttgt
     3481 tcggcgggca taccgggttc gccgaaaatc gcctgttcgt tttgcgtgcc ggcgtaggca
     3541 tcttcgatgg cggcggagag tgcgccgtgt tccatcagcg cgtcggcgag gcgttcggcg
     3601 acggcatcgt tgacgttgac ggtgatttgt tggtagggca taacgggctt tcttggaaat
     3661 atagtggatt gaatt
//
LOCUS       DAWKQB010000167         3646 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.168, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000167 DAWKQB010000000
VERSION     DAWKQB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.168"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            207..1328
                     /gene="dnaJ"
                     /locus_tag="WJL14_001894"
     CDS             207..1328
                     /gene="dnaJ"
                     /locus_tag="WJL14_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ7656921.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1523..3541
                     /locus_tag="WJL14_001895"
     CDS             1523..3541
                     /locus_tag="WJL14_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ7656922.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
BASE COUNT          766 a          815 c         1092 g          973 t
ORIGIN      
        1 ataacaaaaa cacttcatgg cggttggttt tatgcctgtc taagtttttg tgtcgtgcat
       61 acctgaagat ttcagacggc atcggtttat gctgtctgaa aagtgtattc cgtttcagtt
      121 tgtaagctat ggcagtctgt ttgtcttgtg ttttgcgtaa ttgcccttat tttaagccgt
      181 gattttattt tgaattagat gaaaaaatga gtaatcaaga tttttatgcg acgctgggtg
      241 tggcaagaac agctaccgat gatgagatta aaaaagccta ccggaaattg gcgatgaaat
      301 atcatcccga ccgcaatcct gacaataaag aggcggaaga gaagtttaaa gaagtacaaa
      361 aggcgtatga aactttgtcc gacaaggaaa agcgcgctat gtacgaccag tatggtcatg
      421 cggcgtttga aggcggcgga caggggggct tcggagggtt tggcggattt ggcggtgcgc
      481 agggttttga ctttggggat attttcagcc aaatgtttgg aggcggttcg gggcgcgccc
      541 agcctgatta tcagggtgag gacgttcaag tcggtatcga aatcacgctt gaagaagccg
      601 caaaaggtgt gaagaaacgc atcaatattc cgacttatga agcgtgtgat gtctgtaacg
      661 gcagtggcgc gaaaccgggg acatccccgg aaacctgccc gacttgcaaa ggttcgggta
      721 cggtgcacat ccagcaggcg attttccgta tgcagcagac ttgtccgacc tgccacggtg
      781 cgggcaaaca cattaaagaa ccttgcgtca aatgccgtgg cgcggggcgg aataaggcgg
      841 tcaagacggt ggaagtcaat attcccgccg gtatcgatga cgggcagcgt atccgtttga
      901 gcggcgaagg cggaccgggt atgcacggtg cgcctgctgg cgacctgtat gtaaccgtcc
      961 gcattcgggc gcataagatt ttccaacgcg acggtctgga cttgcattgc gaactaccga
     1021 tcagttttgc cacggctgct ttgggcgggg agttggaagt gccgaccttg gacggaaagg
     1081 tcaagctcac cgtccccaaa gaaacccaaa ccggcaggag gatgcgcgtg aagggtaagg
     1141 gtgtcaaatc tttacgcagc agcgcgaccg gcgatttgta ctgccatatt gttgtcgaaa
     1201 cgcctgtcaa tttgaccgac cgtcaaaaag agcttttgga agaatttgag cggatttcta
     1261 ccggcttgga aaaccaaaca ccgcgcaaga aatcgttttt agacaagctg cgcgatttgt
     1321 ttgattgatt ttaaggttcg gaaacaagca gccgtatcgg ggaatctccc tgatacggct
     1381 gttttgtttg tttaaaaata gtttttattt tcaatggggt atggggcagg gtgggataac
     1441 tgtttttaac tgttcttttt aaaacttgac atcatggcgc gatgccaaca atatgtgaac
     1501 gtctgttgtc aaaggaagaa taatgaataa atctttatcc gggtcggtag aagaataccg
     1561 cgaactgacg ctccgaggca tgatactcgg tgcattgatc actgtaattt ttactgcgtc
     1621 caacgtttac ctcggtttga aagtcgggct gacctttgcc tcgtcgattc cggcggcggt
     1681 aatttcgatg gcggttttaa agtttttcaa aggcagcaat attttggaaa acaacatggt
     1741 gcagacccaa gcttcggcgg cgggtacgct ttcgaccatc atcttcgtcc tgcccggttt
     1801 gctgatggcg ggctactgga gcggtttccc gttctggcag acgacgcttt tatgtattgc
     1861 cggcgggatt ttgggggtga ttttcaccat tccgctgcgt tacgcgatgg tggtgaaaag
     1921 cgatttgcct tatccggaag gtgtggcggc tgctgaaatt ttgaaagtgg gcggtcatga
     1981 agaaggggat aaccgtcagg gcggcagcgg catcaaagag ctggcggccg gcggtgcgtt
     2041 ggcgggattg atgagctttt gcgccggagg tctgcgcgtg attgccgaca gcgcgagtta
     2101 ttggtttaaa agcggtacgg cgattttcca gctgccgatg ggcttttcac tggcattgtt
     2161 gggcgcgggc tatttggtcg gactgacggg cggtatcgcc atcctgttgg gcatttcgat
     2221 tgcttggggc attgccgtgc cgtatttctc ctcacacatt ccgcaacctt ccgatatgga
     2281 aatggcggcg tttgcgatga agctgtggaa ggagaaagtg cgttttatcg gtgcggggac
     2341 tattggcatt gcggcggttt ggacgctgtt gatgctgctc aagccgatgg tggaaggcat
     2401 gaagatgtcg ttcaagagtt ttggcggcgg tgcgcccgct gcggaacgcg ccgaacaaga
     2461 tttgtcgcct aaagccatga ttttttgggt gctggcgatg atgtttgttt taggcgtgtc
     2521 gttttaccac tttatcggcg attcgcacat tacgggcggc atggcttggc ttttggtggt
     2581 cgtttgcacg cttttggctt ccgtcatcgg ctttttggtc gccgccgcct gcggttatat
     2641 ggcaggtttg gtcggctcgt cttccagccc gatttccggc gtgggcatcg tgtccgtcgt
     2701 cgttatttca ctggttttgc tgctggtagg cgaatccgga ggtttgttgg cggatgaggc
     2761 taaccgcaaa tttttgctgg cactgacttt gttttgcggc tcggcagtaa tctgcgtggc
     2821 ttcgatttcc aatgacaacc tgcaagactt gaaaaccggc tacctgctca aagccacgcc
     2881 ttggcggcag caagtcgccc tgattatcgg ctgtatcgtt ggtgcgctgg ttatttcgcc
     2941 cgtgttggaa ctgctttacg aagcctacgg ctttaccggc gcaatgccgc gcgaaggcat
     3001 ggatgcggcg caggctttgg cagctcctca agcgactttg atgacgacca tcgcgtcggg
     3061 cattttcgcc cacaacctcg aatgggtcta catctttacc ggcatcgcga tcggcgcggt
     3121 attaatcgtc gtcgatttgg tgttgaaaaa atcatcaggc ggcaaacttg ccctgcccgt
     3181 ccttgcggtc ggtatgggta tttatctgcc gccgtccgtc aatatgccca tcgtggcagg
     3241 cgcggtgttg gcggcggtgt tgaaacacat catcggtaaa aaagcggaaa accgcgaagg
     3301 ccgtctgaaa aacgccgagc gtatcggaac cttgttctcc gccggcctga ttgtcggtga
     3361 aagcctgatc ggtgtgatta tggcgtttat tattgccttc tccgtgacca acggcggctc
     3421 ggatgcgccg cttgcgttga atctgcaaaa ctgggatgcc gccgcttctt ggttgggttt
     3481 ggcgttcttc gttaccggga tgtttttctt tgcacagcgc gtactgaagg cgggcaagta
     3541 ggctgtcgga aaaaatgccg tctgaagcgt tcagacggca tttttatctg gaaagcggaa
     3601 ggcggagctt ttcggcttgc gcccacgttt tgccggcaag gtcttt
//
LOCUS       DAWKQB010000168         3567 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.169, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000168 DAWKQB010000000
VERSION     DAWKQB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.169"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(120..974)
                     /locus_tag="WJL14_001896"
     CDS             complement(120..974)
                     /locus_tag="WJL14_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ7656923.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFSKENGTEVISFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(1103..1831)
                     /gene="rpsB"
                     /locus_tag="WJL14_001897"
     CDS             complement(1103..1831)
                     /gene="rpsB"
                     /locus_tag="WJL14_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ7656924.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(2032..2241)
                     /locus_tag="WJL14_001898"
     CDS             complement(2032..2241)
                     /locus_tag="WJL14_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656925.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            2319..2900
                     /locus_tag="WJL14_001899"
     CDS             2319..2900
                     /locus_tag="WJL14_001899"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ7656926.1"
                     /translation="MRNEEKHALRRELRRARAQMGHQGRLAAGQTINRLLKRYIKRGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQFVDRLPREPHDLLLDGFVSEAGILCF"
     gene            2961..3551
                     /locus_tag="WJL14_001900"
     CDS             2961..3551
                     /locus_tag="WJL14_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ7656927.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT          770 a          831 c         1036 g          930 t
ORIGIN      
        1 ttttgttttc agacggcctt tatattcggt cgtcttaaaa taggtaccgt aaacggtttt
       61 ctcaaaaaaa agcaccctga ttcgtttgga atccaggtgc tttctttttc ataagtgcct
      121 tacactttag cagcggcagc aacttcggct gcgtaatcga cggctttttt ctcaatacca
      181 tcgcctactt tgtagcgtac gaagctgatc acttcagtgc cgttttcttt agagaattgg
      241 gcaacagttt gatcggggtt catcacgaat gcttggccgt tcagagtgat ttcagccaag
      301 aatttacgga tgcggccttc aaccatttta gcggcgatgt cggcaggttt gccggaagcg
      361 atggcttgct cggtgtagat gtggcgttct ttttcaacgg tttcggcatc tacttcggct
      421 tcgcttacgc attgtggttt agcggcaaca atatgcatac cgattttgcg tgctacgtct
      481 tcagagcctt tgtactcaac caatacgcct tcggtcgcca atgcgccgtg gatgtaggca
      541 accagttggt tggcagtgtc gatcacttgg aagcgacgga cagacatatt ctcgcccaat
      601 ttggcgatga tggctttgcg ttctgcttca accagttcgc tcagttcttc aacagaagcc
      661 ggttttttct cggcagcagt tttcgcaacg aagttggcaa attctacgaa gcccgcgtct
      721 ttagcaacga agtcggtttc gcagtttact tcgaccaatg cgccgacatt gccgttgatc
      781 gcgtaagcca atacgccttc ggcagcggta cggccggcca gtttaccggc tttcgcaccg
      841 gatttgatac gcaggatttc ttcggctttg tcgaagttgc cttcggcttc aaccaaggct
      901 tttttgcatt ccatcatgcc caggccggta gcggcgcgca ggtcggcaac catttttgca
      961 gtaatttctg ccattttgaa tctcctagat ttttgggaaa tacggaaagg attttccgca
     1021 ttcgggaatc gtgtgccgcg aacggacgtt cagacggcat atttgagaaa aggggcataa
     1081 cgcccctctt cggtttgccg gattactcgg cagcggcttc ttgggcagcg gctacggttt
     1141 cttgcagcgc ttggtttttg ccttccaaaa ctgcgtcagc gatgccgcgg cagtacaggc
     1201 ggatggcttt ggcggagtcg tcgttgccgg ggataacgta tttcacgccg tcggggctgt
     1261 tgttggtatc gactacggcg ataacaggga tgcccaattt ttcagcttca accagagtac
     1321 ctttttggta gccggtatcg ataacgaaaa tcgcgtcagg caggcctttc atgtttttga
     1381 taccgcccaa agaacgttcc agtttttcaa catcgcgttg catttccaga atttcttttt
     1441 tgctgaaacc gctttcggca gcattttcca aggctgcggt tttttcttcc aggcgtttga
     1501 tggattgctt aacggttttg tagttggtca gcataccgcc caaccagcgg taatcgacga
     1561 aaggcatacc ggcgcgggtc gcttcttcgc ggatgatgtc gcgggcttgg cgtttggtac
     1621 ctacgaacaa tactgtacct ttgttggcaa ccaaacgacg tacggcttct tgcgcgtctt
     1681 ggaacatcgg cagggttttt tccaggttga cgatgtggat tttgttgcgc gcgccgaaaa
     1741 tgtattgtgc cattttcggg ttccagaaac gggtttggtg gccgaagtgg acaccggctt
     1801 caatcatctg acgcatagta atttgagaca ttttgatttc cttgaaaggg ttaagggcac
     1861 acattcaatc gcatagaact tgatagaccg cgcctattgt caagcaccct tcggcgaaag
     1921 tgggcataag ttttaaaaaa taaaagagtg tttccgaaat ggaaaactga cggattatag
     1981 cggataagag gtcgtctgaa aagggattca gggttttgtg ccgctgtctt tttaccgttg
     2041 tgcacgctgc tgttcgaatt ctgcctgttc ctgttgcttc atcaggcggt tgcggcggcg
     2101 gatggtcacg acaaagaccg tgaacacggt gggcagcacc gcccaaaaaa ccaaataaat
     2161 caaggcgcgc gcaatactcg gctgcgcggc ggaaaacatc acggcaacaa aaagatagcc
     2221 gatggcgacg atgtgccaca ttgtgtgcgt cctgtttgaa tgttaacaaa atcgttataa
     2281 tcgcaacatt ctaccgcaat cccgaaaggc cgtctgaaat gaggaacgag gaaaaacacg
     2341 ccttgcgccg agagttgcgc cgcgcccgcg cgcagatggg gcatcaaggg cggttggcgg
     2401 cggggcaaac gattaaccgc ctgctcaaac gttatatcaa gcgtggtcgg aaaatcggcg
     2461 tgtattggcc gatgggcaag gaattgcgtt tggacggctt tgtccgcgcg gcgcaaaaac
     2521 gcggtgcaaa actttatctg ccttatatcg aaccgcgttc gcggcggatg tggtttacgc
     2581 cgtatcctga aagcggaatg gaacgggagc gcatacgggg cagggcgaag ttgaacgtgc
     2641 cgcagtttgc agggcgcaaa atccgcgtgc acggtttgtc ggtattgctc gtcccgcttg
     2701 tcggcataga ccgcgagggc taccgcttag gacaggcagg cggctattac gatgcgacgc
     2761 ttgcggcgat gaaataccgt ttgcaggcaa aaaccgtggg cgtgggcttt gcctgccagt
     2821 ttgtggacag gctgccgcgc gaaccgcacg atctgctgct ggacggtttt gtgtcggagg
     2881 cggggatatt gtgcttttag cccttcagac ggcatttcgg gcggaaatgc cgtctgaagg
     2941 cggaatattg ggagaaaagg atgtcggaaa ggataatact gccggtcttg tcattgggcg
     3001 gcgttcggct cgaaccgttg gatgtgcatc atgaaacggg tttgcgcgag gcggtttgcg
     3061 atggggaggt ttggaagctg ggggtaacgt ccgcgcccca tccggatagg gtggcagact
     3121 atatcgggac agcattggca acgcgtttgg cgtttgcggt tgtcgatgaa gaggcgggca
     3181 gggtggtcgg gacaacggcg tattatcatt ttgaaccgca gattccgcgt ttggatatcg
     3241 gattcacatg gtatgcggca tcggcacggc ggacacggat aaacacctgt tgcaaaatca
     3301 tgctgttgga ttacgcattt gatgttttgg cttgccgttg cgtgggatgg cgcacggata
     3361 ttctgaacct tgcctcgcaa cgtgccatcg aacggctggg tgcggaaaaa gacggcgtgc
     3421 tgcgtatgca tatgctccgt aaagacggca gtgtgcgcga tacggtcgtg tacagtatgc
     3481 tgagggaaga ttggtgcaaa aacagggaaa tcctgaccgg caggttggca gggtatggcg
     3541 tgcaggtgtg aggcagtatt ttcaaat
//
LOCUS       DAWKQB010000169         3469 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.170, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000169 DAWKQB010000000
VERSION     DAWKQB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.170"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            222..1694
                     /gene="pyk"
                     /locus_tag="WJL14_001901"
     CDS             222..1694
                     /gene="pyk"
                     /locus_tag="WJL14_001901"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ7656928.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2002..3396
                     /locus_tag="WJL14_001902"
     CDS             2002..3396
                     /locus_tag="WJL14_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ7656929.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNERHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANK
                     LALLGYTPSEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKE
                     KTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
BASE COUNT          911 a         1060 c          863 g          635 t
ORIGIN      
        1 taggacagct ccaaaaataa atttagccta agtcataaaa ccgtgtaatt ttgcaccaaa
       61 aaagacggtt tgcaggtaaa atatcaaact attaatttaa atcatatttt tacagaatat
      121 tccgccgcat tcatcaaatg gacatcaaac cggttccaaa tttctgtaat tttgcaacaa
      181 aataccgcaa aacacccgat tgagaccaaa aggactttca tatgaaccag acaagccgcg
      241 atctgacccg catcagccac aacaccaaaa tcgtcgccac ccttgggccg ggcagcaaca
      301 acgtcgaact gttggaagat atgatccgcg tcggcggcct gaacgtcgtc cgcttcaact
      361 tcagccacgg tacgcccgaa ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa
      421 aacgcgccgg acaggaaatc gccatcattg ccgacctgca agggccgaaa atccgcgtgg
      481 gcaaaatcgc cggcggcggc atcgaattga acaaaggcga aacattggta ctcgatgccg
      541 cgctcgaagg cgaaggcacg cgcgaagcgg tcggtttgga ctaccgcgac ctgcccgacg
      601 acgttgccgc aggcgacgta ttgtggctgg acgacggcct gctgaccctg accgtggaat
      661 ccgtcgaagg cagcaggatt atcacaaggg tggaaaacag ccatgtcctg aaaagcaaca
      721 aaggcatcaa caaacgcggc ggcggtctgt ccgcaggcgc gttgaccgaa aaagacttcc
      781 gcgacctgaa aaccgcgatt gccatcggtt gcgactacct cgccatcagc tttgtgaaat
      841 ccgccgaaga tttgcacatc gcgcgcgcca aagtcgaaga agaaatgaag ggcagcacgg
      901 ccgtgcgccc cggtttggtt tccaaaatcg aacgcgtgga agcgattgaa aaccttgatg
      961 aaatcatcct tgccggcgac ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg
     1021 gacacgccgc cgtccccgcc ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc
     1081 gcttcagcat tacggcgacg caaatgatgg aatcgatgat taccaacccc gtgccgaccc
     1141 gcgcggaagt cagcgacgtg gcaaacgcgg tattggacgg taccgatgcg gtgatgtgtt
     1201 ccgccgaaac cgccgtcggc gcgtatccgt ttgaaaccgt cagccaaatg gcaattatct
     1261 gcgcggctgc ggaaaaagag caggattcgc tcaacggcgt tgccgaacag gcggagtatc
     1321 ccgaagcggt cagcaccaac ctggcggttg ccggcggcgc ggtcagcgtg gcgcgcgcgg
     1381 ttcacgccaa agccatcgtc gccctgaccg aaagcggttc gaccgccttt gaaatcagcc
     1441 gccacaacat caccctgccg attttcgcgc tgaccccgag cgtttccgcc caacgccgta
     1501 tggcaatgta ccgcggcgtg cgtccgttga ttttggcaac cagcaccgac cacgacacag
     1561 cgttgaacga agttgaaacc atgctagtgg aacacaacat cctgcattcc ggcgaccaat
     1621 acatcattac cagcggctcg caaatgcgcg aatccggttc gaccaatacg ctggaagtgc
     1681 tgcgcgtcaa ataatccgcc ctgagtggaa aatgccgtct gaagccgatg cccgaagctt
     1741 cagacggcat tttttgcggc ggcgcggcgg ttcgggcaaa ctgaaaaaat tgtgcaaatc
     1801 cggtaacatc ggataaaatc gagtacctat actaaagcga aacaaggcaa ttccgactgc
     1861 cttttttata gtggattaaa tttaaaccag tacggcgttg ccttgccgta ctatctgtac
     1921 tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatattt atccaccgtc
     1981 cgccttttta cggaaaccga aatgacccct tccgcactga aaaaaaccgt cctactgctc
     2041 ggcactgcct ttgccgccgc atccgcacaa gcctccggct accacttcgg cacacagtcg
     2101 gtcaacgcgc aaagcacggc aaatgccgcc gccgcagaag ccgccgacgc atcgaccatc
     2161 ttctacaacc ctgccggcct gaccaaactc gacagcagcc agatttccgt caacgccaac
     2221 atcgtgctgc ccagcattca ttatgaggcg gattccgcca ccgactttac cgggcttccc
     2281 gtccaaggtt cgaaaagcgg caaaatcacc aaaaccacgg tcgcgcccca catctacggc
     2341 gcatacaaag tcaacgacaa tctgaccgta ggcttgggcg tgtacgtccc cttcggttct
     2401 gccaccgaat acgaaaaaga ttccgtgttg cgccacaaca tcaacaaact cggtctgacc
     2461 agcatcgccg tcgaacctgt cgccgcgtgg aaactcaacg aacgccattc cttcggcgca
     2521 ggcatcatcg cccaacatac ttccgccgag ctgcgcaaat atgccgactg ggggattatg
     2581 gaaaaagcga aagcactaaa agaaacaccc cccaatccaa ctaaagccgc ccaaatcaaa
     2641 gccgacggac acgccgatgt caaaggcagc gattggggct tcggctacca actggcgtgg
     2701 atgtgggaca tcaacgaccg tgcgcgcgtg ggcgtgaact accgttccaa agtctcacac
     2761 acgctcaaag gcgatgccga atgggcggca gacgacgcaa tggcgaaaca gttatgggat
     2821 gcaaacaaac tcgcactgct cggctacacg ccaagcgaaa aagcccgcgt taaaatcgtt
     2881 acgcccgagt ctttgtccgt acacggtatg tacaaagtgt ccgacaaagc cgacctgttc
     2941 ggcgacgtaa cttggacgcg ccacagccgc ttcgataagg cggaactggt ttttgaaaaa
     3001 gaaaaaacca tcgtcaacgg caaatccgac cgcaccacca tcacccccaa ctggcgcaac
     3061 acctacaaag tcggcttcgg cggttcttat caaatcagcg aaccgctgca actgcgcgcc
     3121 ggcatcgctt ttgacaaatc gcccgtccgc aacgccgact accgcatgaa cagcctgccc
     3181 gacggcaacc gcatctggtt ctccgccggc atgaaatacc atatcggcaa aaaccacgtc
     3241 gtcgatgccg cctacaccca catccacatc aacgacacca gctaccgcac ggcgaaggca
     3301 agcggcaacg atgtggacag caaaggcgcg tcttccgcac gtttcaaaaa ccacgccgac
     3361 atcatcggcc tgcaatacac ctacaaattc aaataaacgg cccgccgttt gattgtagaa
     3421 atgccgtctg aaacagattt ccgtttcaga cggcattgtt ttaggcgcg
//
LOCUS       DAWKQB010000170         3445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.171, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000170 DAWKQB010000000
VERSION     DAWKQB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.171"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            43..720
                     /locus_tag="WJL14_001903"
     CDS             43..720
                     /locus_tag="WJL14_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ7656930.1"
                     /translation="MCTRKQQGFTLTELLIVMAIAAIMAMIALPNMSQWIASRRIASH
                     AERVANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNKCDSGKKGQGMLAFGDKNGNK
                     IYDNDTADVLLRSVVLNDDINNKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSKFVYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            744..1355
                     /gene="pilV"
                     /locus_tag="WJL14_001904"
     CDS             744..1355
                     /gene="pilV"
                     /locus_tag="WJL14_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ7656931.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMRNHTLSAVGGDFAIDAI
                     KTKAQLAEEQLKRFSYELKNALPDAAAIHYAVCKDSLGAAPTLSAGSAFSQNCDGSAN
                     GDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            1352..2341
                     /locus_tag="WJL14_001905"
     CDS             1352..2341
                     /locus_tag="WJL14_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ7656932.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIGSDVVSNVVQKNPL
                     FSLKKRNSTNKLIPITESSNIGYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIA
                     KPGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDG
                     QWGNPQLLVKKINKMDIRYIYLLVENPGIPPTNSPRYIYGCPEDDDAGKEETFKYTDK
                     FDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            2320..2916
                     /locus_tag="WJL14_001906"
     CDS             2320..2916
                     /locus_tag="WJL14_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ7656933.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCAKGLCTAVNVRT
                     NNTGNEEAFDNIVVQGNPTVEAVKRSCLAKSTGLCIDNKGMEYKKGTRSVSKPPRYII
                     EYLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            2921..3394
                     /locus_tag="WJL14_001907"
     CDS             2921..3394
                     /locus_tag="WJL14_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ7656934.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDKASAEALSETLSADAGCEAFSNRKK"
BASE COUNT          982 a          692 c          862 g          909 t
ORIGIN      
        1 aatttaatcc acgatacatt accagttaac gttctattgc ttatgtgtac acgaaaacaa
       61 caaggtttca cgctaacaga gctgctcatc gtgatggcca ttgcagccat tatggcgatg
      121 atagccctcc ccaatatgag ccaatggatt gcatcccgcc gcattgccag tcacgcggag
      181 cgggttgcca accttttgcg tttctccagg ggcgaagccg tccggctcaa tctccctgtc
      241 tatatctgtc ctgttcaagt taaaaaagac ggtgcgccca acaataaatg tgactccggc
      301 aagaaggggc agggaatgtt ggcttttggc gataagaatg gcaataagat atatgacaat
      361 gatacggcgg atgttcttct ccgcagtgtg gtattgaatg atgatatcaa taataagcgg
      421 attaattatg ccttcaacca tatcgctttc ggtcagactc agccgaccgc tgaccgtgta
      481 gtttggacat tcaatcaaaa cgggacgttc ggttattcta ccaatcagga tctcacgaat
      541 acttccaaat ttgtttactc cgacggttat atccaaatcg tgctgacaga tgcgagagca
      601 gtttcagatg ccgataagaa attccgttcg gcggtggttt tgattgacag cagcggcagg
      661 gtcgaagttt gccctagggg tgatcggcgt actcggcgta ctatgtgcca gtataaataa
      721 cagtttcagt tttaaaaatg aatatgaaga ataatgattg cttccgcctg aaagattccc
      781 agtccggtat ggcgctgata gaagtcttgg ttgctatgct cgttctgacc atcggtattt
      841 tggcgttact gtctgtgcag ttgcggacag tcgcttccgt cagggaggcg gagacacaaa
      901 ccatcgtcag ccaaatcacg caaaacctga tggaaggaat gttgatgaat ccgaccattg
      961 attcggacag caacaagaaa aactataatc tttacatgag aaaccataca ctatcagctg
     1021 tgggtggcga ttttgcgatt gatgccataa aaactaaggc gcagttggca gaggaacaat
     1081 tgaagagatt tagttatgag ctgaaaaatg ccttgccgga tgcggcagcc atccattacg
     1141 ccgtctgcaa ggattcgttg ggtgctgcgc cgacattgtc cgccggcagt gctttttctc
     1201 aaaattgcga tggtagtgca aatggggata ctttgattaa agtattgtgg gtaaatgatt
     1261 cggcagggga ttcggatatc gcccgtacga atcttgagac gaacggcaac aatatcgtat
     1321 atacctatca ggcaagggtc ggaggtcggg aatgagacgt aaaatgctaa acgtaccaaa
     1381 aggcagttat gatggtatga agggttttac cattattgaa tttttggttg cgggcatgct
     1441 cagtatgatt gtcctgatgg cggtcggatc gagttacttc acgtcccgga aattaaatga
     1501 tgcggcaaac gagcgtcttg ctattcaaca ggatttgcgg aatgcggcaa cattaattgt
     1561 ccgcgatgca agaatggcgg ggagcttcgg ttgtttcaat atgtctgagc atatcggaag
     1621 tgatgttgtt tccaatgtgg tgcaaaaaaa ccctcttttt tccttaaaaa aaaggaacag
     1681 tacaaataaa cttatcccca taacggaatc ttcaaatatc ggatatccgg gttttaccca
     1741 gcgccttaac gcattgattt tccaatacgg aatcgatgat gttaatgcaa gcgccgatac
     1801 taccgtcgtc agcagctgtg ccaaaatagc aaaaccgggt aagaaaatat ctaccttgca
     1861 agaagcaaag agtgcattac agattactaa tgatgataaa caaaatggaa atatcacccg
     1921 tcaaaggcat gtggtcaatg cctatgcggt cggcaggatt gccggtgagg aaggtttgtt
     1981 ccgcttccaa ttgaatgatg atgggcagtg gggcaatcct cagttgttgg ttaaaaagat
     2041 taataaaatg gatatacggt atatctatct tctcgtggaa aatcctggta taccgcctac
     2101 caattctccc cggtatattt acggttgccc tgaagatgac gatgccggca aagaggaaac
     2161 attcaaatat acggataaat tcgacagctc cacaaatgct gttacgcccg ccggggtgga
     2221 ggttttattg agtagcggta ctgataccaa gattgccgct tcttcagaca atcatattta
     2281 tgcttaccgt atcgatgcga caatacgcgg gggaaatgta tgcgcaaaca gaacactttg
     2341 acgggaatcc cgacttctga cggacagagg gggttcgcgc tgtttatcgt actgatggtg
     2401 atgatcgtcg tggctttttt ggttgtaact gccgcgcagt cttacaatac cgagcagcgg
     2461 atcagtgcca acgaatcaga caggaaattg gctttgtctt tggccgaggc ggctttgcgg
     2521 gaaggcgaac ttcaggtttt ggatttggaa tatgatacgg acagtaaggt tacatttagc
     2581 gaaaactgtg caaaaggtct gtgtaccgca gtgaatgtgc ggacaaataa tactggtaat
     2641 gaagaggctt ttgacaatat cgtggtgcaa ggcaatccca ccgttgaggc ggtgaagcgt
     2701 tcttgccttg caaaatctac aggcctgtgc attgacaata aagggatgga atataagaaa
     2761 ggcacgagaa gcgtcagcaa accgccgcgt tatattatcg aatatttggg cgtgaagaac
     2821 ggagaaaatg tttatcgggt tactgccaag gcttggggta agaatgccaa taccgtggtc
     2881 gtccttcaat cttatgtaag caataatgat gagtaataaa atggaacaaa aagggtttac
     2941 attgattgag atgatgatag tcgtcgcgat actcggcatt atcagcgtca ttgccatacc
     3001 ttcttatcaa agttatattg aaaaaggcta tcagtcccag ctttatacgg agatggtcgg
     3061 tatcaacaat atttccaaac agtttatttt gaaaaatccc ctggacgata atcagaccat
     3121 caagagcaaa ctggaaatat ttgtctcagg ctataagatg aatccgaaaa ttgccgaaaa
     3181 atataatgtt tcggtgcatt ttgtcaataa ggaaaaacca agggcataca gcttggtcgg
     3241 cgttccaaag acggggacgg gttatacttt gtcggtatgg atgaacagcg tgggcgacgg
     3301 atacaaatgc cgtgataagg cttctgccga agccttatcg gagaccttgt ccgcagatgc
     3361 cggctgtgaa gccttctcta atcgtaaaaa ataaggttgt tttgccgatg ccgtctgaaa
     3421 atcaatgttc agacggcatt tttat
//
LOCUS       DAWKQB010000171         3415 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.172, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000171 DAWKQB010000000
VERSION     DAWKQB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.172"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1175
                     /locus_tag="WJL14_001908"
     CDS             <1..1175
                     /locus_tag="WJL14_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEZ7656935.1"
                     /translation="AAFKAEKNAVKYMDWHERFPELEAQLGAPIENYAPPKHWTRKQL
                     RSMGRVSYLCVDAAEQALTDAGLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTG
                     MSRNFSANTYVRMMPHTAAANIGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLT
                     DMMLAGGGEEFFPSEVYVFDSLYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLE
                     ELEHAKRRGAIIYAELVGYGANSDAYHISTPRPDAQGAILAFQTALQHADLAPEDIGW
                     INLHGTGTHHNDSMESRAVAAVFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRK
                     SNPEGKLPPQLWDGQNDPDLPAINLTGSGSRWETEKRIAASSSFAFGGSNCVLLIG"
     gene            1229..1304
                     /locus_tag="WJL14_001909"
     tRNA            1229..1304
                     /locus_tag="WJL14_001909"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Ile,seq:cat)
     gene            1379..2137
                     /locus_tag="WJL14_001910"
     CDS             1379..2137
                     /locus_tag="WJL14_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ7656936.1"
                     /translation="MKKVSVLIVARNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AESLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            2138..3202
                     /locus_tag="WJL14_001911"
     CDS             2138..3202
                     /locus_tag="WJL14_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7656937.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT          911 a          901 c          831 g          772 t
ORIGIN      
        1 aggcagcatt caaagccgaa aaaaacgccg tcaaatatat ggattggcac gagcgtttcc
       61 ccgaattgga agcgcaactg ggtgcgccga ttgaaaatta cgcgccaccg aaacattgga
      121 cgcgcaagca gctcagaagt atggggcgcg tgtcgtattt gtgcgtcgat gcggcggagc
      181 aggctttgac ggatgccggt ttgctcggag acgaaagtat taccgacgga cggatgggcg
      241 ttgcctgcgg ctcttccagc ggcagcacca aagacatcgg cgatgtgggc gaattgttgc
      301 tgaccggcat gtcgcgcaac ttcagtgcca acacctatgt gcgtatgatg ccgcacaccg
      361 ccgccgccaa tatcggcatc tttttcggac tgaaaggacg catcatcccg acatcgagcg
      421 cgtgttcgtc cggcagccaa ggcataggtt atgcctacga agccatcaaa tacggtctga
      481 ccgatatgat gctggcgggc ggaggcgaag aatttttccc gtccgaagtg tatgttttcg
      541 actcgcttta tgccgccagc cgccgcaacg gcgaaccgga aaaaaccccg cgcccatacg
      601 acgcgaaccg cgacgggctg gtcatcggcg aaggcgcggg gattttcgtg ctggaagaat
      661 tggaacacgc caaacggcgc ggtgcgataa tttacgccga actcgtcggc tacggagcca
      721 acagcgatgc ctaccatatt tccacgcccc gccccgacgc gcaaggcgca atccttgcct
      781 ttcagacggc attgcaacac gcagaccttg cgcccgaaga catcggctgg attaatctgc
      841 acggcaccgg gacgcaccac aacgacagta tggaaagccg cgccgttgca gcggttttcg
      901 gcaacaatac gccctgcacg tccaccaagc cgcaaaccgg acacacgctg ggcgcggcgg
      961 gcgcaatcga agccgcgttc gcgtggggca ttgctgaccg gaaaagcaat cccgaaggga
     1021 aacttccgcc ccagctttgg gacgggcaga acgatcccga ccttcccgcc atcaacctga
     1081 ccggcagcgg cagccgctgg gaaaccgaaa aacgcattgc cgccagctcg tcgtttgcct
     1141 tcggaggaag caactgcgtt ttactcatcg gatgaaataa gtttgtcaat cccaccgcta
     1201 tgctatacaa tacgcgccta ctcttgatgg gtctgtagct caggggttag agcaggggac
     1261 tcataatccc ttggtcgtgg gttcgagccc caccggaccc accaattccc aagcccggac
     1321 gtatgtttgg gcttttttgc cgccctgtga aaccaaaatg ctttgagaaa ccttgataat
     1381 gaaaaaagtc agcgtattga ttgttgctag aaatgaagca aaccacattc gggaatgtat
     1441 tgaaagttgc cgtttcgata aagaagttat cgttattgac gaccacagca ccgacaatac
     1501 tgccgaaatt gccgagagtt tgggtgcaaa agtcttcaga cggcatttga acggggattt
     1561 cggagcgcaa aaaacatttg ccatcgaaca ggcaggcgga gaatgggttt tcctgattga
     1621 tgcagacgaa cgctgcacgc cggaactatc tgatgaaatc tcaaaaattg tccaaaccgg
     1681 cgattatgcc gcctattttg tcgaacgccg caaccttttc cccaaccatc ccgccacaca
     1741 cggcgcgatg cgtcccgaca gcgtatgccg tctgatgccg aaaaaagaca gttcggtgca
     1801 aggcaaagta cacgaaaccg tacaaacccc ctaccccaaa cgccgtctga agcattttat
     1861 gtaccattac acgtacgaca actgggaaca atatttcaac aagttcaaca aatatacttc
     1921 catttcagcc gaaaaatacc gagagcaggg aaagcccgtg cgtttcgtta gggacattat
     1981 cctccgcccg atttgggggt ttttcaaaat ttatatcctg aacaaagggt ttcttgatgg
     2041 aaaaatgggt tggattatgt ccgtcaacca cagctattac acgatgatta aatatgtcaa
     2101 actatattat ctgtacaaat ccggcggaaa attttaaatg gaaaaagaat tcaggatatt
     2161 aaatatcgta tcggccaaga tttggggtgg aggcgaacaa tatgtctatg atgtttcaaa
     2221 agcattgggg cttcgggact gcacaatgtt taccgccgtc aataaaaata atgaattgat
     2281 gcacaggcga ttttccgaag tttcttccgt tttcacgaca cgccttcaca cgctcaacgg
     2341 gctgttttcg ctctacgcac ttacccgctt tatccggaaa aaccgcattt cccacctgat
     2401 gatacacacc ggcaaaattg ccgccttatc catacttttg aaaaaactga ccggggtgcg
     2461 cctgatattt gtcaaacata atgttgtcgc caacaaaact gatttttatc accgcctgat
     2521 acagaaaaac accgaccgct ttatttgcgt ttcccgtctg gtttacgatg tgcaaaccgc
     2581 cgacaatccc tttaaagaaa aataccggat tgttcataac ggtatcgata ccggccgttt
     2641 ccctccctct caagaaaaac ccgacagccg tttttttacc gtcgcctacg ccggcaggat
     2701 cagtccggaa aaaggattgg aaaacctgat tgaagcctgt gtgatactgc atcggaaata
     2761 tcctcaaatc aggctcaaat tggcagggga cggacatccg gattatatgt gtcgcctgaa
     2821 gcgggacgta tctgcttcgg gagcagaacc atttgtttct tttgaagggt ttaccgaaaa
     2881 acttgcttcg ttttaccgcc aaagcgatgt cgtggttttg cccagcctcg tcccggaggc
     2941 attcggtttg tcattatgcg aggcgatgta ctgccgaacg gcggtgattt ccaatacttt
     3001 gggcgcgcaa aaggaaatta tcgaacatca tcaatcgggg attctgctgg ataggctgac
     3061 acctgaatct ttggcggacg aaatcgaacg cctcgtctta aaccctgaaa cgaaaaacgc
     3121 actggcaacg gcaggacatc aatgcgtcgc caaccgtttt accatcaacc ataccgccga
     3181 caaattattg gatgcaatat aaactgcttt cagacggcat atgccgtctg aaagcctttg
     3241 atgcaacaaa ccgccaaatt atattcgttc attggaaaga aacaccccga atccatcctt
     3301 aaaaataaga aaatcccaat atcccccgat attacgcagc ctattggcaa agttttgcag
     3361 cgtcttcccc ggcttgtgct gccgcatcaa gtgctttgtt acaatgtata gtaga
//
LOCUS       DAWKQB010000172         3368 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.173, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000172 DAWKQB010000000
VERSION     DAWKQB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.173"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(198..578)
                     /locus_tag="WJL14_001912"
     CDS             complement(198..578)
                     /locus_tag="WJL14_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ7656938.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            1054..3135
                     /locus_tag="WJL14_001913"
     CDS             1054..3135
                     /locus_tag="WJL14_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ7656939.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMIVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHTGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3125..3319
                     /locus_tag="WJL14_001914"
     CDS             3125..3319
                     /locus_tag="WJL14_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ7656940.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
BASE COUNT          736 a          997 c          858 g          777 t
ORIGIN      
        1 cattggataa cagccctgaa gaaatcggag caggtttaaa gttggcattg agccgctgcc
       61 gatagaataa acccggaaca aaacgaaaaa taatccgcca cgcccgaaaa ccgccggaac
      121 cagccgacgg ccaaaacaaa accggtacaa aaaaccgccc gaaaaatccg gacggcggtt
      181 caaacaggct gccccgttta gcgggcgcgg caggaggttt cgaccgaatt gccgtaggca
      241 tcggtaaagc cgaaaaaggc ttcgccgcct ttctgatgcc actcggttcc gtttccgaac
      301 aaaccgtgtt cggcggtata gcgttcgccg gatgcggcaa cgtcggaaga gaggacggca
      361 cgcctgccgt ccaaccgcaa cgcgattctg ccgccgtcca aacggcggac gtgcacagac
      421 aaaccgttct cgcaggaaaa tgcccgaaaa tctgccgtgc cggcttggtt ttgaacgggc
      481 ggcatatgcc ctcgtccgcc gtcatcatac gcctccggca cggcacaggc cgccaaagac
      541 aaaaccggta cggtcagcgc gaaaaacctg atattcataa aagctcccca ataaaaataa
      601 gatatgaaac aaccgccctg attccaagct gcggcaacgc catactataa acggacgcgc
      661 aaacacacaa gcccgataac cggaatttac ccgcgatgaa tcaataatcc ggattgcgcg
      721 ccccttcttt acccgtcttc cggcgccgcc ttttgcctga cgatgccgtc caaacccgcc
      781 tgtccgcccg aaggaatgta aattttttcc aaattccaag taaaaaccgc tatcagtgtg
      841 ctaatttgcg ttaaaatcct attcggcgtt taacgttttg tgcgcccgca tccctgcact
      901 gtttgatgcg ggcataaggc acaaatcccg acaagcgcac tgtttcatac ttcgtcaatc
      961 attcagactc cggtttgtgc ccgtgccggc aggcggtcat ccgtttcccg ccgttcaggc
     1021 atattccgac agtgtgagat aaggatttat tcgatgaaat cactcaaaac cttcctcatt
     1081 tggggcatag tggtactggt cggcttagca tcctttacca ctctggctct cagccgaggc
     1141 gaacaggtca gcgcggtatg gatggttacc gccgccatat ccgtttactg catcgcctac
     1201 cgtttttaca gcctctacat cgccaaccgc gtaatgcggc tcgatcctaa ccgcctgact
     1261 ccggcagaac gccacaacga cggcttggac tacgttccga cgcacaaggg cgtattgttc
     1321 ggacaccact ttgccgcgat tgccggcgcg ggccccttgg tcggcccggt tttggcggcg
     1381 caaatgggtt acctgcccgg tactttgtgg attatcttcg gcgtggtgtt tgccggcgcg
     1441 gtacaggata tgatagtctt gttcgtctct atgcgccgcg acggtaagtc tttgggcgat
     1501 attgtgaaac aggaactcgg cactgtcccc ggcgtgattg cctccatcgg tattttgatg
     1561 attatggtca tcattatggc ggtgttggcg ttgattgtcg taaaagcact tgttcacagc
     1621 ccttggggta cgtttaccat cgccgccact atgccgattg ccctgtttat gggtatttac
     1681 acgcgctata tccgtccggg caaaatcggc gagatttcca tcgtcggctt tattttgctg
     1741 atgctggcgg taatttacgg cgaagatgtg gcgaaaagtt ccatcggaca ttggttcgac
     1801 cttgacggca tccagctcac ttgggcgatt atgatttacg gctttgtcgc ctccgtattg
     1861 cccgtatggt tgctgctcac tccgcgcgac tatctctcca ccttcctgaa aatcggtacg
     1921 attgcggcct tggctttggg catcgtcatc gtcaatccca ctttgcaaat gcctgccgta
     1981 acccacttta tcgacggttc gggtccggta ttctcaggcg cattgttccc gttcctcttc
     2041 atcaccatcg cctgcggtgc ggtttcgggc ttccacgcgc tgatttcctc cggcaccacg
     2101 ccgaaaatgc tggaaaacga aacccacgtc cgtatgattg gttacggtgg tatgttgatg
     2161 gaaagtttcg tagccattat ggcgcttgcc gccgccgcat cgcttgatcc cggcgtgtac
     2221 ttcgccatga acagccctgc cgccctgatc ggtacggatg ccaacaccgc cgccgaagtg
     2281 attaccacca agctgcaatt ccctgtcgat gcggcaaccc tgttgcatac cgctaaggaa
     2341 gtgggcgaaa acaccatcct ttcccgtgca ggcggtgcgc caaccctcgc agtcggtatg
     2401 gcgcacatta tgagccgcct gattccgggc gaggcgatga tggcgttctg gtatcacttc
     2461 gccctattgt ttgaagcctt gttcatcctg accgccgtcg atgccggtac gcgcgtcgca
     2521 cgttttatga ttcaagactt gggcagcatc ttctacaaac ctttcggcaa caccgactcc
     2581 atccccgcca acctgattgc gaccttcttc gccgtggcat tgtggggcta ctttctctac
     2641 accggcgtaa ccgacccgct gggcggcatc aactcgctct ggcctttgtt cggcatcgcc
     2701 aaccaaatgc tggcgggcgt ggccttgatt atgtgtgccg tggtgctgat taagatgaaa
     2761 cgcgaccgtt atgtttgggt ggtactcgtt cccgccgtcg gcgtactgtt cgtaacctgc
     2821 tacgccggcc tgcaaaaact gttccacagc gatccgcgca tcagcttcct tgcccacacc
     2881 ggcaaataca gcgacgcatt ggctaaaaac gaagtccttg cgcctgccaa agacatcggc
     2941 gaaatggcgc aaatcatctt caacgacaag attaatgccg gtctgaccat cctcttcttg
     3001 tcggttgtcg tgattgtcgc cgcgtacggt ttgcgtaccg ccctcaaagc acgcaaagtc
     3061 ggctggccga ccgccaaaga aatcccggcg gtgtaccgcg acggcaaaca gccggaggca
     3121 caaagtgaag cataagctcg cgtcttggtg gaaaaccatc aagctgacgg caaacttgat
     3181 ggcgggcgtg cccgattatg aaaactacgt tgcacagcag cgcaaacata atcccaacgc
     3241 ccccgtgatg accaagctgc agtttcaaga ctattgccgc aaacgccgct gcggcgcaaa
     3301 cggcggacgc tgctgttaag cctgcttgaa acaaattccg tctgaacgcc gcttcagacg
     3361 gaattttt
//
LOCUS       DAWKQB010000173         3345 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.174, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000173 DAWKQB010000000
VERSION     DAWKQB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.174"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(59..2191)
                     /locus_tag="WJL14_001915"
     CDS             complement(59..2191)
                     /locus_tag="WJL14_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ7656941.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPVKRLAMATLPALVWCDDGNHFILAKTDGGGEHAQYLIQ
                     DLTTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
     gene            complement(2956..3258)
                     /locus_tag="WJL14_001916"
                     /pseudo
     CDS             complement(2956..3258)
                     /locus_tag="WJL14_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670935.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          949 a          901 c          747 g          748 t
ORIGIN      
        1 cggtctgaaa acgcagaacg ttacgaaaaa agcagcctac acgcccatcc cccgccttct
       61 acccgttctg taaatcatac agatagcggt aatatccgtt cggcttcgcc agcaattcct
      121 gctgtgttcc cgcttccaca atcctgcctt tatccatggc aatgatccgg tgtgccgttt
      181 taacggtgga cagacggtgg gcgataatca gcaccgtccg gttggcgcaa atggcctgca
      241 tgttctgcat aatcgctcgt tcactttcat aatccagcgc gctggtggct tcgtcaaaaa
      301 tcagaatgcg cggattggtg attaacgcgc gggcaatcgc aatacgctgc cgctgtccgc
      361 ccgacaagcc ggccccttgt tcgcccacca cggtgccgta gccttccggc agctccataa
      421 taaactcgtg tgcgcccgcc agtttggctg cttcgataat gcgttccagc ggcatacccg
      481 tatccgtcag cgcgatattg tcgcgtatgc tgcggttgag cagcacattc tcctgcaaga
      541 ccacgccgac ctgccgccgc agccaggcag gagcggccaa agccaaatcg ttgccgtcca
      601 ccaacacccg tccctgctcc ggtacataca gacgctgcac caatttggtg agtgtggatt
      661 tgcccgaccc cgaacgtccc acaatcccca gcacttcccc cgcccgaatc cgcaggttca
      721 aatcctgcaa aatcagcctg ccgtccgcct tatagcggaa atcgacatgt tcgaacgtaa
      781 tctccccccg gatatcgggc aaagccaaat gcgaagacgc attctcggtc ggcgcattca
      841 gaatatcccc caaacgcgcc accgaaatcc ccacctgctg gaaatcctgc cacaactgcg
      901 ccaaacggat aacaggcgcc gccacctgtc ccgagagcat attaaacgca atcagctgcc
      961 ccaccgtcag cttgccctca attaccagcc gtgcgccaat ccacaacgtc gccaccgtca
     1021 ccagcttctg aatcagctgc accccctgct ggccgaccac cgccaacttc gttacccgaa
     1081 atcccgaagc cacataagcc gccaactgat tgtcccaacg ctgcgtcatc tgcggctcca
     1141 ccgccatcgc ctttaccgta cccaccgcag tgatgctttc tactaaaaac gactggttgt
     1201 ctgcattgcg cgcgaactta tcgttcagac gcgtccgcag tatcggactg ataaatgccg
     1261 accaaaacgc ataggcaggc aacgaagcca ataccaccca agtcagagtg gagctgtaat
     1321 accacatcac cgccagaaag ataaacgaaa acgccaaatc caacaccgaa gtcagcgcct
     1381 gaccggtcaa gaaattgcga atctgctcca attcccgcac ccgagccacc gtatcaccca
     1441 ctcgtctgtg ctcgaaatag gataaaggca gggaaagcag atgccggaac aaacgcgcgc
     1501 ccaattccac atcaatacgt gaagtcgtat gtgcaaacag atacgtccgc aaaccgccca
     1561 acacaatctc aaacagcgac accaccaaca aagccaccga caccacatcc aaagtagaga
     1621 atccccgatg taccagcacc ttgtccatca ccacttggaa aaacagaggc gtaatcagcg
     1681 caaacagctg caacaccacc gacaccacca atacttcaaa aaacaaccgg cggtatttga
     1741 ttaccgccgg aataaaccag gtaaagtcaa actttgccaa actgcccaat accgaagcgc
     1801 gggaagcaac caatatcagt ttgcccgaat atctgttaga aaattcggca aaagacaata
     1861 ccgcagactt attcgtagtt aaatcctgta ttagatattg ggcatgctca cccccaccgt
     1921 ctgttttagc caaaataaaa tggttgccgt catcacacca taccaatgca ggtaaggtcg
     1981 ccatagccaa acgtttgaca ggctggcgga ccacctttgc cttcaatccc aaagatttgg
     2041 cggctaacag ccattgcgtt tcatttaaat cgctctgtgc ggaagtacaa aattcatgct
     2101 gtatatcggc aggattggcg gcaatgccgt ggtaatgggc gaggatgatg agggcggaaa
     2161 gggcggggag cggtgcggat acgatagaca taaataaaat atagttagat tggatgtgga
     2221 taacggctgg ctggaaaagg aatatattaa gtagaaagaa atatataaat aaaacagcag
     2281 aacgcattgt aaggatatat atgggaattg taaagagaaa gtatggaaaa gttctcgttt
     2341 caggaaggta aaacggctta ggaatcgagt tagatgagga tgcctcgcac ctctcgtgcc
     2401 tcctgcatac cgttaaggca cagggttaag gtgcaggctg ctccgaactc tgttgcggtc
     2461 gggtaatgtt attttttgtg tttcaggcag cctgaaatat ctgtatattt ttgttttaaa
     2521 tagattttaa agattgataa ctgttcttga cgatttttca agaaaggagt aaatttcaag
     2581 aaaggagtaa agtgacttat tatcaatgac aagcaacgcg cgaagtgaca aggaaaacta
     2641 tctacttaaa ttctaaggag gcttcgaata tcataaacca atcagaaaca tagagataaa
     2701 aattatgtac aaatataatc ctcttataca atttattgca cagttgatta tgtcttatgg
     2761 agcaagcgta gggtgggcac ttgctgcccc acgcgtttca tatttcaagg cagcctgaaa
     2821 ccgtgtgggc ataaatgcct accctacatc ccaaaaaaca agcgcagcct gcgtgtgtag
     2881 ggtgcgaact ttcggcaggt agacacgcag ttttatattt tcaagctgag ggatgcttaa
     2941 gaaaagtaca aaacattaaa aaataagggg ctgtactaga ttagccctaa atccacacca
     3001 atcccgcaag atttttagct gtcgggacgg tgtgccgaag ttaaatcgaa attcgcattc
     3061 tttcaagaac agcgggaaag atttgcgatc aattccgttc tatttgcgca agacgcgttt
     3121 tgcctgattc caaaagttct caatgccgtt tatgtggttc tgacggtcag caaattcctt
     3181 ggaatggttg atgcggtaat ggataaaacc gcttacgtcc aacttgtcgt aactgctcag
     3241 gctgtcggta taaacaatgc tgtccggcat gattttctgt ttgataacag gcattaaagt
     3301 atcggacttg gcattatcta cgacaacggt atagacccgt ccgtt
//
LOCUS       DAWKQB010000174         3276 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.175, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000174 DAWKQB010000000
VERSION     DAWKQB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.175"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(698..1282)
                     /gene="rnhB"
                     /locus_tag="WJL14_001917"
     CDS             complement(698..1282)
                     /gene="rnhB"
                     /locus_tag="WJL14_001917"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ7656942.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(1351..3246)
                     /gene="mnmG"
                     /locus_tag="WJL14_001918"
     CDS             complement(1351..3246)
                     /gene="mnmG"
                     /locus_tag="WJL14_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ7656943.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTYEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIEGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVRGQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAVEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVIEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          584 a          787 c         1002 g          903 t
ORIGIN      
        1 cattcccgca aaaacaaaaa aaatcaaaaa cacaaaactg aaatatcgtc attcccgcgc
       61 aggcgggaat ctaggtctgt cggtacggaa acttatcggg aaaaacggtt tttccaaacc
      121 tgagactccg gattcctgtt ttcgcgggaa tccggttttt tgagtttcag tcatttttga
      181 taaattcttg cagctttgag tttctagatt cccgcttttg cgggaatgac gcggaaaagt
      241 tgctgtgatt tcggataaat tttcgtcacg cttaatttct gttttatccg ataaatgcct
      301 gcaatctaaa atttcgtcat tcccgcgaag gcgggaatct aggtctgtcg gtacggaaac
      361 ttatcgggta aaacggtttt gccagccctg agactccgga ttcctgtttt cgtaggaatc
      421 cggttttttg agcttcagtc atttttgata aattcttgca gctttgagtt tctagattcc
      481 cgcttttgcg ggaatggcgg tttggaagtt acctgaaatt caaaaaaaaa aaacggaaac
      541 cggacggatt ggattcccgc ctgcgcggga atgacggatt ttaggttttt ttttgatttt
      601 ctatttttcg cgggaatgac ggtttgggtt ctttctcttt ggagttgcga tgccggaaat
      661 gccgtctgaa ggcttcagac ggcatttttg tgccggttta aaacaaggcc tgctgcgcga
      721 gcaggtttct gacgggggcg aagtcgcggc ggtgttcggg cagcacgccg tatttttcga
      781 gggcttccaa atgctgcttc gtgccgtaac ctttgtgttt gtcgaaaccg tattggggat
      841 ggcgttgcgc cagtgcgtac atttccgcat cgcgggcggt ctttgccaaa acggatgcgg
      901 cggatatttc gatgattttg ctgtcgcctt tgacgacggc ttcggcaggg atgttcaaat
      961 gttcgggaat gcggttgccg tcgatgaata ttttttcggg acgcacagcc aaaccgtcaa
     1021 cggcgcgttt catcgcgagc atggtggcgt gcaggatgtt gaggctggcg atttcttcgg
     1081 gcccggcggc ggcgacgtgc cacgcaaccg cctgattttt tatcatttcg gcaagcgcgt
     1141 cgcgtttttt ctcgctgagt tttttggagt cggtcagtcc gggcaggtcg aatgtttccg
     1201 gaaggatgac ggcggcggca aacacgctgc cgactaaagg tccgcgtcct gcctcgtcca
     1261 cgccggcggt cagtatgtgc atgatgtttc ctgtcgggat ggtgggaatg ccgtctgaac
     1321 ggtgtttcag acggcatcgc gccgatgtgt ttatttcgcg tctttaaatc cgcgcttcaa
     1381 atgcaccatc agcagtgcga cggcggcagg cgttacaccg gaaatgcggc ttgcctgccc
     1441 gacggtttcg ggtttgtgct ggttgagctt ttgctgcact tctgccgaca agcctttgac
     1501 tttgccgtaa tcgatgccgt cgggcagttt taaggtttcg atgtcgcggc ggctgtcgat
     1561 ttcttcgttt tggcggtcga tatagccttg gtatttgact tggatttcga cttgttcgat
     1621 gacttcggcg gagaggtttt cagacggcat cgcgccttcg agcgtcatca gcgcggcgta
     1681 gtcgaggttc gggcggcgta acaaatcgtg caggttggct tctcggctga gtttttgtcc
     1741 gaatacgcgg atttgttcgt cttcggcgag tttttgcggt gtgtaccacg ttgttttcaa
     1801 acgttggatt tcgcgttcga cggcttcgcg tttttcgttg aacatacgcc attgcgcttc
     1861 acccaccaag ccgattttgt agccgtcttc ggtcaggcgc atgtcggcgt tgtcttccct
     1921 gagttgcagg cggtattcgg cgcggctggt gaacattcgg tagggttcgt tcacgccttt
     1981 ggtgatgagg tcgtccacca atacgccgag gtaggcttgt tcgcggcgca gcaggagcgg
     2041 gtcttgtccg cgcacatatt gcacggcgtt cgcgcctgcc aataaacctt gcgcggcggc
     2101 ttcttcgtag ccggtcgtac cgttgatttg cccagcgaaa aacagtccct caatggtttt
     2161 ggtttcgagg ctggctttga ggttgcgcgg gtcgaagtag tcgtattcga tggcgtagcc
     2221 ggggcgcagg atatgggcgt tttccaaacc tttcatactg cggacgagcg cgatttggat
     2281 gtcgaacggc aggctggtgg agataccgtt aggatagtat tcgtacgtag tcaggccttc
     2341 gggttctagg aaaatctggt ggctgtcttt atcggcgaaa cggttgattt tgtcttcgat
     2401 agacggacaa taacgcgggc ccacgccttc gattttgccg gtaaacatcg ggctgcggtc
     2461 gaagcctgag cggatgatgt cgtgggtttg cgtgttggta tgcgtaatcc agcaggacac
     2521 ttggcgcggg tgcatttcgg cgttgccgcg cacggacatg acgggaacgg gtgtgtcgcc
     2581 gggctgttcg gtcagttggg aaaagtcaat cgtgcgtccg tcgatgcgcg gcggcgtgcc
     2641 ggttttcaga cggccttgcg gcagcttcaa ttcgcgcaaa cgtccgccca gcgatttggc
     2701 ggcggggtcg ccggcgcggc cgccttcgta gttttccaaa ccgatatgga ttttgccgga
     2761 caaaaatgtg cctgcggtca gcaccacggc gcgggctttg aattccacgc ccatcgcggt
     2821 gattacgccg ctgatgcgtt cgccgtcgag cgttacgtct tcgacggctt gttggaaaag
     2881 gtcgaggttt tcttggtttt ccaacatttc gcggatggcg gctttgtaca aaatgcggtc
     2941 ggcctgcgcg cgcgtggctc gaactgccgc gcctttgctg gcgttcaggc ggcggaactg
     3001 gataccggat ttgtcggtgg ccaacgccat tgcgccgccg agcgcgtcga gttcgcgtac
     3061 caaatgccct ttgccgatgc cgccgatgga agggttgcat gacatttgtc cgagcgtttc
     3121 gatattgtgt gtgagcaaaa gcgtctgcgc gcccatgcgg gcggcggcga gcgcggcttc
     3181 ggtaccggca tggccgccac cgacgacaat cacgtcgtag gttttggggt aaatcatgtg
     3241 ggtcatgtgt gtattgcctg acggtgtttc agacgg
//
LOCUS       DAWKQB010000175         3258 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.176, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000175 DAWKQB010000000
VERSION     DAWKQB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.176"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WJL14_001919"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WJL14_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ7656944.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLHQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKSRLKGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVLRDRIRNIKESLLFGAE"
     gene            2186..3070
                     /locus_tag="WJL14_001920"
     CDS             2186..3070
                     /locus_tag="WJL14_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656945.1"
                     /translation="MTSIILELQSLAANPNSDVEELVSKTLMVARKLKINELTEWCNN
                     ELQGYSSKNLPKYRIFYGELKAFNPYRGDVPFMLPDELRERVTRVEYTSSISEIRNLL
                     KTKNNNFIRILSSKEKQFLMNLQDTPFPLEPKIIIYPINLMNIQSKVRQLILAWALRL
                     EEEGILGDGIQFTKSKQEKAMSNQFNIQNMQGFIGDIFGGNVQQNIQDGIHIEKENFN
                     KLANFLAKNGIPSAELEDLRNALIEDGQPTDKKFGSKVSAWIGNIITKASTGAIDVSV
                     GTIAGILTNAISKYYGLM"
BASE COUNT          997 a          861 c          675 g          725 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt catccgatac cccgattccc ctttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccggct tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccca gccgcgacct gttcattgaa aaagacagcg
      481 cgatcaacga acatatcgag cagatgcgcc tttccgccac caaaaacctg atgacgcgcg
      541 atgacgtgat tatcgttgcc actgtgtccg ccatttacgg tatcggcgac ccgactgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgacaccat cgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga tttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgca
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 tgcaccaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgagcaac aacgcatcga acagcgcacc cgtttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggtatcga aaactactcc cgtcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacacaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgatt acggcttccg cctaccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ccacccccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgaccct caaatcatca tccgccccgt cgccacccaa gttgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgtactcgtt accaccctta
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttacggctcg
     1621 gattgtttga cgtactcgtc ggcatcaatc tcttgcgcga aggcctcgac atcccagaag
     1681 tctccctcgt cgccatcctc gacgcagaca aagaaggctt cctgcgctcc caccgcagcc
     1741 tgattcaaac cataggccgc gctgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acagcggcaa aagccgtctg aaaggtaaaa
     1981 acaaggttaa agtaggcgaa atccacaacg aagaagatgc gattaaagaa atcgccaaac
     2041 tagaaaaagc catgcaacag gcggctaggg atttgcagtt tgaagaagcg gctgttcttc
     2101 gtgataggat acggaatatt aaagagagtt tgttgtttgg ggcggaataa ttttattatt
     2161 tttatttaac atggagatta attaaatgac ttctattatt ttagaactcc aaagcctagc
     2221 tgccaatcca aatagtgatg tagaagagtt agtgagtaaa acattaatgg ttgcaagaaa
     2281 attaaaaatt aatgaattaa ctgaatggtg taataatgaa ttacaaggtt attcatcaaa
     2341 aaatttacca aaatatagaa tattttatgg agagttaaaa gcttttaacc cttatcgtgg
     2401 cgatgttcca tttatgcttc ccgatgaatt gagagaacga gtaacaagag ttgaatatac
     2461 ctcatcaatt agcgaaataa gaaacttact aaaaacaaaa aataataatt ttatacgaat
     2521 tctatctagt aaagaaaaac agtttttaat gaatttacaa gatactccat ttccacttga
     2581 acctaaaata attatttatc caattaactt aatgaatatt caatcaaaag taagacaact
     2641 aattttagct tgggcattac gactggaaga ggaaggcatt ttaggagacg gtatccaatt
     2701 tacaaaatca aaacaggaga aagcaatgtc aaaccaattc aatattcaga atatgcaagg
     2761 gtttattggg gatatttttg gcggtaatgt tcagcaaaat attcaagacg gtattcatat
     2821 tgaaaaagaa aatttcaata aactggctaa ctttttagca aaaaacggga ttccatctgc
     2881 cgaattagaa gatttacgaa atgcactgat agaagatggt cagcccacag ataaaaaatt
     2941 cggctcaaaa gtaagtgcat ggattggcaa tattattaca aaggcttcta ccggtgcaat
     3001 tgatgtttca gtaggtacta ttgcaggaat attaactaat gcgatttcaa aatattatgg
     3061 cttaatgtaa gcaaatgcaa tatgtgcggc tccaccatac acgccgtccc caaccgccca
     3121 aaaccgcgtg cctgcctgcg gcttaccccc taccacccaa ccaacacagc aagcgtaggt
     3181 tggatacttg tatccgacaa aacctttaac attcccatca ttgcaatcca ttgtagcaat
     3241 gcccaaaatg tcggattc
//
LOCUS       DAWKQB010000176         3254 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.177, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000176 DAWKQB010000000
VERSION     DAWKQB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.177"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..592)
                     /locus_tag="WJL14_001921"
     CDS             complement(<1..592)
                     /locus_tag="WJL14_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ7656946.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKMFSLYREDSLISRLN
                     RDGYLTSPPADFLELLSLCRDIHALTEGAFDPTVQALWKLYADYFAAHPDAETPPPQN
                     SVDEALKRVGFGKVSFDDREIRFAEKGMGLSLNGIAQ"
     gene            complement(601..1395)
                     /locus_tag="WJL14_001922"
     CDS             complement(601..1395)
                     /locus_tag="WJL14_001922"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ7656947.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVLEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFAFKFEDFELEGYDPHPHIKAAVSV"
     gene            1818..3152
                     /gene="gdhA"
                     /locus_tag="WJL14_001923"
     CDS             1818..3152
                     /gene="gdhA"
                     /locus_tag="WJL14_001923"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ7656948.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMSEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          849 a          927 c          784 g          694 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttcatccacg ctgttttgag
      121 gcggcggcgt ttccgcatcg ggatgagcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccttccg tcagcgcgtg aatatcacgg cacaggctca
      241 acagttccaa aaaatccgcc ggcggcgaag tcaaataacc gtcacggttc agacggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca ttttttccaa acgcgccact tccgccaaaa
      361 ccttgttgac caaatccgcc gcacgcctgt cgtccacacc gaacagacgg agctccgcac
      421 cggaacccag tgcgacacct ttccagaaaa acacattttc attgcgtttt tcatctttat
      481 tgcgtttttc atcatcggcg gcaaaaggat tcggcaggaa agaaaccgcc gcgcccgccg
      541 ccgcaacggc ggcaactgtc aaaaaacgcc tgcgcccgaa atgcctgccc ataccgcctc
      601 ctaaaccgac actgccgcct tgatgtgcgg atgcggatcg tagccttcca actcaaaatc
      661 ttcaaatttg aaggcaaaca aatctttgac ttcaggattg attttcatca caggcaaggc
      721 gcgcggttcg cgttccaact gcaatgcggc ctgctcgaaa tggttgcggt acaggtgcgc
      781 gtcgccaaac gtatggacaa actcgcccgc ctccaatccg cacacttgcg ccatcatcat
      841 ggtcaacaat gcgtagctgg caatattaaa cggcacaccg aggaaaatat ccgcgctgcg
      901 ctggtaaagc tggcaggaca gtttgccgtc ggcaacgtaa aactgaaaca gcgcgtggca
      961 gggcggcaag gccatttcat cgaccaaagc cggattccac gccgatacaa tcaggcggcg
     1021 cgaatcgggg tttttcttaa tttgttccag cacattggcg atttggtcga tatgcctgcc
     1081 gtcgggcgcg ggccagttac gccactggta gccgtaaacc gggcctaagt cgccgttttc
     1141 gtccgcccac tcgtcccaaa tggaaacatt gttgtccttt aggtatttga tattggtatc
     1201 gcctttgaga aaccaaagca gctcgtggat aatcgagcgc aaatgcagct ttttggtcgt
     1261 cagcagcgga aaacctttgc ccaagtcaaa acgcatctga taaccgaata cggagcgcgt
     1321 gcccgtaccg gtgcggtctg atttgtccgt accgttgtcg aggacgtggc gcatcaagtc
     1381 caaataggct ttcatagcag tctttcatca aattaaacgg cgcatattgt aacatttccg
     1441 gccaacgccc gagtccggac aaacgcacac gccgccccgc cggacgataa aaacgcagat
     1501 acgggcaggc aggattgttg gcaatttcag tccttttcca cagtaaaacc cggtgggaaa
     1561 acaaaattac cttgattgga atcaaaaaat ctagtttaat tacttagaat aaaatttcaa
     1621 taatattttt atttacgaaa ttaatttatg attattgatt aattatcggc aacaaacatc
     1681 aaacatcgaa aacatggaaa aaataatgtc aacaattttt gccaaatcgg gcttggcatc
     1741 agaaaaaaat aggtttatat tcccacctac aaatttgttt tcccattagt acactatcaa
     1801 ccaaaaggag tatccgaatg actgacctga acaccctgtt tgccaacctc aaacaacgca
     1861 atcccaatca ggagccgttc catcaggcgg ttgaagaagt cttcatgagt ctcgatccgt
     1921 ttttggcaaa aaatccgaaa tacacccagc aaagcctgct ggaacgcatc gtcgaacccg
     1981 aacgcgtcgt gatgttccgc gtaacctggc aggacgataa agggcaagtc caagtcaacc
     2041 ggggctaccg cgtgcaaatg agttccgcca tcggtcctta caaaggcggc ctgcgcttcc
     2101 atccgaccgt cgatttgggc gtattgaaat tcctcgcttt tgaacaagtg ttcaaaaacg
     2161 ccttgaccac cctgcctatg ggcggcggca aaggcggttc cgacttcgac cccaaaggca
     2221 aatccgatgc cgaagtaatg cgcttctgcc aagcctttat gaccgaactc taccgccaca
     2281 tcggcgcgga caccgatgtt ccggccggcg acatcggcgt aggcgggcgc gaaatcggct
     2341 acctgttcgg acaatacaaa aaaatccgca acgagttttc ttccgtcctg accggcaaag
     2401 gtttggaatg gggcggcagc ctcatccgtc ccgaagcgac cggctacggc tgcgtctatt
     2461 tcgcccaagc gatgctgcaa acccgcaacg atagttttga aggcaaacgc gtcctgattt
     2521 ccggttccgg caacgtggcg caatacgccg ccgaaaaagc catccaactg ggcgcgaaag
     2581 tgctgaccgt ttccgactcc aacggcttcg tcctcttccc cgacagcggt atgagcgaag
     2641 cgcaactcgc cgccttgatc gaattgaaag aagtccgccg cgaacgggtt gccacctacg
     2701 ccaaagagca aggtctgcaa tactttgaaa accaaaaacc gtggggcgtc gccgccgaaa
     2761 tcgccctgcc ctgcgcgacc cagaacgaat tggacgaaga agccgccaaa accctgttgg
     2821 caaacggctg ctacgtcgtt gccgaaggtg cgaatatgcc gtcgactttg ggcgcggtcg
     2881 agcaatttat caaagctggc atcctctacg ccccgggcaa agcctccaac gccggcggcg
     2941 tagcaacttc aggtttggaa atgagccaaa acgccatccg cctgtcttgg actcgtgaag
     3001 aagtcgacca acgcctgttc ggcatcatgc aaagcatcca cgaatcctgt ctgaaatacg
     3061 gcaaagtcgg cgacacagta aactacgtca atggtgcgaa cattgccggt ttcgtcaaag
     3121 ttgccgatgc gatgctggcg caaggcttct aagcaaacac cgccgctccg caaacaaaat
     3181 gccgtccgaa ccgcaaatgc tgttcagacg gcatttcatt atccgcccgt tcgaatcggg
     3241 tgatacttat agtg
//
LOCUS       DAWKQB010000177         3214 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.178, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000177 DAWKQB010000000
VERSION     DAWKQB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.178"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..132
                     /locus_tag="WJL14_001924"
     CDS             <1..132
                     /locus_tag="WJL14_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ7656949.1"
                     /translation="LYAGMAGLSGQISLTVPVLLTAQVLWVIIPLVLAAGIFRKRQI"
     gene            129..623
                     /locus_tag="WJL14_001925"
     CDS             129..623
                     /locus_tag="WJL14_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ7656950.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(711..2372)
                     /locus_tag="WJL14_001926"
     CDS             complement(711..2372)
                     /locus_tag="WJL14_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ7656951.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEKNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVMHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSLYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACTPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(2502..3045)
                     /locus_tag="WJL14_001927"
                     /pseudo
     CDS             complement(2502..3045)
                     /locus_tag="WJL14_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
BASE COUNT          724 a          875 c          800 g          815 t
ORIGIN      
        1 ctatatgcgg gtatggcggg tttgagcgga caaatcagcc tgaccgtccc cgttttgctg
       61 accgctcagg ttttatgggt tatcattccg cttgttttgg cagccggaat ttttagaaag
      121 cgacaaatat gaaaaaaacc ctgttggcaa ttgttgccgt ttccgcctta agtgcctgcc
      181 ggcaggcgga agagggaccg ccgcctttac cccggcagat tagcgaccgt tcggtcggac
      241 actattgcag tatgaacctg accgaacaca acggccccaa agcccagatt ttcttgaacg
      301 gcaaacccga tcagcccgtt tggttctcca ccatcaagca gatgttcggc tataccaagc
      361 tgcccgaaga gcctaaaggc atccgcgtga tttacgttac cgatatgggc aatgttaccg
      421 attggacgaa tcccaatgcc gacacggagt ggatggatgc gaaaaaagcc ttttacgtca
      481 tcgacagcgg ctttatcggc ggtatgggtg cggaagacgc gctgccgttc ggcaacaaag
      541 agcaggctga gaaatttgca aaggataaag gcggtaaggt tgtcggtttc gacgatatgc
      601 ctgataccta tattttcaaa taatattgaa aaaaccggcc gaaactttaa aaacgagttt
      661 cggtcggttt ttcttttctt gttcggtata gtgtcggcag gaaagaacct tcacatcccg
      721 ccgtaattcg gcccgctcgc gccttcggga caaatccaag tgatgttttg cgtcgggtct
      781 ttgatgtcgc aggttttgca gtgcacgcag tttgccgcgt tgatttgcag gcgcgggctg
      841 ccgttttctt cgacgatttc atacacaccc gccggacaat agcgcgtttc gggcgaggcg
      901 tattctttgt agttcacgtc tatcatcgtt tgcggattgt tcagcaccaa atggtcgggc
      961 tggttttctt cgtgcgcaag attggcaagg aagacgctgc tcaagcggtc gaaggtcaac
     1021 acgccgtcgg gtttcggata atcaatcggt gtgcacgcgg cagctttttt gagctgctcg
     1081 ttgtctttac cgtgatgttt caaggtccac ggggctttgc ctctgaaaat catctggtcg
     1141 ataccggtat aaagcgagcc gaggtaaacg ccccatttga atgacggacg gacattgcgc
     1201 gcggcgtaaa gctcttgata cagccagctt tgttcaaaac gttgctgata atccgccgcc
     1261 tctttgccgc tgtcgaagtt ttccacttct tcaaggtttt ccaacaaggg gaacacggct
     1321 tcggcggcga gcatggcgga tttcatcgcg gtatgaatgc ctttgatgcg cggcatattg
     1381 aggaaacccg ccgcatcgcc gaccaaaacg ccgcctttga acgagagctt cggcaaactt
     1441 tgcaaaccgc cttcaatcag cgaacgcgcg ccgtaagcga tgcggcggcc gccttcaaag
     1501 gttttgcgga tttcgggatg ggttttgaaa cgttggaact cttcaaacgg cgacagataa
     1561 ggattttgat agtccaaacc gaccacgaag ccgacggcga ctttgttgtc gtcgaaatgg
     1621 taaacaaacg cgccgccgta ggttttgctg tccagcggcc agcctgcgct gtgcatcacc
     1681 aaaccgggct gatgctgttc ggacggcact tcccaaactt ctttaatgcc caagccgtaa
     1741 gtttgcggct ggctgttttg gtcgagtttg aagcgttcga tgatttgctt ggaaagcgaa
     1801 cctcggcaac cttcggcaaa cagggtttgc tgtgcccaaa gctccatgcc gggctggaat
     1861 gaatcggtcg gctcgccgtt tttgcccacg cccatattgc cggtcgcaat gcctttgacc
     1921 gaaccgtctt cgtgatacag cacttcggca gcggcaaagc caggatagat ttccacgccc
     1981 atattttccg cctgctccgc caaccagcgc acgacttcgc ccaagctgac gatgtagttg
     2041 ccgtgattgt cgaaattggg ggtaatgggc agattgaacg cttttttctc cgtcaggaac
     2101 aacaccttgt cctgcgttac cgtgcgcgtc agcggcgcgc ctttttcttt ccagtcggga
     2161 atcaactcat tcagcgcaat cgggtcaatg accgcgcccg ccagcgaatg cgcgcccgcc
     2221 tccgaacctt tctccaccac gcaaacgctg atttcgcgcc cgtttttttc ggcaagctgc
     2281 ttgagtttga tggcggcgga caaacccgac gggcctgcgc cgacaatcac gacatcgtat
     2341 tgcatactgt cgcgggtgat ggattctgtc atggcggttc ctgtgtattt attattgaat
     2401 tgcaaatccg taattataca acgggaacat atagttacca aatacaacaa aggtcgtctg
     2461 aaaaccatat tttcggtttt cagacgacct ttgtcgaaat ttcaataagc acgccaccat
     2521 tttacctgtc cgaccgcaaa ctccgtctga cgtttcggac tgcgtgtgaa aaacgcctta
     2581 tccccgccgg catccctccc tttcggcaca accgccaaaa tcttacctgc caaatttccc
     2641 tcacgggttt gccaagcatc caaaaactgc gccctgctca tcgaaacatg acccagcgac
     2701 gggtcggcaa gcaaaaccgt attgccgtct ataccgcgca ataccgagaa atgatcatcc
     2761 ttgcggtatt tcagatacac gatgacgggg atttgcaact gtgcaagctg ctcgaaagac
     2821 agggcatagc ctttcgcctc aaaacccaaa tcaggcatga tgcgccgcat atcctcaaac
     2881 gacgcgcgca tctgctcctt atccagcttt ttcaacacgt cctcttccgt cagcttttgc
     2941 ccgtgaaaat tgttcaaaag ccgtcgccac cgaagccgcc ccgcaggaaa aatccaaatc
     3001 ctgctttaca atattgaaat cgcgcctttc tttccaactc tgcactttga tttttccata
     3061 agcaacagga ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgccgta
     3121 ctatctgtac tgtctgcggt ttcgtcgcct tgtcctgatt tttgttaatc cactataggt
     3181 ttccgtgcgg acgtgttcag attcccgcct tcgc
//
LOCUS       DAWKQB010000178         3137 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.179, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000178 DAWKQB010000000
VERSION     DAWKQB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.179"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(329..805)
                     /gene="greA"
                     /locus_tag="WJL14_001928"
     CDS             complement(329..805)
                     /gene="greA"
                     /locus_tag="WJL14_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ7656952.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1197..2498
                     /gene="aroA"
                     /locus_tag="WJL14_001929"
     CDS             1197..2498
                     /gene="aroA"
                     /locus_tag="WJL14_001929"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ7656953.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFDVFSSLTETAE"
     gene            complement(2541..3074)
                     /locus_tag="WJL14_001930"
     CDS             complement(2541..3074)
                     /locus_tag="WJL14_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ7656954.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SQIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          658 a          848 c          845 g          786 t
ORIGIN      
        1 ctttcgcggg aatgacggga ttttaggttt ctgattttgg ttttctgttt ttgaggaatg
       61 acgggatgca ggttttctta accctgcgtc ctagattccc gcttttgcgg gaatgacggc
      121 gacagggttg ctgttatagt ggatgaacaa aaaccggtac ggcgttgcct cgccttagct
      181 caaagagaac gattctctaa ggtgctcaag caccaagtga atcgattccg tactatctgt
      241 actgtcttcg gcttcgtcgc cttgtcctga tttttattaa tccactataa tttcctgcgt
      301 gtgtcgggtg tatcgaaatc aagccgaatc aaatatatcg gacttcgata atgtcgtatt
      361 cgcgcacgcc gcccggggct tggacttccg ccgtatcgcc ctcttccttg ccgattaagg
      421 cgcgggcaat cggtgagccg acatagattt tgccctgttt gatatccgct tcgtcttcgc
      481 cgacaatttg gtaggtaacg tgttcttccg tttccaaatc ttccagcgta accgtcgtac
      541 cgaacacgat tttgccttcg gcgtggattt cggtcggatt gatgatgtgg gcaacggaaa
      601 gtttgtgttc cagctcggaa atgcggcctt cgataaagcc ttggcgttct ttggcggctt
      661 catattcggc gttttcggac aaatcgccgt gcgatctggc ttcagcaatc gcttcgatca
      721 cttcggggcg cgccacgctt ttaagctgct gcaattcctg tttcagcaat tccgcaccgc
      781 gtacggtcag ggggattttt tgcatcggtc ttttttctcc atattccggc acaccggttt
      841 gcggcagcaa acataccgca taccgtcttg ttttgtgcat ccggatatta aaataaaaac
      901 acaagccgcc cggaaaatcg gcggcttgtc tgtcgttgaa cagcggctat tctaccaaat
      961 tctatgaaat tggcaatcgt gccgtgccgt ccgcaaacac gctatgtccg caacaaaagc
     1021 cgaaaatatg ccggcaaaaa aattttagaa acaaaaaatt taaaaataat caattttcgg
     1081 cacaaaaaac catattcacg gactttaaaa ccgaaaatgc caaacctgag atttttcaga
     1141 cggcatttgc accagtataa tgcaggctgt ttttatcttt atatttacgt tttgccatga
     1201 ccgaatccct ccgcctccct gccgcctcgc tcaaaccttc cactgtcgcc ctgcccggct
     1261 ccaaaagcat cagcaaccgc accctgctgc ttgccgcctt gtccgacaat gcttgcgaaa
     1321 tccattccct gctcaaatcc gacgataccg accgtatgct cgaagcactc gataaactcg
     1381 gcgttgaaat cgaatatctt gccgaagacc gtctgaaagt gcacggcaca ggcggacgct
     1441 tccccaaccg cactgccgat ttgtttttgg gcaacgcggg cacggcgttc cgcccgctga
     1501 ccgccgcgct tgccgttttg ggcggcgatt atcgcctgca cggcgttgcg cgtatgcacg
     1561 aacgccccat cggcgatttg gccgatgcac tgcggattgc cggcgccgat gtcgaatatc
     1621 tcggcaagga acactatccg ccgcttcata tcggcgaacg ccaagacaac ggcgagcgcg
     1681 tgattccgat taaaggcaat gtatccagcc agtttctgac cgccctttta atggcgttgc
     1741 cgctgaccgg gcaggcgttt gaaatccgta tggtcggcga gttaatttcc aagccctata
     1801 tcgacattac tttaaaactg atggcgcaat tcggcgtaca ggttatcaat gaaggctacc
     1861 gcgtcttcaa aattcccgcc gatgcgcact accacgcgcc cgaacacttg cacgtcgaag
     1921 gcgatgcctc cagcgcgtcc tacttcctcg cagccggttt gattgccgcc acgcccgtcc
     1981 gcgttaccgg tatcggcgca aacagcatac agggcgatgt cgcctttgcc tgcgagctgg
     2041 aaaaaatcgg tgcggacgtg gtttggggcg aaaacttcgt cgaagtctcg cgcccgaaag
     2101 aacgtgccgt ccaagccttt gatttggatg caaaccatat ccccgatgcc gccatgaccc
     2161 tcgccgtcgt cgcgcttgct acagggcaaa cctgcacgct tcgcaacatc ggttcgtggc
     2221 gcgtcaaaga gaccgaccgc attgccgcaa tggcgaacga gttgcgcaaa ctcggtgcaa
     2281 aagtcgccga agaagccgaa gcaattcaca tcaccccgcc cgaaacgccg acacccgacg
     2341 ccgtcatcga cacttacgac gaccaccgca tggcaatgtg tttctcgctg gtttcgctgt
     2401 tggacgtacc cgtcgtcatc aacgatccga aatgcaccca caaaaccttc ccgacttatt
     2461 tcgacgtgtt ctcatcgctg accgaaacag cggaataagg cggcattttg ccgcgattcc
     2521 ggcgcggcgc ggcgggcggc tcattctgta aagaaagtat gtgcgccgag gtagtttttg
     2581 gcgtaaaacg gtgtggagag tttttcggtt ttgatggttt tgccgctgct gggggcatgg
     2641 atgaattcgc cgttgccgat atagagtccg acgtgtgagt agcggtgtgc gccgccggtg
     2701 ttgaagaata cgaggtcgcc ggccttaagg cggctgtcgg ggattttgcg gcttgccgcc
     2761 gccatgtcgc gggcggtgcg cggcagcttg acgttgaggg cgtttttgta aacgaattga
     2821 atcatgccgc tgcaatcgaa gccggttgcg gtgctgctgc cgccccattt gtagggcgtg
     2881 ccgatgagtc cgaggctgtg gagcatgagt tcctgcgagc cttgtgtgcg gtcgatctgg
     2941 ctgatgcgga cggcttggat ttgccggact gtctgtttgg gtttcggttg gcggtgtttg
     3001 ccggaggtcg tgccgcatga ggcgaggagc agtgcgctga gacagaggaa aagggttttg
     3061 tcggggggaa acatggtttt tcctttgcgg gttcggatat gccgtctgaa ggtgtttcag
     3121 acggtatagt ggattaa
//
LOCUS       DAWKQB010000179         3113 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.180, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000179 DAWKQB010000000
VERSION     DAWKQB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.180"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            140..2908
                     /locus_tag="WJL14_001931"
     CDS             140..2908
                     /locus_tag="WJL14_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7656955.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFEQEGGLKFNSNSGKWER
                     DFQKSYWKTKWYQKYDAPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTSTFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYGSDDEFKRAFGENSPTYKKHCNQSCGIYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDILGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDNTLCNGKYGGTSKSVLTNFARGRTF
                     LITMSYKF"
     gene            2938..>3113
                     /locus_tag="WJL14_001932"
     CDS             2938..>3113
                     /locus_tag="WJL14_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656956.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFYSHAGKP
                     VFDFGYFWLFRVIY"
BASE COUNT          881 a          768 c          747 g          717 t
ORIGIN      
        1 gtttttgtta atccactata aatatccaat tgaaatcttc agacggtata tcaaatttac
       61 acttttttaa tgtttatgcc gcctgaaaaa aatgctaata tatttcctaa ttgtctgact
      121 gtttattgtt gaggaaaata tgagatcttc tttccggttg aagccgattt gtttttatct
      181 tatgggtgtt atgctatatc atcatagtta tgccgaagat gcagggcgcg cgggcagcga
      241 ggcgcagata caggttttgg aagatgtgca cgtcaaggcg aagcgcgtac cgaaagacaa
      301 aaaagtgttt accgatgcgc gtgccgtatc gacccgtcag gatatattca aatccagcga
      361 aaacctcgac aacatcgtac gcagcatccc cggtgcgttt acacagcaag ataaaagctc
      421 gggcattgtg tctttgaata ttcgcggcga cagcgggttc gggcgggtca atacgatggt
      481 ggacggcatc acgcagacct tttattcgac ttctaccgat gcgggcaggg caggcggttc
      541 atctcaattc ggtgcatctg tcgacagcaa ttttattgcc ggactggatg tcgtcaaagg
      601 cagcttcagc ggctcggcag gcatcaacag ccttgccggt tcggcgaatc tgcggacttt
      661 aggcgtggat gatgtcgttc agggcaataa tacctacggc ctgctgctaa aaggtctgac
      721 cggcaccaat tcaaccaaag gtaatgcgat ggcggcgata ggtgcgcgca aatggctgga
      781 aagcggagca tctgtcggtg tgctttacgg gcacagcagg cgcagcgtgg cgcaaaatta
      841 ccgcgtgggc ggcggcgggc agcacatcgg aaattttggc gcggaatatc tggaacgacg
      901 caagcaacga tattttgagc aagaaggcgg gttgaaattc aattccaaca gcggaaaatg
      961 ggagcgggat ttccaaaagt cgtactggaa aaccaagtgg tatcaaaaat acgatgcccc
     1021 ccaagaactg caaaaataca tcgaaggtca tgataaaagc tggcgggaaa acctggcgcc
     1081 gcaatacgac atcaccccca tcgatccgtc cagcctgaag cagcagtcgg caggcaacct
     1141 gtttaaattg gaatacgacg gcgtattcaa taaatacacg gcgcaatttc gcgatttaaa
     1201 caccaaaatc ggcagccgca aaatcatcaa ccgcaattat caattcaatt acggtttgtc
     1261 tttgaacccg tataccaacc tcaatctgac cgcagcctac aattcgggca ggcagaaata
     1321 tccgaaaggg tcgaagttta caggctgggg gcttttaaaa gattttgaaa cctacaacaa
     1381 cgcaaaaatc ctcgacctca acaacacctc caccttccgg ctgccccgtg aaaccgagtt
     1441 gcaaaccact ttgggcttca attatttcca caacgaatac ggcaaaaacc gctttcctga
     1501 agaattgggg ctgtttttcg acggtccgga tcaggacaac gggctttatt cctatttggg
     1561 gcggtttaag ggcgataaag ggctgctgcc ccaaaaatca accattgtcc aaccggccgg
     1621 cagccaatat ttcaacacgt tctacttcga tgccgcgctc aaaaaagaca tttaccgctt
     1681 aaactacagc accaataccg tcggctaccg tttcggcggc gaatatacgg gctattacgg
     1741 ctcggatgac gaatttaagc gggcattcgg agaaaactcg ccgacataca agaaacattg
     1801 caaccagagc tgcggaattt atgaacccgt attgaaaaaa tacggcaaaa agcgcgccaa
     1861 caaccattcg gtcagcatta gtgcggactt cggcgattat ttcatgccgt tcgccagcta
     1921 ttcgcgcaca caccgtatgc ccaacatcca agaaatgtat ttttcccaaa tcggcgactc
     1981 cggcgttcac accgccttaa aaccagagcg cgcaaacact tggcaatttg gcttcaatac
     2041 ctataaaaaa ggattgttaa aacaagatga tatattagga ttaaaactgg tcggctaccg
     2101 cagccgcatc gacaactaca tccacaacgt ttacgggaaa tggtgggatt tgaacgggaa
     2161 tattccgagc tgggtcagca gcaccgggct tgcctacacc atccaacacc gcaatttcaa
     2221 agacaaagtg cacaaacacg gttttgagtt ggagctgaat tacgattatg ggcgtttttt
     2281 caccaacctt tcttacgcct atcaaaaaag cacgcaaccg accaacttca gcgatgcgag
     2341 cgaatcgccc aacaatgcgt ccaaagaaga ccaactcaaa caaggttatg ggttgagcag
     2401 ggtttccgcc ctgccgcgag attacggacg tttggaagtc ggtacgcgct ggttgggcaa
     2461 caaactgact ttgggcggcg cgatgcgcta tttcggcaag agcatccgcg cgacggctga
     2521 agaacgctat atcgacggca ccaatggggg aaataccagc aatttccggc aactgggcaa
     2581 gcgttccatc aaacaaaccg aaacccttgc ccgccagcct ttgatttttg atttttacgc
     2641 cgcttacgag ccgaagaaaa accttatttt ccgcgccgaa gtcaaaaatc tgttcgacag
     2701 gcgttatatc gatccgctcg atgcgggcaa tgatgcggca acgcagcgtt attacagttc
     2761 gttcgacccg aaagacaagg acgaagaagt aacgtgtaat gatgataaca cgttatgcaa
     2821 cggcaaatac ggcggcacaa gcaaaagcgt attgaccaat tttgcacgcg gacgcacctt
     2881 tttgataacg atgagctaca agttttaaag gcagcccgcg ttttgtggaa aaccgcaatg
     2941 ccgtctgaaa gcccttcaga cggcatttgt ttccccaaac gcatcatcct gccgcaagcc
     3001 tatgccaatc cgttttatcg catcggcaac tcaaagaaaa atccatttta ttcccacgca
     3061 gggaagccgg tttttgattt cggttatttt tggttgtttc gggtaattta tga
//
LOCUS       DAWKQB010000180         3071 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.181, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000180 DAWKQB010000000
VERSION     DAWKQB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.181"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..2382
                     /locus_tag="WJL14_001933"
     rRNA            <1..2382
                     /locus_tag="WJL14_001933"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2479..2592
                     /gene="rrf"
                     /locus_tag="WJL14_001934"
     rRNA            2479..2592
                     /gene="rrf"
                     /locus_tag="WJL14_001934"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2628..>3071
                     /locus_tag="WJL14_001935"
     CDS             2628..>3071
                     /locus_tag="WJL14_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEZ7656957.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGAPAPKGPAKPV"
BASE COUNT          794 a          675 c          916 g          686 t
ORIGIN      
        1 ctgaaacctg atgcatacaa acagtgggag cgccctagag gtgtgactgc gtaccttttg
       61 tataatgggt caacgactta cattcagtag cgagcttaac cgaatagggg aggcgtaggg
      121 aaaccgagtc ttaatagggc gatgagttgc tgggtgtaga cccgaaaccg agtgatctat
      181 ccatggccag gttgaaggtg ccgtaacagg tactggagga ccgaacccac gcatgttgca
      241 aaatgcgggg atgagctgtg gataggggtg aaaggctaaa caaactcgga gatagctggt
      301 tctccccgaa aactatttag gtagtgcctc gagcaagaca ctgatggggg taaagcactg
      361 ttatggctag ggggttattg caacttacca acccatggca aactcagaat accatcaagt
      421 ggttcctcgg gagacagaca gcgggtgcta acgtccgttg tcaagaggga aacaacccag
      481 accgccagct aaggtcccaa atgatagatt aagtggtaaa cgaagtggga aggcccagac
      541 agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag ctcactggtc
      601 gagtcgtcct gcgcggaaga tgtaacgggg ctcaaatcta taaccgaagc tgcggatgcc
      661 agtttactgg catggtaggg gagcgttctg taggccgatg aaggtgcatt gtaaagtgtg
      721 ctggaggtat cagaagtgcg aatgttgaca tgagtagcga taaagcgggt gaaaagcccg
      781 ctcgccgaaa gcccaaggtt tcctacgcaa cgttcatcgg cgtagggtga gtcggcccct
      841 aaggcgaggc agaaatgcgt agtcgatggg aaacaggtta atattcctgt acttgattca
      901 aatgcgatgt ggggacggag aaggttaggt tggcaagctg ttggaatagc ttgtttaagc
      961 cggtaggtgg aagacttagg caaatccggg tcttcttaac accgagaagt gatgacgagt
     1021 gtctacggac atgaagtaac cgataccacg cttccaggaa aagccactaa gcttcagttt
     1081 gaatcgaacc gtaccgcaaa ccgacacagg tgggcaggat gagaattcta aggcgcttga
     1141 gagaactcag gagaaggaac tcggcaaatt gataccgtaa cttcgggaga aggtatgccc
     1201 tctaaggtta aggacttgct ccgtaagccc tggagggtcg cagagaatag gtggctgcga
     1261 ctgtttatta aaaacacagc actctgctaa cacgaaagtg gacgtatagg gtgtgacgcc
     1321 tgcccggtgc tggaaggtta attgaagatg tgcaagcatc ggatcgaagc cccagtaaac
     1381 ggcggccgta actataacgg tcctaaggta gcgaaattcc ttgtcgggta agttccgacc
     1441 cgcacgaatg gcgtaacgat ggccacactg tctcctcctg agactcagcg aagttgaagt
     1501 ggttgtgaag atgcaatcta cccgctgcta gacggaaaga ccccgtgaac ctttactgta
     1561 gctttgcatt ggactttgaa gtcacttgtg taggataggt gggaggcttt gaagcagaga
     1621 cgccagtctc tgcggagccg tccttgaaat accaccctgg tgtctttgag gttctaaccc
     1681 agacccgtca tccgggtcgg ggaccgtgca tggtaggcag tttgactggg gcggtctcct
     1741 cccaaagcgt aacggaggag ttcgaaggtt acctaggtcc ggtcggaaat cggactgata
     1801 gtgcaatggc aaaaggtagc ttaactgcga gaccgacaag tcgagcaggt gcgaaagcag
     1861 gacatagtga tccggtggtt ctgtatggaa gggccatcgc tcaacggata aaaggtactc
     1921 cggggataac aggctgattc cgcccaagag ttcatatcga cggcggagtt tggcacctcg
     1981 atgtcggctc atcacatcct ggggctgtag tcggtcccaa gggtatggct gttcgccatt
     2041 taaagtggta cgtgagctgg gtttaaaacg tcgtgagaca gtttggtccc tatctgcagt
     2101 gggcgttgga agtttgacgg gggctgctcc tagtacgaga ggaccggagt ggacgaacct
     2161 ctggtgtacc ggttgtaacg ccagttgcat agccgggtag ctaagttcgg aagagataag
     2221 cgctgaaagc atctaagcgc gaaactcgcc tgaagatgag acttcccttg cggtttaacc
     2281 gcactaaaga gtcgttcgag accaggacgt tgataggtgg ggtgtggaag cgcggtaacg
     2341 cgtgaagcta acccatacta attgctcgtg aggcttgact ctatcatttg aagaacttca
     2401 agagataaaa gcttactgac tgattcagtt attaccgaat atattgatta aggctttacc
     2461 gatttgtaac agtttaagtt tggcggccat agcgagttgg tcccacgcct tcccatcccg
     2521 aacaggaccg tgaaacgact cagcgccgat gatagtgtgg ttcttccatg cgaaagtagg
     2581 tcactgccaa acaccctttc agaaaacccc cgattattcg ggggtttttg ctttgcccgg
     2641 aaaaaatgtc ggggatggcg ggacagtatc cgtacggcgt ccggtcgggt ttgcggagga
     2701 acggcttgaa actttgggat attcatttta gaatgactcg ttttatcgtc gcaagatgcg
     2761 gtttattgtt tgcaaccctt aaaggaaaaa ccatgaagaa aatgttcgtg ctgttctgta
     2821 tgctgttctc ctgcgccttc tcccttgcgg cggtaaacat caatgcggct tcgcagcagg
     2881 agttggaggc gctgccgggc ataggccctg cgaaggcgaa ggccattgcg gaataccgtg
     2941 cgcaaaacgg tgcgttcaag tctgtagacg atttgaccaa ggtaaagggc atcggccctg
     3001 cggtgctggc gaagctgaag gatcaggctt cggtcggtgc gcccgcacca aaaggcccag
     3061 ccaaaccagt g
//
LOCUS       DAWKQB010000181         3055 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.182, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000181 DAWKQB010000000
VERSION     DAWKQB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.182"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(72..296)
                     /locus_tag="WJL14_001936"
     CDS             complement(72..296)
                     /locus_tag="WJL14_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEZ7656958.1"
                     /translation="MDGLEGLECRIVELEIQSALQEDVIVGLNAMVAELRQMLDLQQA
                     QLRLLYSKLQDKNSDAQEPYSLRDEIPPHY"
     gene            complement(297..1685)
                     /locus_tag="WJL14_001937"
     CDS             complement(297..1685)
                     /locus_tag="WJL14_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ7656959.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLLVGLLVTMGIGSSFSTLPIIAAIYVPLCISL
                     GFSPLATVSIVGTAGALGDAGSPASDSTLGPTMGLNVDGQHDHIRDSVIPTFIHYNIP
                     LMIAGWIAAMVL"
     gene            complement(1771..2592)
                     /gene="prmC"
                     /locus_tag="WJL14_001938"
     CDS             complement(1771..2592)
                     /gene="prmC"
                     /locus_tag="WJL14_001938"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ7656960.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGAREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAYGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2582..3043)
                     /locus_tag="WJL14_001939"
     CDS             complement(2582..3043)
                     /locus_tag="WJL14_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656961.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAAFWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVASIGTFAGKNKERKAASAADGTKNDV"
BASE COUNT          759 a          938 c          741 g          617 t
ORIGIN      
        1 tatatcttag gtttgcatcg gcggaatatt caaacacagc ttttaggggg aatccggatg
       61 ccagcgacgc atcaataatg cggcgggatt tcgtcgcgca gggaatacgg ctcttgcgcg
      121 tcggaatttt tgtcttgcag cttgctgtac aaaagcctca actgcgcctg ctgcaaatcc
      181 aacatctgcc gtaattccgc caccatcgcg ttcaggccga cgattacgtc ttcctgaagc
      241 gcggattgga tttccagttc gacaatacgg cactccagcc cttcaagccc atccatttac
      301 agcaccatcg cggcaatcca tccggcaatc atcagcggga tgttgtagtg gataaaggtc
      361 gggatgacgg aatcccggat atggtcgtgt tgtccgtcaa cgttcaagcc catggtcggg
      421 cccagcgtcg aatcggacgc gggcgaaccg gcatcgccca acgcacccgc cgtaccgaca
      481 atagaaactg tggcaagcgg cgaaaagccc agactgatgc acaacggtac ataaatcgcg
      541 gcaataatgg gcaaagtgga aaaggacgaa ccgataccca tggttaccaa aagccccacc
      601 agcagcatgg ccaatgccgc catgcctttg ctgttgccga atatcgccat actgctttcc
      661 accagcggct ggatatgccc ggtcgcattc atcacggcgg caaaaccctg cgcggcaatc
      721 ataatgaagc cgaccatcgc catcatcttg ataccttcgc cgaatacgtc gtttgccttg
      781 tcgcggttga tgacccccaa catcataaat acggcgaaac cgagcatcgc gcccaacacc
      841 agcgagtctt catacatcaa ctggatggca aaacatacgg caatggcggc ggcggcggcc
      901 aggctgcggt aggcggacgg ctgcggacgg tttgccgcat cggcgttgcc cgccgtatcg
      961 gcattgttgc tttggtacag gcgcggtttg cggtaatgga caaacgccag caggagtccg
     1021 gccagcattc ccaacgcggg aatcgccatt gccgccatta cgttgatgtt tttcacatca
     1081 agctgcggcg cggcggaatg gatgttgccc aacaggattt cattcaaaaa aatcgcgccg
     1141 aagccgtaag gcaggaacat ataagtcgta accagcccga aagtgatgac gcacgcaatc
     1201 aggcggcggt cgattttcag gcggttgaac accaaaagca gcggcggaac aatcatcggg
     1261 ataaaggcga tgtggatggg gatgatgttc tgactcatca tgcccatcac aaggatgatg
     1321 gaaagcagca gccatttgac cgcgccctcg cccgaacgca cgctgtcggg cataccgccc
     1381 cggttcagct tgcggacgac cgcgccggca agctgctgcg gcaggccgga atgggtaatc
     1441 gccattgcaa acgcgccgag catcgcataa gaaagcgcaa tcttcgcacc gccttccaaa
     1501 cctttgttga acacggggat aatccccgcc tggctgacct gtcccgccgc atcggcaatg
     1561 ttttgcagcg gcatacccgc caccgcgccg ccgacaaacg cgccgaccgt caggctcaat
     1621 accacgtgca cgcgcgacag cgacagcacc agcatcacga ttacggcaac tacgactgca
     1681 ttcattgttt atcgctccaa acctataaat gtttacatat cgaaacacat cataacccaa
     1741 taacgggaaa cccgccaatt ttgcaaacaa ttatttcaaa tgcttcatat acttccccag
     1801 cgtaaccctg tccaaacccg ccaaatccgg cagggtttcc actcctgaaa aaccattctc
     1861 cgccaacacg ccgcgcaccg ccgcgccctg atcgaaaccg tgttccagca ataaaaaacc
     1921 gccttccgcc aaacggtcgg gcgcgccttg cgccaaggta cggatgcagc ttaggccgtc
     1981 tgaaaagtcg gtcagcgcga tttgcggctc aaaccgcaaa tcgccttgcg acaaatgttt
     2041 atcgccgttt tcgatatagg gcgggttgga cacgatgatg tcccatttcc cttcagacgg
     2101 catatcggtg tcgaaccacg aaccgtatgc aaattcgacc cgcgcgccca aatctgccgc
     2161 atttttccgc gccgtttcaa gggcgggcgg gctgatgtcg gatgcgcgta caaacgcatc
     2221 ggggcgttcg agcgcgacgg ttacggcaac cgctccgctg cccgtcccca aatcccacac
     2281 gcgcccgttt tccggcaggc gcgccaatac ggcttcgacc aaatgttcgg tttcggggcg
     2341 cggaatcagc acgctcgggt tgactgtaaa gcgtctgcca taaaattcgc gcgcacctaa
     2401 aatataggca accggctcgc cgttcagacg gcgttgcgcc agcctgtccg cccgctgtcg
     2461 gacttcgtcc ggcatttctt ccccgccccg cgtcaacaac tgcacgcgcg tatattccga
     2521 aacatattgc agcagcattc ttgcttcatt tttaggcagt tttgacaagc ccaaccattt
     2581 atcaaacgtc atttttcgtc ccgtctgccg ccgatgcggc ttttctttcc ttattctttc
     2641 cagcaaacgt accgatggag gcaaccgcaa atgcggcata ccacaaataa aatcctgcac
     2701 cgtagcgcac aatatccgat gtattccctg cttcatcgac gtatacggct ttcacactga
     2761 aagccaccaa cgccaagccc caaaatgccg catggacagg cacgaccttc ttccgcaacg
     2821 ccagcaaaac aatggccgcc aaccaaacat aattcgcata gaccgcacaa tacctgatat
     2881 ccaaagaagc aaatatcgac cccaaaatca aaacggtcaa accctccatg cttccatgat
     2941 tgcccaaata aaatgcaaca ttggataaag acgctatcca cagggcaacc gacaccagca
     3001 acatcactat gggaaaactt gatttccgat tctgttcctg catggtttta tccta
//
LOCUS       DAWKQB010000182         2994 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.183, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000182 DAWKQB010000000
VERSION     DAWKQB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.183"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(229..1218)
                     /gene="pdxA"
                     /locus_tag="WJL14_001940"
     CDS             complement(229..1218)
                     /gene="pdxA"
                     /locus_tag="WJL14_001940"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ7656962.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKDLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1378..2805
                     /locus_tag="WJL14_001941"
     CDS             1378..2805
                     /locus_tag="WJL14_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ7656963.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          605 a          820 c          849 g          720 t
ORIGIN      
        1 aaccgaaacc gaacaagccg gattcccgca acattccgtc attcccgtga aaacgggaat
       61 ctagaacctc aaactttcgg ataatctttg aatattgccg tcgcccgaag gtctggattc
      121 ccgcctgcgc gggaatgacg atttggaaat tacccgaaac ccaaaaacaa ctgaaaccga
      181 acagaccgga ttcccgcctg cgcggaaatg acgggtcttt tatcatcttt aaaggctgcc
      241 gcgcgccatc tcgacggcgg tctccacggc agttatcagg ctgccggaat ccgccctgcc
      301 ggttgccgcc aaatcgagcg cggtgccgtg atcgacggag gtgcggataa agggcaggcc
      361 gagcgtgatg ttcacgccct gtccgaagct gtggtatttc aacacgggca gcccttggtc
      421 gtggtacatc gccaatacgg catccgcgcc ttcgagcata aacggttgga acagcgtatc
      481 tgccggatac gggccggcga ggtttatccc ttcgcggcgc aggttttcca atgcggggat
      541 aatggtgtcg gtttcttcgt gtccgaggtg tccgccctcg ccggcgtggg ggttgagtcc
      601 ggcgaccagg attttgggat ttttgatgcc gaatttgtgt tttaagtcgt gatgcagaat
      661 gcgtgcgacg ctttcaatca gcggttgcgt gatggcggcg gcaacgtctt tcagcggcag
      721 gtgggtcgtt acgagggcga cgcgcaggtc tttgccggca agcatcatca caacctgccc
      781 cgtgccgctt ttttccgcca gatattcggt gtgtccgctg aaaaaacctg tgcttgcgcg
      841 cgcgtcgttg atgatgcctt tgtgcagcgg cgcggtaacg atgccgtcga aaatgccgtc
      901 tgaaatgcct gcgagcgcgg tgtccaaaag ttgcagcaca taggcggcgt tggcgggatt
      961 gagtttgccc gcctcaaccg cttcgacggc agggatgtgc agcacttcca gctcgccgta
     1021 tgccgcgccg cctgattccg gatcgaagtc gcgcaagacg acgcttttgc ccaaggcttc
     1081 ggcgcgcgcg cgcaacaggt gtttgtcgcc caataccgcg cagcggcagg gcaggcgtgc
     1141 aaacgccaag tccaaacaaa tatcggggcc gatgccggca ggctcgccgg aagtaacggc
     1201 aaaaacaggc tgtttcatcg tgtttgctcc aaacaaaatg cgattctaac gccgcagccg
     1261 cgcggcgatg taaatttttc tgattttgtt gacaatctgc tagaatgggc gtttacaaaa
     1321 tttaaaccct gcttgcatac cgccaatatg tgcgggtttc aactttaagg aagcgatatg
     1381 aacgagaact ttaccgaatg gctgcacggc tgggtcggcg ccatcaacga tccgatgtgg
     1441 tcatacttgg tttatatgct tttgggtacg gggcttttct tcaccgtaac cacgggcttt
     1501 gtccaattcc gcctgttcgg gcgcagcatc aaagaaatgc tcggcggccg caaacagggg
     1561 gacgaccctc acggcatcac gccgtttcag gcatttgtaa ccggccttgc cagccgcgtg
     1621 ggcgtgggca atatcgcggg cgtggccatc gccatcaaag tcggcggacc gggcgcggtg
     1681 ttttggatgt gggtaaccgc cttaatcggt atgagttcgg cgtttgtcga atcttcgctg
     1741 gcgcagctct ttaaagtccg cgactacgac aaccaccatt tccggggcgg ccctgcctac
     1801 tacatcactc aagggctggg gcagaaatgg ctgggcgtgt tgttcgccct gagcctgatt
     1861 ttctgtttcg gctttgtgtt tgaagcggtt cagaccaata ccattgccga taccgtcaaa
     1921 gcggcgtggg gttgggagcc tcattatgtc ggcgtcgccc tggtgatttt aaccgcgccg
     1981 attatcttcg gcggcatcag gcgcatatct aaagcggcgg aaatcgtcgt ccccctgatg
     2041 gcggttttgt acctctttat cgcgcttttc atcattttga ccaatattcc gatgattccg
     2101 gacgtgttcg gtcagatttt ttcgggcgcg ttcaaattcg acgcggcagc aggcggctta
     2161 ctcggcggtc tgatttcgca aacgatgatg atgggcatca aacgcggcct gtattccaac
     2221 gaggcgggta tgggttccgc gccgaacgcc gccgccgccg ccgaagtgaa acaccctgtt
     2281 tcgcaaggta tgattcaaat gctgggcgtg tttgtcgata ccatcatcgt ttgttcttgc
     2341 accgccttca tcatcttgat ttaccaacag ccttacggcg atttgagcgg tgcggcgctg
     2401 acgcaggcgg cgattgtcag ccaagtgggg caatggggcg cgggcttcct cgccgtcatc
     2461 ctgtttatgt ttgccttttc caccgttatc ggcaactatg cctatgccga gtccaacgtc
     2521 caattcatca aaagccattg gctgattacc gccgttttcc gtatgctggt tttggcgtgg
     2581 gtctatttcg gcgcggttgc caatgtgcct ttggtctggg atatggcgga tatggcgatg
     2641 ggcattatgg cgtggatcaa ccttgtcgcc atcctgctgc tctcgccctt ggcgtttatg
     2701 ctgctgcgcg attacaccgc caagctgaaa atgggcaaag accccgagtt caaactttcc
     2761 gaacatccgg gcctgaaacg ccgtatcaaa tccgacgttt ggtaaatccc gcccttacca
     2821 gagccgcttc cccgcgaagc ggcttttccc tttccgcaca ctgtaaaaac agggcgaaca
     2881 agcgtacaat cccaaccctt tacttttgaa tccatttcgt ttttcagacg gcatattgaa
     2941 tatagtggtt taacaaaaat caggacaagg cgacgaagcc gcagacagta caag
//
LOCUS       DAWKQB010000183         2951 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.184, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000183 DAWKQB010000000
VERSION     DAWKQB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.184"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            721..2889
                     /locus_tag="WJL14_001942"
     CDS             721..2889
                     /locus_tag="WJL14_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7656964.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTSFGDGDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIKR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFSIPTKEKKNGKEVDK
                     PMEQQKKDRADEATVHAYKLSNPTKTDTGAYIEAIHEINGFTLTGGLRYDRFKVKTHD
                     GKTVSNSSLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKAGVSYSKPRFYGYDTHKDKLLSANPEFGAQVGRTWTASLAYRF
                     QNPNLEIGWRGRYVQKAVGSILVSGQKDRQGNLENVVRKGFGVNDVFANWKPLGKDTL
                     NVNLSVNNVFNKYYYPHSQRWNNTLPGVGRDVRLGVNYKF"
BASE COUNT          789 a          845 c          681 g          636 t
ORIGIN      
        1 ttttttgagt tccagttgtt tctgataaat tcttgcagct ttgagttcct agattcccgc
       61 tttcgcggga atgacgcgga aaggttgctt ttcatcattc ccgcgcaggc ttcgtcattc
      121 ccgcgcaggc gggaatccag tctgttcggt ttcggttttt tccgatagat tcctgccgcg
      181 ttaggggttc tagattcccg ctttcgcggg aatgacgcgg aaaagttgct tttcgtcatt
      241 cccgcgcagg cttcgtcatt cccgcgcagg cctcgtcatt cccgcgcagg cgggaatcta
      301 ggttcgttcg gtttcggttt tttccgatag attcctgccg cgttaggggt tctagattcc
      361 cgctttcgcg ggaaggacgc ggaaaggttg cttttcgtca ttcccgcatt tgttaatcca
      421 ctatatttcc gccgtttttt atagtggact aacaaaaacc agtacagcgt tgcctcgcct
      481 tgccgtacta tctgtactgt ctgcggcttc gtcgccttat cctgattttt gttagtccac
      541 tatacatttc cgacaaaacc tgtcaacaaa aaacaacact tcgcaaataa aaacgataat
      601 cagctttaca caaatccccc ctctcgttaa tataaacaaa aataattatt atttttctta
      661 tcctgccaaa ccttaacggt ttggatttac ttcccttcat acactcaaaa ggacgaacaa
      721 atgaacgccc cgtttttccg cctcagcctg ctctcgctta ccctggcggc aggttttgcc
      781 catgcggcag aaaataatgc caatgtcgca ttggataccg ttaccgtaaa aggcgaccgc
      841 caaggcagca aaatccgtac caacatcgtt acgcttcaac aaaaagacga aagcaccgca
      901 accgatatgc gcgaactctt aaaagaagag ccgtccatcg atttcggcgg cggcaacggc
      961 acgtcccaat tcctgacgct gcgcggcatg ggtcagaact ctgtcgacat caaggtggac
     1021 aacgcctatt ccgacagcca aatcctttac caccaaggca gatttattgt cgatcccgct
     1081 ttggttaaag tcgtttccgt acaaaaaggc gcgggttccg cctctgccgg tatcggcgcg
     1141 accaacggcg cgattatcgc caaaaccgtc gatgcccaag acctgctcaa aggcttggat
     1201 aaaaactggg gcgtgcgcct caacagcggc tttgccagca acgaaggcgt aagctacggc
     1261 gccagcgtat tcggaaaaga gggcaacttc gacggcttgt tctcttacaa ccgcaacgat
     1321 gaaaaagatt acgaagccgg caaaggtttc cgcaatgtca acggcggcaa aaccgtaccg
     1381 tacagcgcgc tggacaaacg cagctacctc gccaaaatcg gaacaagctt cggcgacggc
     1441 gaccaccgca tcgtgttgag ccatatgaaa gaccaacacc ggggcatccg cactgtgcgt
     1501 gaagaattta ccgtcggcag cgaagattca cggataaata ttaaacgcca agcccctgct
     1561 taccgcgaaa ccacacaatc caacaccaat ttggcgtaca cgggtaaaaa cctgggcttt
     1621 gtcgaaaaac tggatgccaa cgcctatgtg ttggaaaaag aacgctattc cgccgatgac
     1681 agcggcaccg gctacgcagg caatgtaaaa ggccccaacc atacccgaat caccactcgg
     1741 ggtatgaatt tcaacttcga cagccgcctt gccgaacaaa ccctgttgaa atacggtatc
     1801 aactaccgcc atcaggaaat caaaccgcaa gcatttttga acggcgaatt ctccatcccg
     1861 acaaaagaaa agaaaaacgg taaagaagtc gataaaccga tggaacaaca aaagaaagac
     1921 cgtgcagatg aagccactgt tcacgcctac aaactttcca acccgaccaa aaccgatacc
     1981 ggcgcgtata tcgaagccat tcacgaaatt aacggcttta ccctgaccgg cgggctgcgt
     2041 tacgaccgct tcaaggtaaa aacccacgac ggcaaaaccg tttcaaacag cagccttaac
     2101 ccgagtttcg gtgtgatttg gcagccgcac gaacactgga gcttcagcgc gagccacaac
     2161 tacgccagcc gcagcccgcg cctgtatgac gcgctgcaaa cccacggcaa acgcggcatt
     2221 atctcgattg ccgacggcac gaaagccgaa cgcgcgcgca ataccgaaat cggcttcaac
     2281 tacaacgacg gcacgtttgc cgcaaacggc agctacttct ggcagaccat caaagacgcg
     2341 cttgccaatc cgcaaaaccg ccacgactct gtcgccgtcc gcgaagccgt caacgcaggc
     2401 tacatcaaaa accacggtta cgaattgggc gcatcctacc gcaccggcgg cctgactgcc
     2461 aaagccggcg taagctacag caaaccgcgc ttttacggtt acgatacgca caaagacaag
     2521 ctgttgagcg cgaaccctga atttggcgca caagtcggtc gcacttggac agcctccctt
     2581 gcctaccgct tccaaaatcc gaatctggaa atcggctggc gcggacgcta tgttcaaaaa
     2641 gccgtgggtt cgatattggt gtcaggtcaa aaagaccgcc aaggcaactt ggaaaacgtt
     2701 gtacgcaaag gtttcggtgt gaacgatgtc ttcgccaact ggaaaccatt gggcaaagac
     2761 acgctcaatg tcaatctttc ggttaacaac gtgttcaaca agtactacta tccgcacagc
     2821 caacgctgga acaataccct gccgggcgtg ggccgcgatg tgcgcttggg cgtgaactat
     2881 aagttctaaa acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga cggcatctgt
     2941 tctgataatt t
//
LOCUS       DAWKQB010000184         2931 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.185, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000184 DAWKQB010000000
VERSION     DAWKQB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.185"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..467)
                     /locus_tag="WJL14_001943"
     rRNA            complement(<1..467)
                     /locus_tag="WJL14_001943"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(874..949)
                     /locus_tag="WJL14_001944"
     tRNA            complement(874..949)
                     /locus_tag="WJL14_001944"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(914..916),aa:Ala,seq:tgc)
     gene            complement(956..1032)
                     /locus_tag="WJL14_001945"
     tRNA            complement(956..1032)
                     /locus_tag="WJL14_001945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(996..998),aa:Ile,seq:gat)
     gene            complement(1134..2674)
                     /locus_tag="WJL14_001946"
     rRNA            complement(1134..2674)
                     /locus_tag="WJL14_001946"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          624 a          833 c          622 g          852 t
ORIGIN      
        1 cacggtactg gttcactatc ggtcgatgat gagtatttag ccttggagga tggtcccccc
       61 atattcagac aggatttcac gtgtcccgcc ctacttttcg tacgcttagt accgctgttg
      121 agatttcgaa tacgggactg tcacccacta tggtcaagct tcccagcttg ttcttctatc
      181 tcgacagtta ttacgtacag gctcctccgc gttcgctcgc cactacttgc ggaatctcgg
      241 ttgatttctt ttcctccggg tacttagatg gttcagttct ccgggttcgc ttctctaagt
      301 ctatgtattc aacttaggat actgcacaga atgcagtggg tttccccatt cggacatcgc
      361 gggatcattg ctttattgcc agctcccccg cgcttttcgc aggcttacac gtccttcgtc
      421 gcctatcatc gccaaggcat ccgcctgatg cacttattca cttgactcta tcatttcaag
      481 aacttctttg acttcgttta cctacccgtt gactagggaa gtaaacttga aattcctact
      541 ttgataaagc ttactgcttt gttgtgtctt aatcctgcct tttgtgtttc aggattaagt
      601 cgatacaatc atcacccaaa tactgtgttt gttttctttt ctcttacgag agatttttta
      661 tcctttgcaa agaacaaaaa atcaaaacaa acgctttgtc tttgtttgtt gatttcggct
      721 ttccaatttg ttaaagatcg atgcgttcga tattgctatc tactgtgcaa atcaaaacga
      781 gctgattatt atatcagcat tttgttcttg gtcaagtgtg atgtcgccct gaatggattc
      841 tgttccattc ttccgttttg atttgtacag tattggtgga ggcaaacggg atcgaaccga
      901 tgaccccctg cttgcaaagc aggtgctcta ccaactgagc tatgcccccc gttcttggtg
      961 ggtctgggag gacttgaacc tccgacccca cgcttatcaa gcgtgtgctc taaccagctg
     1021 agctacaaac ccggattctc ttcttaagcg aatcttgcct tcactcaagc ttcttccgca
     1081 tctttttcag tttaccgata agtgtgaatg cctaaagcct cttctttctc tagaaaggag
     1141 gtgatccagc cgcaggttcc cctacggcta ccttgttacg acttcacccc agtcatgaag
     1201 cataccgtgg taagcgggct ccttgcggtt accctaccta cttctggtat cccccactcc
     1261 catggtgtga cgggcggtgt gtacaagacc cgggaacgta ttcaccgcag tatgctgacc
     1321 tgcgattact agcgattccg acttcatgca ctcgagttgc agagtgcaat ccggactacg
     1381 atcggttttg tgagattggc tccgcctcgc ggcttggcta ccctctgtac cgaccattgt
     1441 atgacgtgtg aagccctggt cataagggcc atgaggactt gacgtcatcc ccaccttcct
     1501 ccggcttgtc accggcagtc tcattagagt gcccaactga atgatggcaa ctaatgacaa
     1561 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacagccat
     1621 gcagcacctg tgttacggct cccgaaggca ctcctccgtc tccggaggat tccgtacatg
     1681 tcaagaccag gtaaggttct tcgcgttgca tcgaattaat ccacatcatc caccgcttgt
     1741 gcgggtcccc gtcaattcct ttgagtttta atcttgcgac cgtactcccc aggcggtcaa
     1801 tttcacgcgt tagctacgct accaagcaat caggttgccc aacagctaat tgacatcgtt
     1861 tagggcgtgg actaccaggg tatctaatcc tgtttgctac ccacgctttc gggcatgaac
     1921 gtcagtgttg tcccaggagg ctgccttcgc catcggtatt cctccacatc tctacgcatt
     1981 tcactgctac acgtggaatt ctacctccct ctgacacact cgagtcaccc agttcagaac
     2041 gcagttcccg ggttgagccc ggggatttca catcctgctt aagtaaccgt ctgcgcccgc
     2101 tttacgccca gtaattccga ttaacgctcg caccctacgt attaccgcgg ctgctggcac
     2161 gtagttagcc ggtgcttatt cttcaggtac cgtcatcagc cgctgatatt agcaacagcc
     2221 ttttcttccc tgacaaaagt cctttacaac ccgaaggcct tcttcagaca cgcggcatgg
     2281 ctggatcagg cttgcgccca ttgtccaaaa ttccccactg ctgcctcccg taggagtctg
     2341 ggccgtgtct cagtcccagt gtggcggatc atcctctcag acccgctact gatcgtcgcc
     2401 ttggtaggcc tttaccccac caactagcta atcagatatc ggccgctcga atagcgcaag
     2461 gcccaaaggt cccctgcttt ctctctcaag acgtatgcgg tattagctga tctttcgatc
     2521 agttatcccc cactactcgg tacgttccga tatgttactc acccgttcgc cactcgccac
     2581 ccgagaagca agcttctctg tgctgccgtc cgacttgcat gtgtaaagca tgccgccagc
     2641 gttcaatctg agccaggatc aaactcttat gttcaatctc taacttttta acttctggtc
     2701 tgcttcaaag aaaccgacag gacaatgttc aaaacattat cttgtctgtc tttcaaacag
     2761 tgtgaggctc aaggcactca cacttatcgg taatctgttt tgttaaagag cgtttgaatt
     2821 ataaagtatt tcttccgctt gtcaagatat ctctcgatat ccccaacatt ctgtgctata
     2881 cttttcagtt cgtccgccgc ttctgcagca gcgaagaacc gaactatacg c
//
LOCUS       DAWKQB010000185         2925 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.186, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000185 DAWKQB010000000
VERSION     DAWKQB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.186"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(110..1420)
                     /locus_tag="WJL14_001947"
     CDS             complement(110..1420)
                     /locus_tag="WJL14_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ7656965.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTVDGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRALQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEVLKAK"
     gene            1732..2154
                     /locus_tag="WJL14_001948"
     CDS             1732..2154
                     /locus_tag="WJL14_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656966.1"
                     /translation="MEWVSLFSDPQKDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADPQLGYTPELSVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVSDGDGVQDVASGKKYRQTIVP"
     gene            2241..2925
                     /locus_tag="WJL14_001949"
                     /pseudo
     CDS             2241..2925
                     /locus_tag="WJL14_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244588.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          800 a          711 c          707 g          707 t
ORIGIN      
        1 ccttgtcttt attggcttta tcgataaaca ggataagacc tgaaaaaagc cgatacgcct
       61 ttttggtgta ccggctttgc catactgttc tgcttcagac ggcatcgctt cattttgcct
      121 ttaatacttc ttcgtccagc gatttcaacc acgccagctt ttcgccgatt ttgatttcca
      181 gtccgcgcgg gacgggttgg tagaaatcgg gttcgtccag gccgtcgggc atatagcttt
      241 cgccggcggc gtaggcgttc ggttcgtcgt gggcgtagcg gtattcgcgt ccgtagccca
      301 attccttcat cagcttggtc ggggcgttgc gcaggtggac gggcacttcg tcgctggcgt
      361 tttctttgac gaagtggcgc atttggttgt atgccttgta gcccgcgttg gatttcgcgg
      421 cggcggcaag atacaatacc gcttgcgcca aagccagttc gccttcgggc gagcctaagc
      481 gttcgaaggt ggcggcggca tcgttggcga tttgcaaggc gcgcgggtcg gcaagcccga
      541 tgtcttccca agcgatacgc acgatgcggc gggcgaggta gcgcgggtcg gtgccgccgt
      601 cgagcatacg gcagaaccaa tacagcgcgg cgttcggatg cgaaccgcgc acggatttgt
      661 gcagggcgga gatttggttg tagaaactct cgccgccttt gtcgaaacgg cggatttgcg
      721 ccccgagact gtcggcgaga aattcggcgg ttaagttttt cagacgacgt gtatcggcgg
      781 cgcgtaaaag ttgttccaac aaattcaaca atctgcgcgc atcaccgtcg acggtattca
      841 cgagtaattt ttgcgcatcc gtttcaatcg taaactcttg gtattcaggc aaagccaata
      901 ccttggcaat cagctttttc aggtcgtctg aagacaaggg ttgcaaaaca tacacctgag
      961 cgcggctcaa cagcgcggga ttgacttcaa acgacggatt ttccgtcgtc gcaccaataa
     1021 aggttagcaa accgctttcg acatgcggca aaaacgcgtc ctgctgcgcc ttgttgaagc
     1081 ggtggacttc atcgacaaac aaaatcgtcg cgcgtccctg ctgcaaagcg atttcggctt
     1141 tatcgattgc ctcgcggatg tccttcacgc cggaaaatac ggcggaaaca ggcaaaaact
     1201 gggcgttgaa actctgcgcc aaaatccgcg ccaacgtcgt cttgcccacg cccggcggcc
     1261 cccacagcaa catagaatgc ggcttgccgc cttctaccgc cacgcgcaaa ggtttacctt
     1321 cgccgatgag gtgttcctgc cccaccacgt cgtcaagcgt atgcggacgc aatcgttcgg
     1381 caagcggcgc gtcgggttct cgggcaaaca aatcggtcat aacggctccg tcaacaggtt
     1441 ttcaaacaat atgattatac ggcagggaac ggcggcgtgc cgcatacgga ttccgcccct
     1501 ccgtttgcct taagccgata ttaggcgcat actggaaaag acgagagact tcacacaata
     1561 tatccggcac ggagaccgat tccgcaccgg catgacaata cccaaatcag cgtttcaatt
     1621 aaacattaag gagactaaaa tagaaaattt gctttatcta ccattgcttt gttgattcaa
     1681 tcggcattat gttttgaggc ggaagcccat gaatatacta atattcaaga gatggaatgg
     1741 gtgtctttat tttctgatcc gcaaaaagac gatgatagtc ttataaccct taaagatgaa
     1801 aaaatcactg taaaaaacta tattgtgcct tggtggaaaa aaggtgaaaa ctttagaaaa
     1861 ttagaactta ttaccactgg taaaaatgat aatggaaatt taacggtaaa tggaatttac
     1921 ggcatccaat cttttgcaga cccgcaatta ggatatacgc ctgaattatc ggtgaaaagc
     1981 aataatgata ttctatttaa aaataatctc caatctgttg acgtgaagcc ccattccgga
     2041 aatatccatt tggatgccaa aggaaaaatt caatttaatt tgaatgtttc cgatggtgat
     2101 ggggtacaag atgtagccag tggaaaaaaa taccgacaaa caatagtgcc ttagtgcggt
     2161 atgtcaacac tatcagcccc cccgatgaaa atgacaaagg cagcatatcc tttcattcaa
     2221 aaacaggcaa tttgttcaat atgaacgtta acgttcataa agacacacac catttcggta
     2281 ttcacaacct caacaatacc gatgttcatc tgactgccga agacagcgat aatgtggttg
     2341 cgattaaagg ggaactcgcc gaccctatgc cgacaggaaa tgttgagagt acaagttttt
     2401 taaggggcat tgaaacggct tttaaatcca ataccgtatt aacggggaat aacaataaaa
     2461 tatccatcgc cactgagggc aagggcttgg gattgggcgt aaatggtctc cagtccaact
     2521 ccccctattc cggacacacg acgggcattt tcatcaataa tcaatcaagc gaccttaccc
     2581 aaccttccct tgatggcatc agcaccatca aactcaaagc cgtcaaggga aataatgaag
     2641 ttgatttaaa tgtaaaaaac cacgcctccg taaaaggcat tatcgcgtcg cattctgcaa
     2701 aagcaacgct tgaagccgaa ggggacaata ttgtccaggt taaaaatccg gacaccaata
     2761 cggctttaat cgatgctttg aaaaaacaat accccaatgc ttcgaaagaa ctatacaggg
     2821 tgggggttca atccacgact ggaacagtac gcatttcgcc aaacggcaaa atcgcattaa
     2881 cggaattgaa aacttttgga attgtacaaa acggcatttg cgtca
//
LOCUS       DAWKQB010000186         2904 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.187, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000186 DAWKQB010000000
VERSION     DAWKQB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.187"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..1443
                     /locus_tag="WJL14_001950"
     CDS             <1..1443
                     /locus_tag="WJL14_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ7656967.1"
                     /translation="INGGSLKDNLGDAALGAIVSTVHGEVASKIKFNLSEDYITHKIA
                     HAIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVA
                     GTVSGVVGGDVNTAANAAKVAIENNLLSQEEYALREKLIKKAKGKGLLSLDWGSLTEQ
                     EARQFIYLIEKDRYSNQLLDRYQKNPSSLNNQEKNILAYFINQTSGGNTAWAASILKT
                     PQSMGNLTIPSKDINNTLSKAYQTLSRYDSFDYKSAVAAQPALYLLNGPLGFSVKAAT
                     VAAGGYNIGQGAKAISNGEYLHGTVQVVNGTLMVAGSVSAQAAISAKPAPVTRYLSND
                     SAPALRQALTAESQRIRMKLPEEYRQIGNLAIAKINVKGLPQRMEAFSSFQKGEHGFI
                     SLPETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGNNRNVSGRIDLFT
                     ELKACQSCSNVILEFRNRYPNIQLNIFTGK"
     gene            1448..1831
                     /locus_tag="WJL14_001951"
     CDS             1448..1831
                     /locus_tag="WJL14_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656968.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            2081..>2904
                     /locus_tag="WJL14_001952"
     CDS             2081..>2904
                     /locus_tag="WJL14_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7656969.1"
                     /translation="MLKTPPMLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGG"
BASE COUNT          965 a          627 c          614 g          698 t
ORIGIN      
        1 atcaacggcg gcagcctcaa agacaacttg ggcgatgccg cactgggtgc gatagtcagt
       61 accgtacacg gagaagtagc gagcaaaatc aaatttaatc tcagcgaaga ctacattacc
      121 cacaagattg cccatgccat agcgggctgt gcggcagcgg cggcgaataa gggtaagtgt
      181 caggatggtg cgatcggtgc ggctgtgggc gagatagtcg gggaggcttt gacaaacggc
      241 aaaaatcctg ccactttgac agctaaagaa cgcgaacaga ttttggcata cagcaaactg
      301 gttgccggta cggtaagcgg tgtggtcggc ggcgatgtga atacagcggc gaatgcggct
      361 aaagtcgcga ttgaaaataa cctattatct caagaagagt atgctcttag agaaaaattg
      421 atcaaaaaag ccaaagggaa aggcctatta tctttagatt ggggcagcct gaccgaacaa
      481 gaggcaaggc agtttatcta tttgattgag aaagatcgat attctaatca attgcttgac
      541 cgatatcaaa aaaatccaag tagtttaaat aatcaagaaa aaaatattct tgcatatttt
      601 attaaccaaa cctctggagg taacacagct tgggcagctt cgatactgaa aacgccccag
      661 tcaatgggta atctcactat tccttccaaa gatattaata acaccttatc gaaagcctat
      721 caaacattga gtcgttatga ttcttttgat tacaaatcag ctgttgccgc acaacctgca
      781 ctttacttat taaacggacc gcttggcttc agtgtcaaag cagctactgt ggcagcagga
      841 ggatataaca ttggacaggg agcgaaagca atctctaatg gagaatatct gcatggtaca
      901 gttcaggttg ttaatggcac attgatggtt gcaggatctg tatctgcaca ggctgcaata
      961 tcggccaagc ctgcacctgt tacccgttat ctgagcaatg acagtgctcc tgctttaaga
     1021 caagctttaa ctgctgaaag ccagagaatc cgcatgaaac tgccggaaga gtatcgacaa
     1081 atagggaatc ttgcgatagc aaaaattaat gttaaaggat taccgcaaag gatggaagca
     1141 tttagttctt tccaaaaagg ggaacatgga tttatttcgt tacctgaaac aaaaattttt
     1201 aaacctatat ctgttgataa atatcataat attgcctctc ctcctagagg aacattaaga
     1261 aatatagatg gagaatataa attacttgaa actatagcac agcaactcgg aaataatcgt
     1321 aatgtatcag gtagaattga tctatttaca gaattaaagg cctgtcaatc ttgcagcaat
     1381 gttattttag agtttagaaa tcgctatcca aatattcaat taaatatttt tacaggaaaa
     1441 tagaatcatg gaaaaaataa aaaattatat agaattcaag aaattattta aaaatataac
     1501 ggaaaaattt gtcagagatt tagatataac attaattgaa gcagctgaat attctataat
     1561 tgaattcgaa atttatttat ctgaatatcc ggaagatagt acatttattt ttacagcatg
     1621 ggcaatctat ctgttagaaa ataactgtct caatgaaaga ggaaaagata attttttcat
     1681 cgcggaaatg aaaaaatgtt atcaacaaac taatttggat agaataaaat ctatctattc
     1741 ttcgagtgac tatcatattt ttgctaagga tgtaaacaag attcaacaaa catttaaatt
     1801 aaatcttgcc aatatcatgc agcaagagta gcaactgtat ttttaacccc gtcgggcaaa
     1861 attaccaaaa acggtaaatc actaaaaata cttttatacc gccgccgcag caagctgtag
     1921 cctgcatgaa acctaaaatc catgcgtaag gtgtgtgctc cagcacgcac gcgttccatg
     1981 atttacggct caatgccgtc tgaaaagctc acaatttttc agacggcatt tgttatgcaa
     2041 gtaaatattc agattcccta tatactgccc agatgcgtgc gtgctgaaga caccccctat
     2101 gcttgctgca gaactttcgg gtaaaaccgg tgtgagcatt agcgcaccgt atgccaatga
     2161 gaacagccgc atcattttga gtgccgccga tatagccgca gaaaacggca aaatcaaact
     2221 gcaatcctac ggcgaccaat cctattacgc cggacagggc gaactctata ccttagaacg
     2281 ccgcagctac aaaaccggca aatggtacaa ccgcaaacac attaccgaag tcaaagaaca
     2341 caaaaacgcc aagcccgacg cagtaaacct cagcgcatcc caaggtatcg acatcaaatc
     2401 tggtggcagc atcgacgcct acgccaccgt attcgatgcc cccaaaggca gcattaacat
     2461 cgaagccggg cggaaattga cactctatgc cgtagaagag ctcaactacg acaaactaga
     2521 cagtcataag aaacgcaaat ttctcggcat tacttatgat aaggtacatg atacctctac
     2581 ccaacagatg caaactgcct taccaagccg tgtagtggct gaatcagcta atctgcaatc
     2641 aggttgggat accaagttac aaggtacgca gtttgaaacc acgctgggcg gcgcggtcat
     2701 ccgtgcaggc gtaggcgacc aggcacgggc cgatgccaag attatcctcg aagggatcaa
     2761 aagcagcatc cacacagaaa ccgtaagcag cagcaaatcc gccctctggc aaaaacaggc
     2821 aggacgcggc agcaacatcg aaaccctgca actgccaagt ttcaccggcc ccgttgcgcc
     2881 cgtactctct gctcccggcg gcta
//
LOCUS       DAWKQB010000187         2786 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.188, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000187 DAWKQB010000000
VERSION     DAWKQB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.188"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..437
                     /locus_tag="WJL14_001953"
     CDS             114..437
                     /locus_tag="WJL14_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ7656970.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            551..2728
                     /locus_tag="WJL14_001954"
     CDS             551..2728
                     /locus_tag="WJL14_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ7656971.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKALDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNAEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          675 a          989 c          702 g          420 t
ORIGIN      
        1 gtaagccgca ggcttgcatc aaagtcgata acgctttcgt tacaactccg cccactgaac
       61 gaagccaaaa cgacaaaccg catcgtaaac cacccaaccc ataggagaac cccatgcaaa
      121 acgaaaccat caacctgaaa cagcaccttg ccgccatcaa agaatactgg cagcccgaaa
      181 tcatcaaccg ccacgggttc caattccgtt tggtcaaact tttgggcgat tacggctggc
      241 atacgcacgg atacagcgac aaagtgctgt ttgccgtgga gggcgacatg gcggtggact
      301 tcgccgacgg cggcagcatg acgatacgcg agggcgagat ggcggtcgtg ccgaagtcgg
      361 tgtcgcaccg cccgcgttcg gaaaacggct gctcgttggt gctgattgag ttgtccgacc
      421 cgtccgaggc cgtctgaaaa cgaagtttcc gaaggaagct gagtttctgc gaagctaaaa
      481 gcagcctgca ccttcaatca atatgccgaa aatacaaccc caccgcacac caacacacaa
      541 aggaaatccc atgacacgct tcaaatattc cctgctgttt gccgccctgt tgcccgtgta
      601 cgcgcaggcc gatgtttctg tttcagacga ccccaaaccg caggaaagca ctgaattgcc
      661 gaccatcacc gttaccgccg accgcaccgc gagttccaac gacggctaca ctgtttccgg
      721 cacgcacacc ccgctcgggc tgcccatgac cctgcgcgaa atcccgcaga gcgtcagcgt
      781 catcacatcg caacaaatgc gcgaccaaaa catcaaagcg ctcgaccgcg ccctgttgca
      841 ggcgaccggc accagccgcc agatttacgg ctccgaccgc gcgggctaca actacctgtt
      901 cgcgcgcggc agccgcatcg ccaactacca aatcaacggc atccccgttg ccgacgcgct
      961 ggccgatacg ggcaatgcca acaccgccgc ctatgagcgc gtagaagtcg tgcgcggcgt
     1021 ggcggggctg ctggacggca cgggcgagcc ttccgccacc gtcaatctgg tgcgcaaacg
     1081 cccgacccgc aagccattgt ttgaagtccg cgccgaagcg ggcaaccgca aacatttcgg
     1141 gctgggcgcg gacgtatcgg gcagcctgaa tgccgaaggc acgctgcgcg gccgcctggt
     1201 ttccaccttc ggacgcggcg actcgtggcg gcagcgcgaa cgcagccgcg atgccgaact
     1261 ctacggcatt ttggaatacg acatcgcacc gcaaacccgc gtccacgcag gcatggacta
     1321 ccagcaggcg aaagaaaccg ccgacgcgcc gctcagctac gccgtgtacg acagccaagg
     1381 ttatgccacc gccttcggcc cgaaagacaa ccccgccaca aattgggcga acagccgcca
     1441 ccgtgcgctc aacctgttcg ccggcatcga acaccgcttc aaccaagact ggaaactcaa
     1501 agccgaatac gactacaccc gcagccgctt ccgccagccc tacggcgtag caggcgtgct
     1561 ttccatcgac cacaacaccg ccgccaccga cctgattccc ggttattggc acgccgaccc
     1621 gcgcacccac agcgccagcg tgtcattgat cggcaaatac cgcctgttcg gccgcgaaca
     1681 cgatttaatc gcgggtatca acggttacaa atacgccagc aacaaatacg gcgaacgcag
     1741 catcatcccc aacgccattc ccaacgccta cgaattttcc cgcacgggtg cctacccgca
     1801 gcctgcatcg tttgcccaaa ccatcccgca atacggcacc aggcggcaaa tcggcggcta
     1861 tctcgccacc cgtttccgcg ccgccgacaa cctttcgctg atactcggcg gcagatacag
     1921 ccgttaccgc accggcagct acgacagccg cacacaaggc atgacctatg tgtccgccaa
     1981 ccgtttcacc ccctacacag gcatcgtgtt cgacctgacc ggcaacctgt cgctttacgg
     2041 ctcgtacagc agcctgttcg tcccgcaatc gcaaaaagac gaacacggca gctacctgaa
     2101 acccgtaacc ggcaacaatc tggaagccgg catcaaaggc gaatggcttg aaggccgtct
     2161 gaacgcatcc gccgccgtgt accgcgcccg taaaaacaac ctcgccaccg cagcaggacg
     2221 cgacccgagc ggcaacacct actaccgcgc cgccaaccaa gccaaaaccc acggctggga
     2281 aatcgaagtc ggcggccgca tcacgcccga atggcagata caggcaggtt acagccaaag
     2341 caaaacccgc gaccaagacg gcagccgcct gaaccccgac agcgtacccg aacgcagctt
     2401 caaactcttc actgcctacc actttgcccc cgaagccccc agcggctgga ccatcggcgc
     2461 aggcgtgcgc tggcagagcg aaacccacac cgaccctgcc acgctccgca tccccaaccc
     2521 cgccgccaaa gcccgcgccg ccgacaacag ccgccaaaaa gcctacgccg tcgccgacat
     2581 catggcgcgt taccgcttca atccgcgcgc cgaactgtcg ctgaacgtgg acaatctgtt
     2641 caacaaacac taccgcaccc agcccgaccg ccacagctac ggcgcactgc ggacagtgaa
     2701 cgcggcgttt acctatcggt ttaaataagg tcgtctgaaa acggagtttc tgcgaagcta
     2761 tagtggatta acaaaaacca gtacgg
//
LOCUS       DAWKQB010000188         2582 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.189, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000188 DAWKQB010000000
VERSION     DAWKQB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.189"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            31..510
                     /locus_tag="WJL14_001955"
     CDS             31..510
                     /locus_tag="WJL14_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ7656972.1"
                     /translation="MPSETAVADITRTYSAHYLNPNNRNMSLFPDALPCLDKLKAQGY
                     WLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGLDPKEA
                     LVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDDLSELPGFLAQHYA"
     gene            complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJL14_001956"
                     /gene_synonym="lytB"
     CDS             complement(586..1554)
                     /gene="ispH"
                     /locus_tag="WJL14_001956"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ7656973.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1583..2080)
                     /locus_tag="WJL14_001957"
     CDS             complement(1583..2080)
                     /locus_tag="WJL14_001957"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ7656974.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
     gene            complement(2286..2390)
                     /locus_tag="WJL14_001958"
                     /pseudo
     CDS             complement(2286..2390)
                     /locus_tag="WJL14_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          593 a          787 c          615 g          587 t
ORIGIN      
        1 ctgcccgaaa tcatccgcga cctgctcgaa atgccgtctg aaaccgccgt tgccgacatc
       61 acacgcactt attccgcaca ttacctcaat cccaacaacc gcaatatgtc cttatttccc
      121 gatgccctgc cctgtttgga caagctcaaa gcacaaggat actggcttgc cgtcgccacg
      181 ggcaaagggc gggcgggttt ggacaacgcc atcagccaaa ccgccaccgg cggctattgg
      241 ctcgccaccg cctgcgcggg ggaatatccc tccaaaccct cgcccgaaat ggtattcgga
      301 atctgcggcg aactgggact cgatccgaaa gaagcattgg tcgtcggcga tacggcgcac
      361 gacctgcata tggcggcaaa cgcaggcgcg gcggcagtcg gcgtggccac cggcgcacat
      421 tcgcgcgaac agctccttag cgcaccgcat ctcgccgtat tggacgactt gtccgaactg
      481 cccggttttc ttgcacaaca ttacgcctga ttggtttccg catcagacac tcgaaacaaa
      541 tgccgtctga agcctgttca gacggcattt gtgttgccca aacattcaac gtttgcacaa
      601 atcgggtttg gtttcgccct cgcggcgcag ctctttgggc aggacgaaca caatgctttc
      661 ttccgcaccc tcgccttcgc gtacggtttc gtgcccccat ccgcgtatgg ttgccagtac
      721 ttcccgcacc aacacttcgg gcgcggacgc gcctgccgtt acgccgactt tgtttttgcc
      781 ctcaaaccat gcgcgttgca ggtagcctgc attatccacc atatacgcat cgattccgcg
      841 cgatgccgcc acttcgcgca agcggttgct gttggacgaa ttgggcgaac cgaccacaat
      901 cacgatgtcg cactgttctg ccaactcttt gacggcggtt tgccggttgg tcgtcgcata
      961 gcagatatct tccttgtgcg gattgcggat attggggaaa cgcgcgttca gcgcggcgat
     1021 gatgtctttg gtttcatcga ccgagagcgt ggtttggctg acataggcga gtttgtcggg
     1081 gtttctgact tcgagttttg ccacatctcc gaccgtttcg accaaaagca ttttgcccgg
     1141 cgcaagctgc cccatcgtgc cttcgacctc gacgtgcccc ttatgcccga tcatgatgat
     1201 ttcacagtct tgggcatcca gtcgggcgac ttccttatgc actttcgtca ccagcgggca
     1261 agtcgcatca aacacgcgga aaccgcgctc cgccgcttct tgctgcaccg ccttcgatac
     1321 gccgtgtgcc gaataaacca gtgtcgcgcc cggcggcact tccgccaagt cttcaataaa
     1381 caccgcacct ttttcacgca ggttgtccac gacgaatttg ttgtgaacga cttcgtggcg
     1441 cacataaatc ggcgcgccga actcttccaa agcacgttcg acaatactga ttgcccgatc
     1501 cacaccagcg cagaagccgc gcggattggc aaggatgatg gttttctcgt tcataagccc
     1561 ggtatttcgt tttcagacgg catcaatatt tttcttcttg ggttttacgg tggacgatgt
     1621 tgtccaacac tgccaacacc gcgccgacgc agataaagct gtcggcaata ttaaaggcgg
     1681 gataaaacca attttgccaa taaaacaata agaaatcgac gacatgaccg tgtatcaggc
     1741 ggtcgatgac attgcctaac gcaccgccga taatcatcgc cgcacccgtt ttgccgaggg
     1801 ttgcaaactc atcgcgcaag atggcgcgta ccaaatacgc gctcaccgcc accgccagca
     1861 ccaaaaaaaa gtatttttgc cagccgccct gatcggcaag gaagctgaac gccgcacccg
     1921 ggttgtacac cagcgtcaga tcgaaaaagg aaggaatgac attgacgcgt tcccgatact
     1981 gaaacgacga cagcaccgcc cacttcgacc actggtccag cacgatggcg gcaagtgcca
     2041 atacccaata gcgcgtttta cttgaaacag atgaagacat atttttcaac agccggtaaa
     2101 agagtaccat tttacccgaa aacccccttt cctgtacccg aaacggcaaa tgccgtaatc
     2161 ttaaaacccg tcattcccga caacaccgta atctcgaaac ccgtcattcc cgcgtaggcg
     2221 ggaatccaga cctgtccgca cagaaactta tcggataaaa acagttgccc aaaccccgcg
     2281 ttctatagtg gattaaattc aaaccagtac ggcattgcct cgccttgccg tactatttgt
     2341 actgtctgcg gcttcgttgc cttgtcctga tttaaattta atccactata gattcccact
     2401 tccgtgggaa tgacggttca gttgcattcc gacaacaccg taatcttgaa atccgtcatt
     2461 cccgcgcagg cgggaatcta tcggaaatga ctgaaacctc gagattctag attcccactt
     2521 tcgtgggaat gacggttcag ttgcgttcca acaacaccgc aatctcgaaa tccgtcattc
     2581 cc
//
LOCUS       DAWKQB010000189         2561 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.190, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000189 DAWKQB010000000
VERSION     DAWKQB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.190"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            79..2292
                     /locus_tag="WJL14_001959"
     CDS             79..2292
                     /locus_tag="WJL14_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ7656975.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCTALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          691 a          806 c          601 g          463 t
ORIGIN      
        1 attttagtaa cctatgttat tgcaaaggtc tcaagctgtt acaataaact cgtttttatc
       61 ggaacggaag accccatcat gaccgccatc agcccgattc aagacacgca aagcgcgact
      121 ctgcaagaat tgcgcgaatg gttcgacagc tactgcaccg cgctgccgaa caacgataaa
      181 aaacttgtct tagccgcccg ttcgctggcg gaagcacatt accccgccga tgccgccacg
      241 ccgtatggcg aaccgctgcc cgaccacttc ctcggcgcgg cgcaaatggt tcatgaactc
      301 gacctgctcc ccgatgccgt cgccgccacc ctgcttgccg acatcggacg ctacgtcccc
      361 gactggaacc tattggtttc cgaacgctgc aacagtaccg tcgccgagct ggtcaaaggt
      421 gtggacgaag tgcagaaact cacccacttc gcccgggtgg acagcctcgc cacgccggaa
      481 gaacgcgccc agcaggcaga aactatgcgg aaaatgctgc tggcgatggt taccgacatc
      541 cgcgtcgtgt taatcaaact ggcgatgcgt acgcgcaccc tgcaattttt aagcaacgcc
      601 cccgacagcc ccgaaaaacg cgccgtcgcc aaagaaaccc tcgacatctt cgccccgctc
      661 gccaaccgtt tgggcgtgtg gcagctcaaa tggcagctcg aagatttggg cttccgccat
      721 caagaacccg aaaaataccg cgaaatcgcc ctgcttttgg acgaaaaacg caccgaacgc
      781 ctcgaataca tcgaaaactt ccttaatatc ctgcgtacgg aactcaaaaa atacaatatc
      841 cactttgaag tcgccggccg tccgaaacac atctactcca tttacaaaaa aatggtgaag
      901 aaaaaactca gcttcgacgg gttgttcgac atccgcgccg tgcggattct ggttgatacc
      961 gtccccgagt gttacaccac actgggcatt gtccacagcc tctggcagcc cattcccggc
     1021 gagttcgacg actacatcgc caacccgaaa ggcaacggct ataaaagttt gcacaccgtc
     1081 atcgtcggcc cggaagacaa aggcgtggaa gtgcaaatcc gcaccttcga tatgcaccaa
     1141 ttcaacgaat tcggtgtcgc cgcgcactgg cgttacaaag agggcggcaa aggcgattcc
     1201 gcctacgaac aaaaaatcgc ctggttacgc caacttttgg actggcgcga aaacatggcg
     1261 gaaagcggca aggaagacct cgccgccgcc ttcaaaaccg agcttttcaa cgacacgatt
     1321 tatgttttga ccccgcacgg caaagtcctc tccctgccca caggcgcgac ccccatcgac
     1381 ttcgcctacg ccctgcacag cagcatcggc gaccgttgcc gcggtgcgaa agtcgaaggg
     1441 cagattgtgc cgctgtccac cccgctcgaa aacggacagc gtgtcgaaat cattaccgcc
     1501 aaagaagggc atccttccgt caactggctt tacgaaggct gggtcaaatc caacaaggca
     1561 atcggcaaaa tccgcgccta catccgccag caaaacgccg acaccgtgcg cgaagaaggc
     1621 cgcgtccaac tcgacaaaca gcttgccaaa ctcacgccca aacccaacct gcaagagctt
     1681 gccgaaaatc tcggctacaa aaagccagaa gacctctaca ccgccgtcgg acaaggcgaa
     1741 atttccaacc gcgccatcca aaaagcctgc ggcacgctga acgaaccgcc gcccgtaccc
     1801 gtcagcgaaa ccaccatcgt caaacagtcc aaaatcaaaa aaggcggcaa aaacggcgtg
     1861 ctcatcgacg gcgaagacgg tctgatgacc acgcttgcca aatgctgcaa acccgcgccg
     1921 cccgacgaca ttgtcggctt cgttacccgc gatcgcggca tttcggtaca ccgcaaaacc
     1981 tgcccctctt tccgacacct cgccgaacac gcgcccgaaa aagtactgga cgcaagttgg
     2041 gcggcgttgc aggaaggaca agtgttcgcc gtcgatatcg aaatccgcgc ccaagaccgc
     2101 tccgggcttt tgcgcgacgt atccgacgcg ctcgcccgcc acaaactcaa cgttaccgcc
     2161 gtgcaaaccc agtcccgcga cttggaagcc agcatgaggt tcacgctcga agtcaaacaa
     2221 gttaccgacc tcccacgcgt cctcgccagc ctcggcgacg tcaaaggcgt attgagcgtt
     2281 acccggcttt aaatacaaaa atgccgtctg aaagccgtat aacgcttcag acggcatttt
     2341 gattgccggg gtttgctatt ttttgttgca taatcaatta aaaacaaaat agtacaatat
     2401 tcaactttga aggtctaacc atggcatact ctgcggactt aagaaacaaa gctttaaact
     2461 atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
     2521 gcaaggcaag gcaacaccgt actggtttaa atttaatcca c
//
LOCUS       DAWKQB010000190         2519 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.191, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000190 DAWKQB010000000
VERSION     DAWKQB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.191"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            358..792
                     /locus_tag="WJL14_001960"
     CDS             358..792
                     /locus_tag="WJL14_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656976.1"
                     /translation="MDNKKEFINNLTNRYMWIYSLVLNILFLPFYQSYQSFFIALGCL
                     FALVRKMQSLDFKLQNHIVLLNIKSAWADKKVFLIRIVVSWLAVMEIWMCFISESSTW
                     VCGAFCLNSEILEKIFRGFGYSGSLYFLFILMIDLNKLRESI"
     gene            1068..1787
                     /locus_tag="WJL14_001961"
     CDS             1068..1787
                     /locus_tag="WJL14_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656977.1"
                     /translation="MKNKNYLLVFIVLHIALIVINIVFGYFVFLFDFFAFLFFANVFL
                     AVNLLFLEKNIKNKLLFLLPISIIIWMVIHISMINIKFYKFEHQIKEQNISSITGVIK
                     PHDSYNYVYDSNGYAKLKDNHRYGRVIRETPYIDVVASDVKNKSIRLSLVCGIHSYAP
                     CANFIKFAKKPVKIYFYNQPQGDFIDNVIFEINDGNKSLYLLDKYKTFFLIENSVCIV
                     LIILYLKFNLLLYRTYFNELE"
     gene            1774..1947
                     /locus_tag="WJL14_001962"
                     /pseudo
     CDS             1774..1947
                     /locus_tag="WJL14_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
     gene            2005..2367
                     /locus_tag="WJL14_001963"
                     /pseudo
     CDS             2005..2367
                     /locus_tag="WJL14_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581420.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT          848 a          316 c          469 g          886 t
ORIGIN      
        1 tttgcagtgc gttatcttca tatttcgggg gtaacatatc tgctaatcta gtacagcctc
       61 aaacaaaaaa gagaaatttt aatttcgcta aatcgcataa agattaatca agagtatcat
      121 taaatgatat gagtgagcat ctataatgcc aagaagagtt gttaagacat aacgattatt
      181 gaaatagatt gtaaaataga tacttagata gtctgaaaaa cggatttgtg aaacttttta
      241 ttacgcgcca tcatttgaaa atgaaactta aaaaacactt atcataataa atattttctt
      301 tacgttgttt gctaataaac tcagtgcaat atcagcgcaa tattttatgg aaattttatg
      361 gataacaaaa aagaatttat taataattta acaaataggt atatgtggat ctattcattg
      421 gtcttaaata ttctatttct acctttttac cagtcctacc aatctttttt tattgcgctt
      481 ggttgtttgt ttgcactggt tagaaaaatg caaagcttag attttaaatt acaaaatcat
      541 attgtattgt taaatataaa aagtgcttgg gcagataaaa aagtattttt gattaggata
      601 gtagtgtcat ggttggcagt aatggaaata tggatgtgtt ttatttcgga atcatcaacg
      661 tgggtatgcg gtgctttttg tttaaatagt gaaatattgg aaaaaatttt tcgtggcttt
      721 ggttattctg gtagtttata ttttttattt atattgatga ttgatctcaa taagttaaga
      781 gagagtattt gaatttcatt ttttgtttga cttaaactca aggagagtaa caatgattgg
      841 tagtggtgat actaaacaat gcaaaaaatt ttctgcgtgt gatggaaaat accacgtcta
      901 cgatcccctc gccctagact tggacggcga cggcatagaa acagtcaccg ccaaaggctt
      961 ttcaggcagc ctgaagactg agagagtgaa tacgatgagt atacactcta tgccactaaa
     1021 ttgatattca ctaaatcata ccagctatat tttatttaat gagacatatg aaaaataaaa
     1081 attatttact agtatttata gttttacata tagccttgat agtaattaat atagtgtttg
     1141 gttattttgt ttttctattt gatttttttg cgtttttgtt ttttgcaaac gtctttcttg
     1201 ctgtaaattt attattttta gaaaaaaaca taaaaaacaa attattgttt ttattgccga
     1261 tttctattat tatatggatg gtaattcata ttagtatgat aaatataaaa ttttataaat
     1321 ttgagcatca aataaaggaa caaaatatat cctcgattac tggggtgata aaaccacatg
     1381 atagttataa ttatgtttat gactcaaatg gatatgctaa attaaaagat aatcatagat
     1441 atggtagggt aattagagaa acaccttata ttgatgtagt tgcatctgat gttaaaaata
     1501 aatccataag attaagcttg gtttgtggta ttcattcata tgctccatgt gccaatttta
     1561 taaaatttgc aaaaaaacct gttaaaattt atttttataa tcaacctcaa ggagatttta
     1621 tagataatgt aatatttgaa attaatgatg gaaacaaaag tttgtacttg ttagataagt
     1681 ataaaacatt ttttcttatt gaaaacagtg tttgtatcgt attaattatt ttatatttaa
     1741 aatttaattt gcttttatat aggacttact tcaatgagtt ggaatagttt tggtaatttt
     1801 atgagcgcac gctcatccgc gttagcagaa tttggaaata tggttgctaa tttagtttct
     1861 gcaaaaaatg agaaagatat ctcgaaacgt aatgaatatt acaaacaagc tggttatagt
     1921 gcattattag catttggtaa tttggctagt aatattgcac caggtagtac gtcatcgcat
     1981 attgtaaacg gaacaaatgc ctctgtgatt gcaagccgtc tctctggaaa tatatcttca
     2041 gctattcagg agcataaaga tggtaaagtt aatatcaacc gttttcaaaa tattttagcg
     2101 gatttatatt cattgggagg gttaggaagt acattaatag agaagaatgg aaatatgcag
     2161 agttggggga ttccattagc aattgctgga gatataattg cagcaacggc tattgccaca
     2221 ggagatactg gtacgatatc tacagaggaa ttttataatt ttgacaactg gaaaggtttt
     2281 gggtatgagc tatttgaaga ctggtctcgt tgggtatacg actggctgcc cgacggctgg
     2341 aatctgtgga aagaattgga cagaaaccgt tgttggattc ttaccgaaag agatggaagg
     2401 cgacaattta aatggttagg taagccagat cctacgcgcg gctggtttga taacggacac
     2461 gaatgcttga aaccttgggg taacatattc gattcttatc gccgtggcaa atatcatgt
//
LOCUS       DAWKQB010000191         2498 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.192, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000191 DAWKQB010000000
VERSION     DAWKQB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.192"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            169..792
                     /gene="lolA"
                     /locus_tag="WJL14_001964"
     CDS             169..792
                     /gene="lolA"
                     /locus_tag="WJL14_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ7656978.1"
                     /translation="MMKPHNLFQFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1065..2204
                     /locus_tag="WJL14_001965"
     CDS             1065..2204
                     /locus_tag="WJL14_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ7656979.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAHPPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2337..>2498
                     /locus_tag="WJL14_001966"
     CDS             2337..>2498
                     /locus_tag="WJL14_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7656980.1"
                     /translation="MDLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYID"
BASE COUNT          675 a          727 c          617 g          479 t
ORIGIN      
        1 cggcacacat taaagggcag cgtggcgcgc ctgccttttc cgagcggtca aaaaaatcag
       61 ccctcggaaa acgcggtttg caaaatacaa actgcccgta acgccgcccg tatgattgtt
      121 ttgccgcgcc gatactttac gccacactca ttccaacaag gaaaaataat gatgaaaccg
      181 cacaacctgt tccaattcct cgccgtttgc tccctgaccg tctccgtcgc ttccgcacag
      241 gcgggcgcgg tggacgcgct caagcaattc aacaacgatg ccgacggtat cagcggcagc
      301 ttcacccaaa ccgtccaaag caaaaagaaa acccaaaccg cgcacggcac gttcaaaatc
      361 ctgcgcccgg gcctctttaa atgggaatac acttcgcctt acaaacagac tattgtcggc
      421 gacggtcaaa ccgtttggct ctacgatgtc gatttggcac aagtgaccaa gtcgtcccaa
      481 gaccaggcca taggcggcag ccccgccgcc atcctgtcga acaaaaccgc cctcgaaagc
      541 agctacacgc tgaaagagga cggttcgtcc aacggcatcg attatgtgct ggcaacgccc
      601 aaacgcaaca acgccggcta ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc
      661 gccatgcagc ttaaagacag cttcggcaat caaacctcca tcagtttcgg cggtttgaat
      721 accaatcccc aactctcgcg cggcgcgttc aagtttaccc cgcccaaagg cgtggacgtg
      781 ttgagcaact gatgccgtcc gccccgatgc cgtctgaaag ccgccgaggc ttcagacggc
      841 attttcacgc aggcggaaca atgtcccgca ttaccgcccg atcgggcatc ggaacggcaa
      901 acccgtgaaa attaacggtt gcgccccggt tgtttttgcc gtttaatgca aaccttgctg
      961 catcaagggc caagaaagcc gaccggccgc ccccacagct tccgatgcag gcggccaggc
     1021 cgtccctgca atgtttttta tttttgaacg aaaggtcgaa aaccatgaaa aaaacactgg
     1081 tggcggcggc aatcctgagc ctcgccttga ctgcgtgcgg cggcggaaac gataccgccg
     1141 cccacccccc ctccgccaag cccgaagccg aacaatcggg caaactcaat atctacaact
     1201 ggtcggatta tgtcgatccc gaaaccgtcg ccgcctttga aaaagaaacc ggcatcaaga
     1261 cgcgttccga ttattacgac agcaacgaaa cactggaggc aaaagtcctg accggcaaat
     1321 ccggctacga cctgaccgcg ccgtccatcg ccaacgtcgg ccggcaaatc aaagcgggcg
     1381 cgtatcagaa aatcgacaag gcgcaaatcc cccattacgg caacatcgat aaagatttgc
     1441 tgaaaatgat ggaagccgtc gatccgggca acgaatacgc cgtcccctat ttctggggca
     1501 tcaacacctt ggcaatcaac acgcggcagg tgcaaaaggc attgggtacg gacaagctgc
     1561 ccgaaaacga atgggatttg gtgttcaacc ctgaatacac cgccaaactc aaatcctgcg
     1621 gcatcagcta tttcgacagc gcaatcgaac agattccttt ggcgttgcac tatttgggca
     1681 aagaccccaa cagtgaaaat cccgaagaca tcaaagccgc cgtcgatatg atgaaagccg
     1741 tccggggcga cgtgaaacgc ttcagctctt ccggctatat cgacgatatg gcggcgggca
     1801 acctgtgtgc cgccatcggt tacggcggcg atttgaacat tgccaaaacc cgtgccgaag
     1861 aagccgcaaa cggcgtggaa atcaaagtat tgaccccgaa aaccggcgtg ggcgtgtggg
     1921 tggattcctt tatgattccg cgcgacgcgc aaaacgtcgc caacgcccac cgctatatcg
     1981 actacacgct ccggcccgag gtggcggcga aaaacggcag cttcgttacc tacgcgcccg
     2041 ccagccgtcc cgcgcgcgag ctgatggatg aaaaatacac ctccgacgca tcgattttcc
     2101 cgaacaaaga actgatggaa aaaagtttca tcgtatcgcc caaatccgca gaatccgcca
     2161 aattgggcgt gaagctgtgg caagggctca aagcgggcaa ataaccggaa tccctgccgt
     2221 ctgaaacctt tcgggcggca ggaaacggcg cgtccgttat caaacagggg ggcgtttccc
     2281 ctcctgccgg ttatgattgg gttaagatta aaatgattta gtaaaatgag aaagatatgg
     2341 atttaagtat cgtagttccc atttataatg tcgaaagtta tttggaagcg tgtttaagtt
     2401 ccatagaacc tatattaagt aatgaaaatg tcgaacttat ccttgtgaat gacgggtcaa
     2461 aagacggaag tgaagatata tgttataaat atatagat
//
LOCUS       DAWKQB010000192         2482 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.193, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000192 DAWKQB010000000
VERSION     DAWKQB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.193"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(26..832)
                     /locus_tag="WJL14_001967"
     CDS             complement(26..832)
                     /locus_tag="WJL14_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ7656981.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILNR"
     gene            1053..2423
                     /gene="ffh"
                     /locus_tag="WJL14_001968"
     CDS             1053..2423
                     /gene="ffh"
                     /locus_tag="WJL14_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ7656982.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFINNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTVGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEAAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRLAKGMKGMK
                     GMFPGL"
BASE COUNT          683 a          625 c          636 g          538 t
ORIGIN      
        1 ttcggacggc atctgttggc tcttcttatc tgttcagaat gatttccaat acgaacttgc
       61 tgcccatata ggcaatcatc aggctgacaa atccgatgat ggtccacacg gcggcttttt
      121 tgccgcgcca tgcggtcatg ctgtgcttga gcagcagtcc gccgtaaatc agccatgaca
      181 atatgccgaa tacggtttta tgggtaaagg tcatgggttt gccgaatacg gcttcggcaa
      241 aaaatgttcc gctgacgacg gaataggtca gcaggatgaa acctgcccac atagcctgga
      301 acatgagttt ttccaaactg agcagcgacg gcaggaatcc tgcgagcttg gagaagttcc
      361 tgcggtgcag gctccgattc agcagcaggg tcaaaacgga caataatgtt gcgatgccga
      421 acagcccgta tgcaagcagc gaagttccga tatgcagcat aaagggaagg tcggtaattt
      481 catatcccga gaattttcca ggaaaaacca aacctgacag cagcatcagt gcggcgcaag
      541 gatacagcag caactgcact ccgcgcagcg aataaaagaa gctgccggca aaataaataa
      601 acagcatcat ccaaacaatc aggctgccgg aatacccaaa gcccataatg atgattttgt
      661 cttgaatgac cggcataagc agtgccgcgc catggacggt caatgccgcg cccaaaaccg
      721 gcaattccgt cttccacggg taatcccggc cgagcccctg ctgttggcag tgccatgcaa
      781 atgcacccaa tcctgcgtaa accgccgtca aaaagatgaa aactgtcggc atggtggact
      841 ttctctaatc tatactgttg cgctgtatgc ggccgcttat gaaatattgg aacttttaac
      901 gttggaattg taaaatcccc gtttcgggca agccttgacg gatttgccga tatgctgtcc
      961 ggcacacaag ccgcatcaaa ttattttgat tttattttaa caaagaatgc ccctgatggg
     1021 gcaagctatt cttattcaga ccaaggacca gtatgttaga caatttaacc ggccgcttca
     1081 gcaatgttct caaaaacatc cgcgggcagg ccaaactgac cgaagacaat attaaagaag
     1141 ccttgcgcga agtccgcctc gccctgcttg aggcggatgt cgccctgcct gtcgtcaaag
     1201 agttcatcaa caacgtcaaa gaaaaggccc tcggtcagga agtagcgggc agcctgacgc
     1261 cggatcaggc atttatcggc gtggtcaaca aagccctgac cgaactgatg ggcagggaaa
     1321 acaaaacgct ggatttgtcg gttgcgccac ccgccgtcgt gttgatggcg ggtttgcagg
     1381 gtgccggtaa gacaacaacc gtcggcaaac tcgcccgcct gctgaaaaac gatcagaaga
     1441 aaaaagtttt ggtggtatct gccgacgttt accgccctgc cgcgattgaa cagctgcgcc
     1501 tgttggctga acaggtcggc gtggactttt tcccatccga taccaaccaa aaaccggttg
     1561 aaattgccac tgccgccgtc gattacgcca aaaaacattt ttacgatgtc ttaatggtcg
     1621 ataccgccgg ccgtttggca atcgatgaag agatgatgaa tgaaatcaaa gcccttcatg
     1681 ctgcggtcaa cccggttgaa accttgtttg ttatcgatgc catgttgggt caggatgcgg
     1741 tgaacactgc tcaggcattt aatgaagccc tgccgctgac cggtgtcgta ttgaccaaga
     1801 tggacggcga ctcgcgcggc ggtgcggcat tgtccgtacg ccatgtaacc ggcaaaccga
     1861 ttaaatttat cggtgtcggc gagaaaatca acggcctcga acctttccac cccgaccgtc
     1921 ttgccagccg tatcctcggc atgggcgacg tattgaccct gattgaagac gttcaaaaag
     1981 gtattgatga agccgccgcc gccaaaatgg cgaaaaagct gcaaaaaggc aaaggcttcg
     2041 atctcaatga ctttaaagaa caaatccagc agatgcgcaa tatgggcggc ttggaaaacc
     2101 tgatgtcaaa aatgccgggc gaactgggtc aaatatcgaa acaaatcccc gaaggtacgg
     2161 ctgaaaaagc gatgggcaaa gtggaagcca tcatcaactc gatgacccct aaagaacgcg
     2221 ccaaccctgc cctgctcaaa gctagccgca aacgccgtat tgcgatgggt gcgggtacga
     2281 ccgtgcagga agtgaataaa ttgctcaaac agtttgaaca aatgcaacaa atgatgaaga
     2341 tgttcagcgg caacggcttg ggcaaactga tgcgcttggc caaaggcatg aagggaatga
     2401 aaggcatgtt cccaggtttg taagccgatt tgacagaaaa cgccgtctga aatttcagac
     2461 ggcgtttttg ttttatattc tg
//
LOCUS       DAWKQB010000193         2475 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.194, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000193 DAWKQB010000000
VERSION     DAWKQB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.194"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            35..1390
                     /locus_tag="WJL14_001969"
     CDS             35..1390
                     /locus_tag="WJL14_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ7656983.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGYYSDRTWMPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WJL14_001970"
     CDS             1394..2404
                     /locus_tag="WJL14_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ7656984.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     SYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          459 a          669 c          734 g          613 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggtt attactccga ccgcacttgg atgccgcgat tgggcggtcg
      301 ccgcctgccg tatctgtttt acggcacgct gattgcggtc atcgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctacgc ttctttggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgctggacg tgtcttccaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaga gctttttggc
      541 gaataccggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccaca aaccgtggtc gtagcattct atgtgggcgc
      661 ggcattactg attattacca gtgcgttcac aatcttcaaa gtcaaagaat acgacccgga
      721 aacctatgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaacttttg aaaaccgcgc ccaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc tttcaatata tgtggactta ttccgcaggc gcgattgcgg aaaacgtttg
      901 gcacaccacc gatgcgtctt ccgtaggcta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtgacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgcctcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tccctatgct gggcggcttg caggccacta tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttctgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat tttggcctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgac gatgcggctt cctatgcgga caatccgttt cagattaaca agcagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttagctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acgacctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgccgtcg tatcagcata tacagcgacc gcaatggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgaat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgtagacg gcctttttat agtggattaa caaaaactgg
     2461 tacggcgttg cctcg
//
LOCUS       DAWKQB010000194         2456 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.195, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000194 DAWKQB010000000
VERSION     DAWKQB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.195"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..380)
                     /locus_tag="WJL14_001971"
     CDS             complement(<1..380)
                     /locus_tag="WJL14_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656985.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WJL14_001972"
     CDS             complement(438..803)
                     /locus_tag="WJL14_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656986.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WJL14_001973"
     CDS             complement(785..1330)
                     /locus_tag="WJL14_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656987.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="WJL14_001974"
     CDS             complement(1493..1765)
                     /locus_tag="WJL14_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656988.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          774 a          552 c          366 g          764 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccccctctg tctccttcat ctccttttga gaaagctccg
     1201 ccaactgcat cggtgcatct gcccgcgtgt ggaacacttg gtcttcaaat gcctgattgt
     1261 ccaagccgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtgattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt gagcctagac ccaatctacg tttgctgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tatattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atgcaatata gtgaattaac
     2401 aaaaaccagt acggcgttgc ttcgccttag ctcaaagaga acgattctct aaggtg
//
LOCUS       DAWKQB010000195         2361 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.196, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000195 DAWKQB010000000
VERSION     DAWKQB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.196"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(318..1397)
                     /gene="aroB"
                     /locus_tag="WJL14_001975"
     CDS             complement(318..1397)
                     /gene="aroB"
                     /locus_tag="WJL14_001975"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ7656989.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIITNET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMTGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGTLGDISFFEWLEQHMPELMALERAPLIQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAVAAGCVLAA
                     RLSEQLGKISAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            complement(1477..1989)
                     /locus_tag="WJL14_001976"
     CDS             complement(1477..1989)
                     /locus_tag="WJL14_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ7656990.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
BASE COUNT          496 a          564 c          727 g          574 t
ORIGIN      
        1 ggaacttatc ggaaacgacc gaaaccgaac ggactggatt cccgcttttg cgggaatgac
       61 gaggttttaa gttgctgttt tgggtttctg ttttttgtgg aaataacgag attctagggt
      121 tgcagaaact tctccgaaac aacaaaaacc tttccaccgt cattcctgcg aaagtgggaa
      181 tctagaaatg aaaagcaacg ggaacttatc ggaaacgacc gaaaccgaac ggaccggatt
      241 cccgcttttg cggagaaaga tgatggaaaa tcatcatcgt gcgtcaaacc cgcgcggcag
      301 cacatcggca gaggaaatca gagatacggt tgcagggtgc ggcggaggat gtccgtgtcg
      361 gtaatttcgg tgatgttggc ttcgcccagc cggttcagac cgataaagcg catgatgccg
      421 ccgctgactt ttttatcgtg gctcatgtgt tccagccatt tttcaaaggc aaacacgggt
      481 ggcgcggacg gcagtccggc ggcttcgagc agggcggcga gccgcgcggt atctgcggcg
      541 gagattttgc ccagttgttc ggacaaacgc gccgccaaca cgcagccggc ggcgacggcc
      601 tcgccgtgca gccaagtacc gtaacccatc tcggtttcaa tggcgtgtcc gaaggtgtgt
      661 ccgaggttga gccatgcgcg tatgccctgt tcggtttcgt cttgggcgac gatgtctgcc
      721 ttcatttggc aacagcggta cacggcttgg atgaggggcg cgcgttcgag tgccatcagt
      781 tcgggcatat gctgttccag ccattcaaaa aaactaatgt cgccgagcgt gccgtatttg
      841 atgacttccg ccataccggc ggacagttcg cgggcgggca gggtgtgcag cgtgtccaag
      901 tcggcaagca ccgcctgcgg ctggtagaac gcgccgatca tatttttgcc gagcgggtgg
      961 ttgatggcgg ttttgccgcc gacggaggaa tcgacctgac ttaataaagt agtgggaatt
     1021 tgaacaaatg gcgcgccgcg ttgataagta gccgccgcaa aacccgtcat atcgccgatc
     1081 acgccgccgc ccagtgcgat gagcgtggtt ttgcgttcgg cgcggttttg catcagcccg
     1141 tcaaagatga ggttgagcgt ctgccagttt ttgtgcgcct cgccgtcggg caggatgatg
     1201 ctgaaatggg atacgcctgc cgcatccaat gccgtctgaa gcgtgccgag gtagagcggg
     1261 gcgacggttt cgttggtgat gatggcggcg cgtttgccca aatgcggttt gagcaggctt
     1321 cctgcctgcg gcagcagtcc gttgccgata aagatggggt agctgtggga cggggtgtgt
     1381 acggtcagtg ttttcattgt tgttccttaa agtttgaacc gccggcccgc cgggcggggc
     1441 gcggtttggt tttctgggcg cggcgcatat gccggtttat cgggataagc gtttgagcag
     1501 ggtttgcacg gtttcccggc agtttgccga ttctacggta aagtcggcgg tttggcggta
     1561 aacggggtcg cgtgcggcgt agagttcacg taatttcgcc aaaggatcgg caacttgcag
     1621 caaaggacgg ctgttgtcgc agcgcgtgcg ttcgagcagg gtttcgggcg gggcgtgcag
     1681 atagacgacc gtgccgcttt tgcggataag ggcgcggttt tcttctttta acaccgcgcc
     1741 gccgccggtg gacaggacga tatggggcag gataaccagc tttttgagta tggcggtttc
     1801 gcgcgaacgg aatccctgtt cgccttccat ttcaaatatg gtggggatgg gaacgccggc
     1861 ggctgcggcg atttcgtgat cgctgtcgta aaaacggtaa tccagccgct gcgccatttg
     1921 ccggcccagc gtggttttgc ccgcgcccat cagtccgatg aggatgagtt tgccgttaaa
     1981 gtttttcatc acaattcctt aatgtttgac accccgcctt tcggggcgac agggttcggg
     2041 cttgtcgggt tacggcggaa ttttatacga aatcggcagg gcggcggtac gtttggaaaa
     2101 ataacccgac catcccgaac ctttctgatt ttaaggacaa ataaaagaaa tcagggaggt
     2161 tttttatttc aggctgtgtt ttgacaatcc gttgatttca cttatttgtc aggaaaaggc
     2221 aattatcttt gcttaggtaa acaattatcc aattgaatat attgaagata atatgtttat
     2281 caatactata gtggattaac aaaaaccagt acagcgttgc ctcgccttgg ctcaaagaga
     2341 acgattctct aaggtgctga a
//
LOCUS       DAWKQB010000196         2331 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.197, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000196 DAWKQB010000000
VERSION     DAWKQB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.197"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..1247
                     /locus_tag="WJL14_001977"
     CDS             30..1247
                     /locus_tag="WJL14_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7656991.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGVSQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLTLPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1450..1590)
                     /locus_tag="WJL14_001978"
     CDS             complement(1450..1590)
                     /locus_tag="WJL14_001978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656992.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1603..>2331
                     /locus_tag="WJL14_001979"
     CDS             1603..>2331
                     /locus_tag="WJL14_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ7656993.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILITLYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDK"
BASE COUNT          544 a          727 c          537 g          523 t
ORIGIN      
        1 gcaaacttaa ccgactcaaa atacacatta tgacgcacac cgcatcgaaa acacccaaat
       61 tttggctgac cattaccgcc gccgcattca tcctgctgat taccatcggc atgaggatga
      121 cgctcggact gttcgtccaa cccgtcgtca acaccaccga attgagcatt gcccaattca
      181 gcctcatcat cgccgttttc caactgatgt ggggtgtatc gcaaccattg tccggcgcgc
      241 ttgccgaccg tttcggcgcg ttcagggtat taagcggcgg cgccgtcctg ctcgtctgcg
      301 cctgcctgat tgcccccaac atcccgactt attgggggct gatgattgcc gtcgggctgc
      361 tgctcgcatt cggcaccggt tccggcggtt tctccatcat tatggggcag gtcgccgccc
      421 aagtccccgc acacaaacgc ggcttggcat ccggactggt caacgccggc ggttcggcag
      481 gacaattcct gttcgcaccg ctggttcaag gactcgtcgt cctgcccgaa gtcggctgga
      541 cgggcacatt ttacgtttgg ggcgcaatcg ccctgctgac tctgcccgtc tcatggtggc
      601 ttgccggcgg caacaacgcc gcccacaccc aacacgccca agccacacac ggacaaagcc
      661 tcggagaagc agtcaaaacc gccttcaaaa ccccaagcta catcctgctg cacctaagct
      721 tttttgcctg cggcttccac atcgcctttc tcgtaaccca cctacccacg gaagtcgccc
      781 tgtgcggact gcccgccacc gtcgcctcga catccatcgc catcatcgga ctggcaaaca
      841 tcgccggctg cattttttcc ggctggtgca caagccgcgt tcggggcaaa tacgtcctgt
      901 tcggactgta cgcctcgcgc gccgcgatga tactcatcta cattttttcg cccaagaccg
      961 acctcaactt ctacattttc gccgccgcac tcggatttac ctggctcgcc accgtcacgc
     1021 cgaccgcctc cattaccggc aaactcttcg gcacacgcta cctcgccacc ctgttcggac
     1081 tgacgatgct cagccaccaa atcggcggat tcctcggctc gtacatcggc ggcatcgtga
     1141 ttacacaatt cggcgactac ggctggatgt ggtacgccga cgcactgctg gcgggaaccg
     1201 ccgccctcct caacctcccc atccgcgaac cccgaaccgc cgcatgaaag aatgccgtct
     1261 gaaaaccctc agacggcatt ttccaaacac cccgcccgtt tccggactgc acgacacgca
     1321 aatcagcctt atcaaaccgc aaacaaccgc cgcgattaaa ccgccattca aataagtccg
     1381 ttttcccttt gggtgtaaaa tcatcacaaa acgcgatact gtatatcgcc gggcgttatg
     1441 cagcggattt caggataaaa tgccgtctga agccgcccct gcccgggcgg gcgaagcgtc
     1501 ggatgcggtg cgcttggcaa aggtttgccc gccgcgcccc gtatccggca tttcatcaat
     1561 gcagaccgca tttccaatcc tataattcaa ggtgtccgcc ttatgaaaat cgtttttatc
     1621 acaacagtcg catccagcat ttacggtttc cgcgcccccg tcattaaaaa attaatcggc
     1681 aaaaaccatc aggtgtatgc ctttgtatcg gagttttccg acaacgaatt ggacattatc
     1741 agggaaatgg gggtaacacc cgttacctac cgttcaaacc gcagcgggct gaacccgttt
     1801 tccgatataa aatccacctt cctcatcttt aaagaactca aaaaaatatc gccggatttg
     1861 gttttccctt atttcgcaaa acccgtgatt ttcggcactt ttgctgcaaa attggcaggc
     1921 gtgcccagaa tcgtcgggat gctggaaggt ttgggattcg catttacccc gcagccggaa
     1981 ggcataccgt taaaaacaaa aatcataaag gggattttaa tcaccttgta ccgcattgcc
     2041 ctgccgatgt tggaaagcct gattgtatta aaccccgacg acaaagacga actgacggac
     2101 aaatacggca tcaaaataaa aaacatccat attttgggcg gaatcggtct ggatttgcgg
     2161 caatatcctt attccgaggc ggatattccc gatgaaaaag aacccgtaaa attcctcttt
     2221 atcggcagat ttctgaaaga aaaggggatt gatgatttta ttcgggcggc ggaacaggtt
     2281 aaggacaaat accccgatac ggtttttacc gctttgggcg caatcgacaa a
//
LOCUS       DAWKQB010000197         2313 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.198, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000197 DAWKQB010000000
VERSION     DAWKQB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.198"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WJL14_001980"
     CDS             complement(59..1468)
                     /gene="dacB"
                     /locus_tag="WJL14_001980"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ7656994.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1633..2199
                     /locus_tag="WJL14_001981"
     CDS             1633..2199
                     /locus_tag="WJL14_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ7656995.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          529 a          564 c          665 g          555 t
ORIGIN      
        1 agtttgtcct agttatctag gacagcccct taaatttaat ccactatatt ttcctgtttc
       61 aggctcggcg ttctttgcac atcagtttcg catccagcca gccatcgccg ccggagatga
      121 tgttgttggc aacgaagttg tccaagtcgg gcagcaggga aacggcgcgg ccgctgttga
      181 tgatgacgac caccgccatc ggtttgtcgc ccagccaata acctgcaagg gcgcggacat
      241 tgttgagcgt gccggttttt aagcgcaaca gcccgccgct ttgtttgaag cggttgcgta
      301 aagtcccgtc tgtgccggcg atgggcagcg tatcgatgaa atcttgtgca aacgggctga
      361 aataagccgt ttccaacatt tgcgccatca ttctcgccgt taccctttct tttctggaca
      421 gacctgaacc gttttccaaa accaaatccg caacatcgat gcccgacacg gcaagttcac
      481 gccggacggc agacgctgcc tgttcggaaa cggcgggcag tttgccgtcg ccgccgagtt
      541 tgaggaagac ggaacgcgca attagattgt ccgaacgctt gttcatgtcc gtcaaaattt
      601 ccttcatcgg ctttgagtgt gcaacggcaa gcgtctgcgc gccttccggc gtgtcggata
      661 tgccgatgcc gtctgaaatc cgtccgccgc cgagcagcca gtggttggta aaactttgcc
      721 ggatcagttc gtcaagcgcg aacatccgga caccgacagg cttgcccaaa cagctctcgg
      781 gaatattgcc gcgcaatttc agcgtattgt ccgaaaaaga tgcacgcatc agttttttga
      841 tcgaagggca ggcagcttgg gaggcggtaa ttttcaagtt gttttgggcg aaaatatgcg
      901 gcaaaggcgg atcggtgagg atgtcggtac tgtcggcggc attgcgttcg gcgcgcacca
      961 taaccatacc ggcagacagc atagttggat tggggggcgt cataaacggc gaaccgctgt
     1021 cggcttcgaa atcgtcgggg ctgccgactt cgccccacag gctgtggtcg agcatcaggt
     1081 gtcccgtgat attgcgtatg ccttgttcgc gcaactggcg ttggacggca agcaggtttt
     1141 cctgattgaa aacggggtcg ccgctgcccg cccaatacag gtttccgtca agcgtgccgt
     1201 cgtttaccgt accgttgctt ttaaactcgg tcgcccagcg gtaattgctg ccgaaggttt
     1261 tgaaggcggc aaacgcggta acgagtttca ttgtggaggc ggggttgacg gggacatccg
     1321 agcggtggtc aatgatgact tttccgctgt caagctcttg gacatatacg gcgatttcgt
     1381 tttgcggaat gcgacctgta tcgagcgcgt gtgcggcgag ggaggcgaga agcagcagca
     1441 gggaggccgc tgttttgggg aaattcatag gtaaatctgt tacataaaaa aagcaattat
     1501 aaggcaaagc cggataagtg ggaacgctgt ggcggcgggc cggcttgttt gcaggggaaa
     1561 tcacatatat aataatcgct atcattatga aatgattgag acgcacaaac ttaatattca
     1621 ggaggaatga ttatggctaa gaaaatcagt attttggtgg gcagcctgcg ccgtgcttct
     1681 tttgcgcgca aagtggcatt gaatgcggcg gagatgttcc ccgaaggctg gcaggcggaa
     1741 atcgtcgaaa tcggacatct gccgctttac aatttcgatt atgacgaccc tgcggtggaa
     1801 gatgtgccgc tgcccgaaag ctacacggtt ttccgcgaaa cgattaaggc ttcggacggc
     1861 attttgttcg ttacgtccga aaataaccgc accattcccg cctgtttgaa aaatgcggtg
     1921 gacatcggct cgaaaccgaa tgccgacgtg gcgtggaaaa acaaaccggc cggcatcatc
     1981 agccattccg tcggcaagat gggcggttac agttcgcaaa aaaacctgcg ccttgcgctg
     2041 tcttatttcg atatgcccgt aaccggacag ccggaagtgt ttttgggcaa ttcgccgaca
     2101 ctgtttgatg aaaacggcaa attgatcgac tcggcaaggg attttgttca gtcatatatt
     2161 aatcagtttg tcggtttgat tgaaagaaat gccaaataaa caaacaagtc gaaaggccgc
     2221 aaaaccggtt cgggttttgc ggctttttta tatgggctgg gcagcgtcaa ggtttgccgt
     2281 ttgccggata atgcgcggca aatgaccggc gtg
//
LOCUS       DAWKQB010000198         2289 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.199, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000198 DAWKQB010000000
VERSION     DAWKQB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.199"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..2129
                     /locus_tag="WJL14_001982"
     CDS             <1..2129
                     /locus_tag="WJL14_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7656996.1"
                     /translation="NANNPNKSTKAPSPALTAPALWFGPVQNGKVQMYSASVSTYPDS
                     SSSQIFLQNLSRKDDTSKPGRYSLKPLSTSEIKSKEPNFTGRQTIIRLDSGVREIKLD
                     RSNEATGLNGNDGKNETFGIVSEGSFMPDASEWKKVLLPWTVRGFADDSKFKAFNKEK
                     NNDNKPKYSQKYRSRDSSKHERDLGDIVNSPIVAVGGYLATSANDGMVHIFKKGNGGD
                     ERNYSLKLSYIPGTMPRKDIQSQESTLAKELRAFAEKGYVGDRYGVDGGFVLRRITDD
                     QDKQKHFFMFGAMGFGGRGAYALDLSKIDNSNPAGVSMFDVKNDNGVKLGYTVGTPQI
                     GKTHNGKYAAFLASGYATKDINNGENKTALYVYDLENNNGTPIAKIEVKDGKGGLSSP
                     TLVDKDLDGTIDIAYAGDRSGKMYRFDLSGNDPTKWSVRTIFEGDKPITSAPAISQLK
                     DKRVVIFGTGSDLSEEDVDKKDEQYIYGIFDDDTETTGNVKVDLKGLGGGLLEQHLTQ
                     EDKTLFLTDYKRSDGSGSKGWVVKLQPGQRVTVKPTVVLRTAFVTIRKYKDNGCGAET
                     AILGINTADGGKLTKKSARPIVPAANSKVAQYSGDKKTSSGKSIPIGCMEKDNGIVCP
                     NGYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGTFKEGKKPARNNRCFSGKGVRTLL
                     MNDLDSLDITGPTCGMKRISWREVFY"
BASE COUNT          654 a          574 c          600 g          461 t
ORIGIN      
        1 tcaacgcaaa taaccccaat aagagtacca aagccccttc ccccgccctg accgcccccg
       61 cgctgtggtt cggacctgtg caaaatggta aggtgcagat gtattccgct tcggtttcta
      121 cctaccccga cagttcgagc agccaaattt tcctgcaaaa cctttcccgc aaggatgaca
      181 caagcaaacc gggccgctat tccctcaaac ccttgagtac gtcggagatt aaaagtaaag
      241 agccgaattt cacggggcgg caaacaatca tccgattgga tagcggcgta cgtgagatca
      301 aactggatag aagcaatgag gccaccggtt taaatggaaa tgacggcaaa aacgaaactt
      361 tcggcattgt tagtgaaggg agcttcatgc ctgatgccag cgagtggaaa aaagtattgc
      421 tgccttggac ggttcggggt tttgctgatg acagtaaatt taaagcattc aacaaagaaa
      481 aaaacaacga caacaagcca aaatacagtc aaaaataccg cagccgcgac agcagcaagc
      541 acgagcgcga tttgggcgac atcgtcaaca gccccatcgt ggcggtcggc gggtatttgg
      601 ctacttccgc caacgacggg atggtgcata tcttcaaaaa aggcaacggc ggggacgagc
      661 gcaactatag tctgaagctc agttatatcc cgggcacgat gccgcgcaag gatattcaaa
      721 gccaagaatc cacccttgcc aaagagctgc gcgcctttgc cgaaaaaggc tatgtgggcg
      781 accgctacgg cgtggacggc ggctttgtct tgcgccgcat tacagatgac caagacaagc
      841 aaaaacactt ctttatgttc ggcgcaatgg gctttggcgg cagaggcgca tacgccttgg
      901 atttaagcaa aatcgacaac agcaacccgg ccggcgtttc catgtttgat gtcaaaaacg
      961 acaatggcgt gaaattaggc tacaccgtcg gtacgccgca aatcggcaaa acccacaacg
     1021 gcaaatacgc cgccttcctc gcctccggtt atgcgactaa agacattaac aacggcgaga
     1081 ataaaaccgc gctgtatgtg tatgatttgg aaaacaacaa cggtacgccg attgcaaaaa
     1141 tcgaagtgaa ggacggcaag ggcgggcttt cgtcccccac gctggtggat aaagatttgg
     1201 acggcacgat cgatatcgcc tatgccggcg atcgcagcgg gaaaatgtac cgctttgatt
     1261 tgagcggcaa cgacccgacc aaatggtctg tacgcactat ttttgaaggc gacaaaccga
     1321 ttacttccgc gcccgccatt tcccaactga aagacaaacg cgtggttatc ttcggcacgg
     1381 gcagcgattt gagtgaggaa gatgttgata aaaaggacga acaatatatt tacggtatct
     1441 ttgacgacga tacggagacg acgggtaatg taaaggtaga ccttaaaggt ttgggaggcg
     1501 ggctgctcga gcaacacctt actcaggaag ataaaacctt attcctgacc gattataagc
     1561 gatccgacgg atcgggcagt aagggctggg tagtgaaatt gcagcccgga cagcgcgtta
     1621 ccgtcaaacc gaccgtggta ttgcgtaccg cctttgtaac catccgcaaa tataaagaca
     1681 acggctgcgg cgcggaaacc gccattttgg gcatcaatac cgccgacggc ggcaagctga
     1741 ccaagaaaag cgcgcgcccg attgtgccgg cagccaattc gaaggtcgcg caatattccg
     1801 gcgataagaa aacttccagc ggcaaatcca tccctatagg ttgtatggaa aaagacaatg
     1861 gaatcgtctg cccgaacgga tatgtttacg acaaaccggt taatgtgcgt tatctggatg
     1921 aaaagaaaac agacggattt tcaacaacgg cagacggcga tgcgggcggc agcggaacat
     1981 tcaaagaggg taaaaaaccc gcccgcaata accggtgctt ctccggaaaa ggtgtgcgca
     2041 ccctgctgat gaacgatttg gacagcttgg acattaccgg cccgacgtgc ggtatgaaac
     2101 gaatcagctg gcgtgaagtc ttctactgat ttgcacgcga aaatgccgtc cgaaaggttt
     2161 tcggacggca ttttttgcgt ttttcgggag gggcgggttc gtaaaaggcg ggctataggg
     2221 taggcttcat ctcgccaatc tcactgaatc catcaatttc cacaattcaa ttaaataccg
     2281 tcaaaccga
//
LOCUS       DAWKQB010000199         2287 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.200, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000199 DAWKQB010000000
VERSION     DAWKQB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.200"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="WJL14_001983"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="WJL14_001983"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ7656997.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJL14_001984"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="WJL14_001984"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ7656998.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQH
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLVGATTRAGMLTNPLRDRFGIVSRLEFYENRDLATIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDVQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="WJL14_001985"
     CDS             complement(1884..2192)
                     /locus_tag="WJL14_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7656999.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQVRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          467 a          542 c          731 g          547 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat gagatagggt tcgataacgt cttcgatggt gtctgttgat
      901 tcgccgatgg cggcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga ggaatttcct gtccatcacg tccagcccct gcacgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc ggcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agttgcagga gttgtgccga acggctgacg
     1201 atggtggcaa ggtcgcggtt ttcgtaaaac tcgaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgacaagcgt gaagggcggc
     1321 aggtcgattt taacggaacg ggcggcgggc ccttcgccta tcataatgtc gagccggtag
     1381 tcttcaagcg cggggtagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 ataaacaata cgtcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gccggcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttacccag cccgggcgga ccgaaaagca agacgtggtc gagtgcttcg
     1621 ccgcgctttt tggcggcttg gatgaaaatg gcgagctgtt ctttggcttt atgctgcccg
     1681 atatagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacaat gcgttgcggt tgagcggcag tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtct gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttacccg cgcgtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgt gggcagatgc ctgtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt gataggcgtt ggggtcgaag cggacttgct gcatcaccgc ctgcgccaac
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa taggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggcg gaatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaact
//
LOCUS       DAWKQB010000200         2257 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.201, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000200 DAWKQB010000000
VERSION     DAWKQB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.201"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..567)
                     /locus_tag="WJL14_001986"
     CDS             complement(<1..567)
                     /locus_tag="WJL14_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ7657000.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGSVFNNAAQTWNHTFYWLGFTPKGQGKPVGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAK"
     gene            730..2136
                     /gene="dnaB"
                     /locus_tag="WJL14_001987"
     CDS             730..2136
                     /gene="dnaB"
                     /locus_tag="WJL14_001987"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ7657001.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPMFIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWMGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          537 a          585 c          598 g          537 t
ORIGIN      
        1 tttggcgact tcgtcccagt tgacgatttc ccagaaacct ttcaggtagt tgggacggct
       61 gttgcggtag tcgatgtaat aggcgtgttc ccacacgtcg caggtcagca gcggcgtgtt
      121 ttcagtggtc agcggagttg ctgcgttgga agtggaaacc aaatccaatc cgccggcagg
      181 ggtttttacc agccacgccc aaccggagcc gaaagtaccg gcagcgcagg cattgaacgc
      241 ttcttggaat ttctcgaagc tgccccattt cgcgtcgatg gcggcggcca gttcgccgac
      301 aggtttgcct tgacctttgg gcgtgaaacc cagccagtag aaggtgtggt tccaagtttg
      361 tgccgcgttg ttgaacacgc tgcccgaaga ttttttcaca atctcttcca aaggcaggtt
      421 ttcaaattcg gtgcctttga tttgattgtt caggttggtg atgtaggttt gatggtgttt
      481 gccgtagtgg aattccaaag tctctttgct cagatgcggg gacaatgcgt ccagttcata
      541 aggcagttgc ggcagcttat gttccatttt gtgctcctaa tattgtttta aatgttgtat
      601 tttggcagtg ttgctgcaaa tagctcagca gcgcacatat tttacctgtt ttgagggatg
      661 gaaaccaatt aaacctgctt tacgctataa tagaagattg caatttcggc acgacagata
      721 ggatgtacca tgaacgatta cgccgctatg ccgtctgaag accgtgaggt cggcgcattg
      781 tcgctgcctc cacattcgat ggaggcagaa caatccgttt tgggcgggtt gatgctggaa
      841 aatccggctt gggacaggat tgccgatgtg gtttcgggag aggatttcta ccgccacgaa
      901 caccgcctga ttttccgatc cattgccaaa ttaatcaatg agagccgtcc cgccgatgtg
      961 attacggttc aggaagattt gcagcggaac gaagaattgg aagcggcagg cggattcgaa
     1021 tacctgatta cgctggcgca aaatactccg tctgccgcca acatccgccg ctacgccgaa
     1081 atcgtgcgcg agcgttccat catgcgccaa ctcgccgaag tggggacgga aatcgcccgc
     1141 agcgcataca atccgcaagg cagggacgcg gggcagcttt tggacgaggc ggaaaacaaa
     1201 gtattccaaa tcgccgaaag caccgccaaa tccaagcagg gctttttgga gatgcccgat
     1261 ttgctgaaag aagtcgtaca gcgcatcgat atgctctact cgcgcgacaa tcccgatgaa
     1321 gttaccggcg tgccgacagg gttcatcgac ctcgacaaaa aaacctcggg tctgcagccc
     1381 ggcgacctga ttatcgtcgc cggtcgtccg tctatgggta agaccgcctt ttccatcaat
     1441 attgccgaac acgttgccgt agaaggcaag ctgcccgttg ccgtcttctc aatggagatg
     1501 ggcggtgcgc aactggtcat gcgtatgctc ggctcggtcg gacgcttgga tcaaagcgtt
     1561 ttgaaaaccg gcaggctcga agacgaacac tggggtcgcc tgaacgaggc agtcgtcaaa
     1621 ctttccgacg cgcctatgtt tattgacgaa accccgggtc tgaccgcgct cgaactgcgc
     1681 gcccgtgccc gccgtctcgc ccgtcaattt aacaataagc ttggattgat tgtcatcgac
     1741 tacctgcaac tgatggcggg atccggccgt tccgacaacc gtgcttcgga gcttggcgaa
     1801 atttcccgct ccctcaaagc cctggcaaaa gagttgcaag tccccatcat cgccctgtcg
     1861 caattgagcc gtaccgtcga atcgcgtacc gacaaacgcc cgatgatgtc cgacctccgc
     1921 gaatccggtg ctatcgagca agatgccgac ctgattatgt tcatgtaccg cgacgaatac
     1981 tacaaccaag actcgcccat gaaaggcctt gccgaatgta tcatcggcaa acaccgcaac
     2041 ggtcccgtcg gtaaaatctt cctcacatgg atgggacaat tcaccaaatt cgacaatgct
     2101 gcctatattc ccgaggaggc aaagatagag gattaaatgg ctgtataaaa atttattagg
     2161 cgaaatcagg caaaatcgtt taaaatcatg cttagagatt gccctaaaaa ataaaacgcg
     2221 gtcttgaggc atttttgcat tcagcccgca tataatt
//
LOCUS       DAWKQB010000201         2225 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.202, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000201 DAWKQB010000000
VERSION     DAWKQB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.202"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            108..1919
                     /gene="typA"
                     /locus_tag="WJL14_001988"
     CDS             108..1919
                     /gene="typA"
                     /locus_tag="WJL14_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ7657002.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          568 a          626 c          582 g          449 t
ORIGIN      
        1 gtctgaaagg caagggcttc agacggcatc ttcatttccc aaatactgtc cggtaaagcg
       61 tggtataatg cgtagttatc tcttacagtt tttgaaaaac attaattatg aaacaaatcc
      121 gcaacatcgc catcatcgcc cacgtcgacc acggcaaaac cacattggtc gaccaactgc
      181 tgcgccaatc cggcacattc cgcgccaacc agcaggttga cgagcgcgtg atggacagca
      241 acgaccttga aaaagaacgc ggcatcacca tcctcgccaa aaacaccgcc atcgattacg
      301 aaggctacca catcaatatc gtcgacacgc cgggacacgc cgacttcggc ggcgaagtag
      361 agcgagtttt ggggatggtg gactgcgtcg tcttgttggt ggacgcgcag gaaggcccga
      421 tgccgcaaac ccgtttcgtg accaaaaaag ccttggcttt ggggctgaaa ccgattgtcg
      481 tcatcaataa aatcgacaaa ccgtccgccc gtccgagctg ggtcatcgac caaactttcg
      541 agctgttcga caacttgggc gcgaccgacg agcagttgga tttcccgatt gtttatgctt
      601 ccggtctgtc cggtttcgcc aaattggaag aaaccgacga gagcaacgac atgcgtccgc
      661 tgttcgatac tatcttaaaa tatacgcctg caccgagcgg cagcgcggac gaaacgctgc
      721 aactgcaaat ttcccaactc gactacgaca actacaccgg ccgcctcggt atcggtcgta
      781 tcttgaacgg acgtatcaag cccggtcaag ttgttgccgt catgaaccac gatcaacaaa
      841 tcgcccaagg ccgcatcaac cagcttttgg gtttcaaagg tttagaacgc gtgccgcttg
      901 aagaagccga agccggcgac atcgtgatta tttccggtat tgaagacatc ggcatcggcg
      961 taaccatcac cgacaaagac aaccccaaag gcctgccgat gttgagcgtg gacgaaccga
     1021 cgctgacgat ggactttatg gtcaacacca gcccgttggc aggtacggaa ggcaaattcg
     1081 taaccagccg ccaaatccgc gaccgcctgc aaaaagaatt gctgaccaac gtcgccctgc
     1141 gcgtggaaga taccgccgat gccgacgtgt tccgcgtatc cgggcgcggc gagctgcacc
     1201 tgaccatttt gctggaaaac atgcgccgcg aaggctacga actcgccgtc ggcaaaccgc
     1261 gcgtcgtgta ccgcgacatc gacggtcaaa aatgcgaacc gtatgaaaac ctgaccgtgg
     1321 acgtacccga cgacaaccaa ggcgcggtaa tggaagaact cggccgccgc cgtggcgaac
     1381 tgactaatat ggaaagcgac ggcaacggac gcacccgcct cgaataccat attccagcgc
     1441 gcggcttgat cggcttccaa ggcgaattta tgaccctgac gcgcggggtc gggctgatga
     1501 gccacgtgtt cgacgattac gcgcccgtca aacccgatat gcctggccgc cacaacggcg
     1561 tgctggtgtc ccaagagcag ggcgaggcag tcgcttacgc cttgtggaat ctggaagacc
     1621 gcggccgtat gttcgtatcg cccaacgaca aaatctacga aggtatgatt atcggcatcc
     1681 acagtcgcga caacgatttg gtggtcaacc cgctcaaagg caaaaaactt accaacatcc
     1741 gtgccagcgg taccgacgaa gccgttcgcc tgaccacgcc gattaagctg acgctggaag
     1801 gtgcggtcga gtttatcgac gatgatgagc tggtagaaat cacgccgcaa tccatccgtc
     1861 tgcgcaagcg ttacttgagc gaattggaac gccgccgcca tttcaaaaag ctagattgat
     1921 gtttactgga ttaatgttta aatgatacgc gatgccgtct gaaaaatttc agacggcatt
     1981 ttttattcgg acgggctttg cggcttcttg aagctgtttc agacggcgtt tttcctaccc
     2041 aatcaagaaa ctgccgccat ttttccagcg gcatatcgcc ccgtcgcgtt tcggtatcgg
     2101 gttcggcttc ccggcagcat gtcgaacaat gccgtttgaa ggaatacgcc ttttgagtcc
     2161 ttacaagctt aggaagagga tatagtggat taacaaaaat caggacaagg cgacgaagcc
     2221 gcaga
//
LOCUS       DAWKQB010000202         2190 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.203, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000202 DAWKQB010000000
VERSION     DAWKQB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.203"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            345..2123
                     /locus_tag="WJL14_001989"
     CDS             345..2123
                     /locus_tag="WJL14_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ7657003.1"
                     /translation="MNKIYRIIWNSALNAWVAVSELTRNHTKRASATVKTAVLATLLF
                     ATVQANATDEDEEEELESVQRSVVGSIQASMEGSGELETISLSMTNDSKEFVDPYIVV
                     TLKAGDNLKIKQNTNENTNASSFTYSLKKDLTGLINVETEKLSFGANGKKVNIISDTK
                     GLNFAKETAGTNGDTTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLN
                     VGWNIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGA
                     KTSVIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTG
                     QADKFETVTSGTNVTFASGKGTTATVSKDDQGNITVMYDVNVGDALNVNQLQNSGWNL
                     DSKAVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEISRNGKNIDIATSMAPQFSSV
                     SLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLN
                     NRIDNVDGNARAGIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGG
                     NWIIKGTASGNSRGHFGASASVGYQW"
BASE COUNT          665 a          533 c          555 g          437 t
ORIGIN      
        1 cacaggaatc tatcagaaaa aacagaaacc cccaccgccg tcattcccgc gcaggcggga
       61 atccagcaac acaacgcggc aggactttat cggaaaaaac agaaacccca ccgaccgtca
      121 ttcccgcaaa agcgggaatc cagacccgtc ggcacggaaa cttaccggat aaaacagttt
      181 ccttagattc cacgtcccag attcccgcct tcgcgggaat gacgagattt taagttgggg
      241 gaatttatca gaaaaccccc aacccccaaa aaccgggcgg atgccgcacc atccgccccc
      301 aaaccccgat ttaaccattc aaacaaacca aaagaaaaaa caaaatgaac aaaatatacc
      361 gcatcatttg gaatagtgcc ctcaatgcct gggtcgccgt atccgagctc acacgcaacc
      421 acaccaaacg cgcctccgca accgtgaaga ccgccgtatt ggcgacactg ttgtttgcaa
      481 cggttcaggc gaatgctacc gatgaagatg aagaagaaga gttagaatcc gtacaacgct
      541 ctgtcgtagg gagcattcaa gccagtatgg aaggcagcgg cgaattggaa acgatatcat
      601 tatcaatgac taacgacagc aaggaatttg tagacccata catagtagtt accctcaaag
      661 ccggcgacaa cctgaaaatc aaacaaaaca ccaatgaaaa caccaatgcc agtagcttca
      721 cctactcgct gaaaaaagac ctcacaggcc tgatcaatgt tgaaactgaa aaattatcgt
      781 ttggcgcaaa cggcaagaaa gtcaacatca taagcgacac caaaggcttg aatttcgcga
      841 aagaaacggc tgggacgaac ggcgacacca cggttcatct gaacggtatc ggttcgactt
      901 tgaccgatac gcttgcgggt tcttctgctt ctcacgttga tgcgggtaac caaagtacac
      961 attacactcg tgcagcaagt attaaggatg tgttgaatgt gggttggaat attaagggtg
     1021 ttaaaactgg ctcaacaact ggtcaatcag aaaatgtcga tttcgtccgc acttacgaca
     1081 cagtcgagtt cttgagcgca gatacgaaaa caacgactgt taatgtggaa agcaaagaca
     1141 acggcaagag aaccgaagtt aaaatcggtg cgaagacttc tgttattaaa gaaaaagacg
     1201 gtaagttggt tactggtaaa ggcaaaggcg agaatggttc ttctacagac gaaggcgaag
     1261 gcttagtgac tgcaaaagaa gtgattgatg cagtaaacaa ggctggttgg agaatgaaaa
     1321 caacaaccgc taatggtcaa acaggtcaag ctgacaagtt tgaaaccgtt acatcaggca
     1381 caaatgtaac ctttgctagt ggtaaaggta caactgcgac tgtaagtaaa gatgatcaag
     1441 gcaacatcac tgttatgtat gatgtaaatg tcggcgatgc cctaaacgtc aatcagctgc
     1501 aaaacagcgg ttggaatttg gattccaaag cggttgcagg ttcttcgggc aaagtcatca
     1561 gcggcaatgt ttcgccgagc aagggaaaga tggatgaaac cgtcaacatt aatgccggca
     1621 acaacatcga gattagccgc aacggtaaaa atatcgacat cgccacttcg atggcgccgc
     1681 agttttccag cgtttcgctc ggcgcggggg cagatgcgcc cactttaagc gtggatgacg
     1741 agggcgcgtt gaatgtcggc agcaaggatg ccaacaaacc cgtccgcatt accaatgtcg
     1801 ccccgggcgt taaagagggg gatgttacaa acgtcgcaca acttaaaggc gtggcgcaaa
     1861 acttgaacaa ccgcatcgac aatgtggacg gcaacgcgcg tgcgggcatc gcccaagcga
     1921 ttgcaaccgc aggtctggtt caggcgtatc tgcccggcaa gagtatgatg gcgatcggcg
     1981 gcggcactta tcgcggcgaa gccggttacg ccatcggcta ctccagtatt tccgacggcg
     2041 gaaattggat tatcaaaggc acggcttccg gcaattcgcg cggccatttc ggtgcttccg
     2101 catctgtcgg ttatcagtgg taagggcttt ttcgcctgtc tgctgttggg acaggcggaa
     2161 ggtttgaagg gaagggtggc gatttgccgc
//
LOCUS       DAWKQB010000203         2142 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.204, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000203 DAWKQB010000000
VERSION     DAWKQB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.204"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            30..463
                     /locus_tag="WJL14_001990"
                     /pseudo
     CDS             30..463
                     /locus_tag="WJL14_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            559..1182
                     /gene="rsmG"
                     /locus_tag="WJL14_001991"
     CDS             559..1182
                     /gene="rsmG"
                     /locus_tag="WJL14_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ7657004.1"
                     /translation="MERKERLRAGIAALGLDISAAAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMVVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTAHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            1281..2054
                     /locus_tag="WJL14_001992"
     CDS             1281..2054
                     /locus_tag="WJL14_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ7657005.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGAKAYLALADELMARVSGK"
BASE COUNT          421 a          490 c          737 g          494 t
ORIGIN      
        1 gcggtctgct tcggaagagg gggacggcga tgtttgtgaa cgagaaagat ccttatgcgg
       61 ctctgtttgc gggactggtg tttttgacgc tgccgtttgc gttggcggtg catgatgcct
      121 ttgcgcttgc gttcggacgg gcggggctgc tggtgtcggt gtcggacggc ggattcggct
      181 ggcatggcgg ttgggacggc actgtttggt ttgtgttcgg tgtgtttgcg tttttgaatg
      241 tggttgtgtc ggcgggcctg acgaaactgg cgtacaaaaa gatgatgcgg cggcattcgc
      301 gtggattttc gagctgctgt ttggcagtgc cgccttgggc gggctgcgtg gggaggcggt
      361 gttggaatat gcgtttgccg tgtggctggt gtcgatgctg acactgccca aacgcttgac
      421 gcgcgcgccg gtgcagccgg tggtgtttca caggaaaaaa taggacggaa cgggggatgc
      481 cgtctgaaac ccgacacgcg gtttcagacg gcatgttttt ccgttaacat tacgcctgaa
      541 catggacagg atagggatat ggaacgcaaa gaacgcctgc gtgcaggcat cgccgcattg
      601 ggtttggata tttccgcagc ggcgcaggac aggcttttgg cctatgtgga tttgttgaaa
      661 aagtggaaca aaacctacaa tctgaccgcc ctgcgcgacg aggaaaaaat ggttgtccat
      721 catcttttgg acagcctgac gctgctgccc catatcgagg gtgtgcaaac gatgctggat
      781 gtcggttcgg gcggcggtca gcccggcatt ccggcggcgg tgtgccgtcc ggatgtgcaa
      841 ataacccttt tggatgcgaa tacgaagaaa acggcttttt tacagcaggc ggttatcgag
      901 ttggggttgg acaatgtgcg cgtggtatcc ggacgcgtgg aggcggtttc ggacgtgcgt
      961 gccgacgtgg ttaccagccg tgcgtttgca gaactggcgg actttgtatc gtggacggcg
     1021 catctgttga aagacggcgg ctactgggcg gcgatgaagg gcgtatatcc gcagggggaa
     1081 atcggccgcc tgccgcagga tgtgtgcgtt gaaaaagtcc aaaggctcga cgtgccaggc
     1141 ttggatgcgg aacgccatat cgtcatcctg agcaagcgtt gagcgcgctt cagacggcat
     1201 gaataccttt tttgtgcgga taaaggtaaa attccgcact gtttttcttt tttcaacatc
     1261 agacgggaca cgggcgggac atgagtgcga acatccttgc catcgccaat cagaagggcg
     1321 gtgtgggcaa aacgacgacg acggtaaatt tggcggcttc gctggcatcg cgcggcaaac
     1381 gcgtgctggt ggtcgatttg gatccgcagg gcaatgcgac gacgggcagc ggcatcgaca
     1441 aggcgagttt gcagtccggc gtttatcagg tcttattggg cgatgcggac gtgaaatcgg
     1501 cggcggtacg cagcaaagag ggcggatacg gcgtgttggg tgcgaaccgc gcgctggccg
     1561 gcgcggaaat cgagctggtg caggaaatcg cccgggaagt gcgtttgaaa aacgcgctca
     1621 aggcagtggc ggaagattac gactttatcc tgatcgactg cccgccttcg ctgacgctgt
     1681 tgacgcttaa cggcttggtg gcggcaggcg gcgtgattgt gccgatgttg tgcgaatatt
     1741 acgcgctgga agggatttcc gatttgattg cgaccgtgcg caaaatccgt caggcggtca
     1801 atcccgattt ggatatcacg ggcatcgtgc gtacgatgta cgacagccgc agcaggctag
     1861 ttgccgaagt cagcgaacag ttgcgcagcc atttcgggga tttgctgttt gaaaccgtca
     1921 tcccgcgcaa tatccgcctt gcggaagcgc cgagccacgg tatgccggtg atggcttatg
     1981 atgcgcaggc aaagggtgcc aaggcgtatc ttgccttggc ggacgagctg atggcgaggg
     2041 tgtcggggaa ataggtcaat ccaaatcggg ctgcccgtgc ctttatgctg tttggccggg
     2101 tgcgttaata tggcggatta aaataaaaat acttatatcg tc
//
LOCUS       DAWKQB010000204         1944 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.205, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000204 DAWKQB010000000
VERSION     DAWKQB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.205"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            242..1699
                     /locus_tag="WJL14_001993"
     CDS             242..1699
                     /locus_tag="WJL14_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ7657006.1"
                     /translation="MSRHPARTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIVYFTRLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          431 a          581 c          448 g          484 t
ORIGIN      
        1 gccctatcgg ataagtcgct tgactgaaaa ggctttaccc gcgccgtatc ggaacacgtc
       61 ctctaaaata caatccgttg aattgaaaca aatataaaaa cattcgaccg caaaaacggc
      121 agcgcatcgt ttgacaaaga ttgaaaatat cggttaaaaa ccgattttca tacaaaaaac
      181 accgctgccg tccgcatccg tttcagacgg tattgagaga aaatctttta ggagaacctt
      241 tatgtcccgg catcccgccc gcaccggaga aaaaacattc ttcggccacc ccttccagct
      301 ttccaccctc ttccatatcg aattgtggga acgtttttca ttttacggaa tgcagggcat
      361 cctgctgatt tacctctact acaccgccga caaaggcggc ttgggcatag acaaaaccct
      421 cgccggcggc attgtcggcg catacagcgg cagcgtgtac ctgtccacca ttttgggcgc
      481 gtggtttgcc gaccgcgtat ggggtgcgga aaagaccctc ttcctctcgg gcatcgtcgt
      541 gatgctcgga cacatcgtcc ttgccgccgc cccgggcctg tacggccttt taatcgggct
      601 gatattcatc gcattgggca gcggcggtgt gaaatctacg gccagttcta tggtgggcgc
      661 attatacgaa caggacgaaa tgcgcccgct gcgcgatgcg ggattttcca ttttctacat
      721 cgccatcaat atcggcggct tcctaggccc gctgctgacc ggcctgctgc aggaaaacat
      781 cggtttccat tatggtttcg gcgcggcggc ggtcggtatg gcattcggct tgtggcgtta
      841 ttccttggga cgtaaaaacc tgccccaccc caccgtcccc catccgcttt caaaaggaca
      901 gggcaaaact gcggccgccg tcggcatcgc cctcatcgcc gcacttgcaa ccgccatcaa
      961 aaccgggctt gtcaacctcg acaatttctc cggcatcctc ctatctaccg tcatccttgc
     1021 cgtcatcgtc tattttaccc ggctgctgac caacccccgc gtcagttccg acaacaagcg
     1081 gcacatcatc gcctacatcc cgcttttcct gaccatctgt atgttttggg cggtctggtt
     1141 tcagatttac accgtggcaa ccgtctattt cgacgaaacc gtcaaccgca ccatcggttc
     1201 gtttaccgta cccgtcgctt ggaaagattc tatgcaaagc ctgtgggtca tcctgttttc
     1261 cggacttatg gcggcaatgt ggacaaaaat ggggcgcaaa cagcccaaaa ccccgctgaa
     1321 attcgctatg gcggtatttg ttaccggcgc gtcgtttttg ggattcgtcc cctttatttc
     1381 cgccggcacg ccgatgccta ttgcggtttt cgcactgatt gtccttgcca tcacgatagg
     1441 cgaactgatg atttccccga ttgcgctgtc catctccacc aaaatcgcac cgcctttatt
     1501 caaaacccaa atggtcgccc ttaatttcct tgccttttca ttaggcttca ctttgggcgg
     1561 cgtattgttt gaaaaaggct atcaggcggg cgacgaaatc ggcttctatc ggctgctgtt
     1621 ctacatcggc gcagccacag gcttcctgct gctcctgctc gtccccaaat tgaacaaaat
     1681 gctcgaaggc acagactaag tcccgccccg atgccgtcta aacccttcag acggcatttt
     1741 tccgcataat gaaaccaaac cgtttccacc cgacaggaca ggctcccccc ccaaccggaa
     1801 ggcagcctgc cgattgtcat ttaaatatct gactgattcc gtctgtttgc aagggaaata
     1861 gccgtttatt tccgcttggg cggaaaccgg ttctattgag taaaaggcat tttatccgac
     1921 taaattagtg ctgcatcaac ggaa
//
LOCUS       DAWKQB010000205         1891 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.206, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000205 DAWKQB010000000
VERSION     DAWKQB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.206"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(660..1718)
                     /gene="alr"
                     /locus_tag="WJL14_001994"
     CDS             complement(660..1718)
                     /gene="alr"
                     /locus_tag="WJL14_001994"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ7657007.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASVYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
BASE COUNT          474 a          474 c          421 g          522 t
ORIGIN      
        1 agtttatcct agttatccag gacagcccct aaatttaaac cagtacagcg ttgccgtact
       61 atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctataaaaaa
      121 tccctgtgtc cttatttctg ctttcttacg tcattctttt aaaggtggga atctggaaac
      181 caaaaaacaa caggaattta tccgaaacaa caaaacctat ccgccggcat ccccgcgaaa
      241 gcgggaatct agtttgttgt tcaaataaat tctgaaactt taattttgtc attcccgcgc
      301 aggcgggaat ctagaaatga aaaacgacga gaatttatca gaaatgacgg aaactcaaaa
      361 aaccggattc ccgcctgcgc gggaatgacg aaattttagg tttctgattt tgattttccg
      421 tttttgcggg aaggatgggg tttcagattg tgggcattat cagtattttc tatttggcaa
      481 tgtcttttat aatggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtacgat
      541 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttaatccact atactgattt
      601 tgtcaaaacg catttatgcc gtctgaaagg cttcagacgg catttttgtt tggacttgat
      661 tactcgatat aagtgaattt tgcacgtttg atattgcaca tcaattcgta agggatggtt
      721 ccggccgctt cggcaacggt attgatatta accgtatcgc cccacagttc gacctcgtgt
      781 cccaaacctt cttgcgaagc gtccagctcg atggtcatca tatccataga gaccctgccg
      841 atgacccggg tcaatttacc gtcgacggcg acgggggaat tgcttggggc gcggcgcgga
      901 taaccgtccg cataaccgca ggcaatcagg ccgacgcgcg tagatttact ggtataaaat
      961 gttgcgccat aaccgatagg ggagtgcggc tgtaaaacgc gttcgccgaa aatacgggtg
     1021 gaaagcctca tcacgggttt cagcctgtca tcgctgcctc cgaacgggga aatgccgtac
     1081 aacgccaagc cggctcgccc ccagtccctg cgtgcttcgg gaacattcaa aatagcggcg
     1141 gagttggcaa ggctttcttc gccttccagc ccttccgtac ccaaatcgaa tgcttccatc
     1201 tgtatttccg tcataccgct ttcgggttcg tccgcacagg agaaatgcga gaatttgaca
     1261 atactgtcca catattccga ctgcttcaat gccgcatata ccgaagcgta atcatgaggg
     1321 aaaaatccgg tacggtgcat tcccgaatcc attttcaacc agaccttgac aggttttttc
     1381 caatggcggg aaagcaaagc ctcaagttgc cattggtttc cgactgccgg ccagagcgag
     1441 tattgttcga ccgcttcgta ctctgatgct tcaaatacgc cttccaaaag gacaatcgga
     1501 tgggtaatgc cactctcccg tagcctgatt ccctcgtcga ttgtcgccac ggcaaagccg
     1561 tctgccaagt ctgccagcgc gaaagcacat ctgaccgcac cgtgtccgta tgcgtcggcc
     1621 ttcactaccg ccaacagttt gcctccgtgc atttccttca aaatccgata attgtgccta
     1681 aggttgccca acctgatctg cacgttcaaa gggcgcataa attcattcct taccaaacaa
     1741 gtgtttatta gcaaaattaa gaaatcaatt agggaattat aatccaacgg caaggctttt
     1801 aaaatattaa agacccttgt atcttcaagc cgaagcagca gtttttaggt aggaaggcgg
     1861 tttgcttttt ctgatataat cgctttccca a
//
LOCUS       DAWKQB010000206         1871 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.207, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000206 DAWKQB010000000
VERSION     DAWKQB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.207"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..761)
                     /locus_tag="WJL14_001995"
     CDS             complement(<1..761)
                     /locus_tag="WJL14_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ7657008.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHV"
     gene            complement(1318..1767)
                     /locus_tag="WJL14_001996"
     CDS             complement(1318..1767)
                     /locus_tag="WJL14_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657009.1"
                     /translation="MQPGSKNDEKNKITLNEFNNAIVTIFSDENVVRAIGTINTMHGE
                     YLTRSHKIDQQKFLSDGERLSSKAKDFQHSAEKTQRALKEGIGRAASKFHANSKEAFV
                     ARKMQSDLQKKLNASNYNKQQYARQAVTALENASKAENKGNIAFYLN"
BASE COUNT          617 a          399 c          228 g          627 t
ORIGIN      
        1 acgtggtatt tgtcgctgcg gttaagattt aagaaatcct caaaccatct ccaagctttt
       61 tcagctccta atgatcccca ccaagctcct gcgccacctc caagtatagc tccaactact
      121 agccaacctg cgccaccagt aagtaaacca aaagccccaa cacccaaagc cgcaccccat
      181 tggacgccca ttagcccacc caaaaaagaa gctgatgaga gaccaacatc actccaattt
      241 ccttctccat ccaaggcttt ccaccaagca cttcccatac tcaaagttcc catggcaaca
      301 gttgccccat aagtaaactt acccaaattc gctgccataa cttttgattt tgaagcattc
      361 tctaacgctg ttgccgcacg tcttgcatat tgctgcttgt tatagttgct agcattcaat
      421 gctttttgca aatcactttg tacttttctt gcaacaaatg cctcctttga attaccacga
      481 aatttactag cagctcttcc aattccttct tttaagcttt tttgtgcctc ttctgctgtg
      541 cgttggaatt ctcttgcctt agcagacaga cgctcaccct cccttaaaaa ttctttttga
      601 tctatcttgt gcgaacgggt caaatattct tcgtacattg agttaatcgt accaatcgcc
      661 ctaaccacat tttcatcaga aaatatggaa ataatagcat ccctatacgc acctagtgta
      721 atattgtttc tattattagt tatagcatta ttcgaataca taatagcacc tccaaattta
      781 gaactgaata cgtttatatt ctctgtgtaa taatatcaaa taattaatta atggataagt
      841 aagtccgaaa aaataacaac taaaataagc aatatactga ttctctcctt tattaattag
      901 atatattgaa aaacaaataa aaaataaaca gaaaacattc atgtgaaaaa gccacctcat
      961 attcttattt ataagtttaa aataactagg cattgtatat ttaaaaatcg gcacatccaa
     1021 cggaaacaaa ccacataaaa atttttcttt attctttatt aaagacatag ttttattttt
     1081 taaaattagt ccaaataata tactaacaat tactattaaa acaataagca cagcgtaatt
     1141 ttttataaaa ttcattaaaa taataacgaa aatactaaat agattacata ctataaattg
     1201 aaatataaaa tattcagtat ttaatccatt ttcaaccgcg agttttttaa aatatccttg
     1261 ctcgaaaaaa ttaaaaaaat ccattaatac tttcctctca agaaaactaa ttttagttta
     1321 gttaagataa aatgcaatat tacctttatt ttctgctttt gaagcatttt ccaaagctgt
     1381 gactgcttgt cttgcatatt gttgcttgtt atagttacta gcatttaact tcttttgcaa
     1441 atcactttgc atttttcttg caacaaatgc ctccttcgaa ttggcatgaa atttactagc
     1501 agcccttcca atcccttctt ttaaagccct ttgagttttt tctgctgagt gctgaaaatc
     1561 tttcgcttta ctagacaacc gctcaccatc actcaaaaat ttttgttgat ctattttatg
     1621 cgaacgagtt aaatactccc catgcattgt gttaattgta ccgattgccc taaccacatt
     1681 ttcatcagag aaaatagtta caatggcatt attgaattca tttagtgtaa tcttattttt
     1741 ttcatcattc ttagaaccgg gttgcatgac aacaccttcc atcaactaaa atattatatt
     1801 attaagagac tacaacttag atacgtttat aatctctgga taaaaaaacg taataagtgt
     1861 ttgctggata a
//
LOCUS       DAWKQB010000207         1827 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.208, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000207 DAWKQB010000000
VERSION     DAWKQB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.208"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            323..1768
                     /gene="zwf"
                     /locus_tag="WJL14_001997"
     CDS             323..1768
                     /gene="zwf"
                     /locus_tag="WJL14_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ7657010.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTAGFLAKAETNSKIHIKQNFSDEAWASFVKRIEYLKVDVTEAADFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQACENLAAIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT          503 a          425 c          479 g          420 t
ORIGIN      
        1 aaggtatacc gtctgaaagt gggaagcggg tgttattata gtacaaaatt tgtaattttg
       61 ttacaaaaaa atcagacatc gaaaagaaat ttgatttaaa tcgcagaatc tttcgattgt
      121 tgtaacggaa taggggaaac ctgcccgatt tttggactag gtcaaataaa ttgtaaaaat
      181 gggaatagac aaaaagtgta gcggtgtgta gtattactac tcattatgcc gcctgccttt
      241 gtttccgata ttgtgatgca gcgtgatggg gaaaacaggt ttcaaacggc atcgaaaaat
      301 tctttcctga cgagataatg cgatgagtac acaaacaaat tttgatttgg tgttgttcgg
      361 tgcgaccggc gatttggcaa tgcgcaaact tttgccctgc ctgtatcagg cgcacgtggc
      421 cggtttgctt catcccgaag gtcgtatttt gggtgtgagc cgcagcgaat tggataccgc
      481 cggttttttg gcaaaagcgg aaacgaattc taaaatccat atcaaacaaa atttttcaga
      541 tgaagcgtgg gcttcgttcg tcaaacgcat cgaatacctg aaggttgatg ttaccgaagc
      601 agccgatttt gccgctttgg gcgatttggt caaagcgcgc aaagaaaccg acaacgtcgt
      661 catctatctg tccaccgcgc ccaaattctt cgcgcaagcc tgtgaaaacc ttgctgcgat
      721 cggtttgaat gccgacaatg tgcgcgtagt tttggaaaaa ccgttgggta cggatttggc
      781 ttcttcccag caaatcaata ccgatgttgc gcgttatttc aaagaagggc aaatttaccg
      841 catcgaccac taccttggca aagaaagcct gcaaaacctg cttgcgctgc gttttgccaa
      901 tgtaatgttc gagccgctgt ggaacaataa atacatcgaa agcgtgcagt tgaccatcgc
      961 cgaacagctc ggtgtggaag agcgcggcga gttttacgac attactggcg ccttgcgcga
     1021 tatggtgcaa aaccacttga tgcaaatgct gtgcatgact gcgatggaag cccccgccag
     1081 cttggatgcc gacgcggtgc gcgatgaaaa agtcaaagtc atcaagtcat tgaagccgct
     1141 gaccgtcgag tctgtcaatg aaaacgtcgt gcgcggacaa tataccgccg ccagaggcat
     1201 gaacggctat cttgaagaaa tcaacgttcc gcaagacagc tttaccgaaa cctacgtcgc
     1261 cattaaagcc gaaatcgaaa acgaacgctg gaagggcgtt cccttctacc tgcgtaccgg
     1321 caaacgcatg gcgggcaaag tggcggaaat cgttttgaac ttcaaagatt tgaacagcca
     1381 tatttttgaa ggcagccgca ccgcgcccaa ccggctcgtt atcgagttgc aaccatatga
     1441 atccgtgcgc ctctatacgc agatgaaaac cccgggggca ggaaataagg tcgaaaccgt
     1501 gccgctggca accgatttgg gcaaagcatt ggaaggccgc cgcgcggaag cttacgagcg
     1561 cctgctgctg gatgtgatta acggcaaact cgctttgttc aaccgccgcg acgaactcga
     1621 agccgcgtgg gaatatgtga tgccgatttt ggaaaactgg acaaataaca ccacgccgcc
     1681 gcacggctac ggcgcacact cgtgggggcc tgaagccgcg cgcgaactat tggcgcgcga
     1741 cggacacaag tggcacgaag agcagtaata caataatgcg ttcagacggc atggggtttg
     1801 aaatgccgtc tgaacgtaag taaagca
//
LOCUS       DAWKQB010000208         1816 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.209, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000208 DAWKQB010000000
VERSION     DAWKQB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.209"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(29..460)
                     /locus_tag="WJL14_001998"
     CDS             complement(29..460)
                     /locus_tag="WJL14_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657011.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTSCSLHET"
     gene            complement(476..>1816)
                     /locus_tag="WJL14_001999"
     CDS             complement(476..>1816)
                     /locus_tag="WJL14_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7657012.1"
                     /translation="VNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHKIAH
                     AIAGCAAAAANKGKCQDGAIGAAVGEIVGEALTNGKNPATLTAKEREQILAYSKLVAG
                     TVSGVVGGDVNAAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKY
                     QNVADKRLAASIAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFS
                     KSYTQADLALQSYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFV
                     KQNTPITNVKYPEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVK
                     SETQTDIEGITRIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAA
                     SQGYSKASKIAQNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          485 a          359 c          315 g          657 t
ORIGIN      
        1 gctggagcac acaccttacg catggatttt aggtttcatg caggctacag cttgtttgag
       61 aatatgtttt ttgtatttct ttacactttt tttgatattc agggtgagct attaagaaat
      121 cagatattgc aatattaaag taatgatatg ctaaattaat aggtataatt aattcttcag
      181 gattgaaatc atctccaact gaaaaataaa tagaaggagc tttatcaata tactcaaatc
      241 catttacctc ttcataatct ccaatccatc tggtagcatc tattttttca tattgagcac
      301 ctattactcc aaaatgtccg gtagttgtaa tatttttaaa acactctaaa agcgtatctt
      361 tagtttcaaa ttggtaaatt gtttcaaaaa aaatcgaaat aacaaaatgg ttgttattta
      421 tagatttttt atttaagttt agaaaaatat tatttttcat aatttttcct ttaaattatt
      481 ctgggtgaat gtttgtaatt cttcctgtat ttacatcaaa atatgatcta aatttgattc
      541 cgtcaaaggt ttctgagaat tgaatgacat tttttctttc cgatattgat ttagttcttt
      601 cattttgagc aattttagag gcttttgaat atccttgtga agcagcattt tgagccattt
      661 gaagtatttt atcatcagaa aattttttag gattataaac agtttttata cttgaaattt
      721 ccttaaatcc accatcaggt ttacctgtcc tgtctagtgt aggaatctca tatttaattc
      781 gggtaatgcc ttcaatatca gtttgggttt cagattttac gcgtcctcct cgtgaattta
      841 gttctgccat aaaattggtg cggttatggg ctcctttaat gccctgtttt tgactaaaac
      901 catcagcatt tgccagatgt ctttttaggt ttgtatcgaa actgatgcct tccgggtatt
      961 tgacattagt aataggtgta ttttgtttta caaaccctct tggtattgga atgaatctag
     1021 gattaactcc tgaaataagt gtataacctt ggtcggacat ccattcggat aaaggctttg
     1081 tattgcccga ttgaagccaa gatttagcag cagtattcaa atgataagac tgtaaagcca
     1141 aatctgcttg ggtgtaagat ttgctgaata atttatacca ttcatcatct ttaccaatta
     1201 gacctgcttc ccaagatgaa tgaaggcttc tactatcgat caaatgttgt tttctgattg
     1261 ttctacattc agtactacgg gatatatccg tacatattgc aatcgaagca gcaagtcttt
     1321 tatcagcaac attttgatac ttttttacag tattttttct gcacagttga ggattattct
     1381 gtttggcgca tgcagtcatt tcgttatcaa attctctacc ctctttgtcg ctaagctgat
     1441 tatttttcac cgctacctca gccgcattcg ccgccgcatt tacatcgccg ccgaccacac
     1501 cgcttaccgt accggcaacc agtttgctgt atgccaaaat ctgttcgcgt tctttagctg
     1561 tcaaagtggc aggatttttg ccgtttgtca aagcctcccc gactatctcg cccacagccg
     1621 caccgatcgc accatcctga cacttgccct tattcgccgc cgctgccgca cagcccgcta
     1681 tggcatgggc aatcttgtgg actatgtagt gctgatccaa ctgtttgatt ttactggctg
     1741 cttctccatg cgcggtattg accaaagccg caaggatatt cgcttccaga ttgtctttca
     1801 ggctgccgcc gttgac
//
LOCUS       DAWKQB010000209         1810 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.210, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000209 DAWKQB010000000
VERSION     DAWKQB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.210"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            1..1810
                     /gene="frpC"
                     /locus_tag="WJL14_002000"
                     /pseudo
     CDS             1..1810
                     /gene="frpC"
                     /locus_tag="WJL14_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
BASE COUNT          534 a          407 c          441 g          428 t
ORIGIN      
        1 tttgccaaac acggctacgc ggctttggcc gaattggatt caaacggcga caacatcatc
       61 aacgcggcag acgccgcatt ctaaaccctg cgtgtatggc aggatctcaa ccaggacggc
      121 atttcccaag ctaatgaatt gcgtaccctt gaagaattgg gtatccaatc tttggatctc
      181 acctataaag atgtaaataa aaatctcggt aacggtaaca ctttggctca gcaaggcagc
      241 tataccaaaa caaacggtac aaccgcaaaa atgggggatt tacttttagc agccgacaat
      301 ctgcacagcc gcttcaaaga caaagtggaa ctcactgccg aacaggcaaa agccgccaat
      361 cttgcgggca ttggtcgtct gcgcgatttg cgcgaagctg ccgcattgtc cggtgatttg
      421 gccaatatgc tgaaagctta ttctgccgcc gaaactaaag aagcacagtt ggcattgtta
      481 gataatttga ttcacaaatg ggcggaaacc gattcgaact ggggcaaaaa atcgccaatg
      541 cgactttcaa ccgattggac gcaaacggct aatgaaggta ttgcactgac accatcccaa
      601 gtagcacaac taaaaaagaa cgctttagtt tccctttctg ataaagctaa agcagctatt
      661 gacgccgccc gcgaccgcat tgccgtgctt gatgcctaca cggggcagga ttccagcacg
      721 ctttattaca tgagcgaaga agacgcgctt aatatcgtca aagtaaccaa cgatacatac
      781 gaccatctcg ccaaaaacat ctaccaaaac ctgttgttcc aaacccgttt gcagccatat
      841 ttgaatcaaa tcagtttcaa aatggaaaat gatacgttca ctttggattt tagtggtctt
      901 gttcaagcat ttaaccatgt caaagaaact aatccgcaaa aagcttttgt ggatttggcc
      961 gagatgcttg catatggcga acttcgttct tggtatgaag gtcgaagact aatggccgat
     1021 tatgtggagg aggcaaaaaa agcaggtaaa tttgaagatt accagaaagt gttgggtcag
     1081 gagaccgttg cattattagc taaaacatcg ggtacgcaag cagatgatat cctgcaaaat
     1141 gtaggctttg gtcataataa aaatgtttct ttatatggta atgacggtaa cgacactcta
     1201 atcggcggcg tcggtaatga ttacttggag ggcggcagcg gttcggatac ttatgtcttc
     1261 ggcaaaggct tcggtcagga tacggtctat aattacgact acgctaccgg acgcaaagac
     1321 atcatccgct ttaccgacgg tattacagcc gatatgctga cttttacccg agagggcaac
     1381 catcttctta tcaaggcaaa agacggcagt ggacaagtga ctgttcagtc ctatttccag
     1441 aacgatggct caggtgctta ccgtatcgat gagattcatt tcgataacgg caaagtactg
     1501 gatgttgcca ctgtcaaaga actggtacag caatccaccg acggttcgga cagattgtat
     1561 gcctaccaat ccggaagtac cttaaacggc ggattgggcg atgactatct gtacggtgcc
     1621 gacggggatg acctgctgaa tggtgatgca ggcaacgaca gtatctacag tggcaatggc
     1681 aatgatacgc tcgatggagg agaaggcaac gacgccctgt acggctataa tggtaacgat
     1741 gtactgaatg gtggcgaagg caatgatcat ttgaacggcg aagacggtaa cgacactctg
     1801 atcggcggtg
//
LOCUS       DAWKQB010000210         1742 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.211, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000210 DAWKQB010000000
VERSION     DAWKQB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.211"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(28..351)
                     /locus_tag="WJL14_002001"
     CDS             complement(28..351)
                     /locus_tag="WJL14_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ7657013.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(339..579)
                     /locus_tag="WJL14_002002"
                     /pseudo
     CDS             complement(339..579)
                     /locus_tag="WJL14_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
     gene            complement(702..1499)
                     /gene="truA"
                     /locus_tag="WJL14_002003"
     CDS             complement(702..1499)
                     /gene="truA"
                     /locus_tag="WJL14_002003"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ7657014.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQTALETALAQI
                     AGEFVATTVAGRTDTGVHATAQVVHFDTTAVRPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIEPMRRAAAYLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPKIPEWL"
BASE COUNT          393 a          409 c          499 g          441 t
ORIGIN      
        1 tgaacacaat ccattcagac tattcaatca ggcaaacatc tcctgcaata ctgcaaacag
       61 tttttcagcc gtactgttgt ctaaattgcc aagatgcttg accaatccgg ctttatccac
      121 agccctaatc tgttcgggca aaagcaaacc gtctttatcc tgaaagcgga cattgacgcg
      181 gaacggggca ggacggcttc cgctcgtcat gggaacgatc agcacagtct tgagatagtt
      241 gtgtatttca ggaggagaga ctacgacaca aggacgtgtc tttttgattt cgcttcctac
      301 ggtcgggtct aaggagacca gatagattcc gccgcgtact accatatcca ttctttatcc
      361 gcttcgtttt caatttcgga aaaaaaaatg ctcctgctcg gtttcgacaa gcattgcggc
      421 agcttctgcc catcccctgc gaacggtagg acagcttaaa ataatattgc ccttttcaac
      481 tgtaacagcc aagctgtcta ctgcccctat ttgacccaat aatgatttgg gcagaatcac
      541 gccttgcgag tttcccattt tgcgtatgtt gagaatcata tacgtaccct atttaccagt
      601 ttatgtaata acaatgttat taccttgatg aggtagtgtc aacattgaaa aagattgcct
      661 gacagtttgt ccgatttcaa aatctccgcg acaagaatgt tttaaagcca ttcggggatt
      721 ttggggcgga tgatgccgta tgcctcggga tagtcgacgc cggtcaggta aagcccgtcg
      781 ggcatgaagg ttggtggggc tttgaggcgg ctgcgttctt gaatcagtgc ggcgaagcct
      841 tcgacgctga gcctgccgct gccgacataa acgagcgcgc ccatgatgtt gcgtaccata
      901 tggtgcaaaa aggcgttgcc gtgcaaatcg aggcggacga gtcctgcgct ttgggtaagg
      961 tcggcgcggt agatggtttt gacgggggat tttgcctggc actcggcggc gcggaagctg
     1021 gagaagtctt gttcgccgat caaataggcg gcagcccgac gcatcggttc gatgtcgagt
     1081 ttgaggtgtg tccagcctgc cctgtttttg agcagggggg aacggacggg ggcggattcg
     1141 agcaggtagc ggtagtgtcg tccgtatgcg tcaaatcgtg catgaaattc gggggcgacc
     1201 tgtcgagcat gcaaaacggc aattccttcg ggcaggtggg catttacgcc gcgcacccat
     1261 gcctgttggg gacggacggc agttgtgtca aagtggacga cttgggcggt ggcgtgcacg
     1321 ccggtatcgg ttctgccggc aacggtggtg gcgacaaatt ctcctgcgat ttgggcgagt
     1381 gcggtttcca atgccgtctg aacggtcggt acgccgcccg cctgtttctg ccagccgtaa
     1441 aagcggctgc cgtcatagga tagggttatt gcccagcgtt gtttttgtgc ggtatccatc
     1501 ggatttggga ttcggataaa tgttcagact gcattgtatc gcagattttg cagggaaacg
     1561 gcaaacgccc agggcgagcg gcgttgtttg gggagttgtt ggggggggat gcagttgcta
     1621 cgaatcgcta tcctgtgaat ttaccctgtc aggagtgccc gaatcgtcat tcccgcgcag
     1681 gcgggaatct aggacgtaaa atctaaagaa accattttat agtggattaa caaaaaccag
     1741 ta
//
LOCUS       DAWKQB010000211         1732 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.212, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000211 DAWKQB010000000
VERSION     DAWKQB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.212"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            356..1660
                     /locus_tag="WJL14_002004"
     CDS             356..1660
                     /locus_tag="WJL14_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ7657015.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          354 a          432 c          513 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggga tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggagcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga ta
//
LOCUS       DAWKQB010000212         1709 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.213, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000212 DAWKQB010000000
VERSION     DAWKQB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.213"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(351..1037)
                     /locus_tag="WJL14_002005"
     CDS             complement(351..1037)
                     /locus_tag="WJL14_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ7657016.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQISGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWVAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(1114..1587)
                     /gene="queF"
                     /locus_tag="WJL14_002006"
     CDS             complement(1114..1587)
                     /gene="queF"
                     /locus_tag="WJL14_002006"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ7657017.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     regulatory      complement(1591..1635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
BASE COUNT          438 a          372 c          515 g          384 t
ORIGIN      
        1 aagtttgaac cggcgacgaa aacaatgccg tctgaaaatc tgtaaacagg ttttcagacg
       61 gcatttgcag ttgattaaaa taaaattaag ccaaggaagc actgctgtca ttcctgtacc
      121 ggcgggaatc cggacaccac ggcacggaaa cccatccgcc gtcattcccg cgaaagcggg
      181 aatctagaaa tacaacgcgg caggagttta tcggaaatga ctgaaaccca acgcaccgga
      241 ttcccgcttt cgcgggaatg acgaagtggg cgggaatccg gatttatccg ttccgacagt
      301 gtttgcaaat aaaagaaaac ccaaccgtcc cgatttccgg cagggctgtt ttacggattt
      361 tgcagcgagg gcgcggggcg gtcttgcgcc tgtttggttt gcagggtcgt cagttttttc
      421 gtcagcagat tcagaatcac gccgtaggcg ggcaggaaaa acagaccgca gacggtgagt
      481 ttgaacaggt aatcgacaaa agcgatgccc tgccagtttg ccgccataaa tccatcgctg
      541 cttgcgtaga aggcaacggc gaaaaatacc aacgtatcta aggcgttgcc gatgacggtt
      601 gatgcagtcg gggcaaccca ccacgctttc agacggcgta atttgttgaa cacaaaaata
      661 tcaaggattt gtccgagcgc gtaggcggca aaacttgcca gcgcgatgcg tccgacaaag
      721 gtgttgaatt cggacagcgc gcccaagccc gtccaactgc cgttgtggaa caaaacggaa
      781 aagacgtagg aaagcaaaag ggcggggaac atgacccaaa agataatccg ccgtgccaag
      841 tgcgaaccga aaatgcggac ggtcaggtcg gtggcgagga agatgaaggg aaaggaaaac
      901 gcgccccaag tggtgtggat gccggaaatt tggaagggga actgcaccag atagttgctg
      961 gcggcgatga tgaggatatg aaaaagcacc agccagaaga gtgccttctg ttgctgtgcg
     1021 gcggtaaatg cgtacataaa aaaacctttc ggaaaggcgt tcagacggca taccgtatcg
     1081 aaggaatgcc gtctgaaata tgggaaggat ggtttattgt gcgtcgtgct caaacaggcg
     1141 tttacgcgcc aatgcttcaa actcggtgcc tgctttgccg taattggcga aaggatgaat
     1201 ggcgatgccg ccgcgcggtg tgaactcgcc gaatacttcg atgtatttcg gatccatcag
     1261 ggcaatgagg tctttcatga tgatgttgac gcagtcttca tgaaaatcgc cgtggttgcg
     1321 gaagctgaag aggtagagtt tcagggattt gctttccacc attttgatgt gcggaatgta
     1381 gcggatgtag atggtggcga agtcgggctg cccggtcatg gggcagaggc tggtgaactc
     1441 tgggcagacg aatttgacga aatagtcgtt gtcgggatgt ttgttgtcga atgcttcgag
     1501 aatttcaggc gcgtagccgg tcggatatcg ggttttctga ttacccaaaa gcgagatgcc
     1561 ttgcagctct tcggtattgc gggacatagg gtttccttag ttttttagtg tgggaggttt
     1621 tcgaaccacg gggcggcgat tgtaatataa gcggcggtat ctgtgtagtt ttcttcagac
     1681 ggcatggttt ggacggcggc gttttccgt
//
LOCUS       DAWKQB010000213         1673 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.214, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000213 DAWKQB010000000
VERSION     DAWKQB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.214"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(195..1355)
                     /locus_tag="WJL14_002007"
     CDS             complement(195..1355)
                     /locus_tag="WJL14_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ7657018.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNFQLQLTEQP
                     QTANTQQGGKVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRTGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPAQNSKSAYTPAYVDEQSKYHAAVVGKPGSDVYYAG
                     LNYKNGGFAGNYAFKYARHANVGRDAFELFLLGSGSDQAKGTDPLKNHQVHRLTGGYE
                     EGGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKG
                     ENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          428 a          429 c          390 g          426 t
ORIGIN      
        1 tatatgtgca aaccaagccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgcggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcggcattt
      481 tcagacaaat ccaactgggc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttg atcactcccg
      601 ctgccgagca agaacaactc aaaagcatca cgtccgacat tggcgtgtct cgcatattta
      661 aaggcatagt tcccggcaaa accgccattt ttataattca gaccggcata atacacatcc
      721 gatccgggct tgccgacaac agccgcatga tacttactct gctcatccac ataagccggc
      781 gtataggcgg acttgctgtt ttgagccgga acgaattgga cgctgccgct gaaaccggaa
      841 aagtccggag agtcgtagcg tacggaaacc ggcatatcgt cgtggcgttt gaaaataccc
      901 aattgcgaag ccacatcatt attgctgtcc caaggatcaa tggcttggct ggcatcgtca
      961 aactgatttg caacgcggcc ggtgcgcagc gtgccgaatt cgcctgccaa gccgacaaag
     1021 gattccctat tgccccaacg ggtcgcgcca ccgccggcaa cggatacgtc ttgctcaagc
     1081 tgccaaacag ctttcagacc ttcgcccaaa tcctcgctcc ccttaaagcc gataaacgag
     1141 ccgaaatcac tgattttcgt cctgatgcgg cttttggcct tagtaacttt taccttaccg
     1201 ccttgctgag tgttagcggt ttggggctgc tcggtcaatt gcagctggaa gttcctgcct
     1261 tccacgccgg ctttgatttc gccgtacagg ctgacatcgg caacggctgc aagcggcagt
     1321 gcggacaata cgagggcggt aagttttttt cgcatatcgg cttccttttg taaatttgat
     1381 aaaaacctaa aaacatcggg caaacacccg atatgtcttc aattataccc ccccccccgc
     1441 aaaaaaccat ttttcagaac aaatatctga taaatgccgc aacctttatt ttaaaaatga
     1501 ttatatttta atataaaaca atagcttatt ttttcaaaaa ccgttgtgtt tctacaacac
     1561 aattcaagcg cagacctcgt gcgggccgat gcgctgctgc ccggatgcag tctcggcttt
     1621 ttaaaacgcc ataaaaaaac acacgcggca ctttatagtg gattaacaaa aac
//
LOCUS       DAWKQB010000214         1652 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.215, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000214 DAWKQB010000000
VERSION     DAWKQB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.215"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            119..1492
                     /locus_tag="WJL14_002008"
     CDS             119..1492
                     /locus_tag="WJL14_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ7657019.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
BASE COUNT          421 a          536 c          398 g          297 t
ORIGIN      
        1 ctatttgtac tgtctgcggc tcgccgcctt gtcctgattt ttgttaatcc actatatttc
       61 cccgtcctat cggtttcccg tttcagacga cataaggtct gaaagaaaga ctacaattat
      121 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      181 aggttacgaa aacccgacac ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      241 tgatttgcta gccgccgcgc aaaccggcac aggcaaaacc gccgccttta tgctgcccag
      301 tctggaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      361 tatgctcgtc ctcaccccca cgcgcgaact tgccgaccaa atcgaccaaa acgtgcaggg
      421 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      481 acagaccgcc gacctgcgtg ccggctgcga aatcgtcgtc gccaccgtcg gacggctgct
      541 cgaccacgtg aaacagaaaa acatccattt gaacaaagtc gaaatcgtcg ttttggacga
      601 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      661 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      721 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      781 cgccaacgtc gagcagcaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      841 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      901 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      961 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcaacctgcg
     1021 cgtcctcgtc gccaccgata tcgccgcgcg ggggctggat attgccgaac tgcccttcgt
     1081 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1141 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1201 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1261 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccaag
     1321 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1381 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1441 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1501 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaactg gactgacgca
     1561 ttgggcgcaa ccgcccgcac cggataaatt tcttccgcaa acagtttcag acggcatttg
     1621 ccgcctgtac aatatagtgg attaacaaaa at
//
LOCUS       DAWKQB010000215         1602 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.216, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000215 DAWKQB010000000
VERSION     DAWKQB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.216"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..246
                     /locus_tag="WJL14_002009"
     CDS             <1..246
                     /locus_tag="WJL14_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657020.1"
                     /translation="KAKQKKAKQKKAKQKKAKQKKAKQKTAKQEKAKQKKAKQKKAKQ
                     KTAKQKKAKQKKAKQKKAKQKKACLATGFLLIPIFAD"
     gene            complement(243..1232)
                     /gene="porB"
                     /locus_tag="WJL14_002010"
     CDS             complement(243..1232)
                     /gene="porB"
                     /locus_tag="WJL14_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ7657021.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVEHNGGQV
                     VSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQDVDDVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVEDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSVDD
                     AKRDNTYDQVVVGAEYDFSKRTSALVSAGWLQEGKGENKFVATAGGVGLRHKF"
BASE COUNT          443 a          366 c          397 g          396 t
ORIGIN      
        1 aaagccaaac agaaaaaagc caaacagaaa aaagccaaac agaaaaaagc caaacagaaa
       61 aaagccaaac agaaaacagc gaaacaggaa aaagccaaac agaaaaaagc caaacagaaa
      121 aaagccaaac agaaaacagc gaaacagaaa aaagccaaac agaaaaaagc caaacagaaa
      181 aaagccaaac agaaaaaagc ctgtctggcg acaggctttt tgttgatacc aatctttgca
      241 gattagaatt tgtggcgcag accgacaccg ccggcagtcg ctacgaattt gttttcgcct
      301 ttgccttctt gcaaccaacc ggcagaaacc aaggcagaag tgcgtttgga gaagtcgtat
      361 tccgcaccga caaccacttg gtcgtaagta ttgtcgcgtt ttgcatcatc aaccgagcct
      421 ttgaagccgt gggcgtaaga aacgcggggc gttacgttgc cgaagcggta tgccaaggta
      481 gcggcaactt cggtttgaga gttgtgcgaa ttgtcttcaa ccagtttcgc gtcttgttgc
      541 tgtacggcta cggaagcgta tagggcatca ttgtcgtaac cgctgaccaa acggtgaatc
      601 tggtatttct caatcttcac gtcatccaca tcctgatgtc ttttataggc accgccatat
      661 tgcacgaaga agccgccgtt tttgtagttg aagccggcgt ggtaagattc gctgttatgt
      721 ctgcctgcat tgtcgttaag cgcgtattgt acgctgccgc tgaggccggc aaattcggga
      781 gaatcgtagc gtacggaaat gaggcgtgct tcgggttcgg caattttgtt tacacccaaa
      841 tagtcgcttt tgctatccca aggattgatg tcgccggtgt ctttcaggac gctgttcaaa
      901 cggccgacgc gcaatttacc gaagccgcct ttcaaaccga tgaaggattg gcggttgccc
      961 caaccggagt cagtgccggc gatagatgcc ttttgctcaa cctgccaaat ggctttcaga
     1021 ccgttgccga ggtcttcttg gcctttgaag ccgattttcg aacccaaatc aacgatgccg
     1081 gtaccggttt caacgctaac cacctgacct ccattgtgct ctacagagcg ggaagtttct
     1141 acgccggctt tgatggtgcc gtacagggta acgtcagcca ttgctgcaac aggaagggct
     1201 gccaaagtca gggcaatcag ggattttttc attgctgtat tccttttttg gttaagaaat
     1261 ttaagcaggc cgggctttcc aagccgctta gctttgcatt taccgccgac gttttcggcg
     1321 attccatgtc tgaaactata gatgttcctg tctgaaaaaa caaacttctg cctgtttttg
     1381 tgtggaattc gatgtgtatt ttgaagggcg gatagagata ttgtgggtat tttttgtttt
     1441 ataacatatg gttatttaaa ttttttaaga tttgcatttt tacaacactt actcgggagg
     1501 gtattggagg gcattgcaaa ccgggggtta taaagacggc aaaaaacgcg cacgtggttt
     1561 ctttcggaac gggcggatgc aaaacccgcc cctgcggcag ga
//
LOCUS       DAWKQB010000216         1570 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.217, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000216 DAWKQB010000000
VERSION     DAWKQB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.217"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            40..138
                     /locus_tag="WJL14_002011"
     CDS             40..138
                     /locus_tag="WJL14_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ7657022.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(209..1528)
                     /locus_tag="WJL14_002012"
     CDS             complement(209..1528)
                     /locus_tag="WJL14_002012"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ7657023.1"
                     /translation="MKAAAAFATGLDAQAVRIVIDIYGSLALTGYGHGTFDALMLGLE
                     GSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIPDRDLNILGNQVLPKHPNGLRFT
                     AYASDGIVLKEQVYYSVGGGFVVTEEDFDRQAETEKAVPYPYTSCAELLARCRLNRLD
                     ISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIKRGLGADGELPGGLNVRRRAPQL
                     AAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRVVTAPTNGAAGIIPAVLHYFRKF
                     NPHATQERVENFLLTAGAIGILYKTNASISGADVGCQGEVGVACSMAAGAYAEVIGGT
                     PKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNGIAAEKALKLGTLALLEDGTDKK
                     VSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKKAVPVSVRVVEC"
BASE COUNT          286 a          430 c          506 g          348 t
ORIGIN      
        1 cttgccggat gctgcaaaca acgccggttt gcattcctga tggcggtggt tttcttagac
       61 gaacgcccga acacgcagga atggataggc ttggggctgg ttacggcggg cgtgttgacg
      121 ctggcactga aacggtaaag ccgcaagaaa taaatgaaat gccgtctaaa aaactgtttt
      181 cagacggcat tttcgtttct gtccatcctc agcactcgac cacgcgcacg gatacgggga
      241 cggctttttt ccggagcgtg gcggcgagtc cggcaagcga ggtttctttg taggtcgcct
      301 tcatatcccg gccggtttgc agcatggttc ggatgacttc gtcgagcgag acttttttgt
      361 ccgtgccgtc ttccaaaagc gcgagcgtgc cgagtttgag ggctttttcg gcggcgatgc
      421 cgttgcgctc gatgcagggg atttgcacca gtccgccgac ggggtcgcaa gtcagcccca
      481 aatggtgttc catcgccatt tcggcggcgt tttccacttg tttgggcgtg ccgccgatga
      541 cttcggcgta tgcgcccgcc gccatcgaac acgctacgcc gacttcgccc tgacagccga
      601 catccgcacc ggaaatggag gcgttggtct tgtagaggat gccgattgcg cctgcggtga
      661 gcaggaagtt ttcgacgcgt tcctgtgtgg cgtgcggatt gaacttgcgg aaatagtgca
      721 atacggcggg aatgatgcct gccgcgccgt tggtcggtgc ggtaacgacg cgtccgccgg
      781 cggcgttttc ttcgttgacc gccatggcgt acaccatcgg ccagagctgg gtgttgacga
      841 tttcggtttc gcgcaggact ttgagcttgg cggcaagctg cggggcgcgg cggcggacgt
      901 tcaatccgcc gggcagttcg ccgtccgcac ccaagccgcg tttgatgcag ccttccataa
      961 cctcggcaac ggcagcggcg cggcggcgga tttcggcttc gccgcatccg gcaagcgcgg
     1021 cttcgtttgc caacacgact tcggagatgt cgagccggtt cagacggcat cgggcaagca
     1081 gttcggcgca actggtatag ggatagggaa cggctttttc cgtttccgcc tgccggtcaa
     1141 aatcttcttc ggtaacgaca aagccgccgc cgaccgaata ataaacctgt tctttcaaga
     1201 cgatgccgtc tgaagcatag gcggtaaaac gcagaccgtt ggggtgtttg ggcagcactt
     1261 gattgccgag tatgttcagg tcgcggtcgg ggatgaagcg gatttcttgc ccgttgagcc
     1321 ggaggatgtg ctgcgtgcgg atgcgttcga ggcgttcggg aatgccggca agcgggatgt
     1381 cgtgcggcag gctgccttcc aaaccgagca tcagcgcgtc aaatgtaccg tgtccgtatc
     1441 cggtcagtgc gagcgagccg taaatgtcga tgacgatgcg aacagcctgt gcatccaaac
     1501 ctgtcgcaaa ggcggcggct gccttcatcg ggccgaccgt atgcgaactg gaaggcccga
     1561 taccgatttt
//
LOCUS       DAWKQB010000217         1564 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.218, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000217 DAWKQB010000000
VERSION     DAWKQB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.218"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(285..1208)
                     /locus_tag="WJL14_002013"
     CDS             complement(285..1208)
                     /locus_tag="WJL14_002013"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ7657024.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIIGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          431 a          378 c          372 g          383 t
ORIGIN      
        1 catccatacg gaaacctaca ccacgtcatt cctacgaacc tacatcctgt cattcccaca
       61 aaaacagaaa accaaaaaca gaaaccgttc tgtcgtcatt cccgcgcagg cgggaatcta
      121 gaccttagaa caacagtaat attcaaagat tatctgaaag tccgagattc tagattccca
      181 ctttcgtggg aatgacgaat tttaggtttc tgttttggtt ttttgtcctt gtaggaatga
      241 tgaaatttta agttttagga atttaccgga aaaaacagaa agcgttatcc acaagttctg
      301 atgttcagct cgtgaaatgc gtcgggcaaa tcatcgctgt cggcaaattc cacccggtcg
      361 taagccgttt cgtctgccaa aaccgcgcgc aagagtgcgt tgttgatggc gtgtcccgat
      421 ttgtagcctt caaacgcacc gataatcggg tgtccgacga tatacaaatc gccgatggca
      481 tcaaggattt tgtggcgcac aaactcatcg ggatagcgca agccttcagg attcaggaca
      541 tccgtgtcgt caatcacgat ggcgttgttc aaattgccgc ccaaacccag attgtgggcg
      601 cgcatcattt ccacttcgtg cataaagccg aaagtgcgcg cgcgcgcgat ttcgtcgatg
      661 taggatttgc cggcgaaatc gatttcaaaa gtgggcgagc tgcggttgaa aaccggatgg
      721 tcgaattcga tggtcagcgt taccttaaag ccgtcatacg gcgtaaagcg cacccatttg
      781 cccgcttctt tgatttcgac aggcttgagg attttcaaaa aacgcttttg cgccttttga
      841 tcgaccacgc ccgcatcttg caaaaggtaa ataaacggca ggctggagcc gtccataatc
      901 gggatttcgg gcgcgttcag ctcaatcagc gcgttgtcga tgccgtaggc ggacagcgcg
      961 gacataatgt gttcgatcgt gccgacgcgc acgcctttgt cggtaacgat ggtggaggaa
     1021 aggcgggtat cgttgatcaa ataaggggtc agcttgattt gttcgcccat ctcgccgtcc
     1081 aaatcggtac ggcggaagga aatcccgctg ttttcaggcg cggggtgcag ggtcagcgcg
     1141 acgcgttcgc ccgaatgcag cccgactccg gtaacgctga tggatttcgc caaagttctt
     1201 tgcagcataa accgcttcct tatcaatggg gtaagttttg gaataatacg ataaaaccgg
     1261 aaaaacaggc tatgtttttc catagtattt gccaatgtat ccgttttcaa tacgtaagcc
     1321 gcataaaaat gtatagtgga ttaacaaaaa tcaagacaag gcgacgaacc cgccccctcc
     1381 tgaaaaacgc aaaaaatgcc gtccgaaaac ctttcggacg gcattttcgc gtgaaccgtc
     1441 attcccacaa ggacaaaaaa ccaaaacaga aaaccaaaaa cagcaaccta aaattcgtca
     1501 ttcccacgaa agtgggaatc tagttcgttg agtttcagtt atttagaata aattttgaaa
     1561 ctta
//
LOCUS       DAWKQB010000218         1467 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.219, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000218 DAWKQB010000000
VERSION     DAWKQB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.219"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..178
                     /locus_tag="WJL14_002014"
     CDS             <1..178
                     /locus_tag="WJL14_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657025.1"
                     /translation="ALIAAKIDQAEISDSEKSWLKKELGKIKDTALSTLTENAINAIL
                     AATLITLLKKFVGL"
     gene            complement(175..681)
                     /locus_tag="WJL14_002015"
     CDS             complement(175..681)
                     /locus_tag="WJL14_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ7657026.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(684..881)
                     /locus_tag="WJL14_002016"
     CDS             complement(684..881)
                     /locus_tag="WJL14_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657027.1"
                     /translation="MSAPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(878..1144)
                     /locus_tag="WJL14_002017"
     CDS             complement(878..1144)
                     /locus_tag="WJL14_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657028.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(1156..>1467)
                     /locus_tag="WJL14_002018"
     CDS             complement(1156..>1467)
                     /locus_tag="WJL14_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657029.1"
                     /translation="QLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGIEYAVRMDKD
                     FQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYAVRIVFGDVP
                     V"
BASE COUNT          354 a          419 c          319 g          375 t
ORIGIN      
        1 ggctttaatt gcggcaaaaa tagaccaagc ggagatttct gattcagaaa aaagctggct
       61 gaaaaaagaa ttagggaaaa tcaaggacac tgcattaagt acgcttacgg aaaatgcaat
      121 taatgctatc cttgccgcca ccctcattac tcttttaaaa aaatttgtcg gcctctaacc
      181 aaacttcttc tccacttcca ccccaaacgg ctccaatacc tccacaaagg caggcaaatg
      241 tttggggtgt ttttcttgca caaacgccat caagaactca atcaattcca aagccgtcgc
      301 cagttttgac gtttccggca tcacacgcgc attggcggat acggttttcg taaacgcatc
      361 ggccaggctg gccaacagct tggcgcggtc ggacgggggc aaatcttcgg tactcgaatc
      421 ctgcagcatc gtcatcgtgc tgttgtactg caccataaaa ccggccaaca tcgcacggct
      481 caagtcctca ataccgccgc cggccaaagt gtaggcggcg cgcatcttat cccagtcgtc
      541 gcctttttcc ttatccgcac gtttccacgc acgcgcagtg gtctgcggga tttcgcacat
      601 caaggccgcc gtttccaaag tttgctcgcc gctcacatag agccgacgta acttttcacg
      661 gatttcttgc gggtgagcca taattacagt cccattttcg ctttaagcaa ttcccagccg
      721 accgtaatca cgccgccgcc cagtgcgccg aatgtaatgg ccgtgcgttt cgtgtcttgg
      781 cggatttgcg caatttccgc ctgcatttct ttctgatttt tcagcgtttg atcagtcttg
      841 ttttcaatac gcgccaaggc ttccaaaatc ggggcgctca tgatttgtct gctttcctgt
      901 ccagtttttc gtttactttt tccaacttgt tttcgatgcg tcccaacgaa gccgcaatat
      961 tgtcgcggtc tgccttggca tcttgcttgg tgtgataaga gagcttgacc gcgtgcagct
     1021 cctctttcag gtcgtcgata cgcttgtccg cctctttcag acggcctgaa atgccgttga
     1081 cccaaaacca aaatgccgcc gtcgcaatcg gccacagggt tctgaaacca aattcaaagt
     1141 ccatttaaaa cccctttaaa ccggcacatc gccgaatacg atacggacgg cgtaaccgtc
     1201 agggcgattg ctggcaatct ccagtccatc accatcatta caaacacagt aatacgcccc
     1261 caaagtttgc caaaccgcac gtttaaaggt gtcatagttc gtaattggat attcaaggtg
     1321 aaacgtcgtc tgaaaatcct tatccatgcg taccgcgtac tcaatgcctg ccttatccaa
     1381 cagatcggaa acatgaatga caaacggctc ttgctcgcgt gcgcggctta agcccaattc
     1441 caaatcggca tggcgcacag ccaactg
//
LOCUS       DAWKQB010000219         1445 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.220, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000219 DAWKQB010000000
VERSION     DAWKQB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.220"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            27..1364
                     /locus_tag="WJL14_002019"
     CDS             27..1364
                     /locus_tag="WJL14_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ7657030.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRVLRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPVGALSVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          263 a          340 c          390 g          452 t
ORIGIN      
        1 tactttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtcct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaattccctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttactatct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacgggc ggtaaaaatg
      241 ccgtcgattc cttcagggtt ctgcgtccgg gcacgcaatg gctttgggtc gggcgtatgg
      301 gcgttgccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattga
      361 attatgtcgt ccacagtttt acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggctctg tttatgctga
      481 ttacggtttg ggtggtcaaa ggtgggattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggccattttc attgtcggca
     1081 tcccatccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac cttgccccga
     1321 ttgccattat tattgttttt atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaaacc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaa
//
LOCUS       DAWKQB010000220         1423 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.221, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000220 DAWKQB010000000
VERSION     DAWKQB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.221"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(52..582)
                     /locus_tag="WJL14_002020"
     CDS             complement(52..582)
                     /locus_tag="WJL14_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ7657031.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(623..>1423)
                     /locus_tag="WJL14_002021"
     CDS             complement(623..>1423)
                     /locus_tag="WJL14_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7657032.1"
                     /translation="RTPNIKYIYQDNQGTAEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSACNKIIKRELIIKEKLFFEKEVYAEDIEWSMRLFNAATTFSYLDGCYYYYRQGRK
                     DSKTGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
BASE COUNT          382 a          346 c          267 g          428 t
ORIGIN      
        1 tgcgtcccgt ccgcgtgaat ttggggctga cgtaggaacg ccgatgtgat ttcacatccc
       61 gtactgtttc gacagcttca cataatgcgc ggcggaatat ttcaaaaagg ctttttcctc
      121 atcggtcagc acgcgcactt gtctgaccgg cgaaccgaca taaaggtagc cgcccgccaa
      181 acgtttgcgc ggcggaacga ggctgcccgc gccgatcatc acttcgtcct caatcacggc
      241 atcgtccaga accgtcgtcc ccatgccgac caggacgcgg ttgccgatac ggcagccgtg
      301 cagcatcact ttgtgcccca cggtaacgtc ttcgccgata accagcggcg acccttcggg
      361 tttggcggcg gttttgtggg aaacgtgcag gacgctgccg tcctgtatat tgctgcgcgc
      421 gccgacggtg atgctgttca catcgccgcg caacacggcg cacggccaca cagaaacgtc
      481 ttcggcaagc gacacttcgc cgatgacgac gcacgcttcg tctatcatac agctttcgtg
      541 gatttcgggc gtgcggtttt ggaaagttcg gattgcgttc atttttcctc cttcggtaag
      601 atgtatagtg ttaaaggatt tattaaatat tccccctgat tgcttttaaa atcctgcctg
      661 taatatcgac cccgaataat gtgattatcg ggaatatcag cttatatatc aatttattgg
      721 actttaacag cataaacctt aaatgatacg cccttctttt tatatcagca tcacacgcta
      781 tatttttact cgtcatgata aaaagcaaaa cgagatattc gtaggaaaga aaagaataaa
      841 gataacttga tatatcccta ttaaattcca tttccgcatt tttctccaaa atatataata
      901 atgactttat acttttttcc gaaacagttc ctgttttaga atcttttctt ccctgccgat
      961 aatagtaata acaaccgtcc aaataagaaa aagttgttgc cgcattaaat aatctcattg
     1021 accattcgat atcttcggca taaacctctt tttcaaaaaa tagtttttct ttgataatca
     1081 attccctttt tataatctta ttacacgccg aacccggaaa ttttctaaat cggcataatc
     1141 ctttcaaaac ttcgactttg gattgattga gtattttttc aggctgataa tcttcgccaa
     1201 aatgtgaaac acttccctta tcatatttaa ccgcatttaa aaaaaccaca tccggcatat
     1261 caatatcatc tttactaaga aaatccagca agacctgaca attaataaaa tcatccgaat
     1321 caataaagac tatatatttt ccgtttgaat tttttattcc ggtattccta gcttctgccg
     1381 tcccttggtt atcctgatag atatatttga tatttggtgt ccg
//
LOCUS       DAWKQB010000221         1399 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.222, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000221 DAWKQB010000000
VERSION     DAWKQB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.222"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(157..492)
                     /locus_tag="WJL14_002022"
     CDS             complement(157..492)
                     /locus_tag="WJL14_002022"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657033.1"
                     /translation="MPIPCHSGNTTIPAARASFMSGRVRAQPDGDAAQTGRRQCRRYF
                     VKCLIRRDFIGYRLAARTSRRVKLSSLTDTGFPRTGGFGNYANFPIPILNRQFDRFVE
                     ICAAFHVKP"
BASE COUNT          402 a          352 c          373 g          272 t
ORIGIN      
        1 tttgatttta attgaatata caaaccacag tggggcgtgg tcgttgtaac gccaaaaacg
       61 caacatctac aatacaccga aagcaatggg atagcaatta aaaccgtaca ccgccgcacc
      121 agtttcgaca gctgccgcgc aacagcaggc aaaaaatcac ggtttgacgt ggaaagcggc
      181 gcaaatttcg acaaaacggt cgaactgccg attgaggatg gggatgggaa aattggcgta
      241 gttgccgaac cctccggttc tgggaaaacc ggtatcggta aggctcgaga gctttacgcg
      301 ccgacttgtg cgggcggcaa gccgatagcc gatgaaatcg cgcctgataa ggcatttcac
      361 gaagtaacgg cggcattgtc ggcggccggt ttgggcagcg tccccgtccg gctgcgcccg
      421 tacacgcccc gacatgaaag aagcgcgtgc cgccgggatg gtcgtattgc ccgaatggca
      481 gggtatcggc atcggcacac ggtttttaaa cacccccagc gaaatgcggt accgaggcgg
      541 aaaccgctat aataaaccca tgcggacact gttccacacc cccaccccgg gcttgtccgt
      601 tccttgcgga aagaccgcaa atggacgcag atttccgccg ccctatacgg acaaaacaag
      661 acccgatccg ccgaagccgt cacgcggagc ttcaaaaaaa accccgctgt aaacggcaag
      721 agcaaagggg caggcgcagg atacggcgga cacctccgcg ccatacaggg attccgatac
      781 cgggcaggga cgctccggcc gacagttacg gcgcaaatta agcgggaaca tctcaaaaaa
      841 caagcccgat tggaagaatc cgatcgggct tgttttgcaa ctgttttgtc atgccgtgaa
      901 atatccgcat gacaaaataa tgcaaaattg ggacaaaacg ggcgcgaggt tacacaagcc
      961 cgtttgagag gagtatttat tgagaacgaa ccgtgggcgc gttgtttcaa gagatataac
     1021 aatagcatac cttcttctac gcagacccgc cgtactggca aaccgcaggg tacgaccacg
     1081 aattcgattg gctgcagtat gagctgctga ccaaagcgat ggcggagagc aagggaaaat
     1141 tcatgctatc tatcaatgac catcctgata taagggagtt tcaaagactt ccgcatcaca
     1201 cagcctgagc ttgcctatac ggttggcaga gacaaaaccg gtaaaacaag cggcgagctg
     1261 attatatgca attggtaaaa caaaagggca gcttacaata cgtttttcgg caaagcaagg
     1321 gcatctgaaa agttttgcaa aattttcagg tgtccttttt tgttttcaaa gtgaatgaat
     1381 gaaatactac ggtcagaag
//
LOCUS       DAWKQB010000222         1381 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.223, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000222 DAWKQB010000000
VERSION     DAWKQB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.223"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..329
                     /locus_tag="WJL14_002023"
     CDS             <1..329
                     /locus_tag="WJL14_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ7657034.1"
                     /translation="IATTVPAQQAFLDAAELMQWSIETEGLGLNVISHKILGKDHAQV
                     EFEAYFRDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGK
                     YLKPVA"
     gene            complement(546..1340)
                     /locus_tag="WJL14_002024"
     CDS             complement(546..1340)
                     /locus_tag="WJL14_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ7657035.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
BASE COUNT          370 a          422 c          305 g          284 t
ORIGIN      
        1 tcatcgctac taccgttccg gcgcaacagg cattcctcga tgccgccgaa ctgatgcaat
       61 ggagtataga aaccgaaggg ctgggcttga acgtcatctc gcacaagata ctcggcaaag
      121 accacgccca agtcgaattt gaagcctact tccgagacgg acaacaccga tccgcgcatc
      181 acgaactgtc cggcttcgtc aacatcggcg gacaatggta ttttatcgat cccaccgttc
      241 cgcatcctgc gatgaaacaa ccctgcattt gcggatcagg caaaaaattc aaagcctgct
      301 gcggcaaata tctgaaacct gtcgcataaa aatgccgtct gaacgttcag acggcatttt
      361 caacgtgcaa aaaaaccatt cataccaagg gtaagtatga atggtcaata cattgcggga
      421 aaacgtctta cttgctgcac tgccgaaaag ggagaaacgg cagcggtaat cagcggaaag
      481 gattgtaccc gaattaatgt taagaaacgt taatcgcgaa aatatattaa caaacctgtt
      541 gaaacctatt ggttttcccg tatccacccg acccagcgtt caaacagctt cggttcgagc
      601 gcggcaacga ccgagcgttt gaacacgtgt tcaccactcc aaaacccgtc gccttccaaa
      661 gtcgtcagcc tgccgcccgc ctcctcaaaa atcaacgcgc cggcggcata atcccacagc
      721 ttctgcccgc cgtggacata aacatcataa cgcccgcacg ccagataaca ccaatccaac
      781 gtactgctgc ccatactgcg tatcgtcccg aaaggcgcga gcgtactcat gcggctggac
      841 agtttgcccg aacgcagata tttgatttcc acacccgcaa ttgcctcatt caacttctta
      901 tccacgagac gcaaaggcag gcgggttcca ttaagaaacg caccctgccc ccgttcggca
      961 taaaaacatt cgccgctgac cggattgtaa atcacaccca attcggcgcg cccgttgcgg
     1021 acaaacgcca ccgacacggc aaaatgcggc agcccgttga caaaattgtt cgtcccgtct
     1081 atcggatcga caatccacag ccccttttcc cccgaatatt gttcccacaa agccgactgt
     1141 tcctgccgcg acatttcctc gcccaacatc gggcaatcga tcagaagcgg caacgcggcg
     1201 gcaaaagccg tctgcgcggc aatgtccgcc tcgctcaaca tcgaaccgtc ttccttgcgg
     1261 tgagacggcg tattcaaaaa acgcggcata atttcggttt gcgcgatatg gcgcacgact
     1321 ttctgcaaac ggtgtaacac ttcctactgt cctcatattt tgaacttgcg gcacgcgaac
     1381 g
//
LOCUS       DAWKQB010000223         1287 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.224, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000223 DAWKQB010000000
VERSION     DAWKQB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.224"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            166..1278
                     /gene="nadA"
                     /locus_tag="WJL14_002025"
     CDS             166..1278
                     /gene="nadA"
                     /locus_tag="WJL14_002025"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ7657036.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKETNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT          305 a          342 c          384 g          256 t
ORIGIN      
        1 accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa cctgttttca
       61 gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca aaatataaac
      121 ggcaaatctt tatgccgtct gaaaaaccag ctacaaggaa acatcatgca aaccgccgcc
      181 cgccgctcgt tcgactacga tatgcccctc atccaaacgc cgacttccgc ctgccaaatc
      241 cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg agacggcagg ccgtctgaaa
      301 gacgaaatca aggccttgct gaaggagaca aacgcggtct tggtggcgca ttattatgtt
      361 gatccgctga ttcaggattt ggctttggaa acgggcggat gcgtgggcga ttcgctggaa
      421 atggcgcgct tcggcgcgga acacgaagcc ggtacgctgg tggtggcggg cgtgcgcttc
      481 atgggagaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc tgatttggag
      541 gcggaatgtt ctttggattt gggttgtccg gaagaagcgt tttcagcgtt ttgcgaccaa
      601 caccccgacc gcacggtggt ggtgtacgcc aacacttccg ccgccgtgaa agcgcgtgcc
      661 gactgggtgg taacgtcttc agtggcgttg gaaatcgtgt cgtatctgaa atcgcgcggc
      721 gagaagctga tttggggacc tgaccgccac ctcggcgact acatccgccg cgaaacgggc
      781 gcggatatgc tgttgtggca gggttcgtgc atcgttcaca acgaattcaa agggcaggaa
      841 ctggcggcgt tgaaggcgga acaccccgac gcggtggtgc tggtacatcc agaatcgccg
      901 caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct gaaagctgcc
      961 gtatcacgcc ccgaaaaaaa attcatcgtg gcgaccgatt tgggcatcct gcacgaaatg
     1021 caaaagcagg cacccgacaa acaatttatc gccgcaccga cggcgggcaa cggcggaagc
     1081 tgcaaaagct gcgcgttctg cccgtggatg gcgatgaatt cgctgggcgg catcaaatac
     1141 gccctgacaa gcggacacaa cgaaatcctg ttggacagaa agctgggcga agccgccaaa
     1201 ctgcctttgc agcgtatgct tgacttcgcg gcaggactca agcgcgggga tgtgttcaac
     1261 ggcatggggc ccgcctgatt tgccgtc
//
LOCUS       DAWKQB010000224         1253 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.225, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000224 DAWKQB010000000
VERSION     DAWKQB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.225"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            236..1189
                     /locus_tag="WJL14_002026"
     CDS             236..1189
                     /locus_tag="WJL14_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ7657037.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPTSFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAVQYTVMPLLAIGIAKLFDLSLGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFLGNAESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          197 a          268 c          436 g          352 t
ORIGIN      
        1 aaccggatga ttcgggctaa ataattcctg attctttgga tatagccaac ggtatgcctg
       61 ccgatgccgt cagaaagggc ttgatgggcg tttgcggttc gggttaatat ttgtgctgta
      121 tctgttaatt ccggttagta aaatgggggc ggggacgatg agtggccgtc gaccggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccgacttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggtgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgctggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggcg tttgcctgca ttgtcggcgg cgtggcttcg gttcacgggc accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacgggattg agcgcgaaat tttttctggg
     1081 caacgccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gcc
//
LOCUS       DAWKQB010000225         1247 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.226, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000225 DAWKQB010000000
VERSION     DAWKQB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.226"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            114..338
                     /locus_tag="WJL14_002027"
     CDS             114..338
                     /locus_tag="WJL14_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657038.1"
                     /translation="MMKNKYILTKAAIGITAISIFYLLNQESSGKTEEPSYFWMFMFL
                     NLLWFEENKTVMAAVTAIIAAHFIFVALSD"
     gene            480..848
                     /locus_tag="WJL14_002028"
     CDS             480..848
                     /locus_tag="WJL14_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="wall-associated protein precursor"
                     /protein_id="HEZ7657039.1"
                     /translation="MANGLDNQAFEDQVFHTRADAPMQLAELSQKEMKETEGAALWFA
                     PVLAGGGRFALTGFTRHGLNQAISRGGVGVSNKAIMNTMRNPTKITSQSGGRTRFTGA
                     DGGVVLNSQGKVITTWGKPR"
     gene            894..1142
                     /locus_tag="WJL14_002029"
     CDS             894..1142
                     /locus_tag="WJL14_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657040.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          402 a          208 c          254 g          383 t
ORIGIN      
        1 aggcaacgct gtactggttt ttgttaatcc actatatatt tatctgatat atcaacatct
       61 ctctatctta ttccgaattt tattgttgaa acttgcatga aaaaccatac aagatgatga
      121 aaaacaaata tattttaaca aaagcagcaa tcggcataac cgccatatct atattttatc
      181 ttctaaatca agaaagcagc ggcaaaactg aagaaccatc atatttctgg atgtttatgt
      241 ttctcaactt gctttggttt gaagaaaata aaacagttat ggctgccgtt acggcaataa
      301 ttgccgccca ctttatattt gtcgcactat ccgattaatt aagcaatcaa aaaggctgct
      361 tggaaatttc aggcagcctt tcaggaggat tttgatggaa cattatctcc tccctaaaaa
      421 tttgttatga aaaaacaaat cactgaagcc acgatgatgc tgtctataat cgccccgcaa
      481 tggcaaacgg cttggacaat caggcatttg aagaccaagt gttccacacg cgggcagatg
      541 cgccgatgca gttggcggag ctttctcaaa aggagatgaa ggaaactgag ggggctgcat
      601 tatggtttgc tcccgtgttg gcaggtggtg gcagatttgc actgacagga tttactcggc
      661 atggtttaaa tcaggctatt tctcgtggtg gtgtgggagt ttcaaataaa gcgattatga
      721 acactatgcg taaccctaca aaaattacaa gccaatcagg aggaagaacc cgttttacag
      781 gagctgatgg tggtgttgtt ttgaatagtc aaggtaaagt aattactaca tggggcaaac
      841 ctcgttaatc aaaaaaaggg ggctttgccc cttttataac ttaagggtaa tttatgaata
      901 ttaaaaatga aatagttgct ttaatttcta aaaaaattga tggggggcaa tatagttctt
      961 tagaaaattt tgctttatcc gtactaaggg aaaaaattat aaaaaatact gtttccagtg
     1021 atgaaaaatt attgattatt aatgattttt tatcaggttt tcttgagttt gatcctgaga
     1081 ccgggaaacc tgtcggggag acgcttaaaa ttgagcaaat gattgatttc tttttagtct
     1141 aaatcataca taccagtgaa caagctaggc gtatatccgg cttttaaata cgattgtcag
     1201 gtttatactt gggataaaat caaaaataaa gttgtttaaa ctctagt
//
LOCUS       DAWKQB010000226         1226 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.227, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000226 DAWKQB010000000
VERSION     DAWKQB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.227"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(39..497)
                     /locus_tag="WJL14_002030"
     CDS             complement(39..497)
                     /locus_tag="WJL14_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657041.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
BASE COUNT          436 a          224 c          130 g          436 t
ORIGIN      
        1 atttgcaatc gattccgttg tattttcgca agacacgttt agtctagagt ctgtatatta
       61 catttatttt tagggtctgc tagccaattt cttgttccct tcattatttt atcttctgaa
      121 agaaaattat ttttttccat gctattaata ttaatgatat gatttttatt taaaataatt
      181 tttccatata aaatttccgg tttgtaatag caagagtctg aagcaaatcc ttgcccgtca
      241 atataagaaa aataaatgtt atcatcctcc ttataatatt ctaaaaccaa tccggatatt
      301 attttatcaa aattcccttc atttccatct ttcatgacta aaatataatt agcaggccaa
      361 cctttatcgt aaacatattc aaactgatag ttcccgaaac tctcgatatc cgaactaaaa
      421 aagaagaaaa gcaaattaat agccaataca aaaacaaata agaacaaaaa tagcaaaatt
      481 ttcaacttag ttaacaatat ttacctctcc tttaaattca atcctgaaag gtacccctta
      541 ccctgggcta ccaattatag tttccatatt tctaaatatt gtttttacat tacttttttc
      601 tccccccaaa ggaatgcatt ttaaaatcat gcttttcagg tgctaatcga tacttaccat
      661 taacatcttt aatcacagat atatttccat gtatagccca acgtgaaaaa tctgagtatt
      721 atatacagtt ataccttact gaaacaaagc tagctgtaca tatctgtacc atatttttac
      781 tacaaacaaa aaatatttat aattattaag gataagataa aaacccccaa cgccagatta
      841 attcttaata atttcaaata aaaaatattt gagatttttt cttttccaaa taatatattg
      901 aaatatttaa agttaaggta gcttgattta agtagaaaat caaaaaaacc attaattaat
      961 ttttcttctt taaatatttt atatgatatt ataaaattaa ataacattgc gaaaacatat
     1021 gaaatcatca aaataccaaa tatatagtgg gaatgcatta ttatttagct ccttcttttg
     1081 gctctgacaa attgacagtc cattgatatc caatgcttgc atttgcaatt gctgacgcac
     1141 ctgcccctgt tttccctgtt agtatcaatg gttcttttct tgcaagatgt gcacctgcac
     1201 aaccacctaa tactaaacca ccacct
//
LOCUS       DAWKQB010000227         1193 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.228, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000227 DAWKQB010000000
VERSION     DAWKQB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.228"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            255..>1193
                     /locus_tag="WJL14_002031"
     CDS             255..>1193
                     /locus_tag="WJL14_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7657042.1"
                     /translation="MNKTLKRQVFRHTALYAAILMFSHTGGGGAMAQTRQYAIIMNAQ
                     NQPEVRQNGQYSTLREKDRKRKYIHHNYTQGGSSVSFDNSDELVSQQSGTAVFGTATY
                     LPPYGKVSGFDDKRLKERGNAVNWIHTTHPGLIGYSYAGVVCRDSTGCPKLVYKTRFS
                     FDNPDLAKTGGVLNRHTEPSRENSPIYKLKDHPWLGVSFNLSAEGTAKDGKTINKLVS
                     SFNENNNNQTIVSTTEGSPISLGDGQREHTAMAYYLNAKLHLLDKKQIQNITDKTVQL
                     GVLKPSIDVRKGAGLLSYWARWDIKDDGQIPVKLDLQ"
BASE COUNT          333 a          312 c          301 g          247 t
ORIGIN      
        1 cagcaattcg gcgggcgcgg aatcgggcgg tttgcccaat cccgccgttt gcggcgcgcc
       61 tgccgccgac ggtatcccgc gaagcaagat ttaagggata aaatatgttc caacacgcag
      121 ggcggcacat caagcaccgc cctatgtgcc gccctgattc ggaagggctt gcacccctcc
      181 cggacaaagc ctgatcctgc cgccccgaag gacggatgtc cgagcggcgg ggtttcaacc
      241 gaaaaggaaa tacgatgaat aaaactttaa aaaggcaggt tttccgccat accgcgcttt
      301 atgccgccat cttgatgttt tcccataccg gcgggggggg ggcgatggcg caaacccgtc
      361 aatacgctat tatcatgaac gcgcaaaacc agcccgaggt acggcagaat gggcaatatt
      421 caaccttaag ggaaaaagac aggaagcgca aatatattca tcataattac acacaaggag
      481 gaagctctgt ctcattcgac aatagcgatg agcttgtttc ccaacaaagc ggtactgccg
      541 tttttggcac agccacctac ctgccgccct acggcaaggt ttccggtttt gatgacaaaa
      601 ggctgaaaga gcgcggcaat gccgttaatt ggattcatac gacccaccca gggttgatag
      661 gctacagcta cgccggtgtc gtatgcagag acagcacagg ctgtcccaaa cttgtctata
      721 aaacccgatt ttccttcgat aatcccgact tggcaaaaac aggaggcgtg ctgaataggc
      781 acacagagcc aagccgcgaa aattcgccca tttacaaatt gaaagatcat ccatggttgg
      841 gcgtatcttt caatttgagc gccgagggta ccgccaaaga tggtaagaca atcaacaaat
      901 tggtatcttc ttttaatgaa aacaataata atcaaaccat cgtctctacg acagaaggct
      961 cccctatttc ccttggcgac gggcagcgcg aacataccgc catggcctat tatctgaacg
     1021 ccaaactgca cctgctggat aaaaaacaga ttcaaaatat caccgacaaa acagtgcagt
     1081 tgggtgtctt gaagccgagc atcgatgtgc ggaaaggtgc cggcttgcta agttattggg
     1141 ctaggtggga cattaaagat gacgggcaga ttccagtcaa gctcgacctg cag
//
LOCUS       DAWKQB010000228         1169 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.229, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000228 DAWKQB010000000
VERSION     DAWKQB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.229"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(231..1085)
                     /locus_tag="WJL14_002032"
     CDS             complement(231..1085)
                     /locus_tag="WJL14_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ7657043.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCSW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSVLGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          328 a          347 c          308 g          186 t
ORIGIN      
        1 tcccgtcatt cccgcgcagg cgggaatccg gacatccaac gctgcagcaa tttgtcggaa
       61 ataaccgaaa ctcaaaaaac cggatgcccg cctgcgcggg aatgacgatg caaagatggt
      121 cgttatttcg gataaatatc cgtcttttcg ttccggatac ccgctttcgc aggaatggag
      181 atggaaagat ttcagcctat tcggcaatca tcaaataccg cttcccaact ttatttttga
      241 tgcgacagcc ggccggcaac caaggtggcg gcgatgacga caaacacgcc caagacggac
      301 acgggcgaca ggtgttcgcc caaaatcaaa accgccagca gcacgccgac gacgggttcg
      361 agcgaaatca acagtcccga aacgttggca ggaacacggc tcatcccctt gttccacagc
      421 caataggcgt accagctgca ccccacgccc aaatacagca gcgacaatac cattccgacg
      481 ctccagtcca cggtataact ttgcgccaaa gcaagcgaaa acggcaggca catcaacgat
      541 gcggcggcaa tggaaacaga tgtgaatgcc ggtgcgccga tgcgtgcaat cagcctttgc
      601 gtcggacgca tagcggcaca aaagcccgcg cccgccaaca acaccagcag gcagccgaac
      661 cagccgactt cgccgccctc ttccgcaccg cccgccatca gcagcgcgac accggcaaat
      721 gccgccgcgc cgcatatcca gtggtaggca cgcgctttgt cgttgaagaa aaagtgtccg
      781 acaaacacca tcagcagtgg ctcgagtccg acaatgaccg atgcgctggc ggcggaagtg
      841 tatttcaacc cgacaaactg aagcagcagg gtcagcacat agttgacgaa cgacacaatc
      901 agcaacggct tccattcctc acgcggaatc ttgccgacat gacggcggca ggcgggcagt
      961 gcaggcagcg cagcaatcag caggcgcacg ccgaccatca atgcgggatc gatgccgcca
     1021 tagacatatt tggcggcaat aaacgagctg ctccagataa tcagggcaag gatttggtaa
     1081 aacatagtga gtgataaagt cagatggtca tacaaaagta tagcggatga acaaaaatca
     1141 ggacaaggcg gcgagccgca gacagtacg
//
LOCUS       DAWKQB010000229         1169 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.230, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000229 DAWKQB010000000
VERSION     DAWKQB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.230"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(267..908)
                     /locus_tag="WJL14_002033"
     CDS             complement(267..908)
                     /locus_tag="WJL14_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ7657044.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIAFPRKTMDYLIGMSEKSGVPLYFDRAL"
BASE COUNT          338 a          287 c          223 g          321 t
ORIGIN      
        1 tttatttaaa aaataatact attctgaaca ttatttagat acagaaatta acaaattaga
       61 actaaacaag cttttaaata ctttaatttt attggaaagc tataaaagga actataactt
      121 tacacactag tcacttcttt ttaagaggca aaagggattg ggaaggtcgt cttggagata
      181 agcactggta tttcggccaa tggtaaatag agtttacctc aaatagggta gaacctcctt
      241 catctgtcag ttaataacag ccacttttac aatgccctgt cgaaataaag tggcacgccc
      301 gatttttcac tcataccgat taaataatcc atcgtcttcc gagggaacgc gatgccgcgc
      361 accacggtca gattcctcaa aacggggaaa accaaaatat cctccatacc gattccgccg
      421 ttgatgccgt ctgaagcacc gtccatcaaa ttttccaact cttgcaaatc tgcgtttatg
      481 cgttcgagat attgggcggt tttattcaaa ttggcggaaa agctgccgat gcttttctct
      541 tttttgtctg taaaatattt taccgcttcc ggcgttgcaa attcgggcag cccgattttg
      601 atcacgcgcg gctgcaccag tttgtcgttg tatccgccca ccttgtccag ccacgcccgt
      661 atctcggggc ggacttcgtc tttcagacgg tcttcgcggt cgaaatgccg cacaatgtcc
      721 aaactctcgc ccataaacga accgtcttct ttttgcagga cgggcacttg tttcgcaccg
      781 atcataccga tcggcgttgc ctcgtcgtcg tttgccagca cggcttcttc aacgtccgcg
      841 ccaaacagcc cggcagccat ccgcgcacgc acgcaaaacg ggcaatggtc gtaaatatac
      901 agtttcatca aaatattcct cgtcaacctg tcggtaccgg ctaccttaac acccggcgcc
      961 gcccgaaaca agtttatctt cccacctatg caccgtaaat aaataagctg agacctttgc
     1021 aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg ccaaaaaatt ttcaattgcc
     1081 taaaaccttc ctaatattga gcaaaaagta ggaaaaatca gaaaagtttt gcattttgaa
     1141 aatgagattg agcataaaat tttagtaac
//
LOCUS       DAWKQB010000230         1134 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.231, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000230 DAWKQB010000000
VERSION     DAWKQB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.231"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(123..551)
                     /locus_tag="WJL14_002034"
     CDS             complement(123..551)
                     /locus_tag="WJL14_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ7657045.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(532..966)
                     /locus_tag="WJL14_002035"
     CDS             complement(532..966)
                     /locus_tag="WJL14_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ7657046.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKASGDDGVRKS"
BASE COUNT          299 a          336 c          203 g          296 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcatccc
       61 ctgcgaacca aaaaaaagac cgtctgaata cagacggcca aagcctggtc acacatcact
      121 gcctaaaata aagctgcctg ctgtgccgca ggtcggctca tttcattgat aatcgtatat
      181 cccgttcgtg aagagatgcc gtatttaggg catagcttcg tcatcgccat aagcccgctc
      241 tccttatcaa tatcgcgcaa tttgacaaac tcctgataaa acctatggtt tctcaactgt
      301 attaacgcct tgccgcaccg tgggacatac aattcctcgc caccatatac ctgcaacagc
      361 tcatgtgttt tcacttcgcc gatggcttcg accaaaattg ccaaacgctc ggtgtccacc
      421 ttgcccttac caaatttaaa ccgcgccccg ccaatcgcct tgaccagctg ttccgtcgct
      481 gccagtccga tgacatccac aatgtccaac acggtatccg gcaataaatg ttcaactttt
      541 tcgaacccca tcatccccac tcgctttttc cgttttcctg ttttccgcaa tctgcaacgc
      601 agcaaccagt ttatgtagct gcgtatcgtc taaatattcg accttatcct taccaaacat
      661 ccgccgcgcc attgcgtgtg catagttcca atgtttgccg ccgacggtca gcagggcttc
      721 gactttgtcc agcattgccg ctgatgatgt ccgacgcaga tgcggtttgc cgtgtgggtt
      781 accttttgct ttaggcttaa atccgtgcga ccgcatatca gcgacaacag actcaagttc
      841 agaaacatcc atatccgcac acgaccgctt atccgtcaca cgctccaaca ccgcgcgata
      901 ggtaccgtca tccaagccca gctccttttg agcaatctta attttcgcaa tcaacgcccg
      961 gcgcattatc tctccaatac aacatatagt ataaattagc gcattttata caataaaata
     1021 caatatatag tattaagccg atgttttttt gcgaaacgga cagacataaa aaagcaactg
     1081 tatttttcac cccgtcgggc aaaaatacca aaactcaaat caagccgtcc ggat
//
LOCUS       DAWKQB010000231         1112 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.232, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000231 DAWKQB010000000
VERSION     DAWKQB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.232"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            153..1052
                     /locus_tag="WJL14_002036"
     CDS             153..1052
                     /locus_tag="WJL14_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ7657047.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQS"
BASE COUNT          187 a          256 c          355 g          314 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagcctgcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtctt aatattgatt ccatcaaggc agtattggca
     1081 gttttccgtg caaaacctgc gttaccggac tg
//
LOCUS       DAWKQB010000232         1080 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.233, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000232 DAWKQB010000000
VERSION     DAWKQB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.233"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(<1..669)
                     /locus_tag="WJL14_002037"
     CDS             complement(<1..669)
                     /locus_tag="WJL14_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ7657048.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGID"
     gene            954..>1080
                     /locus_tag="WJL14_002038"
     CDS             954..>1080
                     /locus_tag="WJL14_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ7657049.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQK"
BASE COUNT          294 a          259 c          230 g          297 t
ORIGIN      
        1 gtctatgccc gatatacaat tttgatacac aaacttggaa atatcggtat cgtcgccgga
       61 gcgatagaat gcggacagtt tttcattgaa ttgcggcatc tgcatttcgg agatttccaa
      121 gatgccgcag ccccccgcca tcatcagacc gttggcaaat aaggttgccg ttcgtttgtt
      181 gccgtcccaa aaaacctgct gccgcataca ataaagcata aaacggacgg ctgcctccgt
      241 cgtcgaaccg ctttgcagtc cgatattttg taacacgcgg gccacttcaa tttccttcac
      301 tggattcggg gcatgacggg aaccgtccaa tagcgttacg ccgaccgaac cggtacggaa
      361 atcaccgggt gccaaagaat cgtccttggc aacaatgttg ttgatttttt taaggagtga
      421 aatatcgacc ggttcgccgt ttgaaatatg cgaaatcaca tattgatagg cacgtttcag
      481 gttcaagatg gtttggatgt cttccagtga cgcggaggct acatttttgc catggataat
      541 ttgctcagtc tgcaataagg tcgtctgaca attctcaaaa cggctgaggt tgtgaatttg
      601 ggcaaccaat actttctttg ccaaaaaaat attttctttc aaactcaact tgtatttgtc
      661 gggaaacatg ataattcctt aaataattaa agcagtacgt taaatcatca tcttatcatc
      721 actttcgagg tttatgttgc gtgcgtccgt tttctaatac ggtttcccat cccgcgaaac
      781 gaggcggagg cctgtgcaaa aaacctgttc acgaaatttt ccgcattaac gatacgctga
      841 aaatgcgcta aaaatgactg tgtttgaata tcggttgatt tcatccgcct gagacctttg
      901 caaaattccc caaaatcccc taaattccca ccaagacatt taggagattt ctcatgagca
      961 ccttcttccg gcaaaccgcc caagccatga ttgccaaaca catcgaccgt ttcccactat
     1021 tgaagttgga ccgggtgatt gattggcagc cgatcgagca gtacctgaac cgtcaaaaaa
//
LOCUS       DAWKQB010000233         1035 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.234, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000233 DAWKQB010000000
VERSION     DAWKQB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.234"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            32..>1035
                     /gene="tuf"
                     /locus_tag="WJL14_002039"
     CDS             32..>1035
                     /gene="tuf"
                     /locus_tag="WJL14_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ7657050.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     "
BASE COUNT          281 a          287 c          256 g          211 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgtccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggtcctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatc gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaattggctg ctgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaacct ttcttgttgc ctatcgaaga cgtattctct atttccggtc gtggtacagt
      661 agtaaccggt cgtgtagagc gcggtatcat ccacgtcggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa actcaaaaaa ccacttgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcaa gcaggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caagtattgg ctaaaccggg tacaatcact cctcacacca agttcaaagc
      901 agaagtatac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaat
//
LOCUS       DAWKQB010000234          973 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.235, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000234 DAWKQB010000000
VERSION     DAWKQB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.235"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            57..782
                     /locus_tag="WJL14_002040"
     CDS             57..782
                     /locus_tag="WJL14_002040"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ7657051.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFDAGFQTVTLGKR
                     ILRTETVALAALAAMQTLWGDFA"
BASE COUNT          261 a          337 c          215 g          160 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcta cctgcccgaa aacctttccg tcggacaaac cgtcgccctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctacgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacagctga ccgttttgga aaaacgccgc gccgaagccg
      241 aaatcctgca cgaagacaca accgacaacg agtccccgct caacatcaca ctgatacaat
      301 ccatctcctc cggcgatcgc atggatttca ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacgga gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 acccgtccgg cgcaatcgtc tttatggtcg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgatgcc ggcttccaaa ccgtaacatt gggcaaacgc atcctgcgca
      721 ccgaaaccgt cgccctcgcc gccctcgccg cgatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttccccc gccgtcattc ccgcgaaagc aagaatctag aaacgaaaaa
      901 ctacagggat ttatccgaaa caacaaaccc tctccgccgt cattcccgcg caggcgggaa
      961 tctagaaatt taa
//
LOCUS       DAWKQB010000235          906 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.236, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000235 DAWKQB010000000
VERSION     DAWKQB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.236"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            34..>906
                     /locus_tag="WJL14_002041"
     CDS             34..>906
                     /locus_tag="WJL14_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ7657052.1"
                     /translation="MNGQKLPEVKWGQSYNSLPQKSNPRQVNFTTSFSGYKKNTTFSF
                     NNTDEVVAQKNGVTVFGAATYLPPYGKVSGFDEKRLKERGDVLGWIGTTRPGLVGYSY
                     EGNTCSSGDCPDVSYKTQFTFGNQGLKSKVNGKLDIYEDKSRDNSPIYKLPDQPWLGV
                     SFNLSSESVVESKKLKKVESSFSEDVTQSNGAQSLYKYKNLVYTTGDVRNKGNQVHQD
                     KHHAVAFYLNAKLHLLDKKNIQNIAQGITVNFGTLKTRIEPTDAWKNKRHLTHNVGNW
                     EFKDTGSVSVKLKLP"
BASE COUNT          284 a          204 c          213 g          205 t
ORIGIN      
        1 gcgcaaacgc aaacgtatcc atacgctctt gtgatgaacg gacaaaagct gcccgaggta
       61 aagtgggggc aatcatataa ttcattgccg caaaaaagca acccccgcca agttaacttt
      121 acgacatctt ttagtggata caaaaaaaac actactttct cattcaataa taccgatgaa
      181 gttgttgctc aaaaaaacgg tgttaccgtt ttcggcgcgg cgacctacct gccgccctac
      241 ggcaaggttt ccggttttga tgaaaagagg ctgaaagagc gcggcgatgt ccttggttgg
      301 attggtacga cccgcccggg gttggtaggc tacagctacg aaggtaacac atgctccagc
      361 ggagactgtc ctgatgttag ctataaaacc caatttacct tcggtaatca agggttgaaa
      421 agcaaggtaa acggcaagct ggatatatac gaagacaaaa gccgcgacaa ttcgcccatt
      481 tataaattgc cggatcagcc ttggttgggc gtgtctttca atttaagcag cgagagcgtc
      541 gtcgaatcaa agaaactcaa gaaagtggaa tcttctttta gcgaggatgt gacgcagagc
      601 aatggtgcgc aaagcctata caaatacaaa aacctcgttt atacgacagg cgatgttcgt
      661 aataagggta atcaagttca tcaggacaaa caccacgctg tcgcctttta tctgaacgcc
      721 aagctgcact tgctggataa aaaaaacatc caaaatatcg cgcaaggcat aacagttaat
      781 tttggtacct tgaaaacacg catcgagccg acggacgcgt ggaagaacaa gaggcatctc
      841 actcataatg ttggtaattg ggagttcaaa gataccggat cagtcagcgt caaactcaaa
      901 ttgccg
//
LOCUS       DAWKQB010000236          902 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.237, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000236 DAWKQB010000000
VERSION     DAWKQB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.237"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJL14_002042"
     CDS             complement(85..717)
                     /gene="mtrR"
                     /locus_tag="WJL14_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ7657053.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          201 a          200 c          236 g          265 t
ORIGIN      
        1 tatttttaga agaaggcttt gtgtcatttt gatgccgtct gaaagggttt gttcgtttca
       61 gacggcattg ttaccaaggc ttgattattt ccggcgcagg tcgggatggt tttccaagtt
      121 gtccatcatt atcccgatga tgcgcggggc ggttttgccc aaatcgaaac gttcgcagga
      181 agagaaccac cgccaaatca gcccgtccaa ggttgatttg atgaagataa ctgccgtttc
      241 cttgtccaaa tcgtcagcca aatcctgatt ttccaccgct tcggtcaaaa cggcggtaat
      301 tttctcgcgc cagattgcct gatgcttgcg ggcaatggcg ataacggcgg cgttttgctc
      361 cgtgtgttcg cattttaaaa acaggatgtt gtggaatttg tagtagatgt cgttgctttg
      421 cagccgctcg aaaaagtgca gcagcgtgtg gcggaatacc gcccaagacc ctccttcggc
      481 atcttcggta tcttgcgcga tgcagttttc gatgtcgtcg cagatacgtt ggaacagcgc
      541 gtcgaacaag tcttccttat ttttgaaatg ccaatagagc gcgccgcgcg ttacgccggc
      601 ggcttgggcg atttcgttga gcgaggtgcg cgcaatccct ttgcggtaaa aggtttccaa
      661 ggcggcaagc atcaggtgtt ctttggtttt caaggcttcg gttttggttt ttctcataat
      721 ggcgttttcg tttcgggtcg gtttgatgag ggcggattat aaaaaagact ttgtaaccat
      781 gcaatcgtgt atgtataatg aaacccatga aattgagact acatctcaac tttgaaaaca
      841 ggcataacct gctttgcaac ccgttcgaac attcgggtat caaagcaggc ggtttttata
      901 tc
//
LOCUS       DAWKQB010000237          874 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.238, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000237 DAWKQB010000000
VERSION     DAWKQB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.238"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(55..834)
                     /locus_tag="WJL14_002043"
     CDS             complement(55..834)
                     /locus_tag="WJL14_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ7657054.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          205 a          289 c          172 g          208 t
ORIGIN      
        1 aatttagttc actataccga tacatccgaa tatgccagac cgtctaaacg gtattcatac
       61 ctcccaatcc tgcacgcgct tcaaatcgtt ttgcgccaaa gtatccgcgt ggcggttacg
      121 gctctgatat tccctgtctt tcaatatact gctcgccaca taattcaaat gcgcctttgc
      181 cgcctccgaa gcctcgcccg gccgacggtt cgatatcgcc tcatacagca cgcggtgctg
      241 cgccatcagt ttcggacgcg gatcttcttc ctgattcaga taaataaggc tgctgcgcgt
      301 ctgccggtac agcattttca acaaaccgcc cgacaaatgg ctgaacaaca aattgtgcgc
      361 cgcatccgca atcgtctgat ggaagctgac atcggcttcg ctctgatgtt ccagattgcc
      421 gctttcgcac gcctcctcaa acttttcaag ccaaaaccca atccgcttca aatcggcatc
      481 cgtccggcgt tctgccgcca atgccgccat acagccctcg atgtggcagc tgaaatcaaa
      541 aacatcctgt tcccaattgg aatgcttgcc caaaagctcc tgccaacttt gcaaaaaatc
      601 ctgctgtggc ttgaccgaaa cataataacc gtctccctgc ctcgcttcca aaacctgacg
      661 ggcgaccaaa acattcaatg ccgacctgac cgacgggcgg gacacgccga actcttccgc
      721 caaaacgcgt tcgggcggaa tcttgccccc ttccgcgtaa accccttcca cgatgcggcc
      781 ttccaatacc gacaatacct gatcgctgat tttctgaggc cttaccagtt tcatcactcc
      841 tcctttataa agattccctg ccgaaccctt ccga
//
LOCUS       DAWKQB010000238          766 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.239, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000238 DAWKQB010000000
VERSION     DAWKQB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.239"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            244..543
                     /locus_tag="WJL14_002044"
     CDS             244..543
                     /locus_tag="WJL14_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ7657055.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPASMAVSSLPPQPH"
     gene            483..719
                     /locus_tag="WJL14_002045"
     CDS             483..719
                     /locus_tag="WJL14_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ7657056.1"
                     /translation="MRLRDAGEYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRTMPDIHVYGTPRSLTATVKHIVD"
BASE COUNT          214 a          213 c          159 g          180 t
ORIGIN      
        1 atccactata catcaaacat caaattggac tgaccaatca gggcggattc taatgacacg
       61 ccgttcccgc cgtcaacggc atttacctcg caccgccccc gaaacacaag aaaaaactac
      121 accaaactac aatttttgtt catgcaaata tttgttttga caggatttaa acgaaagctc
      181 cgattcaaat ctgccgaacc gcccaaaaat atattgacct aaatattaaa gtttcgtaaa
      241 gtaatgcaac gttgctttaa ttggtttgac cactattgcc gacgattaga aaaatatttt
      301 cggagatgtt caattatgga aacttgggtt caaaactaca cggcaatcgg cggcagcctg
      361 tatctgactg ccgccgccgc actcttaccc atcgtctttt tctttgccgc gctgaccgtc
      421 ctgaagctga aaggctatca ggcggggctt tatacgctgc tgattgcgct tgccgttgcc
      481 gtattcggct tcgggatgcc ggcgagtatg gcggtttctt ccctgccgcc gcagccgcac
      541 tgacccaacg cccctacgcc acgctggatt tgaccgcgca ttacaaaatc ggaaaatcca
      601 cacgcatcgg tttggacttt gaaaacgtgt tcaacaaacg ctaccgcacc atgcccgaca
      661 ttcacgttta cggcacgccg cgcagcctga ccgcaaccgt caaacatata gtggattaac
      721 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtac
//
LOCUS       DAWKQB010000239          718 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.240, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000239 DAWKQB010000000
VERSION     DAWKQB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.240"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            147..>718
                     /locus_tag="WJL14_002046"
     CDS             147..>718
                     /locus_tag="WJL14_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ7657057.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETK"
BASE COUNT          166 a          203 c          207 g          142 t
ORIGIN      
        1 tgcccgcctg tgagtggcgg ggcagggaat ctgtgtgcgg attatgccat aaaacggtgt
       61 ccgacccaat cgcgggcgcg tccggagatt ggaaatcctg cttaaaaaat gtacaatggc
      121 gtactttttt gaaacgcgga tccattatgc acatcggcgg ctattttatc gacaacccca
      181 tcgcacttgc gccgatggcg ggcattaccg acaaaccgtt ccgccgactt tgccgagatt
      241 ttggcgcagg ttgggcggtg tgcgaaatgc tgaccagcga cccgacgctc agaaatacta
      301 gaaaaacctt gcaccgcagc gattttgccg atgaaggcgg cattgttgcc gtgcagattg
      361 ccggaagcga tccgcagcag atggcggatg ccgcgcgtta caacgtcagc cttggggcgc
      421 agcttatcga catcaacatg ggctgtcccg ctaaaaaagt ctgcaatgtc caagccggta
      481 gcgcgctgat gcagaacgag ccgctggttg ccgccatttt ggaggcggtg gtcaaagcgg
      541 cgggcgtacc cgttaccctc aaaacccgtt tgggttggca cgacgaccat caaaacctgc
      601 ccgtcatcgc caaaatcgcc gaagattgcg gcattgccgc ccttgccgtc cacggacgca
      661 cgcgcacgca aatgtacaaa ggcgaagcgg cttacgacct gattgccgaa accaaatg
//
LOCUS       DAWKQB010000240          695 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.241, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000240 DAWKQB010000000
VERSION     DAWKQB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.241"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..572
                     /locus_tag="WJL14_002047"
     CDS             <1..572
                     /locus_tag="WJL14_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ7657058.1"
                     /translation="HSIHPRVSVGYDFGDWRIAADYASYRKWNDNKYSVNTKNVQVNK
                     SNGNRQDLKTENQENGTFHAVSSLGLSAVYDFNTGSRFKPYAGVRVAYGHVRHSIDST
                     KKTTNVLTVPTNIPGGTPTIYNQGSTQDAYHESHSIRRLGLGVVAGVGFDITPKLTLD
                     TGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          187 a          204 c          160 g          144 t
ORIGIN      
        1 cgcattccat ccaccctcgg gtgtcggtcg gctacgattt cggcgactgg aggatagcgg
       61 cagattatgc cagttacaga aaatggaacg acaataaata ttccgtcaac acaaaaaatg
      121 tgcaggtgaa taaaagcaat ggcaacaggc aagacctgaa gacggaaaat caggaaaacg
      181 gtacattcca cgccgtctcc tcgctcggct tgtccgccgt ttacgatttc aataccggtt
      241 cccgcttcaa accctatgca ggcgtgcgcg tcgcctacgg acatgttaga cacagcatcg
      301 attcgaccaa aaaaacaaca aatgttctta ccgtccccac caatattcct ggcggaacac
      361 ctacgattta taatcaggga agtacgcaag acgcctatca cgaaagccac agcatccgcc
      421 gcttgggtct tggtgtcgtc gccggtgtcg gtttcgacat cacgcccaag ctgactttag
      481 acaccggata ccgctaccac aactggggac gcttggaaaa cacccgcttc aaaacccacg
      541 aagtctcatt gggcgtgcgc taccgcttct gattccccga caccgatgcc gtctgaacct
      601 tcagacggca tttttgattc acctgccgtt tacagacgcg gggcgggcgt ggaaataccc
      661 gaaccgtcat tcccgacaac accgtaatct tgaaa
//
LOCUS       DAWKQB010000241          677 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.242, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000241 DAWKQB010000000
VERSION     DAWKQB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.242"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(91..642)
                     /locus_tag="WJL14_002048"
     CDS             complement(91..642)
                     /locus_tag="WJL14_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657059.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          187 a          272 c          127 g           91 t
ORIGIN      
        1 caaagaaacc gccattagca ctacaccccc ccaagctgcg gctgccaaac acatcggctg
       61 cttctcgacg cacccgctga aaaatgcgtc ctatccgtcc gccttgcccg caccccctga
      121 ctttgccgcc ctgcccgatg ccgtctggag cctgaaccgc cccaccagcc cgatgacgca
      181 cgacaccgcc ggaaagggca tacacaaagc cgtcagcaca taacgcgcat tccccgtatt
      241 gaccacgtcc gcgcccttca gcagcagcgg cagcgcgttc gcccactccc acaccgacgg
      301 cgcgctgaac gccaataccg ccaccagccc caacatccgg aacaccccga tttccaacac
      361 ctgccgccgc cgcaccgaac ggttgagcaa aaacacaacc gccaacccga aacagaccac
      421 cgccgccata ccgccgttgg cgacaatctg caaactgttg aatgcaatat cgggcgtaac
      481 cgccaacacg ccttccgaat ccggcacggc acgctgcaaa tccaaaccct gcaacacatt
      541 caacacaaaa ccgtagatca aatcaaaaaa caccacgcct atcggcaaag cactcaaaac
      601 ccgccacatc ttcctgtccg accaaccgtt caaaactttc attttacccg accacgcaag
      661 ccgccgaaca aaaaaca
//
LOCUS       DAWKQB010000242          660 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.243, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000242 DAWKQB010000000
VERSION     DAWKQB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.243"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(59..544)
                     /gene="fxsA"
                     /locus_tag="WJL14_002049"
     CDS             complement(59..544)
                     /gene="fxsA"
                     /locus_tag="WJL14_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ7657060.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRFRNAIEHKKD
                     E"
BASE COUNT          194 a          210 c          121 g          135 t
ORIGIN      
        1 ctatattttc aaaccggaaa aatgccgtct gaaccttcag acggcattcc attcacattt
       61 attcgtcttt tttgtgttcg atggcgtttc ggaaacgatt gccgccgtaa ggctcttcaa
      121 ccgtatattc cccctcgata atatcgtcat cgcgggaaaa gccctctttt ctgcccgatt
      181 ggttcatgtt gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct cccttaaacg
      241 gcagcagcag caataccgcc aacaccgagg atacgaatcc cggactcatc agacacaccg
      301 ccgccaccgt ataacggata ggccacaaca tctgataaac ggataccctc ccgccgcttc
      361 tcattgccgc gcccgccaat aaaagaccgg acagccccgt atgcctgagc atcagcacgc
      421 cggcggcaaa ggttgccgcc attagaaaca gcgtccaacc gccgcccaac caatcggcaa
      481 cccacacaat cgacataatc tccaaaaaca gcagcaccaa aaaaccgata ccgaaaaatc
      541 tcattgaccg tcatccttat atttaagtaa acagcaaacc gcccgaacag gactccaagc
      601 gagctgcctg taaatgatta caaaaccatg tgcttcaagc cgaaacaatg tgaaatcacg
//
LOCUS       DAWKQB010000243          609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.244, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000243 DAWKQB010000000
VERSION     DAWKQB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.244"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
BASE COUNT          158 a          170 c          119 g          162 t
ORIGIN      
        1 caacgccgta ctggtttttg ttaatccact ataccgtcgt tcccacggtc aggatttcag
       61 attgcggaca tctgtcagaa aagacaaaaa accttccgcc gtcattccct acaggcgaga
      121 atccgatccg ttgaaattcg gttgttttaa ataaattctt acagctttga ttttctgttt
      181 ttccgataac gccgtaactt tgaaacgcga aagcggtaat ccgatccgtt gggattttgc
      241 aacttcaaat caatccgcaa actgaaatcc cgtcattccc acgcagtcgt gaatccgaac
      301 gcgtccgcac ggaaacctgc atcccgtcat tcccacgaaa gtgggaatcc agttttttga
      361 gtttcagtca ttcccgataa attgccttaa cattgaatgt ctagattccc gcctgcgcgg
      421 gaatgacgaa tccatccgca cggaaacctg caccgcgtca ttcccacgaa cctacattcc
      481 gtcattccca cgaaagcggg aatccagttt tttgagtttc agtcattccc gataaattgc
      541 cttagcattg aatgtctaga ttcccgcctg cgcgggaata acgaatccat ccgcacggaa
      601 acctgcacc
//
LOCUS       DAWKQB010000244          609 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.245, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000244 DAWKQB010000000
VERSION     DAWKQB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.245"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            208..501
                     /locus_tag="WJL14_002050"
     CDS             208..501
                     /locus_tag="WJL14_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657061.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKEQIALFPRGLNGITKLPAAVLLPERPYHFDMKEVLHIFSWIPR"
BASE COUNT          159 a          157 c          152 g          141 t
ORIGIN      
        1 tcgccttgtc ctgatttttg ttaatccact atattgtcca ttcggcaggg aaagcctgaa
       61 gaagaatttt cccgcaagat ggccggctgc ctaaccgact tggaaaactg tttggaaaaa
      121 tacctcgaac agttcgggcc ggtatcggaa agtatcgaag cctgtaccgt caaactgcag
      181 gagtaaccgt cgttcttcaa caggctgatg aaaaccaacg acaagctcaa taggcagatt
      241 gacgtgttgc agaaacagtc ggcagccatc cacaacgaag cctatatcga aatgaacacg
      301 ctgctttacc gccaccgcga agtggtttcc gtccacaacc gcaaagcaga ttatgcggaa
      361 aagggcaaag agcagattgc gctgttcccg cgcggattga acggcatcac caagctgccc
      421 gccgccgtcc tgttgcccga gcgtccctac cattttgata tgaaggaggt tctgcatata
      481 ttctcctgga taccgaggta ggcgtatcgg ctgccggata aaaatgcacc ccgtcattcc
      541 tgtctgtacg ggggtagcgg cagtgttttc ctgcttcgtt ttatagtgga ttaacaaaaa
      601 ccagtacgg
//
LOCUS       DAWKQB010000245          572 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.246, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000245 DAWKQB010000000
VERSION     DAWKQB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.246"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..382
                     /locus_tag="WJL14_002051"
     CDS             <1..382
                     /locus_tag="WJL14_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ7657062.1"
                     /translation="LERLAARDIIRFPGFVNNVSEVIKEHHIFVLPSYYREGVPRSTQ
                     EAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAIRLMGNA
                     SYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          139 a          137 c          140 g          156 t
ORIGIN      
        1 tttggaacgg cttgccgccc gcgatattat ccgtttcccc ggttttgtga acaatgtttc
       61 cgaagtgata aaagaacatc atatattcgt attgccgtct tattataggg aaggcgttcc
      121 ccgaagcact caggaggcaa tggccgtcgg cagggcagtg attacgacgg atgtccccgg
      181 atgcagggaa acggtcgccg acaaggtcaa cggcttcctg atcgaacctt ggaatccccg
      241 catcttggcc gaaaaaatga tttattttat cgaaaacagg gaagccatcc gcctgatggg
      301 gaatgcaagt tatgcgattg ccaaagataa attcgatgcc gaaaaagtcg atttgaaatt
      361 actcgatatt ttgaaggcgt aaacaaggct gcccgctttt gagtttcggt catttctgat
      421 aaacccccgt cattcccgcc acacccccgt cattccctcg aaagcgggaa ttcaggttcg
      481 tttagtttcg gtcatttccg ataaattctt gcaactttgc gtttctagat tcccactttc
      541 gtgggaatga cggcggaggg ttgctgtttt cc
//
LOCUS       DAWKQB010000246          560 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.247, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000246 DAWKQB010000000
VERSION     DAWKQB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.247"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..174
                     /locus_tag="WJL14_002052"
     CDS             <1..174
                     /locus_tag="WJL14_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ7657063.1"
                     /translation="NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTP
                     SQQLKILWDLGLI"
BASE COUNT          128 a          119 c          150 g          163 t
ORIGIN      
        1 aaccacatta acggcattga gaatttttgg aatcaggcaa aacgtgtctt gcgaaaatac
       61 aatggaatcg atcgtaaatc tttcccgctg ttcttgaaag aatgcgaatt tcgatttaac
      121 ttcggcacac cgtctcaaca gcttaaaatc ctgtgggatt tagggctaat ctagtacagc
      181 cccttagatt taattcacta tacctgcgta atggtatcgg gtactcatca tcaacctgcc
      241 ttgcattcgg ttgtgttgtc cggcaactgt ccggttgtgt cgatgggttg ccctgccgct
      301 ccctgtgctg cgcaacggtt gtgtgccttg ggtgggcgcg ggtggcggcc gggcggtttg
      361 ttgctatgat acgggagttc cgatatacaa gggtgggctt cagcccacca atcaacgacc
      421 gaaaaagaaa aaacgttggg ctgaagccca ctctacgcaa ttacgcaact gttatcgtgg
      481 ggatgacggg attttaggtt tttgtttttc tgtttttgcg ggaaggatgg gttttaggtt
      541 gcggtcattt atcggaaaag
//
LOCUS       DAWKQB010000247          553 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.248, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000247 DAWKQB010000000
VERSION     DAWKQB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.248"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            59..346
                     /locus_tag="WJL14_002053"
     CDS             59..346
                     /locus_tag="WJL14_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ7657064.1"
                     /translation="MDDLIAAFLGCLSGKYSEGKISRLEISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITGIMKDLFLFSCSVCLLFYFLLFIAETIRKTINKIK"
BASE COUNT          187 a           62 c           89 g          215 t
ORIGIN      
        1 taatggcaaa acaggtaatg aattaaagac attacctgtt tttacagaaa tttttaaaat
       61 ggatgatttg attgcagctt ttttaggctg tttatcaggt aaatatagcg aagggaaaat
      121 aagcagatta gaaatatctc taacggcagg aagcattttc tttgcagtat tctttattca
      181 tgaactgata atttttattg ttgatggaaa taaggaaaat ctttggaatt ttattactgg
      241 gataatgaaa gatttatttt tattttcatg ttctgtttgt ttattattct acttcttatt
      301 atttattgcg gaaactatta ggaaaacaat caataaaatt aaataagata tttaagccgc
      361 taaaaaccat ttaagtattt tatcttttct atgcggcatt tatcttggaa ttgaaatact
      421 gcaatcggga ggctaaggct tccgttgtaa cgtttaccat atatttcatt attttaattt
      481 catcaaaaaa agaaattcaa gattatacat actgccaata gtttcttgat tgttttgtat
      541 agtggagtaa att
//
LOCUS       DAWKQB010000248          545 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.249, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000248 DAWKQB010000000
VERSION     DAWKQB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.249"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(104..419)
                     /locus_tag="WJL14_002054"
                     /pseudo
     CDS             complement(104..419)
                     /locus_tag="WJL14_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
BASE COUNT          141 a          141 c          142 g          121 t
ORIGIN      
        1 cgctgtaaaa ttgccgccgc tccgcattca gacggcatcg gcaataaaaa agcccgaaaa
       61 aatcgggctg aaagttaagg ctgcatcgcc tttaaagcgg ctttcaccaa aaaaccgctc
      121 gggtttcatg acggcggcag tgcaggcatc gacacggttg aggatatgtt gcggccaact
      181 gatattaaac cgtacctgtc cggttaatgc ggcatcgtcc gcttcaacca tcgtccacaa
      241 caccgctagg cataatcggg attgtcccga tgaaccttta tgctttgcgc cccaggaatc
      301 ggcaggtttt cctgcaaaag cccttcgaga tggaacattg cggcggcttt ggtatcggca
      361 agcgcgtcgg caaccgtatc gccgacagga tagcagcccg gcagatcggg aataatcacg
      421 cctcagttgc tttaatcggg gtcttggaac agcacagcgg gatagtaaat catcgcttcc
      481 tttctcggat atgcggtatc ggcaaagtcc aaacgcaacg aaggggattt atagtgaatt
      541 ataat
//
LOCUS       DAWKQB010000249          539 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.250, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000249 DAWKQB010000000
VERSION     DAWKQB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.250"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>539
                     /locus_tag="WJL14_002055"
     CDS             <1..>539
                     /locus_tag="WJL14_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ7657065.1"
                     /translation="DELSIPDYSTLCRYRNRLAQDDTLSELLELINRQLTEKGLKVEK
                     ASAAVVDATIIQTAGSKQRQAIEVDNKGQVSGQTTPSKDGDARWTKKNGLYRLGYKQH
                     TRTDAEGYIEKLHITPANTHECNHLLPLLEGITEGTTIYADKGYDSAENRTSGRTSVV
                     RRHYARAAYFGLIKVSAQS"
BASE COUNT          169 a          149 c          129 g           92 t
ORIGIN      
        1 gacgaactga gcatccccga ttacagcacc ttatgccgct accgcaaccg gctggcgcaa
       61 gacgacaccc tgtccgaatt gctggaactg attaaccgcc aactgaccga aaaaggttta
      121 aaagtagaga aagcatccgc cgccgtcgtt gatgccacca ttattcagac cgccggcagc
      181 aaacagcgtc aggccataga agtcgataac aaaggacaag tcagcggaca aactacaccg
      241 agtaaagacg gcgatgcccg ttggacaaag aaaaacggcc tctacagact cggttacaaa
      301 caacataccc gtaccgatgc ggaaggctat atcgagaaac tgcacatcac tccagccaat
      361 acccatgagt gcaaccacct gttgcctttg ctggaaggca ttaccgaagg cacgaccatc
      421 tatgccgaca aaggctatga cagtgcggaa aaccgaacat ctggaagaac atcagttgtt
      481 agacggcatt atgcgcgggc agcctatttc ggactgatta aagtgagtgc gcaaagcca
//
LOCUS       DAWKQB010000250          531 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.251, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000250 DAWKQB010000000
VERSION     DAWKQB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.251"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            202..486
                     /locus_tag="WJL14_002056"
     CDS             202..486
                     /locus_tag="WJL14_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ7657066.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTLRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          158 a           80 c          139 g          154 t
ORIGIN      
        1 aggcgggaat ctaggtttgt cggtgcggaa acttatcggg taaaacggtt tctttagatt
       61 ttgcgttcta gattcccact ttcgtgggaa tgacgaagag ttgcgggaat gatggaaagc
      121 tatgggaata acgaaaggtt aaagtaatca cgggatggtg ttcgcgggaa tataaattta
      181 aataattcaa aagggtatta tatgcagcct gcggtttata ttttagcaag ccaacgtaat
      241 ggcacgttat acattggcgt tacatctgat ttggtgcaac gtatttacca acatagggag
      301 catttgattg agggatttac attacggtac aacgttacta tgctggtttg gtatgaactg
      361 catcctacga tggagagtgc aattactcgg gaaaaacagt tgaagaaatg gaacagggct
      421 tggaaattgc aactgattga agaaaataat gtttcttggc aggatttatg gtttgatatt
      481 atttagcccg tcattcccgc gcaggcggga atctagacct tagaacaaca g
//
LOCUS       DAWKQB010000251          530 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.denovo.252, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000251 DAWKQB010000000
VERSION     DAWKQB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.denovo.252"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            <1..>530
                     /locus_tag="WJL14_002057"
     CDS             <1..>530
                     /locus_tag="WJL14_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ7657067.1"
                     /translation="IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKL
                     DSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGG
                     ATIRAGVGEQARADAKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFT
                     GPVAPVLSAPGGYIVD"
BASE COUNT          159 a          159 c          128 g           84 t
ORIGIN      
        1 atcgacatca aatctggtgg cagcatcgac gcctacgcca ccgcattcga tgcccccaaa
       61 ggcagcatta acatcgaagc cgggcggaaa ttgacactct atgccgtaga agagctcaac
      121 tacgacaaac tagacagcca aaaaaggcgc agatttctcg gcatcagcta cagcaaagca
      181 cacgacacca ccacccaagt catgaaaacc gcgctgccct caagggtagt tgcagaatca
      241 gccaacctcc aatcgggctg ggataccaaa ctgcaaggca cacagtttga aaccacactg
      301 ggtggcgcaa ccatacgcgc aggcgtaggt gagcaggcac gggcagatgc caagattatc
      361 ctcgaaggca tcaaaaccac gatccacaac gaaaccgtga gcagcagcaa atctgctcta
      421 tggcaaaaac aggcaggacg gggcagtaac atcgaaacct tgcaattgcc gagtttcacc
      481 ggtcccgttg cgcccgtact gtccgcaccc ggcggttaca ttgtcgatat
//
LOCUS       DAWKQB010000252         5064 bp    DNA     linear   BCT 20-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate M22688_Neisseria
            meningitidis SAMN10275887-rid8981033.guided.145, whole genome
            shotgun sequence.
ACCESSION   DAWKQB010000252 DAWKQB010000000
VERSION     DAWKQB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN10275887
            Sequence Read Archive: SRR8200282
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-FEB-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000676274.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000676274.1
            Genome Coverage       :: 149x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/21/2022 17:44:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,061
            CDSs (total)                      :: 2,006
            Genes (coding)                    :: 1,935
            CDSs (with protein)               :: 1,935
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 47
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 37 of 71
            Pseudo Genes (incomplete)         :: 34 of 71
            Pseudo Genes (internal stop)      :: 8 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN10275887-rid8981033.guided.145"
                     /isolate="M22688_Neisseria meningitidis"
                     /isolation_source="CSF"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /collection_date="2000"
                     /collected_by="Centers for Disease Control and Prevention"
     gene            complement(369..1883)
                     /gene="katA"
                     /locus_tag="WJL14_002058"
     CDS             complement(369..1883)
                     /gene="katA"
                     /locus_tag="WJL14_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ7657068.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQAGLL"
     gene            complement(2146..2763)
                     /gene="tetR(B)"
                     /locus_tag="WJL14_002059"
     CDS             complement(2146..2763)
                     /gene="tetR(B)"
                     /locus_tag="WJL14_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HEZ7657069.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLNGFM"
     gene            2845..4050
                     /gene="tet(B)"
                     /locus_tag="WJL14_002060"
     CDS             2845..4050
                     /gene="tet(B)"
                     /locus_tag="WJL14_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HEZ7657070.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIVGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            4147..4998
                     /locus_tag="WJL14_002061"
     CDS             4147..4998
                     /locus_tag="WJL14_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ7657071.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVEDWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEEDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAVGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
BASE COUNT         1145 a         1194 c         1320 g         1405 t
ORIGIN      
        1 gctgtggggc gggaatggcg ggctgtaggg tgggcttcag cccaccgcct ccgccaactt
       61 cagacggcat aggtttgccg gcaccggtaa gattgtcgga tttgtcggat ttgtcgaggt
      121 ggcggatttg tcgaggtggc ggattggtgt gattggtgtg attggtgtga ttggtgggct
      181 gaagcccacc ctacgaacac cctacgtccc actgcgatgc gcgaccatat aaaaatgcgg
      241 ggtggggtgt ggaaatgccg tctgaagcgg tcaaatgacg tttcagacgg catttttatg
      301 cccggttatt tccggtagcg gacggcgcgg gacaggattt cttcaatttc catccacata
      361 atgccccctt acagcaaacc ggcctgaccc agtgcggggt cggtcgcgcg ggcggcttgt
      421 gcgtcttcga cagtcagtcc gagggctttt gccacgcctt cgccgtatgc cggatcgcaa
      481 cggtagcagt tgcggatatg gcggtatttg atgaagtcgg gcgcgtcgcc cattgcggcg
      541 gcggtgttgc cgaacaatgc ctgtttctgc gcgtcgttca tcaggttgaa cagggcgcgc
      601 ggttggctga aatagtcgtc atcgtcttgg cggtagtccc agtgcgccgc gtcgccgttg
      661 attttcaaag gcggttcggc gaagtcgggc tgctgctgcc attggccgaa gctgttgggt
      721 tcgtagtgcg gcaggctgcc gtagttgccg tcggcgcgtc cttgtccgtc gcgctggttg
      781 ctgtgaacag ggcaacgcgg acggttgaca gggatttggc ggaagttcac gcccaagcgg
      841 tagcgttgcg cgtcggcgta attgaacaaa cgcgcctgca acattttatc tgggctggcg
      901 ccgacaccgg gaacgaggtt gctcggtgcg aaggcggatt gttccacatc ggcgaagaag
      961 ttttcgggat tgcggttcaa ctcgaattcg cccacttcaa tcagcggata gtcttttttc
     1021 ggccaaactt tggtcaagtc aaacggatga tacggcactt tttccgcatc ggcttcaggc
     1081 atgacttgga tgtacatcgt ccatttcgga aactcgccgc gctcgatggc ttcgtacagg
     1141 tcgcgctgat ggctttcacg gtcgtcggcg atgattttgg cggcttcttc gttggtcagg
     1201 tttttaatgc cttgttgggt gcggaaatgg aatttcaccc aaaaacgctc gcctgcttcg
     1261 ttccagaagc tgtaggtatg cgaaccgaag ccgtgcatat ggcggtagcc ggcggggatg
     1321 ccgcggtcgc tcatcacgat ggtaacttgg tgcagtgctt cgggcagcag cgtccagaag
     1381 tcccagttgt ttgtggcaga gcgcatattg gtgcgcgggt cgcgtttgac ggctttgttc
     1441 aggtcgggga acttacgcgg gtcgcgcagg aagaacacgg gcgtgttgtt gccgaccaca
     1501 tcccagttgc cttcttcggt atagaacttc aacgcaaaac cgcggatgtc gcgttctgca
     1561 tcggctgcgc cgcgttcgcc tgccacggtg gtgaaacggg cgaacatctc ggtttttttg
     1621 ccgacttcgc tgaagatttt ggcgcgggtg tatttggtga tgtcgtgcgt tacggtaaac
     1681 gtaccgaacg cgcccgaacc tttggcgtgc atacggcgtt cggggatgac ttcgcgcacg
     1741 aagtcggcga gtttttcatt cagccacaaa tcctgcgcca gcagagggcc gcgaggaccg
     1801 gcggtcaggc tgttttgatt gtcggcaaca ggcgcgccgt tgttcatggt cagatgggtt
     1861 acggggcatt tggaggtagt catagctcgt gttccttttc cttggttaat caaaagatac
     1921 gacaaacggc ttacagtacg atttggcgga aagcgtattc gtaaccggtt tcttgattgc
     1981 aataaatttc ttgaatcgac attttgtttt ccttttgtaa aaactatgga tgagactata
     2041 cgctaagatt tttgctatta aaactatgaa atcgatttaa tattgttata agcaatcggt
     2101 ttttgatttt cgtttgtttt ttgttatcga aacgaatcaa aatccctaca taaaaccatt
     2161 aagttgtttt tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc
     2221 accttggtga tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt
     2281 aggtgtttcc ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa
     2341 agtaaaatgc cccacagcgc tgagtgcata taatgcattc tctagtgaaa aaccttgttg
     2401 gcataaaaag gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc
     2461 taaatgtact tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt
     2521 attacgtaaa aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct
     2581 atctaacatc tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata
     2641 caatgtaggc tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat
     2701 tccgacctca ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga
     2761 catcattaat tcctaatttt tgttgacact ctatcattga tagagttatt ttaccactcc
     2821 ctatcagtga tggagaaaag tgaaatgaat agttcgacaa agatcgcatt ggtaattacg
     2881 ttactcgatg ccatggggat tggccttatc atgccagtct tgccaacgtt attacgtgaa
     2941 tttattgctt cggaagatat cgctaaccac tttggcgtat tgcttgcact ttatgcgtta
     3001 atgcaggtta tctttgctcc ttggcttgga aaaatgtctg accgatttgg tcggcgccca
     3061 gtgctgttgt tgtcattaat aggcgcatcg ctggattact tattgctggc tttttcaagt
     3121 gcgctttgga tgctgtattt aggccgtttg ctttcaggga tcacaggagc tactggggct
     3181 gtcgcggcat cggtcattgc cgataccacc tcagcttctc aacgcgtgaa gtggttcggt
     3241 tggttagggg caagttttgg gcttggttta atagtggggc ctattattgg tggttttgca
     3301 ggagagattt caccgcatag tccctttttt atcgctgcgt tgctaaatat tgtcactttc
     3361 cttgtggtta tgttttggtt ccgtgaaacc aaaaatacac gtgataatac agataccgaa
     3421 gtaggggttg agacgcaatc gaattcggta tacatcactt tatttaaaac gatgcccatt
     3481 ttgttgatta tttatttttc agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg
     3541 ctatttaccg aaaatcgttt tggatggaat agcatgatgg ttggcttttc attagcgggt
     3601 cttggtcttt tacactcagt attccaagcc tttgtggcag gaagaatagc cactaaatgg
     3661 ggcgaaaaaa cggcagtact gctcggattt attgcagata gtagtgcatt tgccttttta
     3721 gcgtttatat ctgaaggttg gttagttttc cctgttttaa ttttattggc tggtggtggg
     3781 atcgctttac ctgcattaca gggagtgatg tctatccaaa caaagagtca tcagcaaggt
     3841 gctttacagg gattattggt gagccttacc aatgcaaccg gtgttattgg cccattactg
     3901 tttgctgtta tttataatca ttcactacca atttgggatg gctggatttg gattattggt
     3961 ttagcgtttt actgtattat tatcctgcta tcgatgacct tcatgttaac ccctcaagct
     4021 caggggagta aacaggagac aagtgcttag ttatttcgtc accaaatgat gttattccgc
     4081 ttataatgat gtttcccggc atcatctgcc gccgacaggc caaaagaata attctctcgc
     4141 attatcatga cgttactcgg aataaagctc aaacagaccc agcagctcaa ccagcggctg
     4201 caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac ttgaacgcga ggtcgaagac
     4261 tggctgtcgg acaaccccct gctcgaacgc aaagacacgg atgaattttc cgatgccgaa
     4321 ttcagccatt acactgcgcc cgcgcgccaa atcggcggag acgaaggcga agatatgctg
     4381 tccaacatcg ccggcgaaga agatttcaag caatacctgc acgcgcaagt gtgcgaacac
     4441 ccgctttccg accaagaagc cgcctgtgtc cacatcctta tcgatttcct tgacgagcag
     4501 ggttatctga ccgacagcat cgaagacatc ctcgaccata cgcccttgga gtggatgttg
     4561 gatgaagcaa tgctgaaaca agccctgacc gcattgaaaa aattcgaccc ggcaggcgtg
     4621 gccgccgccg atttgaacga atcgctgata ctgcagatag aaagatcggg cgaatgtgct
     4681 gccaaaccct ccgccctgca tatcgtccga aacgccctcg acagcattga cggcaaccgc
     4741 agccaaaccc tcgcgcgaat aaaaaaacgc ctgccccaaa ccgacagcgg cacactcgaa
     4801 gccgcactcg acctcattgc ttcgctcaat ccctttcccg ccgtcggttt tgcctcgtcc
     4861 acacccacgc cgtattctga cgaggcgctc gccaacctgc tggctttccg cggcatggag
     4921 gtttctcgcc gcaccattgc caaatacaga gaatcctttg agattccggc agcacacaaa
     4981 cgcaaaaccg cagaataatt gccgaataat cttataaaga caacaaacca aaagccggca
     5041 tttctgcgaa agcgggaatg acgg
//
